LOCUS       DAZKNO010000001        58022 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000001 DAZKNO010000000
VERSION     DAZKNO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.001"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..889
                     /locus_tag="ACG8MY_000001"
     CDS             8..889
                     /locus_tag="ACG8MY_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0429241.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            902..2512
                     /locus_tag="ACG8MY_000002"
     CDS             902..2512
                     /locus_tag="ACG8MY_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429242.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2589..3248)
                     /locus_tag="ACG8MY_000003"
     CDS             complement(2589..3248)
                     /locus_tag="ACG8MY_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0429243.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3531..4196)
                     /locus_tag="ACG8MY_000004"
     CDS             complement(3531..4196)
                     /locus_tag="ACG8MY_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0429244.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4343..4948)
                     /gene="dhaL"
                     /locus_tag="ACG8MY_000005"
     CDS             complement(4343..4948)
                     /gene="dhaL"
                     /locus_tag="ACG8MY_000005"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0429245.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4976..5986)
                     /gene="dhaK"
                     /locus_tag="ACG8MY_000006"
     CDS             complement(4976..5986)
                     /gene="dhaK"
                     /locus_tag="ACG8MY_000006"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0429246.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5961..6356)
                     /gene="dhaM"
                     /locus_tag="ACG8MY_000007"
     CDS             complement(5961..6356)
                     /gene="dhaM"
                     /locus_tag="ACG8MY_000007"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0429247.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6510..6830
                     /locus_tag="ACG8MY_000008"
     CDS             6510..6830
                     /locus_tag="ACG8MY_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0429248.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6942..7799)
                     /locus_tag="ACG8MY_000009"
     CDS             complement(6942..7799)
                     /locus_tag="ACG8MY_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0429249.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(7907..9211)
                     /locus_tag="ACG8MY_000010"
     CDS             complement(7907..9211)
                     /locus_tag="ACG8MY_000010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0429250.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9208..9717)
                     /locus_tag="ACG8MY_000011"
     CDS             complement(9208..9717)
                     /locus_tag="ACG8MY_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429251.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9892..12027)
                     /locus_tag="ACG8MY_000012"
     CDS             complement(9892..12027)
                     /locus_tag="ACG8MY_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0429252.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12110..13765)
                     /locus_tag="ACG8MY_000013"
     CDS             complement(12110..13765)
                     /locus_tag="ACG8MY_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0429253.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13767..14081)
                     /locus_tag="ACG8MY_000014"
     CDS             complement(13767..14081)
                     /locus_tag="ACG8MY_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0429254.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14251..14511
                     /locus_tag="ACG8MY_000015"
     CDS             14251..14511
                     /locus_tag="ACG8MY_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0429255.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14525..14839
                     /locus_tag="ACG8MY_000016"
     CDS             14525..14839
                     /locus_tag="ACG8MY_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0429256.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15569..16123)
                     /locus_tag="ACG8MY_000017"
     CDS             complement(15569..16123)
                     /locus_tag="ACG8MY_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0429257.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16249..18178)
                     /locus_tag="ACG8MY_000018"
                     /pseudo
     CDS             complement(16249..18178)
                     /locus_tag="ACG8MY_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18484..19200)
                     /locus_tag="ACG8MY_000019"
     CDS             complement(18484..19200)
                     /locus_tag="ACG8MY_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0429258.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19201..21024)
                     /gene="glpO"
                     /locus_tag="ACG8MY_000020"
     CDS             complement(19201..21024)
                     /gene="glpO"
                     /locus_tag="ACG8MY_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0429259.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21038..22534)
                     /gene="glpK"
                     /locus_tag="ACG8MY_000021"
     CDS             complement(21038..22534)
                     /gene="glpK"
                     /locus_tag="ACG8MY_000021"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0429260.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22670..24130)
                     /locus_tag="ACG8MY_000022"
     CDS             complement(22670..24130)
                     /locus_tag="ACG8MY_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0429261.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24120..25415)
                     /locus_tag="ACG8MY_000023"
     CDS             complement(24120..25415)
                     /locus_tag="ACG8MY_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0429262.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25747..26424)
                     /locus_tag="ACG8MY_000024"
     CDS             complement(25747..26424)
                     /locus_tag="ACG8MY_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0429263.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26454..27545)
                     /locus_tag="ACG8MY_000025"
     CDS             complement(26454..27545)
                     /locus_tag="ACG8MY_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0429264.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27646..28080)
                     /locus_tag="ACG8MY_000026"
     CDS             complement(27646..28080)
                     /locus_tag="ACG8MY_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0429265.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28664..30121)
                     /locus_tag="ACG8MY_000027"
     CDS             complement(28664..30121)
                     /locus_tag="ACG8MY_000027"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0429266.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30118..32013)
                     /locus_tag="ACG8MY_000028"
     CDS             complement(30118..32013)
                     /locus_tag="ACG8MY_000028"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0429267.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32071..33846)
                     /locus_tag="ACG8MY_000029"
     CDS             complement(32071..33846)
                     /locus_tag="ACG8MY_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0429268.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33896..35392)
                     /locus_tag="ACG8MY_000030"
     CDS             complement(33896..35392)
                     /locus_tag="ACG8MY_000030"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0429269.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35586..37208)
                     /locus_tag="ACG8MY_000031"
     CDS             complement(35586..37208)
                     /locus_tag="ACG8MY_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0429270.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37536..38849)
                     /locus_tag="ACG8MY_000032"
     CDS             complement(37536..38849)
                     /locus_tag="ACG8MY_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0429271.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39094..39318)
                     /locus_tag="ACG8MY_000033"
     CDS             complement(39094..39318)
                     /locus_tag="ACG8MY_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429272.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39338..39760)
                     /locus_tag="ACG8MY_000034"
     CDS             complement(39338..39760)
                     /locus_tag="ACG8MY_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0429273.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39903..40613)
                     /locus_tag="ACG8MY_000035"
     CDS             complement(39903..40613)
                     /locus_tag="ACG8MY_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0429274.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40631..41128)
                     /locus_tag="ACG8MY_000036"
     CDS             complement(40631..41128)
                     /locus_tag="ACG8MY_000036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0429275.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41165..41617)
                     /locus_tag="ACG8MY_000037"
     CDS             complement(41165..41617)
                     /locus_tag="ACG8MY_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0429276.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41752..42357)
                     /locus_tag="ACG8MY_000038"
     CDS             complement(41752..42357)
                     /locus_tag="ACG8MY_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0429277.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42807..43427)
                     /locus_tag="ACG8MY_000039"
     CDS             complement(42807..43427)
                     /locus_tag="ACG8MY_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0429278.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43429..44298)
                     /locus_tag="ACG8MY_000040"
     CDS             complement(43429..44298)
                     /locus_tag="ACG8MY_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0429279.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44321..44728)
                     /locus_tag="ACG8MY_000041"
     CDS             complement(44321..44728)
                     /locus_tag="ACG8MY_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0429280.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44747..46036)
                     /locus_tag="ACG8MY_000042"
     CDS             complement(44747..46036)
                     /locus_tag="ACG8MY_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0429281.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGDFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46063..46365)
                     /locus_tag="ACG8MY_000043"
     CDS             complement(46063..46365)
                     /locus_tag="ACG8MY_000043"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0429282.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46390..48483)
                     /locus_tag="ACG8MY_000044"
     CDS             complement(46390..48483)
                     /locus_tag="ACG8MY_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0429283.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(48972..49736)
                     /locus_tag="ACG8MY_000045"
     CDS             complement(48972..49736)
                     /locus_tag="ACG8MY_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0429284.1"
                     /translation="MKKINYLAIILLVGLCLFALPPQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49847..50716)
                     /gene="mdcB"
                     /locus_tag="ACG8MY_000046"
     CDS             complement(49847..50716)
                     /gene="mdcB"
                     /locus_tag="ACG8MY_000046"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0429285.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50741..51817)
                     /locus_tag="ACG8MY_000047"
     CDS             complement(50741..51817)
                     /locus_tag="ACG8MY_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0429286.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51841..52434)
                     /locus_tag="ACG8MY_000048"
     CDS             complement(51841..52434)
                     /locus_tag="ACG8MY_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0429287.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52438..54114)
                     /gene="mdcD"
                     /locus_tag="ACG8MY_000049"
     CDS             complement(52438..54114)
                     /gene="mdcD"
                     /locus_tag="ACG8MY_000049"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0429288.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54104..54403)
                     /locus_tag="ACG8MY_000050"
     CDS             complement(54104..54403)
                     /locus_tag="ACG8MY_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0429289.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54505..56151)
                     /gene="mdcA"
                     /locus_tag="ACG8MY_000051"
     CDS             complement(54505..56151)
                     /gene="mdcA"
                     /locus_tag="ACG8MY_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0429290.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56158..57069)
                     /locus_tag="ACG8MY_000052"
     CDS             complement(56158..57069)
                     /locus_tag="ACG8MY_000052"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0429291.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57189..>58022
                     /locus_tag="ACG8MY_000053"
     CDS             57189..>58022
                     /locus_tag="ACG8MY_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0429292.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TM"
BASE COUNT        16277 a        12408 c         9994 g        19343 t
ORIGIN      
        1 ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg gtcgtttcga
       61 agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat ttatcggacc
      121 aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa aaaaagacgg
      181 cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta tccatgaacg
      241 tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag gagcgatcat
      301 cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg aactgattca
      361 acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa atcgccaaaa
      421 agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg atgagcctac
      481 ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa aattgaaaga
      541 acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc gactagcgga
      601 taaagtcgtg atcatacgcg aaggaacact cgatgaagaa ggaacactcg atgaattgcg
      661 tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc acttagcaca
      721 attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg ctgctgataa
      781 cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa aatttgaatc
      841 tattccaccg actcttgagg atttattcat gagacattat gaaggatgag gaggaagcga
      901 aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta gccgtgactg
      961 gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt ttgtccctgc
     1021 gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct tgcaaaaccc
     1081 ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt atactttagg
     1141 agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg tgatctctgt
     1201 tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg aacttattcg
     1261 ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa gtattctgat
     1321 caatttgctt ttagcaatat cgatcagttg tacgatgatt tttttccaag cggattcgat
     1381 cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga ttttggggag
     1441 tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga atggtctgtc
     1501 tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag ctgattcctc
     1561 cctttcgatg tggaatcctt taggctggtc ttatttgacc tatccattta caactaacaa
     1621 ctggattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca gttcatttgt
     1681 tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag gaaaaccgta
     1741 tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata aagggatgac
     1801 aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta tctacgggaa
     1861 tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc aatctggcgt
     1921 ctcaattgaa aaaaacttta caagcacgat catggtcgtc ttgatttgtc tagctgctat
     1981 tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac gtatgaatca
     2041 gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt tactagctat
     2101 tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt ctgctttggc
     2161 ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg ggttcaattt
     2221 cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat ggcttcctcg
     2281 aatcggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt actttggcgg
     2341 aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg ttccacggat
     2401 gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca gccttgcact
     2461 gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat aataaaaaag
     2521 catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt tggaggtcat
     2581 ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc tcgattcttt
     2641 ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt ttcgggggtc
     2701 gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg atccttccct
     2761 gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt ttccatcatc
     2821 aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc taaaagtttc
     2881 agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct caatatgcaa
     2941 atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc tagcaactgg
     3001 ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt tccgctagca
     3061 gataattgat acactttcat actgccggag gccacgatca ctaattgagg ttctccattc
     3121 ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc gtgtactttt
     3181 tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca taaatgatca
     3241 tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca taacttccct
     3301 tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc agataccagc
     3361 aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag accttaagta
     3421 attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac aaaccttcac
     3481 tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt ctattgaaat
     3541 ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc agcggttatt
     3601 tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg aaaggttcgg
     3661 tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata gtcagacaaa
     3721 gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc cttgatatcg
     3781 actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc aatgctacca
     3841 tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg tttttgattc
     3901 aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta cgcgctccaa
     3961 aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt gctcatgccg
     4021 tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt ttgccagtgt
     4081 tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc tttgagctgt
     4141 ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag ttccattgtt
     4201 tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga caaatagagg
     4261 ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa ctgcgatgag
     4321 catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc gttgaaaata
     4381 aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct cctagatagg
     4441 aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg tcaatgattt
     4501 cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca tctaacatcg
     4561 ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc tgctcgatca
     4621 cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct ttcatcatgt
     4681 tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat atcatcgcca
     4741 tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga aaagcttctt
     4801 cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca atcggggtat
     4861 ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc gtgagccatt
     4921 cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag attttttacc
     4981 atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc gtcaagtcga
     5041 tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg acttttcgga
     5101 aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg aacacgaact
     5161 gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct tttggtaaat
     5221 tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta gaaggctgca
     5281 tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg atctcatctt
     5341 ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga agcgctagac
     5401 cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca gctagggaag
     5461 caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct gcaactccgc
     5521 gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg atcacacttt
     5581 ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc aatgcatccc
     5641 ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc ttaatagccg
     5701 tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct gcacttaaca
     5761 tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca ccagaaacca
     5821 aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct acacgatgaa
     5881 caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata ttgcttggat
     5941 cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg tgcttctgtt
     6001 aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa agctccttcc
     6061 actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa catgtctttt
     6121 gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata actagctgca
     6181 tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat ctcgccattt
     6241 attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc aatcatttct
     6301 ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt tttcacaaga
     6361 atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa tgaacaattc
     6421 aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg aagtttgtta
     6481 tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac agtgtagagg
     6541 gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact gggatccatg
     6601 catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca aaaagctatg
     6661 aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa cgtgcaaaag
     6721 cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta ggcagcgaca
     6781 atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa gtaggggaca
     6841 tctgttttaa taactaaaga gagatgggac atgtagtggc actcaagaat tagatttttc
     6901 agtctaactt cgaggtactc taccttccca tcctctcttt tttatttctt tttgcggatg
     6961 attccgtaag ctgctgcgac taacacaccc gcccctgcta aggaataact gacagaagct
     7021 gttttttcat ttgtttttgg caaagtctga cttgttgatt tggatggctc tgtcgtttgc
     7081 tgaactgttt ctgtcgtgct tccagaggtc actgattttt ctcctgccga atgcaaaata
     7141 tcttttattc gttggaaact agctgctaag tcagcttttt ttgcttcaaa atcattagca
     7201 actgtttgtg cttcgctgat ctttccgtgg attgcttgac tggccgctgt gatatccgct
     7261 tgagtttgac ttagctttcc agaagctgtt tgagcaacct gatttaattg ctgattgaat
     7321 tcttttgctt gttggatctt cccgtcaaga tctgtttgga cagcttccgc ttttgctttc
     7381 gcttcggcaa gctttttctc aagttgtgca cggtcatttc ccactttatt tttaagatca
     7441 gctaaatctt gatcgattgc tgcttggtct tctttcaatt tgttttttgc agcttctaat
     7501 gcttcttcga gttcttttct tttattttcc aagtcttgca aatgatcgat ttggtcagaa
     7561 gaggtagaac tatttattga tgtatcttct gaagatgaaa tcgtttctcc tgaattatct
     7621 gtcggtacat tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc agaagagctt
     7681 atggttgttg aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc tgtctgtccc
     7741 aataagaaaa tactggaaac agcaattggc cagattaacg gcttcattct ttttttcatc
     7801 acttttccca tccttactac tttttaacta atttcttatt ataagcaaaa atgtttggaa
     7861 gaaccataaa cacaacaggc tgtttgtcta aaaaacggtg catcttttat ttttttgtac
     7921 gttccgggct tttccatgtc ccgcttccat aacctaaaat cgttttgact gctggaatga
     7981 aagtcgtcac gatcgttatc gcgttcacta tccagaacaa aagcatatat agtggtgcga
     8041 aaagaaggta tttcaatttt cttcgtcgat catctacaat cagcgaagct agcaactgga
     8101 aaaatcctgc gaccatttca aagcagatga acacaaaact gaatgtaaaa gtaatataaa
     8161 tctgttcgta gtttccttga tatacataat gcccgattaa tccagcaaaa agaactacgg
     8221 aaatacaaaa gaatattgac cagacaatac tcaatgtttg atcaatgaag atgatcgttc
     8281 ttccaatatg tttaaaagga tggagcatca cttttttgaa attcgtcagc cagacttctg
     8341 ttccaccttt tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc acaggtactt
     8401 ccatgaaaaa gataatctca ggagcaaaaa cagataacca gtcatttaac tgatgatccc
     8461 aagcaatact gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca tcgatcaagg
     8521 cttcttttcg atacatcgta tttgctccac tgtacgcata aagaccaccg aaaacagctg
     8581 actgtgttcg tttaatgatg cccacgatac ttgagaattc aaccgtttga gattttgcaa
     8641 tcaatttggt acgattttgg acatccatat ttgcagtgac cgcagcaata tgtcttgcac
     8701 cagggcgaat gaaataattc acatacctaa tcagtgcatc tggctccggt accgtgtctg
     8761 catcattgct taaaatcaat ttcccttttg caaaggcaag tccgatatta aacgcgtggg
     8821 cttttccttt atttttttcg atccgaacca cacgaagatt agcgtatttt ttcatcagtc
     8881 tagctaatat ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat acctcatagt
     8941 tgtgataatt caatttcgtc atcaaatact cgattgtata ttcgatcaca atttcttcat
     9001 tatgggctgg caccatgatc gtgatgaacg gttcgacact caagggaaca tctacccatt
     9061 ctttttgctt atgtttgtat aaaaacacat aacaccagac accaataaac catacaaatc
     9121 cgcctaaaat cggataagaa acgagaaaaa aatacataat agacaaacaa gtatctaaga
     9181 ataaattacc tgtatgggtg attgagatca tttcagttcc accccatttt ctttatacaa
     9241 atctgcaatg aatgtcgtat ctagattttt ctcttcggag acagaataaa aacggaccgt
     9301 ttctctctct tcttttgttc caaaacgttc cgtatacact tcttcaagta attcttgtct
     9361 ttttttcaat ttttcatctt catattcaaa agatttattt acttttttgt atagatttcg
     9421 attgttccac ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa tcaatacaaa
     9481 aaagccgatt aagaaataca gcattttctt ttctgataaa aaagcgtaca aggaaaaatc
     9541 cttggctctt tctggtctcg ttaatgggaa aaagacccat ataaaaggaa gaacaacgat
     9601 catccatcct aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat taccttttga
     9661 aaaatactta tctttcacca aaattgatgc aacttcttct cttgttactt ttttcatatc
     9721 ctcacctcac gtttttttgt tacgacgtag gagtttacca tacatttcat ttcttttcac
     9781 ataaagtgtt cctaaatttt tcctaagaaa aagaacaaaa catttctcat cagcaaatga
     9841 aaaatatttt gttcttttta tcagttgttc tttttatttt ttatctctta attatgttgc
     9901 tctttccaat acgacaatgc ttttcattcc tctagatatc gccgtgtaca tttgtctgcg
     9961 tcttctttgc tcatctccat cgttttcaaa tggatataag attacatgat caaattccag
    10021 tccttttgct agactcaaag gaatgatttg gcagttttct aatccatatg aatttttttt
    10081 aatcgattct tgtagcgttt gggtttcctc ttctgtttgg gtgataactg ccgctttttc
    10141 tccttttaga gaaaccaaaa tctcagctag tattttcaac aattcttcag aggatcgaaa
    10201 agagcggtat tcgggttctt ttccttgcgg tcggatcgat acgatgtcga tcttctcttg
    10261 atccacagca taggttttga aaagctctgt gattgctccg ctagaacggt aactatttct
    10321 aagatcatac cgatggatcg gcagatggta gtcgtcgaaa caacgcataa tatcagcaaa
    10381 agtagtggaa gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa agtgagtttt
    10441 tggaaataat tcgatcaata gacctaattg tgccagggta taatcttgta cttcatcaat
    10501 caatagatag cgaaatgctg gtacatttac cttttcaaca agtaaatgac gaatagctaa
    10561 tataatgact gcttcatcaa cagtacgtgt gggttgccaa gcataagctg agccatatcg
    10621 tttttcataa attctttcaa acaactgatg ctcatccacc catgccattt cttcgatttg
    10681 tctggttatt tttcgatatt ttttttgtaa aagtttcctt gcataagcta caatactttg
    10741 ttccgaatca tcagaaatca atttaccgaa taattccaac tgcctatctt ctgtcaacga
    10801 gagtacctga ttctgcacag caggactttt tgcttgtttc aaaagatgat tcttccattc
    10861 gctcatcagt gcctttttcg ttgcttgaag cctatcgatc aatttaggat gagcaggaac
    10921 tttctcatat attttttcga tcgttttttt agaaaggact gtttttccat ttttagtaag
    10981 tccacggata aaattcggat gatctaaaaa caaaggatct gattgtttta gattatcgat
    11041 aaactttttg ctacgcaaac gttctgtctg ctccgataca ttttctcccg tgatacgttt
    11101 gaaataagcc tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca gctgtatgat
    11161 ggttagattc agtggatttc tttcacctaa tgatggtagg acattcgaaa tatagtcgat
    11221 aaaccgatga ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat ctcgatataa
    11281 atagagcaga tacgcaatac gctgcatgat agcagaggtt ttaccacttc ctgctacgcc
    11341 atttaccaag atattttgac ttttcacatc ccgaatgatt gtgttttgtt ccgattggat
    11401 cgtagcagtg atagccttca tttcattggt ctcatcttgg cctaagactt ctaataatac
    11461 gtcatcttga atagctacag acgtatcaaa aaacttgacc aaacgatctc tttccaaaag
    11521 aaattgtcgt ctttctttaa taaccgtatc gattttattt tgacgaaccg tataagaaga
    11581 tggaccaatc atgttattgt aaaaaagttc ggcaattggt gaacgccaat cataaacaag
    11641 attttcatct gcatcatctg tgaaatttgc tgtaccgata taaaaggatt cttttggctc
    11701 attatccaga aagtctacaa cgacttttcc aaagtaggga cttttcaata aacgttcgat
    11761 ctttgctagc tgattttctg cgtttttctg tttgatattc atctgatcga tttctctatt
    11821 tttcatttca atcatcgaaa aagtatccag gttatctaag taactgtcaa aattcaatcg
    11881 aagctcgcca gccatttctt tcaacgactg gtttccttct atttttgccg tatccatcga
    11941 ttcccgcagt tcgttttttt tatcaattag cttttgatac acgttcttca gatatgcttc
    12001 ttctaactgt ttttgtgtgt tgaccataca cattctcctc tttttttaaa gtggtggaac
    12061 cgattagttt acctctttta agagtcgaat gcaagctttt taacgagtat catttgttag
    12121 gatatatcag ttcttcaggt cggacagttt gatataaaga aaatgcacca tcaaggtttt
    12181 ttacgtggta gccagactgc ttcaagattc tttccgctag atagctacgt aatccgctat
    12241 gacagctgac gatatattct tgttggctgt ctaattcatt taatcggtcc cgaagttcat
    12301 tcaaagggat atgtttggct tccttgaatt tgcccttttc caattcttct gctgttcgca
    12361 catctaataa aatttttcct tttgctagtt ctgtcggcaa ttcatgccat tgtacggttt
    12421 cactaagccc ttctgcaatg ttcattgctg catatcctag catgtttact ggatctttgg
    12481 ctgagccaaa tggcggtgca tacgtcaatt ctaattccgg taaatcgaag atcgtcaaat
    12541 gccctttgat tgctgttgct aaaatatcga tccgtttatc cacacctttt gccccaacac
    12601 cttgtgcacc ataaatctcg ccagtctctg gatggaagat caacttcagt aaaatatctg
    12661 ttgcttctgg ataatagctt gcatgatctt ttcctgatac atgaatgact tcaaaagaca
    12721 gttcgttttc tttggctgtt ctttcactta aaccggtgga tgcggcagtc attccaaacg
    12781 ttcgtataat tgctgttcca ataccgcctt gatttcttcg agaaattccg gaaatcgtgt
    12841 ccgcaacttg tctaccttga cgattggctg gtgaagcaag agaaataagt gcatcttttc
    12901 cagtgatttg ctgttttaca acgatagcat cccccaccgc ataaatatca ggaatattcg
    12961 tttgataacg ttcgtctact aggatacccc cgcgtatgcc taattcgatt ccagcggtca
    13021 ctgctagctg gttatcagga cgaacaccaa tggataaaat aactaaatca gcagggatgg
    13081 tacgcccatc atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg acagcatgag
    13141 aagtaatcac ttctactcct tgatgagaca attctttttc tataaaagct gccatttctt
    13201 catccagtgt tggaagaata tgtggtgctt gttcgatcac catgacttct aaacctcttc
    13261 tttttaggtt ttctgccatt tccaatccga tgaatcctgc accaacgatc acggcacgct
    13321 ttggctgttt ttctaatgca tgcatcactt tatcgatatc tggtacattt ctaatagaaa
    13381 aaacattgtc ggcttctgtt atccctggaa tcgatggaac aactggtttt gcaccaggag
    13441 ataaaaccaa tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg acttctaccg
    13501 ttttgttttc gggaaagatg gctgtcactt catgatttgg acgcacatct aaacaaaaac
    13561 gagcggataa tgattctggc gtttggacaa gaagattttc ccgttcactg atttctcccg
    13621 aaagataata aggcaagccg caatttgcaa aagaaacata cggtcctttt tcaaaaacaa
    13681 cgatttctgc atcttccatt aatcggcgaa gccgtgtagc agcagacatt ccacctgcta
    13741 cacctccgac aataacgatt ttcatcttat ttccctccta ctgttcgatc aaaccattga
    13801 ttcatgccac cacgtacatt cactacatca taaccagatt tctttaattc tttagctgct
    13861 tgtttactcc tcatccctga ttgacaaatc acatagacgg tcttttcttt gtctccatca
    13921 tatctgttta tactttgtaa tggaacattt ttagctcctt ttatatgccc gccccggtat
    13981 tctgcaggtg ttctaacatc aagtaacgct ggattttcca aaagcttttc ctttaattct
    14041 gggactgtta tcgattttat tgattgaaat agcaaattca ttttctgcac ctcatttaca
    14101 tttttataaa tacctcatgg ggtatattaa acaggagctt tattaatatg tcaaccaaaa
    14161 tgactcttca tttttttcac tactttttat atttgcataa aataccgata aggggtatta
    14221 tctaattata aaaatagagg agaatgtgtt atggagattg ataataaaaa tatcctgaac
    14281 cgtttgaaac gtacagaggg acaaattcgt ggtatccaaa aaatgatcga agacgaaaaa
    14341 gaatgtatgg atgtcatcac tcaattaagc gctgttcgtt ctagtattga tcgcgtaatg
    14401 ggcatgatcg ttgcagataa tctgaaaaat tgctttgaga aacctgatag taatccagaa
    14461 gaacaagcgg ctaaactaga acaagcgatc aagatgatta ttaaaaagta aaggaatgat
    14521 ttcaatgaaa gtcgttttcc atattgatga attagaaaaa tggtcagaaa cagggaaaaa
    14581 cgtaaaaaac ctgataaaag cttctccaga aacgacgatt gtcgtatctg taaatggcat
    14641 cgcgattacc ggttatctag actctgccaa tgcagaattc ttagatttgc aaggagtcgt
    14701 ttttcacgct tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt ctttacctga
    14761 acaagtaatc gttgtaccag caggtgtcct tgatttagtt gaactgcaat cccaaggtta
    14821 tgcttatatc aagccttagt cagtagaggt tgcgacagaa gtgatcagct tcaagaaatt
    14881 tcggcttatt tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt tgtgaaaacg
    14941 ctgtcctgca tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcgttt
    15001 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    15061 atcagcggtt tgacctaagt attagagaaa aaaacgggaa aatagctcct catattttgc
    15121 cgttttttga gctaataccg aaggtcagct gattgaacac cgtttaagga agagattgtg
    15181 aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggatt tctacatttt
    15241 tgcataaatt tggcttaata ccgagaaact ggcttttgaa taccgtttaa ggaagaggtt
    15301 gtaaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag ctgctcatat
    15361 tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt accaagaggt
    15421 tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa cttctcctgt
    15481 ttttggttcg gttggcttat tatcgagaag tagctactgg gagtctattt atcaaaaaaa
    15541 ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt cggtactcga
    15601 cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa atgactctca
    15661 cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca tagatgacag
    15721 cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta gatagcccga
    15781 ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac aacagccaaa
    15841 gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat ttatctgcaa
    15901 acggatcaag caccttccct aagtctgtta cctgattata tctccgagca atgaccccgt
    15961 ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa aatgactgtc
    16021 tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat aaaagattcg
    16081 gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg ccctacctcc
    16141 tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg atttcactgt
    16201 ttttattttt ctttacagac actgatatta tgagctaatt tgagataatc acttgctgtt
    16261 ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta gtactttatg
    16321 agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat aatcgataaa
    16381 cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca ccagacgaac
    16441 acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg tatcttcacc
    16501 tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc gatcaccttt
    16561 tgcaaagagc tgacgttctt ttttattctc cttcctattt gtaactgctg tcttaggaag
    16621 aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg cttgatggat
    16681 caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt ctcctgtttc
    16741 ttccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc atagcagccg
    16801 gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg gtcgtggcaa
    16861 tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt gcagcccctt
    16921 catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc ctttttatct
    16981 ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac gcattttcta
    17041 aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc tctgtccctt
    17101 cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg actgtcttca
    17161 acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga tagtctttac
    17221 cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg ccttgcttga
    17281 taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct cggctaaatt
    17341 ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa tcgatcactt
    17401 cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct tcactgattt
    17461 cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt ggttcaataa
    17521 aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta tttttatagc
    17581 tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct tcttggatca
    17641 tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt tctttttcga
    17701 ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca ctgactttat
    17761 tattttttat tttctggtca atcaactctt ctacatttat caaatcagga agatgtttgc
    17821 tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt gtgatcatcc
    17881 gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga atcaaccctt
    17941 ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga acatgctgac
    18001 tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag acagtttgta
    18061 ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca attgctcttg
    18121 cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg ttgtccatat
    18181 tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag agataaataa
    18241 aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac aaagatattg
    18301 ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat tggctattcg
    18361 acttactgat tagataccag ctacactcac aaaacatacc ccattcttct aggtcttatt
    18421 tattttccac ttaactatag caagacacat tttggttgtc aaactttagt atgttttata
    18481 gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca ttggacctgc
    18541 gattggtacc catgaatatg cccaatctga agttccttta ttagctattg gcaataccgc
    18601 atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg ttggccctcc
    18661 taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg ttccaggtgc
    18721 aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac caattaattc
    18781 tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg ttcctaaaat
    18841 cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa caagaatggc
    18901 ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat tccaactaat
    18961 tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg ggcccatgaa
    19021 tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga tgacaatcca
    19081 gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac catctccaag
    19141 caaaactagg atcagtgtcc caataaactc ccccatgatt tgagtcatat cactgttcat
    19201 ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt ctaaacgttt
    19261 agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg catcgatcac
    19321 aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg tacgacgaat
    19381 gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta accgagctga
    19441 ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa agatacgtcg
    19501 ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta aaccggctgc
    19561 tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat cgaagtttcc
    19621 acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt attcttcttt
    19681 taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat cggtgatttt
    19741 cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac ctctggaaat
    19801 cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt cttctacttc
    19861 agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt tattgaagct
    19921 cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat taccaatcaa
    19981 cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat ttgcttctgg
    20041 atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga ctttcggatc
    20101 cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt tttctcttgg
    20161 aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg gttgcggtac
    20221 aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca ttttaggaga
    20281 gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac tcgtattgat
    20341 cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag ctttgacgcc
    20401 aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta ctagataagc
    20461 cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat tgttgcggaa
    20521 atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt ctctttccaa
    20581 tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat ttgccagttt
    20641 gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg ctccttcgcc
    20701 ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa tactttgtac
    20761 gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct tcagataacg
    20821 aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa aatcctgcat
    20881 ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac cggcaccagt
    20941 gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt gaatgctatc
    21001 ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct tgggtagctt
    21061 ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc atttgaactt
    21121 caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc caataaccga
    21181 cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt tctgctcgtt
    21241 ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg tttgctgcac
    21301 cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc gtatcgatga
    21361 tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct tcttttgtcg
    21421 taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa ggagcaccta
    21481 gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt ttcgctgcgt
    21541 cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat tgaatcgaag
    21601 aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt atgccgtaac
    21661 caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta ttcatgacaa
    21721 taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc atttgtccga
    21781 ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt ccataaaaat
    21841 gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt aacatcactc
    21901 gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg atattgaaca
    21961 acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg gtcagtttcc
    22021 atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct cgttcttgcg
    22081 cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag gcatcgatca
    22141 ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag ttatctgcaa
    22201 ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt cctgtatctt
    22261 tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca atctgatttg
    22321 gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa ttccagattt
    22381 catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac tctttttgag
    22441 aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta gttgtacctt
    22501 ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt atatgcaaac
    22561 gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt caaaatttta
    22621 ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat caattctttt
    22681 cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt gacgcaaatt
    22741 ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc accaaatcat
    22801 attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc agttctctga
    22861 tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg attccaatca
    22921 ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac tggatcatca
    22981 ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc gctgtttcct
    23041 gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc tgttccaaaa
    23101 gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca ttgttgatct
    23161 gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga ggacgataat
    23221 gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac tttttgctcc
    23281 gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa acaaaaaagc
    23341 tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta agatgcatca
    23401 cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat ttcttttctt
    23461 tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc ccgagccagc
    23521 aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg tccaatccta
    23581 gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga tcttccggga
    23641 tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg tataattgac
    23701 cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg atctttcgaa
    23761 aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat acttgctgat
    23821 gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa aacaatagaa
    23881 ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca tttcgctgta
    23941 cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa tattgatcaa
    24001 acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct gttccccctt
    24061 gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct aataatgctt
    24121 caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac acggtattct
    24181 ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc ttctactgca
    24241 attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg tgctccaaga
    24301 atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt tattgctgta
    24361 agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct ctcttcttct
    24421 gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg acgataggtt
    24481 ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc tgtacggaca
    24541 gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt tacttggatc
    24601 gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc tacccagatc
    24661 gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg acgagaatga
    24721 ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac aatagcggtt
    24781 tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc ttgatttttc
    24841 atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag aattgacttt
    24901 cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc tatttcgatt
    24961 cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt ttgctgaaga
    25021 gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc agaaatattt
    25081 cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa tttttttcct
    25141 cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt gtaaacatgg
    25201 attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt ttcactggta
    25261 acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt cgattgcttt
    25321 tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc cgcatgaatc
    25381 cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc ttataaggtg
    25441 ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt caaccttatg
    25501 atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag gtctgcttct
    25561 caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg tttcaaaaaa
    25621 tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa taggaaacag
    25681 aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata gacacatcat
    25741 ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag cagattgagg
    25801 attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag ctgctccaga
    25861 ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta cttcattcgt
    25921 tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc cagctaaaat
    25981 agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac aaacagctga
    26041 gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta ttgcttctgg
    26101 aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat catttgcatc
    26161 cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt catcttcata
    26221 ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag gtacatatcc
    26281 gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat cataaaaatg
    26341 agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact tttctacatt
    26401 tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt gcgttattga
    26461 taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt atatgcttct
    26521 tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc tgcccagtca
    26581 ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa tacctgctct
    26641 acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc ttgaatcgtg
    26701 ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc tattcgatca
    26761 tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg ttcaagaagc
    26821 cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga gaaacgatac
    26881 cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt tgttaataat
    26941 tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc tcgtctattc
    27001 gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa ctcgattcca
    27061 tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat tgtttcaacg
    27121 atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc tgcaactgca
    27181 cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg tccaccgtgg
    27241 aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag ctcacttaat
    27301 cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt tccgatatct
    27361 tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg tttataataa
    27421 gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt cataggcgac
    27481 agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg atcaaattgt
    27541 gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca tctatcatta
    27601 aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt ttttttgtaa
    27661 cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat ttcccaaaac
    27721 tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag caaattggcg
    27781 tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt tgacgtatcc
    27841 tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat agagcatttg
    27901 tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa taaaaccata
    27961 agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca acatgcgatc
    28021 tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg gcttttccat
    28081 ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt caaaaaacac
    28141 ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat cttcaaagaa
    28201 tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg ttgtgaaaaa
    28261 gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
    28321 aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag aggttgtgaa
    28381 aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttgt
    28441 cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga gaggtcgtga
    28501 gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc tcatgtttta
    28561 tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt cgtaaagaag
    28621 gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg atttcgccat
    28681 ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt ttctttgtgc
    28741 cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac atttgtgaag
    28801 tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa tctactccat
    28861 ctttgatagc ttccttcatc tgctcaatat gctgcttcaa atagtcaatg cggtaatcgt
    28921 cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt tcagctatga
    28981 acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa cccttaggat
    29041 caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct ccagttatta
    29101 agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta gacatgtagt
    29161 agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca cctacttcta
    29221 tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga taatatcctc
    29281 ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt ttttgaatat
    29341 catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa ccgacttgtg
    29401 aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata aactgatggt
    29461 gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt actcctacgc
    29521 taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa tattttactt
    29581 tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga ataagctttc
    29641 gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa tgggaaagag
    29701 aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca taaaaaaata
    29761 atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc catgcgatag
    29821 acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct ttaaagtgat
    29881 gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga tcctgcgccg
    29941 cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct ttaggtaaat
    30001 cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca tcccatgcac
    30061 cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga aattttttca
    30121 tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat gactagtaat
    30181 gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat catatccctc
    30241 tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta ctttttttcc
    30301 atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa taccgatatg
    30361 gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg ttggaaaaac
    30421 tgttgaaact tccccgttta cagaactgta gatttttccg tccgttggaa tgatcgcaaa
    30481 cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata aatcaattat
    30541 ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt cgatttcatc
    30601 cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga aataactagc
    30661 aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac ttcttgtagc
    30721 aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag ctccacatat
    30781 acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag caggttcagt
    30841 gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt tcttattttt
    30901 cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag tcatacatgc
    30961 tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg gtacaagtcc
    31021 ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag caccagataa
    31081 aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac ttgcataatc
    31141 tgcaattgga cctacaacta ataaaataat tgggaccatc acgatgatac aaatgaatgg
    31201 tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct caatataaga
    31261 aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg ctagaatcac
    31321 gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa tcgtaggata
    31381 aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact tttttgcagc
    31441 agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg cattcaaaat
    31501 gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg cgagtactcc
    31561 tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag cggcgatcat
    31621 gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac tagatgcatc
    31681 atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg catttcctac
    31741 tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta gttgctctac
    31801 cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg ttgcacaatg
    31861 gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt tcgctaattg
    31921 tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc ttcttacaag
    31981 ataagtatag ttcttagaaa acggtttctc cattcaatta gtaccccttc taatagcgaa
    32041 cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca ctgcttgaaa
    32101 gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac gcttgatctt
    32161 cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg tttctacttg
    32221 gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa ctaggtcaga
    32281 actaggagag attagttgct gaatggaatc tatcttttta tggattttta tttgattttc
    32341 ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta tggtcagctt
    32401 ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac taggtagata
    32461 aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg catcatattc
    32521 cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc attctttgat
    32581 ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac taattggctg
    32641 gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt cggcaagata
    32701 caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta ttgggaagaa
    32761 acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta caaaatgatt
    32821 catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg ttagcaaaaa
    32881 ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa tcgtttgctc
    32941 aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca tcaaataatt
    33001 taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag aatagggtag
    33061 ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg tatacctgtt
    33121 tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc gagaaaatcg
    33181 tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt ttaatgtctt
    33241 ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca accttgcgtg
    33301 ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat tttcttcagc
    33361 aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat tgcgctcgat
    33421 ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa agaaaatgtc
    33481 tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca caatagttga
    33541 agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta tgatttgctg
    33601 tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg actggataat
    33661 gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg ttttcttgtt
    33721 ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat ttcttaatac
    33781 tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc tctttgttat
    33841 aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt aaaaatcatt
    33901 tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt ttataatatt
    33961 caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt ctatccacat
    34021 aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata tcgattggtc
    34081 cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca atttcctgaa
    34141 gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat tcatctggtg
    34201 cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa cggtctgtca
    34261 attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa ggttcgggag
    34321 atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct acgcgcattt
    34381 ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa tctgccgtgt
    34441 tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct tcttccttcc
    34501 atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag aaaaaccatg
    34561 aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca tatgcataag
    34621 tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg attttcttta
    34681 ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga taaatcactt
    34741 tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta accaatccag
    34801 cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc caatatttca
    34861 ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag tcgattaatt
    34921 ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca taatgactaa
    34981 gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg tcataaaata
    35041 cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt gcccatgaga
    35101 tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc tcttcaaatc
    35161 gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct aaagcaatct
    35221 cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg atcccgataa
    35281 gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca cattgattag
    35341 cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc ataactatct
    35401 ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa acaagatatt
    35461 ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt tttaatattt
    35521 atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc atctaagcaa
    35581 acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac tttgccgtct
    35641 ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg tggaatagtg
    35701 aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc ttcatatatg
    35761 cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc tccccagacg
    35821 atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa aacagaatct
    35881 tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac tcgaagccat
    35941 ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg cgtcctagca
    36001 ttgtctcttc ctttcgcctt gattgattcc aagatttcct cttttgtaaa tccagaagaa
    36061 atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat atcatcgatt
    36121 gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc tttcatcata
    36181 tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc atttccccaa
    36241 cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca tccttcatca
    36301 cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact caagggtttc
    36361 aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa ttggaagacc
    36421 atggataatt catttctttt aggattggaa taaagtttag cgatttcggt agttgctccc
    36481 catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt catttcctga
    36541 agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc tttgccaatc
    36601 atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa attcatcatt
    36661 tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg acgcttgcta
    36721 tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga accactgaat
    36781 attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat ataatagtcc
    36841 cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc atcagaagta
    36901 tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc ttcaatcagt
    36961 tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt actgatatca
    37021 tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc atcaatacct
    37081 aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat accatcttga
    37141 tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg ccaccatttt
    37201 tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat catttatttt
    37261 agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg ttatcggtaa
    37321 cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga aaggattgat
    37381 ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca ctcagctgtc
    37441 ccttggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt ttttttctct
    37501 aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa tatccacatg
    37561 aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt tcctctttta
    37621 gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc tgaaatacta
    37681 taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc taagtttccc
    37741 cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa tgctggtgca
    37801 gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa caagaagaaa
    37861 atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc agctgatgat
    37921 tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat agctgttgaa
    37981 tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa acctgcttgg
    38041 tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa gaatgtcagc
    38101 actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc aactaaaaat
    38161 gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat caaacctgtg
    38221 accagaccta aaatcgttgt agctgccatc ccgatgaaga tgccacctgg tacacgccga
    38281 acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga accaacgttg
    38341 agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc ttcgctcaga
    38401 ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa atctgctggg
    38461 atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa aatcaaagaa
    38521 gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa acaaacagaa
    38581 taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata acgggctaat
    38641 acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa cactgctcct
    38701 tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata agccattgag
    38761 ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa tttttctatt
    38821 tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga acattataaa
    38881 taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat atactaaaac
    38941 aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc catctgatta
    39001 aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt tctttattgt
    39061 gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga ttttaccatt
    39121 tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat tatttagtcc
    39181 gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc ctgatttgag
    39241 attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat aaccttctaa
    39301 tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac tcagcttttc
    39361 ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg ccattgttat
    39421 ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca tccgtaaaaa
    39481 atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca aatacttttg
    39541 ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta aattcttctg
    39601 gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat cggcaaatca
    39661 tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt tgacccttct
    39721 ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga aagcgcaaac
    39781 accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat tgctttcctc
    39841 cacaaacttt ttttacaaaa aaaaaaacgg caatgaatgc cgctcttctt atttcgattc
    39901 ttttataatt ctgcttctga ttcatctttg gttagtgtgt gtctaacact agttacgcca
    39961 aatgtttgat cgattttttc cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt
    40021 tcagcattga tggattcaat gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt
    40081 ctttgggctt cacgccagtt gagacagcga cagatcgctc cttgttcatc atagtagatg
    40141 atttcttccg gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa tgattcgcca
    40201 ccttgggctt ttcctaaatg cagatttcct gcgatctttt cgatatcttc tccgccgcaa
    40261 ggtacgccaa aagacaagga aatactatta tagatatcta caagcggatt gattgggaaa
    40321 aattcccgtt cttgatttgc acgtttcagt aaagcttcaa tagaagaacg tgcacctttt
    40381 ttagtcttga atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat
    40441 gtttcttctg taaggtaatt tcttgcttct tttgctgcct cattcaataa ttgatgatac
    40501 gtttcttcac tttcttttgt tacgtggtta tcgataccat ttacggataa gatttcgatg
    40561 gttgcttcag gaaatacttc ccagaaattg gtatccacta ttactttttt catctgttca
    40621 cacctcttga ttagaataat tgcttgatga atagatagtc tgtttgggga tcatctccca
    40681 tataaaaaac atgttcgctg tatttgataa atcctttttt acgataaaaa gcgagcgcaa
    40741 catcgttgtg ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct tttgcttttt
    40801 caatagctgt atctagtaaa aatgtaccga taccttgatg tttaaatttt tttcgtacat
    40861 agattcgctc gatttctaaa aaatgatcag ccatttcctc agattgactc gttcctgtat
    40921 ttagtttcag gtaacccgca ggctcttgat cgatactcac taggaaaaaa tcggtttctt
    40981 tatcttttag ctcttctgta agtttttcta gtgcataagc ttgtgtcaag taatcagcta
    41041 aatgctccgg tgaattatct tttccaaatg tatctgtaaa tgtttcgata ctaatttgtt
    41101 gtagtaagtc tacatctgaa ggcgtcattt ttcgaatttc gtatttattc acggcttctt
    41161 cctttcaata gatcctttta ttgccttttt tgacaaaatt ccaatcttcg ctaacattct
    41221 tgcatacttt atttaaaaga tattccaatt gttttgcttc ttcgtcagaa agaccattta
    41281 atgcgactgc attggagtag ttgttttctc gaatgatgaa aggatagatt tctgctcctt
    41341 ttttagtcgg atagagtttc ttgatttttt tattttcttt atcttccaaa cgttcgatca
    41401 tcccattgga ttctaatttc ttgattgctc gggcagtcgt tgtacgatct actttgatca
    41461 tttctgctag tttttcttgg atgattccag gattttcgca aatacgtact aaataaagat
    41521 actgtcctct tgttaaatcg acttctttga attcgatatt gctaatagaa tctagcgctc
    41581 gggcaatcat tccgatgtct cgaaggattt cagccatttc atcccacctt tattttgttt
    41641 gtaagactag ttcacctctt tttgttgcaa atgcaactat tttcaataaa aaaatcaagt
    41701 tcttttttaa taactagtaa agtatattct tttcttcttt tacgaaaaag ctcaatcaca
    41761 atagatctgg tttttgtttt ctaaaataag taactgtttc ttcgttgttc gcttttctga
    41821 tagctttggc aactcttcta tactaaaaaa tcccatgttg gaagtttcat tattctcaat
    41881 aaacttggcc tcttcaccgt ggatcgcaca agcaaaaaca agtttataat actgaaaaag
    41941 ctgtggaata tctttttgtt tattcgtatc aaaaactgct cgcaactgag tagtttctac
    42001 tgttaatcct gtttcttcat agacttcttt acgcacattt tcttcaggag acaatcctat
    42061 ttcagcaaat ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga ttagtaggac
    42121 cttgccatct tttttgatat aggcacgaac atctactttt ggtgttggat gtccttctcc
    42181 aatcgttgtg agcgctttta tttcttcgaa agtttctttt cctatttcac tgatcagttc
    42241 caatgaaatc gtccggatct cctcaaaacg ctctttatca aatacatctt ttccataata
    42301 tagaccagct tgagaaagtg cgagtagtct tttgtattga gccaacttct tgttcattct
    42361 cttcccttct tttttagcaa atgtatgact atgtaatcaa gtatagaaga ctggtttaat
    42421 tacggcaaat aaatataatg aaatctgcaa taaataaaag aatgttaaaa aactcgagaa
    42481 caaaagacat tcgacttctc tggggctgtg aataaatggc gttcaaaagc ttgcatctgc
    42541 ggtaataagc cccaccaaac gaaaaatatg acgagctatt cttgtctggc tgttcttatt
    42601 aattgaggca ggacagcttt ttcacaacct ccccactaaa cggtgttctc ctaccaactc
    42661 ctcggtcaca agtcacaata ttcatcaaac atgagaagct gtttttttga aattgctcct
    42721 tgtgactcgg agctgaacga caggctcacg acctctcggt aaacagcgtt caaaagccaa
    42781 tttttcgata ttaagccatt tctttgttac ttattgtcat tgatttcttt ttctaattta
    42841 tccaatagtg gagtgtaatc cacttgagta tagtctgtta catcaaattc atatagtgtt
    42901 cctaactgga atttgtcata ttttcggtcg gcgatgatct ttttgaatgc ttctttcgta
    42961 atatgattgg tccctttcgt tcgatcttcc aatacgtcac catagtgata atgatccatg
    43021 atatagctca aatgacgatc tgattcacga tcacgtttgt accgacgttg ccataacact
    43081 tcaaagtctg ctgttaggcg aatcgtgatc acctgatatc cgtatctatc tgctaattct
    43141 tggagtcgtg ctttttgctt atcactaaaa ggatactcgg acacaatcac tttctttcct
    43201 gcttccatat acaaatcaag tgctttgtaa tagaaaggat aaactttcgt cctttcttgt
    43261 tccgcccgtt cttccaaatt gttaaacccg atggattctg catagtattc cttgatttca
    43321 tcgagcgtga tggcatacat atctggaaat cgttcccgta gtttattcat aagatatgtt
    43381 tttcctgtac caggactacc agccagaaga atgaaatact ttttcatctc atttatttcc
    43441 taagttaatt ggactttcca agttcataaa cagtccaatc ttatgcttga caatttcttt
    43501 tactgcttca tttgctggaa tcacattatg gcgaagagac ttgttgattt ctgtttcact
    43561 aaaggtttct ttagctactt tcgtccagtt gactaaaagc tccgttccaa cattgaattt
    43621 gcggatcccg ttattgatca attcaggata atcttcttct ttcacacctg ttcctccatg
    43681 gataactagg gggacatcaa ctactgcatc gatttctttc agcaagggaa tgtttacttc
    43741 tgtttttgat ttgaattgtc catgattcgt accgatagca atagctaatg catccacgcc
    43801 tgtagcttct acaaatttca ctgcgtcttc cggtcttgta tataccttgt cattttcagc
    43861 gacatggatt ccttcttctg taccgccgat tgttccaagt tctccttcta cggaaacacc
    43921 gtgcgcatgg gcatattcta ctactgcttt tgtcttcagg atattttctt tgaaaggcag
    43981 atgggaccca tcatacatga cagaagtata tccgctgtcg attgcttctt tgatatcatc
    44041 aaagtctcgg gcgtggtcta agtgcaagac tgcatcaacc atttcttctt ctgcaatagc
    44101 tgtcattaca ttgactaaca catgcatgcc gatatattta gcggtatcga cacttgtttg
    44161 aataatgatc ggcgaaccca tttcttttgc tgcgcctagc atagcaggaa gcatttctag
    44221 gttatgcgta ttaaaagctc caactgtaaa attcaactct tctgctgttt ttgttacttc
    44281 tttcaatgtt gtatacatct tttattgcca ccttatcttt ttatttgttt cctttgatcg
    44341 ttacttgttt tgcaatttgt cgcatctcat ccattttgcc catgtagtaa tcgatccctt
    44401 gatcatcacc agcacgagca gcatctgcac gcttctggtt atagaggctc atcagttttt
    44461 tatacccatc tccgatcgtc tgtttatttt ccaaactctt ttctaaatat tggatggctt
    44521 gatcgttcac atacaattca gaaaatttaa gtccaacttg ttcataaagt ttcgctaatt
    44581 tatgcttatc ttgggtagtg gaaatctctt gtttcaattg tgtaatctct gattgaatct
    44641 tttcggtcgc ttctggcgat agggcttttt cttcttttgc cagaactttt ttcggttctt
    44701 tttctttttt cttaaaaaat ccaaacatca ttttccctac tttcaattaa aatgctttca
    44761 aaactgggct taagatccag gcatataaga gtgctgcggc tactgtatct acatcggttg
    44821 ctgtagcatt gatgaatccc atcgaattaa agatcgttac tagcaaagcg ggcaataatg
    44881 taataaagaa accatgagca ataccgccaa tgatcgctcc tcggcgtccg ccaactgcgt
    44941 tgccaaatat ccctgctgtc cctccggcaa agaaattggt tagcatacca ggtaaaatca
    45001 tcgctaagcc gaacatgggc aaagtaaaca tggcaataat cgtaccaatc gttgtcgtaa
    45061 tgaaacctaa aatgacagca tttgggctat aaggaaagaa aactggacaa tctaaggcag
    45121 ggatcgcatc aggaactaat ttcatagcta tccctcgaaa tgccggcacg atttcaccta
    45181 acagtagacg tacaccagct agcaatacat acacacccac tacgaattgg atggcttgta
    45241 aaaaggcaaa catgatatag ttttgatctc ctgataattc agaagcgaat ggttcgcctg
    45301 caaataaaac agtgatgata tatagcggga ccatcacgac catgacagac aaataagtat
    45361 cttgcagaaa ttcgaaatgt gtcggcaact tgatatcttc gatcgacttt ttattttcgc
    45421 ctttctcacc aaaaagctta gcaacaccgg cttcaaataa gtacccgatc gtacaaaaat
    45481 gacctaaagc aatatcattt gatccagtga ttttacgaat gatcggttgc gctactgccg
    45541 gcatagcaat ggcgaaacaa gctccgataa aatcaccgac caagatcaat acgatccctc
    45601 gcagaccggc aaaatagcca aagactgtcg tcatcgttgc catccataaa attgcttgac
    45661 cagttaaaaa aatgtacttc cattttgtga aacgagccaa aataatattg aatacaaaaa
    45721 tggctaagaa cgtcagtgca atatctctac ctaaacccaa atcattcatt gcttgaccat
    45781 tgatagattc aatagaaggg atgatccctt gcatatgaaa tccttcagta aagattttcc
    45841 cgaaataagt taaacttcca acgatgatgc tcgaaccggc actcaacact tgaaatccta
    45901 ggagtgtctt taacgtgcca gagatgactt gtcctgctga tttcttctgc agtagtagac
    45961 ccaacatagc aatcaatgca atggtgatcg atgcctgagt caaaatattt tcgatgataa
    46021 aattgataac gcccatgttt ttaccctctt ttctttttgt atttagataa ctcccttatc
    46081 ttgcagaact ggggtgattt tttcttcaat ttcatttttt gaaacaatat ttttcaaata
    46141 gatcatcgcc gttttaggat caactgcgaa cttttcgaac tgcgaacgaa aattttctgc
    46201 tgtgatgatc acatctggtt tcatggaaac tgcggaagaa atatcacaat ggtccagttt
    46261 ggcttctact cctaatttgt tcaaaacatc ctcagcagac atctgtgctg caaaactact
    46321 tcctaaacct gcaccgcata cgaataaaat gttcaatttg ttcatctttg ttctcccccg
    46381 tatcatttat tatttctcaa ctactgaaaa aagcagttgt ttgaaatctt cgatatttgt
    46441 gcattcaatc aatcgatcca acttctcttc gttgttgatc aattcaatta gttctttcat
    46501 gatgttcaga tgcgagtagg agtctactgc tgccaaacaa aaaataatct ttactggatc
    46561 catttcagga ttaccgaaat cgattggttg ctcaatcgta gctacgctga ctcctaattt
    46621 attgacccca tcttctggtc gggcatgggc tagtgccatg tgtttgccaa tcacgatata
    46681 cggtccgtaa tcattcactg cacgaatcat cgcttcgaca tagcgctctt cgacaatgtt
    46741 ttctttaacc aatggtctag cagctagttc aatcgattcc tcccaagtag tggctttttc
    46801 tttgatcatg atattgctgt ccgtcaaaat atcttttaac ataggctgga tctccctttt
    46861 gttgatttct aaattgtttg tttcgaataa tttttctaac ttgccataga tgattcgatc
    46921 tactcggcca ccactttctt tcatgacatc tattatttga gtgaataact ccgtcgattc
    46981 atgactgtta ggctctaacc gctgtagttc ttcatttctt ttcaaaaagt cttctataac
    47041 caagatattc gtctcagtta aaatcggatc gacaactaaa agcggcttat ttcgataatt
    47101 caattgaaag gtgctgaaaa ccagattgac atccaattta tctactaatt cgatttcttt
    47161 tgaacttaaa actgcagtaa tctcgatctg aggaaacttt tctttcagat tctctgctaa
    47221 cagatttcca gtagccatcc catggttaca gacaacgact gatttataaa cataattcaa
    47281 gtctcgcttg attacactgg cgatcgtcga aaagtggatg acaagaaaag ctatctcgtc
    47341 ctccagtatc ttgccaccaa ttacttcttc gatcgtagga acaaaatgag tgactgcgcg
    47401 gtagattggg aaataatttg ttcggatgtt ttctttcaat ggattcatga cttggatatg
    47461 attcttgatt cttacaatca gtgcagccat gtgcttatag agattttcga ataaagcttc
    47521 ttcctttaga tggaaaggta cgtgtgtctc gttctctaca aattggatca attggatcgt
    47581 tagtaattga gcttgtaccc actcgattga attggagata tctctagcat tgaacgtctc
    47641 aatagtgaaa cgaagataat ttctttctac ttgcgggaca ttgtccaatt gaaattcctc
    47701 gatcatgtgg tcaataaatg cagaaaagct actgtctttt atttctgtat ttttccaatt
    47761 aatggcagag ataaattcat gtttcctgat tcgctggatc cagatcaatg taaaaagcag
    47821 gatttgtttt ttataaatat catcttcttg tttcaacaac ttattggaat agatctcctg
    47881 tatttttttg atttccacca aaggaaaata atggagaaag attttttgag ccggtgttaa
    47941 ttgtgcatct tgattcataa agaagtcggt cattcccaga ttctgattga taaaatcata
    48001 gatcatcgta cggatagatc gttctgaacc tttataaacc agtccttgct ttccataact
    48061 gacgatctct atcccataac tgaccagtgt tgctcgtaac cgtctcatat cttcatctaa
    48121 tgtactttta gagatttgaa attcttcttc ttttttgttt agatggatcg ggattcggct
    48181 gtaagcaacg gctaaaacta gatcaaaaat acgttcgcgc cgattcaaaa agccgtcttt
    48241 cacatctttt atttcagttt ctaatcgagc ttgcttttcc ttttccagct tcaacaaaaa
    48301 acctttgttg cgaattgtac ggatctccgg aagcttttta ctgctcaacc agatattcgc
    48361 ttcaagaact tcctgacgga tcgtccgctg actgacattg aactcacttg ctagatgttt
    48421 tgcttttact ggatattggg ctgtcatgat tctgtgaagt atttcaattg ttcttgtttt
    48481 catagaaatg atctccttct gatttcaagt atatggttcc gcttacataa taaaaagtag
    48541 atatttctgc cacttgaagt ggaaagaaag taagcaaagc aaacaaaaaa taagccatat
    48601 gctttttcat ctcctcaacg aaaaagacat acgcctatta ttacaaaaat tttgcccatc
    48661 tatacaaaaa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa caactaaact
    48721 aaaatagtgc ttcctatttt tcgtttggtt gttcttatta tcgcagatgc aagcttttaa
    48781 acaccattta gtggagaggt tgtgacagag atgttcagcc ttgagcgata agaagcgatt
    48841 ttgaaaaatc gcttcccata ttttacaaaa tcgtgcctta ttgccgatag gctatctctg
    48901 gcacaccgtt tattcagaaa aaggagctgc gacaaggctt ttgtcacaac tccgttcata
    48961 aatatttcct gtcaatccag cataatcggc tgaataaaat cggcttgcca tcttttttct
    49021 ttctcagaaa aatcaaaata ccatatctga gaaaaatact gtcgttgtgt cttacttcct
    49081 gagatcaatc gcccatcttt tcgatcgact gaataatcac agcatctgaa atcaattcgg
    49141 atggcaaaac tattttcgtg gatctgtcga taatttccta cttcctgaat agttattttt
    49201 ttagtatgat ttcttacacc gtctgctttg tttttttgca gaactttctg atgttcggca
    49261 aacagcttgt ctgtataaca gcttctggct ttttcaggag tttcttcttc ccaagcggat
    49321 tggatcttca tgaatatctt ttggatttcc agaaactgtg ctttcttttc tttagccgtt
    49381 cctgacatgc gggacaatat ctccgctttc agccttttct tttgtctttt attcttgacg
    49441 atagaaaaag cagaaacccc tcctactgac aaaacagcta atgcaagaaa tcccgtttgc
    49501 tcataactag aaggacctgt acctggacca taatacccat aagaatctct gccgtaagaa
    49561 cgactgccaa aataactgcc cccatatgaa cgaccaccaa atgaacctct gcccgaactt
    49621 cgaccgctag atgtacctcc accatgacca cccgcgacag cgaatccttg gggcggcaga
    49681 gcaaacaaac ataggcccac taacaaaatg attgctaaat aattgatttt tttcattgtc
    49741 ttcctcgtta tcttttatta caaaaaacta tttttatgat agccttgcta aaaggaagag
    49801 tcaataacca gatgcataaa ctgttttttt agcaattttg tcttatttag tcattgagta
    49861 acatcggaca aatgtatgtg tcggcagtcc ataattcctc tatcttcagc atgaacaagc
    49921 ttgcagacaa gagatctgcg cttcctccag gagagatttg gttttcctga caaaagtgca
    49981 acagtgctgt aaatggacga tttggtaaag cactttgact agcagcatga gcaagttttt
    50041 gcatttccac taacgttttc tgatcactgc gatgcaatac gcatgtgtca tctagcgaac
    50101 tcatcaaaac taacaacata tgcatttgtt tctgtacata tgaatcatta tctagctcct
    50161 tagctgcttg aattgcaagc ctgatatgcg gataccctaa tatcgcttct tcatatgcac
    50221 catttacgcc atatttttgc ttcaccctat gaccatgagt ggttttttta tgacgatatt
    50281 ttgtatcagg aaaagcagca agcttgctga catcatcaag tattttcgat aaagaaaaag
    50341 gctgttccat actgattttt tgtgcaatga cactgatcaa tagccccatt gcccaaatcg
    50401 ctcctttatg ggtattgatg ccatttgtag caatcagcat tgccttttcc gcttttcgcc
    50461 caatctcagc aattctttct cttaactcct gatcaatagc atgatgccat gctatttgag
    50521 ccatttcttc aaaaaaagga gttagcgtct tagcagactt aagaaataaa gcatagtcca
    50581 tatcggaatg tgcacccgca tccagtgcat ccactaatcc aggttttgga cttaatgtta
    50641 cttctgcgat caatgcttcc tctaccaacg cagcgagttg ctgagtcact gctttttttc
    50701 gttcgtcatt tatcatgaca ctcactcctt cttcctacta ttacttgctg aaaccttttt
    50761 tgatagctac ccaaatgata tgaagtaacc agcttaaagc aaatgcacaa cttgccgtca
    50821 ttagataagc tacaagaatc atgactcctg aaggtaactg cagccaacta tctttaaaca
    50881 ataataacag tacttggaaa acaaatacat tggctaaata cgtacgataa gcataaacag
    50941 acaaccattt gaacaatggc aattttggcg aatcgtttac aatcattttg gaagcgccta
    51001 tgaagacagc aaagatgatg actaaactgt ataaactttg gatcgtattc aagtaaggtg
    51061 cattcgcaaa gctcaagtct cctggatgtg cgagcaatga acggacagaa aagaacgcga
    51121 taactaaggc aacaggtaag aaaagataac gtactctgtc caaatattga tagacttttt
    51181 cgtgataaac aaatgcggct accccataaa tcatgaaact agcaacaaac cgatctaata
    51241 agtaccagct gtcatgataa ggcccagtca atacttgtgt ttgatagaac agataccatg
    51301 cgacatataa aatcgttgaa ccaatcaata ctggccatac atatttttta ttagcaaata
    51361 ctttgtaacc gagccaaaca aaaaacggca tgaacaattg gatctgcagc atcataacgg
    51421 tataccataa atgaggcgct ccatcacctg tgacatattt cagtaaaaag cttgcagtat
    51481 ccgtatacgg agtttgttgt tggactgctg gaaatagcca aagataagcc gtcgtccata
    51541 gaagatacgg aacgactagt tcaaaaaact tttctttcaa aaattcccaa taagacaaat
    51601 gctgacttgt ttttaccata ttataaacaa ttgcaaaaat aaacattggt gctgtgtatt
    51661 taacgatgac gtaaaaggta ccaagaaaag ctgtatttgt tgcgctgtgg ctgtttgcca
    51721 aagcaaagct caatatcgtt tgcatcataa cagccgttac agctgatact ttctggaagt
    51781 cacttacgtt gattttgatt tttttgtttt caaacatctc tttctctctc tttttcttta
    51841 ttaccaaagt tctttggtta atttaggtcc ttcgactgtt ttcaatagaa tttttttctc
    51901 tggctctctc aaatactctt ttagtgaaaa accgccttgc cgagtcacta cttgggtatc
    51961 gaattgctca aaaaaagcag gatgcgattg aattacctga attggcaatt caatcggtga
    52021 atttgcgtac agcagcaaat caaaatcact ggtttctttt actgccggaa tcccagtcga
    52081 taattcaaat cctaagctgc cgccgattcc ccatttacat cctgataata tgtttctagc
    52141 agcatgaaat tgctgataaa caggataatc catgattttc tcttgtctat acgattcttg
    52201 ttcgatgatg tcccacggat gaatgacttg tttgacggca tgttttgaga tttctgttga
    52261 ccatctctga ttgcgggcag gtccacgtaa accaatcggt agtatttctg cgtgatccgt
    52321 cgacgcacga cgaactgttc cgaacatctt ttcagttaga acgacccatt caggcaaggt
    52381 gaacccttct atttcatgtt tatcaaaaag gacaatatca tgtgcttgaa tcatttttta
    52441 tgcatcccat tcttcgtcca ttttggcacg gactttattt gtttcgacac gtccgaatgt
    52501 tttggctgtt tcgtttgtgt atcgatttgt cagcatcgtg tcatttgtat tgcggacatc
    52561 ttgaatggct tcattgattg cttcgatgac tttgctgaca ttttgttctg aagcagcttg
    52621 gtcggtgata tcttctagga aatgatagat cgctcctagt tttgtataat tattgatatc
    52681 gtaagcgata gctggaactt tttcagcagc tgcttcgatt tcagcaaccg aacgttttgt
    52741 cacacgtgca gcactttctt tagacatcgc ttggacggta atttctttat tgtttaaagc
    52801 aatcaagcgg cttgattgta atccatgtgc taaaaatgca cctgaaatag catttcctgc
    52861 aatcaatcca ataactggat gtccagcttg acggagtttt gcataagcag cagcactatt
    52921 agccaaagcc acatggatac cgatcaattc ttcgttatag ccgtatgctt ggcttggtac
    52981 atcgatcaca acgatgattg gccgtttcac ttttttgtct ttatcttctt cgtaaacgtg
    53041 atccaaaatt tgtcccatgc ggaaaccttc aactaaacca acttcaccgt ttcgtacacg
    53101 gtacatcggg tttttaggat caggtacgat tgatgtcacg atggcttctt ctatgaattt
    53161 acttgattta ccataataca cagtactaat agaagaagtt ggatttttca tgccagttag
    53221 tgccgcaaac caatcgtaac caacacttgc tgcagctggt tcatttcctt ctgctacttc
    53281 tggtaagtca attgattttg cttgatggtt agcaattgct tcattgtaat cgtcaatggc
    53341 cattggtttt gaaagatcta aagaatcaag taatgataaa taaaatgctg agctttcact
    53401 gcgatgcgtg tcttttcgtt cgtccattgc tgcgatgaca gcagctttga ttgcttctac
    53461 actatcagtc actaattcat caatgatttt tgattctacc cgttgacgag atcctaatgt
    53521 attccaaatc aagtctttgt cactagaatc aaattctcga acgcctgctt cttgttcaat
    53581 aacttctggt ccattcaaac cgactctcgc tttttttgtt gcgattaaat aactcattaa
    53641 tgctgaagta atcgacatac cgccaaatga tcctacacgt cctggaatca aaccgatgac
    53701 tggtacatat tgttttagag caatgattac atttccgatt tctgaaatag ataacagtcc
    53761 gtagtttgct tcttgcaaac gaacaccacc tgtatctagt acaatgactg gaaagatctc
    53821 atttcctttt ttattttctt ctaacacatg ttcaagtgca gcaacgattt ttgcaccgga
    53881 aacttcaccg ataccgccac cttggaattt tccttccata gcgattacta ctgcttgttt
    53941 gccattgatc gtgccacggc tgacaacgat cccgtcatca ctttcaggga cgattccttg
    54001 tgctggtaaa cttggcgcga tgatttgatc gaatggatca atcaattcac gtccattgtc
    54061 aagtagagcg ttcactcgtt ctctggcgtt taattctaca aaactatttt tcatctgaaa
    54121 gttcctccat tgcttgcgtc aatcgtaggt acacaactcc tggtgtagca ccgaagtcat
    54181 tgatcacgat atctgctgaa attggataac gatcaaaaaa tctcgttaat acattgttcc
    54241 atacttcgcc gaagccatcg cttcctgtac aaatttttac agtagattct gttttttctg
    54301 ttggaaacat caagatttcc aaatctcctg aaccgaccac tcctacatgg attttcttat
    54361 tgatcggctg tttcgttgga tatttataag ctaattgttc catggatctc tccccttttt
    54421 gtaaaacaga gcatacacgt tctgcttatt cgtctttctt cttccatctg ggtaaatggc
    54481 gacaaaaggt ttttctcgcc aattttatga ccaagttctg aatttcattg gtggttcata
    54541 caggccatct gaccattcga tcaaatcatc gatggtttgt gcagctaata atgaacgttt
    54601 cgcttcacta cgtttgatgc ctagatcttc tggtaatttc acgatgccgg ctgcacgcaa
    54661 gctatctagt gattctttcg tgctagtcat tccaagaggt gtaacaccgc caattgctgc
    54721 aattgctgct tgtcgttctt cttttgtttt tgctttgtac aaataagcaa ttccttcttc
    54781 tgtcacgata tgtgtagtat cttcgctata aatcatcact ggtgcattgg ctaagcctgc
    54841 ttgtttttga acagctagtg catctaattc ttcgacaaag actggttttt tatttgaacc
    54901 atatgtttca accatttgaa caactaattt ttggcctttc cctaatggat catcattgtc
    54961 gcgaagtgat tgccatgctg gtgtagaatg acgacgaccg ccaggattat gtcccatgtt
    55021 aggcgcaccg ccaaaaccag ataaacgtcc ttttgttacg gtagaagagt ttccttcgta
    55081 atctaattgc aaggttgaac cgataaacat atcgatggca tattgtcctg cagcttgtga
    55141 taatgttcgg ttactgcgca tcgtgccgtc tttaccgaca aaaaagacat ctgggcgagc
    55201 agcaatatat ttttccatac caacttcgcc accaaaacta tggatgcttt caacccaacc
    55261 tgattcgatg gctggaatca tcgttggatg tgggttcaat acccagttct tcgcgatttt
    55321 cccttttaac cctagtgatt caccataagt tggcagtaac aattcgatag ctgctgtatt
    55381 gaatccaata ccgtgattca aagattgtac gccgtgttct gcgtagattc ctttgattgc
    55441 catcattgcc attagaattt gcaattctgt gatgttttga ggatctcgtg tgaataaagc
    55501 ttctaattga taaggtttat ctgctttcac gacaacgtct acccagtcac cagggatatc
    55561 tacacgtggt aaagaatcga caacttcgtt gacttgaacg atcacgatac catctttgaa
    55621 agctgcagct tcgatgattg ttggtgtttc ttccgtattg aaacctgtgt acaagttgcc
    55681 gtctttatct gctttatctg cagctactaa aacaacattt ggaatcaaat cgatgaataa
    55741 gcgtccataa agttccaaat aagtatggat atcacctaat ttcattgtgc cgtcttcaat
    55801 catttgggcg acacgcaagc tttgtgcacc agcataagag aaatcgatct tgcttgcgat
    55861 tcctttttca aaaatattca aatgttctgg tcgtgagata ctagacatga tcaaatgcag
    55921 atcattcact ttttctgcat ctactttttc aagactttct gaaagaaaac ttgcttgttt
    55981 ttgattgtcg ccttctaata caactttgtc gccgggagca atcagttttt ccattacttc
    56041 tacgattttt tcagtttcta catacttgcc attgagcaag ttgctgattt tttccaatcg
    56101 ctgcgctttt ttttcgcgtt tagaagccca gttgcggttt ggtttttcca taattagtca
    56161 tctccatttt tgatatagaa acgtggcgtc atcaatacca tgttggtcta gattgatcgt
    56221 acggatccct ttatctacac caaattttgt atgaatcagc tttgtcaaag tattgccggg
    56281 gggaaattca atcgtcagat ccattcctaa ttcttttgcc aagtccatca tttggttcca
    56341 ctgcacaggg aaagctaggt tttccaacaa atctttacga atctcttctg cgtcctgagt
    56401 ggctcttcca tccgtatttt ttatatacaa acatctagca tcagatagat ggacagactg
    56461 catatacggc ttgaatttct tgaccgtatc attcatcaag agacagtgag atggcacagg
    56521 gactttcagc agcttcgctg tttgcgcttg gtgccgtttg gcttctgcca acgtaacgga
    56581 taaaccttcg atactcccag ataacgtatg ttgcatcgga caattttcat ttgacagata
    56641 aactggatgg tcaggatcaa atgtttttgc tacaatttcc tcagcttcgc ttctcgtcac
    56701 acctactact acgcccattg cataaccgct tggataagct tctttcatta agactgcgcg
    56761 gtgatagact agttccgctg cattctcaaa tctcaaagat cgacaagcaa cagctgcaca
    56821 aaaagcacca atcgaatgtc ctgctgcaat atcaggctga ataccgtttt tttccatttc
    56881 atctaaataa aatactgctt ttgtcagtaa tgctagctgg ataaaaaccg tatcttgata
    56941 gttttcttct ttatcaacta accgataccc cgtcacattt tctaaacgtt ccatgtacat
    57001 ttcagggata tccgctaaca tccctaactt ctgactgcct tgtccaggaa ataagaaacc
    57061 tgtactcatg agcctcgcct cctcagcact tactttttcc actgctatct tagtcaattt
    57121 ctgctataat aaaaaatgcc aaaaaaagaa aaaaccataa caaaaaggta tggatgggat
    57181 ggataattat gaaattgctt catctgcagt attttaaaaa agtattcgaa acaaaaaatg
    57241 ttacccaagc agccaaagaa ttatttattt ctcaacctgc cttgagccgt gcgatcaaac
    57301 atttggaaaa cgaattaggt gtccctttgt tttaccatag cggcagaaac attgagacga
    57361 ccgtttatgc agaagaattt tatccatatg cagtcaagac attagacaca ttagaagaag
    57421 gaatcaaatt attgtcttcg gtcaatgaga agatcgttac ttccgtcata ttgtatttag
    57481 aggtagcctc tgtttctatt ccgaacttgg tccgaatttt tatggaaaag catcctgata
    57541 tccaattaac gatcatgcaa catcatttac cagatgatat taccgatcct gtactgtaca
    57601 tcacttcgga gatgaagcct ggcatgacca atattcctat catgaaagaa cctgtttatg
    57661 tcgccattcc taagaaacat cctcttgcta aaaaagaaca tctgcaatta gaagatatcc
    57721 aacgcacccc catcttgatg ctgtcaaaga gcaacgcctt ccgtcataca ctggatgcag
    57781 cacttgaagc gaaaagtatc gaattgacga tcagttcgac gacagatgat cctgcgacat
    57841 tacgttctat tgtcaagcaa ggattagctg ttagcttttt cccaaaagtt tcttggtctt
    57901 acgataaaaa tgatcctttc gtcttgcggg aaatcaaaga tttgcccttg acacgaacaa
    57961 tttatttatc ttctagcttc acagaagatc atcctttgat caaaatgatt gcaaaaacga
    58021 tg
//
LOCUS       DAZKNO010000002        48853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000002 DAZKNO010000000
VERSION     DAZKNO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.002"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..1270
                     /locus_tag="ACG8MY_000054"
     CDS             50..1270
                     /locus_tag="ACG8MY_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0429293.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1267..3987
                     /locus_tag="ACG8MY_000055"
     CDS             1267..3987
                     /locus_tag="ACG8MY_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0429294.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3971..4915
                     /locus_tag="ACG8MY_000056"
     CDS             3971..4915
                     /locus_tag="ACG8MY_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0429295.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(4984..5994)
                     /locus_tag="ACG8MY_000057"
     CDS             complement(4984..5994)
                     /locus_tag="ACG8MY_000057"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0429296.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6174..6518)
                     /locus_tag="ACG8MY_000058"
     CDS             complement(6174..6518)
                     /locus_tag="ACG8MY_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0429297.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(6511..6945)
                     /locus_tag="ACG8MY_000059"
     CDS             complement(6511..6945)
                     /locus_tag="ACG8MY_000059"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0429298.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7083..7820
                     /locus_tag="ACG8MY_000060"
     CDS             7083..7820
                     /locus_tag="ACG8MY_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429299.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            7813..9024
                     /locus_tag="ACG8MY_000061"
     CDS             7813..9024
                     /locus_tag="ACG8MY_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429300.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9228..10010
                     /locus_tag="ACG8MY_000062"
     CDS             9228..10010
                     /locus_tag="ACG8MY_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0429301.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10080..10742
                     /gene="trmB"
                     /locus_tag="ACG8MY_000063"
     CDS             10080..10742
                     /gene="trmB"
                     /locus_tag="ACG8MY_000063"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0429302.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(10840..12594)
                     /gene="ade"
                     /locus_tag="ACG8MY_000064"
     CDS             complement(10840..12594)
                     /gene="ade"
                     /locus_tag="ACG8MY_000064"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0429303.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            12726..13655
                     /gene="comGA"
                     /locus_tag="ACG8MY_000065"
     CDS             12726..13655
                     /gene="comGA"
                     /locus_tag="ACG8MY_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0429304.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            13612..14661
                     /gene="comGB"
                     /locus_tag="ACG8MY_000066"
     CDS             13612..14661
                     /gene="comGB"
                     /locus_tag="ACG8MY_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0429305.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            14658..14936
                     /gene="comGC"
                     /locus_tag="ACG8MY_000067"
     CDS             14658..14936
                     /gene="comGC"
                     /locus_tag="ACG8MY_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0429306.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            14911..15381
                     /gene="comGD"
                     /locus_tag="ACG8MY_000068"
     CDS             14911..15381
                     /gene="comGD"
                     /locus_tag="ACG8MY_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0429307.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            15353..15700
                     /locus_tag="ACG8MY_000069"
     CDS             15353..15700
                     /locus_tag="ACG8MY_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429308.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            15663..16088
                     /gene="comGF"
                     /locus_tag="ACG8MY_000070"
     CDS             15663..16088
                     /gene="comGF"
                     /locus_tag="ACG8MY_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0429309.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            16060..16419
                     /gene="comGG"
                     /locus_tag="ACG8MY_000071"
     CDS             16060..16419
                     /gene="comGG"
                     /locus_tag="ACG8MY_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0429310.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            16591..17598
                     /locus_tag="ACG8MY_000072"
     CDS             16591..17598
                     /locus_tag="ACG8MY_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0429311.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            17623..18807
                     /locus_tag="ACG8MY_000073"
     CDS             17623..18807
                     /locus_tag="ACG8MY_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0429312.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19244..20827)
                     /gene="abc-f"
                     /locus_tag="ACG8MY_000074"
     CDS             complement(19244..20827)
                     /gene="abc-f"
                     /locus_tag="ACG8MY_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0429313.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(20932..21060)
                     /locus_tag="ACG8MY_000075"
     CDS             complement(20932..21060)
                     /locus_tag="ACG8MY_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429314.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(21405..21548)
                     /locus_tag="ACG8MY_000076"
     CDS             complement(21405..21548)
                     /locus_tag="ACG8MY_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0429315.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(21771..22250)
                     /locus_tag="ACG8MY_000077"
     CDS             complement(21771..22250)
                     /locus_tag="ACG8MY_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0429316.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22323..22607)
                     /locus_tag="ACG8MY_000078"
     CDS             complement(22323..22607)
                     /locus_tag="ACG8MY_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429317.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(22685..22879)
                     /gene="ssrS"
                     /locus_tag="ACG8MY_000079"
     ncRNA           complement(22685..22879)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG8MY_000079"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(22951..24237)
                     /locus_tag="ACG8MY_000080"
     CDS             complement(22951..24237)
                     /locus_tag="ACG8MY_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0429318.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24375..25046
                     /locus_tag="ACG8MY_000081"
     CDS             24375..25046
                     /locus_tag="ACG8MY_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0429319.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25012..25500
                     /locus_tag="ACG8MY_000082"
     CDS             25012..25500
                     /locus_tag="ACG8MY_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0429320.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            25513..26460
                     /gene="prmA"
                     /locus_tag="ACG8MY_000083"
     CDS             25513..26460
                     /gene="prmA"
                     /locus_tag="ACG8MY_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0429321.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            26461..27225
                     /locus_tag="ACG8MY_000084"
     CDS             26461..27225
                     /locus_tag="ACG8MY_000084"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0429322.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            27458..29671
                     /locus_tag="ACG8MY_000085"
     CDS             27458..29671
                     /locus_tag="ACG8MY_000085"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0429323.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            29682..30128
                     /gene="dtd"
                     /locus_tag="ACG8MY_000086"
     CDS             29682..30128
                     /gene="dtd"
                     /locus_tag="ACG8MY_000086"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0429324.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30299..31558
                     /locus_tag="ACG8MY_000087"
     CDS             30299..31558
                     /locus_tag="ACG8MY_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0429325.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(31647..32594)
                     /locus_tag="ACG8MY_000088"
     CDS             complement(31647..32594)
                     /locus_tag="ACG8MY_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0429326.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            32988..34280
                     /gene="hisS"
                     /locus_tag="ACG8MY_000089"
     CDS             32988..34280
                     /gene="hisS"
                     /locus_tag="ACG8MY_000089"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0429327.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34295..36064
                     /gene="aspS"
                     /locus_tag="ACG8MY_000090"
     CDS             34295..36064
                     /gene="aspS"
                     /locus_tag="ACG8MY_000090"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0429328.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36173..37048
                     /locus_tag="ACG8MY_000091"
     CDS             36173..37048
                     /locus_tag="ACG8MY_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0429329.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37314..39197
                     /locus_tag="ACG8MY_000092"
     CDS             37314..39197
                     /locus_tag="ACG8MY_000092"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0429330.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39272..39556)
                     /locus_tag="ACG8MY_000093"
     CDS             complement(39272..39556)
                     /locus_tag="ACG8MY_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0429331.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(39546..40280)
                     /locus_tag="ACG8MY_000094"
     CDS             complement(39546..40280)
                     /locus_tag="ACG8MY_000094"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0429332.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            40409..41032
                     /locus_tag="ACG8MY_000095"
     CDS             40409..41032
                     /locus_tag="ACG8MY_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0429333.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41224..42345
                     /locus_tag="ACG8MY_000096"
     CDS             41224..42345
                     /locus_tag="ACG8MY_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0429334.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42352..45480
                     /locus_tag="ACG8MY_000097"
     CDS             42352..45480
                     /locus_tag="ACG8MY_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0429335.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            45499..48693
                     /locus_tag="ACG8MY_000098"
     CDS             45499..48693
                     /locus_tag="ACG8MY_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0429336.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLQELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
BASE COUNT        15962 a         8607 c        10237 g        14047 t
ORIGIN      
        1 aaaaacattg aaaatggtat gataaaagaa aaaaaggagg aaatttttaa tggtgaaatt
       61 tatccatgcg gcagatttac atatggatcg gtcatttgaa ggactgacaa ctcttgataa
      121 aactgtacag gaaaaactat tgaagacgaa tctgaccgtt ttatcaaata tcattgaaca
      181 ggcaataata aataacgttg attttgtttt acttgcagga gataattttc atcaaaatcg
      241 tccttcatta aaaatacaga aacatttttc agaacagatg aaacggttag aaaagaacca
      301 tatacctgta tttataatat tcggaaatca tgatttctat caaaaagagc ggtattggtt
      361 ctcctttcct aaaaatgtcc atttatttac atccgaaacg gtagagacaa aaaaaataac
      421 gataaaaagt ggagaaactg tctctctcag tggatttagt tatcgtcaac cgtggattca
      481 aaaagacaaa gtgatggaat ttccttctag agaactcaca gattaccata ttgggttgta
      541 ccacggggaa cctggcgtct ctcaaaaagg aaactatgct cctttccaac cctcaaagat
      601 gcaagaaaag ggatacgatt attgggcttt agggcatatc catgtgccaa cggtcttaaa
      661 tgaaaagcaa acaattgttt atccaggtgc gcctcaaggt catactaaaa aggaacaagc
      721 ttctacatct attctactag ttgaattaag taaaggaagt tgccaaatac atccgattaa
      781 agtagcagag gtctattgga agacaaagga aatatcattg aggaaagcaa gaacgacaaa
      841 agaggtcatt gttcacattc gtcagcagct ttcaaaagta gaggatggat ttagtttaat
      901 tgagataaaa ttaaaagatt atgaccatct caatacagat gttttggaac ggatacaatc
      961 aggagagcta ttagactatt tgttagagga attctcagat aggataaatg atttctttat
     1021 ttggcgtatt tctctcatag agaatacttt ttatacgaag accccacttg ctgcttctgc
     1081 taaattgatg gaacagttat ttcaatacta tcagacacct caagatttcc aacaaatttt
     1141 aaatgaagta tatagtcatc aagaagcggc tagaatattg agcgaactgc cggaatatca
     1201 agaagaaaca cttgaaaaag ccaagcagct gcttaatcaa gattttttat ttgaggagga
     1261 tcaggaatga agattttaag gatcgagatt gctgcttttg gaaaatggcg tcaaaaaagt
     1321 tttgactttt attcaggcaa tcagctgatc tatgggggaa acgaagcagg aaaatcaacg
     1381 atttatcaat ttattcaagc aatattattt ggtttccctt caaaaggccg taaaaaaaag
     1441 gattatacac caaaagacgg ctcagcctat ggcggcaaaa tatggcttaa acatcctgta
     1501 tatggtgaat ttgctgtcga gcgctacaag cagcaaaatc gcggaaaatc gaaggtgtgg
     1561 ctgggggatc aagtgggaag tgatgagtta ctagaaaaac tgatcgcacc tttgacgaaa
     1621 gagttattcc agcaggtatt tactttccag caagagcagt tgatggaact agatcatttg
     1681 agagaaaaag agctacatga tgcgctgatt tctttaggca tcacgggcag ttctcaagta
     1741 ttccaaaagc ggcaagaata ttatcagaaa gcacaacaat tattcaagaa aaaaggccaa
     1801 aaattactaa tcaatcaaaa attaaatgaa tggaaagagc tgcaagaaaa aattcatcaa
     1861 aaacaagaac aggaagctta ttttggcaag ttgatccaac aagaacagga atacgagtcg
     1921 caacagcagc agttattggc acaattaaaa gaggcgaaca aacaattttc attagccaga
     1981 cagcaagaac ttaatttttc tttatatgaa gaatggcaat ctctcaaaaa aaacagcact
     2041 acaggtcttc ctgatgagca acttctttta gatctggagt ctttctccaa acggtatcag
     2101 caaacaaatg agcgtttgga agaattagaa gcaacactgg aaaagcatag cgggatggat
     2161 cagcagtcag ccagatatta tttttattta gagcaggagc aggaactaaa agatctccag
     2221 caacttcaat ttacagcgga acaattaaac gaagaatggc aaagattaca aattgagact
     2281 agagaaacaa attcagaatt gaagattttg gaacaaaggt ggcattggca gaaaaatccg
     2341 ttaccggaag aatttttata tgaagaagaa tggaagcatc ttctggctga agaaagaagg
     2401 attgctgatc tagaaagcca gatttctgtg cgtctccaaa tagctaaaga acagcaagag
     2461 atgattgaag cagaagtgaa tgacttggaa gagacctatc cagcattatt acaaaatcag
     2521 tcagagatag caccagtctc ttcatctaaa ccgtatatgc tgatggctgg cggagcaata
     2581 atcattgcag gtatatttgt accaatgccc ttgaaagtga tccttttact aattggtgca
     2641 ggcatgattg gctatttctt ctacaaaagg caaccaaaaa ttaaaactaa aaatgatttc
     2701 tctgagataa aaggaatgtg gcaagaaaaa ctaggacaac ttgatctagc cttagctcaa
     2761 ttagctgaag cagaagaaaa acaaaagaga actggagaag aaaaacagat tcttcagcaa
     2821 gaagtatatc gttttgcgga tcatcggcac ttaggaaaga tggatacgct caaactcatg
     2881 aaagaatatg gacaggaagt catagattat cagaaattat atggcgtatt tgagcggaaa
     2941 cataaaagag aacaggaaat acaagaaaaa ctacgacaaa tcgaccaaaa atttgagttt
     3001 gttaaagaat ggttaccgat acaagataag acaatttcag aaaaaatggt attgctttcc
     3061 cgttttatac aagagatgga acagatgaaa tttgccagaa gctatcagca aaatacttta
     3121 ttgaagcaag aaatcaatca attgaaaaag aaacaaaaag tatggataca agaatacagc
     3181 gaacaattga ctcaagcagg cttatcttat ccttcggaag tacctgtctt tttacaaaaa
     3241 gccaaacaag ccgaacagca gatgaaaaga tacaatgaat tatcggaaat gcttactcca
     3301 ctatttcctg aagagctgac aaaagagaaa ttatctgtaa gactgcgcca actgaaagaa
     3361 aagcaatccc aactccagca agaagaaaaa gaagcaagtg aaaatcggca acgactccaa
     3421 cttcaagtag aagagttgca aagagacggc acattggacg aactttatca acaagctagc
     3481 cggcaaaaag caaaattaca ggaattactg gaggaatggg gagcacttga aatcgccggg
     3541 acattcttag gtgatctttc aacagagatg agtgaaagac aattacccca gttgttaaga
     3601 gcagcaggtg attatttagg tattttaaca aaaggaaatt atcagaagat attactgaaa
     3661 aatgatacac tgactttaac agacggatat acagaatttc ccatctatga gttatctaca
     3721 ggtacgaaag atcagctgat tatggcgatg agatttgctt acctctcatt agaagctaac
     3781 cgttcgatat gcccgatcat cattgatgat ggctggcttc attatgatca tcagcgaaag
     3841 tatcaattag ctgaactttt agctaaattt ggagaaaagt atcaaataat ttgtttttct
     3901 tctgataaag aaatggtaag ctattaccat gagatgaacc aaatcgtaaa acaattagaa
     3961 ggagcaaata atgaaaaaaa ttcgtgattt agtaatcgat gagcagtttg aacaatttgt
     4021 cctgatcaaa agtgcagatg taagaatcgc taaaaacgga aaaaaattta ttgcttttac
     4081 tttccaagat acttcaggta ctatcgatgg taaatactgg gatgcttcgg aggaggaaat
     4141 caataaattt acagccggca cagtcgtttt tctaaacggt aaaagagagg tttatcaagg
     4201 aaaccctcaa gtaaagattc ttcatatgcg cttaacaagg gtagatgagc caggagagcc
     4261 tagtttatac atggaaagag cccctttgaa gaaagaagaa atggaagaag aaatcaatca
     4321 attgttgttt gagatcacca atgcgcattg gaatcgtatt gttcgctatc tacttaataa
     4381 atatcaaaaa caattttttg agtttccggc agcaaaaaga aaccatcatg catttgccag
     4441 tggattagct tatcatactt tgacaatgct tcacttaggg aaatcaatcg ccgatgaata
     4501 tcctgagctg aatcgagcat tgttgtatgc tggtatcatt ttgcatgatt taggaaaggt
     4561 gattgaactt tccggtccta tgtcgacaga atatacgctt gcaggaaatt tgatcggtca
     4621 tctggtattg attgacgaag aaataactaa agcctgtctt gcgttgaaaa tcagcgaagc
     4681 tgacgaagat gttctcgtat tacgtcatat ggttttagca caccatggct tattagaata
     4741 tggctcacct gtacgtccaa gaataatgga agcagaaata ttacatcaaa tcgacaatat
     4801 cgatgcttcg atgcagatga tgttgacttc gattcgtcaa acagaaccag gaaaatacac
     4861 cgatcggatt ttcggtatgg acaaccgaag tttctatgta ccaaaagatt tatagcaaaa
     4921 atttattttt ttcattcaaa acaaaaaaag cgagacataa gtcttcgctt tttttgtttt
     4981 cttttatttt gatgaatcag ttgtttcttc agttgaagat tcttttgttg aatcagttga
     5041 ttttgtgtca gaactctttg ttgaagctgt agtttcactt gagtctttcg tacttgatga
     5101 agttgttgtg aatgcactca acacattttc aaaggcatca tctttgattt ttacatttgc
     5161 atcttttaat tcttcaccga ttactttagt tgtgaatgca ttgtcactta gtttagtttc
     5221 agtagcgata tcttttagtt gatctttgta tttatccatg tcattgcctt tattttggtt
     5281 tttcaccatt ttgacaacgt agtattctgt cgcataagaa gtaggatttg ttgtagtaat
     5341 aacatcggag atctcaccgt ctttcaattt gaaagctgct tcttttactt ctgcaggaat
     5401 agttgttgtc gttgagtcga atttaacttt accgccatct tcttttgtct ctgtatcagt
     5461 tgatttgtct ttggctaatt tagagaaatc atctccatca tccgctgatt ttttcacatc
     5521 ttttgcttca tcttcacttg ataatttgat gatttgtgct tctacttctg gatggaaaga
     5581 tttccaagca gttttcaaat cgtcatctgt aatatctacg tgagctttca agccagcttc
     5641 aaaagctaaa ttgttacgaa tatattcttt ataagtatct ttcgtatatc cagcagcttc
     5701 taattgtgat tcaaaagtat cacctaatga tttcgcttgt ttgtcgtatt ctgcatccac
     5761 ttgcttatcg gttactttat cgccatattt gttattgaat actttataaa tgatcatccg
     5821 ttgtactaag gattggttcg atgattcaag ttttgcttca tcatagaaat cagatacagt
     5881 gatcgttccg cctttcatcg ttgcgatatc tttattagta tcgcctgaac aagctgctag
     5941 agttaaaaca gccaacgcac tagtagctgc caaaataatt gatttttttt tcattaaaat
     6001 ttgcactcct gtaattagtt attattttta tatattaact ataccatatc ccatttcctt
     6061 taaaaacgtt gtcgtggtag tttcaaacaa aattcaaaat ttcatcacat tcctaggaaa
     6121 tacgcaaact tttctcactt atatagttaa atctgttgag aaaataatca ttattatatt
     6181 tgttcaggat actctggcag atcagctttt agtttttcta cttgttcatt gatttgtgtg
     6241 atacgtggtt cggcttggaa tttaaaatct tcgatatctt ttttgattgc ttcttggaaa
     6301 gcaggaatca cagattcagc tgtttctttt gtttcgatca atgcgctttt aaaatgattc
     6361 aggctgttat ttaaatcatt tgtcagagtt gttgcttctt ccaattcttc gatcagtttc
     6421 tttcttgttt cattcccgct cctgggcgcc aataacagac ctccgacagt gccagcagca
     6481 gctccaaata acagtccttt gaaaaagttt ttaatcatcg caggacacct gctttacgat
     6541 tgtttcttga atagctttta gttcttccga tccataacta tcttgatgat tgacaaagtg
     6601 aatagaaaaa tcatcctctt tgctgtagcg agggactaaa tggatatgtg aatgaaaaac
     6661 agattgatag gctagttcac ggttgttatt caagatatta agacctttca tttctggaaa
     6721 agctttttcc aatgcttgag caatcttagg cacacgcgaa aaaacctctc ctgcaagttc
     6781 gggatcatat tcaaaaatat ctgctacatg acgtttaggc acaactaatg tgtgtccttt
     6841 cgttgcttga gagatgtcta aaaaagcata gaccttgtcg tcttcatata ttttatagct
     6901 tgggatttct tgattgatga ttttgcaaaa aatacagttt tccatataaa acatcctttc
     6961 tataaagaat aaatacttca gcttaacttt accataagtt aaagctattg taaggaaaaa
     7021 aacaaaatac cgtcattttc ttcgtggtat aataaataaa aataaatggc ggaggaaaaa
     7081 gaatgagttt aattatcgat catgtcacag gggggtacgg acatctccct gtgttaaaag
     7141 atatcagttt cgatgtacag cctggagaga tagtagggtt gattggactg aacggagcag
     7201 ggaaaagtac gaccatcaag cacattatcg gtctgctgga agcttcaaaa ggaacgatag
     7261 aaatcaatca tctatcctta aagaaaaata cggaagaata tcgcaaacaa attgggtata
     7321 ttccagagac tccttcttta tatgaagaat tgacattgag agaacatatc gaagtcactg
     7381 cgatggccta cgatatacct atggatatag caatgaaaag agcagaaaag ttgttggaga
     7441 cttttcggtt atctaatcgt cttgactggt tcccagctaa tttttcaaag ggaatgaaac
     7501 agaaagtgat gatcttatgc gcctttttga tcgagccttc tttatatatc atcgacgaac
     7561 catttttagg cttggatcca ctagctatca atgcattgct tgaattgatg gtagaaatga
     7621 aaaaagaagg agccggtatt ttgatgtcta cgcatatttt agctactgca gaaaaatatt
     7681 gtgatcgttt tgtggtctta cataacggag agatacgagc agacggttct cttgagatgt
     7741 tgagaaaaga atttcagctg ccggattctt ctttggacga aatctatatt gctttaacaa
     7801 aggagcaggc acatgagtag tttttttcaa aaacgtttag caagacatca aaagaaaatg
     7861 atgaaatata tgcgttatgt attgaacgat cattttgttt tagtttgtct tttcttgatt
     7921 ggcggtgtgg gattatatta ttctaattgg ttgaaaaccc tgcagccacc gtttgcaatt
     7981 ggagggctca taatctctgt attttggatg ctctgtttat tcgtgggtaa gatggctact
     8041 tttgcagagg cagcagatat tgttttttta cttcctaaag aaaaagaaat gcaaacctat
     8101 ttggaacgag cttttcgcta ttcttgcatt tttccgttga tctttttgat attggcatgc
     8161 ggattggcca tgccattagt ggtggttagt acggagcaat ccttttcgat gtttttcgtc
     8221 tatctgatca tattatggag tttgaaattc agtcatctgc aaatcaagag agcagaattg
     8281 tttcgactag atcaacaagt tattcgttat tggaaattag gctggttttt ttcttctctg
     8341 atctttttgt ttctatcatt ttattggcta atatgggcag gaatagcagg tgcattagta
     8401 caggcaagtc tatattactg gtttttgtgg aagcaacaag aaaaaggatt ggattgggag
     8461 aaaatggtgc atgtagaaga acataggttg catcagatct atcaatttat caatttattc
     8521 acagatgttc ctgaaattac ggcaaagatc aaaagaagac gctatttgga tcctatattg
     8581 actcatatta aaagagaatc aaaaaacact tatctttact tgtttgcaag aagattggca
     8641 agaggttctg attttagtgg tctttatttc cgattagtaa ttataggtgg gttattgatt
     8701 gccggagtag tcgactttta ttttagtctg attatcggtt tcttattttt atatttgatc
     8761 ggtttccaac tgttaccttt agcgaaccag ttccgctata tggttctcgt tcagctttat
     8821 ccaatccata agaatcagaa gaagcaggcg attcagtatc tactcacatg ggtattaatt
     8881 atcgcgtcgg ttatttttgg tttgattgct gcagtcgtat tagatgggca gcagcgatgg
     8941 gttccgctag cggcttattt gattgttact atgctctttg ttaaactata tgtaccaact
     9001 cgtttgaaga agatgtcaga ctgaaaacaa agctgtgtag ggtaaaaatg agaatgaaaa
     9061 atagagcgtt gacagtaaga atgctgttcg ttctattttt tttgagaaaa gctggttttt
     9121 tgaatggcaa ttaaatgaaa aagaacgtat atgtgcttga cgtgtagtgt tcaggcgact
     9181 agaataataa gtactgcaaa acaaagagta agaggacggg agaatgcatg atggagtttc
     9241 agttagacca agagtggcgg ttacgtccca tcaaaggcga cacaggaaaa acatacatag
     9301 gcatgcgtga tgaggacaaa gtctttatta aacgaaacac gactccgatg ttggctgctc
     9361 tttcaaggga aggaattaca ccaaaacttg tttggacaaa acgaacgggg aatggcgata
     9421 ctttgacagc acaagagtgg ctggaaggtc gcttgctgac tcctaatgag attggtcaaa
     9481 gaaacgacgt gattgatgtt ttgtaccatt tacatcattc aaattctctt aaaaccatgc
     9541 tgacccgtat cggtggtcgt gtcatgacgc cggagaagat gctgcaaaaa tatgaaaaag
     9601 aattaccgga agaactccag aaaaatcaat ttctgcaaat tgtccatcgt tttcttcgtg
     9661 agcagatccc gtcaatagat aaggaaaact tatcagtagt acacggagat gtaaaccatc
     9721 gtaattggtt agtatgtaaa aattatttat atctagtcga ttgggattca atcatgtttg
     9781 cagaccctgc ggtggatatt gcaacgattt taggaaatta tgtgccactg tctagctgga
     9841 atcagtggct gatcagttat ggcatacgcc cgactgacga aatgcttgaa aaatttcatt
     9901 ggtatgcggc catgaatttg ctccaagaaa tcaccagata ttgcaaacgt gaagatcatc
     9961 gacggatgaa cgaagaaatt ttacaattaa aacgaatttt cagtggctga ggtttggccc
    10021 aaactctgcg gagatttggc cagacctttt taattagagg aaaggaatgg agataaataa
    10081 tgcgtgtccg aaatcgtccg ggagcagcgg aaatgttagc ggctcacccg aattttgtaa
    10141 ttagtgatcc aacattgtgg aagggaaaat ggaatgaatt gtttgaaaac gatcatccaa
    10201 tccacattga gattggtatg gggaaaggcc agttcatcac tggaatggca aaagcccatc
    10261 ctgaaatcaa ttatattgga gtagaaatgc aggtcagtgt tgtttcgatt gcactagaca
    10321 agttgatcga acaaccgctt cctaacctga aacttctaca tgtggatgga tcagcgttga
    10381 cagagtattt cgctgacagc gaagtagatc aaatctatct gaatttttcc gatccatggc
    10441 caaaaaaacg tcatgaaaaa cggcgattga catataagac ttttcttgct gtagatgaac
    10501 agattcttcg tccaaatgga gaaatccatt tcaaaacaga taatcaagga ttgtttgagt
    10561 attctctggc aagtttttct caatatggca tgatcctcaa acaggtatgg ttagatttac
    10621 atcaaagtca atttgaaggc aatattatga cggagtatga agaaaaattt tcttcaaaag
    10681 gtcaacgtat ttatcgagta gaagctcaat ttcaagataa gaccaaaaag aaaagtctgt
    10741 gaaactaact ttctgctttc ataaaaacat taatatccat aaataaatcc cagaaaatga
    10801 acattacaga tgtaatgtat tttctgggat ttatttgatt taaaatgtat ttatttcatc
    10861 ggtttttcca caaggtggaa tcaattggaa gcgagaaaat gaaaataatc ctttgtcggt
    10921 gatcttcaat tcgggtatga cagataatgt taagaaagaa agtgtcaaaa aaggatcaaa
    10981 ggttttagct gctcctagtt gatgtgcttt agttattaat tcattcaact gttcatagac
    11041 gatcgaaaaa ggttcctgtg tcatgattcc gcctatagga agagaaacac aagctatttc
    11101 ctgctgtttg ttgacaacgg tcatcccgcc acctttttga atcaaaagat tagcagcata
    11161 cagcatatcc tcgtcgtttg tcccaacgac aactagatta tgcgagtcat gggcaatcgt
    11221 tgtcgctatc gctccttgtt tcagctgaaa tcctttcact atgcctaatc caatgtttcc
    11281 cgtttgatgg tgacgctcga tcaccgcaat ctttaattgg tctttttcga ttgaaggaat
    11341 gaatcgttcg ccttgtcggt ccactttttc taccaaatct ttagtcacta gactattcgg
    11401 gataatttcg atcacatggg cttgtgaaga agaaagaagc agctctaacg aatgtggatt
    11461 taatggttga gcgtttagtt ttggcagaca acacgtatat gaatttgaat gatcagcaaa
    11521 taaatggtct tggagttgcc cagattgaac aactaatgtt cccttataga aaacttcatg
    11581 aattggcagc ttggtaagat cgtctgttaa gaagaaatca gcttgatacc caggggcaat
    11641 cgcacctagt gttttcaaat caaaacattc tgctgcattc aatgttgcca tctgaatggc
    11701 ttgtaatgga tcgattccaa gtgcaatagc ttgacggacg atatgatcga ttgatccttc
    11761 ttttactaaa tcattgatca gtttatcatc ggtgacaaag aagcatcttc ttgaattttc
    11821 aggtgtgatc gccggtagta gcgatgccag atcttttgca acggtccctt ctcgaaccat
    11881 taaatacata ccaagatcca aacgttcttt agcttctttt gcagttgtag cttcatgatc
    11941 cgtccgaata cctgctgcag gataaacatt taactcttcc atgccgatac cagctgcatg
    12001 cccgtcaatt tttttcttat gctggtgcat caattggatc ttttctagga tgtctgtttc
    12061 attatttgct actgcttgat agttcatgac ttcagctaag cctaaaactt cgggtttttg
    12121 taaaaaaggg gctaattttt ttgcatttaa atttgcacca ttggtctcaa aaggggttac
    12181 aggtacacaa gaaggtagca taaaaaagat attcatgagt gtttgtcttg catcttctat
    12241 cataaactca agacctgtac tcccagcaat gtttcctatt tcatggggat ctgtcactac
    12301 agtagtaact ccatgaagca aggatacttt tgaaaattcg cttggtgata aaagtgagct
    12361 ttcgatatgc acatggccat cgatgaatcc tggcactaga tattgcccat tagcattatg
    12421 tacgcttttt ccttcgtatt caccgattcc tacgatcatt ccttcgcaga ttgctacatc
    12481 tttttccatg atttctttcg taaagacgtt gacgatctta gcatgcttga tcactaggtc
    12541 agcaggcttt tttttacttg ctacttcgat atatttcctt aatgttgttt ccaatggagt
    12601 gcctcccaag ataaattttt ataagtataa cataaattcg gtttttgcca atattttttt
    12661 gtaaattcat tttatttgaa acaaaaagat caaaaagacg tatgtttttt gtggagggga
    12721 gaataatgga aataaaacga ttatcggatc aaatgataag tgaaagtttt gccaaacatg
    12781 cccaagacgt atatatattg ccagcttcag aagtatttta catctatttt cgctatggaa
    12841 aaaaacgaac attctggaag aaagtctctt cggcagatgg acagcaactg atctcaagat
    12901 ttaagtattt gggacagatg gatatcggag aaaaaagaaa agctcagtta ggtgcattta
    12961 cttattcttt agaaaaacag gaagttcgtc tgcggttgtc ttctgtagga gattaccttc
    13021 aacgagagag tttagtgatt cggctattat acggtacttt tgaaacaccc catcgttgct
    13081 ttagttctcg agattttgca actattttac agaaaaccgt cagacgtgga ttataccttt
    13141 tcagcggacc agtcggatca ggcaagactt ctctaatgta tcggttggct ttggaagaaa
    13201 aattgcaagt gattactatc gaagatcctg tagagataga agaaccgttt tttctgcagc
    13261 tacagatcaa tccgaaaata gcacagactt atgatacact tttgagactc gcattacggc
    13321 atcgtccaga cttgctaatc attggcgaga taagagatga atcaactgca catgctgcgg
    13381 taagagcagc actaacaggg caccgggttt ttgcaaccgt acatgcacgc aatcttgaag
    13441 gtacattggt cagaatccat gaactgacaa agaaaaaaga agagctatct gaatgtttag
    13501 ctggtgtcat ttatcaagaa cttttggaag atgccaatca ccatccttct gttttatgga
    13561 gttatcaatt tatggaggaa gaatcagtaa aaaaatcatg ggaggaaagt tatgaagaag
    13621 ctcaggaaaa tatttggcag tcagaactat tctaaaggat taacaagaag ccagcaaaac
    13681 cagtttgcac aaaccatggg agaactgctt ttatctggtt ttagtctgca agaatccctt
    13741 tatttattac tcacgatccg ttcgtttcca cctgttattt tggcaaatat ccaacatcag
    13801 ctaaaggaag ggcaagcgtt tagtgaggta ctttcgcaaa taggatatgg agcagagcaa
    13861 ctggtacaga tcgagttagc tgaacgtcat gggaatctgc cgacaacttt gttggaaatt
    13921 gccaaacagt ttcggcttgt tgaaggattt cgcaatgatt tgaaaaagct gcttgcatat
    13981 ccgatgctat tgctcacctt tttgctaggt atcttgttta gtatgcggtt gttcgttttg
    14041 cctcagctta ttggttcagg aatggtcatg caagagcatt ggggtattca gctgatacaa
    14101 gtctttcatt ggtatgttct actattcatc ctagcggtta ctctattggc tttactagta
    14161 aggtatcgtc tgatgaagtt gtctcctgtt gatcagagtg aatggataag cagacaatta
    14221 ttgattggac cgatatattc cttgtatcaa tcggcttatt tgtcactaga gattggaaag
    14281 ctttttcatg aaggtttcga attaaggcaa atcatagtct gcttaaagga aacgaaacaa
    14341 gaaagtctga ttcagcatct tgcctctaag ttgattcaag gattggaggc aggtatgtca
    14401 ttggctgagc agttccagaa ttatcgtttt ctatcagaag aattcagtaa aattgtttta
    14461 cagggagagg caaagggaag tttaggtaaa gaactgattt tttacagcga atggacaaga
    14521 aaacagcttt tcaaacgtct tcatcactgg atgcatttta ttcagccaat catttttttg
    14581 ggcgtagcgg ctttgatttt attagtttat gccgctgttt tattacctgt ttacggaagc
    14641 atagaggagg ttttgccatg aaaaagagaa aaatatctta ttatagcggt tttacattga
    14701 tcgaaatgct aatcgtctta ctgatcatca gtgtattagt gttgttgttc gtccctaatt
    14761 tgtcgcgtta taggaatcat gtagaccaag aaagtcgtga agcgatcatc cagttggtag
    14821 acacacaaaa agagctctat gcgcttcaaa ataatggaag agttcctact gtggaggaat
    14881 tgttgaatga aggctatatc aaacgagaac atgcagaaat ctatcagcgg ccttgaaggt
    14941 aaggttttag catcaggata tacgttgttt gaatcactct tactgatttt attacttaca
    15001 acgatccttt cttttccagt tctcgcattt tctgcatgga agaatgaatt ggcagtctat
    15061 caatttttcg gccaatttga aaaacgaatc tattctacgc aaaaaatcgc tattgtgaat
    15121 cagatccaaa caggctttta ttggaatagt gaagagaatc aaatcatttt tcatgttccg
    15181 aatccagata aaataaattg ggaagtatta gatattcctg atgagattac cttgaaacgc
    15241 catgcttcta ttacttttgc tgctagaacc gggaatgaaa gttcactaaa agcttatcaa
    15301 ttttattggg aagaaaaaga gcggacgatt acgtatcaat tccagatagg gagtggtcgc
    15361 tatacaaaaa gaattgaata aaacaggatt tgttttgttg gagtccctta cagcgcttat
    15421 gatcgcttct gtctgtgtct acttagtgag ctttggccag ctgtttttgc ttcaggcaga
    15481 aaagaaaagt ggggaggaac tgctatttat tcgaatgatt tatgaagata tcaagacgca
    15541 gcggatgtat cacaaaagac cagagcgaag ggaagaaatg atcccatacg atatccaaat
    15601 tgtcgataat aatggagtaa tcgaaaaagt gtttgtcaat atggagaaag aagcgatcag
    15661 tattgaaaaa acaagtaatt ccagcattta cgctgattga atgtctaatt ggactgatga
    15721 tattgagcag tctattgctc acattcaatc ttttgattca gcaaaccatc caagtaagtg
    15781 aatttttgca gcgtaaagat caaaaagagt ggctaatatt tttagcacaa ttagaagaag
    15841 aactcaagag cagtcacaat gtctctgtga aaaatcagca actgcactac acgatcgagg
    15901 ataaacagta tatcatcgaa agatatcagg caatgatacg taaaagaaga acttccggcg
    15961 gacatcaacc tatgttaaca agtatcactg agcttcaatt attggaaaaa gaacacacaa
    16021 tttcttttta tgtacacttt gaaaatggag aagatggata tgccatatgg acaaaaaacg
    16081 atcagtagaa aatgttataa gggctctatt ttattttctg tcttgctttt atttctttta
    16141 tgtacgtttt tattgatggc agcattagaa agctaccgtt tgtcaatgga tcttaatata
    16201 cgaatcaaaa attattatat agcaaaaata atgaagcaga tggctttatc ggaacagtct
    16261 acgcttgccg aaagttcgga aggagttttt tactacacaa ctggatctgt aacatatcag
    16321 tgggtacaac agtccctatt cttagaggtg gaagtctcac cgtttatctt tcggttcaca
    16381 gaagaagtaa aaaatgatac agatactagt acagaatgat ggaatgttcg tgaaaacgcc
    16441 ctcttttctg ttataacact gtttttaata aaaaaatgtt tgccgtttca caaataaaaa
    16501 acagtgaaaa cgattgaaat tttgtcaatt gttcgataga attaaacagg tgaaaaaatt
    16561 gttataatat atctagaaat gaggtgcacc atgttaccag aaaaaatgga acaagggttt
    16621 aatcaaaacc tggaagctat tcagctttta cagaatgcat tgggaacttc ctttttagaa
    16681 gcctatgtcg aaaatgcaga aaatttaata gatgattatc aagtacgtgt ggtggatgga
    16741 gtgccgacaa aagagacaac gcaacggatc acagctcttt atgaagagtt gaagaaactt
    16801 tctttcgagc cagaagaatg gcgcagactg tctcaattac ttttattgaa gggcagccag
    16861 acagaacatt tgcaggcgaa ccatcaactc actcctgata gtatcggttt tttatttgtt
    16921 tttcttattg aacaactata cacaaataaa aaagcaccgg ttaagattct ggatattgcg
    16981 gcaggaatgg gcaacttgtt attaacagtt cttttaaacc tatcgaatgc ggggtaccaa
    17041 acagaaggaa ttggtgtgga tattgatgat acattgttgg ctgtcgcagc ttctacaagt
    17101 gatttgacac aagcaaatgt tcaatacttt catcaagacg ggttgcagga attattaata
    17161 gatcctgtcg attttgcaat cagcgatttg ccaatcggtt attatccgaa tgacgaaaaa
    17221 gcaaaagagt ttttaacaag taccgaagaa ggccatagtt atgcacatca tttattgcta
    17281 gagcaatcaa tgaaatatgt gaagccggat ggttttggtt tgtttttgat gccgagtgga
    17341 tttttagaaa cggatcaaag tgaagaaatc aaaaaatggt ttaaagaaga aggttatctt
    17401 caaggaatga ttcaattgcc agatgaattg ttccgtaata aacaatcgca aaaaagtatt
    17461 ttgatcttac agaaaaaagg accacaagca caacaagtaa aagaagtctt gcttgtgaaa
    17521 cttgcttcat taaaagaacc agagaaggtg acggaatttt tcaacgagtt taaaaactgg
    17581 aaatcatctg ctctgtaatc attcaacgag aaggagagtc atatgtctaa aacaattgca
    17641 atcaatgctg ggagttcaag tttgaaatgg caattatatc aaatgccaga agaaaccgta
    17701 gttgcgaaag ggatcgtcga acgtatcggt ttacaagatt ctatttttac catcaagtac
    17761 ggcgataatc aaaaattcga acaaatcatg gatatcgaaa accacgaagt agcagtaaaa
    17821 atgctgttag atcagttgat tgaactgcaa attttaagct cttatgatga aatcaccgga
    17881 gtagggcacc gtgttgttca cggaggagaa acttatgggg attcagtagt gatcgatgag
    17941 aaagtaatgg accgcattgc tgaattagca gaatttgctc ctttgcataa tcctgcaaac
    18001 ttaatgggga tcaaagcgtt taaaaagatt ttacctgaca ttttgagtgt ggctgtattc
    18061 gatacatctt tccactcaac aatgccaaag caaaattatt tatatagctt accggtgtct
    18121 tattatgaag atttcaaagt aagaaaatat ggcttccatg gcacaagtca tcgttatgtg
    18181 tcggaacgtg cggcaaaatt attgaacaaa ccgatcgaag aactaaaaat catcacttgc
    18241 cacttaggaa atggtgtatc gatcacagca gtcgatggag gtaaatccat ggatacttct
    18301 atgggattca caccattagc tggggtgaca atgggtacac gttcaggtga tatcgatcca
    18361 gctgttctcc catatttaat ggaaaaatta gagattgata ttgatgaaat gatcaatgtt
    18421 ttgaacaaga aatctggtct gctaggccta acaggattgt ctagtgatat gcgtgatctt
    18481 gaaaagaact atgaaaaaga acatattcgt ttagcttatg acattttcgt ggaccgcatc
    18541 cgaaaataca tcggcggtta tgtgacaacg atgaatggtg ttgatgcaat tgtctttact
    18601 gctggtatcg gcgagaatga cggacatgta cgtagtgaaa tcatcaaagg tatgtcttgg
    18661 tttggttgtg aaatcgatcc agcgttgaat gaattgcgtg gtgaagaagt agatatctct
    18721 acaaaagaat caaaagtaag agtattagtg atcccaacag atgaggaact aatgattgcc
    18781 cgtgatgtgg agcgtctgcg cggctaagcc aaagcattcg ttaggataaa agatgaaacg
    18841 catgcaagag ggaattcttg catgtgtttt ttttgcggag aagaccgcaa aatggtaatt
    18901 taacttcaat ttcccttctt ttttacagga tcttcttagg gaattttgct tggaaaagtg
    18961 gtcaaaaggg aaaagactgg cattttattt attgattttc aggaaaatcc caatatttta
    19021 ttttcattca tctttataag ggacattaca tatgtaaaaa gaaaaaacat ttttagatca
    19081 gcctcgtggg ctgtcgactt tattcttcgc tttatgttta gctagaaaac cgatccgaaa
    19141 cattcattga tcgaataata aaaacttgat tttccacctg tactttgcct gaatcgtgaa
    19201 agcacaggta tgaaaatcaa gttttttgtt gcgaaaaaac gtcctagtca atcagttggc
    19261 cgagcggctg gcggctgatc tgctcctgat caataacgag tgcttcttgg aagtgttgct
    19321 gcaaaaagta gcggtcatgg ctgactgcta ggagcgttcc ttgatagtct tgcagcattt
    19381 cttcaatttc ttctcgagca tagctgtcga ggtgattggt tggttcatcc aaaatcagga
    19441 cgttgatttt ttctaaaaag agtttcagca acgccaaacg gattttttca ccaccggata
    19501 aatctttgat ccgtttaccc acatcgcttt gataaaagcc acttttggct aattgccgtc
    19561 gagcgacttc ttcgtttcca gtcaatgtga tggcgtaagc cagcaaacgg ctatcgggat
    19621 cggcaaactg cagctgctgc ggcaaataac cgatttgcaa gctactacct aatttgatgg
    19681 tgccaccgta aggcttttcc tcaccgagta ataatttgag taaggttgat tttcctgcac
    19741 cattgtcgcc gatgatggct aaatgatctc cacgatagac tgcaaaactg ctgtctttga
    19801 atagtacttg atcagcgtac tgctggtgca agttttgaat gatgaaaact tctttgccag
    19861 attttccacc attagacaag ctttgcagcc gatttttcgg gggaggtggc ggtttgacga
    19921 gtgtcagttt cgccaggcgg cgttccagtt ctttggcttt tttgaagaag gactcattgt
    19981 ctccttcatg tgcccactga cgaaaacgac gaatcatgac tttcaaccgc tggacttctt
    20041 tttgctgcag ttcgtaattt tttgtcagct cagccaagcg agcttctttc aagactcgaa
    20101 aacggctgta gtttccggga tactcaatca atgcaccttc ttccagttcc catgtttttg
    20161 tggtgacttg atcgagaaat tgccgatcat gagaaacgac cagaaccgat ttttttgtat
    20221 tctgtaaata gttttccaac cagcgaatac tgttaagatc taaatgattg gtcggttcat
    20281 cgagtaaaag taaatcggca tcggctgcta aaattttggc taaagcgact ttaactcgtt
    20341 cgcctccgct aagttctgtt aaccgtgctt cctgacggtg tggaatccct aagccttgca
    20401 agatcgctgc aatccggtct tctaatacgt aaccgcctag ttcttcaaag cgatgctgca
    20461 gctcaccgta acgggccaga cacttctcta agtttgtatc tggttctgtc atcaatgctt
    20521 cgtagcgact catttgctgt ttgaggtcct gtatctcagc ggcagaatgg agcaagtagt
    20581 ggttgacggt cgcttcggaa actgtaagtt cttgttcgac cgtcccgatc tttaagcctt
    20641 ttttgcgact gataacaccg ctgtcgacac cttctctgcc ggtcaagatt tgtaaaaagg
    20701 ttgatttgcc agttccgttg ataccgacta aggcaatttt ttcaccagct tcaatttgac
    20761 aggataaatc ctcaaataat ggattcccgc cgattgtttt tttaatagaa attgcttgta
    20821 tgatcatgat ctctctcctt gagaccactc aaaaaggcca tggttatcca tggcctgtgt
    20881 tatttaaact cagattatca tgagaaaaat accttgagat ggccattgat tttacgtgaa
    20941 ttcaactgtt ttggacaaac ttgtcccgtc aacagcaact gtaaaaccaa ttgcatggct
    21001 aaatgcgata tggtatcgtt ggtaattttc attacttgtt attttagtca tgcgcatcat
    21061 agtattttcc ctccttcgtg tagttgtctt ttccagtgta cctgaaagtg tcagaaagtt
    21121 caactagcgc tgacatttac ttaatattta tcgatgtgta attctgtcag agagcttttt
    21181 ttatctgact gagggtaatg tctcgtggac cacatcattg tattctgagc taaaccagcg
    21241 ttcaggcgaa atcatatggt gtttgataaa aaactttgtc tacatgactt ccttttgcca
    21301 tttgcttaat accgttgcat taaacagctc ttctctttgg aaatctcacc aacatcgcgt
    21361 tcaggagtat cgttgtggtt gcaatggcaa gcggtagcct ttcatcaatt cttgaatgat
    21421 ttaccgaatg tagcacttgc cttctcgcgg tacccgaacc cgaaataaaa ttttagtttg
    21481 gcgataaaat cggtatccgc catcgatact tcatattttt tcgtatcaga ttcatcggaa
    21541 acttccacat tgacgaacca ataagctttg gcatgcttag gtcgttttaa accacaccag
    21601 ggcgactaat cgccctggtg ttcacttttt tttattttac ttgaactcat tgactgtttt
    21661 ccctttattt taaacattat aaagaccgat gagtgcttgc cgcgtctttt gtttttatca
    21721 cggttttttg tgcaaaaaaa cctgccgata gttggcaggt cgtctacgtg ttaagctttg
    21781 gtgtggtctt tagctggcaa ttggttttct aaatcagttc ggacaacaag cacatcgcaa
    21841 gctgcgtttc taataacata ttcagaaaca gaaccaataa ataaacgttc tacagcattc
    21901 aatcctgtgg ctcctaacat aatcaaatct acttgattat cttctgggat ttctctggca
    21961 ataatttgtt ttggtgagcc atattctacg acagaggtta cattattcaa gccagctttc
    22021 ttcgcattgc tttcataatc tgccaatgtt tgctttgcca tttcagtagc ttgttctgca
    22081 agcattccat caaaggatga aacggtttgg aaagaacgtg tgtcaatgac atgtgctaat
    22141 agaagttctc cgttatttcg gatagctaca ttgaccgcct ttttaaaagc taattcagct
    22201 tcatcagaac catctacggc aaccatgatt tttttgtatt gttgtaacat aagcattcac
    22261 tcctcaagtc aatcgtattg tactctttta ttgtagaaca ctttagggaa aaagagaatt
    22321 atttactgct ttggttcagc aatttttttt gcaaatcgga tactgaaggt ggcagaaaca
    22381 ataattacga tcagtaaaaa gaccaaggca ataaagcgtt gattgaaaaa agctgttaaa
    22441 atacccatta cgcctaaaac agcatatgca gctccgtaaa attgtaggaa tccttgattt
    22501 ttatcatttt tgttgatcaa tacaaaaaag atatcctgtt tattaaataa ataatagcta
    22561 acgaataata atactaaagt ggcgataatc attaaaatcc taatcatgaa caaatcctcc
    22621 aaaataagca acaaatagaa aaagcaattt gccaagagca aattgcttta ttctttaaaa
    22681 gatggtaatc tccgttaatg ccgttgtttg tcctgtagta ttgatcccgc gaattcaagc
    22741 aggtgggtcc catggtcgta gcttcgaact accaaatgat aaggcatgtt accagcagtg
    22801 acacaagacg ggtcccaaga tatcaataaa aatgttcggt caacacacaa agaggacaat
    22861 tcgaacatca cagatttccc acctctatag tagcatatga atttaaaaag agcaatgaga
    22921 gagctcgcag aaacagaacc gttttcttgt ctagtttttt ggaggatgct ttttccactc
    22981 ttttatacgg taatactgtt gacccagagc ctgttcatat ttccctgtat cttttggctg
    23041 ataatactga gcattcttca gtttatctgg taaatattgc tgattgaccc aagcctgatc
    23101 aaaatgatga ggatactgat aacccacgcc acggttgagt gatttcgcac ctttgtaatg
    23161 gctatctctc aaatgatcag gaacatctcc agctttgccc tctcgaatat cagcaagtgc
    23221 tgcatctaat gccatgtagg cagaattgga ttttggcgac aagcaaagat cgacgacgac
    23281 gtctgctagt gggatacgtg cctcaggcaa tcccaatttt tcagcagcta atacagcgtt
    23341 cactgtacga gccgcagcag caggattacc taaacctata tcttcataac cgataaccat
    23401 cagacgccgg caaatacttg ccagatcacc tgcctcaact aaacgggcga gataatgaag
    23461 tgcagcatcc acatcacttc cacgaatcga tttttgaaaa gccgaaataa cgtcataatg
    23521 agcatctcca tttttgtcat gggtaagagc ttttctttgg acacattctt caatgatcga
    23581 cagtgtaaga tgaatccttc cttctttatc tgaaggagtc gatagtgtag ccagttccag
    23641 cccattcaat gcactccgca aatcgccgtt cgttgcacgc gaaaggtgaa gaagagcatc
    23701 ttcgtctaac tggattgctt gttgtccaag gccgcgttct ttatccctta aagcatgttc
    23761 gactgccaac tggatgtctt gttctgttaa tggtttgact tcaaagatct gtgtacggct
    23821 gcggatcgct ggattgatgg taatataagg attctcagta gtcgctccga tcaagatgat
    23881 tcggccactt tctaaatgag gaagcagaaa atcctgtttt gttttatcta atcggtgtac
    23941 ttcatccaat aagaggatca ctgtaccact catttttgct tcttccgcta caacttgtag
    24001 atcctttttt gaatctgtag cggcgtttag catgcgaaat gcataattag tagagccggc
    24061 gatcgcactt gcgatactcg tttttccagt accaggaggt ccatatagaa tcatggaaga
    24121 gagcatgcga gcatcgacca tgcggcgtat gatttttcct tccccgacta gatgctgctg
    24181 accaacgact tcatcgatcg ttcgaggtct catgcgataa gccaatggct gttgcatagg
    24241 attttcctcc ttcttgtttt tacgtccctt aattatatca tatttagcaa gatgcctaga
    24301 aagaacagcc ttctgttgtt ttgttttcat agcttatcca actaatattg tgtatgataa
    24361 agtaggtgag aaaaatgacg gataaacaaa agatagatga atttttttgg aataaaacga
    24421 cgccagaagt ggcacgggat ttattgggga tgtatttgga gcatgacaca ccagaaggaa
    24481 gattagctgg atatattgta gacgcggaag catacctagg tccagaagac gaggccgccc
    24541 atagctatgg tttaagaaga acaccgcgtg tgcgggcaat gtatgaaaaa ccaggtacga
    24601 tctaccttta cacgatgcat actcatcgga ttttgaacat cattactcag ccagaaggga
    24661 ttcctcaagg agtaatgata cgtgcaatcg aacccgccgc aatgattgat caaatgagta
    24721 aaaatcgtgg cggaaaaact ggtccagata tcagcaacgg tccgggaaaa ttagtggaag
    24781 cattagcaat tcctcaagaa ctttatggtc agtctatagc agatagttcg ttacgcttag
    24841 tttttgagaa aaaaaagaca ccaaaaaaga tcaatgcttt gcctagaatc ggtatcccaa
    24901 acaaaggtgt atggacagaa aagccgttgc gttttgttgt gtcgggaaat ccttatatct
    24961 ctttacaaag aaaaaaccaa atcgagaaag attggggttg gaggaaagaa aatgaaaaag
    25021 aaggatctat taacatattt agatgaaata attgaaaaac aagtgacaga ctatgatgta
    25081 gctttagatt gggatacaaa aaatcatacg atcgagatcg ttgttcgctt gtttgcagaa
    25141 aataaagcac aatttgagat tgatgatgca gaagggatcg tttcgcaaga accaattatc
    25201 gaatttgaag acggcgtact tctttttaat cctcagaaat ctgtttttga tgaacgcaat
    25261 tatctagcag taatccctta tgaaggaaaa aaagggttgg caaaatcagt agctgattct
    25321 ttagtggaat atctaaatga agtattagct caagggcaaa gcgatttgct tgattttttg
    25381 gatgaagacg atgacgaagc tgtattcgag cttcactggg ataatcagca actagcagaa
    25441 ttagtagaag taaaacagca aaatgaagca gatacttatt taccgtaccc aagttattaa
    25501 ggagtgacag atatgaaatg gaatgaagta aaagtagaaa ctgaaagtga agcagtcgaa
    25561 gcagttgcta atattttgat ggaggcaggg gcgagtggtg tcgcgattga agattcttta
    25621 gatgttgaac attttcagtc ggatcaatat ggtgagattt tagataaaaa tgattttaca
    25681 tcgctgaaag aaggcgcact agtcatggct tattttccgg aaacgacttt cttaccggaa
    25741 atcttgcctt ttatcaaaga tcgtatcaca caattacctg agtttggtct agctattgga
    25801 aaaaataaag taacagttag tgaagtagag gaaagcaact gggcaacagc ctggaagaaa
    25861 tactatcatc cggtgcgtat tacgcgaatg ctgacggttg cgccaagctg ggaaaactac
    25921 cagacagaag atcctctaga aaaaattatc acactagatc ctggtatggc atttggcaca
    25981 ggcactcatc ccacgacttc cttgacattg acagcactag aaacagtctt gcgtggtggt
    26041 gaaacattgc tagatgtggg aactgggtcg ggcgtgctaa gtattgcagc aaaatatctt
    26101 ggtgcaaaag atgtctatgc ctttgattta gacgaagtag ccgttcgttc ggcgaaagaa
    26161 aatatggata tgaatgaaat agccaaagat gtccatgtat cagctaacga tcttttgaca
    26221 ggaatcgaga ttgaaagtga tgtaatcgtt gcgaacattt tagcagatat catcttattg
    26281 atgatcccag atgcttggcg tttactgaag caaacaggca cgctgatcgt ttcaggaatc
    26341 attgaagaga agaagcaaat ggttctggat gccatgttag aacaagggtt tatcgtggat
    26401 caaattttcc agcaaaaaga ctggtatgca attattttga aaaaaccaga ggtggaatag
    26461 atgcagcggt attttttaaa agaatcatat gaatcaaaag actattttgt tttagaaggg
    26521 gaaaactatc accacattgt acgggttatg aggatggaac ccgaggatga agtttttttg
    26581 gcattcaatg atcaaatagc cattgttgca gaaatcacgg agattactga agaggctgtt
    26641 tatctgaaag aaattaaaaa agaagaaaat caaaaggaac tgccaagttc gattaccata
    26701 gcgagtggct atcctaaagg ggaaaagctg gattggatcg ttcagaaatc cactgaatta
    26761 ggagcgcaag cttttcttgg tttcccggca tcagcctctg tcgttaaatg ggatgcaaaa
    26821 aaacgtatga aacgacaaca acggcttgaa aagattgctc aagaggcagc ggaacaatcc
    26881 catcggcaga tcgtgccatc agtagagtta ttggaaacga aacaagattt atttgagcgg
    26941 ctagatgatt atgacgtggt cttggttgct tatgaggaat cagcaaagca aggcgaaaaa
    27001 tcaaatgcag ccaaaattct tagtgaaaca aaaccagata gtcgaatctt agctgtcttt
    27061 ggaccagaag gtggattatc ccgcgatgaa gtcgattatt ttgtagaaca caaagggata
    27121 ctttgtggat taggaccaag aatccttcgt acagaaacag cacctcttta tttattaagt
    27181 gctgctagct atcaatggga attaatgaat ccttctttca gctaacgttc ttcattaata
    27241 aaagagtatc gacagaaaga attgaaaaaa tagtcaacaa tttgtataat agaaacaatt
    27301 atttatacaa tggatacgaa aaatcgtttg aaaaagcttt taaaaagcga acatttccgt
    27361 aaaaaaacgt ataactgcac ggattttggg acaattatca aacagatatt tgtcccgatt
    27421 cccgttttac cgaaaaaaga aagggggatc gattcctgtg acaaaagaag aaattttaac
    27481 gggacccggt gtcataaagc tcgttagtca atacatgggt cccgaacatg tagcatttgt
    27541 tgagaaagca tgcgaatacg cgactgctgc acatgacgga cagtttagaa aatcaggcga
    27601 accctacatt attcatccta tccaagtcgc aggtatatta gcagatttaa aaatggatcc
    27661 ccatacagtg gctacaggct tcttacatga tgttgttgaa gatacagaaa tcactttaga
    27721 agatctgaga gaagaatttg gcgatgacgt tgctatgtta gtggacggcg taaccaaatt
    27781 agggaaaata aaatataaat cccacgaaga gcagctagca gaaaaccacc gaaagatgct
    27841 gctagcaatg gctcaagatt tacgagttat catggtcaaa ttagctgata gactacataa
    27901 catgcgtacg ttgaagcacc tgcgagaaga taagcaaaga aggatcgctc aggaaacttt
    27961 agaaatctat gcgcctcttg cacatcgtct agggatcagc cggatcaaat gggaattaga
    28021 agatacagca cttcgttatc taaatccaaa acagtattac cgtatcgtcc acttgatgca
    28081 gacgaagaga gaagaacgtg aaaaatacgt aagtggtact gttgaagata ttcgaatagc
    28141 gacagaagag ctggggattt ttgcagaaat ctatggacgg ccaaaacaca tttattcaat
    28201 ctatcgtaaa atgaaagatc agaaaaagca gttcaacgaa atttatgact tgctagctat
    28261 tcgagtgatc gtagattcaa tcaaggattg ttatgctgta ttaggagcaa tccacacaaa
    28321 atggaaacca atgcccggca gatttaagga ttatattgcc atgccaaaag ctaatatgta
    28381 ccaatctttg catactaccg tgatcggtcc ggcaggaaat ccggtagaaa ttcaaatccg
    28441 aacacaggaa atgcatgaaa tcgctgaatt cggggttgct gcacactggg cctataaaga
    28501 aggaaaaaat gaaaaagtag aaccagatgg tatgacgaaa caattaagct ggttccatga
    28561 gatactcgaa cttcaagacg aaagctatga tgcttctgaa tttatggaag gcgtaaaagg
    28621 agatatcttt agtgataaag tctacgtctt cacaccaaaa ggagacgtta ctgagttacc
    28681 aaaaggatcc ggaccattgg actttgcata cagtatccat acagatatcg gtaacaaaac
    28741 cactggtgca aaagtaaatg gcaaaatggt gcagcttgat tacaaattga aaaacggaga
    28801 tatcattgag atcatgactt ctccaaattc atttggccca agtcgcgact ggttgaaatt
    28861 agttgctact agcaaagcaa gaaataagat caaacgtttc ttcaaagccc aagatcgaga
    28921 agaaaatgtg atcaaaggcc acgaatccgt ggtcaaatgt attacagatc taggatttac
    28981 gcctaaagat attttgacga agaacaaact gcaagaagca ctcgatcgtt ttaattatca
    29041 aacagaagat gatctctatg cagctgtagg gtatggagaa gttagcccct tgacgatggc
    29101 caatcgtctg actgaaaaag aacgtaaaga acaaaaaatc gagcagcaaa agcaagaagc
    29161 agaagaaatc atgaatcagc cgaaaaaaga acctgacaaa atgaaagtac gtcatgaagg
    29221 tggcgttgtc attcaagggg tagaaaactt actaattcgt atcagtcgct gctgtaatcc
    29281 gattcctggt gatgatatcg ttggttatat cactaaaggc agagggatat ccattcatcg
    29341 tcgagattgt ccgaatgttc agcctgacaa accaaatgta gcagaacgtt tgattgaagt
    29401 cgaatgggaa gatacatcga atacacgaaa agagtatgat gcagatttgg aaatttacgg
    29461 ctataatcgt tcaggcttat tgaatgatgt acttcaaaca gtcaatgcgc taacgaaaaa
    29521 tctcaacagc gttgaagcac ggacgaataa agataaaatg gcgacgatcc atttgacggt
    29581 tggtatccag aatttatccc acctaaagag tatcgtggat aaaatcaaag cagtacctga
    29641 tgtctacagt gtacgccgga cgaatggata ggaggacaga tatgagagca gttgttcaac
    29701 gggtaagtaa agctgctgtg tcagtcgatc agcaagaaat tggaaaaatt ggctacggcc
    29761 tacttgtttt attaggcatt catgaaaccg atacgcaaaa agatgtggac tatctggtaa
    29821 aaaaaattgc acaaatgcgt atttttgaag atgaacaagg aaaaatgaat attagtgtag
    29881 aagatgtggg aggacaaatt ttaagtatct ctcaattcac actgtttgcg gatacgaaaa
    29941 aaggaaatcg accaagcttt gtggaagccg cacgaccaga aacagctatt cctttatacg
    30001 aatcattcaa tgaaggaatc agaagtcgcg gcatcacagt cgctaccgga caattcggag
    30061 cagatatgtc ggtttctctc gtcaatgatg gacctgtaac gatcattatt gattctaaaa
    30121 acaaataaag taatctggtt ttcactagta aaaactatga aacacgtcgc ttaagttaga
    30181 ctgataaact ctaacttaag tggctttttt gtctgcaaaa agcgcttctt ttgtcagcag
    30241 tcggaaaaaa agttaagctt aaattataca agttattttt tataggaggt tgctgaagat
    30301 gaaagaagta ccagtagtag atatcaaaat cacagattta aaaaaattgt atcaaaaagg
    30361 atacaacatg ttggaggagt tacgccatga agcggacgct cccgtcgtca aagcaaagat
    30421 tttcaataaa gaagctatca cgatctacgg atcatcagca gcaaaagtat tttatgatcc
    30481 aaggaatttc aaacgaaaag gtgctatgcc aaaattagta ttgaagacac tttttggtca
    30541 aggtggagta cagactttgg atggagctgc tcatcaccat cggaaaaata tttttatgga
    30601 tctaatgaca ccggaaagaa tggaagatta tcatcgtatc ttagataaga atctgacaca
    30661 agcacttgaa gcacagcatg gtcaatttga actatttgat ttaagtaaga tggttttctt
    30721 tacatctatt tgcgagtggg caggtatcaa tttatcagcg atttctaaag atgaggtcga
    30781 aaaattagca gaatatcaaa tctctatgat cagcggaacg tttacttctc cgattgatca
    30841 cataaaaggt gtcgaaaatc gcaaaaaatc agaaaaatgg gcgcaaggct tgattgaaga
    30901 agctcgacaa aatcctgtgg ctggaaaaga aaatgtggca ttatatgctt ttgccaatgc
    30961 cacagattta gatggtcagc ttttacctct ggaagtggca gctgttgaat tattgaacat
    31021 catccgtcca acggttgctc taactgtgtg ggcggcattg atgggacatg cacttttcag
    31081 tagaccagat ttgtatcagc aattaaaaaa tgacttttcg acgttacaag atccgtttat
    31141 tcaagaaatg agacgctatt atcctttctt cccaatgcta cctgcgattt cgctgaaaga
    31201 agtagaagta gatggttatc gtataccaga aggcagttgg gttattttag atttatatgg
    31261 aacagaccat gatgaacgaa cagttgaagc gccagattcc tttatgatca aacggtatgt
    31321 agggaaagca aaagatattt cctataaaga agaatatgag atgattgcac aaggcggggg
    31381 aaattttcga caaatgcatc gctgtgcagg agaatggatc actcttcata gtctacgtgt
    31441 gttttccgat cagctagtaa ataagtttga gttctctgta cctgaacaag attggacgat
    31501 tccattcaat cagtttccga cttatccgaa tagccgagcg ctactttaca agaattagat
    31561 tagcataaac ttatcgaaaa aaaggaagat caaacagtgc tgatcttcct tttttcatat
    31621 tactataaat tgataaggtc aattgatcaa aatccacgaa atgcttcatc aatggtttga
    31681 taatcttcat tggataaatg caaatctaaa gcttggacat tgtcagatac ttgctctgga
    31741 cgcttagctc caggtataac tacagacact cgtggattct tcatgtacca agctaaaaca
    31801 agttgcgcag gagtggcttg gtatctttta gcaatggggc gaagtacatc gacagcggtt
    31861 aaaatttctc tgaaacgttc tccttggaaa tctggatttc tttttcgagg atctccttca
    31921 ccaaattgtt tttcctcacc aagttcatat tttcctgtca gcaaaccgga agcaagagga
    31981 aaatagggaa caaaactgat tttgtttttt tcaagataag ggaataattc tttttcagcc
    32041 tgccgatgga tcaaactgta tttatcttct actacatcga catatccatc tgcattcgct
    32101 tctttcaatt gttctaacgt gaagtttgac acaccgactg ctcggatttt tcctgcttct
    32161 tttaattcgt gcaatgtagc aactgattca ttttttggtg tgctttcatc cggaaagtgg
    32221 atataaaaaa tgtcgatgta atcagtttgc agacgtttta gtgcatcttc caccgattgt
    32281 ttgagaaact caggagaatt atcaaatgtt cgccctttat ttggtacatg agcagctttt
    32341 gtagcgatga cgacacgtga gcgatcatat tctttttcct tcagtacctc gccaatcagt
    32401 tcttcggaac ggccgtttcc gtaagcataa gcggtatcga tcaaattgat cccgctattc
    32461 aaagctgtac gaactacttg ttttcctgtc tcatcttcca aaccgtcaaa aagattatgt
    32521 cctccaacgg catttgctcc taatccaagc tgttctgcgt agacttgtga atgtccgatt
    32581 cttatttctg ccatagctga ttcctccttt ttgtctagtc taaaacaaaa taccaaaaag
    32641 ctgaactaaa acgtttttgt gaggggaaat agtcagatat ctcttgactt tgagactggt
    32701 ttttagtagg ttataagtaa tatcgaatgt caatgaaaga gcgagtaagt ttttgaaact
    32761 ttacaagaga ggatcatcga ggctgaaaat gatctaaagt ttcattagcc taaagacact
    32821 ctggagatgg acgagtgaaa agccgtcccg cgtacgagcg ttattcgtaa gaggaaagag
    32881 aaatctttca aaattaggtg gtaccgtgta tgaatttcat tcacccttgt tcatgatgaa
    32941 caagggtgat ttttttattt acatagctaa gaaagggaga gaaaactatg agtttccaaa
    33001 gaccaaaagg aacaaatgat attttgccag gcgaatcaga aaaatggcag tttgtcgaag
    33061 aaacagcacg tttattattc agagattatc agtataatga aatgcgtaca ccgatttttg
    33121 aacactatga agtgatatcc cgaagtgtag gggatacaac ggatatcgtt tcaaaagaga
    33181 tgtatgattt ttatgacaaa ggggaacgcc atgtgacgct tcgaccagaa ggtacagcac
    33241 caatcgttcg ttcttttgtt gagcataaat tgttcggccc agagtatgcg aaaccgttca
    33301 aaacttttta tatgggacca atgtttcgct acgaacgccc tcaaaaagga cggttacgtc
    33361 aattccatca aatcggagta gaagcatttg gatcagaaaa ccctgcaact gatgtggaga
    33421 gcatggtgat ggcacttgat ttcttcaaac agttggggat cacgcaaatc cggctagtca
    33481 tcaattcatt aggtgataaa gaaacgcgtc aagcctatcg tcaagcattg atcgactatc
    33541 ttttaccttt tgaatcagag ctgagtgaag actctaaacg ccgccttcat gaaaatccat
    33601 tacgcgtatt ggacagtaaa gacaaacgag atcaaaaatt tgtggcagaa gctccatcta
    33661 tcttagatta tctaagcgag cctgctcaaa aacattttga taccgttgtt tccatgctgg
    33721 atgcattggc tgttccttat gaaatcgaca gtaatatggt tcgcggacta gattattaca
    33781 cacatacgat ttttgaaatc atgagtgacg caccaaaaat gggggcacaa gcaacgattt
    33841 gcgcaggcgg acgttatgac aatctagtgg aagaactagg tggtccttca acacctggct
    33901 tcggatttgc aatgggtatc gagcgattac tcttgactat ggaggcagag gaagtagtta
    33961 tccctgcatt taatgagttg gatgcttacg tggtcgcact cggagacgaa acaaatatcg
    34021 aagcattgaa agtggtccaa gcgattcgaa actttggttt ttcagctgac cgcgacttca
    34081 tgaaccgtaa agcaaaagcg caatttaaga cagcagataa agcgaatgcc aaattagtat
    34141 tggtgatcgg tgaagatgag cttgctagtc aaacgattaa tgtcaaagca atggcaagcc
    34201 gaaaagaaaa aagttttcca ttagaagaga tatatgaaca ttttgatgaa gtctatgatg
    34261 aaatgacttt attcgagtag gaggaaaaaa ataaatggca caaagaacga tctattgcgg
    34321 attagtttcc gaacaacaat tagaacaaac agttacatta aaaggatggg tacaaaaaag
    34381 acgagacctt gggggagtaa tctttatcga cctacgtgat cgtgaaggaa tcgttcaaat
    34441 cgtctttaat cctgaaatca acaaagaagc atgggaaatc gcggataaat gtcgcagtga
    34501 atacgtgatc gaagtgactg gggaagttag aagacgcgga gaaaatgcag taaatcctaa
    34561 aatggccact ggtgaattcg aagtgattgc aagtgaaatc gtgattttga atcaagcaaa
    34621 aaccccgcca tttcaaattg aagatgatca agcaatcagt gacgaaatac gtttgaaata
    34681 tcgttatttg gatctgcgtc gtccaaaaat gactaaaaac atcaaattaa gaaatgcgac
    34741 aacaaaagcg attcgtcatt atttagatga taatgacttt ctcgatatcg aaacacctta
    34801 cctagggaaa tctacaccag aaggcgcaag agactaccta gttccttcac gtgtccatgc
    34861 tggacatttc tatgcgttgc cgcaatctcc tcaattgttt aaacaattgc tgatgaatgc
    34921 tggattcgac cgttattatc aaatcgttcg ttgtttccgc gatgaagatt tacgtggaga
    34981 ccgtcagcct gaatttacgc aagtagatat tgaaacaacg ttcttaacag cagaagaaat
    35041 ccaaacttac acagagggat tgattgcgaa ggtaatgaaa gaagttcgcg gtatcgaagt
    35101 cacattgcca tttcctcgta tgacctatga tgaagcaatg gctcgctacg gcagcgacaa
    35161 accggataca cgttttgata tggaattgat cgatctaagc gatacagtca aagaagtaga
    35221 attcaaagtc ttccaaatgg ctcttgaaaa cggcggtgtc gtgaaagcat tgaatgctaa
    35281 aggcgctgca gatcgctact cacgaaaaga catggatcag ttaggtcaat atgtaggaca
    35341 attcggagct aaggggcttg catggctgaa agtagaagaa gacggattga aaggtccaat
    35401 tgctaaattc atgggagaag caacagaagc aatcattaaa gcaacagatg caaaaccagg
    35461 tgacttgctg atgtttggcg cagataaaag tgaaatcgtc gctgctgcat taggtgctat
    35521 ccggacacgt ttaggaaaag aattaggact aattgatgaa tcgaaattca atttcttatg
    35581 ggtaactgat tggccacaat ttgaattttc agaagaagaa ggtcgatatg tctctgctca
    35641 ccatccattc acgatgccaa aagaagaaga tatcccgtta ttggctgaag accctgcaaa
    35701 agtctatgca gaagcttatg atatcgtctt gaacggttat gaactaggcg gaggttctat
    35761 ccgtattcat actcgtgagc ttcaagaaaa agtactagaa actctaggct ttacaaaaga
    35821 acaaatggaa gaacaatttg gtttcttgtt aacagcttta gattacggat tccctccaca
    35881 tggcggtatt gcattaggat tggaccgttt tgcaatgtta ttagccggag aagataatat
    35941 tcgtgaagtc attgcctttc ctaaaaacgg aaaagcagca gatgtgatga gtgatgcacc
    36001 aagtatggtt tcgcctttac aattgttcga attgaatatt gatgtaacga atatagaaga
    36061 atagccgata aaaaggtaaa aaggatttgt cgaatacgct tcttttgaca accttgattt
    36121 aattgaaaaa cactttataa tgaagccata acatataaag cggggctttt ccatgaaaaa
    36181 aacattcgaa aattgggctt atcatgatta cacgaccaag ctttcggtat ctatcgttta
    36241 tgcgttgatc gcatcagtcg cattgaattt cttttatcag ccaggacata tttattcaag
    36301 tggtattact ggtctagctc agattttgac gactttgtca aaatcactga taggattcga
    36361 agtaccggta tcaacaactt tgtatttact gaatatacct ttgtttgtat tggcgtggtt
    36421 aaaaatcggg aagaagttta caattttcac ttttatcaca gtcactttga cctccctttg
    36481 tatccatatt ttaccggaaa cagtcctatc taccgatcca atcatctgtg cgttgtttgg
    36541 gggggctgtg atgggactag gtattgggct agcgttgaga aacggcctat catcgggcgg
    36601 tctagatatc gtgagtatca cgattcgtaa aaaaacagga aagtcgattg gttctatttc
    36661 gatctacttc aatgcattga tcgtttttgt tgccggaatt ctatttggct ggcaatatat
    36721 gttttacagt acgttgtcta ttttcgtcag tggaaaagta actgatgtcg tttatactaa
    36781 acaaaagaag atgcaggtaa tgatcgtaac gaaacatcca gaaaaagtag taaatgaaat
    36841 ccaaaactgt atgcgtagag ggatcacgat catcaatgaa gcagaaggtg cttataagca
    36901 tgataagcag acagtattga tgacgatcgt tacacgatac gaactgcctc tacttagaga
    36961 agcaatgaaa gcttctgatc cacaagcttt tgtaagtatt gcagaaaacg tccagatact
    37021 tggtcgtttc tatgaggatg gcatttagat cgagtttgac aaaaattcat gaaatactgc
    37081 tgtagacaga cgttcgtttg tctgcagttt ttttctacca tagtagaatc agcaaagaaa
    37141 aactgctttt ttatttgaaa ttttcttaaa gttgaaaaaa agcatgttta tttttttaaa
    37201 tttatgggat gatagaaaaa aatagtagag aaaggacacg attctacttg ctattttcac
    37261 ttaatacccg tatattacga atgaaaaaag tttatgaaaa gttggaatat tgaatggaaa
    37321 aacaaaaaag gttaaaaaac agccgctaca tcactgggtt tgatgggctc cgttcattag
    37381 ctgtggtggg tgttatcttc tatcaccttc tgccaactag tatgaaaggc ggctatttgg
    37441 gcgttccgat ttttttcgtc gtatctggtt atttgatcac ggatctgctg cggcaggaat
    37501 gggaacaaaa tggaaaaatc aatatttggc aattttatgt cagaagaatg aagagactct
    37561 atccggcact tgtttttatg cttgtgacag cttcggctta tatcactttg tttcagcgag
    37621 ggctgttaaa caatcttcga ggagttgtgg tcagcagctt actgtatgtc aacaactggt
    37681 ggcaaatcaa taacggttta tcttatttcg atcgatttgc taatgagtca ccgtttacgc
    37741 atatctggtc tttagcagta gaaggtcaaa actatttggt ttggccgatc ttgtttgtat
    37801 tgttgatggt atttgttcgt aaaagaaaat ggatctttta tactgtgtta ggtacatcct
    37861 tcctctccgc catcttgatg atgatcttgt atacaccagg aggcgatcca acccgtgttt
    37921 attacggtac cgacactcgt ctgttttcta tatggttggg aagtgcgctt gcgtttgttt
    37981 ggccaagtac gcgtttaaag aaaaacatcc cgaaacaagc gaaaaatatt ctgaatatcg
    38041 ctggaggaat ctcgcttttg gcactgattc tttgcttctt ctttttagat gatcattaca
    38101 atttcatcta ttacggagga atgctcctag tgagtctgtt atgcgtagtg ctagttgcag
    38161 tgacagcaca ccccggagca agtttgaatc gatggctgac aaatccagta tttacttgga
    38221 ttggcaaacg aagctatggc atttatctgt atcagtttcc agttatgatt ttttatgaag
    38281 caaaggtgac tgatattgcc gatcatgttt tgttgcatac tttagtggaa atcacattga
    38341 ttctaatcat cagtgaactt tcttatcgat atgtggagcg tccgctagca cgttttgatt
    38401 atggtcgtac attccaagtg atgaaaggat ggttcagcaa accgatcatc agtaagagca
    38461 aaccatgggt tgttccaggc acattacttg tagcaattgc actcgttggt ttcgttacgg
    38521 caccgaaaaa cgctgtaaca gccgatcaaa aacaactgca ggcaaaaatc gctgaaagta
    38581 aaaaattagc agaagaaacc agaaacaatt cagaaaacac cactagttcg gttgaccaag
    38641 cgatccttga taagtacgag ttgtccaagg cacaagggaa taaggcacaa aaattagaac
    38701 taacggcatt tggtgattca gttatgttgg atgctgcaac ggatctacaa gaactttatc
    38761 caaaagtagt ggtagatgga gatgtcggac gacaattata tgccagtgta ccgtacattg
    38821 aaaaattaaa ggaacaagat ttgttgaaag acactgttct gatcggacta ggaacgaatg
    38881 gctcattctc ggaagctcag tttgatagtg tgatggatgc aatcggtgat cgaaaagtat
    38941 actgggtcaa tgtccgagta ccgacgaagc gctggcaaaa tgacgtgaat gcgatgcttg
    39001 aacagatgac gaaaaaatac gacaatatga cgctgattga ttggtacagc tacagtaatg
    39061 accatagtga ctggttttat gatgatcaag tccatccaaa tcctgaaggg atgacacatt
    39121 acattcactt agtcagcgaa gccttgctag gaggaagttc aagtacgaac agcagtgaga
    39181 caaacgtgac agaatgatag ctacaaaaaa cttcaggcaa aaacatttgt ttttgcctga
    39241 agttttttta tatttaccgt gtttttaaca ttcatataga ttcaggaagc aaaacggttg
    39301 agtgtgtctg taaataacgt tctttttgtt ttcgggtgag ttttttgaat gcagcttctg
    39361 cttttgttgc ttcgcttctt gaagaaaata cttctgcatg gatcatctta acaggtcttc
    39421 ttttttgaag acgagtatat ttagcaccag taccattgtt atgttcctct aatcgtctag
    39481 aaagatcggt tgtatatcca ccataaaatg tctgatcttt acagaataat acgtaaaaat
    39541 aatgttcatt tgccatagag catctcacga atctctgcag tgtattctcc atccatattg
    39601 taagtgatca atggtggagc gactttgaat ccgtctaatt tgccttcttt gattccttca
    39661 actaacaaaa tatttgcctc ttttccaggt tttgggtaaa caaattggat ccttttaggt
    39721 gcaattcgat agtcttccat gacctgaatg atttctaaga accgatcagg acgatggacc
    39781 atagtaaagc gtccatttgt ttttagtagc ttacttgaaa tatcgatcac ttcttctaac
    39841 gtcgtatgaa tctcatgacg ggcgattgcc aggtgtttat tagggttcgt tttatttgtt
    39901 ggcagccctt tgaaataagg tggattacag acgactaagt cacaagaatt atgtcgaacg
    39961 acagataagc tgtctttcag atcgatggtg tggacagtca tttgttcatt taaatggtta
    40021 agcgcgatac tacgtcttgc catgtctgct agacgaggtt gaagttcgat agcgtcgata
    40081 gccgcctggg ttcgtttact taaaaaaagt ccaactgcgc catttcctgc acaaaggtca
    40141 acaatcttcc cttttttagg tacacgggaa aagtgagcaa gcagcacagc atctaaagaa
    40201 aaagaaaaca ctttggaact ttggataatc ttgatatctt ctgcgtataa ttggtcgatt
    40261 cgttcgtctt cgtgaagcat ggattccctc caaaatgaaa aaaatagtcg tctgtattat
    40321 actattttta ttaaaaagaa ccaaggtggc ttgtcaaacc tctatttttt atgcatacta
    40381 tgaccataag aagggaaagg aagagactat gttttatcga tttatgcgcg gggtagttcg
    40441 agtactatta gccttagtta atggaaatgc ccgctatgag aataaagaag ttttaccgca
    40501 agatgagaat tacatactag ttgctcccca tcgtacttgg tgggacccat tatatctggc
    40561 ggtagctgca gcacccaagg aattcagctt tatggcaaaa gaagaactgt tcaaaaatcc
    40621 agttctgcga tttatattaa ctcgatccaa tgcttttcca gttaaaagag aaaatccagg
    40681 accaagcgtc attaaaacac ctgtaaagat tttgaaatcg acaaatttga gtttgatcat
    40741 gtttcccagt gggacaagac atgctacaga acttaaaggc ggaatggcat tgatttccag
    40801 aatgtctaaa gcgaaaatcg tgccagctgt atatcaaggt ccgttgacac ttggggatct
    40861 gttcaagcgt cagagagtga cagtgcgttt cggcgaacca atcgacttat cagatatcaa
    40921 gaaaacggat aaagaaggta tggcggagat cgaacgacga acacaagcag cttttgacaa
    40981 attggacaaa gaagtgaacc ctgatttcaa atatgaagtc aaaaaaaaat aaaatgataa
    41041 gtttatgcgt gggcggtctt ttgaatctac tgtcctgttc agttcagact ttttcaatga
    41101 aaacgaggga gagacgtcaa gtctgccctc tttttgcgtt ccaatatgta aaatagaaga
    41161 caggtgtcat gtaatggatc aaagtgttat aataattaga aggacatttt ttgaggtgat
    41221 tgaatgcgtt ttttacatac agccgattgg catatcggaa aaaaactaca aggttatgac
    41281 ttgattgaag accagcgtca tgtactatct tctatactag atatcgcgaa agaagaaaaa
    41341 gtagaggcaa tcgtgattgc cggagactta tatgatagga gtgtcccagc tgttgatgcc
    41401 gtagaattat acaatgaatt gatggtcgat tggaatttaa aggaaaagat cccgattttt
    41461 gctatatcag gaaatcacga tagcagtacg agactttcag cgggtacgcc atggttcagt
    41521 tattcgaact attttttgcg aacacagcta gcagaagcat ttgaaccagt cgagtttaaa
    41581 aacgtacaat ttttctttct tccttacttt gaaccaattg cagcgagatt atattttgaa
    41641 gatgacaata tccgaacgat caagcaagcg gtagccaagg tgatagataa aatgcaggag
    41701 ctatttgatt cagaaaaaaa acaagtattg attagccatt tttttgtaat gggaagtgta
    41761 aaatctgatt cggaaacgaa aatcgaagta ggcgggttgg attcgatccc tggtgaacta
    41821 cttacaccgt ttgattatgt agcactagga catttgcata atcaagctgc tttgagacag
    41881 gataatgctc gctatagcgg ttccccattg aaattctctt tatctgagat caaccaaaaa
    41941 aaaggcgtat ggatcgttga cctgacaaca ggcctggagt tgaattttag ggagatcgaa
    42001 cccctgcatg atattgaaga aataacagcg agtttcaaag aattgctgga tcctgccttt
    42061 tatcagtccg tcaatcggga gaattacatc tatatctatc tcactgatag aacgatcatc
    42121 ccgaatatga tgaaccagct tcggcaaata tatcctcgta ttttggggat tgaacgggca
    42181 aacggtcgag cagaacaaac aaagcgcaaa cgaaaagtac tagaaatgaa agatcctcaa
    42241 aaattagcag aagcattttt tagtgaaatg acggaagagc agctaactgc aaaccaagaa
    42301 aaatggctcg gagaaacgtt gcaagaaatc atggaaatgg aatgatcaag aatgaaaccg
    42361 aaaaaattac aacttaaaaa ttttggtcct tttatcaatg agaccgtaga tttttcacgt
    42421 ttgacagaag caccgctttt tttgatcagc gggaagacag gagctggcaa aacgacgatt
    42481 tttgatggga tcaccttcgc actttttggt gaaacttccg gaagacttcg ctctggaaaa
    42541 gagatgcgtt ctttgtttgc tacaccagac gaagaaacaa gcgtgacttt ttcttttgaa
    42601 catcagcaga tgagctatga aatcgaacga aaaccagaac aagttttagc aaaacgaaaa
    42661 ggaaacggaa caaaaaaaca ggcagccaaa gtaacgttga ccatttttga tgggaaagga
    42721 aaagaaataa aacaaatcac aaaacgtaca gaagcagatc agttgatcag agaactgatg
    42781 aatctcgatg cgaaacagtt ttctcaaatc gttttactac cccaaggaga attccgtaat
    42841 tttttggttt catcaagcag tgataaagag actgttttac gtcatttatt cggtactcaa
    42901 tttttccaac agttcaatga gcgattaaaa gaaaaagcaa aacaacagca acaaaaattg
    42961 gatcatttag agcaagagct gcatcttctg caaaaaaggt ttgttttagt caaagaagag
    43021 gaaccagcag cagggtttga agccgtttta gcccaatgga caaaccatca ggcaatactc
    43081 gaaaaacaaa tcacggaaga aaaagaagct ctaacggaac ttcaggaaaa acaaaagcaa
    43141 ctagaaaatt cctactatcg ttttgaaagc ttgaaaaaaa gttatgaaga aaaaacgata
    43201 ttaataaaca agcaaaagga attagctgct caaaaagacg aaatagagga aaagaaacga
    43261 tggattgggt attatgaatt tacgcaacga ttagtcgaac caatccagcg cctacaagaa
    43321 ataaaagttg aaaaaataga actgtcacaa aaaagtagtg agcaaacaaa gaaactagcg
    43381 atgactacgc agaattatga agagtggcaa aaaaatgaag caaaccgcgt acgccgacaa
    43441 acaaaaatca aagaagctgc tcaaaaactg caaaacaaac agatgatggt gccaattgtc
    43501 gaatccttac gtagtaaaaa acaagacgca gaacaattaa caatttcatt ggataaagaa
    43561 gaattgaatg ccgagaagct gtatcaaaat ctcgctatct atcaggaaag aactcgaatt
    43621 cttcaagaaa atttactgga tcaggaaaaa ctgcaagaag atcgtcttgc ttttggacag
    43681 ttaaaatatc tagatgaaaa gcagcaagaa atagaaaaac aaaaagcaaa agacactggg
    43741 tcgctcgcac agataagaga agaactcaaa aaagcaactg aagaaataac aaaaaatagt
    43801 cagctattga aagaacagac agaacaagca gaacagctga aaagccaatg ggcaaagcag
    43861 caaattgctc gtcttcagct ttttttgcgt ccaggggagc catgtcttgt ttgtggatca
    43921 acggaacacc ccatacaaca attaaaacac gaacagccaa ataaggaaga tatcttaaaa
    43981 gtagaacaac aactggcaga agctgagtct gctgtagaaa gaactcagca aatcattgcc
    44041 aaaagtgaag cacatgaaaa taatcttcaa caacaattat cagatttaga acaaaaaatc
    44101 atggcaacga atatacaatt ggctgaacaa gaagaacact tgcaacaggc tttgaaaaag
    44161 gcatttcctg agataacaga acaaaaaaca aaaacaactc ttgtagtaat cgaacaatct
    44221 ttagatagac gaaaaagcaa gatggccaat gcaagaatag agctggaaga aataagacaa
    44281 caagcagaaa agctcgaaca acaattagct gtctacaaac agcagcagac agaaaaaaat
    44341 aatcagctcc agcaactgca aggagaaatc aaaagtttag aacaacaatt gcaagatacg
    44401 gatagtaact gtttagaaga ggagatacga cgacttgaaa aacagttaga agaagactcg
    44461 aatcaactag ctgttgagca gaaagcagga gtgcaattgt tgcaagatct tcatacgctt
    44521 cggacacagc ttgatcttca acatgaacaa atagaaaaaa tagatgaaaa aatagaaaag
    44581 gaacaagaaa gaatagctaa aaagttagaa agccagtcgt atttttcaac aattgaagaa
    44641 atctgtgcat ttgcacaaca gcaagcacaa tataaagaaa accaacgtac agttacttct
    44701 tatcatgaag agcaattagt gacagctgac cggctgcgtc aattagaaaa tatccagcta
    44761 actgagacat tcccagatac agaagagatc agccaacagt tagaagaaat aagacgtatg
    44821 atcgaaataa agcaagaaaa gatatacggt ttacaagaac aaaaaattag taatcaaaaa
    44881 atctacgatg aatgtctttc gatttatcac acaagccaga cgaaattaga tgagcttacg
    44941 caattacagc agctttcgca aacattcaac ggagaaaatc ctaagaagac tagtttggaa
    45001 cggtatgtgc ttcaggttta tcttcaagaa gtgttacaag tagcaaatga tcatctccaa
    45061 cgtttaacga aaaatcgtta tcaatttgag ctagcggata ccattggcag ttatcgaggg
    45121 aaaaccggat tagaaatcaa tatctatgat gatcaggcag gtatgtctcg aagtgcgcat
    45181 accctgtctg gcggtgagag ttttattgca gcactttctt tagcattagc tcttgccgat
    45241 gtgatccaga cacaagctgg tggtgtagca atcgatgcat tgtttattga tgaaggattt
    45301 gggtcattag atgaagaagc tttggaaatg gcaatggaag ctttagaaac gatcgaaagt
    45361 gaaggaagaa tgatcggcat cattagtcat gtaagagaat tgaaagaacg agtattacaa
    45421 caaataagaa ttgatacaga aggtagcgga caaagcaaaa tacgctatca acttggataa
    45481 aaagggagat tggtaactat gaaatggagt attccagaaa aagtcattgc acgtggacgt
    45541 caatacttgc aagaaggccg agtgttatcg gttgtccctg accaagaaga aaacgtgtgg
    45601 catgcagaag tcttgggcag tgaattgtat atggttgatt tggatggtac agcaaaagag
    45661 atagattact gccaatgtcc ctattgggag gaacataaat attgtaagca tacagttgct
    45721 gtggaacttt atttacggtc gaaaaatcta tctcgtatca tgaaaaaaga ccagccaatg
    45781 ctagaaccgg taaaagcaac aagtgaggga gaaatcattg caaaaggttt ttctcgtcta
    45841 aaaacacaaa acaatcctca agcggtgcaa cctttgatta ttgaatacca agtagaaacg
    45901 attgagacta atcagtatca cccagagctt tctattttgg cactttactt gcgtatcggt
    45961 tcattggcat caccgaaaaa aacatatatc gtaaaaaaca tctatgattt tttacaagct
    46021 tacacagaaa aagaaactta cactgttaat aaacagtacc agtttcgtct agataaacgg
    46081 gcgtttcaaa aagaagatca aaaaatactc acgcatcttg caggaagtgc tcagacgcat
    46141 caattattag gtacgaacgg cttacaagtc aaaggaaaac tagataaaag atatctgctt
    46201 ctcccagtag aacaagcccg cttcctgtta gagcagatga atcagactac acgtttgcat
    46261 ctgcagataa atgaacaaaa cgatcaagga atacaatttt ccgatcaaaa caaaccttta
    46321 tcattcaaag tggaaaaatt tggagaagat tatttgctgc aagcattgaa tgattttgat
    46381 tttttcttta tgcattatca gtggggagtt atccaaggga cattttattc tctgacgaaa
    46441 tatcaacaaa cggtttatca aacttggaaa caattgattc gccgattgga gaaaccagaa
    46501 gtagtgttca agaaaaaaga attaccgtct ttgtttaaag agattatccc tctgctgtca
    46561 gagatcggcg acgtagctat tgcccctgca gtagaagagg aagtagcaga tatccccgtc
    46621 gaatttattt tctttttccg aaaagcaaaa ggaaaaatcc aagctcgagt cgatttcgtc
    46681 tatggcgaag tcatctattc gactgacccg aagcatgagg tctcgacaga acaggctttt
    46741 caggtgttac gtgacacagt agaagagaaa cgagtacttg atctattcag aatgtatcgc
    46801 taccagaaaa atgaaactgg ttatgaacgt ccattgccaa gcggggaaga actatatgcc
    46861 tttttccgca cagaactatc tgtcttccgt cagtttggtg aagtcagact tggaagaaaa
    46921 ttgcaagagt tgtatttaga tgcgcataaa catcaaccga aaatcgaagt aaccgagacc
    46981 ggttcatggc tcgatattcg attcgatgtg acagggatag aggagcaaga aatcgatcat
    47041 gtcctgcaaa gcttgctgag aaatgatgct ttttatacat tagaaaatgg tcaggttctt
    47101 tcttttgatt cagaagaatt tcaacaaaca agccaagtgt tgcaacaatt acgggaatcg
    47161 atacgtacag aagaaggaac gatccatgta cctaaaaatc aaggattgat cattcaaaat
    47221 caattggaaa aaagcaatgc tacttttagt gaatcgttcc aaacgatggt acaagatctc
    47281 attcatccag aaagatacca ggcacagctt cccaaaggat tgaatgcaac gatgagagat
    47341 tatcaaaagc aaggttttcg atggttgaag atgttaggtc attatcagtt tggcggtatt
    47401 ttagccgatg aaatgggact tgggaaaaca ctacagacaa ttgccttttt actttcggaa
    47461 aaagaagaac ggaaaagctt ctctgctttg atcgtagcgc ctgcgagctt aatttacaac
    47521 tggcaggcag aggtgagaaa attcgcccct tctttaagta tccaagtaat caatgggaat
    47581 aaaaaagaac gtgaagaatt actggctaaa gacacagata tccgagtgac ttcttatgcc
    47641 agcttaagac aggatctagc taattatcag tcgcaaaaaa ttgattattt gattcttgat
    47701 gaggcacaaa tggtcaaaaa tagcagtacg aaaactgcac aagcattgag agaattagct
    47761 gtacctcagc ggtttgcact gagtgggaca ccaatcgaga ataatttaga agaattgtgg
    47821 tcattgtttg caacgattat gccaggattt ttcccgacta aaacaaaatt cagggaactt
    47881 gccgcagaag aaattgcgca aatgattcga ccgtttattt tacggcgaga taaacaaact
    47941 gtgttgaaag acttgccgga aaaaacagag atgaatctat attctgcact tacagaagaa
    48001 caaaaaactg tttatttagc ttacttgcgt caaatgcgtg aagaaatcac atcaatggat
    48061 tctgaagcat tcaagaaaaa ccggatcggg atacttgctg gtctgacaag actgcgacaa
    48121 atttgctgcg atcctcgtct gtttattgaa gattaccaag gcggttccgg caagctcgaa
    48181 caagtaaaag atctactggt agctgccaaa gagaacaaac gaagagtttt gctgttttct
    48241 caatttacag ggatgctgac tatcttgcaa gaagagttag ccgagctagg gatctcgaca
    48301 ttttatttaa gaggcagtac gaaaccacaa gatcggttaa gtatggtaga cgcatttaat
    48361 gctggcgaaa aagatgtttt tctgatttcc ttaaaggctg ggggaactgg attgaatctc
    48421 acgggagcag atacggtcat cttgtatgac ctttggtgga atcctgccat tgaagaacaa
    48481 gctgctggac gtgcacatcg gatcggacaa aaaaatgtgg tagaagtgtg gcgtatgatc
    48541 tctgaaggaa cgattgagga acgcatggat tcactccagc aagaaaaacg cgaactgttc
    48601 caaaaagtga tccaaggtaa tgaagaacag ttgacgaaac tgactgaaga agacatccgc
    48661 atgattttaa gtgtaggaga aatcgaggag taagagactc gaaaaagcgt tcagtaatgg
    48721 gtaagaaagc ctttctaaag aagcgattgt ttatttgatt taaaaaatag caaggaggct
    48781 gggattccat tttatcccag cttccttgct atcatttttt tatttctatt tttaaaggtt
    48841 atacaaagat tgt
//
LOCUS       DAZKNO010000003        45044 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000003 DAZKNO010000000
VERSION     DAZKNO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.003"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..578
                     /locus_tag="ACG8MY_000099"
     CDS             27..578
                     /locus_tag="ACG8MY_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0429337.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(731..1204)
                     /locus_tag="ACG8MY_000100"
     CDS             complement(731..1204)
                     /locus_tag="ACG8MY_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0429338.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            1342..2814
                     /locus_tag="ACG8MY_000101"
     CDS             1342..2814
                     /locus_tag="ACG8MY_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0429339.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2811..3521
                     /locus_tag="ACG8MY_000102"
     CDS             2811..3521
                     /locus_tag="ACG8MY_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0429340.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            3873..4157
                     /locus_tag="ACG8MY_000103"
     CDS             3873..4157
                     /locus_tag="ACG8MY_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0429341.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(4438..4857)
                     /locus_tag="ACG8MY_000104"
     CDS             complement(4438..4857)
                     /locus_tag="ACG8MY_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0429342.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(4859..5443)
                     /locus_tag="ACG8MY_000105"
     CDS             complement(4859..5443)
                     /locus_tag="ACG8MY_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0429343.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(5490..6965)
                     /locus_tag="ACG8MY_000106"
     CDS             complement(5490..6965)
                     /locus_tag="ACG8MY_000106"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0429344.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            7146..7457
                     /locus_tag="ACG8MY_000107"
     CDS             7146..7457
                     /locus_tag="ACG8MY_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429345.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(7572..9908)
                     /locus_tag="ACG8MY_000108"
     CDS             complement(7572..9908)
                     /locus_tag="ACG8MY_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0429346.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            10050..10718
                     /locus_tag="ACG8MY_000109"
     CDS             10050..10718
                     /locus_tag="ACG8MY_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0429347.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            10721..11764
                     /locus_tag="ACG8MY_000110"
     CDS             10721..11764
                     /locus_tag="ACG8MY_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0429348.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(12001..12882)
                     /locus_tag="ACG8MY_000111"
     CDS             complement(12001..12882)
                     /locus_tag="ACG8MY_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0429349.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            13041..13769
                     /locus_tag="ACG8MY_000112"
     CDS             13041..13769
                     /locus_tag="ACG8MY_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0429350.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            13925..14242
                     /locus_tag="ACG8MY_000113"
     CDS             13925..14242
                     /locus_tag="ACG8MY_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0429351.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            14235..14843
                     /locus_tag="ACG8MY_000114"
     CDS             14235..14843
                     /locus_tag="ACG8MY_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0429352.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            14898..15689
                     /locus_tag="ACG8MY_000115"
     CDS             14898..15689
                     /locus_tag="ACG8MY_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0429353.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            15827..16315
                     /locus_tag="ACG8MY_000116"
     CDS             15827..16315
                     /locus_tag="ACG8MY_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0429354.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            16366..17238
                     /locus_tag="ACG8MY_000117"
     CDS             16366..17238
                     /locus_tag="ACG8MY_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0429355.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            17414..17776
                     /locus_tag="ACG8MY_000118"
     CDS             17414..17776
                     /locus_tag="ACG8MY_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0429356.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            17785..18537
                     /locus_tag="ACG8MY_000119"
     CDS             17785..18537
                     /locus_tag="ACG8MY_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429357.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            18551..20353
                     /locus_tag="ACG8MY_000120"
     CDS             18551..20353
                     /locus_tag="ACG8MY_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429358.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            20630..21670
                     /locus_tag="ACG8MY_000121"
     CDS             20630..21670
                     /locus_tag="ACG8MY_000121"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0429359.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(21722..23866)
                     /locus_tag="ACG8MY_000122"
     CDS             complement(21722..23866)
                     /locus_tag="ACG8MY_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0429360.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24223..25596)
                     /locus_tag="ACG8MY_000123"
     CDS             complement(24223..25596)
                     /locus_tag="ACG8MY_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0429361.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(25596..25991)
                     /gene="rbsD"
                     /locus_tag="ACG8MY_000124"
     CDS             complement(25596..25991)
                     /gene="rbsD"
                     /locus_tag="ACG8MY_000124"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0429362.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(26019..26936)
                     /gene="rbsK"
                     /locus_tag="ACG8MY_000125"
     CDS             complement(26019..26936)
                     /gene="rbsK"
                     /locus_tag="ACG8MY_000125"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0429363.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(27044..28057)
                     /locus_tag="ACG8MY_000126"
     CDS             complement(27044..28057)
                     /locus_tag="ACG8MY_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0429364.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(28138..28737)
                     /locus_tag="ACG8MY_000127"
     CDS             complement(28138..28737)
                     /locus_tag="ACG8MY_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0429365.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            28923..29624
                     /locus_tag="ACG8MY_000128"
     CDS             28923..29624
                     /locus_tag="ACG8MY_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429366.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            29634..32345
                     /locus_tag="ACG8MY_000129"
     CDS             29634..32345
                     /locus_tag="ACG8MY_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0429367.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            32796..34424
                     /gene="treC"
                     /locus_tag="ACG8MY_000130"
     CDS             32796..34424
                     /gene="treC"
                     /locus_tag="ACG8MY_000130"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0429368.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(34484..35665)
                     /locus_tag="ACG8MY_000131"
     CDS             complement(34484..35665)
                     /locus_tag="ACG8MY_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0429369.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            35917..37440
                     /locus_tag="ACG8MY_000132"
     CDS             35917..37440
                     /locus_tag="ACG8MY_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0429370.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(37509..38237)
                     /locus_tag="ACG8MY_000133"
     CDS             complement(37509..38237)
                     /locus_tag="ACG8MY_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0429371.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(38250..39530)
                     /locus_tag="ACG8MY_000134"
     CDS             complement(38250..39530)
                     /locus_tag="ACG8MY_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0429372.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(39671..40273)
                     /locus_tag="ACG8MY_000135"
     CDS             complement(39671..40273)
                     /locus_tag="ACG8MY_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0429373.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            40518..40724
                     /locus_tag="ACG8MY_000136"
     CDS             40518..40724
                     /locus_tag="ACG8MY_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429374.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            41036..41755
                     /locus_tag="ACG8MY_000137"
     CDS             41036..41755
                     /locus_tag="ACG8MY_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0429375.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(41836..42519)
                     /locus_tag="ACG8MY_000138"
     CDS             complement(41836..42519)
                     /locus_tag="ACG8MY_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0429376.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            42737..44923
                     /locus_tag="ACG8MY_000139"
     CDS             42737..44923
                     /locus_tag="ACG8MY_000139"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0429377.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        14606 a         7971 c         9042 g        13425 t
ORIGIN      
        1 aaagatttgt caaggaggga aaactgatgg caagaaaaaa tacacttcga tatattttgt
       61 tagggttatt aagtaagaag aagatgacag gatatgaatt gaatcaatcg ttcaaaaatg
      121 aaataggaga attttggcaa gcaaaacata gtcagattta tcctgaactt gcaaaaatgg
      181 aagagcaagg aatcattcag catcaagtag agatcacagg tgaaaaattg gaaaagaaag
      241 tttatgaatt aacagaagaa gggaaggaac aattggcaga gtggattcat acacccacca
      301 atgaactacc ggttaatcga gacgaatttg ttctgaaatt gtatttcgtc aaagacgtga
      361 atgatccggc attagtagaa attattcagc agcagcgtga ccttcatgaa gaaaaacgac
      421 ttcatctatt agagcgaaaa aaaacgatct ttcctactga aaaagaaaaa catgaaaatt
      481 acggacacta cctgatttta gcgcatgcga tcaatcggga aacagaatat gcaaattggc
      541 taacagatgt attagaagaa agtaaccgaa aaaaataaaa aaatatccga acagtgatgg
      601 agaattcctt tctatatgaa agaaacgcag atcaccgttc ggatattctt atgttcaaac
      661 aggccttttt tcaagtattt atgtagcatc tacttattaa aaagcttggt acttaggaga
      721 ttagtggcgt ttagagttct tttaacaact gttcgtagtc agctttttca tcgacaggaa
      781 ggagcttgta agttaattcg tctgtctcgt tgtcatgaat gatgtacatc ttagcattct
      841 cttctttgaa gttaccgatt tgtaaaatcg tatagcgttc gtcgatcaat tcgatatctt
      901 tgattgcttt tgaaaaaact ttacggacat ggtgcatttt cttttggcgg atttctgcct
      961 tttctgcagc ttttgacaaa gtaaatccgt catctaaata ttttttgatc agttctactt
     1021 ttatgactgt aggtaaacga tattttctag gacaattcgc atcagcttcg atcgaacgaa
     1081 tgaatccctt ttgttcccaa taccttaatt gacgtggaga cacacctacg atatcactta
     1141 attcactgat gccaacaacc aattgatcat tttccaacaa acgtttaagg gaagccccaa
     1201 ccatttttgt cactcctttt gtcctcttgc ttcccttaat tatatccgta ttaacttatg
     1261 tgtcaagtag gttgacaaat agcgaattta ggactaaagt attctagtag tttcagaaaa
     1321 aattcagaaa gaaggagaaa tatgagtaac aaccaacccg tagatattta cggaaaacca
     1381 tataatcgga cacttttagt tgttgtctta ttaattggta cattttgtac cgttttgaat
     1441 cagaccttat tgacgacagc ttttcctaca ttgatgaaag ctttcgatat ttcagcgtct
     1501 gatgtgcaat ggttaacgac tggtttcttg ctagtcaacg ggatcatgat cccaataaca
     1561 gcatggctga tcaataaatt cagttcaaga aatttatatc tttctgccat gacgatcttt
     1621 ttgatcggaa cgattacttg ctttacagca cctaatttta gtacattact aatcggtcgt
     1681 ttgatccaag cttgcggtgt gggagtatcg atgccattat tgcaaaatat catgctatct
     1741 attttcccgc cagaaaaaag aggatcagca atggggatgt cagggatcgt tattggactt
     1801 gctcctgctt taggccctac attatctggt tatatcattg ataactacca ttggcgtgac
     1861 ttgtttggta tggtactgcc aatcgttgtt cttgtcttgg ttttagcatt cttcctaatg
     1921 aaaagtgtta ttccattgtc aaatcctagc attgatattt tatccgctat tttatcaacg
     1981 attggattcg gtagtttact ttacggattt tctagtgtag gagatgacgg ctggggaagc
     2041 ttgaaagtaa ttggtttctt agttgttggt gtgattttca tcgccttgtt ctcatggcgt
     2101 caattacatt tggaacatcc attcttggaa cttcgtgtat tcaaatcatc tacattcacg
     2161 attgcagctg ttttagctgg tgtaacaaat atggcgatgg taggggcaga aatggtcgta
     2221 ccattgtata ttcaaaatat tcgtggagaa tcagcattcc attctggctt gatgttgttg
     2281 ccgggtgcat tgatcatggg ggctatgatg cctgtgacgg gtgcgatttt cgataaatac
     2341 ggcgcgaaac gtttagccat cacagggatg ttcattttga cagcggcaac tgcaccattt
     2401 gctttcttga caaaaacgac tccagtttta tatatcgtga tcctttatgc gatccgtatg
     2461 tttgggatct ccatggtcat gatgccggta acgacttctg gtatgaatgc attgccaaat
     2521 aacttgatca gtcatgggac agcggtaaat aatacattcc gtcaaatcgc aagctcgatt
     2581 ggtacagcta ttttgatcag tgtgttgacg aatgtgacaa aagcaaatat cccaagcaaa
     2641 tctgtattgg ataatacgcc attagcttat aaagatcagg caatcaatgc cacactgtca
     2701 ggttaccatg ccgcgtttat cgtagcgatc atctttggga ttcttggctt tattattgcc
     2761 tttttattga agaaaaaaga acctgttcta atgaaagcgg gggataatgc atgattattg
     2821 gagttttgat tgtcagcttg cttgcttttg ctttaacgaa cgtgttcgct aaaaaaacat
     2881 ggcaaacctt tttatctttg attttcggac taatcttcgt cgcttcacta agtttgatcg
     2941 tcgctaattt gtccaatcac tttggaatgg aaaaagtaac ggaaacaaaa acagaaaaaa
     3001 ttgtttccag tgctgattcc caaggagcag atatgctttt gtataaagct ttagggaatg
     3061 gaaaagaaaa agtgtatctt taccgaacaa atgaaaaaca agaaaaacca aaagcaactg
     3121 gtacggataa cgaaacaaat aaagtagaaa agacagacgg cgatgctgaa aaagtgacga
     3181 aaactactta ttgggaatac aaaaacgaca tgtataaatt ctggttcaat attgctgata
     3241 acaatcacga atatgacagc cgagtgaata cattcaaaat cccagagaca tgggtagaat
     3301 taagtacgga ccaagcagcc aagttagctg aattagtgaa gaaacagcaa agtacgatgg
     3361 aaagtgaagc aaaagcttat gttcaagacg gtatggtaaa agcaatgact gaaaatccaa
     3421 aaatgagcaa agcagagcaa gaacaacgta cgaaagcctt agctgctgaa ttccaacaac
     3481 aagcattcgc aaaattagtg aaagaagcaa aaggcgaata atattttgaa caccatgtag
     3541 gaggttgtga aaaagctgct ctgcatcaag cagtaagaac aatcagataa aaatagcact
     3601 ccatattttt catctgattg ggcttagtgc cgtagatgca agcttttgaa caccgtttaa
     3661 ggaagaggtt gtgagcctgc cgtttaactc tgagtcacaa ggagcaattt caaaaaacag
     3721 cttctcatgt ttgttgacta ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
     3781 tattcgaaaa aaggaactgc gacaagactt ttgtcacagt tccttctttt ttttgctatg
     3841 ctatactaga tgtaaaaaat aggagaaata aaatgaggga aatttattct tttgaatcaa
     3901 aaatctatgc ttccgaagtc ggcaagggcg gggcatatat tatttttccg tatgatataa
     3961 gaaaagaatt cggtgctgga agagttaaag tccaagcaac ttttgacaat gtgccatatg
     4021 aaggcagtat cgtgaatatg ggcgtgaaaa atccagatgg ttcagtctgc tacatccttg
     4081 gactacgaaa agatattcgt aaaaacatcg gaaaagaaat cggtgatatt gtagcagtta
     4141 cagtgaagca aaaataaaag aggttgtgac aaaagacgtg tcacaacctc ttgataaacg
     4201 gtgttcaaaa gccagtttct cggtagtaag tcaaatttat gcaaaaatgt ggaaacccat
     4261 gtttacaaaa tttttcttac tactcgaaac gaagcggctt tatcacaacc tcttgataaa
     4321 cggcgttcaa tcagctgtcc ctcggtatta gctcaaaaac ggcaaaatat gagaagctat
     4381 ttttccattt ttttctctca tactcaggtc ataacgctga tttcacaacc tcttctttca
     4441 taatggtttt tcaccatctt tataataaac tggttctttg atatggaaat ctattttttc
     4501 caaccaatct aaaatcttgt aacctaactc ttctgacatc ggaaaagagg cgttgatttg
     4561 tgcattggag atatggtcaa catggatacc ggaagtgatc gtacaacttc ccggaacggc
     4621 agttttgatg atcacagcta ttttttcggc taagacatcg tctttatgga accgaccatc
     4681 gtggctaggg aaacgtacag tttgcatctc tgtatcacga gaaaatgtag tgactgttcc
     4741 gatatgtggt gtatctccgc ctgttaaagt gatcaataag tctttaccga ttactttaac
     4801 agcagcttgg atcgtgaaac ctagtttggt tacttcaact atcgtttctt tttccatatt
     4861 aatcaccctg ccaacgtttt gaatgctcat ttacaatttc aaattgatcc aaaagtttca
     4921 ttgtatggtg attaacaaga tcttggatcg tttgaggctg attataaaaa gcaggaattg
     4981 gtggtatgat ttgcacacct agtcgtgcta atttagtcaa gttttccaag tgaatggcat
     5041 ttaatggcgt ttcacgagga acaatgacta actttcgttg ctccttcatt gttacatcag
     5101 cagcacgtgc aatcaggttg tcgcctaaac caacagagat acctgcgact gttttcatac
     5161 ttgccggtac gatgatcatt ccatcggtca aaaatgatcc gctggcgaca gcagcaccta
     5221 aatctttgtt tgaataatgg taatcaaaca ggctttcgaa ttcagttagt gataagtcgg
     5281 attccagctt taagttctgt tttgcccatg gactgatgat tccgtgtgtt tctatcatcg
     5341 gatattcttt tagtttcttc actaggttga tggcataaat ggtgcctgaa gcgccactta
     5401 caccaatcac gatttttttc ttccgttgtg ccaattgttc cattttccgc actccttttt
     5461 atgatgtcaa aacagttgca gatcattcat tatttcaaat atttttccca atcttctact
     5521 tcttggaatt ttgcacgtac aaaatgttct ttcatatcga aaggaactgt accgtcaaag
     5581 atagtttttg cacttatccc acggaaacga atagagccag gattatattc tggtaattct
     5641 gatgggtcca atggatggtt cctcattcct gggatcacga tgatatcttt atctccttgg
     5701 aaacgagtgt tgaaagtcca aactacatcg ttcatgtcga aaatatcaac atcttcatcg
     5761 actaaaatga ctgtcttcaa ttctttgaag gctgataaag caagaatagc tgcttgacgc
     5821 tgaatacctt catccgcttc gttttcttta cggatttgca tgatggtcat caatttacca
     5881 ccacctgcag gaggattgta gacatttact acttttcctg gaatagcttt atcgaccaag
     5941 gcaagaatac ttgcttctgt aggaataccg gccatagata cgtgttcttc actgggacca
     6001 atcgttgttt gcatgattgg attgtctttt cggtgagtaa ctgctttgac tttcactact
     6061 tgtacagctg gattagcatc cccagtatag ccaggaaatt ctggcattgc ttttcctgag
     6121 ttcgtgttga tatcttcttg cattgtttca tttggcatga tttctgcttc aatgatgaat
     6181 tcagcacgag caatacccac ttcatcgact gctataccat caacgatttg aataggttcg
     6241 ttacgcaaag ctcctgcaca ccatagttca ttgtagccaa gtggagtggt aggtggttcg
     6301 aaagtcgtgc caatggcaat tgctggatct aaaccaatat tgatagtgat aggcattggt
     6361 ttattcaagg cttcgaattg tttttggaag tgaccaatgt ggcgtccgcc aggcatgatg
     6421 tacataccga tcgtatcttt atcttctaga accatacgat ggatcgttac atcgctcatt
     6481 gttttttctg gatctgatcc taagactaca cccattgtga tgtatggacc agcatcttca
     6541 ggagtatttg taggtgcagg caaaatgttg cgaatatcga agtctgcatc ggtagctaga
     6601 tgaacgactt cttgtgctgg agcgtctgct ttgtctactt tcacaggttt gattggattt
     6661 tctacagcgt ctttcaagaa acgaccaagt gttcgataat catggtgcaa aatttcacct
     6721 acacgtttac gactagccat tgttccgatg ttcacacgtg tgccggggaa acctttgata
     6781 ttgttgaaag tcatagttgg tccttcttga gtcggacgtt ttactgtacc gccagcaccg
     6841 atataacggt aaacaccaga taattctgct tcaggatcga tttcaatatc tgtttcgtga
     6901 tattgacctg gaagtgtttt tagttcttcg atgacttttc ttaaatcata cggttgatct
     6961 gtcatgagag attcctcctt agttgtcttt acctatctat agtaaaaggt gaaaaagagg
     7021 aaaacaattc aacttgttcg gtaaagtatt ctacaatgga atggatgcgt ttttttgata
     7081 atagaataaa ctatgatata cttatcacta aaagaaagcg catacttagt gttggaggaa
     7141 ggaaagtgaa aaaaatggag gagtcatttc ctaaagcagt aaaagtggaa aatatagcga
     7201 atattttgaa agttacattt gaaaacggag aagtaaaata cgtgaaatct cattggacag
     7261 aagagataac cgatgccttg caatttggaa aaaaaggaag aggaaaaaga aaaaatcttc
     7321 tcgcactttc tcgtaatatg tggattggga ctgaagtaac gattgaagca gatggaacag
     7381 tattcataaa tggaaaagac aggtatacgc cagaagagtt gtggtacaaa ggaaagaaaa
     7441 gtataccaga attataaaaa ataattaaaa aaaacagcgc gaaacaagga acatattact
     7501 tcctcgtttc ttgctgtttt ctactatttc agcgctctta tgactcaaat ctaaatgtgg
     7561 ttgtcacaac cttattctgc acgcaaagcg atagcggcgt cttttttcgc tgccattcgt
     7621 gcaggaagat gaccgccaag catcgtcaat atagtagaga cgatcactag aataatcgca
     7681 tggacaggat ttagttgagc aacatttttt agatccgtca tgttgtataa gatgctattg
     7741 atagggaacg tagctagcca tgcaataaac acacctaata tcccagaaga aatacctaaa
     7801 atacacgttt ctgcgtcaaa tacgcgagtg atgtcttttt tacgtgcacc taaagctttt
     7861 agtacaccga tttcttttgt ccgttcaatc acagatgtat aagtaatgat actgatcatg
     7921 atcatacttg ttactaaaga gatccctgca aaagcaataa ggacataagt gatagcatcc
     7981 atcaatccgc cagtcagttc agtcatcgtt ccagctaaat cagtataaat gattttatct
     8041 tcatctgatt tgcctttatt gtaatcatct agataatcca agatcttttc tttgtcttca
     8101 aaattgttcg gataaatcat gatacttgaa ggcaagctgt ctccacctaa ataactaagc
     8161 aatgtttgtt tggcattttc gtcaactttt tcagtagtta agacatttac accactgtct
     8221 ctttgagctt tgacgatttc tgattctttg ttttcgttga cgatttgagt cgttaattga
     8281 tcgctataag cgattcctgg agataagaga ttcattgtag aagaagattt cacgcgtagg
     8341 atccccgaga tcttcaattc atcagaagca ttctgataca tcgcatcata atctgtatta
     8401 gggataaaat tcccagttgg aagttttgta tagtaagtat tgttgtttac taatttgaat
     8461 gttgtaccaa cgatatcatc aaaatcaagt ttttcgtctt ctttcacatc aaatccaaga
     8521 ttcttcaatg cgttgatatt agtattatta ttgccgtcta cgatcagcac gacatctgtt
     8581 gctgaggctg gataacttcc ggcaagtaaa gaataattgt cttttaaaaa gttttctttg
     8641 ctagtgtcaa gctgtgtagg gaaacttgac actcccacac ctgtcattgc tgacatcgta
     8701 ctagataaag aaagagattc cgtatcagga ttttgattcg agaagctgac aggctgtact
     8761 ttgccattta catcacgtaa aagattgatt cctgtagtac gggtaaatcc gatattatta
     8821 cttaagttag gatcgatgtc tgttacgtaa tcgacgtatg tttgatcaat cttattcgtg
     8881 tgctgtgctt tatcttcttc gctgactttt gcagtaatcg ttttgctgtc tggataatcc
     8941 gctttgcttg cacccaatcc ggcatcgtca cgagtctggt tagtcgttac cttagaaata
     9001 gtaatcggaa atttcgccat cgtttcagct tgcgtgttat cgatttgttt ttggaatcca
     9061 gaagaaaggg acagaacgat cgcgattccg atgatcccaa tactggaagc aaaggaggtc
     9121 aagaaagtac gtcctttttt tgttcggata ttgttgaagg aaagctttaa cgctgtccaa
     9181 aaactcatct ttgttctgcg taagttaaat tgatcatctt ttggtcgttc gatgtgagga
     9241 tgggaatctg ttaagatctt accatcagaa aattcaatga ttcgatctgc gtattgatgg
     9301 gccagttcag gattatgtgt gaccatgatc actaattttt ctttcgataa ctcttggatc
     9361 aattccatga tctggatact tgtttctgta tctaacgctc cggttggttc atcgcacaaa
     9421 aggatatctg ggtcattggc tagtgctcga gcgatggcca ctcgctgcat ttgtccacca
     9481 gataactgat ttggcttttt atgcatatga tttgtcaagc cgactcggtg caatgcatct
     9541 tctgcccgtt ttcgtttttc gtctttagaa acaccactta atgtcatccc aagttcaacg
     9601 ttttcaataa tgcctagatg accaatcaag ttgtaacttt gaaaaatgaa accgatagag
     9661 ttgtttcggt aggcatccca gtcactgtcc ttaaagtctt tagtagactt tccgttgatt
     9721 accatatcac cggaatcata attatctagt ccaccaatca cattcagcat agtcgtttta
     9781 ccagaaccac taggacctaa aatcgctacg aattcttttt gtctgaaagc gacagacaca
     9841 ccatccaatg ctttggtggt ggtttcgccg actttataat atttttttat atcttttaat
     9901 tgaagcataa tcacccttct cccgtattga aatattcggt atactaataa gtatatcaga
     9961 acaatgatcg tagtatacga aataaatgtt aactgaagat gaactacaag aaaaaatcta
    10021 gaaatttttc aaaaaacaga gggagatcaa tgaatcgtat tttagtagta gaagatgatc
    10081 cgcaattaca gttgctttac cgttctgtgt tggaacgagc gggcttttca gtcatcaccg
    10141 cattgaatgg agaggatgcg ctgaaaaaac tagaagcgac acgtatagaa ttaatcatta
    10201 cagatatcat gatgccgaaa atggatggct atgacttgct agactcatta cgtagcacca
    10261 gatttgacac acctgttcta atgattacgg caaaggccga ttttgaggat aagaaacgcg
    10321 ggttcaaatt aggagcagat gattatatga caaaacccat cgatgtcaat gagatgatcc
    10381 tacgtgtgga agcattgctt cgacgttcaa agatcaatta cagcagtagc ttgataatcg
    10441 gacaaacaaa attagagcta gatgcttata cagtgttaca agatcagcat tcgttgattc
    10501 tgcctcaaaa agaatttctg cttctgtata aattactgtc ttatcccaac aagatcttca
    10561 ctcgccaaca gttgatggat gatatttggg gattagatac gaatacagaa gaacgaacga
    10621 tcgatgtaca tatcaaacgt ttaaggaccc gttttgaaaa gacggaagat tttcaaatca
    10681 tcacagtacg gggactaggc tataaggcgg ttatttaaag atgaaaaaaa agctattttc
    10741 acaattatgg atctattttg ctagtattgt cttcatttcg atcttagtga cactgctgtg
    10801 ttttctcggt tttattttct tattgtctcg acattctatc tcgcctgctg aatcggctgg
    10861 aaaaccaacg ttatacccgt tattcgtctt tgcggggttc agtatggtcg tgggtacagg
    10921 aatatcgatt tttgttgggc ggcgtatcct gcatccaatc agtgcgctag ggacaaatat
    10981 gagtctggta gcaacgggag atttttcgat tcgaatggat gaacagcaga aagtagcaga
    11041 agtacagcaa ctgtataaag atttcaacgt tatggtccaa gaactcaata gtatcgagac
    11101 actgaggaat gactttgtgt ccagtgtatc ccatgaattc aaaacaccac tagcaacaat
    11161 ccaaggatat gttcaattgt tacaagcccc aaatctctct gacgaagaac gccaaatatt
    11221 tttatatcgg attatcgaaa gtatcacgca gctttcacaa ttaacagaga acatcttaaa
    11281 attgaacaaa ctggaaaacc agcgtatcca actggaaaag aaagagtatc gattagatga
    11341 acaaatcaga gaagtaatcg ttttttttca gcctaaatgg gagaaagaac agctagaatt
    11401 ggatatcgaa cttgctgcag taaattacac aggcaatgaa gagtttttgt accaagtgtg
    11461 gctgaatatc atggataacg ccataaagta caatcaaatc aatggacaga ttcatatcaa
    11521 actatttgag acagccacag agattgtact ggaagtgacc gattcaggtg taggaatgaa
    11581 tgaagagacc agagatcgaa tgtttgaaaa attttatcaa ggagatacaa gtcgtcaaat
    11641 ttctgggaat gggctaggac tttcactggt taaaaaaatt ctagaactgc atgatggacg
    11701 tattgattat agcagtatcg aaggagtagg aacgacagca atgatacggt taagtaaaca
    11761 ataggaaaaa gaataaaacc cctaaaaatt ctattgaagg tttttagggg tttttagtcg
    11821 tttttttgtg attagggaaa tgttcttacg gttcttttgg cataaaaatc gctgctttct
    11881 tattgaacta cgagaaatgt tagaggtgtc attgaaacaa ggacatgatg caagaaagag
    11941 agcgaaagtc atgcgtcgct ctcttttttt gggtggatag atggacagaa aatgtagggc
    12001 ttagttttct tcttgattta aatactccca aaatgtatct gaagccaaag aatgctcacc
    12061 gactttacga gtaaatgcaa gataactctc tgttgttgga gaaataggga gaatcacagt
    12121 attttctttt aatagattct tgactgtttt ttccataaca atagcaatcc ccatgtcatt
    12181 tgcaatcata ttgatgatca agtcgattct tgctccttta taagagattt ttggttcaaa
    12241 tccagcttcg cgacacagat ctagtacagg ttgatacaag ttcgtagtgg ggccaaggag
    12301 caaaaagttc tcctttttca gttcgcttag atccaatttc tttttactag ctaaaggatg
    12361 atttttaggt agaactgcta caaaatcgtc tgtttcagta ggcaaccact caatcgaact
    12421 tggtggggcc tggaaacttc tagcaaagtg gatcgtgtca ctatgttcat tggcgtcatt
    12481 gtaagttaat tcgacacttt ccacttcttt caactggact gtaaattcag gatggcgttt
    12541 caaaaactcg gtgattttgg aaaaaccttt ataattgatc attgtcggta tcgttaagat
    12601 atttaatgaa agattgttgt ctttcgtata ggtttcaagc acttcttgca tagcatggta
    12661 ttgttctacg acatttttta catatgggag caataagctt ccagcttcgg tcaattcgat
    12721 ttttctatgg gcgcgttcga ataatttgac gtctaattct gtttctaacg aaagaatctg
    12781 tttagaaata tttccttggg tggtgaattg tcgatcagca gttttggtga aattcattgt
    12841 ttcggctaaa tcaagaaaaa cttcgaattt atggatatcc atcatattcc tcctacttat
    12901 tccaataaag aatactttaa cacaaaaaat gaattggtgt aatcttctct gtctgttaca
    12961 atctttcttg cagcgaaagc aataattaca gaaatttttc tgattttttt cgcttcgtag
    13021 aaaaagagag gagttttttg atgccaactg gagttttgat caatgtaggt tccgttttgt
    13081 ttggcggact aattggcgga ttagtaggaa ataaattaag tgaacatttt aaagcacagc
    13141 tgacgatggt ttttggtgtg tgttcgatgg ggatgggaat ctattccatt gcgccgatga
    13201 aaaatatgcc ggctgttatt tttgctttag tgataggaac agcgattggt ttgattgttc
    13261 atttaggtaa tgggataaat aaaggggctg cgctgatgca agtacctatt tctaaaatct
    13321 tccctagtga aaagcttggt atgacccacg atgaatttat cagcacatta gtgacggtca
    13381 ttgttctatt ttgtgcaagt ggtacgggga tctatggaag tttagattct gggatgacag
    13441 gagacagtac gattttgatt tctaaatctg tccttgattt tttcacagca gctatttttg
    13501 cgtgtaattt agggtatgtc gtttctgtga tagcaattcc ccaattcatt attttttata
    13561 ttttgttctt attggcaaaa tttatttatc cattgactac accagacatg atcttagatt
    13621 ttaaagcttg cggcggattc ttgatggtcg ctaccggttt tagaatgatc aacgtgaaaa
    13681 tgttcccagt agctgacatg atacctgcaa tgatcgttat catgccactg agctggatgt
    13741 ggacaaattg gattatgcca ctgttataat aaaaatcaaa gagaagccat tttttgctga
    13801 ctaaaaagga tcaaatcttt tagtcagttt ttttgtattt gacaaactga ttttgaaact
    13861 atattatatg ttgtataata tagattgaaa taaaatatta cacttgagaa tagaaggtga
    13921 cgtaatgcac atccagattc caactgttct tcttgatggt actgtgctag cagcattaag
    13981 caaggaagat atgtatgggt atgccttaac aaaaatcgta caagaaagtc tctctgtcag
    14041 tgaatctaca ctatatccgg ttttgagaag attgaaaaaa gaaggatatc ttgagaccta
    14101 tgatgaacct tttgaaggca gaaaccgtcg atattactcg ttgacaccaa tgggaagtcg
    14161 tcatttagaa gaaattattg atgaatggat tgtttttcga gattctttag accggttatt
    14221 ggaggagagc acaaatgaat gaatatttga atgaagttgt ggatcatctt caagaaattc
    14281 ctgaagacga tcgttttgat ttgatccagt attatgagga atatttttta gattctggaa
    14341 aaacattaga tcagatcatt gatgaatatg gtacaccaaa gcagtttgcc ttaaaattaa
    14401 aaatcagtta cttttcagaa gaagaacaag cagaaaacga gttagtgaaa aatccaaaaa
    14461 gacaaattcg cctgatttgg ttaatcatta tcggtttatt tgcttctcct gtcctcattc
    14521 cactagcact ggggtttata ttggttattg ggggactatt ggttgcttta ctagcgctca
    14581 tttttagtat ctatgtttta tgtatcagta tcttaggtgt tggcctattc atgatcatct
    14641 caggaatcag tgtgtttgga caatcgattc tgagtggatt attctttatc ggtgtcggtc
    14701 tgttcgccac aggaagcgca gtatttattt cgccactgtt gctgaaagga acaaaagggc
    14761 ttttttatgt catgatgaac tttgttaaat ggatgggacg acgttttatt acaaaaagag
    14821 atctgcattc agcgggcgtt tgaaggagga cacataaatt gagattgaaa cattttttga
    14881 ttaccggtat tggtctgatg cttactggtg gcctcctagc aggtgcaact tatacattag
    14941 gcgctcaaaa gagtctgact tgggataatg gtcctcggtt gatcgatatg gttactgaga
    15001 caaaaaaaat caaggatgca aacaaaatcg tcatccatgc agaaaatcaa acagtttcgg
    15061 tcatacgcgg attggatttt gagataaaaa cgacttatga taagacagaa aagccagaaa
    15121 taaaggagaa aaatggcgtg ttgactgtga atgcagtcaa aaaatcaaca ggtgcaatca
    15181 ttagtttaga agacacaagt gcacaaatca ctattgttgt ccctgcagat catgaattat
    15241 ctgaattgac aatcgaaggg aataatagtg caattgatat caacgaagta tcaatcaaaa
    15301 aagtgaatac taagacacat gatggctggg tatcgttcaa tgaatttaca ggaggaacaa
    15361 taactgcgaa agatgaatta ggctcacttc gattagatga ggccaatgtg gataacttga
    15421 tcgtcacagg tgaaaacagc aacttccatc tagaagacag tttgatcagc aaagaaggaa
    15481 aagtcatcct aaacggtggc aatttatctt tgcatgagat ggaaaacagt ggctatacta
    15541 tccaaacgtc tggtgaatct tatattgaaa aagactacca gccaataaca aatactttgt
    15601 tagaacaagg acagaaaaag ctaaagatca ctgctaaaga cacatcgatc aatattacga
    15661 cggatcaaga ggacaatgaa acagattgat cctgtactca actggatatt ttaagaaaaa
    15721 acagcaaaga tccttccaat ctttgctgtt tttcatcgct taacgcacat aactatagta
    15781 aaataagaaa gaaaatatct aaaaagaaaa cagggtgaaa gagtatatga aggtagaaga
    15841 aggtcaaaga gaaaagttaa aaacagaaat ggaaagatta catacttata tcactcaact
    15901 aagccaaacc ttctatgatc cagacaaaga aaaagtgatg gttaattacc cgaacaattc
    15961 agaaggtcgc caattagagc aagtgtacca tgaagttttc aaacatttat tgacggtgaa
    16021 gaaagagctg gattattatt ctttaccgat tatcgataca ggaatattga agtatgatgg
    16081 gaagaaggaa cgatttatct ttaaatctgt tcgggaagat ttgcctcttt cagcagggat
    16141 ggatctagag gtcttggtag aagactattt tacagaagaa aagcactggg ttcgaacaaa
    16201 attggattat ttaccgcagg cagccggagg gacacaggca agcggttggt acatcactga
    16261 agacaaagag ttagaactag aaggtgtaat ggcgcgtatt cgaaaaaaaa attaaaaaag
    16321 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    16381 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    16441 ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt tttaagtatt
    16501 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    16561 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    16621 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    16681 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    16741 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    16801 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    16861 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    16921 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    16981 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    17041 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    17101 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    17161 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    17221 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    17281 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    17341 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    17401 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    17461 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    17521 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    17581 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    17641 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    17701 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    17761 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    17821 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    17881 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    17941 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    18001 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    18061 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    18121 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    18181 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    18241 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    18301 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    18361 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    18421 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    18481 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    18541 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    18601 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    18661 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    18721 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    18781 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    18841 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    18901 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    18961 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    19021 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    19081 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    19141 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    19201 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcgt
    19261 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    19321 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    19381 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    19441 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    19501 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    19561 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    19621 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    19681 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    19741 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    19801 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    19861 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    19921 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    19981 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    20041 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    20101 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    20161 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    20221 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatattctt
    20281 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    20341 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    20401 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    20461 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    20521 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    20581 catgtatgct ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    20641 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    20701 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    20761 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    20821 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    20881 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    20941 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    21001 ggtcattttc tatcagacta gcccagatat ggaaaacgga tttcctggaa ctttagatgt
    21061 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    21121 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    21181 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    21241 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    21301 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    21361 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    21421 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    21481 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    21541 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    21601 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    21661 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatttat
    21721 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
    21781 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
    21841 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
    21901 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
    21961 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    22021 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    22081 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    22141 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    22201 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    22261 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    22321 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    22381 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    22441 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    22501 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    22561 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    22621 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    22681 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    22741 cgaggtcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    22801 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    22861 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    22921 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    22981 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    23041 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    23101 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    23161 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    23221 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    23281 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    23341 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    23401 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    23461 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    23521 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    23581 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    23641 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    23701 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    23761 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
    23821 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    23881 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    23941 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    24001 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    24061 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    24121 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    24181 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    24241 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    24301 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    24361 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    24421 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    24481 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    24541 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    24601 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    24661 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    24721 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    24781 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    24841 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    24901 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    24961 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    25021 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    25081 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    25141 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    25201 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    25261 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    25321 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    25381 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    25441 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    25501 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    25561 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    25621 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    25681 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    25741 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    25801 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    25861 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    25921 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    25981 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    26041 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    26101 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    26161 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    26221 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    26281 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    26341 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    26401 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caagatcgtt
    26461 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    26521 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    26581 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    26641 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    26701 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    26761 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    26821 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    26881 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    26941 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    27001 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    27061 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    27121 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    27181 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    27241 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    27301 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    27361 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    27421 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    27481 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    27541 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    27601 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    27661 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    27721 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    27781 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    27841 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    27901 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    27961 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    28021 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    28081 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    28141 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    28201 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    28261 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    28321 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    28381 aagagaagta ccaaaaaaag cacttggatt gtattttgtg atagtcaaca tattatctgt
    28441 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    28501 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    28561 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    28621 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    28681 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    28741 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    28801 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    28861 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    28921 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    28981 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    29041 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    29101 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    29161 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    29221 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    29281 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    29341 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    29401 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    29461 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    29521 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    29581 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    29641 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    29701 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    29761 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    29821 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    29881 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    29941 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    30001 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    30061 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    30121 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    30181 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    30241 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    30301 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    30361 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    30421 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    30481 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    30541 ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    30601 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    30661 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    30721 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    30781 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    30841 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    30901 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    30961 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    31021 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    31081 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    31141 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    31201 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    31261 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    31321 aagattctct aagttctgtt tcagccaaac aatttggaac gatcattgac tatcaagcaa
    31381 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    31441 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    31501 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    31561 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    31621 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    31681 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    31741 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    31801 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    31861 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    31921 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    31981 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    32041 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    32101 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    32161 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    32221 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    32281 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    32341 aataaaaaca gaggttgtga aatcagcggt ttgacctgag tattagagaa acaaacggca
    32401 aaatagtttt tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
    32461 ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag caaaaagaat actcaaataa
    32521 aattagctcc tcatgtttta catttgagaa gactgattgc cgtagataaa agcttttgaa
    32581 caccatttat agaagaaaca agaatatttt gctattttaa acaagaggat agacaaatac
    32641 atgtctgatc tcttgttttt tattgggcaa cagacctctt agaatgtata aagataaatt
    32701 ttacaaacgc tttcggttca gttataataa taaatgataa cttgtatata taagttgtgg
    32761 taggtaataa atcagttgaa tggaggtaaa tatccatgac atttaaagac aaagtcattt
    32821 accaaatcta tcccaaatct tacaaagaca gtaatggtga tggaattgga gatttaaaag
    32881 gaatcaaaga aaaactcccg tatctgaatg atctaggcat cgacatgatt tggctaaacc
    32941 ctatttatcc aagccctcaa aaagacaacg gttatgatat ctctgattac acagccatcg
    33001 atcctatctt tggtacgatg gaggaattcg aacagctaat agccgaagca aagaaatacc
    33061 ggatagaaat catgctcgat atggtgctga atcacgtttc gacagaacat gaatggttca
    33121 aaaaagcact agcaggggac cagcattatc aggacttctt cattttaaga gaagagccaa
    33181 cggattgggt atcaaaattt ggtggaagtg cttggtcgcc atttggagat acggggaaat
    33241 attatttgca cttatatgat cggacacaag ctgacttgaa ctggcggaac caagcagtaa
    33301 gagaggaact attcaaagtt gttcgttttt ggatggaaaa aggggtaaaa ggattccgtt
    33361 tcgatgttat caatgttatc ggtaaagacg aacaattgaa aaataataaa gaaaatgatg
    33421 gcaaacctga atatacggac aaaccaatca cccatacgta tctaaaagaa ttgaaccaag
    33481 caacgtttgg gacggattcc actatcatca cagtcggtga aatgagctct acgacgatcg
    33541 aaaattgtat cttgtataca gaacctagta gagaagaatt atcaatggtc tttaatttcc
    33601 atcatttgaa agtcgattat gaaaatggga ataaatggtc tactcctcca tttgattttg
    33661 cagaattaaa gcgactattt catacatggg gagaaaagat gagtgagggt aacggctgga
    33721 atgctctatt ttttaataat catgatcagc cacgtgcgct caatcgattt gtagctatcg
    33781 atcagtatcg aaaacaagga gcagagatgc tagcaaccat gatccatttg aaccgtggga
    33841 cgccctatat ttatatggga gaagaaatcg gcatgatcga cccagactat tcatcgattt
    33901 ccgactatat tgacgtagaa agtttgaatg cttataagtt actccttgaa caaggattca
    33961 cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg cgataactct cgaacaccca
    34021 tgcaatggtc agacacagaa caagcaggat ttactacagg gaaaccttgg ttaaaagttg
    34081 gaggcaagct agaagagatt aatgtagaaa aagaactatc ttctgaagat tctattttgt
    34141 cctattacaa gaaattgatt cggctgagaa aaacatatcc aatcgttgca caaggagatt
    34201 atcatgctta tgaagcaaat catccggaag tttatggctt tcttcgccaa tttgaaggac
    34261 agcagctgct tgtattaaca aatttctatg ataaagatac aaggatcgcc atcccagaag
    34321 gattcacaga agccaatatt cttatcgata attattccga tactgttctt catcaaagtc
    34381 tggagctaaa accataccag gcaattgctt tgctgaaaaa ttaaaacaaa aacacaaaac
    34441 gtcgttccta cgagctattc tcgtaagatc gacgttttgt gttttattta aaataatctt
    34501 cttcagaaaa tgaagagttt tcatactctc ctttatcgcc ataaaattta ttgtagcgtt
    34561 gtttgaattt acggaaaata aaagtaacga gtaatttgac taacgtatag ataggaatac
    34621 caaaaataac gcccacaatt cccattagat cacccatgac aagtaaaacg attaagatcg
    34681 taatcggatg gatctgtaaa cgatcaccca ttactcgagg aatcaccaag tcaccgtgga
    34741 gaagttgtac ggcaaaccag acaatgacaa atttgacagc cataaaagta gagtcttgga
    34801 atgcaatgat caaacctgga aagaaggcga taaacggccc aatatatgga atgatacata
    34861 acaacccagc agaaatagct aaaacactag caaattctaa tccgatcagt aagaaacaaa
    34921 cccagtagac tgctccaagg ataaatgaag caatgatttg cccctttaga aatgaaccca
    34981 attgttgatt agcaatcttt gtcaaatttt taaaatctgt acgaaatgct ggtgggacaa
    35041 tcgctaaaac tgactgataa aatttttgag gatctctcaa aaggaaaaaa gcgacaattg
    35101 gaccagtaaa taaagtaata aaaatagctg taacagttga aaaaatgttg ctgatccgtt
    35161 gtgcgccact ttcccaataa tttccaataa atccagctat atcagacgtc acgtcgtcaa
    35221 tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt tttctccaaa aattgtcgga
    35281 gactctcttg aaaatcttga aacaaatctg gcaaactact gatcaatgtc tttgcctgtt
    35341 cttcaagaaa aggaataagc catatgcttc caagccccaa taaaaataaa ataatcacat
    35401 aaacgataat gactgacaag ttcctcgaaa ggtgcttgct caaacgtttg atgactggtt
    35461 ccaaaagata atacaagatc agtgcaaaca ataatggggg caagatagta gcgataatga
    35521 ttccgacagg cttgaatata aacgagatct gatggaaaaa ataaatgatc agcgcaagta
    35581 agataactat tcctaaaaca tagtaagagg tttttcctcc taaaaagcgg atcaaacgat
    35641 tcgttgtatc tgtttctttt ttcactcttt ttcctccttt atctataact tcattataga
    35701 caaaagcggg tataaaagga aaatgataga ggttgattgc aaaaatatcg tcaatcattc
    35761 atataaactt tctcatcaat taaaataaga aaacgcttga cagttctttt aaccatatga
    35821 taaattaaat cctgttgcta aaacgtaact attacggttt gcgaaaagcg aatcggaaca
    35881 ttcaaatgaa agaagatgag aagaggagtg tcataaatga aacgattgat aggtatattg
    35941 gccatgctgg ttttagcagg tatgttaact gcttgcgggg caagtgggaa agccgaggac
    36001 agtaaagaaa aattgagcgt gatgacgacc ttttatccca tgtatgattt tacaaaagca
    36061 atcgtcgggg atgaaggaga agtagagtta ttgattccag cagggactga ttcccacgat
    36121 tatgaacctt ctgctaaaga catggcaaag atccaagata cagatatctt tgtttataat
    36181 gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa cattacaaga aggcaatgtc
    36241 catacgataa aagcgacaga aggtatgctt ttacttccgg gaagtgaaga aggacatgat
    36301 catgaccatg aacacgggga agaaggccat acacatgagc tagacccaca tgtatggcta
    36361 gctccaagtt tagcaatcaa gcaggtagca aacatacgtg atcagctgat tgaagcttac
    36421 ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct ataccgagaa acttcaagca
    36481 ctccatcagc tttatcaaga aacctttaag caagcaaaac aaagatcttt tgtcacgcaa
    36541 catgctgcat tcaactatct agcgttagaa tatggattga atcaagtatc gattgctggt
    36601 ctttcttcta gtgaagaacc gtctgcggcg agaattgctg aactgaaaca ctttgttaaa
    36661 gaacatggaa tcaactatat ttattttgaa gaaaacgcca aagatagtat tgccagaacg
    36721 ttagcaaacg aagcaggtgt atctttagaa gtattgaatc ctttggaagg attgacgaat
    36781 gaacaaatag aaaatggaga aaactatctt tctatcatgg aagcaaacct cgaagcgttg
    36841 aaaaaaacga cagagacaga aaacccgcta gaagatactt tgacccccaa aaaagaaaag
    36901 agcgtctaca acggttattt tgaagacgag gatgtacaag atcgctctct ttcagattgg
    36961 gcaggaactt ggcaaacagt agatacgtat gtagaagatg ggacttttga tcctgttttt
    37021 gaatataaag aaaaattgaa tcaggataag acagcacaag aatataaaga atattatacg
    37081 aagggctatc aaacagatat tgcttcaatc aagatcagtg agaaagagat ggcatttacg
    37141 tttaagaatg gaacaacgaa acgttcaaat tatcgttatg tagggaaaga aatcttgacc
    37201 tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag cagaagataa ggaaagtggc
    37261 gcttttagat atattcaatt cagtgatcac acgattgcac cggctgactc cgaacatttt
    37321 catttatatg caggaaatga aagtcaagaa gtattgctga aagaaatgga gaattggcca
    37381 acattttatc cagatacaat ggatggacgt gaaattgcac aggaaatgct tttgcactga
    37441 taggcaaaaa agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc
    37501 ggttcttttt atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg
    37561 tttgccaacg tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata
    37621 acataggatt tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc
    37681 tcatagaatt gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc
    37741 gaagtagaaa aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct
    37801 attttgaaga tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg
    37861 atatgctcga cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt
    37921 tcgatgttgc tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt
    37981 tttcctaaag gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg
    38041 tcttctagtt cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca
    38101 ttgagaagtt cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc
    38161 atgattctat ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa
    38221 atataaatgg gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa
    38281 aaacgctggt tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca
    38341 tccaagataa acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt
    38401 tgtttcaact ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg
    38461 gaatgattct tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct
    38521 tcaatcgcat tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta
    38581 tccagttcga ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc
    38641 atgagtttta atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt
    38701 agattggccg gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa
    38761 gaccgtaatc cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt
    38821 tggtaatcat gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa
    38881 tcctgttgca tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt
    38941 ttccaataat aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta
    39001 ttcaaagcta agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag
    39061 tatgtaaata atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac
    39121 caagtctcta acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag
    39181 acaatgtgaa aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa
    39241 aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc
    39301 gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct
    39361 atagcaatta gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa
    39421 gaaatcagaa tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag
    39481 atacaaattg tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc
    39541 tcccattgat ttttaccaaa ttatagcaca attggcgtat tattttttta atttttcagg
    39601 gattttgaat tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt
    39661 agttaattgt ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt
    39721 ttgtaatagg aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa
    39781 agtaagaagc tgagaaaaat aacgtaatga gagaaaaaat tagaaacatg ctgatcgcct
    39841 tctttaataa tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc
    39901 catagcgaag cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga
    39961 tagtcaattg cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca
    40021 gcagacaaac gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat
    40081 aagaaagaaa ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa
    40141 cgaccgctag ttttccaaga ttattcaata aaacttctaa gacaaatttt acttggacat
    40201 acaccatatc ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa
    40261 ctttatcttt catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa
    40321 cgtcttttgt ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa
    40381 tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt
    40441 tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag
    40501 gtgatggagg gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat
    40561 ttaaatctga tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata
    40621 gtgtgatgat acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc
    40681 actggcagtt agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt
    40741 gagagcataa agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa
    40801 aaaacagctt ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata
    40861 ctgtttattt gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat
    40921 aggagttact tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc
    40981 ttttatttct ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc
    41041 gataattgtg atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat
    41101 gcttgcccaa gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt
    41161 gccgctattt tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa
    41221 caaacgcttt ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc
    41281 catctacgaa gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt
    41341 agaagtaaag cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc
    41401 gccacaaaag aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc
    41461 gagaatcaaa aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc
    41521 ctactataaa gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc
    41581 caaaatagtg atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca
    41641 aatcgtacaa gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc
    41701 gatccgctac actacaaata acccgaaagc aaagggaatc tttacaacta aataatataa
    41761 aaactaagca aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt
    41821 tttgattcat tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa
    41881 tgtctgagtg agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc
    41941 aaacagatga tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact
    42001 tagatagtgg aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa
    42061 gttcgttgct acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt
    42121 gattttatca aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa
    42181 tgcgggatgc tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata
    42241 tagaacaaac aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt
    42301 tgttagaaga atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag
    42361 ccattctccg tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc
    42421 aaaagcacca tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa
    42481 aagtcctatt gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga
    42541 atatttttgt cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa
    42601 actaaaatca tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa
    42661 atagacaacg aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg
    42721 gaaggaaatc aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg
    42781 ctgggagaac aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc
    42841 tacctatgaa gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa
    42901 agaaagtgaa ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt
    42961 caatcaagaa caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc
    43021 aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca
    43081 aaaacaagta gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga
    43141 actaaaaaaa cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt
    43201 ccatagtatc ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat
    43261 gcaaggaata caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca
    43321 acagctgata cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc
    43381 ttctcggtta gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt
    43441 taaagaaaaa tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta
    43501 tggattgctc acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga
    43561 tgtactccaa aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca
    43621 aaaatatgat tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat
    43681 cgtgaaacgg atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca
    43741 gagtatctat ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata
    43801 tcctaccaaa actcttttct tattaactaa ttatcgttct cgggcagcta ttgtccaagg
    43861 agcaaataga ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc
    43921 acaaaaagaa gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca
    43981 gtggctaatc aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat
    44041 catattatac cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa
    44101 tattcctttt gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt
    44161 ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa
    44221 tcaggcctac cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga
    44281 atgcgatcaa tacttttctc ctaaatatca aaatatatcc gttgcagatt atattttgtc
    44341 gataagcccc aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg
    44401 taaaacagac cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg
    44461 tatagctaag gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca
    44521 tgctactttc agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt
    44581 aaaaacccgc ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat
    44641 tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag
    44701 tttgccttat aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga
    44761 accagtaacg acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag
    44821 aaatcgtctg tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag
    44881 atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga
    44941 cagaagtggt cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt
    45001 gtgaaatttc agcttatttt cgaagaagct atttctggaa caca
//
LOCUS       DAZKNO010000004        36617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000004 DAZKNO010000000
VERSION     DAZKNO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.004"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..722)
                     /gene="pgsA"
                     /locus_tag="ACG8MY_000140"
     CDS             complement(144..722)
                     /gene="pgsA"
                     /locus_tag="ACG8MY_000140"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0429378.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(931..1854)
                     /locus_tag="ACG8MY_000141"
     CDS             complement(931..1854)
                     /locus_tag="ACG8MY_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0429379.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(2593..3879)
                     /locus_tag="ACG8MY_000142"
     CDS             complement(2593..3879)
                     /locus_tag="ACG8MY_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0429380.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(3872..5140)
                     /locus_tag="ACG8MY_000143"
     CDS             complement(3872..5140)
                     /locus_tag="ACG8MY_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0429381.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            5392..5853
                     /gene="mscL"
                     /locus_tag="ACG8MY_000144"
     CDS             5392..5853
                     /gene="mscL"
                     /locus_tag="ACG8MY_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0429382.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(5912..6358)
                     /locus_tag="ACG8MY_000145"
     CDS             complement(5912..6358)
                     /locus_tag="ACG8MY_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0429383.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(6451..6696)
                     /locus_tag="ACG8MY_000146"
     CDS             complement(6451..6696)
                     /locus_tag="ACG8MY_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0429384.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(6811..7389)
                     /locus_tag="ACG8MY_000147"
     CDS             complement(6811..7389)
                     /locus_tag="ACG8MY_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0429385.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(7466..7996)
                     /locus_tag="ACG8MY_000148"
     CDS             complement(7466..7996)
                     /locus_tag="ACG8MY_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0429386.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(8000..8860)
                     /gene="rlmB"
                     /locus_tag="ACG8MY_000149"
     CDS             complement(8000..8860)
                     /gene="rlmB"
                     /locus_tag="ACG8MY_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0429387.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(8853..9248)
                     /locus_tag="ACG8MY_000150"
     CDS             complement(8853..9248)
                     /locus_tag="ACG8MY_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0429388.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(9245..10657)
                     /gene="cysS"
                     /locus_tag="ACG8MY_000151"
     CDS             complement(9245..10657)
                     /gene="cysS"
                     /locus_tag="ACG8MY_000151"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0429389.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(10654..11190)
                     /gene="epsC"
                     /locus_tag="ACG8MY_000152"
     CDS             complement(10654..11190)
                     /gene="epsC"
                     /locus_tag="ACG8MY_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0429390.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(11276..11515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11512..12972)
                     /gene="gltX"
                     /locus_tag="ACG8MY_000153"
     CDS             complement(11512..12972)
                     /gene="gltX"
                     /locus_tag="ACG8MY_000153"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0429391.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(13142..14299)
                     /locus_tag="ACG8MY_000154"
     CDS             complement(13142..14299)
                     /locus_tag="ACG8MY_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0429392.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(14333..15703)
                     /gene="radA"
                     /locus_tag="ACG8MY_000155"
     CDS             complement(14333..15703)
                     /gene="radA"
                     /locus_tag="ACG8MY_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0429393.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(15766..16293)
                     /locus_tag="ACG8MY_000156"
     CDS             complement(15766..16293)
                     /locus_tag="ACG8MY_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0429394.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            16523..17329
                     /gene="proB"
                     /locus_tag="ACG8MY_000157"
     CDS             16523..17329
                     /gene="proB"
                     /locus_tag="ACG8MY_000157"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0429395.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            17326..18573
                     /locus_tag="ACG8MY_000158"
     CDS             17326..18573
                     /locus_tag="ACG8MY_000158"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0429396.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            18649..18993
                     /locus_tag="ACG8MY_000159"
     CDS             18649..18993
                     /locus_tag="ACG8MY_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429397.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(19110..19436)
                     /locus_tag="ACG8MY_000160"
     CDS             complement(19110..19436)
                     /locus_tag="ACG8MY_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429398.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(19809..21010)
                     /locus_tag="ACG8MY_000161"
                     /pseudo
     CDS             complement(19809..21010)
                     /locus_tag="ACG8MY_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            21127..21642
                     /locus_tag="ACG8MY_000162"
     CDS             21127..21642
                     /locus_tag="ACG8MY_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0429399.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(21841..22287)
                     /locus_tag="ACG8MY_000163"
     CDS             complement(21841..22287)
                     /locus_tag="ACG8MY_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0429400.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(22334..23812)
                     /gene="msr(C)"
                     /locus_tag="ACG8MY_000164"
     CDS             complement(22334..23812)
                     /gene="msr(C)"
                     /locus_tag="ACG8MY_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0429401.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(24166..24795)
                     /locus_tag="ACG8MY_000165"
     CDS             complement(24166..24795)
                     /locus_tag="ACG8MY_000165"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0429402.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(24779..25243)
                     /locus_tag="ACG8MY_000166"
     CDS             complement(24779..25243)
                     /locus_tag="ACG8MY_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0429403.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(25730..26998)
                     /locus_tag="ACG8MY_000167"
     CDS             complement(25730..26998)
                     /locus_tag="ACG8MY_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0429404.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(27229..27966)
                     /locus_tag="ACG8MY_000168"
     CDS             complement(27229..27966)
                     /locus_tag="ACG8MY_000168"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0429405.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(28033..28602)
                     /locus_tag="ACG8MY_000169"
     CDS             complement(28033..28602)
                     /locus_tag="ACG8MY_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0429406.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            28730..29602
                     /locus_tag="ACG8MY_000170"
     CDS             28730..29602
                     /locus_tag="ACG8MY_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0429407.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            29944..30816
                     /locus_tag="ACG8MY_000171"
     CDS             29944..30816
                     /locus_tag="ACG8MY_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0429408.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            30813..32771
                     /gene="nagE"
                     /locus_tag="ACG8MY_000172"
     CDS             30813..32771
                     /gene="nagE"
                     /locus_tag="ACG8MY_000172"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0429409.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(33022..33873)
                     /locus_tag="ACG8MY_000173"
     CDS             complement(33022..33873)
                     /locus_tag="ACG8MY_000173"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0429410.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            33996..34463
                     /locus_tag="ACG8MY_000174"
     CDS             33996..34463
                     /locus_tag="ACG8MY_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0429411.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(34726..35208)
                     /locus_tag="ACG8MY_000175"
     CDS             complement(34726..35208)
                     /locus_tag="ACG8MY_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0429412.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(35308..36171)
                     /locus_tag="ACG8MY_000176"
     CDS             complement(35308..36171)
                     /locus_tag="ACG8MY_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0429413.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        10439 a         7759 c         6596 g        11823 t
ORIGIN      
        1 ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac aatatacata
       61 aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga cggcaggctc
      121 acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct gtttttgatg
      181 aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat gatttgatct
      241 actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat aatagcaacc
      301 atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc tccatgttcg
      361 acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat cgctactacc
      421 caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac tagcatttta
      481 tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt
      541 tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc tactagctgt
      601 gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg taaacctaca
      661 accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt tggtaaattc
      721 acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt ccaccgatat
      781 tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt gatctttttg
      841 aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt atacctacaa
      901 gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag taaggttcac
      961 attttttgga ctctgattgt tttcccctgg attaactggt acggattctc cattcgcttt
     1021 gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg tttccggtaa
     1081 cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg cattgtctac
     1141 tagcacacct aaccatacac gattgacagc agtaaattca agggttacag gcttggtagc
     1201 gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta ttgccatttt
     1261 cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg tctgcgtgct
     1321 ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag aagctgtgtc
     1381 tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga caacgacaat
     1441 cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc gacttggatg
     1501 cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct gcggacgttc
     1561 agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat ggtctccatc
     1621 ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt taccaggcaa
     1681 ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt ttgtaatctg
     1741 ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt tttctccgat
     1801 ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa gcatctgctg
     1861 ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca gctccttttt
     1921 tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata tccaataaat
     1981 ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc aggctcacga
     2041 cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa aaccggcaaa
     2101 atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg ctgatttcac
     2161 aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt
     2221 aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
     2281 ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata agccgaaata
     2341 tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact
     2401 tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag
     2461 agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa
     2521 agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca
     2581 taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt cacttataaa
     2641 tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt caggaagatc
     2701 aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat attcgatcga
     2761 atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga gatttgtttc
     2821 tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt ctgctgcttt
     2881 ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa
     2941 ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat agtttcgaga
     3001 agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc gtaacagttc
     3061 tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt tatctcgtgt
     3121 aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag ggaaataacg
     3181 tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa taagtttcat
     3241 caatttctca ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa
     3301 cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc
     3361 cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat
     3421 ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt
     3481 gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt
     3541 cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc
     3601 attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa
     3661 caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc
     3721 atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt
     3781 gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata
     3841 aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa
     3901 agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc
     3961 tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca
     4021 aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca
     4081 gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta
     4141 attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga
     4201 cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt
     4261 tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga
     4321 ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca
     4381 gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt
     4441 gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg
     4501 cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg
     4561 ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga
     4621 ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg
     4681 cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt
     4741 tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata
     4801 aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac
     4861 cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata
     4921 atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccggat
     4981 aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt
     5041 gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta
     5101 agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac
     5161 agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc
     5221 ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt
     5281 atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta
     5341 ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa
     5401 gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt
     5461 ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata
     5521 ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt
     5581 gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt
     5641 acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag
     5701 aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat
     5761 cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta
     5821 gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa agaaagccga
     5881 ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt
     5941 cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact
     6001 cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt
     6061 tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt
     6121 cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt
     6181 aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt
     6241 catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga
     6301 cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta
     6361 agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa aaagcaaacg
     6421 aaccttacga atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc
     6481 aactgtccga gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc
     6541 aggatccaaa tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc
     6601 tgtttgacgc tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc
     6661 taaatctttc ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta
     6721 taccacatat ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat
     6781 aaataagaag ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt
     6841 ttttattttg agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa
     6901 acattcagcc atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc
     6961 tatttttgaa aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat
     7021 cgctaaaggt tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt
     7081 ttggtcgatt gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa
     7141 cagagaatag atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt
     7201 gcttttttcg taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc
     7261 ctcagaaaca taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata
     7321 tttttgatac agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc
     7381 ctcagacata aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat
     7441 tcatcgaact gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt
     7501 gggacaattt ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat
     7561 gcatatcttt attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt
     7621 cataagcaga agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc
     7681 ttgtcgccac tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat
     7741 aactgtcagc cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat
     7801 tttgttggat gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat
     7861 atttcgcata atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct
     7921 gctttttaag ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca
     7981 acagctgttt tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca
     8041 aaccagcagc aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca
     8101 attcatccat ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta
     8161 aagcaattga tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa
     8221 atacccaaaa tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg
     8281 caacagggac atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa
     8341 ctgctcggtg ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa
     8401 tcgacccaaa attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt
     8461 ttttctcttt tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg
     8521 caagaacgac tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa
     8581 cccatttgac tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc
     8641 ctcgtgaatc ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg
     8701 cggcatggtg cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg
     8761 actgatttcg tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc
     8821 cacgtttcga ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga
     8881 atacaccagc ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc
     8941 atgagcgctt cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc
     9001 gtatggcttt tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga
     9061 tgttcttcca gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt
     9121 gccgcacgat gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca
     9181 tagatttcgt aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa
     9241 tctctcatgc ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt
     9301 tttctttcaa caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt
     9361 gatttcgttc ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata
     9421 aaataccgaa gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg
     9481 cagaaacttt cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg
     9541 tgatcccatt tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat
     9601 tttctaacaa agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc
     9661 ggaatttcaa attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca
     9721 tcgtcgtttc actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca
     9781 agacttgtgg atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt
     9841 tactcatttt ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag
     9901 cgaatttttt ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt
     9961 ctaaatcttg cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa
    10021 cggaacattc aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct
    10081 cgccaggttt tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt
    10141 cttcccctgt acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt
    10201 tcccataatt ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat
    10261 agcctttttc aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc
    10321 ttggatgcag tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga
    10381 tgaaacgatc agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt
    10441 tatcgtctac atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat
    10501 agcgtcgaat tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat
    10561 acacagtcgg tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt
    10621 ctttttttct ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc
    10681 tttgattcga acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt
    10741 caacacaacg gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat
    10801 ctgcgcattc gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc
    10861 ttttcctgtg ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc
    10921 ggtttcgcca atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc
    10981 tcctggatgg atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa
    11041 taaaaagagc cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa
    11101 accagggtat gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc
    11161 tgcatcaatc gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat
    11221 gtaggaaatc atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa
    11281 agacgctttc cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac
    11341 gcctgttata cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg
    11401 tttcgccaag accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc
    11461 cggtatctct cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag
    11521 tacttgattt aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa
    11581 ttctggacca tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc
    11641 ttttacgcca gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac
    11701 tacttccatt tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc
    11761 agcaagtact tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga
    11821 cagatcaacg atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt
    11881 cacccattcg attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata
    11941 aggcagacac atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac
    12001 ccattgcagt tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa
    12061 cattttgatc aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa
    12121 taatgcaata aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat
    12181 aaattgcaag attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat
    12241 caatgtcata tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg
    12301 ttttggtgta ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg
    12361 atcatcgacc gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa
    12421 gtcgccaccg atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc
    12481 gtattctgta ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt
    12541 ttctgcttgt tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc
    12601 tcttgcgcgt tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta
    12661 tgctcgattg ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga
    12721 ttgacggtaa gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa
    12781 ccaagcaagg ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt
    12841 atcctcaata cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa
    12901 tgcagttctt gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg
    12961 tactttcgtc attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc
    13021 gtcagttacg gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt
    13081 tctaaggtaa atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt
    13141 tttattttga ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg
    13201 catgtgcagg tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa
    13261 cgacttcgat acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt
    13321 catcaaggta ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca
    13381 tcgtttcacc agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa
    13441 caggtacatt ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac
    13501 catctaaaag cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt
    13561 ctccatcgta catttcgaca gatataccct cttctttttg caaggcattt aggatatcta
    13621 atccgcgacg accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt
    13681 cgtaaagaac aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat
    13741 cataaatccg accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt
    13801 ctcctgattt tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt
    13861 ttttgcttcg tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca
    13921 atcccagata gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa
    13981 tttccatatt gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac
    14041 cgatcgcacc gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt
    14101 tgataatagc tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc
    14161 ctaacaggct gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct
    14221 gccaagctga gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag
    14281 taatgacacg tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga
    14341 acacacgctt caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg
    14401 cttcccagtt acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga
    14461 cttctttgac tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt
    14521 caccgatgaa acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga
    14581 cagcgatagc taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt
    14641 aagcatcttg attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag
    14701 atacccgatt gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg
    14761 tgactaatgc ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag
    14821 cagagccagt tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt
    14881 cttccaatcc atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt
    14941 tttttactgc tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg
    15001 tatccaccat atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac
    15061 cgacgataaa aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt
    15121 ctcttacttg gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga
    15181 tggaatcaat gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca
    15241 tatctgtttc agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta
    15301 attttatctg ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg
    15361 ctaattgctg agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa
    15421 tcaagaccat agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt
    15481 ttgttttgat ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt
    15541 tttctcctgt caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga
    15601 ccatcgtatt ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc
    15661 cgcaattttg gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta
    15721 tcctaatcat gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga
    15781 accaaatccg ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa
    15841 aaagatccct tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt
    15901 ggtgaattgg aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat
    15961 cacacctact ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc
    16021 aagctgcaaa aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg
    16081 tttcaattgt ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa
    16141 cccctttttc cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa
    16201 atcatagcca gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata
    16261 tttagaaata acttcaaatc ctctttttct caccggtttt cctccctaaa taaactactc
    16321 ctattctaac atatccgagg ctgtaaaaga ctttctttac aaattcttca agaaattctg
    16381 ctatcttaaa gtaaaccatt aattcgttct ttttccaaac gactgcaagt tgtacaaaaa
    16441 aactattttt tacatgactt tactcgtcaa aacaagtaga atagaacgaa attaatgagt
    16501 ggatatttga ttgaggtgtc acatgcgtaa agaaattaaa aatgcaaaac gaattattgt
    16561 caaagtagga acaagtacat tgatttatcc caatggaaat atcaatcttg cggcaatcga
    16621 tcagttagct tttgtccttt ctgatcttag aaatcgggga aaggaagtga tcttagtctc
    16681 atcaggagct gtaggcgttg gtatgcataa attacatttg tcagaacgtc cgactttgat
    16741 tcctgaacag caagctgcgg cggctgttgg tcaagccgaa ctgatgaata tctataatca
    16801 aagattcagt gtatataatc aacagacggc acagatcctc gtgacaagag acgtggtaga
    16861 ttatcctgaa agtcgtaaaa atgtgaggaa tacattggaa cagcttttga ttatggacac
    16921 gatccctatc gtgaacgaaa acgatactgt agcagtggat gaattagatc atctgacaaa
    16981 atttggcgat aatgatcagc tgtcagccat catggcaaaa cttgtcgcgg cagacctttt
    17041 gatcgtcctt tctgatatcg acgggtttta ttcagataat ccgactacaa atcccgaggc
    17101 tcagatgtat cgctatatcg ataaaatcga tgaaaagatt ctggcacaag caggtggtgc
    17161 tggtagcaca tttggaacag gcggtatgca gagtaaatta aaagccgctt ctaggatatt
    17221 tgaagcaaac agcgcaatga ttttagcaaa tggaaaaaat ccggcaatta tttttgatat
    17281 tcttgcagga aaagaagtag gtactttatt taaggaggac ttgaaatgac tgacttgatg
    17341 caattaggac aacaggcaaa agatagcgcg caacaactag cacttatgcc tacaaaccgc
    17401 aaaaatgagc tacttgttca aatggcacaa accatcaaaa agaatcaaca agcgatcatt
    17461 gaagcaaatc ataaagactt agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa
    17521 gaccgcttgc agctgacacc tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt
    17581 gcttcgttag acgatcctat tggaaaagta gatgagatgt ggacgaacac cgacggattg
    17641 agaatcggca aaaagcgcgt gccactaggc gtgaccggca tcatttacga atctcgtcca
    17701 aatgtcacga cagatgcagc tagtctagca ttcaaatcag ggaatgccgt tatccttcgc
    17761 ggtggcaaag aagcattctt ctcgaatcaa ttattagtcc aattattgca gcaagtactg
    17821 gtcagtgctg gcgaatctcc ttatgccatc cagtttgtcg atgatacttc tcatgaaact
    17881 gcaggacagt tgatgcgatt gacagaatac ttagatgtgc tgattcctcg aggcggagca
    17941 aaattaatcc aacgagtcaa agaacaggca acggtaccta tcattgaaac aggtacagga
    18001 aataatcacg tatatatcga taaagaagca cagttatcta tggcagtgaa catcatcgtc
    18061 aatgcaaaag cttcccgtcc atctgtctgt aacgcggcag aaacattgct gatccattct
    18121 gagatcgcac cttttttctt gccagctatt gaaaaagaac tggtagaaca cggggtctct
    18181 ttacgtgcgg atgctcgtgc gttagaatat ctagagacgg ctgctttagc agaagacaca
    18241 gattgggata ctgaatatct agattatatc cttgctgtca aagtggtcga ttctttggat
    18301 gaagcaattg cacatatcaa tcggcataat actaaacatt cagaaacgat cgtgacagat
    18361 agttatgcgg ctagtcaacg tttcttaaat gaagtggacg cggcggctgt ctatgtcaat
    18421 gcctccactc gttttacaga cggatcggta tttggttttg gtgcagagat tggtatctct
    18481 actcaaaagt tgcatgcccg tggtccaatg gggttaaatg aattaacttc cacaaaatat
    18541 atcatttatg gcgatggaca aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc
    18601 ttttgccttt tttctgtaaa ggttctatcc aactagaaag gtgtcaatat gtttgaaatt
    18661 ttaatgattt tttttattta ccttatttct ctgaatatcg ctgcttttct gggagtcggt
    18721 attttatccc ttttctttca attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg
    18781 tcgcagtatt ttgacaaaat cgggcaaaaa gggctggtta cacgcttgca cattagttac
    18841 atggttgcat tatgcttatt agcgacaatc aattactatt cttttttcga tcattcaatc
    18901 gcttatacga ttacgctact gattgctggg atttttcact tatcttataa atatcagctg
    18961 aataaaaacc acttgaatcg gactttccgc taaaaaatat ccgtacgata agtagagtgc
    19021 cttgaagatc ggcaaaaaac tgccggactt tttagaggct gtgacaaaga ttttgtcaca
    19081 gcctcttttt ccatataaac ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct
    19141 tcgttaagaa gcgaaaatcc gtcaaatgtt tcgccatcat aataacgaag cagctcatcc
    19201 atggacaagt tcatcgtttt ttctttttct tcttccgctg attcagccag tagctgttcc
    19261 cgatacacct gatacatacc agctattttt tgctgcatcg cttttctagt ttcaccttcc
    19321 gcattgatta ttggcaattc tttgatatag ccacgataaa tcaaccgatc atctgaaaat
    19381 tcaatgggta attcaacgag tgtcaacccg acttcctgaa aatattcttc cagcatctat
    19441 tatcccccca ttctttttct taaatgaatt ttagcttacc tactgaaaat tttccagttt
    19501 cattattttg agaaaaaaag ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa
    19561 taaatggtgt tcttctgcca actcctcaat cataagtcac aatattcaac aaacatgaga
    19621 aactgttttt tgaaattgtt ccttgtgatt cagagttaaa cgacagtctc acaacctctt
    19681 ggtaaacggc gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg
    19741 agaagctatt tttgtttggc tgttcttatg acttgatgca ggacagcttt ttcacaacct
    19801 ctgatttctt attcttcttc atttgtagca aaataattac ggtgataaat gatacaacat
    19861 acactgataa gaacaatggc acctgcaaaa taaaacgcat agctttcacc aatatgttca
    19921 ccaatcacac ctgcaataaa tggtgctaaa gctccaccga aaaagcggac aaaactatag
    19981 ctggacgaag caacgtttcg ttgcagacct ggtatttcca tagcaatcgt cgtcaacagt
    20041 gtattgataa ttccttggaa aaatcctgct aacacaatgc tgatcggtac aaggactggc
    20101 atagaagttc cagctcctaa taacagcaaa caaataatga ataagacaaa tgctgctaaa
    20161 attgtccgat aagtcgtaaa cttccgctca agagccggtg caacgaaaat cgaagcaata
    20221 gccagaagca caccccagcc aaaaaataca aaaccgactt ctaattcaga atagccaatc
    20281 aataaaaatg gcgcatacgc aagtaaagta aagaatccga aattatataa caatgcgatt
    20341 agtccaactg aacgaagttt atgattgctt aacgctttga caccttccca aaggcctact
    20401 tttctctttg gtttctcagt ttcaggtaaa agtaccgcta gggctataaa accgcaaaac
    20461 atcaaaaatg ctaccccaaa aaatggataa cgccatgaac cactgcccaa gaccccaccg
    20521 actagagggc caacagccat tccgcagcca agtgctgctt catacatcat gattgctttt
    20581 tctgtgttac caactaaaat actgacgatc gttgctaaag cagtagagac aaataaagca
    20641 ttcccgacac cccagccggc acgtaaccca atcagcattc cgatattccc agataatccg
    20701 ccaagaccag cgaatacaac gatgataaac aatcctgtca tcaatgtttt ttcaaaccga
    20761 tccgactaga taagaaacct gtaaatagca tcaccacacc agtgactaac atgtaactag
    20821 taaataacag ggtcgtttca gctggcgtag cgtttaactg tgcagcaatc gtttttaaga
    20881 ttgggtctac tagacccact cccataaaag cgatcataca ggtagctgct acaactagcg
    20941 ctgaactttt cccctttgtt tcttcatttg tttgttcttg tatatctatt tgattctcca
    21001 atatttccat ccctttctct ctattagcat catacatgat ttattttaca tgtaaatatg
    21061 cacatgtaaa acgtggaaac tttactgtga tataataaag aaaataagac gagaggtagg
    21121 atttttatga atactttagc ctatatatta ttatgcgtgc ttttatccaa accacgttct
    21181 gggtatgaat tgaagcagct gatcacaatt ttttgggaag cccatcacag tcagatctat
    21241 acaacgcttg ccaagttaga ccagcagggt tatgtgaccg ttttggacaa agacgagcac
    21301 tctcaaaaaa agatctacca cttgactgat gaaggacgtg ctcttgtaga ggaatggatc
    21361 aaagaggaaa ctcccgctcc tacgcaaaaa gatgaatttc tagcaaaaat ctatgctttt
    21421 tcgacgctcg ataaaacaac agctactgga ctgctattca ctagagaaca acagctgaac
    21481 aaaatcatgc aaaaaaatca aaaaaaattg aatgacctct cgctagacaa gaaacaagaa
    21541 tttggacgtt atgttgtgat ccagcgcaga attatgcttt gtcagcagga actccaatgg
    21601 tgtcagcaag taagagaaca aatgcagcag ttttttgagt aagggataaa cagaaccttt
    21661 ctagaaatat ctattgaaga gcaaattatc ttcaataaaa gagcaataca gttggaaaag
    21721 aaaaagcgat ccgaacgaca ggccaaaacc tatcgttcgg atcactattt ttctagaact
    21781 gcattcaaga gactatgaca aaaagcatgc caaggccttt tttcaaataa cagtgttcca
    21841 tcattcatta ctttgaattg cttttagcac acttcttgaa agcgggccaa cgatgatcaa
    21901 ctgcaacggt aaagcggcaa tcacattcat tttccaagca ttcatataag cagagaataa
    21961 ttgatcaggt attccttgtt ccatgatcac accgaataaa gacatacatg tcaccattcc
    22021 taaaaccatc aagcatgaaa tagttaagat catttggacc ttgctttctg gatttactgg
    22081 tagtttaaag gcaatttttt tcgccagcac accgatgatg aagacatcca aaatgaaagc
    22141 caccacgaat ccaggcacta gaccaatcaa caaacttgat aaatgaagat cttgatgcag
    22201 ccataaatta tacgcgctca tccccaagac catcaaaaaa cacatcattg ttgtaaaaat
    22261 tattccttct tttttatttg taggcaatct gtaccactcc tttaaatttg tcacgaaaat
    22321 catcgtagca catttaaaaa tttctcgtaa gtactttttt tattttctgc ccttttattt
    22381 cataaacttc atcagccgta cgctcgacaa agtaagaatc atgagaagta aacaaaatcg
    22441 ttcccggata tatttgcatt agcttctcta aagcttcgat cgttgccata tcaataaaat
    22501 tagtcggttc atctaacagc aacacattac ttggcttagt aaaaagcaaa gcaatcgtca
    22561 gacgggtcgc ttctccccca cttaatgtac ataacggttt cgtcaaagtt tcctgagcaa
    22621 accctaagtg attcagcaaa ctacggacaa gcgattctga aaactctgtt tgtctcatga
    22681 caaaacgaat gatggattct tcagatgtca tttgataacc cttttgctga tatacttgaa
    22741 agcttacttt cggagagaga aggattcctt ctccttgttt gcggatatgt tctaataagg
    22801 ttgtcttacc tgaaccattt tcaccgacaa tcgcgatttt tttacctaaa gaaaactgaa
    22861 aatcaccatt taccagtaat gttctcccac ttctttccaa ttggacattt tgtcccatga
    22921 tgggatagcg gctgtggagt tcaagagatt ttggtactgg aaaacggatt tgtttcatct
    22981 gctgcggctt ggttgtttct cctatttttt ggagtcttct ctctaatgtt ttcgcattct
    23041 tttggatggc tttttgtaca ctgtcttttt gtttagagga agacaaccga tccggtctga
    23101 tttgttgctt tttcttttta ctggacactt tttccaactt ttccgcttgt tttttctttt
    23161 cttggatcga tttttccaat cgttttttct ctttcatgat cgactctgct tctcgtagct
    23221 gagtcttttt ctccaattcc ttttgagtga gataggcact ataattcccc gaaaactctc
    23281 ggatttctcc gtcttttact tcccagattt tcgatgctag ctcatctaga aaatagcgat
    23341 catgactaac aaaaaccagc gtaccataat aataacgaag ctcttcaatc agtcgatcaa
    23401 tactttttct atctagatgg gttgttggct cgtctagaag catcccttgt tcataagaag
    23461 aaagaagacg tatcaactcg atctttcgtt cttcccctcc gctccatttc cgctctgaag
    23521 tttcttccag tcttaactgg aaatgactgg ctaattccaa atttaggtcc tttgcttcag
    23581 cgagataggt taattgaggc aggtagttga aggtgatctc tgtttggatt ttcccatgat
    23641 cgggaaaaag ttcaccggct attagtttga gcaaagtcga tttaccgaca ccattttctc
    23701 ctatgatccc tatacgatct tgctgataga cacgaagaga atcaatagat agttctagct
    23761 ggttgccaaa gctgacttgt agatttgtta tatttactgc taaattttcc atatgtttta
    23821 cactcctatt tcatgtaaaa caacaattat cgatactaaa aaagagcaga aaaatctgtt
    23881 tgcaaaaaaa ctgcatcaca tcttttccta ctctgaacga aataactatg actaaatcac
    23941 caaatctgtc atagctttca cctattctac tgtcgttcga aaaaatggac atactccatc
    24001 catgaaattg ttgtttatac atgtcttttg aatttattta gttgttattc aaaagttcga
    24061 aacgtaattt catcgatgca gtcatttttc cgacctcctt ttttattagg ttaccacaca
    24121 cgattcaaaa aaagcaatag ctactgaata gtaactgaaa accttttatt cgaacgtagt
    24181 gatcttttct ggtgctaaac gaacagactc gtaaccttct tctaccgctt ttcctattga
    24241 taaaataata actggaacat aacggtcttt atccaaacca aatgcctctg ccaactgatc
    24301 tgcttcgaat ccaccaattg gatttgtttc atagccatga tcacgcgcaa ctaacatgaa
    24361 ttgcatagcc gctaagctag aatcgacttt gactacatcg ttcatttctt ctcttgaaaa
    24421 actcttgtaa taaggaatga tcgcagctaa ttgttgatct ctgacttctt ttggcatctt
    24481 ccctgattcg taagcttggt tatagatttc ttcgccatat tcgtaacatt gcatatcgcc
    24541 aaaaatcatg accattgcag aagaagtatc attttgtctc gtattgaaac ggatcaaagg
    24601 acgcaagatt tcttttgctt cctcgctttc agcgaccaca aagcgccatg gttgcatatt
    24661 gacagaagaa ggtgcagtcg tcgcttcttt gatcatttcc agcatttctt cgtgtgggat
    24721 tttgtatgtt tcatcatata cgcgaacgga tcttctacca gtcataattg ttgaaaaatc
    24781 atttttttgt tccattatta ttaacctctt tcgtttctgt tgttatattg caaagttggg
    24841 tattcaattt atctaaaagt gttagaaatt gtttcatttc tacatcgctg aaagccgggg
    24901 atagcacgga acctgtatct ttatggtcca tttcacaatg ctcgattttc ttctgggctt
    24961 tttctgttag cgctacatac acttcccggt tgttctgagg atttctctga cgcgaaacat
    25021 agccatttgc ttccaatacc ttcagatgac gagtaactgc tccttgatca atctgcaact
    25081 tttcttgcaa actgctttgt agcaatggat tttcttcttt cagtctcatc atgatctcgt
    25141 atctagtaag acttataccg atattttctt caaagatctg gacgattttt tgatcggcta
    25201 atttgatttg atataaaagg tgactaatct cttctaaaga cataatttcc tcctttctaa
    25261 taattgactc gtcaacgatt gacttgtcaa taatagaata aaaaaagaaa aaagtcaaag
    25321 aatcagctgt gtaataaaaa aaacgctctc aatagaaaag tatagagaaa agaaattttt
    25381 tgtagaatga acggaaaaaa tgataaagaa agcacaacgc taaaaccaag attattgatt
    25441 cttatcaaac tatagaagat agctcttctg cagtagaaaa ttttacaccg ccaaaaattt
    25501 ccgaaatcgc cttttgcaag aatcccgtcg aatattagaa attggatcag tatgcttaaa
    25561 cagtaataaa aaagttatga caagatgttg tcacaacctt ttttgagtaa acggtgttca
    25621 aaaacttgca tctgcggtaa taagttcaac caaacaaaaa acatgaaaat ctgtttttgt
    25681 ttggctgttc ttactacttg atgcagggca gctttttcac tacctctttt taataaccat
    25741 aaaatcctat tccttcatat ctccagccca tcggtgttag ccaatctctt tccttagcac
    25801 tcaaggtata gtgatgggta ccagtttttt catttggatt aaatagtcga taaattggct
    25861 tggaaccacc agaataaaaa cttagacctt cataacgcca accgattttt gttaaagagt
    25921 tgatctcaga ttgtgccatt gtgtaatgat ggtctccact gtaaggattg tataaacggt
    25981 agacaggttg tccgctgttt ggtgcttgcc aagcaatccc ttcatattcc cagcctacag
    26041 ttggcagata atttttttct ccggcattca atgtatataa atgttctcct gtatgacggt
    26101 tatacagacg atacatcgga gaagttgaag ttggtgctgt atcatactgt gtcagattat
    26161 aggtttcgat tacattgttc aatgtagaac cataattagg cgcagtggca taacgacctg
    26221 ttagccaagc cgtagcatct ttgtaagagt tcgtattgct tttccatgca ccggaatagt
    26281 agtaaacgcc tggctgaaaa gaagtcgtct tcaataccgt tacgttatct tgcaatgatt
    26341 ctttgtacga aggatatttt ctaaaagcgg cattcaccgt aatccattgc ccattcacat
    26401 attccgaagt aggcatcgta acagattggc cattatagct tcctttaatc ccaaatagat
    26461 tgtaattagg ggcagaagcc aaagtactgt tcccccagcc actttcaacg atggcttgag
    26521 caatcatgac tgatgcatat aagtcattgg cacttgccaa actttgtgca gttgaggcaa
    26581 tactatcaat gaacgcttgt tgagaattag cagatgagcg agcataaaaa gcatagccag
    26641 tatagttttc gaattcagac aaatctgctt gatgggtatg tgtcaatgtt tgcccatctg
    26701 tcaattggtt cgtgatttct tctgcattct ctggattgtt ctgagcttct gctgatgtac
    26761 tgtcttcggt ttgtgtttgt tctgttgttt cagttgttgg ttcttcggtt gaaattgtat
    26821 tttctgagac agtagctgac tgcaaagtac tctctgtatc gttttcaatt cctgtttctg
    26881 aagaactcga tgtctgagtt gcgacagttt ctgtcgattc ttcagctagc actgttgtgt
    26941 tggcaactgc tagatttcct aaaagaataa cgctaaataa taaattcttt gctttcatct
    27001 tttttctcct ttgaattatg aaacttttcc taaataacag tgtaactaaa aaagtaaaag
    27061 caagtcaatg tccatattta aataacattt gtttcttttt gataaatttg ctttttctac
    27121 ataaaaagac gtaaatgaag aatattcttc tccattcacg ccttaaattg atggtcgttt
    27181 gaataactat ttaacaagta attctctttg gatattatcc gttatggatt atacttcctg
    27241 tgtcgtcgga cggataatca tgttactgac agacatccgg tctggcgttt ctatcgcaaa
    27301 tgctacagca gaagctatat cttcggaggt caatccccat tctttttgcg cttcatgaag
    27361 ttcaagagca gcctttttgt cgctgatggt ttgatataat tcagtttgaa cagctcctgg
    27421 agagatgatc gtagatttga tgttattttg ccgttgctct tgacgtaatc cttccatgat
    27481 tgcacggaca gcaaattttg ttccgcaata aacggcggaa tcaggataaa caacatgtcc
    27541 cgccacagaa tcagttgaaa tgatatgccc agatttttgt ttttccatga ttggaagtac
    27601 ggcggaaatt ccatttaaca cacccatgat gttgatatca agcatatttt gccattcatc
    27661 acggtgacct tcaactaatg gagcggttgg catgattcct gcattattgt acaatacatc
    27721 gattcgaccg aacttttcca tcgctaaatc gataactgct tggacttgtg caaaatctct
    27781 tacatctgct gtttgaatat atagctcagc ttctggtaaa gattctttta ttgcttttag
    27841 gcgatcttct cggcgtgcag ctataactag tttagcccct ttttctgcta atagcttcgt
    27901 tgttgcttca ccgattccgc ttgaagcgcc catgatgaca atcacttttt ctgataaatc
    27961 attcattcta ttctcctcct gttttgaaaa atctcttgct tatactcact atctcttatt
    28021 tcatcttact gttcaaataa tccgttcgac aggtttgacg attttggcag ggattcctgc
    28081 aacgatggta ttgtctggga catctttcgt aacagttgca ttcgctgcaa caatggcatt
    28141 ctcgccaatc gttacgcctg gcagaatagt tgctcctgct ccgatccaag catttttctt
    28201 gatgtggatt ggctttactg acaagcctct tcgttctttt ggatcggtta gatggttcac
    28261 cgtgatgagg ttgacacgag gaccaatcaa tactgaatct tctaaacaga tgccacctaa
    28321 gtcaacgaac atcacttgac gattgataaa gacatttttc cctatactaa tatgtttgcc
    28381 gaaatctgta taaaaaggca gcagaacagt caccgtatca tctactttgt cactaatgat
    28441 attttctagc cgtttgcgga tatctgctgg ttcatggtac ccagtattca gcgctgctaa
    28501 aggctcttcg ttttcagctt ggatctgatg gatcacttca aagagcggat ccgttactcg
    28561 aatctcttta ttgacgattt gtttttcgat gaggcttgtc attttgctcc ctccttttat
    28621 ccttacttta taacggacgg cttatcatgt ctaatactta taaaacatac ttaactattc
    28681 cttttaacta tacttagtgg tatgcttttt taaaaacagg agggaaaaaa tggatattcg
    28741 tatactgacg tattttttag cagtagctag agaaaaaaat atcagccgag cagcaaaatc
    28801 tctgcatatc acgcaaccta ctctctcaaa acaactgaaa gatttagaag aagaacttgg
    28861 tgtgacactt ttcactcggg gctcccgaga gatacagttg acggattctg gtcgatatct
    28921 ttattcaaaa ggaaaagaaa tcttgtcgtt ggtagaaaaa acggcgaaca atttaacaaa
    28981 tgacgcaatc atctctggag aaatctacat tggcggaggc gaaactaaag caatctcttt
    29041 catttctgat agccttcatt cgttgatcaa ggcgcatcct gatatccagt ttcatttata
    29101 tagcgggaat gcagatgata tcatagacaa actggatagc ggattacttg attttggact
    29161 cgtaatcgaa ccgacaaaca aacaaaaata ccagtcattt cgactgccag cccaagatcg
    29221 ttggggactt ttgatacatc gaaatcatcc tttagcaaag caaaccgtca tccatccgca
    29281 ggaactgaaa actgttcctt tgatcatttc ccggcaaacg tcagtggaca gtcaactagc
    29341 tgaatggcta ggtttcagca tagatcagtt atcgatcgtc ggaacatata acttattgta
    29401 caatgcttct ttgatggtcg ataacgggac ttttggtgca ttagctctag atgggatcat
    29461 caatacttat gggacggaaa aaatattcat cccactcgat ccgccattac aagcttcact
    29521 gaatctcatt tggaaaaaag accagcctct ttctcctgct gctcgtaaat ttttagatgt
    29581 cctaacggag aaatacagtt aagtagagtt tttttccttc gctttcagct ttttaacaac
    29641 gaataagata agcaaaagat ccaacgatta aataacataa gtttcttaat ttatttcttc
    29701 cttttatttc tgataattcc agtaaattac tataattata aaaaaagaat tgacaaaaca
    29761 taactgtcta tataaaattg attacgttaa caataattcg tttcatgtga ctagatcata
    29821 ctaggcatga aaaatctctg aggaacatgg tttccctagg aggtttttcg tgtctatttt
    29881 tgtttggccg caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa aaagggtgcg
    29941 cgcatgaaaa tcaaaaagat ctttaatcaa aatgcagtat tggtagatga cggtggacag
    30001 gaaaaagtag ctattggaaa agggattgga tttgacagaa aaagaaatga tttgattttt
    30061 gatcgtgatg tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa gttgcaaaat
    30121 ttacttaatc agattgatga acgcttttta tttgcagcag aaacgatcat tgatcatgcg
    30181 gaaactgttt tgatggaaaa gttgaatgaa catgtattga tcgctttgac cgaccacatt
    30241 gctttttctg cggagaacat taataacggt atcgtcatcc gcaacaaact tttaagagaa
    30301 atcgaagtgc tttacagtga tgaattcagt attgctcaat gggcagtaga gtatctgaca
    30361 aaagagcttg ggattccata tacttatgac gaagcaggat atatcgcaat ccatattcac
    30421 agcggacgaa gcggtcggaa caataaccat cgcagtatcc gtgaagtgac gattgtatct
    30481 gacatcatcc atttagtgga atcagaattg gaaacagata tccattcaga accattttca
    30541 ctgaattatt caagactggt gaatcattta cgactgcttc tacaacgaac ccatgcgcaa
    30601 caatatgctg ttttagacac ggaaatcgta gaaatggtca aacgaaaata cccagaaagc
    30661 tataagattt ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct cgcgatcacg
    30721 aaagaagaac tgggttactt ggccatccat atcgagcgtt taagaagcgc aatcgtacaa
    30781 acaaatgtta acaatcatga ggaggaaaaa aattgaaagc atatctacaa cgaatgggac
    30841 gttcccttca acttccagtt gcggtcttac ctgctgctgc tttactagta gggatcggaa
    30901 actggtgggc atcttatagt aacgacattg tcgctcattt tttacaagct ggcggtaacg
    30961 cagttttagg tcaattacct ttgctgtttg ccgttgggtt agcactaggg atgtcagtgg
    31021 acaaatcagg tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca gtaaatgttt
    31081 taaaaactga ttcagtagct actttattga atatcaaacc agaatcagtt gatccatctt
    31141 ttgctcagat cagtaacgta tttattggta ttattgccgg attgattgcg gcagctcttt
    31201 ataatcgttt ccacgaagca aaattaccga tggctttatc tttctttagc ggcaagcgtc
    31261 tagtgccaat cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta ctgctcgtat
    31321 ggcctccggt ttactctgct ttagtttctt ttgctaaatt cattttgagt ttaggctggg
    31381 gtggtgcagg tctttatggc ttcttcaacc gtttactgat ccctacagga ttgcatcaag
    31441 caatgaactc cgtgttctgg tttgacgtag caggaatcaa tgatattggt aaatttttgg
    31501 cttctgaagg aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc ccaatcatga
    31561 tgttcgggct tccagcagga gcatatgcta tttacagaaa tgcacgtcca gaaaacaaag
    31621 caaaaacagc ttccttgatg ctagcagcag cttttgcttc attctttact ggtgtgacag
    31681 aaccacttga attttcattt atgttcgttg cttggccatt atatgtcttg catgcaattt
    31741 tcactgggat ttctttagca gtgagtgcat tcttccattg gacagcagga tttgccttta
    31801 gtgccggatt tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat cagccattga
    31861 tgttgatcgt ccaaggatta gtaacagcag tgatttacta ctttggcttt aactttgcta
    31921 tcaaaaaatt ccatctgatg actcctggcc gcgaggaagc agatctttca gatgacgata
    31981 ctgcaacgac taattcagac aataaatatg cggcacaagc aagcaaaatc tacgctgcat
    32041 taggcggagc cgacaatgta acttcgatcg ataactgtac tacacgcctt cgtttgcaag
    32101 tcaaagatac aggcctgatc gatcaaaata aaatcaaagc cactggcgta cctggtatga
    32161 agatcatcga cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa ttcgttgcag
    32221 atgagatggc aaaactacat ggtggtgctg cagcacgacc tgcacaaaca aacaccgtgg
    32281 ttaaaacaga aacatttgcc ggtgaaacga tcgaacaaga tatttacgct atcgcaaatg
    32341 ggaaactgat tccaatcact gaagtttcag atgatgtatt cgcggaaaaa atgatgggtg
    32401 acggctatgc tgtattacca gaaaatggag aaatcttctc tccaatcgct ggtacgatca
    32461 caaacatttt cccaacgaaa catgctgtcg gaatccaaac agatgctggt atcgaagtct
    32521 tgcttcatat gggcatcaac actgttgact taaaaggcga accattcact ttatatgtag
    32581 aagaaggtca aaaagtcgca agaggtcaat tgattgcttt agttgatctt gccgctattc
    32641 aatctgcagg caaaaacacg gatatggtcg ttgtattcac aaatggcgac aaagtccaaa
    32701 gcttagaaat cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc tctgtttcca
    32761 acaaagcctg agccatcgtc ttccccagac ttttttatcg ataaacaaac aggagctgag
    32821 acaaaaatct tgtctcagct cctttttccg aataaaaacg gcgttctcct gccaactccg
    32881 cggtaataag tccagctaaa cgaaacatat gaagagctat tttttatttg actcttctta
    32941 ttacttgatg cagaacagct ttttcacaaa atttttcttc atactcaaaa ctaatcggct
    33001 ttttcacaac ctctttatac attattcctg atccaatatt tcttttaatg ctgcttcaag
    33061 agaagttaat ggttttccca atactttttc taaatcatca gaggaaatag ctaactgatc
    33121 ctttttaata tcatgttgga tcgctaaaaa catgtcagct gcttcttgag ggaatccggc
    33181 ttcttttaga ttttcgacaa acgctgcatc acttgcttca ataatttcta actctttacc
    33241 tgtcgcacgc tctaatgcct tggctaattc ttcatagtca gttagattgc cggataattc
    33301 taagatttct ggagagtcac tttctactag tacttttgct gcagcttctg catattcacg
    33361 tttcaatgcc caacctgttt ttccatttcc tgcagcataa acgaattgac cagtttttaa
    33421 agcctgccca atgataggta attcattctc tacataccaa ttattccgta aaaatgtatg
    33481 ggaaatacct gaatccttaa tcattttttc agtgaattgg tgatctggcg ctaatacact
    33541 gatcgagtga tcggcatctg caaaacttgt ataagcgata taagataccc cagctgcttg
    33601 tgctgccttc actacattct tatgttcggc ttgacgattt cctggaacgc cagagacaaa
    33661 aagcaatcga tctattcctt tcaacgcttc tttcattgag tctaaatcat cataatcacc
    33721 aatacgtata ttaaatccag cttctttcag tgcttctcct ttttcttcac tacgtactag
    33781 tacatagatt tcattttgtg gaaccatctt ttttaagaat tccagcgcat aaccaccaaa
    33841 acctccagtt gctcctgtta ctaaataatg catatttgcc actccctatt ctttttattt
    33901 gttaagattt acagctgtac atttataaat tacaccttta tcataaagag tgtggtaaga
    33961 taagtcaagc aaaaaaaatg aaaggatgac aactgatgaa gtattctctg caatttagtg
    34021 atgcaatcca tgtacttgcc tatattgaaa tgtttcaaga tacccgatca ctttctagtg
    34081 agatgattgc cagtagtatc gaaacaaatg cagcaaatgt acgtaaaatc atgagtcagc
    34141 tcaaaaaatc cggtttgatc tttacaaaaa ccggaaaacc gcagccttcc cttgccaaaa
    34201 gtccagatca gatttcttta ctagatgtct atcgcagcat cgaaggtaat acacagctta
    34261 tccaagtcga tccaaaaaca aatccaaatt gcgtcgttgg tgcgaatatc caagaagctt
    34321 tagaagaaag ctacgctcgg ctgcagaaaa aggcagaaga agaaatggct aagatcacac
    34381 ttgatagtct gatacataaa atagccgttt tggaaaaaga aaaaagacca gaaaacacag
    34441 gcctcgtaga gagattttta taattctctc tactgtgcct gcatttttct gatcggaatc
    34501 gtctacataa attcttttat tctccggggg acaaaagcct tgtcatgccc ctttttcctc
    34561 tctaatgctc aggtcaaagc gctgaagtca tgacgtcttg ataaacggtg ttcaagtagc
    34621 tgaccctcgg cattagttct gaaaaatgca aaatatggaa agctatttcc catttttcct
    34681 ctctaatgct caggtcaaag cgctactttc acgacctcta tattatcatg gtttattgac
    34741 taccggtatc cagatttctc ccatatattt cccgttggta actcccattt cgactacagc
    34801 atttggaccc ccaacatagg cgtaatcttc tacttctctt aaaacatgcc caaaggccgt
    34861 attcgtcaaa tcatttctta attcttcagg cgattctgct aaagcgctta caacaagata
    34921 cgttcctttt gggaatggga tgattcgcgt agtgtttgga acatcctgac tcgtttgaac
    34981 accgacatag tacatcattt tcccgttata gggttcgttg actgtaaaga aataatgatt
    35041 ttcagcagct tgtttcaatg catctattgt tccattaccg ataacttttt gctcaaatgc
    35101 ttgtttctct gcagccaaac cgacaaaatc cgtattgcta ctcttcaatt ccacaccaat
    35161 acccgtcacc gtaaaatctg gtttttcttc gatcctatac tttgccattt ctcccaccct
    35221 ttctattata ttaagttcat cttacataaa attaaaggga aatgacacga ttacgctctt
    35281 ttttctaggg atatttttat tttttaacta tttttttgct ttcactgcaa tccaaatctc
    35341 actgtaaaca ttactgaaat cctctcccca ttttgtaaaa gagatctcgg gagcttcgac
    35401 taacccataa tctgaagaag gaagccattc tgcatagatt ttcccccaag tatcttgtaa
    35461 agtttgggga aatggccctt tattaggaaa aatggcccaa gtgtgttctt ctatagcaag
    35521 catttctaaa tcagcgtacg gattttcttt tgtcgtagca aagccaatca tgtgcatcaa
    35581 actgcccttt tcttccaagc ggtctccatc aaatccataa gaaacgttga ttacttgatt
    35641 tggatataaa tctcctaact tatgcatctc ttccctttga gtctccgtga tcgtctgcgc
    35701 taacttttgt atttcttgat tgactccttc aaatttgaca ggaacttttt tcgccacacc
    35761 tgccacataa aactgttctt tcttttcgat acgaaattcc atcgattttc ctcctctgac
    35821 atcaataaaa aatgtcaagc gtggaaaaga tttttgtatg ccgtatctac tgatttctga
    35881 aggcagatat cctgaccaat cacgaaaagc tcttgaaaaa ccttcgactg tttcatatcc
    35941 atatctaaac gccgtttctg tcactcttga tccatctaga agatcgcgat tagccaaagc
    36001 caatctacgg tttttgatat agtccattag cgacatccct gcaataaacg taaagatgcg
    36061 ttttaattga tattcagaga ttcctgtatt ttcggctatt attttatagg aaagtccttc
    36121 tgtcaaatgt tcttcgatgt aatcgatcaa ttggttcaga tctttaagca tatttatctt
    36181 cctccttgat tgccttttta tcatattatc aaagaaagtc aattacttga tgaatcagga
    36241 accaatttag tcagatgtag aaaatgttgt atggatctaa agaaaattct caagcagcgt
    36301 ttcgtaacgg ataattggta ttatctgcct atctatgttt tacctttaga aaaataaatt
    36361 ttggggaaga cagattctaa gtaaacaaag cggtaaaaat atcttttatt cccttagtta
    36421 atccgcttac tacctactgt acaaagcaaa ttgttgttca aactgctgat cagaacgctg
    36481 cttggatttt tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt
    36541 ctaacaaaat cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa
    36601 aacacgctat tctttta
//
LOCUS       DAZKNO010000005        35599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000005 DAZKNO010000000
VERSION     DAZKNO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.005"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..193
                     /locus_tag="ACG8MY_000177"
     CDS             <1..193
                     /locus_tag="ACG8MY_000177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429414.1"
                     /translation="RKCFNLITKKLIVCRMKETIYWVDESPRIVKRRFQDMPKMKKII
                     LAVMLFSTVISFTAPFLRT"
     gene            246..1157
                     /locus_tag="ACG8MY_000178"
     CDS             246..1157
                     /locus_tag="ACG8MY_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0429415.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1413..2330)
                     /gene="rnhC"
                     /locus_tag="ACG8MY_000179"
     CDS             complement(1413..2330)
                     /gene="rnhC"
                     /locus_tag="ACG8MY_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0429416.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            2532..2969
                     /gene="zapA"
                     /locus_tag="ACG8MY_000180"
     CDS             2532..2969
                     /gene="zapA"
                     /locus_tag="ACG8MY_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0429417.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            3027..3575
                     /locus_tag="ACG8MY_000181"
     CDS             3027..3575
                     /locus_tag="ACG8MY_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0429418.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            3690..6050
                     /locus_tag="ACG8MY_000182"
     CDS             3690..6050
                     /locus_tag="ACG8MY_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0429419.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            6137..6451
                     /gene="trxA"
                     /locus_tag="ACG8MY_000183"
     CDS             6137..6451
                     /gene="trxA"
                     /locus_tag="ACG8MY_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0429420.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            6633..8411
                     /gene="uvrC"
                     /locus_tag="ACG8MY_000184"
     CDS             6633..8411
                     /gene="uvrC"
                     /locus_tag="ACG8MY_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0429421.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            8556..9455
                     /locus_tag="ACG8MY_000185"
     CDS             8556..9455
                     /locus_tag="ACG8MY_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429422.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            9448..10710
                     /locus_tag="ACG8MY_000186"
     CDS             9448..10710
                     /locus_tag="ACG8MY_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429423.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            10871..12769
                     /locus_tag="ACG8MY_000187"
     CDS             10871..12769
                     /locus_tag="ACG8MY_000187"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0429424.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(13068..13787)
                     /locus_tag="ACG8MY_000188"
     CDS             complement(13068..13787)
                     /locus_tag="ACG8MY_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0429425.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    13977..14024
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            14124..14456
                     /locus_tag="ACG8MY_000189"
     CDS             14124..14456
                     /locus_tag="ACG8MY_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429426.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            14443..16446
                     /locus_tag="ACG8MY_000190"
     CDS             14443..16446
                     /locus_tag="ACG8MY_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0429427.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            16465..16935
                     /locus_tag="ACG8MY_000191"
     CDS             16465..16935
                     /locus_tag="ACG8MY_000191"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0429428.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            16956..17540
                     /locus_tag="ACG8MY_000192"
     CDS             16956..17540
                     /locus_tag="ACG8MY_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFQ0429429.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            17555..18541
                     /locus_tag="ACG8MY_000193"
     CDS             17555..18541
                     /locus_tag="ACG8MY_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0429430.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            18531..18842
                     /locus_tag="ACG8MY_000194"
     CDS             18531..18842
                     /locus_tag="ACG8MY_000194"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0429431.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            18864..20645
                     /locus_tag="ACG8MY_000195"
     CDS             18864..20645
                     /locus_tag="ACG8MY_000195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0429432.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            20638..22014
                     /locus_tag="ACG8MY_000196"
     CDS             20638..22014
                     /locus_tag="ACG8MY_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0429433.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            22021..22656
                     /locus_tag="ACG8MY_000197"
     CDS             22021..22656
                     /locus_tag="ACG8MY_000197"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0429434.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            22686..24041
                     /locus_tag="ACG8MY_000198"
     CDS             22686..24041
                     /locus_tag="ACG8MY_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0429435.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(24233..25627)
                     /locus_tag="ACG8MY_000199"
     CDS             complement(24233..25627)
                     /locus_tag="ACG8MY_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0429436.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            25719..26690
                     /locus_tag="ACG8MY_000200"
     CDS             25719..26690
                     /locus_tag="ACG8MY_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0429437.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            26687..27468
                     /locus_tag="ACG8MY_000201"
                     /pseudo
     CDS             26687..27468
                     /locus_tag="ACG8MY_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(27637..28008)
                     /locus_tag="ACG8MY_000202"
     CDS             complement(27637..28008)
                     /locus_tag="ACG8MY_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0429438.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TTPSYYFVIDPDGYKIEVIRG"
     gene            28142..28585
                     /locus_tag="ACG8MY_000203"
     CDS             28142..28585
                     /locus_tag="ACG8MY_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0429439.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(28628..29437)
                     /gene="yidA"
                     /locus_tag="ACG8MY_000204"
     CDS             complement(28628..29437)
                     /gene="yidA"
                     /locus_tag="ACG8MY_000204"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0429440.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(29456..30823)
                     /locus_tag="ACG8MY_000205"
     CDS             complement(29456..30823)
                     /locus_tag="ACG8MY_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0429441.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(30807..31637)
                     /locus_tag="ACG8MY_000206"
     CDS             complement(30807..31637)
                     /locus_tag="ACG8MY_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0429442.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            31800..32237
                     /locus_tag="ACG8MY_000207"
     CDS             31800..32237
                     /locus_tag="ACG8MY_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0429443.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            32313..32933
                     /gene="rpoE"
                     /locus_tag="ACG8MY_000208"
     CDS             32313..32933
                     /gene="rpoE"
                     /locus_tag="ACG8MY_000208"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0429444.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            33122..33628
                     /locus_tag="ACG8MY_000209"
     CDS             33122..33628
                     /locus_tag="ACG8MY_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0429445.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            33645..34544
                     /locus_tag="ACG8MY_000210"
     CDS             33645..34544
                     /locus_tag="ACG8MY_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0429446.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            34631..35516
                     /locus_tag="ACG8MY_000211"
                     /pseudo
     CDS             34631..35516
                     /locus_tag="ACG8MY_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
BASE COUNT        11700 a         6143 c         7599 g        10157 t
ORIGIN      
        1 caggaagtgt tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat
       61 ttattgggta gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat
      121 gaaaaaaatc attttagcag taatgttatt ttctacggtt atatctttta cagccccttt
      181 tttgcgtact taagctgatt ttaagttttg acccttagtc tggatctacg aggtgagagt
      241 caagaatgaa aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt
      301 ttcaaataga tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca
      361 gtacggttac aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa
      421 tcggaaaaaa tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta
      481 taaacggcgg agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga
      541 caaatgatgg ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata
      601 tacaaacaaa accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg
      661 gaaatgatac tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa
      721 gtacagccat aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc
      781 atactacaga tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg
      841 cagatgtctt tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa
      901 tcacgacata ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct
      961 tcactgatct gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag
     1021 aaaatcttca accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa
     1081 aggaattagt aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga
     1141 ccacttattt tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg
     1201 tttggccggg cttattatcg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt
     1261 gagcctgccg tttaactccg cgtcacaagg agcaatttca aaaaacagct tctcatgttt
     1321 gatgactatt gtgacttgtg accgaggagt tggcatgaga acaccgttta ttcggaacaa
     1381 gggactgtga catgactttt gtcacagttc cattattttt ttagcagctt caaagctttt
     1441 tctgtattgg caaaatgaag tttggcatat ttttcaagca taggcatgcc gcctttctcc
     1501 aatagttttg ccgctacttg gtcagatttc gttccagctc ctgatggaag ggtgattcca
     1561 gcttcagctg atgctttgtg taactcttcc aaaaagctgg cacgactgat gatagaagca
     1621 gcagcaacag ctaaatgata ttgttctcct ttagtaacga agtacagttt ttcttgtaat
     1681 tgattttttt ctaaacgcgc atatttttta aaatttgctt ccggcgtaaa ctgatcgatc
     1741 aaaatcgctt ctggtttttc tggctcgatt ttttttagca gcaaatggat cgcttgattg
     1801 tgcaaagcga ctttcatccg aacggcattg tattttggtt ggatctcatt gtatttttct
     1861 ggagataaga caagcagttt ataggggatg gtttttttta agatgtcagc aagctggatg
     1921 atttggctat cttttaactc ttttgaatct ctaacaccaa gtttacgtaa ttctgcaagc
     1981 tgatcacgtt tcacataagc agcacatact gttagcggac caaaatagct gccatttccg
     2041 acctcatcac tacctaaaac ggataattga ctgatatttt ttggcaaaga agaggctgtt
     2101 tttgctggct tggcttgttt atcctgtgcc tccccccatt tatcagcttc tattgctgca
     2161 ttcgtacctt gaaacatcac ttttccggat gtataagcgg taatggtcgt cccgtctttt
     2221 tttgcagcaa agctagtata aggaatctgt ttatttaaca aataagagcg atagtatgct
     2281 ttcatttgac taagttggtc gtttgttgct ttgataatga cgttgctcat aaaattctcc
     2341 tcgtttttat acataaaact ataccgcaac tcacgacttt tcgttttcta agagattgcg
     2401 agacatagca tttttttaaa gtttatcata taatagttaa gtatgtgaaa aatatttgtg
     2461 gagaacggtg aaagaaccaa cttcatggta cactaaataa aactttcatc aaataggagg
     2521 aaatgattcg tatggcgcac gaaaaaacac gctacaaagc agtgattgcc aaccaaacat
     2581 atactattat cggacgggaa accaagcatc atatggatat cgtaacgaag ctgatcaatg
     2641 aacagttagc agaattgaaa caattatcac cacaaatgga taatgaacaa gcagcaatct
     2701 tgatggctgt caatgcgctt tcggatcaat tgaagaaaca agaaagaatc ttggagctgg
     2761 aagaagaaac agcagaatta aagaaaaaaa tgatcaaatt tacagagctt gaaaatcgtg
     2821 tgaaacgcat tgaagcaatc gagaacgaag ctcgggaagt attgaaagaa aatggtcaag
     2881 cagatcatac cattaaaaat catgtggaag cacaacagat tttaaacgag aaacgaaaag
     2941 gacaaattaa acaaaaatct tctaattaag atccttttat caaaataaga aaaatttaaa
     3001 aaagttagaa gagaaaggaa cgtagcatgc tgagtttgct gattttattt ttattgctaa
     3061 ttgcgttttt ttctggagca cgtcgcgggt ttgctttgca aatcatattc gcgattggat
     3121 atgtattgtc atttattgct gcccagcatt tttacaaacc attagcaagt catttggaat
     3181 tatacattcc atacccagct gtcaccccaa catcaaagtt agcatttttt gatcaggtat
     3241 ttgcttttca cctagatgaa gctttttatg caggaactgc ttttctgatt attttgctaa
     3301 ttggctggct gcttacccga tttgtggggg tttttgtcca tggattgacg tatgtgccga
     3361 tcttgagaca ggtagactgg attgcaggag gcatcctgtc actaatcatg gcttacgtta
     3421 ctatattttt gattttgcag ttacttgcct ttgttccatt agatgtggtc caaaatcaat
     3481 tcggcgagca tagtttcgct cgttttatcg ttgagagaac gccatttctt accaacaaga
     3541 tgtatgagct ttgggtaacg aatattttga attaaggaaa atagtgctgg aaaatatttc
     3601 gttttttgta cagagtagtt tatttaagaa ggctgcgtag cgtatgtccg gcacagctct
     3661 tttttcatag tgaaagataa ggtgataaaa tgaataaccg aatccttgaa acattggaat
     3721 ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac tgcacaagga aaagaagaac
     3781 tggcagaatt ggtaccagtg agtgagaaac aaacgatcac aaactggctt caagaaactg
     3841 aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc tatacctaaa cttgaaaata
     3901 tccgtcccca tatgaaacgg atcgagatcg gagcagactt gaatggagtg gagcttgcac
     3961 aagtttccag agtattgtct acgacaagcg aattgaatcg tttcattgat gatctttctg
     4021 acagtgaaat cgaatttgca cgtttgtata tgtgggcaga tcagttagtc acgatccctg
     4081 ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg acgcgtgact gatgacgctt
     4141 ctcctgagtt aaagagcata agacagaaca ttcggcgaag tgaacaagct gttcgtgaac
     4201 agttagatgg gatcgtccgt gggaaaaatg caaagtattt aagtgatgcg atcatcacca
     4261 tgcgaaatga ccgctacgtt atcccagtca aacaagaata ccgcggtgta tttggcggtg
     4321 tagtccacga tcaaagtgct tcaggacaga ctttatttat tgaaccaaaa caagtggtgg
     4381 atttgaataa tcgtttgcgt caataccaaa tcgctgaacg aaatgagatt caacgtattt
     4441 taagtgaact ttctgctgaa cttgttcccc atcgtcaaga aatcatccac aatgcttatg
     4501 tgattggtaa aatggatctg atgaatgcaa aagcacgatt tggtaaagaa gtaaaagcca
     4561 tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa acaagcaaga catccactga
     4621 tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg aaaagactat caagccatcg
     4681 tcattacagg acctaacaca ggtgggaaaa cgatcacgtt gaagacttta ggtctgttac
     4741 aactgatggg gcaagcagga ttaccgattc ctgcaggtga agaaagtcaa atcggtattt
     4801 ttgaggaagt atttgcggat ataggagacg aacagtcgat tgagcaaagt ctatcgacct
     4861 tttcttctca tatgacgaac actgtagata ttttatcaaa agtcaatgaa aaaagtctgg
     4921 tattatttga cgagctcggt gcaggaacag atccgcaaga aggtgctgct ctagcaatcg
     4981 caatcttaga tgatcttggt aaaaagtctg cttatgtgat ggcaacaacg cattatcctg
     5041 aattgaaagt ttatggatac aatcgggcaa atacgattaa tgccagcatg gagttcgatg
     5101 tggatacact aagtccaacg tatcgtttgc tgattggcgt tcctggacga agcaatgcgt
     5161 ttgaaatctc tagccgatta ggtcttgata cagaagttat tgatgaagcg aagcagttga
     5221 tgaatgatga gagccaagat ttaaacgaaa tgatcacaga tttagagaat cgccggaaaa
     5281 tggcagagac cgaatatctg gaaatgcgtc attttgtttc tgaggcacaa gaacttcacg
     5341 atgatttgaa agaagcttac agctatttct ttgaagaacg tgaaaaagaa atggaaaaag
     5401 caaagaaaaa agcaaacgaa gtcgtgtcag aggcggaaga aaaagcagaa aaaatcattg
     5461 ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa tgtgaaagag catcagttga
     5521 tcgatgcaaa gacccaatta gcaaatctac atcaagaaga aactttgaag aaaaataaag
     5581 tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg agacgaagtg ttggttacga
     5641 cttacggcca aagagggacg cttttacgta aaaacgggaa tcagtggcaa gtagaaatcg
     5701 gtatattaaa aatgaatgtg tccgaagacg agctgacgcc tgttgctcct caaaaagagc
     5761 cgacacaacg agttatccat gctgtacgat ctgaatctag tagtcacgtg ccgaatcaat
     5821 tggaccttcg aggaaaacga tacgaagaag cgctaagtga ggtagatcaa tatttggatt
     5881 ctgccattct agctggttat cctcaagtga ctattgtcca cggaaaagga acaggcgcac
     5941 ttcgcaaggg aattactgat tatcttaaaa accatcggag cgtcaaaagc tttgagtttg
     6001 caccggcaaa ccaaggcgga aatggtgcga caattgttaa atttaagtaa agcttactaa
     6061 aggtaagcag atggttcgct atccaagtat agaaatgcta tactaatcat agattttaaa
     6121 aatggaggtc tttgatatgt cacaagtaat tacagataag acatttaatg aagaaacaga
     6181 taaaggactt gtattgattg atttttgggc aacttggtgt ggtccatgtc gtatgcaagc
     6241 accgattttg gatcagttgg aacaagaata tgatgaagaa gaattcagaa ttgctaaaat
     6301 ggatgtggat gaaaatccag aaactcctca acaattcggt atcatgagca ttccaacatt
     6361 aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt ggtgttcatt caaaagaaca
     6421 actacgtcaa atgatcgatc aatatttata aaaacttaca gcaaaaataa gctgagggtg
     6481 ggactgaatc cagttccgtc ctcggttttt ttgcggttga gttaaggtta ttcaaattaa
     6541 tattttttga gtataattac gttgtcaggc tggattgttt ttttgacaga catgctataa
     6601 taactttaat tgaatatgaa ggggagtacc agatgaacga acgaatcaaa aacaaattag
     6661 ctttacttcc tgatcagccc ggctgttact tgatgaaaga taaaaatgga acgatcattt
     6721 atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc atattttact ggcagccata
     6781 atacgaaaac ggaacggctt gttagtgaaa tcgaagactt tgaatacatc gttacagaat
     6841 cgaatatcga agcattgctc ttagagatca atctgataaa gaaaaatgat cctaaataca
     6901 acattatgtt aaaggatgat aaaacctatc ctttccttaa gataacgaat gaaaaatatc
     6961 cccgtttggt gattactcgt aaagtactaa aagacaaagc actgtatttt ggaccatatc
     7021 ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga tcgattattt cctctgagaa
     7081 aatgcaaacc tagtcagacg aaggagcctt gtctttacta tcatttggga caatgtctgt
     7141 gtccttatta tttcgacgta gatccgaaag tctatacgaa aatcgttgat gaagtgaaaa
     7201 agtttttaaa cggaggacac gaaaaaatcg agtcagagat ccatcagaaa atggaaaaag
     7261 ctgctgaaaa catggagttt gaaaaagctg cagaataccg agaccagatc cgtgccattg
     7321 agacaatcat gactcgccaa aaaatgacaa atactgattt actggatcga gatgtatttg
     7381 gttatgcgat tgacaaagga tggatgtgtg tccaagtgtt tttcgtaaga caaggaaagc
     7441 taattgaacg agatgtttca atgtttcctt tctataacga agcagaagaa gatttcttga
     7501 cttacctcgg tcagttttat caggaaaatg agcatttcat tcctaaagaa gtcttgattc
     7561 ctgatacgat cgacaagccc agtgtagaag ctttgttagc tacgaaagtg ctgcaaccaa
     7621 aacgaggaga aaagaaaaaa ttagttgaat tggcgaataa gaatgcctca gttgctttga
     7681 atgaacggtt tgatctgatt gccagaaaac aggaacggac gatcggagca gttgaacgtt
     7741 taggaaacgc gatgaatatc ccgaccccaa tacgtatcga gtcttttgat aactctaata
     7801 tcatggggac gaatcctgtg gctgcgatgg tcgtattcat tgatggaaaa ccggcaagaa
     7861 aagaatatcg gaaatataaa ataaaaacag tcaacggacc ggatgattat gcatcaatga
     7921 gagaagtcat ctatcgcaga tattcacgag tgattcgcga agggttgcct ttacctgatt
     7981 tgatcttgat cgatggagga aaaggacaag tagatgtggc aaaagatgtc ttagaaaatc
     8041 aattaggaat cgatattcct attgctggca tggcaaaaaa cgataaacat aaaacaaatg
     8101 agttgctatt cggtatagat ttggctgtag taccgcttga gcgaaattca ccagagtttt
     8161 tcttactcca gcgaatccaa gatgaagtcc atcgattcgc aatcactttc catcggcagc
     8221 tacgcagtaa aaacagtttt gcttcccgat tagatgaaat tgaagggtta ggaccaaaac
     8281 gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa tatcacagca gccacgacag
     8341 aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca aaacgtattt gataaattac
     8401 atcaggaatg aggaaagggc actttacaaa gaaagtaaag tgtcctatca ttttatgtac
     8461 aacaaaaaga gcgtttacgt tttgttggta aataataaat cgcttacgtc tggtgtctaa
     8521 ggatatatgt gatttcttga agaaggagtg aagaaatgtt agaagtcaag gatcttgtta
     8581 aaacttttgg gaactacaca gctgtcgatc atgtctcttt ccaaatcccc gatggaaaaa
     8641 tcatggggct aattggccaa aacggggctg ggaagacaac gacttttcgt ttgatcttgg
     8701 attttttgac acaagaccaa ggagaagtgt tatggaatgg ccatcatctt ggggagaaag
     8761 attatgatgt catcggttat cttccggagg aacggggact ttatccgaaa gtaacgattc
     8821 aagaccaact gctttatttt gcagaactta gagggaaatc aagaaaagag attgaaccaa
     8881 aaatcgattt ttggatggaa aaatttcagg taaaagggaa aaagacggat aaagtaaaat
     8941 ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc cacgttaatc catgaaccta
     9001 aattgatcat tttggatgag ccattcagcg ggttagatcc ggtaaatgct gaattgctaa
     9061 aagatggaat attggaattg aaaaaacatg gttcatgtgt cattttttct agccacaata
     9121 tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt gcgaaacgga aaaatggttt
     9181 tgaatggaaa agtacatgag attcgtgaat catttggccg tacgaagtta ttcttggaat
     9241 ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt gaaaaaagtg atccttcatg
     9301 aagacaatac gttagagatt accttagaag atcctgaagc tgggaaagaa atctttactc
     9361 gtgcgactca gttcggctac attcctatgt ttaaccagca accgccaact ttggaagaga
     9421 ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt ggattattac caaagatgtc
     9481 tatttaaaaa atgttaagtc gatttctttc ttgatcatga tattagtccc ttttatcatg
     9541 ctgggtatca tttatgtagc aggaaacttt gctcaacaaa atagtgatgt caataaaatc
     9601 ggcgtattgg ctgataatga gcaaatcagt cagcaaatga gccaagtcaa aacagatgat
     9661 tttgagttca aagcagttga ttctgaagaa aaagcacaaa aagagctttc ggaagaaaaa
     9721 atcgatgcgt atttagtagt ctcaactgag aatgataaga taaatgggat gctttatagc
     9781 gaaaattcat taggacaaag tacacaacta ctgatacaac aacaactgac tggcttgcaa
     9841 tcgatgcttc gagcaagtca attaggcatt tcaccagaag aagtagctag cttgagccaa
     9901 caagcaagtt tgactcgaca aaaagtcaat tttgattcaa aaggaaaaat gacagttgga
     9961 gaagataact cgacagtcca atatgtcgta agttatgtag caacgattgt cttgttcatt
    10021 attattttga cgtatgcgca gatcattgca caagagatcg cttctgaaaa aggcacgcgg
    10081 atcatggaag tgatcctgtc cagtacgact gcacaaaagc atttttacgg aaaactaaca
    10141 ggtgttttac ttgttgcatt aacgcaaatg gcattatacg gtattatttt tggtatagga
    10201 tacagtcaat tcaaagatat ggaaatggtc aaaaatatat tagacggtat ctcattggat
    10261 aacttgattg gaccattctt gtggtattct cttctattca tgatctttgg cattttgatt
    10321 ttttctgttt tagcagcttt atgtggctct ttggtcaaca aagcagaaga tacagcaaaa
    10381 gcaattttgc cagtgactta tctatctcta ggcggatata tgcttggatt gatactagga
    10441 gcttcagatc caaacaatat cgttattcgg atcacttcgt atattccatt cttatcttca
    10501 tatattatgc cggttcgctt agcaaatgag acagtcggta tgggtggagc attgatttcc
    10561 ttgttggtac ttgtagccgt aacattctta ttgatgatct tgtctgcaaa catgtacaaa
    10621 tcgaatgtac tcgtgtatag cgaaggcgga ttatggagtt cattgaaaca atcgatttca
    10681 attatgagaa atgaacgaaa aaaagcataa aatatagaaa aatatagaag gaagctggac
    10741 atttattgtt cccagcttct tttttgtata gacctatttc tattttttta ttatattgga
    10801 tatatattca ttgaaaatag agcataacaa ctttataatg ttgagtatac caatttttgg
    10861 aggcgtttta atggaccgct atttagattt actttcagag aagttcccta caacggaaga
    10921 tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag gaacagagtt
    10981 atttttgagc gatatacatg gcgaatttcc tgcttttgat catattctgc gtatcggttc
    11041 aggaaatctt aaagaaaaga tcaaagacct tttctctgaa caaatgacgg aaacggagat
    11101 cagccaattc actattttta cggcttatcc agaatatgtg ttgacgactg attggtacaa
    11161 aaaacaagat aaatcccagc tgatccatcg tttgatcgat cttttacggt ttacgacagt
    11221 caaatacact cgatcaaaag ttcgaaaagg attgccgaaa gaatatagct atatcattga
    11281 ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa gactatgtaa aaaaaatcat
    11341 tgaacaactt gtcttgatga aggaagaaga aaaattttta acaaaacttg cgcagacgat
    11401 ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat atttttgatc gaggaactca
    11461 agcagccaaa gtaatggatc gaatcatgga tttttcctct gtagatatcc agtgggggaa
    11521 tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttat tgacgctttt
    11581 acggattgcc gcacgctaca actatttatt tgaattagag aaagaatatg gattaaattt
    11641 acgtccatta ttttcttttg cccatcaaac ctatcagaag aatccagtat ttacccctaa
    11701 aaacaggaaa gatctatctg aaagagagca agaagaatta gaaaagatcc atcaagcact
    11761 ttctatcctc caattcaaat cagaacatca gttgttgaat cgacgacctg aatttaaaat
    11821 ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa tcaacgatcg ttttagaagg
    11881 tcagctctat gagttgaaag ggacttgttt tcaaacgatc cagccagatg atcctaaaaa
    11941 attcttggaa gaggaacaaa aagtgatcga tagtttgatg tattcattcc agcactcgtt
    12001 aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg ggaatgtatc tcacgaacaa
    12061 tcagcatgta ctgttccatg gctgtatacc tgtagatgaa aaggggcagc tcttagcatt
    12121 tgaattagac caatcttatc gcggaaaaga actgctggga ttttttgaaa aacagataac
    12181 tgaaagcgca aaagatttga ctgcaaatga agatttggcg accgatttga tttggtatgc
    12241 gtggagcggg ccattttcgc ctttattcgg caagagtaaa atggcaactt ttgagcgcta
    12301 ttttctcaca gagcctcata cgcacgtcga aaaaagcaat ccctattatc acttgcgaga
    12361 tgaagagtgg ttcagtgaaa aaattttggc agaattcgat gcaaaagaag aaggttcagc
    12421 aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga gagtcaccga tcaaagcaaa
    12481 cggaaaagca tttgttattg acggaggtct atccaaagct tatcaaaaaa caaccgggat
    12541 tgctggctac tcattactat gcaattctta cggtttccaa atcgtgacac attatccgtt
    12601 cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa acgaatgtaa aaaaagtgat
    12661 cgagcagcag ctcccacgca aacttaatcg gaatacgaca aaaggaatgg aattacagca
    12721 acagattact caactccaaa ggctgttgga ttatcgaaag gacgattgag aaaaatgcta
    12781 gccaacattg ctcaatagac taaaaagcga ctgcaacaaa aggcgtgtcg cagtctcctt
    12841 cggaattagc tcaaaaaacg gtaaaatatt agaagctatt tttctgttta tttctctaat
    12901 actcaggtca gggcgctgat ttcacgacct cttccttaaa cggtgttctc ctgccaactc
    12961 ctcggtcaca agtcacaata tccaacaaac atgagaagct gttttttgaa attgctcctt
    13021 gtgactcaga gttgaacggc aggctcacga cctaatcaga aaaacgatta aaattcattc
    13081 acaaagaaat cgaataaacg ctgttcagat gaatctacac gatgagaaag ttccggatgc
    13141 cattggacac caaggatttt gtgcatatta tctttacttt ggactgcttc aatgagtcca
    13201 tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat ctttgactgc ctgatgatga
    13261 taggaattca caaagtatga atcagggaga aggtcactta ataaactgtc cgaaacgatt
    13321 ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt gttcgtgttt tacggaccat
    13381 tttggatata atgaaagatc ttgataaagt gttccctcca acacgacatt caatagttga
    13441 agtcctcggc agactgcaaa aatcggtttt ctttgtttca atgcttccaa gatcaatgcc
    13501 tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt ttggatgagg gtcttccatg
    13561 taaagttgcg gactaacatc ctgaccgcct gcaagaatga gtttgtcgat tttctcaatt
    13621 agttgaggcg ctgaatctgg agacatgact ggcagaataa gaggtaatcc ttgagcatct
    13681 aaaactgcat ccacaaatcc ctgcggtgta tagcttactt gatttccttg gaaagtatct
    13741 gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg gtttcatttt tattccccct
    13801 aaagtagact aattgtaaca aggatttaaa aaaatttaaa ctatgtaaga cttgccccta
    13861 tacttatctg ttcttgctgt gttactatct gtaaagagaa ttttcgtgtg aaattcatga
    13921 cgatagttga aaatattgct taatttgtta taataaaaag taagcagaaa taaaaaggtt
    13981 gggcgtaagc ttcaaggatc tagttcttca aggggtgaga aggacatttt attgaagggg
    14041 cctgcctata ctaagttatc tttagaggta catactttca atgaaaaaag aaagcatgta
    14101 cctctttttt aaatcgaaag gaagtgaagc aagtgttaga agctttggaa caaatccgaa
    14161 aagcagaaga agaaaatgag aagcgtgctc gaaccctacg gcagcagttg aagacctatg
    14221 cagaagaaaa agagcaagaa ttggagataa tccaaacgaa cagtcggcaa cagctgaaac
    14281 agttggtaga agaaaaagaa caattcgaaa agcaagaatt agaaaaagaa aaaatgaaat
    14341 tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga ccaatatcaa aagcatcaga
    14401 gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac ttatggccgt cactaagatg
    14461 gaaaaagtga ccctcatttc agataaaaga aatcaggaga ggattttaca agcagtacaa
    14521 ggaatccaaa atgttgaaat ccgggatctg tatcaaaata gaacaaacaa ccagtgggta
    14581 gaaaaatatt ttccagatac cacgattatt gataaagaag caaaattaag cgctttaggc
    14641 aatcggctga aagacatcca agaagctatc caattcatac aacaccacgg cgatagccgt
    14701 caaaagaaac tccatttgaa acgtcaagaa ctttccttac aagacttgga agcaaattat
    14761 tccgaagaaa atttcatgaa taaattagaa gaggtccttg atttaaaaga aaagtgggaa
    14821 cagctaactg aaaaacgtga tcgtttagaa gaagaggaag aatggcttct tcattggcaa
    14881 cagctggata ttgctccaag tatgtatgca agtaagacga ccatttttga gatggggact
    14941 gtgagtgcag caaattttga aagtttcaag gaagaactaa gtaagcttga tgacgtttat
    15001 atggaagaaa tagattatac gccaaaacaa gtttatcttt cgtatgttgc tttaaaaaaa
    15061 tcagttgctg ctatagaaga aattgctggg aaatacgggt tcaataaaga aacgtatcct
    15121 tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac agcaattatc cattgttcac
    15181 gagcagcaaa aaaagataac gacagcgtta ggtcagtgca gcggctatat ccaagaattt
    15241 gaatgggtag aagaagtcac acttgccgtt gcagaacggg aaaaagtaaa agaacggttt
    15301 atccatgcca cttatctgat cgttttgcaa ggttgggtag atgcagaaga aaaacagaat
    15361 ttgatgcatg tgttaaacga aactgtatca gaagaagata tatacgttag tttcgaaaca
    15421 ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca tccgcttatc
    15481 gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga agtagatcca
    15541 acgccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc agatgttggt
    15601 tacggattgc tgatgctcat tgggacaata ctagctcaaa aactatttgt tttaccccgc
    15661 gggatgaaac gtttcgtaaa attctttgag attctctcgg taccatcgat catctgggga
    15721 ttgatctaca gttcattttt cggacaggca ttaccaaaag aactatttgg tatccgcttg
    15781 cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt atccgtgatt
    15841 ttcggtctga tacagatctt ggttggatta ttcgtggcag caaaggaaaa tatcaggagg
    15901 aaagattact tgagtgctgt cagtgatggt tttgcctggc aagggatcct gattgggatc
    15961 gttattgcat tgattggtgt catgttgata aaaaatacag catttgttta tcttggcgcg
    16021 gggctatcga tcattgctgc tttatgtatc ttaatcgtgc cgattatcca atcgccgtct
    16081 aaagtcaagg gtgctgccaa aggagcgtat aatctttacg gattgactgg ttatatcggt
    16141 gatttagtca gttatacacg tttgatggca ctaggtattt caggcggaag tattggcgcg
    16201 gcattcaata tgctagttgc tttcatgccg ccagccgctc gattcagtgt agggatcctt
    16261 ttgatcatcg ctttacacgc actgaatatg tttttgacac tgttaagtgc ctatgtccat
    16321 ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt atacaggagg cggacgtgcc
    16381 tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca atcataagaa aaaaaacaat
    16441 aaataattgg aggattttta tacaatgatg gattacttaa ttagtcaaaa tggtggaatg
    16501 gtatttgcag tattagcgat ggcaacagcg acgatttttt cagggattgg ttcagctaaa
    16561 ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa caagtcaacc cgaaaaattc
    16621 ggacaggctt tgattctgca attgcttcca ggtacgcaag ggttgtatgg attcgttatc
    16681 gcctttttga tttttatcaa cttaggaaat gatatgtcag ttgtacaagg attgaacttt
    16741 ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt caggtatcgc ccaagggaaa
    16801 gttgcagcag caggtatcca aattttagcg aaaaaacctg aacatgctac aaaaggaatc
    16861 atttttgctg cgatggttga aacatatgcg attttaggtt tcgttatttc cttcctatta
    16921 gtattgaatg cctaagcata aaggaggaaa agcagatgaa cgccattgaa aaaatcatct
    16981 ctcagatgaa tgaagcagca gagcaagaaa gagcggcgtt agagcaagaa gagcgaatga
    17041 agatcgatca aaactttgaa caaaaacgta cgcaaatcga aacagaacat caaaaacaaa
    17101 aagaaaaaca aatcgaatta cttgaaaaaa aataccgtca gcttaggaat cgccaacaag
    17161 tagaagtacg acaagaaaat ttgaatgcga agcaggaatt tttacgacga ttgtttgcag
    17221 atgcaatagc tgaaatggag aattgggatg aatctgaaca aatccagttt atcaaaaatt
    17281 cattgtattc attacctttg acaggcaaag taacattcat tgctggagaa aaatcagcag
    17341 cttatctttc tcagacattg ctggatgaat ggaacaatga acttcccttt atgatggttt
    17401 taagtgatga aactgttgct gatcaagcgg gttttctgat aaacgatcaa ggagttcagt
    17461 ataattttct tttcagcagc cttgtacaag atatccaagg aacgatgagt tttgagattg
    17521 ccaatcaatt attcgaataa ggagggatca gtgtatggaa taccacgaac taaatccctt
    17581 gattcgcgga agagagcttg aacttttgtc caatgagaca tttgaacgga tgattcaaac
    17641 aaattctgtc gaagcacttg gtgaaatatt gaaatccacg atttacagtc catatattta
    17701 tgacggatat gaatatgact tcgaacagaa tcttttaaag gagcagagcc gtctatttcg
    17761 ctggttaaag gaatcagcac cggagcctga aatcgtttgg atctatacga tgcgctatac
    17821 tttccataat ctgaaggttt tgacaaaagc cgagatgaca aatcaaaatc tggaccattt
    17881 gtatatcgac gatggatttt attcattaga aaccttgaag gatgccattc atacgcaagt
    17941 ttcaaatgaa ctccctgaaa gcattatgga cagtatacga gaagtacgtg attattttga
    18001 agagtctacc attcttcagg gaattgacgt gatttatgat cgacagtttt tgacagagca
    18061 gcgacggctt gccgataagc taggatatca agaattgctg acagaagtca ttgcttttat
    18121 tgacttgacc aatatcacaa cgatggctcg aggaatattg cagaaaagaa catatgggtt
    18181 catgacgacg gtactttcca gctcgggaag tatcgaaaaa gaagtatttt tgaactttgt
    18241 agataaagat atggaagatt acacgaattt tctattagag acagattatg ctgattttct
    18301 gcgccctgtc atccgtgagg gaaatcttga tctcgtaaag cttgaacagt tgaaagatga
    18361 ttatctaagt tcattgtatc aagaagcaca aacccaggct ttcggaccac tgccactact
    18421 agcctttttg aacgcaaaag aggtagaaag caaaaacctt cgtttactgg ttatcggcaa
    18481 aagaagccat ttcgaaccag aaacaatcag aaagcgggtg agacaagtct atgacgcata
    18541 agatcggtgt agttggcgac aaagattctg ttttgccatt caaattgttt ggctttgatg
    18601 ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg gcaaagaatg
    18661 agtatggcgt gatttatgtc acagagcaat gtgcagcatt gagcccagaa acgatcgaac
    18721 gttacaagga acaaatgata cctgctatcg tattgattcc caatcatcaa ggatcattag
    18781 ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa aatatcttat
    18841 agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat cataaaagtc tccggacctc
    18901 ttgtaatggc tgagaacatg tctgatgcca gtatccaaga catgtgtcta gtgggagatc
    18961 taggagttat cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt caagtatatg
    19021 aagagacttc tggaattggt ccaggcgaac cggttcgctc tactggtgaa gctttatctg
    19081 ttgaattagg accagggatc atttcacaaa tgtttgacgg aatccaacgg ccattagata
    19141 cctttatgga aatcacacaa agtaatttct tagggcgcgg agttcaattg cccgcattgg
    19201 accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg gtgagtgctg
    19261 gagatgttat cgggatcgta gaagaaacaa aagttataaa acataaaatc atggtgccaa
    19321 atgggataaa aggtacgatc aaaacaatcg aaaaaggttc attcactatt gatgaaacag
    19381 tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa aaatggccag
    19441 taagacgtag tcggccaatc aaagaaaaat taaacccgga tgcacccatg atcactggac
    19501 agcgagtgat tgatacattc ttcccagtga caaaaggagg agcagcagct gtacctggtc
    19561 cttttggtgc agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc gatgtggaca
    19621 tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt ttaaatgaat
    19681 tccctgaatt gattgaccct aatacaggag aatcgttgat ggaacgtacg gtattgattg
    19741 ctaatacatc aaacatgcca gtagcagctc gagaagcttc tatctatacg ggaatcacaa
    19801 ttgcggaata tttccgtgac atgggttatg acgttgctat catggcagac tccacttcac
    19861 gatgggcaga agcacttcgt gagatgagcg gacgtttgga agaaatgcct ggtgatgaag
    19921 gttatccagc ttatcttggt tcacggcttg cggaatacta tgaacgatct ggaagagtca
    19981 ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct gtatcgccgt
    20041 ctggagggga tatttcggaa ccagtcacac aaaatacatt gcgggtcgta aaagtattct
    20101 ggggtcttga ttccagttta gcccaaaaaa gacattttcc ttcgatcgat tggatacaaa
    20161 gctattctct ttattcaaca gaagtaggga agtatatgga tcatatcctt caagctgact
    20221 gggcatccat ggtcaccgag ggcatgcgta ttttgcagga agaacagcag ctgaatgaaa
    20281 tcgtgcgttt ggtagggatt gattcccttt cagataatga ccgtctgaca ttagaagtgg
    20341 caaaatccat tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc gatacattca
    20401 cttcaagaga gaaacaattc aaaatgttga agatgatctt gacctttggt gaagaaggac
    20461 gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat ggacggtacc gtatcgattc
    20521 gtgaaaggat cggacgaagc aaatatatcc cggaaacaga attagaaaaa ctagatcaaa
    20581 ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc atgagcaatg
    20641 attaaagaat atcgtacgat cggtgaagtc gtcggtccat tgatggcggt tgaaaaagtt
    20701 gctggtgtca aatatgaaga attaatcgaa gtccgtatgc aaaatggcga gattcgtcgt
    20761 ggacaagtat tggaagtcca agaagataaa gcaatggtcc aaatattcga aggaacaagt
    20821 ggtatcaatt tacgtgactc ttctgtacga tttttaggcc atcctttaga actaggtgta
    20881 tcggaagaca tgatcggtcg agtattcgat ggtttgggac gaccgaaaga taacggacca
    20941 gagatcctgc ctgaaaagca tttggatatt aacggagaag taatcaatcc agtagctcgg
    21001 gattatccag atgagtttat ccaaacaggt atctctgcaa tcgatcattt gaatacatta
    21061 gttcgtgggc aaaaattacc agtattttct ggctctggtc ttcctcataa agaattagct
    21121 gctcagatcg cgcgacaagc aacggtactc gattcatcag atgatttcgc cgtagtcttt
    21181 gctgctattg gaatcacttt tgaagaagca gaatttttca tggaagattt tcgtcaaacg
    21241 ggcgcaatcg accgttctgt catgtttatg aatttagcaa acgaccctgc aatcgagcgt
    21301 atcgctactc ctcgtatggc tttgactgct gcagaatatc ttgcttatga aaagggaatg
    21361 catgttttgg ttatcatgac agacatgacc aactatgcag aggcattgcg agagatttct
    21421 gcagcacggc gagaagttcc aggaagaaga ggatatcctg ggtatcttta cacgaactta
    21481 gctactttat tcgaacgagc aggaagaatc cgcggactaa aaggatcggt tactcaaatt
    21541 ccgattctaa caatgccaga agacgataaa actcatccaa tccctgactt gactggttat
    21601 attacagaag gacagatcat cttgactaga gagctttaca agagtgggat tcagccacca
    21661 attgatgtac tcccgtctct ttcgcggtta aaagataagg gaactggacc cggaaagaca
    21721 agagaagacc atgcagctac gatgaatcag ctttttgcag cctatgcaca aggaaaacag
    21781 gcaaaagaat tggccgtagt tttaggtgaa tctgctttat ctgatgtaga caagatctat
    21841 gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta cacgaaccgt
    21901 tcgattactg aaacacttga tctagggtgg gaactgctat cgatgttgcc aagaaccgaa
    21961 ttaaaacgaa tcaaagacga catgctggac aaatatctgc cagaagggaa gtgagttctg
    22021 atggcacgat tgaatgtgaa tccgacccgc atggaattga ctcgattgaa aaaacagttg
    22081 acaactgcca caagagggca taaattatta aaagataagc aagatgaact gatgcgtcaa
    22141 ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga gtctatgaca
    22201 gaagcaatgt ctgattttgt attggcaaat gcttcgatca cggagccttt catcgaagaa
    22261 ttatttgttt tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa tatcatgagt
    22321 gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc tgacagacac acctttggaa
    22381 tacgggtatt tgaattccag tgccgaactg gatcggtcaa tcgatcggtt tactaccctg
    22441 cttccaaaac ttttggaact gacagaaatc gaaaaaactt gtcaactgat ggcgagtgaa
    22501 atcgaaaaga cacgtcggcg agtcaatgca ttagaatata tgacgatccc tcaattagaa
    22561 gaaacgatct attatattcg tatgaagctg gaagaaaacg agcgcgcaga agtaacaagg
    22621 ttgatcaaag taaaaaatat gggtaccact gactaagtgg tatccttttt taaaagagga
    22681 aaacgatgat taaacaaagc aaacaaataa agaaacgctt gccggctgtt cagcttctcg
    22741 cattaggatt ttttctcctg attttagttg gcggcggcct acttacccta ccatttttta
    22801 gtaatagtgg gcatgggaca aattttatcg atgcgctttt cactgcaact tctgctgtct
    22861 gtgtgacggg attgacaaca ttgaatacag cagagcattg gaacagtgct ggtcaatttt
    22921 tgatcatgct tttgattgag atcggcggtc ttggttttat gatggttccg attctatgct
    22981 ttgcattgat aaagaagaaa gtcagtctca gcacacgtat tatattaaaa gaagcgctga
    23041 atctagaaga agtatcaggt gtgatgaagc tcatgcttta catcctgaaa ttggcctttg
    23101 tgatccaact gattggtacg gttgctttat cttttgtatt cgtgcctgaa tttggctggt
    23161 tgaaaggtat ctggtacagt ttgttccatg caatctctag tttttgtaat gcagggtttg
    23221 atttgttagg agacagttta gccaatcacc agcaaaatgt ttatttgatc atggtggtat
    23281 ctgctttgat catttctggt gggttaggct tcatcgtatg gcgtgattta ctggaatatc
    23341 atcagaaaaa gaagataacg attcattcaa aaatcgcatt aaccgttaca ggtacccttt
    23401 tattaggcgg attcgttgtg ttctttttca cagagcgcaa tggggaaacg ctagtggaag
    23461 ggaatatgtt tcaacgattg gcaaacacct tttttatgag tgtgacacct agaacggctg
    23521 gatattattc agttgattac ctccatatga gccatgcagg tctgatttta acgatgtttt
    23581 tgatgtacat cggtggaaca tctgggtcga cggccggcgg attgaaaaca actacgttag
    23641 gtgtgttgct gatccagatg cgttcgatgc ttaaaggacg tacgagagcg gaagcatttg
    23701 gccgtaccat ccgacaaggc gcagtgttgc gggcacttac tttatttttt gttacgttga
    23761 cgttgtgtgt agtcgccatc atgattttat cggtgactga aacgatacct aaaacttctg
    23821 gcatcgaata tattgctttt gaggtcttct ctgcgtttgg tacagtaggt cttacgatgg
    23881 gattgacgcc agacttgact gcaataggga aattgatcat tatagcgctt atgtacattg
    23941 gacgagttgg tgtattgact gtggtcttct ctctgatgac aaaagcacaa agaacacaat
    24001 caaaatacaa atatccagaa gaaagtgtca tgattggata atttcatctt ctgacagaaa
    24061 cctagtgatt tagtaaaata agaaaataaa ataatttttc ttgcttcttg gatactttaa
    24121 gtgcaaaatg ttcgtttatt ttttgaggca ctatccgttg ttgaacctcg ggtagtgcct
    24181 ctttcttatt gaaaggaatc aataagattt gattccttta tttatcaata ggttataatt
    24241 catatttttt tattttttct tttacgatac ttagtccaga agctaaaatt tcaaggttat
    24301 tagaggatag ggaaattcga acgaatgcat ctggttgcgc acgtgtagta aagcgtctgg
    24361 aatgataaag tcggataccg ttagcaagaa agtcattctc aattatctta ggatcaatct
    24421 caggatcaac tggaagaatt cgatagtaag ggacacgatt cgttggtttt tctactttga
    24481 aaagagaatc aaaaagctga ttggctcttt tcgtaagact taatttagtt tctaagatcc
    24541 tatttgcttc atttgaatgg aggatttgag tgatgacttc cacatctaat ccagaagttt
    24601 tcacatttat attaaaaatt gcttgttcta tcttgttttg gacatgtact ggaactacaa
    24661 gataagcaac tcgcaatcca gaacaaataa attttgtcat tccagctaag tagattgttt
    24721 ggttaggtaa taatttttgg aaaggaggaa tgatcgtatg ttgatagtaa gttgttaaaa
    24781 atgaatggat atcatcttca ataacccata gaccttcttt ttcaatcact tcggctaatt
    24841 gctctcgtct tttgtgagag atttgaaacc ctataggatt attacaagaa ggcataagaa
    24901 agacaccatg aatcttcttt tttctacatt cctgaatcaa tatatcaggt cgcattcctt
    24961 gctcgtcaaa atcaattgga acgatctcta aatgatataa ctgagccaat tcaatgaaat
    25021 tagcgtatgt atatcggtct gttgctattc tatcacctgg agagaaaagt gcagaaagtg
    25081 tgatagccaa tccattttga acaccggaaa caatagatag agattctaag gacggaatga
    25141 ctccttgagt ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg cctttgggat
    25201 cttggtaatt aagcaaacca acaagatttg ggttattact agttgattga ataaacggga
    25261 caacgaattt attacattct tcaaaagaac taaccagacc aaaatcaatg atatgttcgg
    25321 ttacttcatt ggtagagatg gtgatggact caaagacatt gggagaaata aatgtgccac
    25381 ttccaatatt cgtatacagc aaacctttct caattccata tttataagcc ttgccaacgg
    25441 tagtaaaatt taaatctaaa taatcagcta gctctctttg ggagggtaac tgagtgtttt
    25501 tctgtaattt attctttaaa atatcttgtt ctaattgttg aatcaacgat cgataataag
    25561 gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg gtaattgtca taagaattaa
    25621 taggcatttt ctacactcct taaaattgta taccatacaa ttttaccatt gattgtattg
    25681 attttagaag ttaatctttt gtgaggaggg aattgtctat gaaaattatt aattttgagg
    25741 aagtagtagc ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa tgtttttttg
    25801 attatgaaag tggtaaaatc agccaaaatc ctcgattggt ggaaatttta cctgacggaa
    25861 aagacaaaaa tatgtttgcg atgatgcctg cttatttggg ggagaacaga tattttggaa
    25921 caaaagtcat taccgctttt ccggataatc atcagtatca tttaccttct catttaggag
    25981 agattttctt gtttgatgct caaaacggac tacccaaagc attgataaac gcaaatgcag
    26041 tcacctggat tcgaacagct gctgtatctg cgctggcaac agattactta gcaaatccgc
    26101 atgcagaaac acttgcttta attggagctg gtcaacaagc attgtcgcat ttgactgcga
    26161 tttctaccat tcgtcccatc actacagtgt ttgtttacga tattttagaa gaaagacgaa
    26221 atacttttat cgctaaagct aaaaaacagt atcctaaaat aacgtttata aatgcaatat
    26281 cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac gttgacctct agtaaagagg
    26341 catttcttca aaaagagtgg gtaagggaag acgcacatat caatgctgtg ggcacattta
    26401 ctcctgatac aagagagata agtacatcgt tgatggaaac ttctcaaatc tatgtagatg
    26461 attactcagc taccttaaca gaaagcgggg acctgcttat agccatggca gaaggagatt
    26521 tagaggaatc ttgtattgtt ggtacattag gcgaattagt ttctgagaaa aaaacatttt
    26581 ataaaggaga atcgaggccg acgattttcg acgcagttgg tctagctatt gaagatcttt
    26641 gttgtgcaga atatatttat aacaaattag agaaaggggc tttaaaatga acttagatta
    26701 ttacgtagtt gatgcatttg cagatgaggt gttcaaagga aatccagcag cggtttatgt
    26761 gctagaagaa tggctgccag aaggtacgat gcaaaaaatt gccattgaaa acaatttatc
    26821 tgaaacagca tttacagtga agaaaaacca agaatttgag cttagatggt ttacgccaga
    26881 tcgagaaatc gatctttgtg gtcacgcgac attagcaaca gcatttgttt tatttaacta
    26941 ttacaaaata cctgatgaaa cgattaaatt ttctactcaa agcggtaatc tatttgtgac
    27001 aagaattaaa gattattatt acatggattt tcctagtatt atgccgaaaa aagtgccgat
    27061 attgactgaa tatgaagaag caattggggc taagattaaa gaagcctact tagcacgtga
    27121 tttatttttc gtattagaag atgaaaaaac ggtggctaaa ttgcagccag attttacagc
    27181 tatcaaaaat tttgaattag gaatagagtc atcgtgacag cagaaagtgt gcaaaaagac
    27241 tttgtttcta gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca
    27301 gcacattcta atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct
    27361 tatcaaatgt catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata
    27421 ataggaggaa aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat
    27481 gaattaaaaa taaattggtg gagagtggca gccttatagc tacaatggtt gttaaaaaaa
    27541 gacgcaaact ttttcaattt aatgaaaagt ttgcgtcttt ttttaattat tggtgtttgt
    27601 tttttattca atcaagagta aaatgcccca tactaactat cctcggatga cttcgatttt
    27661 ataaccatca gggtcaatga caaagtagta agaaggcgta gtgccaggta atccttttaa
    27721 gtcagtaatc tcaagacctg cagctttatg ttgctcgtgt aatttttcta aatcatcagc
    27781 tgcaatagcg atatgaccat agccgtctcc tagatcatac gcaccatgat catagttgta
    27841 agtcaactct aattcatagt catctccagg aagtgtcaaa taaacaagtg taaatttgtt
    27901 ttctggaaaa tcacggcggc ggctttcttc aaaaccaaat gccttttgat aaaattcgat
    27961 tgatgcttca agatctttca cccgaacaca tgtgtgagcc attttcataa attcatctct
    28021 ccttttcctt ttactcaatc ataacaaaaa gaagtaagaa aatagttgat ttgtgcttgt
    28081 tcatgagaaa tttctcatgt aaaatggata gaaaataata ctgttaaggt ggaaacaatt
    28141 catggataaa ccaatttttg gtgaaaaaaa gcaatctgct aattatcaaa cccgttatgc
    28201 agcatatatt gtcatagaaa gagacaagcg gattgcggtg atcgaggctc caaatggtgc
    28261 ttttttcctt cctggtgggg aaatcgaagg agaagagacg aaagaagaag cgattgtccg
    28321 tgagctgata gaagaaatgg gaattgtggc agaaatttct tcttatctgg gagaagctga
    28381 tgagtatttt tactcgaatt atcgtgcaac ttattattat aatccaggat atttctttgt
    28441 tgctgattct tggaagcgta taggagaacc aactgaaaag accaataaaa tttggtgggt
    28501 gagccctcaa gaagcgcttg tgaaattgaa acgtggtagc caccgatggg cagtcgagag
    28561 atggatcgaa aaaaataaaa actgaaagaa agcgagtaga tcagctttct ctcagttttt
    28621 ttatttatta cagtacatat tttttgattg cttcagctac accgtgctcg tcgtttgatg
    28681 aagtgatgac atttgctgct tctttgacaa gagaaacggc atttcccatt gctactccta
    28741 gaccagcata ttcaatcatg gataagtcgt tctcgttatc tccgatagcc atgatttcat
    28801 cttgatcaat accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt
    28861 tatttagtac ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta tcacggaaag
    28921 tttgcggtaa acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc atcatcattt
    28981 tgacgatgtt catgtctggt gtcatttctt caggtgttcg atattttagc ggcatcttga
    29041 ctaaacttgc ttcgtggatc gtataagcgc tgatatctcg attaggcgtg tagatcgctt
    29101 tgtcatcgat cgaatggaga tgactgccga cacggcgtgc catcacttcg atctctagga
    29161 aatcttcatg agtcagtccg tgacgggcga tgattttacc tgatttcgtt tgttgtacga
    29221 gtgccccgtt ataagtaata acgtaatcat tatcttgaaa aaggttcaac tcgtttaact
    29281 gttcctggac acctggtaag ggacggccag tgcataaaac gatcgaaaca ccttttgcaa
    29341 cagcagcggc aatcgcttct tttacttctt ttgttacttc tcttttctca tttaataaag
    29401 taccatcgat gtcgatagcg actagtttga tagacataat ttcctccaat atattttaat
    29461 ttatttcaat caatgcgcca ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa
    29521 ataaatcata atgatcacgc aaggagggat caaccatttc ttttggaaag aaaaatcgct
    29581 catctccttg ttcttgtccc gctaatgcag caacaagctg actaacctgt gataattcta
    29641 tcaaagttcc gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc
    29701 gataaaaatc ataaggcaga tcgtagctag aattcacagc agtgtaataa acagggttat
    29761 agccaacctt ttctactaac aaagtgagtt cttggactaa ttttgaatcc cggttgccag
    29821 aaaaagtagc agatttcagt ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa
    29881 taggatcagc agattcagac cattgagtaa agtaagtgcc caagacccca tcatcaagtt
    29941 tgaggtattc ttctaaagta aagcttccgt caagaaatgg cagaagtaat tgagaatgga
    30001 gttcaaaact gctggcatct tcttgataca actcgtgtgc gcgatgaagc agatgctcca
    30061 aaatcacttc cataccacgg gaaacaggat ggaagtacac ttgcacatac atttgatatc
    30121 ggctaacgat ataatcttcc actgcatgca tgccactcat ggaaaaagcg atcccgcctt
    30181 cataaggacg aatcacacgt aatattcggg taagatcaaa tgttccatat tctgttccag
    30241 tgaaataagc atcccgaagc aaatagtcca ttcgatctgc atcgatctga ctagaaatca
    30301 tttggacaac ttgtgggttg gggtaagttt tttggatcac actagctacc ttttcaggaa
    30361 aaccttcttc tactcggtta agaatctgat acacttctgt ttctggagaa gtgatgatct
    30421 ctacagtgat ggcttcatga ttcgtatgga agatatgttc aaatgtatga gagtaaggac
    30481 catgaccgac atcatgcaac agtgccgcac ataaagtcac cagccgttcc tgatcatccc
    30541 agcctccgtt gcctattttt tcttttgaaa aatttcgaga aaagatatcg cagattcgtc
    30601 ttgaaatctc ataaacgcct aatgaatggg taaaacggct atgttctgct ccgtgaaaag
    30661 tgaaagaaga agtgcctaat tgtttaatgc gtcgtaaacg ttggacttct ttcgaattga
    30721 tcaaatctaa gattacttta tgctggacat ggatatagtt atggactgga tcacgaaaaa
    30781 ctttttccat aggcagcatt tgatatttat aaggggttgt cataatgtag ctcctttatg
    30841 ttattacgtt gttccattct ctggatttgg ttgttccatt gctccagctg gatttctttt
    30901 tttagccagt cggtagaatc ctgtaaatca cttgcagaat acagcttttc aaaagctttt
    30961 gcaaatgctt ctttgacttc ttttacaaaa aggggttggt caagcagttc ttcaagtgtt
    31021 gccattgtag caggatccac tgcaggatag ccatttgtac caaagtcttc ttttaaagca
    31081 gattgataaa atcgcttcat gacttgcccg cgtttttcct gattaccgtt gacacttaag
    31141 tacatcataa cagaaatccc atttttgatc cgacgctgag cgataccggc aaattttttg
    31201 ccatggatgc ttaaatcata agttccagga caataagaat gggtgatttc gaatgcttgt
    31261 atcggtgcct ttggaaaagc gtatttagtc aataggagca tttgctcgta agcgtcatct
    31321 atggttattt tttttgtgtc actttgaggg aaaatcaaag aaatatttaa aattccttca
    31381 tctgatacga tccctaatcc acctgaattt ctgatcactg gagcgtaacc tgcatctttc
    31441 agtgtttgga tcccgtcact tagaaaagga gttcgaacat ccttcatacc taagatgact
    31501 gtgcagtcat attgccagaa atgtaaaaag atttgctggt gttctttaga atattccgtt
    31561 aatatatctg ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg ctgatctagt
    31621 cgaatcgcag ggttcattca accggctcct ttgtataaat tcatattttc tacaatatta
    31681 tactctaaaa tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg
    31741 ttgacaaaac aaaagaaact aggaacaatg tagctatgag aagtaaagga gaatgaataa
    31801 tggaattatc acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag
    31861 gggaagtgca agatttttat tttgatttga aagggcaaat ggtgacaatt ggcgatacgc
    31921 tgtacatacg ttataaagag attcaggagg agacgggcga tgagattcct gtaacgatca
    31981 aattagttcc agatggtcat gtacagctga tccgtgcagg agaaatgcgg atgcgtttga
    32041 aatttggcta taaagaacgg ttagaaacat cctatcgaac accatacggt atgatccaga
    32101 ttgctacttt tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg
    32161 ttcgaatcga ttatgatctg ttcatgggac cggaaaaaat cggcgaatat tatttgacct
    32221 tggattttac agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga
    32281 taaggagtag actgtgaaag gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa
    32341 acaaaagcga attatcaatg atcgaagtag cacacgcgat cttagaacaa cgtggcgatg
    32401 tcatggattt ttctgattta gctaaccaga tccaaaacta tcttgagaaa tcagacagtg
    32461 aaattcgtga ttcactcgca caattttata ctgacttgaa tattgacggc agctttatct
    32521 cattagggga taatcgttgg ggcttgcgtt catggtatgc tatcgattcg atcgatgaag
    32581 aagtaaacca tggaatggac gaagaggatg aagatactcc acgtcgccgt aaacgcaaga
    32641 aagtcaacgc ctttatcaat gatgatgaag acgcgatcga ttacaatgac gatgacccag
    32701 aagatacaga tctgacagaa gaagatgacg atgatctgtt tgatgacgat gatgatgaag
    32761 atgaagaaat cgctgcttat aattctgact tgcaagaaat cggagcagat gacccgacag
    32821 atgatgaaga agacttgcca ggaaatatcg aagaagattt aagtatcata gacgaagacg
    32881 atgatgatga agattatgaa gaggaagaat tttctgactc tgaaaaagaa taaacattat
    32941 tttcttacga gaggttgtga cagagacgat cagcctatca gaataaggag gtgtgacacg
    33001 atttttgtca caccttcttt tttgtatata aaaatcccaa gtaaatacat aaaaaataaa
    33061 aaaattgaaa cttttagcgc accttgtacg tcatattaag tgaggcagaa aggagtggag
    33121 aatgattttt actggatata agaaaaaacg gcagatcgaa aaataccgtc acctgctgaa
    33181 tggactcatc gaaactgatt atcaaaaaat gttccgtatc acgatgaatt acgtacatga
    33241 taaagaagag gccttagatg tgatgcaaga tagttttcat aaagccttga actattttga
    33301 aaaagaaaaa gagatcgagc attttagtgc atggttttat cggatattga tccgcacggc
    33361 attagatagt tggcgcagac aaaaaaggac gcctgtggac ttatttgaaa ttcaagatat
    33421 cccccaacag ttacctttag aaacttccgt tgcagagctc catatgattt tagataaaat
    33481 agacagtccg gaaaaagaga ttctgatcct ctactttttt gaaggatttc gtttgaaaga
    33541 aattgcaact atcttggata tgaatgaaaa cacagttaaa acgaaaatgt accgctcatt
    33601 gaatgcttta cgtcaaatcc tagaatgaag gagagaagag tgccatgtat tcccaaaaag
    33661 aaaatttaaa aaaactaaag aaaacttata aagaacaagc aattcccaaa gggcttaaag
    33721 aggagatccg ccagcgtttt attatcgaag agcaatcatt tataaaagca aggcggagaa
    33781 agagacgtct ccaagggttc ggagtatctt tggctatcgt aatgattaca acaagtagcc
    33841 ttctttttaa tagtcaagtt cgaagctttg cagaagattt acccatctta ggatcagtca
    33901 ttgaattgat cttaggcgaa cgctttactg atcgatctga aaaaatcgat attcaggtac
    33961 cgaagatcaa tacacaaaat gagaaagaaa ataaaacaat tcatggatta aatcaaaaat
    34021 actttcgcga aggacaagca gattttgaaa aagctgaaaa agaatatgga aattttgaaa
    34081 cggatcatta ccaagttttg ggagattatc agaaaatcgt agacgataac cggtttctcg
    34141 tgattgaaag gcagatcacc caaacagcag cagatagcca tgttgaaaaa agatacgaca
    34201 caatcgacaa gaaaaacagt gtccagctct cacttccact tttattcaaa gatgacactt
    34261 atctatctgt tttgacaaaa gaagtcaaac ggcagatggc cgaacaagtc aaggaagatc
    34321 catctaagta ctattggact gagcaagata ttcaagaagg gacgatagaa aaaccaacac
    34381 ttgttacgcc tacacgaagt ttttatttga acaaagagca tcaattagtt ttgacttttt
    34441 ctcaatacga aattgctcct ggttatatgg gtacaccaga atttgtcatt cctaagtccg
    34501 tgacaaaaac gattttggca tcggaagatt atttggatca ttagacgaga agtagttgcg
    34561 actagcagtt tcaattttca agtaagtaca agtaaataat aagaagagaa aaagagaaag
    34621 aagagatata gtgaaaagaa attatttagt catcagtatt attgctttat ccgtgctttt
    34681 gtttttcgaa attacttcgg tggcctcctt gatcaccgaa gtaaaaggac aaacacactc
    34741 ttcggagcat tcgtcacaat ctttcaaaca atcagaacaa tcaaccatta aaagccaatc
    34801 aactgaaaca gctaacgtca aagaagccat tcaagaatca gcggaaaagg tacaaagtca
    34861 atcatcacaa tcaacacaag acagtacaga agcaatccaa gacccgactg tcaaaagcgt
    34921 cgcaatcagc tttgatgatg gaccaggagc gaccacaacg ccgcagcttc tgcgaatctt
    34981 aaaagaaaaa aacgtgcatg tcactttttt cgtcttagga gaaaatacgg cgcagcatcc
    35041 tgaaatcgtg aagcagacag ctgaagcagg gcacgagatt ggcaatcaca catacgatca
    35101 tcagaatctg gccactcttt ctgcccagtc gatgacggag gaagtgacga aagcagacac
    35161 tgagatcaaa aaaagtcact ggcaaaaccc ccgcttttgt tcgtccgccc tatggttctg
    35221 tcacgaatgt aggagcaacg atcatccagc gaccgatcat cgaatggtct gttgattcag
    35281 aagattggaa gacaaggaat cctgatttga ttttgcagaa gatccaagca acagtatacg
    35341 atgggtcaat tattttattc catgatatct atcctgaaac gattcgtgcc gttcctcaag
    35401 tgatcgatta tctgaaagag caaggctatc gcatcacgac agttagcgat ttattagggc
    35461 atccaacggc tgtcgagaac tattatggca gaaacgatca tcggccagtt cagtaagtga
    35521 gacaagtatc tttaataaat aacacatttt aataagtagt tgaacacata actaccttac
    35581 ttgcaaaggc ttaacaata
//
LOCUS       DAZKNO010000006        34024 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000006 DAZKNO010000000
VERSION     DAZKNO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.006"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..959)
                     /locus_tag="ACG8MY_000212"
     CDS             complement(27..959)
                     /locus_tag="ACG8MY_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429447.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(1116..1775)
                     /locus_tag="ACG8MY_000213"
     CDS             complement(1116..1775)
                     /locus_tag="ACG8MY_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0429448.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(1778..2803)
                     /gene="sppA"
                     /locus_tag="ACG8MY_000214"
     CDS             complement(1778..2803)
                     /gene="sppA"
                     /locus_tag="ACG8MY_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0429449.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(2816..3106)
                     /locus_tag="ACG8MY_000215"
     CDS             complement(2816..3106)
                     /locus_tag="ACG8MY_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429450.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            3516..5321
                     /gene="glmS"
                     /locus_tag="ACG8MY_000216"
     CDS             3516..5321
                     /gene="glmS"
                     /locus_tag="ACG8MY_000216"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0429451.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(5393..6748)
                     /gene="glmM"
                     /locus_tag="ACG8MY_000217"
     CDS             complement(5393..6748)
                     /gene="glmM"
                     /locus_tag="ACG8MY_000217"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0429452.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(6772..7941)
                     /locus_tag="ACG8MY_000218"
     CDS             complement(6772..7941)
                     /locus_tag="ACG8MY_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0429453.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(7938..8825)
                     /gene="cdaA"
                     /locus_tag="ACG8MY_000219"
     CDS             complement(7938..8825)
                     /gene="cdaA"
                     /locus_tag="ACG8MY_000219"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0429454.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(9014..12694)
                     /gene="nifJ"
                     /locus_tag="ACG8MY_000220"
     CDS             complement(9014..12694)
                     /gene="nifJ"
                     /locus_tag="ACG8MY_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0429455.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            12892..13413
                     /locus_tag="ACG8MY_000221"
     CDS             12892..13413
                     /locus_tag="ACG8MY_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0429456.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(14112..14677)
                     /locus_tag="ACG8MY_000222"
                     /pseudo
     CDS             complement(14112..14677)
                     /locus_tag="ACG8MY_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14856..15512)
                     /locus_tag="ACG8MY_000223"
     CDS             complement(14856..15512)
                     /locus_tag="ACG8MY_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429457.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(16080..17015)
                     /gene="whiA"
                     /locus_tag="ACG8MY_000224"
     CDS             complement(16080..17015)
                     /gene="whiA"
                     /locus_tag="ACG8MY_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0429458.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(17049..18047)
                     /gene="yvcK"
                     /locus_tag="ACG8MY_000225"
     CDS             complement(17049..18047)
                     /gene="yvcK"
                     /locus_tag="ACG8MY_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0429459.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(18044..18928)
                     /gene="rapZ"
                     /locus_tag="ACG8MY_000226"
     CDS             complement(18044..18928)
                     /gene="rapZ"
                     /locus_tag="ACG8MY_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0429460.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(19063..19794)
                     /locus_tag="ACG8MY_000227"
     CDS             complement(19063..19794)
                     /locus_tag="ACG8MY_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0429461.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(19798..21063)
                     /locus_tag="ACG8MY_000228"
     CDS             complement(19798..21063)
                     /locus_tag="ACG8MY_000228"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0429462.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(21326..24142)
                     /gene="uvrA"
                     /locus_tag="ACG8MY_000229"
     CDS             complement(21326..24142)
                     /gene="uvrA"
                     /locus_tag="ACG8MY_000229"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0429463.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(24152..26146)
                     /gene="uvrB"
                     /locus_tag="ACG8MY_000230"
     CDS             complement(24152..26146)
                     /gene="uvrB"
                     /locus_tag="ACG8MY_000230"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0429464.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            26405..27907
                     /locus_tag="ACG8MY_000231"
     CDS             26405..27907
                     /locus_tag="ACG8MY_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0429465.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            27909..28646
                     /locus_tag="ACG8MY_000232"
     CDS             27909..28646
                     /locus_tag="ACG8MY_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429466.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(29014..30180)
                     /gene="dnaJ"
                     /locus_tag="ACG8MY_000233"
     CDS             complement(29014..30180)
                     /gene="dnaJ"
                     /locus_tag="ACG8MY_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0429467.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(30331..32160)
                     /gene="dnaK"
                     /locus_tag="ACG8MY_000234"
     CDS             complement(30331..32160)
                     /gene="dnaK"
                     /locus_tag="ACG8MY_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0429468.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(32211..32774)
                     /gene="grpE"
                     /locus_tag="ACG8MY_000235"
     CDS             complement(32211..32774)
                     /gene="grpE"
                     /locus_tag="ACG8MY_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0429469.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(32868..33911)
                     /gene="hrcA"
                     /locus_tag="ACG8MY_000236"
     CDS             complement(32868..33911)
                     /gene="hrcA"
                     /locus_tag="ACG8MY_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0429470.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
BASE COUNT         9526 a         7480 c         5948 g        11070 t
ORIGIN      
        1 atttttatct ttttcactga taaaatctaa tggaagaaga tcatttgctg caaaattttt
       61 tctctttttt ttaaaaaatc ttgtttttca tcttctgtac cttcatacat cgtttttcct
      121 gtacacgcct ctacagtcag ttgaaaccag cgcttcgggt cttcgatgta tgtatcatag
      181 acaggataaa caattgcaga ttgcaacgtg aattcttcat ttaacacgga actaagcagc
      241 gcatcttcat taccgtaacg atctgaaaat acacataatt gctggtaact tgcctgctgc
      301 tgcggctttg gcagatcttt tggtatgact ttgactaact tttcttcaat cgtcaaatag
      361 aaaacaggat ggcattcgtt caattgccta agtgtctcca gtaattttgc ccttgctaaa
      421 atatggacct ctggcgatac aataagttta ctcgctgttt ggataaactc atgataagga
      481 agctgttcga tctcatcaat agacgcaagc tgacgtgcag gttccaacga aggaagttcc
      541 tccgatttat tgtggagtgc cttgatcttc tgcctctcca tttgtccgga taattcttct
      601 gcaaattgga ttttttcaat gacccgctgc tcgaaagctt cataaaaatc tgtacttgct
      661 atcaattttc tagcatataa aaaatcttgt gtactgatta acaagtcaaa ataaaattca
      721 gcaaactcct cattttgaag atactgtttt tcatgcaaga gtgcttgctt caatgcttct
      781 tttttctcgt ccaactcgta aagacaaaaa acaagcaggc tattagcctc aaagctttct
      841 ttcaactcgt aagctcgttg aaataattct tttgcctgct gatattgctg gtttgctagt
      901 gcttgttgtg ctactcgcat taaattctca tatttttctg gaaaatcaat cggcttcacg
      961 cagtgtcctc ctcttgctca tgttcagctc atgttcaata gtagcaaaac aaaaaaagca
     1021 tgacaattaa aaataactga agcaccctct aaaagctaga ttttagatct gacttttaga
     1081 aggtgctttc aactgttact gctttttatt gctcatcagc tgttgacttc ctggttgaat
     1141 gctttgatca gattgatcgt aacaacactt gtattagtga aaaaatcgcc tagatgttgc
     1201 tttttctgat taaagattgc tggaagatac cctaacatca gcaaaggaaa ttttagaatg
     1261 aaccggcaag caccttctct aactaaaacg gttgaccagc taagttcttc ttcatcaaag
     1321 ctgatgactc gaataccaaa gatcattttc ccaatcgttt gaccatgatt gaatttcgtc
     1381 aataaaataa aatacgctag ataaataagt aaggctgtga atccgtagat actcagataa
     1441 gaatctgaaa aattaagcca acctagattt ttggcaatac caagtgttaa acgcgtaata
     1501 gctccgatac acaaaagatc gaccaagaaa gcaaacattc ggataaaaaa acctgcatag
     1561 aaatacttag gaaaatcatt tgcttttggc ggttcatttc ttgtgtccgt atactttttc
     1621 catttacgtt gtttcttttt gatttcttcg ggcgtcaaag gctctctttc aaaagagctc
     1681 agtacctttt gggaaaagtt ggagtcaggt cctacagcag gctgctgttg cttatctttt
     1741 tcttgttcct tattttcatt tgaaagctct gacatgctta ctcacctccg taataataca
     1801 ttgcttttgg tgattccgct gtccctagat tctcaagaac ggataagatc tgactagtct
     1861 cagaaggctt caatccctgg aattctgcta gtttacttcc taaccatgta ctagcaaacc
     1921 cagatggcgt ggaagaatat tctactagtt ccgcatcttc aagctgttgt tcctttcgca
     1981 tagcttttaa agcatcttca ggaaatccca attcatcgac taaacctact tcttttgctt
     2041 ggacaccatc ataaatcctt ccatcagcaa tttttttgac ttcttcttca gatttattcc
     2101 gtccctcgct gacgattgct acaaaacgac tatatgcgct gtcgatatat gcttgaagca
     2161 ccgcttcttc ttcttttgtc tctggtcgcg ttcctgaacc catatctttc aacgctccgc
     2221 ttttaactgt tgtatcttcc acacccagtt tttctagaag tcctgaataa ttcaatcctg
     2281 acatgatcac accaattgag ccggtcactg tttcttctgt tgcaaagatt ttatctgctt
     2341 gagcagaaat atagtagcca ccgctagctg ccatgttttt catgctgaca tacattggga
     2401 tatcatgttc tttacgaatc gtatccaatt tcttagcgat ttccgcactt tcgtagacac
     2461 cgccaccagg agaatttact tctaaaaaga tacctttgac tgttggatct tcttcaatcg
     2521 ccttcagttg ttccataaaa tttttatgat tgtaaccttc agaagcaaat aaacctgaag
     2581 accctcccgc agcaatcgtt ccatctactg ataattttac gattttttgc gtactgtctc
     2641 cctcttccaa cacatttgaa gctagttctt catccccata taaccaactg ttcaatccct
     2701 caatttgatc gttttcctga ggcttagtca aactagctga aactaacgaa accacaaata
     2761 atcctgcagc aatcagaact gctgcccaac gtctcttatt cattctttct cctttctaat
     2821 acaagttttt tatgacgatc acagtttctg actgctcaga agtgtcgaac cgctctgctt
     2881 tttcttcccg cgcatacccg gaaaacgact cttttgtcaa ttcatcttct accgtaaact
     2941 cacgagcttc agcaacttcc aaggcatcag gtgcgatttc ttggatgcta tcccagccag
     3001 tatctaaaaa tattttcgta tcatctgata caccagctga tgcttcgaat ttggacaatt
     3061 gtttttttaa cattcccagt gtcatgatct caatttttga atccattttt actcctcctc
     3121 tatccgatta ctttctattg taccgaactc ctgaaaaaga tgatagataa aaacccatta
     3181 ttttttgaca taaaaagagc atttgacaaa gcatttgata tataccaatt tattttttat
     3241 tctttttttg ttgacaagaa cattcgcaaa aggttatgat tttaaatgtt tccagataaa
     3301 aaacaaaaga ggaggatagt tatacctttt aggatagcgc caggtctgtg taaacagatg
     3361 acgaggagag ggtttatcga aagattcggc ggatggccct agggactgca ctctacagaa
     3421 aatccacaaa aaatatctgc aaagataaaa caaaaggatt ttcctgcagc tggaagcatt
     3481 catacgaatt cattatagaa agtaggatgt tttttatgtg cggaattgtt ggaatgattg
     3541 gattaaaaaa tgtaacacca ggattgattg acggtctaga aaaattagaa taccgtggtt
     3601 atgattcagc tggtatcttt gtctcagatg gcacaactga ttatctggtc aaagcacaag
     3661 gcagaattca aaatttgaaa aacaaaataa atattgatac tacaggaaat ataggtattg
     3721 gtcatacacg ctgggcaact cacgggcaac cttctgaaga aaacgcccat ccacatactt
     3781 cccaaagtgg acgcttcgtg ctcgttcaca atggcgtaat cgaaaacttt gaagaactta
     3841 aaattgctta tttagcaaat gaccacttta ttggtgagac cgatactgag attatcgctc
     3901 atctaattga aactttcgcg aaggatacta caacgcaaga ggcgttttta aaagctttgc
     3961 gggtcatcaa aggttcttac gctttcgctt tgattgatcg gacagcacct gatgttattt
     4021 acgtcgctaa aaataaaagt ccactattaa tcggactcgg cgatggattc aatgtgattg
     4081 ccagcgatgc aatggctatg cttgctcata cgaaggaatt cgttgagatt gaagatgaag
     4141 agatggttac cgtcacatca gaaaaagtta ttattcaaaa ttttgcaggt gacatcgtag
     4201 aacgttcttc attcgaagca caagtagatt cttcagatat cgagaaaggt acctatcctt
     4261 tttatatgct gaaagaaatc gacgaacagc ctattatcat gcgtcgtatc gctcaaaaat
     4321 acgtaacaga agacaatcgt gtctctcttg atcaaaagct ggtcgataca ttgtctgata
     4381 gcgatcgaat ctatatcgtt gcttgcggaa ctagttacca tgcggggtta gcaggtaaac
     4441 agactctgga aaaattgaca cagatccctg ttgaggttca tttagccagt gaatttggct
     4501 ataacacacc tcttctttca aaaaaaccat tctttatttt cttgagtcaa agtggcgaaa
     4561 cagcagatag ccgccaagtt ctggtaaaaa tcaaccgtct cggctaccct tcacttacga
     4621 ttacaaatgt tgctggttct actctttcgc gggaagcatc ctttacactg ctattgcatg
     4681 ctggacccga aatcgcagta gcatcaacga aagcttatac tgcacaaata gctgtactaa
     4741 ctttattagc caaagctatc ggtgataaaa aagaatttga aacttctctt gtattcgata
     4801 tcactcatga attgagttta gttgctaatg cgatggaatc ggtcatcgcc caaaaagatt
     4861 atttagaaga gttggcagaa gtatatctca gcgatacaag aaatgccttt tacattggac
     4921 gtggcgctga ctacaacgtt tctcttgaag ctgctttgaa attgaaagaa atcagttata
     4981 tccaggcaga aggttttgcc gctggcgaat tgaaacatgg aacgattgct ttgatcgaag
     5041 aaggaacacc agtgattgga atcatcagtg aagaagtgac tggtgcgcat actcgaggaa
     5101 acctgaaaga agtcgaaagc cgtggcgcta aaacactagt tattgtctca gagggattag
     5161 aaaaggaaag tgaccagctg gtgctaccaa ctgtccaccc ttatctaact actctaacca
     5221 cagtcgtacc gactcaatta cttgcatatt acgcaacgct attacgggga ttcgacgttg
     5281 acaaaccacg taatttagcg aaatcagtga cagtagaata ataaaagaac aaggaccagt
     5341 gtcagacatc tgacactggt ccttgttctt ttatttttta tgaaaatatc tcttaatcca
     5401 gtccgatttc ttctttgact actgccgcga ttttatcgac atagtaatta actttctcat
     5461 cagtaggcgc ttctgccatc actcgaagta atggttctgt accggaagga cgaactagga
     5521 ttcttccttc tgatcccatt tcagcttctg cctcttcaat cacttttttg attgccggaa
     5581 catccatcgc accgttttta tcacttactc ggatattcac taatttttgc ggatagattg
     5641 ttacctctgc tgcaagctct gataatttct tacctgtctg tttcatgata ttcagtaact
     5701 gaattcctga aagcatccca tctccagttg tgttaaaatc taagaaaatc atgtgcccag
     5761 actgctcgcc gccaaagtta taatcgtttt tgcgcatttc ttctacaacg taacgatcac
     5821 caacttgtgt aacgacatct ttcaaaccga ttccttccac cgctttgtgg aatcctaagt
     5881 tactcatgac agtcgtaacg atcgtatctt ttttcaatcg atttttttct gccaagtatt
     5941 tagcacagat aaacatgatt ttatcaccgt cgacaatgtt tccaagttca tccaccgcaa
     6001 taatacgatc gccatcgcca tcgaaagcta gtccagcatc tgctcctttt tcaacgacca
     6061 tctcagctaa acgttctgga tgggtagagc ctacgccatc attgatattt aacccgtctg
     6121 gatttgtgcc catggtataa aaatctgttt caagatcggc aaacaaacga ttaacacttg
     6181 ttgaagttgc tccgtttgct gcatcgacac aaatggtcaa accggaaagg tcaccgttga
     6241 tcgtttgtac aaggaattga gaatatttca ataagccttc tggaaattct tctacagttc
     6301 ccaatccttc agctgatgga cgaggcaatt catccacttc agcatctaat aatgcttcaa
     6361 tttctagctc ttggtcatct actagtttaa aaccatctgc accgaaaaat ttgattccat
     6421 tatcttgtgc tggattatga gaagcagaaa tcatgacccc tgcgcttgct ttttgcaaac
     6481 gtgtcagata agcaacacct ggtgtagaga tcacacctag ctggaacact tcgataccca
     6541 ccgataaaag tcctgaaatc agcgcttctt ctaacatttg acctgagatt cttgtatccc
     6601 gccctactaa cacgcgtggg cgttttcctt cttcttcatg ctggcttagg acataacctc
     6661 cgcaccgtcc cagcttaaat gcaagttctg gcgtaagttc cttgttggct tcaccgcgta
     6721 cgccgtctgt tccaaaatat ttacccatag ttgtaaaatt actcctttaa cttaattttc
     6781 tgaggaattt gttctgctca tttgtgttga ttcgcttgtt tctgatgttt cttctgtact
     6841 tgtggtcgag ctgcttgtcc cagattccga actggaagaa atggttgttg tcgatggttc
     6901 cgttcctgaa tcagcagatg aaatacttct actcgtactt gtattttctt gtgtccgact
     6961 gctcgtcgaa gcagagctac tgcttgacgt ttgcaacacg ggtgtaatct ccacactaac
     7021 tgattcgggc ttgatactta acccctcttc tactggtatt tcaattttac gagttgttcg
     7081 atgattgata cctgagacat tgatcggtac agcaatactg tcaccgatcg atgcaagcaa
     7141 atcgctagag ccgcttactt ctgcttccag ttttgaaaaa ctgaaatcat agctttctac
     7201 cccggcagct ggtgttcctt gctgtatccc ataaagtcgt accgacttgc ttggcttgat
     7261 cgaatcagct ctcactgtta cttgcactgg atcagagacg atacttaata cttcccctgc
     7321 tgcattcagc gcctgtattg ggacctcact attaatccca tcatctgtga ccatcgaagc
     7381 gtccacatca gctactactc gtttgatttc tgccaatgtt ttgtcacctg tagtgataga
     7441 gactttttta ggactaacgg aagtatcatc aagttcatat ccagatggtg tgaacgttga
     7501 aggaatcaac gtttccacat cgaaatcttt tattaccttt ttctcaaccg ttactgtaat
     7561 agtcgatggt tcgatctttg ctgtgacagc actgctcaaa ttttgaattt ttagcggaac
     7621 ctcatgtgtt ccttcgccca gcttagtgat atctgctgtg acgcggaaca tccgtgtatc
     7681 ttcgtttgcc tctgcattca actgaactcg gtttgcactg gaaagtttga cagtcactgc
     7741 attttcatat ccatggatat agtagcgctt tgaatcataa gaaatcttga ttggcacccc
     7801 agtaactgtt tgattatacg cttcatttcc agaaagcgta tttggaacgg actggccatt
     7861 tgcattgaag tacagcgcaa tactgaacag caatgcaata aaactgtaaa gaatgcttgt
     7921 ccgtttctct tttgagatca tttgcgatcc cctcccttag tcataccgtc taggaaagat
     7981 tgaatcacgt ttttcttgtt ttttgttttc tcttcagcaa tcagttctct acgcaaaatt
     8041 gccaaatatt cttgctggga taagccagcc atcagttcat tatctaatgt aatgctgaca
     8101 tccccagttt cttcagagac aacgatggta atcgcgtcgc tgacttcgct tataccgaca
     8161 gctgcccggt gtcgagtccc gaattctttt ggaatcaaca tactttctga tagaggaaga
     8221 taggcgcttg ctactgcgat ctttccttct tttacaataa ctgcaccgtc atgcagcggt
     8281 gtattaggaa taaagatatt gatcagcagc tctccagtga tatctgcatc taatgcaatt
     8341 cctgtctcaa tatactcatc taatccagta tgccgttcga tagtaattag cgcaccgatt
     8401 tttcgcttgg acatatattg gatggctttg tcaaaagaca agatcatctt ctcatcttcc
     8461 tgctgttcgc tttttgattg tttgaaaagc gaactgcgtc caagatgttc cagtcctctc
     8521 cggatttccg gctgaaaaat cacgatagca gctatcaccc cgtacgtaat gacttggttc
     8581 atcagccaag acaaggtgtg cagaccaatg atttccgcta cgattcggat aacaataaaa
     8641 atagctacac ctttcagaag ctgaatcgct tttgtgcctc gcagcagttg gatcagtttg
     8701 tatacaagat accagacaac cagaatatca agtatgttca ccaaaaaatt cagagagaac
     8761 aagtcaccgg aaaacatttg ctgccaatag tccaaattaa aaagttcccc gatttgaaat
     8821 gacataaaaa gcctctcttc tcttttgaga tttcctctct agtatatcat atgttttaat
     8881 tttagaggct gatctgctcg tatttttctt gagaaaaacg cataattttc aggaaaattg
     8941 caagtatgcg ttttattttt ctctgcaata aatattttta aacttcagag aattattcat
     9001 tatatttttt ctgttatttt tccttttttt cttttgtagc ttgttccatt tttttacgtc
     9061 gttctgctct ggcagctctt ctttcttctg cttcaaggtc cgtcgttttt tcttttctag
     9121 tcttaacggg tacatcgata gattcctctg gttcaagtcg tttcttgatt ttttctaatt
     9181 ctcccgtcag tgcagcatag ttgtaaaaac gtgttttagc gtcttggata gtcttttcaa
     9241 ataaagcttt tgcaagattt ggatttgcgt tctctagcga ggaaaagcga acttgtttcc
     9301 tcatgaaatc aggcatagca gagaaatcag gttttttgta gtccaaagtc attgggttct
     9361 tgtttttttc gaccattgca gggttatatc gatataatga ccaatagcca gaagaaacag
     9421 cttcttttgc ttctttaagc gattctttca ttccaccaag caaaccatgt gtgatacaag
     9481 gcgtataggc aatgattaaa gaaggtccag gatacttttc tgcttcttca aaagccttta
     9541 tcgtctgcat ttgattggca cctgaagcaa tttgcgccac ataaacattt ccatatgtca
     9601 tcgccatcat tcctagatct ttctttgaag catactttcc actagccgaa aatttagcga
     9661 ttgccgaagc tggtgttgct tttgaggttt gaccgccagt attggaatag acttcgttat
     9721 ccaaaacaag aatattcacg tctgctccgc tggcaagtac atgatcgatc ccgccaaaac
     9781 caatgtcgta agcccatccg tctccaccaa tcatccattg gctgtttttt acgaatagat
     9841 cctttctctt gtatatatcc tccagttcag ggacaattga tcgttcgctt tcgatcgcag
     9901 cttgcagttt tgctgctctt tgctgcgttc cttcgctctc aaacatgtgt accaaccagt
     9961 cttccatcaa ttctcgtagt tcttctgata cttttggcaa tacttctttg attgaacttg
    10021 ccaaagcctc gcgacgagtc tgtgccgcta gaagcatacc ataaccaaat tcagcattat
    10081 cttccaataa agaatttgac catgcaggcc cttgaccaca ttcattggtc gtataaggag
    10141 aagcaggaga agcaccgccc caaatagaag aacagcctgt cgcattcgcg atcatcattc
    10201 gatcaccgaa catttgtgtc aataatttta cataaggtgt ttcgccacag cctgagcaag
    10261 cgcccgagaa ttcaagaagc ggttgattga attgtgtaga caagacagta ttctttcctt
    10321 tgactggatt tgctttttgt cttaatgtca ttgcaaaagc ccaattcaca gcttgttctt
    10381 tctgttcttc ataaggcttc atcactaaag ctttttcttt tgcgggacaa gcctctacac
    10441 acagaccaca acctgtacag tcttccactg aaacttggat acggtatttt acgccgtctg
    10501 cgcctcgcat ttctctcacg ataaatcctt ctggcgcttc ttccatttcc tcttcatctg
    10561 ctaaaaatgg acggatggca gcatgaggac aaacaaaggc acattcatta cacatcgtac
    10621 agcgatcact aatccactca ggtacttcta aagcgacgcc ccgcttttcc atagaagccg
    10681 ttcctaaagg catttcgcca gttgtcattc catttgctac aagtgtacca acagagagtt
    10741 gatttccttc ctgtgcattc actggttcaa gtatttcttg tatataacca gtgttccttt
    10801 tattgatttt ttcaggcatc tccaacgttc tccaatggtc tgggatcgcg acttgatgaa
    10861 gcagttcggt cgtccgatcg attgcttgta tgtttttttc tacgatagcc attgatttat
    10921 gtccatagct tttcagggct tcttctttta gcagcggaag cacctcatca aaaggaatga
    10981 tgtcagctaa tttaaaaaaa gccgtttcca ttgccgtatt gatccggcgt cccaaaccga
    11041 cttcttgggc caatctcatg gcattgatcg tatagaatct aatgttattt tcagccaagt
    11101 aacgttttaa ttttaagggc agatgcgttt ctaattgttc gtcgctccaa agagtattca
    11161 ataaaaaaat gccacctggc ttcaatcctt tgaccaagtc atagctatgc aggtaagctg
    11221 gtgtgtgaca ggcaacaagg tccgcatgct cgacaagata cgctgaacgt atcggcgtat
    11281 ctccaaaacg aagatgagag acagtcagac cgccagattt tttcgaatca taataaaaat
    11341 agccttgcgc atatttatcg gtgtgatctc caataatctt gatagctgat ttatttgctc
    11401 caactgttcc gtcagatcca aatccccaaa acttggcctg gaatgtttgc ggttctgtca
    11461 aatccagact ttcctttggt tccaaagatt gataagtcac atcatccacg atccctatgg
    11521 taaaccgttt tttgcgtatg cttgggtctt ttttcaattc atcaaaaact gctgagattt
    11581 ggtcgggagt cacatcttta gaccccaatc cgtagcgacc accaataaca gccgggcgaa
    11641 gttcagaatc atataacgca ctttgaacgt ctaataagag aggttctcca cctgcccctg
    11701 gttctttact tcgatcaaga acagcaattg tttttactgt atgaggcaac ttttgcacga
    11761 atgtttccac tggaaaagga cgataaagat ggatattcaa aaaacctgtt ttccgtccgt
    11821 ttgcattcaa gtaatcaatc gtttgttcaa tcgtctgtgc aactgatccc atagaaacaa
    11881 tgacttcttc cgcatcgtct gctccgtaat aagtcaccaa gtcataagaa gttcctcgta
    11941 atcggttgat ctctttcata tatttttgaa cgatttcagg taatttatca taataggggt
    12001 tgatcgtttc ccgttgttga aaatggatgt ctggattctg gttcgtacca ctaacagtcg
    12061 gatgattggg attcatccct cgtttacgaa atgaagctaa tttctcctga ttgatcagag
    12121 gagctaattc ttcatattcc aaaacttcga ttttttgaat ctcgtgactc gtacggaatc
    12181 catcaaagaa attcaaaaaa ggtacttctc cttcaataga agcaagatgg gcaacaggtg
    12241 ctaaatccat cacttcttgt acactgcttt cagctaacat agcaaatcct gtttgtcttg
    12301 cagccatcac gtctccgtga tctccgaaaa tattcaacgc atttgtcgtc actgcccgac
    12361 tagcaacatg gaaaacactt ggcagtagtt ccccagcaat cttgtacata ttcggtatca
    12421 tcaaaagcag cccttgagaa gcggtatatg tagttgtcag tgcaccagtt ttcaatgatc
    12481 catggaccac accagctgct cctgcttcgg attgcatttc tactactttt actggttgac
    12541 cgaatacatt ttcttttcct gctgctgacc attgatctac taattcagcc atggtcgaac
    12601 tgggtgttat tggatatatt gcagcaatct cggtaaatgc ataagaaata tatgcggctg
    12661 ccgtattgcc atccattgtt ttcatttttc gcatgattca tcatccttgt tcatattctc
    12721 tcttattatc tgttccatta taacgtaaaa cattccattt tttcttttat ttataaaaat
    12781 atttcacata tgtctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt
    12841 gccttttttt gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata
    12901 tcagtgattg atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat
    12961 gctcctaaaa aaatggtcga attcatcaac ctatcttgtc cgtattgccg acaatggttt
    13021 gaagaatctt atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc
    13081 aaattgttcg ataaagaaaa agaaagcttg cagcgaggaa acgtgatgca ccggtatctc
    13141 acaatcagcg atggtcaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac
    13201 gagtggaaac atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg
    13261 acagaacaaa aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat
    13321 attcgatttg ttccgactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata
    13381 gaggaattaa aagaattgat ccaaacaaaa taaaaaaata aggagcgtag gacaaaagta
    13441 aagaacactt ttgtctcacg ctctaaacac ctataaacgg cggaagcaga agcaactcct
    13501 tcttatttct tggagttaaa cgcttctgtc ccagcctcct tattccgaat aaatggtgtc
    13561 caaaagcttg catctgccag attatctcat ttcttatgac aagctgagca aattatctcc
    13621 accaaataat ttattcatat ttttgacttg cgcatactaa ctaattctta aattttcata
    13681 caaaatttct caaattccct taacgagtaa tatttatgta cggtgattca cactagtatc
    13741 ccaatcactt tttaagttat cccgatttca attctacttc aaaaatagtt gcttttatgt
    13801 atgctgttag ttgattagat ggtcgtttgt tgagctaggt cgtttatttt aaacaagttg
    13861 tttctttagc ttatacctat ctcgtctacc aaacgcaaga tggcaaacac aaatgcatac
    13921 gcatctgtat ttgccacttt gattcgtata aagctaacaa ctcctaactc ctctcattta
    13981 atagttgctc tcttttttat tcagatacat ttttgctgat cacagcctag tatttcaatg
    14041 gttttgaaca ctaaaccaaa ttgttccatt acaacattca ctggctgatg aaatggatta
    14101 atagatttcg attattcaaa gcgattttct attggtggat tgtgttccca actaacaaga
    14161 cggattccaa agtcattata taactcttca agagttaacc ctttcctgtt cccaatagat
    14221 gtatctctcc cacgctcgat ataaaatatg gagtttcgaa tgccaagacc tggcaaatct
    14281 ttccttgtta cagttacact gctactaact tccccatata aaatccagct cctccaaaat
    14341 gataatatat ttttcgacca tcatctaact catgtaacaa taattgttgt gccctcttgt
    14401 cactcgccaa atgcaaacgc cacgtgcgat accatgccat gctacctgca tctgcagaaa
    14461 atccatcaaa taatgcaaca attgtatctt ctatcacaat cctttcccca tctacttcat
    14521 attctaatct aaaatcaaac tcccctcggg taatcgcagg tcttggtggc ttaggaccca
    14581 atattattct acttaaaacc gcattcgttc ccagatgaaa cacagctata acaacaataa
    14641 tgattaatat tttgtttcta tttgataaat ttctcattat tcgcaaatca cctctacatt
    14701 ctcacatact agttcgtaat actcctttaa tatcaaaata tctactgatc gtaataatat
    14761 tcgctatgaa ttcctactac tcgtataaga aaggccaaat catcagcttt tacttgctga
    14821 tgatttggct tgtttctatt tatcgtaatt gttttctaat caccaaaatt gttaacaatc
    14881 ggttcagata tttcccaatt cactagcctt atattatatt gatttaacaa ttcttcttca
    14941 tttattgtat tttgatcaag cggaccaaat tgttctaata attctttgtc atgtaaaata
    15001 tcccttctag taatgcctct ttccctttca atgaaagaag cactaggaaa agtatgatta
    15061 taaccattca acctatctcc catgtaataa ttagcaggtc ctggaaaata aaatatttgc
    15121 ttattatcac taacttctaa cagcaacact aaatcttctc cgctggctaa acgacttgtc
    15181 catctacgat atctccctat gccttcgttc attccaaatc catcaaattc aactattaac
    15241 gtgtcttcta tcacatgtaa ttcttcattc aattcatact caagtctaaa aggaaattct
    15301 ccatatgtaa tctctggcaa tggtggtgct ggagttctcc aaataaatac aataactgtg
    15361 aatagaaaaa atccaacgat cgatataaat gttatgagac ctgctcgaag taaaacccga
    15421 tataataaaa atgctgtatt tcgctttgct atatctagaa aaataaaaag tactgcaatc
    15481 ataatgatag taaaagttgt aaaaaaacgc atttttgccc tccctttata aacatcgatt
    15541 ctcaaattct aaaccaaatc ttggtagttc aagttcaggt ataacgtatt ccaaccatac
    15601 atctcaatta ctatttatga acttatgatt atcactgaat aaatcactcc atccccttgt
    15661 taaaacttct acattaatta cacctggttg ttttcgacta gctaatccaa tgaacttatc
    15721 aaccatcctt atccaccaac cacgagaatc ttgaccagaa gtaagaagag cagttctcaa
    15781 ggatgatcga gcagttcctt ccagtttttt aattaatcag gccagttata gataaatact
    15841 tgatcttatc tatatcagga cttctatatt aagcaaaaat ttatgtacaa aactaaaatc
    15901 ttcattcagt tgtctccccg ctttttatac gattgtatta aaaacaatag aagcctttcc
    15961 aaattatgtt actccagtaa agatggaatt atagtttgac ggaagaaaag caagtataac
    16021 ttttaaaaaa aactgataca ttcgcatctc ctgcttcatg tatcagtttt ttctttggat
    16081 taaactgctt cttcacgcag tttttccgca aattcattta ttttgcgaat tcggtgatta
    16141 attcccgatt ttgatatcgc acctgaggga atcatttccc ctaattcttt caagctgacc
    16201 tctggatgtt gaagccgaag ttctgcgatt tcctgaagtt tttcaggaag ggcatgtaga
    16261 ccgacctttg cttcgatcaa ttcgatgttt tcgatttgtt tagaagccgc atcaatcgtc
    16321 ttgttcagat ttgctgtctc acaattgact agacgattga ccgaatttct catatcccgt
    16381 acgattcgaa catcttcaaa tcggagcatt gagttggttg caccaatcaa tgttaagaaa
    16441 tcagcaattt tttctgctcc ttttaaataa gaaatatagc cattgcgccg ttcaagcgtt
    16501 cgagcatgta gaccgtaata atttaacata tcgcaaatgt cttggttatg ctgttcataa
    16561 atagaaaata tttccaaatg ataacggctc gtttccggat tattgactga acctgaggcc
    16621 ataaaagcac cacgtaagta agaacgcatt ttttgcgaat ttcccataat ctcgtccgaa
    16681 acgtgtccat tgaacatcat accatccatg atatcaagat ccatcaacac atcttttgtg
    16741 ccttgcttca accggacgat atagacattg ttctttttta atttcatttt tttacgaacg
    16801 agtagctcac ttcgtacctg ataatgatct ttcaaaagag aatagatacg tctggcaatt
    16861 gcagcatttt ctgtttggac gttcaaaata aactgctggt tcacaatacc taaagagccg
    16921 ttcattcgca gcaaagcagc taattccgct ttcgcatgtt ctcgatgaac ttccaaacca
    16981 gttagttctt tcttcacatc tgcagcaaat gacatcctat tctcctcctt tctccaacaa
    17041 taaattgctt aatattttga accgaaaact aaacggaaca attcttctac tactttgtct
    17101 ccatcgtgga agacaccacc atctttcaat tccaaaaagt cggtggaaat cactcgacat
    17161 ccttcatttc tcaatccgtt gaagtcgtgt gcgacttgta ctagatactc atcatagatc
    17221 tctgggtcca tataatcttc aggcactttt tccgtattga ccaatactgt atcgataaaa
    17281 ggctgattca aatgcttgtg taaaacacgt atatgatcgg catctgtgaa atgttccgtt
    17341 tccccttttt gggtcatgat attacagata tagacggttt cagcagttgt cttgagcatt
    17401 gcttcgccga tttctggtat cactaaattt ggaaggatac tggtgaataa acttccgggt
    17461 cccaaaacga ccatgtcggc gtccataatt gctgaaacga cttttcgagc cgctttggct
    17521 tcacttttat catttgtgtt gcagacatac acacggtcaa tggttttacg gtcgatcgcg
    17581 attttagatt ctcctactgc agtcgttccg tctttaaaga cagcatgcaa ggtcaaagga
    17641 gtttctgaag aaggataaac atgtccatct acatgcatca tttttgctaa taattgaatc
    17701 gcctcataag tgcttccccg catctcggaa actgcggcaa tgatcagatt accgatcgta
    17761 tgattggcta aaaactgatc ttcctttttg aaacggtatt ggaaaatatc ttcatagaat
    17821 tgtggcatat ccgataacgc cactaagaca tttctcaagt caccaggagg agccatagcg
    17881 atcgattctc tgattgcacc gctgcttccc ccatcatctg caacagtcac tactgctgtg
    17941 atatctgctc cttgatttcg caaacttttc aggataactg gcagaccagt gccaccgccg
    18001 accactacga tctttggttt tcttatccgg taggttttca ttttcacgac cggttcaccg
    18061 tctctttcct tttatccttg tcccgatgtg tcacattgac tttatagttt ttccctaaag
    18121 catttgccaa acgttcagtc aatgccacag aacggtgttg accgcctgta cagccgattg
    18181 caactgtcaa gctgcttttt ccttctttaa tgtatccagg caatacagtt tccagaagat
    18241 ccaaaaattt atggtagaat gattgcgttt catcaaagga catcacataa tcatagactg
    18301 gctgatcttt tccggtcaat ggtcttagtt ccgggatata atgagggttg ggcaaaaaac
    18361 gcacatccat cacaatatcc gaatcgatcg gcaacccgta tttaaaacca aatgatacaa
    18421 attcgatccg aaatccttgg tctccctttg atttgaatga ctcattgatt ttttcacgta
    18481 attgtcgtgg tgtcaatgtc gtcgtatcaa tgatcacgga agcttgggct ttcaagtcgt
    18541 ctagtatcgc ccgttcttta cgtatccctt cagtgatcaa accgtccatc gccattggat
    18601 gtgctcgtct tgtttctttg tagcgagcca ctagttcttc gtctgaagca tctaaaaata
    18661 agatagaagt gtcgatgaag ttcgtatttt cgatttcgat cagcatgttt tggatttctt
    18721 caaagaagga acgagaacgt aaatccactc ctaaagccat ttttgtgact ttgcctgatt
    18781 ctttgattaa ttcccagaat tttggaatca atcgaggcgg catattatcg atacaaaaat
    18841 agcccatatc ttcaaaactt tggatagcta ctgttttccc tgcaccgctc atacctgtta
    18901 tgatgactaa ttgcaaatta tctgtcataa ttacgaactc ctctcacatt gtgcatagta
    18961 aaattatatc attccgggcg tatcattacc agttttcttt ttcttttaca aaaagatttg
    19021 gaccaaatat cttttaggat atttgcccca aatccaattt ttttactttt ccgatgctgt
    19081 atccaatgcc ttgcttcttt cagctagcgc tcgttcaatc gtccgatctg ctaaaataga
    19141 ttgggcatcg atcactggat aatggttatc ttcagcaaat tcatgcatca atgacaattc
    19201 tgtgcaacct aagataactt tttcacagtt taggcgctct accgcttctt ctaagatttc
    19261 gtaatatagc tcttgattca aataatccga ttccttgatc tcatgataga tcagataatt
    19321 gattttttct tgaagaacag tatcaggaat cactgtttcg aatccaagat ttttgacttc
    19381 gcgttcatag attccagctt tcatacttcc ttctgttcct agaatcgcta ctctccctgt
    19441 cgtatgctga cggacaagtt catttgctgc ttctcttggc atatgaagga taggaatatc
    19501 cgttgccgct tgtaattctt caaaaaagta gtgtgcggtg ttacaagtca acacaatgaa
    19561 gttcggctgc aataaatttt gtttctcaat atcatccagc agaaacggca ttggattttc
    19621 ttctgatcga tctaaaatat aagctgtacg atcaggaact gttgcatgat tgaataagac
    19681 ataattcaaa tattcttgat cttttgtagc tttcgttcgg tggttgatca agcgaacaaa
    19741 actttctgtc gccatcgtgc ccattccgcc taaaatactg aaaaaattct ccatgaacta
    19801 gccttctttc acatgaaaat actttttgaa tcttggaata tagttatgga acatatattt
    19861 catcaacaac cagcgcttga ttgaccggtc tttttcataa aacacagtag taccgtaacg
    19921 attttctttg atcatttgct cagctagttt tttgtcttca ttttcccgag catattctaa
    19981 aaagattttt ttgggcacac ccatccataa tcttgcttta tctgattgat tcgttccata
    20041 ggtcgtttca acaaagggtt tgttaaacac tcgatcttct gtgacgaaac gagccagatt
    20101 caagccgttc aatgtcacaa agaaactgct acgaccttgt ctcaaattga tttcaaataa
    20161 tttatattct ccatctctag gatcatactt catatcgaaa ttggcaaagc ctgtatattc
    20221 gattttttcc aaaaatgcct taatggtttg atagattttt tcgttataat ctggcatgat
    20281 cacaacgtaa ttaccaatcg atgctggtgt aggatcttct aacagtggat gaccaagaca
    20341 catcatccga acttgatgat cttcatctac atacgcattt agcacacgca tattactatc
    20401 atcacccggg ataaaatctt gtacaatcat ttcactttta taaccggctt cgtatatccg
    20461 gcctaaaatt aaatcgaact cttctctagt ctctaaaata aaagcttttt ttcttccttc
    20521 aaattggaca gaaagatact ccacactgtt tgcaggtttc aaagcaactg ggaattcaaa
    20581 aggcaattcc tgttccaaat gtccatttac taacatttct tctctgacga tcaatgtttt
    20641 aggatacgga agatcatatt cttcacaaac ttcataaaag cttactttat tgatcagtct
    20701 ttcaaataaa gaataatcaa tatatggaca gataaaatat tctgacagct cttgtttatg
    20761 ttgggagatc aattctgcat atccatcacc acaagcaatc aaaagatatt ttttactctt
    20821 atcagaatat ctttcttttg ccaaacgaag catcgtctcg ataaacacag ggtctttatc
    20881 aaatcctgga ataacttcta cattgacaat tttactgtag cgggtagggg ctaactgatc
    20941 cgaagcatat gcttggcaga ttttgccgta tgcttcatta aaagaacgtg ccataccata
    21001 aacgttcatg tcgcttccta ataatatagg gataaattct tcattttcaa tactgttcat
    21061 cattatctct cacttactca taatctcttg tttttctttt ccaactacac aaggaatatc
    21121 ataccataaa gaaaaaagca atcaaaggaa actcctccga ttgctcaatt cttttttgaa
    21181 cggattgaac ttataggaag tttttgtcta taccaatcag tgtagaaaga actaccatgt
    21241 aatgattcta gaacactgat attaataaaa caagcaaaaa ttcatttttt tatgtttttt
    21301 taactatctc agctatctat cctctttata ccattatttt tttcaaatag tgacctgtat
    21361 aactttcttt tacgttaatg atttcttctg gtgttccagt cgcaaggatc gttcctccac
    21421 cttctccacc ttctggtcct aaatcaatga tataatctgc tgttttgatt acatctagat
    21481 tgtgttcaat cactaaaact gtgttaccag catctactaa tctttgtaat acatgcaaca
    21541 agcgggcgat gtcatcgcta tgaagtcctg tcgttggttc atctagtata tagaaattct
    21601 ttccattaga gattttgtgc aattcactgg caagtttcat ccgttgtgcc tcaccaccgg
    21661 acaatgtcgt tgctggttgc cccatcgtca cataacctaa gccaacatca acaatcgttt
    21721 gcagtttgcg atgaatcttt ggaatgtgct tgaagaattc tacagcatct tctactgtca
    21781 tttccaaaat atcagcaatg ctttttcctt tgtaatgcac ttctaatgtt tcagagttat
    21841 atcgtttgcc atgacatact tcacaaggaa catagacatc aggcaaaaag tgcatttcga
    21901 tcttaataat tccatccccg cgacaagctt cacaacgacc gccttttacg ttgaaactaa
    21961 aacgcccttt cttataaccc cgcatttttg cctcgttcgt ttgagcaaat aacccacgga
    22021 tatcatcaaa tacacttgta taagtcgctg gattacttct cggcgtccgg ccgattgggc
    22081 tttgatcgat atcgatgatc ttttcgatac tttcgtagcc ggaaattgtc ttgaatttac
    22141 ctggcttagc agaattctta tttaattttt gcgctaacga tttctttaag atactattga
    22201 ccaatgtact ctttccagaa ccagacacac ctgtgactgc tacaaattct cctaatggaa
    22261 attcaacact aatatttttc agattgtttt ctgaagcacc agtgatcttg attgctttcc
    22321 cattaccttt acggcgttct tttggtacag ggattgattt tttccctgaa agatatttcc
    22381 ctgtcaaaga attttcatca ttagctactt cttcaggagt gcctgcagcc acgatttgtc
    22441 cgccttgttc tccggcccca ggtccgacgt caatcagata atctgctgcc cacatcgtat
    22501 cttcatcatg ttcgactaca acgagggtgt tgccaagatc acgcattttc tttaatgagc
    22561 caatcagtcg atcgttgtcg cgttgatgca atccgatcga tggttcatct aaaatataca
    22621 aaactccaga aagattcgat ccaatttgtg tagccaatct gattcgctgc gcttctcccc
    22681 cagataatgt tccggaagcg cgactcaaag ttagatagtc tagtcccaca ttttccaaga
    22741 atgaaagtct gtcatttact tcttttaaaa tcggtttggc aattactttc tcttgttcag
    22801 ataacgttaa gtttgagaag aaatctactg ctttttgaat agacaactca tttgtttgcc
    22861 cgatattcgt tccattgatt tgaacagata gagcttgggg attcaatcga taacctttac
    22921 atgtctgaca agttaattct gtcatataaa gacgcatttg ctcacgcgtg aaatcactgt
    22981 tcgtatcatg ataacgacgc tcgatattct tcaatacccc ttcgaaagtc gtttctacat
    23041 cgcgaacgcc accaaaatca ttttcataat ggaaatggaa aagatcatct gtcccattca
    23101 agatgatatc ctgatgctct tccggtaatt cttcgaatgg tgtatctaaa tcgataccga
    23161 aactttcaca agcctgcgct aacatttgag gataatactg agagctgatt ggattccatg
    23221 gaacgatagc accttgacta agtgtcttcg ttctatctgg aatcactaaa tcttgatcga
    23281 cttctaattt gacacctaaa ccatcacagt ctggacaagc tccaaaaggc gcattgaatg
    23341 agaacagtct tggttctagt tcgccaacag taaatccgca ataaggacag gcatagtgtt
    23401 cgctaaacaa catttcttct tcaccgatca cgtcaaccag agcatagccg tctgccaatc
    23461 gcagagccgc ctctaaagaa tcaaatagac gagaacgaat cccttctttc acaatgatcc
    23521 ggtcgatgac gatcgcgata tcatgttttt tgtttttctc cagctcaggc acttcactta
    23581 aatcatagat ctcaccatcg actcgaacgc ggacgtatcc ctctttttgg attcgttcga
    23641 atattttttt atgttgtcct tttttcttta ccacgatcgg tgccaacact tggatctttg
    23701 tgcgctcagg caaagccatt acttgatctg ccatttgatc tactgattga ctggtaatct
    23761 cgatatgatc gttgggacaa atcggatgtc ctacacgtgc aaacaacaag cgtaaatagt
    23821 cattgatttc cgtcacagta cctactgtcg accgaggatt tttacttgtt gttttctgat
    23881 caatagaaat cgccggactc aatccgtcga tgctatctac gtccggctta tccatttgtc
    23941 ctaaaaactg acgagcataa gcagaaaggc tctctacgta tctccgctgc ccttcagcgt
    24001 acagcgtatc aaatgctagg gaactttttc cagaaccgga caaacctgta acgaccacga
    24061 atttatctcg tggaatcgtt acatctatat ttttcaagtt atgggcacgt gcaccgtgaa
    24121 tcgtaatttt atcgttagcc atgttgtcct cctactttga agcttttaat tctaaaatca
    24181 tatctcgcac attagcggct gtttcaaaat ccaatgcttt cgccgcattt ttcatttctt
    24241 tttctaattt catcaataac gtatcttttt cttctttcgt catttcttca tacgagactt
    24301 gtaccgcttc ttttgtatca tcttccgatt ctttcgtaat agaaatcaaa tctcggattt
    24361 ctttaatgat cgtttttgga acgatcccat gttcttcatt gtacttctgc tgaatcgtac
    24421 ggcgtcttga tgtttcatcc atcgctagac gcatagaatc tgttactttg tctgcgtaca
    24481 tgattacttt accttcttca ttccgagcag ctcgtccaat cgtttggact aaggaacgtt
    24541 cactacgtaa aaagccttct ttatccgcat ccaagatagc aacaagggaa acttcaggta
    24601 cgtctaatcc ttcacgaagc aaattgattc caactaacac atcaaattca cctaatcgaa
    24661 gatctcgaat gatttctgtt cgttccagcg ttttgatatc actatgcaaa tatttgactt
    24721 tgatccctaa ttctttgaaa taatctgtga ggtcttctgc catttttttc gtcaatgtcg
    24781 ttacaaacac ccgctggtct ttttcgactc gttcatttat ttcgcctact agatcatcga
    24841 tttgtcccat gattggacga acttcgatca ctgggtcaag cagccctgtc ggacggatga
    24901 tttgttggat aacggtatct gtttgttcat gttcatatgg tcctggcgtc gcagagacat
    24961 aaatgatctg attgacatgt ttttcaaact cttctaaccg aagtggccga ttgtctaaag
    25021 cagatggcaa acggaaaccg taatctacta gcatctgttt tctcgctcgg tcaccattat
    25081 acattcctct gacttgaggc atggttacgt gcgattcgtc cgccacgatc aaaaaatctt
    25141 caggaaagaa atccaatagc gtataaggcg gttcaccttc tttacgtccg tccatatgcc
    25201 gagaataatt ttcaattccc gacgtgtagc ccatttctaa catcatttca atatcgtaat
    25261 tggttcgctg ttctaaacgt tgagcttcca gaagtttgtt ttcatttcgc aagacagtta
    25321 aacgctgttc taattccgct ttgatattcg ctacggcatg ctccatatgt tcatcattcg
    25381 tcacaaagtg agtcgccggg aagattgcca cgtgttccgt ttctcctaaa acttcacctg
    25441 tcagtgcatt gacttctcgg atacgttcga cctcatctcc aaaaaactcc acacgtaagg
    25501 cacgttcatc tcttgaagcc ggaaaaatct ccacgacatc accgcgcact cgaaaacgac
    25561 cgcgctgaaa atctatatca tttctttcga attggatact gactaagtca cgaatcaact
    25621 gattgcgatc tagttctgct ccctgtcgaa tcgaaactac ttgtttttgg tactcaaaag
    25681 gcgaacctaa tccaaaaata catgaaacgg atgcaatgac gatcacatca tttctttcta
    25741 ataatgaact agtagccgaa tgtcgtaatt tgtcgatctc atcatttaca cttgaatctt
    25801 tttcgatata cgtatcgctt gaaggcacat aagcttcagg ctgataataa tcataataag
    25861 aaacaaaata ttcaacagca ttgttaggga aaaattcctt gaattccccg tacagctgac
    25921 cagccaatgt tttgttatgg gcaataatta gtgttggttt attcactttc tcaataagat
    25981 tggaaatcgt atatgttttc cctgttccag tcgcacctaa taagatttgt gcttttttcc
    26041 cgccgactac gccatccact aattggttaa tcgcttcagg ctgatcacca gccggttggt
    26101 atttcgagac aagctcaaag tgtcgggagg tatctctctc aatcattctt cgtcctcctt
    26161 ttcattcatt ccttttcatt ttacgaccaa taaaaagaaa aggaaagcaa aggcattcct
    26221 tactaaaaaa ttataccaca ccgaacatac tttcgccact tttttatctt attatgcatg
    26281 ttacgtttta taacataaaa tgttagaaac tcgcattttt ttcatttttt cgagcttaca
    26341 gtcttgtctg attcagcact tttcgcctat aataataaaa tattggaaat ttaggaggtt
    26401 gattatgaaa agaaagcatt tatttcactc atttgttttc ttaatgacag gacttgtgac
    26461 acttgcgttt ggacatgttg cacaagctga caacactaca acggcagagt caagcacaag
    26521 cgagacaatg caaaaaatag agcccaaaaa agacgtatac gtaattgcca gtgactctgc
    26581 ttttgctcca tttgaatttc aaaacgcaaa gggcgagtac gaaggcatcg atgtaaaatt
    26641 gatggaaaac atcgccaaac tgcaaggatt taacatccga atggactacc gtggtttctc
    26701 tgctgctttg caagctttgg aatcaggaca agcagacgga atgattgccg gaatgactgt
    26761 aacagatgaa cgaaagaaaa gctatgattt ttctgattct tactttgata gcgggatcca
    26821 attagctgtc aaaaaaggag atacttcgat caagtcttat gaagatttga aaggaaaaac
    26881 agtcggagca aaagttggta ccgaaagtgc tgaattttta agcgaccatg aatctgaata
    26941 tggctattct atcaaatatt ttgatgctgc tgatcaattg tacgacgggt taaaagttgg
    27001 cgcaatcgat gcaatgatgg atgattatcc ggtaatcggt tatgcgatca aacaaggaca
    27061 agaattggaa acaccgatca aaaaagaatc tggcggaaaa tatgcatttg ccgttaaaaa
    27121 aggacaaaat cctgagcttc ttaatatgtt caatgaagga ttgaaagaat taaaaagaac
    27181 aggcgaatac gataagatta ttaaagaata tgtagcggat ggttcagaaa cttccagtga
    27241 gtcttccact gctgatgaat caacatttct tggacttatc caaaacaact atcaagcatt
    27301 gttgaaagga ctttggagaa caattctcct aactttgatc tcctttgctt tggctatagt
    27361 aatcggtgtg atttttggat tattcagtgt agcaccaata cgcgctcttc gcacaattgc
    27421 tagtatttat gtcgatatca tccgaggaat tccaatgatg gttcttgcgt tctttatttt
    27481 ctttgggttg cctggtatcg ttggatttac gattcctgac ttccttgctg gtgttatcac
    27541 cttgactctt aacgcaagtg cttatatcgc agagattgtt cgaggcggga tcaacgccgt
    27601 tcctgtcgga caaatggaag cttccagaag tttaggtctt tcttataatc ggacaatgca
    27661 aaaaatcatt ttgccgcaag cgatacgcat catgatccca tcattcgtca atcagttcgt
    27721 tatctcatta aaagacacaa cgatcatttc cgcaatcggt gtagtcgaat tgctgcaaac
    27781 tggtaagatc attgttgcac gtacaacaca aagtacgtat gtttacttga tcattgcgat
    27841 catgtacttg attttgatca cagcattgac aaaattagca aaagttttag aaaagaaggt
    27901 gaagtaagat ggcagaaaaa atcttagttg agcatttagt caaaaaatac ggcgataaca
    27961 cagtattaaa cgacatcaat gtgtccatca acgaaggaga tgtcgtctgt atcattggcc
    28021 cttcaggttc aggaaaaagt acatttttgc gttgcttgaa tcgtttagaa gaaccttctg
    28081 acggtgaaat tattattgac ggcgcccact tgatggataa aagcacagat atcaacttgg
    28141 tgagacaaca tatcggaatg gtattccagc actttaacct attccctcat ctttctattt
    28201 tggaaaacat cattttagca ccgatggacc tgaagaaaga atctagagaa gaagcagaga
    28261 aaaaagcaat caaattgctg cagacagttg gtctagaaga taaaaaagat atgtatcctg
    28321 aaatgctttc cggtggacaa aagcagcgtg tggctattgc tcgtgctcta gcgatgaacc
    28381 ctgacatcat gctttttgat gagcctacat ctgcactcga ccccgaaatg gtcggtgatg
    28441 tcttgaacgt catgaaaaag ttagcagaac aaggtatgac gatggttatc gttacgcacg
    28501 aaatgggctt cgctaaagaa gtggccaatc gtgtcatgtt cattgacgac ggaaactttt
    28561 tagaagacgg aacgcctcaa caaatctttg aaaatccgca aaatgaacgg acaaaagatt
    28621 tcttgaataa agtattaaat atttaataaa aaccaaaaat gcccggtcgc ttcatgacga
    28681 ccgggcattt ttagtttctt gttttttcac ttaactgtac cagccgcatc gcttgttctc
    28741 ctgcacaaac aagcaatcgc tctacctgtt ccatcgcata agaaagtacc atcggttcat
    28801 tgacgatatc aagtacatag tcgataccac cttcagaaac tatgatgatt atcttattct
    28861 aaagctggct tttttgttgg tacaaacatg acaactacaa aaaaaccagg cgaacgtatg
    28921 attgttcgtc tggtttttaa ttattaatga cttagcgttt ttactagaaa tagaaaattt
    28981 gggcaagtct actttatatt tttactagaa aaactacttc cgctttttcc cgccaaaagc
    29041 atctttaaac ttatcgaaga aaccgtcttc ttgctgttct tcaactttga tgccgccagc
    29101 ttcagcgaat gcacgtaatg cttctctttg ttgttcattt agatttttcg gtgtgatcag
    29161 ttttactttg acatgttggt cgcctgttgc accaccacgt aacttcggag cgccttttcc
    29221 acgtaaacgg aagttcgtac ctgtctgtgt tccagcaggg atcttcaatt tcacatctcc
    29281 atggacagtt ggtacttgga cttcatcccc caaagctgct tgtacaaaac ttaaaggcaa
    29341 ttcatagtaa atttcagaac cttcacgatc aaagatatca ctttcttcta cacgaaaaac
    29401 cacatacaaa tcaccaaatg gtccgccgtt ttctccagct tctccttgac cagctaaacg
    29461 catttgttgt ccttcttcta caccagctgg tacattcact ttaactgaat gtgctttctt
    29521 ctcatgtcct gagccatgac aggttggaca aggttctttg atttctttcc ctgtgccatg
    29581 acaaacatca caagtttgac ggctcatcac tcgtccaagt ggcgtttggc gttctacatt
    29641 gatcgtacct gcaccatgac atttatgaca agtagttggt tgtgtgcctg gttttgcacc
    29701 gtttccacca caagtatgac agatttcttc ccgattatac ttgatttctt tttctacacc
    29761 aaaaatcgct tcttcaaaag acaagtcgac cgtatattgc aaatcagcac cttgacgagg
    29821 cgcatttgga tcgactgacc gaccgcctcc tccaaagaaa gagtcgaaga tatcttcaaa
    29881 accaccgaag ccgccaccag agaagccgcc gaatccacca aatccgccac cagctccacc
    29941 agcaccgtaa tttggatctg tgcctgcatg cccgtattgg tcgtatgcag cgcgtttttg
    30001 cgggtcactt aaaatctcat atgcttctga tacttctttg aatttttctt ccgcatcagg
    30061 ttctttattg atgtcaggat ggtatttttt cgaaagctta cgataagctt tctttatttc
    30121 atcttctgat gctccttttg agagccctaa gacttcataa taatcacgtt ttgttgccat
    30181 aggctttcct cctattggtt ctcatcattt tcacgtcgct tgttatcata tcatattttg
    30241 tttgaaacaa aaaccgatac ttcaaactat ttttcaaaag aaaaagccaa agtagcaact
    30301 ttggccttct ctcgaaattc agtgaactga ttatttatcg tcgccatcga cttcttcaaa
    30361 atcagcatca acgacatcat ctgctccgcc ttgagctgct tctggatttt cttgagcttg
    30421 ttgttgtgca gcttgttcgt atagtttaac agtcaagttt tgaacgattt cgttcaatgc
    30481 atcgcgttta gttttcattt cctcgatgtt gtttgcttca acagcggctt ttaattcgtc
    30541 acgagctgtt tctgcttttt tcacttcttc ttcgtccact ttgccttcta attctttcaa
    30601 tgttttgtcc acagagaata gcaatgcgtc tacatcatta cgtaggtcaa cttcctcttt
    30661 acgagcttta tctgcttcag cgtttgcttc tgcatctttc accatacgtt cgatttcttc
    30721 gtctgttaga cctgaagaag atttgatcgt gattttttgt tctttttgtg tacctagatc
    30781 tttcgcagat acatttacaa tcccgttttt atcaatatcg aatgttactt cgatttgtgg
    30841 aataccacga ggagctgctg gaatatctgt caattggaat cttcctagag ttttgttgtc
    30901 agcagccatt gggcgttcac cttgtaatac atggatgtct actgctggtt gattatctgc
    30961 cgcagttgag aatacttgtg atttacttgt tggaatcgtt gtattgcggt caatcaattt
    31021 agtgaacact ccacccattg tttcgatacc taatgacaat ggtgtaacgt caagtaaaac
    31081 aacatctttc acatcaccag tgatcacgcc accttggata gcagcaccca tagcaactac
    31141 ttcatcaggg ttaactgatt tgtttggttc ttttcctgtt tctttacgta ctgcttctac
    31201 aactgcaggg atacgagtag atccaccgac taaaataact tcgtcgattt ctgattgtga
    31261 cagacctgca tcttttaacg cttgacgtac tggtactttt gtacgttcta ctaagtcagc
    31321 tgttaattca tcaaattttg cacgtgtcaa tgtcatttcc aaatgcaatg gtccagattc
    31381 tcctgctgtg ataaatggca agctgatttg tgtgcttgat acgccagata aatctttttt
    31441 cgctttttca gcagcgtctt tcaagcgttg taaagccatt ttgtcttgag acaagtcaat
    31501 gctgttttct tttttgaatt ctgctaccat gtagtcgatg attttgttat caaagtcgtc
    31561 cccacctaga tggttgtcgc cagctgttga caatacatca aagacaccgt ctcctagttc
    31621 aaggatagat acgtcaaaag taccgccacc aaggtcgaat actaagattt tttcgtcgcg
    31681 atctgtttta tctaaaccat aggcaagagc agctgcagtt ggttcattga cgatacgttc
    31741 tacttctaga ccagcgatct taccagcatc ttttgttgct tgacgttgtg catcattaaa
    31801 gtaagctggc acagtgataa ctgctttttc gactttttca cctaaatagt cttctgcgaa
    31861 tcctttgata tattgaagga tcattgctga gatttcttgt ggtgtatatg atttaccttc
    31921 tacatctact ttgtaacctg cttcaccgat atggcgtttg attgatgaaa tcgtatttgg
    31981 gtttgtcaca gcttgacgtt ttgcaacttc ccctacttgg atttctccat ttttgaatga
    32041 tacaacagaa ggtgttgtac ggtttccttc tgggttagcg atgatttttg cttcgtttcc
    32101 ttccaatact gctactgcag agttagtcgt acctaagtca ataccgataa ttttactcat
    32161 aattaacgtc tccttcttta attgaaagtg taagttttat atccgcacaa ttattgtgca
    32221 acaatgacca tagttggtct taacacgcga tcatgtaatt tgtagccttc ttgtagtact
    32281 tctacaatcg tatcggctgg tgtatcttca gtcgctggaa cagtttgtac tgcttgatga
    32341 agattaggat caaaggcttc tcctttagca gggatttttt caattccttc ttcttccagt
    32401 gcgttacgca aactttccaa gaccatttcc acgccttttt tcaaacctgc cccttgatca
    32461 tctgacactt ctgtggctaa tgcacgttcc aggttgtcaa ttgatggaag taattttttg
    32521 gctaaatctt gtgaacggta tttttgtaat tgctcacgct cattttttcc tcgatttgcc
    32581 atatttgcga tttcagcacg agcccgcaag tatttatctt ccatttcttc taactcagct
    32641 tttaatttcg ctgtttcgaa ttcttccgct tcagcttctg aaatacccga atcttctgca
    32701 gcttcgacat cgatttccgg ttctggctga gcatctgtca tttctttatc tagttcttct
    32761 tgattttctt tcacgataca gtcttccttt cattattcag tcagctgttt gattgctctt
    32821 actaagaaag agagaacgat cagctgcaaa tagcttcgtt attcacatta cgagtttgac
    32881 aagtcaagcg agcggtagta atcagcaagt ttagctgcta gttctcttcg ataaacatcg
    32941 actagaccga acatccgaga ataaggcata ctagtaggcc ctaacaaggc tattgtacct
    33001 cttccatgac catctatctc gtaacttgct tgaatcatac tcatattctg caataaatca
    33061 ttgcctaatt cagcaccaat cttgatatga atattgctat ccatcggagt gatcatttgt
    33121 gtaagtccgt ctgagtcttt cataaaagaa aacatcgatt tgaactgatc aagatcttga
    33181 cggggctcat aatccaagag attcatttgt ccactgacat aaaccttttc ttcaaaaaca
    33241 tgaccaagaa cggtgtcaaa taactgagac atcccttctg tcgtctggaa atatttatga
    33301 agaatcatcg gaatctccgt acgaagttta tggtacaccg tcatcagagg atctccgact
    33361 agacggtcgt ttacaatctg taccattttt tctagttcct gacttcccat atattcggga
    33421 agagtgaaca cttggctttc tacattcccc atatctgtta cgacgatagc aataatctgt
    33481 cgattattca agggtacgat acgaaagcca gtcagtcggc ggtcttttat ttcaggtccc
    33541 aatgaaaaag tcgtatagct tgtcagctca gataatatct cagcagattg cttaataatc
    33601 tcattgattt catgaaattc tctgcctaga gactgacgaa tcagcagcaa atcatcttct
    33661 gctacgcgag caggttttaa caaatgatcc acgtaataac gatagcctaa agcagatgga
    33721 atccgcccag aagaagagtg agtcttcaac agcaatcctt catcttctaa tgctttcata
    33781 tcattgcgaa tcgttgctga gctggccttg actccctcag acattaatgt tttggaacca
    33841 acgggcacgc cactactcgt atagtgttgg atgatcagac gtaaaatgtc gttttgtcgc
    33901 tttgttaaca tcttcctcac ctctcattag cactcatata ttctaagtgc taattcactt
    33961 ataaatatag caagaagccc ttctcctgtc aagagaaaga ctccattttt tagcactcta
    34021 aaca
//
LOCUS       DAZKNO010000007        33355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000007 DAZKNO010000000
VERSION     DAZKNO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.007"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..683)
                     /locus_tag="ACG8MY_000237"
     CDS             complement(105..683)
                     /locus_tag="ACG8MY_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0429471.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(703..1371)
                     /locus_tag="ACG8MY_000238"
     CDS             complement(703..1371)
                     /locus_tag="ACG8MY_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429472.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1386..2456)
                     /locus_tag="ACG8MY_000239"
     CDS             complement(1386..2456)
                     /locus_tag="ACG8MY_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429473.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(2627..3991)
                     /locus_tag="ACG8MY_000240"
     CDS             complement(2627..3991)
                     /locus_tag="ACG8MY_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0429474.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(3988..5547)
                     /locus_tag="ACG8MY_000241"
     CDS             complement(3988..5547)
                     /locus_tag="ACG8MY_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0429475.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(5603..8083)
                     /locus_tag="ACG8MY_000242"
     CDS             complement(5603..8083)
                     /locus_tag="ACG8MY_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429476.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(8067..8402)
                     /locus_tag="ACG8MY_000243"
     CDS             complement(8067..8402)
                     /locus_tag="ACG8MY_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429477.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(8416..9450)
                     /locus_tag="ACG8MY_000244"
     CDS             complement(8416..9450)
                     /locus_tag="ACG8MY_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0429478.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(9540..10220)
                     /locus_tag="ACG8MY_000245"
     CDS             complement(9540..10220)
                     /locus_tag="ACG8MY_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0429479.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(10504..11607)
                     /locus_tag="ACG8MY_000246"
     CDS             complement(10504..11607)
                     /locus_tag="ACG8MY_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0429480.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(12236..12706)
                     /locus_tag="ACG8MY_000247"
     CDS             complement(12236..12706)
                     /locus_tag="ACG8MY_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0429481.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            13036..14676
                     /locus_tag="ACG8MY_000248"
     CDS             13036..14676
                     /locus_tag="ACG8MY_000248"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0429482.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            14830..16152
                     /locus_tag="ACG8MY_000249"
     CDS             14830..16152
                     /locus_tag="ACG8MY_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0429483.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(16220..16654)
                     /locus_tag="ACG8MY_000250"
     CDS             complement(16220..16654)
                     /locus_tag="ACG8MY_000250"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0429484.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(16719..17555)
                     /locus_tag="ACG8MY_000251"
     CDS             complement(16719..17555)
                     /locus_tag="ACG8MY_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0429485.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(18297..18923)
                     /locus_tag="ACG8MY_000252"
     CDS             complement(18297..18923)
                     /locus_tag="ACG8MY_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429486.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(19218..19553)
                     /locus_tag="ACG8MY_000253"
     CDS             complement(19218..19553)
                     /locus_tag="ACG8MY_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0429487.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(19540..19824)
                     /locus_tag="ACG8MY_000254"
     CDS             complement(19540..19824)
                     /locus_tag="ACG8MY_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0429488.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(19880..21403)
                     /locus_tag="ACG8MY_000255"
     CDS             complement(19880..21403)
                     /locus_tag="ACG8MY_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0429489.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(21396..22571)
                     /locus_tag="ACG8MY_000256"
     CDS             complement(21396..22571)
                     /locus_tag="ACG8MY_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0429490.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(22575..22760)
                     /locus_tag="ACG8MY_000257"
     CDS             complement(22575..22760)
                     /locus_tag="ACG8MY_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429491.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(22726..24420)
                     /locus_tag="ACG8MY_000258"
     CDS             complement(22726..24420)
                     /locus_tag="ACG8MY_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0429492.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(24417..24890)
                     /locus_tag="ACG8MY_000259"
     CDS             complement(24417..24890)
                     /locus_tag="ACG8MY_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0429493.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(24959..25114)
                     /locus_tag="ACG8MY_000260"
     CDS             complement(24959..25114)
                     /locus_tag="ACG8MY_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFQ0429494.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(25248..25628)
                     /locus_tag="ACG8MY_000261"
     CDS             complement(25248..25628)
                     /locus_tag="ACG8MY_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0429495.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(25632..25847)
                     /locus_tag="ACG8MY_000262"
     CDS             complement(25632..25847)
                     /locus_tag="ACG8MY_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429496.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(25850..26257)
                     /locus_tag="ACG8MY_000263"
     CDS             complement(25850..26257)
                     /locus_tag="ACG8MY_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFQ0429497.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(26523..27998)
                     /locus_tag="ACG8MY_000264"
     CDS             complement(26523..27998)
                     /locus_tag="ACG8MY_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFQ0429498.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(27988..28836)
                     /locus_tag="ACG8MY_000265"
     CDS             complement(27988..28836)
                     /locus_tag="ACG8MY_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFQ0429499.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(28873..29235)
                     /locus_tag="ACG8MY_000266"
     CDS             complement(28873..29235)
                     /locus_tag="ACG8MY_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429500.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(29236..29394)
                     /locus_tag="ACG8MY_000267"
     CDS             complement(29236..29394)
                     /locus_tag="ACG8MY_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429501.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(29535..30159)
                     /locus_tag="ACG8MY_000268"
                     /pseudo
     CDS             complement(29535..30159)
                     /locus_tag="ACG8MY_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(30156..30467)
                     /locus_tag="ACG8MY_000269"
     CDS             complement(30156..30467)
                     /locus_tag="ACG8MY_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429502.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(30511..30804)
                     /locus_tag="ACG8MY_000270"
     CDS             complement(30511..30804)
                     /locus_tag="ACG8MY_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429503.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            30998..31645
                     /locus_tag="ACG8MY_000271"
     CDS             30998..31645
                     /locus_tag="ACG8MY_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0429504.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            31706..32851
                     /locus_tag="ACG8MY_000272"
     CDS             31706..32851
                     /locus_tag="ACG8MY_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0429505.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
BASE COUNT         9701 a         6804 c         5621 g        11229 t
ORIGIN      
        1 aatttgtatt tatgaaaaac tacctatata aaaaaaacaa gatataacga tcttcgtcat
       61 atcttgcttc ttcacctaac tatttttctc cctatataac agctttacgc ttctcctaat
      121 acaccattca aatataaatc gatcaattgc gccacattta gcggttcttc cacaggtctt
      181 ttcgaccgag tgatgtaagg tgctaacact aagaaaaaat gccttgctgc gatttctttt
      241 gaaatcccat ttcttagcac agattggttt gctgaaaaga tttcttcaaa aggttcacga
      301 taatttttct tccatagaaa gaaaagatga tctcttgttt cctttgaaag attgctttca
      361 agatcgtgaa gcatcagaaa caaatcgacc agatgtgtct caatcaaaaa ctgcgccatt
      421 tttgtcaagg ttacttgaaa tcccgtttcg cttgtttgaa cgatttctct caaagctttt
      481 ccaacatctt ttaagtggtt ttctaaaact gccgtatata gtttttcttt atcaccgaaa
      541 taatgataga gattgggttg ggtgatccca actttttgtg cgatctctct tgttgaagtg
      601 ccgagatatc ccttggacat aaacaagtca gtcgcttctt tcaaaatttt ttgctgtgta
      661 tctttgttgt atcgatggac catttttcca cctgcttttt ggttattctt tttctttcaa
      721 tacaccatct tccatcataa atattctatc gcatttgtca atcagccgtg tatcgtgagt
      781 aaccatgatc gttgctttat ttttgtcttt tgattctttt gccaagatat cgactacttc
      841 tcgtgccttt tctgaatcca aactagcagt tggttcatct gctagaataa tggacggatc
      901 attgtacaat gcacgagcga ttgcgaccct ttgccgttct ccaccagata gttcttctgg
      961 atacttgttg atcaacttat ccacgccaag ctgctcaaat aattcttttg cttctttttg
     1021 acgatgttca tgtttgatct tatctactaa taataactgc ttttttactg tcagaaaagg
     1081 aaccagattg gaagcttgca aaataaagcc aatttcttta aaacgtagtt taacgcgttc
     1141 actttctttt tgattactga attcttgatc attgataaat acagagccac tgctaggcgt
     1201 ctgcaatccg cctgccaaag tcaaaaaagt acttttacct gaaccagaag gaccgatgat
     1261 tgcaacaaat tcccctttat cgatactcat ggttgtttct ttcaatgctt taactacttg
     1321 gtcgccatct tggaaacttt tttctacttc tttaaattct attgctttca tgctcttcct
     1381 ccttcttatc caattgcctt caacggatcg atttttacga ttgtccgaac agaaaatagt
     1441 gcgcctaaca cagctactac cagcatcaac aagctaatga cgccaaaaaa cagccaattg
     1501 ctttggaatg gaaccgcatc aggtagaaca agggatgttc ccactgttcc taatagacca
     1561 agaagaatac caacaaatga taaaatgaat gtttgtgcca ctacagaacg cgcaataaat
     1621 cctcctgtga tcccttgtgc tttcattaca ccaaagatat taatcttttg catcgtcagt
     1681 acataaatga aaatcccgat cacaatagcc gcaataacga tcaaaaatcc gatcataaat
     1741 ccaaaagtca atacttgcgc attatatcca ggtaattcat tgatgaattt agaaattttg
     1801 atctgttcta aatcttcagg taaattactg attttacctc tgacaactat tgcgttaatc
     1861 cgtgcatttt cacttgtatc ttctttttcg aagcggatct cttggtaagc attgatggtt
     1921 gtatagagaa ctggtgagac attgaatttg gcatgatccg taaagccagt tagtttaaac
     1981 gtcttgtcac taccagataa cttaactgta tctcctacgg ctagaccata ttcttctttc
     2041 aaactaatat cgcctactgc ctcatcatcg ttatcaaaag cttttccttc aacaagattg
     2101 ggcatgatga attgattttt atcgataccg aagaagctaa cattgatttt tccttcttct
     2161 gtactgtctt tactagtcac gactccaggt gtctgggcaa gatacgccac tttcccacct
     2221 tcgacttctt ctgctatctt cttagtaatc atggacatcc ctagatttga atttgcatct
     2281 ttcgaaagca caatcgaatc tgcctcccac ttgtcgactg ctgttcgatt atcttgagct
     2341 aagccataag ctaacccagt caaaaaaaat accaggtagg cgatcaaaaa catcacccct
     2401 gcaactaacg cataacgtaa ctttgaatgc ataatttcat ttaatgctaa aaacatttaa
     2461 ctcctcctcc tgaaataatc attttataca acaattttat cacttgataa atttttggcg
     2521 aatattatgc tcaaaaaatt cttccgctat ttcattatat gaatttttta ttctcgattg
     2581 attaatattt attatatccc cacacaataa aagaccgtga tatttctcac agtcttttat
     2641 ctactctatt gctatttgct ctcttagcta gtactttaaa agctaactca ttgaggtagt
     2701 gaaaacgaga ggtgtgttca gacagaaatc caaaatcctc aactgctagc tgtggaatcg
     2761 tagttttgtg tttcacgata tccgcaagca ggttcaaatc tgataataag aattcttggc
     2821 ttatcagctg tccgccaatt atttctaatg tatccggatg gtagatcagc tttgcattta
     2881 ctgttttgtt ttcttcatcg tattgtggaa atagatcaaa ttctcctgaa cacgaaccgg
     2941 tattttgttc caagaatggc gcttcatctt ctgtcagccc tacagttcct aaaaagtagt
     3001 ctataatgac tgctgctgtt atcttttgtc tgaggtcgta agcggttggc tgaccactca
     3061 aagttagcgc tactgctctt gccatatgta cagctcgagt aactaaaggt aaaaagagat
     3121 gccgtttatt tttgtcaaaa ctgactggga cgatatctcc aatcgcatag atgtccggat
     3181 cacttgtctg cagatattca ttgactatca cagtctcatc ctcatacaaa gccacttttt
     3241 cttttaacaa tgtcgtatta ggacgagttt gggcagaaaa aatgaccata tctgccttta
     3301 ttgcttgaga tagaagttgt acggatacta ctttctcttc tgattttttc caatcgatca
     3361 agaattcatt tggataaaaa ttgatttgct tagtttgcag ctgattttga aaactgttga
     3421 tcatatcctt atcgaaatat cttgataaaa cattcggcat ataatcaact agatgaataa
     3481 tgtatccttt atttctcaaa gcatctgcta attccaatcc aatataacca gcaccgaaga
     3541 tcactacttc ttttgcttct tttaaagcgg caagtacttg cttagattgc actagatttt
     3601 tataggtgta gacattttca gcttcaattt gtttatacaa ggtgacagga gaggaacctg
     3661 ttgctacgat taatttatca taggttatgg tctcgttttt tccttctttg gatgcgagga
     3721 tgagctgatg tttgtctgga tcaagttcag ttactctcca ttcggtgatc agttttgccc
     3781 cactttcttg aagagtccgc atgttatggg caacttcagc taaactacgt atctttcctt
     3841 ttaaataaag gttgattcca tttgaaacat agcctagtcc atctttatga tcttcatcga
     3901 tcagtaaaac ttctgctttc ggttgaagtt ttcttagatt tagggcagct gtgatgcctg
     3961 catgggaagc accaatgatc acgattttca tgttttattc atccttaaat tcaatttttc
     4021 taaataacat tcttttaaaa attggttcaa ataggggaaa tcataattat attcgttgct
     4081 agtgaataca aaaactttag cggacttctg tgacgggtac tctttcaatt ggctagtcaa
     4141 aatgatatca tactgctttt ctttctcgta ttcgactaaa tcatatttga tcgtattcag
     4201 caattgttcc tggatgattt ctttcacgat atccgccatt agcctttcat aaggaagatc
     4261 ataagcaatt tgcagacgta attctaactg cttgatcagc gtccgcaaaa tagatgcata
     4321 gcgagcaaat aattcaaagc gggcattttc ctcgtctact tcagtaaaat caacaatagc
     4381 aaatgtttgt ctgaccaatc tttcacataa aaccaggaat tgtttatctt ctacttcagt
     4441 taaccgctgt tttaatcctt cttggccaaa aatattcatc catcctctaa aataggcaaa
     4501 atgagcgtga ttttgcatca ctgtcaacgc aatcttttgc gaaatctctt ctggaaaaac
     4561 ataaaaagag aaatattctt ctatcgattg tttaaacgtt cgattcaatt gagctactac
     4621 gtcgtctttg tatttttcgc ttatcaaaaa ctcctggaca tgcgagttat gtgtatctaa
     4681 tgtaaagtta ctgatcatga aaagataaaa aaccaacgtt tcattttgat tccaaggaaa
     4741 ggcatattga aagaagtaac gggtcaaaaa tgtatggagg tccaataaaa attgtgtcgt
     4801 ttgaaacaat ccaacaggaa gattgctata atctggtttg gattttttcg tacagcgcat
     4861 tcgcttcttc ataatggaaa gccatatttc aaatttcaat cgtccttctt ctgtaatagt
     4921 aaaactaaac tctttttgca gcagatctaa aaaagtgttt cctgataaat cactgtattc
     4981 ttttttgttt tcaagataag gacgtccaaa ccaaaataat tggaaataaa aataacaaat
     5041 ttgactttct ggaccggtca gccgcccgct ttttattttc agatgaaact catttaaaga
     5101 atcatttaat tccttcactc tgcgagacaa agtagccgaa ctgatattaa gttccatgac
     5161 aagtttctgc cgatcaaatg agccattttc caacagataa agaagtatct gatattttgt
     5221 agaatagcgc aagtagcaat gataataaac atcgactgct gctatatttc catgtctgat
     5281 taataaaata ttcccttctt ggataacaat ttccaattgt ttgatatttt ccaagttagc
     5341 ttgtaaataa aacagatctt ctttcaaggt agcccgatca actgataagc gggtaagtac
     5401 gttattaacg gaaatctctc ctttttcctc taataaaagt tggaaaagtt tgatttgccg
     5461 ttgttcattt ttttcaagca aactgaaaag ttccaagtcc actcctccaa gcatttctga
     5521 ttttaacagc agaagtttcc ttctcatcct tgaaaaagag gatctgtgac agttatcaca
     5581 gatccataca aattctaatc acttagctgg ttggcccagc tgtcagttcc catgtgacca
     5641 tgccttgata cgttgcgcca ggttgagcat tcgtactgct atttacctct aagagtaaac
     5701 catcggtatc gggaaagtca acgagaacct ctcctttagc tgttccatca ctctcataaa
     5761 tcaacgtgtt ttgaccttgc tgaatcaatt gttttgatcc agaatgattg aagtataatt
     5821 ttgttccgct taattcttga ccttgatcat tcttaaataa agaagtcaat gtccctgtca
     5881 gttgccaagg agacgtgctt ggaagccgag tatcccgcac atcaaaagaa aaatcgcctt
     5941 ttgcttcata ataacttgtc ttcaacggtt tggggagatc gttaccaaat gtaaaatcat
     6001 ctggtacatt aactaatgtt aattcttgtt tggggacaag aattgtttta gcagatgttt
     6061 ggacaactgg gttaggaagc ttagcaccat ctgctaatgt aatattgctg gaaagtacag
     6121 ctgcctttgt cagtatgctg acggattgta cagaagtatc tacagtattt aattgatagt
     6181 tgtaagtaag atgaatggta tctcctattt tgctcaatag atctgctggg acagaaactc
     6241 cttgttgaat gccggtatct gcgatagttg taccatttaa ttttaaggaa ccattgatat
     6301 aagtagtatt ttcaggaagt gagatcgtga tttttttgtc ggttatcgca tccccagatt
     6361 ttgttaacgt gaatgtcaca ttttcttgaa tcgtatctcc tgatagagca gacaggggat
     6421 ctcctgtctc aggttgtctg ctaaggtttt ttagctgact atctgcactc acagatggaa
     6481 aattagccac tgttatttga ggaagagtgg tgattccagc ttgttcagac tgccatttgt
     6541 acgcaaaaga cggatacatg ttagtaagta catcgtccac caagcgataa tcaaaataat
     6601 attgccctga agccaatgca ggtagtgtag aagtagctgt atttacaccc ccgttatttg
     6661 attggatcgt ttggaaaagt tgatctgtcc atcctgtcgc taatttttgg gttcctttcg
     6721 aataaagcct gtaatggatc gaaccctttt ttagttcagt tgctgaccaa ttggccgtga
     6781 cttggtacga tgcagaagaa aggagctttc cccactgact atcggctgtt aagtttgttg
     6841 gtaaaaccgt tccgtctagc ttgtactgct ccaatacctt taaagcaaca ggagaaagca
     6901 atccgccttg cgcatctttc ccactgtaca cgatattggc tgtctgattc aatccacttg
     6961 tgcttgctgg agtatgagtc agtattgctc cttgtgtaac gttttttcct gtaaaatagt
     7021 tgactacgcg atcaaagcga ctaaatggca actgatcaaa acgtccttca gtcagcccca
     7081 tcgtcaatgt ccgactattg gtagagcctg gaaggttctg gttatttcca tccacgtatt
     7141 ctgtggtatc agcaaatgta ttatattttc ctgttgaatc acttgttgag atgtaactat
     7201 tccacgtatt tatctctgca cttgcagaaa atgcatatgc tcctggattc aagtttccgc
     7261 cagttaattt aggataaaat ctacctgcag ctggagctaa aatcgaattg aacatacgat
     7321 cttggataat attattcgaa tcataaaaat caacagaaaa aacggccttt ttagacatag
     7381 aaggttgtaa tttacccttg gtcaaaattt tgtttggact cgtatcatat ttatctccgt
     7441 tttctgtcat cttcgcgaca ttagctgtaa catcaaattg aagcgggata tcgtccacgt
     7501 tgaattcgcc tcgcgtaata atagcaataa tatctgccaa tagagtattt gtatcccacg
     7561 accccatccc aaaacgcaag gtatggcttc ctgctgataa taaactaccg ctagtaacct
     7621 tgatgggggc accggcaata tacaagtagg cgttgttcca gtcaattgct tttaatacat
     7681 cactgcttga tacatgttcc ggcaaattga tgatcgccac aaatgcaaat ccttgtgcag
     7741 tgatattcac caaatcaagt acatttgtta gtttcacatg gctttcccaa tgaaaacgga
     7801 ccgtgccacc tttccaagga tcatttgaac catcgttcaa ataagtagca gtcaatttag
     7861 gcaattggtt agccccagtt agttggacaa gcttcccgcc attgactgca ctacgtccac
     7921 catttggagg tacgatattt tcatacgtgt atggatcata agaaatggca ggaggcgttg
     7981 ctccaacagc ttctgccgaa ggtgaaaaga taaatatact taaaacaaaa agcgaagtaa
     8041 aaaagaagga gcgaaaaaat gaaaatttat tttgtagcaa catgttcatc ctccttttcc
     8101 tttcttctcc ataacaacaa ccacgcaaga agccataatt ctataccaat gatcaaataa
     8161 agaaaacgga tcttttcttc attcgtctcc gggaatacgc catgttgatt agattcttta
     8221 taaatcggta gctgtgtctg ctgttttgat gcgccattga ctgaaccact tggatgcgga
     8281 actggactta gatcataaaa tgtaatcgta cctgaacttt tggtatctgc ttggactggc
     8341 tcttgccagc tgaagaacag caaacttgca ataatccctc ccaataagat cgatgttttc
     8401 aacgaaatca cctcatcatg ttgtttctcc ttttttcttt ttcttgtgga cccacaaaaa
     8461 cagcaaaatc aaccataaca ctagaactgc agcaaccaat cctgcataaa gccatgggaa
     8521 agattgttct tgtactgcaa aaacggaatt ttcatttact ttttgtgctt tttctttttt
     8581 gatcgtaaag ttttgtgtaa atcgccattc attactgtcg cctgattttg ctatgatttc
     8641 tgcgcggtaa tcgccggctt ttgctttatc accagctaaa tcaagattca agtgaaaatt
     8701 cgaatttggt gcaactaaaa attcattaga cgtattttca atataaagct gattttcata
     8761 aaagatcttg ctcgtaaata tcaaattgct gataatccta ggctgaggat tttggaaacg
     8821 aagctgaaca gttggctttg cgttgacctc gtccaaatct cctgataaaa gtttcagatc
     8881 cggttctatc gtctcttgac tttcttgtaa aacgactgcg agattataag caatacgatt
     8941 tttgatttgt gctccttctg tagcattttc tgtttcagct tcttctttag gtgaaacatg
     9001 aatacccgct aatacacgtc ccttaaagct ttttgctggg attttcactt ctataggtat
     9061 agttgtttct gcatgtgctg atacattgat ttcctttttc gcaatgagca aaacgtcacg
     9121 gacatcaaac ggcaatgtct catctaacgt tgggtttttt tgactataat cgatcgttcc
     9181 accatcactc gttaccgccg gattgacaga gacctcaaat gttcgttctt catctgaaga
     9241 attgctgata attacttcta gtgtctgatt ttgttccggt gccactaata aatcaaaata
     9301 accgattgct ttatctattt ggttctctgg aaataccggt ttgattccaa agtctcctgc
     9361 tgaatccttt gcttggctga ttgacggaaa aatgaaaaat agacttaaaa tagaaaaaaa
     9421 cgaaacaaac agtcgtttac tttgattcat tgtccaccac atcctttcaa caacaactgc
     9481 ggtaaaaaat ccttcaccta tagtattttc accgcagttt gttgaattta tttcatactc
     9541 tatgctactg ggccagccac gaggttccaa gtcaaatcag ccgtgaatgt tccagctgct
     9601 gctttcggca ctttcagtgt gatatttgct ggatcataaa cagaaagcca tgttcccaga
     9661 ccttgatctt ttgctgctgt aaatattggt gtcgctgcac ttgctttact caacccagtt
     9721 acagtttgac tagttggtcc agtggattcg ttatctgacg tacttttaac agtggatgtt
     9781 ccaaaatcta attcagcgcc ttgcaaaact tgtgaaccgt ttacaaaatc tgaaaccgaa
     9841 actgtcacgt tccacccttg tgcttggcca tctgcaccta cacctcgacg atctgatact
     9901 tggagataag gtgtatcgtt ttttgaagcg tctacttgat acgtttttgt tccgctttca
     9961 atctcgtgtg taccaaatgg caattcagga actacatcca atgttaaaga acctgtctgg
    10021 ccagttgctg gattttccgg atctgctggc tctactggtt ttgaaggatc agtagggtta
    10081 agaggctcaa cggtatttgt tggttcagta aaggtaatat ctccttttga agtcgcttga
    10141 ccctcagcaa aagctggtgt tgccaagccg cctagaagaa gtgttgaaag taataatgtc
    10201 ccaatcatgt tctttttcat aataaatcac tcctttttct tagtgaccta agtatagtag
    10261 agacagtatg caaaaaatcc tcatgaattc atccgcttac ttgaaattat gacccttttt
    10321 gtataaaaga gaaaaaaaca ataaaatcgt ttacatgaat attcttcctt ttttattaag
    10381 tatgaaacaa tatttcaatt ttattttttc gtttcaaatt attctattag tgacatcctt
    10441 gaactataaa aataggagta cggcatcatc tgccgcactc ctattttttt cgtcatttat
    10501 ttcttaaata aaacgattct cacgcaattt tatttttttg atatcttcta ctgtttgtgt
    10561 tccagctaat tgcatgacca tttccaattc tgttttaaag aaatcaaaca cttgatggac
    10621 tcctgtactt cctcctaaag acaatccata aataacgggt ctgccaatag caacaagatc
    10681 ggcacctgat gcgattgctt tgaaaacatg ttgtccacgt cgtacaccgc tgtcaaacac
    10741 gattggtaca cgtttatcta ctgcttctgc cacatattgc aacgaatcaa acgcagcagg
    10801 ccccccatct aattgacggc caccatggtt agatacccaa attccaccag caccagattc
    10861 taatgatcga tagacatctt cttctgattg aacacctttc acgtagactg gcaaatcaga
    10921 atgagcagcg ataaattcta catcttttgg actaagtttt tgtttggaag atttatatac
    10981 agcatccatc gtttggccta ctcctgattg ataagcctga acgatcggca ttggcagcgg
    11041 gaaggtaaac ccgtttctgc ggtctgtttc acggtttccg ccaactgttg catccgctgt
    11101 caatacgatt gcttttgctc cattacgctt ggccacttca agaatatcca aattgattcc
    11161 attatctttg ctcatataaa attgaaacca ttgcggtgct ttctccccgc ctgcttctct
    11221 tatctcttct aaagtacaag aagcgtaaga acttgctgta tagatcgtcc caaaatctgc
    11281 tacacctttt gcagaagctt tctccgcctt cacatgagct aatccatgag cagcaactgg
    11341 tgccatgatg attggtgcag tcagcatttc ttcgtcaaaa tgtgtagttg tatcgggtaa
    11401 ttctacatct cttaatacat gaggaataat caaacgatga ttgaatgccc gttcattttc
    11461 ttgataagta aataagtcgc cagcaccact gctgatgtaa ccataaccac cagctggaat
    11521 cacttgtgaa gccgcgattt ctaaatcttc catattgata aaatctacta ttccttcatt
    11581 tgtacctgct tgatatgttt tttccatctc aatgccttct ctctataaaa tttgaatgat
    11641 gaacaatatg atagcgttta cactttttat attactcttc gacattgata caggcaaata
    11701 atctgtttac atttcagcca ttccagatgt cactactctg gaagaaatga ttgtaataaa
    11761 aatagataaa aaaccgatgg gactttgaca aaagtagtgt caaagtccct ttttttgaat
    11821 aaacggtgtt caaaagccat tttctcggta ttaagtcaaa tttatgcaaa aatgtggaaa
    11881 gccattttca caaaattttt cttactactc gaaactaagc gactttttca caacctctcg
    11941 ataaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaatggc aaaatatgag
    12001 gaactatttt gccatttgtc tctctaatac tcaggtcaga acgttgactt cacagtctct
    12061 tccttaaacg gtgttcaaaa acttgcatct gcggtaataa gcccaaccaa acgaaaaata
    12121 ggaggagcta tttttgtttg gctgttctta ttacttgatg cagggcaggt ttttcacgac
    12181 ctctgattca cggtgtttct ttttcatcaa catttttacg aaatactgtg gaagactatt
    12241 gaaataagtg tggattgttt ctttgtgttt caatcgtttc ttgccattct tcatttaatt
    12301 ttggattacc gttttcttcc ttgaactcat aaggtcctaa ttcaaaaatc ccagacagta
    12361 tttgattcac gatttcatat agttcagtgt gatcagacac atgattcttg aagatcatct
    12421 cattatcacc acgtttgaac ctcacattcg ggctctcatg atgaacctct atgcattttt
    12481 ctcctttttt taactgacgg acttctgaaa acatctcata aatatcttct ccagtaaaac
    12541 cttcagcaag cgccagactg tccacaagac cgcctactgt cagttcagag attattgctt
    12601 gattggaatc taattttaat gtatagcttg tcgcttctac tacgccacat gcattgcaac
    12661 cgccttctga tagttcgctt tttctaatca attgaacttc tttcattttc tcttcctcct
    12721 cttaattttc tacttatgta tttttatcac ctgacatagg tgactcctat caacgctatc
    12781 atgtgctttt ttcctcagaa atcctattta tccattccat ctatccctta ttcttctact
    12841 tttctttctc tagcatataa gagatacgca tagtattcaa gcaaggattg tcgatataaa
    12901 aagaacaaat actgctattt tgatttcttt tggctatatt acatataaat aaaatttatc
    12961 atacctgctc tttttgacta tatcatcatc tgacttctat acttcgtttg tattaaaact
    13021 taggaggaat cattcatgct tacagggtta aacttattaa ataatccttt tctaaataaa
    13081 ggaacagctt ttacaaaaga agaacgggca aaatatggaa tcactggtat gcttccaagt
    13141 actgtccaaa cactggaaca acaatctgtt caagcttatg gtcaatactt aagcaaacaa
    13201 acagatttgg aaaaacgtat cttcttaatg aatctattca atacgaatcg gacattgttt
    13261 tataaattga tgggcgaaca tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt
    13321 gcggattcaa tcgaacaata caacgaaatt ttcttgaaac cgcaagatgc tgcatttctt
    13381 tcaatcgacg aaccagaaaa catcaaagca agtttgaaga acgcggcaga tggacgagac
    13441 attcgtttga ttgttgtgac tgatgcagaa ggaatcttag gtatgggcga ctggggtgtg
    13501 aacggtgttg acatcgctat cggcaaattg atggtctata ctgctgctgc tggtattaat
    13561 ccagcacaag ttttgcctgt atcgatcgac gctggtacta ataatgaaga attattgaat
    13621 aatcccttat acttaggcaa tcgtcatgct cgtgtagaag gcgaaactta ctatgaattc
    13681 atcgatcaat tcgttcaatc agctaccgaa ttattcccag aattgttatt gcattgggaa
    13741 gattttggac gaggaaatgc tgcagcgatc ttagaaaaat acgaagatca aatcactact
    13801 ttcaacgacg atatccaagg tacaggaatt gttgtcctag caggtgtatt aggcggattg
    13861 aatatatccg gtgaacaatt gaaagatcaa ataattttga catttggtgc tggtactgct
    13921 ggtgtgggta tcgcaaacat ccttttagat gagatgatcc gtcaaggtgt acctgaagaa
    13981 gaagcacgcc aacatttcta tcaagtagat aaacaaggtt tattgtttga agatacagaa
    14041 ggattgactc ctggtcaaat cccattcgct cgcaaacgct cagatttcac taatgctgat
    14101 gaattgacaa acttagaagc agttgtaaaa gcgatccatc cgacaatcat gattggtact
    14161 tctacccaac ctggagcatt cactgaagaa atcgtgaaag aaatggctgc tcatacacca
    14221 cgtccgatca tcatgccttt gtctaatcca acaaaattgg cagaagcaaa agcaaaagat
    14281 ttaatagaat ggacggacgg aaaagcatta gttggaacag gtatccctgc tgctgatgtt
    14341 gaatataatg gtgtaactta ccaaatcggt caagcaaata atgctttgat gtacccaggt
    14401 ttaggacttg gtttgattgc ttcaacagct acacgagtaa atgctgaaat catttctcaa
    14461 gctagtcgag cattaggcgg aatcgttgat gtaacaaaac caggagcagc tatcttgcct
    14521 ccagtagcaa aaatcactga attttctcaa acgattgctg aaactgtcgc aaaatcagtt
    14581 gtcgcacaaa acttgaatcg tgaagaaatc acggatatca aagaagcagt tgaatctgct
    14641 aaatgggttc ctgaatataa atctttagaa gattaagatc gttatagtaa aagaacatac
    14701 agaaactttg tatgacgtct aaaaagaaaa aagcgactgc ggcaaaacag cggccagcca
    14761 actcctgctc ttttgtcgct tttctttggc ttttttcata tgatacagaa taagaaagga
    14821 tctgtaaaaa tggcacaaaa aacaacaact gcaactgcag ttgaggaaaa gaaaaaagaa
    14881 ggtttttggt ccacaaaaat caccggcgta agtttgccac tttatttgat catggtcctt
    14941 gtcctgatca ttgcaatggc tttagggaaa ttaccgacga atatgattgg accaatcgca
    15001 gttttggtta ttttcggtaa tttatttcat tttatcggga caaaaatccc tatcgtcaaa
    15061 agttatttgg gtggtggttc cgtttttgcg atcttcgctt ctgctgcgat tgctacattt
    15121 gggatcttac ctgaatatgt agtgaaatcc acagaaaact ttgtcagcaa tatgggcttt
    15181 tgggactttt atattgctgc cttgattact ggtagcatcc ttggtatgaa ccgtaatcta
    15241 ttgatgaaag cttctgttcg ttttattccc gttgcactta tttcaatggt cgtctccttt
    15301 tttgccgtag gactcgtggg catgcttatc ggaaatggtt ttgcaaattc cgttttatac
    15361 atctcattac ctgcgatggc tggtggtgtt ggagccggag ccgttccttt atcaacgatt
    15421 tatgcaaatg ttttaggtac ggatgcttct tctatcattt cccgcctgat tcctgcgaca
    15481 gcaatgacaa atgtcttagc tatcatcggt gcagcattag ttgctcgtgc tggacaatca
    15541 atgccaaaat tcaacggcaa tggtaaatta atggaaaaag gcgatcttgg cgatggaaaa
    15601 cctcgaactg tgaaaccttc cattccacag ttaggtgttg gtttgatggt atcattgact
    15661 ttcttcatct taggacaaat tggtaactat tttgttccta aagtccatgc ttacgcattt
    15721 atgattatta tcgttgtcat ctgtaaaatc gccaacctat taccagagta ttatgaagat
    15781 gcagctatca tgttcaataa cttgattgtc aaaaacttga cagcagctgt tcttgccggt
    15841 atcggtattg cgttgctgaa tctgaatgta ttagcttctg ctttgacatg gcaatttgtt
    15901 gtcttatgtc tgacaagtgt tatcgcgatt tcagttgttt caggatttgt cggtcgtttg
    15961 tttggtttat atccaattga atcaactatt actgctggct tatgcaacaa ctcgatgggc
    16021 ggaacaggaa atgtagccgt cttatctgca gctaatcgga tggaacttat tgcttttgct
    16081 caaatgggca atcgacttgg tggtgccatc gttttgatca tctctggttt cttgatgcaa
    16141 ttactttctt agtcaaaaaa acaaaaatac ctgatcatca agtatcggct tggtgatcgg
    16201 gtattttttt gataaagatt tacaggattt tcaaaaatct tttttgcatt ttatcggaat
    16261 gataaccgat gtgttcataa aattggtgtg cttctactct gtattctgca gaattaagcc
    16321 ttattgtcgt tattcctcgt tttttcgctt cttcttctac gccagtcatc aaagcctttc
    16381 cgatgcctct tttttcagca tatttagaca cagcaagtgc cataatattg aacattggat
    16441 cgctaaaaat cgattcatag gactccgctt gtacatagcc agctgctttt ttagaagtag
    16501 gatcttcata aaccaagaaa aaatttttct ctggctcctt cagcagtttc ttcatttgtc
    16561 tcaccgtaac ggttaatggt atatcatacc ctaattgatc cgcattgatt tcacgtagtg
    16621 cttctgcatc ttctaaacgc atttcccgta acatccaaat caccagccaa tttctgattt
    16681 tttaaaatga atttttttat catagtatac tctttttttc atttagaaca atatgtttca
    16741 gtgattttga cttgcgttag tctagattct cctaatgttt gtaagatgac aaaggcattg
    16801 taacaattga ccaaaagaac cacgaggaca attacttgaa aaataaaggt cacttttttt
    16861 ggagacaaaa ccctgctaaa tcttgctccc cacaaacctc caataacagc agcggggata
    16921 acataaaaca acatagataa atcaaagcgc ataaagctac ttgttaaagc aatcgttacc
    16981 aattttgcca gttgagaaaa gaaaatcgta gatagagaat atagtgttgc ttcttttatt
    17041 ggcaatgcaa acatcagcat caacaaagaa acattgactg gtccaccacc aatacctaaa
    17101 aaactagcta aaaagcccag aaccccccca catataaggt accaagtcca tgacgtcaaa
    17161 tgaaattttg gctgatgatg cttggtgtag aagaacgcaa aaaccaaggt caataccgtc
    17221 aaaaaaattt ggatcagctg cacatgcttc tcgttttcaa ataactgtaa aaaaacttcg
    17281 aatacaatgt tgccgactac tcctccaaca acagctccac tagataccca accgacaatc
    17341 tgccaattca gtgatttccc gcttgatact tgtcgcacag tcgaaacaat ggacatcgtg
    17401 aataccgcaa ctgttgagta aaaagaaatc cctgcaacag aatgggcgcc gataagatca
    17461 agaatcggtt tgatcaacac gcctccaccc atcccggaaa tagcgccaac tgtgttcgcc
    17521 agaacgatca ccacaaaata aatcagacca atcatcttgt atttcctttc tcttgtttat
    17581 atcttttagc gattgttact cttttctttt attatacagt aaaagaaaca aacgaggctt
    17641 cgaaagcttt tctacttttt aaggaaattt agtgctaaac gaaaagaggt tgtgaaaaag
    17701 ctgtcctgca tcaagtaata agaacagcca aacaaaaata gctcctcata attttcgttt
    17761 ggttgaactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag aggtcgtgag
    17821 cctgccgttc agctccgagt cacaaggaac aatttcaaaa aacagcttct catgtttgtt
    17881 gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttaagg aagagactgt
    17941 gaagtcaacg ttttgacctg attattagag aaacaaatgg caaaatagtt cttcatattt
    18001 cgccgttttt cgagcgaatg ccgaggggca gctgattgaa catcgtttat ttggaaaaag
    18061 ggattgtgac aagacgtttg tcacaatccc tttttttata tcttattaat tcattctttt
    18121 gttctattct tcttcttccg ccatgaatga gaaacccata tcaaagaact ataaaggtta
    18181 tcaaaacagc gtttatgact aaatgaatta gagttatcta tcactatttt tcaagcgatt
    18241 taaaccaaat ggcatacaat atggcataca aaaacttcaa taagccgtgg gggtttttaa
    18301 atttcttctt gttctttaaa atcaatgagt ggagctacat aatttgaatc cactttttca
    18361 aacgtcactt tttgcatcga cgaatgtttt ctttcccaag ataacgtaaa tagatttgac
    18421 tctggactta taaagtctac ttcaccttct agtattactt tcttatgcaa atattcctct
    18481 aataaatata gacttgctaa agcatacaac acattttcca aatttgcttc cttataattt
    18541 gaagaacgac catgttttac tctgttatat attttccacc aatctggtgt atcttcaggc
    18601 cacccatctc caaaaggttt tagtgaagtc aatgttaaag aattcataac cctaacacca
    18661 ttatccaaac acattaattc gctagattta ataacagctt taaaatcagt tatgctttta
    18721 ttgctactgc tatcaaatcc gcaaagttct ttgaaaataa tctcacactc cgaaccaatc
    18781 gctagtagta gtttttgata ttcaatagag aatgttttga aattattctc atcgagcatc
    18841 acaaaatctg tggtattaat aaactcattt tctaaatgta gataatactt ccaataaaaa
    18901 tttgtaaact ctgctttgtc cattaaatca cctcttttga attataacaa aaaaaacacg
    18961 tccacaacag acgtgttctt tgatttctac acaatcagct tgccaaaacc atccaatcag
    19021 tcctagcatg cctgtattaa aattgaacgt tcatcatcta tcatctttct tagtccagca
    19081 tgtttgcaac cacacctaca attttcagtc caacaataga tttcgactag ctaagtcttg
    19141 caaaattcag gcagctaacc cttcatttac taccttaatt atactaaata cccacaacgt
    19201 ttacaagtgt aatcattcta agaaatctgt ttagctatga tcgttgtata atctttaaac
    19261 gccgttcctt cattgattga aataatttta taactcttgt ttttccaagc tactttcata
    19321 tcgtttgtta gttcataatc ttgatcgtat cgaataataa acgtcaacgt tccttcaaga
    19381 atggtaccta cactagcaag cacgtctttt agcatttgtg aacgcacatt agcccaacat
    19441 tcaaatacag tttcttcact atccaccatc tgcccatctt catcttttac ccgttttgtc
    19501 tttttaaact tgactctctc agtcaattca tttacattct taatgattgg cataataagc
    19561 acctctcatt tgttgtacta atgcttgtac cgttactggt atccgttcgc tgctggcttc
    19621 ttgacggttc aaataccaat gttgagtaag taatgacacc gccaaatcaa attgctgata
    19681 attatccata tctttatccg ttgcgctact atcaatagca ctttttatat attcgcctgc
    19741 tgtatcaatc aattgttgaa taaagtcatc atcaatagtg tgatcgatcc gcatactttt
    19801 ttttatgcta tccaaaatta ccattttatc tctcctaatg aaaaaaaggc agtgggataa
    19861 accgccgccc aatatttttc tatacttttg gtgttacaac tggcgtaaac tcaatataca
    19921 ctgaagcatt ttgatcaatt ttcttataat cgttgcgaac gatcactgca agtccttgtg
    19981 agtagtaatc gaatttttcc cattgagtcg ttacttgatt tctacgagca acaaagatag
    20041 attgtgcaat atcccccata atcataggga atgttccagc tttaggattg gcaaacaatg
    20101 tatctgcaat caatactact ggcataccga ataatgattt accactaggt gctgtcacat
    20161 cgggttgtaa aatgtaacgt ccatctgaat ctttcaatgt atctaggtgg taatatccgc
    20221 tttggtttag aattaccatt ttatttaatg ctgggtctaa tgccacattg tatagttgtt
    20281 tcaaatcgtc caacgtagca gccgttttct tagtgaatgt ttttaataaa tccatgatat
    20341 gcttattgtc agtgttttct accaatttcg ctaactgatc tttgacctct tgtacaatat
    20401 tcactgctga atcttccaca acctcatttg ataaggcaat cttaccagca cgagtttcta
    20461 ctttataatc aactgaagta aacatttcag cgtcaatatc accaatttca gcaagttcag
    20521 cttttgttgc taacactgct tgttggttag ttgctactgg atatttacct tgtccatttg
    20581 atactgtttt tactgttgca tattgagcta agttataatt tgaacgtttc aaatcaaaga
    20641 cttcaccgat tacttcttcg ggaacaactg ccgctgcatt tacagtagtg actccatcac
    20701 gcacttcgcc ttgtgaacgg atatattctt catacccacg caattctgtt ttttcgttat
    20761 cgattaaagt tttttccatg ttattactct ccttttgtgc tttagaagtg gattctaagc
    20821 tattgtttgt ttgttttgct tggttgttac tcataaacga ttcgtaagaa cgcttattta
    20881 cttggacatt tgatgaatca taagcgggaa cggtcacgac actaatttca ttaagtgctt
    20941 tgattttatc tattgatcgt tcaattgtgc catcttcttt tttgtcgaat gaatctaatc
    21001 caagcacgaa cccaaaggac atagaatcaa caaccccttt tgagatattc tcatacacat
    21061 catttgaata ggtcgtgtcg gttagctctg cttcaaaatg taatcctgta tcatcgacat
    21121 tcaatttcaa cgtacctgct ttgacactag ctagtggctt tgaataatcg tgtccatata
    21181 ataagaacac atttgataaa tccacctctt ttaatgcttc gggtgtaatc acttctacaa
    21241 agccgcctaa atcttttgac ggttgcccga atttcaacgc atagcctgaa atagttttgc
    21301 cgtctgttgg ttgtttttca ttttctttct ctactttggg agaatcggct gttagctcag
    21361 cttcttctgt cagccgtttt tccttttctt cttcattatt caactggtgt aactccttcc
    21421 tgttctttta attgatattt ctccaacgta tctaaatacg taaagttcaa acttgctagt
    21481 aaacgatcgc caccatccat ggggggtagc cccattttcg accgcccctc attaatcgtg
    21541 agaagtgacc ctttgacctg atccaataca ttttttactt ttgtttcagg atcagtctct
    21601 agtaactgat cggaattaaa gcggatattg gtttcaagtt tcgtatctaa ttcactcaca
    21661 aacacattaa agtaatgaat cagcgtactt tggatatact ggagattact ttgaacggtg
    21721 ctagaatgtt cattttcaac gcctaaacgc tcaatcggta caccaaacgc cttggcaatt
    21781 tgttttgtgt tccaatcatt ggaattgata agcttcaaaa catcggtgtt tatttctaat
    21841 gttttgtagt ccattgtttc atcaagaatg atcgttctaa gagcattatc tccactactt
    21901 gaaccgttgg cttcttcaaa cttttcacga attgcgttct ttgctgatcc gtctaaatct
    21961 gacttatgaa ccttcaaaat acctgaccca ctgacaccac gagtaaagaa attgtgcaat
    22021 gtgcggttgc cagcttgttg aattttcagt tcatcacgta acgcataaag tggtggtaat
    22081 cctgttaaac catcttgggt gaaatatttg aagtgcaaaa tatcgctaga cttcacacgt
    22141 ctttttttat caccaatctg ataagacaac gtaccattat ctaattgagt aaccgttaca
    22201 cttgagttag gtaataagtg aatttcttct accttttcac cgttgcgttt aatctctgca
    22261 aagctattac cgtttagcaa catattgacc gccaaagcaa actttaaatg ccaaccatcc
    22321 atttctgaat taggtttctc gtttagtaac ttcaccagtt cattatcagc ttgtggcata
    22381 ttgtttttga ccaattgaat cggacttgaa gcaatatcac tagcaataat tcgaacggct
    22441 gcaaacacat cactatttct taatgcacga acgcttgtaa agctggtggt gtaatcatcc
    22501 gattggattg ataccacatg ttgtaggaaa ggatctcccg ttgtattttg tctcgcttta
    22561 aaaaatgcca tcttttaacc tcccttctgt tctgtattta gcatgaccgc taccccaata
    22621 agcactatgc ccagcgctaa caagccatag tagacatttg ttagtaaggt gatagcaacc
    22681 aacacacaca ccaatccaag aagtaaaaga acggtatgaa tgtaattaga aagaaaattc
    22741 ttcacttgta taaaattcat tgtctgcttt cgctccttcc gttgtggtga agtaatccat
    22801 tgcaaacaca taggcattga ttaatgcagc tattggatca attttgttac tgttctttga
    22861 tttattaatc tgaatgccgt tgttatccgt ttttaaaata gcgttgttca ctgcatgggt
    22921 gagaatcgta tttttattgt gaataacgtt gccttcataa acttgttctc taaaagttcg
    22981 ggtcggaacg tttagagtaa tcgttccttg tcttacttct aacattgggt agttggcttt
    23041 ttcagcttta ctgataagcg aattagcgtt atacgggtca taacaaatcc ccatacattc
    23101 caaatcattt tcttgaatca aatcttgaat aaactgaaat acttggtcat agtccacgat
    23161 tccactttct aattgagtaa tggaacactc accagctttt tctagttctc gataaggcaa
    23221 accgtcgcgc ttttctttgt cttgaagccc atatttggtg gctacaaagc tatgtgaatc
    23281 acaataaagt tcgccgttat ctagcggtac gatccatgaa acgctagtta aatcatctgt
    23341 tttagataaa tccactccga tataaacggg cttgcctgta atatcttgtg gttctacttc
    23401 ggttgcctgc cagtcttcag caatcatata gctgtcctca ctcgcttgtc tccataaatt
    23461 gaagtttttc accaatacag aattaaggtt attttgtttc aaagcaaggt tcacatcatc
    23521 ttgaatggtt ggaatcatta cttttttgat ttcttcactt tcaaaaatcg gattggcttt
    23581 gatccatttc tcttgatcgt gaatttcttc gggatcgtcc aattcccata tcgctataaa
    23641 gtacctgtcg gcttgttctt cgccttttag cacacgatct aacaataagt attcctcgta
    23701 cataggaacg tttaaatcaa gcccagcggt actaataaca cacaataagc cgttcttttg
    23761 ttgggtcatt cctgacttaa tgacgttgta cgttttacga gtttttgctt cgtgccactc
    23821 gtcaagaatt gcggttgttg gtgcaaaccc atctagcgtg ctggtttcac tagccagtgc
    23881 catagcgaac gaattagagg gcaagtgctg aatcttagag ttcataattt tcagttgtgg
    23941 tcttaaaaac ttactagact tgaccacgtt tctaagtgaa ttagacagca tatcatagcc
    24001 caatttagct tgtttcaaag cgttacttac aaacaatact tgccttgctt ctgctggttc
    24061 ttgttccata atcaacgcat tggcagccat gccacttgcg atatacgttt tcccattctt
    24121 acggctcata ctggtaaacg ctcgattgaa tcgcctgtaa ttcccttctt tagtacgcca
    24181 gccataaaga ctgcctagaa tgaatttttg aaatcctaac atcttgattg ctttaccgtc
    24241 cgtagatggt agcaactcaa tgaatttaat cgctttggtg gcttgttctt catcgaacac
    24301 ataagggaag ctatctgact tagaacgctc caaatcgctt atatgacgct cacacgcttg
    24361 gataattttc ttgggtgcaa tcagcttacc tgataggact ttctcaatgt aatcattcat
    24421 gaattcatca actctttgaa cgggtcttcg ggttcatcat ttttaacctt gttcaaagct
    24481 agttttgatc gtgattctaa ggtcatacct agtgaggtgg cttgtttttt caaatcactc
    24541 atagcttgtg attgaacccg aaccgctgga ttggctttct ttactccgtt ttcgcatgtg
    24601 ataaacgtac ctgattttct tatctcggct tcggctgttt tcattcgagc aaaggctaaa
    24661 caataggcta ccaatgaacc ataatctgct tcactcaatg gataatcttt ttttaatgtt
    24721 ggtacgatac gattccattc acttctagcg ctcgctgcca tccagtcagg gggcgtgata
    24781 tttattaatg gttgctgctt gaacaattct tctctagcgt ccacacggat tgctttttct
    24841 tcacttgata tattgccttt tgtatcgtgt aatattttag ggtgtcccat gttgtcctcc
    24901 tttcagacaa aagaaaaagg aacgtaagca attaagctca cattccttcg tttttcgatc
    24961 agaatttttc tttatctaat ttatctatat aaatgatttt tccgttctgc atttgaatgg
    25021 tcacttgacc aaaatgaggt aactcaatgg tgcgctgttt ccctgtttta tcagtaataa
    25081 caattgtatt tttcaataaa tctcctactt tcattatata ctttttcact tgtgattaca
    25141 agtgtaatcg ttatttgtaa ttacccttat gccttttcac aacggttatt tgtaccaaaa
    25201 tgagtaggca tcgattgtca gtaagtttca cactatgccc ccttaactca aagggtactc
    25261 ttttctcgtc tttgtctttc attggctgtc ttggaattat ggtgtgcatg gcataaagaa
    25321 cgaaaattag tttcatctaa ccgtctattc caatcatctt ttagttcaat tttgtgatct
    25381 acaacatcag ctttccttat caagccttct tctaggcaat cctcacatac tggatgattt
    25441 aaccgataca agtatgaagc ctttcgccat gttctacttt gatagaattg aaaatattta
    25501 ccttcgcttt gctttctttc ttcacgctta attgccgttt gctttgcttt atgcttccca
    25561 caatacttct gcctataatc aatcaatgtt ttacatccag catgattaca ttgtttttta
    25621 actgccatag attaccaacc aaagcgatct cgttcgtcag aatcatcttg ttcccatttt
    25681 gatttgtatt caatataagg cgaaactatc ttcaagcacg ttccgtcttt aaaattaact
    25741 ttagtgattt tatcttcctc aaaactaata aagtctacat cttcttgagt gtttgaatac
    25801 tcaatgtgtt tctcaccatc gaagtatcta atactttcca tatgcataat tattctacct
    25861 ccaataaatt agttatcaat tcatcatctg ttagtagcaa agccaagaac ttatcatgga
    25921 tgtttaaaaa caaattatcc tcattcgatg taagctctcg ttgtctcgct cgatcatata
    25981 ggttaagcag acaattaaat tcatccatta gctcacctgt atcatcatag ttgccactat
    26041 ctaccaatga tcccactact agcgattgac accacgctac tgactcctgc agccaatcag
    26101 gttcttcttc cattatcttt aatacttctg gatcaaatgt ggaataatct tctgttacca
    26161 atcccacact aaatgccagc gtgttcatta gaaaccgatc catttcatct gtatattgaa
    26221 agttcggtgc tttgtttgtt gaaataatat tgagcattta ctttcctcct tgattcgtaa
    26281 cacgtggtaa cacctacgta acaggtaccc gttaccactc aatcccaacg gggacaaggg
    26341 ttctagcgtt ttggtaacag gtaacgggta aaatccagta acattattcc ccagcatatt
    26401 agctaactat ttcaaaagtt ttaaggaaat aaggtgttac cccgttacca aattgctgta
    26461 tcccttgggg cagtaagggt aaactagtaa cgggctaggt gttactgacc cgttactacc
    26521 cgttactttt tgtactttcc agctcgattt ttattttcat aggcatttga cttattgtaa
    26581 aatcctcgtc gtttatttct ttttggcagt tgcttccgtt cccaaccagg aatactgcta
    26641 ataatcaagg caatcttttt agtttctgca tctgatcgac cattcaataa atcctttgct
    26701 tgtttatcaa aaacagcttc caaaatatca gcggtgagta cgctatcaac taaataaaac
    26761 tcttgttgca tagtagctcc attatagaaa tgctgtaaat ttttctcatt acatttactg
    26821 tcaggatagt tctgataata agaacgtctc gcccacgctg gagcttcttc ccatttatac
    26881 gggatttcca tttccaaata tttagtgatt gcttctttaa ttggattctc catcttggca
    26941 tcctcttgat agtcttttgc aatttctaag acttctttat cttcatcggg actaaaatag
    27001 attcgttgtc ctttatcaaa aagcaccttt gcttcagcta acacttgaag aaaatattca
    27061 tcctttgttt taaacggatt catctttggc ttattcttac aaggcaaagg ataaaaacgg
    27121 cggtttcctg tatcatcttt caaatactgc ccatcattcg acgttccaat aaatacacat
    27181 tgccttgccc aagctatgac attccgacca taaggaggtc taattttatc gatcttggca
    27241 ctgatgaagt tttttatctg ttcaatttta gttttgttca ttgaagataa ttctccaagt
    27301 tcaataatta cattgccttg aagctgcata taatcatctt tcttttcacc tagcgaatct
    27361 aatgaatcgg tgaaaaaatc atccgtgtaa agtaatgctg gaacggtact cttaccaatc
    27421 ccttgcggtc catccaaaac tggaacgatc tcaaacttta ttcctggttt atagatacgt
    27481 gcgactgatc cgactatcca acgttttgtt acttctcgtg tgtaaaggtt gtcgtctacc
    27541 cctaaaaggt caataaacag tgtttccagt ctaggctgtt gatcccattt aactttttcg
    27601 atacgttctt ttactgggtt gaaaacattc tttctagcca catttactac tgctttgtgt
    27661 attttgttgt catcaaaaac tacattgtag ttttcttcaa tatatgccgt taaggcatcc
    27721 tcaaaactgt cttcccattc tccagcggta cgattgatcc aaggagaatc ttttaatagg
    27781 tgaatgtatc cgctaaattc gttataagca attgttccag ctaaattgct atcattggtt
    27841 aaaatcaatt ctgcattcac aacactgtta cttttaatct gatcgtcttt attgcatctt
    27901 aatcgtgaca tccaggtttt caattcaatg atattgtctt cacgctcgct ggcttcggga
    27961 aagtctaaaa tactatctaa tttatgctta tgagccatga gctttccccc tcaaatcttt
    28021 tttaatcatc gatcgaataa tttgattcac ttctttatct tttagcggtg gttcattgta
    28081 cgcatttgct gtataaatca attctgctaa agtttgatta tctacaccgt gccatagtaa
    28141 ccaaccgcat aacttagtta aatgatcgtt tctactacct tcgccagcgc cttgaacaat
    28201 catatctaat ttttttgctg tccctgtttt tgctacgggt cggtattgag tctttatttc
    28261 ttgcggtttt tttagcagta ggttcactag ccaatcggga gctggtgcaa tattatttag
    28321 agatacgttt tcaataggtt catatgctct cccaacactt cttgaaataa tcatagttgg
    28381 cgctattgga actcctttag tgataatatc aatcccgctt aaacttttac ctttatcatc
    28441 acgcccgaag atatcagcaa atgcacctgt ttcttgttgg ataattacac catcaggaat
    28501 tttaaagaag aaatggattc ctttacctgg tgtgatttcc gtataagtag aaggaaatgg
    28561 atcatacatt gaagcaagtt ttttataatt ctcaacgccg tttcgtgttt cagaatgttg
    28621 atctaggtca actaccacca tttgatgatc ttcaagcatt aacccaatat ttctagttgc
    28681 atcttccgtc caccattcat taatttgatt agaatcgtta gttgcatcat tccaaccact
    28741 agagcctttt attggaattt tagttccagg agataaggga taaattgacc atcccgactt
    28801 ttgtaattcc gttgctgctt gataattatt tgccaataaa cctctcctcc tttttcaaaa
    28861 tactggtata aatcattcag acacctccat gagcttcaac tgctcaagtt cgtatttcaa
    28921 cccatcccga acaaattcag caccttttaa ttgattctga tactttaccc atgtattaaa
    28981 tgttttcacc cttgcttctt gcatatcagt aatgtttaac catgcggtta aaatctgatt
    29041 ttttagctcg ttgatttctc gacaaattat tttgtgttct gcatgttttt tgagtacata
    29101 caaaactgtt agtttttcat tgaatgaatt tccaaagtag gttttcccga tattattttc
    29161 tagttcagcg atcccttgat taatttcctc taattcaagg ctatcgagtt ggtttaatag
    29221 ttttgttttg tacatctact tatgccctcc agttcttaat tgatagtcgt actcgtcata
    29281 agcagtgaag agatataacg ctaatcctgc aaagatagct atttttgcaa ttaatggtaa
    29341 taagccgaaa attccgccga ttaaaatgct tcctataatt aagtttcgtt tcataatggt
    29401 tccctccgat tttttaatat ggtataatcg gaacaaagaa tatcgggttt tatcccaatt
    29461 aagttgcctt tatcctgtcc gccaagactg ttatggataa ggctttttct ttgttctcga
    29521 tcatgttaat gctcctaagt tgtctgcaat tcagcagaac gattataaat tttctcactc
    29581 actgaaaact gcaacccata tttttcttta acagtcatta attctactgt ctcatctaaa
    29641 ataggttcac gatcaaccaa catttcagga gtcatatctt tcttttttac cattttggaa
    29701 tgaccataaa tagttgatac agctttattg gctatcacat tcgctttaat gtagtctact
    29761 ggttttggtt cagtaatagc gtggcttaat ttagtcattg cttctttttg gtgttctttg
    29821 tctagcatgc ggaatacttg aaagccttca agtcctgtgg actggcgtag ttctttgatg
    29881 atgtcgaaaa tccagtcttg aaatgcctcg gcttcttttt tacggctttt gaatgccatg
    29941 cggtaaatat tttttttcgt tgatgatgtt tacatcttgt gttctgccca atgaatcgat
    30001 gaccttacta gtagtaaccc catctttagg caaagagtga atggctctag taacctgttt
    30061 aagacctaac gcttccgcaa catctttcgc cactgcccac cattcatcat taatatcgac
    30121 aaatcttata atgtgtccgt tccaaatttc agttttcatt caacgtcctc cagttcaagc
    30181 cctagcaagc tcgctaacaa ctcaacttgc tgatacaaaa tttcttgata ctcttcaata
    30241 gaaagtcgtt ctttaaactc tgcttgtact aaatcgttca agccacgcaa tactccgctt
    30301 accgttggtt cttgtggtag caatttttga tagtccattt gttcgtacaa acattcaaac
    30361 gtttcttcta gcgctttgcc ttccaagttt gctaattctg ccgctgtgtc ttctgtaaga
    30421 attgttgata atactttttc gattgcttta ttttgtgtcg attccattat tatttcctcc
    30481 tattaatccc tttgccccca cggcttgcgt ttatttttgg tattcttcca taaagcgatc
    30541 acattcatct tgtgaaattc ttacgactcc atcaactata atcaacttta aaccgttagg
    30601 aacatatttc tgcatcatag tattgtatga aacgttcata tacttagcag cttgtgcctt
    30661 gttcatatat tttggaaact ggtacttttt ttctaataat gaaagtaacg ttccttgagt
    30721 ttgctctact tgttgaacaa ctcgatcgtc aataatttta aaagctggat catcaagcgt
    30781 gagtttgtca atttttaatt ccatatttat cccccctcgt tagcatacat ttctatacaa
    30841 agattttaaa aaaatgcaag aggctcttga atacatttct atacctctta taaactcaat
    30901 ataccatcat accttttcat ttgcaagaat tttaacatac ttttttgaac ttttatgttt
    30961 cacacatatt taaatcgtga tatagtagag aaaatatatg aatggtggtg aaaatgtggc
    31021 tagaaaaaaa taccccgaag cagtggagac aggacaaaga attagactgg ctagaattga
    31081 aaagggactc tcaatggaac aacttggagg taaattatcc ccaccggcca gtaaaggtgc
    31141 cgttagtaat tgggaaaatg gctataatct tcccaataac accaggctta aacaactttc
    31201 aaaggtatta gatgtatcca ctacctatct attaaacgga tcttatacat taagagatat
    31261 gcatatgatg cccgaaatta agcgaaatgt gcttgaaaag caattgaaaa aaactattga
    31321 aaaaaataac tctataattg attctaatct ttctaatgct atcaataaca tcgaagaaga
    31381 taacctgact gtctcacaga agatttattt aacaaacgca ataaaatatc ttaaaacagg
    31441 atctcgaatt gaatttcctg acgggagtac cccaattgct tatctcgcag ctcttctaaa
    31501 tacgctttta cgatatgact cattaattaa tgattcagaa aaaaatacag atgagaaaaa
    31561 agagtatcaa gaattttatg aaaatgatct tctaaaagag ttcacagctc ttgttactga
    31621 gctaaataaa cactattcaa aataataata aactgcctcc tccaaccgcc cccacggctt
    31681 gctacactat ggagggaaaa ctattatggc aacattcaag caatatgaaa ctaaaaaagg
    31741 taaattttgg ctctatgaag cctatcttgg aatgaacaaa atgactggca aacctgataa
    31801 ggttcgtaga cgaggttaca aaacaaaaaa agaagcccag cttgccttgt cacgcttgca
    31861 agtggactac gataaaaacg gtattaaaaa agcgaacaac gaaacgttta aagaagttta
    31921 tgacttgtgg ctagaaacat ataaaaccac tgtccgagaa gccaccttca tgaaaactga
    31981 aatcaagttt agaaagtgga ttcttccaaa atatggaaat cttcgtgtga atgaagtcac
    32041 ggtaaaaaaa gcccaacaaa ttgtaaatag ctgggctgaa actactgacc aatacaaggt
    32101 acttcattca acttctaagc gtatttttag ctacgctatc aatcttggga ttattgagac
    32161 taatccacta gacaacgtat taatgcctaa aagaaccacc actaagcata aagaaaaggt
    32221 caaagtttac agtaaagaac aactgatcac cttgtttgat tacctaaaca gcaaacctga
    32281 aacttatcga aacgactttg ataaaacgtt actacgcttt ctcttttata gcggggttag
    32341 gatcagtgaa gcccttgcat tgaattggtc agacattgat tttcaagaaa atacggtcac
    32401 catcaataaa acactaagcc aatccaaaaa tggctataaa atcagtgatc ctaaaactga
    32461 ttgtagtgct ggggttcttc cactagatga aagaacaatt tctatattaa aaaaatggca
    32521 gattaatcag cggaaatata tgctgacact tggtgtaact gatccgacta tgattttttg
    32581 tggtatctat aaagaactaa tcacacacca cgctatttat gcaagattga ttactatttg
    32641 tgaaaaagct atggttccat ttctcggtgt gcatgtagca agacacactc atgcttcaat
    32701 gctcttggac gctggagcta ctatggtaga agttcaaact agattacggc atacaaaaat
    32761 tagtcagacg atcgattgtt acggtcactt ggcaaaagaa acgaaagaaa agactgttga
    32821 aaaattagta caacacttaa atattgacta gatggcatac aatatggcat acagtaaaaa
    32881 ataaaaaaag gagaaaccct atcatatcaa ggtttctcct agctttatag tctattcttc
    32941 ttcttccgcc atgaagttaa gttcattgac gataattgaa gatagttgat aattaaatga
    33001 tttctgaata gagctaatca ctttattgat gataattcat cttgactggt tttctaatcg
    33061 tattcaaaaa cgtattcaag ttgcgtggtg tgcttttgat agaaaagact gaagaatctg
    33121 tcaaaataaa atatgttacc aactgatcct tccaatggct cttttgggaa tcaagggaaa
    33181 ggtatatgct ttggatatat aattcatttg acaaattacc atagactgac agtgtatatt
    33241 atcaggtact atcatcgata acgtagccaa tatcgtaccc aaaatagcag gtagagaagt
    33301 tatttgagga attaaattat tccatttttt ctataaaaat aaatatagtt aatta
//
LOCUS       DAZKNO010000008        31244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000008 DAZKNO010000000
VERSION     DAZKNO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.008"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..1602)
                     /locus_tag="ACG8MY_000273"
     CDS             complement(34..1602)
                     /locus_tag="ACG8MY_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0429506.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1606..2016)
                     /locus_tag="ACG8MY_000274"
     CDS             complement(1606..2016)
                     /locus_tag="ACG8MY_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0429507.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(2013..2936)
                     /locus_tag="ACG8MY_000275"
     CDS             complement(2013..2936)
                     /locus_tag="ACG8MY_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0429508.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2933..3811)
                     /locus_tag="ACG8MY_000276"
     CDS             complement(2933..3811)
                     /locus_tag="ACG8MY_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0429509.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3798..5135)
                     /locus_tag="ACG8MY_000277"
     CDS             complement(3798..5135)
                     /locus_tag="ACG8MY_000277"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0429510.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5138..6241)
                     /locus_tag="ACG8MY_000278"
     CDS             complement(5138..6241)
                     /locus_tag="ACG8MY_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0429511.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6315..7823)
                     /locus_tag="ACG8MY_000279"
     CDS             complement(6315..7823)
                     /locus_tag="ACG8MY_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0429512.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7789..8481)
                     /locus_tag="ACG8MY_000280"
     CDS             complement(7789..8481)
                     /locus_tag="ACG8MY_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0429513.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8503..9477)
                     /locus_tag="ACG8MY_000281"
     CDS             complement(8503..9477)
                     /locus_tag="ACG8MY_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429514.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9777..10649
                     /locus_tag="ACG8MY_000282"
     CDS             9777..10649
                     /locus_tag="ACG8MY_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0429515.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11107..13305)
                     /locus_tag="ACG8MY_000283"
     CDS             complement(11107..13305)
                     /locus_tag="ACG8MY_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0429516.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13537..14529
                     /locus_tag="ACG8MY_000284"
     CDS             13537..14529
                     /locus_tag="ACG8MY_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0429517.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14589..16010
                     /locus_tag="ACG8MY_000285"
     CDS             14589..16010
                     /locus_tag="ACG8MY_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0429518.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16003..17502
                     /locus_tag="ACG8MY_000286"
     CDS             16003..17502
                     /locus_tag="ACG8MY_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0429519.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17541..18935
                     /gene="uxaC"
                     /locus_tag="ACG8MY_000287"
     CDS             17541..18935
                     /gene="uxaC"
                     /locus_tag="ACG8MY_000287"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0429520.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            19094..19777
                     /locus_tag="ACG8MY_000288"
     CDS             19094..19777
                     /locus_tag="ACG8MY_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0429521.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            19779..20615
                     /locus_tag="ACG8MY_000289"
     CDS             19779..20615
                     /locus_tag="ACG8MY_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0429522.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            20626..22380
                     /locus_tag="ACG8MY_000290"
     CDS             20626..22380
                     /locus_tag="ACG8MY_000290"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429523.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(22656..22997)
                     /locus_tag="ACG8MY_000291"
     CDS             complement(22656..22997)
                     /locus_tag="ACG8MY_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0429524.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(23065..25236)
                     /locus_tag="ACG8MY_000292"
     CDS             complement(23065..25236)
                     /locus_tag="ACG8MY_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0429525.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            25447..26304
                     /locus_tag="ACG8MY_000293"
     CDS             25447..26304
                     /locus_tag="ACG8MY_000293"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0429526.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(26375..27232)
                     /locus_tag="ACG8MY_000294"
     CDS             complement(26375..27232)
                     /locus_tag="ACG8MY_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0429527.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(27217..29919)
                     /locus_tag="ACG8MY_000295"
     CDS             complement(27217..29919)
                     /locus_tag="ACG8MY_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0429528.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(29932..31221)
                     /locus_tag="ACG8MY_000296"
     CDS             complement(29932..31221)
                     /locus_tag="ACG8MY_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0429529.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
BASE COUNT         9100 a         6478 c         5586 g        10080 t
ORIGIN      
        1 aagttagtga acctcattta ttaaagaatt aaattacaga tgatcttttc catcatactt
       61 gaaaaactca aaatcagcat aacttcctct ttttttattt agatcatgca ctgctatccc
      121 gataaaattt ccagtaaacc ctcctgagag tatacacatg tttttttgcg gacctacttt
      181 cttccattct tcagttgata cgtctttcaa atgaaactgt gctgtctctt tctttacagt
      241 gactttcaaa aaagtcttct tttctgtgag tggaatcgtc caatcttcta tttgtgctac
      301 tccgttttct gattctatca tacgtaggca ttttcccttg tcctcattcc aagtaacata
      361 acaataaata taattggatt catttaagta cagtaccagt ccagccatct gattaaaatt
      421 attaggcatg tattcaaatg ctgtctccgc atcaaaagta aaatccgttt gccgaatagc
      481 taacaaatga tgttcgaagg tagattgaag agattcaccg gaaatcagac gtaaaaaacc
      541 tggtctacat tttgtatcac accaatcagc atttggcaaa atgcgtctgg cattccagtt
      601 cttctttagc tctccttcaa atgtatccat aaactgatga tctttttctt gttgtacctc
      661 ttcatttgtc tcaatcatta tttcttccat cggagttgtc tggctatctt taagtcttaa
      721 ccaatcgtcg gacgtccaat acacttcttg aatagcggtt tctcttccca aaatagccgc
      781 accgtttaat ggtctggtac aaagatatgt catgtaccat cttcctgacg gggtttgtac
      841 taagctccca tgtcctgtac attgcaatgt agaagcaggt ttgtcactag ctgtcagcat
      901 aggaaaacca ggatcaagct cgtaaggacc agtaatattc tttgctcggc aaactgtaac
      961 tgaatgtcct cttcctgtac cgccttctgc cgtcatcaaa taataatacc cgttacgaaa
     1021 atacaaatgt ggtgcctccg ttttcgctaa ttctgtacct gcaaatattt tatagatggg
     1081 accatcaagc ttcttcttct ctgcatcata ttcttgtaat acgataccgc tggatttatt
     1141 cggtgtgtcc agacgataat cccatagttc attgacaagc catttttttc ccgtttcaag
     1201 atcatggaac aaagaaggat caaagccgtt actatttaaa taaattggtt cggaccaagg
     1261 acctgtgatc tcagatgcag tgatcaaata attgtggaca tctttaaacg gacggcctgc
     1321 acttttgaca tctgtgtaaa caagataaaa caatccatca gcataactga tctgtggcgc
     1381 ccatatactt ccatttgtag gatttccttg caaagatact tgagtggtca aaatatccgt
     1441 tgtatgtgac cagttgacta aatttttcga ctcatatact cggattcctg gaagccattc
     1501 aaaagaggag acaataatgt aataccgctc tttcactctt aatatattag gatctggatt
     1561 gaatcctctt aaaataggat ttttcacttt gatagtctgc atgattcagg ctccttttct
     1621 cattcttact cgatataaac tgaatgcccc agctgcgaaa taaaaaaatg caaaaggaaa
     1681 cgcaataaac tgagtgccaa tcaacaatat gaatcctgtt ataaaataca aaccagtggc
     1741 agttgttaag tattttcctt tagcagtcaa aaacaatgct actgctaaaa aagcaagtga
     1801 aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa atgtattttc
     1861 taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa actctgacgc
     1921 agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata gactgccacc
     1981 gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg cttttctaaa
     2041 tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc agagaatttg
     2101 acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg aacatgccca
     2161 cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct tcctaaatag
     2221 acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata ccctgttttt
     2281 tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc taagctatgg
     2341 atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac cgttccttca
     2401 atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc cggtgtacaa
     2461 aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt atcctgataa
     2521 ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa agcaacagcc
     2581 tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc taatacgaca
     2641 ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct accgtcaaga
     2701 tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc cacaagttta
     2761 aaattggata atctgctttc tatatattca tcatatcttc cttcttttac tatcaagacc
     2821 tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat agtttcaaaa
     2881 tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat cctcataaat
     2941 gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata tgaccgcctg
     3001 ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt ttgtccagcc
     3061 cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt ccaactaagc
     3121 taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa gctgtcaata
     3181 gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc aaaagtattt
     3241 cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc aatgctgtca
     3301 cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg
     3361 tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata cgcattcccc
     3421 ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa atacctccgc
     3481 gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct gtcagtactt
     3541 ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt ttcccgactg
     3601 aaaaatctcc gccgtgagaa tgcaggtaaa ggaccggcgg aaacggtcct tctttttctg
     3661 gataagcata gagggctggt actgtttcca attcatttaa gtacaattga tagtattcaa
     3721 gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta tgtctgctag
     3781 atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac aaccagttca
     3841 tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt agtattttct
     3901 aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga aaccttaccc
     3961 gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat catagcagga
     4021 acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt gatttcttgt
     4081 atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt ccttgccgtg
     4141 atattcgaaa aatggatcct tctgaatgct ggtgtccgtt catctatcgg ataagccttt
     4201 ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag attcagtata
     4261 aatggacaca tcacattatc catgactata ttggatactc gaatatcttc aacgatcccg
     4321 cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa gatacaattt
     4381 gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac accgccatgt
     4441 ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta
     4501 tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg gcaattacta
     4561 atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga atccgctggg
     4621 ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa
     4681 cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt atggaaactc
     4741 atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt atgccaccat
     4801 ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc tacattttga
     4861 gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga atgaacaaca
     4921 ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa atgaagttcg
     4981 acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac aactacggtc
     5041 ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt attcaattca
     5101 tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc acttcaatca
     5161 atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt tcactgatgt
     5221 agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact tgacagtttt
     5281 tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt aatccttgat
     5341 gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa cgtattcctt
     5401 tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt cctttccgtt
     5461 ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat ggaagtaatg
     5521 cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct tcatgaagta
     5581 attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc caaaaatgat
     5641 gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc catgcatgaa
     5701 tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac tgtctcaaaa
     5761 catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa aaaggagaac
     5821 ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc caaaaagctc
     5881 cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct gcttttttat
     5941 atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta tttacatggt
     6001 cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc acgaaatagt
     6061 cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt
     6121 ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac cacttttctt
     6181 catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac cagttttcca
     6241 ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag aagtatttgt
     6301 cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt
     6361 cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc ttcgtcaagc
     6421 tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc tgatcctact
     6481 tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa ttcttcattt
     6541 tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa agagaacgtc
     6601 acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag atccatatcg
     6661 ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat accgccagtc
     6721 atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat
     6781 tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat accatttcct
     6841 tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac aggcactaat
     6901 tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc caccattcca
     6961 gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact
     7021 gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt caaagctttc
     7081 atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac agcccagcca
     7141 tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat atcactaggc
     7201 gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc gttttctgta
     7261 aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag tcctagatta
     7321 tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg tatcggtacg
     7381 ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat ttgacttaaa
     7441 tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt taacgcattg
     7501 cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc tagttcatca
     7561 gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca gttgaatgtc
     7621 agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca
     7681 tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc tgaagaactg
     7741 ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact aagacctaat
     7801 tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa tatttttgtt
     7861 ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata tagaatttgc
     7921 tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa cacctggcat
     7981 tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta atgagtaatg
     8041 aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc ctttccaatc
     8101 ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga acgcagcaat
     8161 atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa gtaaaacaat
     8221 cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt ctttaaaata
     8281 tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac
     8341 tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa
     8401 accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat
     8461 tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc
     8521 tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt
     8581 aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat
     8641 ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg
     8701 ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg
     8761 acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc
     8821 tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga tgatcgtaga
     8881 ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga
     8941 tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc
     9001 gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg
     9061 gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag
     9121 taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga acaagatcaa
     9181 cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa
     9241 accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac
     9301 gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat
     9361 cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt
     9421 ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag
     9481 cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa
     9541 ttacgtatat aatcattttt accgtaagta aaaacgctta catagcaatg atttttgtgc
     9601 tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt
     9661 tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc accgatgata
     9721 aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga
     9781 aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt
     9841 tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta
     9901 cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt
     9961 atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta
    10021 caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca
    10081 gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca
    10141 gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc aattcttact
    10201 gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt
    10261 tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg
    10321 gtgtattttt caaaattatg ctgccattag cgttaccgtc tattgctact atctcgcttt
    10381 tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat
    10441 ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag
    10501 cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg
    10561 ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag
    10621 gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt
    10681 cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt
    10741 gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg
    10801 tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa
    10861 cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg
    10921 ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa
    10981 gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca
    11041 aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct
    11101 cttgattcac aaatattttt ttcgatattc tcccggcgta atcgattcct tctttttaaa
    11161 gaaacgaata aaattttgcg gatttcgata ttgcaatctt tctgcaatct cttttacgga
    11221 gaggttagtc tctttcaacc atttttttgc tacctctaaa cgatgatttt gaacaaaatc
    11281 accaaatttt tctcctgttt cctttttaaa tacattactt aaatagttag ggttataatg
    11341 caacctatca gcgatcacat ctaaagacag atcacgatca tattctgtgt agacgatatg
    11401 cataatattc tccgacaaat ttttaaattc atgatcagtg attgcttgag agcattctgt
    11461 gattggcaga actaattgtt ctaaaacaag tttttctagt tctttgggat gataattgtt
    11521 cagtgctttt acataaattt gtttcatgtt atcaaagttt tttgaatcta ctcccaataa
    11581 ctgtcctaat tgaacgagtt cattgatcaa gcggaccgaa actacttccc gactcaaagg
    11641 atttttattt tgtgcaaaca atgcatcaat caattcatgt actaaattct tcacttcctg
    11701 atttccagaa cgaatggctt caaacaatcg attttgttct tctatcggat atctaataag
    11761 tgttttttca tgagcagcag gaacgatttc ctgataaaaa ataattgaac ttggaccagt
    11821 atttacacga taatacaggg cttcttttgc ccgatcaacc gattgcttgc tttcattcag
    11881 cgtttcgaac cggttactga tgcctatact gatagttaaa ttcaagtatt cttttacacg
    11941 tttttgaatg agtcggcaat actccatgac ttttttgctt gcttctgcat catctttatc
    12001 cattcgataa atagtggctt gcatttcttc attcaaaacg atcggcagca ttcgattttc
    12061 ctttggcaca atttccgtga tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc
    12121 accgtgacta tcttctaaca gatcgatttg aaccaaagct gtataatagc agtcattttg
    12181 agaactataa ccaaattgct gcaaccgctg atctagttct cgcctttcta ctcgatttct
    12241 aaataaacta agtacaaata gcgtttccag ctgtggcttt tgggtaacta agtgtgcact
    12301 taacgcttct ttttctccta tgatccgact gactgattga gcgaccaaat ctagttcttt
    12361 cccactgaat ccttttgatt taagattcag acgttcctgt atttgagaaa tcggacgggc
    12421 aaatctgtcc gaaaataagt aggaaagaag accaatgagt cctaccagtg ttaaagacac
    12481 tgcaactagc ccgattctca gattgcggat cattgcgcca attgcactag agtcaacttc
    12541 taatctgtaa atccaattat tataatctga tttcagtact atatacgttt tatctttttc
    12601 tcttatcact ttcagctgat tgtaatctag atccacgtcc gttaatacag ttggttgctc
    12661 tttatttttt actgtctgag tagtaaaaag gttgccaact tcgttttcaa tagaaagtat
    12721 gtcttctcca ctttcaccaa caacttgttc tattgtatgt ttctctatcg tagcaattcc
    12781 caatgcatat ttttcttttt caaacattgg caaagaaatc accatttcga ttcctttgtt
    12841 tttcttttgc caaaataaat tatttttgtt atttatgtac ttctgttgat acttttcaat
    12901 ttcctcttcg gtcagttgac tcaacgatcc attgattact ccccatttac cagccaaact
    12961 gactaaatca taattattat catccataac aatcgtttct ataaaattta gttctttcct
    13021 gatttcagaa taagtggagt aatctgtata tgtcagcgga tgcttgaata tgtccgagaa
    13081 atttttggtc gaaccatacg tagaaaacga atattcaatc gttctaattt tccattcaac
    13141 gttagtttta acttgcctga gcaaattctt tttgtctgtt aaataactcg attcgatcga
    13201 ttcataggta ttctggtaaa tgaataaact aaatcctatg acaagtacgg ctcctaataa
    13261 aaacatcggt aaaaatattt tataaaatac tgtacttttc ttcattttct tcctctcctc
    13321 cttttaaaat aaaaggcttt catttttctg taaaattgat actcatctaa ttaaatgagc
    13381 attccccgct ttcaagaata gcataattaa aaataaaaat ccaagtaatt tttaacgtgt
    13441 gcaatctttt catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc
    13501 gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatgtcgct
    13561 aaagcagctg gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata
    13621 aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac
    13681 tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt
    13741 ttgcataaag gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta
    13801 ttagacacaa attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc
    13861 aacctgcaac gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc
    13921 atcgactatc tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat
    13981 ccagcggtta tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac
    14041 ggtattggat gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg
    14101 acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat
    14161 ccagcctatt ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac
    14221 ctattatcac tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt
    14281 ggtgcattga gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc
    14341 gggttcgatg attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag
    14401 cctttgaaag aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg
    14461 acgatcgaac aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat
    14521 tacttgtaaa ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg
    14581 aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag
    14641 ttcttcagtt tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc
    14701 tgaataacca acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc
    14761 ttggaaatat gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca
    14821 atggcgaaat cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt
    14881 atgatgaatg ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct
    14941 ccaatacaac agaggcagga atccaattcg tggagactga taatttaaca gatactcccc
    15001 cacagagcct cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag
    15061 ctggctttac catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa
    15121 tcattttacg ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa
    15181 aagaaaatgt tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag
    15241 atgcaggtgt tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac
    15301 cattcatgct ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac
    15361 aggctggctt gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc
    15421 atttgcttaa tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa
    15481 ctgtagaaga agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc
    15541 tagaattgat tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa
    15601 tcaaagaacg tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata
    15661 gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa
    15721 atcaggtacc accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg
    15781 atcaagtaaa gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg
    15841 ataatggaac agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt
    15901 ctcaaatacc gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag
    15961 gtgtccggtc agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa
    16021 ccaacaatga aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa
    16081 caaacacaac tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt
    16141 cataagattg cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca
    16201 atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa
    16261 accaatcttt ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac
    16321 ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt
    16381 aatgatttgt ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc
    16441 aaacagttca aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag
    16501 catccttatg gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg
    16561 gacgcagtaa atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac
    16621 aatacagttc ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt
    16681 ctagtggctc aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt
    16741 ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt
    16801 ttgaaatgcg gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca
    16861 ttttctgact acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg
    16921 tttggcgcag aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc
    16981 gttgacttga tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa
    17041 aacccatctc ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc
    17101 tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc
    17161 agaaaaccag ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgct
    17221 cttgcttcat ccgattgcca gttggtcttg tttaccacag gaagagggac tccgtttggc
    17281 tcatatgtcc caacggtcaa ggtatctacg aatacaactc tttttgaacg caaaggtcat
    17341 tggatggatt ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat cttagaatcg
    17401 tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac
    17461 gttcgagaaa ttgccatttt taaaaacgga gtaacacttt gattttcata aaacagtata
    17521 aaaagaaaga aggaagattt atgtttttat ctgatgattt tttattaaaa gacgattggg
    17581 cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat tattgattat cactgtcact
    17641 tagatccaaa agaaatttac gaaaacaaca atttcagcaa tctgacagaa gcttggttag
    17701 gaggcgatca ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca
    17761 caggaaatgc ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta
    17821 tcggaaatcc gctttacagt tggtcacatc ttgaattgaa gcgtttcttc ggtattgact
    17881 tgttgataaa tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa attttggcag
    17941 ctcctgattt tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa
    18001 cagatgatcc cgcagacgac ttgcactatc atcagttgct tgctcatgaa gaaccagatt
    18061 tgaaggttct accttcattc cgtccagata aagcactcaa tattgaaaaa gaaggattta
    18121 atgcatgggt cacactactg gaagaagcag ctgaccaaac gatcaccacc tatcaagaac
    18181 tgatagaagt cttagggcag cggatagaat acttccatca acatggatgt cgtgtttccg
    18241 accacgcttt agacatctta cgctataaag aagcggatga aacagtatta gaagagattt
    18301 ttcaaaaagc acgagctaac aaactattat cttcagatga aatcgatgct tatcgtacag
    18361 aaacactgat acatttgacc cacttctatc attctcataa ttggacgatg cagctgcaca
    18421 tccatgctta tcgtaactgt aatacacaaa tgttggaaaa attagggccc gataccggct
    18481 atgatggaat gaatgatctt tctttgacta ttcctctgca aaaattattg aaccgtgcag
    18541 aagaaacgga tcagttgcca aagacgatcc tttattcatt gaatccgaat gactatcctg
    18601 cgattttagc tttgatgggc tgtttccaaa aagaacagaa tggaaaactg cagttaggtt
    18661 ctggatggtg gtacaacgat actcgagctg gtatgcgcga tcaattgaca caatttgctg
    18721 atggtagtgc tttggggaat tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct
    18781 acactagaca tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc
    18841 gaggagaagc acctgaagat gaacaacttt taggaagtct agttcaagcg attagttata
    18901 caaatgctaa aaattacttt ggcttttttg cctaattgat taatagtaac agtaacgaat
    18961 caaaaacagg atagtgaaat aacggaatcc gtttatccac gttttagaga agaagacgga
    19021 ccaaatttgc tccgtcttct tctcttgctg ttctgcttca ttgcctataa acctttatga
    19081 aagaagagat aacatgccta ctatccatat tgccggtgat tctactgcgg cagaaaaaga
    19141 tgaaaacaag cgtcccgaga caggttgggg agaaaaaatc tctgcttatt tttcacccga
    19201 gatccagatc aataaccaag caaagaatgg acgcagcacc aaatcattta tccaggaagg
    19261 aaggttagcc cgtctagctt cacagtttca gccgaatgat ttcttatttg tacaatttgg
    19321 acataacgac cagaaaatcc aagaagaaag aggtacgctt cccttcggta gctatttaaa
    19381 aaatctgcag atattttttg aatgcgcaaa aaaagcgaac gtccaaatgg tcttactgac
    19441 atctgtgacg cgacgagatt acttagaaaa cggaacattg aatccagata tacttggaga
    19501 ctatccaaaa gcgatgcgtg catttgctga gaaacaccat attcctcttt tagatgtgtt
    19561 ctcaagatca caagaattgt tccagacctt ctcaaaagaa gagacaaaaa aattctacct
    19621 gcatcttata cctgatactt gtaaaaatta cccagaagga ctaaaagaca atacccactt
    19681 tagtccagaa ggcgctgata aagttgcccg aataattgtt gaattgataa aagaatcccg
    19741 tctgcctctt gctaattatt tacaaaaagg agtttgagat gtttccatta aatttaggca
    19801 tccgtgccca cgatttagat gttcgaaatc gtgaagaaat cgttcaaaaa gtatcacagc
    19861 ttcacttgtc ccatatccaa tttgcaccac aaaaagcatt tcctgaacaa cttaaattaa
    19921 ctgacatgac acttgggagt gctgcctatt ttggtgaata cttccaaaaa aacaatatcg
    19981 aactttccat tttaggatgc tatatcaacc tatccagcaa aaatcaagaa gttcgtgcac
    20041 aagcattggc aactttcaaa cagcaaattg ctctgtgctc tgcctatcaa gccaagatga
    20101 ttgctacgga aaccggtagt gtctctgtcg gttacacaaa agaaaatttt actgaagaag
    20161 cttatcagat cgccagaaat tctatcattg aattagtcgc tcatgcagaa aattttggcg
    20221 taactgttgc tgttgaagca gggatcaatc atccaatcta taactatcag ttagctaaac
    20281 gtttaatcga tgaagtagct tcaccaaatt taaaaatcat tttagattgt gcgaatttga
    20341 tgcataaaga aaatcatgct gatcaggaaa aagtgatccg aggtgccttg gacaatttac
    20401 atggttatat tacagcgctt catctcaaag actatatcat ggaaaatgga aagatccgca
    20461 tggtacctgt tggcaagggc tgtttagatt tccgcccggt actgcattat atcaaatatg
    20521 agcgtcctct aatgtacgcc actcttgaag ctactcctga gatttatgtt cctgaagcaa
    20581 tagaacactt gcagtttatt taccaagatt gttaaggaga agattatgga ggaacgaata
    20641 cagaaaagtt atcaaaaata tcatgcaaat gcttttcaga cattgctgca tctttatcag
    20701 ggaaattatt ggcgttttgc catgtcagga ctctttttta ttatcaaaca tcttccatct
    20761 tggttaatgc cgatcgctat tgccaatgta atcaatgcag cttcatcgaa agattcttct
    20821 cagttgcagg ccatttattt caacgctttt ttgatggctt gtctgattgg tcaaaatata
    20881 ttgacgaatt attttcatgt aaaataccat agcacttcga tccgtcaagt agaagctgga
    20941 ctaagagcga cccttatcaa gaaaatccaa gaactatcta ttccttatca aaaagaattg
    21001 caatctggac gaatccagtc aaagatcatc cgtgatgtag aagctatcca aacgttgtcc
    21061 tctcaagtat ttgtcagcgg attaaatatc gctgtcaatt tattcgtagc tttaggtatc
    21121 acgctttata aaagtcctgt tgttttcatg tttttcttat tgactgttcc tctcgccgca
    21181 ttcgttactc tctttttccg taaaaggatc agacagtcca actcagactt caggcaagaa
    21241 atggaagaga cttctgcaaa agtgatcgaa gtgatcgaat tagcaccgat tgctcgagct
    21301 catgggttag aaaaacacga aacaactcgc ttaaataagc attttactca aatctatgaa
    21361 aaaggattcc acttagatat cctgcaatct gttttcggat cagtcagctg ggctagtttt
    21421 cagatatttc agctgatttg tttgattttc actgggatat tagctttaca agggcgcatc
    21481 caacccggcg atgtcgtgat gtatcagacg tatttcacga cgatcgtcaa ttccgtttct
    21541 ggaatgatca cgctgattcc tacgatttca aaaggcatgg aatctatcac ttctattggc
    21601 gaactgttga ttgctggcga tgtagaaact tatcacggta agaaaaaaat ccaacatttg
    21661 gatggaaatt ttcgttttga ggatgtttct tttctttatc cggactcact caaaccgatt
    21721 atcgatcatt tttctctaga ggtgaaagct ggagaaacaa ttgcttttgt tgggccatct
    21781 ggctctggaa aatctacact ccttaactta ttgattggat ttatccagcc gacttcagga
    21841 aaaatttttc tagatgatca gcctttggat gaactggata tgcgaagtgt gcgaaattat
    21901 ttagccgttg ttcctcaaac cactttgttg ttctccgctt cgatcaaaga gaacatcact
    21961 tatggcttga aaaacgtatc aaaagaacgt ttagatgaag tcatcgaagc tgcgcaactt
    22021 tcttcactga ttgaccagct tcctgatggt ctggatacct tagtaggcga acatggcaac
    22081 aagctgtctg gtggccaaaa gcaacgtatc tccattgcac gagcgttgat acgtcagcca
    22141 aaaatcattt tgctagatga agcaacctca gctttagata accagtcaga gaaaaaaatc
    22201 cagcaagccc tgaattacct gacagaaaca cccactactt ttattgtggc tcatcggctt
    22261 tcaacgatca aagaggcaga taaaatcgtt gtgatcgatg aaggaaaaat cgttgaaata
    22321 ggtacttacg aaaaactgtt agctcaaaaa ggctatttct accgcttgaa aaatgaatga
    22381 caaaaaatag gagctatgac aagacttttg tcgtagctcc tatttctgaa taaacggtgt
    22441 tctcctgcca actcctcggt cacaagtcac aatagtcatc aaacatgaga agctgttttt
    22501 tgaaattgtt ccttgtaact cagagttaaa cggtaggctc acgacctctt ggtaaatggt
    22561 gttcaagaga cagcctatcg gcaataagaa gcaattttgt catcgctcaa ggccgaacgt
    22621 ctctttcaca ccctctgttg tttcataaaa tagttttatt cgctgtaaag gtcgcgaact
    22681 ggtgtcatga tgatacggtt caagtcattg atgattgtac tgaagccttg ctctttttgc
    22741 atcaagtcat tgattacttc ttctgattgg attttcacag ccatttcttg tgctttcatt
    22801 gcatcttcgt cgctgaactc ttgtccactc atttgttttt cttgtaattc gatttgcatc
    22861 gcttggaagt ctttgaacag tttgtgtgct tcttcgtttg cttgtacttt ctcgtacgct
    22921 tctttcaacg ctttgaattc tggtaattca cggatctcac gttcgatttg attcgcgcta
    22981 tcgtagatgt ttgacatgtt atgccctctt ttcttttagt agttactctc tattgtaaca
    23041 tggggttatt cgttttcaaa ggggctactc tccttgatat ttccgccaga ggtctttagc
    23101 cgcttcacca attccctgaa cgccttcttt caccatatcg ccgaagtttt tcagcccttc
    23161 ttttgcttgt tcaccatatt gattcaagtt atcttgccaa tttccagact ctccattaga
    23221 atttcctgtg tttccaactt gatctgcagc taccacttgt cctcctgtgg cataggcatc
    23281 tgctacacta aatcctgttt ttgaagtata cggaaggatg ccttctgccg ctgatttgaa
    23341 gactttcccg acaacatttc cacttgtccc ttccaaatag tgcagcttgc tggattcttc
    23401 gtaacccatc catgtcgaaa tcactacgtc tggcgtgtaa ccgatcatcc attggtcatt
    23461 tgcttttgtg gcatcgaaat tagtttctgt ggttcctgtt ttccctgcta tcgtataacc
    23521 tgcaggattc gcagcagccg ctgttccatt tgaaaatgta ccaagaagca tgctggtcat
    23581 ttcatcggct gtttcctttg atatgacctg ttttggtttt ttgtttgtgt tatcgacgat
    23641 caccgctcca gtcgaatcaa cgattttcgt gataagatgc ggcgtataca tctgaccatc
    23701 attggcaaat gcactgtaag cacccgccat gatcaatgga gatactcctt tttccagtcc
    23761 accaagggct aagccataat acttatcaga ttttgctaaa ggcaggccga attcttctgc
    23821 tttatcatat cctttttgta aaccgatctc atgcaaaagc catacagtag gcaaattcaa
    23881 actttgtgca acagcttgat acatcggtac ttctccttgg tatgtcccgt caaaattatg
    23941 cgcatcgtaa taactttgcg gttcgtcttt caagatactg gaaggcttgt atccagcttc
    24001 cagtgctggt gtatatacgc ttaatggttt gatcgttgat cctggtgaac gacgcatctg
    24061 tgtagcaaag ctgaatcctc taaagacatg atctcctctt cgtcctacta gtgcttgtac
    24121 accacctgta ttcggatcta aagctactga agcagattgg accatggcac catctgaagc
    24181 atttgctggg aataatgcac tgttttgata agtagcttct aattgctttt gataatcctg
    24241 atctaaagct gtatagattt tatagccttt attcatgaca tcttcttcat caagaccata
    24301 ctcgttaact gcttcatcga tcactgcatc aaaataataa ggataacgat aaccagaatc
    24361 tgaattatcg tatgtgtcat taagatacgc gctgatatct gtctggcttt ctgcttgttc
    24421 ttgttctttt gtgatttttc cgttttcttc cattacactt agtactgtgt ttttccgatc
    24481 attcgcattt tttgggtgat cgatcggatt ataaatacca ggacctttca gcattcctgc
    24541 caaagtagca gcctcagaca gcgtaacatt cgttgcatct actccaaaat actttctgga
    24601 agcgtcttgg acaccccata ccccgttgcc aaaataggca ttgttaagat acatggtcaa
    24661 gatttcttct ttgctgtact tcttttcgat ctcgattgcc aaaaataatt ccttcgcttt
    24721 tcgatcaaac gtttggtcta atgtcagata cgcatttttg gcaagctgct gagtgatcgt
    24781 acttcctccg ccccctgatg ttccgccaga caagaccatt cggactgctg cacgggcaat
    24841 ccctttgata tcaaacccgt gatgctcgta aaaattccta tcttctgtcg agattactgc
    24901 atcttggata tagggagaaa tcgcgtttaa ttctacatat gttccttttt gggcgtacag
    24961 tgtgccagct tcttcgtttt tcttatcata aatgactgtc gattggctta atcctgattt
    25021 taacgtttcg acatttgctt gttttgctaa aataaagagg tagatacttg tcactaagac
    25081 acagatcaaa ccaatcagca aaatcaattt gttcacatga tattttttcc agatgcgctt
    25141 tcgccatcga tggaattgtc cgagatatgg tctgatccaa cgccaaaaac tgatcaaagc
    25201 agcttttatc ttttctaaaa tgctttgaaa atccatttgc ttcttctcct gtctatttga
    25261 ttcaatcgtc ttattttacc atagtgaaag agtgttttct tcctgcttaa gttgtagttc
    25321 aatatgtttt tttacgttag tgcaagtaga gtaacataag aatgaacaga aaaatgtaag
    25381 ttttttctta aatatgtttt aaaaatggta caattagtct gcgattgcac tgaaaggagt
    25441 acacttgtga aaatcacgat cactttacct atgaaccatc ctgaaacaac aattcgcgac
    25501 ttgcttgaaa aggaatggtt agttcctaga aaggttcgac actttttgcg tatacgtaaa
    25561 aacgtttgga tcaatcaaca acctgctttg ttccatcatg aagtaaaggg caatgaccag
    25621 atcacattgt tattcgaaga aacagattat aattatcaag aagttcaatt aggagatgcg
    25681 aagcacatcc gtgttttata tgaagatgag catttgatta tagtggataa gcctgcagga
    25741 atgaagactc atccaaatga accgacagaa aacagtacct tactcaatca tttagctgct
    25801 tatctcgaca ctaaacagca acgaccttac gttgtccacc gcttggacaa agaaacaagc
    25861 ggtgccgttt tatttgcaaa aaatccattt gtcttgccga tacttggaag aatgttggaa
    25921 caaaaaatga tctatcggag atatcaagcc atcgtatggg gagaaattgc caaagatatg
    25981 accatcacca ataaaattgg acgagatcgc catgatcgga gaaaaagggt cgtagatgat
    26041 aaaaatggtc agagcgcctt gacacatgtc actgtcaaca aagtactttc tgaacaaaca
    26101 caagtctatt gtgttttgga aaccggacgt acacaccaga tcagagttca tttagctcat
    26161 aagggacatc cgatcgttgg agaccctctc taccaaaata gaacagctga acgactgatg
    26221 ctccatgcat tagaactgca catgatccat ccatttactc aagaaaaaat agtcggtaaa
    26281 gctttacctg gattatggga atagaaataa aaaagaacag agtcttatca gcagataatg
    26341 ataagactct gttctttttg tgtaatttgt ctgcttactt ttgatgttcg tgttctgcta
    26401 acagcaaact accgataatc ccactttcat tcggcagtcc ccaacgaacg atatattcac
    26461 ttacttgagg tgtttccata taaccaccca tcaattcaac aaattgctga cggatttttt
    26521 gaaggagatg atcctgattc attaccccac cgcctaaaat aatcttttca ggtgctaacg
    26581 tcaacgtata gttgatcagt gcttgagcaa tataataagc ctgaatagcc caaacaggat
    26641 gatcttgcgg cagatcttgc cctttgatcc ctgtgcgtgc ctcaatagac ggtcctgctg
    26701 ctaatccttc taaacaatct ttatgataag gacaagtacc ttcataatgg tcatctggat
    26761 ggcgtttcac ccaaatatgt cccatttcag gatgtgcgat ccctgaaaaa atttcttcat
    26821 tgatcacgac acctgcaccg attcctgttc ccacggtaag gtaaatacaa ctattttttc
    26881 cttgtgctgc tccttgcttc aattccccat atgcagcagc attgacatcc gttgtccaag
    26941 cagcaggaat attgtagcgt tccttgattg tgccaagaaa atcgaattgt gcccaccctt
    27001 ttttgggagt tgccaaaacg tatccgtaat tatcttttgt tttgtcgata ccaatcgggc
    27061 cgaatgagcc aatcccgatt cccttcaatt cataacgatc aaaaaattca aatacctgat
    27121 ccaatgtttc ttctggtgtt gtggtaggga tacttaattt ttctaaaatt tctccaccat
    27181 ctgaaaccgc acacacaaat tttgttccgc ctgcttctat tccgccatac attcgttttc
    27241 ctcctcgaat ccgattgtcc gaatctcata aggttggatt attgggacaa ccgcttgctt
    27301 cgttggttct tcaagcagat tcaatatgac agtttttcca ttgtctttag ttatttccaa
    27361 tttttctaaa tgactagaca tattgaaacc tcttactact actttatcac taaacttgct
    27421 gcgtttcaaa gctgtaattg caaacgtttc actttttatc gttaaatatt gttgttttga
    27481 aatcaatgtt ccttcatgat gtttgatttg ttgagtggaa aaaggaatct gtgctgcgta
    27541 tgcatgcaaa taagtagaga atttttcttc tggtccatgt agttcaatgc tgtaattgaa
    27601 ccgatgctca cctagacact gtgcttctgg tgttggaaaa tatccccaat cgcctagttc
    27661 tcccacactg cgaagtagcg tcactgcgat ttgtccgtct tctgttactt catattcatt
    27721 caaaccaaag tttccgactg ttacaccgta ctcttcatca tgaatattga caaatgcttg
    27781 ctgatgttgc ggattcgtgg ggttttccca acttttagac acatggtttg ggcgcttcac
    27841 cacttcaaaa atactatctg cttcgtgatg ttccacatgt aactttgtag gaaacaagac
    27901 acgtaagcga tgatctttca tctgattgtc gagagtcgtt tcaaagtcga tttttttact
    27961 atcttttctg attgtcatga ttgtttttat ttgaaggatt cgtttttctt tggatcgttc
    28021 tgcttttcga taacgaaatt ccacaaccat ctgctgttct ttttctaaac gctcgtctgc
    28081 agaaacaggg atttccattt cctgtagaat acttattttt gctatttccg gtgtatctac
    28141 taatactttt acttcagagt tttctttgtt actggatagg attggctgat cacagaaagg
    28201 ttgtttaaag atatactcat ttcctatatc gccgatatct tcaaaaatat gcagattatt
    28261 caactttcgt ttgtttttgc gatcttccat tgtaatggta ccattttttt caatcgtcaa
    28321 atgtagaggg ccgttttcga tgattcgtcc actctgatga atcattgttt ccttttcttc
    28381 tgctttcgct tccccttcta caagcgcgaa tgtttcccaa gaaaatgctg gcatgttttc
    28441 taatggtaaa gtcactttga tcattcttgc catatatggc acacggaaac ggtctttcgg
    28501 caaatcataa ccaaattgga cagtttcttc agataaaata gctggtatag tggccccact
    28561 ttttgtttct acatgatact tacgtttagg caaattttct aactcttgat atagttgttc
    28621 aggtattcct tcagcgaatc tttttctttc taattcgatg gttatttctg cttcaccagt
    28681 tttatccatt cctgcagtat ttacaatgac aaatggaaag ctgtcttttg ggaatccggt
    28741 cgtatcgatt gctgcggtca gctgttctaa ggaatcttgt gcaagatact ttccaacttc
    28801 atctgctttt tcaaaacgag gtatcatttc ccgatggaca gaatcgactg aacaaccgca
    28861 aatactgtcg tgaggatgat tttgcattaa tgtcttccaa gcataatcta attgatcatg
    28921 gggatagttt ccactaactt cataagccat cgtcgcaagt ggttctgtaa tattttctag
    28981 ctgtcgctgg accttggtat tccattgttt taagtaaacc cgagcagatg ctgtattggc
    29041 taaagtatac cagccatccg tttcttgact tgtcaattct ccttctactg agcctaagtc
    29101 ttctggcaca tctttttgaa ctgcttccag atagtcagta aaattagaat gaataaactc
    29161 gtaatctgga aacagttcat ttgcgagatg gattgctttt gatatatctt tttgtaccgg
    29221 ttgatgatca acaccattca tcatcaataa gtggttcgta gaggcatatt gttctgcatc
    29281 cgctaatttt tgcttccaaa aggcaagagc tgcttctttt tctactggaa tctcattccc
    29341 gttgctatac caattggcaa atagcagacc aaagattgct gtttgatcag gtcctttcca
    29401 ccacatctct gagtattggg aagaatagtt ttctgcttcc aaaacttgat tatcaaagcc
    29461 aattggtttt acaccacggc caaacgctgc tgctgaaata ccagcttgtt tcattaattg
    29521 aggcgtctgt cccatatttc caaatgtatc gggaaaatag cctaacatga caggggttcc
    29581 ccatttacgg ctttcttcca tgccaatcag catatttctc acgttcgatt ccgagctgat
    29641 aagaaaatca tcttgcaaga tataaaatgg tccaattctc aattttcctt cattgattgc
    29701 ttgttggatt gcctgtcttt tttctggcct tacttgaaga tagtcatcta aaataatcgt
    29761 ttgcccatcc aaatgaaaac tgttgaagga cggatcaact tcaaatagct ctagtagatc
    29821 gtctatcaat tcaatcagtc gcatgtgatg ctgttcgtaa gccatgtacc attcacgatc
    29881 ccagtggcta tgagaaatga tatatacttt ctttttcatt tacgatacct cctacttttc
    29941 tactctaata tcaaagtaat ccatgactaa ttcgcaaaac atcatatttg cccaagaaaa
    30001 ccattctctc gtataatttt caggattgtt cacatcgaat ccttcgtgca taaggtgtgt
    30061 tcctgcgtca gttgctacaa gtaaattcaa tatccgctct ttttcagatt tgtctgacgt
    30121 tgtcattcct tccatagcta atgcaatcgg ccaaacgtat ttttctggcg tatgggagga
    30181 tccaattcct tttgcgtact ttccttcata gaaatatgga ttttctttac tcaacaatgt
    30241 tcttcttgtt tgcagataat cttcctcttg ttcagaacaa tatcccaagt aaggtgcggc
    30301 gatcagattg ggcacattac tgtcatccat gactgtggcg tttcccattc catctacttc
    30361 atatgcataa atcgtttgcc ctttttgatt aaccgttcgt ccgtgattct gaatcccctc
    30421 ttggatctct ttcttcaatt tctgtgtttg ctgtacaatt tctttatcat cgagtatcgt
    30481 ttgaaaaatt tcttccacat aacctaacac gaccaccgca aacatattgg atggtaccag
    30541 atatccataa cggcaagtat catcgctagg acggaaacca gaccatgtca ttcctgtaaa
    30601 agctgttttt gcacctcgcc cgtcattgat cagcgtatct tcttttcttg tcgtgtctcg
    30661 tacaaatgta taaggagagt gttcatgatc ttgctctgtt ttgaacacgt ccaagatttt
    30721 tttgatacct tcaataaaag attgattgaa ttgatcagtt tttcctgtat ttttgtaaag
    30781 aagataggcc aactgtattg ggtagcataa agaatcaatt tcatattttc tttcccagat
    30841 ccaatcgttc atttttgtat gatctgtttg gtggccagca ccgttagctt cttcgttgaa
    30901 ggcattcgca tatggatcga tattgatata aaaaaattgc tttttgacta acccgctgat
    30961 catgtcagct aattcctcat cttcttttgc aatcactaaa taaggacgaa cttgtgcagt
    31021 agagtctctt aaccacatcg ctggaatgtc tcccgtcaat aaaaaggtgg ttccgtcatt
    31081 ttgctttttg actgttgtca acagagtatt ggcaaatgct gctgtaaaat tctttgccca
    31141 gtccgcatgg atatccccac atttttgtgt gatatcttcc ataaatttct gcaccgattt
    31201 tggtatatct ttataagcca ctaataaaac cttctttcta tttg
//
LOCUS       DAZKNO010000009        30062 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000009 DAZKNO010000000
VERSION     DAZKNO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.009"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1208)
                     /locus_tag="ACG8MY_000297"
     CDS             complement(159..1208)
                     /locus_tag="ACG8MY_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0429530.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFTRGAS"
     gene            complement(1290..1979)
                     /locus_tag="ACG8MY_000298"
     CDS             complement(1290..1979)
                     /locus_tag="ACG8MY_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0429531.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(2085..2453)
                     /gene="rplL"
                     /locus_tag="ACG8MY_000299"
     CDS             complement(2085..2453)
                     /gene="rplL"
                     /locus_tag="ACG8MY_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0429532.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2506..3009)
                     /gene="rplJ"
                     /locus_tag="ACG8MY_000300"
     CDS             complement(2506..3009)
                     /gene="rplJ"
                     /locus_tag="ACG8MY_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0429533.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3213..3340)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3419..4108)
                     /gene="rplA"
                     /locus_tag="ACG8MY_000301"
     CDS             complement(3419..4108)
                     /gene="rplA"
                     /locus_tag="ACG8MY_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0429534.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4212..4634)
                     /gene="rplK"
                     /locus_tag="ACG8MY_000302"
     CDS             complement(4212..4634)
                     /gene="rplK"
                     /locus_tag="ACG8MY_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0429535.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4977..5645
                     /gene="sdaAB"
                     /locus_tag="ACG8MY_000303"
     CDS             4977..5645
                     /gene="sdaAB"
                     /locus_tag="ACG8MY_000303"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0429536.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5658..6530
                     /gene="sdaAA"
                     /locus_tag="ACG8MY_000304"
     CDS             5658..6530
                     /gene="sdaAA"
                     /locus_tag="ACG8MY_000304"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0429537.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6621..7721)
                     /locus_tag="ACG8MY_000305"
                     /pseudo
     CDS             complement(6621..7721)
                     /locus_tag="ACG8MY_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7813..8700)
                     /locus_tag="ACG8MY_000306"
     CDS             complement(7813..8700)
                     /locus_tag="ACG8MY_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0429538.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8777..9322)
                     /gene="nusG"
                     /locus_tag="ACG8MY_000307"
     CDS             complement(8777..9322)
                     /gene="nusG"
                     /locus_tag="ACG8MY_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0429539.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9423..9593)
                     /gene="secE"
                     /locus_tag="ACG8MY_000308"
     CDS             complement(9423..9593)
                     /gene="secE"
                     /locus_tag="ACG8MY_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0429540.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9617..9769)
                     /gene="rpmG"
                     /locus_tag="ACG8MY_000309"
     CDS             complement(9617..9769)
                     /gene="rpmG"
                     /locus_tag="ACG8MY_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0429541.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9931..10575
                     /gene="cbpA"
                     /locus_tag="ACG8MY_000310"
     CDS             9931..10575
                     /gene="cbpA"
                     /locus_tag="ACG8MY_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0429542.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10631..11134)
                     /locus_tag="ACG8MY_000311"
     CDS             complement(10631..11134)
                     /locus_tag="ACG8MY_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0429543.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11147..11193)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11731..12696
                     /locus_tag="ACG8MY_000312"
     CDS             11731..12696
                     /locus_tag="ACG8MY_000312"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0429544.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12795..13967)
                     /locus_tag="ACG8MY_000313"
     CDS             complement(12795..13967)
                     /locus_tag="ACG8MY_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429545.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13954..15099)
                     /locus_tag="ACG8MY_000314"
     CDS             complement(13954..15099)
                     /locus_tag="ACG8MY_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429546.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(15113..16045)
                     /locus_tag="ACG8MY_000315"
     CDS             complement(15113..16045)
                     /locus_tag="ACG8MY_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429547.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            16162..17241
                     /locus_tag="ACG8MY_000316"
     CDS             16162..17241
                     /locus_tag="ACG8MY_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0429548.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17238..17867
                     /locus_tag="ACG8MY_000317"
     CDS             17238..17867
                     /locus_tag="ACG8MY_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0429549.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17977..18204)
                     /locus_tag="ACG8MY_000318"
     CDS             complement(17977..18204)
                     /locus_tag="ACG8MY_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0429550.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18307..20895)
                     /locus_tag="ACG8MY_000319"
     CDS             complement(18307..20895)
                     /locus_tag="ACG8MY_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0429551.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20915..21574)
                     /locus_tag="ACG8MY_000320"
     CDS             complement(20915..21574)
                     /locus_tag="ACG8MY_000320"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0429552.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21623..22126)
                     /gene="trmL"
                     /locus_tag="ACG8MY_000321"
     CDS             complement(21623..22126)
                     /gene="trmL"
                     /locus_tag="ACG8MY_000321"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0429553.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22347..23213)
                     /locus_tag="ACG8MY_000322"
     CDS             complement(22347..23213)
                     /locus_tag="ACG8MY_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0429554.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23322..23963
                     /locus_tag="ACG8MY_000323"
     CDS             23322..23963
                     /locus_tag="ACG8MY_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0429555.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(24025..25386)
                     /gene="mgtE"
                     /locus_tag="ACG8MY_000324"
     CDS             complement(24025..25386)
                     /gene="mgtE"
                     /locus_tag="ACG8MY_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0429556.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25404..26303)
                     /locus_tag="ACG8MY_000325"
     CDS             complement(25404..26303)
                     /locus_tag="ACG8MY_000325"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0429557.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26305..27102)
                     /locus_tag="ACG8MY_000326"
     CDS             complement(26305..27102)
                     /locus_tag="ACG8MY_000326"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0429558.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(27080..27784)
                     /locus_tag="ACG8MY_000327"
     CDS             complement(27080..27784)
                     /locus_tag="ACG8MY_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0429559.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27915..28490
                     /locus_tag="ACG8MY_000328"
     CDS             27915..28490
                     /locus_tag="ACG8MY_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFQ0429560.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28608..29285
                     /locus_tag="ACG8MY_000329"
     CDS             28608..29285
                     /locus_tag="ACG8MY_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0429561.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(29375..>30062)
                     /locus_tag="ACG8MY_000330"
     CDS             complement(29375..>30062)
                     /locus_tag="ACG8MY_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0429562.1"
                     /translation="LERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDR
                     FHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVW
                     RASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLP
                     ETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIM
                     IRHGKTFLTK"
BASE COUNT         8847 a         6335 c         5417 g         9463 t
ORIGIN      
        1 ctcataattt ccttgaatgt tataaaagta tctgtataat gttcagtata ttttgatgaa
       61 cgcatatgta gtcctcattt cttgggagtt ttgagactac tatatgcgtt tttttgttga
      121 tttaccaaca tgtttagaca ttttcctcta ggaaaaaatt aactagcacc acgggtaaat
      181 acagtattat ttcggttgct tgctgcttca cctagccctt gtactcgtgt ttgattggcg
      241 acttgaccaa attgattccc gacgtctaat aaaataatag ctaaaagcaa aatggccaaa
      301 tggcttccgc caaccaataa agcaataaag cttattgttt gcataacaag tcccatatac
      361 acgatttttc tttcagaata agaatctgcc agtcgtccca cgataggtgc agcaactgct
      421 ccagacaaac caaaaatcgc taatatccct gcttcgaatg ttccccaatt gtaggcggga
      481 ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa atgttccaaa catgaaaaat
      541 ccactgactg ccgcttctct taatagacgt tgagaagcaa ctaaatgcgg caaactttta
      601 agtgaatcaa ggtatgaaac agctgtacta ctttgctgga cagaacgcgg cattttccag
      661 taaagcagaa gtgtaagaat tccgactgca cataaagcaa ataaatagat catccgccaa
      721 gaaaaaagac tggcaacaat accagatacc gtccgagaaa gcaaaatccc cacgagtaaa
      781 ccgctaagca atgtacccat gaccttccct cttgcttttg gtcccgctag taccgcaccg
      841 taaggaatga tgatttgcgg cacgatcgaa agtaatccta ttaaaaaaga agcgcatgca
      901 aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag agacggcaga taacgctacc
      961 attcgaatga tcaattttgg acgatccacc acgtccccta aaggaactaa aaacagtaac
     1021 cctaacgcat agcctaattg ggtcaacatc gtcaatatcc cgactgcact tgtgctaaca
     1081 gataaactag ctgctacttt tgttccaatc ggctggatgt aatacatatt ggcaacaaca
     1141 acgccacaag tgattgataa tagtaaaata agcgaagttg ttaaaggtgt tttttcatta
     1201 ctcttcattg atttccccca tttcctatct tagtttacaa cttatggaaa agattggaaa
     1261 tagaaaaata caactattcg ccacacttat tatttgtgct tttgttttcc ttgttttttc
     1321 ttgcgatctt tataaatgaa gacaactaac cctactagca cagtaacact tgcaaccaac
     1381 gtgactgcag ttttgttata tccgtgaaac agagcttctc caccacacgc atacaacaca
     1441 gacgaaggga tgactccaat cacgatcgac aatatgattt gctgtagagg caatttcatc
     1501 gcatctgcag caaaattgac catagaagat ggaataaccg gtatcatata gcctaacatt
     1561 acacctattt ttggatgttt catttgtcgg attgcttgca cccaatgatt cgtttcggtt
     1621 ttcttatcta aaaattttaa gcgatgcata aggaaaattg cagtgatgtt accaagagca
     1681 ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa agctcaaacc agcaatgact
     1741 ccaactactg aagtaggaat acctggaaca gcacacatca ccgctgtcaa tacgatcaag
     1801 agtatcgatg caaaagtccc atgcgaacga atatgtgcga gtagctctgc cttactcgct
     1861 ctcggattta aaaataattg gatgtctgtc tgatattcta aatacaaccg atagcccact
     1921 accagtatca gaatgattcc caacgagatc aacatcaatc ggattatttt ctttttcata
     1981 tttcctcctg aaaaaaatct atattcatta aataatagtt taacataaaa acaaaaaagc
     2041 accgaaatcc aacataggat ctcagtgctt ttaaatagta aaaattattt aacagttaca
     2101 gatgcgccaa cttcttcaag tttagctttc aattcttcag cttcttcttt agatacgcct
     2161 tctttaactg gtgcaggagc gccatcaact acagctttag cttctttcaa gcctaaacca
     2221 gttgcttcac gaactgcttt gataacttta actttttggt ctccagctgc agttaattct
     2281 acagtgaatt ctgtttgttc ttcagcagca gcagcgccac cagcagcagc tacagctaca
     2341 ggagcagcag cagatacgcc aaattcttct tcaatagctt taactaggtc gttaagttct
     2401 aggatagttg cttcttttaa ctcagcaacg atgttttcaa tgttcaatgc cattataatt
     2461 tcctccgttt aattgttctt ttttatttaa tgctttatgc ggcaattaag ctgcatctcc
     2521 atcttctttg tctgccactg ctttgacagc gtaagccacg ttgcggactg gcgcttgtag
     2581 tacagatagc aacatagata gaagtccttc gcgatttggc aattttgcca atgctgtgat
     2641 ttcttccaat gaagagactt taccttcgat gataccgcct ttgatttcta atgcttttgc
     2701 ttctttagca aattcgtcca tgattttcgc tggtgcgact acatcttcgt tactgaatgc
     2761 aacagctgta ggtccagtga atacttcgtc catgccttca aggccagctt ttttagctgc
     2821 acgtgaaaga atagaatttt tgataacttt catttctacg ttagcatcac gaagttgttt
     2881 acgtaaacgt gttacttcat caactgttaa tccgcggtag tcaacgacta ctactgatgc
     2941 agctgctgta aatttttcgc ttacttcatc aacgattgct gctttttttg cgataattgc
     3001 ttcactcatt tttgagtttc acctcctgaa ttttgatgga agagtttttt gcactgaaaa
     3061 ggcaagtcac ttttatagtt actttgctca accagtatta ttcatcacct attcacttcg
     3121 ttcatagtga ttctactcgc tttgctcgaa aggaaactcc tttcgctttg ctcaaccagt
     3181 attgttcatc atctcttcac ttcgttcata gtgattcaca ataaaaaact ctatgccacc
     3241 gcagacatag agggactatt gaatatattc aataaacgtc ctcggtagga aattaaggct
     3301 tgatgccacc tactgtcttc ggtacaagtt aattattaac ctcaactagc ttagcatgct
     3361 aaactagttg agtcaagaat tatttttcag ttggatcaat caacattcta atatttaatt
     3421 agaaagaagc ttggtcaaca tggataccag gtccaaatgt tgttgtgatc gttacatttt
     3481 tcatgtattg acctttagct gctgatggtt tagcttttag caatacatca tggattgctg
     3541 caaagttttc aactaatttt tcatcgtcaa atgaaacttt tccgattggt acatggatat
     3601 ttccagcttt gtccacacgg taagttactt taccagcttt cacttcgttt actgcttttg
     3661 ttacgtccat tgtaactgta ccagttttag ggtttggcat taagcctttt ggtcctaata
     3721 ctcgtcctag acgacctaca gtagccatca tatcaggtgt tgcaacaact acatcaaagt
     3781 caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag
     3841 ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac
     3901 cagttccgtt tggtaataca acagcaccac ggatttgttg gtctgctttt ttagggtcta
     3961 cgttcaaacg gtatgcaact tcaactgttg catcaaattt tgcaatgttt gtttcttttg
     4021 ctaaagctac tgcttcggca acaggataaa ctttacttga atcaactttt tttaatgcat
     4081 cttgcatttt tttgctcttt ttagccattt tggttcctcc ttgattgtgg ttataacggt
     4141 tgtacctccc acgtgttcta tatctttcaa tatagagcca actgagaagc tgctgtctta
     4201 gggtgtatag attattctac agtgatcccc atgcttcgtg cagttccttc aaccatgcgc
     4261 attgctgctt caacgttagc tgcgtttagg tcttccattt ttaattcagc gatttctctt
     4321 acttgatcgc tagaaacttt cgcaacttta tttttgtttg gttcgcctga acctttttcg
     4381 attttagctg cttttttcaa tagaacagca gctggcggtg tttttgtaac gaatgtgaat
     4441 gaacggtctt cgtatacaga aataacaacc ggaatgatca aacctgcttg atcagctgta
     4501 cgagcgttga attcttttgt gaatcccatg atattgatac ccgcttgacc tagtgcagga
     4561 cctactggtg gagctggagt tgctttacct gcaggaattt gcaatttaac gattttttct
     4621 actttctttg ccacgagaca tacctcctta agtccgtgat gtggttaatt ggagatgcat
     4681 atttctcctc ccacgtttta tctgatctga aaaaatccag acaaataatg tgcaaaacaa
     4741 tgcacactgg tacagtatag caaatttatt tgttttttca agattttttt ctgctatttt
     4801 tacattttgc ttgaataaac gctaaattta ttctaaaaaa acaacaattt ttcgttctca
     4861 tttcttcttt tatgttacac ttgcgctagc atggttcaac agatgaaagc aaacaataac
     4921 acggtttttt agacccttgt acaaagattc ctacataaca aaatgaggtg gagaaaatga
     4981 aagatttaag gtatagaagt gtctttgata ttattggacc tgtgatgatt ggtccaagta
     5041 gctctcacac tgccggagct gctagaatcg ggaaaatcat acgtagtata tttggcgaac
     5101 agcctgattc tgtagatatt tatctatatg aatcatttgc aaaaacctat cgggggcacg
     5161 gaacagatat tgcattagtt ggcggcctgt tagggatgga acccgatgat gaacgtttag
     5221 ctgattcttt gaaattggca tatgaagccg gtatggaagt cttgtttgtt cctaagagtg
     5281 aaaaagccga acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt
     5341 cagtcactgg catttcgatc ggcggtggca atatccagat ttctgaattg aatggattca
     5401 aaatttcttt aagcatgggt actcctacct tgatcgttgt ccaccaagat gttccaggta
     5461 tgattgctaa agtgactagt atactttctg agaccaatac gaatatcggc actatgacag
     5521 tgacacggga atcaaaaggt gaaaatgcca ttatgataat tgaagtagat gatcctcaag
     5581 tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta aattatttcg
     5641 attaaggagc taaaaaaatg tttttatcga ttgaagaatt agtagatcaa gcaaaagctt
     5701 atccaagtgt agcagaattg atgattgcag ttgaaatgga aatgagtggc cgttcccgta
     5761 gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg
     5821 gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg
     5881 attatttaaa tgaggcagac tttttaagtg gagaaacaat cttaaaagct gtacgcaatg
     5941 ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg
     6001 gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg agatcggctt gatttgacac
     6061 atgaacagca attggaattc ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca
     6121 acgcttctat cagtggtgca gaagggggat gtcaagcaga agttggttct gcaagtgcaa
     6181 tggcagcagc agctttggtt tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag
     6241 tagccattac attgaaaaat atgatgggac tgatttgcga tcccgttgca ggcttagttg
     6301 aagtcccgtg tgtcaaacgt aatgctttag gctcttctca agcttttatc tctgctgata
     6361 tggcattagc gggtatcgaa agtgtcattc cgccggatga agtcgttgct gcaatgtacc
     6421 aagtcggacg tcaaatgccg tcgattttca aagaaacagc agaaggtggc ttagctgtta
     6481 cgccaactgc taaacgactt gcggaagaga ttttaaataa gagggcgtga taaagctcct
     6541 taatttcgag tattaagaga ttagttatct tcataacaaa aaacgaagag aggactaatt
     6601 gcccttctct tcgtttttta ttaatccatc aatttcgtat tttttacttc atcgatcgtt
     6661 tttgttcctg caagttgcat cgtgatcgac aattctttgt tcaggtgttc aaaaacagat
     6721 gttactcctt ctgccccacc tagattcaac ccataaatca ctggtcggcc aatcgcaact
     6781 aagtcagcac cacttgccag agctttgaag acatgctctc ctcgacggac accagagtcg
     6841 aaaatcactg gtacacgttt gttgactact tcggcaattt ttggcaatac ttcaaaggac
     6901 gcaggacctc catctaattg acgtccacca tgattggata cccagatacc atctgcccct
     6961 gcagagatag cgattactgc atcttctggc gattgtattc cttttacgat aactggaaga
     7021 tgggtgattt ctttgattgt tttgatgtca tctggtgtca agccttgttt tgcagctgca
     7081 taaatttctg cgattccttt tccttcgcca ttaccacttg cactttgttc gctatacgca
     7141 gctaagttag gcattggtaa tggaaattgg aattgattga tgacgtcttc ttcacggtaa
     7201 ccaccgagcg ttgaatctgc cgttaaaatg atagctttag cccctgcttc tactgctttg
     7261 ttcaaaatga actcattgaa tctgtcgtct ttactcatat ataattggaa aaactgcggt
     7321 gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt gctgatggaa
     7381 aagattgatc cagcagcagc tactccttta gctgtatcag cttccccttt ttcatgtgcc
     7441 aatccttggg ccgctgatgg cgcttggata atcggtgtat ctaattcaat tccgaaaaca
     7501 gatgtatgca aatcggctga gtcgattccg cgtaatatcc gtggcatgat tttctttgta
     7561 ttaaaagaag tcgtattttc tttcatcgtc cactcatctt cagaaccacc gcgaatatag
     7621 ccaaaagctc ctgcttccat gcgctctttt acacgacctt ctagttaggc aatgtttaca
     7681 atatcgattg gatgttcttc attacttgta tgataagtca tagtagatcc ttctttcatc
     7741 ttcttgatta catataaatc atactaaact tacaaaaagt aagcaaacat ttactctcag
     7801 atacttttat cgctattctt tccaaataaa tcgacttaca agctgatcta cttcttggtt
     7861 ttcatgaagc atactatgag aggcattttc acctgtcact actttttcct ctatttcatt
     7921 tccgcgttgc ttcaacaacg catagaccgc taatgcactg tttaacggta ctgttccatc
     7981 actcaagtct gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg ttataggaat
     8041 attaccgatc atttgctggt aatcttgata gcggttagaa acgacagcag gcccctttgc
     8101 taactcatct gtcagagact gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat
     8161 tgctgttaat gtctgaactt gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg
     8221 tagtgcacta actcctccca tggaatgccc cactatgttg actttatcta tgccgtagtt
     8281 ttctttcaga tacaaaagac aagttttgat ccattctgct tgattccact cgttattttt
     8341 attatcttca aacaatactt ggaccatcgg attgtctgct tttccagaca aatctcctgt
     8401 tgcttgtatt tgtccatctg ttcctactgt taaaaccaat tcttttactg cttgctgatt
     8461 tttttccaaa cgccgcaaca tccctttaaa ggaattttct gtcccgctgt agccgtggaa
     8521 aaaaagtgtt ggaatagtgg ttttttctat cactttactg gattctgccg ttttttcttt
     8581 atcagaaact tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg caaacaaact
     8641 gataaggact aaagctgctg cagttgcacg tttgcataaa tttttgattg gttctgtcat
     8701 acacctcttc ctttccatat agaaaaaaga aagacttgaa aggccaatca tatgatcgac
     8761 atttcaagtc tctttattaa tcgattttat ctacttgatc gaaatcaagt tctgtactgg
     8821 tttcacgtcc aaacatatcg atgttgactt tcaatttttc tttttctccg tcaacttctg
     8881 ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat tttcacgact tcgccaactt
     8941 ctacttctag atcattttgt cgcaggctca taccgataga acgcaagata tgattgactt
     9001 cttctggaag taatggtgct ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca
     9061 caccaggtgt gttgcggaca acataccatg aagcgtctgt catgatcatt tcaacaagta
     9121 cgtatcctgg gaatgtttta tgaacgattt ctttttcttt cccatttttg acttcagttt
     9181 ctttttcttc aggaacaacg acacggaaaa taaagtcggc cattttcata ctttgcgcac
     9241 gagattcgat atttgctttt actttgtttt catatcctga atatgtatgc aagacatacc
     9301 agtttttttc aaaagattcc attgaaggct cctcatttca ttgtaaaata aaaaaacctc
     9361 agtacacccg aagtttttct ttcgcttcat tatagcacta aaagtacgtt gtggctagcg
     9421 agttatttta aaatccagcc aaataagctt tggatggctg tatccattac gtaaaacatc
     9481 aatccgaaaa taatagaagt ttcgatgact actaacgtat ctttacggag ttgttttctg
     9541 cttggccaag ttacttgttt gatttcatct tttacgcttt ttagaaattt catgagacaa
     9601 cctcccttgc tactgtttat ttcgtttctt tatgtaatgt atattgatta caatatttac
     9661 aaaacttatt gatttccaaa cgttcgccgc tttttccttc actgactgat tttgtgtagt
     9721 tgcgagaccc acaaacggaa caagcgagtg ctgctttttt ctttgccatt tacttctgcc
     9781 cccttgctga gaatcagact tatacttcat aacagtatca cgtaactttt tacttgtcaa
     9841 tttgtgaaat aaactatcta ttgttttgga taaaattatg atatgattaa tttgaaatta
     9901 agctaaaatg agacaattgg aggtatagcg atgctattaa aaacacttgt atataagaaa
     9961 aaagatctga caaccgttac agagactagt acattagaag aagcattaaa aattttagaa
    10021 acttccggat accgttgtgt gccgatctta gattcttcag gtaatatttt ccgtggaaac
    10081 atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc
    10141 actcatcttt tgaaaaacgc tacaaagttc atttacctgg atacttcttt cttcaaagta
    10201 ttctttacga tcaaagagct tccttatatt gctgtccttg acagtgaaaa tcatttttat
    10261 ggtattttga cacatagtac gttgcttaat atgctctctc aatcttggag cgtagaacaa
    10321 ggaagctatg tactgacaat tgcttccaca ggaaagcaag gagatttggc tgctattgca
    10381 aaaatcattt caaaacacag cagcattgcc agctgtatta ctcttgatat cgaaaaagac
    10441 gaatacattc gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa
    10501 acgatcgtca accacttaga gaaaaagaat tataaagtgg tagaaattga aaatttgcaa
    10561 aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc taatctgctt tcttttttcg
    10621 ctttttataa ttagacacgc ttttcaaaat caagcgcgcg atctaaataa tacatcaatg
    10681 gtgctgaaac agccatgacg ataaactcac ttaatgcaag cgtgccatag gttgtccaaa
    10741 atgcttggcc acctgttgga actaacatat aagcaatcag tcccatgctg atggtaaaga
    10801 agatcgtgtt caatactaaa cgcatcttta catttttcac tttgttttgc aataaagctg
    10861 tcaggcctaa agaaagcaat gtttggccgc caccaaaaac cacatccatg ataccaaaac
    10921 caaagaaggc attatagacg atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa
    10981 agacaactaa atgattcaaa ctttctgaaa tacgaaactg gatcggccct gaggatacag
    11041 gcgcaactaa aatagttaac gctagataaa gagccatgat gatgccattt acaacgatta
    11101 cggataaccg ttgtctgctt ttcgtttttt gcattttctc tcctcttttc tcctagtttt
    11161 tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac aaacaataat tataacagac
    11221 ttttcattta tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta
    11281 aacgtttgct aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc ataagaattc
    11341 ccagtaaagc agacgaagta ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat
    11401 aaaaaaggat gtgggaattt gagcaacctc ttactagaga acggtctagc aagcagctga
    11461 agatctaagt ggacatgccg aaccttggaa cttctcctta cgtaagtaat ggatcatgcg
    11521 cttgctaggc ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg
    11581 ctataataac caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat
    11641 tcaaaaaata gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata
    11701 tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat
    11761 ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa
    11821 aataagcata ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt
    11881 gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc
    11941 gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat
    12001 actttctatc agatggaagt agaattccca gttgcttata ttccagcagg ttctggaaat
    12061 gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta
    12121 gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa
    12181 aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca
    12241 aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta
    12301 ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga
    12361 ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt
    12421 ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc
    12481 gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa
    12541 aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca
    12601 acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc
    12661 acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta
    12721 catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt
    12781 tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta
    12841 cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg
    12901 aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta
    12961 ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag
    13021 tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg
    13081 atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg
    13141 atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag
    13201 gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa
    13261 ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg
    13321 tttcgtttac tgatctcttt gcgattaaac gctagattga tgacgctgaa accgctaaaa
    13381 atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc
    13441 atccgctgac tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt
    13501 gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc
    13561 aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt
    13621 atcttgatcg tcgccatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt
    13681 aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca
    13741 tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc
    13801 gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca
    13861 atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc
    13921 gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct
    13981 ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt
    14041 ggtataaaaa ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa
    14101 gttgacgaga tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg
    14161 ttccgacccc atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct
    14221 atccttttgc accagattca tttttggtat caaattacca accaatgtac ctaacaaaat
    14281 cgcacacaac gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc
    14341 tacttgatag ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt
    14401 aaagcttgct gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa
    14461 gcgaatacca tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat
    14521 cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat
    14581 attacgacct gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg
    14641 cgcgagatca acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa
    14701 aaattgatcc atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag
    14761 cgaaacagat gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc
    14821 attcgcttct acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac
    14881 ttgttctaag actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat
    14941 tccagtatta atcgtttcaa gattgccacc ttgatcaatc tcgccaaagg ccgccataaa
    15001 catcaatcct aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag
    15061 cagtattttt gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc
    15121 tcaaatcctt gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta
    15181 tttttccgta actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat
    15241 ttagttcagt agttgttact tggagctgac cttttttata agcgattcca tcagaagcta
    15301 ttgttcgctg aatcgctgac aacacatttt ctgggagtaa tgggatgtcg atcgtcaaac
    15361 gatccgccgt ccgtaccata tcttttagag attctttggt tcctttagca atcacctgtc
    15421 cttgatccat gatcacgatc tggtcgcaaa gaatttctac ttcttccatg taatgtgttg
    15481 tatagacaat ggttgctcct tgtcgattca gctccttgat actttctaaa attttattcc
    15541 gactttgggg gtctacagct actgtcggct catctaaaaa aattaaccgc ggcttgtggg
    15601 caatcccaca tgcgatgttc aaccgacgtt tcaagccacc actaagttct cctggacgaa
    15661 acgaaacata tttttctaat cccactaatt gaatcacctc ttcaatcaag gctttccttt
    15721 tctctttttc ggaaacatat aaaccgcaaa aataatcgat attttcttgc acacttagtt
    15781 cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat tgccttgaca gcataatttt
    15841 caggagtcat tgttgtcccg aacaatttaa tggttccttt gtcataattt aacaaagaaa
    15901 gcatacagtt gatggcagtc gatttcccac tgccgtttgg acctaacaga cctaagactt
    15961 ctccttcttg tacttgcaga tcaaaatgat tcaatgctac aaaatctcca tagcgtttga
    16021 ctaatttatc aatttcgata atcatcatga aaaactcctt ccaaatttct tgttgatctg
    16081 actataataa agtgcaagaa aagaaactag tgacgattgg ctaaaccaca catgacattt
    16141 gtcacaatga aggagtctgc tatgcataaa tctcggctac tcaacgaaac tagctttcta
    16201 ctggttatct cattgctttt gctggtaagt caaggattcg ttgctatgca agttatctat
    16261 ttattgatcg ccgttttatt cactggtgtc agttatttac tgcctacaaa gccaaattgg
    16321 attttccaag ttattctatt tcttttatgt gcctttttac ctgatcttgt cctgtttctg
    16381 ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc ttgattactt gtttttgtta
    16441 ggttttttgt tgatttcgac ggttggtaat catcccccca tcaaggtcgc ctttgtcgta
    16501 attttgctgt ttttttcctt ttacttacga aaaaaagatc aacgaacgac cgatcttgag
    16561 aaaaagtaca ttgtctccaa agatactcat tgggaaaaac aacaagagtt atcggaacaa
    16621 aataaagcat tggccctgtc acaagaaaca caattagcct tgcaaattgt ggaagaacgg
    16681 aaccgaattg cccgagacat ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta
    16741 caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac taaaagaacc attagaaaat
    16801 ttaagtgcca ccgttcacac tgggatggat cggattcgtc aaagtgtcca tgacctccat
    16861 gagacatcga tctcttttca acactcctta gagtttctga tcgctgattt tccttcttct
    16921 gttacgattg aaggaaactt gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta
    16981 atggtcatca aagaagcctt aaccaacagt atcaaacata gtcatgccaa aaccgttgtt
    17041 ttacattttc gaacgttgcc ggccttttat cgcttgcaaa tcacgaatga tggcaccagc
    17101 ccacaaaaaa attctgctcc cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa
    17161 caaaagggac aagtccatat tttccaagaa aaccagcaat ttcagttaac gatcatttta
    17221 ccaagggagg gacaccaatg ataacagtag tcattattga tgatgatcca tttgtgacga
    17281 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
    17341 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
    17401 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
    17461 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
    17521 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
    17581 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
    17641 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
    17701 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt
    17761 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
    17821 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
    17881 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
    17941 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
    18001 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
    18061 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
    18121 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
    18181 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
    18241 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
    18301 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
    18361 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
    18421 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
    18481 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
    18541 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
    18601 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
    18661 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
    18721 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
    18781 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
    18841 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
    18901 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
    18961 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
    19021 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
    19081 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
    19141 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
    19201 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
    19261 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
    19321 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
    19381 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
    19441 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
    19501 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
    19561 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
    19621 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
    19681 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
    19741 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
    19801 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
    19861 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
    19921 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
    19981 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
    20041 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
    20101 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
    20161 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
    20221 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
    20281 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
    20341 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
    20401 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
    20461 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
    20521 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
    20581 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
    20641 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
    20701 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
    20761 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
    20821 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
    20881 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
    20941 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
    21001 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
    21061 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
    21121 cgaataaaag tgggccatct ttatgtcggg caaccgcatc tgctacaaca gtttcgatac
    21181 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
    21241 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
    21301 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
    21361 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
    21421 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
    21481 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
    21541 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
    21601 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
    21661 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    21721 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    21781 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    21841 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    21901 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    21961 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    22021 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    22081 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    22141 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    22201 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    22261 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    22321 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    22381 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    22441 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    22501 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    22561 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    22621 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    22681 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    22741 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    22801 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    22861 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    22921 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    22981 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    23041 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    23101 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    23161 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    23221 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    23281 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    23341 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    23401 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    23461 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    23521 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    23581 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    23641 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    23701 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    23761 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    23821 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    23881 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    23941 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    24001 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    24061 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    24121 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    24181 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    24241 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    24301 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
    24361 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
    24421 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
    24481 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
    24541 tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
    24601 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
    24661 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
    24721 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
    24781 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
    24841 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
    24901 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
    24961 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
    25021 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
    25081 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
    25141 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
    25201 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
    25261 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
    25321 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
    25381 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
    25441 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
    25501 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
    25561 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
    25621 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
    25681 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
    25741 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
    25801 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
    25861 aatccatgct ttgcaaaaag catcaagcca gatgtatccc gatctagtcg cgtcaccaca
    25921 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
    25981 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
    26041 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
    26101 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
    26161 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
    26221 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
    26281 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
    26341 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
    26401 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
    26461 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
    26521 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
    26581 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
    26641 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
    26701 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
    26761 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
    26821 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
    26881 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
    26941 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
    27001 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
    27061 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
    27121 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
    27181 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
    27241 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
    27301 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
    27361 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
    27421 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
    27481 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
    27541 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
    27601 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
    27661 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
    27721 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
    27781 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
    27841 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
    27901 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
    27961 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
    28021 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
    28081 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
    28141 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
    28201 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
    28261 taatccaaac cggaaagctt acaacaaaac gagctgaatt ccctattgaa gaaggacttt
    28321 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag
    28381 gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt
    28441 atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca
    28501 ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa
    28561 agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct
    28621 atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat
    28681 ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt
    28741 tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc
    28801 ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg
    28861 cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg
    28921 gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg
    28981 gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt
    29041 ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca
    29101 attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt
    29161 ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac
    29221 atttacatac aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta
    29281 attaaaaaag gctctttgtc aaatgatgtt ggtaaagaga aatcttagaa taatttggac
    29341 agaacagaaa gaagaaaacc tctttctgtt ctgtttattt ggttaaaaat gttttgccat
    29401 gacgaatcat aattcgtagt ttgaaattgt aaaagttgcg aaagccgtac gcattgcgtt
    29461 ttaatacttt gatgtggttg tttaagcatt ccaatggtcc gtttgaatag ctgtagtgaa
    29521 gcgcattttg gatctgtggc agaaactttt taaacgttcg taaagtggtt gtataggttt
    29581 ctggcagacc gctaatgtct ctagttaaac aatcctcaaa tagtgactga tctcttttct
    29641 ttaccgcata aagaatgtct tggtataact gatagccttg gcgtaagttg ggacaagcag
    29701 ctaacagtcg atcggcaatc tcactttcag tcaaataatc tttaaaactg gcacgccaaa
    29761 cccgtttttc atagtttaac tttgcttctt ctttttgtag gagtttccaa tagcgcttca
    29821 attttttccc taaccctcgt tgagagtggg aatttccttt aagtcgtacc gtggtttctt
    29881 tcactcgatg attcaagaaa gttctgccaa tatgttggac gatgtggaaa cgatcgatga
    29941 tcaactgagc attcgggaaa agctttttca ccaacgaaac ataaggcgca tacatatcaa
    30001 taacaatcaa ttgaacggct tcacgagtcg ctaatgaaaa ccgagaaaaa tagcgttcta
    30061 aa
//
LOCUS       DAZKNO010000010        29979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000010 DAZKNO010000000
VERSION     DAZKNO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.010"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..894)
                     /locus_tag="ACG8MY_000331"
     CDS             complement(<1..894)
                     /locus_tag="ACG8MY_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0429563.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGAS"
     gene            complement(1023..1787)
                     /locus_tag="ACG8MY_000332"
     CDS             complement(1023..1787)
                     /locus_tag="ACG8MY_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0429564.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(1791..2180)
                     /locus_tag="ACG8MY_000333"
     CDS             complement(1791..2180)
                     /locus_tag="ACG8MY_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429565.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            2315..2857
                     /locus_tag="ACG8MY_000334"
     CDS             2315..2857
                     /locus_tag="ACG8MY_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0429566.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            3005..3637
                     /locus_tag="ACG8MY_000335"
     CDS             3005..3637
                     /locus_tag="ACG8MY_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429567.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(3771..3896)
                     /locus_tag="ACG8MY_000336"
     CDS             complement(3771..3896)
                     /locus_tag="ACG8MY_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0429568.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(4030..4923)
                     /locus_tag="ACG8MY_000337"
     CDS             complement(4030..4923)
                     /locus_tag="ACG8MY_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0429569.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(5105..6031)
                     /locus_tag="ACG8MY_000338"
     CDS             complement(5105..6031)
                     /locus_tag="ACG8MY_000338"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0429570.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(6116..6877)
                     /gene="pflA"
                     /locus_tag="ACG8MY_000339"
     CDS             complement(6116..6877)
                     /gene="pflA"
                     /locus_tag="ACG8MY_000339"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0429571.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(7121..9352)
                     /gene="pflB"
                     /locus_tag="ACG8MY_000340"
     CDS             complement(7121..9352)
                     /gene="pflB"
                     /locus_tag="ACG8MY_000340"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0429572.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(9629..12079)
                     /gene="parC"
                     /locus_tag="ACG8MY_000341"
     CDS             complement(9629..12079)
                     /gene="parC"
                     /locus_tag="ACG8MY_000341"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0429573.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(12091..14136)
                     /gene="parE"
                     /locus_tag="ACG8MY_000342"
     CDS             complement(12091..14136)
                     /gene="parE"
                     /locus_tag="ACG8MY_000342"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0429574.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(14302..14736)
                     /locus_tag="ACG8MY_000343"
     CDS             complement(14302..14736)
                     /locus_tag="ACG8MY_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0429575.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            14863..15501
                     /gene="plsY"
                     /locus_tag="ACG8MY_000344"
     CDS             14863..15501
                     /gene="plsY"
                     /locus_tag="ACG8MY_000344"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0429576.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(15633..16508)
                     /locus_tag="ACG8MY_000345"
     CDS             complement(15633..16508)
                     /locus_tag="ACG8MY_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0429577.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(16590..17381)
                     /gene="codY"
                     /locus_tag="ACG8MY_000346"
     CDS             complement(16590..17381)
                     /gene="codY"
                     /locus_tag="ACG8MY_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0429578.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(17399..18799)
                     /gene="hslU"
                     /locus_tag="ACG8MY_000347"
     CDS             complement(17399..18799)
                     /gene="hslU"
                     /locus_tag="ACG8MY_000347"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0429579.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(18813..19361)
                     /gene="hslV"
                     /locus_tag="ACG8MY_000348"
     CDS             complement(18813..19361)
                     /gene="hslV"
                     /locus_tag="ACG8MY_000348"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0429580.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(19398..20303)
                     /gene="xerC"
                     /locus_tag="ACG8MY_000349"
     CDS             complement(19398..20303)
                     /gene="xerC"
                     /locus_tag="ACG8MY_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0429581.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(20501..21877)
                     /gene="trmFO"
                     /locus_tag="ACG8MY_000350"
     CDS             complement(20501..21877)
                     /gene="trmFO"
                     /locus_tag="ACG8MY_000350"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0429582.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(21846..23924)
                     /gene="topA"
                     /locus_tag="ACG8MY_000351"
     CDS             complement(21846..23924)
                     /gene="topA"
                     /locus_tag="ACG8MY_000351"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0429583.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(24046..24903)
                     /gene="dprA"
                     /locus_tag="ACG8MY_000352"
     CDS             complement(24046..24903)
                     /gene="dprA"
                     /locus_tag="ACG8MY_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0429584.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(24959..25726)
                     /locus_tag="ACG8MY_000353"
     CDS             complement(24959..25726)
                     /locus_tag="ACG8MY_000353"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0429585.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(25719..26579)
                     /gene="ylqF"
                     /locus_tag="ACG8MY_000354"
     CDS             complement(25719..26579)
                     /gene="ylqF"
                     /locus_tag="ACG8MY_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0429586.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            26839..27237
                     /locus_tag="ACG8MY_000355"
     CDS             26839..27237
                     /locus_tag="ACG8MY_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0429587.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            27474..28016
                     /locus_tag="ACG8MY_000356"
     CDS             27474..28016
                     /locus_tag="ACG8MY_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0429588.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(28099..28650)
                     /gene="lepB"
                     /locus_tag="ACG8MY_000357"
     CDS             complement(28099..28650)
                     /gene="lepB"
                     /locus_tag="ACG8MY_000357"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0429589.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(28802..29029)
                     /locus_tag="ACG8MY_000358"
     CDS             complement(28802..29029)
                     /locus_tag="ACG8MY_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0429590.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(29026..29544)
                     /gene="msrA"
                     /locus_tag="ACG8MY_000359"
     CDS             complement(29026..29544)
                     /gene="msrA"
                     /locus_tag="ACG8MY_000359"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0429591.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(29564..>29979)
                     /locus_tag="ACG8MY_000360"
     CDS             complement(29564..>29979)
                     /locus_tag="ACG8MY_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0429592.1"
                     /translation="LNGTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPI
                     KDVMNMIKRNYKLPDWIEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLY
                     LPKDTSDASNNSNTNKTSTSSTESKTKENKKSSDE"
BASE COUNT         8051 a         6314 c         5141 g        10473 t
ORIGIN      
        1 actcgcccct ttttcttcta ttttttttaa caacatttct ccacatactt cacccatacg
       61 tataatatct tgcttgatcg ttgtcaattt aggtgaactg atttggtcta aaaatacccc
      121 atcaaatcca atcacaccaa attcttcggg caagtgtcct ccagcattta taatccctct
      181 tactgctcct agtgctagcc gatctgaact acatacaaaa caagttttat ctgtaaaatc
      241 tgcccagttt tctgtcatat atctttctac ttctgtcgaa tgatttggaa accgcaagat
      301 tttactagga agatcaactt ccttcatcac agataaataa ccagtttccc ttgaaatttc
      361 aaaaggctcg tccacttcga taccgatata aataatcgtt tgataatctc tttctaaggc
      421 atattgcgtt gcttttgctg taccatattc attatcgctg tctacataat catatccttc
      481 agaattttcc ccaaacaaga caactggttt ttccaaacgg tcgatccaat cgaagtcttt
      541 ttttctgaca cctgtgatga tgtatccatc acaagaaccg acgtcgaagg catcatttgt
      601 aactaattgg agagaataat gcgctttacc gatccgttta gcgattccca tcagcaaatt
      661 catgtaatag ggctcagttg tgtcgatttc ttccagtata aataatttga tgatctgcga
      721 ccgattcgtg acaagtgctt ttgcagcaat atttggacgg taatccagtt cagccattgc
      781 tgagaaaact aactctttca attcgtcagt cacctgttcc ggatgattga tgactcgaga
      841 gaccgtcatt tttgaaacat tggcacgttt tgcaacatct gctaaagtgg gcatgctctc
      901 ttcctttcta acatgttgtc attcttacta tcaattatac caaattttcc taacagagtt
      961 tattatagcc caaaataaaa aacggaaaat cctctatcgt catgagtgat tttccgtttc
     1021 aattatttat acttttttat caaatttttt gctaaaaaag tggaagcttc ttccattagt
     1081 ggtatttgta ccatatgtcg ctgtgttttt gaggaacgaa aaacaatttc ttttccgtaa
     1141 gacttatgcc gatttttttc tacaaatcgt tccgtttgta tataagggat tttttcgtca
     1201 gcttgtccat gccaaaagaa cagaggacgt ccagctaact tttccggctg caatgacaaa
     1261 tcatagctct taacccatga aatcaaatca aaatagtctt ttggtacgac ataattatat
     1321 tccgatgcgc gccttacgat ttcttttcca tattccaaag gtgcagggga acccatcaaa
     1381 cacacgcccg ctactatatg aggatgttta gccaacaagg cgcatgttgt cattcccccc
     1441 atcgaaaccc cgccaactgc aagctgatca tgtagaagac ctcttttttc taaatgcttg
     1501 ataagtgtgt caaattcaaa cagatttccg tagatactat cccaaaatgt ataggatgga
     1561 atagaagaaa ccggctgttt gcgctcccca tgattcatcg catctggcag gatcacacga
     1621 atgccctgct gcgcaatttt tcttccttgc gtcaaaacta actccttact tgtctgccat
     1681 ccgtgataat agatgacaac gggaatacgt tcctctctta attctttcag aacaacttct
     1741 aacactggaa tatgatggac agaacgtttc aatataattg gattcaattg ttactcacct
     1801 attttcatta aatttcctga tcgccatagc aaccatgctc caacagttgt ctggaacagg
     1861 ttcagtgtac caaataatat aaatgcagga ctgaatggct cgggaaataa gcacagagaa
     1921 caaagtccca ccactcctaa tgctagcgaa aggagtggat aaatagaaaa attcaaaaaa
     1981 ctggcaacgc taccgaaaat attaccaata aacagtatca aaaagaaaaa aacgcttaaa
     2041 attaaaccat ctgtcagcga catctgaaaa acaatgttct ctacctcaat tggctgttga
     2101 atcgaataga cacctaagta tatactgaga aggagactta gcacgtggct ggcacaaaac
     2161 acacgattga ccgttttcat tttaatcacc taacctatct ttataattct attataatca
     2221 ttttatcatt tttttgtatg tataaagttt caatttttct tcacttacga ttttttttga
     2281 tattgttgtt atataaatga aaggagtttt acaaatgatt gatccaaaaa tgttggggat
     2341 gatcttcctc attaattttg cttatatcac tctgaacacg ttacgtttca tgttgacaat
     2401 gaaaggctat cgactgattg ctccacttgt cagtatggtc gagatcacga tttatatcgt
     2461 cggcttatcc cttgttttag atcgtttaga taatccactg aatttatttg tctatgcgtt
     2521 aggttacgca gtaggtatca gtgtcggaat tttgattgaa gacaaattgg cattaggata
     2581 catcatggtc acaacgattt tgccagcaaa tagcgcagaa gataaaagtt tgccgggtat
     2641 actgagagaa cacggttatg gtgtgacaca gacttctgca atggggctgg aaggtgagcg
     2701 gctagttctc gaaatacttt ctcctagaaa aagcgaacgt gaactgtatc gcctgatcaa
     2761 agaaatcgaa gagcgtgctt ttatcatttc ttatgagcca aaatatattt caggtggttt
     2821 ctggactaaa aaagtgagaa aacgacaagg aaaataactc acaaacttaa aaactgtcta
     2881 atatctaaat taaattttaa atgttagaca gtttttattt attattttga ttaaaaaaca
     2941 tcgacatata ctcatcaatt tgttattata ctttatatac ttatcaattt aatttggaga
     3001 tattatggaa attcaacgta gaaccactaa ttttttacat ttaggctctt tttttgcaac
     3061 tttgacgatc ttattttttc tatgttattc ataccttaag acctcacatg cgccttctag
     3121 tgcattaggc tggttgcttt tggatcattc tgatatcaca gaagccgaag aaaatccgtt
     3181 ttttttgatt ttgtcttctc tttgcggctt actgttttct gtttatttcg gtgcctattt
     3241 ttttcatcga catcctggta cgttaaacga agcttcaagc gtattgattg catttaaaac
     3301 gttttctttt ttagcaattc ttttttattc gtttaatcac aatacgcttt tcttctttat
     3361 catgaatggt ttagtcgtga ttttatccat ttttacttat tttatttttt cactcatttt
     3421 tagccaattg aaatgtccgc tcttctttcg attagttcct gtcacctgtt ttatttacag
     3481 cttgttttcc ttttttgtcc ttgctttaat gccactgacc aacccttcga cgatccaagt
     3541 cgcaggaaat ctgttggata tgctcttcat cgtcagtcta ggtgtattta tgtggcaaag
     3601 aaaaaacaaa caaaataaaa agcattttga agcctgacta ttacgacttc ataaatgctt
     3661 ttttagtttt aaacacttca atctaaaaga ataacccaag attggataaa ttattaatga
     3721 ctcgccattc gtttcttagt gattctggat tatcaaatgg acttcctaat ctaatcagct
     3781 agttcttccc ataaaatgcc atacttgact gaatcataat attgattatt taaaaatcta
     3841 acttttctta tcactacttc ctttgtcatt ccacatttct caccattttt ttgcattcct
     3901 ttgtttccag accatgtagt gaatctaata ggtggtagat aggcaaaata ctcaaataat
     3961 tcttttaacc ataaaggaag agaaaggctc ctcctgcaag agtcagtata atttttgctt
     4021 actgctctct taggttttac tcactaaaac tgttccatat tctttgatat aagaaaatgc
     4081 atgcggtata aatttcttgc tcagctcaga aaagatttgt cgtttaagaa aggccatttc
     4141 ctccattgtc aatgaaggat ccaatgttgg taattcaatc tcaaaattgt ttttttcgac
     4201 aataggtaat tctaatgtga atgattcttc ttcctttata gtattcgtca caagtagaga
     4261 ctgatggaaa atactaggcc ggcgattttt atatttattc ttacgtatcc tgtcaacgat
     4321 aacttctatt tgatcaaaaa agtacgattc atttacgtct ccaataagtt cggctaattc
     4381 tttgaatgcc ttcgttacag gaagctgatt cttctgtaca aaaaagtaat tagccaaagt
     4441 aagcgggaag tgttctatag aggaaagtgt gataaaacgg tacttctcat ttcctgcagt
     4501 tgtcctcagc aattctgtct ctcggctgtt tcttactcca taaaaatacg cttctttccg
     4561 ttcatcttcg aaacaatacg cccaaataac ttctcccatc gctaattttt gttcatcaac
     4621 agagagtctc ttcaattgtt cagctatggt ttcagcagcc gaagtggctt gttgcaagta
     4681 atctttagga tcaaatattg gaaagttcaa atgatatctt cgttcttccc tcttgattaa
     4741 tcctgcatcg atcaataaat caagaaaatg ggaaaattta ttttgcgaaa attctttttt
     4801 caattctctt agaatcgttt cttgttctcg atgttcatat agataatcgg tcaatgaaga
     4861 aaaaagcgga tcatcggtca gtttttgata aaagttttcc ttattttttg aataatagtt
     4921 catatatgtt ctcttttcta tccgttttat tttacttaaa aggcttttac gtaaaaagga
     4981 ggaagaacaa gagtcataat tgactgttat tctcctccat ttcttcggcc tttagtatgt
     5041 tctatgtttc tttttatatc aatcgcttat ccattcattt tgaatgcttc atactaccat
     5101 ttgattagct aaatacagcc gtcaattgag gtacgacttg ttttttacgt gaaacgacac
     5161 ctttaagcaa agcacggttg ttggtcaaag ttgtttggaa agcttcttct actttttctt
     5221 ttcctttacc agcaacaagt aattctgaat cactgtctaa gatgtttgtg acaactaata
     5281 cgaacagatc gtagccattt gctttcattt catcttccat tgctgcttct agctctgctt
     5341 gtcgatccat cacttcatta aggtccactg tattgacttg agcgatacga acgttggaac
     5401 tgcccattgg gaaacttttt gcatccaaat caagaagaac cgcagctgat ttatcactta
     5461 gatttgttcc tgctttcaac atttcaagtc cataagtgtt tagatcgact tcagcgattg
     5521 ctgcaagttc ttttgcagct gcttcatctt caggcgtaca tgttggtgat ttgaacaaca
     5581 atgtatctga aatgatagct gagagcatga ttcctgcgat ttctttagga attgttactt
     5641 ggttttcttt atatagtttt aaaataatcg tacttgtaca tccaactggt tctgcacgat
     5701 agtacaatgg attagctgtt tcaaagtttg caatacgatg gtggtctact acagctaaaa
     5761 tatttacttc gctgatatct gaaatacttt gttggaattc attatgatcc acaagcatga
     5821 catctgcatt ttctgcttta gctgactcta ctactcgtgg tgcagatagc tgaaagtggt
     5881 ctaatgcgta ttgtgtttct tcattaggtg tacctagagc aactgcttct gtttctttac
     5941 ctaattcatt ttgcaagtgt gaaaacgcaa tggctgcccc gattgcatct gtatctggat
     6001 tttggtgacc aaaaactaaa atttttgaca tgtcattcgc tcctttgtga tttctcctat
     6061 ttaataatag caaaaaaacg gaaagaagca atacttatct tctttccgat gcgacctatc
     6121 gcgttaaata ttttgtatac tcttctgtat gaagcaatcg tttagcattt tcgacacgtt
     6181 cttttgtagg tggttcgatc ccttctaatg gatatttcaa accaagtgtt tcccacttgt
     6241 attttcccat cgtatgataa ggaagaatct ctactctgtc tacatttgac aaggtttgaa
     6301 taaagttgtc tagtcggatc aaatattcat cataatcact tcgctcagga actagtacat
     6361 ggcgaatcca aaccggtttg ttgatttttg acaaatattg agccatttct aaaatatttt
     6421 cattgtccca atgcgttagt tttttatgct cttcattatc gatgtgtttg atgtcaaata
     6481 acagtaaatc tgtatacttc atcaattctt gaaaacgtga aaagaaaggt tcttcatacg
     6541 taaaaggttt tccgcatgta tccaatgtag tatgcatctt ccgctctttt gcttttttaa
     6601 atagttcgat caaaaaatcg atctgcaata gaggttctcc accactaaca gtaataccac
     6661 ctttatctcc ccagtaaggt ctgaagcgtt ctgctttatc taaaagttca tctgctgtgt
     6721 attctttccc tccgccaata ttccaagtat ctggattgtg acaaaattgg caacgcatgc
     6781 ggcatccttg cataaacacg acaaagcgca gtccaggtcc gtctactgaa ccgaaagttt
     6841 cgatagaatg aacatagccg attgtttttt cttccattta agttcacatc ttttctttga
     6901 tttcatttta agtacaagtt agctgtcgaa gcgaagcttt ctgaacaaaa tttttcgcaa
     6961 ttgccgcttc gttatgtgta aaatttacag ggacggaaca tacaagtcct gcccctgtaa
     7021 atcgtttctt tcaaataagc atactgatta ttcgattttg atgcgatggc tattcaacgt
     7081 cctttcgacg ctaagtcgaa aaactttttt cacgaacttt ttacattctg tcgtgtgaag
     7141 ttctcatcaa gacatctaat tgttgttctc ttgtcaagtc acggaattta actgcataac
     7201 cagatacccg gatcgtcaag ttcggatatt tcattggatt ttccattgca tcacgcaata
     7261 aatcattact gaatacattg acattcaaat gataagctcc ttttgagaag tatccatcta
     7321 agacattacg caagttttcg attcttgtat cttgttcttt tccaagacct gaaggattga
     7381 ttgtttgggt atttgaaatt ccatccaatg cacagccgta attcatttta gctgttgagt
     7441 tcaggctagc taataaccca ctttgttccg cattgtaact tggatttgct cctggcgcaa
     7501 gtggttttcc agcttcacgg ccatcaggag tatttcctgt atttttaccg taaacaacat
     7561 ttgaagtaat cgtcaataat gaagttgttg gttttgagtt gcggtaagtt ttatgacgtt
     7621 tgatttgtcc catgaagtat tccaagatcc atgaaactaa ttcatctgca cggtcatcat
     7681 catttccgta tttagggaag tcgccatcca cttcaaaatc aactgctatt cctttttcat
     7741 tacggattgc ttttactttt gcatatttga tcgctgacaa gctgtctgcc gcatgagaga
     7801 tcccagcaat acctgtagca aatgtgcgtt gcaagtcaga atccatcaaa gccagctgag
     7861 ctgcttcata agaatattta tcatgcatat aatgaatgat gttcaacgta ttgacatata
     7921 gttctgctaa ccaatccatc atattcatga agttatgttt cacttcttca aagtctagta
     7981 tttctgatgt gattggacga tattctggtc caacttgggc gccagtcact tcatcgactc
     8041 cgccattgat ggcatataat agtgttttag ccaagttagc acgagcgcca aagaattgca
     8101 tgtcttttcc gacaactgtt cctgatacac agcaagcaat cgcacaatca tcagaacccc
     8161 attgttcacg aagctgatta tcattttcga attgaatcga agaactttcg atagcgattc
     8221 tgctagcata tgttctgaat ccttctggaa ggtgttcaga atacaaaaca gtcaagtttg
     8281 gttctggtga aggtcccata tttgttaatg tatgaagcag acggaaatca ttctttgtaa
     8341 ctaatgaacg accatctaat cccatacctg caatggataa agtagcccag atagggaatc
     8401 ctgagaataa ttggttatat tcaggtgtac gcgcgaattt gaccatacgt aatttcatta
     8461 ctaagtggtc gatcaattct tgtgcttctt tttctgtgat tactccatta tccaaatcac
     8521 gttggatata gatatcaagg aatgctgaga tacgtccgat tgacatcgca gcaccatttt
     8581 gtgatttgat tgctgctaaa tatccgaagt atagccattg gacagcttct tttgcatttg
     8641 cagctggttt agaaatatca tagccatatt tcgcagccat ttctttcatt tgcgctaatg
     8701 cacgatattg ttcagtgatt tcttcacgta aacgaatcac ttcttcactc attgtatgat
     8761 aaccagtcat atcatgatct tttttctttt gttccatcaa atagtcgatt ccatacaatg
     8821 ccacacgacg atagtcaccg atgatacgtc cgcgaccgta agcgtctgga agccctgtaa
     8881 tgattttgtt tttacgagca gcacgcattt cagcagtata agcgtcaaat acaccttgat
     8941 tatgtgtttt gcgccagtca gtgaaaatat gtgtcaattc atcgtttggt gtatagccat
     9001 tgctttttag cgcattgtta gccatattga ttccgccgaa cggcatgaat gcttgtttca
     9061 aaggtttgtc tgtctgtaga ccgacgattt tttccaaatc tttatccaaa tagcctgcat
     9121 cgtgtgaagt aatagttgat acgatttctg tgtccatatc atagacaccg ttacgctcga
     9181 attggatatc atttaacttt tgcaattcat cccataattt ttctgttgct tctgttgggc
     9241 cttccaaaaa gctgtcatca ccgcgatatt cagtgtagtt attttgaata aagtctctag
     9301 tgtctacgcc ttctttccat ttggtacctt taaatccatt ccattgctcc attgtatgga
     9361 cctcctaaat ttctgtttta tgtaacagtt tttacgtgat tagtataaca cagcacccta
     9421 tctttgcaag ggttttcttg ttttttattt aacaattttt atgttttatt tacatatatt
     9481 taaatcaacg ttgacatacc aacatttaag cattgtgaac actttcatct ttttttgcaa
     9541 acgtataagg atttattaaa aatgaatgaa aaagagacgc cacctgtatt aatacagatg
     9601 gcgtctcttc attaaaatac tacaacagtt acgactcgat gaaaatctca ggagcatcga
     9661 taacttcgat gacttctcca ccttttttct catccatcgc aaatgatcca ttcgatgttc
     9721 gatcacctat agggaaatca gttgggtcaa tcgttaactg ctgccctttt tgattcatca
     9781 aggttatttc ttttgatctt ccttccgaca tatagataat acggtgcgga ttttttttca
     9841 gctcacgcat gaccgtcact ccccgcttcg cacgtgaagt ttggctgatt tcttgagcaa
     9901 gcatgcgttt aacagctcct cgctgagtaa cgattacgat cggcgtatca ccttctgcta
     9961 acacaagcag cccgttgata atcctatctc catctttcaa attcatagac ttcactccag
    10021 ctgcctttgc tccaacaacg ggaacatctg ttaaaggata gcgcaaaccc attccttgat
    10081 gcgttactaa gaaaacatct aagcctggct gatcttcttt cagatacaca gcaatcagtt
    10141 catcttcttc cgcctttagc ttcatacagc tgagcggacg gctcttatag gtgcgccatg
    10201 gttcgaactc tgtcattcgt gtttgcttga tcaatccatt cttgctgatg aatacaaatg
    10261 ttttctctgc ttgaattttt tgatatggga aaacagctaa aatcgattcg tctacagaca
    10321 gattggagat tgtttgagaa atgtgctcac cagcatcttt ccatttcaaa tcgggtagtt
    10381 catggacagg gcggtagatc acatttgctt tatttgtgat cagaagaaca tggtccaatg
    10441 tactcaattc acctgagtat agcaagtagt cgccttctcg catcccgatt tcttctggtt
    10501 tggaagcact ataagaacga aggcttgttc gcttcacata gccttctcgc gtcacactga
    10561 caattacgtc ttcttgtgcc actaagactt gcgtatcaat tttgatttct tcgatttcgt
    10621 cttcgattgt cgttagacga gcggaagaat aatttttttt cacttctcgc aattcttttt
    10681 tcataacaga gaaaagctct ttgtcatttg aaaggatttt attcaactct gtgatttgcg
    10741 caatcaagct ttcggattcc ttttgaagct ctgtgatatc cgtgttcgtc aaacggtata
    10801 attgcaatgt gacaattgct tctgcttgtt cttctgtaaa ttgaaagacg tcgactaaat
    10861 tctttttggc gtcttttttg tctttacttt cacgaatcgt tgcgattact tcatccagaa
    10921 tcgataaagc tttcatcaat ccttctacga tatgctgtct tttttgagct ttggctaaat
    10981 cgaattgact acgttttgtt atgacttgct ttcgatgctc gatataactt cttaaaatgt
    11041 ctttcagtcc aacttgatgc ggagtcatat ggtcgatagc gaccatattg aaattgtaat
    11101 tgatctgtag ctcagtgttt ttgaacaaat aattcaaaat tccttgagca ttggcgtctt
    11161 ttttcaattc gacgacaatt tgcagacctg ttctatcact ttcatctcgt acttcagcta
    11221 ttccatcgat tttcttattc aaacggatct catccatttt tttcaccaag gtcgctttat
    11281 ttacttcata tgggatttct gtgatcacga tttgctgttt attacccttc attgcctcta
    11341 tctttgtttt cgaacgaaga ataacttttc ctcgtcctgt ttcataagct tttttgattt
    11401 catcttttcc ttgcaagatc cctcctgttg gaaagtcagg tccaggaata tattccatca
    11461 atttttcaag gctcgcttgt ggatgatcga tcatatagat tgtgccatcg atcacttcag
    11521 ctaagttatg agtcgggatt tctgttgcat atccagcaga tatacctgtc gatccattga
    11581 cgagaaggtt tgggtatcga gctggaagga ctgttggttc tttttctgta tcatcaaaat
    11641 tccacacgag atcgactgtt tctttctcga tatctgctag catctctcca cttaacttgg
    11701 ataatctggc ttcagtataa cgcatcgcag caggtggatc accgtccata ctcccgttat
    11761 tcccgtgcat ttcgatcagt acttcccgca acttccagtc ttggctcatg cggaccatcg
    11821 cttcataaat actgatgtca ccatgaggat gataattccc catgatattt ccgacagatt
    11881 ttgctgattt acggaatcct ttgtcatacg tattgccatc tttgttcatg gcaaataata
    11941 tccggcgttg cactggcttc aatccatcac ggatatcagg cagtgcacgt tcttggataa
    12001 tatatttgga ataacgtccg aagcggtcac ccatcacttc ttctaaagtt aattcttgta
    12061 cttcttgtcg attttccatc aggtgacctc ctattctact tcaaataact gattgttttc
    12121 atttttatct gctcgttctt catctaataa atcattagaa actgaaggag agatttcagt
    12181 atcctctttt ttatctaaga tactgccatc ttcttcgagc gtaaattgca cgtgattttc
    12241 gatccatttt cttcgaggtt ctactttgtc acccatcagt gtggttactc gacgttcagc
    12301 ttgcgcagca tcatcgatac ggacgcggat caacgtacgg gaagtcggat ccatcgttgt
    12361 ttcccacaat tgttccgcat tcatctctcc aagtccttta tatcgctgca gcatatatcc
    12421 tttgccgact tttttaatca ttgctgccaa ttcgtcatct gtccacgcat attcaatgac
    12481 ttgcttcttt ccttgtcctt tggatacttt atataaaggc ggtaacgcga tatatacttt
    12541 cccagcttca atcagcggtt tcatataacg ataaaagaaa gtaagcagta atacttgaat
    12601 atgggcgccg tctgtatccg catcggtcat gatgattact ttgtcgtaat tacagtcttc
    12661 gatagaaaac tcaggaccga ctcctgcacc aatcgtatag atcatcgtat tgatttcttc
    12721 atttttcaag atatcttgca ttttagcttt ttcggtattg atgacttttc ctcgtaaagg
    12781 tagaattgct tggaactttc tgtctcttcc ttgtttagca gatccgccag cagaatcccc
    12841 ttcgactaaa tacaattcat ttttcttagg gtttcgggat tgtgcgggtg tcaattttcc
    12901 tgaaagaaga gattcgcctt ttttacgctt cttcccattt cggctttctt ctctggcttt
    12961 acgtgctgct tctctcgctt ctctggcttt gattgcttta cggaccaaca tctggctcat
    13021 ttcgctattt tcctgcaaat aaaagcccat ttgttcactg atgacgttat ctactacggt
    13081 acgagcaact ggcgtaccaa gtttttcttt tgtttgccct tcaaattgca gaagattttc
    13141 aggtacacgg atagaaagta cagcagctaa cccttctctg aaatcactac cttccagatt
    13201 cttgtcccgt tctttcagca aaccaacttt tctcgcatat tcattataag atttcgtcat
    13261 cgccgctttc ataccagctt catgggttcc cccatctttt gtccgaacgt tattgacaaa
    13321 agataaaaca ttttctgaat aaccgtcatt gtattgataa gcgacctcta cttcgatacc
    13381 ttctttttct cctgaaaaat aaacaactgg tgtcaatgta tctttttctt cattcagata
    13441 atcaacaaat tctttgattc cttcttcgta atgaaaaatt tccttgactg gttcttctcc
    13501 acgaagatca gaaagagaga ttttaacgcc ttttagaaga aaggcagatt ctcttaaccg
    13561 ttcggctaaa atttcatatg aaaatttcgt tgtactgaat attgtatcat ctggtaaaaa
    13621 atggacagtc gtcccgtttg ctttttttgt ttttcctatc tttttcaaag taccgtcagg
    13681 tttaccgccg tttttaaatt tctgctgata ctctacgcca tctctaacga tcgttacaat
    13741 tagccatttc gataaggcat ttaccacact ggctcctaca ccgtgcagtc caccagaagt
    13801 tttgtaccct ccttgaccaa actttccgcc agcatgcagc acagtaaaaa tcacttcaac
    13861 tgttggtatt ccggaagcat gcattcctac aggcatccca cgtccgctat ctgcaacagt
    13921 tatactattg tcttcatgga tggtcacatc aatctcactg ccataacctg ataatgcttc
    13981 gtcaactgca ttatcgacga tttcatagac taaatgatgc aagcctcggc tgtcagtgga
    14041 tccaatgtac atccctggtc tttttctaac tgcctctaat ccttccaata cttggatgga
    14101 cgagtcattg tattcgttgt ttactttttt tgccaaggca aacgctcctt caataatact
    14161 ttgcggcaaa accgcaccac gattatcata ctagaaaagg tcgtgaaaaa aaagagggta
    14221 gacgagaaat caattttaat tatctcctaa ccgctttttc gacattcccc tctatcgatc
    14281 atctcttctt tgtggtcatt tttatttttg gttttctttc aacttaccaa gttcaatctt
    14341 aatacaacga tccattacga tatctgtacg tcctgctttt cttagcatct cagctgcttc
    14401 tttgttttca atccctaatt gtgcccagaa cactttagca tcagttttca aaaaatcacc
    14461 tgctatttca ggtaaaaatt cactccgtct aaatacatct acgatatcga ttggatcatc
    14521 gatttctgtc aaattcgcat agactttctc gcctagaatt tcttcaccag ccaataatgg
    14581 atttactggt atgattttat atccattgtc ttgcaatacc tgtgcaacca tgtaacttgt
    14641 tcgttctggt ttattgctca agcctacgac ggcaatattt tttgcttctt ccaaatagtt
    14701 gaaaatcgtt tcttgtgttg gattttttac agacatttct gccacccctt gcttttcttt
    14761 ttattccatc tattatactt gtatctttca ttttttcaat tgttcctgtt acctattcat
    14821 gctaaaataa aggatagttt tcaacttgaa agagggtaaa aaatgaaatt gatcttatta
    14881 cttattttag catatctttt aggttctatt ccttccggtg tctggattgg caaattgttt
    14941 ttcaaaaaag atatccgaca acacgggagc ggtaatactg gaacgaccaa tacatttcgt
    15001 gtattaggca aaacagccgg gattgttgtc ctgcttatgg atattttaaa aggtacattg
    15061 gctacttgtt tgccgatgat cttccacctt tctgggatta accctctttg gtttggggta
    15121 tgcgccattc ttggccatac atttccgatt tttgctgggt tcaaaggagg aaaagctgtt
    15181 gcgacaagtg caggaatgct tttaggattt aatcctgtat tttttgttta ttcttgtatc
    15241 atctttatcg ttagtttatt ttgtacaagt atggtgagtt tgagcagtat gatcagtgca
    15301 gtattgatca ctttatctac gatcgtcctt ccttatgtag ctcctgtgat tttggctaag
    15361 cccaattggt tactgacgat cattgccttt ttagtttctt cctttatctt ttatcggcat
    15421 aaagacaata tcaagcgtat cagaaaccat actgaaagtc gaatcccgtt tggtttgaat
    15481 agcagcaaga aaaaatcctg atactttttc attccaaaga gataaaacgg gagtgtgaca
    15541 aatcgatgtc acactccctt attacttaca ggatagcttt tttacacacc ttggtaaacg
    15601 gtgttccctg ctaacttctc ggtcacaacc tcttacttca ctgtgatgga atattttgtc
    15661 ttgaagattt catgggcacc tagttgattg atccctactt tttccatcaa gtttccacta
    15721 ctgttcaccg tgtcagcgat tccgcaccac ggttcgatac aaacgaaagg cgcttctttt
    15781 ggataaggag accagatccc tacataaggc atatctttat agcttaaagt tacgctatgg
    15841 gaagattttt cacttcgaat ggcgtacgca tttagacccc ttgtttcaaa aaccaaggca
    15901 tcttgctcaa aaagttcacg attcaaagcc agattcgtat ttgtctgacc taacgtcttt
    15961 tgatccatat cgatgaatgg gcctttcaat ggtaggcgta atcttgattt caatggagaa
    16021 aatgccagat agaaatcttc aaatgacaat ccttcttcaa gaggtacatt gaaagcagga
    16081 tgtccaccga tagaaaagaa catttctttt tcaccggtat ttgccacttg ataatgaacc
    16141 gtgatgccgt ctcctcctaa ttcatagctg atcactaatt caaaatcaaa tggatacttt
    16201 tctaatgtct ccggcgtact ccttaagatg aaactagcca tttcttgtcc atgttcaatg
    16261 acttcaaaat ccatatcccg agcaaatccg tgttgcccca tttgatatgt tttcccttga
    16321 taaacatact gatcatcttt taaacgtcca acaaaaggga ataaaaccgg tgcatgtctg
    16381 ccccaatacg ctggatcacc ttgccagata tactctaaac catgttcttt tgatttcaga
    16441 ctgatcaatt ccgcaccgtg ttcagcgatt tcggcgatca gctggtcatt ttgaataata
    16501 acagtcattg ccattcctcc aacttcgact tactttttaa aatgggaagg ctggaacaaa
    16561 catttctatc tgccccagtc cccttacttc taataactgt gttttgctaa ctcgtcttta
    16621 aaacgggaat tcaatacttt taaataagtt cccttcatgc ctaacgaacg agattcaatg
    16681 attccagctg attccaattt tcgtaaagca ttgacgatca cagaacgagt gatgccgata
    16741 ctatcggcaa tattagaagc agtcagacga ccttcatctc catccaatgc ttcaaagata
    16801 gcttgtacag ccttcaattc gctataagat aatgtgttga ttgccatttg gacagccgtt
    16861 gcactccgta catctgcttc gatgcttcga gacttctgat aaaggatctg cataccgaca
    16921 accgtagctc catattctgc taagactaga tcgtcatctg caaacgattg ttccatacga
    16981 gataagataa tcgtacccaa acgttcgcca gctccaaaga tcggaacgac agtcgtcaga
    17041 ccaaatggat atttctctct tgactctacc ggaaatgcag taatatcact agtaattggg
    17101 atattagctt cagttttcat caactgtgcc actgtatcag tataagattc aggaaattgt
    17161 ttctcaacaa acattgtttt gattcgttca ttgttcacat cgtgacgttc attataacct
    17221 agtaatactc cgtcacttcc tatgatatag gtgttgctgt caagaatacc acctagtgtg
    17281 acagccattt tatcgtaagg caaatctgct tgcatgtcaa aagtattctt ttgctgcaaa
    17341 agtgtgttta tcttgcgggt tttcgttagc aagtctgtca ttgtcgtcct ccgatttttt
    17401 ataaaatata gcgacttaaa tcttcatttg ccgcaattga gccaagtttg tcatccacat
    17461 atgcttctgt gatcgttatt tctcccattt gcatatctgg tgcttcaaat aatagatctt
    17521 ctagtagttt ttccaaaatg gtatgcagtc tgcgtgcgcc gatattatct gtgtcacggt
    17581 tgacatcgaa agcgatttga gcgatccgtt caattgcttc ttttgtaaac gtaactgaca
    17641 cattttctgt accgatcaac gcgacgtatt gttttactaa agcattattt ggttcggtca
    17701 gaatcttcac aaaatcttca gctgtcaaat catctaattc tacccgtatc gggaagcgtc
    17761 cttgaagttc tggaattaga tcacttggtt tagacaaatg gaaagctcct gaagcaatga
    17821 ataggacgtg atctgtttgg atgatcccgt attttgtgtt tacttgtgaa ccttcgacaa
    17881 ttggcaatat gtctctttgg acgccttctc tggatacttc tccagattgc tgtgatttag
    17941 aagtgatctt atcgatttca tcgataaaaa taattccact ttcttccgct aatttgattg
    18001 cctcactatg gatatcagca tccttgacta attttgctga ttcttccttg atcaataact
    18061 ctaaagcttc tttgactgtt accgtccgtt ccactttttt cttcggtgtc aaagcaccta
    18121 gagtttcatt taggtcgatc cccatttgct ccaatccatt gttcatcatt ggtgcaggtt
    18181 tcttttggtc ttcaacttca atggttactt cacgactatc cagtaaaccc ttttgtagtt
    18241 gttccaagac tgtacggcga ttgattcgaa tatcttctgt cacttcttct tgcggttcag
    18301 cggtattttg ctgactagca ttaaaaagat tcatcatttg ttcaaattga ttagtggatt
    18361 gtttctgttc ttttttgata ccaggtacca agatttttac caaacgtttt tctgcatttt
    18421 ttttagcact tgtgtataca cggctgtact gctctttttc tacgatttgg atcgcatttt
    18481 ctactaaatc ccgaaccatt gattctacat ctcttccgac ataaccgact tcagtaaatt
    18541 ttgttgcttc gactttgaca aatggtgcat tgacgatttt tgccaaacgt cgtgcgattt
    18601 ctgttttacc gacccctgta ggtccgatca tcagcatatt ttttggtgta atatcttgtt
    18661 gcattttttc atctaactgc aaacggcgat agcggttacg caaagcaacg gcaacagatt
    18721 tttttgctgc gtgctgtcct acgatatatt catctaattc acttacgatt tgtcttggtg
    18781 tttgttttaa ttcatacata tctactcacc tcttacagtt cttctacgat aatattatga
    18841 ttagtaaaga tacagatatc tgcggcaata tttaacgcat tttctgcaat ttctttcgca
    18901 ctcatgtcgt tttgagcata gtttttcatt gctcgggctg cagccaacgc aaaattccct
    18961 cctgatccga tagctaagat accatcatct ggtgtgataa cttctcctgt acctgaaaca
    19021 agaagcattt ctttatcatt cattacgatc agcatcgctt caagtttttg catagattgc
    19081 tgtgtccgcc attcttgagc taattcgaca gctgcgcgtt gtagattccc gttatattcg
    19141 tttagtttgc cttcaaattt ttcttctaaa gtaaacgcat ctgcaacact tccggcgaaa
    19201 cctacaacaa cttctccatt gtagatacgt cgtactttct ttgcagtgcc tttcatgaca
    19261 acagattcac ccattgttac ttggccatct ccagccatag caaatttccc gtctttttcg
    19321 acggcacaga tagtggttga atgaaatggt gattcgacca tgtttgtttc ctcctaaatt
    19381 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
    19441 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
    19501 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
    19561 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
    19621 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
    19681 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
    19741 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
    19801 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
    19861 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
    19921 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
    19981 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
    20041 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
    20101 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
    20161 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
    20221 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
    20281 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
    20341 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
    20401 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
    20461 agagcgagac tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag
    20521 ttttttctaa ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt
    20581 catatcttgc ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg
    20641 cattcatcgg ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa
    20701 tagctgtttc ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga
    20761 ttccagcaag tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac
    20821 ctgcaaagaa cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg
    20881 aattcataaa gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc
    20941 caggaatcat ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca
    21001 cgatgttgta taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac
    21061 gtttgccagt tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt
    21121 tgaatcctcg ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct
    21181 caaaagattt aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt
    21241 cctcttctgt catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca
    21301 aatatacttt atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga
    21361 aataaaaacc atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca
    21421 aaggtcctgt tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca
    21481 ctgttactaa tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt
    21541 caacagctaa ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg
    21601 aattcaatcg gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc
    21661 ttaatgaatt ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt
    21721 tgactggacg catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag
    21781 cttcacttcc ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt
    21841 ttctattatt tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg
    21901 cctttgactt tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt
    21961 ttatcccaag aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt
    22021 ttcttagatt ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt
    22081 tcttttacga ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc
    22141 ccatatttcc ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct
    22201 ggttcatctt tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca
    22261 aacggtttat agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta
    22321 tccaaatctc cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca
    22381 cagattaatg aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat
    22441 tttacatagt agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct
    22501 accccgtttt cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga
    22561 gtaaaatgtt gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct
    22621 ggaagaatat tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg
    22681 aatcctttga atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat
    22741 gtcactttca ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag
    22801 atcaatgtat aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta
    22861 cgaagcacac tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg
    22921 atctttcggt gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg
    22981 gcctctgctt ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag
    23041 ccgactgttc cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg
    23101 gtttttcttg ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg
    23161 aaaggattcg cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct
    23221 gttccatcaa ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc
    23281 ttaccgtcga ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg
    23341 tcgatgctcc aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata
    23401 atttttaaag cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag
    23461 agaataggac tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca
    23521 tctactaaat ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt
    23581 gtgatttcat tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct
    23641 aaatgccaag caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga
    23701 gctttttttg ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata
    23761 taatggggtt cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt
    23821 atatggccaa cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg
    23881 gcttttgcag gtgattctac aatcactaaa tatttatacg ccattactga atggactcct
    23941 ttccattgtc gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc
    24001 cattcagaaa acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc
    24061 tgccaagata tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca
    24121 tccggtagaa tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc
    24181 caatgcttgc tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt
    24241 tcctttagac aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc
    24301 ggattgagga ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc
    24361 acgattttca gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc
    24421 accatgagag attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac
    24481 gatcacatat ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa
    24541 agcagaagtg tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc
    24601 accattataa aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt
    24661 gggatcaaag caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat
    24721 ttcctgccaa cttttgcgaa acagctcccg gtgtttggtt attttaccta tcttaatgat
    24781 gtcttcaaat ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt
    24841 tccttgacta ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata
    24901 catcataaaa ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt
    24961 attgtaaata tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc
    25021 gttcaagtcc ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc
    25081 caggataaag cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac
    25141 ttgcagcagc aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag
    25201 gcaaatccaa ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta
    25261 ctgcttggat catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct
    25321 gatcaacgat cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt
    25381 ctcgcttttt ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg
    25441 gcaagataac tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa
    25501 tgccggcaat ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt
    25561 catatttttc cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt
    25621 gctgcacgcc tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct
    25681 gtatgctttt caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc
    25741 atttcttcgc aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg
    25801 actaattcgc tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc
    25861 agtaattccg gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt
    25921 cttcctgaat aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg
    25981 acaagctggt ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca
    26041 aatttgggcc ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc
    26101 cactgctgac cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact
    26161 agtcgattca tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata
    26221 gcgcgaggct tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta
    26281 gccgcaggta ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga
    26341 tggccttgtt tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc
    26401 gctttattca ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta
    26461 cgagaagata aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt
    26521 ttttcagaaa cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt
    26581 gttctctcca ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc
    26641 aatataaatt cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac
    26701 cagtcctcat tctatcacat cttcactttt ttgatgatgc aaatttttat tttggtttct
    26761 atatatgaat ctaaattttt gacagcgttt cctaaataag agatgataga cgaacaaaac
    26821 agaaaggaga cgaaacaaat gaatagtatg gtttttgtta attttcctgt tgctgatgtc
    26881 aatcgttccg ttgcttttta tgaaaagctg ggttttaaaa aaaatgaaga attttctact
    26941 gaagaagctg gttcaatggt atgggatgac actttttgga taatgcttct ctctcatgat
    27001 ttttataaaa aatttataaa agataaagaa atagctgata ataaaaaaac aagtggtgct
    27061 ttgatttcgt ttagtgttga aaatgcggat gaagtaaaaa aatttgctca aattgcaaaa
    27121 gaaaatggtg gtactttcca cgaagtagat atgggaatgc ctgaagaaga aatgttttca
    27181 ttagaagtcc aagatctaga cggaaataca ttagaacccg tatggatgaa gatgtaagca
    27241 gataactaat ctgacgtttt ttataacaaa aaaacaaagg cggatgatac gataagtatt
    27301 ctccgcctct atttttttta ttcgtacaat ctctttttta cagtttataa aagcagtctt
    27361 ctcacccttt ttttaattgt tagatgccag tgtatgtaaa tcaccaatgt gtcatgacac
    27421 attggtgatt ttatggtatc cttttattat taactgacaa aggagagaat attttgaaca
    27481 ctttgattgc aaaagacgtt tttagattgc ccgaaatgaa tgcaagattg ttgtcgacct
    27541 ctgctctttt attagccctt gatgtcatct tatacaagat agcgattggt cctgcttatt
    27601 ttcaaatcac attcggattt ttaagtatgg cgcttatggg ttatctatat ggaccgatct
    27661 gggctggttt gcttgaaatg ttgtcgaaca ctttaaattt cacgatattt ggttcaggaa
    27721 cgtttcaagc tgcttttctt atcacggctt tccttggtgg atttattaat ggcttatttt
    27781 tatatcataa aaagataaac ctgccttccg tcatcgctgc ccaattattg attatgatcc
    27841 cagtcagcct ttttatcaat tcttggctga tgtctgtttt gtttggtgca gatttcaaag
    27901 taatttttct aggtagattc atcaggaata tgatccaaat ccctattcaa ataatggttg
    27961 tttattcatt tatcaaaact ttagaggcta gaaaagtgtt taaacgagct gtttgatttt
    28021 acgtactaag gtagtaaact gattttggat taaacaataa agcgtgcagt ctcaacagac
    28081 tgcacgcttt attgtttctc actggatcca ttttatttca tcaagtggat aatagacaaa
    28141 tcttgcttta cctagaatat cagttttatt gatcgtgcca aacgaacgac tatcttttga
    28201 aactcttcga ttatctccaa gtacaaaata attatcttcc ggaagcttgg attgtcccgt
    28261 tagctcttgt agtgtaaagt ccgtcgtata ggatgcattt gcatgatctg atttcaggtt
    28321 tttagttaga taaggttcct taactacttt cccattgatc ttcaattgat cattttggta
    28381 gctgacattt tctcctggaa gaccgattac tcgtttgata taaatcgtgc catctgctaa
    28441 ttgaaaaaca acaatatcaa atcgtctgat ctccgaaaat ttttccatca caaccatatc
    28501 accttgcttc aacacgtttt ccattgaatt gccttccact ggtaccggga tgaataaaaa
    28561 tccccgcaac atgaacgcaa ggaatacaga aacaagtaag tatttgaata caagccagaa
    28621 gcgattaata tagcgttgtt tttttgtcaa tctctatcaa tccatccctc tttttttcat
    28681 tctttcttta ttataagaaa aatatcaaaa aaatgcgact agatagctga aataaaaata
    28741 attctttcaa ttaggctatt ggacaaaaaa cttttttgat cataaagtac aaaactatct
    28801 cttatatgtc atgattattt tctaaatatt tttcccatgc ttcgtcaaag atatccatgg
    28861 acggcaaata gtcgacattc agttccagat aattagaaat ttcttcgtag ctagagctgt
    28921 gcttaggaaa ttggatgtct ttaccggctt ctgtagcgaa aacagataaa gcatcatgtg
    28981 tgccaccgcc tcgcagtgtc attaaataat gaaaaaagct tcgattcacc cttcgttcct
    29041 ccaattttct tcgataaagg cagcccggcg tgcatggttt cttttaaaat tttcaggatg
    29101 ctttttataa tactcttgat ggtcttcttc tgctagataa aatggttggg ctggttcgat
    29161 ttttgtaacg attggctgtt cgaaacgtcc actttcagct aactgtttct tacttttttc
    29221 agcaatgatc cgctgttctt ctgtcaaata aaagatcacc gggcgataat tgtcaccgcg
    29281 atcctcgaat tgtccgaaag cgtcagttgg atcagtttgc tgccagtaaa gttctactaa
    29341 ttgttcatat gaaatcaatg ttggatcata gataatctcc actgcttctg tatgtccagt
    29401 cgtttggctt tttacttgtt cataagtcgg atctggtaca tgccctccag tatatcctga
    29461 catcactgta tggataccag gaagcgtgtc gaaaggctgg atcatacacc agaagcagcc
    29521 tcctgcaaat attgctcgtt ccattatgat tccccctcta atactactca tccgatgatt
    29581 ttttattttc ttttgtcttg ctttcagtac tgctggtgct agttttgttg gtatttgagt
    29641 tgtttgaagc atcagaggta tcttttggca aatatagact aaaccgaatg tcatcattta
    29701 ctaagtcgat tttatctgct ttaatataca tcccgttttg cattcggaat tgatccaacc
    29761 taagcatgat agtcagatca tctgtattga cttcgatcca atctggtaat ttatagtttc
    29821 tcttgatcat attcatcaca tctttgattg gcaaactcaa tgttccgata gaaagacttc
    29881 tagcttttag ttggacattt ccgttttcca tcacatacgg atcaaaatat aaatagaaat
    29941 cgataggaaa acctagtact tcgaaagtac cattgagta
//
LOCUS       DAZKNO010000011        29757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000011 DAZKNO010000000
VERSION     DAZKNO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.011"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            211..1080
                     /locus_tag="ACG8MY_000361"
     CDS             211..1080
                     /locus_tag="ACG8MY_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0429593.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1289..2314
                     /locus_tag="ACG8MY_000362"
     CDS             1289..2314
                     /locus_tag="ACG8MY_000362"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0429594.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2353..3303
                     /gene="manA"
                     /locus_tag="ACG8MY_000363"
     CDS             2353..3303
                     /gene="manA"
                     /locus_tag="ACG8MY_000363"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0429595.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3397..5100
                     /locus_tag="ACG8MY_000364"
     CDS             3397..5100
                     /locus_tag="ACG8MY_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0429596.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5102..7135
                     /locus_tag="ACG8MY_000365"
     CDS             5102..7135
                     /locus_tag="ACG8MY_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0429597.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7146..7586
                     /locus_tag="ACG8MY_000366"
     CDS             7146..7586
                     /locus_tag="ACG8MY_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0429598.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7786..8913)
                     /locus_tag="ACG8MY_000367"
     CDS             complement(7786..8913)
                     /locus_tag="ACG8MY_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429599.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8916..9593)
                     /locus_tag="ACG8MY_000368"
     CDS             complement(8916..9593)
                     /locus_tag="ACG8MY_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0429600.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9857..11623
                     /locus_tag="ACG8MY_000369"
     CDS             9857..11623
                     /locus_tag="ACG8MY_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0429601.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11638..12075
                     /locus_tag="ACG8MY_000370"
     CDS             11638..12075
                     /locus_tag="ACG8MY_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0429602.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12088..13239
                     /locus_tag="ACG8MY_000371"
     CDS             12088..13239
                     /locus_tag="ACG8MY_000371"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0429603.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13301..13999)
                     /locus_tag="ACG8MY_000372"
     CDS             complement(13301..13999)
                     /locus_tag="ACG8MY_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0429604.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13992..15344)
                     /locus_tag="ACG8MY_000373"
     CDS             complement(13992..15344)
                     /locus_tag="ACG8MY_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0429605.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15567..16967)
                     /locus_tag="ACG8MY_000374"
     CDS             complement(15567..16967)
                     /locus_tag="ACG8MY_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0429606.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17128..17865
                     /locus_tag="ACG8MY_000375"
     CDS             17128..17865
                     /locus_tag="ACG8MY_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0429607.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17990..18469
                     /locus_tag="ACG8MY_000376"
     CDS             17990..18469
                     /locus_tag="ACG8MY_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0429608.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18466..19233
                     /locus_tag="ACG8MY_000377"
     CDS             18466..19233
                     /locus_tag="ACG8MY_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0429609.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19463..20032
                     /gene="lepB"
                     /locus_tag="ACG8MY_000378"
     CDS             19463..20032
                     /gene="lepB"
                     /locus_tag="ACG8MY_000378"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0429610.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20136..20738)
                     /locus_tag="ACG8MY_000379"
     CDS             complement(20136..20738)
                     /locus_tag="ACG8MY_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0429611.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20844..21074
                     /locus_tag="ACG8MY_000380"
     CDS             20844..21074
                     /locus_tag="ACG8MY_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429612.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            21065..21313
                     /locus_tag="ACG8MY_000381"
     CDS             21065..21313
                     /locus_tag="ACG8MY_000381"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0429613.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            21419..22003
                     /locus_tag="ACG8MY_000382"
     CDS             21419..22003
                     /locus_tag="ACG8MY_000382"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0429614.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22006..23079
                     /gene="prfA"
                     /locus_tag="ACG8MY_000383"
     CDS             22006..23079
                     /gene="prfA"
                     /locus_tag="ACG8MY_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0429615.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23072..23911
                     /gene="prmC"
                     /locus_tag="ACG8MY_000384"
     CDS             23072..23911
                     /gene="prmC"
                     /locus_tag="ACG8MY_000384"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0429616.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23904..24083
                     /locus_tag="ACG8MY_000385"
     CDS             23904..24083
                     /locus_tag="ACG8MY_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429617.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24105..25130
                     /locus_tag="ACG8MY_000386"
     CDS             24105..25130
                     /locus_tag="ACG8MY_000386"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0429618.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25305..26549
                     /gene="glyA"
                     /locus_tag="ACG8MY_000387"
     CDS             25305..26549
                     /gene="glyA"
                     /locus_tag="ACG8MY_000387"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0429619.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26702..27649
                     /locus_tag="ACG8MY_000388"
     CDS             26702..27649
                     /locus_tag="ACG8MY_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0429620.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27783..28412
                     /gene="upp"
                     /locus_tag="ACG8MY_000389"
     CDS             27783..28412
                     /gene="upp"
                     /locus_tag="ACG8MY_000389"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0429621.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28551..28793)
                     /locus_tag="ACG8MY_000390"
     CDS             complement(28551..28793)
                     /locus_tag="ACG8MY_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429622.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28914..28986
                     /locus_tag="ACG8MY_000391"
     tRNA            28914..28986
                     /locus_tag="ACG8MY_000391"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28947..28949,aa:Thr,seq:cgt)
     gene            complement(29105..29743)
                     /locus_tag="ACG8MY_000392"
     CDS             complement(29105..29743)
                     /locus_tag="ACG8MY_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0429623.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT        10117 a         5253 c         6058 g         8329 t
ORIGIN      
        1 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
       61 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      121 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      181 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      241 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      301 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      361 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      421 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      481 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      541 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      601 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      661 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      721 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      781 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      841 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      901 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
      961 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1021 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1081 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1141 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1201 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1261 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1321 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1381 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1441 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1501 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1561 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1621 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1681 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1741 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1801 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1861 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1921 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     1981 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2041 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2101 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2161 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2221 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2281 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2341 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2401 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2461 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2521 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2581 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2641 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2701 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2761 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2821 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2881 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     2941 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3001 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3061 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3121 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3181 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3241 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3301 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3361 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3421 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3481 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3541 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3601 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3661 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3721 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3781 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3841 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3901 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     3961 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4021 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4081 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4141 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4201 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4261 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4321 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4381 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4441 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4501 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4561 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4621 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4681 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4741 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4801 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4861 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4921 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     4981 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5041 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5101 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5161 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5221 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5281 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5341 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5401 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5461 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5521 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5581 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5641 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5701 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5761 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5821 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5881 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     5941 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6001 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6061 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6121 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6181 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6241 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6301 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6361 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6421 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6481 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6541 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6601 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6661 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6721 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6781 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6841 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6901 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     6961 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7021 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7081 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7141 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7201 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7261 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7321 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7381 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7441 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7501 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7561 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7621 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7681 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7741 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7801 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7861 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7921 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     7981 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8041 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8101 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8161 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8221 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8281 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8341 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8401 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8461 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8521 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8581 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8641 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8701 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8761 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8821 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8881 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     8941 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9001 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9061 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9121 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9181 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9241 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9301 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9361 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9421 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9481 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9541 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9601 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9661 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9721 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
     9781 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9841 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
     9901 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
     9961 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    10021 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    10081 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    10141 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    10201 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    10261 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    10321 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    10381 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    10441 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    10501 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    10561 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    10621 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    10681 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    10741 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    10801 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    10861 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    10921 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    10981 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    11041 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    11101 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    11161 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    11221 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    11281 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    11341 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    11401 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    11461 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    11521 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    11581 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    11641 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    11701 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    11761 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    11821 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    11881 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    11941 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    12001 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    12061 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    12121 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    12181 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    12241 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    12301 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    12361 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    12421 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    12481 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    12541 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    12601 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    12661 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    12721 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    12781 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    12841 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    12901 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    12961 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    13021 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    13081 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    13141 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    13201 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    13261 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    13321 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    13381 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    13441 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    13501 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    13561 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    13621 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    13681 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    13741 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    13801 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    13861 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    13921 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    13981 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    14041 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    14101 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    14161 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    14221 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    14281 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    14341 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    14401 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    14461 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    14521 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    14581 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    14641 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    14701 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    14761 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    14821 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    14881 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    14941 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    15001 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    15061 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    15121 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    15181 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    15241 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    15301 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    15361 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    15421 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    15481 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    15541 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    15601 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    15661 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    15721 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    15781 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    15841 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    15901 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    15961 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    16021 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    16081 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    16141 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    16201 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    16261 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    16321 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    16381 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    16441 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    16501 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    16561 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    16621 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    16681 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    16741 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    16801 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    16861 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    16921 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    16981 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    17041 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    17101 gaaaaaagaa ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg
    17161 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    17221 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca
    17281 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    17341 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    17401 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    17461 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    17521 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    17581 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    17641 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    17701 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    17761 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    17821 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    17881 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    17941 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    18001 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    18061 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    18121 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    18181 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    18241 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    18301 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    18361 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    18421 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    18481 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    18541 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    18601 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    18661 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    18721 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    18781 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    18841 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    18901 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    18961 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    19021 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    19081 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    19141 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    19201 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    19261 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    19321 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    19381 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    19441 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    19501 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    19561 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    19621 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    19681 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    19741 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    19801 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    19861 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    19921 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    19981 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    20041 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    20101 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    20161 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    20221 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    20281 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    20341 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    20401 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    20461 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    20521 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    20581 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    20641 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    20701 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    20761 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    20821 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    20881 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    20941 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    21001 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    21061 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    21121 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    21181 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    21241 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    21301 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    21361 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    21421 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    21481 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    21541 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    21601 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    21661 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    21721 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    21781 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    21841 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    21901 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    21961 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    22021 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    22081 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    22141 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    22201 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    22261 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    22321 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    22381 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    22441 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    22501 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    22561 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    22621 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    22681 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    22741 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    22801 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    22861 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    22921 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    22981 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    23041 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    23101 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    23161 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    23221 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    23281 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    23341 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    23401 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    23461 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    23521 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    23581 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    23641 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    23701 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    23761 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    23821 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    23881 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    23941 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    24001 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    24061 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    24121 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    24181 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    24241 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    24301 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    24361 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    24421 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    24481 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    24541 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    24601 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    24661 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    24721 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    24781 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    24841 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    24901 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    24961 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    25021 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    25081 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    25141 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    25201 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    25261 tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa
    25321 cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt
    25381 tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta
    25441 tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat
    25501 ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat
    25561 ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg
    25621 tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg
    25681 gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga
    25741 caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat
    25801 tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa
    25861 tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag
    25921 cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata
    25981 aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa
    26041 aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg
    26101 gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa
    26161 tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca
    26221 tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg
    26281 gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc
    26341 cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca
    26401 ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat
    26461 tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa
    26521 ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt
    26581 tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga
    26641 tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt
    26701 catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc
    26761 tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca
    26821 agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga
    26881 atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt
    26941 tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt
    27001 gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc
    27061 accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa
    27121 taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt
    27181 aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat
    27241 atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca
    27301 tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata
    27361 tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa
    27421 tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga
    27481 tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg
    27541 cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac
    27601 gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt
    27661 ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag
    27721 tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa
    27781 aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta actattattc
    27841 gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga
    27901 tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca
    27961 ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg
    28021 caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata
    28081 tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag
    28141 aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag
    28201 caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt
    28261 tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct
    28321 atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg
    28381 atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac
    28441 ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc
    28501 tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt
    28561 tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt
    28621 tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca
    28681 gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca
    28741 atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct
    28801 gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc
    28861 catcggactc tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct
    28921 tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag
    28981 ctggcattaa aagagaagac agatatatca aggattttta atccattgat acatctgtct
    29041 ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa
    29101 aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg
    29161 cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca
    29221 ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac
    29281 tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact
    29341 tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag
    29401 gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc
    29461 atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa
    29521 aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt
    29581 ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa
    29641 taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata
    29701 tccctttttt aagttctatt ttttttccta aaatgttatt catgataata actccta
//
LOCUS       DAZKNO010000012        29538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000012 DAZKNO010000000
VERSION     DAZKNO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.012"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..985
                     /locus_tag="ACG8MY_000393"
     CDS             116..985
                     /locus_tag="ACG8MY_000393"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0429624.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1150..2421
                     /locus_tag="ACG8MY_000394"
     CDS             1150..2421
                     /locus_tag="ACG8MY_000394"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0429625.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2418..3713
                     /gene="rho"
                     /locus_tag="ACG8MY_000395"
     CDS             2418..3713
                     /gene="rho"
                     /locus_tag="ACG8MY_000395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0429626.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3812..4075
                     /locus_tag="ACG8MY_000396"
     CDS             3812..4075
                     /locus_tag="ACG8MY_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0429627.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4520..4969
                     /locus_tag="ACG8MY_000397"
     CDS             4520..4969
                     /locus_tag="ACG8MY_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0429628.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            5026..6444
                     /locus_tag="ACG8MY_000398"
     CDS             5026..6444
                     /locus_tag="ACG8MY_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0429629.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6444..7304
                     /locus_tag="ACG8MY_000399"
     CDS             6444..7304
                     /locus_tag="ACG8MY_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0429630.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7478..8251
                     /locus_tag="ACG8MY_000400"
     CDS             7478..8251
                     /locus_tag="ACG8MY_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0429631.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8398..8763
                     /locus_tag="ACG8MY_000401"
     CDS             8398..8763
                     /locus_tag="ACG8MY_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0429632.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8911..10119)
                     /locus_tag="ACG8MY_000402"
     CDS             complement(8911..10119)
                     /locus_tag="ACG8MY_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0429633.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10138..10416)
                     /locus_tag="ACG8MY_000403"
     CDS             complement(10138..10416)
                     /locus_tag="ACG8MY_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429634.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10609..11265
                     /locus_tag="ACG8MY_000404"
     CDS             10609..11265
                     /locus_tag="ACG8MY_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0429635.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11707..12504)
                     /locus_tag="ACG8MY_000405"
     CDS             complement(11707..12504)
                     /locus_tag="ACG8MY_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0429636.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12501..13685)
                     /locus_tag="ACG8MY_000406"
     CDS             complement(12501..13685)
                     /locus_tag="ACG8MY_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0429637.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13710..14957)
                     /locus_tag="ACG8MY_000407"
     CDS             complement(13710..14957)
                     /locus_tag="ACG8MY_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0429638.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15172..16917
                     /gene="dnaX"
                     /locus_tag="ACG8MY_000408"
     CDS             15172..16917
                     /gene="dnaX"
                     /locus_tag="ACG8MY_000408"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0429639.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16937..17251
                     /locus_tag="ACG8MY_000409"
     CDS             16937..17251
                     /locus_tag="ACG8MY_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0429640.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17276..17872
                     /gene="recR"
                     /locus_tag="ACG8MY_000410"
     CDS             17276..17872
                     /gene="recR"
                     /locus_tag="ACG8MY_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0429641.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17936..18580
                     /gene="tmk"
                     /locus_tag="ACG8MY_000411"
     CDS             17936..18580
                     /gene="tmk"
                     /locus_tag="ACG8MY_000411"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0429642.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18595..18924
                     /locus_tag="ACG8MY_000412"
     CDS             18595..18924
                     /locus_tag="ACG8MY_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0429643.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18938..19876
                     /gene="holB"
                     /locus_tag="ACG8MY_000413"
     CDS             18938..19876
                     /gene="holB"
                     /locus_tag="ACG8MY_000413"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0429644.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19912..20736
                     /locus_tag="ACG8MY_000414"
     CDS             19912..20736
                     /locus_tag="ACG8MY_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0429645.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20729..21076
                     /locus_tag="ACG8MY_000415"
     CDS             20729..21076
                     /locus_tag="ACG8MY_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0429646.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21145..22017
                     /gene="rsmI"
                     /locus_tag="ACG8MY_000416"
     CDS             21145..22017
                     /gene="rsmI"
                     /locus_tag="ACG8MY_000416"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0429647.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22126..23247
                     /gene="dinB"
                     /locus_tag="ACG8MY_000417"
     CDS             22126..23247
                     /gene="dinB"
                     /locus_tag="ACG8MY_000417"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0429648.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23301..23903)
                     /gene="nrdG"
                     /locus_tag="ACG8MY_000418"
     CDS             complement(23301..23903)
                     /gene="nrdG"
                     /locus_tag="ACG8MY_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0429649.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24218..26407)
                     /gene="nrdD"
                     /locus_tag="ACG8MY_000419"
     CDS             complement(24218..26407)
                     /gene="nrdD"
                     /locus_tag="ACG8MY_000419"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0429650.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIAEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26733..27500
                     /locus_tag="ACG8MY_000420"
     CDS             26733..27500
                     /locus_tag="ACG8MY_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429651.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27490..29532
                     /locus_tag="ACG8MY_000421"
     CDS             27490..29532
                     /locus_tag="ACG8MY_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429652.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         9460 a         5313 c         6185 g         8580 t
ORIGIN      
        1 ttttcatttt cattgatttt agtagaaaac taccgaaatg tttggtaaaa tagggtcgtt
       61 ggttaagaat tgtcttaaca aatttagttt tacaaaactt aggaggaatt ttattatgcc
      121 agtagtatca ggagctgaat ttttaaaagc tgctcgtaaa ggcggttacg ctgtcggcgg
      181 attcaacaca aacaacttgg aatggactca agcaatcctt gaagcggctg aagcaaaaca
      241 agcaccagta cttatccaaa catcaatggg tgcagctaaa tacatgggtg gttacaaaat
      301 ctgtaaagat ttaatcactg atttagttga agcaatgaac atcactgttc cagttgctat
      361 ccacttagac catggtgatt acgaagctgc tttggaatgt atcgaattag gctatacttc
      421 agtaatgttc gatggttcac atctaccatt tgaagaaaac ttgaaattag ctaaagacgt
      481 tgttgaaaaa gcacatgcaa aaggtatctc agttgaatgt gaagttggtt caatcggcgg
      541 agaagaagac ggaatcatcg gtaacggcga attagctgat attgaagaat gcaaacaaat
      601 ggttgctaca ggtatcgact acttagcttg cggtatcggt aacatccatg gtcaatatcc
      661 agaaaactgg aaaggattag cttttgacca cttacaagca attgctgatg ctgtaggtcc
      721 agacatgcct ttagtattac atggcggatc aggtattcct caagatcaaa tcgaaaaagc
      781 tatctcaatg ggtatctcaa aagtaaatgt taacactgaa ttccaattat catttgctgc
      841 agctactcgt aaatatatcg aagctggtaa agataaagaa ggtaaaggat tcgaccctcg
      901 taaattatta gctccaggta aagcagcaat cgttgaagat gctaaatctc atatcgactg
      961 gttcggttca gctaacaaag cgtaagcatc cacataacag atacgatata taggaatgga
     1021 gaacgacttt tgaagtcgct ctccattctt ttttacttaa aacggcagac catgctcgtt
     1081 tctttttttt atctatgata aaatacaagc atatccatga aaaaagatta ataaacaagg
     1141 tggaaacaga tgaaaaaaat cgtaatcaaa ggaaaccgac ctttagctgg tgaagtaacg
     1201 atcagcggtg caaaaaatag tgcagtcgca ttgattccag ctgcaatctt agctgattcc
     1261 ccagtgatct tggaaggtgt gccggacata caagatgttc attcattaat tgaaatttta
     1321 gagatcatgg gcgcaactgt ccatttttcc aacaacatac tagaaatcga tcctcgagaa
     1381 gtcgtatcga ttccgatgcc gcacggaaaa atcaatagtt tgcgtgcttc ttattatttt
     1441 atgggaacac tgcttggtaa atttggcgaa gcggtagtag gcctgccagg tggctgttat
     1501 ttaggcccta gaccgattga cctacacgtc aaaggatttg aagctctagg tgctgaagtg
     1561 acaaatgaac atggtgccat gtatttacgg acagaaaata aaggattgcg tggcaatcgg
     1621 attttcatgg acgtggtttc agtcggagca acgatcaatg tgatgttagc tgctgtaaaa
     1681 gcaaaaggac agacagtaat tgaaaacgct gcacgagaac cggaaatcat agatgtggca
     1741 actttactga ataatatggg ggcaaaagtt cgaggagcgg gtacagatgt gatccgtatc
     1801 gaaggagtcg aaacattaca tggctgtcgg catttcatga ttcctgaccg catcgaagca
     1861 ggaacctatc tagcattagc agctgcagta ggaaatggta tcaaagtgaa aaatgtgatc
     1921 tttgaacatt tggaaagctt tatagcaaaa ttgcaggaaa ttggtgtcca tatgaaaatc
     1981 agcgaagatg aaatcgaagt ttacccctcg aaagaattaa aacccgcaaa catcatgacc
     2041 tatccttatc caggatttgc cacagatctg cagcagccac ttactgcgtt actattgatg
     2101 actacaggca cttcagaaat catcgatacg atctatgcga aacgtgtgaa ccatgtgccg
     2161 gaactagcac gaatgggcgc tgatattacc gtggaaggaa atatgatcat cgtgaatggt
     2221 ccaaataaac ttcacggcac agaagtcgtg gcttctgatc ttcgggcagg tgcttgttta
     2281 gtgattgctg gactattggc agaagggaca acgacgattt acaatgtaga ttatatctta
     2341 cgcggttatg atcatattat cgagaaacta acagccttag gggcatcaat tgaaatgata
     2401 gaggaagtgg gagctgaatg agcgattatt tgacaatggc agaactagag aacaaaacgt
     2461 taaaagaagt ttatagttat gccaaagaat ttaaaattcc ttattacagt aagatgaata
     2521 aaaaggaatt atctctagct gtgattcgtg cacaagcaga gaaacaagga ttttattata
     2581 tggaaggaat attggatatc gttggtcaag atggttacgg ttttttacgg ccaatcaatt
     2641 atggaccaag tgcagaagat atctatattt ctgcctcaca aatcagacga ttcagtttga
     2701 gaaatgggga caaagtggca ggaaaagccc gtccacctaa agaatcggaa cgttattatg
     2761 gtttgatgca cgtggaaagc gttaatggaa aagatccgga agaagcaaaa gaacgtccgc
     2821 attttccagc cttaacacct ctttatccgc aaagacagct tcaactggaa acaacaccgc
     2881 aaagactatc tacccgaatg atcgatatct tttctccaat cggttttggt cagcgtggat
     2941 tgatcgtcgc accgccaaaa gcagggaaaa cttctgtatt aaaagaaatc gcaaatggta
     3001 tcaccgaaaa ctatccggaa gtagaattaa tcgttctact gattgatgaa cgaccggaag
     3061 aagtaacgga tctagaacga agcgtgaaag gcgacgtggt atcttctaca tttgatctgc
     3121 agccgcaaaa ccatactcga gtttctgaat tagtattgga acgcgcaatg cgtttagtcg
     3181 aagataaacg agatgtcgtt atcttgatgg atagtattac tagacttgct cgggcctata
     3241 acttagtcgt tccaccaagc ggacgtacat tgagtggcgg gattgaccca gccgcattgt
     3301 ataaacctaa gaaatttttc ggggcagcaa gaaacatcga agaaggtggc agtttgacga
     3361 ttttagcgac cgctttagtc gatacaggaa gccgtatgga tgacgtgatc tatgaagaat
     3421 tcaaaggaac tggaaatcta gaattacaac tttcacgtga attatctgaa cgtcggatct
     3481 tcccagccat cgatatcaaa aaatcaggta cccgaaaaga agaattattg atgccaagtg
     3541 aagaattaga agaaatttgg aaacttcgaa aaaatatgac tggagattca ttagaatata
     3601 cggaacaact gatccgcttt ttgcgtaaaa caaaaaacaa tcagcaattt ttcaaagaat
     3661 ttcaagatgt ctcctttggt aaaaaaaatc cgaccagaaa tctgaaaaga taattgcaac
     3721 cttgactgaa acatgttaag atgttaatgt tgtaaatcga caaatcaact ctggttcagc
     3781 aaatggagca gggcaaagga gcgaaaaaat catgaaagaa aacatccatc cagattacca
     3841 cccagtagta ttcatggatt caacaactgg tttcaaattc ttgtcaggtt caacaaaaac
     3901 ttcacaagaa acagttgaat gggaagacgg taatacatac ccagtcatcc gtgtcgaagt
     3961 aacttctgac tcacatccat tctacactgg acgtcaaaaa ttcacacaag cagacggacg
     4021 tgtggaccgt ttcaacaaaa aatacggtct caaagacgca aacgctgcgg aataataaaa
     4081 acgctgataa ctcaacgttt agcagactat ctgataggga tagtctgttt ttttatgttt
     4141 tttgccaatt taaaacttcg tttgatgctg aaaaagtcgt aggatcataa tgcacaaaac
     4201 aatgaaaaac tagtatatag ttttaattat ccacttgata aaaacacctt atattaacga
     4261 aatgaagagg ggttatttcg aagtaaaaaa ccatttacct aaaattagaa gctaagcttt
     4321 cctttcaagg aaggcttttt gttttgttat agacttgcga ttgtttgtga gctagatcgt
     4381 tcattttaat gacttataca cgcggatgat gggtcttttt cctctgttca ttcataatat
     4441 tgaacggtac agctagcgat gctatactga cctcagttcc aagaaaacgt taacattttt
     4501 tgtgggggag aggtttttta tgaggtttga tcaattgatc gcagaagaac tgattgtttt
     4561 cgatccatcg atagattcaa aaggatcatt atttgagcga ataggggatg tgttgaaaaa
     4621 aacaaataag gtaagaaatg ttcaaaaagt cgttaaggca ctattcaaac gagaggagga
     4681 gatctccaca ggaatagaag atggatttgg gattccccat gcaaaaagca aatatgtgac
     4741 aaaaccagca ctgtgttttt ttcatacagc caaattaacg gactatagcg ggatagatga
     4801 aatgccaatt gaatatattt ttgccatcgt agttcctgaa aaatcagcag atatgcatct
     4861 agagattctc agcacactct ccagacgatt gatgaatgca gattttagaa agggaataaa
     4921 agaagccact acgccagcag atatcatcag acagattagt caagaatgaa aatagtagag
     4981 tctttgtccg agtatcgata gtgaagaaaa aggaggaaaa taatcatgaa aatcgttggt
     5041 gtaactgctt gcccgactgg aattgctcat acttatatgt cggcagaaaa attagaagta
     5101 acagcaagaa atctaaacca tcaagcaaaa ttcgaaacac aaggtgtaaa aacagaaaat
     5161 cagttatctg aacaagaaat caaagaggca gatgcaatca ttcttgcagt agataaagaa
     5221 atcgaattgg accgatttgc cgggaaaaaa gtaaagcgtg tctctacaag ccgagcaatc
     5281 aaagaaccgc aagtagtaat agacgaagca ttgagagata ttggcgtatt cgttgtgtct
     5341 aatgaaaaag aaccagctgc aaatgagaaa aagccgacaa tctataatca tttcatgaat
     5401 ggtgtgaatt acatgcttcc atttgtgatc gcaggcggaa tcatcatcgc actgagtttt
     5461 gcctttggga ttacagcggc tgatcccaac tcagcagact ataatgtgtt agcagctgca
     5521 ttttcaagga tcggaggaga tacagcgttt gccatgatgg ttcctgcgtt aggcgcgggg
     5581 attgctacat cgattgctgg aaaagcaggt tttgccccag gaattgttgc cggattactg
     5641 gcctctacag gtggttcagg ttttcttggc ggaatgattg gtggcgtgct agcagggtat
     5701 atctgtgatt ttctggcaaa taaaattagc gtaaaaaaag aattttctgc tatttatcag
     5761 ctgattgtcg tccccttttt atcgatctta attattgggc ttttgatggt atttgtgatt
     5821 gaacaaccga tcacatgggt gttggatgct ttgacaaatt ggctgaatag cttgggaaat
     5881 acatccgggc tattgttcgg attacttatc gggatcatga tggcagctga tatgggtggg
     5941 ccgatcaata aatcgatttc gactttctcg atcggtttga tgtcagctgg agtaaatgca
     6001 ccgattgctg cttgtatggc tgcaggaatg acacctcccc taggtattgc tttagccact
     6061 ttattattca aacataaatt taccaaagaa gaacggactt caggtcaatc atgctggatt
     6121 ttaggcgctt cgtatattac agaaggtgct ataccgtttg cggtatctga cccgatccgc
     6181 gtgataccta gtttgatgac aggttcagca gtcgctgcag ggatttccat ggcagtagga
     6241 gttacttcaa tggcacctca tggagggatt tgggtgatgc taattccaaa tgttatgaac
     6301 aatttaccta tgtatgtctt ggcaattgca tcaggtacag tcactacagc ctttatgctt
     6361 ggactattga aaaaaacagt gaaagagaaa aaacaagaca agtggacgaa aaatgaaaat
     6421 ctaacaaatg aggtggaagc gtaatgttat atacaatgaa agagttattg aaagtggcta
     6481 aagagaatca atttgcagta ccagccttca atatttgcag ctatgatatg atgaaagcaa
     6541 tcatcgaaga agcagagaga ttagaagcac cagtaatctt agaaattcat cctgatgaaa
     6601 tcgcctattt aagtgatgag tttgttgcgg cagtaagagc ctatgcttat acaagtaaac
     6661 tgccgatcgt tatccacttg gatcatggcg gtacgataga agatgtctta cgggcgattc
     6721 gaaacggcta ttcttccgtg atgatcgatg cgtctttgaa atcattcgac gacaatgtac
     6781 agattacgaa aaaagtggtt gcgttagctc atgcggtaaa tgtctcagtg gaggctgagc
     6841 ttggaacgat tgggaataat ggttctgctg aaggaggtgc tgaaacaatc atttatacag
     6901 atcctgatca agcgaagatt tttgtgaaag aaacaggaat cgatacactt gctgtagcaa
     6961 ttggaacagc gcatggcttg tatccgaaaa acagaactcc aaaattaaat atcgacttac
     7021 tgaaagaatt gaaccaacaa atcgatcttc catttgtttt acatggcggt tcaggtaatc
     7081 cggacgcaga agtcagcgaa gcagtaaaat acggagttgc caaagtgaat ttgagctcag
     7141 atcttaaaag cgtctttttc aatgctttga gagaaatatt gagtgaaaat ccatcgatgt
     7201 acgagccgaa tgcaatttat ccagcagcta ataaaaaggt acaagaagta gtcaaacata
     7261 aaatggaaat tttgaacaca gtaggcaagg ctagtttata ctaaaaataa aaatgtgtgg
     7321 tcaagagggt atgacaatag tcttgtcata ccctcttaac cacacacgat gttcagaagt
     7381 agttttttcg aaaaatttct tcttatttct cgaagccaat cacttctgtc acaacctctt
     7441 ttttcctgtt actatataaa gtaaatgggg tgattttatg ttgaatgaag tggagaatag
     7501 acagcaggag attgtgaatt atctaaaaaa caaccaattt gcaagagtca ccgatttgat
     7561 cgagttagta aattatagcg aagccacgat taaacgtgac cttgtttttc tggaaaaaag
     7621 tggattgatc cgtagagttc gcggtggcgc catgcttatt gacaatcgaa aaattgacgt
     7681 gccttacctg atgaagatca ccaaattaga agaagaaaca gaaaaacgta agattgcaga
     7741 tacagcttct tctttgattc gagatgatat ggtcttattc ttggattcca gtacaacctc
     7801 tttacactta gtcagaaacc tagcaagatt tgatggtttg cagatcatta cgaatggcat
     7861 cgtcacagct gctatgttga gtgaatatac aacggcaaaa gtatctgtat tgggaggggc
     7921 aattgttccg aaacgagcaa caatcaacgg ttcaaaagcc tataacgatg cattgtcata
     7981 ttatgcggat ttagcgataa tgagttgtcg cgggctagat ttcagccaag gagtaacaga
     8041 aactcatgaa ggagaggcaa tggtcaaaaa agcttttaga aaacaatcag accggcttgc
     8101 cgttttagtt acacagcaaa aaattgcaca taaattcatg catcaagcca ttgcttttca
     8161 tgaaatcgat tatctggtga caagtaaaga actgacaaaa gaacagtatg cgaaagtaaa
     8221 ggaacaccat atcgaatgtc tgtacggata aattattacg tgaatgttct aagtggcttc
     8281 ctcattttta gataggacct tatctttgct cgcttctctc agccgatggt ataataatga
     8341 aaaggttatc ttgttagata atcttacttt tttatccagg agaaaggagt gcagaaaatg
     8401 aaacatgtga aggcgttggt agtgaaggca atcatgatct gggcgattct gtgggtcgtg
     8461 ctgacaggtt tatacggtgt tagctttatg gattcaacga ttgttggggt aattattgta
     8521 gtcatgatct atgtgctagg ggatctgttg atcttacgta aagtagggaa catcgcagcg
     8581 acgatcgcag atgctggttc agctgcagtt atcttgtggc tctatctata ttttatgaat
     8641 gatacagtcg atatttggat gaaagtattg attcctgctc tattgatcgg tgtggcagaa
     8701 tggttcttcc acaagtggtt gctaagtcaa ggcatcgtac cagatgaacg gaaaatggaa
     8761 taaatcaaca gttaaactat tcttatcgat aaatgaaagc tttaaaaata aatagatgtt
     8821 taattaagaa taatcataaa ctatttttac tcaaaaaacc gctcagatta ttttcagatc
     8881 tgaacggttt tttttatcta ttcatcaaga tcaaatttca tttttcaatg attcaatcaa
     8941 ttcgccctct gcttcatgac tatgttttcg gccattaaca agatagagtg ctgtacatat
     9001 caatggaatg actccggcaa tcacatacaa tcctctaaat gaagtaagag attttagcat
     9061 ccccatcaaa taaggaccaa cgcctaagcc taggtctaaa ccaataaagt aggtagataa
     9121 agcaatactg attcgatgtt cgcggacgat ttttaaacaa acagcctgtc catttgacat
     9181 aaacgtacca taacctaggc cgactaatgc tccagagata agcaatgtca cactattcgt
     9241 cgttacactc agtaaaaata atcctgctgt taagaataaa tagctaggat acatcacgta
     9301 ttgttctcct ttgacatcga aaatccttcc agtcaatgga cgtgtaaatg tgatgaccaa
     9361 agcatatacg acgaagaaaa atgacccagc agcaactaag tggattactt ttgtataaga
     9421 agataaaaat gataaaacac ttgaataaga aagacccatc aaaaaggcaa ttcctgtaat
     9481 gaaaagtgct ttcttttcaa taaaactatt gatattccat gagcgaagag atattttttg
     9541 ttcttcagat aatggaatat tttttacagg aaagacaagg caggcaatcg taacaaagaa
     9601 gaccagtaca attgaaaacc agataatgaa acgaaaatca gtcatattca gcaaaatcat
     9661 tcctaaaaat ggcccaacag cggcagctaa actagtactt aatccataat aattgatgcc
     9721 ttcccctttt ttagatgcag ggatatatgc agtaacgata gcatttgtag ctgtagaaac
     9781 tgtaccgtag ccgaatccat tcaagaatcg aactaaatac aatgccccga tagttggcgt
     9841 ataaagatat gctaatgttg aaaggagata gaagatcgcg ccgcctctta aggtaaactt
     9901 ccgaccaaat agttctaact gtttccccat gaataagcgt gccagcaatg ttccgatgat
     9961 atagatccca gaagcaaaac ctgcttggct caagctggcg tgtaaggtgt cttgcgcaat
    10021 gactgccatg atgaccatca acagataata aatcaggtag acaataaaat tgatcaatgt
    10081 gattccaata aaatatttat taaataattt cttttccatg tatgttatac attcccctta
    10141 agctgaatat gcttttaatt tttgtatttg aacttctaca tcgtgataat tggatggata
    10201 gcgatcaaca agttggcgaa taaataaatc tcttgtagac ataaacagat ttaaaaattc
    10261 ttcgcattcg tttgttggat tctcatccac tgctttgatc aattcgatcg agttttgata
    10321 cgcatcattt aaaaagacta aagaagcttt catatcaaat cttgtttgat atgctgtttc
    10381 taatcgatta atgattgtgt tcatccagta attcatgttt tttccctcta ttcttaaaaa
    10441 attttacttc tgcagtataa cgaataaaaa aaaggaaaaa aatcgcataa gtgatggaaa
    10501 aaggtactta acaaatgtta atatcattag taaaatacct ttggacttta aaaagtttta
    10561 aagtactgta tgatcttttt agatgaaagt ttggatgggg gagcatcatt ggatagtaga
    10621 gtcttaaagg aatatttgga taaaaatgat tttccgatta tacgcaaagg atatcataaa
    10681 tatttgacat tcgaagggat ggaagattcc catacgtata tcttgaaaga gggaacggta
    10741 aaagcaagtg ttatttcaaa tgatgggaga gaatttaacc ttcgctatat caaagatctt
    10801 gaaatcgtct ctcttttaaa agacgaatat tcacaattca tcgattcgcc gtataatatt
    10861 cgagttgaat cagaatatgc agagttttat cgaatcaatc gtgtgcgctt ttggcaaaat
    10921 atcaaccaag acccaaagct acaacactat gtaaaagagt attaccgcta tcgtctgctt
    10981 tattcgatga aaaaaatgca gcaaatgcta ttaaacggaa agctgggagc tgtttgtacg
    11041 caactttacg aattatatga tttattcggt gtgcggacag aagaagggat gctgatcgat
    11101 ttttcgatca cgaatgaaga aatcgccaaa ttttgtggca tctcaactgc tagcagtgta
    11161 aacagaattt taaaacaact gaaaaaagat ggtattatca ccgttaaaga ccatcatatc
    11221 gttattttga atatggactt gttagaagac aatattatct tttgatgaac aaagaggttg
    11281 tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
    11341 ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta ccaagaggtt
    11401 gtgagcctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag cttttcatgt
    11461 ttgatgaata ttgtgacttg tgaccgagga gttggtagga gaacaccgtt taccaagagg
    11521 ttgtgaaaaa gctgttctgc atcaagtaat aagaagagcc aagtaaaaac agtctcttat
    11581 gtttttaact tggctggact tatcaccgta gatgtaagct tttgaacgcc gtttatttgc
    11641 aacaaaaggg gctgtgacac gatttttgtc acagcccttt ttttgcgaac attcatttag
    11701 caatttttag aaacttctgc cgccaccgcc aaaagtcccg ccaccgctag aatgggtagt
    11761 acttccgcca ccgccgcttc caggaggtgg gcttttgggg attcttcttg tcgtgacaaa
    11821 agaatttgtc agacgatcgg ttttttcagt taatttgata ctggattttt ctcggaatgg
    11881 atatttgtag atgcctgatt tcaattgata acgcgaaaca gtcacaaaat aaaagacaag
    11941 actaaggata agagccaaaa caacagcaat gatgatttct actgtcgtca aaaccttata
    12001 gcgtgtaatt ttgccagttt cttcgtctac acgataatgt cctcctggaa ctccgtcttc
    12061 cacatatgtt gctgatttag acaggtaagc ttttgcagca ttgtaatagg cagcatttgt
    12121 catctgatca taaacatcat ctaagatcga atcgattctg ctatcagtca aataatcgat
    12181 catattacca gaagtcgaaa tgtagatttc acgctgtccc atatctaata gcaatactga
    12241 gccattttga tcatttccga ctgcgtctct taagtaatta tcagcaaagt ctcttggttc
    12301 ttctgaatta gatgtagtcg ttacgatcaa gactcgtcct ttgatttttt cattcagctt
    12361 ttctgcctgt tgttccaaag attggatttg atctgttgtg aataacccag cctcatcatt
    12421 taccgtagct gtatctgcat gagtagccac cggccagcca acacttacta gcaaaagaaa
    12481 gaataaaaaa aattttttca tcatatcaag taacctccga tcagaaagag gatcgctagc
    12541 ccagcgaaaa tcccaagagc ggtcagtccc aattttttct ggctgatcgg caggacaccg
    12601 ctgacctttc ctgtttgtcc attcattgcg tagtagtaga cttttttact tgagtcattg
    12661 cttcgataag taacaagcca taccggcagt aatacgtaat tatttacttc gtttgtgatt
    12721 gaagcagaag ttcgcacacc ggtcaatgtt gtatatccat ttgcggtatc ttttaacagc
    12781 ttctctgaat agtcttttaa ttcagattga atctgtgatt tgattgcctc atattcgata
    12841 tctctttttt ctgcttggaa cccggctaaa tactggcttt taaaatcaat tgctttatct
    12901 aacgggaaag gttgcacgcc ttcgaccatc ttttgttgcg tattttttga taaggcattt
    12961 tttactagtt ctttaaaaga aagcttgcct gctcttgaaa cagcaaattg cttcgtttct
    13021 gtgtattcga tgtcacctat ccgccatatg cggataactg taccattcgc ctgcatggca
    13081 ccatctactt cagcatctgt agcccaataa gggaaataca cacctgtcaa cttatcgatt
    13141 tgatccttat tgaaaaaagc tttgggaaca aaccattttt tcttggtcca agcgagaaat
    13201 ctgttgattg cttcttcttt ctcgacagca aaaggaagta ctttatttgg caagaattct
    13261 ccgcttaaac gtcccgatag tacgacggga ttatggcagt agtaacaata agtcgcagca
    13321 gttgtcgcat cagtcacgat ctctgcacca cagttgggac aaagaaaaag atctatcgct
    13381 gcggtattat ctgcagattc gtccgctgta tctgaaaaac cgcctgcctc gtcaaccgct
    13441 ttgcgctcgg cttcattcat atcggcaagc tgttcttggg ctgtaaaagt aaaatcttcg
    13501 gtaggtgaag gttcttcttt gctttcttcg ccagccatac gcgcttcttt ttgcttttgc
    13561 tcatattggc tgacttcttc ttctgtatag atattcaggc agtattcgca atgaaatttt
    13621 tggtctttgg gatcaaaagt caacggaccg ccgcaattag gacatttatg tgtaagaaca
    13681 tccatcagct gcacaccttt ctaaataaat taattaacgg ggattccttt aaacggttta
    13741 ccacagtttg gacagaattt agggatgccg ttggaaagat ccacgatctc attgcattca
    13801 ctgcatcgca tttgtagctt aggctgaacg ttttctacag gtttggcagc tccgcagttg
    13861 gaacagaatt ttccggaatt ttctgtcccg caaactggac atgtccaagt atccgcattt
    13921 ccttgtgcag tttgttgatt ttgtttttct gcttgttgtt tgatttgttc ttggttattt
    13981 tgagctgctt gagaaagata agaaccattt gcattcattc ccatacctac acccataaag
    14041 ccggtcattg ctcctgcttc attttttccg gctgcttcca taccacgtgc aatggaacct
    14101 tgcacgtatc cttcacgtac gctaggatca cctagcatcg ccccttcgtt acgcatattg
    14161 atcagtttca cagaatcatc ggtgtaagaa atgctggcta cagccacaga aacaatctcc
    14221 atcccacgta attcacgcca agaatcatcc aatgcagtat ccatgtattt gcttaattcc
    14281 aaactcttag atggtacata agaaatccgt tgaccatctg cagacatctg attgattgct
    14341 gattgtagcg ctgttaggaa ttcagctaaa tactgctcat tgatatcatt gatttccact
    14401 tgtgttttgt ttttagggat tgcgtttgta taaaataaaa tcgggtcaac gacatgaata
    14461 gaataagtgc catgtgcccg taagaataat tctgcgttat agaaattgtc aaagtagttc
    14521 aacggcgcgc ttgtaccgaa tttgattccc tttatttctt ggagattgat ataaaaaact
    14581 tgttgtttct gcggtgtgac gccgccaaat ttgaatcggt caaaagtttc agcaattgct
    14641 tctttcagtg ttccattgaa catggaagga gctgcgtcat ttttaatggt gtaataacct
    14701 tcctcagcgg tataatcgat gatttttcca ccatcgacca aaagcatcat catgtttgga
    14761 tagacatgga cgactgttcc gtctgttaaa acatcttctg ttcctttacg attggagcca
    14821 cgtttgtcat ctttgcggac tttgacacct tttgtcatga ccgttgtatc actcatattg
    14881 tctggttcga tgacttctag ccattgatct gccagaccgc cgccaaccat acttgttgct
    14941 gctttgataa gacccataaa aaattcctcc tctgattcag ctagtgccaa acaattattg
    15001 tttatttatt ataccatagg aagatgtgaa acttctcgga ctagagacgg aattgaaatg
    15061 gaaaattgcg tattgtttaa atacctgaaa aagccgagaa ggatagtagt ttttttacct
    15121 aatatcacgt ataataagag atagtgaatt ttttaaaaaa gggggatcct tatggcatat
    15181 caagcacttt atcgagtctg gcgttcgcag cgtttcgaag acatcgttgg acaaaaagct
    15241 gtgacacaga cactaaaaaa tgctattgtt tcccacaaaa cctcacatgc ctacttgttt
    15301 acaggaccca gaggaaccgg aaagacgagt gcagcaaaga ttttcgcaaa agcaatcaac
    15361 tgtccgaaca gtaaagacgg tgagccatgc aacgaatgcg aaatgtgtcg atcaattact
    15421 gctggtacgc aagaagatgt gatcgaaatc gatgctgcca gcaataacgg agtcgaagaa
    15481 atccgattta tccgtgatcg ggcaaattat gcgcctacaa aggcagcgta caaagtgtac
    15541 attattgatg aggtacatat gctttcaaca ggagctttca atgccttgtt gaaaacattg
    15601 gaagagccaa aagaaagtgt catattcatc ttagcaacga ccgaacctca caaaatacca
    15661 gctacgatca tttcacggac tcagcgattt gattttaaac ggatcaatac ggcagatatt
    15721 gtcgaacatc ttgaatttat cttgaaagaa tcgactatca gctatgaacc agctgcattg
    15781 aatgtgattg cccgttctgc agaaggcggg atgcgggatg cattgagtat tcttgatcag
    15841 gcaatctcgt ttagtgaagg aacaattact ttagatgatg ccatgcaagt gacaggtagc
    15901 ttaactgacg aaatgatgga cagttacttg agtgcttgcg tgaaaaaaga agtaccagaa
    15961 gcattggaaa cattgaacca aattctcgca gccggcaaag aatcacgtcg ctttctagag
    16021 gatatgttac aatactgccg tgacttgctg atgtatcagc aagcacctaa cctagttgag
    16081 gaacaatcga gtacgatcag aagtacattt acagaattgg cacaaactgt ttcagcggat
    16141 cagctgtttt caatgataaa gattttgagc gatactcaaa atgaaattcg ttttaccaat
    16201 agtgccgcta tttatttgga agtcgctaca gtcaaacttg cacagctacc gtctgcttct
    16261 gttccttcag cagatgtagc atttgcagat gatcagatag taaaacagct acaacaagag
    16321 attaaggaac taagaaacga aatcaaggaa ctacgcagta atacaggaat tgagaataca
    16381 aaagtacagc cggtacagca aacaaatcat aaaaaacaga caagtaccta tcgtgtaccg
    16441 acggaacgag tatataaagt tctgcgggaa gcgacaagac agcatctgaa ccaagtaaaa
    16501 gaagtatggg aagacttgct gatgagtcta tctgtcactc agcgagcaat gcttaaagca
    16561 agcgaaccag ttgcagctgg acctacagga ctagtgattg cgtttgatta tgagattgtt
    16621 tgtcaaagag cagcaaatga cgaagaattg cagctttcgg tacacaacaa cttaagccga
    16681 atgatcaaag attatgcgcc tgatcctgtt tatattacaa gagaaagctg gccagaactt
    16741 cgaagaaatt atttgacgca ggcacaagaa gagggaacag aaaactcttt tgcttctgaa
    16801 gactcagaag gagaaatcga gttgcttcct caagaacaag aagtaccaga agaaacactt
    16861 gtagtaaaag cagaggaact gtttggctcc gaagctgtta cagtggtaga tgattaataa
    16921 aaaggagtag ataattatga tgcgtggaat gggcaatatg caaggcatga tgaaacaagt
    16981 acaaaaaatg caaaaagaaa tggcagaagc acaagagctg ctaaataaaa cagagtttac
    17041 aggtaaagcc accaatgaat tggtcaaagc ggtttttaca ggcgatcgtc gcatgaaaga
    17101 tatccaaatc aatgaagaag tcgttgatcc tgatgatatc gagatgcttc aggatcttgt
    17161 cgtaatggca gtcaatgatg cgatcgcaca aattgatgtg gaaactgaga aaaaattagg
    17221 gaaatacgca aaaggtttgc caggatttta gttttaaact gaaggagtaa tgaatatgca
    17281 atatccagaa ccaattgcga aactgatcga cagctacatg aaacttccag gaatcggtca
    17341 gaaaacagcg acaagactcg ccttttatac gatcgatatg aaagatgaag tagtaaatga
    17401 atttgctaaa tctttgttaa gtgtaaaacg cgatttgcat ttttgtagta tctgcggaaa
    17461 catcacagaa gaagatccct gcgaaatatg tagaaatcct tcacgtgatc gaagcgttat
    17521 cctagtggtc gaagagccga aagatgtaat ggcaatggaa aaaatgcgag aatatcacgg
    17581 actttatcat gttctccatg gtgtgttatc accgatggaa gggacaggtc cagaagacat
    17641 caatatttct tctctgttgc agcggctgca tgatgcagat gtaaaagaag tgattatcgc
    17701 aacaaatgca acaacagagg gagaagcaac agctatgtat ctctcacgtc tgatcaaacc
    17761 cgcaggtatc aaagtgactc gtctagcaca tggtctgtct gtaggctctg atatcgagta
    17821 tgcagatgaa gttactttat tgaaagctgt cgaaggaaga cgtgagttat aaatttctaa
    17881 ttaagtgttt tttcagatac aatataaaag cagcacaaga gatgggggca acaatgtgaa
    17941 cggactattt attacaatcg aggggcctga tggtgcagga aaaaccagca tcttgaatga
    18001 actttaccct cttttgaaag aagtagcaaa aacagaaatc attcaaacaa gagaaccagg
    18061 aggcattccg attgctgaag agatccgagc agtgatcctt gatccaaaaa atgatcggat
    18121 ggatgaacgt acggaagcac tgctttatgc agcagcaaga agacaacatc tagtcgaaaa
    18181 ggttctgcct gctctggcaa aagggaaaat cgttttatgc gatagatttg tcgacagttc
    18241 tcttgcttat caaggtgcag gccgacgcat cggcgttacc gagattgctc ggttgaatga
    18301 gtttgctaca gaaggtacta cgccggattt taccctttat cttgacgtag attcagacac
    18361 cggattaaga agaatcaaga aaaacagaca aaatcaaatc gaccgtttag attcggaagg
    18421 tttggaattc caccagcgtg tacgtcatgc ttacttaaag ctggcagaag agaatccgga
    18481 aagaatccac aaagttgatg caagaaaaag ctttgaagaa gtgctgcaaa caagttatca
    18541 tacaattatt gaacagtatc cgcaattttt tgaaaactag gaaggtgaac gtttatgaaa
    18601 attattttag cgattatcca agacaaagac agcaaccgtt tatcaaatga attgattgat
    18661 gcaaatatcc gtgccacaaa actatcatcg acaggcggct tccttaaggc tggaaacagt
    18721 acgtttatcg tgggtattga agatgaccgt gtagaagaag cattggaaat catcaaaaaa
    18781 acgtgtgaat caagaaagca atttgtatct acacctgtta cactggatat ttcgatggat
    18841 gggggcgtcc cttatccagt agaagtagaa gtaggcggtg ccacagtctt cgtcttacct
    18901 gttgaaggat tccatcaatt ttaggaggag ctgagctatg aatcaagaaa taaacttggc
    18961 tcaaatgcaa ccgatcgtgt atcagcagct tcaacggagt tttgagcatg ggcgtatcgc
    19021 tcatgcttat ctttttgaag gggagaaagg aacaggaaaa catgaagtag gtatatggct
    19081 tgcacagcac ctgttctgta cccaaatgaa agacaatctg ccttgtggaa aatgcaacaa
    19141 ttgtcagcgg atcgaaaaga aagaacatcc agatgttttg gttattgaac cggaaggaca
    19201 aaccatcaaa gtcgatcaga tcagacgttt gcagacggaa ttcagtcgca gcggttacga
    19261 atcaagaaaa aaagtttttt tgatcaaaga agctgaaaaa atgaatagca gtgcggcaaa
    19321 cagtttgttg aaattcttgg aagagccccc tggagatttt ttagcggtgt tagaaacaga
    19381 tgcagtagga agaattctcc ctactattca atctcgttgt caagttctgc attttcaaga
    19441 actttcgaaa gaagcactga tccatcgatt gcaagaagag cagattccat tggaaaaagc
    19501 aaaacttttg gctttcttaa caaacagttt aggaaaagca gttgaaatct ctcaagatga
    19561 atggtttaat gatgctaagg acttgattta tcaatggttc gtttacttgc aaaagcaaga
    19621 tactcaagcc tttatctatg tccaaaaaaa attggtaaaa tcatttaaag aaaaaacaca
    19681 acagtttaca ggattatctg tcttgatgtt ttattatcag gaagcacagc gtcaagcagt
    19741 agcaaacggc gaatatggca aagtgaaaca gataaaccag ataattgaac ggatattgat
    19801 cgcagagcag aaactacgca gcaatgtcag ttttcaggct gttgcggaac agttcgtttt
    19861 gcatacgatt ttttagcttg ccgcaaagga tgatgatcaa ggaggatgga aatggtagaa
    19921 gtagtaggtg tccgatatag agatgcaggt catatctatt attttgcacc aggagactct
    19981 gaatatgttt atagtgacaa ggtattagtc gaatctcagc aatccttaca tattgctact
    20041 gttgtccttc ctaaaaaaga gattgatccc aatgaccttc ctgaagctat ccgtccgatc
    20101 ttgcataaag cgtcacaagc tgaattagaa aaagacaaaa aaaacaaaaa agatgcttat
    20161 gatgcattga aaatcgcaaa agaaaaagtg cagttgcgtg agttggaaat gaaaatggtc
    20221 aaagccgaat acacatttga tcgaaataaa ttgatttttt ctttcacagc ggacgggcga
    20281 attgacttta gagaattggt aaaagatctg gccgctgtat ttcgtactcg tattgagctt
    20341 cgacaaattg gtgtccgcga tgaagcaaag ataataggcg gaattggtcc atgtgggcgt
    20401 ccattatgct gttcttcttt tttaggcgat ttcgtgcctg tatcaatcaa aatggcaaaa
    20461 gatcaaagtt tatctttgaa tccaacaaaa atttccggtt tatgcggacg cttgatgtgc
    20521 tgtctgcaat atgagagcga agaatacgag acagcgaaaa aagaactccc tgactttgga
    20581 aaagtaattg ctacacctga tggtaaaggg aaagtcgtag ggttgaatct gcttagccgt
    20641 atcgttagta ttcggctgac tggacgggaa aatcctgtcg aatatgaatg ggatgaattg
    20701 aaagaattta tccaagtagg tgagatgaat ggataagaga tcattatacg acggattgaa
    20761 tcaattagaa tcagagttga gaaatacatt aacagaactt gttgaaatga agcatgcttt
    20821 gcatgagatc gtagaaaaaa atacaacttt ggagatggaa aatcaacgtc tgcgggaaca
    20881 cctgcgggaa ttgaaccaag ttgcacaagc tccagctgaa aatgtgaagc aggaactttc
    20941 taagtctcgt atgaatcttg aaaaaattta tgaagaagga ttccatgtct gctacgattt
    21001 atatggatca agaagggaca atgacgagcc ttgtgcgttt tgtctagaag tgatctatcg
    21061 cgaaagcggt cgatgaccaa taacaagagg agactggagc ggagaaatac tgctccagtc
    21121 tttttattaa ggagattgtg acagatgtat aatcaaaaaa gtttcaaggg aaatccggta
    21181 tatggggatt tatatcttgt accgacgcca attggaaatt tagaagatat gagctacaga
    21241 agtgttcgta tgctgcaaga atccgatctt atcgcaagtg aggatacgcg taatacacaa
    21301 aagttattga atcattttga aatccataca ccccaaaaaa gtttacatga acataattat
    21361 aaagaacgtg tccctcaatt gattgctttg ttaaaagaag gaaaaacgat tgctcaagtc
    21421 agtgacgcag gaatgccttc catcagcgat cctggacatg agctagtttt agcctgcata
    21481 aaagaaggga tttctgtgat ctctatccca ggacctacag ctgggatgac ggctttgatt
    21541 gcttcagggc tagtgcctca accattttta ttttatggct tccttccacg taagaaaaaa
    21601 gaacagaaag aaacattgaa aaagctaaaa gaacagacag ttacgctgat tttttatgag
    21661 tcaccttatc ggatcactgc tacattgacg aatttcttag aaatttttgg aaatcgccaa
    21721 gtcgttttgt gtcgggaatt gacaaaggtc catgaagaat accttagagg aagtacggaa
    21781 gagctactcg cttatatcga agaacatcca gtaaagggtg aatgttgtct gcttgtagaa
    21841 gggaatttgt tcgaaatgcc agtgacggaa gttgatgaag agttgggaac gttaaaggaa
    21901 caagtagaag caaagatcgc ttcaggcgaa aaaccaaatg ctgctatcaa agcagtagcg
    21961 ctgaaaaatg ggctgaaaaa acaagaagtc taccgtcagt accatgaatt agactgagat
    22021 agtataagtt gtctataatg aaagactaat gaggaaatca gctactaact tttcaaatct
    22081 cttgaacgcg aacgaatgtt cttgtataat gaataagagg tgaggatgat ggagctgcaa
    22141 tttcctttac ataatgatac atcaagaaaa atcattcata ttgatatgga tgcttttttc
    22201 gcttcggttg aagaacgaga tcatcctgag tttcgcggac atccattagt aattgccaga
    22261 caccccagtg acactggtgg gcgtggagta gtgactacag caaattatga agcaagaaaa
    22321 ttcggaattc attcagcaat gagtgcacaa aaagcgtatg agctttgtcc gcaagccatc
    22381 tttaagcctg gcgaccatca taaatacagc gatatttcca aacaagtacg ggaagtattc
    22441 tatcgttata cagatattgt ggaaccagtt agtatcgatg aagcgtatct tgatgtgacc
    22501 gaaaataaaa tcaattgcaa gtcagccatc aaaatcgccc gaatgatcca agcagatatt
    22561 tgggagagtg tccagctaac atgttctgcg ggagtaagtt acaataagtt tttggccaaa
    22621 ttagcatcag actatcaaaa acctagagga atcacggtag ttgcccctca agacgctgtg
    22681 ccatttttaa aagctttgcc aattgaaaaa tttcatgggg tagggaagaa aactgtacct
    22741 agaatgcatg agctagggat ttatacagga aaagacctat atgaatgcac agaaatgatg
    22801 ttgattcgaa attttggaaa aatgggctat tctctttatc gtaaagtcag aggaatccat
    22861 gattctccag taaacgttac aagagagcga aaatctgtgg gaaaagagca tacttatggg
    22921 cagccgttgc aaacggaaga agcagtgctg actcagttgc gccaactagc tgaaaaagta
    22981 gaagaagcac tgagaagagt ccaaaaacat gggaaaacag tagttttgaa ggtcaggtac
    23041 acggactatt ctactgtgac caaaagagtg acattgccag aatatatcta taaaaaagaa
    23101 gcactctttt atcaagcaag ccttatatgg gaggaaatct tgggagtaga aaaaggaatc
    23161 cgattgttag ggattacttt gacgaatctg gacccgatga cttatgaaaa catcgtcctt
    23221 ccattatggg aaaatcaaga aatataaaaa cagctttgag agttgaaacc ggaaatccgt
    23281 tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc ataggtttct
    23341 gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga tttttggacg
    23401 tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg tttgcttaat
    23461 tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt atcttctgaa
    23521 tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt tgtcgtaccg
    23581 aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa aaaaggttcg
    23641 ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat gatttgttct
    23701 tctaattcag gtgtgaatgg tttgccatat cggaaatttt gtgcagcttt gttgaagcag
    23761 ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc ttcaccatcg
    23821 acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt tgctaaccat
    23881 tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca aagatatagc
    23941 tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta aatccattgt
    24001 tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc catcgaaaga
    24061 gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt tcaaaagctt
    24121 gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt tgtttagcta
    24181 ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt ttcacacgtg
    24241 aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt cctaaatagc
    24301 cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat tctggacatt
    24361 cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag caatgatcga
    24421 ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat acagcttcta
    24481 aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat ccacctgaac
    24541 aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg tttttacgca
    24601 catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg atgatgccga
    24661 atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga gttgaataga
    24721 cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt aattctttga
    24781 cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca ccgaagaaag
    24841 ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga cggtttttga
    24901 ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca tacatatata
    24961 aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag agaagcgcat
    25021 ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga tctccttttg
    25081 attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt cgtccgacat
    25141 ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag cccataggta
    25201 ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca ggatacattc
    25261 ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct tcagggttta
    25321 agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc cgcttttcac
    25381 tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt tcaaaccagt
    25441 tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa gtaaataagg
    25501 tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg gtttttgctt
    25561 ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct tttagatgtt
    25621 ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg gcagaacagc
    25681 caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct gtggcggtct
    25741 ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg ttcagcatgc
    25801 ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa tccaaatcat
    25861 ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac attttcagtc
    25921 cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa acattactgt
    25981 ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt tcaatcgaaa
    26041 agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa ttgatgtact
    26101 cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccgcgatg ttttgaatct
    26161 catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc aagtctgcaa
    26221 tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc ttttctgctt
    26281 tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca tcgcgtttga
    26341 tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct tcataattta
    26401 ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca aaatcgggga
    26461 aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt ggatttctat
    26521 actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct ggtggatttg
    26581 ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt attcagtcca
    26641 agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta tgcaaataaa
    26701 tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa gtgaaacatc
    26761 taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat atctcattca
    26821 cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga aagacaacgc
    26881 tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg atccaaggaa
    26941 aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa aaactaggat
    27001 tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat atcctattgc
    27061 ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga catgtagctc
    27121 aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct gtcggacaga
    27181 gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt tttgcagatg
    27241 aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac ttgaagacca
    27301 tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat acagcaagct
    27361 actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt gtcagaagag
    27421 ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt ggcggaggag
    27481 gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc acgaggctat
    27541 cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt cagcgcaatg
    27601 acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga tggtggattg
    27661 agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag tgccaaccga
    27721 tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg tatgaataaa
    27781 ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt cgcctgtatt
    27841 tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt ggttcgaatg
    27901 atggatatgg atcttactag tccatttttt atctccattc catcagtagt agatacttta
    27961 tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa aatatggcgc
    28021 tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac aatgcgcttc
    28081 aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag cggatatttt
    28141 ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta tcttgataca
    28201 ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac ttatttgttt
    28261 ttctgttata catttcggtt atttttgatc atattgagta aatggaagat ttattactat
    28321 ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag ttggcgtaca
    28381 aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg cacggctagt
    28441 atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta tcagtttgat
    28501 gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa gataactaaa
    28561 gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct cgaagaacct
    28621 atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag aaaagttaat
    28681 ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct tgatcaaatg
    28741 caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc agataatcaa
    28801 gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat ggtatatcta
    28861 agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt ggattatcaa
    28921 gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg actgagcaat
    28981 gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt aaatggaagt
    29041 ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga agcacaattt
    29101 agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg tgcagttaat
    29161 cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt gattattgct
    29221 actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa agaaaattat
    29281 cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat cttcgagcaa
    29341 aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt atttgcaatc
    29401 aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct tgtgagtggc
    29461 ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata ttgtcggatt
    29521 atcgaggaat gatatacg
//
LOCUS       DAZKNO010000013        28380 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000013 DAZKNO010000000
VERSION     DAZKNO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.013"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG8MY_000422"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="ACG8MY_000422"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0429653.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="ACG8MY_000423"
     CDS             complement(937..1518)
                     /locus_tag="ACG8MY_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0429654.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG8MY_000424"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="ACG8MY_000424"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0429655.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG8MY_000425"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="ACG8MY_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0429656.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG8MY_000426"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="ACG8MY_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0429657.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="ACG8MY_000427"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="ACG8MY_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0429658.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="ACG8MY_000428"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="ACG8MY_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0429659.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG8MY_000429"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="ACG8MY_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0429660.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="ACG8MY_000430"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="ACG8MY_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0429661.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="ACG8MY_000431"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="ACG8MY_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0429662.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="ACG8MY_000432"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="ACG8MY_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0429663.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="ACG8MY_000433"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="ACG8MY_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0429664.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG8MY_000434"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="ACG8MY_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0429665.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="ACG8MY_000435"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="ACG8MY_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0429666.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG8MY_000436"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="ACG8MY_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0429667.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="ACG8MY_000437"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="ACG8MY_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0429668.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG8MY_000438"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="ACG8MY_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0429669.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG8MY_000439"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="ACG8MY_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0429670.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="ACG8MY_000440"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="ACG8MY_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0429671.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="ACG8MY_000441"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="ACG8MY_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0429672.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="ACG8MY_000442"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="ACG8MY_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0429673.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="ACG8MY_000443"
     CDS             13972..14157
                     /locus_tag="ACG8MY_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0429674.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG8MY_000444"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="ACG8MY_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0429675.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="ACG8MY_000445"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="ACG8MY_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0429676.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="ACG8MY_000446"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="ACG8MY_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0429677.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG8MY_000447"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="ACG8MY_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0429678.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG8MY_000448"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="ACG8MY_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0429679.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="ACG8MY_000449"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="ACG8MY_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0429680.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="ACG8MY_000450"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="ACG8MY_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0429681.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="ACG8MY_000451"
     CDS             18232..18879
                     /locus_tag="ACG8MY_000451"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0429682.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="ACG8MY_000452"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="ACG8MY_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0429683.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG8MY_000453"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="ACG8MY_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0429684.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG8MY_000454"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="ACG8MY_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0429685.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG8MY_000455"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="ACG8MY_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0429686.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="ACG8MY_000456"
     CDS             20317..21255
                     /locus_tag="ACG8MY_000456"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0429687.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG8MY_000457"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="ACG8MY_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0429688.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="ACG8MY_000458"
     CDS             21935..22774
                     /locus_tag="ACG8MY_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0429689.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="ACG8MY_000459"
     CDS             22750..23619
                     /locus_tag="ACG8MY_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0429690.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="ACG8MY_000460"
     CDS             23609..24406
                     /locus_tag="ACG8MY_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0429691.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="ACG8MY_000461"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="ACG8MY_000461"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0429692.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="ACG8MY_000462"
     CDS             complement(25688..28357)
                     /locus_tag="ACG8MY_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0429693.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         9210 a         5282 c         6037 g         7851 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aagggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
//
LOCUS       DAZKNO010000014        27986 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000014 DAZKNO010000000
VERSION     DAZKNO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.014"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(473..2365)
                     /locus_tag="ACG8MY_000463"
     CDS             complement(473..2365)
                     /locus_tag="ACG8MY_000463"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429694.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2362..4089)
                     /locus_tag="ACG8MY_000464"
     CDS             complement(2362..4089)
                     /locus_tag="ACG8MY_000464"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429695.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4105..4755)
                     /locus_tag="ACG8MY_000465"
     CDS             complement(4105..4755)
                     /locus_tag="ACG8MY_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0429696.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4959..5255
                     /locus_tag="ACG8MY_000466"
     CDS             4959..5255
                     /locus_tag="ACG8MY_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0429697.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5447..6067
                     /locus_tag="ACG8MY_000467"
     CDS             5447..6067
                     /locus_tag="ACG8MY_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0429698.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6159..7325)
                     /locus_tag="ACG8MY_000468"
     CDS             complement(6159..7325)
                     /locus_tag="ACG8MY_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0429699.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7337..7690)
                     /locus_tag="ACG8MY_000469"
     CDS             complement(7337..7690)
                     /locus_tag="ACG8MY_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0429700.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7818..8840
                     /locus_tag="ACG8MY_000470"
     CDS             7818..8840
                     /locus_tag="ACG8MY_000470"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0429701.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8859..9020
                     /locus_tag="ACG8MY_000471"
     CDS             8859..9020
                     /locus_tag="ACG8MY_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429702.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9279..9713)
                     /locus_tag="ACG8MY_000472"
     CDS             complement(9279..9713)
                     /locus_tag="ACG8MY_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429703.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9823..11682)
                     /locus_tag="ACG8MY_000473"
     CDS             complement(9823..11682)
                     /locus_tag="ACG8MY_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0429704.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11683..11823
                     /locus_tag="ACG8MY_000474"
     CDS             11683..11823
                     /locus_tag="ACG8MY_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0429705.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11800..12959)
                     /locus_tag="ACG8MY_000475"
                     /pseudo
     CDS             complement(11800..12959)
                     /locus_tag="ACG8MY_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(13266..14690)
                     /gene="araA"
                     /locus_tag="ACG8MY_000476"
     CDS             complement(13266..14690)
                     /gene="araA"
                     /locus_tag="ACG8MY_000476"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0429706.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14706..15404)
                     /locus_tag="ACG8MY_000477"
     CDS             complement(14706..15404)
                     /locus_tag="ACG8MY_000477"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0429707.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15361..17022)
                     /locus_tag="ACG8MY_000478"
     CDS             complement(15361..17022)
                     /locus_tag="ACG8MY_000478"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0429708.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17240..18640
                     /locus_tag="ACG8MY_000479"
     CDS             17240..18640
                     /locus_tag="ACG8MY_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0429709.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18892..19971)
                     /locus_tag="ACG8MY_000480"
     CDS             complement(18892..19971)
                     /locus_tag="ACG8MY_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0429710.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20057..21016)
                     /locus_tag="ACG8MY_000481"
     CDS             complement(20057..21016)
                     /locus_tag="ACG8MY_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0429711.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21028..22539)
                     /locus_tag="ACG8MY_000482"
     CDS             complement(21028..22539)
                     /locus_tag="ACG8MY_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0429712.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22561..23124)
                     /locus_tag="ACG8MY_000483"
     CDS             complement(22561..23124)
                     /locus_tag="ACG8MY_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0429713.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23130..23993)
                     /locus_tag="ACG8MY_000484"
     CDS             complement(23130..23993)
                     /locus_tag="ACG8MY_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0429714.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24007..24903)
                     /locus_tag="ACG8MY_000485"
     CDS             complement(24007..24903)
                     /locus_tag="ACG8MY_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0429715.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25078..26367)
                     /locus_tag="ACG8MY_000486"
     CDS             complement(25078..26367)
                     /locus_tag="ACG8MY_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0429716.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26339..27856)
                     /locus_tag="ACG8MY_000487"
     CDS             complement(26339..27856)
                     /locus_tag="ACG8MY_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0429717.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8184 a         5882 c         4850 g         9070 t
ORIGIN      
        1 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaaaagtcgc
       61 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tctacatttt tgcataaatt
      121 tggcttaata ccgagaaact ggcttttgaa caccgtttac caagaggttg tgagcctgcc
      181 gtttaactcc gagtcacaag gagcaatttc aaaaaacagc ttctcaggtt tgttgactat
      241 tgtgacttgt gaccgaggag ttggcaggag aacaccgttt aaggaagagg ttgtaaaatc
      301 agcggtttga cctgagtatt agagatacaa aacggaaaaa tagcttctca tattttgccg
      361 ttttttgagc taataccgaa ggacagctga ttgaacaccg tttattcgga aaaagggact
      421 gtgacacgac ttttgtcaca gcccctaaaa aaaataataa atttgtacta ttctatgaag
      481 cttcttcttc agaaaactga ctgttataaa gatctgcata gaatccgtct ttttccatca
      541 atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt catcacaata atgttgtctg
      601 catcgcgtat tgtagacaag cggtgagcaa caacgaaact tgtccggttt tccaataggc
      661 ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc tacactagat gttgcttcat
      721 caaggatcag gacatctgga tttgctaaga atgcccgcgc aattgtgatt agctgacgct
      781 gcccttgcga aatattgctt gcatcctcgt ttaatacagt gttatatcct tctggcaatt
      841 tacgaacaaa atcgtctacg tgagcagctt tagcagcctc aatgatttgt tcgtctgttg
      901 catcgtcgtt cccatatttg atattgtcat agatacttcc tgtaaacagc caagtatctt
      961 gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga aagatcacgg gtatctacac
     1021 catcgtaacg aatactacct ccagaaacat cgtagaaccg ttcaagaagg ttgatcagtg
     1081 tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat ctcaccaggt ttgacatcca
     1141 agttaaaatc agtcattagc aagttatctg gtgtgtagcc gaattgaaca ttttcaaaac
     1201 gaactttgta tggtgtatct ttttcttctg gaaggccaga tttttcattg gacatctctt
     1261 cttcatccaa cacttcaaat acccgttcgg ctgaagcaac tgttgattgg atagtgttca
     1321 tcaaacttgc aagttgagtg attggttgag agaactgtgt tgtatattgc aagaacgctt
     1381 gaacatcccc taatgtcatg ttcccatttg caacttttac tccgcctagt acagcgacga
     1441 acacatagcc tagattttta acgaaattca tcaacggcat gatgattgcc gaaataaatt
     1501 gagctttcca accagcggca tacagattct gattttcttt ttcaaatact tcctggtctt
     1561 tttcagtatg attgaatgtt ttaacaatgg tatgaccacc atatgtttct tctacttggt
     1621 tattcaataa acctaaactt ttttgctgat ctgcgaaaaa cacttgtgat ttcggcgcta
     1681 cgatcatcac gatgatcaag cttaaaggga tcatcaataa cgcgactaac gtcaattgcc
     1741 agctgattga aagcatcatc acgatgaccc caatcaatgt gactgcacta gttacaaatt
     1801 gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat atcattgatt gctcgagaca
     1861 tgatatcccc atttgtatgt gtatcataat aggaaatcgg tacacgattc attttggctt
     1921 ccaaatcatg acgtaattca taaactgttc gttgggaaac acgtgtcata ataaattgct
     1981 gtaaaaagtt gaatacagca gaaatcaaat acatcacgat tactgtcgca atgatctgcg
     2041 cgattttatc aaagtctatc ggaaattttg ttacttgttg gccttgttgc atctgttgag
     2101 cgccagccat tacacctttg aatatttctg tggttgcttt ccccaagacc tttggtgttt
     2161 ggatttggaa gatcacggct gctattgcta gtacaaatac agcgatgatc gcaaccatac
     2221 gtttagacat atagccaaac aatcggcgta ccgttcccca gaaattttta ggcttttcac
     2281 cttttacacc gagatttctg ccaggtccac catgtcctgg accgcctgct ggtccatgag
     2341 agggacgtgt gtttgtttta ctcatgctaa gtcctcctct cttagttgtg aatgaacgat
     2401 ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt gttcctttgc caaccagttt
     2461 tccttcatct agcacaagga tcatatctgc atccattact gtacttactc gttgagcaac
     2521 taaaacggtg atactttctt tcatattcgt tttcaatgct tggcgaagat tggcatccgt
     2581 tttgaaatcc aacgctgaga aggaatcatc aaacacatag atatctgctg ttttgactaa
     2641 tgcccgtgca attgccagac gctgtctttg tccaccagag aagtttcctc ctccttgttc
     2701 cacatggcta tctaagccat cttccatctc tgaaacaaaa tctttagctt gtgcgatttc
     2761 taatgctttc caaatcattt catctgttgc atccggctta ccatactgca tattgtctcg
     2821 gatcgttcca gagaacagaa ctgctttttg aggaacaaag cccattaatt ctcgcaaatt
     2881 atattgagtc atgtcactaa tgtccgttcc atttatttgg atcgaaccag attcgatgtc
     2941 atataatcga gggatcaaat tgactaaggt cgttttacct gaaccagtcc cccctatgat
     3001 tgcgacgatc tcgcccgact tggcttggaa atcaatatct tccaatgcaa gtttttctgc
     3061 tccatgatat cgataattga catgattgaa ggccaatgtt gcgttttttc cagcaaaaga
     3121 aactgtttta ggattttcag ggtcatggat accgattttt tcatccaaca cttcattaat
     3181 acggtcagct gaagcttgtg cacgaggcac catgataaaa atagcggaaa gcatcataaa
     3241 gctgatcaag atctgcatcg cataagtcat aaatgcaatc agattaccga cttccaaagt
     3301 catatctgct atgtagtgtc caccaaacca agtgattgct acgtttgtac cgctcatgat
     3361 caatgtcatc attggcatca tcaatgcgac gatcgtatta actttgattg ccgtatttgt
     3421 ataatcttta ttcgctaaat caaaacgatt ttcttcaaag cgtgtcttat cgaatgctcg
     3481 aatcactctg acccctgtca atccttctcg aaataccagg tttaaacggt cagttttctt
     3541 ttgcatactt ttgaataatg gaacagcaaa gtacatgatt ccgccgatca aaataatcat
     3601 tactgggatg acaactaaga agatttttgt caactgatga tctttttgat aagcaagtat
     3661 gctcgcgcca atcaacgtga ttggtgcatt gatcatcata cgtaaaaaca tttgtgtaac
     3721 catttggatt tgagtcacat cattggtcgt tcttgtgatc aaagaggccg ttccatattt
     3781 atcaaacgca tcttttgtca gactctctga ttttttatag acatccgtac gcaattgctt
     3841 acccagtttc tgtgattcac gagtagcaaa aaatgtattt gcgatagcag ctaagatact
     3901 gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa atataatcga catctccttg
     3961 cgctacccct ttatcaataa tattggaggt aagtgtcggc aaatataaat ctgccagtac
     4021 ttggatcacc atgaaaactg cagctgcaat tgctgaagtc aaggagatcc gttttacgat
     4081 ttttagcatc tagttccctt ctttctattt atatactccg tttttcagcc aagaaagttt
     4141 caaattgaat tcactgattg cttgctctac tgagtaatct tcagatgttg aaaataagcg
     4201 atacgaatgc gcaattgttg taaatacagt attcatcact aattggatca gtaattccaa
     4261 ttcatgatca ttttttactt tcagcttcgt ccgatcgatc aattcaagca actcttttgc
     4321 atcttgtttg tgttctttat gtggatcgtg aggcgttttt tggcgactcg tatgaggagc
     4381 gacttttctt gaggcacggt aatccatgtt cataaacaag ttacgataaa aagaagcatt
     4441 ctcgccttcc aaaatctcat aaatcatttt agaaaaatag gattcgaatc ctttgaatag
     4501 atcaccatcg gattctttca ttagttgtaa catatcttta gagctgtcac gtctcattgt
     4561 ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa tactgataga aactgcctct
     4621 agggatatcc gctaatttga cgatattagc aatcgaagct tcttttaagg gtactctaga
     4681 aaactcgatt ctcgcagcct cgattagtct tttttgtttt tctttaggca aatggaaaaa
     4741 tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg acacattgtc atatgacgct
     4801 atgtcattat aacacctcct acctgttttg caagacattt gcatagtttt tttcattatc
     4861 atccgataca ttaaaaaagt gataagagga atccttccat ttgaattaat gagagttgcc
     4921 cattaaaata gccacatact aaacaaagga tgagataaat ggcccaatca attagtgtcc
     4981 aagacttcaa taaattgcct ctcgatgctg acagcatcgt tttagataca agaaatacct
     5041 tagattacac aaaaaaccac ttaagccact cgctttctat ccccgctgct atcttgccaa
     5101 gacaattaaa aaatctagat ccaaagaaac tttattatat tctaagctat acaggcaggc
     5161 gatcagaagc attagccgat caacttactg ctaatggttt tcgtgctgtt tatatcattg
     5221 gtgggatgga agctcttcag tcgagtgttg cttgaatatt gcttgttaga ttcaaaagag
     5281 gctgtgacaa aaaatcgtgt cacagcctct tttataccct gtcttttttt agttatttat
     5341 tcttcaacct tatcaatatg acttgatagc cttttctttt tcgagtaaac tgattttgtt
     5401 agtcatagtt ataacaatca tagcaataaa aggagaaggt aagcatatgg catctgtttt
     5461 agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc acagttaagg cattgacgtc
     5521 ttttttaaca agttataaag aaaatcatcc agatgatgaa gtgactgttt tggaacttta
     5581 tcgcgatgat attccggaaa tcgatgaaga attattatca ggttgggaag cattacgtgc
     5641 tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa aaaattgctc gtttcaatga
     5701 attgactgat cagttccttg ctgctgataa aatcgttatc gcaaacgcct tatggaatct
     5761 aaatatccca acaaaattaa aagcttggtt cgataccgtc aatgtagctg gaaaaacgtt
     5821 ccgctataca gaaaacgggc cagaaggatt agtgacaggt aaaaaagcac tgcacatcca
     5881 atctaatggc ggtgtataca acggacaaga ctttgcctct caatatgtaa aaggtatttt
     5941 aaactttgta ggcatcgatc aagtagatca gctattcatc gaaggaatcg attatgaccc
     6001 tgaccgtgca gatgagttga tgcaaaatgc attagataaa gctgctgctt taggtaaatc
     6061 attttaaaaa ttgagataaa tgcatacaaa agagacagag gaaaaaatct tcaaagcagc
     6121 atttgtgcct tggatatttt cccatctgtc tctttttgtt attctttctg ctttttcaac
     6181 ggattttctt cataaagaga agagattgtt gtgtaccatt caaaaacatg agtaatcact
     6241 actttagctg ctgcataaac aggaatcccc aagatcacac cgatgattcc gaacaatttc
     6301 ccagaagtca gcagaactaa taaaatcgtt actggatgga ttgctaactg attccctagt
     6361 actaatggag agatcaagcg tccttctatt gtctgctcta ctgcaaaaac gatcagtact
     6421 ttgatgatca tgaatggacc tgctacaatg gctaaaaaga tagccggaat catcgctaaa
     6481 aatgatccta agtaagggat caggtttaaa aaacccgcaa taatccccaa cgtgacagca
     6541 tactctaaac caatgaccgc aaaaccaatc ataaacatga ttgccacagc aaaagccaca
     6601 gttagttgtc ctcttatata agaagaaact tgactattca tttcactcag cacattcaac
     6661 gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat aaggtgctaa ttgtttaccg
     6721 tctttcaata gataaaataa gataaacggc atcgtcagca gagccactaa aatagtcgct
     6781 accgcgccga caaaacttcc aagactctga actgtactct tagatatatc ttggatcata
     6841 tcgcctgccg aattcatgaa cttatcacct gctgtttcca actgttcgcg aaattggttg
     6901 aacagcgggt cccgcaaaat ctcggttaat ttattatcaa tcgtatctac atattgaggg
     6961 aagttactga tgaagctcac tgtttgttcc tgaatcttag ggatgatcac tacgctcccc
     7021 catgcgatta gtgcaacaac caaaacaaac agcccaatga tactgtaaag ccttggtact
     7081 tttcgctttt caaagtaatc aactaccgga ttcatcaagt aatacaagat tcctgctaaa
     7141 atgataggca gacctacgat tgctaaaaat tgccagattg gtgcaaacaa atatgaaact
     7201 tttgtaaata aaaagacgat cagcaatatt aacaatacaa caagtaaggc tgtgactact
     7261 tgattattta aaaaccagcg ccaaaaccac gataccggtc tgttcttctt attttttcct
     7321 tccataattc ctcctgttag ttataaaaaa tttggcttcc caaaccaata gcaggcagtc
     7381 tatgcggatg aagatagatt ccatttgcaa tttgtccttc ttctggacaa gcatcaaaaa
     7441 tcaatgatac gtgagctact gtctttaaat tcccattggc catcggaccg acataatcga
     7501 tcgtatatac ctgatcatca aaagcaattg ttccgcctgc tttcaaagaa aaaggttcgt
     7561 cttttgatac ttcttgaatc accgaatatt ttcgtaaggc agctgttgct gttttatcga
     7621 acaaaatcag catgggttct tccttgttta ttgcttctgt cccaatttct gtaatctttg
     7681 cctgaatcat tgcactctct cctttaattg ctttcaaaaa cagtataaca cattctcatt
     7741 ctttagccgt gtggaataat tatgtgatag aataaaaacg aaaactattt cattacaatt
     7801 aggaggaatc agctcatatg atcgaaaaga taattttagg aacctatacg cgtcgcgaaa
     7861 gtaaaggaat ctatacgatc gaccttgata cagaaaaaga agaattagcc aacctttcat
     7921 tagctgttga agaaaatagc cctacctatg tagcaaaaag taaagctggc aatctttaca
     7981 cagttactag tgtggatgga ttaggtggtg ccggtgcgta cgataaggat tttcattttt
     8041 tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt agcggttgat gaagcacgtc
     8101 aattactata cggggcgaac tatcataaag gtgaagtaaa tgtctaccat atattaggag
     8161 atggcgaact gaaagcagca gattcaatat tccatcaaga agcgacaggt cctcataaaa
     8221 accaagatca tgcgcatgtc cactatacgg atctgactcc tgaccaacga ttagtcgtat
     8281 gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc cgaaaacggc aaattgaatc
     8341 ttgtaactac cttcactgca gagccaggaa caggccctcg ccatcttgtc tttcatccaa
     8401 atggacaatt cgcttattta tttggtgaat tagacagcac agtccgcgtg ctttcttata
     8461 ataaagaaac aggtgctttt gaagaattac agaaaatatc gaccttgcca gaagaattcg
     8521 acggtgaaaa tggaggggca gctatccgca tttcaaatga tggcaagttc ctttatgctt
     8581 ctaaccgagg acataactcg attgctgtat tcgcagtttt tgaagatggt tcccacattc
     8641 aaaacaagca aatcatctca acagaagggg attttccccg cgatttcgca ctagatccaa
     8701 gcaataattt tgtcgtgtgt gccaatcaga acaccgataa tctgacatta tatcgccgtc
     8761 atcctgaaac aggattactg gaattactga aaaaagatgt ctatgtacca gaatgtgtct
     8821 gtgtttattt tgatcgttag taagaaaagg agaatgaaat gaatcagcaa gacgtattag
     8881 acagactacg ggaagaacta aatattcctt tctttgatgg gaaaatcgaa gataaagagt
     8941 atagcgaaga ggaatatcag aaactcaaag ccgatttaca gaactacttt gaacaatatg
     9001 tacagaatgt agaaaactaa tcagctttcc atactatctg aaaagaacat caggacaaga
     9061 cttttccgtc tatcctgatg ttcttttcta caaataaaaa accgccgaga tggttagact
     9121 cgacggaaaa ggagtattta ctcgattttg agtaagtaca aggttgttgt tggttatagg
     9181 tatgtcatta tcataaagga ttttgatcac tctatcataa aggtaatatt acatttatat
     9241 tagataactc tagtttttat tgttatcaaa gatttctttt attttttcaa caacaaatta
     9301 gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt ggtcttgttt tgcttcagat
     9361 gtattgaata gatcttcatc tacccctgat tcaagatcga tactttggat catttcttca
     9421 cagcctgcta catatgaaag ataagctttt tcaaaatttt tatgaatgcc cattgctttt
     9481 gctggtggac gcaaagtact gactttcata agcattgccc gatatttttt tgttccttct
     9541 tcaaataaca ggatgatctc ttcaagagtc gatttgtcca gttcacttgt ttttccttca
     9601 tcaattgcgt ttctgatcac ttcatatttc ggatgcattc cttctccgat tgcttctgtt
     9661 tccttgacga tttgatcgat ggactgcaca taatagccaa tattcggtaa catgttcgtt
     9721 ttcctacttt ctccattgtt gttattattt attctacttg tttttatcct caaatgacaa
     9781 gtacttgaaa aaatttaatg gtttctatac agctcgccat tcttattcgt gctcaaagaa
     9841 accatctgct aaactatatt gtttccctgc gacaatgtca tattggatca attgatattc
     9901 gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct ttataccact gtccgttttg
     9961 atagtacagt tctcgttcaa acgccggaag ttcctgttct aatgataaaa gcagctgata
    10021 aaagccattt gttgtctgat tcgtctgttc cattagattc gctgcaaaat aaaacggact
    10081 ggtgattgca tcgtgagtcg tttgcttttc taattctcct ttactgtcga acataagaaa
    10141 ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg atcgtatctg aatagatccc
    10201 tggaagatga tccccccaaa aaacaactaa agtccttcta gaaagattct tcaattcttc
    10261 cgtgaactgc ttcagtgccg tgctgctata ggaaatatct tgcagataat tctctaagtc
    10321 aagtgtccca cttccatctg aagtttttgc aggatagtct agattatcat actttccatc
    10381 gtagggcata tgtgtctgca tcgtgactaa atgaatgaac tgaggtgttt tttcttcctt
    10441 gagaagtgtc attacttctt tgtaagctga ctcatcagaa atatatggat tattctctat
    10501 cgtatccgta taggtcatcg tacgctcgct gatgaactgg tcaaatccta atgtctgata
    10561 aacatcctct cttttataca tcgaagtatt gtatggatgg atcgctgtcg tctggtatga
    10621 ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc atttttggta caagcatcgt
    10681 gtaaggggtg gtgagttgcg cattaaataa cgccatagaa aaaccagtca atgcttcaaa
    10741 ttcgatattt gctgttccac cgccatagtt ttgagaaagc atattgccac tatatgtttg
    10801 atcagctact tcataataat ctgccaatgg ctcaccagat acttctacgc cgtttaatcg
    10861 actaggatct gagaaacttt cactcatcac gaacacgatg ttcggcgact cttcctctac
    10921 agttttattt ttttcatcgg ctagcttctg gtatttttca gtgatttctt tgatcttttc
    10981 tttcgaatat ccttcaggtt catccattgg ttctaccttc agattgtaca aaaacccacc
    11041 gataaaacca gtgttatagt agttcatttt ctggctgtaa ggtatccata gtgccgtttt
    11101 attatatgct tttcgcaaca aattatcggg attgttgaaa ttactgatat accctagcag
    11161 tccaattgcc acaagcagtg tcgttaatcg gatcaattgg aatcgccttc cttttttcag
    11221 gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt cctgcggcag ctaagatcac
    11281 tgtaaacagc atagtgccgg tcatatcttt cagcaatccg atttcagtga tcatcttcaa
    11341 atcgtctgga taaatcggtt cttgccgata actcattttc atataatttg caaaacctaa
    11401 tacaccgatg gctacgacat acgtaaggga acctaaaggg acagatcctg ctagtgcaat
    11461 cagaaaaata agaataatca gcaagacgag acaggctaag aagaattttt ccgtatgcca
    11521 agaaaaagca aatttcaacg caaggtctac ggatagatca ttttgacacc actgcaaaaa
    11581 taaattacta aaaacaacaa gaaaaaagat actgatgatt gctgctgttc cttttaaaat
    11641 ctttttctgt ttggtcgaaa gtctgcttat atatcttttc aaatgcttgc tccttcattt
    11701 ctatcaataa aagaatgttg cttctcttat tttagttgta cgaaattcga aagtaaagaa
    11761 cggaaagaag aattaatgaa atccaaatat tctctttctt tatggccttt ctggtttctt
    11821 taataagcgg acctttcgta ctccagaaat tttttctagc tgttgtttca cttgattgag
    11881 ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag acatgctcat tcttatggtt
    11941 acgcgatata tctgcgatcg ctagctcgtt ctcattcagc agagaaaata tctctgctaa
    12001 tatattggtt ttcttttgat aaaatatagt gaatcgaaac ggcgcttgga aaaacattcg
    12061 tgcggatgga taattgaccg cttctctaac cgtaccgaac aaaagataat cttttaaggc
    12121 ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga gtcgatcccc tagatgaggc
    12181 aacataagta tacggtcatt ttctaagaac tcttcttctg gaaaatcagt caagtattta
    12241 gccagatgtt gtttttccaa tgcttgcata acagcatatt tatcaacaat ttcacttctt
    12301 cctacattga taagtacagc atcttttttc attttttcta tattcgcctg atcgatcaat
    12361 ccttttgtat cttctgtcaa cggcagcatg actacgatgt aatccgaggt agaaagaact
    12421 gtttctaaat ctgcttgccg aacataatcc aactgggcat cgcggataga ataacccaag
    12481 acatccatcc ctaagctata gcaacttagc gctacttttg tgcctatcgc tccaagacca
    12541 agaagaccga tggttttgcc ttgcaattct cgtcccacat aagcacttct tttgttttct
    12601 gcctgttcta aaatattagg acctgttagt gtttgtacca tacgacttgc ttcgattatc
    12661 ggacgggaag ataagagcaa acagcacaaa acaagctctt ttactgcatt agcatttaca
    12721 cctggtgtat tcatgacgat cgtcccgttt tcagaagctt tttcaacgtt gatcgtgttc
    12781 accccaacac cagctcttga aattgctagc aaatctggtg taagccattc atctttcact
    12841 ttcgtactac gaataactat tccttcggct ggcaaatcat ctattatttt gaaatattcc
    12901 ccttgtaact tttctatctc ttgttctgga atagcatcaa tcaaatgaac gtgatacata
    12961 aaaccccctc cttactaaca agtatctcga aatttctgca attagacaac tgtttaccat
    13021 ttgacttcct agaagcaaaa agagcagtta gagcagagta ggctgagtga taaaaataag
    13081 gctatgacaa gttactgcca taagccttat tttcgagagc aaaatattga gggtgagata
    13141 tcttgataaa tggtgttccg ttagctgaca ttgataataa gactaaattt caaaaaagtt
    13201 gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac taagtggctt tttcacaacc
    13261 tctttttatc taataaccac tgtttccaac tcaactaatt ttgcccacgt ttcaatttga
    13321 tctgttgtta gatttagcga gacaacggta tgatgaccgc cacctgcctg aatccatgaa
    13381 tggacacctt catggaaatt cggtttagtt ttccataaca cacgtgcaac aggtaaatta
    13441 ggtgcttcag tcgttggttc aaaggcttct acttcattga tcagcagttt ataatgcgta
    13501 ccgaaatctg ccatcgatac gactacccct tctcctgctt tcccatcaaa tacaagacgc
    13561 gccggatctt ctctatctcc cattgataaa ggagaaacaa cgattcttgg tttcgtcgca
    13621 gcaagcgtcg gatctacttc catcatatgg gattccaaga ttgcttcttg gccactagct
    13681 agctcgtaag tataatcttc catgaatcct gtatctttcc ctcgagccat gattttcagc
    13741 aaccgatcaa tcgctgcagt tttccagtcg ccttccccag caaaaccgta tccttctgcc
    13801 atcaaacgtt gtacagctag tccaggtagt tgtttcattc catacaggtc ttcaaaatta
    13861 gtcgtaaaag cgttatagcc tccttcttct aagaaacggc gaataccgat ttcctgctga
    13921 gcttgtactt ttacatgttc ctcccatgtc ttttcttcat aatcaccata gtcgaaggta
    13981 tacaattcct tgtactcttc aaataattga tcgacttctt cgtctgatac gcgatcgatt
    14041 acttgtacaa gatctccgat accaaaataa tcaacagtcc aaccaaattg aatctgtgct
    14101 tccactttgt ctccttctgt gacacccacg tttctcatgt tgtcaccaaa acgagccact
    14161 tttattccaa aactttcatt ataggctact gcaacatcca tccaatcagc aatctctttt
    14221 tggatctcag gtcgtttcca gtagccaacg acgattttat tttgtttatt taatcgagca
    14281 ttgatgaacc catattcgcg gtcaccatga gcggattgat ttaaattcat gaaatccata
    14341 tcgatcgttt tccaaggaat gctttcgtta tattgcgtag ctaaatgaag caatggtttt
    14401 tgtagaagct ttgtcccgcg gatccacatt tttgctggtg agaaagtatg catccaagtg
    14461 atcacacctg ctacttcttc tcgataattg acctctttca tgatttttgt gatcgtatca
    14521 gcagtaactg ctaattcttg aaggcggatt ggataaggca attggccatt ttcattcagt
    14581 tcatcgacca tttcttgtgc atgtttcttt acttctcgca gtgcttcttc cccatataaa
    14641 tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa tattcaacat aattagccac
    14701 tccttttatt tgttttgtcc gtaatacgca ttttttccgt gttttcgtaa ataatgttta
    14761 tctaaaatcc gttgaggcag ctcttctgaa aatgaattga gctgtctggt aaataaattc
    14821 atcttgcata cttcatctag tacgactgcg ttcattaccg cactttgcgc atctttcccc
    14881 caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa ttgctaaggg atcaattttg
    14941 cgttctttga atgtttctat gataactttt cctgtttcat attcgtaccc gctgtcgatt
    15001 tcctgttgtg tcaaaaatcg agcacaaggt accgatccat aaaaagtatc tgcatgcgtc
    15061 gtccccatcg caggaacatc caatcctgct tgtgcccaga tcgtcgccca tgtcgagtga
    15121 gtatggacga ttcctccgat ttgaggaaat gaacgatata aaacagcatg agtaggcgta
    15181 tccgaagagg gattgagttt accttcaatg atatgattgt ccaagtccac aacgaccatg
    15241 tcgtctgctg tcatctcttc ataactgact ccgctaggtt tgataacgaa atatcgtgtt
    15301 tcaggatcaa aagcgctcac atttccccat gtatatttga cgagtccgtg tttaggcaag
    15361 tcaagatttg cttggtatac ttcttctttc agttgttcca gcattttcta tcactccttt
    15421 ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc ccctcacggt aacgtctcat
    15481 aaatatctga tagcctgcta tttcttctgc tgaaggtgtg atggtgattc cttggtcttg
    15541 tccaaacacg tcttctgcca agaagttttc taaagacttc ccatgtcgat ccatcatata
    15601 ggctgctaat aacgcgattc cccacgctcc accttctccg gctgtgtcca tcactgtaac
    15661 tggtgcctcc atagcagatg ctagaatccg ctgtccaact tctggtgttt taaaaatccc
    15721 cccatgcccc actaaacgat caatttggac attctcagat ttcaatatat ccattccgat
    15781 tctcattgca ccaaaagcac tggctaaatg gatccgcatg aaattcgcta aatcaaaacg
    15841 gctacctggt gtacgtacaa ataatggcct tccttcattt actcctgtaa tgttttcacc
    15901 ggaaaaataa ccataagaaa gtaatccccc gcaatccgta tcgccttcta gcgctttgtt
    15961 gaataatacc gtgaataatt tatctctgct tatctccata cccaaagaat tggtaaattc
    16021 ttcaaaaata ctgacccaag cattgatatc cgaagagcag ttattcgtat gaaccatgcc
    16081 tactagttct cctgaaggtg tagtgaccat gtcgatttcc ggatggacat tttctagttc
    16141 tttttctaat acgatcatag cgaaggcgga agtaccagcc gaaatgttcc ctgtcccttt
    16201 ggctacactg ttcgtagcga ccataccggt tcctgcatcc ccttctggcg gacaaaccgg
    16261 gattcctggt ctcaaatttc ccgtagggtc gatcagataa gcccctgatt cgcttaactt
    16321 gccagcttct tctcctgcta ctagaacttt aggcaaaatg tgatccaagt caatcgctac
    16381 gtgcgctttt tcgattaact gaccaaaaat atttttcatt tcttgatggt aggttttcgt
    16441 tgaaggatcg ataggaaaca tgcctgatgc atctcctacg cctagtactt tctcaccagt
    16501 aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa ttaatttctt tcacatgttc
    16561 ttctttattc aaaatagatt gatacaaatg agcgatactc catctctgcg gaatattgta
    16621 ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg attgtatttc gccaagtacg
    16681 aaatggtact agtaactgtt ccttgctatc aaatgctaga taaccgtgca tcattgcact
    16741 gaacccaatc gacccgattg tcgtaagatt ctcgccatag atttcgaata cttctgctgc
    16801 catttttcgg taactgacgc gcagtccttt ccaaatatct tctaatgaat aggtccagat
    16861 accattttct agtttgtttt cccactcata actaccagcg gcaataggag taaaagatga
    16921 atcaatcaat acagctttga tcctagttga tccaaactca atccctaaag atgtacgacc
    16981 atctatgaca tctaacatac tttgcgcatt tacttccttc ataatgattt aactccctcc
    17041 ttctcgtcaa acctgatcaa accgtctttt tagtagcgct tacaatgatt attgtatact
    17101 tttgtacgta catgtcaagc agttatttac aagaatacaa taaaaaacac ttgttttgta
    17161 cgtacataag ttataaaatg aaattgctta caaaatctga tcaaccgcct tctcgaaatt
    17221 ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt tcctcatcgt ttatttattt
    17281 ttttggcgca tttggcggca ttctatttgg atatgatatt ggtgttatga ccggcgcctt
    17341 acctttttta cagcatgatt ggggtttggc aggtaaagct tctttgatag gatggatcac
    17401 gtcttcggtc atgttaggtg cgatccttgg cggctcttta tctggtcttc tttctgataa
    17461 gcttggacga cgaaaaatga ttcttctttc tgcacttatt tttatggctg gttctgtcct
    17521 ttctgcatct gcccctcaca atggttctta ttttctgatt gcagcaagga ttcttctagg
    17581 tttagctgtt ggcgctgcat ctgccctagt tcctgcttat atgtctgaaa tggctcctgc
    17641 tagactaagg ggaagattgt cagggatcaa tcaagtgatg attgcttctg gcatgctcct
    17701 ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa acgatggctt ggcgcgtgat
    17761 gttagggctc gctgctgtcc ccgcgctgat tttatttttc ggtgttttag ctctaccaga
    17821 gtcaccgcgt tttcttatgc aatctggccg cttggaagag gcaaaaagag tattgaacta
    17881 tattcggaca ccaaaagaag ctgaacagga attcgaacaa atccagctga atgtcaaaca
    17941 agaaaagaca accggaactt cttggcatac tttgttttta gaaaaatatc gttccttagt
    18001 cattgccggt attggtgtag cagtattcca gcaatttcaa ggagctaatg ctatttttta
    18061 ctatattccg ctgatcgtag aaaaagcgac tgggcatgca gcgagcgatg cattgatgtg
    18121 gccgatcatc caaggaatca ttttagttgc tggttctttg ctctttttgg ttattgcaga
    18181 caagttcaat cggcgaactt tattgaaaat aggcggtagc gtgatgggac tttctttcat
    18241 cctaccagct gttttaggaa ctgtactgga tgctcataca aattcgttgt tgattctctt
    18301 gtttttatgt atttatgtcg cattttattc ttgcacatgg gctccactga catgggtaat
    18361 cgtaggcgaa atattcccac tagctgtccg cgggcgagca tctggcctag cctcttcttt
    18421 taattggatt ggttctttcc tagttggctt gctttttcct gtgatgactg cttctatgtc
    18481 tcaagaaatc gttttcggca tttttgggat catctgcttt ttaggggttt tatttattca
    18541 agagatcgta ccagaaactc gtgggaaatc tttagaggaa atcgaacaaa gtgcatctaa
    18601 aaaaacttat cctaaacgta tttctatcca tcattcttaa agaggttgtg acagaagtgg
    18661 tcagcttcaa gaaataagaa ggaaccgctt agtttccagt cacaaggaac aatttcaaaa
    18721 aacagcttct catgtttgat gaatattgtg acttgtgacc agttggtagg agaacaccgt
    18781 ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg atgacaaaag ctctcacaaa
    18841 cattgcgacg agtaaccgta ggagcaataa gaggcggtaa aaacccgtct cttatttttc
    18901 gttttctttc actgtttccc gttcaactag tacaggttca tagagaatat ttggctgtgc
    18961 ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca tcttgtccca acttttcttt
    19021 tggatgcgtc aatgttgtca attttttatt ccctaaatgg cttaaagaag aatcgtctgt
    19081 tcctacgatc gataggtcat ctggaatatg atagccattt tctgtcaact tatctagcat
    19141 taaactagct atttgatcgt tatagcaaat gatgccatct aaagcttgtt cttctttcaa
    19201 ccgttgtact gttttttcag cgattgtttc tcgtgtttcc gtggtgaaag taatgatatc
    19261 ttccgtcaaa aaacgcacat tatactgttc acacgcgctg ataaaacctt tcatcctgta
    19321 ttttccttga agatcgtcga tttttgtgat gaacaacaga cgctgatgtc ctttttttat
    19381 caaatattct gctgccataa aaccgacttt cacatcatct aaacaaatat aagaggcatt
    19441 taactcttca taagcagcat tgatcatcac catcggaatg ccttgttctc tcaaagcaac
    19501 ataaagtgct aagtttgggt tatactgatt acttttcgta ggctcaacga tcaaaccatc
    19561 taccccataa gcaatcattt tcttcaaaca ctctttctcc aattcgtgat cgttattcgt
    19621 actggctaaa agcaatgaat acccagattc tcgtaattca ttttcgattc ctcggataat
    19681 cgacggaaaa atataatcag agagatatgt ggtgatgaca ccgatcgttt ttgttctgtt
    19741 caatggtgtt ttttctttct tatagctgtc atctacatat gtgcccgacc ctttttcttt
    19801 acgcagatag ccctcattta ccaacacagc gattgcttgc cgaatcgtat gtctgctgac
    19861 gtcatatatt tcttgcagct tgaattctga gggaatggtt gtatttactt cgtattcctc
    19921 acgaaggatc ttttgtttta tctcttctgc aatatcttga tattttgcca tttgccttgc
    19981 ccctttttat ttgtacgtac atatatcata acgagtctgc aaggtttatt caagcaaaaa
    20041 tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc accaaattct ggaaatcctt
    20101 gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt aggattgtct aacggatccc
    20161 ctttttggtt cttctctggt cgagcatgat agatcaaaat atcaaagcac ccgtcttctg
    20221 aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt tttttcagaa gaaacgaata
    20281 ctggctccga taatttatgc catgattctt ttaataaaag attcttgtgc tcatcagccc
    20341 acagcaggcc catggcatag ttttcatctg ttgcactacc agaatacgta atgaatattt
    20401 tcccattgcg agtaaggact gctggacctt catttactga aaaaccccgt ttttcccatt
    20461 catactcagg tatagaaagg aggatctgct tcccctttaa cgtccaagga ttatccattt
    20521 cggatagata aagattagaa ttcccagtaa tttcaggatc ttgttgggcc cacacataat
    20581 atagctttcc ttgagaagag aaagttgttc cgtctaaact gaaagtttcc cttttcgttt
    20641 ttatttgtcc cttttctatc caatctgtat tcattggatc tatatgctga ttttcaagta
    20701 cgaacatgcg gtgatgatga gattcatcac gtatagtttt atgattgctt gcagcaaagt
    20761 aaatatacca ttttccattg atataatgga cttcaggtgc ccagatcaat tcactcatcg
    20821 gtccttcttc atgagcctgc catacaatca ggcttgcagc atgttctaat tcattcagtg
    20881 actttgccct tcttaattca atcacttggt agcctggaac cgatcctgta aaataataat
    20941 aaccatctgt atgtttatat acccaaggat ctgcccgttg ccgaacgata ggattattat
    21001 aaagtgtctt catcataaca aatttcctca cattttcttt attctaatta cattccaaga
    21061 gtatttttct atccctacat ttaattcgtc atgtttttct attgaaaaat agtttatttc
    21121 aggttttatt ttttctggtt cttcacgtgt atttttgtcc aacagatcgc tactggttaa
    21181 tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt tcaatcgtta tcttttgttg
    21241 ttcatttgtg cgattgatca agaaaatagt cagctgattg ccgttatcta cgatcacact
    21301 gtccacataa ggaacatttt ttatcttttc acaatcataa acagggcaaa tgatttgttg
    21361 tttcaatact tgacctttag catatttcga taagtgttgc atcacataat aagttggatt
    21421 cctccatgct tgtcctttag catctgtcag tattaaaggt attgtgttta ctaacaatga
    21481 ttgacaacta atctttacgc gatctgcgtg tcgtaaaata gccagcccta atgaaccaaa
    21541 taataatgta tcttccatag taaagtaaca ccaatcgatg ggactaccga cttcaaaatc
    21601 cctatgtctt ttttccggtt gggcatgtac attccattca tcaaacgcta gatacattgt
    21661 tttctcactt ctcaaatatc cttttacata atcacaagta gcaacgattt cttcaatttg
    21721 acgatcgaat cgataagctt ttgcaaggta tgtcgctgta tcatcacgct catttttagg
    21781 aggtttagtc aagtcttctt catcatactt atcaatatag ttatgcaaag ctaaataatc
    21841 tacattctca taagcttctt ctaatgcgat acgatcccat tcagggtacg atggtaaacg
    21901 tggcgtagag cttccaacta agattgtttc aattgaatca tccactaact tcatgacttt
    21961 tgaggcttca tttgctaacc taccatattc ataagcactc ttagccccca cttgccatgg
    22021 accgtctagc tcattcccta aacaccataa tttaacgcca aaaggctttt tttgcccatt
    22081 ttttattctt aaatcactcc agaaggttcc ttttggaaaa ttacaatatt ctactaaatt
    22141 ttttgcttca tcaattcctt ttgttcctaa attcaccgtg aatatcggtt tcgcttgtac
    22201 atgactgatc catttaaaaa attcgtggag tccaaaagaa tttggttcta tacctcgcca
    22261 tgctaaatca attttttttg gtcgttgttc tttcctacct acagtatcca cccaatcata
    22321 accagatgta aagtttcctc caggatagcg aatgacacct aattctaatt cctttacata
    22381 agagaggaca tcttgtctaa acccgtcacc atcagctgtt tcatggcccg gttcataaat
    22441 ccctgtatag acgacacttc ccatatgctc aacaaatgag ccaaacagcc gagaactgat
    22501 ctctcctacc gtaaaatcct ctctgatttt tactttcact tcttcgtcca acagctttcc
    22561 ctacctttca tttcctatag tcaagataat agatacaagt ttccatacgc caccagcaaa
    22621 gataaaaata aaaggaggga aaagaaatat gagcagtaca aatcctaata acaatacgca
    22681 acaaagaata tttctcaaaa aatcaaaaaa tactttccat gcactttgtt caaaaatttg
    22741 taaaaatgtg atatctttgt tttcagaaat cacatatatc caggataaaa gaaacatgga
    22801 ataaataagt aaaactacat atccactaat tgaaagtacg attgtctgtt cttctaataa
    22861 ttgattaaca agaaaataaa taatgatcgt taacagacta aggaacaagt tcttaccaaa
    22921 atgttgcttt attccttgaa agaaaaaata aatgatattt cctgatccct cacctttcca
    22981 ttgcgccatt acataaaaaa cagcacatgt agcaggcacg atagtaataa caggcaaaca
    23041 cacgattacc cagagaatac ctaataagat gtaatcagaa accacaatca actgtccata
    23101 aagtttagtc tcaactattt tcatcttcct cactctttca ttccagacat agctgttcct
    23161 tgaacaaaat atttctgtgc aaaaatataa accactagta atggcagcat tgaaataacc
    23221 gttccagcca ttacaggtcc ataaaaagaa atgttctgcc ctgtaaataa agctaaccct
    23281 gctggtaaag ttctcatttt tgaactactt gtcaatatca atggataaag caagtcattc
    23341 caactattca tcagcgtgaa aatccctaat gataaaagag taggtttgct caatggcaac
    23401 atgattttca aaaaaatatt aaactcactt aatccatcaa tgcgtgctgc ctcttccaaa
    23461 gaatctggta aagtaataaa aaaggaacgc atcatgaaga taccaaatgc agttgtcata
    23521 cgaggaatga tcaaacctgc ataagtatca agcatgccaa gcaaattgac ttcaataaat
    23581 agaggaatca tgaagacttg gaatgggatc atcattgtca gcaaaacgaa atagaacaaa
    23641 actgattttc ctttgaaacg catacgagca aatgcatacc ccgcaagaga atcaaagaat
    23701 acagaagtga tcactgtccc tccagcaaaa atcactgtat ttttgatgta atctaataaa
    23761 gggatcgttg tccaaacctt aatatagtta tctagggtat agctatctgc aaatattcta
    23821 ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag aaataatcag ccacaaaaaa
    23881 ggaaacgcaa taaccaacaa tataactagc atactaatcg ttcgaatgat ttttttacca
    23941 ctaactgctt ttcgactatt cacagtaact gtaatcgagc tttctggtgc catacaggtt
    24001 tccctcctat ttcatttgtt tttctttccg tgacatgtac acattagtgg agatagctat
    24061 agttgcaaca atgataaaca aaaccgaaga cagtgctgat gcatagccca attcataagg
    24121 tgctgtaaat cctcgactgt aaatatattg aacagctgtt tctgtcttaa attgtgggcc
    24181 accagcggtt gcaacatata cttggtcaaa cacttgcatc gaaccaatca gattcgttaa
    24241 aatacaaaaa cctaaggagg gaacgatctg aggtagcgta atgttaaaaa actgctgtcg
    24301 atttgttgca ccatccattt gagcagattc atacaaagag ggagagattc cctgaatagc
    24361 agtcaagaga atcgtcatag taacaccaaa attcttccaa acagtcatga tggctaccgt
    24421 cggcatagca agggttggat cgcgaaaaaa ttgagggatc ggcaagccga cttgtgaaaa
    24481 caagtaagga atcgccccta cattcggatc aagcaacata gaaaaaataa tcgcaattga
    24541 cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga caaaaacgat tgaatctggt
    24601 atttttagat aatgctactg ctgctaataa accaacgata atttgtgttg gcgtttcgag
    24661 tacggtgaaa tataaagaat gaatcaacga attaactgct cgttcatcac taaatatacg
    24721 tgtgaaattt tcaaaactta caaaatttct tgatgtaaag aaaatattca aatctgtaaa
    24781 actaatagca aatgttccta aaagcggtat gacagtaaat aataataaaa ctaaaaaaga
    24841 tggagttaaa aacaaatacg cagcacgatc aggacgattc caatatttgg tgaatccgtt
    24901 catatagtcc acctcatctt tcattttgat aaaatataca atcttggttt aaacgaatct
    24961 gaataagata tccgaacaat cgctgtgatt tttgcaataa cacctatctg caaatactag
    25021 gtcgatcatt cagatatctg tatcttgcca acagtctatc ctgtataaag ttctgtacta
    25081 tttttgcagt aattgattga tggcatcatc tgcatcgttc atgagtgtct caggatcttc
    25141 tcctgctaat aatttttcaa tcagtggatt gatgatatcg ttattgatcg cagagatttg
    25201 ggtaaaacct ggtaaatatg gctctgcata gtccatttgt ttcgataatt cagatacaat
    25261 aggatcattt tttacttctt catcttttgc tactgaatcc aagtaggcag gaaaaccatt
    25321 ttcgatactc cattttttac caatttcagt tgtattccaa tatttcaaaa actcatagac
    25381 agcttctttt ttattattat ctgtacttgc cggaatggca tatcctacgc catctaaaat
    25441 agctgttttt ttgtctgaat cactcccttg tggtaaggta gtcacattat aatcaatctc
    25501 gtttgctttc aatccgttat ttaaccatgg cccattgatt tcaatggcta gttgaccagc
    25561 attcattaaa ttgtccattt cggcgccaga aatcgattct ggaccagtct tgtctacatg
    25621 gatcattttt tggatcgctt ttaacgtttt aaggttctca ggagacgcaa attcagactt
    25681 agtataatca tcgttcacta atttaccgcc atttgctaac atccagttat ataacggtgc
    25741 agttccatcc ttattgaaga caaaacctga tacattttta ctactatcag ttaattttgg
    25801 tgccatttct gtcaattctt cccaagtagt aggtggttgt tcagtatcta atccagcttg
    25861 tttaaacaaa tcattattcc aatacatata catgccttgt acctgcatgg ggacaaaata
    25921 ctgtttacct tctacttcac ctaattcaat agccgtttct ttaaaatcag atttatctac
    25981 tccatcatat tcccagaaat cgtctaatgg ttgtaccgct ccgttttcta catactgtgc
    26041 aaaatctgga taatttaatg caataaagtc aggtgctttt ttagaagaaa tcgctgtagg
    26101 aagtttttca ttcaaattgg cccaagtcat gatatccatt tcaattttga tgtttttatc
    26161 atttgtttta ttaaattcat taacgatttc ttgcaatatt tcaccatcag atgcagtaaa
    26221 cccattccaa aaagtcaaag tcacttcttt atcagaatca gattctgctg aacttccgcc
    26281 accgcaagct cctaaaatac ctgttgcagc taccaacaaa gagccaatta accatttttt
    26341 acctttcatt ttttgttcct ccttcatctg ataaacgaca tctgaccaca ttccaagata
    26401 gcggtttcaa taaacaattg attttcccat cttcagcttt tacatttgtt gaatgacttg
    26461 gttttacttg ttcactttta ggaagattag ttgcttttgt atcaaatcca ctcatttcag
    26521 aaaaatcaat cactccctct aatgataatt cagacaagtg aacagttaga ttcatttctt
    26581 cttctccacg gttaactgca aatatgacaa gttctttgtc atttattacg gctatacttt
    26641 ctaaatacgg cacttcttca aaatcttccg tcgcatagtt tgctgaacga atttgtgggc
    26701 ttaacacctc tccttgtcca tacacagcag cttgcataaa tggataatag atcgtctgtc
    26761 tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat aacatttacc aattgagcaa
    26821 gacaagcaat ctttactcta tctgcatgtt tcaataaagt gattaataga cagcctaata
    26881 gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg aggagcaatc tgccaaggcg
    26941 ctgcttgctc atcttgttcc gctgaatgat accaaatatt ccattcatca aaggacaggt
    27001 tgatctgctt cttactatgt ttcttcgcct tgactgtatc gcacatcgct gtcacttcag
    27061 aaatgaaccg atccatgtct aaggaacgtg caagataatg agaaagatca tttttttggt
    27121 tgccataata ttgatgcaga gacaaataat ctatttgatc gtacgcttga tcaagaactg
    27181 ttaactccca atcaccaaat gtaggcattt gccgattcga acttccgcat aaaaccacct
    27241 caattgaatc atctacgagt ttcatcgcct tagccgtttc ctctgctaac cgtccatatt
    27301 cttccgctgt tttatgtcca atctgccaag gaccgtccat ttcattccct aaacaccatg
    27361 tcttaattcc aaaagggctc tcttgaccat tcgaacgcct taaatcactc caataagttc
    27421 ctttaggaaa attacaatat tcgactaaat tacgagctgc atctactcca cgagtaccca
    27481 gattgacagc catattgatt tcggccccca catgctgaca ccagttataa aattcatgaa
    27541 tcccaacttc attcgtttca attgtccgcc aagctaaatc caaccttcta ggtctatttg
    27601 ctatgggccc aatactgtct tcccaacgaa atcctgacac aaaatttcca ccaggatatc
    27661 taatcatggg aatgttcaat tcttttacca agtgaatgac gtcttggcga aatccacttt
    27721 tattagctga tggatgatct ggctgataaa tcccttcata tactgctcgt cccatatgtt
    27781 caataaatga actgtacaac cgcggatcaa ctttgcctac acgcttgaca ctactaatca
    27841 acattgtggc ttccataact gacctccttt aatttataca tgaataatac acctcgttga
    27901 aagcgttgtc aataatataa atgttaaact tacttatttt ttatcgattt taattaacca
    27961 aaatattaat aatgattaaa acaaat
//
LOCUS       DAZKNO010000015        27759 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000015 DAZKNO010000000
VERSION     DAZKNO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.015"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..1370
                     /gene="lysA"
                     /locus_tag="ACG8MY_000488"
     CDS             111..1370
                     /gene="lysA"
                     /locus_tag="ACG8MY_000488"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0429718.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1357..2157
                     /gene="dapF"
                     /locus_tag="ACG8MY_000489"
     CDS             1357..2157
                     /gene="dapF"
                     /locus_tag="ACG8MY_000489"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0429719.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2160..3041
                     /gene="dapA"
                     /locus_tag="ACG8MY_000490"
     CDS             2160..3041
                     /gene="dapA"
                     /locus_tag="ACG8MY_000490"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0429720.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3038..3754
                     /gene="dapB"
                     /locus_tag="ACG8MY_000491"
     CDS             3038..3754
                     /gene="dapB"
                     /locus_tag="ACG8MY_000491"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0429721.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3760..4455
                     /gene="dapD"
                     /locus_tag="ACG8MY_000492"
     CDS             3760..4455
                     /gene="dapD"
                     /locus_tag="ACG8MY_000492"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0429722.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4471..5472
                     /locus_tag="ACG8MY_000493"
     CDS             4471..5472
                     /locus_tag="ACG8MY_000493"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0429723.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5469..6830
                     /locus_tag="ACG8MY_000494"
     CDS             5469..6830
                     /locus_tag="ACG8MY_000494"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0429724.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6790..7941
                     /locus_tag="ACG8MY_000495"
     CDS             6790..7941
                     /locus_tag="ACG8MY_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0429725.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7916..9085
                     /locus_tag="ACG8MY_000496"
     CDS             7916..9085
                     /locus_tag="ACG8MY_000496"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0429726.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9260..10639
                     /locus_tag="ACG8MY_000497"
     CDS             9260..10639
                     /locus_tag="ACG8MY_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0429727.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10920..11717
                     /locus_tag="ACG8MY_000498"
                     /pseudo
     CDS             10920..11717
                     /locus_tag="ACG8MY_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11906..12574
                     /locus_tag="ACG8MY_000499"
     CDS             11906..12574
                     /locus_tag="ACG8MY_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0429728.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12774..13160)
                     /locus_tag="ACG8MY_000500"
     CDS             complement(12774..13160)
                     /locus_tag="ACG8MY_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429729.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13132..14514)
                     /locus_tag="ACG8MY_000501"
     CDS             complement(13132..14514)
                     /locus_tag="ACG8MY_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0429730.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14670..15857)
                     /locus_tag="ACG8MY_000502"
     CDS             complement(14670..15857)
                     /locus_tag="ACG8MY_000502"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0429731.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15864..16895)
                     /locus_tag="ACG8MY_000503"
     CDS             complement(15864..16895)
                     /locus_tag="ACG8MY_000503"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0429732.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(16911..18365)
                     /locus_tag="ACG8MY_000504"
     CDS             complement(16911..18365)
                     /locus_tag="ACG8MY_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0429733.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            18540..19445
                     /locus_tag="ACG8MY_000505"
     CDS             18540..19445
                     /locus_tag="ACG8MY_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0429734.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19536..20213
                     /locus_tag="ACG8MY_000506"
     CDS             19536..20213
                     /locus_tag="ACG8MY_000506"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0429735.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20210..20380
                     /locus_tag="ACG8MY_000507"
     CDS             20210..20380
                     /locus_tag="ACG8MY_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429736.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            20494..21390
                     /locus_tag="ACG8MY_000508"
     CDS             20494..21390
                     /locus_tag="ACG8MY_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0429737.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21445..22815)
                     /locus_tag="ACG8MY_000509"
     CDS             complement(21445..22815)
                     /locus_tag="ACG8MY_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0429738.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23053..23799
                     /locus_tag="ACG8MY_000510"
     CDS             23053..23799
                     /locus_tag="ACG8MY_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429739.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23796..24635
                     /locus_tag="ACG8MY_000511"
     CDS             23796..24635
                     /locus_tag="ACG8MY_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0429740.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24636..25586
                     /locus_tag="ACG8MY_000512"
     CDS             24636..25586
                     /locus_tag="ACG8MY_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0429741.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25727..26479
                     /locus_tag="ACG8MY_000513"
     CDS             25727..26479
                     /locus_tag="ACG8MY_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429742.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26498..27649
                     /gene="queG"
                     /locus_tag="ACG8MY_000514"
     CDS             26498..27649
                     /gene="queG"
                     /locus_tag="ACG8MY_000514"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0429743.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9117 a         5043 c         5830 g         7769 t
ORIGIN      
        1 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
       61 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
      121 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
      181 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
      241 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
      301 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
      361 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
      421 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
      481 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
      541 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
      601 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
      661 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
      721 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
      781 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
      841 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
      901 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
      961 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     1021 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     1081 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     1141 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     1201 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     1261 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     1321 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     1381 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     1441 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     1501 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     1561 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     1621 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     1681 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     1741 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     1801 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     1861 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     1921 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     1981 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     2041 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     2101 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     2161 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     2221 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     2281 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     2341 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     2401 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     2461 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     2521 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     2581 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     2641 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     2701 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     2761 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     2821 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     2881 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     2941 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     3001 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     3061 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     3121 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     3181 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     3241 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     3301 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     3361 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     3421 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     3481 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     3541 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     3601 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     3661 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     3721 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     3781 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     3841 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     3901 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     3961 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     4021 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     4081 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     4141 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     4201 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     4261 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     4321 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     4381 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     4441 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     4501 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     4561 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     4621 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     4681 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     4741 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     4801 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     4861 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     4921 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     4981 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     5041 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     5101 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     5161 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     5221 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     5281 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     5341 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     5401 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     5461 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     5521 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     5581 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     5641 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     5701 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     5761 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     5821 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     5881 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     5941 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     6001 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     6061 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     6121 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     6181 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     6241 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     6301 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     6361 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     6421 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     6481 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     6541 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     6601 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     6661 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     6721 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     6781 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     6841 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     6901 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     6961 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     7021 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     7081 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     7141 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     7201 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     7261 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     7321 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     7381 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     7441 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     7501 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     7561 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     7621 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     7681 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
     7741 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
     7801 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
     7861 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
     7921 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
     7981 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
     8041 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
     8101 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
     8161 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
     8221 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
     8281 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
     8341 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
     8401 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
     8461 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
     8521 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
     8581 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
     8641 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
     8701 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
     8761 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagatta
     8821 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
     8881 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
     8941 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
     9001 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
     9061 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
     9121 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
     9181 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
     9241 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
     9301 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
     9361 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
     9421 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
     9481 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
     9541 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
     9601 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
     9661 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
     9721 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
     9781 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
     9841 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
     9901 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
     9961 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    10021 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    10081 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    10141 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    10201 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    10261 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    10321 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    10381 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    10441 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    10501 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    10561 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    10621 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    10681 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    10741 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    10801 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    10861 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    10921 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    10981 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    11041 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    11101 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagtt ggagcagtag
    11161 ccaaaaatcg gatccatcgt cgtttctaca ccagtatctg ccatcagaaa atcacgacgg
    11221 atacaactga atttaaatac catgaagtgg atgccaaagg cattatccgt caaaagaaat
    11281 tgtatctaga tccttttatg gatatgttca atagtgagat tttatcctat cgtatctctg
    11341 aaaagcccaa tgcattagcg gtaatggaag gtttggagga agctatccag accactaacg
    11401 attgtcccta tcgtcgtacg tttcactcag atcaaggttg ggcgtatcaa atgaaagctt
    11461 atagaaacaa attgaaggag tacaaaatct ttcaaagcat gtctcggaaa gggaactgct
    11521 tagataattc acttatggag aatttttttg gcttattaaa acaagagatt tttcacggga
    11581 aagtttataa ctgtttcgag gaattaaaat cagccatcga tagctatatc tattactaca
    11641 ataatgaacg gataaaacag aaattaaact ggcaaagtct cgtccaattt cgtaaaacaa
    11701 ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa aaaaggagtg gatttctcca
    11761 ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca cagtcctttt tttagtaaaa
    11821 ggatacattt cttccttgta tattccatgc gtgttcgtga gaaagcatat ataatttaat
    11881 taacagatag ttagggggag aaaaaatgat tttttcaatt acaatgaaac tggtgttcgg
    11941 gttgatcggt ttattattag tggtacgttt gcttgggaag aaatccatgt cagaaatcac
    12001 accgtttgat ttagtctata cgcttgtttt aggtgggatc ttagaagaat caacctatga
    12061 tgacaatgtc catgtagggc atgtcctatt tgccattgcg ctttgggcag taatgattta
    12121 tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat cgttgggtca aaggcgagcc
    12181 atctgttcta atcaaagacg gcgtcatcaa catgacagag ttgacaaata accatatcga
    12241 gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc ttttttcttg aaaatgccaa
    12301 acatgttatc ctagagaatg ccggtcaaat gagtgtattg aaaaaatcag atgaagacaa
    12361 agctttatcg atcctgcttg tagatgaagg gcaaattcag cataaagtgc ttcagtccaa
    12421 tcaattgact gaagcatggc ttatggagaa cttgaagaaa gaaggctatg cagatgtaaa
    12481 acagctgatt tatgttgaat ggtcagaaga aaaagggctc tacatactaa ataaagaaga
    12541 cgtcatgcat gaatcttacc gaatagatgg atagatagac agaggttgtg aaatcagcgc
    12601 cttggcttgt tactcaaaac taagcgactt tttcacaacc tctgatatct aatatacgtt
    12661 gttccagaag ttgcttcttc gaaaaataag ccaaaattcc acaaaaattt aagaagcaat
    12721 tttcgaaaaa tcctttctta ttttttgaag ttgatcactt ctgtcacaac ctctcatgtc
    12781 acaaaccatt tttttgtttc ctttaactca atataccgta cgtcttccca caatatcggc
    12841 tgatggtcaa tcataaagta atcttcatag gcttcaccaa tgaagtgccc ttcgacctca
    12901 tccaagagtc ttccaaattc atcttttaaa ttgagctgaa tgattactgc tttacgagat
    12961 aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg tcatttgttg tttaggaaga
    13021 ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt tgactagttc ttccatcgca
    13081 aaagcagtcc cccattttaa tccaaatggc cgatctcgat actcgttata gtcaatgaat
    13141 ttttttgctg tccgtctcat cttttccacc ttctaatcca ctcataccac ctgcatgccc
    13201 gccaacgaga gaactacgtg cgatggcgcg tcctccttgc aagagactat gcgcatggat
    13261 gattgattga aagccgtatt tttttctaat cgtatcgaca acaaaatcaa ctttcgcttc
    13321 atttatctgc ttttcaggat cttcaaaaag gttaagctgt aaacctgtcg cgtatacgag
    13381 gtcggaacag ttcacgccta cacgccgaat atcctggtag tggtaatgct ggtggaaaat
    13441 ggtcaataaa gcatctgcta gatctctgct agcatttgtc gcttcgattt tcatttgctg
    13501 gtgaaacccg gtcttgccat ttggatcaag atagcctaaa gagtatccta cccataagct
    13561 gactacttgt gtttttgctg cagatcgtct caagcgagtg ccgacttggt cggccatttc
    13621 tttgataacg atctcgattt cttctcgtct tgtataatct ctatctagga tttgactatt
    13681 tccgatactt tttgattttg gtgtatattc ttgtcccaaa aaacttcgat cgatgcccca
    13741 agcatgggca taaagctgag taccgatgac ccctagcttg cttttcaagt gataataatc
    13801 cgcgtgagct aaatcataaa cagaatgtat acccatttgt ttcagacgtt ttgctgttcg
    13861 tttgccgatt ccccaaaagt cagtgatatc tgagattttc cataactttt ctggtacatc
    13921 ttcataacgc cattcagcaa acatttgctt tccgtctttt gagccattgt ctaaggctag
    13981 tttagcaagt agcgggttat cgccaattcc tatagtcgtg tagattccgg tctgctcaaa
    14041 cacatcttta tggatcagtt tagccatgtc gtacgcattt gttttaccaa agagtgccat
    14101 gctatttgtt acatcaagga agctttcatc gacggaatag acatgatggt tcgcttcgtc
    14161 tgcatatttt ttatagatag aattgatttc tctgttcttt tccatataat aggccatcct
    14221 tggggcaaca atatataaat cacttggata ggggaaggga agatctctgg cacggctgat
    14281 attggtgatg ccgtaagctt tttttgctgc aggactggaa gccaagatta atccgctacc
    14341 ccgtgtttct gtcgaatcgg acggatagga catgactact aatttagttg taaggggatc
    14401 aagatccttt tcaacgcact ctacggaggc gtaaaaagat ttgcagtcaa tacataaaat
    14461 atctcttgaa ggttcttttt cataaacaaa taccggattt tgattcgccc acataacaat
    14521 cacctcttca acacaattag aacacacgtt ctctttccgt gtcaataaaa aacagcaatt
    14581 ttcaaatacg agcaaaaggc tcacatttgg aaattgctga gaggttattt ttatctattt
    14641 gtttttagct gagatcgttg atttttttat caaacatgaa cagcttctgg atcgatcttt
    14701 gttgtcccat ttccaatgtg tgcaacataa aattccggag catagccgac aactttcatg
    14761 tattcatcat agacattgtt tttgaaggca ggtatatttt cttctttgac aagagcaatc
    14821 gcacagccac caaatccagc tcctgtcatt cgtgcaccta agacaccttc ttgtttttga
    14881 gcagcatcta ctagtgtatc taattcgatc ccagttactt cataatcata gcgaagagag
    14941 tggtgagaat catttaacag ttgaccaaat gcttctaagt tcccagcttc taattcagct
    15001 tttgccttca aagtccgctg attttcagaa acggcatggc gagctctgcg tatcaatgta
    15061 tcatcgccga tttggtcaat agttgattca aattcttctt catctaagtc tcctaaagaa
    15121 gagataggca aagttttttg caaacgtgcc aaggcttctt cgcattcgct acggcgttca
    15181 ttgtattttg aatctgccaa ttcccgccgc ttattcgtat tcatgatgac gattgcataa
    15241 ccatctagtt tcactggaac catttcgtat ttcagcgtat ttgtatctaa taagattgca
    15301 tgatctagct caccgaagcc gatggcaaac tgatccatga tacctgaatt gacaccgatg
    15361 aattcatttt ctactttttt acctgtttgg actagttcca agcgatctgt ttttaaatca
    15421 aacagatctt ctaatacaac acctactaat aattctaatg aagcgctact ggaaagtcct
    15481 gcaccattag ggattgttcc ttccactaat aactcaaacc catgatcgat tgagtgtcca
    15541 gcttctttta atttaaggac cattcctttt acgtaatttg cccagtttgc ttttttatca
    15601 tattctagct catctaaagt aaaactaatg acaccgatat cgtcaaagtt tgtggaataa
    15661 actaatattt tattatcgtc tcgaggtgct gctacacctt gcgttccata agtgatggaa
    15721 gcggggaaga catggcctcc attgtaatct gtgtgttcac caatcaaatt gatgcgtcct
    15781 ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt cggcaaattt tgtgtttaag
    15841 cttcttttga tttccatgat gagttattcc tttcttgttt tgattttgaa ttctgtcact
    15901 gtttcaaata ctgatccggc ttttaaatga atcgaaccaa atgctgagaa gcgctctgca
    15961 cctggagctg tttgagtctc aaatgtgatg ccaccgtgat gtgccagttt tcggttgcgc
    16021 atttctggtg tttctgtgcc gaaatttgca gtgaaaataa ccacactaga agcatctgta
    16081 gcaatatcta cttggattct tttatcgggg ctagtcaagc gagcggcttc tttacccaag
    16141 ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa caagattttt ttgatccaaa
    16201 tcactagcaa aaacggacgc taatttttta ggtatttgaa aatcgaaagc acttcctgca
    16261 gcattttctt ttacccctat aggaatacta tctgttcgca aaggagcata agcttcgcta
    16321 ttgagccaaa gctcatgttg atcgattgac tgagaagcat ctcccgtcaa gttgaagtaa
    16381 acatgatttg tcggattgaa taaagtatct tggtcactgg tgccgcgaga ggtcactcgc
    16441 cagatattat cttttgtcaa agtatagcgg atttccacat ccattgtacc tggaaaacca
    16501 tgttctccgt cgggacttgt agtagtaaaa atgacagaag cttcattttc gccattcaaa
    16561 atgacataag accatttttt taattcaaat ccaggagcac caccatgcag attatgtcca
    16621 gtttttggat ctgtggctaa ttggtaagtt tttccattga gagaaaagtg gccattttcg
    16681 attctccctg cggtgcgacc gatagaagca ccgatatacg gatctttttc gatatattct
    16741 tcagcggtgt caaatcccaa aactaattct cgatcatttg attttaaact gacgattcta
    16801 gcacctaaat cagagataga aagtgctagc ttgtttttgt tctccaaggt tatcaagtga
    16861 taaccttggc caaaatcttt ttctgtaatc ttcattttca agatcctttc ttaatgggaa
    16921 ggaactactg catcgtgaag ttcatcttcc attgtatcag ctggagccaa tttgttttcc
    16981 aatttctcaa cgatttgtgc atgttttttc tctgtcaatg taatcttcca agcaaaaata
    17041 atagcggaga aaatcatcaa agcagcagga gcatagaatg cataagcttt gaaagtcgtt
    17101 acgccatgag cagtgatatc agcagcagta gcattaccag tcatcccaga agcgatagcg
    17161 acaaacccta cgatcccgtt agacaaagca ccagcgattt tatctagtaa agggcgaatt
    17221 gatagagtaa cagcttcgtt acggatacca tttttccatt gaccatattc aacagagtct
    17281 gtgatcgtca tcaaagcgga taagaaaatc aattgttgag ggaagtagaa caatactagc
    17341 ccgatgataa cgattggcaa acttgaacct gcgatcgtaa agaaaaagta agaagaaatc
    17401 atcattgcga tccctccaag gtaaacgtag cgacgagaga tgacgcgtgt aagcattggg
    17461 aatagtggaa cagctactaa accaagtaag gcagcaacta gaccaacgat ccaaaattca
    17521 gttggttggc caagtacata tttgaagaag tagaataata cggcagttgt tgctacattg
    17581 gcaatcgcat aagcgatata agcaagggca agccacatca actgatcatt ttgagcaact
    17641 gctttgaaca catctttgat cccagctttt tcattttgct gacggatcgc agtatcgact
    17701 tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg aaataacagc agcaatcaca
    17761 ccgaaccaga accagcctga agcgccttca atatgtttat gagtaacaag ataggtaaag
    17821 aaagtaacga tagggacaac gactagagta gtaccatttg cacctagaga agagccgaaa
    17881 cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg tactcaaagc tggaatcatt
    17941 gaccaaaaag cgatatcctt aaatgaataa aaactatcca ataagatgaa aacgattgca
    18001 aataatatga taaataaagt cgtattatga gtagctaagc caaagaaatt ggtaaagatt
    18061 gctaccaaga gtacggaact aacaacgccg ccgataacta accatggttt gaatttaccg
    18121 tattttgtac gagtattatc gatgatgcca ccaatcaaag gatcaaaaat aatttcaact
    18181 aagcggatac ctacaactaa gcttgtaact gtagcaatca tttttgcatt cgttgcctta
    18241 tctgttcctg cgaacatctg gcttgtcacg aataccataa aataagtgct taatgtaaca
    18301 tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca cacgttggat cattttgctt
    18361 ttcataatct gtattcctct cctgttcaat atataatact aagtaaaccg gttacaacga
    18421 ataacaaaga tcttttaatg acttaaactt aacaagaaat cgcttccttt ttaatatatt
    18481 tctgtgactt ttatttaact ttttgttgca ttatactaaa ggagtaagga gggatagacg
    18541 tggagcaatc gattttttta aaaaaggaac atcaacaagt agaaaaagaa ttgtattttg
    18601 atttttgtgg tttctctaaa acgatgccgt ttcattcttt tggtcctgct attcgcaatg
    18661 attacatcct gcatgtcgtg atggaaggca aaggatctta tcatgtgaaa aatcaacaat
    18721 tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg agactcgacg ttttacttat
    18781 cagatggaga agatccgtgg atgtattgct ggctttcatt tggtgggagt gctgcagaag
    18841 aaatcattcg gcagtcgctt ttcagagaag accaatacac gatggtctct tctgacatac
    18901 aaaaatatat cgacattatt ttagaatgca tgaaagatac ggaagaaacc ttggaaaatg
    18961 aattacagtt aacagcatta acctaccgtt ttttatcagt ccttttaaaa gatggcggaa
    19021 aggtcagcgg tggtgtacaa aaggttcatt cctctttagc tgttgaaaca gtcaaataca
    19081 tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat cgcccagcat ttagcggtca
    19141 atcggagcca tttgtcgcga gtattcaaaa atcatatggg taccagtatc aaggaatatt
    19201 tgatcggggt acggatcaat cgaggggcgt ttcttctttc tctgacggat gaatccgtgg
    19261 aaagtattgc ctatcaagta gggttcaata gtctagtcgt atttagccga atgttcaaaa
    19321 agagtaccgg agaaacggct acgaactatc gtaaacgtat gaaaaacgaa aattttaaga
    19381 atattgcttt ggataccttg aaaaaacagt tggaaaaaca aaccgcagtt tcttggacaa
    19441 cctgattagt ctggttaaaa tgacaagcat cggcaaaaga aacttgtggc ggttatgcta
    19501 cactgtaaag cgaagactaa aaaggaggaa aagggatgcg tacacttatc atcattagcc
    19561 atccaacgat cgaagaatct ttaaatcagc aatttttcaa agaagcttct gctggtttac
    19621 cagctacttg gcatcatttg gaatcaagtt atccagacgg aaaaatagat atagagactg
    19681 aactacagct cttggcccag catgatcgga tcattttcca gtttccgttt tactggtata
    19741 gttctcctgc tcatttaaaa atttggcaag ataatgtctt ggaacgagca gcacatgtat
    19801 taaaagggaa agaactagga atcgtattga caacaggtgt ggcggaaaaa gaataccaag
    19861 ctggaggaaa agaagaatat acgatttccg aatttttgag gccgtatcag cgaatcgcaa
    19921 ataaattcca tatgacctat cttccgccat tcgttttagc acaatttatg tatttatccc
    19981 aggaaaaacg ttgggagaag ctaattgctt atcagcagta tctctcattg gaaggcaaac
    20041 catcgctgac tcagcggatt gactggttta ttcaaagagt acaggaaaat cagaaaatgc
    20101 aggaagaaga atctgaaaaa caagcatata tcgtagaagc actgactgag gcaaaagagc
    20161 agatcgaaga cttgaccttt acactgcaag aaatgaaagg aacttcatta tgacaaatga
    20221 ttggctgatc caacaattag atgaattaag cgaaaaatca gaatatcaag accgtgcgct
    20281 cttatatgaa ataaagaaac tgatggctga gatagacgaa cgaacaatgc agctgcaagg
    20341 agaaatcgac ggacaatctt ggaatcatct caactggtga aattttctta taaataacaa
    20401 agataaaaat gtgcacagcg acaattattt gctacaataa gaatatgaag cgtttgataa
    20461 cgctattata cataaaaatt ggtggtgaaa aaaatgtatg atgaaaatga gtggggagaa
    20521 aatgttcgga tgacccgaag aagacagcaa aaacaggcag ctcgtaagcg gatgatcatg
    20581 attggggcag cagttgccgt gtttttgatc atagcaggag gagttgcggg tctatatttt
    20641 catggaaaaa attcgtcaga agcgaaaagt acgaccataa cggaaacaac gacaaagaaa
    20701 acagagacga aacaaacaag tgagtcatca actaaaacaa aagagacaca aacttctgaa
    20761 gaaaaaacag caaatggttt gccagagcaa cttcaagcaa tcgtaaatga ttattcgaaa
    20821 aagatcgaag aaaagacgcc tgcattgatt gaagaatacc agacagaaat tcagggaaat
    20881 caagagggaa tagctggttt atcagcagtt gctaaccaaa aagcacgtga gcttcaagca
    20941 atctccgacg aagggatcag aaaattacgt gcagcgtatc aggcagcaga aaataaagac
    21001 ggtgtagact tagatacttt gatcaatcaa ttatcggcta attatacgaa tcatgtcgct
    21061 aagatttccg atatttattt gcagactagc gcctctcttc aagcagagtc aacaagcaca
    21121 caagacacaa catcttctga ttctgagaca gcagagtcgg tagaagcttc tgaaatgcaa
    21181 gctcgaacaa cacaagacac aacagattcc tctgaaacta ataccaacag cgcgacttca
    21241 caggctgcga caaccgtagt tttggaagga gaaggtccaa atcaaatagc cgaacgaact
    21301 ggcgtaccag tagaaacgat cttgtcgctg aatgggatga gcatggagaa ttatttcctg
    21361 actcccggtg agacgctaaa attgaattga taaaagcaat aaaaaagaac gaagcttcca
    21421 ctaaggtgaa gcttcgttct ttttttattt tccggttctt ttttcgcgta caaagtaatg
    21481 gatcgtcatg taaagcaaga aaacgagtcc aataatcaat gagattctcg tttcaggatt
    21541 gataaacatg aaaatcaata cgatagctaa aaaagcaagt gtcacataat tcgtataagg
    21601 agatagtggc attttaaaag gatgatcatg cattttatct gcgtgcttct ttttgaagcg
    21661 aatctcgctg atcaaaatca ctacccaagg aaccatacca ggcaaaatac ttgagctgta
    21721 tacgaaaacg aaaatatttt ctgactggtt gatgaacatt ggtagaagat aatttaagat
    21781 cagaccaatt aagattccac ctgagattgc tacgacagga taaaaaggcg caccgtgacg
    21841 agatagtttc agaaattttt tagacatctt tccatttatg gcaagtgtat agaccatacg
    21901 acttgcgctg aaaatccctg aattgcaacc tgataatgcc gctgtaatca cgacaaaatt
    21961 aatcagtcct gcagcaaatg taatgccgac tttagaaaaa gtttgtacaa aaggtgaacc
    22021 aatctcactc aattgattcc acggatagat actcacaatg ataaaaatag caccaatata
    22081 aaaaatcaag attcgaccga tagtggactg gacagcttca atgatcgtct ttttaggatt
    22141 ctctgcttca ccagcagtca ttccgatcag ttcgattcct tgataggaag caacaacgat
    22201 cgataatgca aagaaaaatc ctttgacgcc tccagtgaag aatccaccat ttttccaaag
    22261 atttgaaatc cctactgcct gaccatgatt accaaatcca aataagatta ccccaagacc
    22321 tgcaatgatc atcaaaatga tcgttatgac tttgatgatc gaaaaccaaa attctaattc
    22381 gccaaacgcc ttgacagaaa taagatttgc cagcataaga aacaagacga caatgattcc
    22441 gggaataatt gccggcaaat caggccacca ataattcata taagatccta cagcaatcac
    22501 ttcacttatt cctacgacaa tatattgaaa aatattactc caagcggtca agtagccagc
    22561 cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa ccagtcgaag gatcaacgta
    22621 aagcatttcg cctaaagcac gcataatcaa atacaaaaag aatcctgcaa tcgcataggc
    22681 tcccatcact gagggccctg tccaacgtat ggttgaggag gcacccataa acagaccgac
    22741 accgatcgtt ccacctagtg cgatcatttg gatatgacgg gcactaagac ttcgagttaa
    22801 cttttcttct tccatacaaa aactcctttc gttgttgagt caacaagaaa aaataatcaa
    22861 acgtttcaac acatgttttt cattattagg ttagccttat tgaactcttg atgtgattct
    22921 acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa actttttcat cattctactt
    22981 gaaaatgctt tccattttag ttaaactata tttaaataaa aagttaggta aacctaaaaa
    23041 ggagtgtaag atttggaacg tacggccatt gtaattcaag acctttcagc cgcttatcaa
    23101 gggaaaactg ttttgaaaac tatcaacttg acgataaatc cacaaaaaat taccggtatt
    23161 atcggaccaa acggtgcagg aaaatcaaca tttatgaaat ctttgctaga gttgatacca
    23221 gctactactg gtaaagtcac cttttctgga cagtcggtaa actcagtccg taaaaaaata
    23281 gcctacgtgg aacagcgaag tgaattggat ctttcctttc caattgatgt attaggcgtt
    23341 gtacttttag gaacatatcc atctttacga attggacaaa gacctgggaa aaaagaaaaa
    23401 gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag aatatgccaa aagacagatc
    23461 agcgaactat cgggtggaca gcttcagaga gtttttattg caagagctct agcccaagga
    23521 gcagaatgga tctttttaga tgaaccattc gtagggattg atgcgttaag tgaacgaaag
    23581 atctttgaca tcttgcagga attgaagaat tcaggaaaaa cgattttgat cgtccatcat
    23641 gatcttcata aagtagaaga atatttcgat gaggttattc ttttaaataa acagctgatc
    23701 gcttccggtc cagtacaaga agtctttaca tcgaagaacc ttcaattggc ctatggtgag
    23761 atcatcagac atttagtaaa aggaggagag aaaaaatgat ccaatccttt attgatggat
    23821 taatgaatta tcaattcctg caatacgctt tggtcacttc catgcttgta ggacttgctt
    23881 ctggagtcat cggttctttt attattttac gagggatgtc tttgatggga gacgctatct
    23941 ctcacgcagt tcttccaggt gtagccattt cttatatgtt tggttttagt tacatctttg
    24001 gagccactgc ttttgggatg ctaacagcag cagtgattgg tttcgtgact cagcggagcc
    24061 gattgaaaaa tgatacagcg attggaattg tctttagctc cttttttgca ttagggatta
    24121 tcttgatttc atatgcccag agtgcgacag atttgtatca tattttattt gggaatgtct
    24181 tagctgttag ggaaagcgat ttgttgctga cagcactcgt tagtggaatc gtcttgatat
    24241 ttgtcttttt cttttataaa gaattgaaga tcacatcatt cgatccgaca atggcaaaag
    24301 cttatgggct gaacacatcg ctgatccatt atcttttgat gttctttttg acattagtgg
    24361 ctgtagtcag tttacagaca gtaggaacaa tcttggtgat tgccatgttg atcacaccag
    24421 ccgccacggc ttacttgcta acgaaccatt tactgaaaat gatcattaca gctgcaggaa
    24481 tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta tagttacaat tggccatcag
    24541 gagctacgat cgtgttagca tgtaccgcat tttttatcct tgctttttta ttttctccaa
    24601 caaaaggaat tttatttcag aaaggaagca gatagatgaa aacaaaaaaa tcactctttt
    24661 taatcttggc agttagtttc ttggtattag ccggttgcgg aaagcaggct tctgaccaag
    24721 cggatgaagg aagtaaagaa aagttgtcag tcgtggctac caattcgatc ttggcggaca
    24781 tggcaaaaga ggtcggaaca gatcaaatag atatccacag tatcgtacca gtcggaacag
    24841 atccgcatga atatgaagta ctaccagaag acatcaaaaa ggcaagcgat gcagatgtta
    24901 ttttatacaa cggtttgaat cttgaaacag gtaacagctg gttcgataac ttgatggaaa
    24961 cggctaaaaa agaagaagga aaagattatt ttgcagttag caaaaatgta gaacctcttt
    25021 atttaactag cggtgaagaa catacaaaag cagatcccca tgcttggcta gacctatcta
    25081 acggaataaa atatgtggag gaaatcgcac gtatattctc tgaaaaagat gcagaaaatg
    25141 cgacactcta taaaaaaaat gcagaagcat atgtggaaaa actaaaagaa ttagatacac
    25201 aagcgaagga aagttttgct tctatcgaag agaacaaaaa attattagta acaagtgaag
    25261 gggcattcaa gtatttttca cgagcatatg atttgccagc agcttatata tgggagatca
    25321 atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat cattgatcag ataagagcaa
    25381 aagaagtacc ggttttattc gtggaaacca gtgtagactc aagaagcatg gaacgggtag
    25441 caaaagaaac gggtttgaaa atctatgata aactgttcac tgattccata gcaaaagaag
    25501 gagaacaagg agattcttac tatcagatga tgaaatggaa tattgaaacg atccatgaag
    25561 gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct agcttgagga gcagaccttg
    25621 cattttttat aagagatatg aaaaaaaatt atgctgtttt tcgtcgtaca gatacctttt
    25681 ttgaagaaat tccgttatag ttagagtaag gaaggaaggt gtggctatga attttacacg
    25741 taaagataaa tatacgatta aagaagaagt ctttcgattg atcagccagc aggaaaatac
    25801 cgacaaggat caagaccttc aatttcttga agcagaatta gctaaattat tcatagcaaa
    25861 tcaaaatgag acaacagaac cagatgaaga gctgtacata ggcaaatggt tgtttgatga
    25921 atttcagcct ttagatgaaa ataccggtat cggttatttt gtgttccttt ctccagtcgg
    25981 cttcacacct ggcgtattag ttaaacaaaa cgacgaaata accgtcaaag catggcctgc
    26041 attattttca gtcatcaaag cagaaggagt aatcaaaaag atccagtcgg cctattataa
    26101 aagtcaattt attgccaaca gcaaattaat gaatatgttt tataatcgtc tagtagaaat
    26161 tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca cagcaaaaaa atctctatga
    26221 agaagtattg tattcccgcg atctgtctga gttttcgcca tgggaagagg taaggctcca
    26281 tgaactgtta agtacaaaac cagtttcatt gaattttttt aatgcattag ataacaaaag
    26341 aaaattaatt tcaacaaatt attgggaagt atttgctgca agaaaaaaat ggatggaaga
    26401 aaagaagatt gccgatcagc agatccagtt tgtccagctg aaaaaagaaa aactatatta
    26461 tgggttatac agccgttaac agcaagcgag gaagaccatg acattaaaag aagaaatcat
    26521 acaagaaagc aaacgattag gaattgacaa aatcgggttc accacggcgg aaccttttga
    26581 ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc catacttctg gctttgaaca
    26641 tccaaatatc gatgagcgtc tttatccaga aatgacattt gatcagccga aatcgatcat
    26701 ttccattgca cttgcgtatc ctactaggat ccatgaagaa gtgcctaggg atgaaaaacg
    26761 cgggcagttt gctcgtgctt cttgggggat cgattaccat gatatactca gagaccgttt
    26821 gaaccgattg atcgaattca tccaaacaag agcagaaaaa tggcaaaaag aagaggaatg
    26881 gcgtctagct ccgcaagtag atactggaga actgatcgat gtcgctgtag cacagcgagc
    26941 tggtttagga tttattggac gaaacggatt gttgatcact gaagagttcg gttcatttgt
    27001 ctatcttggt gagatcgtta ctaatattgt atttgagcct gataaaccag gagaatttgg
    27061 ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca aaagcattgc ttggtgacgg
    27121 aagaatgaat gcccagcgat gtttgtctta tcaaactcag acaaaaggga tgatgccgga
    27181 agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt gatatctgcc agttagtctg
    27241 cccttacaat caaggaaaag attttcattt tcatgaagag atggaaccaa aagtagacga
    27301 aatatatcca aaattaaagc caatgctttc gatgtctaat aaagacttca aacagcaatt
    27361 tgggcattta gctggttcat ggcgtgggaa gaaacctttg cagagaaacg cattgattgc
    27421 tttagctaat ctaggtgatc gaacggcatt gcctgagatt tcagaatgtt taacagatgt
    27481 ccgccctgtc atcagaggaa cagcagcttg ggctattgga cgtctaggaa caaaagaacc
    27541 ggaaaaatgg cttgatgtat tagatgaaat attagctaaa gaaacagaag aagtcgttat
    27601 aaacgaattg gaacatgccc agaaattatt gcggagaaag ttaaaataaa aaaatagtta
    27661 acattattca aagtttttgt ccagtaattg ttgtaacaat tactggtttt tttgtatttt
    27721 ttacattttt ttaagagaat ttgataaaaa tcaaaaatt
//
LOCUS       DAZKNO010000016        27684 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000016 DAZKNO010000000
VERSION     DAZKNO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.016"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..1192)
                     /locus_tag="ACG8MY_000515"
     CDS             complement(248..1192)
                     /locus_tag="ACG8MY_000515"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0429744.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1488..2432)
                     /locus_tag="ACG8MY_000516"
     CDS             complement(1488..2432)
                     /locus_tag="ACG8MY_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0429745.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2653..3867
                     /locus_tag="ACG8MY_000517"
     CDS             2653..3867
                     /locus_tag="ACG8MY_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0429746.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3889..5904
                     /locus_tag="ACG8MY_000518"
     CDS             3889..5904
                     /locus_tag="ACG8MY_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HFQ0429747.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5987..7360)
                     /locus_tag="ACG8MY_000519"
     CDS             complement(5987..7360)
                     /locus_tag="ACG8MY_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0429748.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7386..7670)
                     /locus_tag="ACG8MY_000520"
     CDS             complement(7386..7670)
                     /locus_tag="ACG8MY_000520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0429749.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7672..9687)
                     /locus_tag="ACG8MY_000521"
     CDS             complement(7672..9687)
                     /locus_tag="ACG8MY_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0429750.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIKKGDE"
     gene            complement(9866..10171)
                     /locus_tag="ACG8MY_000522"
     CDS             complement(9866..10171)
                     /locus_tag="ACG8MY_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0429751.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10260..11291)
                     /locus_tag="ACG8MY_000523"
     CDS             complement(10260..11291)
                     /locus_tag="ACG8MY_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0429752.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11498..12373
                     /locus_tag="ACG8MY_000524"
     CDS             11498..12373
                     /locus_tag="ACG8MY_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0429753.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12525..13367)
                     /locus_tag="ACG8MY_000525"
     CDS             complement(12525..13367)
                     /locus_tag="ACG8MY_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0429754.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13459..15336)
                     /locus_tag="ACG8MY_000526"
     CDS             complement(13459..15336)
                     /locus_tag="ACG8MY_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0429755.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     RPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEATIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIDNQTNTPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDHETSGTYAYVLYDG
                     DKEIDPTNYSVTEQTNGFTVSVDSNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15333..16754)
                     /locus_tag="ACG8MY_000527"
     CDS             complement(15333..16754)
                     /locus_tag="ACG8MY_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0429756.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16757..20146)
                     /locus_tag="ACG8MY_000528"
     CDS             complement(16757..20146)
                     /locus_tag="ACG8MY_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0429757.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20403..21794
                     /locus_tag="ACG8MY_000529"
     CDS             20403..21794
                     /locus_tag="ACG8MY_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0429758.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21867..22514)
                     /locus_tag="ACG8MY_000530"
     CDS             complement(21867..22514)
                     /locus_tag="ACG8MY_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0429759.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22522..23208)
                     /locus_tag="ACG8MY_000531"
     CDS             complement(22522..23208)
                     /locus_tag="ACG8MY_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429760.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23214..24287)
                     /locus_tag="ACG8MY_000532"
     CDS             complement(23214..24287)
                     /locus_tag="ACG8MY_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429761.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24509..25183
                     /locus_tag="ACG8MY_000533"
     CDS             24509..25183
                     /locus_tag="ACG8MY_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0429762.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25188..26528
                     /locus_tag="ACG8MY_000534"
     CDS             25188..26528
                     /locus_tag="ACG8MY_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0429763.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26677..27663)
                     /locus_tag="ACG8MY_000535"
     CDS             complement(26677..27663)
                     /locus_tag="ACG8MY_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0429764.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8064 a         5249 c         5025 g         9346 t
ORIGIN      
        1 atgaatcatt gtatttgccg ttgcggaagg attttttaat taaccacaga taaacattga
       61 taacgattcg cttattgtaa tcaacagatt aaatttcata tgttcatatg tacggataca
      121 ttttcggacc gattaatttt aaccattctg tttatttgct tacctattag atatcaataa
      181 aaaactgctt aacagctatt tcagctgtta agcagtttta ggatttttag ttcatattca
      241 ttgtttctta taatacagtg ttcaatactt ctcttaaagc acttgttgat ttgctgaaca
      301 ttgctttttc ttcttcgttg attgataatt caacaacttc acgaacacca ttttcgtcaa
      361 cgattgatgg cacacctgta aagatatctt tttcgtcgta ttcaccagtt aaataagcag
      421 atacaggcaa tacagcttgt tcgttattca agatagcttt tacgatacgt gcagtactca
      481 taccaatacc atagtaagtt gcttttttac gatcaatgat ctcataagca gcattacgga
      541 ctttatcagc aatcacatct aattcatctt gggcaatacg atggtctttt tctacaattt
      601 caaacactgg tttaccaccg acagttgtat gtgaccatgc agcaacttct gaatcaccat
      661 gttcacctaa gatatagccg tggacgctgc gtgggtcaac ttttaatttc aatgcgattt
      721 ctttacggaa acgagttgta tccaatgttg ttccagtacc gataacacgt gatgttggca
      781 gaccagattc gttccaagcg atatatgtca aaatatctac tgggtttgaa gcaacaacga
      841 taataccgtc gaatcctgat cccatgattt cttttacgat ttggcgcatg attgacgcat
      901 ttgttttaac taaatctaaa cgagattgac caggtttttg gttgatacca gctgtgataa
      961 caacgatatc agcatcttta cattcttcat agccacctga ccatacagcc acattttcat
     1021 cgccccaagc cataccatct aaaaggtcca atgcttcccc ttctgctttt tcttgattca
     1081 catcgatcaa tactaattca ttagagattc cttggttgat cattgcataa gcgatacttg
     1141 ttccaacaaa acctgtaccg acgattacta ctttacgact tgtttttttc atgaataaac
     1201 agctcctttt cataactatt tgttactttc atgaaaagta tagtcttgtt tattcatttt
     1261 atcaacatgt taattataat agttgttatt ataatttttg atggaaacga attcctgaat
     1321 acgcataaca gataaatgac aaatgtttac acctatatcc cctgataata caaccaaaag
     1381 tttagaactg aaaataaatc caaaaaaatt cgctgcaacg atataataag acagcaatcg
     1441 cacctaaaac aattttcctt catatgcttt agtgaaagat tttcttctta atgtttattt
     1501 ctccattcat tgggagtaca tgttgaatac ttcttgaata atttgaataa agaagtcgtt
     1561 tgtttatatc cgatacgatt agcgatttct tccaacgaaa aactcgtttc ttgaatcagt
     1621 tccaacgcaa agtcgaattt tttctttaga accagttctt gaaaggaagc atcaaaatac
     1681 attttcaatt tattactaag atagaccgga ttgtagccaa aatgagctgc tgtttctttt
     1741 aacgtacaag aagtaaaatg cttatcgata taatctaata ctctaagcat ctcattttct
     1801 gcgcatccat ctgctgatga aaagctttgt tgttctaata aatgagtcaa ttcgatcagt
     1861 aatagtgatg tatagagatt taacgaacgg atcttatgac tgtatgtatc aaaataaatc
     1921 aacagcatat tttcaatcaa atgcgtcaaa cgacttgttt ttccgccgcg aaaatgaatg
     1981 aattgtgtat gttccccttt ttcttttgat gcatttatca aaaaagaaga aagtagatta
     2041 tttgtgcttg ccaaatgatt caatatctct gtagataagg tttcttcttt taataagatg
     2101 ttgattaaaa tatcctcttg ccctgtgtat tcgattcttt gagtagcttc tttatcaatg
     2161 atcaccacgt cactttcttc catctctatc ctttctccat taataatttg taaacatgta
     2221 ccgctataca tataattgat ttcaacaaaa tcatgtgtat gctcgggaac ttccgcatat
     2281 cgacggtgtt tactgatatg aatatttccc tgttgaaata gatcccctcg gtggattttt
     2341 ggcggtgtct gaagatggta agctaagttt aaatcattga tattcattcc ttgttcacgc
     2401 tgcagagttt cgattgattc ttcgttacgc aaaaaaaatg tctaattcag ctagttccaa
     2461 ttgactccct cctttttgta agcggtttct ttcgcttatt agttcacgct gtactgctat
     2521 tatatgcaaa gctatttttg gtttcataaa gactttctat ggttgggaca tgaacttcct
     2581 gtgattccgc tattgagaga ataaccactt catactatat ttttgataag cgcttacaaa
     2641 aatgaaagga acatgcttat gaataaaaag atgatgaaat tttctctgct gtcagtctct
     2701 cttattttga cttctgctgg tgcagtcacg tctgcaattc ccagtatgtc agctacttat
     2761 gccacgcgtt ctttatctgc tgttgaatta ttaacgactg ctccctcttt tatggtcact
     2821 atttttgttt tgattagtag ttttattgct aggaaaatcg gacaaaaaca aactgtgatc
     2881 atcggattag tgattgcatc gatttatggt atcctccccc tcttctcaca aagttacagt
     2941 attgtcttag cttcaagagc tggactaggt attggctttg gattgatcaa ttctttggca
     3001 gtcagcatga ttgctgcctt ttatcacgga gatgaacgag cctctatgat tggttttcaa
     3061 agtgcatttc aaggatttgg tgcagcttta atgacatttg ttgcaggaca gctagtacag
     3121 ttcggttggc aatatacctt tttagtctac gccatcagct tacctattct tgtcttgttt
     3181 gttttgtttg ttcctacacc tgaaacaact gaacttcaat cacatggcac agccccaaca
     3241 agacaagtaa tcaaaacgaa tcctaaaatg attagttatg gtatttttct cttgctggtc
     3301 atcgtggtat ataatgctgt atcgattaaa ttagccacag tcctgacgac ttattctcta
     3361 ggaaacgaaa ctaatgcagc gaatatctta acaatcatgc aattggcaag tatggctgcc
     3421 ggagtttgtt ttggctggct tcagacaaaa gcacatcgct atatgcttac actttcttta
     3481 ctactgatgg gaatcggctt tgtattgatt gccactgtga taaacctgta tgtgattggg
     3541 tttggtgcga tcctaacagg aatttctttt tcgttattca ttccttactt attcaaccaa
     3601 gtaagtatcc aagcgcccaa gtacgcggag agtttcaata cttcaatctt attggttggc
     3661 gctaatttag gtactttcat tgcaccatat ggtttgctct ttctctcttt tgcttctctt
     3721 gggagtaaaa cacttgctgt tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt
     3781 attagccttc tagtagtaca caggacgaat agaaagacag tgataagagt agatgaagaa
     3841 ataaaagaac aattgacaac aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa
     3901 aaacataaag atagatggct ttggtatcct ggagactttg agatccgcca tggactgcta
     3961 caaaatttcc aacgggaaga acgaggtttt gactggccag cgtactggta catggatgat
     4021 tgccatcgga acgtcaagtt taagagatat tactttcttg atcaaccttc catgtttaaa
     4081 gtgacgatcc aaggagttgg gtatgtagaa attaatggac aaaaacatcc atgtggaaaa
     4141 tggctgactt gtccagctgg taaagcaaaa attcgtatct ttgtaggaca tacatcaggg
     4201 ctaccagcga tgtttatcga gggagacgaa gtaaaatcag atattggctg gacagcaagt
     4261 aattttattg aagaatatcc agctggctgg agtccgcttt atgtcgatat cgcaaaagat
     4321 ccaaatcaaa tctattatca aaaagaatgc gtccatccag tcactgaaac ggagattggc
     4381 ggaggaatct tattcgattt tgggcgagca gtgaatggct gtgtagaact aatatctctg
     4441 gatgaccata tcgaatctat cactctatgt tatggagagt ccgatgtaga agctatggat
     4501 atcgattatt gctactataa gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc
     4561 gcttttcgct atttgtttat accagaggtt tctactggaa tggttcaact tcaagttttt
     4621 catgaatttt tatctctttc gaaacaagct gctttttcaa gcgattcttc tttactgaat
     4681 cagatctggt cagtatcaga agaaaccttc aagttatgta gtgatttgtt ttttatcgac
     4741 ggtatcaaac gagatcgctg gatttggtct ggagatgcgt atcaatcatt catgatcaat
     4801 caatatcttt tttttgacga ggaaatcaat aaacgaacga tattagcatt aagaggacaa
     4861 aatgaaatca agcagcatat gaatactatc gttgactatt cgatcttgtg gttgattggt
     4921 atcgaaaacc actatatgat gacgaaagac tatgaatttt tacgcatcgt ctaccctaag
     4981 atgcaatcat taatgagata cttgatggag caaacgaatg aattagggtt tatttatggg
     5041 agagcaaaag attggatatt tattgattgg tctgaaatgg ataaagaagg aacgcttgct
     5101 gcagaacaag tcttattact gaaagcctat cattccatga tcacttgcgg agatatctta
     5161 gctgaaacat atggagagaa cgaaaattat catactcatc tctatcaaga aaaatatcaa
     5221 ctattgaaac aaaacctatt tgactacttt tgggatgaag aacttggcgc ttttatcgat
     5281 tgctatgaat ctgggagaag aaatgtgacg cgtcatgcga atattttcgc tgttttattc
     5341 gattttgcta acaaagacca acagcaatcg atcttaaaga atgtcttatt gaatgacact
     5401 atcacacaga ttactactcc atatttcaaa ttctatgaac aagatgcttt atgcaaactc
     5461 ggacagacga atatcgtcta ccaaacgatt cttgattatt ggggagggat gttatctcat
     5521 gatgcggtaa ctttctggga agaatacgac cctactcaaa caggaaatga aaagtacagt
     5581 atgtatggag atccttatgg aaaaagctta tgccatgcat ggggagcaag cccaatctat
     5641 cttttaggac gctattttat cggtttgtat ccaacaaagc caggttatga agaatttatg
     5701 atacaaccta atctagattc atttgaaaca ctgaattgta cgctacctat caaagatgga
     5761 aagatttcgc ttgaaaaaac aaaagataag atcgtgatta cttctgatag aacaggcgga
     5821 acagtgaaaa tgaatggtaa aagttatcct cttctagcaa aacagccact tgtcttaaca
     5881 agctattcaa aaataaatgc ataaaacaaa aaactgcttg cgacatatca gaagacttta
     5941 aatcttcttc tgatattgtc caagcaattt ttattttgaa gtttgcttaa ttctgttctt
     6001 tagaaacaat gtttgttctt tttccggcaa aaagaaagct agataatacg acaagtaaaa
     6061 agaaagcgat gattccaact gtcaaggcta tttgaccgta ttctcctagt ttcccaagta
     6121 caagtcctgg tactagataa tcagtatcgg caaatgtaga agaagcgatt ttaagatctc
     6181 ctaatactgg aaggataaat aatggcagcc aactaatcaa taagctattg aaagcagaac
     6241 ctaataccgc ccctcttcta ccgccagtcg agttgccgaa gacaccagaa gcagctccgc
     6301 agaagaaatg tcctactacc ccaggaatga taactgtagt ttgcaagcct atcatagcaa
     6361 acatggatag tgttcctaca aagaagctaa caaaaaagcc aattaataca gcattaggtg
     6421 catatggaaa aacgatagga acatctaatg ctggtttaga atcgggtacg agttttttag
     6481 caatcccttg gaatgctgga acgatttctg ccaacaccat tcgaacccct tgaaggacga
     6541 taacaaaccc agctgtgaat gttccagctt ggatcagtgc aaagattaca gcgttgtctc
     6601 cattgcttag ttctctttca acaaatccag gaccagctaa caaagcaagt gcgacatata
     6661 cgatcgacat caagagcatg atcgatacag tagaatctcg caaaaagctg aaacttttag
     6721 gaatctttat gtcctctgta gaacggtctt tgtttccaaa aaactttccg atccatccac
     6781 tagaagcata accgatattt ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt
     6841 tcttcataaa cggttgacaa agagcaggcg taatagttaa tagtgttcct tcgaagaccc
     6901 cgcctaataa gatcatccac aaatttgcag taaaccccgc gcttactaaa atgactgctg
     6961 ttagacaaga aacataaagc attgcttgac ctgtaagaaa aatgaattta tatcttgtga
     7021 atctagcaag taaaatattc acgatcatcc ctactagcat gatcaaagca gtaggtgtac
     7081 cgtattgttg caaagcaatc gccactactg cttcattgct aggaactact ccttgagtat
     7141 gcagagctgt ttggaacatc gaagcaaaag gggttagtga attactgatt aaaccagcac
     7201 cgccgatcag tactaggaat ccgacaaatg ttttgatagt tccttggatc acttctgaaa
     7261 atttcttctt ctgaagaacg agaccaagca gtgcaatcaa agaaacgagg atggagggct
     7321 gactgacgat tgacacaaac aaattcaaca atttatccat tgatagttcc tcccttgaat
     7381 catttttatt cttttgacaa gatcttcgtt actttttctt ttacttcttc gctgtcaatg
     7441 atcgattgca aaggtacgag cttttcatca ggtatagaag ttagcgtatc tttcaaagaa
     7501 tgctcaatga agaaataatc agccatatcg ctagatacac ttccaacatc cgtatgactt
     7561 acttcaaact cgtccacatc tgctccaaga tctgcaagtg cttgtttgat gttcatctct
     7621 accataaaac ttgttcctaa tccactttga catactgcaa aaattttcat atcattcgtc
     7681 tccttttttt atgatcgcta atatttcttc ttttgttgat gcgtgtaata aatcttgaag
     7741 attttgttta ttagaaagta gctttgttaa atttgccaaa gcccgaagat gtgcctcatt
     7801 atcgatagca gctaaacaga tgaacaaact aatgggatgg gctggattat caagcagatc
     7861 cacaggttgt tgaagtttca gcaaagacat ccctacttcg tttacgccat cttctggacg
     7921 agcatgaggt aatgcgatgt gatcgccaat atggataaac gcaccatact gctccacgcg
     7981 ctcgatcata gctttgatat aactttcttc tattttattt tctgataata gcggctgtgc
     8041 ggcaaatgtg attgcttctt gccagttttt tggttgatcc gataattgga tcatttcttc
     8101 tgttaataat tcagatagca taggacgttg atcctccttc ttttctaaaa gtttgttcat
     8161 tttacgattt aatacttgat agagcttttc ttcgtctacg ccttctttta attcgatata
     8221 aggcagtagc gccgacaaca attcttgggc agaaggcatt gaaaatcctg gtatcagcca
     8281 atctctttgt acttgattga tcagctgatt tttttccaag tcagtcatga ttggatgcag
     8341 aacatagaga gactttttct tatcagtaag tgtgagaggt acagtagaaa aaataatatc
     8401 gtaagaagtt tcgtcaacct cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat
     8461 catcgggaat aattgctgaa gttcactttt taagatcaat gaagagctaa tgccacttgg
     8521 acagacgatc acggctctta tttttctatt tctttcttct gcgtccgctt ttctgatctc
     8581 tccgccaaat agtatagtga aaaaaccaat ctcttcttca gggatgctct tccctattcg
     8641 tctttctaac ggaagtaatg cttttcttgt catttcgtag atggaagcat attgatgctt
     8701 aatttgatcg atcatcacat taggtaaata aaaagaatag gaaatacgaa aataggcagg
     8761 gaccaagtga taaaagacat tcatcaataa ttcacgaaac gaagcaaatt ctacggccat
     8821 caggaattcc atgcgatgga tgacttctga agaacattcc agcaaaaaat caagtgcagg
     8881 ctcctggaca tctccttcaa cgatcgatgc taagcaaaga gagaaaaata taatctccga
     8941 attttgatgt tcggaatagc caaggccagt caatacttgt cgactaatgt cagtgactgc
     9001 aagagaagcc aacagttcag catcttttac cagttccatg gatgtttttc caattcgaat
     9061 ggttgtcaaa ctcaaataat aagctaattc atccattcga tttggtgcga atcgaagatc
     9121 tgatagtgaa atcgttttat ctagcaaatc tctaactttg gcgtaataat caatggaaag
     9181 ggttgcggtt aaatacgtga gcccaaatct tccagcaagt gactgcatca aacgagcagt
     9241 gcgatttttt gcttctcttc ttaatattgc ttctgaccct agcagcctaa aaccagtttt
     9301 cctagtatac gacagctgac aatcttccac ttctaattca tttcttatct ttttgatatc
     9361 tgacaggact gtccctttag aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga
     9421 cgttgcagtc gaataaacca tcaaataaag aagatctctt cgctcttgtt cggtatacac
     9481 agcttcaaat cgagtaatat ccaaaaattt ttctgcccag tcgattgctc ggaatttctt
     9541 tatacggata cgggtatcat ccacatattc ggcaaaagga gttaattgtt tgatcgcctc
     9601 gcttgtattt acttcgatga tctctgctgc tttttctaga ctgatttctc ttggtgtttt
     9661 caggagataa ctaattttgt agttcacagc gctgttcacc tcctacttat attctagtat
     9721 ggtagcgctt ttaaatcgag gggtgtttgg tacaacttac agttggattt tttttacgac
     9781 tgtaccgaac gatttctatc gaatggatac agaaaaaaag caggtgacca aatgtctcct
     9841 gcttttatga attgatttcg tccgatcaat gatgatcatg tttatttttt gaggtcagtt
     9901 cttcaaattt ttctttgaca tcttcaaccg ctttttcgat atctttttca aagtgattgg
     9961 gctcattagg ctttgtttcc tcttctacag aatttgaacc agataaagct gtttctttat
    10021 tttctgtttc accagagttt gtgccagcta atgattcttc tttgtcttct gtttcaccag
    10081 aattagttcc tgctaaagcc acttcttctt ctgttttagg ggccattgaa cctcttaatc
    10141 gtttgtgttt ttcaccgtca taatttgtca tgttggttcc tcctagtagt tctatacttt
    10201 ttcatcgtac aataagacag tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt
    10261 catatttttc cttctgattc ttgaatgctt ttgattcctt cttccaaaat tttcatttgt
    10321 aagattgttt cttcatcttt gatgactttt tctgcgccat taactaatgt ctcgtaaatc
    10381 ccttcgtaga cacggctgta atcaccaacc tcggaaatta ctttttcttc atgataactc
    10441 ccttgttcat ctatataggt caatacacca taatgttcgg gcaaatcgat accaaagtct
    10501 tcatgatcag gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt tgtttgtttc
    10561 acgaacatcc ctttttttcc gtaaacgacg aaacttggac gttctttgat gcggaaataa
    10621 ctggatttta tcgaaatttt catatttcca tagaaaaaat ccagatcgaa gtaatcattc
    10681 attcgtcctt tccctaaaag ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa
    10741 taagaaagaa cttgatctac tgtatgacaa gcatgtccat ataaatagct gttcgcagta
    10801 gaaaattcat gcgtattttc tggtacttcc ggtctgaaat aatcataatg catttctact
    10861 tctaaaatat cccctaattt tccgctttcg attacttttt ggactgtcaa gaagtcagaa
    10921 tcgaaccgtc tgttttggta acattggata aatagacctt tttcttttgc taatgcaaat
    10981 aactcttctg cttcctttga cgtttctgca aaaggtttct ctactagtac attcttacca
    11041 tgttcgagtg ccatttttcc atattcataa tgaaattgac taggtgtagt gatgacaact
    11101 aattggatct caggatcatc ccaaagatca gacagctgat cagtatattc gatgtcttcg
    11161 aatgttttcc acactggttc agatggggaa aaaatttttt tgatattgat tttgttcgta
    11221 agttttcttg aaaaaggcaa atgatagcga tttgtactct ttccattgcc aagataagcg
    11281 attgtaagca tgagtggtgc ctccttttat tgatgttttc agtatatcct agaaaagcgc
    11341 agaaagtatt tataatagct attctggtaa aaaagttcag aaaaacactt gttatcattc
    11401 ggtttggtga aattaatgtt caaagtaagg ataaaaatca aaaagatagt agcggaacca
    11461 agtgtttatc agtaagatag aatttggagg tcagatcatg cttattgaag aaaaactaac
    11521 aaaacaagag ttgtttacca cgacagagaa acggattgtc gattacatcc gtaggaatat
    11581 cgaagctgca gtctacatga cgattgaaga attagcaaaa gctacgtata cttcccattc
    11641 agctatcatt cgtttatgca aaaaaatggg attcagcgga tttaaagaat tccgtttcga
    11701 gctggggcgg gaagttcatc aattgattgc tcagttcaat caaacgattg atcctaattt
    11761 tcctttttca agtgaagata gcactcagca aattgcaaag aaaatggcgg atttatcgat
    11821 ccagtcaata aaaaaagcac aattgcagat tgaacatgaa gatttagatc aaatcggtaa
    11881 agtattgaac aaggcacagc gaatctttct ttttgcaaaa ggtgattctc aaatcacggc
    11941 aaggaaattc cagaataaaa tggtcaagtt gaataagttt ttgatcatgg ctgaagaata
    12001 cagtgattct tcttggaatg cagccaattt gactagcgag gattgcgcca tttttattag
    12061 ctacagcggt cgcatccatc attatgaacg aataatgacc tatttgaagc atgtagaggc
    12121 ccctaccttg ctgattaccg gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg
    12181 tcttgttata tcgcaggagg aatatgattt tgctaaagta gcgactttct cctctcaaat
    12241 tgccttcgat tacgtattaa atacgttatt ctcagtgata tattctcaaa attttgaaga
    12301 gaatatccgc aacttgaaaa acaaacaaga attgatgcaa aaaggccttc ttaaagaata
    12361 tcggaaaact taagaaaaga tcctcattta gtaataagaa agatagtgtt tgataggctc
    12421 tctttgatat catcataaaa acaggctgaa gttttctttt tcaaacctca gcctgtttgt
    12481 ttacggaacg attcgaatag atatattata gaaatgagga atagctaact tctgtttctt
    12541 attttgaacg attgacgaaa aagatatata agaaggagga aaatcagtat acatgcagct
    12601 aacaatccat acaggaaaaa tcgtttgttc tcggatactt tctgtacttc tttttgaact
    12661 tcttttgcat gataaggaac ccggtgccct ctgacaagta agcgatgact gttgaccata
    12721 taaggtgtac aagttaggag tgtcacgaaa tcctgccctt tttcgattct cagatctttt
    12781 gtatcagtcg gttctaccgt cttgatctga tcgacttgat aagcgagtgt ccgtccattg
    12841 atttcgatat agaaagaatc acctaaagtc agtttcggta agtctgtgaa caactttgct
    12901 tgaggcagtc ctcgatggct ggaaataacg gcatgtgtat tttctccccc cgtaggataa
    12961 gacgttcctt ccaataatga ggcacctttt tctagcagca cagtattcgt ttcatcaaaa
    13021 attggcaaat ggacattgat ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa
    13081 taggttctat cctttttcgc aggttctttt ttcttagtaa agggatctgc acctggattt
    13141 ccaccttctt ttgctaattg ctggtttttc tttgtcatgt tttcttggat tttagccatg
    13201 acttcttcat tttctttgac cgcttgttgc tgataatgag agatgatctg ctgatccaga
    13261 taattattca gcgcgtcgct aacaaatgga taagctagta tccctattcc tgagagaagc
    13321 aagaaaagca tcagcagatc tagaagtttc cttttctttg gtttcattcg tgttctcctt
    13381 gtgatgactg tttgataata aaagaaaaag gagcatttgc gtaatccgct gctccttttt
    13441 ccaattcttg caaaaagact aaatctgact gtgcttgcgt ctagcaaagt acagtccagc
    13501 aatcagtaga aggactactc ctgcaccgat atacacatag attcccttac cgcctgttga
    13561 aggaagtgta cctttgtgtt tatttggtat tttttcagga gaaatcaact gtcctgctgt
    13621 tacataagat tgttcatcga tagtaaatgc tacacggttt gttaatggaa catatttttc
    13681 tggcgctttc gtttcttcca gatagtacgt gccatatttt agacctgtca catcgattaa
    13741 accgttactt gtggttgtaa aaaccgttgc tgattctttc gccgataccc agctgacggc
    13801 ttttgtggat gggtcgatcg ataaatattt cgctgtgtca ctatcttgat cacgaacgac
    13861 gaattctgct ccagcaagtg tttgatctga tgtaacgtca ccatctactt taacaaatcg
    13921 tttgccccca gtaacgacat cgactgacgg tggtgtttga tcatttgtat gcccgttatc
    13981 gacatttgct tggttagaaa atcctttggt tggatctgct ttttcgttca aatgcatata
    14041 gtaaacaaat ttcagcgtac caccaggagt gagtgaagga atataattcg agtcaactga
    14101 aaccgtgaat ccgtttgttt gttcagtgac agaataattt gttggatcga tttctttatc
    14161 cccatcatac aagacatacg catacgttcc tgaagtttca tgatcaaatg tcaaagcggc
    14221 gtcatgagta tcaatgagtt tgaatgtcgt gtacttgttt tgtgttcctt ctttatcagc
    14281 gatacctaaa ggaatattga cagagatctc atattggatt ttttctccga ttgcgacatc
    14341 ttttccattc aattgaggtg ttgttttatc aactttggac gtatcatttt tgattgtctt
    14401 ttctacagga gtttgactat ttgccaagat ctcaaacggc gtgtttgttt gattatcaat
    14461 catttccgca ttatcaggag cttttacttc ttctaaatta tattttccaa cctctaaatg
    14521 attgacgatc aactgacctt tttcaatcgt tgcctcggca aagtcattgt tgccgatgtc
    14581 ataagaatga ccagtaatga aatgtttggc tttggtttga tccgttgtcc aagtgtacaa
    14641 tccatctgtg acactttgga tgtatttttt gacgcttggt cttccttctt ctttagaaat
    14701 aataaattct gctccattta gtgcttcgtt ttcggcagta ccgatttttg taactttcaa
    14761 cgttccatca ttaccgactg tatttttagg gtagagatgg atagtatcta gttcttctgt
    14821 cccgtattta taagagccat ctgcttgttt gatcatctca tatacaggga aagctaaaac
    14881 catgtttgcg gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt agactgcatc
    14941 tttcccattt tgttttttag gtaaagataa agtgacattt ccatcagcat ctgtcgttcc
    15001 tgaagcaact ggtgtaccag gagtcaaaga ctggactgct tgttttgctg catccacgga
    15061 cgctccttta tctcgttgcg cataaaattc ttgagtgacg ttataaactg aaaatgaaat
    15121 atcggctaat ccttggtatt gatcgaattc gctcatttct ttcccgctgt tttggattaa
    15181 aggatcgggt aaatcagtca ttttcttttt atgaaggatg acttgagctg ctgtctcctc
    15241 tgcaaacact ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac
    15301 tcctaacata acgtttattt ttttatgatt tttcattttt tcactcctgt tctttttttg
    15361 ataaacaacc agattgcaaa gcttgcgaat accataccaa caacgcctaa aagtgtattt
    15421 ttcgtttcgt ttgtttttgg aagaaatgga cggctatttt cttttttatt gactactttc
    15481 aatatactgt ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa cggaatttct
    15541 gtttcactta aaatataacc agaaggtgct tcgatctctt ttaatcgata acttcctgtt
    15601 ttcaacccgg aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag cgcatctgat
    15661 tcgttctttg tccacttgta tccgttggct gtttgctgga ggtaatttcc tgctttgttt
    15721 ttaataagga atactgcgcc agctaatcgt ttatctggtt tatttgtctc gattttgaca
    15781 aattgcttac tccctgtata tacagagact ttctttgtac tgacaccaaa accaaaatcc
    15841 agtgttccgt tgttgttgat ttccttgttc gcctgtgctg tactgcttag ctgcatctga
    15901 taagaaatgg tcagtttttt tccagcatat ttttccaacg aagggatcga aaataaaaca
    15961 tcaaatccgt gaggtttctt gtgtatgaca aagccttctg tcagcttttc tccattcgat
    16021 gaaatcgtta aactattagg taaaaacgtt aatgcttcat ccgcactatc tgacaattcg
    16081 aacttttgat agtcaaggat atttacagga attgtcgtat gcaaggaata agtgattttt
    16141 tcaccaatcg taaaatcatt tctaggctct tctaatacct tctcaaaagg tggatcaggg
    16201 tagtcgtttt cttcattttt aggatacaaa tggatagttg aaagtttttg attgttttgt
    16261 ccatgtacag gaagaacaac taccatattc tctgcctttt ctttgacgac ttctggtact
    16321 ttggattcaa tgaaaagata cgctttatct cttttttctt gatctttagc ggccagagaa
    16381 aaagaagcga taccatcttc gttgttaaga gttgtagttg ttgcttctgc ggtaaacata
    16441 ccggaagacg caccgttttt tgcgatctct gcttgtgctt cttctaccgt tttgcccttt
    16501 tcccgtagat ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac accatttaat
    16561 cctcgatacg tttgtaataa agctttttct tgtccgtcat ttggatgatt tttcggcagt
    16621 tgcccgtttg ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt ttctgtttct
    16681 gttgctacct gagtaaaagc tggtttaaat agtaacaaga agagacacat actaagccaa
    16741 ccaagttttt tcatatttag ctgccccctc tttctttttt caaaaaaaga taccaaatag
    16801 aaaacgcaca accaatcatc cctactagat agattcctaa acggccaatt cctcccgttt
    16861 caggtaatgg tacttttgcc tgattcgtga tgtctaaaga aatctggttg tttttggctc
    16921 ctggtgacag aacagattca tgatcttgtc catccacttg aattgaccca tcttcgtgta
    16981 taactatagt aactggctct tttagacctt ggtatccttc tggtgtaaaa gtttcggtca
    17041 gcgtataagt tccaggtttt aaattttcga atagaaaggt atctgtttct tgtccatctt
    17101 ttggcgattc taagtccatt tctggtccct gcagtctgaa ttttgctcct tttaatgtct
    17161 gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga ttttgatgag
    17221 tgagcgtcca tacattattt gtcatctcgg tattttttcc cgtgtaaatg atttttccat
    17281 atttatcgat ttggaattca taatttcccg gcgaagtatc atatccggca ggaccagctg
    17341 tttcaacgac tcgatattta ccaggtgtga gtgaatcaaa actggcaaga cctttttcat
    17401 tcgttgtttg gctgtcaatt ggctgataag taccatttgc ttcttttttc tgtagggaaa
    17461 atgctgctcc tttaagattt atctctttgt ccgtcccttg catcgcatac ttacgtactg
    17521 ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca tcggaagtag
    17581 tgactgtttt tccatcaatg gttaccgtac catcagaagc gatcttgatt tcccaagtag
    17641 tctttttgct taatccatgc ccttctggag cttttgtttc tgtcagcgta taggtcattt
    17701 ctttttgcaa tttgacattt tctggaagag aataggttcc gtcgccatga tctgttagta
    17761 aagtatcaat agaatctcct gttaatttga aaacagcgcc aataagatcc ttttcacctg
    17821 tagaggaatt tttcgttatt ttcaagttta acggtgtgaa ttgtttagta tttttccatg
    17881 tcattgcatc tatttgttga gaatcgtatc ctggtacagg taattctttg attgtttggt
    17941 aactgaatgc ttgaccttga ttattgtatt gaggtagtga taatatctct tgataacttt
    18001 ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc ccacgtattt gttgtatctt
    18061 tagctggttt aatgattcga atatatccgt ttttccaagc tgcagcattt gtcgtatgtt
    18121 cccgttgaat ctcaaaagta acttgatctg gacgatcagc tagattgttg tcaaattctt
    18181 cccataactt tttgatgtga agactgactc cgggagcttt agcagatggt ataccgaatt
    18241 cagctaattc attggtatca atgtttggct ggaaagttgt cctgccgttc atttgatacc
    18301 agaaatttgg atggaagtcc tcattttctg tttggattct cacttggtaa tggatttgga
    18361 tttcttggtc ttttcctaaa tagatctgat tgctcttgat ggtatttcct tctatggaaa
    18421 tagatggagt gaccgttgta ggacttgtcc ccacactctt gactgaaagt gtaccaggct
    18481 gataaatgaa tggttctgcg attggatcat ttatttgtcc attgcttaca gttgctgaga
    18541 tctgtacagc ttttttggct aggtattcag agatatctgt tgcatgatca gctgattcat
    18601 agtaaagatc gcctttttca tctgatgaaa ccatttgtcg catacgagac tctacttctg
    18661 cttttgagag accagctgcc ggatcgcttt gaagttggat gccaagacca tgtatttcga
    18721 ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc gataaacgta ctgtcgattc
    18781 ttctggatag attgttttgg tcaggtgcat catagattct tgagattaga gaagtatttc
    18841 ccggccgatc ttgcgtatta gaaaactgag ttccgtaata attgctgctt gattgtgcgt
    18901 gtacccgctg tactttatag gaaaatgttg gtacaccatc cgtcagcaaa acgatcactt
    18961 ttttatgtcc atttggaacg gatagcatgc ttcctgcatc tcttagtgct ttttgtgtaa
    19021 aagtaccacc atttgtccgt gaaggtgtaa tggattttac ttgatttttc actgaatcaa
    19081 atgaacccat ctgtactgca ccgttactgt agctatatcc ttcgcttgag tagccgacat
    19141 atcccatatt gattttgtct gtgataccgc tatctgctaa agtatcgaca aaacgatcga
    19201 caccaatctt tacttcaccg atccgattat tgtcgttcat acttcctgac cagtctacga
    19261 ccaatacgag atcaagaggc gtgatatctt tttgtacatt tccacgagca ttcaaataaa
    19321 catcaaacaa tccgggtgta cttgtttcct ttgcatactt tcgtatcgca tagtctgctt
    19381 gattaccggt tcctccatat tcgatatacg aatgggtccg atcattagga tctccattcc
    19441 aagaatttat accatcccat tgatttgttc cgtttttatt gccttgatga tcaagaacat
    19501 ttgtatttcc tgtaggttgc caactggctt taggataagt gccctgatca tctgttgtat
    19561 attctgtagg aatcgtttga ttagatggca cactcacagc tggcaaggat aatgtaggca
    19621 atgtaaatgg gctgacagaa ttttcgtttt cttttgatga ttcttcttct atttcgtctg
    19681 gctttggtac tgacacatct gtatctttct tattttctgt ctcatcaata atgtcatatt
    19741 ctttattatc gacgatcagc ttgtttgcct ggctgtggta aagtgctgga tcgattggaa
    19801 aactcagatc gatagtttgt ttagtgtttg atgagatttc caacgtaatt ttcccgctat
    19861 ttacctgata agcatagcca tctccttctt ttctttccac atcacttaag taattttcga
    19921 ctagaccaat ttctttttct atcttttcag cactggtgtt attcgcattt agcttgatgt
    19981 tcagtgcttt ttttccagac tgatccgaaa tgacttcagc tgaaattgtt tctaaaactg
    20041 ttggatcatc tgtagtgtct gctattgcag ataatggcac aaagtttgat aaactcaata
    20101 ttatcagcat gaaaataact ttcagtttct tccctgtggt cgtcattttt cccctccttt
    20161 ttcttttttt ctgttttgac gtgaatagtg aaaattatta aaataaacag gcgttacata
    20221 attttattta ttttaatatg ataattatta tacgaagggc gctaaaggga atcacaccaa
    20281 ttttcatacc aattggtttc atttatgaag ttgattcctt cgattaatga gcgtttcatt
    20341 gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt gttggaatga aactatagaa
    20401 agatggaaaa aatcattcga attctgtcca tttctgacaa gtggatgact aaaaaagaac
    20461 ttgccaaacg aattggtagt tcagaatcta catttgtaag gtatatcgaa gagatcaaaa
    20521 tccgctgggg aacggatgta acgattcaat cttcacaaaa attggggtat cgtcttgaac
    20581 atttgaatgt accagcttat ttaaatgttc tgatggaaat gtcgcaaagt tctacaaaca
    20641 gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac gattgagtat tattgtgaaa
    20701 aaatttccat cagccggtct tcttttacac gtaagttgaa acaatgcaat caaattttag
    20761 aaagctattt attaaaagtg atcgttgacc aaggttttca gttaactagc cttaaaagtg
    20821 agctccagtt gcgtatcttt gttacctttt tctttttaag ttactacgga agacgcacgt
    20881 tgccatttcc aatcgaaaaa aagaagatca aagaaattat ggtcaagaat aattgtcaga
    20941 tgaatttgtt tcaggaagaa agcccatatg agcaatcttt ctttattatg tattttgttg
    21001 tgcatctcat gagagaatcc caaggaactc catttgaatg gaaagctgga gactcttatg
    21061 tacagttgat aaatatcgat aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat
    21121 tgaagtacag cagctatata aaagtagtga actattttgt caatacgatt ttttcagaat
    21181 tttcggagaa agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg tcctactgtt
    21241 cgttttcatc cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc
    21301 aaatttattt tttctcccgt attttcccat acgatacgac tcatttgacg atccgtgcaa
    21361 cttactttgc acagcagatg caacaccgcc agatacgtct atatgctgga tttcgtgcag
    21421 aacttcaatt ttattccact ctactgaatc agaatctgat ttattattta tctagctata
    21481 tcttttggat gcttatcggt aatcctgaaa ttcaccatta tacttctgat aaaaaaatac
    21541 tgattatcag cgacttaagt cttcgacata gtcaatatat tgaagaatat atcagtgata
    21601 ttcttgctgt gcataaaatt cactctgaga caactgctat tactgaagat cagctgtcga
    21661 aatacaatct tctcgagtat gatctgatcg ttacgaatca acccattttg aatgcccatg
    21721 ttcctcatat attgattgat gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct
    21781 tattcgagtt atgaataatc aaaaaacagc tatgtataaa ccaccctgat aaaaatttca
    21841 ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca acgagattta
    21901 tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag atccgtcaaa
    21961 gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc cgacgattga
    22021 atggagaaat gattgattct taataattcc agccattttt cgttcattgg agaatcagcc
    22081 gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa gtataacctt
    22141 tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt ttcgatcccc
    22201 aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg gactatgtga
    22261 gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata tttataaaaa
    22321 gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg gctgatgtgc
    22381 tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc gttcgataaa
    22441 ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat acgtggatag
    22501 cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca agtacccatc
    22561 ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag taaccataat
    22621 agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt catatgcatg
    22681 atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct cattatataa
    22741 agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag gataactatt
    22801 tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg aatcttcttt
    22861 ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa
    22921 attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc gttttttttc
    22981 tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg gtgtttgcaa
    23041 tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa tgatgctgac
    23101 gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc
    23161 agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt ttattaacct
    23221 cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa tccgcctagc
    23281 attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa aacagcaaaa
    23341 ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc aagcagaacg
    23401 cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa gaaatttttg
    23461 atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat cacgtacatg
    23521 aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc atttaaggtc
    23581 atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt taataactgt
    23641 agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac gatcccattg
    23701 atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc agtccacgta
    23761 tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa gataccgaca
    23821 atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt atagcctgaa
    23881 tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg ccctttcgtg
    23941 agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt
    24001 tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc ttcaatgtat
    24061 ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa
    24121 acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga aagtccattc
    24181 gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc gacgatcaag
    24241 ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg tttttcctcc
    24301 ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc taaagtgaca
    24361 atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc tttcgataaa
    24421 accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc atgataaact
    24481 aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag aagacgataa
    24541 agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa cacaagttta
    24601 tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg caatcttgga
    24661 tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga aagaaaaaat
    24721 acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga ttgaaggatt
    24781 tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac tgatcatgcg
    24841 cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc tgaagtataa
    24901 ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag ttttgcccat
    24961 acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa tcattttgac
    25021 aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga ttacagttat
    25081 tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg ataaagaaat
    25141 caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg aatggagata
    25201 ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt gcttttatct
    25261 tttttctttt gggattcatt actttacaga tattaaacca atctgcatac agagaaacag
    25321 atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag atacaaagat
    25381 atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga acaaacatgg
    25441 aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta
    25501 aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat
    25561 taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat gaagacagtg
    25621 gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat caagatggcg
    25681 ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta gcgacttttc
    25741 gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt atcagttatt
    25801 acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa aaagaatttg
    25861 ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat agtttggaac
    25921 atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc gctcaagcat
    25981 tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc gctcggaatg
    26041 attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat atcgaacaac
    26101 ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt ctccataacg
    26161 atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg gttattttgc
    26221 tggataatgc attaaaatat acgaatccag aagataagat cattttggat tcatctgtta
    26281 accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt gaagaagatc
    26341 aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa gagactggcg
    26401 gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat ggacagattt
    26461 ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg ttgaagccta
    26521 aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt
    26581 gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttctttaa
    26641 gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat tgcggtcttc
    26701 aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat cttctgtcac
    26761 tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa taactgtatg
    26821 tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat cttccaattt
    26881 ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga cgatttcttc
    26941 acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac
    27001 ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta gttgaattgt
    27061 atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc
    27121 agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg gatccgttgt
    27181 attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc cagtttctaa
    27241 tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg tatcttctgt
    27301 tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag aaatatctcc
    27361 tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat aggtatcaaa
    27421 agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat ccatccgatt
    27481 ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag atgctgcttc
    27541 atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca accgttcaat
    27601 tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat ttttctcttc
    27661 catgtaaatt cctcctagca aata
//
LOCUS       DAZKNO010000017        27251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000017 DAZKNO010000000
VERSION     DAZKNO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.017"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..2696
                     /gene="secA"
                     /locus_tag="ACG8MY_000536"
     CDS             162..2696
                     /gene="secA"
                     /locus_tag="ACG8MY_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0429765.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            2823..3924
                     /gene="prfB"
                     /locus_tag="ACG8MY_000537"
     CDS             join(2823..2894,2896..3924)
                     /gene="prfB"
                     /locus_tag="ACG8MY_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0429766.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4029..4715
                     /gene="ftsE"
                     /locus_tag="ACG8MY_000538"
     CDS             4029..4715
                     /gene="ftsE"
                     /locus_tag="ACG8MY_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0429767.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4708..5592
                     /gene="ftsX"
                     /locus_tag="ACG8MY_000539"
     CDS             4708..5592
                     /gene="ftsX"
                     /locus_tag="ACG8MY_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0429768.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5744..7192
                     /gene="cls"
                     /locus_tag="ACG8MY_000540"
     CDS             5744..7192
                     /gene="cls"
                     /locus_tag="ACG8MY_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0429769.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            7529..8386
                     /locus_tag="ACG8MY_000541"
     CDS             7529..8386
                     /locus_tag="ACG8MY_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0429770.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8501..9421
                     /gene="pstC"
                     /locus_tag="ACG8MY_000542"
     CDS             8501..9421
                     /gene="pstC"
                     /locus_tag="ACG8MY_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0429771.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9421..10305
                     /gene="pstA"
                     /locus_tag="ACG8MY_000543"
     CDS             9421..10305
                     /gene="pstA"
                     /locus_tag="ACG8MY_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0429772.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10320..11126
                     /gene="pstB"
                     /locus_tag="ACG8MY_000544"
     CDS             10320..11126
                     /gene="pstB"
                     /locus_tag="ACG8MY_000544"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0429773.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11142..11900
                     /gene="pstB"
                     /locus_tag="ACG8MY_000545"
     CDS             11142..11900
                     /gene="pstB"
                     /locus_tag="ACG8MY_000545"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0429774.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11913..12590
                     /gene="phoU"
                     /locus_tag="ACG8MY_000546"
     CDS             11913..12590
                     /gene="phoU"
                     /locus_tag="ACG8MY_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0429775.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13154..13846
                     /locus_tag="ACG8MY_000547"
     CDS             13154..13846
                     /locus_tag="ACG8MY_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0429776.1"
                     /translation="MLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAIL
                     FVVIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVI
                     GSMANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIA
                     VLFFLAVRRLKK"
     gene            13843..14610
                     /locus_tag="ACG8MY_000548"
     CDS             13843..14610
                     /locus_tag="ACG8MY_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0429777.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            14710..16278
                     /gene="liaX"
                     /locus_tag="ACG8MY_000549"
     CDS             14710..16278
                     /gene="liaX"
                     /locus_tag="ACG8MY_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0429778.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16454..16762
                     /locus_tag="ACG8MY_000550"
     CDS             16454..16762
                     /locus_tag="ACG8MY_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0429779.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            16762..17112
                     /locus_tag="ACG8MY_000551"
     CDS             16762..17112
                     /locus_tag="ACG8MY_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0429780.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            17278..18216
                     /gene="hprK"
                     /locus_tag="ACG8MY_000552"
     CDS             17278..18216
                     /gene="hprK"
                     /locus_tag="ACG8MY_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0429781.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18231..19061
                     /gene="lgt"
                     /locus_tag="ACG8MY_000553"
     CDS             18231..19061
                     /gene="lgt"
                     /locus_tag="ACG8MY_000553"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0429782.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            19105..20130
                     /locus_tag="ACG8MY_000554"
     CDS             19105..20130
                     /locus_tag="ACG8MY_000554"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0429783.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20145..21083
                     /gene="galU"
                     /locus_tag="ACG8MY_000555"
     CDS             20145..21083
                     /gene="galU"
                     /locus_tag="ACG8MY_000555"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0429784.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            21208..21657
                     /locus_tag="ACG8MY_000556"
     CDS             21208..21657
                     /locus_tag="ACG8MY_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0429785.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            21650..22264
                     /locus_tag="ACG8MY_000557"
     CDS             21650..22264
                     /locus_tag="ACG8MY_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0429786.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(22327..23430)
                     /locus_tag="ACG8MY_000558"
     CDS             complement(22327..23430)
                     /locus_tag="ACG8MY_000558"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0429787.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRSYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            23640..24641
                     /gene="ccpA"
                     /locus_tag="ACG8MY_000559"
     CDS             23640..24641
                     /gene="ccpA"
                     /locus_tag="ACG8MY_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0429788.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            24995..25390
                     /locus_tag="ACG8MY_000560"
     CDS             24995..25390
                     /locus_tag="ACG8MY_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429789.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            25387..26643
                     /locus_tag="ACG8MY_000561"
     CDS             25387..26643
                     /locus_tag="ACG8MY_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0429790.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            26655..26978
                     /locus_tag="ACG8MY_000562"
     CDS             26655..26978
                     /locus_tag="ACG8MY_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429791.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            26971..27237
                     /locus_tag="ACG8MY_000563"
     CDS             26971..27237
                     /locus_tag="ACG8MY_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429792.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         9137 a         4755 c         5936 g         7423 t
ORIGIN      
        1 ttttgagttt cctcctcttt tagatgaaat atgaataaat tatgaagttc ttactttaat
       61 acgcttaaga tatttcaatt atctataact aatgataaaa tgaaaagtaa agtctacata
      121 gtctggataa tacacagaag gaaaaggaga ttacacaacg catggccaat ttgattcgtt
      181 cacttatcga aaacgataag aaagaactaa aacgtttaga tagcattgca aaaaaagttg
      241 aagcatacgc tgaccaaatg gcagcattaa ctgatgaaca attacaggca aaaactgctg
      301 aattcaaggg cagataccaa aaaggagaaa cattagatca attacttccg gaagcttttg
      361 cagtcgtccg agaagcagca aaacgtgtat tggggttata tccataccac gtacaattaa
      421 tgggtgggat cgtgcttcac gatgggaata tccctgaaat gcgtactggt gaaggtaaaa
      481 ccttgactgc aacgatgccg gtgtatctga atgcgttgac tggcgaaggg gtccacgtag
      541 ttaccgtcaa tgaatattta gcaacccgtg actcgacgga aatgggcgag ctttataatt
      601 tcttaggatt gacagttggg ttgaatatca actcaaaatc ttcggatgaa aaacgcgagg
      661 cttataattg cgatatcact tatagcacga ataatgaatt aggatttgac tatttgagag
      721 acaacatggt cgtttaccgt catcaaatgg tacaacgtcc gctgaattat gccattgtcg
      781 atgaagttga ctcgatcttg attgatgaag cgagaacgcc gctgatcatt tcagggcaag
      841 cagaaaaatc aacagcttta tatacaagag cagacaactt cgtgaaacgt ttgaaagaag
      901 aagaagatta taaaatcgat atacaatcaa aaacaatcag tttaactgaa gcagggatcg
      961 aaaaagctga agaaacattc ggtttggaca acttatatga cattgaaaat actgcattga
     1021 cccatcactt ggatcaagca ttgagagcca actacatcat gcttttagac atcgattatg
     1081 ttgttcaaga tggaaaagta ttgattgttg accaatttac gggtcggatc atggatggac
     1141 gccgttattc agatggtctt catcaagcga tcgaagctaa agaaggcgta gagatcgaag
     1201 acgaaacgaa aacgatggcg acgatcactt tccagaacta tttccgtatg tacaagaaac
     1261 ttgcggggat gactggtaca gctaagacag aagaagaaga gttcagagag atttataata
     1321 ttcaagtata ccagattccg acaaaccgtc cggtcatccg tgacgatcga ccagatttgc
     1381 tttacccaac attacaaagc aagttccatg ctgtcgtaca agatatcaaa gatcgctacc
     1441 gcaaaggaca gcctgtctta gtcggaactg ttgcagttga aacatctgag ttattatcag
     1501 atatgctgaa tcaagaacgt attcctcatg aagtactgaa tgcgaaaaat cactttaaag
     1561 aagcagaaat catcatgaat gcgggtcaaa aaggtgcagt gacgattgct accaacatgg
     1621 ctggtcgggg aacagatatc aagctagggc ttggcgtaag agaacttggt ggcctagcag
     1681 ttatcgggac agaacgacat gaatctcgtc gtatcgataa ccagcttcga ggacgtgcag
     1741 gacgtcaagg agaccctggt atgtcacaat tctacttgtc tttagaagat gatttgatga
     1801 aacgattcgg ttcagaacga atcaaagctt tcttagatcg aatgaagatc ggtgacgaag
     1861 atgctgtgat ccaaagcaag atgctgacga aacaagtaga agctgcacaa aaacgcgtag
     1921 aaggaaacaa ctacgacaca cgtaagaacg tcttgcaata cgatgacgtg atgcgtgagc
     1981 aacgtgaagt aatctataaa cagcgtcaag aagtgatcat ggaagaaaaa agcttatcta
     2041 aaccactgat gaatatggtc aaacggacaa tcagtcgtgt ggtagatgcg catacgcaat
     2101 tagaaaaaga aaattggaat ttggaaagta tcgtcgattt tgctggcagc acgcttgtcc
     2161 atgaagacag tatttccctt acagatatcg aaggaaaaac ctctcaagaa atcaaagaat
     2221 atctaaacga acgagcacaa gaagtattca ataccaaagc agctcaattg aacggaccag
     2281 agcaactgct tgaatttgaa aaagtcgtga tcttacgtgt cgtggactct aaatggacag
     2341 atcatattga tgcaatggac caattacgtc agtcaatcgg cttgcgtgcc tatggtcaaa
     2401 acaatccatt agtcgaatac caaacagaag gttataagat gttcgaagat atggtaggtg
     2461 ccattgaata tgaagtcact cgtatcttca tgaaagctga gatccgtcaa aacgtacaac
     2521 gtgagcaagt ttctcaagga acagcttctc actcagaaga tggagatgcc aaagaaaaca
     2581 gtaatactag cgcgaaaaaa caaccaatcc atgtgaacaa ggttggtaga aatgatcctt
     2641 gtccttgtgg aagcgggaaa aaatataaga actgtcacgg aaaagatgca aactaatttc
     2701 tgacagcagg attggtgaga caaattcttt tttaaagaat ttgtctcacc ttctttctgt
     2761 tatttgacga ttctgttaga ctagatataa taaaaaaagt atagtgaaag aaggaaaaaa
     2821 acatggaaat atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag gtcacaagct
     2881 tcaggaggtc tctttgactt agaccagctg gaagaagaca ttgcggaagc agaagcaaaa
     2941 atggctgaac ctggtttttg ggatgatagc gaagctgctc aaaaagtgat cagtgaaaac
     3001 aatgcaaata aagaaactta tgatcaattc cattctcttg ctgaagaact ggaagaaatc
     3061 gaattgatgg tggagatgtt gcaggaagaa cctgatgcag taatgcaaga agaagcagaa
     3121 gcacgtatca atgcgctaga tgaaaaaatg aatacgtatg aattatctat gttgttagat
     3181 ggtccttatg atcgaaacaa cgccatcgtc gaattgcatc caggggctgg cggaacggag
     3241 tcccaagact ggggcagcat gcttttacgc atgtatacac gctgggcaga gcagcatggg
     3301 tatcaagtag agacactaga ttatcaagca ggcgatgaag caggaatcaa gagtgtgacc
     3361 ctactgatca aaggacacaa cgcatttggc tatctgaaat cagaaaaagg tgttcatcgc
     3421 cttgtacgta tctcgccttt tgactcagca aaacgtcgtc atacttcttt ttgttcggta
     3481 gatgtgatgc cagagcttga tgaaacgatc gatatagaaa tcaatccaga tgatttgaag
     3541 atcgacacat atcgggcgag tggtgcaggt ggtcagcaca tcaataagac agaatccgct
     3601 gtacgaatca ctcacttgcc tacaggaaca gtagtagcga gtcaggcaca acgttcacaa
     3661 ttgaaaaacc gggaacaagc gatggggatg ctgaaagcta agctttacca attagaagtg
     3721 gaaaagaaag aacaagaagc agctgcactg agaggagaac aaaaagaaat cggctgggga
     3781 tcacagatcc gttcttatgt tttccatcca tattctatgg taaaagacca tcggacaaac
     3841 gtagagacag gaaatgtcca agctgttatg gacggagatc ttgatttatt tattgacggc
     3901 tatttgaaaa tgcatctgca ataagagccc tttgttaaat tgaaataaaa ttgtaagatt
     3961 ttgcaagcaa gttgaaacat tgccatgcta taatagctaa agactgaaaa agatatggag
     4021 agaacgccat gattgaaatg aaggatgtaa tgaagaagta ctcaaacggt accactgcta
     4081 tccgcaatct ctcgattgtc atcgaacaag gagagtttgt gtatgttgtt ggtccgtctg
     4141 gagctggtaa atctacattc atcaaattga tgtatcgaga agaaaaagct tcaaaaggaa
     4201 ctttgaacgt cgcaggtcat gatctaatag ccatcaaaaa ccgcgaagtt ccttatctac
     4261 gcagagaaat cggcgtggtg ttccaagact ataaattatt accaagaaaa acagtatatg
     4321 aaaatgttgc gtacgccatg caagttatcg gcagaaaacc tcgggatatc aaaaaacgag
     4381 taatggaagt gctggatctt gttggtttga aacacaaagt ccgagtcttt cctagcgaat
     4441 tatctggtgg ggaacaacaa cgtgtttcta ttgcccgagc aatcgtaaat acacctaaag
     4501 tacttatcgc ggatgaaccg acagggaatc tagatccgga aaactcatgg gaaatcatga
     4561 aactattaga tcggatcaat gcacaaggaa caactgtcgt gatggctaca cataacagta
     4621 cgatcgtaaa tacgattcga caccgagtaa ttgctatcga aaacggccgc attattcgag
     4681 accaagcgga aggggaatat ggatacgatg attagaacct ttttccgcca tttattagaa
     4741 agtatcaaga gtttgaaacg taacggctgg atgacagtag cttcggtcag tgcggtaacg
     4801 atcacgttga ctttagttgg tgtgttcatg gcggtcatca tgaacgcaac caaactagca
     4861 caagatatag aaggaaacgt ggatgtatct gtgttcgtag acattggtac aaaagacgaa
     4921 gagatgaaaa aattagaaaa agaattaaat gacttagatc atgtcaaaaa agtcgaattt
     4981 tcaagcaaag aacaggaatt gaagaaaatc caaaacgaaa tgggcgattc ttggaaattg
     5041 tttgaagggg ataataaccc gttatatgat gtatacgtag taagtgctac cgatccttct
     5101 tatacaaaac aagtagcaga aaaagcggca gatttgaaga atgtttcccg ttctgattac
     5161 ggtggggctt cctcagatcg gattttccaa attgctggtg cagttcgaac atggggatta
     5221 gttgcggcgg cattgctgct gtttgtagca atgtttctca tttctaacac gattcggatc
     5281 acgattttat ctagacagcg tgaaattcaa atcatgcgtt tagtaggggc aaaaaatgga
     5341 tatatccgct ggccattttt ccttgaaggt ggttggatcg gcttaatcgg tgccattctt
     5401 ccgatactga tcattacatt tggttacgca tggttttacc aattgatcaa tccgtcatta
     5461 ttacgatctc attattcatt gatccatcca gaaaatatcg tttggaaaat caatcttttg
     5521 atggtcggta ttggtgtgat cattggttca ctcggctcga tcatctctat gagacgtttc
     5581 ttgaaattct agtgtcacaa gataaatata atcagaaagt tggacgattt cgttccagct
     5641 ttcttttttt atcagctagg tggagtaaat ggggcggctt ttttctcttt actttaaaaa
     5701 acaacttggg tattcttaag ttggtagaaa aagaggtgaa gccgtgaaga ttatcgttga
     5761 caattttttt acgatccttt tgattatgaa tattttgctt tcctttatta tcgtttttcg
     5821 agaaagaaag gaaactgcgc aaacatgggc atggctgctc gttttgatgt tcattccagt
     5881 tgtaggattt attttatata tttttttagg aagagggatt tccaaagaga aaatctttga
     5941 tttgaagatc caagggaaaa tcggtaagaa tctagaaatc gaagaagata aacaagcatt
     6001 gatgcgaggg ctttatcctc atccgcctac tggacaagta gatgtgaaac aattgatcta
     6061 tatgcttact gtatttgaaa gtacgcttta tacgactaaa aatgaaatca ctttatttac
     6121 agatggtcgt gaaaaattcg atgcgctgat cgaggatatc aaacaggcag aagaccatat
     6181 ccatttccaa tattatattt accgcagtga tacattaggt gaagaagttc gagaggcatt
     6241 gacagaggca gcaagacgcg gagtaaaagt cagagtcctt cttgatgcat ggggttccac
     6301 acaagtttct tctagttttt ttcagaattt gaaaaaagca ggtggagaag tcgcattttt
     6361 ctttcctcta ttcgttccgt atatcaatcc tcgaatcaat tatcgcaatc atcgtaaaat
     6421 cgtagtgatt gatgggaaaa tcggatatac aggtgggttc aatgttggaa acgaatatct
     6481 aggattggta aagaaatttg gttattggcg agacaatcat ctgcgtatct atggagaagc
     6541 ggtttacacc ttgcaaaacc gttttttgat ggactggaat tctcagcata caaaagaagt
     6601 aggatattca ccggaatttt tcccgaccat tcattctaca ggtgatatgg ctgttcaaat
     6661 cgtgacaagc ggaccagata ccgaacatga gcaaatcaaa atgacatatt tgaaaatgat
     6721 ttcgttagcc aaacgggaga tattgatcca aacgccgtat tacataccgg atggatcgat
     6781 tcatgaggca ttgaagttgg ctcttctctc aggcgtaaaa gtccatatcc aaatacctaa
     6841 caaaccagat catcttctcg tttactgggc aacgtattct ttcgctgcag agctgatcga
     6901 gtatggggca agaatcgaaa catatgaaaa tggatttatc catgcaaaga cgatgattat
     6961 cgatggcggc atcgtttccg ttggttcagc taatatcgac gttcgctcat ttagattgga
     7021 ttttgaagtc aatacactga tctatgatga acgaatggct tctcgcgtaa gaaaagcatt
     7081 ctttgaagat tcaaagattt cgacgcatct cacaaaagaa gtgtatgaaa accgagggat
     7141 gatgatcaag atgaaagaag gattagcaag actgatttcg ccgctccttt aaaatacaaa
     7201 aaggacagga gagccatcaa tagatggaaa tctcgcgtcc ttttttctcg gggccatttc
     7261 tgagagtacg tcagaaatgg agaaagcgtc tcgcatgagc gaaattaact ttggggtatc
     7321 tcttgttttt tggggaaagc aagaaataag ctaatatatg ctattaaaag agacacattt
     7381 caaagccagt atagcataga aacttttaat gagatagctt tttttgcaat tatgagaatt
     7441 tattttgtta tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg atagaataaa
     7501 catactttag gattaaagga gatttctaat gaaaaaacta aaattattct taccaataat
     7561 aggattagct attcttttat caggctgttc aaagtggatt gatcgaggag aatccattac
     7621 tgcagttggc tcttctgcct tacagccttt agtagaaacg gttgctgaaa cctaccaggc
     7681 agaaaatcct ggaaaattca tcaatgtaca agggggcgga agcggaactg ggttgtctca
     7741 agtacagtca ggagcagtcg atatcggaaa ttctgatttg ttcgcggaag aaaaatcagg
     7801 gattgatgct tcggcattag tcgatcatcg agtagcagtt gttggaatca ctcctattgt
     7861 gaacaaggat attggcgtaa aagatatcag tatggaaaac ctgaaaaaat tatttacggg
     7921 cgagatcaaa aactggagtg aagtcggtgg gaaagatcag ccagtagtta tattgaaccg
     7981 tgcatcagga agcgggaccc gtagtacgtt tgaaaaatgg gtattggacg gagaaacagc
     8041 tgttcaggca caagaacaag attcaagcgg aatggtgcgc caaatcgttg cggacacacc
     8101 cggagcaatc agctatgttg ctttttctta cgtttccgat gatgtggata cgttgaacgt
     8161 taacggtgtg gaaccaacag atgaaaatgt gacaacgaac aaatggacga tttgggcata
     8221 tgagcatatg tacacaagag accatccaaa aaaactgaca aaagaatttt tagactatat
     8281 cttatctgat gaagtacaag ataatattgt cggagagcta ggctatatcc ctgtttcaca
     8341 tatgaaagtt gaacgtgatt ggcaaggaaa cattgttaaa gagtaatctt tacacaaaat
     8401 ttacaaactt agctaaactt ttacatgtta ttaaaaccaa attgatgttc aatttgtaca
     8461 ctgagtgagt atgttaaacc tgattaaggg ggagtcatct gtggaagatg tgaaaaaagt
     8521 attattaaca aaatcaaaac gttcaaaaat ggaacagcgc ggaaaattca ttagtttttt
     8581 gtgtatcgcg ctgatcgtgt tggttgtatt atctattttt tattttgtcg ctagtaaagg
     8641 attggccacc ttttttactg ataagatcaa tgtatttgat tttctgtttg gtacagattg
     8701 gaatccaagc caaataggag caaatgggaa acccatggta ggggcattgc ctatgatcac
     8761 aggttcattt attgtgacct ttttatctgc tattgttgcc acaccatttg ctattggagc
     8821 agcagtgttc atgacggaga tttctcctaa aaacggaacg aagatcttgc aaccggtcat
     8881 cgagctactt gtaggtattc cgtctgtcgt ttacggattt atcggtttat ctgttatcgt
     8941 gccttttgtc agaagtatct ttggcgggac agggttcggt atcttagctg gtacatttgt
     9001 gttattcgtt atgatcttac cgactgtcac aacgatgact gtggatgcat taaaggcagt
     9061 gccgcgacac tatcgggaag cttcattagc gctaggtgcc acaagatggc aaacaatcta
     9121 caaagtggtg ctgcgagcag cagtgcccgg aattttaaca gcagtcgttt ttggtatggc
     9181 tcgtgcgttt ggcgaagcct tagctatcca aatggttatt ggtaatgcgg cattaatgcc
     9241 aacaggactt actactcctg cttctacatt gacgtccatc ttgacaatgg gaatcggaaa
     9301 tacaatcatg ggaacagttg aaaataatgt gttatggtca ttagcgctga tcttgctatt
     9361 gatgtcatta ttctttaata tcgtgatccg tatgattggg aagaaaggag agctgaaata
     9421 atgaacgcaa aacgctcaga taaaattgct acaggtgttc tatatgtggt ttcagggatc
     9481 atcgtattga tcttagcagc tttattactt tatatcttag ttcgaggaat accgcacatt
     9541 tcatggtcat tcttgactga accttccaaa gcctaccaag aaggcggtgg gatcgggatt
     9601 caattattta actcgattta tttgcttttg attaccatga tcatcagttt tcctatctct
     9661 ttaggtgcgg gtatctacct ttcggaatat gccggaaaaa actggctgac agacgtgatc
     9721 cgtacgtcta tcgaaatctt gagctctttg ccttctgttg ttgtcggttt attcggtttc
     9781 ttggttttcg ttattcaatt ccagtatggt ttctcaatca tttctggtgc attagctttg
     9841 accgtattca acttgcctct tttgactcga acggtcgagg aatctttaca agcggtccat
     9901 tacacacaac gagaagctgg attggcgtta ggtttgtcac gctgggaaac agtgataaaa
     9961 gtggttgttc cagaagcgac accaggaatt ttgactggtg tgatcctaag ttcaggaaga
    10021 atttttggtg aagctgccgc attgatttat acagctggtc aaagcgcgcc agcattagat
    10081 tttacgaact ggaatccatt aagtgtttct agtccgatca gcattttccg ccaagctgaa
    10141 acactggctg tccatatttg gaaaatcaac acagaaggta cgatgcctga tggtgtttct
    10201 gtttcggctg gcgcttctgc cgtgttgatc attgctgttt tactgttcaa cttcggcgct
    10261 cgtgcgattg gcaaacgatt ataccgaaaa atgacttcag cctaaggaga agaagtaaaa
    10321 tgaaaaaata taattggaat gaaacaaatg tcattaccat gccaaaagat aaggaagttg
    10381 cattatatac gaatgatctg catgtttggt atggcgacaa tgaagcaatc aagggagtgg
    10441 atctagaatt cgaaaaaaat aagatcactg cattgattgg tccctctggc tgcggaaaat
    10501 ccacgtatct tcgttctttg aatcgtatga atgatgggat tgctaatacg aaaattactg
    10561 gaaagattat gtataaagac gtggatatca atgcgcctca agtggatgtt tatgaaatgc
    10621 gtaaacggat cggcatggtg ttccaacgcc ccaacccttt tagcaaatcg atttatgaca
    10681 atataacttt tgctttgaaa cagcatggtg aaaaaaacaa aaagaaatta gatgaaatcg
    10741 ttgaaacaag cttgaaacaa gcagctttgt gggatcaagt aaaagatagc ttggataaaa
    10801 gtgccttggc tttatctggc ggacaacagc aacgtttgtg tatcgctcgt gcaatcgcga
    10861 tgaaacccga tatcttgctg ttagacgagc cggcaagtgc cttggatcct atttcgacag
    10921 gaacggttga agaaacactt gtaaacttga aagaagacta cacgatcatt atcgtgacgc
    10981 ataatatgca acaagcagct cgtatcagcg actatactgc ctttttctat ctaggaaaag
    11041 taatggaata tgacaaaaca aacaagatat ttaccagacc aaaaattcag gcaaccgaag
    11101 attatgtgtc cggtcatttt ggttaagacg gaggcctcaa catgacaaaa gaaattatct
    11161 catccaaaga tcttcatctg tattatggaa agaaagaagc actaaaggga attgatctaa
    11221 gtttcaatca aggtgaaatc acagcgatga tcggtccttc tggttgcggg aaatctacct
    11281 atctacgttc gttgaatcga atgaacgatc tgattccaaa tgtaacgatc accggaagtg
    11341 ttttatataa agacaaagac atctatggtc cgaaaacaga tgttgttgaa ctaagaaaag
    11401 aaatcggcat ggtgttccaa cagccaaatc catttccttt ttctatttat gaaaatgtca
    11461 tctatggatt gaagttaaaa ggtgaaaaag ataaaaaagt ccttgatcaa gtagtggaag
    11521 aaagtctgaa ggcagcgtcc gtttgggagg atgtgaaaga taaactgcat aaaagtgcct
    11581 tgtctttatc cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg gcagtgaatc
    11641 ccgaaatcat cttattggac gaaccaacaa gtgcacttga ccctgtttct actgggaaaa
    11701 tcgaaagtat gctgttagaa ttaagagagc ggtatacgat gatcatcgtg acccataata
    11761 tgtctcaagc ttcacgtatt tcagataaga cggcattctt tttagatggt cacttgatcg
    11821 aatacaacga tacgaagaag atattcatga atccagaaaa acaagagaca gaagattata
    11881 tttcaggtcg atttggataa ggaggaaacg gaatgctacg tacacaattt gaagaagaat
    11941 tgctgaatct ccataatcag ttctacgaaa tggggatgat ggtgagcagc gctgtgcaca
    12001 agtcagttcg tgcatacatc aaacacgata aaatcttagc tcaagaagtt atcgaaaacg
    12061 atataaatat caataatatg gaaacgcgtt tggagaagaa aagttttgaa atgattgcat
    12121 tacaacagcc agttacaact gacttgcgta tgattatcac ggtcatgaaa gctagctctg
    12181 atttagaacg tatggccgat catgcggtgt ccgttgctaa atccacaatc cgcgtaaaag
    12241 gacagacgag aataccggaa atcgaaaaag agatctccga tatgtccgat tacgtgaaaa
    12301 aaatggtgga caacgtccta gttgcgtatg tcaaaacaga cgaaaaagat gcgcgtacaa
    12361 tcgccaatat ggatcaaaga gtcaatgact atttcaatcg aatctataat gcaaccatca
    12421 aaaacatgca agaaaatcct gaaacagtca tcagtggcac agactatttg aatgttgcca
    12481 gctatctaga acgaatcgga gactatgtga caaacatctg tgaatggatc gtctacttgg
    12541 ctacagggaa aatcactgaa ttaaatacga accacaatga gattttctaa actgaagttg
    12601 tgattctgct gtttatttag gaaaagaggt tgtgaaatca gcgttttgtc ctgagtatta
    12661 gagaaacaaa cggtaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaagg
    12721 tcagctgatt gaacaccgtt tattctggaa aagggtttgt gacaaaacat gtcacaaacc
    12781 cttttcatta ttccaataga aaggataaag aatgcaaaaa aagattagca tcatcatcac
    12841 tctatttttt atttgtatac ttgcttcatg gtttattcag cgtcaagctg ctctatatga
    12901 cgtacctgtc atcgagatcg aaagagtgag aatagaagag gatcaacaat tgattacagg
    12961 aagattgatg aatacggacc aaaaaggaga agtgatttct ttaacggctc cttatcaaaa
    13021 aaatcaaatc gaaacagcaa atataacaac aagacaaatc tatcttgtcc aaatgcatgg
    13081 agatgaatgg caactagtag caaaaaaaaa tgatggctgg ctttttttct ttacaagtgt
    13141 cttcattggc accatgttgc ttattggtgg aaaacagggc gtatttgctt taggaagtct
    13201 catcatcaac atcgttttac ttttactttt gttgctcctc tttaccaaaa cagaaggtat
    13261 ttctctctta aatatagcaa tcttatttgt cgttataggg ataatcgtta gtatattggc
    13321 gttagacgga tggaatagaa gcagtataat caaaatttcg gctgcagtta caagtgtttt
    13381 tttagcattt tttatttgtc ttttgacaat gaatcattgg aaagatcaag gactgcgcta
    13441 tgaagaactg aattatctga cacgaccgta tcgttctgtt tttctttcca gcgtgctgat
    13501 tgggacagct ggagggacac tcgataccgt gatcacagtc attgcgacat tagaagaatt
    13561 gaccaagcaa caaccgaaaa tatcagcgaa acagttatgg ctttcaggaa ggcaagtggg
    13621 ccgagatgtg attggttcga tggctaatat ccttttattc gtctatatca gcggagcgat
    13681 tccatcgctt ctgttatatt taggaaatca atggtctttc aaagaaacgg tagaacgaca
    13741 tttatcatta gaacttttac gcgtcattgc tggcagctta ggaatagtat tatcaatccc
    13801 catcgcggtc ctcttttttc tcgctgtaag gaggttaaaa aaatgaatgt acttattata
    13861 cttgttggga tttttgcaat cagtgtgcta tttgtcggtg gtacgcaagg tatgtatatt
    13921 cttttaggat tattcattaa tttaggaatc tttttcctat tattattcgg ctatcatcag
    13981 aagtggccga tattggtatt gtcgattata gggttcctat tgatcgctgt ggttatctta
    14041 ttttttatca atggttataa tctgaaaatg cgagccgctt ttgccagtat actgatcttt
    14101 ttgttttgtt tcctactatt gattcctatc actgattttt tagcgatcca aggctttact
    14161 tcgatagaac tagaagaact atctggctta gataagacgt tagcaattga ttttagacta
    14221 cttacgaggt cgttgcttct gatcagtctt agcggagctg ttttagatgc gagtgtagcg
    14281 atttctagtg gaacattcga agtatatcag gcaaatccgc aaatgtcttt taaccaattg
    14341 cgccatgcta gctttgccat tgctaaaaaa atattggcat caacagtgat gacgcttctt
    14401 tttgcgttta tggggagttc tttagcgttg attctttggt ttattgactt agacatttct
    14461 tttcagcaaa taatcaatga aaaatcattt gtcttggaat atacgatggc tattcttacc
    14521 acactggccg cactacttgt cctgccctta acttgcgtga ctacagcata tctctttaca
    14581 aaaaagaaag aacatctcaa gatggagtaa aagttacaac ttaagtccta tgacattttt
    14641 actgaatcag tgcataatat ggatgtaagg aaaagcttgc aaacaatttt gcttaaggag
    14701 gcaatttcca tgaaagaaag agaacgtatt ttagacttag tgaaaaaagg aatcctttcg
    14761 actgaagaag gcttagattt attagaaagc atggcaactg aaaaagacga aaaacaaatc
    14821 aaaaaagaag cagacaaggt aactgctagt cataaagaaa aagatcaggc aagtcaattg
    14881 atcgatgaac ttgaaaacgg tgaagaaaaa atttctgagc cagttgatcc aaaagaaaaa
    14941 gaaagacaag atcaagaaaa cttagaaaag atcttagatg aactagcaac tgaagcgaat
    15001 aaaacttcgg cgcgtctaga cgaagtaaat gctcaattcg ctgacaataa ggcagcacgt
    15061 aatgagaaac aggaagcatt gatgcagctg aatacaaaag aagaattagg cgagttgaca
    15121 gaagaagaat tagcacagcg tcaaacgtta gaagcagaaa tcaaagagtt agaagcaacc
    15181 ggcgatacac tattagaaga acaattgaag ctggaagcag aattaaaaga tatccgcaag
    15241 aatcaatggt ctgagaaaaa agaaacattt acggataagt ttgaattgcc agatgattgg
    15301 aaagatcaag cgacagatac gctgaatcaa gtaggcgaaa aaatgtcaga agctggatca
    15361 cagttaggca aattcttgaa gaaaacattc caaactgttt cagaaacagt aaatgataac
    15421 atggaatgga aagatgtcag cttacgcgtc cctggaattg cgacaacgaa atttgaacac
    15481 gaattttatt atgaagctcc tgcagcaagt atccttgata tcaaggcagc aaacggaaac
    15541 gtgacgttaa aaacgtggga cagtgatgat gtgaaagtgg aagccaagat caaactttac
    15601 ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac ggagccagat cgaggtcaat
    15661 gaagatcata tctccttcca aattccaaat aagcgtgtgc gtgcagacct tgttttctac
    15721 ttgcctaaac gagtatacga ccatgcagct atcaaacttc taaatggcaa catcatgatt
    15781 gagacattgg aagctaaaga tatttataca aaatctacga atgggaacat catcgtgaac
    15841 caattaactg caacgatgct ggaagtagaa ggtgtaaacg gcaatatcga tattcgaaac
    15901 gggaacatct tagattctat tatagaaaca gtaaacggta cagtgacatt tggtgccaca
    15961 ccagaaaacc tttctgtgtc tttagtaaat ggtgatgtac gtcttacgat taaagaagat
    16021 aatttgaaaa aagtagaagc aagttcagtg aacgggaatg tcaaagttgc tttaccagat
    16081 agcatcggtt tggaaggaca cgctaaaaca agtcttggaa gcattaacag ccgtctgtcc
    16141 aattacgaag tagttcgtga gaaaaaagaa cggacaaacc aaatgttgca attccgtcgt
    16201 gtttcagatg atgagatcgc acaagtgcag ctttcaacga caacaggaag tatctattta
    16261 aaagatacag ataaataaag aggttgtgaa agagatgttc agccttgagc aataagaagc
    16321 gattttttaa aatagcttcc cctattttaa aaaaatgtgc cttattgccg ataggctatc
    16381 tcttgaacac cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga
    16441 acagaggtga gacatgatga aaaaaaagtt gacaaaatcc aataaaaatg ttgttttgac
    16501 aggtacttta gcagggattg gggaatatat cgggattgat cccactgtta tccgtgtgct
    16561 ttatgtattc ttaagcttag ttgcttttgg aagtccaatc attttataca ttttattagc
    16621 tttgattatc ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta
    16681 tgaaaaaaac aattatcgtg aacaaacaaa aaaacgcaaa gaagcggaaa aaatcgatga
    16741 tgacgaatgg agtgactttt aatgtcgtac tttcagcgta ttttggttaa tacgttaaca
    16801 ttcatatcgt tggctgttct ttttcctgcg atggtccatg tgagcagtat ttggacggcc
    16861 gtccttgcca gcttcgtatt atctgtgttg aacatgtttg tcaaaccgat tttgacgatc
    16921 ctgtccctgc cctttacatt agtcacattg ggattgttta cgtttgttat caatgcagcg
    16981 attcttaaaa tgacttcctt ttttgtagga gcagaacatt ttagtttttc ttcatttggt
    17041 gcagcgttat tcgtagctat cttgatgtca cttgtcaatg taattgttag tgaacataac
    17101 agcagaaaat aaaaaaagct gtgccgagat ctcggcgcag cttttttgtt tagaataaag
    17161 aatgcgtttt ctattacatt ggtaattaaa taaaaacgtt ttttttaatt ggtagtactt
    17221 ttggctagac aatgataaaa tgatagagaa cactctaata gaaatgaggt agcatgaatg
    17281 tcagaagttg taaaagttag ccaattagta aaaaaattat ctttggaaat cgtcacaggc
    17341 gacgagaaaa gtttagaccg cgtcatcgtg actggggaca tctcacgccc aggactagag
    17401 ttgaccggtt attttaatta ttattcccat aatcgtttac agctatttgg cagtaaagaa
    17461 atcagttttg cggaacggat gatcccagaa gagcgtttga tcattatgag aagattatgt
    17521 agtgaagata tgcccgcatt tattatttca agaggattgc cggctccgga agaaatgatc
    17581 caagcagcca atgaaaatgg gatagctgtg ctgcgttcgc ctatctcaac gtcccggctg
    17641 ctaggtgaat tatctagtta tttagatgga cgcttagccc ctcgaacaag tgtccacggt
    17701 gtgttagtcg atgtctacgg attaggtgta ctaattcaag gggacagtgg gatagggaaa
    17761 agtgaaacgg cgttggaact gattaagcgc ggacatcgat tgatcgcaga cgatcgagta
    17821 gatgtctatc agcaagatga actgacggta atcggagaac ctcctaaaat cctggaacat
    17881 ctgattgaga ttcgagggat cggtatcata gatgtaatga atcttttcgg tgcgagtgcg
    17941 gtacgaggct ctatgcaagt ccagttagct gtttatctag aagcatgggc gaaagataag
    18001 aaatacgacc gtctgggctc tgaagacacg accgttgaaa tcgcggaagt gggcattcca
    18061 caaataaaaa tccctgtcaa aacaggacga aacgttgcaa tcatcattga agtagccgca
    18121 atgaatttcc gcgcacgtac aatgggcttt gacgcaacga aaacatttga agaaagatta
    18181 agtcgcttga ttgaagaaaa ctcaggcaat gactagcaag gagtgacaat atgttagcac
    18241 aaatcaatcg agttgctttt gagcttgtag gaattcctat ctactggtat gcattgatta
    18301 tcgtatcagg aatcattatt gccatgtggc tcagcagcag agaagctgtt cgggtaggat
    18361 taaaagcaga tgacgtgaca gattttatgt tgtggggatt gccgatttcc attattggtg
    18421 cccgcttgta ttatgtttta ttcgatcttc cccaatatat agctgatcca atccagatat
    18481 tcaatatccg ttcaggagga ttagcgattt acgggggatt gattgcaggt gcaatcacac
    18541 tgtacttttt taccaaatat cattttattt ctttgtggac ctttttagat atcgctgcac
    18601 caagcgtatt attggcgcag gcaatcggac gatggggaaa tttcatgaac cacgaagcgt
    18661 atggtccaga cacgacacga agtttcttag aaagtctgca tttaccgcaa ttcatcattg
    18721 ataacatgta tatcgaagga atctatcgac aaccaacctt tctttatgaa tcactttgga
    18781 gtttgattgg atttattctg cttttggttt tgcggaagaa accacatctt ttgaaaaaag
    18841 gagaagtcct tttatgttat ctaatgtggt atagtttcgg acgtttcttt atcgaagggt
    18901 tacgcacaga cagcctttac tttttaggtg tgatccgcat ttctcaatta ttgtctgctg
    18961 ttttatttgt cggcacattg attctgttta tttggcgtag aaaaaaagga agtttaccgg
    19021 attatgaccg ctcattagga aaaaatcaac aaattatttg attttcagag cgttaaaaat
    19081 gaaaaaaatt tcaggagtgg taaaatgaat tatagagtag cggttttagg accaggctct
    19141 tgggggactg ctttagcaca agtcctcgct gaaaacggac atgacgttcg gatttgggga
    19201 catcgtgctt cacaagtaga agaaatcaac acccaacaca ccaatcaacg ttatttacca
    19261 gatcatcatc tacccgattc catccgagca ttcaccgata tggaagatgc agtaaaagat
    19321 gcagatgcgg tactattcgt tatcccgacg aaagcgatac gttctgttgc aaaagaacta
    19381 gttgccaaat tgaatacgaa gccagtgatc atccacgcaa gtaagggatt agaacaggga
    19441 acgcataaaa gaatctctga aatcttgtta gaggaaatcc cgacagaaaa aagaaaagct
    19501 gttgctgttc tttctgggcc aagccatgcg gaagaagtag caaagcatga cattacgacg
    19561 atcacagcgg caagcacaaa tgtaaaagca gcaaaatatg tacaagagct gttcatgaac
    19621 cattattttc gaatctatac caaccctgat gtgattggtg tagagatggg agcagctttg
    19681 aaaaatatta ttgccattgg agctggtgct attcacggct tagggtttgg cgataatgca
    19741 aaagcggcaa tcatgacgcg cggattagct gagatcagcc gtttaggtgt agcaatgggt
    19801 gccaacccgc tgacctttat tggcttaagc ggagtaggtg atctagtcgt tacttgcaca
    19861 agtgttcatt ccagaaactg gcgcgcaggc aacttgttag gacaaggtca gcctctagaa
    19921 gaggtgctag ccaatatggg aatggttgtt gaaggtgtga atactactca agcagcgatg
    19981 gaactagccc aaatgatgga aatcgaaatg cccatcactc aaacaatcta tgaagtgctg
    20041 tatgaacaaa aagaaatcaa aacagccgca aaagaaatca tgttacgtga tgggaaaaaa
    20101 gaaaatgaat tctcattaac aattttttga aggagcgaat gtatatgaaa gtaaaaaaag
    20161 cagttattcc agcagcagga ttaggaacaa gatttttacc agcaacaaag gcgatggcaa
    20221 aggaaatgtt gccaatcgtg gacaaaccaa cgatccaatt catcgttgaa gaagcgttga
    20281 aatcaggaat cgaagacatc ttgatcgtta caggaaaagc taaacgacca atcgaagatc
    20341 actttgattc aaatatcgaa ttagaaacaa acctaagtga aaaaggaaaa accgaattat
    20401 tgaaattagt agaagaaacg acagatgtca atttgcattt catccgtcaa tctcatccaa
    20461 aggggttagg acacgccgtt cttcaagcaa gagcctttat tggaaatgaa ccgtttgtcg
    20521 ttatgctagg cgatgatatc atggaagacg aagtaccatt gacacagcaa ttgatcgatg
    20581 actatgagcg tacacacgct tctacgattg ccgttatgca agttcctcac gatgaaactt
    20641 caaaatacgg aatcatcaac ccaggggaag tactggaaga tgggttatac aatgtcaaaa
    20701 actttgttga aaaaccagat ccaagcaaag caccaagtga tttagctatt attgggcgtt
    20761 acttattgac tccagaaatc tttgatgtat tatcagaaca acaaccaggc gctggaaatg
    20821 agatccaact aactgacgca atcgatacgt tgaataaaac acaacgcgta tttgcccgta
    20881 aattcacagg taaacgttat gacgttggtg acaaatttgg ttttatgaaa acaagtattg
    20941 aatatggtct gacacatcca gaagtagggg cacctttgcg tgaatacatc atgtctttag
    21001 gtaaaacatt agaagcagaa ggaagcaaga tcaatcataa tccaaaagga ataacagaaa
    21061 tcaaagaaga aaagaaaaaa taatcttttt ttctaacgag cttcagctgc taattgaata
    21121 aaaagtagtt gaagctcgtt tttatttgtt taaaaaacac gaaaatgcta tccttataga
    21181 aaagagagac aagaagggag tttgatcatg tcaggaggag aaattgcagg attgatcgca
    21241 gcaatcgcct ttgcgatact tgtcgttttc atcgttcgcg tattgttgca agtttccaaa
    21301 actttagaaa aagtagacca gactgtcgca gaagcaaata cgacaattga gatcgtcaca
    21361 caagacgtag atcttttatc tagacaagta gaaggcttgc tagtcaaaag taatgagctg
    21421 ctcttggata tcaatggaaa agtcgcaacg atcgatccat tgttcacagc agttgctgat
    21481 ttaagtgaaa gtgtatcgga acttaatcat tctagtaaaa acatcgtgac gaaagtcggc
    21541 ggaatcggaa aaacgacagc taaagcgact gttgccggaa aagtcggtgg aacagcgatg
    21601 aaatttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataagaa tggctaaaaa
    21661 aggtggattt ttattaggtg cagtagttgg aggtacagcc gcagcaatcg ccgcattatt
    21721 gttagcaccc aaatctggta aagaacttcg ggatgaattg gctgctcaag cagatgattt
    21781 gatggacatg gcttcagatt acacagatat ggcaaaagaa aaatcatctg agttaacagg
    21841 tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggggatctag caggcaatgt
    21901 taagcagaag gtaagtgaat cagcagagga agcaggaagc gaatcagatg atatcttgtt
    21961 aaacctcaaa gaacaatcag ctgatttatc taatcgtttc aaaaaaacag cagaagatct
    22021 gaaaaagcca gcagacgaat taggtagtat tgctaaagat acttcagaag atatcatgat
    22081 cgatgtaaaa gatgctgcag ataaagcagg agagacggtc aatgaaggaa tgtcagaagc
    22141 aaaaccagtt gcagatgaca caaaagaaat gttgaaaatg gcaaaagaag atgcaaaaga
    22201 agatgcaaaa gaagctaaaa aagaaatgaa agatacaatg acagattcga ctaccaatca
    22261 ctaagattac tcatttctag aataaaaaag gtgagagcgg accagctctc acctttttta
    22321 ttttcattac tctaaaacaa gtaattcttt tggtgtagtc gtgaatggca cacaaccatc
    22381 acttgtcaca tacacacaat cttcgatacg tacccctact ttttcaggaa tatagattcc
    22441 tggttcaaga gagaagcaca tcccttcttc aatcactaag tcgtttcctt ctacaagaga
    22501 agggaattcg tggacggttg ttccgattcc gtgacctaaa cggtgattga aatattcgcc
    22561 gtagccatag ctgttgatca cgtttcgtgc gatttgatca agttcacctg ctgtcacacc
    22621 tgggcgaact gcctcttgtg cggcaagctg tgcttcaaga gtgatgttat agattttttc
    22681 ttggaattca gttggttttt gataagcaac tgtacgagta gcatcactgc aatagccatt
    22741 ccaaataacg ccaagatcga ataacactag ttcatttggt gaaaccttcg tgcttcctgg
    22801 tgtaccatga gggcttgcag catttgctcc tgcaaggact aatgtgtcga aagacatttg
    22861 tgaaacacct tgttttttta attggtattc gatttctgcg ataattgctt gttcagttac
    22921 gccttctttt actgttttga agccaatttc aaaggcaaca tcggcccatt taccagcttc
    22981 gatcagtttg tctttttctt ctaccgtttt gatcagttgc agacgctgga taatagaagt
    23041 tagatcggtg taagaatctg tacctgtcaa gatatcagag atagcatcga agcgatcgac
    23101 agtcagagca ttcttttcaa tacctacatt accaggttgc ccatagcgtg cgcgaagctc
    23161 ttttccgatg atctcccaag gattttcgct gtctaagtag ctacgtacat cgtacgccca
    23221 gctgctgttt tgagcatctt caacttccag agcaggagtg aaaaggaaag gatcttgttc
    23281 cagagggata aaaagagcta gtacacgctc gtgaggatca ctttcatatc cggaaaagta
    23341 agcgatgtgg ccagggtcgc tgatataagc caattctaat tggtgttgtg ccatccattg
    23401 acgtagttca ttgattttta cgttattcat ttggcaacct actttctaaa ttaattttgt
    23461 attcaataat tgtaccactg attgattaaa agaccaacgt tcttaaaact gaaaaaagta
    23521 aaaattttca tgaaaacaga gataaaacgg ttgcaattgt tgagattatt tgatatccta
    23581 ttttagaaat gtaagacgta ttttcagaaa acatataaaa tatgaatagg agaacacaaa
    23641 tggaaaagca aacaatcact atctatgatg tagcgagaga ggcaaatgtt tcgatggcta
    23701 ctgtttcacg tgtcgtaaat ggcaatccaa acgtcaaacc agcaacaaga aaaaaagtct
    23761 tggaagttat cgatcgcttg gattatcgtc caaatgcagt cgcacgtggc ttggcaagta
    23821 agaaaacaac aacagttggg gtaatcattc ctgatgtcag caatatgttc tttgcctctt
    23881 tagcacgtgg tatcgacgat gtggcaacaa tgtacaaata caatatcatc ttagcaaact
    23941 cagatggcaa cgatcaaaaa gaagtcaatg tattgaataa tttattggct aagcaagtgg
    24001 acggcgtgat ctttatgggt caccatatca cagacgaaat ccgtggagaa ttttcacgtt
    24061 caaaaacgcc tgttgtacta gctggttcca ttgatccaga tgaacaagta ggtagcgtaa
    24121 acattgatta tgtatcagca acaaaagatg cagtaaataa attagcaaaa agcggcaata
    24181 aaaaaatcgc ttttgtcagc ggtgcgctga ttgatcctat caatggtcaa aaccgtctaa
    24241 aaggctataa agaagcatta agcgaaaaca atctaccata tagcgaagga ttgatttttg
    24301 aggcacaata caactttaaa gatggtctgt ctctagttga ccggatccat aatagcggtg
    24361 caacagctgt ctatgtttca gacgatgaat tagccattgg tatcttggat ggtttattag
    24421 accgcggcgt aaaagtacca gaagaattcg aaatcatcac aagcaacaat tctttactga
    24481 cagaagtagc tcgtcctcgt ttgacaagta tcacacaacc attatacgat ttaggagcgg
    24541 tatccatgcg cctattgacg aaaatgatga ataaagaaga agtcgaagaa aaaacgatta
    24601 tcttgcctta tggaatcgaa gaaaaaggat caactaaata ataaaagatc aatcatttaa
    24661 aggttgttcc caaagtggtc aacttctgga acaaccttta tacgagaaaa ggggttatga
    24721 caaaattttg tcataacccc tttttttgtg actttaaaaa aaagagagaa tggcggaaac
    24781 aaaccttacg tccatcgtat acgacacaat ctgtgcgtag atgctacatt ttgttcacta
    24841 ctatcaaaat tactttaaaa tgaaaacgtg ataaaaaaga aagtagaaaa gtagaaaagt
    24901 aactctaaaa aatccaaaaa aatccaggct cgattgcgta cttatcttaa aggagaaaca
    24961 atccatcata cataacccga aaggagactt ctatttgcct aagaaaagac gaaagaccat
    25021 agtcatcatt ttgaccattc ttactatagg aatcggagga aaattttaca tggacaaaag
    25081 agaagcgcaa aaacagcaag atttactcgc agtagaaaaa cagtccgtca aagttctgaa
    25141 aaataccttt gcggatatca aagaagtgaa aatcgaacaa tttgagaaaa acgacatgac
    25201 aggaatctat gaagcattga taacgatgac caatacagaa ggaaaagccg tttattttga
    25261 ttatagcttt acaaaagaaa gcaatgagat agatggctat ggtgttgaaa atgaagaagt
    25321 acaaaaagaa gggattacgg ttgatgagat tatagttatc tatacagatg ggagtgagga
    25381 aaaagtatga caattactga taagcagtat aaaggaataa gtaataaagt atattggcta
    25441 gatccaaaac atcctcaata tagtaaaagt tacaaagaag gcgccattaa aaactttgga
    25501 gaaacaaaat tccaaatcct aaaaatcaaa aactccttcg acggcatgca ggcgatggct
    25561 gtagcgccaa tcaagaacgg taaagtggat atttctcaag tagtgattgc ttttgcagga
    25621 acgaacttaa gcgattggaa agatttggaa acggatgcaa gaagtgttat gaatctctat
    25681 gggaatagac caggagcaag tttttctgaa aaagcaacaa caaacaagat tttatccgtt
    25741 agtggattga aaacagcggg gcaaatcaat tcagcgattg catttgctaa tgaagtaaaa
    25801 aaggaatatg gagaagctgc tatttctact accggccatt cattaggtgg attcttagca
    25861 ttatatgtag cagcagagaa acaatggaaa aatgtcggct tcaacgggcc ggacccgtat
    25921 gatctcttat caccagaagc aaaaaaatgg gtggaagaaa atcccggtat gctcaccaat
    25981 taccgaaatc acgccgattt tatcggtaat tttggtggta atggcacagg tgctgaagtt
    26041 cggatcagta tggaaacagc atacttgaat ctgcttgctt cccacagttt aacctcttgg
    26101 aagtttgatg aaaatggaaa gctgattatt cctgaaaatg aaaacaatgt taaagctcga
    26161 aaacaacaga aagaaaacca agcgacccag ttgttttcct atcagttgca agaactgaac
    26221 aacatacgta aaaaactacg attaagtggc ggccggctga cagttaatga acaaatttac
    26281 ttagatgaca cacaagctag gttagtggtg gaaaagttat cggtaaaaat gaaaagcgct
    26341 atggaagaag ttatccgcat caaccaaaga ggaatttttg agctagagaa aaaatggcaa
    26401 gaaggcttgc ggcaagcacg tgttgtcgcc ccttcgttga ctgaacagga aatcatagaa
    26461 gcattagcta gtgtgggcgc cacaaaagag aacttagtcg attctgcgaa agaagtctat
    26521 ctgctacgaa tcaataaggc aagacgtatg ggggaggcct ttgatacatt ggcgaaggag
    26581 atccaagaca aaatcaatga aatcgtggca cgagatcgag aattagcaca gcaattcaat
    26641 taaaggagaa gtagatgaaa ctagaagaat tggaaagaga ttatcgaagt gaacgaagac
    26701 aattagaagc tcaagaagat gaattgatcc actttaagaa tcaaggatta cgattgatgg
    26761 agcaagagga gcagcgactg catcatgtat taaagaactt agctttagat aatgagcctc
    26821 tgtttcaagc aagacgaagt tattcgaatt atcaggagca ttatcaacaa gccctacaag
    26881 tagaacgacg aaaactctat cagcagcaag acgaactaga acgttcctat cgtcaaaaaa
    26941 tcagacaaat aactatggag aaatcatctt atgaatgaat ttttaatgca attactaaaa
    27001 gaattagaga aaaatctaca tcgatcaaat gtacgttcag ttggtatggg gatatctcag
    27061 caaacttggg cagaaaaacg attgacagat caaaaccacc aaatgactgc agaagaagct
    27121 actcaagaat tttctaaccg acatgcacac ttaaaagaat cagtaaaagg gcaatttgca
    27181 gaaaaactaa ccgaattatt ccaaagacaa gaaaagaaat taaaacagtt tcgttagtaa
    27241 agttatatag a
//
LOCUS       DAZKNO010000018        26075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000018 DAZKNO010000000
VERSION     DAZKNO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.018"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..423)
                     /locus_tag="ACG8MY_000564"
     CDS             complement(<1..423)
                     /locus_tag="ACG8MY_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429793.1"
                     /translation="MKKVYQKCLWISYITVGIVGFFFIVAVTLGTIFLWFTNEDLLLK
                     GLMSVISCVLLILLGTEINKLLPSRWQEKKKEMREEIEIKSTDWIKHTWLPLKRNSKR
                     KEFWILQLWPLGKLCSYLARFILDLIIYSIVVFGTAYYL"
     gene            complement(441..671)
                     /locus_tag="ACG8MY_000565"
     CDS             complement(441..671)
                     /locus_tag="ACG8MY_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429794.1"
                     /translation="MKRANLVGKDTFAEQKVLIKAYLKVVKAKEICQEVNLSNTTISE
                     LKNGRKSLDTLKFRSFEALYLAAKKYFTAERK"
     gene            complement(694..1500)
                     /locus_tag="ACG8MY_000566"
     CDS             complement(694..1500)
                     /locus_tag="ACG8MY_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429795.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEISTNAFVNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKENI"
     gene            complement(1511..2155)
                     /locus_tag="ACG8MY_000567"
     CDS             complement(1511..2155)
                     /locus_tag="ACG8MY_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429796.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNLVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            complement(2171..3376)
                     /locus_tag="ACG8MY_000568"
     CDS             complement(2171..3376)
                     /locus_tag="ACG8MY_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0429797.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKNSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            complement(3376..5400)
                     /locus_tag="ACG8MY_000569"
     CDS             complement(3376..5400)
                     /locus_tag="ACG8MY_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFQ0429798.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            complement(5563..6156)
                     /locus_tag="ACG8MY_000570"
     CDS             complement(5563..6156)
                     /locus_tag="ACG8MY_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429799.1"
                     /translation="MRVKGQNSVDRKSILQFGKPEERSILDLFPYKSCTEEGVLITKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(6157..6489)
                     /locus_tag="ACG8MY_000571"
     CDS             complement(6157..6489)
                     /locus_tag="ACG8MY_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429800.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTIGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(6580..7053)
                     /locus_tag="ACG8MY_000572"
     CDS             complement(6580..7053)
                     /locus_tag="ACG8MY_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429801.1"
                     /translation="MEDILIATFITALGAFLTIFVATIKGFKDTESLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTELFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENLNRLNDEMQRVHLENEELRAQNYELIQMKRNLEQKIFELKEEN"
     gene            complement(7114..9474)
                     /locus_tag="ACG8MY_000573"
     CDS             complement(7114..9474)
                     /locus_tag="ACG8MY_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0429802.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKIIGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNVAKNVGSGAKNLASAGKSGLSNLNDIRKNGFGN
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSETLEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSEEGQNGQLPKTDPEQYRYKSNINS
                     ENEKRISDLPKRNQEVQTDDDSIDLPNDNGVEVGTEKSTKNGLPKAQNLPMKDSRLNQ
                     FKENDQSFVHQSTAGNVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAP
                     EPIQKVHTGYKPANVAFTAPTKINVTRDTKNRNNALKEQLLKDIKP"
     gene            complement(9471..12080)
                     /locus_tag="ACG8MY_000574"
     CDS             complement(9471..12080)
                     /locus_tag="ACG8MY_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0429803.1"
                     /translation="MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYQDPDNEFKQLNALDEFMNQLPPGNTAKKQYGATKIGSDKAKGNIL
                     STAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHQGKK
                     FHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYVAWYEFSQRVLKDEEGNIV
                     YRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHII
                     VSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLW
                     DLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENST
                     INSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHG
                     ETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDIYARRLYHNMH
                     DQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQEYLEKLRQNEEITEVIR
                     KRLLLEAEKAVKE"
     gene            complement(12080..14143)
                     /locus_tag="ACG8MY_000575"
     CDS             complement(12080..14143)
                     /locus_tag="ACG8MY_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429804.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGQLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            complement(14213..14515)
                     /locus_tag="ACG8MY_000576"
     CDS             complement(14213..14515)
                     /locus_tag="ACG8MY_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429805.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            complement(14533..14775)
                     /locus_tag="ACG8MY_000577"
     CDS             complement(14533..14775)
                     /locus_tag="ACG8MY_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0429806.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            complement(14772..15236)
                     /locus_tag="ACG8MY_000578"
     CDS             complement(14772..15236)
                     /locus_tag="ACG8MY_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429807.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            complement(15263..17353)
                     /locus_tag="ACG8MY_000579"
     CDS             complement(15263..17353)
                     /locus_tag="ACG8MY_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0429808.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKTTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            complement(17350..17610)
                     /locus_tag="ACG8MY_000580"
     CDS             complement(17350..17610)
                     /locus_tag="ACG8MY_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429809.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            complement(17623..18384)
                     /locus_tag="ACG8MY_000581"
     CDS             complement(17623..18384)
                     /locus_tag="ACG8MY_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0429810.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLFSDQLKLVSNGKTKTIDQQT
                     GVFEFSIEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            complement(18400..19152)
                     /locus_tag="ACG8MY_000582"
     CDS             complement(18400..19152)
                     /locus_tag="ACG8MY_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0429811.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIVAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWTWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            complement(19167..21143)
                     /locus_tag="ACG8MY_000583"
     CDS             complement(19167..21143)
                     /locus_tag="ACG8MY_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0429812.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGAGGITVNGVKSETSPYSVFKWGDIA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGIGIKATPLEGAEFAAKNAEGKYYGGLVDTDKNGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            complement(21196..21866)
                     /locus_tag="ACG8MY_000584"
                     /pseudo
     CDS             complement(21196..21866)
                     /locus_tag="ACG8MY_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
     gene            complement(21948..22964)
                     /locus_tag="ACG8MY_000585"
     CDS             complement(21948..22964)
                     /locus_tag="ACG8MY_000585"
                     /inference="COORDINATES: protein motif:HMM:NF037933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0429813.1"
                     /translation="MTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRVDT
                     FYGDLIVPTIIAIILIVKWTEFTVFDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIW
                     NPSNKLSDIWINTNTIGSSLMTLALLITGFASSFVKWYMRLLSLPALILAGATIWVCQ
                     SKAALYALVIFVILEILPKKIFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGR
                     EEIWRKFYETLGEKTGQIFVGMKPFFFHIGNQILGNHNTYHALVNHYGLIGFALSIIP
                     LLSFLWLLVRKCELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQY
                     KTTR"
     gene            complement(23116..23805)
                     /locus_tag="ACG8MY_000586"
     CDS             complement(23116..23805)
                     /locus_tag="ACG8MY_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HFQ0429814.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGHQKDKIAEKVDGVTK
                     TIHIQEQDKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLDFEANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(23932..24170)
                     /locus_tag="ACG8MY_000587"
                     /pseudo
     CDS             complement(23932..24170)
                     /locus_tag="ACG8MY_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24236..24475)
                     /locus_tag="ACG8MY_000588"
     CDS             complement(24236..24475)
                     /locus_tag="ACG8MY_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429815.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARSYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWRIYNGIKDLKPARPVVKRKKKITQE"
     gene            complement(24501..24623)
                     /locus_tag="ACG8MY_000589"
     CDS             complement(24501..24623)
                     /locus_tag="ACG8MY_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429816.1"
                     /translation="MKFFEVKSPCYYALIAENNEEECAEITTNNKKLVLSLDIE"
     gene            complement(24642..24983)
                     /locus_tag="ACG8MY_000590"
                     /pseudo
     CDS             complement(24642..24983)
                     /locus_tag="ACG8MY_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            complement(25602..25934)
                     /gene="traE"
                     /locus_tag="ACG8MY_000591"
     CDS             complement(25602..25934)
                     /gene="traE"
                     /locus_tag="ACG8MY_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0429817.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT         7213 a         5323 c         4038 g         9501 t
ORIGIN      
        1 taagtaataa gccgttccaa atactacaat cgagtaaatg attaaatcaa gtataaaacg
       61 agccaaatag ctacaaagtt tacctaatgg ccataattgt agaatccaaa attctttacg
      121 tttgctattt cttttcaaag gaagccaagt gtgtttgatc cagtcggtcg attttatttc
      181 aatttcttct ctcatttctt tcttcttttc ttgccaacga gaaggcagaa gtttgttgat
      241 ttctgtcccc aacaaaatca ataggacgca tgagattaca ctcatgagtc ccttcaataa
      301 taaatcttca ttagtaaacc atagaaaaat cgttcccaag gtcacggcta caataaaaaa
      361 gaagccaacg atacctacgg tgatatagct gatccataaa catttttgat acactttttt
      421 cacataaaat cctcctgttt ttattttctt tcagccgtaa aatatttttt agcagctaaa
      481 tacaaagctt caaatgatct gaattttaag gtatctaaac tctttctacc attcttcaac
      541 tcagatattg ttgtgttaga taaattcact tcttgacata tttctttagc ttttacaact
      601 ttgagatagg ctttaatcaa tactttctgc tcagcgaaag tatctttgcc gaccaagttc
      661 gctcgcttca tttcttttac tccacctatt cttctaaata ttttcttttg tcatacgatc
      721 aattttttct tgaagttttt gaatgatctc ttcttgatct ttcatttgcg ttttcgatgt
      781 ttcaatttct tgctggtaac tattaatctt attttcagta ttttcctgat cgtctgcaac
      841 ttgataaggc aattgatctt ttagtttctt cactaattct tcattcaaat caatggttgc
      901 ttggaaggat tcaatctgtt tttgtgctgc tttttgttct tttttgtatc ctttcacttt
      961 gtaattgatc gcatgttgtt cataatcttc atctttcact tgatctttaa ccttatcttc
     1021 gtgaacgtaa taaatcagat cttgcggttc ttgcatatcc acttttggat caattttttc
     1081 aggaataatg gttaaacgca tggcaatata ttcttctggt acatgagata gcttcactgc
     1141 aaaataatca tcagacactt tgatcacttt ggcttttatt gcttgataat cccctttagc
     1201 tgtctttact tctacgcgat aattaagatt agccagttct tttaataagc tttgatcttc
     1261 atcgtcgatt gcaaattcaa aaactaattg ctggttcgct cctttttctt ttgaaaccat
     1321 cgtcagttcc ttcgtgttta caaatgcgtt cgtgcttatc tcatctggta tgtctacctt
     1381 cttatgcgta taaattggac tagcaagaat caaaaagccg agaatcataa agatccccag
     1441 ccatctaaga taactttttc gttttttgaa acgttgtctt gctccttttt tcttactcat
     1501 tttcttcctc ctaacgagtg aaataactaa agtctgtgac tttccattga ccatctactt
     1561 gtttttcagt gacagttaat ccacctttta ttttttgcgg tttgacatct ttgaattgag
     1621 gattgccgat tgtataatca acaaatacga caagattttg ttcctctttt acactgcttg
     1681 tttgagcgta agtttgcacg tcacctaatg taaaggagta acttccgtaa tcatcatgtt
     1741 ggtgatcatc gtctgcagga cttaatttgt cgatcacttc ttgggttgcg acatctttta
     1801 gtttttccgt taattcattt ctgcttaatg atttatcaat ggccaattga ccgaactctt
     1861 ctgccgtctt tctttgtgct tcagttactt cctcttttgc ttttgtgcca tcttgttgct
     1921 tcagctgatc gatttcttgg tttttctcat tgatggtttg atttaacttg tcgatcttcg
     1981 cctgtgcctc agaggaattt tttttgagct tctcagacgc gtttttagcc acttcttcaa
     2041 ttttaaaggt atttgtctct tgttcttctt tcagctgcca aaacaacgcg atattgccga
     2101 ttaaactaac gctaattgca gctgttaaga cgatttttaa agttttattt tccatatttt
     2161 ttctcctgat ctattttttg ctgctattca aaaatggtaa tggatcaatc catgtaccat
     2221 cgtctgtgaa tgcgtgtcct tgtgcttgac gccaatccat tcgagtgaaa cctaaatgca
     2281 agtgtgctgt atctcgtatc ccgattactt gaccaacttt cacttgatcg ccaaccttca
     2341 cacgagaatt tcctgtacta ttcgcaaatt cttgatatac catattcaaa ccgtcgtaat
     2401 taatcacgat cacacatgca cctaaacctg aaataccagg attgccaacg tacacgactt
     2461 ttcctccatg caccgcgtga atttcacttc ctggatgatc gactgaacca aagtctaatc
     2521 catcatggaa gccattcgga cggaattcac cgcctggatt tgttccaaat aattgaccac
     2581 cactgaagga atttttatct aaactgctgc cagggaatgg attcgtccat tcaccagtga
     2641 tcgtgccgtt gttattgcca ccttcacccc aatgtttgat ggcgaattca tcatattcgg
     2701 ttaatttatt agaagtgata atggacatac atcctgtata atacggacta tcttccgtaa
     2761 ttcctgcagc ggcataaccg cctcgtccta agatcaataa acttgcgcca gggcttttat
     2821 ttcctacaca tgcattaaaa ttggattgtg tggctaccat aaagccgtaa tatgagaagg
     2881 cagcttttga gttgggaaac ttcatgtacc agccaccttc aatgccacca attcctcgtg
     2941 cagttccttt aggaaacaga caaccatcaa accaagtaat cccaaaatag ttgagatcat
     3001 ttcttgcgga cgccgacgta ccaaacgctg attcataacc gtattgcgaa aggatcacac
     3061 ttggtaataa tttatatttc actgctgcac ctacagcata ttgcgcaatc tcatcagaaa
     3121 aagggcctct tcctttgatt tctttataaa tcggtaagcc ttccgaatat tctcctgtaa
     3181 aagcttcgcc accactactt gaatctgaat cagaattatc aatcgaaccc gccgcaagta
     3241 ttagaaaaag ggtcacgaac acagtaatca aaatagtaat caaacctgca gaagtaccaa
     3301 acaaccacaa aaataagcgt cgctttgcac gacgcttaac atactgcttg attaaacttt
     3361 cattttggtt ttccattatt ttcctccatc aaacaatttt aattgacgtt tgttcggact
     3421 aaaatgaaca cgaatggttt cttttgagcc aaaattgatc acagcgtctc cttgattttg
     3481 ttgaggaatg gattctaatt ctgagtttgt cattgattta ccaactaatc cttctatatg
     3541 acgtaaaata gattcatcca tacgcataaa gatcttcgta gaagaaaatt caaagacttt
     3601 cttcagtttc gagaagttat cagaagacat attttctggc accatttctt gtggggattg
     3661 tgtagctaaa acagtcattg cttctacttt acgcatttct cgttgaaagt ttgtgacata
     3721 atttaccgtt tcaataaaat ctggattaat cgcattatgg cactcatcaa tcatcgccaa
     3781 gaagcatttg aagtcttgtt cttccatttg tttttcttta attaaacgtc gttgtcttct
     3841 tccttgaatc aatgcctgat tccaaatcaa cgtcatagcc atgtacattt gacattgttg
     3901 taccccacga tctaaacctg taatagagtc aatgtcaaaa aagaccaact gttgttggtc
     3961 aaggtgtcga atcgtactat gaccatcaaa aatcatgcca tacgtctcac ataaggattc
     4021 tacgatggat tccatttctt cttttcgatt ctttttctct tgggaagcac ctggtaattc
     4081 cagttgtcct aaaaagtcta aaaattcttt gaacgtcgga taagcttctg gatctttccc
     4141 tgtgacttgc gtctccgtat ttgaacgcga gtttgaggaa gtgaaaccgc gtggtactgt
     4201 tccatactct tcataaaaac gatttaaata ggtcttaaat tctgctttca tcgttccttt
     4261 tagctgagga ctcactaact gtacctgata gaccactttg tctaagtgcg tttgatagga
     4321 cgctgcttca ttgatcttca aatctttgac tgatccatcg tcatataaac tggaagcttc
     4381 atcatacgta cgtgtggcaa atatttctaa aggattgatc atcccgtcag atccgtctaa
     4441 acgcaccata atgccgttat attcctttaa aaagggaata aagtcttttc ccttttcaaa
     4501 gccccaaata aagtctcctg cagctaaatg ggcttccgcg atcattttaa gaacggtcga
     4561 tttaccagct cccatcattc ctagaagcaa ggtattggta gacaaccgca cactattttt
     4621 atacgtggca ttccaaacaa acgggccgcc tgtcatggtt tccccatatt gtgttccacc
     4681 tggatctttt agcgtttggt ggtggaacgg gaaacctccg ccaatgttta gcgatggtag
     4741 tgattgccct ttgatatttc caaaccattc agcttgttca tcgaacatgg tgaacatcga
     4801 ttgccagtct cttttcgttt tgtgcagata ggtcactgca cgataatcca tccctttgag
     4861 attttcgatc agttcctgtg ctcgttcatc tacttcttga atcgtgtttc cgtaaacatg
     4921 tactctcact ttgacttgtt tagaaatttc cccattagaa gtcaattttc gggcatattc
     4981 cgctagttct aggtattctt ccgcgctttt atttcggtca gtcgcttttc gttcattatc
     5041 tgaccgatca tttaattcat ctaaggcacg attgatttga tcaatcactt tttgcttttc
     5101 atctgtgcta acatctagtg ttgccactgt atgaggattg ttcatgagat tggccaacca
     5161 aaaaagcgta ggattttttg tgtagtgata aactgtcaca catcgtgaat aaccatttcc
     5221 ataggtcaca tactcttcat cgaaatttaa tccaccttgt ggcatcactt gttttaaaaa
     5281 gtctaaatca taccctttgg attttaaaat ttctatttcc cgatttccta gttgctttaa
     5341 tggatcaagt accttttgat cattttgtgg tgaaaaagct gttttaattg cttctaacat
     5401 gtacagtcac ctcttcttgt ttgattcttt acttggttaa aagattttcc ttttcccaac
     5461 ttacccaggc ttaaagtgtg aggaaatttt ctagtggtct cttctttcgg aacatctttt
     5521 tctaaccata tcaacggata tttcattctc tcttccccat tcctactcat cattcatatt
     5581 attcattcga taaagaatct tgatcaattc ttttttgtag agtggtttta accctaaaat
     5641 gccactagat cgcattaact gacggtttct ggtttgcaaa tccttgattg tctttccttc
     5701 aatgacgaaa aagaagctta aatcagggcg ttcctgttct gctcgtttca attcctggat
     5761 ttcctctact aatttctttt catattttct taatcgctgt agatttcttc ctaaggactg
     5821 ttccatacga tttttatcta ataactgccg cttttgcacg atttgctccg ttagatccgt
     5881 ttctgtggtc aaactaacta acttaatcgt tggcacatac gtacgcatga cagtggtcaa
     5941 ctgattcatt cgttccagtt tctcttgttc atttaatcct tccacatctg tagaaactac
     6001 acgataataa cgttgaaaat gatcttcttt agtaattaag actccctctt ctgtacagga
     6061 tttataagga aataagtcca agatagagcg ctcttctggt tttccaaact gcaaaatgga
     6121 ttttctgtca actgaatttt gacctttgac tcgcatttat tcatcctcct tatacgtacc
     6181 atgataattt cggttaggat ctaacgcatg ataatggttc ttgtccgtaa aaaaaataga
     6241 ctcgataatc gcaaaaatct ggcgttgacc tgggttatta tagggtcgcc aaacaatcac
     6301 taaagctatc gcaatccata agccataatt caagagtgca aaaaagagtg gtaagtggac
     6361 aagattattg actattttcg cccaaaaaaa gcaaccacca atcgttaata gatctaaaaa
     6421 gaaaaaggcg aaaaacttcc attccccact aaatcttggt ggatttttca tatgaatgta
     6481 tttatccata atgtaaccat actccttatg ttctttattt ttagaaacaa aaaacactgt
     6541 gatttctcac agcgtttttc taaatattat aactctaatt caattttctt cttttaattc
     6601 aaaaattttt tgttctaggt ttcttttcat ttgaatcaac tcataatttt gtgcgcgtaa
     6661 ttcttcgttt tccaaatgaa ctcgttgcat ttcatcgttt aatcgattta aattctccaa
     6721 tgattcttgt atcgtttctt gcctaaaatt aagtgagcca agttgacgat ctttttgttg
     6781 tctttgctct ttcaattctg acgatatttg ctcaacagaa tgaaatagct ccgttaattt
     6841 tactgtgaat gtgggcatag catctttcaa ataagtgaat ccttctgaaa ggtgtacaat
     6901 aatgctttcg gtatgtgttt tttgaccatc taaaatagac tgatgatctt tttgtaaact
     6961 ctccgtatct ttaaatcctt taatagtcgc cacaaatatc gttaaaaatg ctcccagtgc
     7021 agttataaac gtagctatta aaatatcctc cacaattttc tcctttccat tttaaacttg
     7081 ttttataaag aaaaaggccc ttaaataggc cttttatggt ttgatatcct tcaatagttg
     7141 ttctttcaat gcattgttcc tgtttttggt atctcttgtc acatttattt ttgtcggtgc
     7201 agtaaatgcc acatttgctg gtttgtaacc tgtgtgaact ttttgaatag gttctggtgc
     7261 agcttctctt gtcacaacat tttgaacgga cgcattccct gtctccccac tttcagataa
     7321 attgtaagaa acagttgtca gatttccatt ttcaggcaaa cgacttggtt tcatttgaac
     7381 attacctgca gttgattggt gaacaaagct ttgatcattc tccttgaatt gattcaaacg
     7441 actatctttc attggtaaat tttgtgcctt aggtaaacca tttttcgttg atttttcagt
     7501 tccaacttcg accccattat catttggcag atcgatggaa tcatcatccg tttgtacttc
     7561 ttgatttcgt tttggtaaat ctgaaatgcg cttttcattt tcagaattaa tgttagattt
     7621 ataacgatat tgttctggat ctgtttttgg taattgacca ttttgtcctt cttctgaatt
     7681 ttcgttagaa tgcaactcat cttggtgatc tttggctgaa ggaatcgctg caccttcttg
     7741 attttctttt tcggttgttg tattagcttt tactgctgca ttgacttgtc cttcaccata
     7801 cgcatctttt agtccttctt tgacttctga agctttttct ttttgttttt ctagtgtttc
     7861 ggatggattc ttcacagctt cagccaattc tttagctctc gtgccgtcta atttctcttc
     7921 taaggtgtct ttggcttttt ctgtaagatc ttcagctgtt ccttttaacc cacgttcatt
     7981 gacgtaacca agaccttctg cggctttaga aacaccattt ttcgctttcc cgaacatttc
     8041 ggaaactgtt ttcttactat taggtacagt tgtagcttta tcgtctgatt gttcttcccc
     8101 gttatcaggg gtaacatctt ttcgattacc aaatccattt ttacgaatat catttaaatt
     8161 gcttaaacca ctcttaccag cacttgcaag gtttttagcc ccgctaccca catttttcgc
     8221 aacatttcct aaggcaaaca tgctaagaag agaattgctg ccgtttttca gactcgtatc
     8281 aacgccgaaa attttcgtaa ctgattgaga gcctttgaat agcgcaatgg ttaatacaat
     8341 taaagcgaca acatacaaga atccaatgat ctttcctgcc gcatgcaaat caccgatacc
     8401 tgaaagcatt aatgtataga ttcgtaattc taaaatcgta aatgccaata acaagaagga
     8461 ttgagaaata tcttctatag cacttttcat ccgttgtcct gtctcaaagt ccgttgacgc
     8521 ggcaatcact aaatagaatt ttttaaatac cagctcaata atgcaagtaa caatcgtaaa
     8581 caagataaat aaataggcta ccgacaaaga agattcacca gctaaaataa tacctggact
     8641 actaatgtat cggcgatagc ctgaagtata cacatatttt gctaaacctt catcactaat
     8701 ttgaacaggg atttctttcc cattatcgtc taactctagt cgatactgta atgcctttaa
     8761 ccgatctttt tgtactgtac taatatcact tgattcagac attttcttag cttgatcagg
     8821 tgtaataatc ccattgatat tcgctgtttt aaaatttttc gccgtgagag catttctttt
     8881 gtctgttggt atgtgttcta ctttgttcgg atcattttct agtaacgtac tcacatcaat
     8941 cacattattt tgaataattt gaaaagcagg tgaaccagtt ttatctgctt tatttgctgc
     9001 agtcacgtct ccgtaaaagt ttttactaat atttagccct tcatcgatta aagaactacc
     9061 accaaaaatc aaaatcatgg ccataaatag attaaccaag atatttttga tttttggtgg
     9121 atgtttactc ataaaaaatt ttaagcctaa ataaattaat gtgacgacac aaaaaagagc
     9181 caatacctta ctaatcaaac tttgaaacag ttcattaaca ccagcggcgt tcaataagtc
     9241 ttttaaatca aagctttgat aaattgtttt ttctagtaaa gtattgagat tatataaccc
     9301 tttaatcaaa ccccataaaa aagtattaca aatccaactg aatacatctt gatactgcag
     9361 ataattacca taatcttgta agactttcgt tacggttttg ggatctgcat ccccatcccc
     9421 aaataagcct gaccaccagt cggcaatgcc ggaaaatatt cctccaatga tcattctttc
     9481 accgcctttt cagcttctag caaaagtcgt ttccttataa cctctgtaat ttcttcattt
     9541 tgacgcaatt tttcaaggta ttcttgcaaa cgatctattt gcttctcttc ataaaattga
     9601 ataagtaact tttgtgccgt tgagttgtct aaagattgaa cgattttaat aaaaatgttt
     9661 tctttagctt cctcttctat ttgatcgtgc atattgtgat aaagtcgacg tgcatagata
     9721 tcaggtagaa aataatcgta atcaatttgt aaagccttaa gatccaagtc cgcatgtgta
     9781 caatgtatgt ctatatcatt gatatcccac gatggattga aatcttcaat atattgataa
     9841 gcataaggaa gattcgtttc cccatgatta aaaataggat acggccgtat tttatgacct
     9901 ttcaaatctt gtcgttttaa aggagcgagt acaatcgttt caccttctaa aaaagtagaa
     9961 attctttcag gagttaagat gcgatctgaa tcaacattgc gattgatatg agtattcata
    10021 cctaatgaat tgatggtact gttctcttgt tcaaccgtct tattccccac ttttttggaa
    10081 aattcttcta tggtattatc gtctgtcgac ataatataaa tatgtgtctg acaattttct
    10141 ttgataatcg ctgcgttttg ttccccatac ttcgcataaa gttgctgata ggattggata
    10201 aacaaatccc acaacatgtt tctaccagct gtgactgtca tgattccttc catattatcc
    10261 attggtggca tattcccaaa ttcatctaaa ataaaatgaa tcctacggtg acatttatct
    10321 cctttagtct taacacattg cgtagataat tctttataca actgactaag aaaaatacta
    10381 acaatgatat ggttagatgg atcataatca ggtgtgagca taaaaatagc cattggttta
    10441 tcggtatagt gcatccgaat agactctact aataagttac ttttatcctc atctaatgtt
    10501 agaacggctt ctttttctaa ttctggcaaa tgggtttctc ctcgtttttt tcgataaacg
    10561 atatttccct cttcgtcttt caatactctt tgagaaaact cataccaagc aacatattct
    10621 tttcctcgat gatcgtattt taagcaaaca tgatctccct gttctaaatg acaatcaaag
    10681 ttaatttcac aaaaacctct atagcctact ttgacacgtt cctcatgaaa tttttttcct
    10741 tgatgataaa actctacaac aattcgctgg tttaacagac tctcgtgtag tttgagatcc
    10801 aaatacttcg gaaaaccaat ggacttataa tcaatggttg attgagaagt cattttcgca
    10861 attttcgtca gtgtaaatgg atttagtcct gtgatcgctg tcgatagaat gttgcctttc
    10921 gctttatcag aaccaatttt tgttgcccca tattgttttt ttgctgtgtt tccaggtggt
    10981 aactgattca taaattcatc taaagcattg agctgcttga attcattatc aggatcttgg
    11041 taattaaaac caccaatctc agaaagaagt tcgacgagat tattcggtgt taatttggag
    11101 taatcccctg tctgatccgc ttgttccaat aacgcaaaaa taagcgcatt gacagctttt
    11161 tgcgcatttt cgttaaagta tttattgtct gaagcgttgg catcataata aatcgtattc
    11221 gtgaaagtat tggttaactg tgtcgctgtg tctatgtctc ctcgcttcca gtatttcacg
    11281 atgagtgcca atggattatc actcattgat tgcagtggat cggtgatatt aaatgcatac
    11341 acatcatacc ctctagcttc tagttcatct ttactagcaa catataattc tgtttttgga
    11401 tcattaacaa ccattgaact ttgtctttct gcacgagaca aattgtcaat ggttgctaaa
    11461 acttctatct gtcctttccc tgaacgggaa gtaccaacaa tacaagtatt tactgtatcc
    11521 gtctgaatat accagtactt tttataatga gtaattggta ttccgccata accaggaaaa
    11581 cctccctttg attctggtat ttgcggataa atttgttgga tttcctttaa cgtttttaat
    11641 cggttatctc ctttttgccc atctgctaga ggacgaaacc acatgtccat tttcaatccc
    11701 ataagtacag ctggaacaac acaaatcgta gaaacaacta aacgtgtgcc taaacttgtt
    11761 gttggggtca aaggattcca aaaaccagtt gaatcaaaat ggaataactg cggcatttct
    11821 tgtaactgaa aaatattcac tccttgtaaa cttcctggaa taatctcaaa tcgatgaaat
    11881 gcttctggta ttcttgtaac aacatagttt aaaggaagat aagctaagcc actataaaaa
    11941 atcttttgag aaatacgttt gtcatgtttt tctggtagct cttctcctga aggagtatag
    12001 cgatagaaat gagaaagata cctattcttt tctaaattga atcgtttgtc ttttcgtctc
    12061 gttgtttctc taacacgcat tatttcacct catttgattc tgaactttcg gaagtgtttg
    12121 attgtacagt tgaatcgctt gatccttctt tagccttact aggatcactc tctttgctat
    12181 tttccatact tgattctgtt gttgactgat tggtacgatt caataattct tctaattgtt
    12241 gttcatactc ttttcttgct tgttcttggg cttcttcctt gattgacgtc gttgtggcca
    12301 cagaatcagc ctgtgaagat tgattgatat gattaaaagc aaatactgtt cctccaccta
    12361 gaagcaagct taaacttgca atacatccca ccatccatcc gttaaaggaa cgctgtggtg
    12421 aaggagttag atttgtttgg ttgttcattg gggtttgaac agcagctact gaaggcgttg
    12481 gttgaggttg tgctagataa tttaactgag cttgtcgcaa tgtatccatt tgttcttgac
    12541 gttccttacg agcagctgct tctttttcct tttcttggtt taaccgttgt ttttctattg
    12601 ccatttgttg ttccgcaaga tcctgttcac gtttttttag ttctaattgt tcgcgctctt
    12661 tttctttttg ccgttgtttt tcttcttgtt ctttcaattc ttgttgttga atttcatact
    12721 cttttttcca ttttggaatt tgcacttgta attcttgctg gatcttttcg tgtttttcca
    12781 taagaacttc agtcgttgtt tgatatagct tattcagttg ttcatttaat tctttgctaa
    12841 tctttagctt atctgtacgc aaacctcggt aaaattcttc ttttcgttct ttaatacgtt
    12901 cttttaacaa gttttctttt tctttcgata acgcttcttg attcgtctga gtaaaattat
    12961 tgatctttgc tacacacaag gaatacgcat tgtcgcgttt cttttcgtat cgctctttaa
    13021 ttttttgtac ttcttcttca atcactcgct gttcttcttt atttagttcc tcttttgctt
    13081 tctcccaatt agacgactgt tccgtcgcat actcattaat ttcttgatct gtttcttgtt
    13141 gacgttcttc aaaggtggct aataaatctt cttgtgcaag ctcatgccaa tttgttgatt
    13201 ggacagactc taactgttct aaaagtgtgc ctttaccctg tgagattaat tcttctttac
    13261 tctctaaaaa tgcttgatag gtttggggta attgatattt cgaataaagc tcatttaaaa
    13321 ataatttgtt tttttctttc atccattcat gctctggcgg atattgatcc aaaatataat
    13381 caaatacagt tgccttttgt atctctattt cgtttttttc tacttcttca ctgataagtg
    13441 gttcaatata ttcctgagaa tccccttcag tccatgttga ctcttcatta gcttcaactg
    13501 gatcaacttc tacttcggcc gtctcttcat cctccattgt ttcttcaaag ggatctacct
    13561 ccaattgacc catcacatcg ttctcttcct caaatatcga tttctgagtc ggaatatcag
    13621 cttgttcgtc aacatagact gtctcttttt cttttgactc cttctttgga aatattggtc
    13681 gttcccatgg tacttcttca tttacttcga cttcactccc ttgattcact tttctttggt
    13741 cgctctctaa ctcttcttct aaaacttgta aaagtttttc gtttcgataa gactcttcca
    13801 cactcatagt ttcatccgtt tctttaaaca catcaaggcg gattaaatcc atcaatgtag
    13861 gatagaaacc ttctgaaatt tgataatcca gttgcccaaa aacgtattct gtaccagctt
    13921 tctctatttt tccttctgtg tcatgtaacg tgagagtcaa cttaacagaa gatcgacttt
    13981 cgtgttccag tactttccct aacacatgta gctgttcttc cgtttcaatg acagaatatt
    14041 taattgtttt cggtaaaaaa gaagtacgct ttcctacttc cttcgttgct ttttttatta
    14101 actctttatc tgttttggta ttgcctgcaa tcgtaaagta catgagtatt ctcctttcat
    14161 aaaaaaggag taaaaagtat cgactacttc ttactcctac ttttttactt tcttataatc
    14221 cttgaggtgt ttgtccaaac aatccaaata acgaaaccac aatagaagct gctgctaaaa
    14281 taactcctac ccccatcaat acatagccta cgtgcatctt cccattttca gctcctcttc
    14341 gtccaaatag gaagaaaaga cccccaataa caatggaagc aacggccgca gcagcagcaa
    14401 aaatcatcag attgtttttt aaactagttg cggcagtttg taatgctcta aaaatatcaa
    14461 ccgcagctac tactttcggt tgtgttaaaa aattcattgt aattgattca aacataaaat
    14521 tgttcgctcc ttttagtgtt taataatttc gtcgaaaaca agcttttcac gttcacgttg
    14581 atagatatat ccttctaagt ccttgtcttt tccccgtaat aggtcaaatt gtgctttttc
    14641 aaggacttct tgtttccact gtgtttcaga tacaccgcgg cgttctaaga ttttagacgc
    14701 ggctttttca aatggcgttt tagcttttgc cactgtgatt cctccttcag gacaaataaa
    14761 aagactagca atcactcgct agtcttttgt aacttatatt tattggattg tgatccatcg
    14821 tgaatcaatt tctctttcgt tcgcttttca tccttgctag atccaagaaa aattattttt
    14881 tttacgccat tcttctcata gggaacaaca ataaataaag aatcagattt gtttataaat
    14941 tccctaacac cagctgaatc tactattttg tatctttcta aacctttata gtctttttgc
    15001 ctcttgactg ttatttgtac aggatctgga tgccatacac tctcagagta ttcccaggta
    15061 gtctcgtcta taattttcaa attataaaca agaccaccta cttcttcttt ccacatagtt
    15121 cctgatttca atacgccatt atcttgatta gtttgtttat ttttttcatt actacatccg
    15181 actaaaataa caatcatggc aatcaaggaa gtaactacta ttattaactt tttcatttta
    15241 ttcattcttt cgttttttta ttttaaatgt atctcagaac cgcataacta aaagttgcta
    15301 acacaagtaa tagtaagctg accgctgaaa tcgttactaa atgtgtagaa cctgttttag
    15361 gaacaatgac ctgtggcaag ttaggatcaa ccccaattgt cacaacattt gttttattag
    15421 aaacattatc tactgctact acggcttggt tatcaatatt tttttctgat tgtaatggag
    15481 ttttagctgt aaatatcagc tgaatttctc gtccatctaa aagattgtta cttaagaagt
    15541 cagcggaagc ttcccacttc agcgttcttg aattgctatc ataggttagt gtgccttcag
    15601 aagtaatatc ttttccttta ctcttatcta ttacttgcgc agaaccttct ttatatgtca
    15661 aacgagcttc aattggatct tgaatactaa attgtttgta gtgatctgac gcagcgtcta
    15721 cgccttttgt gtgccatttt tgtgtgacta gatattgtac ttcagatcct gcgtttaaag
    15781 gagctgtcgg cgtacccttc ttaccgtcat ttcgtccgtt tacatctttt ccattttgtt
    15841 gtacttcttt cactggaatt ttagggaaat aaacggtaat gatatttgta tccttttcta
    15901 ctttattaat gatcgacgtt ccctgatttt tggctacact attttgattg gccaattcat
    15961 tgacttttac attgatcact aattcgtaag tttcgccgtt atacttcatt cgatttttta
    16021 atgtatcagc tttcgctgca tattttacct ggtgttttgt tttgtcatag gtcacttcac
    16081 cagatacatc gaatttttta tttgtaccac tttcaacgtg tgcatcgacg taatttactt
    16141 cttttggtaa attatcgatt aactcaaatc gatcgtaaac cgctagtaaa tccactccta
    16201 atcgattcac cttctgtttg attcgatatt gaatcgtgtc tcctacttgc aacattttat
    16261 cgttcacgtt atttccattt ttatcaaccc ctgttttttc aggttgatca ggaacaacac
    16321 taaagattgt cgcagaagat aaagtaaacc aggtttgaac attagaagaa ccaatcacaa
    16381 acgtcggatt tgcttcagat attttaaagg acacagtacc ttgaccaaac ttcggatctc
    16441 ctaatttatc atgcaaatta ttgaaatcat tatccgcttg tgtcagctta gagcttcctc
    16501 ctgggtggcc acctaccacg ttcagattgt tgtagaaaga tttaaattct gaaagaaccg
    16561 tatcttttct gacataccaa tctgtcgtat tcattttgtc atagtgggca tattctcctt
    16621 taagatccgt ggtttttcct cctgcagggt tcaaagagtt aaaactgata aatgtatctc
    16681 ctgaaaattg aacggggcta ccgtcctcat atgtggctac tagctgcaca ttcatttgtg
    16741 ttgaatggta aacaattcct gagaaaagat tgtctgagat ccaaagtact gttctttttt
    16801 tagatttact cattgcacca ttcgtatttt tatagccatc gtctcgaaaa tgagtttttg
    16861 tatagttgtt atctagtaga ttccacggca acgtatccga gtataagtga atattagata
    16921 gaactagctt gatattgact tttttcccgt tatacgtacc gatttttcca tagtcaatat
    16981 tgactttggt gttggttgtt acgccttcta aaacaaggtt tgtttcaccg tattcttttt
    17041 ggaaagaagg atagcgtata tttttgtttc cctccactaa tcctacgtag tcgtctgtaa
    17101 aaccaccttt gttggcatca acatgatatt tgtaaacttt tgacgctccg tttacagtaa
    17161 tatttgtttt tgcattagga acatcttttg gaataataaa attattcttt acgttctgga
    17221 ttggatttga cggatcgaca ataacttttt cagcaaagac attggctgtt tttaacccaa
    17281 acaatgtacc taacatcatc attatgggaa aaacatattt taaccgtctt gtccacgagt
    17341 gtttaaaatt catcccttca tcccctcttt tttataaatg actagtgaaa ttccgacaat
    17401 aacgatcaaa ttgatcgtag taaataatgg attcctaatg agcttttcca taatatctag
    17461 cggcatggaa gcagctggta tgtttaaaat cctcattagg tatagttggt agcttggcaa
    17521 aatagatagt ttagaaaata ataaaccgca gtacaaaaag actgcggtta aacaagaaaa
    17581 attgaataat aataatttga tcagtttcat tttatttctt ccttaatctc tagaaaacaa
    17641 gtgagtaaca aaatcccaac aattctcaaa gaagtttttc tttacgccag tagaaacgat
    17701 tttttttact tcttctttag gttgcgaagc ttctgattta ggatagatcc acacatcctt
    17761 taagaattca ccgtttttat cagaaacagg tgttaatagc aaaatatcct cgctattaga
    17821 aatgttttca ggacttgatt cattgacaat ataataagct tgtttctgat ttgcctgtac
    17881 ttctatcgag aattcaaaga cacctgtttg ttgatcaatt gtctttgttt ttccattaga
    17941 aactagcttc aattgatcac tgaatagttt tttcactcga tcttttaatt gggattcaat
    18001 ttggtcagta gtgagatcct taacatccat cttttgtaaa atgtccgata catcataaac
    18061 agaaaacttc gcgccattta ctccttcttg tttttcaagt ttttgcccct ggttgtctaa
    18121 ttgttcggaa gaaataattt tttgaacgtg aatggtctgt ttgactattt gatcgtcttc
    18181 agcaattttc ggttcttctg aagaatcaac tgttgacgaa tcgacggttt catcgctgct
    18241 ttgaatcgtg ctgctatctg acgtttggat tgtttgttct gtgctatccg tgctttcttg
    18301 tctgtctgca aacgtcaact gtgtgtaggc agtgaaagaa aaaagagcgg ctgcacaaac
    18361 aaatagccat tttctatttc tcatgttggt taccctcctt tattttttct ttttataaac
    18421 aaatagcgaa ccaatgatca tgactgaaag aaaaacagta cttcctaaaa gccatgtcca
    18481 agataatttt tgtttcttat tttcttcatc ttttaattct gattttgtaa aggttactcg
    18541 cttcccatac actagaaaac ggtgtgtgtt gatcccctta ggcgtacatg tcattagcgt
    18601 tactcgatct tcattgggat ttggttctaa gtaagtgaca aagtttcttt gtagttcgtc
    18661 tttttgcact tcctcaatcc gatcgatttg ataagcgtgc tgctcaccat caactttgat
    18721 ataaaacttt tctccctttt ttactgaagg taaatcatca aaaagattat ctttataaag
    18781 tccactatga cctgcgatta aaggattttt accgtttcct cctgtaatat caccagttcc
    18841 ttccgtaata cctacaccat tttctaaaat tttgtcggac gttccgtcat aaattggtag
    18901 ttttaaagaa atacttggaa ttgtaagaat accaacagga tcgcctaact caatcttagc
    18961 aacatccaaa gaagaatccg tttctgctgc cgttcctgtt cgatcgattg cgacaatctg
    19021 ctgattttgt tggtatttcg ccacgctcgc ttcagcaggc atcaaaagta agacacctac
    19081 agctgaaagt aaccagaaag caatgatcca tttacccatg attcgctttt tcttaggctt
    19141 cctgataatc attggttttg atccttctag gctttcttct taaagtaaac aactgcagaa
    19201 agtgcccctg ctcctagcaa caagcaagag attaatacat acgtttgaaa accgttgcta
    19261 ccggtcattg gtacggaagc tttttgattg tttttgatcg tgattcggtt ttcttctttg
    19321 tatgaatctt tgtctacttt gaaagagatc tctttcgtta atttttgata gttttcaggc
    19381 gcttttgttt cttccagtga atattcacct tcagttaaac cagtgatttc aaagtgacct
    19441 tctttatctg attttaggat cgctgcattt gctacatcgt ctacccatac tgcttctttg
    19501 acaccgtttt tatccgtatc aactaatcca ccataatatt ttccttctgc gtttttagca
    19561 gcaaattctg cgccttcaag cggtgttgct ttgattccta ttccaaatag tccgctatcg
    19621 acttttaaga attttgcacc ataagtcaca acgtcagctt tcgttttttc gtggtgttct
    19681 tcctgattaa atggattgtt gttataaacg aaatccacgc tatttgttaa tggattggca
    19741 acagctgcgc tatcatttac atacatgtta taagtcattt gaagatcttg tccgtgtaaa
    19801 cttgctacag tatcacttac ttggccatta ttcacgataa aatcgatcac aaagccattt
    19861 tctgaatttt ggattttata atctgtattt tcttttaata ccacagatcc atctttattt
    19921 gttactttga tatttgcttt agcagcgaca taagaaagtc cttgtcctgc tatatccccc
    19981 catttaaata cactgtaagg agaagtttcg cttttcactc cgtttactgt gataccgcct
    20041 gctccgcctg caatgtctgt tgggatatga atcgttcctt gataatttac tgcgtcacca
    20101 atttggaaag aatgttcttg tcctgttcca tttgttactg ttttatcgat cactggtgct
    20161 ttatagttta cgtatgaagc ttttaagtca cttgtcacgc catcattcgt aatttcaaag
    20221 gttaatttat tgtgcgcatc gttgcgagcg tctgcgccta ataaatattg atctgctgtt
    20281 ggttctttat ctgatcccac tacaacttct gaaggaactt ccacaaaata gtattttcct
    20341 aacgtcaaat cagtagcagt cagctttcct tgatcatccg atacaagatc tcctaatttt
    20401 gttccttttc caggttcgcc cttgtataac tcaaattttg ccccttttaa aggtgtttgt
    20461 ttgctttgag ctgtgccatc gtcaccaaat ttcgttaatt caaaactcaa cttagaaaca
    20521 atatttttag gatataagtg aatgtctttt aaaaaacctg aacctgtatt atcagtcatt
    20581 ggcgcaatca caaccattgg ttttgctttt tgtgtgacta atccagcagc ggatttactt
    20641 tcataaacca catatacatt ccctttggaa ttgacatagg ctggttgatc agcaaaagta
    20701 atctcaccat tttcatctac agctgaagta attttattgg ttgcattctt gacgtactca
    20761 ctgttagatc ccttgtcttc tacatcttta acgatttcgt ccactttcgc gttggttaga
    20821 tcagaatctt cagtcggtaa tacttgatcc gtaatatttc ctaacgtaaa ctcaacatca
    20881 ccataatctg ccttgttgta tttcgttaca cctgtaggat attgatcgtg cgtataacca
    20941 tcgtttttga tcccgtcaat atcaacattc aattgcgttc cttgatcgta cattaattta
    21001 tgaaccgtga tattgacctt ctctggtggc gttttttgtg tgacatcttc tgcatatgct
    21061 tgatacccct gaaagcagct tgcaatgatt cctaacattc caacaagcat tccagctttt
    21121 tttacatacc gatttattgc cattttttta tctcctcatt tcatatttca acgactcgcg
    21181 ttgattaaaa ccttgttatt gtagctctcc ctgtaccacg tatctttctt ttccttgtga
    21241 cgtataaaca caagtaaaca acgttaataa gggtttctct cctttctttg gcaaagacaa
    21301 gaaagagttc tcttcaggaa cgccatcgat taatttaaag tactcaggta cgataaattt
    21361 acgcgttacc ttgtaacgat agacgccatc gttactaact aagtccatca cctcccctaa
    21421 tgacaacttt tgaatatcag aaaataaaac acctggcatt tccatgttat gacctgctag
    21481 gacatagttt cctttgccca tttcgccatc ttcataaaaa taagtagccg ctccaagact
    21541 caaagtgtat tgatttgtcc ctttaaaaat tggcaatgct aaaccaagtg caggaatctg
    21601 aatttgtgca aatacttgtt tattgacgcc catttcgatt gcttcttttt gtatggacaa
    21661 catttgaccc aaagacatat cattattgac gttcgttgca tcctccttat aactctttgc
    21721 gtgtgcattg atttgatcta cgggtatctt tttttctata ttttgcttaa acacctctcc
    21781 ttcatgtttt tggtagctaa catagccaaa cacactgcaa aagacgatta aagcgactcc
    21841 taaaaaaaaa ataaccattt tttcatccat ctgcctcctt tcagataaaa caaaaaacac
    21901 tttttcacaa cgaaaaagtg ttttctctta ttactattct atttcattta tctggtggtt
    21961 ttatattggt gtgtagccat tgctaacatt aagaatacaa ttggcaccca actgattgta
    22021 gttaatgttt cttccatgaa tgattgacac atgatcgcta aaaaagccca caagaatgtt
    22081 agttgtccgt ttgtcagctc acattttctt acgagcaacc ataaaaaact aagcaaagga
    22141 ataatcgata gagcaaaacc aatcaatcca tagtgattga cgagtgcgtg atacgtatta
    22201 tggttaccta gaatctgatt tcctatatgg aaaaagaaag gtttcatccc tacaaatatt
    22261 tgtcctgttt tttcgcctaa tgtttcatag aatttgcgcc aaatttcttc tcgtcctgta
    22321 aataaatgaa ctttgtctga cattgcagca aaataagaaa tcggtaaagc tagaaccgaa
    22381 actgctgcat acatccaaaa ggacaagtgt gtgtttcgaa aaatcttctt aggtaaaatt
    22441 tctaggataa cgaagataac aagcgcgtat aatgctgctt tcgattggca aacccaaatc
    22501 gttgccccag caagaatcaa cgctggtaat gatagcaatc gcatgtacca ttttacaaat
    22561 gaactagcaa atcctgtgat aagaagggct aatgtcatta aagaactacc tatcgtattt
    22621 gtattgatcc aaatatctga taatttgttc gatggattcc aaatactttg tccttctggt
    22681 actggtatct cggtaaatac tcgataaacc gttacgagta aaaagacata gaatactaca
    22741 agatacaagc tacgatcaaa cacagtaaat tccgtccatt ttacaatcaa aataatggca
    22801 ataattgtcg gaacgatcag atcaccatag aaagtatcta ctcggtagct tctaaaaaaa
    22861 gaaaagaaaa ggtaaaaaag aatgcctgct gcaatgaaga ataaatcgtt tctctttacc
    22921 ttgtacagat tcaaaagaaa caaacagcct acgaaaaacg tcactaactg tgcagcatta
    22981 tcataaataa tatcgtagga ggatttaacc ccaccgtcca tattaaatat agaatggata
    23041 ccaacagtaa cagaaaaatg gcactcattt tattgatcaa tttttctaca agttcttgaa
    23101 tgtgactcac ttcccttatt tattgatttt ttctacattg actaattcgc actggatcac
    23161 atcgcacttt ttcatattat ttgcttcaaa gtctaacagt gtgagtagtg gttttttacc
    23221 tgcttttggc aaggataaaa aggaattgtt ttccttagat tgaactacct ggctgtaatc
    23281 ataaattttt cctcttttgg agacttcata ctgatacttg taatgagcat cgattagatc
    23341 aatttcttct ccttcagata aatcaatcat atcggataat aaaacgcttg gtgtatctac
    23401 gttataacca gcaagaatat aatttccatt tcccatttca gcatctggat aataagtaga
    23461 agctactcct aaactaagcg tatcgctatt cgctccttga aaaacaggga gaataaatcc
    23521 tgtagaagaa aaaacaagtt ttccagcaat ttcacctttg agatcctttt ctaatgcctg
    23581 tttttgatta gtaataagtt gtctcttaga aatatccgtt aaactggtta aattatcttg
    23641 ataggtttta tcctgttcct gaatatggat tgtttttgtt acaccatcta ctttttcagc
    23701 gattttgtcc ttttgatgac cttgataagc aaaataacta aaaaggagaa aaacaatgat
    23761 tacgcccacg taaggattaa ataaagtttt attgttttta ttcatctatc taccttctat
    23821 atttgaatta atttacctac atctaaccaa gataaaccgc gttaaatttt actattcttt
    23881 ttattaaata acaatgccac atcataattg atcattttca tatcataatc gtcaagaaac
    23941 ttctaataat agttggcaat tagatcttat tttcacataa gttcgtctca actgttcaaa
    24001 atttttcatt gatcccaata gacaggcttt ctcctttcct gttatctgca tgtcactgat
    24061 atattgcatg acagataacc ttttaaggtg tttttgtttc atcgttttta gtaacttttc
    24121 ttccatttct ttcgacactt ttttctattt tcgatttctt tacaattcat gagttttcct
    24181 accttccttg ttttgataaa aataaaaaag cctatgatca catacgctct acacttcact
    24241 cttgggttat ctttttcttt cttttaacta ctggccttgc tggtttcaaa tccttgattc
    24301 cattatagat tctccaataa tcaaactctt ttcgtaatct ttctttttcc atatctgttc
    24361 ctttggataa ggctttaaat acatctttag gtgaataact acgagctaac agctgtacat
    24421 accttccttt tgtcaaagta tccatatcca tcgcatctaa aactggtcgc gccatctaat
    24481 ttgcctccta aataaattct ttactcgatg tctaatgata atactagctt tttattattc
    24541 gtcgtaattt ctgcacattc ttcttcatta ttttctgcga ttaacgcata atagcatggt
    24601 gattttactt cgaaaaattt catgatctat tcctccaatt tttattcttc tttatccgct
    24661 acaaagtcaa tgatttcaac actgacaata taatcactca aatatgacca ggtatcttct
    24721 acagacaatt cgtctccttt gtcgttcgta agcgtcattt taaaccactc atcgccttca
    24781 tcatcttcag gtagcaagcc gaatccaatt ggattatcag tcaatgttta atcgtataaa
    24841 tcatcctcgt ctaatgctaa aacatctaat cctttacaat ttccgcctct tctaaacgtc
    24901 acaaatcctt tgtaatccca acgttggaaa gaaaccttaa tagtatgtgt gcccaatcgt
    24961 atattcggat cataattttt catttgtcat tcctccatta cgtaaatttc attttctcta
    25021 tgttttatat caattttttc actttattca ggacttgttc atattttatc gtttttcgtt
    25081 tacttaaaaa tacataaaaa atttcgactc gtttatggcg tccgaaacga gaaaccctag
    25141 tagacagcgg taaatgctcg attgatccaa taatcaacgt cctcgcaagt atctacatgt
    25201 actgcagcac acgacacaat ttcttgtgcg tcctccccat tacgagaaga cctactcctt
    25261 attccatgag tttgcattaa agtctagatc ccttagtagc tgctcatgat taatctagac
    25321 gctattctaa cttgtctaac ttctaaattc cctcttgttt actctgcgct tattatttac
    25381 acaaattcat ttcaccacta tccaaacaag cgcatttact cgattgacca attttttacg
    25441 ttaccaatcg ttaaaaccta ccccgtcact ccccgaaggg aattgttgat agatcaagtc
    25501 tactccttat ttttctagtg actctaagtt cctttagaag tctcgacaat attttcaagc
    25561 aagcgaaaaa cgataaaata caagttatga gaatgatcaa ttcagctgat caaacgcccc
    25621 atcccttttg cggtacaacg caagactcca ttttttcttc gaccaatcag tacaccgttt
    25681 tggtcataaa catgtttgat tgttggatat agctctttta aggggatagg atcttttaaa
    25741 attatttgct tttgtttccc aatgtaataa atcatttcct ctgagacttt gtctttctgg
    25801 attatatatt tcttacactg cacaacggtt ttactttggc cagttgatag tttcttcgtg
    25861 attttctctg tagcggtaca ccgtttaccg tagtgacaaa tatctttgag actcaaaatt
    25921 cctgtatatg acataaaata aaccacccaa tttctttaaa tatttttaac aagtttttct
    25981 tgttattttt caaaaataga gaattgattt tcgttcatga caaacaaaaa agagggaacg
    26041 catccaaaca tgcgttccct ctaatttcag cgttc
//
LOCUS       DAZKNO010000019        25683 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000019 DAZKNO010000000
VERSION     DAZKNO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.019"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(174..1754)
                     /locus_tag="ACG8MY_000592"
     CDS             complement(174..1754)
                     /locus_tag="ACG8MY_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0429818.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2155..3531)
                     /locus_tag="ACG8MY_000593"
     CDS             complement(2155..3531)
                     /locus_tag="ACG8MY_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0429819.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3659..4747)
                     /locus_tag="ACG8MY_000594"
     CDS             complement(3659..4747)
                     /locus_tag="ACG8MY_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0429820.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4919..5353
                     /locus_tag="ACG8MY_000595"
     CDS             4919..5353
                     /locus_tag="ACG8MY_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0429821.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5431..5964)
                     /locus_tag="ACG8MY_000596"
     CDS             complement(5431..5964)
                     /locus_tag="ACG8MY_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0429822.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6058..7236)
                     /gene="mutY"
                     /locus_tag="ACG8MY_000597"
     CDS             complement(6058..7236)
                     /gene="mutY"
                     /locus_tag="ACG8MY_000597"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0429823.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7229..8026)
                     /gene="recX"
                     /locus_tag="ACG8MY_000598"
     CDS             complement(7229..8026)
                     /gene="recX"
                     /locus_tag="ACG8MY_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0429824.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8133..9515
                     /gene="rlmD"
                     /locus_tag="ACG8MY_000599"
     CDS             8133..9515
                     /gene="rlmD"
                     /locus_tag="ACG8MY_000599"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0429825.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9689..10267)
                     /locus_tag="ACG8MY_000600"
     CDS             complement(9689..10267)
                     /locus_tag="ACG8MY_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0429826.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10589..11059
                     /locus_tag="ACG8MY_000601"
     CDS             10589..11059
                     /locus_tag="ACG8MY_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0429827.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11167..12393)
                     /locus_tag="ACG8MY_000602"
     CDS             complement(11167..12393)
                     /locus_tag="ACG8MY_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0429828.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13147..13347)
                     /locus_tag="ACG8MY_000603"
     CDS             complement(13147..13347)
                     /locus_tag="ACG8MY_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0429829.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13481..14089)
                     /locus_tag="ACG8MY_000604"
     CDS             complement(13481..14089)
                     /locus_tag="ACG8MY_000604"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0429830.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14174..14644)
                     /locus_tag="ACG8MY_000605"
     CDS             complement(14174..14644)
                     /locus_tag="ACG8MY_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0429831.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14692..15606)
                     /locus_tag="ACG8MY_000606"
     CDS             complement(14692..15606)
                     /locus_tag="ACG8MY_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0429832.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15752..16555
                     /locus_tag="ACG8MY_000607"
     CDS             15752..16555
                     /locus_tag="ACG8MY_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0429833.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16733..17680
                     /locus_tag="ACG8MY_000608"
     CDS             16733..17680
                     /locus_tag="ACG8MY_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0429834.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(17748..18041)
                     /locus_tag="ACG8MY_000609"
     CDS             complement(17748..18041)
                     /locus_tag="ACG8MY_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0429835.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18069..18734)
                     /locus_tag="ACG8MY_000610"
     CDS             complement(18069..18734)
                     /locus_tag="ACG8MY_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0429836.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(18874..19506)
                     /locus_tag="ACG8MY_000611"
     CDS             complement(18874..19506)
                     /locus_tag="ACG8MY_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0429837.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19513..20580)
                     /gene="liaS"
                     /locus_tag="ACG8MY_000612"
     CDS             complement(19513..20580)
                     /gene="liaS"
                     /locus_tag="ACG8MY_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0429838.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20577..21308)
                     /gene="liaF"
                     /locus_tag="ACG8MY_000613"
     CDS             complement(20577..21308)
                     /gene="liaF"
                     /locus_tag="ACG8MY_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0429839.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(21476..21955)
                     /gene="greA"
                     /locus_tag="ACG8MY_000614"
     CDS             complement(21476..21955)
                     /gene="greA"
                     /locus_tag="ACG8MY_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0429840.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22091..23266)
                     /gene="mltG"
                     /locus_tag="ACG8MY_000615"
     CDS             complement(22091..23266)
                     /gene="mltG"
                     /locus_tag="ACG8MY_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0429841.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            23431..24591
                     /locus_tag="ACG8MY_000616"
     CDS             23431..24591
                     /locus_tag="ACG8MY_000616"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0429842.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(24631..25578)
                     /locus_tag="ACG8MY_000617"
     CDS             complement(24631..25578)
                     /locus_tag="ACG8MY_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0429843.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
BASE COUNT         7733 a         5231 c         4150 g         8569 t
ORIGIN      
        1 taactagcac cacgggtatt tttatatttt tttgattaga catttctatg attttaatat
       61 agtaaaaaga gccgtcacgg aaaatccgtc acggctctaa acaaaatatg agaggctgtt
      121 tttgtttggc tattcttatt acttgatgca gggcagcttg tgtcaagact tctttacatc
      181 aaacttttta attttacatt aggatattta tccgcaaacc agcgttctgc aaattggttc
      241 tcgaataaga acaatggttg gtcgaaacgg tcacgagcca agatattgcg acttgaactc
      301 atcttctcat ccaaatcttc tggatcgatc cagcgagcga ttttgtgtcc cattggagac
      361 atgattactt cggcgttata ctcatttaac atacgatatt ggaatacttc gaattgtagc
      421 tgaccaacag ctccaatgat atattcttcc gttagatatg ttttataaag ctgaatcgct
      481 ccttcttgaa ctaattgata gattcctttg tggaaggatt tttgtttcat gacatttttt
      541 gcagtcactt tcataaacag ctctggagta aatgaaggga gttcttcata agcaactttt
      601 agttttcctt cgaacaatgt atccccaatc tgataattgc ctgtatcata tacaccgatg
      661 atatcgcctg ctactgcttt ttcgacgttt tctctcgaat ctgccataaa ttgtgtgaca
      721 ttgcttaatt tcagtttttt atttgtacgt tccaaccaga catccattcc tcgttcgaat
      781 gttccagaac agattcggac aaaagcgatt cggtcacgat gggctggatt catatttgct
      841 tggatcttga atacgaaacc tgaaaattct tcctcataag gacttaccat ctgatcatct
      901 tgtgttttgt gtccataagg agctggtgcc aattcaacga aagtctctaa aaaagtctgt
      961 acaccgaagt tagtcaatgc agaaccaaaa aatactggtg tctgatcgcc acgagcaatt
     1021 ttttcttgat taaatgcatc tccagcctct ttgattagtt ctacttcttc taagacttgt
     1081 tcatataagg aattttcagt tagaggatga ttttcgggaa gcagtccatc ttcatccaat
     1141 tctactaatc gttcatcctg ataattttcc ggacgataaa actctacacg attatggtag
     1201 atatcataaa gaccttctag ccctttaccc ataccgattg gccaattcat cggataagat
     1261 tcgatatcta atagctcttc taattcttct aacaattcca atggttcacg accatcacga
     1321 tctagtttgt tgataaaagt aaatatcggg atgccacgtt tttttacaac ttggaataat
     1381 ttctttgtct gtgcttcgat cccttttgca ctatcgatta ccatgaccgc actatccacg
     1441 gccatcaaag tacgataagt atcttcagaa aaatcttcat gtcctggtgt atccaagata
     1501 tttactcgtt tcccttgata atcaaactgc ataacagaac tagtaacaga aatcccacgt
     1561 tgtttctcga tttccatcca gtctgatttg gcaaaattac ctgttttttt gccttttacg
     1621 gtcccggctt gacggatagc cccaccaaac aatagtaatt gttctgtaat cgttgttttc
     1681 ccagcatccg gatgggaaat gatcgcaaaa gttctgcgat tgtccacttg ttgctttaat
     1741 tctggattat tcataaccta actccatttc ttccattcaa ttatttatat tttctctagg
     1801 cacagattgt ccgatctctt aaagaaagac aatttcatat agggctgtga caaaagccgt
     1861 gtagcagtct ctttttccga gtaaacggcg ttctagagac agcctatcag caataagccc
     1921 acccaagcca aaaatacgaa aagctgtttt tgcttggctg ctcttattac ttaatgcagg
     1981 atagcttttt cacaaccttt gtcaactact acaactgaat cagtatagca tggattcatc
     2041 gaatgaaaaa gccaccaaac cataagagtc aaatattttt ttgtcagctg actaaaagaa
     2101 aagacgtgtt gcttgtttat tcaagcaaca cgtctttcat aaccaagttt ccaattatct
     2161 tcttggctcg tcatcttcga attcttttct gaagtatctt cgttcttctt caggttccgc
     2221 ttctgtttct tccggatcat gaaaaatgac acggatttta aggattcgag aaccttccat
     2281 ttcttcagaa accagcgtaa tattatcgac atcaaacgat aacttttcgc cttcatccgg
     2341 aatcatgcct aatgctgtaa tcaaataacc agccatcgta tctacatcac tcatatgcag
     2401 atcactgtca aaagcttcat tgaactcatc aatcagcata cgtccttgga tgatatattc
     2461 gtgttcatcg attttttcat aaagattctc aacttcatct gattcatcat cgatttctcc
     2521 gacgatttct tctaaaaggt cttctagtgt tgctaaccca acgacgccgc catattcatc
     2581 taataaaata gccatctgat tctgcgtctt tttcatttca tataacagat catcaataaa
     2641 gaccgtttca ggaacgaata aaggctcctg catgattttc ttgatatcta aattatcaaa
     2701 accgaattta tgggcagctt ttagtaaatt ctttgtatga aggattccga ctactttatc
     2761 tttgtcttcg ttataaacag ggattcttga atagttttcg gataaaactt cattcacatt
     2821 ttcttccaca gtgtcgttga tatcaatcat aaatgcatcc gttcgtggga ccatgacttc
     2881 tcgggcaact ttcgtatcaa gagaaaaaac cccttggagc atttctaatt cttcattatt
     2941 caatacgcct tcgttttcaa gcatataacg catttcatct ctggtcattt ttgaatcttc
     3001 atcatcaaaa gtcatcggcg tgatacgcgc caataaactt gtggaagcag aaagcagcca
     3061 gacgaagggc ttagctacta caccaatcaa gcgtatcagc cctgaagtga actttgcgac
     3121 ttcttctgat ttatttaaag caatacgttt agggtacaat tctccaaaaa caatcgatac
     3181 ataggtcaaa atagccagca caatgatatt tgcaatattt tttgccgctg tgccgccacc
     3241 taataccgga gctaaccgtt ccgataaagt attagctagt gaagcaccag acaaaatgtt
     3301 gaccaaagtg attcctactt ggattgtcga taggaaatta tttggattct gcaatatttt
     3361 taaaagcttt tgagatttca catctccttc atcagctttt tgctcgattc gattcttatt
     3421 gatcgaaaca acagcaattt cggaagctgc taaaaaagca ttgatcaatg tcaaaacgat
     3481 caataataaa atctgcgcta acagcgactg actctcaggg tcagcattca taatcgcatt
     3541 ctctccttat aaaattaatt tttagtttac gatagaaaaa aacgtggcac tatagccacg
     3601 ttaaaatggt atcacaaagc aattttcttt gcaataaaaa caagtgtact aaaactacct
     3661 acgaggtcgt agcaattttc ttcttgtttt cattttgctt gtcttctttg acgattttga
     3721 ttatatagga aatgatcgtc ttgcagattg cgtaaaaagg aacacctaag aagataccaa
     3781 gcaatccggc aatatttcca gctactagaa gaacgataat gatcgttaat ggatggattt
     3841 tcagtgattt cccaattaca ttaggataaa tgatattccc atccaactgt tggacgatta
     3901 gcacaaccac acaacaaagt aatgctttta ccggatcatt gaagactgtt actagaaatg
     3961 caggtgctag tcctagataa ggtcctaaat atggaattag gtttgtcaat cccgcaatga
     4021 ccccaaataa gaaagcataa tccacaccaa ttaccatata tcctaaaaat gtgaaggtac
     4081 caacgaataa acattcgatt gcttgtccgc taatgtaatt agccaatgtt ttattcagtt
     4141 gccccaataa ctccacaatt tggtttcttc ttttttctgg gaaaaactgt tggatgtttg
     4201 gcactagttt ttctccgtct ttcaacatat agaacaagat gaatggtgct gtaatgatta
     4261 ctacggttgc cgaagcgatc gttccaacga tcgaacctaa actattggat actccactaa
     4321 ggaattgctg aatgattgtc ccgtaagaaa tgtctaattg ctcaaaatat ttattgatat
     4381 ctacttcctt gaacaaaggc aattcggcta cctcttttaa ccagctttct acctgtttga
     4441 tgaaatcagg catattagat gccaatgagg aaatttgctg tactagactt gggatgacac
     4501 taagtaaaat ccaaataatt gctgcaacta aaaggagcag cacaattgcg acagcccata
     4561 ttcttttgat ttttgctttc atcaacagct tgacaatagg attcaataaa tagtaaagaa
     4621 agcctgcaat cagtacaggt gcaaataacg tggaaaaaaa tgtcccgatc ggttcgaaaa
     4681 taaagtttat ttctgatgaa atcatgatca atgcagctat gatcaataat tcaagcgacc
     4741 aaaacattag tttagactgt ctaagttttt caaacaatag atttcctcct ttctttctta
     4801 ctagtatata ttatattcgt taaaaattct tttattcttt ttttattttt gaaaacttgc
     4861 catacttttt atttgtttta ttttccagta tactatacga aaggaaggtg gctagaccat
     4921 gaaagttgta caaacaaaag atacaatgag caatatttac ttagatgctg tacggatcag
     4981 gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct cgagaaatag acaaagatga
     5041 agcccattgt atccacttcg tcctgtactc agataaaaaa gaaccgcaag gaactgtacg
     5101 tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg atggcgattc tttctgaata
     5161 cagacaccaa gggttaggaa aaatactgat tgaagaagca gaaaactttg ctaagaatca
     5221 aggctataat acgattttat taggggcaca gtccacagct gaaacttttt acgaaaaact
     5281 aggatataca gcttacggcg atccttttga agatgctggc atgcctcata tttatatgaa
     5341 aaagacttta taaaaattta tttcgaaaaa agcaaaaaac agcaagcttt tgagaaaaat
     5401 ctctcactgg cttactgttc ttttttgtgt ttacttacgc gacaattgct tgtaacggtt
     5461 ataccaaata tcaatatagc cttccgagaa cgggccatct ccattattga tccagtcaac
     5521 cagaatcttc acattttctt ttaggatgaa gtcaatatcg ttcggataat tcatcatttt
     5581 gctgtgcatc tcatattcat cgacatccaa tagtcgtttc tcgccgtcag gaaatacctt
     5641 gatatccaag tcatagtcga tatatttcaa ggcctcatcg tccagcaaaa aaggtgaagc
     5701 tagattacaa taataggaaa cccccttttc tctgatcatt gctattacat tgaaccagta
     5761 ttttttatga aaataaacaa tcgctggttc gcgcgttacc catcggcgtc catcagattc
     5821 agtgactaat gtgtgatcat tcacgccgat catagagtgt tcgcttgttt ttaataccat
     5881 ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg taactttgga tcgtcacaaa
     5941 ctctccttct tttggaactc ccatagcaac ccttccttaa gcagcatttt gtgctttgtg
     6001 tttgggtgtt cattacaccg acatcataat tattatatca ataaaaatga gctttgccta
     6061 gaaaggattc ctattgtttc gatttttctc taactgatct accagtttca tttggacctt
     6121 aggaaaaacg accgaatcaa atgcatcagg cttcaaccac ttcgaagttt tgttctcggt
     6181 aaattcttgt tctgctccct cggttgcccg tccgtaaaaa aggagaacat gccatttcag
     6241 atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc tgccaaacaa agagttcctg
     6301 cttttcaaat gggagatttt gctcttcttc tgctactagg tcatcaaaca gatcgatttc
     6361 tggctttgtt tcccattctt ttttcaatcg ctcatactcc tcttgtgtca cttccatcat
     6421 tggaaatgtc cacatattcg ccagcaattt ttggctatct ctttcttcaa aataatacgc
     6481 tccagaatga ttttgaagag ccgcactgat ataataaacg tcttttggct ttgctttttt
     6541 tgtttttaca ggaaaactcg tttggattcc tcgtttgtta gctaaacaaa aggcttggat
     6601 cggacaagtc tcacactttg gagaagttgg tgtacagata gctgaaccta gatccatcat
     6661 tgcttggttg aattcacctg gatgcttttc atcaatgatt ttccgcatcg cttcatcaaa
     6721 gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg caaaacaatc tgctgactac
     6781 tcgcatcacg ttgccgtcaa ccgcgggttc tggaagtcca aatgcgatac ttgcaatcgc
     6841 gcctgtcgta tatggtccta ttcccttcaa tgagctaatt tcttcaggcg tttgcggcat
     6901 ttccccatca aattctgaca tgatctgttt agcagcagct tggatgttcc gggctcttga
     6961 gtaataacca aggccctccc atgcttttaa aagcttttct tctggagcat ttgctagctc
     7021 ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa tcgatcactg tgtctactct
     7081 ggtttgctga agcatgatct cagagatcca gatacgataa ggatcacgat tatagcgcca
     7141 aggcaggttt cttttttctt gttcatacca ttgaatgaac tgatcttgaa attcttttgt
     7201 ttctttatct gtccattttt cccattcttt attcactttc ctcttccttt tccgtaatga
     7261 atcgctggat catatccaaa tcaaatcctt tttgatataa gctttgcttt acttttagat
     7321 ttcttttttt aggttcaaac cttaaatttc ttcgccatag tttatctcct tgtttgacta
     7381 aataatcata ttctatttct tgatctgctt cttgcggaag atcagccatc gcttgttgga
     7441 tcacttcttg agaaaaacct ttcgtcatca atgtttgttg gatttttctc aataattctt
     7501 tttggctttt tccgcgattt tttccaaacg ctttttcggc tgtctgatgt gcattttgaa
     7561 cttggtcttc aaatgtatat tggctgagcg cttgttcaat gatttcaggt ttcaaacctt
     7621 tttgctgcat ctgctgcgct aatttctttg gtcctttgtc acttaagcgc atattcgtgc
     7681 gcacataact ttccccatag accaaatcat cgacaaggtc caattctttt aagcgggcta
     7741 cgattttatt tcgatcttct tgaaggattt ccttttcttt taagtatgtt cggatttctt
     7801 tttccgatcg caactgataa cttaagtaat tcattgctag gtgaaaaccg aaatcagctc
     7861 cgctactctt cttgatctct tggatagttt cttcgtctaa ttcccgtcct tttaataatc
     7921 gatagcgtac taatacatct tcagatactt gtaaagcttc tccttcagat aaatctacgc
     7981 gatagaaagg gcctttgcct ttactgattc tcgtaacggt taacataaac atcctcctcg
     8041 aacatatatt ctctatcata ccacagaaaa tccccgtaat caggtattat atgttagaat
     8101 aaaccacaga aaaaagaaaa agggagatac ttatggcaga aacaattgtc aaaactggac
     8161 aaacggttcc tttgaaaatc aagcgtttag gcataaatgg tgaaggaatc ggttatttca
     8221 aacggttgat catatttgtt ccatatgcac taccaaaaga agaagtacta gtgaaaatca
     8281 cgaaagcaac tcctcgctat gcagaaggag aattaataaa agtcaaaaaa acaagcaaag
     8341 atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg tggctgtcaa ttacaacact
     8401 tagcttatca agctcaattg gattttaaaa aggatctgct tttgcaagca ttggaaaaat
     8461 tcaaaccagc aggctttcga tcttatcaac ttttgccgac gatcgggatg gatgagcctt
     8521 ggcattatag aaacaaagca caatttcagt tacgaaaaaa taagcagact caaaaaattg
     8581 aagctggttt atacgaagca aactctcatc agttagtccc tataacagac tgcttggttc
     8641 aagaacctcg aacacaaaaa gtaatgaata ctgttgttca actattgaat aaatttgatg
     8701 cacccatcta tgatgaacgg acaaacagtg gaatcttccg cacaatcatg gttagaatcg
     8761 gtgttaaaac tggggagtta caagttgttt ttattactcg cagtaaaaaa ttcccgcaaa
     8821 aaaacgcaat gatccgtgag atcaatgaac gactgcccga agttgtttca atcatgcaaa
     8881 atgtccaggc acaaaaaacg tctctcgtga tgggggatga aacgatccac ttatggggaa
     8941 aagaagccat tgaagaacaa atcaatgaag tgactttcga tctttctcct cgtgccttct
     9001 ttcaactcaa tcctcagcag accgaattgc tttatggcga aggaatcaaa gcattagatg
     9061 tccagcctaa tgaaacgatc gtggatgctt attgcggagt tgggacgatc ggcttaagtg
     9121 tagcaaaaca agccaaagaa gtacggggaa tggataccgt tcctcaagca atcgctgatg
     9181 caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg atatgaagtc gggacagcag
     9241 agcaagttct gcctaaatgg ttgaatgaag gttttcagcc tgatggtatc atcgtagatc
     9301 caccacgtac gggtttggat aacgcattga tccaatcatt attgaaatac cctccaaaaa
     9361 aacttgttta tatctcctgt aatgtttcta cacttgccag agatctggtt aatttaagta
     9421 aaaagttcaa gattgaatat ttacagtctg tcgacatgtt tcctcagact gcacgttgtg
     9481 aagttgtcgt gaagatgacg agactcgctg actaaatgaa agaggttgtg aaaaagtctg
     9541 ccctgtatca agtaataaga acagccaaac aaaaatagct cttcatgttt tatgaatacc
     9601 gtacttgtga ccgaagagtt aacaggagga cactatttat ttaaaaaaag ggttgtgaca
     9661 tagtttttgt cacagccctt tttgattatt attggatatt cacgtctatc acttgataaa
     9721 atgcatttgt cgtatcgctg atattccata ctgctaaaat cacattatat ccttttcgat
     9781 cctgcggaag tgtgatcgtt tggttgactg tttttggtgg aacagttgct ttgtcgtcta
     9841 tttttgtgat caattcaaaa tcactaaatt tcaaaggttg attaggattc caagttggtt
     9901 tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc tgttaaattc caagtaatat
     9961 ttaatgttcc tggattaacc atgtttttat gccatctgtt agatgtctgt tcatctaaag
    10021 gctcaaagcc gctgactcct gcactagcaa tttttccatc aatgaatgta tttttggtag
    10081 cttcgatact ttgcggttcc cattgtgctc ttccgacatt ttgatttaag tttcctgctg
    10141 ctgatgttcc taggtatgca cggcttcctg gagaagtaat ataaccatgc gcaaaacctg
    10201 gaatgctaaa accgaacaac cctaaactaa ctaacccaat aaacaataac gtactaatct
    10261 ttttcatgta gaaacctcct tcatttgata accacattgt aaacgcttta ttgtattaac
    10321 acaaacatta tgcttttata attttatgtt gaagtaaaaa aggtgaaaaa agtaagatgc
    10381 agattataac agaagtagac gagaaaagta caaatagaaa acatgagaac caaatcatat
    10441 gatctttttc aaaaacatca ttcatataaa caaatgttca actttttaat aacaacaatc
    10501 atcaattttt ttcttacttc acaaactaaa tacaggtctt gctgtgagcc ttcatggtaa
    10561 actactatta tagaaaggag ttagtaaaat gaagatgata aaagatattc gtttttgggt
    10621 ttgtgttata ggaattgtca tattaggctt tttatcaggg ttgcttagcg gaaatcccgg
    10681 agaatattac tattctttgc aacttccacc atttgcacca cctagctgga tatttgggcc
    10741 gatgtggacc ttactgtata tattgatggg tatttctctt tatttgttac ttaatcacaa
    10801 caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg attcagttca tttttaattt
    10861 tatatggtct gctctatttt ttaatttaag aaatattttc attgcagcaa tcgatattac
    10921 acttttagtt attttcttgt cagtgcttat gtatcagttg tggctacatc atcgcctggc
    10981 aatgtggctc atgattcctt attatttatg ggtactattc gcaactctat tgaattattc
    11041 aatttatttt ttaaactaag cgctaaaaag agacgtcctg taatttaaca catagaaaaa
    11101 gtgccaccta agttagattt tttgtctaat ttaggtggca ctctttatta accaaactat
    11161 ttttttctag tacccacttg ttgttccata ctcattattc tggtcataac cataaccatt
    11221 ttgttcctgt ccagcgtaag gatcataact ttgagtatct tgacttggtg tatagtactg
    11281 acttgtatct tctgtttgct gataataggt attcggatct tggtactgat catatgtgtt
    11341 ttctgaatag gaagaataat cggtgtaccc ttcactattc gatccatcac cgtttgtcga
    11401 atcgccatct tccgaatcat caaagaacat cattgaagca ttctcatttt tcaaattagg
    11461 taatgtatcg cttgttttta tatttaattg tttcttcagt tcattttgga tcgaaagaag
    11521 atcatttttt cctaaaattt gatagtagat atcattgatc atttgtcctt cgccttgcaa
    11581 ctggtcttgt ttgattgttt caaacgcagg ggtgtagttc gtcgctacat caagcatgtc
    11641 atcccaatct aagtccgtcg tcacattgtt ctcaactgct tttaaaattt ttctataatt
    11701 gcttacgcca tctaaactta aaactttatt gacgattttc gtcactactt cccgctgacg
    11761 acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc attcgagaat aggctaaacc
    11821 tttttctcca ttcaatgttt gttcacctac tggtacatgg acaccatcca aagtaaagtc
    11881 aattttatta ttcactttga tgccaccgac cgcattgatc aaatcttcca tgccatccaa
    11941 attgatcgtt acataatgat caataggaat atcgagcagt tgctcaattg aatccatggc
    12001 catttcgaca ccaccaaatg cataggcatg atttaattta tctaccgttc cataacctac
    12061 gatattcgta taaatatctc ggtccaaact gatgatcgtt gatttttttt gtttcggatt
    12121 aactgttact accatcatcg tatcagaacg tccttgttcc gttcttccta atccaccggt
    12181 atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca tcaatgctta ctctctcaga
    12241 acgtttggag gatattcgat tagagttttt agaaattttg tttactgttt ggctcgcttc
    12301 tcccataaat ctgattccgt atgctgataa tccggcaatc agcaaaacaa atacccctaa
    12361 caaggttaat agaacttttt gccatcgctt cataatacac ctctgtttca cattttctaa
    12421 aatattgtct catataactg ttcaatcagc aaattcactt tttaatcata agaaaacttt
    12481 aaaaaacatt tttgtgtaac gattactatt ttttaaaatt tctcaaaatt cttaactatc
    12541 ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc agttcgcaat cgttttatct
    12601 ttttttacgt attttttcca gaaaaagcga acaacaaggt attttccaaa taatcgtgca
    12661 gtaaaatgat cgcacatacc tttcccatct ttttaacagt ctttgcctac cgttcagatt
    12721 atcattcgat ttcacgaaaa tttcttccta tgactggtcc ttttgttaaa cccatcttct
    12781 attcgtttta tttccagctc actgcatggc tcttaaaatg actcgaacta gtgatttcat
    12841 taccatcatc ttttaatgga tagatatgct cctcttcaaa gtaaaacgat tttcttttaa
    12901 agtaatagaa ttgacatggt aatgagtatg tatactagtg gtttcacaaa tggtctttta
    12961 ttcaacatcc aaaaactgaa atttctttaa aatccattac taacgaagaa aatgttcctt
    13021 gcttgataat tagttaggac atgtcaagtg aacaaatgca tttacttagc caaaaaatct
    13081 aaaaacttca taaatagaca aaaagacacg actctctaag aagtcgtgtc tttattcaac
    13141 aaattattat aatttagtca cttcagcagc ttgaggtccg cgtgcacctt caacaatcgt
    13201 gaattctact tcttgacctt cttctaggct cttgaagccg tctccttgaa ttgctgagaa
    13261 atgaacaaat acgtcatctc cgccgtctac tgtaataaac ccaaatcctt tttcattgtt
    13321 gaaccattta accgttccgt gttccataaa tgtatagcct ccaaaaaagt tcttgcaagt
    13381 aaccttgcaa gaacattata cccaatttag aaaacgctag caagctttcg ctcttttatt
    13441 ttccaaatta tttttaagac tgatatgacg gaggaaatgg ttattttgct gcttcgaaac
    13501 gtttgtttac ttcatcccaa ttaataacat tccagaatgc tgcaatataa tctggtcgga
    13561 catttttata tttcaaataa taagcatgtt cccaaacgtc caaacctaga attggtgtct
    13621 ttccttccat caatggagaa tcttgatttg cagtagaggt aatagctaat tttccatttt
    13681 ccattacaag ccaagcccat ccagaaccaa atcgtccagc ggctgctttt ttgaattctt
    13741 ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc ttcttttatt tctcctgtag
    13801 gttcgccacc agcatttggt gccatgattt cccagaaaaa tgaatggtta gcatgtccgc
    13861 caccattgtt acgtacagct gtcttgatat ctgttggaat agcgtccata tcagataata
    13921 attcttctat tgttttttcg cctaattctg ggtatttctc aattgctgca tttaaattcg
    13981 tcacataagt attgtgatgc ttatcatgat gcagatgcat cgtttcttcg tcaatataag
    14041 gttccaatgc gtcatatgca taaggtagat ctggtaatgt gtaagtcata aaaaaattcc
    14101 tcctcaatgt tttctttata atgtctacat tacaaagagt aacaaagaga aggaaattct
    14161 tcaattgtta tgcctagtcg tctttttcac tttgacctac ttcttcttca aaaacctcag
    14221 tttctttaaa aataaacaac ttgctgaaaa tatagttcgc taggataaca ataatttgag
    14281 tgatgatttt cgccacatac tcattgctgt tcataacatt gatcaacaat accattgccc
    14341 ccatgtccaa tccgtaagaa aggatacggt agaagaagaa cttaccgcat tcaataagtg
    14401 catgtgtaaa attcggtgat tttgaatgga atacccaaag tttgttcgtt acaaaagcaa
    14461 acaatacaga acaaaaccac gaaatagaat tggataaggc catacttaaa tcaaaatatt
    14521 ggcgtgcgag agcaaaacta acaaaattta caatcgtagc caaaccacca aaaaagagat
    14581 agactagtat ctcccaatat ccttttgctt ctaaatatcc ttttaactgt cgatactgct
    14641 tcatgagcca acctacctta ttaaaaattc ttatttatca tactctatat ttcaataact
    14701 ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt gcctttgtct ctgcttggat
    14761 cgtcacatgt gtaataccat attttaattt taataaagaa attatacgag gatatatctt
    14821 ttcaatctcg cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa taattatctt
    14881 ttcgctatac tgccaaatat ggacatgatg aacattcttt accccatcga ttgcttcaat
    14941 ttcttgttcc attttttcat agtcaatagc aggtgcactt tgtatcaaaa tagcggcagc
    15001 ttttttgatc accattatcg cttctatcaa tatatatatc gaaatgatca gtgtaataac
    15061 tggatcgatc cagtagatat cgaaaaaacg gatcaaaatt gctccgataa tcacacttac
    15121 cgaagacagt gtatcactta acaaatgcaa ataactagat tttatattca aactttcatc
    15181 tgcttcttgt cgtaataata atactgagaa caaattcgcg agcaatccaa taactgctac
    15241 tgttagcatc aaatgactat ttatcttttc tggtgaagtg aaccgcttga atgcttcaac
    15301 ggttaaaaac aaggataata tgatcaatat gaatgagttg atgaaagcac tcaatatttc
    15361 taaacgctgg tagccatacg tacgtttttt atttgcttct tttcgggcca ttttttgtgc
    15421 cccgtaagaa aaaataattg ccaatgtatc actaaggtta tggaaagcat ctgaaacaag
    15481 agacaaactc cctgacataa tccctccaat aaattctgat aaagtgatca ccgcattcaa
    15541 aaccactact ccaaatagtt tttttgatga tctctgtggc tgtgtaggca ctattccttc
    15601 ctccatttta tttatattgg atagtcttta gacaagaata acattttttt gatatttcac
    15661 caatcattca gatgtttccc taaagcactt gtatttcact tgatacttcg ctagaattgc
    15721 aagtgatgac actactagat gaggagtact tatgacaaca aaacaattaa ttatcagttc
    15781 ctttatacga ttcaaagacc taaaggagac gagaaatgtc ccattttgga aagccattgt
    15841 ctatctttta gttttgagta taattatggc tttgccaatc tcttttcaag tttttcaagt
    15901 cttaaacaac attcaattgg atgggcaaaa gattgctact cgcattcctg attttacaat
    15961 ccaaaacggg cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa caaactccat
    16021 tatcttcaca tttgatccag agggaaaacg aacagaacag gacatctcta ctgatttgat
    16081 ggggaatttt gttagtgtcg ggatgttaaa aaacaaactt atcatagctc ttcctaatac
    16141 aggcacgact tctgctttgt tgaacaataa ccaattagaa cttccctaca ccaatgaatc
    16201 tttaaaaaac ttaacaggta aggagctccg ttctttttta agtaaagcat ctataccact
    16261 ttggataaaa gtcctgacat ttatattttc tatttatcct agttttctga atctgctgat
    16321 cactttgcta tttaccaaca ttgctgcttt tctctatgca cgctttcgac taacaaaagc
    16381 tacttttctt gattgtttaa aaacattgat ctattcaatt tcattgccta ctataattgc
    16441 aacaatcttg atggtctttc ttccttcttt tgacacaagc gccttcattg cttttgcagg
    16501 aatctttatt tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca attaagaaat
    16561 aaagacaact cgttatttct acttcaatag acccttttag aggttgtgac atttttttgt
    16621 cacagcccct tttttatttg aaaaatattt ctttctcaaa aaacaagcaa ggcgcttcaa
    16681 agttcttctt gtatgcgcta cacttagagt aaagaaaggc gggaaattaa ctatgtcaga
    16741 aagaatcatt tatgcagatc gtccatttcg tgatgaattt atcgaagaaa tccatactat
    16801 agcgcctggt tatatcttta aaacagaatt acaaccagag gatctacaaa atgtagagat
    16861 cagtttgggc tggaactcag actaccaaga aaaactatta gcctcttcta ccttgaaatg
    16921 ggtccaatct atctcagctg gtgttgatca gctacccctt gagacttttt caaagaaaca
    16981 tattttactt tcaaatggta gtggcatcca cagtgaatcg attacggagc atgtcttagg
    17041 aatcatcctt gggtattctc gtggtctttt tcaagcacaa aaagcacaga gtgacatgaa
    17101 atggttaggt tcatcggtcc attaccaatc tatccaagga aaaaaaatac taatcattgg
    17161 aactggccat attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca tgaactgcta
    17221 tggaatcaat acatccggtc atccagcaga aggaatggaa aagacttttt ctttaaatca
    17281 actaaaggaa gttcttcctg aagtagattt cgtagtcaat atcttacctc taacggagga
    17341 aacaactggg ctctttgatc agcatatgtt tgaacacttt gatccaaaat ctgtattcat
    17401 caatgttggc cgtggtgctt ctgttaagac acaagatctg gtgcaggctt taaataacca
    17461 gcaactttcc tttgcagcat tagacgtttt tgaggaagag cctcttccac aagatcatcc
    17521 tctttggaaa atggacaatg tactgattac ttcccacatt gcaggtttga cacctgattt
    17581 tcaaaagaaa ttaatggcta tttttttaac caatctgaaa agctactttg ccacccatga
    17641 gcttcaaacc aatcaagtaa aattgtctgc aggatattaa tagctgaaaa caatattttc
    17701 aaaagaacaa agagaaggtg acaaaacttg tgtcaccctc tcttttttta ttgtttttta
    17761 aagtgatagg ttggctcctc caggttggga tcataatcaa caagtaaatc atatccttta
    17821 aagaaccatt catctgcttc ctcgataaaa aaaagtgtcc catccatttc cactttcttt
    17881 acaggatgtt ctggctgttc aactgacatt ccaatcgaaa aaccatcatg aacctctgtt
    17941 ttgccgtaga cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc aatttctgag
    18001 tcaaaccact tttgtgcttt tggcgttacc gttaattcca tcccaacaac ctactttctc
    18061 ttttctttct attgattcat cttatcgtct agaatatcca catcgcctgt ctctccaatc
    18121 accagtagat tatcattttc gcgaacatat tcatctgctg caggagaagc gataaccgtt
    18181 tgatctactc ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa atttaattca
    18241 gctaatgttt tgttgaaaaa tttaggattg gagactcgga tttctgctaa agaatattta
    18301 tctgacagct ctaagtagtc tagaatattc tttgatacta agttatgtgc aatccgtatc
    18361 cccatgtctc gctcagggtg tacgacacga tctgctccga tcttctctag tacacgggca
    18421 tggtactcat tttgggcttt tgctaaaata ttcggtacgc ccatttcctt taccatcaga
    18481 gtaactaaga tactggcttg gatatcttct ccaatagcga caattacatg atcgaaattt
    18541 cttataccta atgaacgaag tgtcatctca tcctgtgcat ttgccacaac agcatgagtg
    18601 gctatattca tatattcatt gactcgatct tcattactgt caatagccag tacttcttgt
    18661 ccagcttcga tcagtgtacg gcagatgctt ccgccaaatc gtccaaggcc tacaattgca
    18721 aaattttgtt tcaccatttt cctcctttag tatcttcttt tctcagtata acaaagcctt
    18781 tccaaaaaca aaagcaagca tcattattgt aaaaaagcca agaaaaataa tagttatttt
    18841 ctcagctttt gattatcctc tttatcatcg tttttatttc accaaaccat gtttaaacgc
    18901 atagatagct gcttgagttc ggtcttctac ctctaactta gctaatatat tcgacacatg
    18961 cgttttaact gttttcagtg tgataaacaa ttcatccgcg atttcctgat tacttttccc
    19021 ttcagaaatc agcatcaaaa tttcattttc tcgattcgtt aattcttcat gcaatactgg
    19081 atcatttcgc cgactcattt tttccatcat ttttgttgtc acttcaggtt ccaatactcg
    19141 ctcgccgcgt tgcgtagccc ttatagcatc agctatttca tgtgcggtag acgtcttcaa
    19201 caaatatcct gctgctcctg cttcaattgc aggataaact ttttcatcat caataaaact
    19261 tgtgacgata atgattttgg cttttggaca atcttttaag attgcttttg ttgattcgat
    19321 gccatccatt tcttccatga ctagatccat taaaatgaca tctggtcgaa gtgccatcgc
    19381 cttttcatag ccttgacgtc cattttcagc ttctccaatc acttcaatgt cttcttggat
    19441 agataaataa gaagagacgc ctaagcgtac catttcatgg tcatctacta ataaaacttt
    19501 tatcatcttt atctatgcct ccttcatcaa agggaccttg atctccacgc tcgtaccttg
    19561 ccctttaaaa ctaatgattt ttactgttcc accaatacca gctactcgtt ctttgatatt
    19621 gttcaatcca taacttcccg ttttggtttc attcatatta aatcctgtcc cgtcgtcaat
    19681 gatacgtaat aaaagattgt tatctatttt atgcaaatag acttctaatt cattagcctt
    19741 agcatgtctt aatgtgtttg ataacaattc ttgaacgatt cggaacaagt gatcctcaat
    19801 ggaactagtt aattttacat cttctacatc ccattttagc gaaatctgga ttttattctg
    19861 caattccttc aataactgct caatgccttg ttttaaactt ttttcttcta aattgactgg
    19921 acgcagatga agaagcagcg cacgcatctc agactgggaa gcgttgatga tctctgccac
    19981 cattttcaac tgcttacgga acatttctgg tgtctcactt ttttctgctt gctccgtcaa
    20041 tgccgacatc atcatcatcg ctgcaaataa ttgctgtgaa actgaatcat gaagctcacg
    20101 agctaatcga tgcctttcta attctaaaat ctcttctttg gtttgtccat ctacatattg
    20161 aggacggcta gtcataatct gtaattcact ggatacttcg atcatttttt cttttatttt
    20221 agcaatttcc atatcaatat ctttgatgta cagatcatcc gacgcgcttg ggatcggctg
    20281 atgcaaaagt ttggattcat aattgccaga agaaagtgca cgaagctttt cttcgatttt
    20341 cccgtatttt gccttttgaa taagtgataa caatagaaat acgatcaatc ctacacctaa
    20401 tgaaattgcc atcaaatgga agataagcgg aacgtataat aaacgcgcac gcagcaattc
    20461 tccccaccaa tgactttggt tactggcata aaaataagtg aaaagtgaaa atagaataat
    20521 aaggaaagcc gcaatccccg aataaacagc tagcattgct ttggatattc ttcccatcat
    20581 attcgtatca cctcgacatc tccaaccaag gtattggtaa tgatcttcaa tctgcgagga
    20641 ttttcatcat aatcactgct gcagattttg atctgttcgt tttttaagga aaactgctct
    20701 tcttcaaaca agacatttcc tactaatgtc gcatgctcta aacgaatagc cactcccagg
    20761 ggtactaaga tccgtgttct gcctatccct tttcttacaa tcacaatatt atcatctttt
    20821 ggcaaaagtg tattacccaa atcgatgata gtatcaccag aaatgatggc aatgttgata
    20881 tcatcccatt catagacgtc gtttccaata cgttggttac caaataattg ctgtctttta
    20941 cgctcattat tatgtgtttt taattgctct gtttggacca tcatgatctg tttttttcgc
    21001 caaaaagcat tttttgtcaa atctattcct gaaatctcta cacctttcag cccgataaat
    21061 aaaacggcca atacaaccat catccataaa gcaggactgt tgactaagct aaaaaaaatg
    21121 accaagctcc ctaagatcaa ttgaaaattt tgaaatttag tacgaggata tttgcgcata
    21181 gctagatata tattgaaaat accgaatatt actaaaagca aaagtccagt attgtttacg
    21241 atttgccaaa cagcaaataa cagcagcagt gcttcgacta ccacaaaaaa acgccaagaa
    21301 ctattcatcg ctttttctcc ctcctgacac attctcattt catcatacct atttttttga
    21361 ctgttggcta taggacttta gatgtaagat cgttcagtga aacctgcaat actcaaagag
    21421 aaaagatcta gagtgcgtca tcagtaagat gaactctcta gatctttcag tcagactacg
    21481 cttgttctac tttgacgatt ttaactaaca tatcgccacc tggtgtagca atggttactt
    21541 catcattttt acgtttgcca atcaatgctt gcgcgatagg agaatcattg gaaatctttc
    21601 cagaaaacgg gtctgcttca gcacttccga caatcgtgta ctcttcttca tctccatcag
    21661 gcaactcgat gaacgtaact gttttaccga tcgatacctc atctgcatct acgccgtcat
    21721 tatcgatgat ttgtgcaaaa cggatcatat tttcaattgt tgtgattctt ccttcaacaa
    21781 atgcttgttc atctttagct gattcgtatt cggagttttc ggaaagatct ccgaaaccgc
    21841 gagcgatttt gattcgttca atgatttctc cgcgtttaac tgtttttagt tcttctaatt
    21901 ctttttctaa tttttcttta ccttctagag tcattggata aactttctct accatgtgta
    21961 acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat atagtccaaa
    22021 aaagattacc acgcttttct taaaatgtaa atgtttttct aaaaatgatc aatgactagc
    22081 ccctgttttt ctaagaatta tttacatatt tttgcactag ttccatatgt tcttcatacg
    22141 ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa tagtcatttt
    22201 ctgctggttc taatactgcc ttgattgcct tttcgcttgg attgtcaaaa ggaccaggcc
    22261 catagccggt attggtatac aaattgtatg gtgaatctac agctgtatct tcatacgtca
    22321 ccaattcttt atgttctcct aatgcatata aaacagagat atctgactga agtggcatat
    22381 ttgccttgat tcggttaaag aagacttgtg caatattttt acgatcattt tcttttacgc
    22441 cttctttttc aactagagaa gccaaagtta aaacttcttg tacagtcaaa tttttctgtt
    22501 tgatagctga ataatagttt tgcatgaccg tatttgtctt gttcaccatc tcaatcacaa
    22561 gatccttaag cgaagtactc gtgtagtaat cataagtagc tgggaataag tagccttcca
    22621 aacggtattt tgtgttctca gctttagagg cactttccaa caattctggg aatttttgat
    22681 acaactcgtt aaagaaagtc tcatcattta ctaaatctaa aaattctttc ttgtctttac
    22741 ctgttacctt cgctacccgc tcagcaatct gatcgatgtc gtagccttct ggaatggcaa
    22801 ttttcccatc tgcaactttt gttggctcag atgtgccgcc ttcttgcaac tgcttgccaa
    22861 tctcatcaag agtcatatta ggcgctaact gataataacc agcttgaaag ccagttaagt
    22921 ttttaaattt tgtataataa ttgaaaacaa tcccactttt aataatattg tctttctcca
    22981 aaatttcgcc aatctgctta ttagaagagc cgtttgggat ttccacttgg actaattggt
    23041 cgtccgattt atctaaaggc ttcaacccgc tatctacata acgatatacg gtaaacccga
    23101 gaaatccgcc aataatcaat aatgtaagag cgatcactaa aatgatcttc ctcacgatct
    23161 tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta gctgccttac
    23221 tggcttcttt tttgtctttg cttgggtgtt gtttgtttcc tgacaaaaca actcctcctt
    23281 actatcatct tcgtaactct gtttattata catgaaattt ctactgccgt gaattatttt
    23341 accaaatatt atgtcaaaat tgtaagtggc aaatgctctt tttgaggatt acaatgaaaa
    23401 atagttaaaa atggatagaa aggatttaat atggcaaaag gaaaacatca aaaaaaacag
    23461 cagatctctc tttcttttct cgcagttttg ttgctggctg tcagcttctt ttcaacaggc
    23521 atcattacag gatggcaatt aacaaaacaa cctcaaccac aaatttataa aacggaaccc
    23581 gcacaacttg cttccaattt aacatctaat gagcagatgc ttgccagcaa aattgatcaa
    23641 ttactgcaaa aatcagatta tatcggttcg atttatgtac gcaaaaataa tcgagtgata
    23701 ttagaaaaag gatatgggta cgcaaatcta cgcataaaag aatcgaattc tccttctctt
    23761 tactatcaaa tcggttcgat ccaaaaagcg atgactgctt tacttatcct aaaacaaatt
    23821 caatctggca agataacttt tgacacaaag ctatctgaat tttatcctca gattcctgga
    23881 agtcagcaaa tctcaatcaa aaatatgctt tacatgcgct ctggtcttca tcgaaccgct
    23941 tctccaaaaa aacctatgtc agatagtgag atcattcagt ttgcccttca tcatttgaaa
    24001 tttactggtt atgatacgta tcattatgaa ccattaaatt atacattgct tacaggggta
    24061 ttgatcaaac taatgaatca gccttatgaa actcttttaa aaaaagaaat cattcggcca
    24121 ctccatctag aacatacaga tttttatgaa aatgtaaaaa attcccctca gcatgctgta
    24181 tcttaccaaa tgtctgcaac tgacgattat tcaaaagcat tggtcgagcc tgaaacagac
    24241 atccgaaatg aattaggcac aggaaatata agtatgtctg tttatgactt gaacaaattc
    24301 tttaccagtg ttctgtctgg cgatattata ccaaaagaac tacttttctc attgtggcaa
    24361 aataatggtg ataaacatcc ttacagcggc ggtgtttaca gtggaaatga ttatatcctt
    24421 gctcagggaa atatcaatcg attccatgca gtagcggcga tgaaaaaaga cacaaaagat
    24481 gcaatcgtca tggaaagtaa tatccaatct gataaaaaca tcaaattgcc ggccactgat
    24541 ttaagaaatc agatttacga actgatggaa ggtgttaatt taaaaagcta agtcgagaca
    24601 aaaaaaccag caggatgctg gttttttgtt tcaatcgatt tctgtcttta aataacgttc
    24661 cattccgttc aatatatcaa atttgggttg ataacctaaa gaacggatct ttgaaatatc
    24721 cgctaaagaa tcacgaatat cccctgaacg ctccgcttca tatttaagcg ctagctctgt
    24781 tcctaagatt tgatcaattg aatgaatcaa ttcaagtagc gtagtgcttt ttcctgtacc
    24841 gacattgaat tgctgtccta aagctttttc ttccttagca acaagtaaaa gtgcttgtat
    24901 aacatcttct atataaacga agtctctaga ttggctacca tcaccaaata aagtaaattc
    24961 tgttttttct cctgccaatt gttttttgta tcggtccact agaatcgaaa taactccgct
    25021 atatggagag ttaggatttt gatttggacc ataaacgtta aaaaatcgga cagcactagt
    25081 cggaacatca tataaatgac agtaatccaa aacatattgt tctgatgcaa acttatcgat
    25141 ggcatatggt gttaaaggac ggatgaccga ttcttctttt tttggcaacg ttggttcatc
    25201 gccataaact gcagctgatg aactaaatac caatcgtttt aaaccagatt gatattttcg
    25261 taccaattca agcaataaaa gcgcactgtc aaaatttaca cgatgagttt ctagaggacg
    25321 ttctacagaa tctgccacac tagctacagc agctaaatga aaaatatagg caaactgata
    25381 ctgtttcatg attctttcca tcaattgagg atctgataca tccccctcaa tgaaagtcac
    25441 ttgtttctcc atatccaagt tttctttttt tcccattgat aaatcgtcga ctactacgat
    25501 tttgttttct tttcccaaat aattcgctag cgttgaaccg ataaaaccag ctccaccagt
    25561 aatcaatata gtatccatca aattcactcc aattatgcat tctcatactt aataaagtta
    25621 gtttaccatt tttatattgt aaaaactata aaatttgttg aagttgggat aactagtttt
    25681 ctt
//
LOCUS       DAZKNO010000020        25496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000020 DAZKNO010000000
VERSION     DAZKNO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.020"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..560)
                     /locus_tag="ACG8MY_000618"
     CDS             complement(195..560)
                     /locus_tag="ACG8MY_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429844.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            768..1250
                     /locus_tag="ACG8MY_000619"
     CDS             768..1250
                     /locus_tag="ACG8MY_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429845.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1447..2337
                     /locus_tag="ACG8MY_000620"
     CDS             1447..2337
                     /locus_tag="ACG8MY_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0429846.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2663..5137
                     /locus_tag="ACG8MY_000621"
     CDS             2663..5137
                     /locus_tag="ACG8MY_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0429847.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5240..6976)
                     /locus_tag="ACG8MY_000622"
     CDS             complement(5240..6976)
                     /locus_tag="ACG8MY_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0429848.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6973..7677)
                     /locus_tag="ACG8MY_000623"
     CDS             complement(6973..7677)
                     /locus_tag="ACG8MY_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0429849.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7808..8311
                     /locus_tag="ACG8MY_000624"
     CDS             7808..8311
                     /locus_tag="ACG8MY_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0429850.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8271..8609
                     /locus_tag="ACG8MY_000625"
     CDS             8271..8609
                     /locus_tag="ACG8MY_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0429851.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8630..10048
                     /gene="ffh"
                     /locus_tag="ACG8MY_000626"
     CDS             8630..10048
                     /gene="ffh"
                     /locus_tag="ACG8MY_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0429852.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10160..10738
                     /locus_tag="ACG8MY_000627"
     CDS             10160..10738
                     /locus_tag="ACG8MY_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0429853.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10742..11263
                     /locus_tag="ACG8MY_000628"
     CDS             10742..11263
                     /locus_tag="ACG8MY_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0429854.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11342..11896)
                     /locus_tag="ACG8MY_000629"
     CDS             complement(11342..11896)
                     /locus_tag="ACG8MY_000629"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0429855.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12149..12424
                     /gene="rpsP"
                     /locus_tag="ACG8MY_000630"
     CDS             12149..12424
                     /gene="rpsP"
                     /locus_tag="ACG8MY_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0429856.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12436..12687
                     /locus_tag="ACG8MY_000631"
     CDS             12436..12687
                     /locus_tag="ACG8MY_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0429857.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12811..13602
                     /locus_tag="ACG8MY_000632"
     CDS             12811..13602
                     /locus_tag="ACG8MY_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429858.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13772..14293
                     /gene="rimM"
                     /locus_tag="ACG8MY_000633"
     CDS             13772..14293
                     /gene="rimM"
                     /locus_tag="ACG8MY_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0429859.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14283..15044
                     /gene="trmD"
                     /locus_tag="ACG8MY_000634"
     CDS             14283..15044
                     /gene="trmD"
                     /locus_tag="ACG8MY_000634"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0429860.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15180..15527
                     /gene="rplS"
                     /locus_tag="ACG8MY_000635"
     CDS             15180..15527
                     /gene="rplS"
                     /locus_tag="ACG8MY_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0429861.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15837..16079
                     /locus_tag="ACG8MY_000636"
     CDS             15837..16079
                     /locus_tag="ACG8MY_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0429862.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16167..16313)
                     /locus_tag="ACG8MY_000637"
                     /pseudo
     CDS             complement(16167..16313)
                     /locus_tag="ACG8MY_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            16370..16684
                     /locus_tag="ACG8MY_000638"
                     /pseudo
     CDS             16370..16684
                     /locus_tag="ACG8MY_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            16713..17972
                     /locus_tag="ACG8MY_000639"
     CDS             16713..17972
                     /locus_tag="ACG8MY_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0429863.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18025..18921
                     /locus_tag="ACG8MY_000640"
     CDS             18025..18921
                     /locus_tag="ACG8MY_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0429864.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            18932..19801
                     /locus_tag="ACG8MY_000641"
     CDS             18932..19801
                     /locus_tag="ACG8MY_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0429865.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            19811..20486
                     /locus_tag="ACG8MY_000642"
                     /pseudo
     CDS             19811..20486
                     /locus_tag="ACG8MY_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            20692..22356
                     /gene="alsS"
                     /locus_tag="ACG8MY_000643"
     CDS             20692..22356
                     /gene="alsS"
                     /locus_tag="ACG8MY_000643"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0429866.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22369..23079
                     /gene="budA"
                     /locus_tag="ACG8MY_000644"
     CDS             22369..23079
                     /gene="budA"
                     /locus_tag="ACG8MY_000644"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0429867.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23209..23706
                     /locus_tag="ACG8MY_000645"
     CDS             23209..23706
                     /locus_tag="ACG8MY_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0429868.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            23891..24151
                     /locus_tag="ACG8MY_000646"
     CDS             23891..24151
                     /locus_tag="ACG8MY_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429869.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(24513..24752)
                     /locus_tag="ACG8MY_000647"
     CDS             complement(24513..24752)
                     /locus_tag="ACG8MY_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429870.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            25038..25352
                     /locus_tag="ACG8MY_000648"
     CDS             25038..25352
                     /locus_tag="ACG8MY_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429871.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         8462 a         4314 c         5238 g         7482 t
ORIGIN      
        1 atgtttccac ctcaaattca atatctatta ttggaatcat tattgctgca attattacac
       61 aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa atagaaaaac tagtgatagt
      121 tttggtgata taagaaaaac atatatgtat aaaaagaaga aagtagcttc tctttgtaca
      181 tatatggttt gtttttatgg atgccgagct tttttataag ataaggctac cgcccataac
      241 gacccaagga tttgtggaac taagtaaata cctgcaagaa gcaagatcag ctgggtcggt
      301 tttaaaggat gatagaagta acgccattca gtaaaattgg cgcaaaataa ataggaagtg
      361 acagtagata taatattggc aatgaataaa gatataatac gcctgttcct ttgaaaataa
      421 tatccagcaa aaagagataa aaatatcaat gtgatgaacc cgcttacgga atgataacga
      481 aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag ataaaagcaa cggaaggttt
      541 ttccagcaag tttttatcat ctttcatcac cacctaacaa gtgttattat tttgttagtt
      601 ttattgtaaa ttgaatcctg caataatcaa ttactttttc attatttcta gattaaatga
      661 ataaactctc atgccattat ctctaaaaaa aaagtttttc cccctaattt ttttagatag
      721 aaaattgtat agtatgatga gagaaaaagt agtttggggg aacgttaatg agaagaaaag
      781 cagtaatcga tcggctttat ttattttttt ggttttttcc tatcgttttg ctacctttta
      841 catgggtaaa tgctgttgta caaggtgcgg agatattagg cctgatccaa ggaagcggat
      901 tagtggtttc taggaatttt ccagctttga ctgttttcta tttagtgata agtgtaggtc
      961 tcttattacc aaaatctctg atcaatcact acaattggca gctgctttac ttttcttgct
     1021 tattagtatt tacatctatg ttttctttgc tgcctcgctt gattatcggc cctaatggtt
     1081 tgagcgagag tggattgaag gattcagctt attcctccat gtatttcttg acgatcaaac
     1141 catggttcta tttagtaatt gcctcattta ttcttgcaac atttttttac ttctggacag
     1201 aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa taactattga tggtggatca
     1261 tcagtaaaaa agcatatgag ctgcgtaaaa tgcatgtttc tcatatgctt tttttattat
     1321 tatgtaaaga atgcgaaaaa cgtttacatg atttttacat aacttaacta tatttttaca
     1381 gcaaccatac gagggattta cagtcgactg ataaacttta gttgtaaata agaaaggagc
     1441 aataaaatga aaaaagtatt gtttactcta ggtatgatgg gaattctttt agccggatgc
     1501 ggctcaggat caggaaatac agatggctct gccacacaaa ataattcaag tgactcaaat
     1561 gaacaagtaa aaatcgttgc ggtaggttcg acagcattac agccgttagt cgatgcagca
     1621 caagaaagct ttgtacaaga aaatccaaat tatcaaatct ctgtacaagg aggaggaagc
     1681 ggaactggtt tgagccaagt cgaagctgga gcagttacta tcggaaactc tgatgtattt
     1741 gcagaagaaa gagacggagt tgatgcttca aaattagtgg atcataaagt agcagtggtc
     1801 gggatggcac caatcgtcaa taaagataca gatgtcaaag atatcaccaa acaggaattg
     1861 attgatatct ttacaggaaa aatcactaac tggaaggaag ttggcgggaa agatcaaaaa
     1921 atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg cgacattcga aaaatgggga
     1981 ctggatggtg ctacccctgt gcagtcccaa gaacaagatt catcaggaac agttcggcag
     2041 ctggtaagtc aaacaccagg agcaatcagc tatttagctt tctcatatct cgatgattcc
     2101 acacaagcac taagtatcga cggtgtagaa ccaaaagaag agaatgtagc agataacagc
     2161 tggggaatct ggtcatatga acatatgtac acgaacggaa aaccttcccc tgaagtccaa
     2221 aaattcttag actacatgat gacagaagaa atccaagaag gacctgtcaa agaattgggc
     2281 tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg aaggaaatat caaataacta
     2341 gcagataaac atgccacggt agtcgttact attaaggaaa aatcaactat gtttccccac
     2401 atatacattt ttcctttaat tgtgaaaaat agttcctcat attttgccgt tttttgagct
     2461 aataccgagg gtcagctgat tgaacaccgt ttattcgaaa aaaagagcta cgacatagaa
     2521 ctgtcgtagc tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg
     2581 aggaacactt tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa
     2641 cgaaatcaat cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa
     2701 agtaacacaa agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt
     2761 aacggcagat caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc
     2821 cacattcaaa acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat
     2881 ctttcttatc ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt
     2941 accagttatc atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat
     3001 cttggacgaa ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga
     3061 acaaacaata gatatagaag accttgtaat agatgacatt gttattttag agactggaca
     3121 tcaaattcca gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt
     3181 gacaggcgaa gcagatgaaa taaccaaaga aataggcgat ccattaatgt ccggcagttt
     3241 cattgtttcc ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc
     3301 ccagctgaca gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc
     3361 tcttaatcaa ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt
     3421 ttctcaaagt tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc
     3481 agcattgatc ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt
     3541 aagtgcgact aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac
     3601 attggcaagg gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat
     3661 gtctgttcaa aaagtgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt
     3721 ggaagcatgc attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc
     3781 tgttaaagca tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc
     3841 ttctgtccaa aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc
     3901 cgaaatggtt ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga
     3961 aaaaggctat cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt
     4021 gacagaagca gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc
     4081 aagagccaca tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga
     4141 taatccgctc accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta
     4201 cgttgatgca tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt
     4261 ttttggacgc gtcaagccag aacaaaagaa aatcttcgtt caattgctaa agcgtcgtaa
     4321 caatacagta gctatgacag gtgatggcgt gaatgatatt ctagccatga aagaagcaga
     4381 ctgcagtata gcgatggctt ccggaaatga agccgccatg caggcctcac aagtggtctt
     4441 acttgattct gacttttcac gcatgccaga agttgttgca gaaggacggc gcgttgtgaa
     4501 caatattgag cgatcagcca gcttgttttt agtgaagaat attttctcct ttttgctctc
     4561 actgttttca gtgatctttg ctctgaccta tcctttagaa ccatcacaaa tcacattgat
     4621 tagtttgttt actatcggac ttccatcttt cttgcttgct ctagaagaaa acaaaaagcg
     4681 aatcagaggg aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg gactaacaga
     4741 tatgatcgta gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa ataaaacaga
     4801 tgtttctaca gcatctacca tgcttttgat tgcagtaggg ttcctcgttc tctataaaat
     4861 ctgttcaccg ctgaataaat tcagaagtca gatcattctt ttctgtgcta gcggtaactt
     4921 tttttcggtc atctttttgc ataagctgtt ttcaatcacc ggtatctcag ctgtatcact
     4981 tttgctgttg agcattctat ttttctcggc agattcgatt tttaggcatt tgaccacatt
     5041 ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta gtcgcgtagt
     5101 taaaaaattt ctatcgatat ttaaacgaga aaattgacga aaagtatctc aaaaaagagt
     5161 aaaaaagaac aaccactcgt gtatctttcg aagcaggatg agtcatccaa ctttaaaacg
     5221 cacgagcagt tgctcttttt tacattttcg gaatggtaac ggtgaaagtc gaacctgttc
     5281 caagatgact gtctacagta atcgtaccat gaaggagttg cacgtagtct tttacaatcg
     5341 ctaaccctaa tccggtcccg cctgaatgcc ggcttcgcgc cttgtctacg cggtaaaaac
     5401 gctcaaagat ccgttcctga tcctcaatgt cgatacctat accaaagtct tggacagaaa
     5461 acgatagatc atctgttgcc tgatagcgga taataatctc gctgtcgctt tttgaatatt
     5521 ggacagcatt ttcaatcaga tttttgataa ttggataaag gatatctgtt ttagtagtaa
     5581 atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg attggctgat
     5641 aattttgaac gatttgctgg aaataaggtt ctgtataaat tgtctggatc tcgtaagaat
     5701 tctcgccatc ttttgacagc tggatgattt cccgaatcag tttatctaac cggatcgcat
     5761 ctttttgcat gatctcaaga aaagaagtca gagtttgcgg gtcatctttt gcaccatcca
     5821 gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt ttttaattcg tgagagacgt
     5881 tgccaacgaa atccttttgt agcttttcta aacgtcgcac tttcgttaag tcatacgcca
     5941 cccctagaat ctgtccggta ccttctgaat tagtaaagta gcgcaaactc aagtctaatg
     6001 tctgccgacc ttctgtaagg gtgatctcct gatggatcag cggatgctca ggagtcactt
     6061 gatgaatcag ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc tgttcaggtt
     6121 gatagcttac cacgtttaaa tgtagctgca tctttggatt aagcagtagt aaacgagaat
     6181 cagcatcatc aatcaggaat acgccaatca tcagttcgtt cagtaacgta tataattgct
     6241 cttctgtggt agtatacgcc cgatacatct tactcatttg ttcgctcaac gcattgatcg
     6301 tctgatacaa ttcttcccat tgatcgggtg tttgcatgat tacttccgcc tgattaggat
     6361 tttgcaccat tttttttagt acgggcagca cggttttcaa tggctggttt ttttgataga
     6421 tcagataata gatcattcct gcaagaacaa ggaagaaaga cagaaagaag aaaaagaccc
     6481 aacggcgaaa actctcagtt ctcggtaaaa aaccgcttgt cggttctgcg atacgtaaaa
     6541 tggcttctaa ctggccactt tttttgactg gtaatgcgac atataaaaga tccccattca
     6601 aagtagtact tttgcgcaaa gcgctaccta ggttcccacc attcaatacg gctttgattt
     6661 cgggacggga gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat aagatctttc
     6721 cagtagcatc cattaaagta atccgttcgt tcgactgatg gacgaattct tccaaagcag
     6781 ttttgttttg cgctgcttgg aggttttcga catctaattg ccggatcagc agttgtgctt
     6841 tttttgtcag gtagctttct tgctgttcca cgatctgctg ttgaaaataa tgggaaatca
     6901 attgccagcc accaaaaaac aaactaacta aaagaataag aaagaagaga cgttcataaa
     6961 tttttttcca tttcatcgtt tgggttcctg aaacttatag ccaaatcctc taatagtcac
     7021 caagtacttt gggtgttttg gatctcgctc gattttttct cgcaaatgac taacgtgtac
     7081 atctacgatt cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta gcaatgtgtc
     7141 tcggtcgatc acacggtctt ttcgtttcat gaaataaaca agtaattcaa attctttcgg
     7201 tgttagttca atcagatggt cgtctacggt cacttgataa ttggtcaaat cagcagtgat
     7261 ttgtccaatc gatagatatt caggctcagc ttcatctttt gattgcttac gaggttcaat
     7321 tcgtctgaat attgctttca tacgggctaa tacttcgcga ggactaaatg gtttggtcaa
     7381 atagtcgtca gcacctattt ctaggccaat gatccgatcc acttggtcat ctttagccgt
     7441 cagcatcaaa atcggtgttt catttttttc ttgacgcagc cgctgagtga tctccatgcc
     7501 atccatcgtt ggaagcatca catctaaaat gatgaaatca aatgattgtt ctaatgctaa
     7561 ttctaatcct tcgcctccat ctgtggcact ggttaccttg tagccttctt tttccaaatt
     7621 gaacgtcaag agcgttacaa tagaaggttc atcatcaaca actagtattt ttttcattca
     7681 aatctccttt aaataggttt cattctgttt atattttagc atagaatcag tacaaagggc
     7741 tattttacaa aagaaataaa tggtataata ggaaggcact ttgctgaata gaaaggaaga
     7801 gcagtcaatg attggaatca gtgcatgttt aggcggtctg gcctgtcgct atgatggaca
     7861 gaaaaaagaa gtaccagaat tgatggaact agttgaaact ggagaagctg tcatggtctg
     7921 tccagaagtg atcggcggat taccaattcc tagagatccc gctgaaatca ttggtggaaa
     7981 tggattcgat gtgtggaaag atcaagcaaa agtatggacg acaagcggcg aagatgtaac
     8041 ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa aacacattga
     8101 tctagtcatc atgaaggaaa aaagcccttc atgcggaacc cattcgattt atgacgggag
     8161 tttttccgga ataaaaaaaa atggtgccgg agtggctact gcttatttta ttcagcaaaa
     8221 aatgactgtg ctctctgatg aagaatggct gaaaactaga ggtgagcaga atggaaatcg
     8281 aaaagaccaa tcgaatgaac gcgctctttg aattttattc gaccttattg acagaaaaac
     8341 aaatgaacta catggaatta tattatgcag atgatttttc attaggtgag atcgcggaag
     8401 aatacgaagt aagccgacaa gcagtatatg acaatatcaa gcggacaagc aagatcttgg
     8461 aggattatga aaaaaaactt catctttttt ctaattatat tgttcgagaa caggtattgg
     8521 aaaatatgac tgcatatatt acagataagt acccggaaga taaaaaacta cttgaatatg
     8581 tacagcaaat tcaagcaatc gaagaataaa taaaaggaat gaagaaaata tggcatttga
     8641 aagcttaact gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag gaaaagtatc
     8701 agaagctgac gtaaaagaaa tgatgcggga aatccgcctt gccttactag aagccgatgt
     8761 taacttgcaa gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg taggggcaga
     8821 agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg aagaattgac
     8881 gatcacactt ggttctgaaa cagcagagtt aaataaatca ccaaaaatac caactgttat
     8941 catgatggct ggtcttcaag gggcaggtaa aacgaccttt actggtaaat tagccaatta
     9001 tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat atcgaccagc
     9061 tgcgatcgac caattgaaag tattaggtca acagctagac gttcctgtat ttgatatggg
     9121 aactgaagtc agcccggtag agatcgtccg tcaagggatg gagttagcaa aagaaaagaa
     9181 aaacgactat gtattgatcg atacggcagg acgtctgcat atcgatgaaa cattgatgga
     9241 tgagttgaaa caaatcaaag aattgacgca accaaatgag attttgcttg ttgtcgatgc
     9301 catgactggt caagatgccg tcaacgtagc tgatagcttc aaccagcaat taggtattac
     9361 aggggtcgtc atcactaaac ttgatgggga tactcgaggc ggggcagcac tatcgatccg
     9421 ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt gaaaaattga ctgacttaga
     9481 agtattccac ccagatcgta tggctagccg tatccttggt atgggggata tgctgacatt
     9541 gattgaaaaa gcgcagcaag actatgacga gaaaaaagcg gaagaacttg cacagaaaat
     9601 gcgggaaaat tcattcgatt tcaatgactt tattgaacag cttgatcaag tgatgggtat
     9661 gggcccactg gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc ctggaatcga
     9721 aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa gcgatggttt attctatgac
     9781 gccagcagaa agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc gaattgctgc
     9841 cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca aggaatcaag
     9901 aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg accaaatgtt
     9961 tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga tcaagaaaaa
    10021 taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta cagactgaaa
    10081 atgtctactg ccggagcttt tctatcagca atttttattc tttactaata caattactct
    10141 aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa gtatgcggca
    10201 atatcatgat actgtctggg gtgttcctga atatgatgac caaaagctgt tccgaaaatt
    10261 gatgcttgat attaatcaag ccggattgag ctggcagacg attttaaata aaagtgaagc
    10321 attcgatgaa gcatatgatc gattcgatat taaaaaagtc gcaaattatg gagaaacaaa
    10381 aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa tcgaagcagc
    10441 cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt tttcagagta
    10501 tctttggtcg ttttctaatg gaaaaatcgt tgattcttct tatcattcac aagaagaagt
    10561 cccaacaaca agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa gaggatttaa
    10621 atttatcgga agcacaacaa tctatgcttt tttagaagct gtcgggatca tcaatgatca
    10681 ccttgacagt tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg gcgaataaag
    10741 catgcgaaaa atcatatttt atggtgctat aagtttagat ggctatttgg caacgaaaac
    10801 ggatgatctt caatggcttt ttgacactcc aacaggtaag caaaccactt atgaagcttt
    10861 ttatgaaaca atcgatacga ccatcatggg cagaaaaact tatcaggaag caaaaaaata
    10921 tttggagacc gaagcaattt atccagagaa aaagaatttc gttttttcca cccacgcaca
    10981 attacaattg ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc taaaaaatct
    11041 acaaatgaca gaaggaaaag atatctgggt tgtgggtggc ggtcgattga tcaaaccgct
    11101 attggaacat caaatgattg atgaatggta tatccagatt acccctgtat tattagggga
    11161 cgggattcct ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc tagacacgac
    11221 acgattcggt gaatttatcg aattgcatta tgtacgaaag tagaagtaaa taaaataaaa
    11281 aaggcaacta tatgcagaaa tgtcctgtac atagttgcct tttttattac cgttatgctt
    11341 tttactctgt taatttgtta gaaaaatcta gatacgcatc taaaatcgtt tggaagaaac
    11401 ttaatgttcc ttcaacgata ttaccatttt catcaattag attcgcaacg tttccgatat
    11461 atgcttccgg ttgctgcaag gttggcacat tcaaaaagac aagtgattgt cgtagatggt
    11521 ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt aacgactaaa cctggtttat
    11581 gatcccaaac actgtgtcca taaggtcttg agcctacatc taaagcattt ttcaatactg
    11641 ctggcacaga acggttgtat tcaggagtaa cgaaaacgac accgtctagt cccctcactt
    11701 cctcacgaaa acgagtccat tcagcaggag cctgttctgg agtttcgaga tcctcattgt
    11761 aaaatggcag atcatcgatt tttacaaata cactttcgta cccttcagga agcaatttcg
    11821 caaaagcttc tgctactttt ttgttgtaag aatcttttct taaactacca ataaaaaatc
    11881 caattttttt tgtcatacga tttttcctcc ttcaaactat ctataacaat cttacaaaaa
    11941 ataagtaact ggagcaaata aaaacactta ctgacaaaag ctacatagaa gatagattac
    12001 ttatttttaa caaaaaagaa gtctaaaatc ttctatttcc taaaaaaaat cgaatttaat
    12061 caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact acatcttgtg
    12121 aataaaacaa tggaggtgga aataaataat ggcagttaaa atccgtttaa aacgtatggg
    12181 ttctaaaaag agtccgtttt accgtatcgt cgtagctgat tcacgttctc ctcgtgatgg
    12241 acgtttcatc gaaactgtag gtacttacaa tcctttgaaa gaccctgcag aagtagtttt
    12301 aaaagaagac ttagttcttg actggttatc aaaaggtgca caaccttctg acactgtacg
    12361 taacatcctt tcaaaagaag gcgttatgca aaaacaccac gaagctaaat tctcaaagaa
    12421 ataaggtgac cggatatgaa agatttaagc gacttagtct taacaatcgt tcgtccgcta
    12481 gtcacttatc ctgatcaagt caagttagac gtcgtagagt cgaaagactt ttacgagtac
    12541 aatctaacag tggctcctga agatattggc cgtatcattg gaaaacaagg gcgtgttgct
    12601 aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa agtgcgtttg
    12661 aatatcatcg atgataagga aaagtaaata aacgtgctgt actcgaattt tcgagtgcag
    12721 cttttttgtt tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt
    12781 atccttatag gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt
    12841 gctctattgt taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca
    12901 agcagtatat ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggctaccacc
    12961 actgaacaaa caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa
    13021 gaacaacagc caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat
    13081 atcgcagcga cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc
    13141 ctcgggttga atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa
    13201 tatgggtttg cttctagtca agaaacaatc caagttttgc agccctttga gatcgacaca
    13261 aatggtcagc aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt
    13321 tcaagttttc ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata
    13381 gaaggccaag atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt
    13441 ctaccagcac ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga
    13501 gccgtagaag taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct
    13561 atgtctgcgt tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg
    13621 agcccatcat ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc
    13681 ataattttgg cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat
    13741 aaaacagaca aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat
    13801 cgtaaacaca caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga
    13861 agaacgttac aaaaaaggaa cggtgttaac actattccaa gacggaaaag cacctgtgga
    13921 attaacagtc aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca
    13981 tccttctatc aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa
    14041 ctctgtagat ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt
    14101 agaagatgga actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt
    14161 ttgggtcgtt caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt
    14221 caaagaagtc gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga
    14281 cgatgaagat tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat
    14341 ccatcatcgg taaggctgta gataaaggat tattagatat caatatctct aacttccgcg
    14401 attattcaga taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta
    14461 tgctgctcaa agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg
    14521 atgtcaaaaa aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg
    14581 cagaagaatt ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg
    14641 atgaacgtat ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg
    14701 ggggagaatt aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt
    14761 tagggaatca gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc
    14821 aatatactcg cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa
    14881 atcataaatt gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc
    14941 ggcctgatct tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa
    15001 tcaagaaaga agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatatt
    15061 ctttacagta ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat
    15121 tacgatattc cgctatgaga aaagctcata agaatatttg gaataaggag aagaaaacca
    15181 tgaatccatt aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc
    15241 gccctggtga cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc
    15301 agttatttga aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag
    15361 tacgtaaagt ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg
    15421 ttgcaaaaat cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac
    15481 gtgcattaca cggaaaagct gctcgtatca aagaaatccg tcgttaatca ttgttatcag
    15541 actgagaaag aacccttggt attcaagggt tcttttttct tagcatgtaa tttgaacacc
    15601 ctgatccagt ttttttcttt aattattggt aggggagaat aatggattag aataaatcta
    15661 cctataagta gtagtaaaaa aaagagcact atgataatat ctttcttaag agtaaccgct
    15721 ttaataaaaa atccagaata ttctaacata aatggagtat tcttagaatc aaaaggagaa
    15781 agaagatggt ttatacttat acggagcaag aaaaaatgaa agtttgagac acattcatga
    15841 atgatggaaa agtaaaacaa gtaccaagtt caacaaaaaa gaaaaacatc ctattgaaag
    15901 aagtcttgaa acgctttgac catggagtga cttatacaga aacagaggtt aattcgatac
    15961 tgctgaacgt attttctagt ggcgattatg tcgaacaaag acgttatcta atcacatttg
    16021 gctttttcaa aagaagttca gacggtcggg catatcagat gatgggaatt gaaaattaga
    16081 agttttaatt tatactcatt gaaaaaagtt caagtatgcg atattggttc tacaccgcat
    16141 acttgaactt tttttgtcat ctgtatctat aatacaaagc caagaacgtt attcaaaata
    16201 tggatagcta tgataaaagc taatgagcga ttcgcagtta aataagcaat cgtcagtaaa
    16261 atcgttccag gagcgtacat gaaaaaatca attggatgcg ttacatgaat aaagataatt
    16321 ctcttttatt tgtacgaata aataccaaaa cgtacctcct aaaattagat tcaaagaaga
    16381 tagcaaagag gaaaaagata gttctgcagc agctatagcg gcatgtggat tgctagaact
    16441 ttccaaacaa ctgcctgttt cagatcctcg tcatttcgat tacgaagaga tagcaatcaa
    16501 aatcttggaa gaattacaga aaaactatac aacgaaacga gttaacggat caaacggtct
    16561 gcttctccat gccgtttatg ataaaaacag cttaaaaggt gtagatgaat gtgtgatctg
    16621 gggcgattac ttttatgtag aaggaatcac tcgtctagct aaaacatggt actgttactg
    16681 gtgatgtata agagaagagg aggattcata taatgaagcg atggaaaaaa atgatgattg
    16741 gatcaatagt tttaggaatg tcactcgtat ttggtgcttg tggtaatcaa gaagacagta
    16801 gtgaagaatc agcaaatgaa attaccgttt cgacatggaa ttatgaaaca acacctgaat
    16861 tcaaagcgct ctttgaagca ttcgaaaaga aaacgggaat caaagtgaaa gcagtagata
    16921 ttgcttcaga tgattatgat acgaaactca ccacgatgct ttcttctgga gacacgacag
    16981 atgtattgac gatgaaaaat cttttgtcgt attcaaatta tgctttacga gatcagttag
    17041 tcgatcaaac agaacgaatt agagaattag acactgatgc tgctgaagaa acatatgaaa
    17101 tgtatgatat cgatggaaaa acatatgcat tgccatatcg aacggacttt tgggtactat
    17161 attacaataa gaaaatgttt gatgatgcag gaatcgaata tccaaaaaat ctaacttggg
    17221 acgaatatga agatttagca aaaaaactta gtaaaaatga cggacaagtt tacggagcat
    17281 atcaacatat ctggcgttcg acaatccaag ctattgcagc tgctcaaaac gaggcgaatc
    17341 ttgttgaacc agattatcaa tatatgactg attattatga tcgagcattg aggatgcaaa
    17401 aagaaggcgc gcagatggat tttggcacgg ctaaatcaac gaaagtaacc tatcagtctc
    17461 aatttgaaga acaaaaagca gcaatgatgt acatgggaac ttggtatatg gctggcatac
    17521 tcgcaaacaa agaagcaagt aagacagaag tagaatgggg aattacagct attcctcaga
    17581 agaaaaaagg agaatcggtc acgacgtttg gctcaccaac agcatttgct gtcaataaaa
    17641 actcaaagaa acaaaaagcg gcgcaagaat ttattgaatt tgcagccagt gaagaaggag
    17701 caaaagtatt agcaggagta ggagtagttc cttcatatcg tacggatgag attgatcagc
    17761 tttattttaa tctcgctgga atgccaacgg atgaaatttc taaaaaagct ttttctcctg
    17821 atgaaatcaa acttgaattt ccgattgata cgtatgggcc tgcaatcgat aagattttac
    17881 aagaagaata tgacttagtt ttggttggag atgaaacgcc ggaaaaagga acggtaaata
    17941 tggagaaacg tgtagctgcg gaaaaagaat aatcatgaaa ggagagaagc caagagataa
    18001 aacactcttg gtttcgtcat cgaaatggag aatagtataa ataagaaagc tacaaatcat
    18061 ttgcgtagaa aacgaacgtg gatcgcttat tcgtttatcg ccccgaattt cattggtttt
    18121 ttcgttttta cattaatacc aatcctgttt tcgcttgtac tagcatttat gaaatgggat
    18181 tctttttcaa cgccagaatt tgctggacta gataactttc gtaaaatggc caacgatgag
    18241 acattttgga tttctttgaa aaatactttt ttatataccg ctgctgtagt gcctggcaca
    18301 cttgtttgtt cgttagggct agctttacta ctaaatcaga aaattagagg gatgaagctt
    18361 tttcgtacga tatttttctt tccgtatgtt acttccttag tagcaattgc tgttgtttgg
    18421 aatatgttgt ttcatccaac agtgggacct attaatatct ttttaagacg atttattgaa
    18481 catcctccag gttggacttc aagttcaaat tgggcgcttt gggcaattgt aattgtcagt
    18541 atttggcgag ggatgggtta ttacatgatt ctataccttg caggactaca aagtatccaa
    18601 aaagaactat atgaagcgtc ttcgctagat ggagcaaacc gttggaaaca atttttgaat
    18661 gtgacattac cttcgttgag accaacaacc ttctttgtaa cgatcatgct tgtcatcaat
    18721 tgtttcaagg tgtttgacct tgtgcaagtg atgacggatg gcggccctgg tcgagcgaca
    18781 aatgtgctgg tttaccaaat ctacaatgaa gcattcaaaa aatttgattt tggttatgct
    18841 tcagctattt cacttgtttt gttcgtaatt gttttgggcg ttacggttgt tcaattttct
    18901 tggaataagc gtcgtactta ggaggtgtga tatggaagag agtgttcgaa atgaaagaaa
    18961 aaaagcaaaa attcgttcaa aaaacatgcc ctttattcaa tggttgggta tgattctgct
    19021 gattgtatta gctttattga cgatcattcc ttttttatgg atggtttctg cttcattcaa
    19081 agcaaataat gaagtgttaa cgattccgat tcaatggatc cctaaaactt ggcatttaga
    19141 gaattatctg gttatttggt ctcgtattcc tttagtgaca ttttttaaga atacagcttt
    19201 tttaagcgtt gtgatcacgt tgattcagtt gtttacttct agttttgctg tttacgggtt
    19261 ttctgaacta aattttaaag ggcgcgatat tttattttta gcatatatcg gcacgattgc
    19321 tgtcccttgg cagtcttata tgatcccgca gttcattatg atgagaaaag tgggtttaac
    19381 agatacgttg tggtcgttgg tgctattaca agcgttcaat gcttttggtg tgttcttgtt
    19441 gaagcaatat tacagtagta tcccagattc tttatgtgag agtgcgcgga tcgatggctt
    19501 atcagagtgg gaaatctatc gcaagatcat tttacctctt acaaaaccgg cgattgccag
    19561 tttgacgatc atcacattcg ttaatacatg gaacgattat atggggccat tcatttattt
    19621 gtcttctacg gaaaataaaa caatccagtt agggttgaaa atgtttattg ggctatttga
    19681 tgcagagtac gcattaatca tggctgcctc agttgtttct gttcttcctg ttgcttttat
    19741 atttttagcg atgcaaaaat attttgtaga agggatcgct tcatcaggaa tgaaggaata
    19801 ggaggtgatt atgaaagagc agcgttttga caaaaatgtt tatatggaat tcttttattg
    19861 gttgtatcaa tcattggcaa gtagcctttg tttttggtgt gtcaatatct tgtatatagc
    19921 agctgcgttc cttttgtcga tagatggaag gaatgtcttt tcctttattt tttctttact
    19981 ttttattggt ccaggttgca tcgtgatgat aggtatgaca gaagaattag caaaagaaaa
    20041 aagtttttca ccagtacgtt tgttcataaa gacgtttcgc caattttggc taaagggatt
    20101 tttttattgg ctatttgctt ggatagttag tgtgataatg atttttgatt gtttcttttt
    20161 tatacgtttt tcttatggaa aatggttgat tccgttgttt gttttacttg cttgttttac
    20221 ttgcttgttt atcagtttcc tttagcatca actgctggta ttttcaggtg agaaatccag
    20281 cgtcgaaacc aaatcaagtt ctacggatag cgttctacta tacgttgaaa aaatggtatg
    20341 tgagtttact agatttttta ctattgacaa gtttgtttct cttctttttc gtaaagcccc
    20401 aatggtgtat tctactaggt ccttctatcg tatttggttt gatttacttc aacaatcgta
    20461 aattgatgag aactatggat ctgtagactt tataaattga aactttcaat taagaatgac
    20521 agttttttat gtaaaaactg tcattctttt tcattttttg aaacatatgg ctaaacaaat
    20581 gaaataaaac atgctatact tttgaatgaa acaggtttag aaactgtatc acttatttgt
    20641 gattatttct aactaataat taaaaataaa gcataaaaag gacgatgttc aatggctgaa
    20701 aaaatgaaac aaggttcgaa atctgtagta gaaagtttga ttaatcatca tgttgattat
    20761 gtattcggta taccaggtgc aaaaatcgat ggtgtattta atgaattaga ggatcaaggt
    20821 cctgaattaa tcgtgactcg tcacgaacaa aatgctgcat ttatggcaca agcaatcgga
    20881 cgtattacag gagagccagg agtagttatc gcaacaagtg gtcctggtgc tagtaactta
    20941 gctacaggtt tggtaacagc gacagcagaa ggtgacccag ttttagcaat tgccggacaa
    21001 gtaaaacgaa gcgatctatt gaagttgacc catcaaagca tggataatgc agcactgttc
    21061 cagccaatca caaaatacag tgcagaaatc caagatccag aaacaatttc agaagtgatt
    21121 gccaatgctt atcgtatggc taagagttca aaaaaaggcg cgagctttat cagtatcccg
    21181 caagatgtag tggatgcacc tgtccaaggt aatgtcatca agccattgag cgatcctaaa
    21241 ttaggttctg cttctgcaga tgatatccgt tacctagctg aacgcattag agaagcaaaa
    21301 ctaccggtac tattagtagg tatgcgagga tcaagtgaaa aagaaacgtt agccatcagg
    21361 caattagttg aaaagacagc attgcctgtt gtagagacat tccaagcagc tggagtgatc
    21421 tcacgtgaat tagaagcgca tttctttggt cgtgtgggtt tatttagaaa ccaaccagga
    21481 gatatgcttt tgaaacgaag tgatctagtt attgcaattg gctatgatcc aatcgaatat
    21541 gaagcaagaa actggaatgc ggaaaaagat gcgcggatca ttgtgatcga tgaagcgcca
    21601 gctgaaatcg atccttttat gcagcctgaa agagaattga tcggtgatat ctcagccact
    21661 cttgatttat tgacaggttc attagagcct caacaagtgt cagaagatgc aaaagaatat
    21721 ttagcatctc ttcaagcaaa attaacggag cgggacattg ttcaatcaaa aggagaagca
    21781 ggtatcttgc atcctttaga agttattaac acgcttcagt caaaagtaac agatgatatg
    21841 actgttaccg tagatgtagg tagtcattat atttggatgg cacgccactt tagaagttat
    21901 gaacctcgtc atttgttatt cagtaatggt atgcaaacgc ttggtgttgc attgccatgg
    21961 gcgatttctg ctgcattggt gcgtccaaat acacagatcg tatcagtttc cggcgatggg
    22021 ggatttttat tctctgccca ggatttagaa acggcggtac gtaaaaaatt gaatatcgtt
    22081 catttgattt ggaatgatgg acactacaat atggtagagt tccaagaaaa aatgaaatat
    22141 cagcgggcgt caggtgtaga ctttggtcca gttgattttg tgaaatatgc agaagctttt
    22201 ggcgctaaag gaatccgagc tacaagtgta gaggaattag aaaaagcttt agaagaagga
    22261 tttgcgacag aaggacccgt aattattgat attccgattg attaccgtga taatgagaaa
    22321 ttgggagaaa caatcttacc agatcaattc tattaaggaa gtgtcgacat gactgaaaaa
    22381 atcttatacc agcacgggac attaggagct ttgatggccg gcttgatgga tggaacagaa
    22441 acgattgctc atatattaga aaaaggaact ctgggattgg gaacattgca tgggcttgat
    22501 ggtgaagtca tctttttaga tggcgtagct tatcaaggaa gatcagatgg ctcagttgcc
    22561 caattagatg gaagtgaact taccccctat gcagcaatta cagattttac gccagatact
    22621 agtttttctg tctcaaaaac tgcagatgga gaacaattga aaaaagacat attagtaaga
    22681 gaagccgggg aaaacctatt tttggcagtg aagataactg gattattcaa aaatatgcac
    22741 atccgtatca tgccgaagca agaaaaacct tacagaagac tagcaaaaat ttcagagtct
    22801 caaccagaat tccaacaaag caatgtccaa ggaacattag tcgggttctt tacgcctgaa
    22861 ttattccaag gagttgctgc agcgggattt cacctgcatt ttatcgatga tacacataca
    22921 tttggcggac atgtaatgga ttttgaagtg gctgaaggaa aagttgagat tagccgcatc
    22981 tcgtcccttg tacagcattt tccagttgat gatccgactt ttgttcacga aacgattgat
    23041 tatgctgatt tagcagcaga aatccaacaa gcagaataga aaaagacagc gagatgcgtt
    23101 caaggtgatt aacctgacgt atctcgtttt tttgaggaga aaacctagcg tttacaaaaa
    23161 aactgagcta taatcttctc catagatcaa aaaggagcgg tttttatcat gtggacagaa
    23221 aaacagaaaa aagatgcata cgaaatttta ttattacagc aacaaggatt attagaagca
    23281 gaagataact ggttagcaaa tttagcaaat tcatctgcat tgttgaatga gactttgcct
    23341 gagactgtct ttgcaggata ttatttattc gatggtaatg aattgatttt aggaccgttc
    23401 caagggcgag tttcttgcac acgtatcaag atgggaaaag gagtttgtgg agaatcagct
    23461 gaaaaacaag aaacattgat cgttgatgat gtcaaaaccc atgcaaatta catttcctgc
    23521 gattcagccg cacaatcaga aatcgtagtg ccaatggtaa aagaaggtaa tttaatcggt
    23581 gttttagata tcgattgcgg agtaacgaaa gggtatgatg aaatcgatca agcatttcta
    23641 gaaaaatatg tagatctttt attagcaaat tttaatataa atgctttttc atttattaat
    23701 aactaaaatc gtctattgaa acaaaaaaga agagaaatca agaataaaaa gagccggaaa
    23761 gacagtgggc atttctgttt ttcggttttt ttattttttt ataaaatcac aagtgttatt
    23821 gattttcata atcatatttt aatgttatta tcattttaaa taatgaaaaa acattaagga
    23881 tggatgaaat atgaataacg aaactatcat tttaggaaaa aaatatattt gccaacctat
    23941 tggcttgaaa caaaaagtga ttggagaagt aatcagtaaa ttagaaaatt gtgttgtgat
    24001 ctgcgtagac gaatatcatg acgtcgatcg caatgaaata ttagaaaaat gcggaaaagt
    24061 agtggcaaaa tatactcacg tttatgagtt ggaaacaaca gaatatctat ataactatag
    24121 caacaaatat gaacatactt tagtttcata aagatttaaa taaagtgcct tcaaaagcga
    24181 gaagaccaag cttttgaagg cactttattt agttggcagt gattgttcat gatttttgat
    24241 ttattcaaaa ggttcgccta gagaatctgt ctctgtaacg aggcctggtg gagaaactcg
    24301 ataaaaacct atactgccag aactgtccta ttttcggata gacaaactag tagttttaaa
    24361 aagatcatcc gatccaatca tttcaaaaaa tgcaaaactg atatcaggat catttagctc
    24421 aggcttgtta tcgtaaagca tttgtaaagg ttgcatttag ttaggagtaa acgcgaaact
    24481 atcatcatta tttgctgaag aagatgaaga agctaattca gagtttgttt gaaataaagg
    24541 agacgtgctg acagtttctt taccctgttt tttgcagagg tagaaacttt tatatcggaa
    24601 gtttctaccg gagaattttt ctcatttgta gaaattgttg aatcgatact attcgaaatt
    24661 tgcgtatctg aaggtatgtt ctttgatgca gaagtcccac aagcagtaag taacaataaa
    24721 tttgataaac tgataaacag aattcttttc attatgtaca cactccttat agtatcgttt
    24781 agcatacacc tatgctcagt attaggcaaa atcaatgcaa aactatcaag tcttttatag
    24841 tatctattag tcttagtaat acatatatgg agagggtgat atcccagttc aaaccagata
    24901 cattggttac catctgtatt aacgattatg cctctaaacg ctttttttat gaaaaaattt
    24961 ataagtgaaa acgtaggtat gatattatct agaggtaatt tttttgggta tttaaatagt
    25021 tgctaggagg atagcttttg gatttttaca gtaattttat cttaataata gcaatattat
    25081 tacttctaaa tatttggttt tttgataaga gtagaaatgc agggataggt tttagaacca
    25141 aacgaagtac gtcttctgaa aaaaagtggg tatattcaca gactattttt tacggagggg
    25201 ttatttcaat cagtttactt tcttcaacac tttattcttt taatgttata gacgtttcca
    25261 tgtcaaattt tatatctatt attggaatcc ttatttctgc aattattaca caactacttc
    25321 ttgtgtttga agagaagtca aaaaataatt aaagcaatta tgccttttag tacaggaatt
    25381 attacttctt acaaatgtat agcgaatgtg aagcctgctg ttttaattgt tctagaaaaa
    25441 cttttgagtg aacaattatt ttatgttagg atgaattaat ttctctagag tttata
//
LOCUS       DAZKNO010000021        24578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000021 DAZKNO010000000
VERSION     DAZKNO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.021"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(153..551)
                     /locus_tag="ACG8MY_000649"
     CDS             complement(153..551)
                     /locus_tag="ACG8MY_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0429872.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="ACG8MY_000650"
     CDS             complement(548..1321)
                     /locus_tag="ACG8MY_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0429873.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="ACG8MY_000651"
     CDS             complement(1318..2001)
                     /locus_tag="ACG8MY_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0429874.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="ACG8MY_000652"
     CDS             complement(2168..3055)
                     /locus_tag="ACG8MY_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0429875.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="ACG8MY_000653"
     CDS             complement(3052..3921)
                     /locus_tag="ACG8MY_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0429876.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="ACG8MY_000654"
     CDS             complement(4058..5344)
                     /locus_tag="ACG8MY_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0429877.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="ACG8MY_000655"
     CDS             5617..6195
                     /locus_tag="ACG8MY_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0429878.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="ACG8MY_000656"
     CDS             6195..7124
                     /locus_tag="ACG8MY_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0429879.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="ACG8MY_000657"
     CDS             7121..8857
                     /locus_tag="ACG8MY_000657"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0429880.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG8MY_000658"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="ACG8MY_000658"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0429881.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="ACG8MY_000659"
     CDS             complement(9753..10262)
                     /locus_tag="ACG8MY_000659"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0429882.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG8MY_000660"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="ACG8MY_000660"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0429883.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="ACG8MY_000661"
     CDS             complement(11563..12654)
                     /locus_tag="ACG8MY_000661"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0429884.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG8MY_000662"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="ACG8MY_000662"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0429885.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG8MY_000663"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="ACG8MY_000663"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0429886.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG8MY_000664"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="ACG8MY_000664"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0429887.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="ACG8MY_000665"
     CDS             complement(16617..17591)
                     /locus_tag="ACG8MY_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0429888.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="ACG8MY_000666"
     CDS             complement(17572..19455)
                     /locus_tag="ACG8MY_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0429889.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="ACG8MY_000667"
     CDS             complement(19473..21698)
                     /locus_tag="ACG8MY_000667"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0429890.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="ACG8MY_000668"
     CDS             21813..22847
                     /locus_tag="ACG8MY_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0429891.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="ACG8MY_000669"
     CDS             complement(22914..23927)
                     /locus_tag="ACG8MY_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0429892.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="ACG8MY_000670"
     CDS             complement(24005..24193)
                     /locus_tag="ACG8MY_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0429893.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7064 a         5341 c         4397 g         7776 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taactaaa
//
LOCUS       DAZKNO010000022        24441 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000022 DAZKNO010000000
VERSION     DAZKNO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.022"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG8MY_000671"
     CDS             <1..888
                     /locus_tag="ACG8MY_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0429894.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(1373..2002)
                     /gene="udk"
                     /locus_tag="ACG8MY_000672"
     CDS             complement(1373..2002)
                     /gene="udk"
                     /locus_tag="ACG8MY_000672"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0429895.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(2086..2781)
                     /locus_tag="ACG8MY_000673"
     CDS             complement(2086..2781)
                     /locus_tag="ACG8MY_000673"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0429896.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(2794..3510)
                     /locus_tag="ACG8MY_000674"
     CDS             complement(2794..3510)
                     /locus_tag="ACG8MY_000674"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0429897.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            3840..4445
                     /locus_tag="ACG8MY_000675"
     CDS             3840..4445
                     /locus_tag="ACG8MY_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0429898.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            4770..5093
                     /locus_tag="ACG8MY_000676"
     CDS             4770..5093
                     /locus_tag="ACG8MY_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0429899.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            5275..6645
                     /locus_tag="ACG8MY_000677"
     CDS             5275..6645
                     /locus_tag="ACG8MY_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0429900.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(6775..8076)
                     /locus_tag="ACG8MY_000678"
     CDS             complement(6775..8076)
                     /locus_tag="ACG8MY_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0429901.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(8306..10297)
                     /locus_tag="ACG8MY_000679"
     CDS             complement(8306..10297)
                     /locus_tag="ACG8MY_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0429902.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(10441..10707)
                     /locus_tag="ACG8MY_000680"
     CDS             complement(10441..10707)
                     /locus_tag="ACG8MY_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0429903.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(11217..13343)
                     /gene="pnp"
                     /locus_tag="ACG8MY_000681"
     CDS             complement(11217..13343)
                     /gene="pnp"
                     /locus_tag="ACG8MY_000681"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0429904.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(13531..13800)
                     /gene="rpsO"
                     /locus_tag="ACG8MY_000682"
     CDS             complement(13531..13800)
                     /gene="rpsO"
                     /locus_tag="ACG8MY_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0429905.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            14051..14614
                     /gene="def"
                     /locus_tag="ACG8MY_000683"
     CDS             14051..14614
                     /gene="def"
                     /locus_tag="ACG8MY_000683"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0429906.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14867..15388)
                     /locus_tag="ACG8MY_000684"
     CDS             complement(14867..15388)
                     /locus_tag="ACG8MY_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0429907.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(15391..16233)
                     /locus_tag="ACG8MY_000685"
     CDS             complement(15391..16233)
                     /locus_tag="ACG8MY_000685"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0429908.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(16226..16951)
                     /locus_tag="ACG8MY_000686"
     CDS             complement(16226..16951)
                     /locus_tag="ACG8MY_000686"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0429909.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            17127..17423
                     /locus_tag="ACG8MY_000687"
     CDS             17127..17423
                     /locus_tag="ACG8MY_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429910.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      17679..17853
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17991..19583
                     /locus_tag="ACG8MY_000688"
     CDS             17991..19583
                     /locus_tag="ACG8MY_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0429911.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            19596..20336
                     /locus_tag="ACG8MY_000689"
     CDS             19596..20336
                     /locus_tag="ACG8MY_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429912.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            20504..21853
                     /locus_tag="ACG8MY_000690"
     CDS             20504..21853
                     /locus_tag="ACG8MY_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0429913.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21981..22571)
                     /locus_tag="ACG8MY_000691"
     CDS             complement(21981..22571)
                     /locus_tag="ACG8MY_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0429914.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22718..24070
                     /gene="brnQ"
                     /locus_tag="ACG8MY_000692"
     CDS             22718..24070
                     /gene="brnQ"
                     /locus_tag="ACG8MY_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0429915.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         7121 a         4863 c         4634 g         7823 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc attagggggt gcgacaaaag agcttcttca aaaaaataag tcgaaatctc
      961 acacaaattc gagaaataat tttcgtaagt ttcttcttat ttattgcatc tgaccacttc
     1021 tgttacaacc tctcgttaaa cggtgttcaa aagcttgcat ctacgataat aagcccaacc
     1081 caacgaaaaa cgtgaggagc tgttttattt ggatgctctt atcgcttgat gcaaagacag
     1141 cttttttaca acctctactt cacggtgctc aatcagctga ccttcggtat tagctcaaaa
     1201 aacggtaaaa tataaacagc tattttccgt ttcttctttt taatacttag gtcaagatgc
     1261 tgttttcaca acctcttgtt ataaataaaa aaacagttgg ctggtgactg ttcaaaaagc
     1321 agaatccttg cttttcaaac ggtcgcagtc cgttcaactg tttcgctgcg cactatttat
     1381 ggtttaagaa actagctact tttgttgtaa tcaaatcaat agcaacgtgg ttttctccgc
     1441 cttctggaac aatgatatcc gcatagcgct tcgttggttc gataaattga tgatacatcg
     1501 gtttaacaac tgtcaaatat tgttcgatca cggagtccaa tgtgcgtccc cgttcttcca
     1561 tatcccgttt gatacgacga atgatacgaa tgtcatcatc tgtatctacg tatactttga
     1621 tatccatcaa gtcacgcaga cgttcgtctt ctaaaatcag gattccttct agaatgatca
     1681 cttcttttgg ttcctgaatg atcgttgcct gacttcttgt atgcgctaca tagtcataca
     1741 cgggtttttc aatggcttta tagttcagta agtcgcctac atgctgaatc agcagatcat
     1801 tatcaaaggc aaatgggtga tcgtaatttg tgttcaatcg ttcttcaaaa ctcaaatggc
     1861 tctgatcctt gtaataagaa tcttgttcaa gcatcatgat ggaatggtcc gggaagttgt
     1921 tgaaaatcgc acggctgaca cttgtctttc cgcttccgga tccacccgtc acaccaatga
     1981 taattggctt gctttttgtc attgactaaa acctctttcg tatccgtttt ttcattctaa
     2041 tctattatag cgaaaaaaca cgaaaatgct atatttttct gaaaattaat ccttttcaat
     2101 ttccaataat gaataaagtt cctctaatga atcgattcga taagtaggct ggatttcttc
     2161 gatttctggt aatttatttc tattgaacca aatcgtatcg atccctgcca tatttcctcc
     2221 tttgatatca gaagtcaaag aatcaccaat gatcacagtt tgctttttat caaaattagg
     2281 aatgtgggaa aagacatgat caaaaaattc tttcatcggc ttttgcgctc cgacagcttc
     2341 tgagacaaag atatccttga agtaatcaag cattcctgca tctgttaatc gccgatgttg
     2401 cgtgtgcgac acaccgttcg tcacaatata aagatcagca tgcggatgga cctttttcag
     2461 gatttctaga cttcctggaa caagatcatg accttggttc aagtagtggc ggtagcgttt
     2521 ttccatttga tcactatcta ctgttttccc aaattgctgg aaaagtaacc caaaacgact
     2581 gcctattatt tcttctcgtg atagtctgcc tttttcaaac tcctgccata aatgatgatt
     2641 caaagcctta tagctggttt cgatttctgg tgtcaatgtg attccttgtt ctgcaaacaa
     2701 agcacttaaa gcattggctt cagttaattg aaaatctaac aatgtatcat ctacatcgaa
     2761 caataaagtt ttatatttca tctttttgac ctcctattta aaataaatac ttaacttttt
     2821 catctctgct aatgtcaacg atcgatactc gcctactcct agctgttcat ctaattccaa
     2881 tggtcccatg cttagccgtt tcaatgcggt cactttcttt ccttgggcta agaacatctt
     2941 tttgacttga tggaattttc cttcttggat cgtcaataaa accgtacttt ggtgcttgtc
     3001 tgctttgagt atttttaaat cagccggctt acattgatac ccatcagcga atacgattcc
     3061 attttcaaat gctgttacgt ctgccggtgt caccctttcg ttcacaacta cttgataagt
     3121 ctttgaaacc tttttttctg gaagtagcat ttcatatccc aattgtccat tcgaagtgat
     3181 cagcaatagt cctgttgtat ctctatctag tcttcccact ggatataatg gttggaaacg
     3241 atcggcactt gcaatcagat cgattactgt cgtcttttct ggatctgata cagctgtaac
     3301 agctttttca ggtttattga gaagataata ggactcattt gtttctaagt gttcacctgc
     3361 tacttcgatt tgatgcagta aactatctac atttcgattt tgtcgaaatt cgactgtgcc
     3421 atctatcatc acttgcttca aagcaaacag ccgcttcatt tccttgcgtg tcgtttctaa
     3481 tttttcttct attaatttat ctaaacgcat gctgtttcct ttcttattac tactaattaa
     3541 ttataccttg aatattcttg cttgcaaata catcatcata gttattcctt gacttaatta
     3601 acagttcacg ttacggttaa atagtaaagt gaattggatt tctcatacac agcgtaatga
     3661 cctggtggag aaaagttgaa tcctgacttt ctaaggggag atgcgcgcat ctctcctttt
     3721 gcgctttttt aagcgccctg ccttcgatga tgtgcacgag attagccttt agaattcact
     3781 taaataaaat ctgtccgtta ccaaagcaga cagtcacatt ttaaggagga attttggtta
     3841 tgtcagtatc attagaagta agtaaaagag aagtacgtcc ccgttcatta agaaacaaac
     3901 ttcgtcacga agggaaagtt ccagctgttg tttatgggta taaagtagaa agtaccccta
     3961 tctcattcga tggtttgaca ctaagcaaat tgttgcgtga acatggagcg aacacagtca
     4021 tcacgatgga catcgatgga caaaaagtca atacattggt tcacaagatc caaacgaaca
     4081 cttttaccaa tcatttcgaa catgcagaat ttctatctgt aaacatgtcg gaagaaacag
     4141 aagtcgaaac agaagtcgtt cttgttggcg aatcagtggg tgctaaatct ggtggagtgt
     4201 tagctcaaaa cttgtataca gtagtcgtat ctgctacacc agacaaattg cctgaacgaa
     4261 ttgaagttga cgtgacaaac ttggaattag gtgattctat tacagtagcc gatcttccaa
     4321 aaaacgacga ttacaaattc gtgaccgaca gtgaagagca aatcgctgct gtagtcgctc
     4381 aaagtgaaga agaaagcaca ggtgaaggtg gcgaaccaga agtgatcggt tcaacagaag
     4441 aataatactg aaacaatcag gtggtgttag aagcggcggg tcagcagctt ttaacacgcc
     4501 ttttttattt ctaatcgttt attttcatat ggggcttcca cttttgtaaa tggctttaca
     4561 aacgaaaaat cctaatttac caagcttttc ctcgcatact tttatcgtac ctttataaaa
     4621 atttatactt tgaaaaaaag tattcaatta ttgttgcatt tttcctgatt ctttattaga
     4681 atgaaccctc gttgaagtaa gtatttctag tttatcctag gattaattat aaaaataaaa
     4741 cattcgttga ttcgaaggag gttaaacaga tgtttgaaag ttttgatagc agccgcaacc
     4801 gttacgcttc agttggtgtc gtatcaagtt tgccagatga attgattgac agtatatggt
     4861 ttattattga cctagatctt aagggagtca ttccattaga aaatatatta acctttgatt
     4921 tgatcgaaaa tcgtggaaaa gtgacgatgc atttttctca agcaggaagc aatgtggaaa
     4981 tggatattga tttgccgttt gcctattcga accaatttcc tgctcaagta tacgcctatg
     5041 atgacggtac gagagaaacg atccttttac caagtgagat tagacaatat taaggtgata
     5101 gataaacgtg gatgtccctt attttacaga ccaggccaag ttgttgattc tttgactcaa
     5161 ttcttggctt ctttttatgg agtttcataa attatcattg atttctaatt ggtattctca
     5221 ggataaacgc tatatactac taactaaatt ttaaagatat aaggagttta aaagatgcgc
     5281 gatttaacaa aaggaagccc cgctaaactg attttgatgt tcactgttcc tttactgatc
     5341 ggaaatgttt ttcagcagtt ctacaatatg atcgatatga tcatcgtggg tcagacgcta
     5401 ggtaaaaatg cgctggcagc ggttggtgca actggaagcc tgacattttt gatcattggc
     5461 tttgcccaag gtttaacagc tggtttagcc attattaccg cacaacgtta cggtgcaaaa
     5521 gattatcggg gtctgaagaa aagttttgct gctagtgttg tcattagtct tgtcgtcact
     5581 gtgattttaa ctgtcctaag tctcctgttt attcgtccga tgcttcaatt gatgcaaact
     5641 cctcctgaaa tacttgatca agcacaaaca tttatttcaa ttattttatt agggatcttt
     5701 gcatcagtca gtttcaattt gctttctaat gtcattcgcg cacttggtga cagccgcaca
     5761 ccactattct ttctgatcat tgcggtcatc atcaacgtcg tacttgattt gatctttatt
     5821 atctattttg gcatgggtgt ggaaggtgca gctattgcta cagtaatcgc acaagtttct
     5881 tccagcgtac tgtgtctagt gtatatcaag aaaaaaatgc ctttgttaca gttacgcaaa
     5941 aaagatttca aatttgaaaa agaagagttt gctgtacacc taaatgctgc tttgccaatg
     6001 gcgtttcaat cttcgatcat cgctgttggt gctatcgtgt tacaagcagc actaaatagt
     6061 ctagggacag atgtcgtagc tgctcaagcc gctgctggtc gtatcgacca atttgcaaat
     6121 cagccgatga tgtcatttgg tattgcaatg gcaactttca ctgcacaaaa ctatggtgcg
     6181 aaagaatatg gccgaatctt gcaaggggta cgtcaaactt tactgatgag tgtcggattt
     6241 agtctcttag caggtgctat cgtcattttc ttcggtcatt tctttgttca attgtttgtt
     6301 agcccatcag aaacacgtgt atttgaatta gcacaaacct acttcaatat caatgggtct
     6361 ctttactgga tcttagcagt cctgttcatt ttacgctaca cacttcaagg gcttggacaa
     6421 agcaagatcc ctacgatcgc cggtatgatg gaattgctta tgcgttcttt tgcagctatc
     6481 gttcttacag gaatgtgggg atacccagga gcagcagcag cttctccttt agcttggatc
     6541 ggttctgttg ccgtgttgct ttactcttat atgcgttcga tgaaacaatt gaaacacatg
     6601 ggtgaagagg aacatttcac attagaacat gcagaagcaa gatagccttg attaaaagaa
     6661 atcgttgcgg ctaaaaatga aaacttttat tgaaaatatc ttcttcgttt accgaaaaca
     6721 ggttttttag ataacaaaaa ggctgtgaca aaaaatgtca cagccttttt tcttttattc
     6781 tatttttctt tccgtggact ctactttgaa ataatcgata tgtgcatgct ggtattctaa
     6841 taccgtgaaa cggaatgatg caatctcaat gattgttcct gcttttattt ctttatatcg
     6901 gctagaagca aagcccgcaa gtgtagtaaa attcgtttct tcaaactctg gaatctctac
     6961 atgaaaataa cgctggaaat catctaatgt tagtttgccg gagatgatat aagagccatc
     7021 tgactgttcc gctatcaatg cttcgtccaa atctgtctca tctcggattt ccccgacgat
     7081 ttcttctaaa atatcttcaa tcgtcactaa gccgcttgtc cctccgtatt catcttttaa
     7141 gatagcaata tgctttccct tttttcgcat gatcgtcaat aattttttta tagggatcgt
     7201 ttcagttgtt acgatcggct cttcaatcaa ctgttctact gtcagctggt catctttata
     7261 agattgcttg atcaaatctg gcaaggtgac ataacctaaa acattgtctt ttgattctaa
     7321 gataaccgga tagcgagtat gtccttgttc aatcgaagct tttatcgctt ctttgacggt
     7381 ttctgaagct tcgaatactt gcatggacgt acggtctact tgtatatctt ttgccaaggt
     7441 atcatcaaat tcaaacacgt tttccaaata atgatattct tctttggtca actctccttt
     7501 ttcataagaa ttcaatgcga ctaatgtcag ctcttcttga gacaatgtct cttctccttc
     7561 agatacgaga cgcacaccta atagcttccc tagccctgca gctgaatgat tcaataccca
     7621 aataaaagga aacattactt tataaaaata atgaagtggt ttaactacga acaagacgac
     7681 cttctccgta ttgacgatac tatagctctt aggaagcagt tcgccaataa ccacatcgat
     7741 atacgtaact aaaataaatc ccagcgccac agagattgta ctgctaatag ccgaactaaa
     7801 aggtagttta ccgatcactg gttctagtaa agccgcgaca gatccttcac cgacccaccc
     7861 gatgatcaaa ccggttagcg taattcctaa ttgactagct gataaatagt tatctaaatg
     7921 gtgtgtaaca tgaagagcta gcttggcgtt ctttacattt tccttttcta gttgttccaa
     7981 tcttgatcgt ctgattttca ctaaagcaaa ttcacttgct acaaaaaatg aagtaattgc
     8041 aatcatcaaa aataaaataa tgaaattgat aaccatattc tcccgccttt ctctttttct
     8101 attgtaccct ttttctacaa aaagattgaa aataatgcgt attcatattt aggaataaaa
     8161 aaggagatgc ggcatttttc agccacatct ccttatcact tcaaaatcat tgcgttactt
     8221 gttgctaaac agctttttca tgacctctga taaacggtgt tcaagtagct gacattcgac
     8281 attagttcag gtcaaagcgc tactttcaca accgctcttt caaccatgtt ttccgagtat
     8341 ttccaatgat aagcggaaca gtttcatgtt ttacttcgct gtactctagc ccaaaccatg
     8401 tttctggcga ggtagcgatt tttttgattg ccagttttgc ctgcatgatt tgacgttgcc
     8461 attttcctag agttgtcaca ttggaaacta cttcatcaat caggacaaat tggaaatcgc
     8521 caacttctcg ccctggtgtc agagagtatc tttgtggctg ttttggcaaa cggccttgtt
     8581 tcatcaaatc atggatgact tggcgaacat atacgttgat ttcctgttgg accctgaaac
     8641 ctaagaacag attcacttgg acaataaaat cagtgtccat catatctact tgatattctt
     8701 tcgtatatgg ctcatccgtc acttccacat gaacaaacca atagactcgt gctcttttag
     8761 gccgtttatc taaaatcgaa tagatgaact gtcggccaac tttatcttca gccatatcag
     8821 gaaccaagaa gaccacattt gtctgataga ttggtaaaga agtatcatca cgtaattctc
     8881 taagctgcgg gatataatct ctcaaagaca catcctcggt tgacgattcg cgaataatgt
     8941 tcccttgttc ccaaataaac atcacgacta agatgatcaa accaatcaga acggctacaa
     9001 aaccgcctcg gaagaatttg acaacacttg agataaagaa aacgacttct atcgcaccga
     9061 agaaaaaggt aactaaatat gcaagccaac gagaatgccc attttggata agataaaagt
     9121 aaagcagtgc tgttgtcatc aacatcgtca cagtgatcga tagaccgtaa gcagcttcca
     9181 tatgatgaga cgttcggaat gtgacaacca ctagagaaca ggcgatccat aaaatcagat
     9241 tgactgctgg gatatacatt tgcccgatac tgttcccagg atacatgatc tttaaccgag
     9301 gcagcagttt caacttgatt gcttctgaaa ctagcgtaaa cgaacctgta atcaatgctt
     9361 gcgaagcaat gactgcagcg actgttgcaa aagcaacgcc gatcacgatc caactttgag
     9421 gcatcatttg gaagaatgga ttcaggttct tgatttgctg tgcttcaggg ttttgataaa
     9481 cattcaacaa ccacgcagcc tgcccaaaat aattgataac caaacaaatt ttgacgtaag
     9541 gccagctcag acgaatattt cgtttgccga catgtccaag atcggaataa agcgcctctg
     9601 cacctgtcgt agccaggaaa atatttccaa gaatgaacaa gcctaatttg ttatctgcac
     9661 taaaaagcag ctggatagca taataaggat tcagtgcacg gataactgtc cagtcttgac
     9721 tgaagttcat taatcccatt acgcctaaaa aagtaaacca agcgaacatg attggtccga
     9781 atgcttttcc gacaatatct gtaccaaagc gttggaccat gaataaaaca aaaataataa
     9841 ctaaagtgat aatgacgatc acgttctggt tattaccgaa ccggtcaaaa aactgcggaa
     9901 ttcctcgcaa gccttcaatc gcagtcgtta ccgtcacagc aggggtcaat accccatctg
     9961 ctagtaaggc agctcctccg atcattgcag gaatgatcaa atatttccca ccttttcgga
    10021 caagtgtgta gagtgagaaa attccacctt caccatggtt atctgcattc aatgcaatca
    10081 cgacatattt gattgttgtc agtagagtca atgtccaaaa aatcaatgag actgatccga
    10141 taacaaaatc aggattgatt cctgacaatc cgccattatc ttcaacaatc gatttcataa
    10201 cgtaaagcgg acttgtcccg atgtctccgt agacgactcc catcgctaca agcaggccag
    10261 ccattgatat ctttttggta ttgtgcgttg ttgccacgtt tttccctcat ttcttgttat
    10321 atggatatcc tttaggtaag cttgaatgat agatgatatt cttatccatg acacccgctt
    10381 tcctaaattg cttctaatta tccaagtaac acactgaagt gtcaacttat ttgaataaaa
    10441 ttattctttc tttggacgtt ctctgatacg tcttgccaca aatgaataaa gagcaaggca
    10501 aaaaattgca cccaataatg ctggtatcaa ataaaaaccg ccgagttccg gtcccagtgg
    10561 tcctagtaaa aattcgccga gccaactacc taaaaaaccg ataatgatat ttccagatac
    10621 gcctcccggg acttcctttc ctaaaattgc gccagctatc aaacctaata taccgccgat
    10681 aaataacgac cataaaaaat tcagcatctc ctttccctcc tttatccata tttctcactt
    10741 ttattttacg ctttattcag aaggactcca aaaatatgcg ccaagaaact ccagaaaaaa
    10801 agaagttgta acaaaagtgt tcagcttatt tccgtagaag ctgcttctgg aacaccgtga
    10861 atcagaggtt gtgaaatcag cggtttgacc tgagtattag agaaacaaac ggaaaaatag
    10921 ttcctcatat tttgccgttt tttgagctaa tatcgaggga cagctgattg aacatcgttt
    10981 attggagagg ttgtaaaaaa gctgttctgc atcaagtaat aagaacagcc aagtaaaaac
    11041 agctcctaat gtttttaact tggctggact tattaccgca gatgcaagct tttgaacacc
    11101 gtttattcga aaaaaaagga ctgtgacacg gcttttgtca cagtcccctt ttttcgaata
    11161 attattgtcc ccctgtcacc ttcacgatca caagtacatt attcctatga attaaattat
    11221 ttttcttcat ttttcgttga accatcttcg ttcaacattg cttttcttga cagatttacg
    11281 cgtccttgtt tatcgatttc agttactttc acaaggatct catcacctag tttaacaaca
    11341 tcttcgactt tattgacacg atcgtttgct aattgggaaa tatggacaag tccatctttt
    11401 cctttgatca agttgacgaa tgcaccgaat ttttcgatac ggacaacttt ccctagataa
    11461 acttcaccaa ctttgacttc tttggtcaaa tcttcgatga tcttgatagc ttttttgatc
    11521 atttctgcat cagctgaagc aatacttact tttccatctt gatcgatatc gattttcaca
    11581 ccagtttcat cgatgattcc gttgatcgta tcgccgcctt taccgataac atctttgatt
    11641 ttagcaggtt cgatctggat catttcgatt tttggtgcat attggcttaa ttcttcacgt
    11701 ggagcagcaa tcgttgaagt caattcttct aagatttcca tacgtgcttg ttttgcttga
    11761 gttaatgctt cagttaggat ttgttctgtg attccttgaa tcttgatatc catctgtaaa
    11821 gcagtgatac catcttttgt accggcaact ttaaagtcca tatcgccaag atgatcttcc
    11881 agcccttgga tatcagttag gatcgtatag ttttcaccat cagaaacaag tcccatcgca
    11941 ataccggcga ctggcgcttt gattggtaca cctgcatcca tcaatgctaa tgtgccagca
    12001 cagatacttg cttgggaaga agaaccattt gattccaata cttctgctac taaacggatc
    12061 gtataaggaa attcttcttc tgaagggatt acttgtgcta acgcacgttc acctaatgcg
    12121 ccatgtccga tttcccgacg ccctggagag cctgcacgtc ctgtagaacc aacagaaaat
    12181 tgagggaaat tgtagtggtg gatgaatcgt ttgctgactt ccacccctaa accatcaatg
    12241 atttggtgct ctcctagagg tgcgagtgta caagctgata aagcttgtgt ttgtcctcgt
    12301 gtgaacaaac ctgatccatg tacacgtggc aataagctga cctctgatga caatggacgg
    12361 atttcatcca atttacgccc gtcaggacgg atcttatcga tggtgatcaa ttcacgtacg
    12421 acatcttttt caagatcttc tgtgatttgt ttaacttctt tcaacaagtg atcttcatct
    12481 tcatgacctg caaatttctc agcataagct tcgcggatat cgatcttcac ctgctcgata
    12541 ttatcttcgc gagcaagttt ttcttcagtc attactgctg ctttcattgt agcgtaagaa
    12601 gcatcaaaga tttctttttt caatacttca tcaacttgca gtaaagtcac ttccattttt
    12661 tctttcccta ctgcttgaac gatttcttct tggaaagcaa ctaactcttt gatagcgtca
    12721 aaaccaaaca gcaatgcacc aagcatgtct tcttctgaaa cttctttcgc gccgctttca
    12781 accatattga tggcttgttt cgttccggca acagtcaatt cgatatctgt ttgttctgct
    12841 tgttctactg ttggattcaa gacgtactcg ccatttacac gaccaacttc tacgcctgcg
    12901 attgggccgt caaatgggat atctgagatt gatagtgcta aagaagaacc tagcattgct
    12961 gccatagctg gcgagcagtc tgtttctaca ctcatcacga tgttcgttac ttggacttca
    13021 ttacggaagc cttctgcaaa catcgggcga attggacggt cgatcaaacg ggcagtcaat
    13081 gttgcttccg tacttggacg gccttctcgt ttgatgaaac ctccagggat tttccctgct
    13141 gcatacattt tttcttcata attgatcgtt aatgggaaaa agtctgtatc ttttgcttct
    13201 ttagaggcca cagctgcact taagactact gtatctccat aacggactaa aacggctccg
    13261 tttgcttgtt ttgctagctg accgacttcg atttgtaagg gacggccgcc ccatgtcgtt
    13321 ttgaatactt gtttttctga cataacttct ccttttcctt ctcgtgaaag caagacgcta
    13381 ctaaacaaat gaaaactgcc gaaagccgtc ttttgagcag ctttcatttg gttagtagga
    13441 gtccatctag cttgatcacg gaattttact taattgatat acgagtaaaa aaagtgagat
    13501 tcatgtgaat cccactcttt ttgactaaaa ttaacgacgc aagcctaaac gtttgatcaa
    13561 ttcgcggtaa cgttgaacgt cagttttacg taagtaagct aacaagttac ggcggtgacc
    13621 aacttttttc attaggccgc ggtatgaatg atggtctttt ttgtgcacgc gtgcatgttc
    13681 attcaagtgg ttgatttctt cagttaatac agcgatttgt acttctggag aaccagtatc
    13741 cccttcatgg cgagcgtatt cgttgatgat ttcattcttg cgttcttttg aaattgccat
    13801 ttctttcacc tctttcgatt attcccctgc gactgagtaa aacgttggtg attcgcttaa
    13861 ccaagtggca ggtcatgtgc ctagagcaca cagctatact ttacctgaaa ccgggttgaa
    13921 aatcaagtag ttaatcatta agaaaaggta gttttcaaac aatcttacta ctacttttcc
    13981 atttttgtgg taaactataa tgcgaaaata gacttgaaac acagtctttt aagaaagggg
    14041 tacatattcc atgattacaa tggatgatat cattcgtgaa ggaaatccga ccttacgcga
    14101 agttgcaaaa gaagtttctc ttccattaag cgaagaagat atctctttag gaaaagagat
    14161 gcttgagttc ttaaaaaaca gccaagatcc aatcaaagct gaagaactac acttacgagg
    14221 cggtgttggc ttagctgctc ctcaattaga tatttctaaa agaatcattg ctgttcacgt
    14281 accaagtcct gacccagagg cagatggacc ttctctaagt actgttatgt ataatcctaa
    14341 aattttgagc cattctgttc aagatgcttg tttaggggaa ggtgaaggct gtttatctgt
    14401 tgatcgagaa gtaccaggat atgttgttcg ccatgccaaa atcactgtgt cttattatga
    14461 tatgaatggt gaaaaacaca agattcgttt gaagaattat gaatcgatcg tcgttcaaca
    14521 tgaaatcgac catatcaacg gtgtgatgtt ctatgaccac atcaacgatc aaaatccatt
    14581 tgcactaaaa gaaggcgtat tggtgatcga gtagagaatt ggcaggttcc ttcgatacta
    14641 tgctacaaga aagctttata aaaaagggga gtgctcaaaa gctgcacttc ccttttttat
    14701 gtttctccac taatcgaaat ttagatagac atctagaaat cgcaattcca aaaaattctg
    14761 tgtattggat acgcatcttt tcttattttt tcttatttct tccatatggg tgtttatctc
    14821 agagatgata gaatagtttc taggataaaa agtagcagct acgatgttat ccttcgtaga
    14881 ggattcctgc ttcagctagt ttttgtttga tgtttttcag catatgcttt tctgggtagc
    14941 ggatcgtatg cagatggatg ccgcctgtta attcggataa caaagtagca ttactttttt
    15001 gataacgtct cataaaggaa tcgatatctt gtggcgtttt gatgtgcaaa gttcctgcta
    15061 actcaccgta aaggggatgt tccacaatta catcaatgat ttcaccacca ttttcaacga
    15121 ttagtctcag ttcttcttct gtttgatctt ttccatgctg gacagcgatt ttactgatgt
    15181 gcccagtatt ggtttcttct gtttcgagga tataacctct tgctgttgcc acgatacttt
    15241 tacctgcagc tcgtagtaaa gcaatatcgc ctacaatgat ctgacggctg actccaaaag
    15301 ttttcgcaaa cttgctagca ctcactggtt tttctgtatt tgccaaggtt tgcatgattt
    15361 gttttcgccg tgtctctcct tcgatcacac ttattcctcc ttcattttgt tttggtattc
    15421 gcgcgagtgt ttcatcaaat caacatacgt gtctgttatt gtttccttat atcccttatt
    15481 tttcagcaga ctaacaactt tatccaatac ttgctgctct ctttttgcat cataaactgc
    15541 ttggttttct tcttttttca cttgtcctat tttagttacc gtatccattc ttttttctaa
    15601 taaagcaacc aactctcgat cgatcatatc gatcttatga cgtaatgttt caagagactc
    15661 ttctgttagt tcttcctcta ctgtcttgat gatacgagca ggatttcctg caatcactac
    15721 attgtcggga aaagatttgg tcacaaccga acctgcgccg actactacgt tgtcccctag
    15781 agtgacaccc ggcgtgacga taactcctcc gcctaaccaa acattattac cgattttaat
    15841 gggttttgcg tactccagac cgctgttacg tttcactgga tgtagtggat gcgtcgccgt
    15901 gtaaagctgg acattcggag cgaacatgca gttatcaccg atttcaatcg tgctgacatc
    15961 taaaaatgta caattgaaat ttgcataaaa attctcccca acaaatatat tatatccgta
    16021 atcaaaattg ataactggct ccatatagat ttttttccct gtacggccaa aagtctcttt
    16081 caacaattgg gatcgcagtt ccgctgattc tgcttggttg atgatttggc tttgacttct
    16141 tgcaaacata cggtcggcaa ccagctcagg atcattggca aaataaagct cgcctgcaat
    16201 cattttttct ttttcggttt tgaattcagt cataatctgc tcctaattct tgtatttttt
    16261 tgaaaacaac cgctccgtct tcttgaataa acgtataagg ctccgctaaa taaacaggcg
    16321 tttgattgat tactaagatt ttggctgacg gctggcgaaa ctgtaccaga tcacaaaacg
    16381 gatgaacttg aaaactcgtt ccaacaatga cgatcagctc tgcttggctg acggcataag
    16441 ccgcctgctc catgactcca tcttgaaacc cttcaccata taacacgatg tctgggcgta
    16501 tttgtcctcc gcatgcttga tgtttgtcgc tgtgaagata ttctttccag tctaccgttt
    16561 gttgacattt tcgacaatga cattgataca gactcccatg aaaattgacg agttgcgggc
    16621 ttttagcctt tgcgtgcaaa ccgtcgatgt tttgggaaac aacccaaaca tttttttctt
    16681 tcgctagatg tgctatttcc aaatgaatga tattgggttt tgcttcagga tgataaatct
    16741 ttttgacaaa ttgatagaat ttatccggtt cattcaccat tgcttgatga ctcagcaagt
    16801 attccggctg ctccatcccg tggtagattc ctgacaaaga tcgatagtct ggcactccag
    16861 aaggtgtgga tacccctgca ccggtcaaaa atgtaatttg ctgataagct aatatctctt
    16921 ttgctgcttc gtctactgtt aattgtttca tgttttgtca cctcactttc cattatagcc
    16981 catactcgaa caaatttatt gttgaaaagt cagaaaaatg ggaataaaaa agtgttatgg
    17041 gcatttgagc aatttatcac aatgttcact gacaaaagaa tgaaaacgtg ttacaatact
    17101 gctgtataaa agtgaggtga atcgatatgt taccattctt tatttattgg ggcgttattc
    17161 tggcttgtat cgcctggtta gcgttaagcg tttatttctc tgttttttat cttgtacgca
    17221 aagaaaatgg aaatctttgg gcttttgctt tattcaacgt gattgccgca attgttttag
    17281 ccattacatt agccgtatat cgaacatggg gctggggaat cacccaatac tcaagtttga
    17341 tttatctaat attagccatt tatggcgtcg ttgttctttt gcaagcgatc ctaggacgtg
    17401 agccaaagaa agcggcagct taaatgatcg agatggaact ggacacagga aattttctgt
    17461 tctagttctt tttttattgt attacataca tttttgtgta taattatact atcgtttgca
    17521 atggatagat gattcacatt ttgattgttt tttcggtaaa agtaaagatt tctatattta
    17581 tcagtatttt ttcaaaaacc agcagttata gattgacaaa gcctctcgag tatcgtaaac
    17641 tttttttgta taaataactg aatacgagat gcctcaatca atgaggtaga ggtcgcgaga
    17701 atcattaacc ctgtggagtg aagcaaacat ggagaagcag gaatagggag gatcgccgaa
    17761 atgtgcactt ttttgcttgg agtgtatgtt gggacgcaag ttaagagctt gcggactgtc
    17821 ttagcagtga tgctaagttg cgctatgttt tgtgagaggt ttatcacctt atggaatttt
    17881 agcggccttt ttatatctat aaatcggtcg ctttttattt tttcagataa agctttaccg
    17941 catttcatct agaccgctcg tgtatcatat aatcagaaag ggagaaaaaa atgaaaaaaa
    18001 atatggtttc agttgccgta ctatttgctt ttgtcttctc actattcagt gtgggatctc
    18061 tggcaaatgc agatgaaaaa acaccgagcg atgagtttcg cgtaggtatg gaggctgggt
    18121 atgcaccctt caactggtct caacaaacag atgagaataa tgccttgcca attcaaggcc
    18181 aaaatagtta cgccggcggt tatgatgttc aaatcgctaa aaaagtagca gatggtttag
    18241 ggaaaaaatt agtcatcgtc caaacgaaat gggatggtct ggctcctgcc cttcaatcag
    18301 gcaagatcga cgcgatcatc gccggaatga gccctactgc tgaacgacga aaagaaattg
    18361 attttaccga tccttattac gaatcacaat tagtagtagt agttcaaaaa gatagcaaat
    18421 atgcaaaagc aaaaagttta gcagatcttt ctggagcaaa gatcactgct cagttaaata
    18481 cattccacta tactgtgatc gatcagatac cagatgtgca gaaacaacaa gcaatggata
    18541 atttctcagc aatgagaaca gctttagctt ctggaatgat cgatggttat gtcagcgaac
    18601 gtccggaagg tgtaacggca actagtgtga acaaagactt ggaaatgctt gaattcagcg
    18661 gtgcagatgg cttccagaca aacccggaag atgttcagat ttctgttggt atgcgtaaag
    18721 gcgacccgga tatccaaaaa gtaaaccaga ttctttctgg catctctaac gatgaacgaa
    18781 ccaaaattat ggaccaagca gtcaaagatc agcctgctgc aactgatagt gaagaaaaac
    18841 ctggcatcat ttctgatttc actacgattt ggaaacaata cggcaatatg ttcttacgag
    18901 gtgctggact taccttgttc atcgcattga tcgggacagt tgtcggtact acacttggat
    18961 tactgattgg tgtcttccgt tcggtacctg aatcaggaaa tgcactgact cgcttcttcc
    19021 aaaaaatcgg aaatgcactt ctttctatct atgtcgaagt attccgcgga acaccgatga
    19081 tggtacaagc aatggtcatc ttctacggtc tggcactcgc ttttggcatt tcattgaacc
    19141 gaacgattgc cgctttgttt attgtctctc tcaatactgg agcttacatg acagagatcg
    19201 ttcgtggggg gatttttgca gtagatgaag ggcagttcga agctgcgcaa gccattggta
    19261 tgacccatgg ccaaacgatg agaaaagttg ttattccaca agttttacgg aatatcttgc
    19321 cagcaacagg aaatgagttt gttatcaata tcaaagatac agcagtttta agtgtaatcg
    19381 gcgtagctga tttgttcttc caaggaaact ctgcttccgg tgcgaatttc caattcttcc
    19441 aaacatttac gatcgtcggg atcatctact tagtcatgac ctttacgatc accagaatcc
    19501 tgcgggcggt agaaaagaaa atcgatggtc cttcagctta taccaagctt gaagaaatcg
    19561 acaattcaaa tttgcaggaa taaggaggaa gaatcatgaa tgaaaaaatt attgaaatca
    19621 aacatctaag aaaaagcttc ggtgaaaatg aagtattgaa agatatcagc gtaacagtca
    19681 ataaaggtga agttgtgacg atcatcggtt cttccggttc tggtaaatct actttcttgc
    19741 gctgtatcaa tttactggaa aaaccaacag gcggcgagat catttataat ggagaaaatg
    19801 tattagctcc taaatataat ttacctaaat atcgaacgaa tctaggtatg gttttccaat
    19861 ccttcaacct gttcaacaat atgaatgtct tggaaaactg tatgtctggt caaacaactg
    19921 tcctgaaacg agataaagaa actgccaaaa aagtagcaat cgaaaatctg gaaaaagttg
    19981 gtatggatcg ttatatcgaa gccaaaccag cacaattatc tggtggacag aaacagcgtg
    20041 tcgcaattgc tcgggcatta tcaatgaatc cagatgtatt gctattcgac gaacctactt
    20101 cggcgttaga ccctgaaatg gttggcgaag tgcttaatac gatcaaagac ttagcacata
    20161 ctggcttgac aatgcttatc gtgacgcatg agatggaatt tgcaaaagat gtatctgacc
    20221 gtgtcatttt tatggataaa ggtgtgattg cagaagaagg tacaccagaa gatatctttg
    20281 tccatccgaa agaagaacgt acaaaagaat tcctttcacg tattttaaat gcttaggatc
    20341 aatagctgat tgggacaaag agctgcggtg tttaccgcag ttctttttta tgaattactc
    20401 ataaaaggta aatttctgaa aattttggaa tcttagttgt gtttttcaga aaactctctt
    20461 aatatggaag taattaaact tttttagtta ggatgattta aaaatgaatg aagagcaaga
    20521 actgcagcgt ggactgaaaa atagacatgt ccaactgatt tctattggcg gagcgattgg
    20581 aacggggttg tttttaggtt ccggaaagtc tatccagcta gctggccctt cgatattgct
    20641 cgcctatctt attactggat gcatctgttt tttcattatg cgtgcgttag gcgaacttct
    20701 gttatcaaac accaataacc attcttttct ggattttgtt gcagaatatc ttggcaaaaa
    20761 agcggctttc atcactggct ggacgtattg gttctgctgg gtatcgattg caatggccga
    20821 tttaacagcc atcggaatgt atgtacgcta ctgggcgcct ggtgttcctc agtggcttcc
    20881 tgaactgatt gcattaggtc ttcttctttg cttgaatatg gtggctgtct cattgtttgg
    20941 tgagttagag ttttggtttg cattgatcaa agttgtagcg atcattgcct ttatcctcgt
    21001 aggagcttat atgatcctca cccactatac gacagatatt ggcacagcca gtatcacgaa
    21061 tctatggtct catggcggat tctttccaac tggtgcaaaa ggatttatct tagctttcca
    21121 aatggtaacc tttgcttttg ctgggataga attagttgga ttagtcgctg gagaaacaga
    21181 aaatcctgaa aaagtgttgc cggaagctat caataatatt cctatcagga tcatcttatt
    21241 ttacttagga tctctttttg tcattatggc aatctatcca tggaacagct tgaatccaga
    21301 taacagtcca tttgtagaag tattttcgga aattgggatc acgattgctg cttccttgat
    21361 caatttagtc gtattaagtg cagcagcttc tgcctgcaac agtgcaatat atagtacagg
    21421 acgtatgctt cgttctttag cgcaagaagg cagtgcccct aaaaaattca aaaagttaac
    21481 aacacatcat gtgccaggga atgctttgac attttctact atcgtcattt tcatttctgt
    21541 tatcttgaat tacgtgatgc ctagtgaagt attcacacta gtaagcagta ttgccactac
    21601 ttgtttcttg tttatctgga gtatgcttgt ctacacgcac atgaaatatc gtaaaagtat
    21661 cctcggcaaa aaagctcatt catttaaaat gccgttgtac ccatttagta attatctggt
    21721 atttctttac atggcctttg tgtgtgtcgt cttgttccta ggaaaagata cgagaatcgc
    21781 tttattacta acacctgtgt ggttcttgct tctattacta atctatcata tgaaatatga
    21841 acgtaagaag taagaaagta gttgtgacag aagtggtcag cttcgagaaa taagaaggaa
    21901 tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttcttg gagataaaag
    21961 cttctgtccc agcctctttt ctatttcccc ggaaacaacc gctgataata ggcatctgct
    22021 tttttatagg aaaaaatcca aataccagat acaaaaaaac gtccgaataa cccgatcaac
    22081 ggtacaaaaa agacaccggc aatcaagcat aagttaatca tcatggagat aagacttcga
    22141 cggtcctttg acaaaactct cacgacatgg gaatctggtt tatttgcttt catgatttgc
    22201 ctcatcaaga taccaaaaga aaaactccac atcgtgtata ctaagacata agccatctca
    22261 ggccaaaaag cttcagggtt acgcccgacc caactagtaa cagaaggaca taaagtcagc
    22321 caaaagagca aaaagatatt cgaccagacc actcggtaat taattcgttc tgccatctga
    22381 aatatacgat gatggttcac ccagatcacc gcaacaaata taaagctgat ggtataagct
    22441 aataatgtat ttcggatgac ccataaagaa tgaaagctag atgtattagg gatcttgatc
    22501 tctaatacaa taactctcaa aacaatggct accactccat cagtaaatgc ttctaatctc
    22561 gactttgtca cgatgattcc tcctcagatg attcagcgct tttattttat cacttacgtt
    22621 attaaataac catcgtttta tatagggaat tttctgaaaa ttgttgcata tttttcgaaa
    22681 ttttgttact tttaaaaaaa ccgtaaagga gttatgtatg acaaaaaaat tatcatttaa
    22741 agactatctt tttataggct ccatgctttt cggtctattt tttggtgctg gaaatcttat
    22801 ctttcccgtt catttgggtc aagaagcagg cgctgctacc ttttgggcga atctaggttt
    22861 cttggttacc ggtatcggtc ttcccttctt aggggtgatt gccattggtg tatcgaaaag
    22921 ctcaggtgta tatgaattag caaaacggat aaatcaaaca tatgcgatgg tttttaccat
    22981 cttgctttac ttagttatcg gaccgttttt tgctttacct cgccttgcaa ccacatcttt
    23041 cgagattggt ttagcaccat ttatcgatcc tagcagacag acgctatatc tagcaatttt
    23101 cagtattgta ttttttgtgc tggcttggtg gttttcaaga aaaccgacaa agatattaga
    23161 ttatgttgga aagttcctga atcctgcatt tttggtttta ttaggtgctt tgcttctcct
    23221 tgcttttttg aatccactag gttctgtaaa tcaagcgcct attcagccaa attatcaaga
    23281 gcatgctttt ttcacagggt ttactcaagg atataacaca ttagatgcgc ttgctgcttt
    23341 agcatttggg atcatcattg tcacaacaat ccaaaacatg ggtgtaacaa aacctgctga
    23401 aatcgctaaa gacacgatca aatctggtgc gatcagcatt atattgatgg gaatcattta
    23461 tactttgctc gcttatttag gtgctatgag cttaggtagc tttgctctaa gtgaaaatgg
    23521 cgggatcacg ctggctcaga ttgctgatca ttatttaggt acttatggca gtatcttatt
    23581 agcatttatc gttatattag cttgcttgaa gaccggtatt ggtttgatta cagcttttgc
    23641 agaaacattc acagatttgt ttccgcaaag aaattacgca ttctttgttg cacttgctag
    23701 tatccttcct tgtttagcgg caaacgtagg cttgacgaat atcattcaat tttcgttgcc
    23761 tgttttgatg ttcatctatc cattagcaat gacactgatt ttactagtgt tagttggtcc
    23821 tttgtttaag cagcgtacgg ctgtttatcg aatgactact tattttacat tgattgcttc
    23881 gatttttgat gggttgaacg cttgcccaga aactatcaaa caaacaccta ttgttcaaaa
    23941 tcttttatat gttgcggaaa gctacttgcc gtttttcaaa ttggggatgg ggtggatcgt
    24001 accagcagtt atcggttttg ttatcggact gatttggagt ttcgctaaga aagaagaagt
    24061 agcagattga taaaagaggt tgtgaaagag acgttcagcc ttgagcaata aaaagtaatt
    24121 ttgaaaaatg gcttctcaca tttttgcata aatttgactt aataccgaga aactgacttt
    24181 tgaacaccgt ttattgagag gtcgtgaaaa agccgcttag tttcaagtat taagaaatat
    24241 tttgtgaaaa tggcttctca catttttgca taaatttgac ttaataccga gaaactggct
    24301 tttgaacacc gtttattgag cgtctataaa ctgacctgag tattagagaa acaaaacggc
    24361 aaagtagctc catatacttt gccgttttgt tttgaactaa taccgaagag aagctgatca
    24421 aacaacattt cttttataat a
//
LOCUS       DAZKNO010000023        24376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000023 DAZKNO010000000
VERSION     DAZKNO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.023"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..961)
                     /locus_tag="ACG8MY_000693"
     CDS             complement(47..961)
                     /locus_tag="ACG8MY_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0429916.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(976..1977)
                     /locus_tag="ACG8MY_000694"
     CDS             complement(976..1977)
                     /locus_tag="ACG8MY_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0429917.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            complement(1974..4157)
                     /locus_tag="ACG8MY_000695"
     CDS             complement(1974..4157)
                     /locus_tag="ACG8MY_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0429918.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            complement(4160..6607)
                     /locus_tag="ACG8MY_000696"
     CDS             complement(4160..6607)
                     /locus_tag="ACG8MY_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0429919.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(6591..6983)
                     /locus_tag="ACG8MY_000697"
     CDS             complement(6591..6983)
                     /locus_tag="ACG8MY_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0429920.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            complement(7070..7567)
                     /locus_tag="ACG8MY_000698"
     CDS             complement(7070..7567)
                     /locus_tag="ACG8MY_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0429921.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(7684..7908)
                     /locus_tag="ACG8MY_000699"
     CDS             complement(7684..7908)
                     /locus_tag="ACG8MY_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429922.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(8012..9922)
                     /gene="ltrA"
                     /locus_tag="ACG8MY_000700"
     CDS             complement(8012..9922)
                     /gene="ltrA"
                     /locus_tag="ACG8MY_000700"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0429923.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(10706..11911)
                     /gene="mobT"
                     /locus_tag="ACG8MY_000701"
     CDS             complement(10706..11911)
                     /gene="mobT"
                     /locus_tag="ACG8MY_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0429924.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(11934..12087)
                     /locus_tag="ACG8MY_000702"
                     /pseudo
     CDS             complement(11934..12087)
                     /locus_tag="ACG8MY_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(12090..13475)
                     /locus_tag="ACG8MY_000703"
     CDS             complement(12090..13475)
                     /locus_tag="ACG8MY_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0429925.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(13504..13890)
                     /locus_tag="ACG8MY_000704"
     CDS             complement(13504..13890)
                     /locus_tag="ACG8MY_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0429926.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(13906..14220)
                     /locus_tag="ACG8MY_000705"
     CDS             complement(13906..14220)
                     /locus_tag="ACG8MY_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0429927.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(14573..15052)
                     /gene="rlmH"
                     /locus_tag="ACG8MY_000706"
     CDS             complement(14573..15052)
                     /gene="rlmH"
                     /locus_tag="ACG8MY_000706"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0429928.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15480..16769
                     /locus_tag="ACG8MY_000707"
     CDS             15480..16769
                     /locus_tag="ACG8MY_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0429929.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(16808..17413)
                     /gene="ytpR"
                     /locus_tag="ACG8MY_000708"
     CDS             complement(16808..17413)
                     /gene="ytpR"
                     /locus_tag="ACG8MY_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0429930.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(17609..18073)
                     /locus_tag="ACG8MY_000709"
     CDS             complement(17609..18073)
                     /locus_tag="ACG8MY_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0429931.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(18095..18415)
                     /locus_tag="ACG8MY_000710"
     CDS             complement(18095..18415)
                     /locus_tag="ACG8MY_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0429932.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(18558..19637)
                     /gene="pepA"
                     /locus_tag="ACG8MY_000711"
     CDS             complement(18558..19637)
                     /gene="pepA"
                     /locus_tag="ACG8MY_000711"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0429933.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            19837..20187
                     /locus_tag="ACG8MY_000712"
     CDS             19837..20187
                     /locus_tag="ACG8MY_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0429934.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            20372..21325
                     /locus_tag="ACG8MY_000713"
     CDS             20372..21325
                     /locus_tag="ACG8MY_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0429935.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            21338..23125
                     /locus_tag="ACG8MY_000714"
     CDS             21338..23125
                     /locus_tag="ACG8MY_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFQ0429936.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            23112..24314
                     /locus_tag="ACG8MY_000715"
     CDS             23112..24314
                     /locus_tag="ACG8MY_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429937.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         6853 a         4936 c         4472 g         8115 t
ORIGIN      
        1 gagagtacgt gattacaaaa tagctgtaat aatgtaccaa tatttgttat tctataatct
       61 tccaattact cccgttcttt tcaagtacca aatcaaattg agatacctgc gttgctttgg
      121 tctgctggtc gatatactcc actgtcagcg ataccgtgac ttgattatcc ttacgattgt
      181 aaataggatt taccagttct tgaaagatgt actcttttcc gattggtttt aatatcccgt
      241 cattcacata gtaggaaagt tcactggctg tcgctgtagg atagagcttg aagaacgtcg
      301 ttaaaaactc attgatttca ttggttgtaa tggaatcaac cgtaccctca ctttcaatag
      361 cttttggttt atagtctgat ttctttggta tgctagtaat ggtcggattc ttaatgagga
      421 ccatatttcc agtactgtct acatagacac tcactatata agcagagtgg acggtctttg
      481 tattttcccc ctctgtaatg agttggtcta cactgtaggt tacatcaaat tgattgtcgc
      541 catctgcttc taccgtccat atctgaaatc cttttacaga agacgataca ggaatatctt
      601 tacgaactgt atcgacattc agagcttgaa gttcctctgt cagatatgct ttcaggtttt
      661 ctatgcgatt atcaatggac ttgtcgttta gttcccatga atagtagact ttcgcaaagt
      721 tctctacaaa attttccaca tgatgagtat cgacatattc cttttctatg atggtggttt
      781 catgaatggt atgggtatca atggctgtaa agtgcttgaa tatcgcaaag ctgaaactaa
      841 gtcctaaaag tacccatagg acaatcacaa cctttttacg aggattgacc ttatagacac
      901 gaggtttctt ttcctttggt gtctgttttt ctgtattctg attttttccg aatttcatca
      961 ttgaatcgtc ctttcttatt gtttgattcg tcctgctccc actaaatgct gttgccagta
     1021 agagcttgtt aagtcggcat aaccgatagg gtcgcctgca tgaaacatac ggttatcgcc
     1081 taaataaatc ccgatatgag taatataaga gccagcgtta taggtagaat gaaagaaaac
     1141 caaatcgccg gcttttgctt ccaataatgg gatatgccgg gttacatcat attgctgttg
     1201 tgctgttcgt ggtaagttga ttccagcttt tccatacgtc cattgtgtca gtccgctaca
     1261 atcaaaagag gtagtcgggg aagctccgcc atagacatac ttccagcctt caaatttcag
     1321 agcttcgtcc atgatggctt gtactgtatc atcatcaaac tctgttgtga caagatactg
     1381 cgttaccagt tgcacataaa acatattccc gtaattgtat cgccagcccc cattgatagg
     1441 tatggctatg ggattggggt aagacacttt ttcgtcaccc gaatactctt ttgagaaact
     1501 ttgagccagt tcaaaggtat atttattccc acgattagcc acatatccta agaaaccacc
     1561 accataattg taggactgga taaccgattc taaatctaca ccaagccttt cgctactggc
     1621 taataactca ctgaaatact tcacaccttg cttaatggat tcttctgtac tcaatgaatt
     1681 aggtggaaga ccgagggatt ccgaggactg cataacatct tctgctgtac cacccgattc
     1741 cacctgtata atcgcaagaa gtatattgac gtattcttca attccatatt ctttggcaac
     1801 tttttctacc ataggcttat gagccagcac ttctgcggaa acatccacac cgccatagtg
     1861 aatgttggaa aaaccgccat cttgttcatc tgaaaataaa atggcgacaa acagaagcag
     1921 tgagaagacc atcaagaata atcccgaacc accaatcact aaagttttca gcttcatggt
     1981 ttcttactgc ctttcttaat ggtggcggtt ttgattggtg gtctactttt tgtattttgt
     2041 agtggtactc tttgaacagt agactgacgt tcttttgtga ttgaacgttg tgaagctcta
     2101 tctgttggtg cagttgaagt tactggcttt tgaaccgttc tttcttgaac tgtattactt
     2161 tgacgttccg tttttaaact tgaagaatcg gacttaactg ttggacgctc ttgtttggct
     2221 tgttgagatt ccctatagga agcctgtaca gttgtttgcc ttgaagtctg tccatcatga
     2281 gattgttctt gcttagtggc tggtctttca tgaacggaag aagctggctg ttttttctgt
     2341 ttgacctgtt ccatttcaga gcgacgctcc gcaatggttt tccgtctttg ttcctgctgt
     2401 ttcttgcgtc cactggcttt atccgctttg gtttgagaaa tacttctggt taaatcatgg
     2461 acattatccg tcactttaga ttttccttga tatactgcat atcttgcatt ggtcggcaaa
     2521 tccttaacct gttctttcaa attaccagca gtatctacca ttctgtcttt ggtatcagct
     2581 actgtaccga tggtttgacc gatacgcttt cctagtgttg atttttcctg cccgtttggt
     2641 cgagagtgat ctgcttgtgt actcgcagaa cttcccgaac ccgactgtcc ttttttaccc
     2701 gtagcactgg caatggcaga cccagcccct aaagcagtca cggagcgtcc aagtttccgc
     2761 tgtagacggt gcatgtgagc gtgcataagc atacgaggtt ttctcatcat acgacttccc
     2821 acactttgag aatcgttact ctgtagagaa aacatactca ttaaatcgcc cagcttaaag
     2881 tagattcctg caaaggtcac aatctgtaga aaagcaatca aaaagaacgg ataaccagcc
     2941 gataaggtat agagcatggt tgaaatacta aatgctgtcg taataatcaa tgtgattcca
     3001 gctcgtgtca aaatggtatt aaagagcttt gttatggctc gttttgacat accatcaaat
     3061 gatggaatca tgcttaaaat aaagctcaca ggcagaaaca tagcatagat gataaaaagt
     3121 acctgcgaga aaatcatgat tcctgttaat aggaatacaa atatggaaat cccaatattg
     3181 aagacaaata ggaagaagac tgtacctaaa cggttaatcg tctttgtaat cgtcatattg
     3241 gtattgcttc tatcttcaat ttcttccgca acaatttttt ctctgtcttc cccattgttg
     3301 gaatctggac tggttgagag caggctttcc acacggtcaa taccaatact ttcaatgtct
     3361 gaactgttat attgaagcag tagccacggt tgctgaacct gtatggaaaa taggctgtca
     3421 cgtatcaagt ccacactgtc cttgccttga ctatctgaat ggggcatgac aatttttgta
     3481 cccagtgaca aactagcgtt gctgatgtct gatgaaaagt cattgatttt tttaatgtag
     3541 tcgggagcgt aggcaataaa ggaagccgat aggataaaaa ccagcacaaa attcatgagg
     3601 gcatgaattg cctttgtggt ttctctcttt atcagtcctg tataggcaac ataaacccca
     3661 agaaccaaaa tcaagagtaa aaggaatcca acatagaaac cctctgttga tagtccacta
     3721 ggcgtaaccc ctgcaagggt ctgcatattc ttgccaatgg aatctgctgt agcggaaatg
     3781 aagtctaagg aataggcttc ctgtactaag taacccgtcg cattggaaac ataaagactg
     3841 attgtccaaa taaaattggt aatggcatat agtccataca tgacttgttt tccaattccg
     3901 tccgaccagt tccacggtag ccagccccag ctattatcca cataaaaatc cagttgatag
     3961 ttttcaagtg ggtatcggct gtattcattt gccacattga ccgtatcatc taccaagccc
     4021 gcagcttgaa ccaccgttcc cagcatggct aaaagaaaaa tggcaatcac aagtgtgaaa
     4081 gccactgtca ttgccacttt acctagacgt ttcagcgtcc agtttgattt tattctgttt
     4141 aatattgatg gtttcacatt tacacctctt ttcgcacagg tggtctggta tcaaaggcat
     4201 ggagcagttc ttcaaataca gggtggaact gtatcacacc gacacgacca tataaatcac
     4261 tgataaggca ttgcccgttt tccaaatcac gcaatcgctt ctgattgttt tcgtcctctg
     4321 ggtctacacc aaaaaaggca agggtttttt taatctcgtt aaggtcagtg gaacgaaagg
     4381 caaattttaa gccgaggtta tttttcagtt tttcatctaa gaggtcatct gtattttggg
     4441 tcacgaaata taccccagcg ttcatagcac gaccagcacg aaccagcttc atagatagtg
     4501 ttttcccttg tgccacctgt aagaagctcc atgcttcgtc taagtctaca atcttgaaaa
     4561 tacttcggtc tgtatggata aagtccaacg caaaggtact aatgacaatc agcattgcta
     4621 ccgatagtaa ctccattgtg gtatattcct caaaggaagt ttccttgtca ggaagtacca
     4681 agtcggcaac ctgtataatg ttcagttgtt tttcaaggct gatagactgc tctacataac
     4741 catcactaaa aagcagatgg gcaaagtcat agtctgtaaa actttcgata tggtcggcta
     4801 tgctggtact tagtggcgta ttctcaaccc gtaattcttc aatcactttc atcaaccctc
     4861 gcacttcact attggttact gcacgaatgg cttttctaag gattgggaag cgttccccat
     4921 cacgagagga aatccccgta aggaatgtca aaatatcaat agccagtgat tcagaatctt
     4981 tggtattttt cataattaca taagggtcaa gtaagccttt gtttttctca tcagaagtca
     5041 gattgacgat attgatttca tgggaaatct ctggcaaggt ttctttccat ctgccacgtt
     5101 ctgcttttgg gtctacaatc actgcttgtg ccccataaag cactgcataa tagacgataa
     5161 ggttattcgc aaaggattta ccaccaccca gcgaaccgac aaaggcagac gctaacgcat
     5221 tggttactga acccttaacc ccttgactgg caagagcagg tttcagatag acattgcgtc
     5281 cagtatctaa gctgtagcca acataaatcc cctcattttc acccagcatt tgagtagcac
     5341 caaaacctaa accagcgagg aaatcagagg tcacgtattg aatatagtca ttcatatatc
     5401 ttttgctggc aggtaaaaat tcttcatgta agcccagcat atccccaaaa ggtcgtacca
     5461 gtttcacgct caaatcatca tagaaatctt tcacttcatt acaacgacgt ttaagttcgt
     5521 caaggtcatt tgctgatacc cttacaacat aggacagctt atacatagat tccttgcttt
     5581 ggtctaaatt ggtttccagc tcgttcacac tttccagagc ttccgccaca ttagagctgg
     5641 tttcattatc actttgccaa gcgtggttat ccaagtcttt cagttctttc tttttattgc
     5701 ggacagtaga tagggcttta cgattcgcta caatttccac attcattgac gtatcaatcg
     5761 gaaaggtaaa ttgctgttgc tggtagtaga agatttcaga ggacgggaag tccagttcgc
     5821 cgacaatact gttgatggta aagtaagcta cataggcggt ttcgtcttcc tgctggattt
     5881 tcaaatatcg ctgtttttct tccaccagac agcgagtagg cttaatgagg tcataggttt
     5941 taatcagtgt ttcatgttcc agctttttct ttgatagagg atactcatac tcttcatagg
     6001 cagtgcctgt ctgtccgtaa aggtgttcaa tcagatagcc gaagtcatcc ttatctaatc
     6061 ttcggatttt gaaacgacgg gagattttat tttctaaaag cttttccatc ttctgaaaac
     6121 gcaggatttc atcattactc atactaacaa aatcgcccat cagcttatgg ttcacatcat
     6181 agacaaaatc agtaaaagca ttttttgctt caacggtaag acttttcata gaaaactcct
     6241 gatcgttgag aagcaactta aaaccgataa agaaacggta gtccacttga ttttcgccaa
     6301 tcatggatat taaagcgtct gtctgttggt cgattttgtc ataggcaacc gttttgagct
     6361 ttccagtgac ttcatttttg gaacgttctt gtgcagaaag tatgctggat tctgtactga
     6421 tttgtaaagc atgaatcttg ccatcacgat tttgtgcgat aagctgtctg aaagaatcat
     6481 gtacttgtat tttctgttcc ggacttagaa atgagtaatt gtagggaaca agctcatagt
     6541 aagcgtaaca ttccccatct ttattccaga ccagattgtt ttcaatatat ttaattggat
     6601 atgccataaa attcactcct aactgctgta atggcttctt gtggctggtt tctgccaagc
     6661 gttacttttt ttcctgcata ggtcagcttt cgtcgcagtg cataagcaat gacagacttc
     6721 aaaaatccat aaggcttttt accatcaaag gtttttgtag acataaacca tgtgaaagcc
     6781 acaggaatcc caaagtattt gagaaatgct ccctctatca tagaaagagg gggcaagttg
     6841 ccaagtatca taactgcaaa gagtgacacg acaaaccatg tcatttgcgt aaaggttatg
     6901 ggaaacggaa gtctaaagtc attgatggag tacaacactt tctcaacaga ccagatactg
     6961 gtatagcttc gtattttctt catgtaatca atcctttcat aaaaatgggg atagtctttt
     7021 gactaccccg taaacaagaa aatctgccag taagaatgta ccgacagatt taatagacga
     7081 tttcaaaaat cccgtgattg gttgagataa acgttcccga aaggtctaaa tcccgaccat
     7141 aggattcata atcaatatag ttttgaagac tggctggcac ttcgcctaaa gcacccgttt
     7201 cttcaatgta gtagcgtgcc acgtcagaca tatcatcaca atcggaatga atgataatat
     7261 cttcttgatg ttcgcttagt tcttcaatac ttgaaaaatg agtgagcaga gcagatagct
     7321 ccgattgtag ttcttctggc aactccgata ccatttccca taggcgattg agttcgctaa
     7381 tagaagtgta ttcgtcaacc gtaaagggta actcgtaatc atgaatggcg tattcctcat
     7441 attcatcatt caagccgatt ttctctttga cttcctcaaa gtcaatggga aaggtaaacc
     7501 acgcaccgac caattcgccc tcattgtatt tgcctaaatt cgcaatatag acttgcatat
     7561 cgtccatata ttcacgtcct ttctttgtag agattcaaaa atccctaccg cacttcgttt
     7621 ggtgtaccat tcctttgcgg aacataagaa aaccacttat attccacaaa agaacggttt
     7681 tatttaagca ccaataatgc gattgaatag ctctagtaaa atgtctttta ctccagcagc
     7741 gttgaagact aagccaaccg caataatcgc aataattaaa aagccaatca gtttgctaaa
     7801 ctcacgcttg aagccaagat acaagccaat cacaacgatt gctaaaagca ccagtgattg
     7861 agcgtttgat agaaaccagt tatatatgga taggtaagtt cctgatatta taaatatctc
     7921 aaacttttcc ccccattcac acggagcgtg cgactttcac cgcactccgc gttccatcgt
     7981 gtttatattg aaaagtattt atgtagtaga cctatatcaa gtttaattct tgattgccag
     8041 ctaatactct atatttattt agtcttatga gctggacttt atttagcttc aatagagcta
     8101 tatatttttg tattgtttct tctgttttag cgtgtattag acggtgtacc gcctctttta
     8161 ccaaaataag atttttataa tcgtcatttc caccagctcc agttggtaca atatgatgac
     8221 aatgtatttc attagcttgt tgaaattctt ccagagttat agcacatttt ccatgttgag
     8281 cagaaaatag cgaaattcga ttgtctgcgt attctattgt gcgattttcc aaagataatt
     8341 tcattaactg gtacatcagt aatttatcaa cacttagatt ggtgtggata aattttcttc
     8401 cctctagagt gtagcaattc gctaaataca cctttgccat tggttttctg tgttggatat
     8461 atccaacagg gaaaataggt tcgccctcgc cagcaacata ccgaagcatt ttagatttac
     8521 catatcttgt tgcctccttt gtcgtaagtt gtctgcctgt ttttctcaat ctgccccttt
     8581 tgtttgtacc taatctgtta gtgaagatag ccattacagc tctattcagt ttcatacaat
     8641 ccagatttac attcgttgca atctgataat aattttgcat acctaatacc attgaattat
     8701 aaagtcttgt ttcttctaat tccgttttcc cttttgaggg agaagcaatt ttctttgcct
     8761 gtgataccag cttgtctttt tggtttttga attgtttatc acttatatgt gatttaacaa
     8821 ctaacttctt tcttctcggt atcattttaa tcttgaaacc aagaaagtcg gagtatcttt
     8881 ttctaacatt tacaattctt gttttttctt gtgatacttc caattttaat cgttgttcaa
     8941 tccattgagt gattgctatt ttggcttttt cagcactttc cttgtagcgg cagaaaactc
     9001 taaaatcatc tgcataacga accatataca tttcctttaa ctttgttttc ttcatttctt
     9061 tgtaagcgtt gcttttacat gggctaccgc ttttattgat atgaaccaca tagttttttg
     9121 taacaggatt ttcctgccat tggctttcta cccaatggtc taactcatta agaacaatgt
     9181 ttgctagtag tggggatatt attccccctt gcggagttcc tttctctgga tatgttattg
     9241 taccgttagt cattttgatt ggagctttta atatcctttt taaaatgaat atcaagtgct
     9301 tgtcccgtat tcccatagcc catatctgtt ttatcagctt ggaatggttc acgttgtcaa
     9361 agaaaccctt aatatcaaat tcaatcacat aatgtagctg gctgatttgt aggcggttat
     9421 aggttgcttt tattgcattt tcaactgacc tattaggtct aaaaccatag ctgttttcac
     9481 taaatttagc ttcgcagatt ggttctaata cttgttttat acattgttgt ataagcctgt
     9541 cccacataca aggaatacct agaggtctgg ttttaccatt tggttttggt atttcttttc
     9601 gcctcactgg cttaggtcga tacccatgtg gactgcctgc aacaatgtat cttacttttt
     9661 tgacaactgt ttctgctggc aatttgccaa tatctttaat tattgtaccg tctgttcctg
     9721 ctgttttact gcctgcattt gctttgatat ttcgatatgc cagtaatata ttttctctcg
     9781 ataggataat atccataaga tgagtgaaaa tatctccatt cttacttttg gcatataaat
     9841 catcaaaggt gttttgcatt tcgtaatatt cagcgtgtct taaatcttct acacacaatg
     9901 tttcattttt atttttaggc ataaggcttc actcctttac ttgaaattga gtgagctttt
     9961 ttatcttact cgtaatcctt attgaaacaa cataatactt ttctaatata tacacgactt
    10021 gagggcattc ctccattctc attacaagaa tttcttaggt tcgccctctg cttttcagca
    10081 ttagttcaac tgcttattgt tcttcgtcag tttattctct tgatgagaac ccaatacctt
    10141 tcctcgttcc gataatctta tctatacata tatatcctta ggtcactagc tataagcctg
    10201 ctggcttgat accgcctgta acgttatatg gcatttcata acctatattc ttactttgcc
    10261 acaccagcta cgatttctcg tacctttgca tttctacaaa gttcaaattt agacccgtac
    10321 attcgctgtt tcgtcagttc cttatagaac attctaacca tagatatttt atagaccccc
    10381 gacctgttgg atacgctacc cacctctgga tagttttcga tagaagatat atgttcatca
    10441 actattacgg tatctttcag ccgtctttac cgagcttcaa acattctgag tttcctcata
    10501 caatgcttgt cggaggctca gggcaggcgt ttcagagcgt tacttcatca ttccacctgt
    10561 cagattatca gttctcctaa taaccaaact ttttcgttct tttattagtg ccttagcttt
    10621 tcactaagga acgagtcgca caaaggtttt gtccaaaatt cataaaaatg ttctcctctc
    10681 tatattcaat gaatttgtat ttgagttatt tttttgttgt tatcacgtcc tgttctttta
    10741 ctgactgttg cttcaaaatc tgcttgtgtc ggtctgtcag tttcgcatgg tcgagaatgt
    10801 cttttacaac ctgcgtctgg ttgatttcat caagtttaat cgcaaccttt aaggtcgggg
    10861 caacttgatg agatagccag ttcagcgtcc tttggaagga gtaaggctct ggttttgtgg
    10921 ttagttttaa tcgttcacga ttgttcccaa taaaccaagc ccattcttca ttcagtttcc
    10981 aatcagaacg aggtttggaa tcgtctttat ctacaaaacg gatataccga ttgataattt
    11041 taaaggcggt atgctctgga ttgtcataga cgagtaaatc acggactgca taataggcac
    11101 gctcattttt caatcgaatc tcaaaacggt tttttacttc tgcgtcttca atgggaatat
    11161 catttttctt gtactgctcg tagtcctttt catagataca gaaataaact tcactttgta
    11221 atgaaccgat atagagggtg tttcccatac attccttttc ctctttgcgt accagttcgc
    11281 cactgcgata gcttttaaaa ctgcggaaga cggagataca ttcttcctgt tggcactttt
    11341 cagtgagtac agggatattt aaaatccctg tcttatcgtt aatggcaagg tcaaggcgtt
    11401 tcatcacacc gccagccacc aaaacgtcca taaagaactc ataccagctt ctttgttgtg
    11461 ccagaagata gctttcaaat tgtctgcacc cacgaccttt caattccacc agaactcctt
    11521 tgtccagttc atgggagcaa aggacgaata tgtcgcctaa agcataatgc tctgaataag
    11581 aatagaaacc atagtcctca tgaagaaaat aggacagttt cagttgtaag atgttttcga
    11641 ccacctgctg tacgtctgtt gtcggaaagc gaattcttac ataatcaaac agcatttcaa
    11701 ggggagcgtc gggattgaag cgttccagag cttcccaaag ggactgctgt aaatcctctg
    11761 atggcttgac ttttcctgtt tcaatatcgc ttagatactg ccttgtaata ccagtcgcaa
    11821 cagctaaacg gttttgagat agtccataag ccaagcgttt ttcttttaaa tgctgtaacc
    11881 aagtttgttc attcagtaaa aatccctcca atcaaaaagg cgtatgtcaa cttttaaagc
    11941 ccatttgaca tacgctgaaa ttttgtaaat cccttgtaac caaaggattt tctaatgttt
    12001 ttttgactgt ttcctgtcga tttgtacccc cctgttagat accgaggggt taagtgctgg
    12061 cgtggctatt gccacaccag ccagcaagat cagtccacac ctgcgacttc cgcttcgcac
    12121 gtcgcctgcg tggactgtct gctgttggat aactttttaa tttcctccaa gaaatcatat
    12181 ccttttggta caaggggagt ataaaactct gatatgacac ttgttcctac atcaacatag
    12241 ccacgacctt tgattcgctt taagaagaaa tccttttgta cgtcactgcc aaacatcatg
    12301 ccatagccca tttcagacat acgacctaaa gccactctga aattaaactg atcacggatt
    12361 ccgtcgccta aatattttgc gtctggacgt tgacaagcca gtattagaaa gaagccagct
    12421 tgacgaccta acatgacaat ctgtttcagc ttattcataa ctgcggtgtt ttcttttgtt
    12481 cccagcattt ccatgaaagc gacgtattca tcaaagatta agaagtgtgc cgggagacct
    12541 aagtaagcat aatttttgcc agtcttatag ttcttcatct gcttcatttc ctcactacgt
    12601 ttcatcattt cttcatagaa tgtttcaatg caagaaagca agtcttcttt tctatagtag
    12661 acatttgcca tcacagaacc taagtccgca aggtcagcat ttttcgggtc aagaatatac
    12721 agttttgaat ctgtatgaag caaggcttca atcagtgtca gtataaagta agttttaccg
    12781 ccacctgtac caccagcaat caacatatga gggagcttat catattccca ccatacgttt
    12841 ttcattaagc gaagtttacc atctttagct tctacttcat caatagaaat acgactggct
    12901 atggtgtcat agagcaaagt atattccaca taggaatcct ttaactcttt atccgtcagc
    12961 tcacagtaca agccactctc taatttcttt tccaagtgta agagttggtc ttgatatttt
    13021 cccagcgtga tttccacccg tatctgtatc aagccatttt taagtcgata atacatttta
    13081 gggaagtagg ttatcttttc ctttgtacga ccagcactat ctttaaagaa accctctgtt
    13141 ttgacctgtt cagattcata ccacttgttt tcaagtatca tctttgccag tttttgacgg
    13201 tggtaaagtt gtttaaccgt atcatagcga acccgtttga atacaaacgc taccagcaag
    13261 cagataagaa ttgcgacact gaaactgata attaaatagg gaatgtcaat cttatctgct
    13321 tgtgataggt taaaatcctg ccagttgatc tgctggattg tcttcacatg aaacagtccg
    13381 acaaccagca ggaaaacagg caggagtgac gctatcgtaa aatgaaagac taaatcttta
    13441 ccagatgggc gaatcctttt accacgctgt ttcatgcgaa aaagtctcct ttctacctag
    13501 cgactatttg tcttgtgtcg gttctttctt tgcttgtggt tgagctttga atgaactaga
    13561 atcctttgtc agcacaatat cgtctgcctt gatataccag tcaacatctg ctccttgata
    13621 ggtggcagta gcaacggtgt ccgcaatggg attgataagt tccacccgtg cgttataatc
    13681 aaactctttc aaaggcacgc tggcaggaat acttacttga atcatgcgtc cttgtccttt
    13741 ggattttaag tcataggtac gttccttgat ttcatctgaa accgacccgt cttcattttg
    13801 gattctcact tcacgacgta gagcagagaa tttcaattct ccaaaagtcg tgtctttatc
    13861 taatacaatg ccatttgcta atctcatcat ttttcctctc tttctttatt cttttatcat
    13921 gtcgtcagca tgtaaaaggt aatttgtaaa accacgagtg ccgattttgt agccctctgc
    13981 ggtaatacgt ggattgacta acttcacacg ttcctcaaag ccgaaatgtt tttcgccagc
    14041 ttcagcagga agcaccacca caatatcatc tgctctttga acatcagaat agagattata
    14101 gcttcttgat aagacagtta gccgtccgtt gattcttcgc tgaacgactt tatcctcgcc
    14161 agcaaattct aaattgccga atgttttttc catgttggga atcacaaatt taagttccat
    14221 atttttacct atcctttctt ttttattggc tgaatgaatg tttgatggtc ttaaagagtg
    14281 gggaacgacc ttttgattct tgattttttg ttttcataag ttcacttcct ttcaaaatcg
    14341 ggtaaaaaaa tagacacctc attttttgaa gtgtctacct attaaatatt caaattttat
    14401 tggaagtatc tttatatctt cacttttcaa ggataaatcg tcgtatcaaa gctcattcat
    14461 aagtagtaaa ttagtagtaa attgagtggt tttgaccttg ataaagtgtg ataagtccag
    14521 tttttatgcg gataactaga tttttatgct atttttctct taaaaaatat cattatttgt
    14581 ggtatggagc ccccgcgttg atccggaagg cacggtagat ttgctcgacg aggatcaggc
    14641 gcatcaattg atgtggatac gtcattttgc caaaagagat ctgagcattg cttcttttca
    14701 tgacggcttc gctgagtcct aaagaaccgc cgataacaaa aacaaggtgg ctttttcctt
    14761 ggatacctaa ttggtcgatt tgtttagcaa aagattcgct tgtgggattt ttcccttcaa
    14821 ttgctaagcc atagacgtat tcttgttctt tgattttcgc taagatccgt tctccttctt
    14881 tttcttttac ttgtctcatt tgcgcttcgc ttaaattctc cggggctttt tcgtcaggaa
    14941 cttcgatcaa ttcgattttc gcataggcat tcagccgttt gacatactca ttgatacctt
    15001 ggatcaagta tttttctttt aattttccta ctgaaataat tttgatattc atgttttctc
    15061 tcctttgatc ttcacgctca ctttgtttat tataatcaat tttttcgtct atatgtttat
    15121 tttgactatt tttgttaaaa agatttcccc aacagttatg cacaacatcc acaggtttat
    15181 ccacagaaaa cagcgttgtt ttaccttatt tcatcacatt cattaggcaa acatacgttt
    15241 tataagaatt tatccacttt atacacaggc tgtggatatg ttttatccct tatttttcca
    15301 atcctatccg cgtagtttta cacaatatct gtggaaaaag aaaatcaaat attttcatct
    15361 gaaaacaatg atttttccta aagtttttca taaaaagttc aaagtctcaa agtagactaa
    15421 agtcaacaaa taagactata ctaacagtag ataaatgaat gtaattggag gaggaacaca
    15481 tggatagaaa aaatgtgaca ccaaagatga aaaaaaataa aaataatagc ctctggcgta
    15541 agttgggtct ggggcttgtt ggagggatcg ttggaggact actgacagct ggcatttttt
    15601 atgcagcgat gggcactggc aacaatagtt ctactgctac aagcggcaat caaaattctg
    15661 ccggtgaaac agttgtggaa aatgtcaaag tcaatgtaga ttctgatatc accaaagcgg
    15721 tagataaagt tcaagacgct gtcgtttctg ttatcaactt acaaagtcaa aaccaatcaa
    15781 gcggttttgg cggattattc gggcagcaag aagaaagtag cagctcagat gatagtaatt
    15841 tggaagctta tagtgaagga agcggcgtaa tctacaaaaa agacgggaat actgcttatg
    15901 tagtcacaaa caatcacgta gtagatggac agcaaggttt ggaagtcatg atgaaagacg
    15961 gtacgaaggt aaaagctgaa ttagttggaa cagatgccta ttcagattta gcggtgttga
    16021 agatcaattc agacaaagtc gaaacggttg cctcttttgg ggactcaagt gctttgaagg
    16081 ttggtgaacc tgcgattgcg attggttccc cattaggatc tgaatatgcg aattctgtca
    16141 cttcaggaat catttcttcc ttgaaccgac aagtaacaag tacgaatgaa tcgaacgaaa
    16201 cagtcaatat caatgcgatc caaacagatg cagcgatcaa cccgggaaat tctggtggtc
    16261 ctttagtgaa tatcgaagga caagttatcg gtatcaactc aagtaagatc gcaagtactt
    16321 ctgaatcgac ttctaatgta agcgtagaag gaatgggatt tgccatccca agtaacgatg
    16381 tagtgaatat catcaaccaa ctggaaaaag acggaaaagt gacacgtcca gctctaggta
    16441 tcacaatggt cgacttatct gccgtttcta ctcagcaaca agaacaaatc ttgaagattc
    16501 cttcatctgt aactaacggt gtcatcgtga caaatgtcca aacagctaca ccggcagaaa
    16561 aagctgggtt gaaacaatac gacgtcatta ctaagatcga tgacaaagaa gtttcttctg
    16621 gtgtagaact tcaatctgct ttgtataaga aaaaagtggg agattctgtc aaaatcactt
    16681 actatgaagg ctcgaaagaa aaaacaacta ctgttgaatt gacgatcgat caatcggcat
    16741 taaaacaaaa tcaaaatagt gaaaattaat aaaaaagagg ttgtgacaaa gacttgtcac
    16801 aacctcttta ttttggaaat acttctccga ctacggcatc ctcaggcaat actaagattc
    16861 cttttttttc tggagcattt ggcaaatgca attcacgagc agaacaaatc attccatagc
    16921 tggctacccc tcgcaaagtt cctggccaga tcatcattcc atctggcatc atggctcctg
    16981 gttttgcaac gacaactttt tgtccagctt cgatatttgg tgcaccgcaa acaatttgca
    17041 atactgtccc attgtccact tctgtctcag taatcgataa atggtcagag tcaggatgcg
    17101 ggacgcatgt ttttacatac ccgacaacga tcttcggttc atgatcagct tcaagctgtt
    17161 cttcgaatcc tgcttcttta atcaatcgat tcaatttcgc taattcttca tctgttaagc
    17221 ttacttggcc gttcccttca attgttagat gatcggagat atggaaaaag ttccaagcaa
    17281 caacttgacc ttgttcatct ttgatccgag caacattcca tttacgttcg acagtgtttt
    17341 ctgttccttt atcgtcggct atgataacca tcaaagtgtc tccgacgtgt gctttgttat
    17401 aagcaaaaat catgttcttt cctccattat cctttgctga gatttatctt aacaaaaaat
    17461 tgcattcact gcatgagatt tttttcatgc tttatttccg aagaagcttc ttctggaaca
    17521 ccatttattc gtaacaaggg actgtgacaa gacttttgtc acagtccctt tactgtttga
    17581 ttaaaaaggt tatacaagtt ccacttgctt aattactcgg ctgttcttct tcgtcagaaa
    17641 cgatcaagac atcacaaggt gaatgacgta tgacgtagct ggctacactt cctgtcataa
    17701 agcgttcgac tgcatttagt ccagactgcc caaccatgat tagatcgatt gcatattttt
    17761 ttggcaattt gactgccatg gattcacgaa cagagccgta tgcaataatt ccttctatat
    17821 ttgtgtaatc aacagatttt ccatattctt tacattcttc gatcatttgc ttggctgttt
    17881 cagtttgctg atcgactaga ttttgatcaa taggagcata gcccataaaa ttataaaatt
    17941 gctgatcgat tacgtttgct acatatactt tcccattatt acggcgagcc acttctaacg
    18001 cttttttgaa agcagcaagt gcttgttcac taccgtcaat acctactaaa atgttcttat
    18061 attcttgatt catcttgacc cctcctaaca gatgtcagcc ttttattaca ctttctagga
    18121 acgattcgat ttcctgtttt gttttacgat ctttattcac tagacgacct aattcttttc
    18181 cgtcttggat cacgacaaaa cttgggatac caaaaatgtt ccattcagcg gctacatcta
    18241 tgtacttgtc acggtctact tcaataaatt gccaatcaga aaagtcattt tcgatttcag
    18301 gcatttgcgg tttgataaaa cggcagtctc cgcaccagcc ggcagtaaag aaaaatacac
    18361 tttttccagt ttctacgtag cctgctaatt cttctaaact atttgggata atcattcttt
    18421 ttcctctact ttctgtttat gtatttttca attaaattat acacctttct ttcgaactaa
    18481 caaacattga tcctcttttt ataaaaaaaa tctgacaaaa ggttgctttt gccagatttt
    18541 atagtttcac tatcgcgtta tttcccgtat acgatcgtgt tcactgttga tttatctaaa
    18601 cctttcaatg tttgaatcaa catttctcgt gcagcttcaa aatcgcgaat actgaacatc
    18661 gtttgatgcg tatggatata acgtcccact acgccaatca ctgtacttgg gattccttca
    18721 ttttgtgtat gagcagcacc tgcatctgta ccgccttttg aaacaaagta ttgataaggg
    18781 atgttgtttg tttcagcgat atctagcaag tactcacgca aacgaggaag catgatcaaa
    18841 cctggatctt ggatacgcat caatgtacct tctcctaaat gtccgaatgt gccatttttc
    18901 gtcactgtgt catctgctgc agagcagtct actgcaaaga acagatctgg cttgaattta
    18961 ttgacagaag ctttagaacc acgcaagcct acttcttctt ggacattcgc gcctgcaatc
    19021 aatgtatgac ctaatgtttc attttgtaac gcttctaaag cttctaatac cattgtacag
    19081 ccatagcggt tgtcccacga tttgctgatg atattcttcc cgttggctgt tttgatcgtt
    19141 tcagcgtaag gaacgattgt gtctcctgga agaacaccgt aactcattgc ctcttctctt
    19201 gattcaaacc cagcatcgaa caaaatatct gttacttcta ctgatttttg tccgcttgtt
    19261 cctcgtaata aatgtggggg aacggaagaa gaaatacatg gataattctt accagtagag
    19321 gttttaagtg taaaacgttg agcagaaaca acgtaagggt tccatccgcc tagaggtacg
    19381 actttaaaaa gcccgttttc ttggatttgt gtcagcatga atccgacttc atccatatgt
    19441 gctgcgacca ttacacgagg tgcatccgct tcttcatggt ggcgcagccc aaaaatgccg
    19501 cccaaaccat ctaactgtaa ctcatctacc agcggttcca tatgactttc catataggcg
    19561 cgaacgtctt gttcaaaacc gcttgttcct tgtaattctg ttaactcttt gattcgttga
    19621 aatgtttttt cttccatatg ctattcgtct aaatttcaga ctaaactcct ctcagactta
    19681 tattgatctg cggataaaat atgatttttt ctgcagaccg atcgtattta ttatagctta
    19741 ttttttctgg tagcggttat ttatttcaaa gaaaatatga gagtgtggta aaataaacac
    19801 aaagagtagc aagtgagaag aaggaggtat atccacatgt atgaagaaag cgaattaagc
    19861 tattttaaag gcgggttagc cgttgggatc ggtctaggat tattgagtgg ggtagcttca
    19921 acgatttggt atcatcgcaa aaaagtgtta tcagcagacc aagtgcttga aaatatcaaa
    19981 acatcttttt taaaagaagg cgcaatcgaa ggtgcttgga tctcttttga aaaagaacct
    20041 attcggaaat tcgccgttcg gtcgagtgct tatcggggtg ggctttcccg gatagaagat
    20101 ggcgaaactg tttattatga atttttagca gatgctcata caggaaccgt attagacatt
    20161 tcaagaaaaa agggcgcaat agattaatac gtcaaaaaaa tgaaataacg aatacgcacc
    20221 cactatctaa aagagaggta tcctatcctt cttttatagt gggtgatttt tataaaaata
    20281 gggctatttc ctcttggaat agctagtatt tgtagttatt atacggtatt cttgaaataa
    20341 gattagtttt attatataag gagagtgaca aatggaatca cgtgaaaaaa ttgccgtgct
    20401 cattccttgt tataacgaag aggcgacaat ttcaactgtt attgaagatt ttaaaaggga
    20461 attaccagaa gcagatattt atgtctatga taataattcg accgatcgaa catacgaatt
    20521 ggccgttcaa ggcggagcaa tcgtaaagaa agaaccacgt caaggaaaag ggaatgtaat
    20581 ccgtcaaatg ttttttgata ttgatgccga ttattatttg atggtagatg gtgatgatac
    20641 ttatcctgca gaagctgcac atggtttgct tgagaaatta cgttctggcg aagcagatat
    20701 ggtcattggc gatcgtctat caaatggaac ctattttgat gaaaataagc gtcctttcca
    20761 tgattttggg aataatctgg ttaaaaatac aattacttac ttatataaaa caaaaattcg
    20821 cgatgtcatg accggctatc gaggattcaa tcgaattttt gtcaaaagct tccctattat
    20881 gagttctggt tttcaaatcg aaacagaatt gacgattcat gctttagata aaaaattcaa
    20941 gttcgttgag attccaatcg attatcgcga tcgtcctgaa ggtagcgaat caaaattgaa
    21001 tacattctct gacggattca aagtgatcat gatgatcatc aaaatgtgca aagattataa
    21061 acctttgctt ttctttggga tctggaccat cttcttcttt ttatttggat tggtcatggg
    21121 gattcctgtg attcgtgaat atatgctgac aagctttatt acacgcatcc cttctgcgat
    21181 tttaagtact ggattgatga ctttagccct tctttcatta gtaacagggt tgattttgga
    21241 tacggtcgtc acaaatgcca agaaagaata tgaattgaat ttataccatg tctaccacga
    21301 atatcaaaat aaagaaatga agtaggaggc cttttttatg cataagaaaa aggagaatct
    21361 atttcctact ttttttgcaa ctttatttgc gtcaggtttt gcttttttta cttcttatgg
    21421 tctaggaact ttgaatccaa cagtaatcat tggtttagcc atggttttgc tacttgcttt
    21481 taagcaatat ggaatagagt tgttttcaat aaaaaacaaa gttgcagcta ttgcccgatt
    21541 gtttttttct gcagcttttg ctcttacact ttctattggc agacagatac aatttcagta
    21601 tgtctcagca aactatacaa agacaacgat tgctgatgga agtatttttt actttcttgt
    21661 ttatttctcc atttttgtgt attctttttt atttttacaa tcgatttatt tatcaatgcc
    21721 aaaactggaa acatttttta ctgctcaaaa cacacaggct ttctcttcaa gaaaatattt
    21781 tttattcttt actgtctttt tagttctgtg ttggcttcct tatctgttgg cattgtatcc
    21841 tggagtggtt ttgcctgatt cgttaagttc ggtcgcccaa tctatgggtg atgttcctat
    21901 aagcaatcat catccagtct tattcacctt actggtcaag ctatttgttc atgatttacc
    21961 aactgatacg atcaatcacg gtgtattttt attttcgatt tttcaaacat tgataacagc
    22021 ttcagcgata agttattctc tttgctggct gttaaaaaga aacatccatt tttttccggt
    22081 actcattagt ttatgctatt ttgcatttgc accggttttt ccaatctacg ccttgaacat
    22141 gcaaaaagat actttgttct ctatcgcttg ctttttattt tccttagcgg tttttgactt
    22201 tatttttcaa cgagaaaagc caacgattct caatcagatt tacgtaatca tccttgcttt
    22261 actgaccacc tatctaagaa ataatgggat ttatgtagta accggcacag gtttgatttg
    22321 cggcttgatt attttgaaac aaaaaaggta tcaagcattc tttcaagtgg tgctgggtta
    22381 tacagtgatt gctggactac tcatccaccc gttgatggct cgttatgcta ttcctacaga
    22441 aactgctgaa caattaggtg ttcctttgca gcaaatttct agaactattg ttatggatgg
    22501 tgacttgtct aagaaagata aagcattcat gaatcagcta ctcccgataa aagattatga
    22561 agcgtattct cctagtcttg cagactcaat caagtggcat aaaaatttta acaatgagtt
    22621 tttgaataca cataaaaaag aatttctatc actatgggca agaaatttac caagtaatat
    22681 aaaaatctat acagatgctt atattttaga aacgttcggg ttctgggtac ctggtacaaa
    22741 aaatagttac ggctttttag atactcgtgt aaacacgaat aattatggta tgaagcaagt
    22801 agatttgatt gaacatctta ctggattgtc aggaatgaaa aaaatcatcg atgctcgtga
    22861 tttctttggt tccggcacat tactgtggtt agctatcggc agtgcacttt tagcaatctt
    22921 taagaaaaaa tatcggtatt tactcctttt tcttcccggt atgcttgttt ttgctactat
    22981 tatgattgct actcctgtgg cttttagttt acggtatgtg tttgttttag cattaggtct
    23041 acctgtttat atcctctctc cttttttact agataaaaga gaaaaaatga aaacagatat
    23101 aggagatact catgaaacta tttaaaaaga tattttttgc ttttgttctc ttgatcgcta
    23161 gctattgcgt actactgaca attacttttt tcattccgca gcactctatt gaatcgaatg
    23221 cagaaaaatc tctacagatg gttgaaaaag aaggaatgta tccttctatc aatcaaggaa
    23281 atatgctagg cactcggtta gataatttta ctgatcattt gatgatacga aaaacaaaag
    23341 cagatcctga attgaactcc ttggagaacg cgatgtctat ggcggattat ccccgttatt
    23401 ggcatggtta tcagcttttt ttacgacctt tactgctagg gatgagtttg gggagtattc
    23461 gaatgattta cgcagccatt ctttttctgc tgattggatt gacgagctac tttttaacca
    23521 agtgtagcga catctattta acgacagcct tgttgatttc gcttgctata ggaaatgctg
    23581 caaccttttt cttcagtatg caattttcaa atatttggat tttgacttta ttgtctgtat
    23641 tactattttt aacaaaacca caactctcca acaccaacca acaattattc ttgtatttct
    23701 ttgtcattgg ttctttagct aacttttttg acttgttgac aacaccggtc atctcttggg
    23761 ggatacctgt gatcattatt tattatgtaa caaataagta tttaatggat aaacgcagtt
    23821 cattgagcaa acaagtaggc agttttgttt ttaccggtat cttttgggga ttaggctatg
    23881 gtttgacatg ggtggctaaa tggatcctat catcgatcat attgaacaaa aacattgtaa
    23941 aagatgcaat caaccaaatc ctctttcgaa cggaaggaaa cgacaaatac ccgttgcatc
    24001 gattagagat gctgaaaagc aatattagat tgatgtatcc aaaagtagca atattattgc
    24061 tattgattac ttgtcttgtt tttctctatt ttgcttatcg taaacgaaat agctggcgtc
    24121 cgttccaact tctagttttg tttgttttag ctgctgtaac gccgtatatt tggtacaata
    24181 ttttagctaa tcactcacag attcattatt ggtttacgta tcgtacgcaa atcatcacaa
    24241 gttttgctat acttagtgct ttcgcctttt tgatccctcc tgagaaatca aaagacgaac
    24301 taaattactt ttaagaatac cgatgtacta attcattttt aaacataaaa actccagctg
    24361 tcagagatag ccggag
//
LOCUS       DAZKNO010000024        24368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000024 DAZKNO010000000
VERSION     DAZKNO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.024"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..783)
                     /locus_tag="ACG8MY_000716"
     CDS             complement(<1..783)
                     /locus_tag="ACG8MY_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0429938.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNE"
     gene            complement(1005..2948)
                     /gene="thrS"
                     /locus_tag="ACG8MY_000717"
     CDS             complement(1005..2948)
                     /gene="thrS"
                     /locus_tag="ACG8MY_000717"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0429939.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSRENK"
     gene            complement(3285..3464)
                     /locus_tag="ACG8MY_000718"
     CDS             complement(3285..3464)
                     /locus_tag="ACG8MY_000718"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0429940.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3511..5334)
                     /locus_tag="ACG8MY_000719"
     CDS             complement(3511..5334)
                     /locus_tag="ACG8MY_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0429941.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHAHSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5612..7891
                     /gene="helD"
                     /locus_tag="ACG8MY_000720"
     CDS             5612..7891
                     /gene="helD"
                     /locus_tag="ACG8MY_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0429942.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETEIDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(7984..8631)
                     /locus_tag="ACG8MY_000721"
     CDS             complement(7984..8631)
                     /locus_tag="ACG8MY_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429943.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8810..9529)
                     /locus_tag="ACG8MY_000722"
     CDS             complement(8810..9529)
                     /locus_tag="ACG8MY_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0429944.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9660..10811)
                     /locus_tag="ACG8MY_000723"
     CDS             complement(9660..10811)
                     /locus_tag="ACG8MY_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0429945.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11028..11768)
                     /locus_tag="ACG8MY_000724"
     CDS             complement(11028..11768)
                     /locus_tag="ACG8MY_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0429946.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11772..12128)
                     /gene="rsfS"
                     /locus_tag="ACG8MY_000725"
     CDS             complement(11772..12128)
                     /gene="rsfS"
                     /locus_tag="ACG8MY_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0429947.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(12152..12745)
                     /gene="yqeK"
                     /locus_tag="ACG8MY_000726"
     CDS             complement(12152..12745)
                     /gene="yqeK"
                     /locus_tag="ACG8MY_000726"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0429948.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12729..13379)
                     /locus_tag="ACG8MY_000727"
     CDS             complement(12729..13379)
                     /locus_tag="ACG8MY_000727"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0429949.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13389..13700)
                     /gene="yhbY"
                     /locus_tag="ACG8MY_000728"
     CDS             complement(13389..13700)
                     /gene="yhbY"
                     /locus_tag="ACG8MY_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0429950.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13716..14825)
                     /gene="yqeH"
                     /locus_tag="ACG8MY_000729"
     CDS             complement(13716..14825)
                     /gene="yqeH"
                     /locus_tag="ACG8MY_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0429951.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14822..15349)
                     /locus_tag="ACG8MY_000730"
     CDS             complement(14822..15349)
                     /locus_tag="ACG8MY_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0429952.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15576..16361)
                     /locus_tag="ACG8MY_000731"
     CDS             complement(15576..16361)
                     /locus_tag="ACG8MY_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0429953.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(16354..17223)
                     /gene="accD"
                     /locus_tag="ACG8MY_000732"
     CDS             complement(16354..17223)
                     /gene="accD"
                     /locus_tag="ACG8MY_000732"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0429954.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17224..18603)
                     /locus_tag="ACG8MY_000733"
     CDS             complement(17224..18603)
                     /locus_tag="ACG8MY_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0429955.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFFSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18611..19030)
                     /gene="fabZ"
                     /locus_tag="ACG8MY_000734"
     CDS             complement(18611..19030)
                     /gene="fabZ"
                     /locus_tag="ACG8MY_000734"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0429956.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19055..19531)
                     /gene="accB"
                     /locus_tag="ACG8MY_000735"
     CDS             complement(19055..19531)
                     /gene="accB"
                     /locus_tag="ACG8MY_000735"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0429957.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19534..20769)
                     /gene="fabF"
                     /locus_tag="ACG8MY_000736"
     CDS             complement(19534..20769)
                     /gene="fabF"
                     /locus_tag="ACG8MY_000736"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0429958.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20788..21525)
                     /gene="fabG"
                     /locus_tag="ACG8MY_000737"
     CDS             complement(20788..21525)
                     /gene="fabG"
                     /locus_tag="ACG8MY_000737"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0429959.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21537..22454)
                     /gene="fabD"
                     /locus_tag="ACG8MY_000738"
     CDS             complement(21537..22454)
                     /gene="fabD"
                     /locus_tag="ACG8MY_000738"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0429960.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22561..22785)
                     /locus_tag="ACG8MY_000739"
     CDS             complement(22561..22785)
                     /locus_tag="ACG8MY_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0429961.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22838..23800)
                     /locus_tag="ACG8MY_000740"
     CDS             complement(22838..23800)
                     /locus_tag="ACG8MY_000740"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0429962.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23810..24250)
                     /locus_tag="ACG8MY_000741"
     CDS             complement(23810..24250)
                     /locus_tag="ACG8MY_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0429963.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6672 a         5419 c         4246 g         8031 t
ORIGIN      
        1 ttcgttttta gccatataga aataaaaaat gatatcgatc gtagcaaatt gggccaaaag
       61 tgaattagtt gccgcacttc gattcgtgat ttcttttggt ctagatgttt gtacgagtac
      121 atcggctatc ttgctaagcg ggttatttcc aaattgcgtc aacgcaatga tttcgatatt
      181 catgcttttc gccagctcgg ctaatgcgac tacatctgca cttctacctg agtttgaaac
      241 aagccaaagc actttctttt tctcattgct gacaagttgc gggagcaaga cgtggatatc
      301 tttttcaaaa atgattggct taccgacacg agaccatttt tgcagaatat cttctactac
      361 taaagaagag gcgccaaccc catataaaaa aacagtctct gccgattcca aacactctac
      421 gactttttgg atcgtttctt cttctagaat atctactgtt tcttttatcg ctacttgtgc
      481 attgttacca agtttgtttt tgatagagta aaaggactca tttgcttcta tatcactaaa
      541 accttcatcg attccagtct gtagttctgt tgaaatcgtg attttgaagt ccgtcagact
      601 ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc gcactcaacc cggaagcttc
      661 tgctaattca ttagctgtca ttgtagggac cttctcaata ttaccgataa tgtagttggc
      721 caactttttt tctgctgaag aataatcttc catcatactt tcaatacgac taattacgct
      781 cataagttcc tccacccttt taatgatagt ctatgtaagt ataccatatc agacccttgt
      841 taacggtcgt gtactggata aaaaaacaaa aaaacaatcc gcacaatagg atatcttact
      901 ccatcaatcc cacaaattct attgtccatc tgtgggattg tggaatatct gttctctatt
      961 gtttggattg ttttttattt agtgcttttt tataggcagc ttatctattt gttttctctg
     1021 ctgtaatttt tgacttctgc ttccatagca tcaataaaga tattcaaatc ttctacagct
     1081 gtttctttac ttccataacg acgtacgttc actgttgcat cttccaattc cttgtctcca
     1141 acaaccaatt ggtaagggat cttttgtgtt tgagaggcac gtatcttata gcccattttt
     1201 tcattacgat catcaacttc tacacgcaac cccttcattt gcaaacgttc tttgatttca
     1261 taagcataat cgccatgtgc atcaacagaa acagggatga ttgttgcttg tattggggcc
     1321 aaccatgttg gaaaagcgcc tttgtatact tctgttaaat aagcaacaaa acgttccatt
     1381 gtagaaacga ttccgcggtg gataactact ggacgatgcg tattttcccc gtcttctcca
     1441 acatatgtca aatcaaagcg ctctggcaat aagaagtcta attgtatagt tgataatgtt
     1501 tcttctgtac caagagctgt tttgacttga acatctagtt ttggtccgta aaaggctgcc
     1561 tcgccttctg cttcaaagta atctagttct aattcatcca tagcggcttt cagcattgct
     1621 tgggcttttt cccacattgc gtcatcatca aaatatttat ctgtattgtt tggatcgcga
     1681 tagctcaagc ggaaacgata atctgtaata ttgaagtcag catatactgc caccatcaat
     1741 tcaagcgtac gtttaaattc atctttgatt tgatcaggac gaacaaatgt atgaccatca
     1801 ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag cacctgattt ttcatagcga
     1861 tgcatcattc ctaattcagc gatacgaatt ggtaattcac gataagaatg gatcgtgttt
     1921 ttgtagacca tcatgtgatg agggcagttc attggacgaa gaacaagcat ttcgccatct
     1981 cccatatcca ttggcgggaa catgtcttct tgataatgat cccagtgtcc agatgttttg
     2041 tacaactcaa catctcccat tattggtgta tatacatgtt ggtaacctag actaacttct
     2101 ttgtctacga tataacgttc gatcgtgcgt cggattgttg ccccttttgg aagccagaat
     2161 ggcaaaccag aacctacttc tggggaaacc atgaacaagt caagttcttt tcctagttta
     2221 cgatggtctc tttcttttgc ttcttcacgg agacggatat attctttcaa agctttcttg
     2281 tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat tgtttgagtt tccacgccaa
     2341 taagcacctg ccactgataa taatttaaat acttgtatac ggccagtgga tggaacatgt
     2401 ggtccacgac acaaatcgat gaattcgcct tgttgataag cagtaatcac ttcttcttct
     2461 ggcaattcac taatcagttc tactttgtac ggatcacttg caaaaatttc taatgcttcc
     2521 tgttttgaaa gaacacggcg ttcgattggc agattttctt tgacgatttt catcatttct
     2581 gcttcgattg ccggcaaatc ttctgcagtt acttggtttt gtccattatc tgtatcgtag
     2641 taaaaccctg aatcgatagc tggtccaacg ccaaagtgga tatttggata gaggcgacgc
     2701 atggcttggg ccatcaaatg agctgctgaa tgacggacta agccaagtgc atcttcatgg
     2761 tctggtgtga tgatctcaat acttgcatct tcctcgattg gacgagtcaa atcaacgagt
     2821 tttccgttca ctttaccagc taaagctttt tttgctaagc tttttgaaat gctttcagca
     2881 attgccaatg ttgttgttcc agactcaaat tctttgactg caccatctgg aaaagtaatt
     2941 tttatcataa tagtttcctc cttgaaatgt tttgattgat cagatcatca agctgaaaaa
     3001 caaaaaaatc ctagcatctc ttgaaaagag atactaggac gaaaagttcg tggttccacc
     3061 taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg gtccgaccgc ccttgcttca
     3121 acaagggttt cgaaagtggt catcctttgg ttttctcctg gaaaagtttc agcctagctt
     3181 ttcctctctt atcgaagact ctcaaattca gttggctttc tcatcaatca attatgtgtt
     3241 tatttaacca ctaataaata gaaaaatcaa gtgggaaact aaacttattt tttccattcc
     3301 ccatttacac cgtatgttcc ttttttcatc tcattgatga tgacatggat gtgttctttt
     3361 ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt tcatcatatt ttctaattgt
     3421 tccggactgc gaccttctac taattcgaca tgtacgaatg gcataatgct ctctcctctt
     3481 tctgatcgtt cttttttcaa ctcgattcta tcatactatg tttgtcttgg gtactgaatc
     3541 aggaaaaatg gctggatctt tttttgctct caaggcactc aaaatagaga ccgtatccac
     3601 gacttcttgc aaaacagctc cgaataatgc ggggattagt cccgtacttg caattaacat
     3661 caaaaaaata caaataaaga tgccaatcag tacagactgt ttagctattc tcatcgtatc
     3721 tcttgaaatc actactgctt ttgctacacg caagagatca tctttcaaga tgaccgcatc
     3781 tgcactttca ctagcagctg tagaaccatg agcccccatt gcaataccga tatctgcagc
     3841 agcaagtgct ggagcgtcat tcacgccgtc tcctaccatc gcaacaggtc tttgatcttt
     3901 agggatactt tctaaaatgg tcagtttatc ttgaggcaag ctatgggcat gtacagaatc
     3961 taattggact ttttctgcaa tcgtttgagc aatggcagat ccatccccgg tgatcatcat
     4021 caaatttttc acatcaagtt ttcttagctc cttgattgtc tttgctgctt ctgggcgaat
     4081 ctgatccgaa aataggatcg ttcctgcaaa agatcgatca attgttacat agattgctgt
     4141 attggacgtt tctatttctt cagctgaaac aaaagaggca cgtccaatct ttacttcatg
     4201 attattgact actgcttgga ttccttgacc ggtcacttct ttcagttgac taacaggata
     4261 cagttggctg tttttcacgt tgttcactaa cgaacgagct aagatatggc tagattcctg
     4321 ttctgcacta gctgcataca gaagtagctc ttcttttttt attccattga ctggctgaat
     4381 cgcctctact tccaatatcc ctttggtaag cgttcctgtt ttatcaaaag caatcgtttt
     4441 tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca acgatcccgt ttttacttga
     4501 acggctcatt cctgcaacta aagcaatggg cgcagctaga atcagtggac aaggtgatgc
     4561 cactaccagt acctcagcaa atcgtaaagg gtcttttgag aaccaccaag aaataccacc
     4621 gatcaaataa gcaatcaacg tgaaagggac tgcataacga tcagctaaac ggacaaatcg
     4681 ggcaggcttt tcttttgatt cttcgactag tttaataatc atttgatact ggctgtctgc
     4741 ggcacgtttt tcagcgatca tttggatcga tccgtctcca tttaaagacc ctgacataat
     4801 ctgatctcca ggtttcttgg tttccgggcg ggactcacct gtcaaagaag attcatcgac
     4861 cgaagattct cctttggtca ctcgaccatc tgcgggtaca agttcgccag gcttgactag
     4921 taactgatca tcaatattta tttcatcgat tactatatcc tgcaattttc cttctttgaa
     4981 tcgatgggca attcttgggg tgttatctaa caaagactgc agttctttac ccgctcgttt
     5041 attcgcgtaa tcctctagac tatctccgcc cgtaagcatg atcaatatca tcaaactagc
     5101 ccagtattct ttgacggcta atgttgcaag gattgcggta atcgctaata catctacgcc
     5161 atatcttcct gaacgtaacg tcttgatcat ttccaaaaac atcaagaagg cgagaatccc
     5221 tccagtaaat gcgacaatcg caaatgcaag ggccggctgg tgaaaaacaa attcacttaa
     5281 taaagctatc ccaccaataa gcaaagtaag taaaaatttt ttgaaatgtg tcattgtttt
     5341 ttctcctctt ttatgtaact tagagaggtt agtaataaat ttccagctgc ctctttcatc
     5401 ttaagcatag tccttgacta gcacttttta aaacaatagg ccttaattga aaacaactat
     5461 cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa aaatattcag caaggattac
     5521 tttttcgatt catcagttaa aaagtacctt ttttcgaaaa actatgctat acttttaatt
     5581 cgttatttac ggcggaagga gcaaacatgc gatgaatgag aaacaactag aacaaacgca
     5641 cgtggatgaa acgatcgaac taatccagat cgaacaaaat atgctggata caaaacaaca
     5701 agaattgact caggaaatga atcattcgac aaaagacaca gctaatcaca agattcgagc
     5761 tggttccaat gaagcttttt atgaatctgc tgttgaatat cgccaacatg agcaggaatt
     5821 gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc ctgcaaacgt tgtctgtcat
     5881 gaaagatagt ccttattttg cccgtatcga ctttaaagaa gaccgtgaaa cagaaacatt
     5941 atatctaggt atcgcttctt taagagatct ggcagaagaa acaatcgtta ttgactggcg
     6001 tgcaccaatt gccaatcttt attatgaagg tgagattggt cccgcatttt atgaaactga
     6061 aatagataga atagatgtcg atctgcagtt gaaacgacaa tttaaaatcc aagaaggtcg
     6121 tattttatct atggtagata cgtcggaagt aatcaatgat gactttttgc tagagatatt
     6181 ggatgaatca tcaagcaacc atatgaaaaa catcgtctct acgatacaaa aagcacaaaa
     6241 tgcaatcatt cgggacacaa caagtaaagt aatgctgatt gaaggaatcg ccggaagtgg
     6301 aaagacatcc gctttgcttc aacgtattgc ttttctgttg tatcataatc gcaagtggct
     6361 cgaggctgaa aacgttcttc tattttcacc caaccatttg ttttctgatt acatctcgac
     6421 tgtattgcct tcattaggtg aaagcggcgt gcctacacag acattcaaaa attatttggt
     6481 gcagctgctg ccagagtttt ctttactaga tgaacagcaa caagaaagtg gttttttgct
     6541 aggggaaaaa gatccgatac aagtgatgaa aagcggattg accttagtcg atcagattga
     6601 tcgctatatc caaggcatca caaactacgg tcccttgttc cgtgatatga aaataaatgg
     6661 ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat aaagaaacaa atgaacaatt
     6721 gcctttacat catagactct ctctattaca aacgaagctc ctgaaaaaat taggcggtct
     6781 gcaaaaagac gaaacacgtc agcaatgggt caaagatctg gcggaagaac agctccaaga
     6841 actatacgca acagatccaa atcttgaata tacagaacaa aaggagcgtt cattacgtaa
     6901 aaaactggcc aaccaaatcg tacgtaaaag attccgtcga atccatcgag ggatcatgaa
     6961 ttatcaattt gtcaatataa cgaaacaata tctccacttt ctacaaactg tgccactttc
     7021 tcttctaaca cgacataaaa tcagcgagac cagatggcag gaacatttga caactgttcg
     7081 tacacacttg cgtcaaagag aattgcgtca agaggatgct gtactattct ttttactgat
     7141 gcgccgaaca tatccagtga cggttacaca caaagctcgc tatatcttta ttgatgaaat
     7201 gcaggatttc gcaccatcac aagtggcatt gctgcaagcc ttgttcccaa aagcaaattt
     7261 gactttctgt ggagatctaa atcaaaatgt ctttggaaat gaaacgatcg ttggatcatt
     7321 ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa ttaacgacaa gctatcgctc
     7381 cacaaaagaa atcactgatt ttgctaatca ttttattgtg aaagaacatc aagtagaaac
     7441 aacagcaaga aaaggagacc ttcctttgct catccaagga acaacagatg atatcaaatt
     7501 gaattggtta gcagatacca ttcgaacgac tcagggaaaa gcccggtatt ggcgtacagc
     7561 aatcatagct aaaacacaag aagaatgcga agctctttat gctcaactgc cagacgagtt
     7621 gaaacaacaa gtccagctga ttttaaatga gagtgacttt atgaaacgtt ccattatttt
     7681 acttccagcc tttttagcaa aaggtctgga gtttgaccga gttttcttgt gggatgtaga
     7741 tgcagaacat ttttcttctc agcaagacaa attgatcctt tacaccatgt gtacacgtgc
     7801 gatgcatgaa ttgacccttt ttacctccaa taatgaaagt cctttgttaa acaatatgga
     7861 tatttcatct tatgagaaaa aagtactgta agtaaaaaga caactgctat cattaatgtt
     7921 agcagttgtc tttttattta caataataat tgcagaaaaa gtaattcaat catttaaggt
     7981 catttattct atatataaat ccaaatctgt ttcaggatat tttaatttga caagcgaaat
     8041 aatcattttc agttcttcca tgttttctgg atattctggg tcaaatacct tgctatgacg
     8101 gccaatcccg ccattgtcga tcaatcggta aattcgattc cattcaatga attggtcaat
     8161 tgattggaaa ttgctgattg gatagccgcc acaatccaaa atctctgata cacgaaatgc
     8221 taggtatgca ctatcgatca cattattttt tttcgtgtct atcttctcat ttctttcgat
     8281 aagaattttt tcaggacgat gcttcaagct ccactctaat agtagaaaga aagcctccat
     8341 ccgatactct tcttcaaata accgaaaggc tgaatcagaa cgacgaaaaa gcagcggaat
     8401 cagataattt tgtctataaa tcagcattgg tgcgcggtcc accatacact ctaaataacg
     8461 gttcagatac cctgtaccga catagtttcc taatcgatag ccttcaacgc cgaatcttgc
     8521 gtatttttgc gctaattttg aacaatcagt tacgttcaca gctaactctt ctactttgcg
     8581 gtaaccgctt aagatacgtt caaaaggttc taaagtgatt tttgcttcca tattcttccc
     8641 cgtccatctt ccacatactt catatcattt cacatgatac ttatgctctc atcataccac
     8701 aggttgaatt agaaaagaaa ataaatcaca caaaaaagca agacaatctg ttagtgagat
     8761 tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa actggttgct tagtcttcta
     8821 atgccacatt atggaacaca tgctgtacat cttctagatc ttccagtgca tcaatcattt
     8881 tttcaaatgt ttcttgatct tctggagata gtgttacctc gttttgaggg atcatttgga
     8941 tctcagcgat tgaaaaatca gtgattcctt tttcttttaa tgcttcttgt atactatgga
     9001 aatccgctac ttcaccgtaa acgatgattt gtccttcttc ttccattaca tcgcgaacat
     9061 cgatgtcttt ttccattagg tattcaagga tctcgtcagc gtcttctcct gcaaaagcaa
     9121 agattgctgt attgtcaaac atgtaagaaa ccgctccgga aacacccatg ttcccgccat
     9181 ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt cacattgttc gtcaatgtat
     9241 caacaatcac cattgagccg cttggtccaa atccttcata gcgcaattca gagtatgttt
     9301 catctcctgc accttttgct ttttcgattg ctcggtcaat aatatgtttt ggtacattat
     9361 aagttttagc gcgatcaatg acgaaacgta gtttttggtt tgcatgcgga tcaggatcac
     9421 ctgattttgc tgccgcgtag atttcaatcc caaattttgc ataaatccgg ctattattag
     9481 catctttagc agttttcttt gctacgatat ttgcccattt acgtcccata ttttcacttc
     9541 gctttctgat ttgatagttt cataacgcaa aaacgtcctg ttccattata cttgtgaatc
     9601 acgattctgt gaagcttttt ttctttcttc cttcattctt ctcattttta gtcaattatc
     9661 tatactcgga ttgggatttg cccgaaattc tgttccggaa tattgcctcc caattgatag
     9721 atttgatcac ttctaactaa cagttccgct tcttgtgcct ctttttttcc gattcgggaa
     9781 taaatagaaa tctctttttt ttcttttaag aatcttcttc caacatcggt aaatccaagt
     9841 agtcgaagat aatttttatt ttgttggaca ggaatctctt gctttttgat attcagcaag
     9901 acataacaag ataatcgttg gatccgtgtc catgtatatc tttttgtttt tacctttgac
     9961 acaaaatcct caaaactatc agcagttgca gctgctgttt tcatgcgcga ttctattcct
    10021 tcggtcatct gatagatatc tgtcagttcc agcaaagtag aagataacaa ccgataacgc
    10081 aaatacggcc aataagattc ccaagtctgc agcgaataag ttgaaagatc ttcttctgtc
    10141 tgaggcggca caagtccagc tattcttttt ccttcttcta acgcatggcg aatggctgtt
    10201 gcactggcta tcctctgatc tgtgatgatt ttttcatgat agcctgcact tttacgtcgg
    10261 atcggatgta attccatagg gtggacatac ttggcatttt cctttgcata acttaatccc
    10321 aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg gatacagact gcgaaaaacc
    10381 tcagtcattt tttgcggata actatacgtt tgatcagaca gttcttgaaa tgcctcttct
    10441 atcctatctt gattctcttg gacaaagcgt ccgaaactgc tgtaatcaaa cgaagtctct
    10501 tcttctgttc caaaacacaa actgtcacag tccagtgttt gcaagatttt tactgcacct
    10561 ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg gaagctccac tactaaatcc
    10621 acaccatttt gtaaagcttc attcgcgcga tgccatttat cgatgatagc cggttcgcct
    10681 cgttgcaaaa aattcccgct cattacagca atgacgacct ctgctcctgt tgtttttcgt
    10741 gccatttcca catgatagcg atgtccattg tgaaaaggat tgtactctac gataatcccg
    10801 cagcttttca tgcgcatcct ccttcatagt tatcgataaa aaagaaatag agacaagtaa
    10861 aaaaaggcaa aaatcggaaa aaatacccga actatataaa ctattctcgc aaacacaata
    10921 cttctgcgac gaaaagctgc actgcaagag caatgcctat atcgttcgga tatataattt
    10981 atgttgaaga tttattcttt tcttgttaac tcgagcaggc aaaaccgcta gtctttgtat
    11041 gctacaaaga accagcgctt tgatttctct gatgctgctt cgtctgtaaa atcagaaaat
    11101 gcttcgactt tcgaaaagcc gctgttttcc agcattctta aataactttc caatggatag
    11161 gtacgctctt gatgtagttc atcttcacga ataaatcgac cagacccttc ttgttcttca
    11221 acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc ttcctggata actatcccac
    11281 aaaaaagcaa aattatcccc ttgataatga tagctatatt ctggaaaaat ttcatctact
    11341 tgataggtgg agtgtacgtc aaataaaaat cggccatttt cttctaacgc ttgatagact
    11401 tcatcaaaca cttgctgtac ctcttgtcta tttttcatat agcacagcga atcagaaaaa
    11461 caggtaattg cctgatattg tccgatttct gataaatcga gcatattgcc ttccacaaat
    11521 tggatcgatg cgtcttcttc atatgctcgc tggctggcaa tcatcaacat ctcttctgaa
    11581 aggtctaacc ctgtcacttt atatccatct tttgcaaagg caactgctaa tgctccagta
    11641 ccgcatgcca gttccaaaat attttttgtg tcttcttgta aatgacgttt cgaaaaagct
    11701 agccattgct gataaagtgt ttcgtccatc acttcatcgt aaatgaaagc aaatgtttca
    11761 taagccatat gttaatccaa ccattcacta agatcgacta gcggagcatc tgaccatagt
    11821 ttttccagat tatagaaact tcgttctggt gcatggaaaa catgtacgat cacatctcca
    11881 agatcgatca atacccattt tccgccttct tttccttcaa tacgttttac ttcatatttg
    11941 ttttcttctt ctttatcaat gatttcttct gtaattgcgt tgatttgacg ttcgctattt
    12001 gctgaacaga tcatgaaata atctgccaac aaggaaacat ttcgaatgtc taaagcgata
    12061 atgtcttctg cccgttttga atctgctgct tcaaccgcaa tttttaattg ttctttacta
    12121 tctatgatga tttcctccta atatgagcag cttatttttt tgctacccag taattatatg
    12181 tttcgattgt ttttggataa atctgttgtt cttgcgcaag caaatgggcc aatgtatgtt
    12241 ttgtttcaaa tgctacagct tcgtctagat cagcccatgc aatggctcgt gcatcttgta
    12301 ccccaggaaa atctcgacct ggttcaatat agtctgctac ataaatgatt ttgtcaagga
    12361 gactcatctt agcagcccca gtcgtatgca cccggattgc atgaagaatt tctacatcgg
    12421 tgattcctaa ttcccgctct acaaacgaag cgccaacaag accatgccag attgcattat
    12481 tataattcaa taactctgga tcaaaaccgt ctcttacgat taccattttg aactcctcat
    12541 ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat actagctttt tcaggagaag
    12601 caccgtattt tttggcaaga gccacggctg tttcttctac tccaagcaca tgcttgaagc
    12661 gttgttcgct cattctcatc tgtatttttt gcatcaggtt ttctctagaa taagcagtat
    12721 attcattact atagtccata ttcatacaac ccttcatttt gaatataatc aattacttta
    12781 tctggaacaa ggtagcggat agaacagcct tctttgatct tttgacgtat tttcgtggaa
    12841 ctgatgtcga tctcaggtac atctacccaa attacaggat atggagtatc cgttgtgtat
    12901 cctggtcgac gtatacccac aaagttgacc atagaagtca gctcatcgat tttataccat
    12961 tttggcaaat attctaccat atccccaccg ataataaagt aataatctgt atccggattg
    13021 ttttgcgtca attccttcat agtatcatag gtgtaacttt tcccaccacg agctaattca
    13081 atcgtttcaa tctgcaaaaa aggattgtct tccacagcta attccaacat gttgagtcga
    13141 tgttttgcat caatcgtctg tttctcatca acatgaggtg gctgataaga aggcatcaaa
    13201 aatacgcggt caagacctaa attccggcca gcttgttcag ccatcactaa atgtgcaagg
    13261 tgaacgggat tgaatgttcc gcccaagatc cctacttgct ttttcttctc taagaattga
    13321 ggcatttctt caggaaataa ttgtgaacga acaaatgtct tagcttgcgt attcatcatg
    13381 gtcactcctt atattgcttt tacttctttt gatagccttt gatacttttc ttttgtggaa
    13441 ggtttgtata atacaataac acgtccaatg atttggacga tttcgcaatc tatttctttt
    13501 tctaatactt gagctgcttc ttctgcaatc tcatctgtat tttgaagtaa cgatactttg
    13561 atcagttctc ttttttctaa agcttcttca atttgaacga tcattgctga attgatccca
    13621 ccttttccga tttgaaaaat cggctgaaga tgatgcgctt tgctacgtaa aaaacgcttt
    13681 tgttttcctc ttaaactcat aattttcctt cttccttaaa tcaatgcttt gcgtttcaat
    13741 acatctacac cctttggcgc ccagcccgca attactactg gcgctgttac agtgatccag
    13801 cctagaccgg caaaaacgat gtctgttttt tctttcacag aaaactcaaa acgaacaagc
    13861 tccggaaatg cctcgacttc atcaggacga ggaggctgta ataatccacc tacatgtttt
    13921 tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa tatttacatc atttgacaca
    13981 taagcaataa atgaagcacg attgccgctg ataaaatcaa atcttgcaag tccgcccaag
    14041 aaaagtgttt gcccttcatt cagctgataa actttaggtt tgatttcttt taacggtgca
    14101 atgatccgca aatctttttt acctaagtag tgcgccattt gatggcgatg gataattccc
    14161 ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct cgattttatc caaggtcgtt
    14221 cctggaaact gcgaagttgt gattacatct tgtactcccg ctgtattttt gatgatttga
    14281 ttgataagcg tcgattttcc gacatttgtt actccgacga tatagacatc ccgtccatcg
    14341 cggtattctt cgattttttc taatagttcc gtcatctcat gagatttctt agcactagtt
    14401 aataatacat ctactggacg aattcctgct tcgtaagcac gttccttcat ccattgtgtt
    14461 aatttagttt ttttcaatga tttaggtaat atatccactt tgttcccaac taaaagtact
    14521 ggattgtcac cgacaaaacg gtgtagtcct gggattagtg aaccattgaa gtcaaaaata
    14581 tccaccacat tgacgatcaa tgcatctttc tgccctaatt cattcagtaa gcgcaaaaag
    14641 tcgtcatccg tcaatgagac atcttggatc tcattataat ggcgtaatct gaaacaacgc
    14701 tgacaataga cttccccgga agctaaacct ttttccaaag ccgtttgcgg cgtgtatcct
    14761 aattcaccgg gtttgtttgt ttgtataacg gcaccacagc cgatacattg taattcttct
    14821 gtcattcgat tccacctcgc cagcccatat cagggtgttt tgctaataga taggccataa
    14881 ttcttttttc ccaaaaacga ttaaattgtg tcttccaact atctgttgcg ataatgggtt
    14941 ttacaaggat gctacgtatc cctgcacgat tggctccacg tatatcagtc ataatctgat
    15001 cgcctaccat tactatctca tcatctgcta aatccaattg tttctttgct atgttgatcc
    15061 ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag atcaaatttt tcaatcgctc
    15121 gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac agggattcct gcatttttca
    15181 tttctaaaat ccaagtaagc aactcttctg ttccatctgg attattccat gcaatcaacg
    15241 tattgtctaa atcggtcaat actgctttga tgcctagatt ctttaattgg gcaggtgtaa
    15301 tctgatagat ggcttctacc atccatgttg gtttgtaatt tgaaaacatg ctgtctcctc
    15361 atctgcaaaa aatgaaggag cacataaagt actcctcaac cccgtattat actgcatatc
    15421 agcaaaaaaa actacctcta tactcgttcg tctttcagaa aaagcatttc tttacaaaat
    15481 gaccaaaaat aaagattgat cacagaaaaa tttagaaaca aaaaacaact aagcgagcaa
    15541 gaccattctt tactctttgc ttagttgtta aacacctaat attggcggaa cctcttgtat
    15601 cgtttttcca gtaattcatg ctggtcgaat cgaacaagtt ccgtcaatct ttcaatcaac
    15661 gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt gaacgtttca
    15721 ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat tttcatcaac
    15781 tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa tccttctgga
    15841 gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa acctagagca
    15901 agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac ctttaaatca
    15961 gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca
    16021 ccgcaatatg cacctgctgt attgatcaat gtaatcaccg gtctattgaa cttttccgct
    16081 tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc aaaatttctg
    16141 cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac cggtttgcca
    16201 tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaacaaacg atcaccatgg
    16261 cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca tcgttcttta
    16321 gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc atcctttctg
    16381 tgtatgcaat tcaagaagcg tatggatttt ctgcttcaat tccatgcggg gaacaatctg
    16441 atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg gaagttcctg
    16501 cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc
    16561 tagaatgata tcgccttcca ttgcaaaact ggcagttact cctccggtcg tcgggtccgt
    16621 taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag cagagatttt
    16681 tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg aagctgtgaa
    16741 caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga tcttttctcc
    16801 gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa taatgaaggg
    16861 aatcccttga atggttgctt taccagtgag gactgcttca tgaagtcctg ttttatcttg
    16921 cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg ttacaagatc
    16981 cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct gccatgcacc
    17041 gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt ctttcgtata
    17101 aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag atggcgagtt
    17161 agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct taaacagagc
    17221 catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa
    17281 aaatgctgtg ctgtaatcac ctgctatgac tgcagggtga ctaatcaaat ccatctgaaa
    17341 ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct gcatcttcat
    17401 taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg aatcataata
    17461 tggcgggatc gtatatcccg ggtaaactgc actgtctacc cgcagtccca tcccacctgc
    17521 tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag caggattttc
    17581 tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt gacacacatt
    17641 caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc ctgtgaccat
    17701 ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat aaaaatcgcc
    17761 ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg ctttagctgc
    17821 tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta cagaaggaga
    17881 ttcttccagt actttttgat tattacgttg caaagaacaa tctcgttctc ctaaatgaat
    17941 gacattgcca aaatgatcac ctaaaatctg aacttctata tggcgagcag gataaatgat
    18001 tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct gagctgaaaa
    18061 aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc cgccaccacc
    18121 tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca atgcttcttc
    18181 tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt cttgcatcaa
    18241 ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag gaccaataaa
    18301 tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata aaaaaccaaa
    18361 tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt cctgaacatt
    18421 cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt
    18481 atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc ctagttcgcg
    18541 gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata ctttagaaaa
    18601 catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt ttaccgtcta
    18661 ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc tcaacttcaa
    18721 ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc aatccgccga
    18781 aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct cctgcttgag
    18841 ccatcgcttc aacaattaat acccctggca taacaggttc ttctggaaaa tgtccttgaa
    18901 aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct tcaaccattt
    18961 cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct tttatttctt
    19021 gtatgttcat tgatttccct ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg
    19081 gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc actccgtcgg
    19141 cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata acttctcctt
    19201 tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgccggt tgcagataaa
    19261 cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat tctttttctt
    19321 tcattgtttc tggagcagtt gttgcttgtt caggtatgac attgactggc tccgaaactg
    19381 gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt ttattcatat
    19441 acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga tcaaattgac
    19501 tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt tcatgcacag
    19561 aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa aacttgcagg
    19621 tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt ctacattgat
    19681 cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc
    19741 ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa
    19801 attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg ctggtgtact
    19861 tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt cttccatcgc
    19921 aagcttcatt gctttagacg caccagaacc atctggagta ggagctgtca tatggtaagc
    19981 gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc gagcttgtgc
    20041 atgttctaaa gattcaagaa ctagaacacc tgctccttca cccatcacaa aaccattccg
    20101 attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga gtgctgttaa
    20161 agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc cagcaagaat
    20221 gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat tcgttccgct
    20281 agcacaagct gttacgatag ctgtacaaat accttttgca cctacgcgca aagcaatatt
    20341 tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc gttttgcacc
    20401 tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga tcccagatcc
    20461 gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc tcatctccat
    20521 tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt tgctatcttt
    20581 tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg taatccctgt
    20641 ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt ttaagctatt
    20701 caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag taattaccac
    20761 tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc catcgacatt
    20821 gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt gtgcaatatc
    20881 ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct tgactttgtc
    20941 tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat tacatgtaat
    21001 gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc cagctttact
    21061 agctgcataa tttgcctgac caatatttcc aatcaaccca gatacactag agagattaat
    21121 gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca tattgaaggt
    21181 tccaatcaaa ttgatatcaa gacatttttt gaaatcttct gcatccattc gcatcacaag
    21241 tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa gcttttcttc
    21301 tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg aaactcctac
    21361 gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct ctccgcgacc
    21421 attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt tgccgattcc
    21481 tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt tcctccttat
    21541 agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac tggtattgtt
    21601 ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc gatgacttgt
    21661 tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg ttgcatgact
    21721 tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt tgtgttgctt
    21781 atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc cagttttttt
    21841 gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg gatcattctt
    21901 ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca
    21961 atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta cttgctagct
    22021 tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac cattttcccg
    22081 cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa cgcaactgct
    22141 tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa actcagtcct
    22201 aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc gatgctgact
    22261 gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc tgtaaagcaa
    22321 agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg ctttacaacg
    22381 gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc actaaataag
    22441 aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt cttcctattc
    22501 aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta attttaatga
    22561 ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga ttccgtcttc
    22621 tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga ataaatccaa
    22681 gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt cagtttcttt
    22741 tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa tatattcctc
    22801 cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg agaatacttc
    22861 cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt cccaaggtga
    22921 tctttccact agaaactgct tcatccaata agatagggat gcttgcagca gatgtattcc
    22981 catatttatc catattggtc agaaactttt cttgcggcat ctttactttt cgggcaattt
    23041 tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt ttgtctttca
    23101 tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata tctctgccag
    23161 tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt ttattttgct
    23221 ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt tctttcagca
    23281 attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca aacaacacag
    23341 cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca atcactagtc
    23401 ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa acaaatcctg
    23461 agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc ccttgtactt
    23521 gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca
    23581 aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa atgcataaat
    23641 cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt gacgaaatcc
    23701 attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc ttttcaggaa
    23761 ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct cacttatagt
    23821 cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt tcctccgtag
    23881 ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg acccgaaata
    23941 gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct tcactgcgga
    24001 tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag gtcccaacag
    24061 taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc ccgattgcct
    24121 cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc agctcagact
    24181 cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact gtttttaaat
    24241 ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt gatgttcaaa
    24301 ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac tatatgagct
    24361 ttttttaa
//
LOCUS       DAZKNO010000025        23829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000025 DAZKNO010000000
VERSION     DAZKNO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.025"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACG8MY_000742"
     CDS             complement(53..787)
                     /locus_tag="ACG8MY_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0429964.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACG8MY_000743"
     CDS             1114..1524
                     /locus_tag="ACG8MY_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0429965.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACG8MY_000744"
     CDS             1517..2218
                     /locus_tag="ACG8MY_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0429966.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACG8MY_000745"
     CDS             2218..3804
                     /locus_tag="ACG8MY_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0429967.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACG8MY_000746"
     CDS             3823..4044
                     /locus_tag="ACG8MY_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0429968.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACG8MY_000747"
     CDS             4041..4802
                     /locus_tag="ACG8MY_000747"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0429969.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACG8MY_000748"
     CDS             5182..5691
                     /locus_tag="ACG8MY_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0429970.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACG8MY_000749"
     CDS             complement(5766..6305)
                     /locus_tag="ACG8MY_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0429971.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8MY_000750"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACG8MY_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0429972.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACG8MY_000751"
     CDS             7318..8169
                     /locus_tag="ACG8MY_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0429973.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACG8MY_000752"
     CDS             8197..8832
                     /locus_tag="ACG8MY_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0429974.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACG8MY_000753"
     CDS             8852..9625
                     /locus_tag="ACG8MY_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0429975.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACG8MY_000754"
     CDS             10033..10830
                     /locus_tag="ACG8MY_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0429976.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACG8MY_000755"
     CDS             10808..11221
                     /locus_tag="ACG8MY_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0429977.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8MY_000756"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACG8MY_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0429978.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8MY_000757"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACG8MY_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0429979.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACG8MY_000758"
     CDS             15998..16558
                     /locus_tag="ACG8MY_000758"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0429980.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8MY_000759"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACG8MY_000759"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0429981.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACG8MY_000760"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACG8MY_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0429982.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8MY_000761"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACG8MY_000761"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0429983.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8MY_000762"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACG8MY_000762"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0429984.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8MY_000763"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACG8MY_000763"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0429985.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACG8MY_000764"
     CDS             21905..22483
                     /locus_tag="ACG8MY_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0429986.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACG8MY_000765"
     CDS             22535..23017
                     /locus_tag="ACG8MY_000765"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0429987.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACG8MY_000766"
     CDS             23134..23508
                     /locus_tag="ACG8MY_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429988.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5115 g         6648 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtttt
//
LOCUS       DAZKNO010000026        23332 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000026 DAZKNO010000000
VERSION     DAZKNO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.026"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1690
                     /locus_tag="ACG8MY_000767"
     CDS             29..1690
                     /locus_tag="ACG8MY_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0429989.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1760..2107)
                     /locus_tag="ACG8MY_000768"
     CDS             complement(1760..2107)
                     /locus_tag="ACG8MY_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0429990.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2191..3132
                     /gene="opp3b"
                     /locus_tag="ACG8MY_000769"
     CDS             2191..3132
                     /gene="opp3b"
                     /locus_tag="ACG8MY_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0429991.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3133..4176
                     /locus_tag="ACG8MY_000770"
     CDS             3133..4176
                     /locus_tag="ACG8MY_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0429992.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4182..5252
                     /locus_tag="ACG8MY_000771"
     CDS             4182..5252
                     /locus_tag="ACG8MY_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429993.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5242..6195
                     /locus_tag="ACG8MY_000772"
     CDS             5242..6195
                     /locus_tag="ACG8MY_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0429994.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6207..7217
                     /locus_tag="ACG8MY_000773"
     CDS             6207..7217
                     /locus_tag="ACG8MY_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0429995.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7502..8323)
                     /locus_tag="ACG8MY_000774"
     CDS             complement(7502..8323)
                     /locus_tag="ACG8MY_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0429996.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8420..8698)
                     /locus_tag="ACG8MY_000775"
     CDS             complement(8420..8698)
                     /locus_tag="ACG8MY_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429997.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8966..10975
                     /gene="metG"
                     /locus_tag="ACG8MY_000776"
     CDS             8966..10975
                     /gene="metG"
                     /locus_tag="ACG8MY_000776"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0429998.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11454..12041)
                     /locus_tag="ACG8MY_000777"
     CDS             complement(11454..12041)
                     /locus_tag="ACG8MY_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0429999.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12216..13538
                     /locus_tag="ACG8MY_000778"
     CDS             12216..13538
                     /locus_tag="ACG8MY_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0430000.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13693..14469
                     /locus_tag="ACG8MY_000779"
     CDS             13693..14469
                     /locus_tag="ACG8MY_000779"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0430001.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14462..15031
                     /gene="rnmV"
                     /locus_tag="ACG8MY_000780"
     CDS             14462..15031
                     /gene="rnmV"
                     /locus_tag="ACG8MY_000780"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0430002.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15024..15908
                     /gene="rsmA"
                     /locus_tag="ACG8MY_000781"
     CDS             15024..15908
                     /gene="rsmA"
                     /locus_tag="ACG8MY_000781"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0430003.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16359..16988
                     /locus_tag="ACG8MY_000782"
     CDS             16359..16988
                     /locus_tag="ACG8MY_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430004.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17112..18221)
                     /locus_tag="ACG8MY_000783"
     CDS             complement(17112..18221)
                     /locus_tag="ACG8MY_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430005.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18430..18852
                     /gene="mgsA"
                     /locus_tag="ACG8MY_000784"
     CDS             18430..18852
                     /gene="mgsA"
                     /locus_tag="ACG8MY_000784"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0430006.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19039..19137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19175..19711
                     /locus_tag="ACG8MY_000785"
     CDS             19175..19711
                     /locus_tag="ACG8MY_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0430007.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19751..21514)
                     /locus_tag="ACG8MY_000786"
     CDS             complement(19751..21514)
                     /locus_tag="ACG8MY_000786"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430008.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21517..23232)
                     /locus_tag="ACG8MY_000787"
     CDS             complement(21517..23232)
                     /locus_tag="ACG8MY_000787"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430009.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7502 a         4285 c         4759 g         6786 t
ORIGIN      
        1 tgaaaattcc aataaagggg tacgtaaaat gaaaaagaaa tttacatttg gtttggttgc
       61 ggtatgcggc cttgtacttg ctggatgtta tggcggcagt tcagccacaa gcagtagttc
      121 agcaaaaagc ggttcagcag atggaggagg cgtatttaac ttagtcgtgc ctcaagaaat
      181 gcctacagct gacttatcag ttgcaacaga tacaatcagt tttacagcat tgaataacgt
      241 ttatgaagga atttaccgtc ttgatgaaga cagcaaacca cagccggcag gagctagcga
      301 gttggcagaa gtcagtgaag acggattaac ttacaaaatc aaattacgag aagacgcaaa
      361 atggtcaaat ggtgatcctg ttacagcagc ggattacgtt tatggctggc aacgaacagt
      421 agacccagct acagcttctg aatatgctta cctgtatgcg ccagttgaaa atgcagaaga
      481 cattacagct ggaaagaaag ataaatcaga actagggatc aaagcagttg gtgactatga
      541 attagaaatc aaattgacga aacaaacacc atatttccaa tatctgcttg ctttcccatc
      601 atttttccca caaaaacaat ccgtagtgga agaaaatggg gatgcctatg catctgcaag
      661 tgataaagct gtttataatg gaccattcac attagaagat tttgatggac caggaacaga
      721 tacagaatgg acttataaga aaaatgatca ttactgggac aaagatacag taaaactttc
      781 tgaaatcaaa gtcagtgtag taaaagaatc ttctactgca ttgaacttat tcaaagatgg
      841 ccaagcagat gacgtgatct tgtctggtga gttagcacag caaaacgcaa acgatccagc
      901 ttatacttct gttaaagaag cgcggacaag ctatatcgaa ttcaatcaac gtgaaaagaa
      961 ttcaccattc aacaatgtga acctaagaaa agctatttct tattctatcg atcgtgaagc
     1021 tttagtaaaa caagtactag gtgacggatc agttgtctct acaggattga tcccatcaga
     1081 tatggtaaaa agtccaacaa cgaacgaaga ctttgcaaaa gaagctggaa aattagtttc
     1141 ttatgacaaa gataaagcaa aagaatattg ggaaaaagcg aaaaaagaat tgggcgttga
     1201 ttctttagaa tttgatttga tggcttctga tgatgattca tcgaaaaaag taatcgaata
     1261 tgttcaaaac tcaatccaag aaaacttgga tggcgtaaca gtcaaaccaa cacctgttcc
     1321 attctctgta cgtcttgacc gctcaacttc gggtgatttt gacgcagtac taggcggatg
     1381 ggctgctgac tatgcagacc caagcagctt tactgatttg tttgtcacag gtaattcata
     1441 caaccgtggt cgctggagca atgcagaata tgacgaagca gttaaagcag cttcatcaac
     1501 agacgctggc gatgaacaag cccgttggga tgatttccaa aaagcaatca agatcatctc
     1561 tgatgatatg ggtgtcgttc cggtgtacca aaaagcagaa ggtcacctag tgaatgaaaa
     1621 aatcaaaggt atcgttcacc atgcggcagg cgcttcatgg gattacaaat ggacatacgt
     1681 tgaagaataa tcgttaatac cgtgagcatg agaaaaaacg aggctgcggc aaatgtcgca
     1741 acctcgttct catattcact tatcctaaca aagaaaaaag catgaaaata gcagaaagag
     1801 caattagtat tccggcaatg atcagccaaa agctgtacat accgtagctg gcagacgaga
     1861 gcgaggaaaa ttctggtttt ttctttcggt tccttgttcg tgcaagatgg accgatcgct
     1921 gaaaatgatc gaagaaaccc gaagaaaaga cccataaaaa accgccgata atcagaaacg
     1981 gcaatgacca taagaataga tcattagata attgaatgag tgtaaaggaa tggttgatga
     2041 gattttttat taaaataacc agaatcagta gaaaagctac tgcgtagggg atagattttt
     2101 ttttcatttg attgctcctt tatttttggt tcattatcta ataatgtagc gaaacatctg
     2161 tcagaagtca aaagctaggg ggaaacatta gtgaatagtt atatcaaata tgtattaaaa
     2221 cgtgtctttt tcatgatcat cacgctttgg ttaattgcaa caatcacctt tttcttgatg
     2281 caattgctgc cgggcacgcc atatacgaat caggaacgtt tgagtcctga aacaatcgag
     2341 atgctaaata aacaagtcgg tttggataaa ccagttatcg tacaatatgg gatttatctt
     2401 tctaatttgc ttcaaggaga ttttggaatt tccttccaat ttaaaaacca gccagtcgca
     2461 catttgttag ctggaagaat cggaccatcc ttgcagttag ggttacaggc aattatcttt
     2521 ggaacagtcc tagggacgat tttaggaacg atctctgcca tgaagcaaaa tacatgggca
     2581 gatacatctt ctacactcgt agcgatatta ggtcgttcca ttccaaactt tgtatttgct
     2641 gttttattac aatatatctt tgcaatcaaa ttacaagttt tgccaatcgc aaaatgggat
     2701 ggctttgtgt acacaatctt accaacaatt gcgttggcca tgtcgccgct tgctgactct
     2761 gctcggttta tccgtacgga aatggtggaa gtactgcata gtgattatgt ggaattagca
     2821 aaagcaaaag gattgagccg ttgggaaatc gcctttaaac atggtcttcg aaatagtttg
     2881 attccattga tgactttatt aggaccgtta gctgtagcat tgatgaccgg atcactggtg
     2941 gtagaaaata tctttgctat tcctggtatc ggggaacaat tcgttaagtc aatcacaaca
     3001 aatgattatc ctacgatcat ggctgtaacg atcctttact catttatgct gatttttgtt
     3061 attttgatcg tggatctatt gtacggtcta gttgatccaa gaattcgtgt ttcagaaggg
     3121 agccgaggat aaatggaaat gataccaaag cgttatgaaa cagttgcaga tatccctgcg
     3181 gatgaatttc ttccattaca aaaaaatacc gaagaagaac gtgaacagat tactgcacct
     3241 tctttgaatt ttatccaaga ttcatggcgc cgtttaaaga aaaacaaagc tgccgttatt
     3301 tcaatggttt tactattggt catcattttg atctctgtga tcacaatctt tgtttctcct
     3361 catgatccaa ctgcccaaaa cgtggcttat atcaatttgc ctcctcgtat tccgggaatc
     3421 aatatcgatg gattgaatgg aaaagcaatg gttggtggtg aacttgttga taaatatgct
     3481 caagcaaacg tcccttcaaa cgtaaatttc tttttaggaa cagacggttt gggacgtgat
     3541 gtattaagcc gcttatttat gggaatgaga atctcgttgc tgatcgcatt tattgctgca
     3601 ttactagatg taacgattgg tgtagcttac gggttggttt ctggtttgtt aggcggacgt
     3661 gtcgataacg tgatgcaacg ttttttggaa atcttatcag gaattccaaa cctcgttgtc
     3721 atgattttga tgttggtcgt ttttgaacca ggaatttttt caatcgtggc tgctatggcc
     3781 atcacaaact ggataccaat ggcacgtatc gttcgtgcac aaacaatgaa attaaaagat
     3841 caagaatatg ttttagctgg attgacatta ggtgaatcaa gaatgaaaat tgcattcaaa
     3901 catattttgc cgaatatctc tagtgtcatt attattcaaa tgatgtttag tattccatct
     3961 gctattttct tcgaagcctt tttaagcttt atcggtttag gtcttacgcc tccgtcagct
     4021 tcattaggaa cgatgttgag cgaagggtac aagacattcc tgtatctgcc ttatctatta
     4081 tggattccgg cagtaacttt atcggttatc atgattggct ttaacttact tgctgatgga
     4141 ttgcgtgatg cctttgatcc taaaatgaaa gagtgaagac gatgacgaaa attttagaag
     4201 tgaaagactt agaaatctct tttgatacgt atgctggaaa ggtacgagcg attcgcggtg
     4261 tcaattttca tttgaataaa ggagaaacat tggcgatcgt tggggaatct ggaagtggaa
     4321 aatcagtgac gaccagaagc atcatgcgct tgctgtcaag caacgccaat atagatgctg
     4381 gtcaaatcct ctttaaaggt aacgatatcg ttggaaaatc agaaagagag atgcaaaaaa
     4441 tccgtggtaa agaaatagcg atgattttcc aagatccaat gacctcttta gatccgacga
     4501 tgcccattgg gaagcaagtc gctgagtccc ttagaaagca taataaagtt tcaaaaaaag
     4561 agggacaaaa agctgcatta gaattgctta aattagttgg aatacccgaa gctgagaaac
     4621 gtatcaacag ttatccgcat cagttctcag gaggtcagcg tcaacggatc gtgatcgcga
     4681 ttgcactggt atgttatccg gaaatcctga ttgctgatga acctacaaca gcgcttgatg
     4741 tgactattca agcacagatt ttggagctgc tgaaagaaat tcagacaaaa atcgatacat
     4801 caattatctt tatcactcat gaccttggtg tagtagcgaa tgtcgctgat cgtgtcgctg
     4861 tcatgtatgg aggaagaatc gttgaagttg gaacatcgga agaaattttc tacaacccgc
     4921 aacatcccta tacttgggga ctgcttggtt caatgccgac attagatagt gcgaacgatc
     4981 gattgtatgc tattcctggt tctccaccgg atttattgaa cccgccaaaa ggagacgcat
     5041 tctatccaag aaatgaattc gcgatgaaaa tcgatgcaga acaagaacca ccattttttg
     5101 aactatcaaa aacacacaag gcagcaactt ggctacttgc tccgcaggca cccaaagtga
     5161 caccgccaga agaaatccaa cgtcgctggg ctatctttaa agagaaacaa aaacatactt
     5221 acggtcaagg aggaattgct gatgccaaat aaactacttg aagttaaagg attgaaacag
     5281 tatttcaatg taggaaaaaa gaatgaagta catgctgtaa atgacatcag ctttcacatt
     5341 tatgaaggag aaacattcgg tttagttgga gaatctggaa gcggaaaatc cactactggc
     5401 cgtacgatca ttcgcttgaa tgaacctact gatggtgaga tattattcga cggtcaagat
     5461 ataacgaaga tcaaagataa aaaaggtttg acgaaattcc ggcatgatgt tcagatgatc
     5521 tttcaagatc catacgcttc gttgaatcct cgtatgaaag tcagagatat cattgccgaa
     5581 ggaattgatg ttaatggact agctaaatca tctgaagagc gtgcaaaaaa agtcgatgat
     5641 ctattgagaa ctgttggatt gaatccaagc cacggtactc gttatcctca tgaattttca
     5701 ggtgggcaac gccagcgtat tggtattgca cgagcattgg cagtaaaacc gcgttttatc
     5761 atttgtgatg aaccaatttc tgcactagat gtgtcgatcc aagcccaagt ggtcaatttg
     5821 ttgcaagatc tccaaaaaga acatcaattg acctatctat ttattgcaca tgatttgtcg
     5881 atggttaaac atattagtga ccgaatcggt gtaatgcata acggcttgtt attagagatg
     5941 ggaacaagtg atgaaatcta taatcacggt gttcatcctt atacagaaag tttgttatct
     6001 gctattcctc taccagatcc agaccatgag agaaaacgca gaagaatcaa atatcaacct
     6061 gaaccagatg acggacaagt acgtcaactt cgtgaaatcg caccagaaca ttttgtctat
     6121 gcaactgagc aggaagtccc atattacgca aaaaaactaa aacgacaaaa ggaatctctt
     6181 ctggttgcta actaggaggg acatagatgc taaaagcttt taaattccgt atgtatccga
     6241 cagaagaaca aaaacagcaa ttgattcgta cttttggatg tgctcgattt acctataacc
     6301 atttgctgaa aaagcggcag aaaagttggc aacaaacagg agtagccaat ttttcgctca
     6361 cacccgctac attaaaaaaa gaatacccat tcttaaaaga agtagacagt cttgctttag
     6421 caaatgcaca gcttaatttg gatcgggctt ttcgcaatta tttcaaggga cgtgccagct
     6481 tcccaaagct taaaacgaaa aaaagtatgt ggcaatctta tacgaccaac aaccaaaccc
     6541 gtacgatcta tctggaaaat ggctacttaa agttaccaaa acaaaaagaa ctaatcaaga
     6601 taaatcagca tcggccagtt gaaggatcga ttcgctccgc tacgatttct gctcgatata
     6661 atgaagaatt ttatgtcgca ctactttgtg atgtttcgcc agtaaaaaaa gaatctttag
     6721 ctaaatggat cgggatcgcc tatcatccga agacattgat ccaaacatct cgacctctag
     6781 aagtaacttt accaaaattt caccagacag aagaaaagct gcaacatgca cagcggaaat
     6841 tgaatgtgaa agtccgctcc gctcaccata gaaagatcag gttagatcag gcaagcaatt
     6901 atcagaaaca aaagagaaaa gtaatggatc tttatctgaa acaaaagaac cagcgagaag
     6961 attatcttga acaattatct gggaaattgg tcaaacaata tgactactta tttgtagaat
     7021 cttttccaaa agaagaggca catgctgatt tttctattca cgactggcat aaattaataa
     7081 caaaattgcg ttataagtcc caatggtata ataaaaaatt tcttttgatc aataccgacg
     7141 gagcagaaga aagcaattcc gtaagaaaga gccaagtagt agaaaaaatg ggcagacatt
     7201 cggttatcaa gggataaaaa atgaatgaaa ctgataggag cgttagggtt agccttgtta
     7261 ataaaaagtc ttaatgactt ttgtttctag gaatttttct tttgaagaaa agctcactaa
     7321 ttatgacaaa ccaataaaac aatagcagaa aaagttcgta cgagcttttt ctgctattgt
     7381 taatttttta ttcagaaagt ttacgaatac atcaaaattc tgaaacaaaa aacgtaactc
     7441 acaaagagtt gtttttgcgt tttttcttga atgattaggt ctgaatcttt tgctcacaac
     7501 cttattcatg gtgccccggc ttgagctgat agatttttga aggacggccg attcctacag
     7561 gacgttcacc gatctcatcg aaatattgga gcatagcttt cttaaaattg gaatgatcga
     7621 tcgttttata atcgatccct aagaatttag caaatacttt tcgagcttcc gtaatcgtga
     7681 aatctttacc taaaacttgt agaacttgcg gctcgtgttc cattttattg actacacggt
     7741 tgaatgcttt caagatgatt tgactatggt caaaagccaa agtatctttg ccagaagact
     7801 ctccagtgac taaatcaagc gagatagcca cttctccgct agataaatgg atcttgttac
     7861 tatgacgttc tagtgtgaac cagcgtactt ccttggcatc atctccggcg attagcggtt
     7921 cttctccaat aaaggctaag tagctgactg tgacgaccca gcctcgcgga tctcgattag
     7981 gcgtactgaa tgtatgcagt tgctcaatat tttcttttga aataaccaca ccagtttctt
     8041 cctttgtttc tcttaagacg ctttctccag ttgactcatc cttttggaca aagccaccag
     8101 gcaatgccca tgaatttcga taaggatgcc cttttctttg gatcaacaaa actttcagct
     8161 gatcttcttc tttgttatag cacatcagta cgatatcgac agtcaaagag ggcttgtcat
     8221 attctggacg ttcctgtttt tgataccagt taagaaattc ttcttcactt gctttatgtt
     8281 cataatataa tttttcttct tctttagaag caaaacggtt catctgacca cctccattga
     8341 cagtaaaagt actttttacc ttattgtagc agactctgaa aaaaaaccaa ggatttactt
     8401 gaaaaatcta ggttatcatt taaatatgaa aaataaatgg aagtatgaga ttgaataaaa
     8461 agaaaagcag ataaaacggc aatactttat accgctcttc acgaatggca aagaagccac
     8521 aaataacgat atatacgtca atgataaaac gcaaaatcca agctgctgtc ataaaactgt
     8581 tcaaatgccc atggaggtta aaataaccat acatacgaat ataaaaatag ctgaatatta
     8641 aatgtgctaa tgaaagaaaa gcaatactaa gtgtaaaaaa aagatgtttc ttctgcatga
     8701 ttgttccagt cccttactaa gatagatagt ctttattata ccacgtgtga ggggagaaat
     8761 aaaaagccgt tttttagtag atatgaaaca aaacaaaaac ccgcatttat tcaacctatt
     8821 catcctttta aaggatagat tgaacgtaga tagaagtcgt ttatccagtc tttttcattt
     8881 tgaaaaaaag ctaacagcat gcttttttta tggtatacta gcaaggaaac gaattcgcat
     8941 catgcgataa aggaggaaga gccccgtggc agaaaaggaa actttttata tcactacgcc
     9001 aatctattat ccaagcggca aacttcatat cggcaactcg tatactacga ttgcatgtga
     9061 cgcaattgct cgttataaac ggttgatggg ctttgatgtg ttttatctca ctggagtgga
     9121 tgaacatggt caaaaaatcg aaacaaaagc agaagaacta ggtgtaaaac cgcaagaata
     9181 tgtagataaa atggcggctg atgtcaagaa attatggaaa acgcttgata tcagttatga
     9241 taaattcatt cgcacgacag atgattacca taaagcagct gttcaaaaaa tatttgatcg
     9301 tttattagaa cagggagata tttacttagg tgaatatgaa ggctggtact ctgtttctga
     9361 cgaagaattc tttacagaaa cacaacttgc agaagtgtac aaagatgacg aaggtaacgt
     9421 gatcggtggt aaagcgccaa gtggacacga agtagaattg gtaaaagaag aatcatactt
     9481 cttccgcatg agcaaatacg cagaccgtct attggaatac tatgaagaac atcccgaatt
     9541 catccagcca gaatctcgta aaaacgaaat gatcaacaac tttatcaaac caggattaga
     9601 ggatcttgca gtttcacgaa caacttttac ttggggagta ccagtcaaga gtgatccgaa
     9661 acatgtggtg tatgtatgga tcgatgcatt atccaattat attactgcac taggatatgg
     9721 ctccgaagat gaaagtctat tcgaaaaata ctggcctgca gatgttcaga tggtcggtaa
     9781 agaaatcgtt cgctttcata cgatctattg gccaatcatg ctgatggccc ttgatttgcc
     9841 tctacctaaa aaagtatttg ggcatggctg gctattgatg aaagacggaa aaatgtctaa
     9901 atcaaaagga aatgtcgttt atcctgaaat gcttgtggaa cgttatggtt tagatgcact
     9961 ccgttattat ttgcttcgtg ctgttccgtt tgggtcagat ggtgtcttta cgccagaaga
    10021 ttttgtttcc cgtgtcaatt atgatctggc aaatgatcta gggaacttat tgaatcgaac
    10081 gattgctatg atcaacaaat attgtgatgg actcgttcct gactatgcct cacttgtcac
    10141 tccatttgat agtgaactgt ctacgacggc tgctaacgtt gtcggacgtt accatgatgc
    10201 aatggaaaaa atggaattca atacagcatt ggctgaaatc tgggtgctga tttctcgtgc
    10261 aaacaaatac attgatgaaa cagaaccatg ggtattagca aaagacgaag aaaagaaagc
    10321 tgaattgaat agtgtcatga tccatttagc tgaatctttg cgtatcgcag cgatattact
    10381 tcagccaatc atgacagaaa cgccagagaa gatcttcaat caactaggat tggactctga
    10441 aacaatgaat cttgaaggat tacactttgg cgaatttcct tcaggaacaa aagtagttgc
    10501 aaaaggtaca ccaatcttcc cacgtttaga tatggaagaa gaagttgctt ttattcaaga
    10561 aaaaatgtct gaaggtactc aaacaaacga agatacagtc aaatgggatc cagaagaaac
    10621 agaacttgtt tcaacgaaag aaaaacaaat caaatttgat gtatttgaaa aagtagaact
    10681 aaaagtagca gaagtcatca actgtcaaaa agtagaaggg gcagataagt tgctgcaatt
    10741 ccgactagat gctggagaca gccaagaccg tcaaatcttg tcaggaatcg ctgaatttta
    10801 tcctgatcca agcgaattga tcgggaaaaa agtcgtcatt gtagcaaatc tgaaaccacg
    10861 aaagatgcgt ggacaaatca gtcaaggaat gatcctttct gcagaagcac cagatggctc
    10921 tttacaagta atcgaagcac caaaatccat gccaaatggc tcagaaatcg catagaaatt
    10981 caaagggggt atgacactag ttgtgtcata cccccttttt ccgaaatttg agaagcaatt
    11041 ttcgtaaatt ccttcttatt acttgaagcc aatcatataa tacagaaaca gagaactatc
    11101 caaaataaat cctagaaaaa gaagtacgag cttcgctgat ttttgttaat ttagaataat
    11161 actaatagga atcgtaaaaa cagaaaaaaa gcataatgat aaagggtcgg gcagatttta
    11221 attgattcat ctggtactct tttttattgt ccttacattc ataaagtttg aatgcaatga
    11281 ctttattaca tctatattta tatctaacag tagcgttgat gtgttaatta taagttctat
    11341 cactttatgt tcaattagtt tgtgctctta tatgtgtgat tgaaaatata gataaaaaaa
    11401 caatcatcgg ctttgacttc gatgattgtt ttctttattt tgtaaaaatc ctatcacttg
    11461 attttatggt aaccttttat ttttgtcttc aaacggcttg gtaagagagc agtaagcagt
    11521 gcaaatggga tcgaaaagac ataccagaaa atcgtacgtc cgataccata atgttgatag
    11581 agctttgtca acatcacgtg taacaagatc aaccctaaaa gcaaggagcc agctataaac
    11641 caatgattat cccaatgcat gacttcacta agtgccatca ctccaaaagg atagaaataa
    11701 aaatagatcc cgactaaggt catccgccag ttctttgtaa atgttccgat attcaataaa
    11761 aagaaaatcg ctgaaagcaa aataccgata ggcggaaaaa gcccaaccaa aaaggaatac
    11821 aacatccccc acaagatcat cacaggacct ttgatcaatg caacgattgc gatgaacaaa
    11881 gtcagcaata ttagtttttg tgataacgtt gcaacagtca ttaatccaaa taaaattaat
    11941 gtgaaccaat gccaatggga ctgctgttgt tctttacggg aaaaatcgac ccggttggat
    12001 aaataatctg ctaatttcgg ataagaacgc ttaaaatcca taaactgttc ctccttcgtg
    12061 gaaaaggtag gtttagtata cgaaaaataa aaaagaaata catccgtctt aagagggagt
    12121 ttttcactga aagtggagaa aaaagaatgg taaattaaaa tattctcagt tatactgaaa
    12181 attataccaa atttcaaaga agaaagagga tgggaatgaa aggcaaacta aaacgagaaa
    12241 tcaatctgtt tggcgcatta gctaccgtta tgggaaccgt gatcggtgca ggcgtcttct
    12301 tcaaaactgc tgctgttact gctagtactc agtctacaag tttgacattg ctagcttggc
    12361 ttttgggtgg tttcttaacg atctgtgctg gattgacagt ggctgaatta gctacagcaa
    12421 tcccggaaac tggtggagct gtaaaatata ttgaagctgc gtatggaaaa ttgcccagct
    12481 ttttattagg atgggcgcaa agcttgattt actttcctgc taatattgct gcattatcga
    12541 ttatctttgc gacacaattg acgaacttat tacaattatc taccgattat cttttgttga
    12601 ttgcagtgat caccgctgtt tctgtgacag ggttgaattt gttagggaca aaagtcggtg
    12661 cttctgtaca atcagtgacg ttgattgtta aattaatacc aattgctgtg attgtcatat
    12721 ggggattatt gaccccagga caaggaactg tccagttatt tccaatcgaa gctggaaaag
    12781 atgttacttt tgtcgaagga ttgagcagtg cgttgttagc aacgttgttt gcttatgacg
    12841 gctggttagg tgtcggtgca atggcagggg aaatgaaacg gccggaaaaa gatttgccta
    12901 aagcaattat tcttggtttg agttttgtaa cagtcgttta tctattgatc aattttgttt
    12961 ttttgaaaac tttgccgatt gatcatcttg ccggtaattt gaatgcagct tctgaagcat
    13021 cagacgtcat ttttggagga atcggtggaa agctggtcac tattgggatc ttgatctcgg
    13081 tatacggtgc attgaacggt tatacactaa ctgggatccg cgtgccttat gcgatggctt
    13141 tagaagacga gctacctttc agtaaacagc tgacaaagct ttcaaagaag tttacggttc
    13201 cttatgttcc tgcggttttc cagttagcag tagcttgtat tatgatgagc cttggttctt
    13261 tcgacttttt aacagacatg ttgatttttg tgatgtggct attcagttta ctgattttta
    13321 tcggtgtctt tattttaaga aaaaaagcgc cggaattgcc acgtccatat aaagtaccac
    13381 tttatccaat cgttccgatt attgcgattc tcggtgctat tttcatttta ggtatgacga
    13441 tgatgacgca aacgaaatta gcattgatcg gtataggtgt taccttgata ggtatcccag
    13501 tctattatca aaaaaagaaa aaacgctcag aagagtaaaa tatttcaaca acagacagaa
    13561 aaagtacgaa ctaggtcctc tagatcttcg tgctttttct atttcaataa aacaagttct
    13621 tctattttga tttttgctac ttctgcaagt agtgcttatg tgctaaagta agaatgaaaa
    13681 ggagagataa acatgatatt tgattctcat acccatttaa atgctgaaca gttcaatgaa
    13741 gatattccag aaacgattca gcgagcacaa gaaatggggg taacgaaaat ggcggtcgtg
    13801 ggcttcgata cgccaacgat tgaaaagtct ttgcaattaa gccatgacta tccaaatata
    13861 tacagtatta tcggctggca tccgacagag gctggcagtt atacgaaaga aatcgaaaat
    13921 aaattacaag aacaattgac tttaccaaag gttgtagcat taggggaaat tggcttggat
    13981 tatcattgga tggaagaccc gaaagaagtc caagataaag tatttcgcag acagattgct
    14041 atcgcaaaag aaatgaacct accaatcagt attcatacta gagaagcact tgaagacacg
    14101 tatcaaatcc taaaagaaga agatgttcga gatatcggtg gaatcatgca tagtttcagt
    14161 ggtgattatg aatgggcaaa acgatttctt gacttaggga tgcatatttc atttagcggt
    14221 gtcgtcactt tcaaaaaagc attagacgtc caagaggctg cagcaaatgt accaatcgaa
    14281 cgattacttg tggaaacaga tgccccttat ctggcaccag ttccttttag aggaaagcga
    14341 aatgaaccag gctatactcg ttatacagta gaaaagattg ctgaacttcg gaatcttccg
    14401 acagaagaag tggctgccca aacatggaca aatgcgcacc gattatttgg tctaagcgaa
    14461 catggataag atgaagatcc aagagattat agtcgttgaa ggaaaagacg atacaagaag
    14521 gctgcgggaa gtagttgatg cagatacgat agaaacaata gggtctgcaa tcaatgaaga
    14581 gatcctgatg caaattgaac atgcccaaga gactcgtgga gtgatcgttt ttaccgatcc
    14641 tgatttttca ggagaaaaaa taagaaaaat cattatggaa gctgttcctc aggcaaaaca
    14701 tgcttttttg ccgcgcacac aagctaaacc aaagaaaaag ggaagccttg gtgtcgagca
    14761 tgcaagtgat gctgctattt tagaagcatt gaaaaaggtc gtgacgccag acatttccca
    14821 aacggctgtt tctgaaatca ctagacaaga ccttttagac tatggtttaa tagctggggc
    14881 acaagcaaaa gaaaaacgag aaaaactagg tgacgagttg cgcatcggtt acacgaacgg
    14941 caaacaattg gaaaaacgtt tgaagatgtt tcgtattacg ccgaaagaac tcgcccaagc
    15001 aatgaaaata gtggaggaac aacatgagtg aatatcgtga aattgcgaca ccctctagaa
    15061 caaaagagat tttattgaaa catggttttt catttaaaaa aagtttagga caaaattttc
    15121 tgactgaacc aaatatttta aggaaaatcg tagaaactgc gcaaatagat gaacatacga
    15181 acgtgatcga agttggacca ggaatcggtg cattgacgga acaattagcc aagcatgcaa
    15241 aacaagtcgt agcatttgag atcgatgatc gtttgatccc tgtattggct gatacgatgc
    15301 aaccctatga caatgtgact attgtacatc aagatatttt aaagaccgat ttgtcaacag
    15361 ctgttagaga aacatttcat gaggagcttc cgttaaaagt cgtagcgaat ttaccttact
    15421 atattacgac accgatcatg atgcattttc ttgaatcaga tttggttgtg gatgaactag
    15481 tcgtcatgat gcaaaaagaa gtagctgatc gtatctctgc tgaaccagga acgaaagctt
    15541 atggttcatt atcgattgct gttcaatatt atatggaagc cagtttagca ttcatcgtac
    15601 caaaaacagt atttgtccct cagccaaatg tagattcagc gattttgaaa ctgacaagaa
    15661 gagatatacc ggcagtcgaa gtgacagacg aaaaagcatt tttccgattg acaaaagcgg
    15721 catttcagca aagaagaaaa acgctttgga ataacttgca gcattcgtat ggcaaagatg
    15781 accagacaaa agcatggtta gcaaaaagct tggaaacagc tggaatcgat ccaaaacgtc
    15841 gtggggagac tttgtcatta caagaattcg ccgcattaag caatgcgatg tcagaaaata
    15901 tgcagtaaaa aaagaggttg tgacagaagt tgtgaaaaag ctgtcctgca tcaagtaata
    15961 agaacagcca aacaaaaata gttcctcatg tttttcgttt gtttgggctt attaccgcag
    16021 atgcaagctt ttgaacaccg tttaagaaag aggttgtggg cctgccgttt aactctaagt
    16081 cacaaggaac aatttcaaaa aacagcttct catgtttttg agttagttgg tcttattact
    16141 tgatgcaagc ttttgaccac tattatgccg aacaaggaga tgtgacacaa attttgtcac
    16201 atctcctatt tttgtttctt gatcaaaaaa ttcttatact tgatactcac actcattttg
    16261 tatggattta gaaaagaata tgtgtcttga cagttattag taagtgagtt atactaagtc
    16321 gacatttatg tgtcaagaca tattaggaga gagaaaaaat gagaaaaaat tcagttcgaa
    16381 aactaacgat ttctgcttta ttgattggga tgggggtcat tatcccaatg gtcatgccta
    16441 aaatcatgat tccaccagct tcctttactt tggcgtctca tgtgccatta tttattgcga
    16501 tgttttttac accaggagtt gcagtggctg ttgcgctagg tacgacattt ggtttctttt
    16561 taacaacgcc ggttatcatc gcattgcgag cattgtcaca tcttgtgttt gcgttgattg
    16621 gggcttggta cttgcaaaag catccgagta tcgttttaaa aaatggacag tttacttttg
    16681 ctaattggcg tttccaaggg ttcaattttg tgatcggtgt gattcattct ttagcagaaa
    16741 tgctagtagt cagcatcttt tactttattg gtaatatgcc tgaaacatat tattctcaag
    16801 gatattttta tacagtattc atcttgatgg ggattggtgg gcttatccac agtttagttg
    16861 attataatat tgcttatttt atagcaggaa cactcagcaa atattttgat atccccgtat
    16921 ttacggcagc aaaaaaagaa aatcggaaag agtcgattca agggtacgcg aagaaaacga
    16981 catattagac atgatctctc aaaaaaaaaa ctctcatcta gtaatggata ataaatcctc
    17041 taagatgaga gttttttgtc ctaaaaagag ctgcaccaac atcttctggc acagctctaa
    17101 cagggggcaa tttatggaag tttgatgact tgttctgttt ctttatcaaa gaagtggcct
    17161 ttgctgatgt tgaaggcaag gtcgactatt tcgcctggtt tatggaaatc tcttgcatct
    17221 acttttgaga tgaactcagt agaaccagtt tttgtgtaaa gcatcgtttc agctccaagt
    17281 aattcggaaa cgacaacttc tgatttcact acggcttcag gtgaagcttc aagggctact
    17341 tgttcactgt ggatgtcttc tggacgaata ccgaagataa cttcttttcc ttcataacct
    17401 ttttcaacta atactttgtt ttttccttca ggaatacgaa gcttaagccc atgtccatct
    17461 gtgataacac catttttcaa tgtcacatta aagaagttca tggcaggaga tccaataaag
    17521 ccggcaacga acatattgac gggtgtatca taaacttctt tcggtgtacc gatttgttgg
    17581 ataaatccgt ctttcatgat aacgattcgg tcagccatag tcattgcttc tgtctgatcg
    17641 tgagtaacat agatggtcgt tgtttcaaga cgctggtgaa gtttggcaat ctcagcacgc
    17701 atggctacac gtaatttagc atctaaattt gataatggct catccattaa gaagactttt
    17761 gcatcccgaa cgattgcccg tcccaatgcc acacgttggc gttgtccacc agataaagca
    17821 gctggttttc gtttcaagta ttctgtcaga cctaagatct ctgctgcatt gtccacacgt
    17881 tttttgatgt ctgctttatc atatttacgt aatttcaagc caaaagccat attatcaaaa
    17941 acagtcatat gaggatacaa tgcgtagttt tggaaaacca tggcaatatc acggtcttta
    18001 ggtgccacgt cattcatgac ttttccgccg atatttagtt ctccttcaga gatatcttcc
    18061 agtcccgcga tcattcgcaa agtagttgac tttccacaac cggaaggacc aacaaaaacg
    18121 ataaattcac ggtctttgat tgatagatta aaatcagtta cagaataatt ttctgcattg
    18181 tcatattttt tatatacgtt tttcagagcc atttctacca taatacgttc cacactttct
    18241 tttttagttt tattaactat ttaaagtata aatgaaagcg ttaactaact tcaatgtcat
    18301 gttgaacaag aattttttct tttttttgtc aatgtgctag ttttaagctt taataaatat
    18361 ccataagaaa taaaaactga tacactggag taaataagta tgttaacaat atcaaaagga
    18421 gtgtctctta tgaaaatcgc tttgatcgct catgaccgaa aaaaacctat gatggtgaaa
    18481 ttagcaacag cgtataaaac aatattggaa aaacatgaac tttttgctac cgggacgact
    18541 gggcagagga tcacggaagc aaccggcttg ccgatccatc ggtttaaatc cggaccgtta
    18601 ggcggggatc agcaaattgg agccatgatt tcggaggatc agttagattt ggtgattttt
    18661 cttcgagacc cgctggctgc acagccccat gaaccagatg taacagcact tatccgttta
    18721 tctgatgtct atgagattcc gttagctacg aatatcggaa cagctgaggt gctactaaga
    18781 ggactggaag ccggcttcct agattttcga agaatcgtcc atgaaataga caagcatcca
    18841 ctttcatttt gatggataag cgtatggaaa aataacgttt aatgttcgcc tatatgtagc
    18901 tataaaagct aaacaaactg ataatatgcg atataatacg aacgtattgg ataatataaa
    18961 caataatatt agtttttcgt atttgcagta aatgtgaaaa tatgctatag tttctgtggg
    19021 aaaagatccc aaaaataata cagagtagaa aataaagcgt taagtgctaa gaggatggga
    19081 tgttgcctct tggacgaagg acgtaatgtt cgcggtttta ttttcgcatt cgctgcatga
    19141 tacgatgtag cagctcttaa aggagatgct aaaaatgaag aaacaccggt tagacgcacg
    19201 tatgattgct attatgggct tattgattgc attgatggtc actttgtccc gactggtagc
    19261 aatcgagacc ccatttatta aaatcagtgt cacatttatt ccacaagtga tcatgggcat
    19321 tctttttggt cctttttggt cagggatagg cgcagtttta gctgatttag taggaatggc
    19381 cctcttctcg aaatctgcat tctttattgg atttacattg aatgcattta ttgaaggagc
    19441 aatctacgga ttctttttct atcgaaaaga gatcacttgg aaaaatgcca ttctggctac
    19501 gcttagtgtc acgctgatca tcaacttatt tttgacacca ttatggcttg cgttgatgta
    19561 ccatgtgcca ttgttcagct gggtcgtttg ggcgccccgc ctgttaaaaa cagtaatatg
    19621 gttaccgatt caatcaattg ccatttatta cgtaggacgt agtattcctt acaaaaaaat
    19681 tttaagaagt ttagcgatcc acgctaaata aaaaacaagc agtcttccca tcccagaaga
    19741 ctgcttgttt ttattgtgct gtgaattgtg cttgatagag ccgtcggtaa taaccatctt
    19801 tttgaagcag ctcatcatgg gtgcccattt cgacaatagc tccctgatcc atcactaata
    19861 tctggtccgc atttttgatt gtggacaggc gatgggcaat cacaaaactg gtttttcctt
    19921 ccatcattgc taagaaggct tgttggattt ttttctctgt caatgtatcg actgagctgg
    19981 tagcttcatc taagatcaac atagggggat tactgatcat cgttcgagca atcgtcaaca
    20041 gttggcgttg tccatctgat atctttaacc cttgaccgcc aatcttagta tctaattttt
    20101 ccggcaaacg ttctacaaat tcatacatat aagaggcttt tagggcatca ttgatttctg
    20161 cgtcacttgc ttccggattc ccaaaacgaa gattttctcg caatgtactg tcaaatagcc
    20221 aagtatcttg taacaccatt ccaaaacttt tacgtaatgt atcacgttgg atagagcgga
    20281 tgtcatggtc atcgactgta attgttcctt ttgttgtgtc ataaaaacgc atcagcaaat
    20341 taacaagtgt ggattttccg gcccctgttt ttcctactat agcaatggtt tgacctggat
    20401 aagcagtaaa attgaaatct tcgatcaaac gctgcttggg gtcataagaa aaagaaacat
    20461 gatcaaaaga gacttttcct ttgatatttt tgggttccaa ggcataggca tctggcaaat
    20521 caggcttttc ttctggctga tcgatcaaat caaatgctcg ttctagacca gaaaaagcgg
    20581 tttgcagttg tgtcgtgatt ccagaaagtt cgataaatgg tttagaaaac tgacttgagt
    20641 agatcgtaaa gctggaaatc acaccaacag tgattgacga accactcttc aatgccaata
    20701 ggccgccaac taaaccaatc gataaataag caaggtgatc tacaaaacga gaagacgggt
    20761 ttgtcaagga agaagaaaat tgtgcccgtt gtcctttttc atatagatca gcatttagca
    20821 gttcaaattg ctgttgacta gcttcttctt gctgaaaagc tttcacgatt ttctggttac
    20881 cgattcgttc ggtcacaaag cccgagatat ccccaataat cctttgctgt ctatcgaaat
    20941 ttgtttgtga agcacgagca acgatccaac tcacaaggaa aataattggt gtagaacaaa
    21001 gaacgaccaa tgtcagaagt ggacttagac gcatcataaa aataaacgaa agaatgatga
    21061 ttgccactcc agaaaataat tgattgaagg ccgcagaaat tgcaatggaa atattgtcca
    21121 tatcatttgt aaatcgactg acgatatttc cgtgggagtt ttgatcataa taacgaagtg
    21181 gcaatcgatt cagatgagca aatgcatctt ttcttaattt tgcaacggat aagtaagcta
    21241 accgattgct taacacttgg atgagccatt gactgactac gttgattaaa acgataccac
    21301 cgaatagaat aagtaaatgg aacagtcgat tgaagcggac ctgtccttct ccaatcattt
    21361 gatcgatact ttgaccaatt tgtaacgtca tcacaactgt tgcaatccca ttgatgattc
    21421 ctagtatcac agctagccaa atgtcttttg cataggcagt aaggtaagga atgaatcggc
    21481 ggagcgtctg caaggataat ttttttactt tcataactat tcttcctcct gtgatgcgac
    21541 gatttcctga tattctgctg attgagcaag taattcttca tgggtaccct gagcagcaat
    21601 cttaccttga tccatcacta agatatgatg tgcttcttga atagagcgga cacgttggga
    21661 gataataatc actgtactgc caagataagt ctgtagcgcc ttacgcaaat ccaagtctgt
    21721 ttgataatcc aacgcactta aagagtcatc taaaatcagt aatgctggct tggcaatcaa
    21781 agctcttgca atcgtcagtc gctgtttttg tccgccagag aaatttttcc cattttccat
    21841 gacttttgta tctaaaccat ttggaagctg acggacaaat tcttctgcct gagcgatttt
    21901 cagagcttcc caacattctt tttctgtggc gtctttctta ccccattgaa ggttttcacg
    21961 aatagtcccg gtaaatagta cagaagtttg gggaacaaga gcaatttgct gacggagatg
    22021 atccagctgc caagaacgaa catccattcc gtccattata actgtaccgg aactgacatc
    22081 atagaaacgc ggaatcaatt gtgtcaaagt cgttttcccg cttccggttg gaccaatgat
    22141 tccaaggact gtatttcgct tcagctgaaa tgtaatattt tggagagaaa gaccagaatc
    22201 aggagtataa cggaagtcaa catggtcaaa agcgataatt gcttcagaag aaaaatcggg
    22261 agtagtactg acctcagctt caagaatcga attttctgtg tctagcacct ctttcacccg
    22321 atttcctgaa gcttcggcac gtgtgaagat cacgacgagg tttgaaacaa cgatcagtgc
    22381 caaaagcatt tgattcatat aattgatcaa agcaagaact tgtccttgtt gcaagctacc
    22441 aatatttact ttgatcccgc caactgtcag caaacagatg atcccgacat tcatgatcaa
    22501 ggttgtagca ggtgataata gggcagagat attggaaaca cgtaaataat tgttcgctag
    22561 atcatttgtg cttttatcga acttctctgt ttcttttttt gtctgcgcaa atgcgcggat
    22621 gacacgaaca ccgctaagat tctgactaac gtgttcattg agctgatcta gtttttcttg
    22681 cactttttgg tagagaggaa cggttttttt gatgataaaa aataaaatga ttgagaaaat
    22741 aggcaataat gctaagaaaa acagacctac ttcccagtcg atcacaaaag ccatgaccac
    22801 tgacccgata cttaaaaacg gtgctctgat caataaacga atgaccattg ctaaagctag
    22861 ctgaagctga ttgatgtcgt ttgtcattcg ggtgatcaag gtatctgttc caaaatgatt
    22921 caactcgttg tgagagaaag tattgatttt tttgattagc tgattacgta actctgtacc
    22981 aaatccttgt gaagctactg aagcatagta ttggcaaatg atagcacaaa tcagaccaat
    23041 cacagacata gcgaccatcc ataagcccat ttcaatgatt tttcctcggt cattcatttt
    23101 tagtccatta tcgatcaagg aagccatcat taaaggcagg acaagttcga agacagcttc
    23161 aagaaatttg aagaacgggc ccaaaatgat ttgcttacga tagtcttttg cataacgtaa
    23221 taactgaatc atatgattcc tcctattatt tatttgtcca caattgaaaa aacaagtact
    23281 accacattgt agcttaggaa atttagaatg aaaagagaac aagccttttc at
//
LOCUS       DAZKNO010000027        23218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000027 DAZKNO010000000
VERSION     DAZKNO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.027"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            363..731
                     /locus_tag="ACG8MY_000788"
     CDS             363..731
                     /locus_tag="ACG8MY_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0430010.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="ACG8MY_000789"
     CDS             911..1852
                     /locus_tag="ACG8MY_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0430011.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="ACG8MY_000790"
     CDS             1921..2652
                     /locus_tag="ACG8MY_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0430012.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="ACG8MY_000791"
     CDS             2796..4976
                     /locus_tag="ACG8MY_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0430013.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="ACG8MY_000792"
     CDS             4996..5478
                     /locus_tag="ACG8MY_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0430014.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="ACG8MY_000793"
     CDS             5494..6255
                     /locus_tag="ACG8MY_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0430015.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /gene="murC"
                     /locus_tag="ACG8MY_000794"
     CDS             6386..7720
                     /gene="murC"
                     /locus_tag="ACG8MY_000794"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0430016.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="ACG8MY_000795"
     CDS             8027..8512
                     /locus_tag="ACG8MY_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0430017.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="ACG8MY_000796"
     CDS             8505..9188
                     /locus_tag="ACG8MY_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0430018.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="ACG8MY_000797"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="ACG8MY_000797"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0430019.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="ACG8MY_000798"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="ACG8MY_000798"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0430020.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /gene="coaE"
                     /locus_tag="ACG8MY_000799"
     CDS             12804..13433
                     /gene="coaE"
                     /locus_tag="ACG8MY_000799"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0430021.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="ACG8MY_000800"
     CDS             13485..13817
                     /locus_tag="ACG8MY_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0430022.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="ACG8MY_000801"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="ACG8MY_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0430023.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="ACG8MY_000802"
     CDS             14643..16016
                     /locus_tag="ACG8MY_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0430024.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="ACG8MY_000803"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="ACG8MY_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0430025.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="ACG8MY_000804"
     CDS             17071..17511
                     /locus_tag="ACG8MY_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0430026.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="ACG8MY_000805"
     CDS             17655..18356
                     /locus_tag="ACG8MY_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0430027.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="ACG8MY_000806"
     CDS             18353..19474
                     /locus_tag="ACG8MY_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0430028.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="ACG8MY_000807"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="ACG8MY_000807"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0430029.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="ACG8MY_000808"
     CDS             20853..21761
                     /locus_tag="ACG8MY_000808"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0430030.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="ACG8MY_000809"
     CDS             21796..22068
                     /locus_tag="ACG8MY_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0430031.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="ACG8MY_000810"
     CDS             22079..23125
                     /locus_tag="ACG8MY_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0430032.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7819 a         4042 c         5085 g         6272 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggtaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacat
//
LOCUS       DAZKNO010000028        23179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000028 DAZKNO010000000
VERSION     DAZKNO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.028"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..2026
                     /locus_tag="ACG8MY_000811"
     CDS             119..2026
                     /locus_tag="ACG8MY_000811"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0430033.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(2567..5845)
                     /locus_tag="ACG8MY_000812"
     CDS             complement(2567..5845)
                     /locus_tag="ACG8MY_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0430034.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(6116..6661)
                     /locus_tag="ACG8MY_000813"
     CDS             complement(6116..6661)
                     /locus_tag="ACG8MY_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0430035.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(6673..7365)
                     /locus_tag="ACG8MY_000814"
     CDS             complement(6673..7365)
                     /locus_tag="ACG8MY_000814"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0430036.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(7384..7668)
                     /gene="macP"
                     /locus_tag="ACG8MY_000815"
     CDS             complement(7384..7668)
                     /gene="macP"
                     /locus_tag="ACG8MY_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0430037.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(7681..8244)
                     /locus_tag="ACG8MY_000816"
     CDS             complement(7681..8244)
                     /locus_tag="ACG8MY_000816"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0430038.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            8472..9134
                     /locus_tag="ACG8MY_000817"
     CDS             8472..9134
                     /locus_tag="ACG8MY_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0430039.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            9258..10454
                     /locus_tag="ACG8MY_000818"
     CDS             9258..10454
                     /locus_tag="ACG8MY_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0430040.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(10555..11931)
                     /locus_tag="ACG8MY_000819"
     CDS             complement(10555..11931)
                     /locus_tag="ACG8MY_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0430041.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(11968..12834)
                     /locus_tag="ACG8MY_000820"
     CDS             complement(11968..12834)
                     /locus_tag="ACG8MY_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0430042.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(12923..13948)
                     /locus_tag="ACG8MY_000821"
     CDS             complement(12923..13948)
                     /locus_tag="ACG8MY_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0430043.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTVTLFFPIL
                     NFYNEKR"
     gene            complement(13966..14640)
                     /locus_tag="ACG8MY_000822"
     CDS             complement(13966..14640)
                     /locus_tag="ACG8MY_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0430044.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(14824..15717)
                     /locus_tag="ACG8MY_000823"
     CDS             complement(14824..15717)
                     /locus_tag="ACG8MY_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0430045.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(15791..17488)
                     /locus_tag="ACG8MY_000824"
     CDS             complement(15791..17488)
                     /locus_tag="ACG8MY_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0430046.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(17481..18794)
                     /locus_tag="ACG8MY_000825"
     CDS             complement(17481..18794)
                     /locus_tag="ACG8MY_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0430047.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(18794..23063)
                     /locus_tag="ACG8MY_000826"
                     /pseudo
     CDS             complement(18794..23063)
                     /locus_tag="ACG8MY_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
BASE COUNT         6585 a         5078 c         4099 g         7417 t
ORIGIN      
        1 gattgaaaaa aacaaatcag tttgattgtt cgaattttca attttttatg ttctatttgt
       61 taattccttc attttgtaag ataatgggca cacgaaacaa aaaaggagcg atcgatgaat
      121 gaacaaagaa cgattgaaac aagatttcta tgaagcagtc aatgaagaat ggcaaaaaac
      181 agcaaaaatc cctgcagata aaccagctac aggcggtttc caagatttag ttgacgggat
      241 cgaccaactt ttgatgggag aaacagacga tctgcttgcc catccagaaa aagtgtcttc
      301 agaaaaaatg aaacatttcc ttgcatatta tcaactggca aacgattatg accgaagaaa
      361 tgagttgcat gctcaaccac ttcaacctat aatcgaaaaa gttgaatcat tgcagtcttt
      421 cgacgaattg aatgagcaat ttccagaatg gctgttggat acattaccat tgccatttaa
      481 tctggatgta gatgcagata tgaagaatgc gcaaacgaac gccttctttg cttatccgcc
      541 ttctttattc cttccagata agacgtatta tgcatcagac catcccaacg gtgcacaact
      601 aataggcgtt ttctttgata tggctgttca gttattagag cttgccggca aagaaaaaag
      661 tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat aaattgattg ctcctcatgt
      721 aaaaagtgca gaagaaaacg cagattacag caaaatgtat aatccgcaat cttttgatac
      781 atttattaaa tacacagata aactggactt gaaaaaactt gtcacgggtt tgattgggac
      841 aactccagat aaagtgatcg taactgatcc agtgtacttc gatcatttga atgacatcct
      901 gacagaagct cattttccgt tattcaaaag ctggatgatc attaaaacag tacgttctct
      961 tagtggttat ctatcagaag aattccgcca agtcagcgga atctattctc gcgtattgtc
     1021 tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat taccttgcct ctggacaatt
     1081 cgatcaagta gttggcgatt actacggaaa aaaatatttt ggcgaagctg ctaaaaaaga
     1141 cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa aaacgtctag aagaaaatga
     1201 ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa ttaaataact taggtatcca
     1261 agtaggctac cccgataaga tcccagcatt attcgaccag ttcaaaacag tacctgcaga
     1321 tcagggtggt actttgcttt caaatactct tgttttcagc aaactcacat taaaagatcg
     1381 tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg gaaatgagtg ctgacacggt
     1441 caatgcttat tatcatccat ttcggaatgt gatcgtcttt ccggcagcga tccttcaagc
     1501 accattttac agtctagaac aatcaagctc tgctaattat ggtggaatcg gagctgttat
     1561 cgctcatgag atttcccatg catttgataa taacggcgct ttatttgacg aatatggcaa
     1621 cttgaacaac tggtggacag aagaagatct tacacaattt caaaacaaag cacaagcaat
     1681 gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa gtaaacggaa aattaaccgt
     1741 ttcggaaaat attgctgatg caggcggtct aagctgtgca ttggaagcag caaaaacgga
     1801 atcagatctt tctatcgagg aattctttgt caattgggca acgatttggc gtacaaaagc
     1861 aaaaaaagaa taccaacaac ttttactgca aatcgatgtc catgcccctg ctaaactccg
     1921 agcaaacgtg caaccaaaaa accttgacga attctatgaa acatttggta tcacggaaca
     1981 agacgctatg tatctagcgc cagaaaaaag agtgcgaatc tggtaagagg ttgtgccaga
     2041 agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat cgcttctcaa atttttgtga
     2101 catttcggct tatttttcga agaagttact tctggaatac catgtattag aggttgtgaa
     2161 aaagctgctt agtttcaagt agtaagaaaa attttgtgaa aatggctttc cacatttttg
     2221 cataaatttg acttactacc gagaaactgg cttttgaaca ccgtttatcg agaggttgtg
     2281 aaatcagcgt cctgacctga gtattagaga aaaaaacgga aaaatagttc ctcatatttt
     2341 gccgtttgtt gagctaatac cgagggtcag ctgattgaac accgtttagt ggagagattg
     2401 tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaagcagc aaggagaacc
     2461 tcatctataa aaagatagaa ggaagtgaga catctattgg tctcatttcc ttctattttt
     2521 actttcctca gtcaattgtt ttacatcata aaaggagaaa ttccacttat ttttttcgct
     2581 gtttcatgac ataaacaact acaccgatta acaaaattcc tccagctaat gctagcaacg
     2641 ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc atttttttca ctttcttttg
     2701 ctgtcctatc cggatgagaa ggttttccat ttggttttcc atttggtgat tcatttcctg
     2761 gattttctct ttctggtttg tcatttcccg ggggcacatc ggcagtttct cgcacagtga
     2821 tcgctttttg ctgggtaaca gattctgtat gagcagacca tttttcccca tcaaatattt
     2881 ccgaacgata agtgacttct agcgtatatt ctccttttga aggtgctgaa aaaatagcgg
     2941 tatgattaga aaattgagca cttgactgtg tttcaacgat ttggatcttt tctgggaaat
     3001 aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt gaaagttgac acaaaaactg
     3061 taaaaggctt cagttcaatg atatttcctt ctgcattctc cgccacacca tttgcttctt
     3121 gtataataat cggattattt tgttcatttg gttttgtctg atttggttct tgtacgattg
     3181 catactctgg atattccgaa gacggtgagg tttctccgtc aaaaatagca cctacagata
     3241 atggaagacc atccactctt tccttttctg tttgacgatt ccccggttta atctgcatca
     3301 gtccatgaac atggatactt ccattttcat agcgcgtaac aggctgctga ccttctttcg
     3361 aacatgtatt tagagactcg atccaatctt ttgatatggt atgtccaaga gtattttctg
     3421 attgtttctc tcgttcattc caatagtagt tcaacgagtg gtagatttga tcttcgtttt
     3481 gattcaatgg gcggatctgc tctctcatct caagattctc tgttcggaaa gataagatac
     3541 cagaagcatg gtagtctgtg tttcctgctg aagaggtata aagagccaca ttatatgaac
     3601 cgttattaaa acttgtactg ctattgacga taatatctgt tccgctattg gaatcgattc
     3661 ctttcgcaag attttcgaaa gacaaactat tgatcaattc atgagggccc gttaaagaag
     3721 aaccgcccat tttgaatcca ttaccgttac cttctccctc aattcctggt acccagccgt
     3781 ttcgataagc aactgagttt ttgatgatta cttttccgat cgaacctgtt tcatttttag
     3841 caaaaaggtc ccaaccgtca tctgcattat gatacgcgat acaaccgtca aatacatttc
     3901 cttctcctat tgtcagtttg gccgcaaatc catcggcatc ttcaaatcct ggatcggcat
     3961 tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg ccatgcttcg aaaggatcat
     4021 ttgagctacc gctgatttgg attcctgtat ttccattccg ataagcatga atctcttcga
     4081 tcaaattatg atttccagaa atctgtaatc ctttttgcat agctgcacta tttgtaatgt
     4141 caaaccctcg taaaccccag tagtgactga acagtgtcat cccatttcca gttcctttaa
     4201 aatcaagtac aggacgcttg ttttctggat ttttctctgc aatcaactga atcgggtttt
     4261 ctcttgtccc gttaacgcct tttggaatcg tcaatcctct ttcgaaggaa taagttccag
     4321 gcgccatgac gattgtctgc ccagcatatg cgtatctcaa tgcttgtgtc agagataatg
     4381 gattttgtct gcttccgttt cctgcatctg acccttctgg tgttacataa actgtcgaat
     4441 atttaggttt acggtagtcc actgtatgaa gaatctcttt tgtttcataa ctagtcatct
     4501 ctacgtattc ttctggcgga tagtttttat ctggcgtaaa actgaaagta aattcgttcg
     4561 ttccttccat cagttttgtc tgataatcaa attcccgatt cgcagtgata gccaagtttt
     4621 ttgcgttctt aagcacctct cctgttgctt tatttttaat tgtcaataat ccgtcagcat
     4681 ttgctctaaa ggacatagta tgatcttggt ttcctgaatg attactagag gtcacagtgt
     4741 aaatcggatc aatcaagcga gtaggtcttt cttgcccagg aggatctgtc gcaggatctg
     4801 agtaagcaac ttcgattcct tctaccttga tcgtcatatt ccttgctgca gcaaatcctg
     4861 cataaataac cgattcatct gaagcgtata atttttccca gtcatacatg atgtcagaag
     4921 ccatttcttc tcctgtttca ggatcgtaat aatatgcctc taaaccagta ttcgtttttt
     4981 tcatctctac gtccagttta gtaatcgagt tcgtggccgg aaacagattc ccatgaccgt
     5041 ttccgaatat attataagaa cctgtttctt tttccaagaa ccctgctgaa gtctctaaag
     5101 gtgttgtttc cactgagaca ctcccaggag ctggcgcagc ttgcggatct tcagatgata
     5161 ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt atatttgctg ccgctcgtgt
     5221 tgaccacttc ttgtctatcc gagtcccaga catattcgat ccgactgcct agtatggcaa
     5281 atgaatttga ataaaatgcc tgcccaaaca aatgattttt tggtactgcg tctcttacca
     5341 ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga ttctatttcg atcgtcgccc
     5401 gcatcctaaa gttgatgtct ttggggattg cttgataata ataggtcatt ccatctgatc
     5461 cgcttgactg gaattttccg ccgttattcg tggaagagag ccgcacgccg tcttctaatg
     5521 gttcaatggc atacctacta gcacttgtgc tttctccgaa aacaacccct ttccactctt
     5581 catttgcttc ttcagattgc ttttttgtct tttcattttc tgattctttc atcataggat
     5641 catttccttc ttccgtatcc atatcttcac tagttcttgg tatttcctcc attgttgaca
     5701 tcccttcaat gttcacgtgt gcattttctt cgttcacaga ctgttgttcg ttagcagcaa
     5761 cttcaacgac agaaattaaa accggggtac tgagcataag gagattcatc attagcgcac
     5821 tgtattttat acgttttttt aacaagtgta ttcctcccgt aacaaattag aaagcgttta
     5881 cttaaagtat tacctaattt atttttactg tcaataaaaa acaaaattat aataagtgta
     5941 ttttataaaa agaaaaaata taacttaagt tgttgataat aaagagattt attaacgtaa
     6001 aaagaataag tttttttaaa ataacgtata aaaaagagca taaaacaaag tcgtttgttt
     6061 tatgctcttc ttttattatt ttgtgctttt tattttatca aatcactttg tttttttata
     6121 aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc ccattcatgt ccaacgggat
     6181 cgaaacttgc cacggcatct ttgaaaatat ggagtttgta tcctagatta tacgcatcga
     6241 ctgctgtatg caacacacag atatctgtac atacgccggt caaataaata tctgtgatct
     6301 ggcgttctcg caaacggatg tctagatctg ttccactaaa agcggaataa tgccgtttgt
     6361 ctatccaata aacattttcc tcttgctcat agtcttggta taaacttttt aaagagccgt
     6421 acaattcccg tccacccgtt cctatcacat tatgcggagg aaataagcgg ttctccggat
     6481 gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac gacaaaatcc ttttgatcaa
     6541 taaacttctt tgtatgagca gtcagctttt tttcaattac ttgtcctgct gtcccagtag
     6601 tcagctttcc atcctctgca acaaaatcat atgtgtagtc aatcgagatc aatgcgttca
     6661 tcttagtcct ccttatacga gatgtttgac aaattcgatc accatttctg ctgatcgttt
     6721 gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc gagtgatctg ctgtgtcgct
     6781 cattgcacgt acgatcaaaa atgggatatt gaactgacga gctgtttgtc caacagcggc
     6841 tccttccatt tcgcaagcta atgcttcagg gaaattcgtt aagatttctt tgattttatc
     6901 tggcgagtcg acaaatgtat cacctgtaac gatcaatcct tcttttgcgt tcaaattcgt
     6961 tgcttttgct gctttaacca tttcactgcg caaatatgtg cttgcttcat aatatagagg
     7021 cattcctggt aattgtcctg ggttgtagcc aaatcctgtt gcatctgcat caaaataagc
     7081 tactttgtct gatataacga tgtcacctac ttgtaagcct tcaccgattc cgccagctga
     7141 acctgtattg atcaccatgt tcacgccgta ttgttggatc aacagacttg tggtaataga
     7201 tgctaagact tttcctatac cggaacgtac gacgatcact tcgtggttac ctaatgaacc
     7261 tgaaataaat aaagcgcctg cgcgttccca tgataatggt tcgcttaggt tctctctcaa
     7321 aatttttact tcttcttcca ttgcgccgat aataccgatt ttcatctttt taattccttc
     7381 tttctatata aatgcgattg ctaaaaaaac cacacataac attaggataa caataataag
     7441 agatttcatc aaaaaagagt tccatttggt cgtcttttcc cgttcgctga tccttgtttt
     7501 cgtgattggt ttattctttt tgctttcttt acgataaaag ctggcaatct ttttttcttc
     7561 ttgctgataa gctgtttctg cttttcgctg tttttttaat gactcgcttg cttgtgcttg
     7621 ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct ttggccataa gccgatccct
     7681 ctactttcct ttggtcaata gttcccagta ttgaatcgca taaatcgtct ttgcatcaca
     7741 aatcaactga tctttcatcg cttgttgcgc ctcttcaaga gttagatgat acaactccag
     7801 tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc tctacccctt gtgcatgata
     7861 aatatgaagg acttcatttg caaaacctgg tgaaagatac atcgaagtaa gatggctcag
     7921 cgacttcgca cgatagccag tttcttcttc taattctcta gctgctgtca tttcgggatt
     7981 ttgtccttct cctggatcga tttttccagc tggaatctcc aaaatcactt tttctaaagg
     8041 ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg tcaaaagcaa tgatcccgac
     8101 accgccagga tgaaatacca gttctctctt cgaagttcct ccatctggca aacgaacttc
     8161 gtctacagca acatcaataa tttttccgct atagatttct ttgcgactga ttgttttttc
     8221 ttcaaattgc tcattgttca gcatatcttc tcctgctttc tttgtaatca taactgcttt
     8281 tttttcattt ttcaattttt ctcaaaaact ccttattagt ttaccataga taaaccttag
     8341 attttctcta aatatagaaa atccttcttt agtccgatac tctttatagt agtccgtatg
     8401 ctaaaataag tatgtaatcg aataacgaaa agaattcaat actcaacgat aaatgacatg
     8461 gagggtttat tatgataaaa cgaaattggc cgtggcttct agcattggtc ttgctatttc
     8521 tctttgtccg aaacaatagt ttttcttttg ttctaggact tttattgata ggtgtagttt
     8581 tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg aaaacaagaa gaaattcctg
     8641 cactgactga cgaacttgaa actcactatg aaaaaagcgg tatgacagcg aatgaaattt
     8701 cattttttcg acaaacaatg aatcaaacaa aaaatgagat caatcaattg caaatgaata
     8761 tgcagcagac agccaaactt aaagcaatcg atctccgcca tgatccagtc aaagcagcga
     8821 aagctttatt caaagaattg gtgaaagaac caaaccgact gcatgaagca agtcagtttc
     8881 tttatacgca tctgccgaat ctagttgatt tgacaaataa gtataatgag atcaatgacc
     8941 atgagatcaa aaacaaacag acctatgaaa aactagaaga aagcgcgcag atcatcgacc
     9001 agctgtcagc tttaatcgca caagattatc aaaaattcgt tgctgaggat ctagatgata
     9061 tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg tgataataaa tatgaaccag
     9121 aacaaaagga ttaactcttt tgttcataaa agaagactcg gaacattcag atgatgtctg
     9181 gctgtttcga gtactttttc taaagtagag acattcatgt aagaatcgag aacagattca
     9241 acaaggagga attttttatg gatcataaac cgactgaaac aaaagatgtt acaccagtta
     9301 acgatacact tgaagattta ttgaacaacc cgttttcaac tccaacagat acgctgacca
     9361 cttcacaaca agcggagatt gatcagctcc aagagcaaca gacagctgct cgtttaattg
     9421 acaaactacc ggcggaacgc caagaacaag ctaaacagct agcagcaaaa atcgatgcta
     9481 atgatgcaca gtctgtcatc agctacggat cggctgccca agcaaaatta agtgaattct
     9541 cacaatcgat gttgaatcat gtgcaagcac aagatattgg tcctgtagga gattccttaa
     9601 ctgaactgat gtatcgtctc caagaagcca atcctgacga actgcgtgcc ggagaaggga
     9661 acatcttcca gcgtgtcttc gggaaggtca aacaatcgat ctatgaagtg acagcaaaat
     9721 atcaaaagat tggtgcacaa attgacaaga tttcagtgaa attagataaa gaaaaagacg
     9781 gtctgctaaa agataatctg atgttagaac agctttacca gaaaaacaaa gactactttg
     9841 atgcactgaa tatttatatc gctgctggag aattgaaaat ggaagaactt caaacaacga
     9901 tcattccaga agcgatgaaa agagcagaag aaactggcga tcagatggat gttcagattg
     9961 ccaatgatta cacgcaattc ctagatcgtc tggataaacg tacgcatgat cttcgcttag
    10021 ctagacagat cacgatccag caagcaccac aaatccgttt gatccaaaac acaaatcagg
    10081 ccttggctga aaagatccaa gcatctatcg ctacagccat tccgctgtgg aaaaaccaag
    10141 tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt aactgctcaa cgccaagtct
    10201 ctgaaacaac aaacgactta ttgaagaaaa attcagaaat gctgaaaatc tcagccatcg
    10261 aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga gacccttcaa accactcaaa
    10321 atgatttgat cgaaacaatc caagagacat tgcgtatcca aaaagaagga aaagaaaaac
    10381 gtcgtcatgc agaagtagag cttggacaca tggaagaaga tctgaaacaa aaactgttgg
    10441 atttaaccca atagatcacc tttcgcttgt tactaaaaca aacgcccaac aacagtttat
    10501 tacaactgtt gatgggcgtt tttcttatct ttcttctaac ttctccattg gagtctattt
    10561 ctcattcatc ttgctgtgtc catagccata gccaaaataa atgatcagtc caataaccat
    10621 tacgataccg aacacgatcc atgtcacagt ttgcagacga agcatcaata caatacaagt
    10681 caagatggag acgattggca atagcggaac gaatggcact ttgaattctc cagcttttgg
    10741 tttgccaaac atttttctca acttcagtaa gcctagacta acaagcatca aataggcgag
    10801 cgccatgata ttcgttagtt ccgctaatac attcaatggg aaaaatccgg caaagaatac
    10861 agctgccaca ccagcggcat aagtcgcttt tttcggtgta cgatgctttt gatcgatttc
    10921 tgtcataaac ttaggaagta agccatcctt gctgacagca aacagtaaac gagcaagtga
    10981 atacatcatc gagatcgtta ccgtcagtag cgttaaaatt gcccctacag cgatcaccgc
    11041 gcccactgtt ccatgtccta catacctcat tgcaaaagcc actgggtcat tgactcctag
    11101 ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg atgatgtaaa ggacagtagc
    11161 aatcagtatc gaaccaatga ttcctcttgg cacatctttt tgcgggtttt tgacttcctc
    11221 ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa aacacaagag ctgcaccatc
    11281 tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag ttatcaggtt taacgaaaaa
    11341 tattcctaca atgataaaca acgcgatgat accaaatttg acatatacca tggcattatt
    11401 caaacgtaat gctttcttgg cttcaagcga cacgatgtaa gtgacgaata tcactacgag
    11461 caccgcaatc aagtcaatgt atgtaccatt ttccggattg tacccgccag ttaatgcttg
    11521 tggaagttcg gcccctaaac ttttcaaaaa accttgcata tagccagacc atccggaagc
    11581 aacagaggaa accgctagta aaaattcccc gatcaacaac caccctgtca gccaggcagc
    11641 aaattcacca aatacaacat acagataggc atatggccca ccaatcactg gcactcgcga
    11701 agcaaattct gcgaaactta gtccagacaa aatgacaacg attgcagcta agacaaatga
    11761 aagtgacagt gctggaccag cattttgatt ggccgcaacc cctgtcacca caaatattcc
    11821 cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca gacgttttta gttctttttt
    11881 catctcactc gtatgattga gattgatctc tttcttacga aacaaatcca ttgaagttcc
    11941 tccccatttt aggttggaaa acagcgatta aactgttttc atatacccta aaaggatacc
    12001 acctaaaatg actaaaaggc aaccaacaat aacataaatg agctcttttt tcgtttttct
    12061 ttcgccaagg atgaaaatcc cacctaaagt agagataatg atccccattt gtgaaagtga
    12121 gaagccaaca gctaaaccaa gtgatttgac tgttagaagc atgcagatgt ttcctaagcc
    12181 ccataataat ccacagatca tgtttttcca cacaaactga tcgactttaa ctttacggaa
    12241 agcaaagaag cttgcgccta agatcattcc gatactttga ggtaagataa ttgacatcgc
    12301 atccaaattc gcccagttga cgatgatggt atatacacca taaccaatcg tagagaagat
    12361 caatgctcta aaacccttac caaaatcaag cattttatta tctgtatcta tttttccttc
    12421 gtcatcttgt cgggcagtca aatacgcacc gcttacaaga agagctaaag caataatacc
    12481 gtatacataa tctttcgttt gtgtccattc atggaagaaa acggctcccg caatcgtatt
    12541 caaaattaat tgaaaaccag tagacaatgg tagccctaca gaaacaccca tgtacttcat
    12601 tccatggaac tgtccatttt gtccaacact ccaagctaag ccggataaaa atccgatcaa
    12661 gaaaatccaa ggggtgatta caggactaac aacgaaaaag acaactaaag aaaacagaaa
    12721 tgcaccgatc gtcattccta gtgtttgttg attggcatcg ccaccaattt taccacttac
    12781 taaaccaata ctaccccatg ccaacatagg gactaatgct attaaaattt ccataaccaa
    12841 tctcctttta tttcacatga taagaaactt atcagcgaaa acgttaacaa cacaagcact
    12901 ttttttcgtt tctaacgatt ttctaacgtt tttcattata aaaatttaag attgggaaaa
    12961 ataaagtcac tgttgtccct tttccttcag ttgattcagc cgtcaaatgg atacctaatt
    13021 tttcggccag attttttgct aaataaagtc ctaatcctgt tgaatgtgtt ttgctaagtc
    13081 gaccatttct cccagtaaat cccttatcaa agatccgttt caagtcttcc gctgggatac
    13141 cgatccctga atctttaact gaaaggctga ctccttgcgc ttccttttct atgctcacaa
    13201 tgatgtttcc gtgatccggc gtatatttga tggcattact aagcaattgg ttgaagataa
    13261 aagcgatcca tttagcatcg gtcaaaacag tatgatcctc accaacgatc tcgtagcgaa
    13321 tatttttttg gataaaataa tttgcctgac tgcgtatcac aggctgaatg attcctttta
    13381 gtccatattc ttggatgagg tagtcccgcg agaaactgtc gagtcttgca taatacaaaa
    13441 cttgttctac gtattcatcg atttttgata attcattttc tagtaatgta tacttttgat
    13501 cgtcaatatc gaactcgatc gaacgaacta gcaatttaga agcggaaagt ggcactttga
    13561 tttcatggac ccatgagtca atataatctt tttggtcttg ttgattactg attgctgatt
    13621 gcattacttc ttggtgttca cggatcagct gattgatata ttcttctacc agtttttctt
    13681 cttctgtcct cccaccactc aaataatttt gcaaagggga agttttttgt atgtcggcta
    13741 attttcccca ccatcttctc ttgctcaagt aaccacccag cagataaaaa atcagaaaga
    13801 cgccctcaat caaagctaaa taagcaattg tgctcaaatc aactttgatg tttggcgcca
    13861 gccacatcac aaagatcgtc aatgcaataa aaaacagcca tcccaagatc aatgtccact
    13921 gatctttcaa atatttaaaa aaattcatct taaccgctca tcctttcagg gaacgatata
    13981 tccctgtcct acttttgttt cgatatatcc ttggatacct gcctgttcga tttttcggcg
    14041 taaccgattg atgttgactg tcaatgtatt gtcatctaca aatcgttcgt catcccacag
    14101 tgctcgcagc aatttttccc gacttaagat ctttccattg tgcttcatca aaataaataa
    14161 tagacgatat tcatttttgc tcaaatcaat gatctctccg ttgatttcca tacttccgtt
    14221 gtcaacattc aacgtaatgc cattgtggct catcatttca ctagataatt ctgtgtagtt
    14281 gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt acatcgattt ggaaatgttt
    14341 tgtcacaaaa tcatctgccc ccatgttcat agccatgatc atatccatat tcgtattcct
    14401 gctggagata aaaatgatcg gtactttcga tacttcacgt attttttgac accaataata
    14461 gccatccaaa acgggaagat taatatctag taatacaagc tgtggttctt ctctttcaaa
    14521 gtctgagaac acttgattga aatcggtcgt tccaaaggtt tcaaattgcc atttctcaag
    14581 ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg actaccatga tttttgccat
    14641 aaaaaatccc tccatctttc ttcttgctac tacaatatca aagaagccct tgtgaaacaa
    14701 gttggcttac ctggaaaatg tcattttagc aaacgttaaa aaaaactgga aatatgcgat
    14761 ttaatcgcgt ttgtctcttc caagcagctt aaatttatta tgttattctt aataattggt
    14821 ctattatctg ttatttttct ttcgttgttt ttgtctttta aattttcgat aacaccaata
    14881 accgaaaagc gttagcacta gcaatatacc tagaaggagt ataagtaatc gataaagatg
    14941 gtattttttt gtctgttcta tctttgaaga gacttcttct gtaaagggaa ctcgtcttcc
    15001 tgtcactagt aatcggtgag tgttcacggt gtaaggtgta caagtgataa gtgtgactag
    15061 atcctgcttt tctctaattg ctaatgtatt tgtttttgtt ggaaggacaa caattttctc
    15121 aacgacttca taagcgagca tccgaccata cacttcaaga tagaatcggt catcttgctc
    15181 catttcttca agatccgtaa agagtttttt ctcagcaata ccgctatggg cggtaatcac
    15241 ggaatgtgta ctctctcctc cagttggaaa agaggtgcct tgcaggactg tcgctcctct
    15301 atctaacaaa agatcattcg tttcatcaaa taaaggcaag ctaacattga ttttcggaat
    15361 atagatggct ccgatcatat gttgttcgta atattctttt cctggatttt ggacatttct
    15421 aaccgcttgc tcaaaaggat ctttgacttg tcccattcct gggattactg atgtatgagc
    15481 aagcgtctga tttttctttt catgcactgc taattgttgc tcatgttttt cttctttatc
    15541 gcgttcaagc tgcttttgat aattctcgat ccttctttga tccaaataat tgctgagagc
    15601 atctgctaaa aatggatatg taaaaactgc agctccggca agaaacatca ctgccataaa
    15661 taattgtaat aaaagactga tttgggtttg aggtctatta cctattttct ttttcatttt
    15721 cttcaccttc ttatttaaaa aaactgcagc aacctttata aaaccgattg ctgcagctta
    15781 tttttgttgc ttatacttca gacttcattt tagatttacg gtaccagctg tacgctccaa
    15841 tcatcaaact tattccgatt agtaagaatg ccaagagccc gttcccacca gtagcaggaa
    15901 gtgacccgcg tttcatattc ggtactcttt ctggccctgg attttctact cctacgatct
    15961 gaatttcttc attatagctt ccataagaga tgataaattc agtaaaggct gcctcacctg
    16021 ttggaagtac ataaccttct ggcgccatgg tctctttcat ttgataggtt ccataatcta
    16081 acccttgaac ctcaaagtat ccatttccat ctgcgacaaa ttctgttgct tgtccgcttg
    16141 ttgcccactc aattctatct gctcctgatt cataaacagc taaaggcgac tcccctttat
    16201 aaaatattgc gtattcagaa cggtcttcat tccacaattc gaatcttgca tctttaagca
    16261 attcactgct tgatgcatca tatttttcaa actttcttcc acctgttgtg actttctctg
    16321 gcggctctac tccttcttca taatcaaatt catcatttcg tccgatttca ataaccgctg
    16381 tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac cttcaaatta tacgtgatcg
    16441 ttaaatctcc tccacgtgca tctgctaatc tacctaaagt atctactttg gctactccat
    16501 tttcttctgt caaaatcgtc acgacaaacc cgttgcttac tacttctact atataatctt
    16561 gtccttttgt taaaatcgtc tcgccggctc gcacttctat ttcctgatca taataagtta
    16621 gaccaactgt aggcgtatct gtgattttgt aatttttgat aacagcgctt ccgttttcaa
    16681 ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt gtaagaagcc gtttttcctc
    16741 tttctagatc aatataagaa atcgtttctt gttctccagc ttcattaata atcgttactt
    16801 gtctaaggtc gctttcctct acattcatct gtttatctgc ttctctgatt tcgtttttcg
    16861 gatacaaatg cacatctgta ttgtatgtgt ctttccatgt agaaccatcc cacacgccat
    16921 ccaccatatc aggcatcatc actggcatca taacaaccat tggagacgcg attctggaaa
    16981 tatgagctgg cgaataagtt tctaagaaaa gatagacttt atctctagaa gaaggtgctt
    17041 cttccgtcac ttggacggta gagaataccg cttctccagc ttcattcgtt gtttgtttgt
    17101 caatacttgt cagtcgatat tttaaaaacc aactttctgt gtgccgtttt tgaatccatt
    17161 caatcgcttc ggcagaatta agaccgtctt ctcgttccgg tgttaaagga ttatctcgaa
    17221 tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa atcgactcct ggaatatttt
    17281 cttcaccaaa atccgtagaa accaatccgc tattcggtcg ttcttccgga acagatgaga
    17341 aacctttttt gtgtaatgta atcgtcactt cacttggttt ttgtgcgact tcttcggctt
    17401 tgactagttt tccgttcccg aaaatcccta ttcccgcagt aacgagtaac agtacactca
    17461 ttcccgtgac aatcattttt ttattcattt ttttctcccc gttctttttt tattaataaa
    17521 acagcaccta acaataaaag aattcctagt aaagaaatca acgttcttgc ttcattcgtt
    17581 tgcgggaata cggaccattt tcctgattga cctggattgc ttggaccact cggctctttc
    17641 ggtttgtccg ttgtttcttt tacggtctcg ttataaacac gtggtgttgc ggattcatag
    17701 gcagagactg gaactttccc ggattctaat tcagccatct cctgtccgtt aactactaca
    17761 tattgtggtt ggccttttgc atccacccaa aattcaggga tgatgacctc gattctctta
    17821 cttgcttcat ccatctcgta accttccaca ccttttaatt cttcaaaata ataggtgcca
    17881 gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt tatccgaaat gaatttgctt
    17941 acatttttgt cattcaaagg atcattgtct gctggtttga tccattgatt tttcaagtca
    18001 ttagcaggag ccatatctaa ataatatttc cccccttcta tcatctgata tagagcaaaa
    18061 acagcacctt ctaatggttt tcctttttca gttgttcctc tttgacgtcc atacttgaag
    18121 aagtatggat ctcttagata accgacattt ttaggataga tatgaatctc ttgcagttcc
    18181 tcggttgatt ctgtcgggtg ataaattggc aaaatagcag caattgggat agctgtctga
    18241 tcgatatcaa catccaatcc aacttctgga tcaatcgatt cttcgaaaat caaataaact
    18301 gcatcccgat cttggttttt cttaggtgct ttgatacgtg caatcccgtc tcctaatccg
    18361 ccaaccgtat ctttcccagt tgttaccgaa gtccctattt ggacaagcct ttcttctgtg
    18421 attagctgac ggatattgtt tctgttcgtt tcagcgattt gctggacgaa ttctttagga
    18481 gtcatgtttt cagcagcttt tttttcatga taataatcgg tcatgtcgta aatggcaaat
    18541 gtcgctccat ttagtggcac cgtctgtgaa atgatatctg ctgtttcccc tgcttcctca
    18601 gagatagcca agccatcatt ttcatggtat tcaaattgat cgttataatc gatatctcga
    18661 atcatccgtt tatgcaacac gaattccacg acatcttccc ctgattcttc tgccgacatc
    18721 gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa gtaattgaag catcaaacac
    18781 aacagctttt tcattacctt gctccctcct ttttagattg tctgacaaac aaaccatagg
    18841 ctgctcctgc aagcgccatg aagaatgcag caagcaggat atagaataat cgtccggatc
    18901 ctcctgtagc aggcaacgga ttttcaggat cgttcccgac actaagctgt aatctgattc
    18961 gatttgtctc caaaagttca tattctaact cttgctgtag atcaggcgtc tctgcttctt
    19021 cgtttggata atacgaaatc tcaactttta gtgtacccgc ttcttttcct ggaactaccc
    19081 gatcattttc tatgtcaggt tcttctcgat aactaatttc taaaataaag taacctggaa
    19141 gttttctata gctgtcaggt gacttcactt ccttgatccg atagacccct tccaacaagt
    19201 ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc caagcctccc tcgatgattt
    19261 catactctcc tgcactattt tgtttctcaa ttgaaaattc tgcgccttca agacgtcggc
    19321 tattcatact gtcaattttc aaaagttcca aatcgattgg cgctagttca tcttctaagg
    19381 ttacttgtag tacaccatcg atgatttcat agccttccgg caagggagca gtttcgtctt
    19441 cgatgaccgt atcttcaatg ataaacttac cttcttcatt cagtgcaaac tggaacactt
    19501 gatcagatag ccgataccca tttggtgcga cagattccga gacctcatat tcaccaatag
    19561 acagatgcag aggttggtcg taatttaaat cgacatactt ttctactcct acggcacgag
    19621 aatagaagta tccgatccca ggcaaacgtt tcgattcttc tggttctaac ggattaccag
    19681 tgtatgctcg atacagcggg aaggaagaat cccccacttt cctgatatcg aaacctgcac
    19741 catctaatgt atggccttca tggtctaatt ttctaactcg gatctcccga taatcaaacg
    19801 tattgtaagc agtgaatgct acccataaac gatcctcttc atctgttgta tacgtgagat
    19861 attgccaatt ttcttcacct gtttgcttga gttggatagt ccctatagca ttatcgtaaa
    19921 gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa accttctggg ttctggctct
    19981 caatgatttt gtattctgca tagttggtag tttcttcact atccttataa acaaatgaat
    20041 ctggctctcc gattggatga ataactttct caccatccac gattacatat ttcccgtctt
    20101 catctactga atagaacgtg agtctccctt tctcttcctc tacttcgttc acaccagaaa
    20161 gccctttcgc tactggatat ccgatttcca gtccgttacc atctgtttca taaagttcaa
    20221 actctgcgcc attgattggc atattactgc cttcaattag tttattgata ttcatgtgga
    20281 ttggcttgaa ttcatttcgc atttccagtt tgacatacaa ctcatcatac tctgtgcctt
    20341 ctatgttagg ctcttgtaca acagaaagct tgtgatattc tccttcataa taggaattcg
    20401 gatctttacc ttccggtccg ccgactctcg aaccagatgt atattcatct acaagctcgt
    20461 agtgacctaa catctcatct tcgttggctg catatttttt gatttggaat tgatacgtct
    20521 catctactaa ctgataacgt ggtggtccag cgatctctct gatccaaaat atgtcgctga
    20581 ctgctccagt ttcatctcct aaaatcggca tcttcgtttc aaagatcgct attccgtccg
    20641 tggtagtttt acttgtttcg attggttcgg cttccccact ctctggattt tggtcatata
    20701 atgcaaattc gactccagcc aacggctcgc cattttgacc cttcttgctg acttggaaat
    20761 tacggccttt atgttcgtaa cgaatggaag aagatgggaa atacatccgt ccagtagtga
    20821 tatcattctc ataatcctcc aggttctgaa tataggccat attgttcact gtgcaaaaat
    20881 ttccgtcttg agctcccgca tcaagtttta cttggtactt gatcctgacc atttcccctt
    20941 cacctaaaga aatcggagaa aatttataag cttggttttc ccgtccgaca tacgttgtcg
    21001 gagtcgccac tacttctggt ggggcttgtc catgacgttc ttcatcccat atttctggct
    21061 cttggtactt catgccgttt tctcctggat ctagatggta aaactgaata tctgccgatc
    21121 tgccagctcc aaatggtgag acattaaaac cagtttcatc ataaagtgtg gcttgctgga
    21181 tcgttctctt gaaggtagaa gagatatcct ccaaggctgt aacaatgtca ttcttgttgt
    21241 tcgcattgta atatcgcggt gttacgccat cttcttctaa atcagcaata tttttcaata
    21301 catttcgtcc aatagcatct agtcgttgtc tcccgctaac gtttccatcg attcctaaac
    21361 caatggagaa gatacttggc gggttctctt gatacttgtt tttcagccaa tgatgataag
    21421 caactgtgtt gatcgcagat gacttttctt gcgaagtagg actgccattc gtattgttat
    21481 tagaagaacc atcaccaacc cagtgagtcg ctttattatc tccttctttt acctctccat
    21541 aacgatagtg tacatttact tcatcttctg gacggttaaa gtcagatgca taggtaactg
    21601 gatgagtaaa ccctgcatta gaagtattcc cagaaaactc ctcaaaattt ctgaacaaaa
    21661 cgtcattatc ttcagcaatc ttatcttcca tcacagtcca gttgctccaa gctccattag
    21721 tgctcgtacg ataacttgac tgaatcggtg cataactgaa ggtcggtgtc ccatctccca
    21781 caacgattag gattttttct ggatttctat tttctccgcc attgtcttta tacaatgttt
    21841 catagccatt tttaagccct agtgtaagcg gagttcctcc gattggtgta cgatttaatg
    21901 ctgtattctc acttcccgtc aaacgattga tatcatttgt caatggaaag atgtccgaac
    21961 tgatttgctc atggttattg atagctgtac tattatggta aaaattcacc ataccgattc
    22021 gaatatccag actcgggtca gagagcagat ctttactcat ttcgtttact gcctcaatca
    22081 acgcagcatt tttagtttga ctattggtcc cagcggttaa ttcactcata ctcgctgact
    22141 tatctagaac aaaaacgata tcgattggac tgatttcctt tttctcgccc ccgatgacat
    22201 ctagatagac attgtaaagg gttgttgcat cttcaggatg tagatcagat gctgtgctgc
    22261 ttggcagaac aggttctacc cattttttga tgtacgcccc gttataatcc aagtaactgt
    22321 tttcaaaatt ccgttcattt ccccaaagct gaacggtttg agctggaacg tttctatctt
    22381 cttcgcctgc agttgatcca ttattagtag catagtcgta atttcgccag ttcgctcccc
    22441 cgtttgtata atcactatca tcgatcaagt tctctccgtc atatggcgag cctttgtctc
    22501 cttgatagcg ttcattgctt tgtgtccaat ttcctaacac ttttgtatag tcattcgttg
    22561 taaacttcat ctattctttc atcaaccacc acttgaagag gtagttaccc ttcgttcaca
    22621 tcggggatct tttctgtttc aaaaatcaat gtgccggttt ctgcttcttt tgaataaact
    22681 tttccgacat accattcttg accatcgatc gtctcgactt ttaggtcttc tccatcttca
    22741 tcataggagg atagacctgt tccacgaacg ttttttacgg taccaatccc ggcggctgcc
    22801 gcatcgattg ctaaacgtat tctaccttca ttggcacttt cctttttttc aaaagccaga
    22861 tgccattcaa gggtttcttc tttttcaata tacttccctt caatttttgc atagtcctca
    22921 tctaagaata cctcatcctc ctttgtttct gttgactcag cagctacatc cagggtcgtc
    22981 atatgataga ctgactggaa atatggtcca aatagcaaac tgacagttac taactgcaaa
    23041 agcttcaatt ttaacccttt cacatcctta tactcctttc aaaaatattt gtcttctttc
    23101 acaacaatca acaaaacaat gcccatctaa ttataattta atttaatttt aattttaaat
    23161 caaatgatat cctttttag
//
LOCUS       DAZKNO010000029        23137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000029 DAZKNO010000000
VERSION     DAZKNO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.029"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG8MY_000827"
     CDS             <1..1152
                     /locus_tag="ACG8MY_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0430048.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8MY_000828"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG8MY_000828"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0430049.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG8MY_000829"
     CDS             2728..3003
                     /locus_tag="ACG8MY_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0430050.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG8MY_000830"
     CDS             3009..3776
                     /locus_tag="ACG8MY_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0430051.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG8MY_000831"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG8MY_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0430052.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8MY_000832"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG8MY_000832"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0430053.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG8MY_000833"
     CDS             7920..8222
                     /locus_tag="ACG8MY_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0430054.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG8MY_000834"
     CDS             8225..9388
                     /locus_tag="ACG8MY_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0430055.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG8MY_000835"
     CDS             9414..12842
                     /locus_tag="ACG8MY_000835"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0430056.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG8MY_000836"
     CDS             12863..13987
                     /locus_tag="ACG8MY_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0430057.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG8MY_000837"
     CDS             13968..14414
                     /locus_tag="ACG8MY_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0430058.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG8MY_000838"
     CDS             14398..14736
                     /locus_tag="ACG8MY_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0430059.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8MY_000839"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG8MY_000839"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0430060.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8MY_000840"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG8MY_000840"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0430061.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG8MY_000841"
     CDS             15893..16936
                     /locus_tag="ACG8MY_000841"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0430062.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG8MY_000842"
     CDS             17095..17823
                     /locus_tag="ACG8MY_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0430063.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG8MY_000843"
     CDS             17939..18184
                     /locus_tag="ACG8MY_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0430064.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG8MY_000844"
     CDS             18181..18399
                     /locus_tag="ACG8MY_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0430065.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG8MY_000845"
     CDS             18535..19074
                     /locus_tag="ACG8MY_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0430066.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG8MY_000846"
     CDS             19067..21340
                     /locus_tag="ACG8MY_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0430067.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG8MY_000847"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG8MY_000847"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0430068.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8MY_000848"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG8MY_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0430069.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
BASE COUNT         7743 a         3937 c         5001 g         6456 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattt
//
LOCUS       DAZKNO010000030        22688 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000030 DAZKNO010000000
VERSION     DAZKNO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.030"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACG8MY_000849"
     CDS             127..3654
                     /locus_tag="ACG8MY_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0430070.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACG8MY_000850"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACG8MY_000850"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0430071.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACG8MY_000851"
     CDS             7442..7783
                     /locus_tag="ACG8MY_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0430072.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACG8MY_000852"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACG8MY_000852"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0430073.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACG8MY_000853"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACG8MY_000853"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0430074.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG8MY_000854"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACG8MY_000854"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0430075.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACG8MY_000855"
     CDS             12994..13731
                     /locus_tag="ACG8MY_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430076.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACG8MY_000856"
     CDS             13745..14560
                     /locus_tag="ACG8MY_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0430077.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACG8MY_000857"
     CDS             14573..15220
                     /locus_tag="ACG8MY_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0430078.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACG8MY_000858"
     CDS             15236..15892
                     /locus_tag="ACG8MY_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0430079.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACG8MY_000859"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACG8MY_000859"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0430080.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACG8MY_000860"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACG8MY_000860"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0430081.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACG8MY_000861"
     CDS             18234..18752
                     /locus_tag="ACG8MY_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0430082.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACG8MY_000862"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACG8MY_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0430083.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="ACG8MY_000863"
     CDS             complement(19639..20559)
                     /locus_tag="ACG8MY_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0430084.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20685..21737
                     /locus_tag="ACG8MY_000864"
     CDS             20685..21737
                     /locus_tag="ACG8MY_000864"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0430085.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="ACG8MY_000865"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="ACG8MY_000865"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0430086.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7562 a         3954 c         4827 g         6345 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaat
//
LOCUS       DAZKNO010000031        22089 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000031 DAZKNO010000000
VERSION     DAZKNO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.031"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1861
                     /gene="efbA"
                     /locus_tag="ACG8MY_000866"
     CDS             155..1861
                     /gene="efbA"
                     /locus_tag="ACG8MY_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0430087.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="ACG8MY_000867"
     CDS             complement(2019..2921)
                     /locus_tag="ACG8MY_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0430088.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3610)
                     /locus_tag="ACG8MY_000868"
     CDS             complement(2918..3610)
                     /locus_tag="ACG8MY_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0430089.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="ACG8MY_000869"
     CDS             complement(3653..4294)
                     /locus_tag="ACG8MY_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0430090.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4646)
                     /locus_tag="ACG8MY_000870"
     CDS             complement(4332..4646)
                     /locus_tag="ACG8MY_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430091.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4634..5263)
                     /gene="pyrE"
                     /locus_tag="ACG8MY_000871"
     CDS             complement(4634..5263)
                     /gene="pyrE"
                     /locus_tag="ACG8MY_000871"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0430092.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="ACG8MY_000872"
     CDS             complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="ACG8MY_000872"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0430093.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5983..6915)
                     /locus_tag="ACG8MY_000873"
     CDS             complement(5983..6915)
                     /locus_tag="ACG8MY_000873"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0430094.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="ACG8MY_000874"
     CDS             complement(6912..7688)
                     /locus_tag="ACG8MY_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0430095.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ACG8MY_000875"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="ACG8MY_000875"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0430096.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /locus_tag="ACG8MY_000876"
     CDS             complement(10932..12008)
                     /locus_tag="ACG8MY_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0430097.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="ACG8MY_000877"
     CDS             complement(12013..13293)
                     /locus_tag="ACG8MY_000877"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0430098.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="ACG8MY_000878"
     CDS             complement(13312..14238)
                     /locus_tag="ACG8MY_000878"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0430099.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="ACG8MY_000879"
     CDS             complement(14251..15534)
                     /locus_tag="ACG8MY_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0430100.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ACG8MY_000880"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="ACG8MY_000880"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0430101.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="ACG8MY_000881"
     CDS             complement(16322..17233)
                     /locus_tag="ACG8MY_000881"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0430102.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /gene="lspA"
                     /locus_tag="ACG8MY_000882"
     CDS             complement(17230..17694)
                     /gene="lspA"
                     /locus_tag="ACG8MY_000882"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0430103.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="ACG8MY_000883"
     CDS             complement(17699..18397)
                     /locus_tag="ACG8MY_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0430104.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="ACG8MY_000884"
     CDS             complement(18384..18770)
                     /locus_tag="ACG8MY_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430105.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="ACG8MY_000885"
     CDS             complement(18917..20587)
                     /locus_tag="ACG8MY_000885"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0430106.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20770..21003
                     /locus_tag="ACG8MY_000886"
     CDS             20770..21003
                     /locus_tag="ACG8MY_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0430107.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21102..21533)
                     /locus_tag="ACG8MY_000887"
     CDS             complement(21102..21533)
                     /locus_tag="ACG8MY_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0430108.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21612..22025
                     /locus_tag="ACG8MY_000888"
     CDS             21612..22025
                     /locus_tag="ACG8MY_000888"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0430109.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         6255 a         4929 c         3897 g         7008 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttaaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacga tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg tttttaattt
    20701 atttgacgta cttataaata ctctttataa tgaaggctat gatcagactt tagaaaggtc
    20761 gtgagtgctt tgaccacagg aattgtaaaa tggtttgaca acaaaaaagg atacggcttt
    20821 atcagttatg atgacacaga agaaattttt gtacatttta ctgcgattga agaagaggga
    20881 ttcaaaactt tggaagaaaa tcaagtggtc gaatttgaga tcatcgaagg gaatcgagga
    20941 acacaagctg cccatgtcaa gaaaggcaac gttcagacta ccgatactag tatgcatgaa
    21001 tagataaaca aatagagtga gacgctaaac gggatgaacc gtacgtctca ctcttttctt
    21061 ttcctgtaaa aattcgtttt tttaggttct gatttttaaa attagctttt tctttttttc
    21121 gtataaggat aaggaattga ttctgatttg ggtccgtttt tttcaaaatg atcaaaaagg
    21181 actacttgat ctttttttcg catcatgaaa acagatgggg ctttctcatc ttgcaacatc
    21241 atttgcaccc cagtattagt tggcgtcaaa gatcgaatct gtccgacaga tatttctcgg
    21301 tctttccaaa aagcagcata acgtataatc acagagtttg attttaaaat aaaagaacgc
    21361 cgcaaaccta gaaatagaaa aacaagaaaa atacctaaaa caaaattgct tttccaataa
    21421 ggacctgtgt tttctaaaga aagaatcaga ctaaaaaaca gaatgatcaa ggtcaatgac
    21481 caatagatga tcgtatgtgc cagttcaggc tgccatcgaa tatttttttt cattctctaa
    21541 cctctcaccg attcagtaat gatataatct taccataaca ttagtttgaa caaaaaggag
    21601 aatctctttt tatgttaaaa gtatacattg atgcctctac aaaaggcaac cctggtccta
    21661 gtggaggtgg aatcttgatt gtctatcaag gtaaacaaga acagctatct atcccgcttt
    21721 ctatagggac aaaccatcaa gcagagttcg aagtcttttt aaaaacttta gaatttctaa
    21781 agaaaaacga cttacagaat gaaacgattt tctgtttttc agacagcaaa acactagttt
    21841 ctacggtcga caaaaacgca acgaagaacg agctcttcct tccctacctt caaagaatcc
    21901 aagaacttct atccgaattt tctttattga tcctgcaatg gttgcccgaa aacaaaaata
    21961 aaggagcaga taatcttgca cgtcaggctc tccataaaca attaaaagaa aaaaagactc
    22021 tttaaattaa aaactttaag gagtcttttt atagtttctc atttaatata aaagataatt
    22081 gtgatcaaa
//
LOCUS       DAZKNO010000032        21859 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000032 DAZKNO010000000
VERSION     DAZKNO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.032"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..343)
                     /locus_tag="ACG8MY_000889"
     CDS             complement(<1..343)
                     /locus_tag="ACG8MY_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0430110.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLI"
     gene            514..1470
                     /locus_tag="ACG8MY_000890"
     CDS             514..1470
                     /locus_tag="ACG8MY_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0430111.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1543..1659
                     /locus_tag="ACG8MY_000891"
     CDS             1543..1659
                     /locus_tag="ACG8MY_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0430112.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1714..3396)
                     /gene="recN"
                     /locus_tag="ACG8MY_000892"
     CDS             complement(1714..3396)
                     /gene="recN"
                     /locus_tag="ACG8MY_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0430113.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3414..3863)
                     /locus_tag="ACG8MY_000893"
     CDS             complement(3414..3863)
                     /locus_tag="ACG8MY_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0430114.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3970..4788)
                     /locus_tag="ACG8MY_000894"
     CDS             complement(3970..4788)
                     /locus_tag="ACG8MY_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0430115.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4797..5687)
                     /locus_tag="ACG8MY_000895"
     CDS             complement(4797..5687)
                     /locus_tag="ACG8MY_000895"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0430116.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5689..5913)
                     /locus_tag="ACG8MY_000896"
     CDS             complement(5689..5913)
                     /locus_tag="ACG8MY_000896"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0430117.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5918..7255)
                     /gene="xseA"
                     /locus_tag="ACG8MY_000897"
     CDS             complement(5918..7255)
                     /gene="xseA"
                     /locus_tag="ACG8MY_000897"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0430118.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(7256..8110)
                     /locus_tag="ACG8MY_000898"
     CDS             complement(7256..8110)
                     /locus_tag="ACG8MY_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0430119.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(8207..8656)
                     /gene="nusB"
                     /locus_tag="ACG8MY_000899"
     CDS             complement(8207..8656)
                     /gene="nusB"
                     /locus_tag="ACG8MY_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0430120.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8649..9074)
                     /locus_tag="ACG8MY_000900"
     CDS             complement(8649..9074)
                     /locus_tag="ACG8MY_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0430121.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(9320..10384)
                     /locus_tag="ACG8MY_000901"
     CDS             complement(9320..10384)
                     /locus_tag="ACG8MY_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0430122.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10556..10801)
                     /locus_tag="ACG8MY_000902"
     CDS             complement(10556..10801)
                     /locus_tag="ACG8MY_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0430123.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10940..11314)
                     /locus_tag="ACG8MY_000903"
     CDS             complement(10940..11314)
                     /locus_tag="ACG8MY_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0430124.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(11397..12311)
                     /locus_tag="ACG8MY_000904"
     CDS             complement(11397..12311)
                     /locus_tag="ACG8MY_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0430125.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(12332..13135)
                     /locus_tag="ACG8MY_000905"
     CDS             complement(12332..13135)
                     /locus_tag="ACG8MY_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0430126.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(13168..14157)
                     /locus_tag="ACG8MY_000906"
     CDS             complement(13168..14157)
                     /locus_tag="ACG8MY_000906"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0430127.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(14260..14751)
                     /locus_tag="ACG8MY_000907"
     CDS             complement(14260..14751)
                     /locus_tag="ACG8MY_000907"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0430128.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14942..17749)
                     /locus_tag="ACG8MY_000908"
     CDS             complement(14942..17749)
                     /locus_tag="ACG8MY_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0430129.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(18183..18476)
                     /gene="rpmA"
                     /locus_tag="ACG8MY_000909"
     CDS             complement(18183..18476)
                     /gene="rpmA"
                     /locus_tag="ACG8MY_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0430130.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18489..18833)
                     /locus_tag="ACG8MY_000910"
     CDS             complement(18489..18833)
                     /locus_tag="ACG8MY_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0430131.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18860..19168)
                     /gene="rplU"
                     /locus_tag="ACG8MY_000911"
     CDS             complement(18860..19168)
                     /gene="rplU"
                     /locus_tag="ACG8MY_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0430132.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(19188..19264)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19450..20091
                     /locus_tag="ACG8MY_000912"
     CDS             19450..20091
                     /locus_tag="ACG8MY_000912"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0430133.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(20153..21784)
                     /locus_tag="ACG8MY_000913"
     CDS             complement(20153..21784)
                     /locus_tag="ACG8MY_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0430134.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMLQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         6353 a         4827 c         3645 g         7034 t
ORIGIN      
        1 tgatcaagac agcgtacaag acgattgtca acagaaaaac caaacgccaa gtcatcttga
       61 aataacgcgg ataaataatt tcttcataat aatatgcttg gaaaacagca atacaaattc
      121 ccattagcaa aacagaaata aagaaaaacg gtaagataga aattgtccct gtatttctag
      181 acaatgcatg cataccgccc cacaaaaaag gaatcgccaa atcaggggct tttattttaa
      241 aacgacgatt taacgaaaat aattttacaa gaaattggca accaaacaaa acgattaccg
      301 ggtaaatata ccaaaacaag ataagtggtg tgaatgtgcc catgaacgta aactcctttt
      361 ttatcgttgc ttcaagtata ctgtatatac tttacacaac tgtttcattt tatttaaatt
      421 tatgcagtta tattctatat cttacaaaca cattccatgt attccaattc cctatccgtt
      481 ataataaaag aaacaggcaa caggaggaaa atcatgatca aatattttac attagataat
      541 gagaaactgc gtaataccgg aaaggaaact gaccaaacga tctggtattg tgtagaacgt
      601 cctaatcaag aagagattca tcagctgaca catcaatttg aaattccgtc agattacatc
      661 acttccatat tggacgatgc agaaaattcc cgtacagaag aattcaatca atcaaagttg
      721 acaaagccag cgttattatt gttacaatat ccttttgcta aaacaagtcc tagcggttat
      781 tttcaagtgg atacataccc gctctctatt attttgacgc ccaaaaagaa gcttatcaca
      841 attacgaatc atgaaccact gtttttaaag aaagtcttta gccagacctt tccaaaaagt
      901 gatatttcgc ctaatctcaa tatcttcttt caattcattt ggcaggtcac actctcttac
      961 aacagttatt tagctacgct tcaatctcaa tgcgatactt tggaagccaa acttcaagta
     1021 tccactgaaa acaaacaact ttatcaaatc atggatatcc aaaaaagcct cgtttatttc
     1081 agagaagcga cttctgctaa tttagatgca ttgaaagaac tagcgcaaaa taaacttttc
     1141 caagaaaatc atgctttgaa aaatcattta cgtgatgtat tggtggaaac agagcaggca
     1201 atgactactt ctcgcattca attaaaacta gtagaacaaa tgaatcagac tttttcagca
     1261 atcgtatcga ataatctgaa taatattatg aaaattttaa cttctttaac gattatttta
     1321 actataccga caatcgtcgg cagtatctat ggaatgaaca ttaagttacc tattgctgaa
     1381 agggatgatg cctttatatg gctagttcta ttaacattat ttatttgtat cgtaactacc
     1441 tattatttaa aaaaagggaa gtttttataa aaaagccctc ttgtggaaat ggaaaaaaga
     1501 ttgtacactt gaatcaacta attaatgacg aggttgaaaa caatgcgtga aaaaaaacaa
     1561 acaagtactt ttgctaaagt aacaaaattt gtcatttgga caatgctgat tttaacgatt
     1621 ggatctgttg tattgactgc tgtaatgagt gtcatgtaaa aataaaatat agctatcagc
     1681 tgctgaaaag gacctgaacc actgcgaggt ggctcaggtc ctttttgcga gtttcaatag
     1741 ttcttttgca tgttcgagtg tgagttttgt aatctcactt ccagccagca tacgtgcaat
     1801 ttcttcttct ctttcttttg ttttcaactc tgaaacagaa gtttgcgtac gtccatcaat
     1861 cactgctttc acaatataat attgatagtc ggcgactgca gcaacttgag gcagatgagt
     1921 gatgcacaaa acttgagagt atttcgatat ttgagaaatt ttatctgcga tggcctgtgc
     1981 aactcttccg ctcacacctg tatccacttc atcaaaaata atacttgtca caccttgctc
     2041 agaagaaaaa atagttttca aagcaagcaa catccgagac agttcgccac cagaagcgac
     2101 tttcaccaaa ggcttcagtg gctctcctgg attggtcgta atgtaaaatt cgatttggtc
     2161 aaatccatca tgattcaatt gtctattttc ttcttttaag aaacgtacct caaactcagt
     2221 gttttccata tacagacttt tcaattcatg cagaatggat ttttccaatt cttttgcgat
     2281 ttttttacgc gcttcatgaa gatctgcggc ttgttgctga agtagaagct ctttttgctc
     2341 tagttcctct ttcattttgt ccaattgacc ttctgaaaaa tcagaagaag ctagctcttc
     2401 ggtgatttcg tcatagtaag ccaatattga ctcaatggat tcgccgtatt tacgtttcaa
     2461 ctgtcggatt aattctaaac gttgcgtaac ttcttctaaa cgactctcat ctaactccaa
     2521 taaatcaatc tgacgactca tatcgccgat tgcatcttgc agcaaatagt aagcactttg
     2581 gatattttca taaattgctt catactccac atctagatgg gcgatccctt gcagttctga
     2641 aacggccaag ccaacaccat ctacactgtt ttgttcatta ttcgtcagtg ctccgtaacc
     2701 tgcagctaat ccatcagcaa ttttttgata attgctaagt ttttctcttt cttcttttaa
     2761 tttctcttct tctccgactt gcaaatcagc agcagctatt tcttcttgct ggaaatggag
     2821 catatctatt ctctgaacat aggatttttc attttgctgc acttttcgta cccttttttc
     2881 cagctcacga taagagatat attcttgctg ataactcttt ttcttctgtt ggaaagctac
     2941 atctccaaaa cgatccaata aagcaagatg tgattcaggc tggagcagtt cctgatgttc
     3001 gttttgtcct tggatatcta ctaaataact gccgattctt ctcagattag ctaatgtgat
     3061 aatatgacca ttcactcgac aaacattttt tccagttaag gacatatccc gtcgaacaat
     3121 cagattgtcc tcatctgttt cgatccctaa ttcaaccatc agttcagaga atccttcttg
     3181 ttttggtagt tcaaacaacc cttcaagcac acatttttct gctccttgac gtatataatc
     3241 gcttgaacct cttcctcctg ctaacagacc taaagcatcg ataataatgg atttacccgc
     3301 accagtctct ccggtcagcg cggtcattcc ttcgtgaaaa gaaagccgca attccggaat
     3361 aatcgcaaag ttagtaatac tgatttcttg tagcatcaca ttcgctcctc aacttaaagg
     3421 taatgcagga agtctttctt caataaaact gcctggtctt cagttcgtgt aatcatcaaa
     3481 acattatcgt catcattgat aatcgaaaat aattctttct tgtaacgttt atcaatcaaa
     3541 tttgctgctg ccgaagcatt tcccggcaat gttttcaaaa taacaaattt ttccatttgg
     3601 tcaactgcca cgaaagcgtc ttttaatagt ttttccaatt tagtatttac atcttcgttt
     3661 gtttcagctg gtatgctata acggtagccg ccatctgaag ccggtacctt gatcagtttc
     3721 agttccttta tatctctgga aatcgtagct tgagtaacag atattccttt gcttttcagg
     3781 atttcaacaa attcttcctg ctttcggata tcttgctcac tcaacaatcg agtaatcaaa
     3841 cgatgccgat ctttttttct cattacgtct tcgctcctta gcatattctt cttaatcaat
     3901 gatagcttat ttccttggta taataaagaa gaatccgcat acttctcact tgattttcac
     3961 agttacaatt tatttatggt caaactgttc gtgtgcattt gaaaccactt ctgccatatt
     4021 aatagctgaa ttgattgttc cgctttcggg tactttcctc aagtgagcca agaactcgat
     4081 atttccttct ccccccgtga tcggagaata acttaattcc agcacatcat atccaccttg
     4141 tgccgcaaat gacaaaatgt cttctaaaac tttttgatgg gtctgagggt cacgaacaat
     4201 tccgtttttc cctacagctt ctctgcctgc ttcgaattgg ggtttgatta aagcaatcac
     4261 ttctccctct tcttttaaaa tagcatgtaa tggaggcagc atcagtttta gtgaaataaa
     4321 agaaacatcg ataacagcaa cttctggaat tcctaaggta aagtcttcgg gttttgcata
     4381 acgaaaattc actctttcca ttacgacgac tctaggatct tgtcttattt tccaagctag
     4441 ctgattgtag ccaacgtcta aagcataact catttttgcg ccattttgca aagctgcatc
     4501 agtaaatccg ccagtggatg caccaatatc taacaatgtt tttccttcaa cagatagatc
     4561 aaatgctttg agtgcttttt ctaattttaa tccccctcga gaaacataag gaagtttttt
     4621 acctttgact cttaattcac tactcactgg aattttttct cccggtttat caaaccgctc
     4681 gtttttttcg ttgtaaatca aaccagccat cactgaacgt ttggcttgtt ctcttgtttc
     4741 aaataatcct tgtttgaccg ctaatacatc tacgcgttct ttttccattc tttccctcat
     4801 ttctccacat caaattgctt gatcatctgg cgcatcagcc cgccatcaaa agaaaaaacg
     4861 tcctcttcta atttgtctat gatcttgttg gcggataaca gctcaatctc caacgcctca
     4921 cgtgtctttt ctaggcctaa taaacgcggg taagtatttt tttccatacg ttcatcttta
     4981 ccagctgttt tccctaatgt tttcgttgta ccaatcacat cgagtagatc atctcttatc
     5041 tgaaaagcta aacctaaatg ttcggctaat cgttgaagta gcaataagat ttcttcaggt
     5101 tgcttcgcta atatgccacc agccagcaag gcataacgaa tcaaatgccc tgtctttctt
     5161 tcatgaataa atgctaattc ttctaaagaa agctttttcg attcgccctc gatatctgca
     5221 gcttgcccag caaccatacc ttgtgatccc gcacaaacag ctaattgctg aagcagcaga
     5281 agcttaggag aattacctaa gtgtgccata ctgattagct ggaatgctcc tgtaagcaaa
     5341 ccatctccgg ccaatatagc taatgcttcg ccgaatactt tatgattggt aggttttcct
     5401 ctacgaagat catcattgtc catcgcaggc aaatcatcgt ggatcaagga atacgtatgc
     5461 accatttcta aagcagctgc tacttgatag acgggaacat caataggctc atcaaaggaa
     5521 gcaacggccg ttaaaagtaa taagggacgt attcttttcc cgcctgcttt cactgaataa
     5581 ctcatcgcat catagagtcc tctatccgtt gtatgctcat ctaaaaaaga caagatttct
     5641 ttttctacag caggtagatg aatagctgaa aagtcagtta atctcatctc agctttcctc
     5701 gtttccgtca aaatcaactt cttcatttga ttctgtcatc attttcgtca aggtctcttc
     5761 tgctttagct aatgtatctt ggcaatgttt gcttaattcc attcctcgtt taaacgaatc
     5821 tagagctgat tctaatggaa cgtctccttg ttctaactgc atgacaattt tttctaattc
     5881 ttgcagtgac tcttcaaaag ttggattttc catattttta ttctcctttg tctatctctt
     5941 gtacttgtcc tttaacgatt ccatcttgat aatggatcgt cagcaaatcc ccttgatgaa
     6001 tctcggtgac actttttact acttgatcat ttaacgtcgt atagctgtag cctcttccca
     6061 tgattttcaa tggactcaac aaatcaagag attgaacagc ttgagcaaaa cgctgctgct
     6121 gttgtttcat atattgttgg attttctctt ctaagcgttt ttctagataa gcagtttctt
     6181 ggcgagctgc tttaactcga taaagcggag ccacttgttc caattggtgg cttaatgcga
     6241 tcgtttgttt ttctttcgca tgataaattt gctgtgtagc ttgatataat cgctgtttca
     6301 actggtctaa tttgatggtc tgaccttcat acaatctttc aggctgccga aatacatagg
     6361 aacgtcgagc acgctcgaac cgttcttgtt tgcgctggat ctggcgtaca aacgcctgct
     6421 gcaatctagc ttgccgttct tgaatgcgca tcaactcttc tgacaaaaca ggaacagcta
     6481 attctgcagc tgctgttgga gtggcagccc gaacatctgc tactaaatca gcgatcgtgg
     6541 tatctgtttc atgtcctaca gatgaaatca ccggcgtatt agcttgataa atcgcccgag
     6601 caacccgctc ttcattaaac ggccataaat cctcaatcga accaccccca cgaccgatga
     6661 tcattgtatc gaaattgccc atctcttcta ctctctggat attcctgaca acattgtccg
     6721 ctgcttggtt tccttgtaca actgttggga acaaaaccaa ttggacaatt ggatatcttc
     6781 ttttcaccgt cgtgatgata tcgcggataa ccgctccgct tggactggta aggaccgcga
     6841 ttcgctttgg gaaacgtggc agcatttttt taggcccttg aaaaaggcct tccttcatca
     6901 gcttttcttt tcgttcttcc aattcttgat ataaagcacc tattccatca ggctccatgt
     6961 gctctacata gatttgataa gaacctcccg cttcatataa tgaaatacgc ccaacgacaa
     7021 ggactttcat tccttctttt ggctggaatc gtaatttttg gaatgctcct ttgaacataa
     7081 tagcagatat ttttgcatgg tcatctttta agctgaaata ttgatgggca ttggcacgta
     7141 aacggaaatt cgatatttcg cctgttaaat atacgcgttc taaataagga tcggcatcaa
     7201 attttctttt caaatacttc gttaatgtag tgacagttaa atattcttgt gccatctatt
     7261 cacctgctcc aaaagcttcg gcagatttta cggtttgata catcagcatc gtgatagtca
     7321 ttggccctac accctttggc acaggagtga tccagcctgc aataggctcc acttcatcaa
     7381 atttcacatc accgatcagc ttaccgttag catcacgatt catccctaca tctatcacta
     7441 cagcacctgg ttttacgaat tcttttgtga caaaatgtcc acgtcctatt gctacgacta
     7501 aaatatctgc ttcttgtgcc agttcagcca gattttcggt tttagaatga gcaacagtaa
     7561 cagttgcatc tgccatcaag agtagctgag ccatcggttt accgacgata ttgcttcgtc
     7621 cgataactac tgctcgttta cccgccggat cgatagaata agcttcaaac attttcatga
     7681 tgccgtatgg agtacaagga atcttgtccg gtttacccgc aaggagtctg cctaaattca
     7741 tcggatgaaa accgtctacg tctttttcag ggcggatagc taacaggatc ttttcttcat
     7801 ctatatgctt tggcaatggt aattggacca atataccatg aaaactagga tcttgattgt
     7861 atttttcaat ttctgcaagt agttcgtctt cagaaatcgt ctcaggataa cgctctacca
     7921 atgaatggat tccgatttct tttgctacac gttctttatt tcttacatag atttggctgg
     7981 ctggattttc ccctaccaat aaaacgacta atccgggatg gataccatta tctttcaatt
     8041 tttggatttt ttcagcaatc tctgcctgca ttttttcagc taattctttt ccgttaatca
     8101 attctgccat ctaaccactc ccagatattt tttgtgtttt cattctacca tagaattttc
     8161 ccgatttata acaggtttat gatgattcat agaaaaaagc taggacttac gccccagctt
     8221 ctaattcttt caatacattg gataaaacac cgttgacgaa tttacgtgaa cggtcatcac
     8281 taaatgtttt tgccaactcg attgcttcat ttaaagcaac agccgctggt acatctgtca
     8341 cataagtcat ttcaaaaatc gcgatacgca agatcaccaa atccattttt gagatccgat
     8401 tgatgtgcca attgtttttt aaatgttttt caatcacttt atccaattca tctttttttg
     8461 cacagacacc gccaactagc gtatcaagat aaacaggaac aaactctgat tcatcttcgt
     8521 tgatcatgtc gcggtggtct aattcgattg cgttgaaaat cgcatcttct tttgttaggt
     8581 ctgcattgaa gtctaatgga aataaagcct gcaatgccat ttcccgaatc tcgtgtcttg
     8641 ataattcttt attcattctc ttcctcttcg tcttcaaaaa gttcttcgat tgttggttgt
     8701 tctaattttt caggaacaac agctactaca tggatattga cttcagataa atcgatatcc
     8761 gtcatgaaaa ggacttgctg gctgacccgt tcctgcattt ccattgctac tttaggaact
     8821 gaaacaccat attctagata acagtaaaga tctactttca agccatcttc ctcgtttgct
     8881 aagtaaacac cttttccgtg agctgcacgt cctaataatt cagtgacatt gctggcaaaa
     8941 gttccgcgca ttccgtatac gccttctact ttagaagctg caatcccgat aattacttcg
     9001 atgacttcag gagcgatcac gatctctcct aaatcttggt ttgcgtttaa aatcaaattt
     9061 ttttcatctg ccatttattt attcctccct ttgcctaagc acgagataca tccgaattct
     9121 ctatgatgcg gcatagtgat cacgtggtaa gaaaactgcc gccgatcagc tgcaaactgc
     9181 tatgaattgg agaaaacacc caactttatt atctcttaca gcttaaaagt tcttctatag
     9241 tgtatcatta aatcgagtga ttttctataa caaagtctgt gaacactgat ttttacgctt
     9301 acttcttcta ttgatgaaac tataagatga tcaattcttt tggtgaacga gtcaacacac
     9361 gatttccatc ttctgtgatc aacaagtcat cctcaatacg tactccaccg attcctggta
     9421 agtagatccc tggttcatca gtgatcacat ttcctggtac aaattgcttt tctgcacgag
     9481 aagaaacgtt tggtccttcg tggatctcta aaccgatccc atgtcctgta gaatgtccaa
     9541 atgcttcccc gtaaccatgt tttgaaatat aatctctagc aactgcatct aattggacac
     9601 cagtcattcc tggttttgca gcatcaataa ctgctaattg agcatctaag actatttgat
     9661 aaatttcttt taatttttcc ccaggatcac caatagcgaa tgtacgtgtc atatcggaaa
     9721 cgtacccttc gtaatagcaa ccaaagtcca atgtgatcaa gtcaccttgt tcgatgattt
     9781 ttttgcttgc tacaccatgc ggcatagccg aacgcaatcc gctagcaacg atcgtatcaa
     9841 aagaaacacc tgaagcgcca agagaacgca tataaaagtc tagttgattt gcgacttcaa
     9901 tctcagtcat gcccggttgg atcattttca ggatgtgatc atatgccata tccgcaatat
     9961 gacatgcttt ttcgataata gcgatttctt cttcatcttt cacttctctg agttcttcga
    10021 tcatctgtga aacaggtact aattccgcat cgatgatttc ttctaaaacc gaatattcta
    10081 aaaatgagac tgttgtttcc tcaaaagcta atgagtgcag ttcttctttg cgtactaagt
    10141 ttgctacctc atcaaaaata ggtccgacat ttttaataat ttcaaaccct tgagcctgcg
    10201 ctgccgcctg ctcggtataa cgaaaatcag taataaaaaa tgctttttcc aaagtaataa
    10261 cagctaatcc tgtcgtacct gtaaagttcg ttaagtaacg aagattgtac ggactggtaa
    10321 ccaaaaatga atccaaattt tcttcttgca tttttttgcg caatttttct acacgtagca
    10381 tcataatgaa actccctctt tctttgcacg cttttttcca ttatagcaaa cttttcttcg
    10441 tacacgacag ttaaataaaa atttccggaa aacttctttt tattttaaat aaataaagcc
    10501 aaaaaggagt cacagtctcg ctgcaactcc ttttagttta ctattttgta aaaacttatg
    10561 atcgttttcc aaagaaaaat gagacaactg ctaccaaaat cactgcgcct aaaatagatg
    10621 gaataagtgc catacctgca gcttttggtc cccattcgcc gaaaatcgct tgtccaatac
    10681 ttgaaccgac tagtccagca atgatattcg ttacacagcc catcgaattt cctttactgg
    10741 tgatcgcacc tgcaattgct ccgatcacgg ctccaacaat caatgtccat aaccaactca
    10801 taatataacc tccttctttg ctaatcaatt ttagcatatg ggatttaaag gggacaaata
    10861 attggttttg ctatttcata ttttcttctg caaagtaaaa aaataaagaa aacctcctct
    10921 tagaagtttt ctttattttt tacttgcgtt taaacatact acgaatacca cgtttcacta
    10981 catcaaagaa actcaatgat tgtaccattc tatcttcatc tacatattct ctgatcgcac
    11041 gaagcacttt tttaggttct ttggaagaaa atgtgtatgt accatttttt ttcgtttgaa
    11101 tggcatatcg aggaatccat tttcccttta gcatgactga tgctagtaca taatccactt
    11161 cttcccatgg aatttggata tatttgcgtc tatctcgcgc atggtaaaac tcaaaaccat
    11221 tatcacctat catgatttgc ccgtaatctg tcagacccgt aaacgaagta gcatcaatta
    11281 ccaagtctac ttttgtattg attgattgaa ccattttctc cctccagata tttgtttatg
    11341 aacagtatac tatataaaaa aagctcggga caacgcccga gctatatttt tatggattat
    11401 aaaagtccga tgacgtgtcc aacaacaccg actacgaata aaccaaggat gataacgatt
    11461 ggagaaacct ttttcttaag caaccacata cataacaatg ttaatcctaa tgcagctaaa
    11521 ccaggaatca attggtctaa gttgtcttgt aatgttgtta ccttttcagg agaaagggct
    11581 aagccattat taacttgttc aaatgcactc ttgattcctt caccacctga aggcaattta
    11641 tcccattcga tgtaagcacc tttatctaat tttacagttg atacaactgg taagaatttg
    11701 attgataccc atctttgtac aagggcagct aatacgaaca tccctaaaat tgaagcacct
    11761 tttgttacgt cttgtaacaa tccgccagaa aggtcatcag taattttaga accagctttg
    11821 tagccaaatt cttgtgtata ccacatgaat gcccaacgaa taatgttcca acctaagaag
    11881 aagatgattg gtccaaggat atttcctccc atagctaatg aagcacctag tgcacctagc
    11941 attggacgta ctgtaaacca gaacactggg tcaccaacac cagctaaagg acccatcata
    12001 cctactttaa ccccttgaat cgctacatca tcaacaggcg cgccattcgc acgttcttct
    12061 tccagagcca atgtaacccc taaaattgga gaagcaatgt atgggtgtgt attaaagaat
    12121 tccaagtgac gttttaatgc tgcagaacgt tcttcttttg ttttgtataa ttttttgatt
    12181 gctggaatca tagaaaatgc ccagccaccg ttttgcatac gttcgtagtt ccatgaacct
    12241 tgaatgaagg tagagcgcca cgcaacagct aaacgatctt tttttgataa tttgattttt
    12301 tcttctgcca ttttattttc ctccttcaag attaatagtc attcaaaatg tcgcctaaag
    12361 ggtcaccaga gttgcttccg cctccattat ttgatgaacc acccatttta gaaaggttca
    12421 agtaaataag agctaatgca acacctaaag cacctagagc aatcaatgtt aattgagaaa
    12481 ttgcagctac aacgaaacca ataacgaaga atggccatac ttcttttgta gccatcatgt
    12541 tgattaccaa agcgtaacct acagctacga ccattccccc accgatagcc ataccatctg
    12601 ttaaccatgc tggcattgat tcaaggaatg attgcacagt ttgtgctggg ataaatagca
    12661 acgctgctgc tgggaccgcg atacgaaccc cttgtaaaca tacagcaatg atgtgccaca
    12721 tttcaacctt acggatgttt ccttcttcag cagccgcatc catcaaatga acgattggaa
    12781 cagcgattgt acgtacgatc attgttaaga acaaccctgc aacagccaaa ggaacggcaa
    12841 tcgcgatcgc agaaggaaca cctttaacac cttgtccacc taaaactaaa ataattgctg
    12901 atgctactga tgctaatgca gcatctggtg ctacagcggc accgatattt gcccaaccta
    12961 aagcgatcat ctgaagcgta ccgccaagta cgataccagc ttcgaggtta ccggtaacta
    13021 aaccgatcaa tgtacatgcc actagtggtt gatgaaattg aaattcatca agaatacctt
    13081 ccataccagc tagaaaggca actaatacga ctaaaattat tgaaataata gacatgataa
    13141 gcctcctata aattttaata ttatttatta cgcttttgct aattcagctt ttgctttttt
    13201 caaaatatca tccatgttat cgcgtgaatc attaggtact ttacgtacat cgaatttcac
    13261 acctttttgt tcaagcttct caaacgcttc aacgtcttct tgtcccatag acaatacttt
    13321 acttacaacg actttaccga ccgaatgcgc cattgaccca acatttaatt ctttgatgtc
    13381 tacgccacct tcaacagctg tcaatacgtc ttcaggattt tcaaataaaa gcaatgcttt
    13441 ggtatttcca aaacgagggt cttttgctac ttcgatcatt ttgctaatag gaataacatt
    13501 tgcttttaca cctggaggag cagcttgttc gattagtctc ttacgcagat catcctttga
    13561 tactgcatct gaaacaacga taatacggtt tggttgcact gcttttgtcc aagctgtcgc
    13621 aacttgtcca tgtaacagac gtgagtcaac acgtgctaat acatatttga tttttccgtc
    13681 tccgaccaca gttccttctg gaagtgctcc tttaggttgt gcgtcttcaa ctgctgcttt
    13741 cggtgcttct gccggttcta attcttccgg ttttactttt acaccgtctt ttgctgtttc
    13801 aatgatgtgt gctgcaattt catgagcaga ttccattgaa aagcgtgatg cgtaggcttc
    13861 aatcaacatt ggtaaattca aaccgctgac gattgcccat ttatctttgt gctcttcgaa
    13921 taatgtgttt gattgattga acggtgttcc gccccataaa tcaactaaga ataacacttc
    13981 gtcttgatta tcaaaagaag caaccgcttc ttccaatttg gctctcaagt catctggacc
    14041 ttcacttggt ttcaaaatca cggctttgac gttttcttgt tcgccaaaga tcattgaacc
    14101 ggattgtaag attccttcag caaattgtcc atgacttgct aggataatcc ctaccatatt
    14161 tatacctcct atttactttt gttgtaacaa attcaaaaat attaataatt gctaaccatt
    14221 acaacagaat tatttgacct tttccaacaa ccattcttgc tacacctttt tgattttcgc
    14281 tagtagttcc atcaaaagga ttttcctgtc agcaggtact tttcgaatct ctaactccac
    14341 ttcttgactg ttcaagtaac gaaaggaatc aatatcttgt tgatttactg aaacaaaatt
    14401 ggtaatcatt gatttgcctt cagtaaaact cataccgccg atattcaacg acttcaactg
    14461 tacaccgcag cggacaaccg cttcaacatc ttggggattg gtgaataaaa gcatcacttt
    14521 tgctcgtgat aataatccac tatgatagat ttgaatcatt ttctgcacgg ttattacatt
    14581 tgctgtgatc cccggcggtg cagcttcttt tagtagaaac tttctcaatt tatcttgagc
    14641 gactacatcg ctgatcacaa tgatacgttc aacacctgtt tgcttcgacc aaactgttgc
    14701 gacttgcccg tggattaaac gatcatctat acgtgccaat cgaatatcca tctgcaaaac
    14761 tccttcttcc tcactacttc acagaaaccc ctatcatttc tgtgtgaaca accacctcct
    14821 agcacacact actataagca aaaagcgtgc caactttttt acgatacact aattgaattt
    14881 aaaggattct taaccaatac acaaaaacga tacactataa aagtgtatcg tttttgtgta
    14941 ttcatggcac agtgtattct tttttatcta actgattatg gactagacgt agcagataat
    15001 acatttcttc tatcggcact tttactttta tctgatcttc caacagaaca actgaggctg
    15061 ccaattggct ataaagagga tcattcgttg tatttgtcaa ttcgttttct tccacagtca
    15121 atggttcatt ccgaataata cgttcgatca ttcctgctgt gtgcatcact aggttgattt
    15181 tgaatccata ttcttcatcg ccgattcccg tcgtctgaga gatttgtgta gaaaattgcc
    15241 acattggatc gattaactta ctaccattaa taaagatgaa gttatcagag ataaactcaa
    15301 cacacgtatt tttagctgac tcttcatcta gggagatgaa ttcagtttcc tccaattctg
    15361 attccatcag caactgatcc aaaatgactt cgatattttg atcaataaaa cgttctaaag
    15421 gaataaatgg ggcttggata ttcaaatcag taatcccagt agtagctatg atctgatatt
    15481 ctttttggat aactggcagt tcttctttca cttccacaat cgacaaagct agaacttcta
    15541 attctgtttc ttgacgcttg tttaccgctc gttcgatcaa ttctttgatt ctttgtgctg
    15601 taccttcgcc agatgcgcag atagccaaaa cagctttttt cttatgaata ggttttgctt
    15661 cctgaacaga ttctacagaa gatatttctg cgtaacctcg aaaatttttc aatgattcat
    15721 gcaacatatc tagatctgtc cctaatactg agacctttcg tacagtttcc aaaactagag
    15781 aagttgttac catatcaaca gtacgtactg gaattccagt gtctctttga atttgcgaat
    15841 taaacgacgc aagggacccc atatctacta agagtatgac cccgcttcct tcatctactt
    15901 cttgtacatt gcgttcaatt cggtccaatg ctgttttcgg atccatatcc aaaggcatgt
    15961 ctactgctcg tacgttctct gcatctaaca actgttggac aacttggacc atactgcttg
    16021 ctgtgctgtt tccgtgagct gcaacgacta caccgattct gccgcttgct tgatttgccc
    16081 gtaacgagac gagtagaacg gtcaagtaat agtcttcact ttctggaatc tccacttgaa
    16141 aatacgtttc gatataacta cgcacttctt tcgcaacagc atattcatct ggaaaatcaa
    16201 aagccatctc acggatgtta tcattcgtat gccgttcttc tcctaagttg atttttttca
    16261 aaaaagaact gatatgcaag ctcatagcat aaataaagtt ttgctgaaac tcatatggta
    16321 aagatctttt gaccattaca taaatctgat tggtcacttc tatgattttc ggatctacaa
    16381 attctgctaa tttgctgtct gcattaaaag aaaaaccatg gtctttatag aaagacttca
    16441 gatggatgtt gatatctgta gaaatgaaat gattgattgc ttcttgatcc aaaccatccg
    16501 acttcagtaa agctgcttta tctccgatga tatcatatag attataaggt aactcatagg
    16561 agtccgattg gattttcatg atcgtatcat ttggtgctac agttatcttt ggctccaata
    16621 ttttagaaag ctctgccatt gcactgcggt tgcttgctaa ttgaatcaat ccgctacgaa
    16681 tcccttctgt caaatcatcg attgtgatcg agatttcggg tgattgcata tgatttagaa
    16741 atcctcgtgc acatactaac tggatatttg acttcaattg tcctatattt ccataggtca
    16801 cactgccgat caatgctttt gctacatctt ctgtcaaaga aatctttcgc tgcgtgcggt
    16861 ttgcttcgtg ggcaatcatg atttttacta aatcgacttt ctcatttgca ggacgttttt
    16921 caaaagccgg aagtttgata ttgattggga tacgccgaac aaaagtttct aatagagaag
    16981 aacctgggtc ttctgtggtc gcacctacga tccgtacatt tgcttcatgg gatttcgttg
    17041 tttccccaag acgactgtaa gttccatgat ccataaagta aaagatcatt tcttgacctt
    17101 caggcggcaa ccggtgaatc tcatccaaaa acagcatacc gccatccgct tgatcgataa
    17161 tccccatttt ttcttcatct gcgcctgtaa aagcaccttt tgcgtaccca aataaatgac
    17221 tcatcaaaag ctcagggtta ttggcataat cagcacaatt gaaaacaatc agttccgttt
    17281 cctcatctat tacatgatgc gcttttgcaa aatgatacat cgcgtgtgca aaataagtct
    17341 ttcctgatcc ggtaggtcct gtaatcaaac aatttaatcc tcttggcgga tacaaaatcg
    17401 cagcttttgc ctgttccact gaatttttca tgctgccgtt agctccaatg atttttgcaa
    17461 agatatcctt agtatctatt tggagtgtat ttttttcttc agagaccggc gtttctttgt
    17521 aactgactac atgttttgta actggcttta cgtgcgtaac gagtgtttga tatatgtaac
    17581 gtactggtcg tccgtctgtc tttatcagtt ttccttcgcg cactagctga ttcagatctt
    17641 tactgctatt tgttcgctga atccctaaca cttcagcaat ttcctgtgtt gttactcctt
    17701 gatcatattc tgcctttgtt aaatgagcgg ttttttcttt gacatacata taaattcggt
    17761 caatgcgttt tgccaatggt atcgctcctt ttctatagac tattgtactt aggcttcaaa
    17821 acataatact atgtatttat ttctgtttcg tcaactattt acctgatttt gttttgttga
    17881 taacacgcgg atagatagaa atgttcaatt taaaagaacg aaaaatcccg atacgatttt
    17941 tataatggaa acaagaaaaa gaacactgtt taccatataa ttatttcact gatagcaaac
    18001 aatatagctt ccatcaattg aaaacgatag ttatcaaaac atcctgattc caaattggca
    18061 aaaaaatatt aagtgtaaaa agacaaagta aacttcgtag cctccactac aacttttatt
    18121 tttataaaca aaaaaggctg tgacaaaagt cttgtcacaa cctctttaat cataagattc
    18181 aattaagctt cttgagctac tggataaaca gacacttgtt ttttgttacg gcctttacgt
    18241 tcgaaacgta ctacgccatc aacttttgca aataaagtgt cgtctccacc gatacccacg
    18301 tttacacctg gatagatttt tgtaccgcgt tgacggtaaa gaattgatcc acctgtaact
    18361 gtttgtccat cagcacgttt agcacctaga cgttttgatt ctgagtcacg gccgttagat
    18421 gtagaaccgc cacctttttt gtgggcaaat aattgtagat tcatagataa caacatggtc
    18481 tgcacctcct atttatcatt aattgtttta atttggatat attctgagtt ttcagcttct
    18541 atcgattgta atcctaataa aagattttct aatagaatct cagcaatatt gttttgttct
    18601 tgtgtcattc ctgaagtgac ttcggtataa agataaccgc cttcttcttc attcatttca
    18661 acaatcggct caaaaccggc taatgaggca atcccgttga cagtgctgat cgctaacgca
    18721 gaaacacccg cacaaacgat atcacttcca taaggaccag catcagcatg cccggtcaaa
    18781 gtgaatgaaa cgatctgacc tgcgtcattt cgcttaaatg ttcctttgat catatatagg
    18841 ccttctttct caaacgctat taagcgttga tcgcgtcgat aaccactttt gtatatggtt
    18901 gacgatgacc ttgtttacgg tgtgaatgtt ttttaggttt gtatttgaaa gtaacgactt
    18961 tcttttgttt gccgtgtttt tcaacagtac cttcaacagt tgcacctgca acagttggag
    19021 cgcctacttt tgtagattct ccgcctacta agataacttc gtcaaaaaca actttttctc
    19081 ctgcttcaac gtcaagtttt tcaacataga ttgcttgtcc cacttcaact tttacttgtt
    19141 taccaccagt tttaataatt gcgtacatgc tataaagcac ctccttctga tatacttaga
    19201 ctcgccatcc gaagtgacag catatgctgt gcttattact tgttctgagc ggttgtagct
    19261 gtggagtcca caattacaac agtactagct taccagaaca gcaaccgtta gtcaactaaa
    19321 tttaaaatca aaaagtaaaa caaaaattca agcaatactt ctgcttttct atcctcacag
    19381 ttctaccaaa taaaatagtc gcattgtgtt tcttttattc ttataataaa agaaaaagga
    19441 ggagcataaa tggaataccc aaaagaactg atacaagaag cttctttaag aatgaacggc
    19501 cgtcctgtag aaggatttat cgctgggcaa ggacctcttc atcctaaact gatgctcgtg
    19561 ggagaagccc cagggaaaac agagattgat acgcatgttc cttttagcgg tcaagctgga
    19621 aaagaattga tgcaggcact ttcttcaact ggcttaacac gagaagaggt gtatatcacc
    19681 agtgctgtaa ggagccgccc gtatcgcgtg actcatcgga tcaataaacg gacacaacag
    19741 tcagaaactg tttatccaaa tcggacacct actcgttccg aagtatttgc gcatgcacca
    19801 atattggatt acgaactgat gaaggtccag ccaaaattaa ttgcgacact tggaaacatt
    19861 ggtcttcaac gtttacttgg caaagaatat acggttacca aaaatcatgg tcaactctat
    19921 acagggcctg tcttacaatt aacagaacaa aaggatgcct acgaatggtc agaaaaaaac
    19981 tatcgaattc ttccactttt tcatccagca gctattttct acaatcgctc ccttgtacct
    20041 gttatccatc aagactggca agtactagct tcacttttac agcaatatta aaaagaacag
    20101 acaagaccat aaaatcttca gtttacagtc ttgtctgttc tttttcattg ttttaatggc
    20161 taattgcagt atatttcgca tgtaaagctg tcaacgcatg agcaactgtt tgatgttgat
    20221 gcgtgaatgt atgttcccac aagtgagaaa gttgtttttc tacttctggc tgcaaggaag
    20281 tatagataat cgtttgtcct ctttgctctg ctttttcttg aaattcatca aaaactgcac
    20341 gtgccgtcaa atcaatactt ggtacgcctc gcatggagaa aatgacacca tcgtgatgat
    20401 ttagttcttc caatttattt ttgagttttt ctgaagacat aaaaaacagt ggaccactaa
    20461 tatacactac tgtccagtta ttcagctttt ccgtttctgg cagccccatt ctttgccaat
    20521 cgatcgtttc taatgaaatc gtgatagcag cgcttttgac aacaaaaaat acacagccgc
    20581 cgatcacccc gatcacgata gcaatactta aatcaaagat aactgtacaa atcatcgtaa
    20641 ggataaacaa tagaactgct gaccaatatt tattagtaaa tagctccttg atcgtttccc
    20701 attcattcat acgccaagcc gtcacaatca aaacacctgc aagtgctggc attggaatct
    20761 gtaacatcac tggcgcaaaa acaagcatgg agagcagtaa gacaactgca tgtatcatac
    20821 cagctaatct agtttgtgca ccagatttga tggccacgct tgtacgggcg atggccgcag
    20881 tagcaggaat cccgccaaag aaaggaagca acagatttcc aattccttgc gccattaatt
    20941 cttgattgct atctaagggc ttatttgcca ttcggccagc cgaagcacca catagcaagc
    21001 tttcaatcat tcctaataaa gcaatgctaa tagctggaac tgccacttgt tgcaaggcag
    21061 aaaagctaaa atctcccata ttcaaacgat tactactgat caatgtttgt ggaatcttac
    21121 ccacagttgc tactggtaaa tggaacaata gcataactgc tgttgccaaa atgatagcca
    21181 gcagcgaact aggcattcgt ttcgcccatt tttttggata gataaacatc cctaaaatga
    21241 ctagccctcc catgatcaaa gaggctgtgt ttatcggaaa tcctatcgtt tgataactac
    21301 ttagtttttc aataacgttt gtaccttccg agtgaacacc aaataaatta tcgatctgac
    21361 ctaatgcaat aatgatagca atacctgaag taaaccccgt aattacgggt gctgggataa
    21421 aagaagtcag tgttcctaat cggaaaagtc ctgctgcaag taaaaataga cccgctaaaa
    21481 aagtagcaag caatacacct tgcattccct ttgttgccgc aatagacatc aagatagctg
    21541 ccatcgcacc tgtcggacct gatatttgat aaaaaccgcc tgataaaccg ccaataacga
    21601 taccggcaat gatagccgta atcaaacctg cggccgcatc agctccgctt gataccccaa
    21661 aagccaaggc taaaggcaat gctacagcag ctactgtgat cccagccaaa agatctttct
    21721 gaaatcttga aagatcataa cctgaaaatt ctttttttag tgaggaaaca taatttttga
    21781 acacctatct aactcctttt tcatgaaatt ttctaaaata ccattcatta gaatatcata
    21841 tttcatgaga taaagtaat
//
LOCUS       DAZKNO010000033        21335 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000033 DAZKNO010000000
VERSION     DAZKNO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.033"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(123..2504)
                     /locus_tag="ACG8MY_000914"
     CDS             complement(123..2504)
                     /locus_tag="ACG8MY_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0430135.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGAGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2494..3141)
                     /gene="recU"
                     /locus_tag="ACG8MY_000915"
     CDS             complement(2494..3141)
                     /gene="recU"
                     /locus_tag="ACG8MY_000915"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0430136.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3205..3744
                     /locus_tag="ACG8MY_000916"
     CDS             3205..3744
                     /locus_tag="ACG8MY_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0430137.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3834..4253
                     /gene="gpsB"
                     /locus_tag="ACG8MY_000917"
     CDS             3834..4253
                     /gene="gpsB"
                     /locus_tag="ACG8MY_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0430138.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4275..4642
                     /gene="rnpB"
                     /locus_tag="ACG8MY_000918"
     ncRNA           4275..4642
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG8MY_000918"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4851..6017
                     /locus_tag="ACG8MY_000919"
     CDS             4851..6017
                     /locus_tag="ACG8MY_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0430139.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6063..7559
                     /locus_tag="ACG8MY_000920"
     CDS             6063..7559
                     /locus_tag="ACG8MY_000920"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0430140.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7640..8323
                     /locus_tag="ACG8MY_000921"
     CDS             7640..8323
                     /locus_tag="ACG8MY_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0430141.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8968..10158
                     /locus_tag="ACG8MY_000922"
     CDS             8968..10158
                     /locus_tag="ACG8MY_000922"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0430142.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10260..11213
                     /locus_tag="ACG8MY_000923"
     CDS             10260..11213
                     /locus_tag="ACG8MY_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0430143.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11282..12631
                     /locus_tag="ACG8MY_000924"
     CDS             11282..12631
                     /locus_tag="ACG8MY_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0430144.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12814..13137)
                     /locus_tag="ACG8MY_000925"
     CDS             complement(12814..13137)
                     /locus_tag="ACG8MY_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430145.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13266..14429
                     /locus_tag="ACG8MY_000926"
     CDS             13266..14429
                     /locus_tag="ACG8MY_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0430146.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14499..15848
                     /locus_tag="ACG8MY_000927"
     CDS             14499..15848
                     /locus_tag="ACG8MY_000927"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0430147.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15893..16324)
                     /locus_tag="ACG8MY_000928"
     CDS             complement(15893..16324)
                     /locus_tag="ACG8MY_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0430148.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16712..19354
                     /gene="alaS"
                     /locus_tag="ACG8MY_000929"
     CDS             16712..19354
                     /gene="alaS"
                     /locus_tag="ACG8MY_000929"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0430149.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19554..20150
                     /locus_tag="ACG8MY_000930"
     CDS             19554..20150
                     /locus_tag="ACG8MY_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0430150.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20272..>21335
                     /locus_tag="ACG8MY_000931"
     CDS             20272..>21335
                     /locus_tag="ACG8MY_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0430151.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTEL"
BASE COUNT         6877 a         3777 c         4517 g         6164 t
ORIGIN      
        1 tatatgctgg atagtcttat aaaaaatcat gtaatacagc ttggtacatg atttttattt
       61 gtagctgttt tttacataga cacaaaaaag aaaccacgct tgtagacgtg gtttcttttt
      121 tactatctgt ttccagaagc tgaggtgctg ctggatggtg tatggtcatt tgcagcatcc
      181 gctccgctag ttgctggctc actggaagaa gcaggaggag cagatgaaga cggtgtcgag
      241 ctttcttcgg atgaagaaga aggaaccgtt gttgaagacg ctggctcgct tgtttctgct
      301 gtcgtgcttt cttttgagga ctcagcagtt gattcgcttt gactagtaga gctagagctt
      361 tgagtcgttg tctctgttgt tgagcttcca ggagtctgga catagctgga agatggaata
      421 gttgttgatg gtgtaattgc attcgatcta gctgtatatt gatctttgta gtacagttca
      481 ccgcccacac gaattaatcc gcttggcatc tcccagtctg tatttgtcac gttagctgaa
      541 acatactgca taagctcgcg ataaacatca gaggctacgt gtgacgattc tgaagtgaca
      601 ggtgtcattt ttttattgta acctgtccac acggaaatcg aataatttgg cgtatagccc
      661 gcaaatagga tatctggata caccccagaa gaaataccaa gttttgcata ctcgtcatct
      721 gtgtaattgg atgttccggt cttaccggct tgatataacc cagcgatttg cgcattcgta
      781 ccagctcctt cagtgatcgt atcttttaaa atgtctgtga tcatataagc tgtttctgga
      841 gacatggctg tttttccatc tggctcataa gtttcttctg ttccgtcttg gaagacgatt
      901 ttattaacat attgaggttt gtaataagtc cctccattgg cgaaagccgc ataggctgct
      961 gccattttta aggaagaagc tccgtatttt gtcccatctt gttcttccgt attactagag
     1021 atcgcattcg attgatggat cgtactgtat tcgatcccaa gattttttaa gaaagaagcc
     1081 actttatctg aaccgacttc attgaataat ttgactgctg gaacattacg ggataagtaa
     1141 agtgcttggc gtaaagtaat ggtgcccata tattgattat cccagtttcc tacaggagtc
     1201 gaagtccctt catagttata cggtgcgtca gtaatagttt ttccagtaga atatttcaaa
     1261 tattcaaaag ctggtccgta atctgtgaca ggtttcatcg tagaaccaaa atcacgggaa
     1321 gtattcactg ctaagttgtt gcctaaagtt acgtcttctg cgatgtgacg tccgccaatc
     1381 tgggctttta ctttcccggt attggtgtcg ataagggtgg aggcaacttg catctcgtcg
     1441 tctgggtaag agacatactg atccgtgttg acgatatcat atagtttctt ttgggcgtcc
     1501 agatccaaat tcgtataaat ctctaaaccg tctgtgtaga cattcttatc tgttttttct
     1561 tgtacttcat tgatgacttc tttgacataa ttatcgacga tcttagtatt gtcatcggat
     1621 tgagttagtt cttgcagacc atccgttacc ggtacattaa cagcttgatc atattctgtt
     1681 tgagagattt tttcgttttg aagcatcgta tacaaaacag tatctcgtct tttcttcgcc
     1741 tgatctggat aaacataagg atcataggcg gaaggggctt gaggcatccc agcaagcaat
     1801 gctgtttgcg gtaaagaaag ttctgataat ttcttaccaa agtacatctc agaagctgtc
     1861 tccatcccgt acaagccgtt tgacatatac actttattta cgtagtaggt caagatctct
     1921 tgtttcgatt ttttttgttc caatcttaca gccatccacg cttcttgggc cttgcgtttc
     1981 agagtctgat cctcggcact ggtggagaag aaggaaagtt tgatcaattg ctgtgtcaaa
     2041 gtacttccgc cttgcagtcc gccagaggta aagttagaga gggcagaacc gatgatccga
     2101 atagggtcaa ccccgatgtg tttataaaaa cggcgatctt ctaccgacac gatcgcatct
     2161 tctaatgttt tcggtaattc attcgcagaa attttttcac gcttttctgc gcctaaatct
     2221 tcgaatagtt cgccatcttg tgtatatagt ttggaagata cggtagcgtc taatttttta
     2281 tccgtcaatt cgggcgcatc ttttgcgtaa taccagaaaa gtcctacacc tgctaaaaag
     2341 aggatacaga aaaatgagag tattcctaaa aggatcttga taaataatcc ttttttatgt
     2401 gttccagagg actttccaga acctttgcca gaattcttct tcacactctt tttaggagta
     2461 ggctgtttct gtcgtctaga gcttctagtt tgttcatttg ccattcttgc tttctccttc
     2521 aatagataat gttgataaat agaagtcaac cgcgtccaaa taaggaatcc gcggagaaat
     2581 accagttttg atctctattc ctagttcttc gatcaaagat aaaggtagtg atttgcgtcc
     2641 tttttccttt tgggcgtccc agtaagcaat caaatgaaca ctattcaaat aaaaacagcg
     2701 cttcaaagac gaaaaccata acaagacaaa gcagacccca ttttgttcga ggcatgcttt
     2761 catatgttcg atttgatggg catggaagtt tttaaaggga aaagatgttt tgttcttggt
     2821 ttcttttgct tcaaagtcca agtaaaagcc tcgatagacg ccattatagt ctgtagttga
     2881 ggcttgggta aagtaggctt ccttgatcac agcagcactt ctcttaggat agtctacttt
     2941 cacgatttgg ataggggtag gctttttatg gataaccgcc aacccgcggg aaagataata
     3001 tttattactt tcattgattg cttcttcaaa gcgcatcccc cgattgccga attcagtgat
     3061 ctgatttctt ttttcttgaa ttttttcgac gggagaggca ttcttataat aaggttggcc
     3121 atttggatag cgcaatacca tctttaatca cactcctagg ccacattata gcaaaaaagc
     3181 ggatagaaag aaaaggaatc tgagatggat aagtcaaaag taatctatgt gtctgggtat
     3241 cgaagtttcg aactcggtgt ttttaaagaa aatgatccta aaatccaagt tgttaaaaat
     3301 gttttaaaaa aagaactcca tcagttagca gaagaagggc tggaatgggt acttgtcagt
     3361 gggaatttgg gcgtcgaact atgggctgtg caaatagtag cggaattgaa agaagaatat
     3421 ccggaactat ctcttggtat tttgtatcct ttttctgatt ttggtaataa ctggaatgaa
     3481 gggaaccaag aaaaaaaaca attagcagaa caattagcag attatgtcga agctgttagc
     3541 catcaaccct accaatcacc tagtcagcta aagaatcaca ctcgttttat acttgagcat
     3601 acagtgggct gtctgctgat ctatgatgaa gaattccctg gaaaaacgaa gtattttttt
     3661 gaagatgcac agaattacca agcagatcat ccctacgaaa ttcggctgat cagtatggac
     3721 gatcttcaaa attttagtga ataatcgata ttgtgtttga tttatcaact tttttcctat
     3781 acaatgaaaa aggtagaaat gttattacgt aaatgaaaac tagaggtgta aatatggcga
     3841 atttggttta tagtcctaaa gacattttac aacaagaatt caaaacaaaa atgcgcggct
     3901 atgatcccgt tgaagttgat gaattcttag acaatatcat caaagattat gaaacttaca
     3961 gcaaagaact ccttgcttta caagaagaaa acgatcgatt gagtgcaaaa gtagctcaat
     4021 tatctaaaac tcaaggagca gctcaaacac gcgtgcaaca aacagaggca ccaaagagcg
     4081 cagcagtgac caattttgat atcttaaaac gcttgtcaaa tcttgaacgt gaagtatttg
     4141 gtaaaaagct tgatcaacaa gcaagtgcag tcaaaccagc acaacctaat ccaaataact
     4201 acacaaatgc agacacaagc ttagatgata atgaaaaaac acgtcaattt taatttttga
     4261 ttaatgaatt tgcggttttc gggtaatcgc ggtctgactt gatcaggctg aggaaagtcc
     4321 atgctcgcac aggctgtgat gcctgtagtg ttcgtgctta gcgaaaccat aagctaaggt
     4381 actctttgga gtaacggcag gaaaacaagc taaggcttcg gctatgctta agtatccttg
     4441 aaagtgccac agtgacgaag cggctgggga aacccatccg gtggaacgcg gtaaacccct
     4501 cgagcgagca acccaaacaa tggtaggggc gctttttgtc tggaagaaga acaggacaaa
     4561 aaggcagtat aactgcagat agatggttac cgctttgttt tttctccctg gaaaagcaaa
     4621 gtacagaaca tggcttatag aaaaccgcaa acatcaggga attaggtcgt gacaaaagta
     4681 atgcgttata agaaacaaga aaaacatttg aaagctattt ttcaatactt cagatgaatc
     4741 gttcttatta caaaacaaac ttttgtatca ccggttattc ggaatttgag taaaagtgtg
     4801 gggtaggact gatgtcccac ctcctttttt tataaataag gagattgtgt atggaaaaaa
     4861 acaggaagtt caacctcgtc gcaactgcgg caagcgggct agaagcactg gttggtaaag
     4921 aattaagaga tcttggaatc gaatgccaag ttgagaacgg acgtgcacgt tttgaaggaa
     4981 caatggaaac gatcgctaca gctaatttat ggttacgtac agcagatcga gtaaaaatcg
     5041 tggttgggga attcgatgcg tatacgtttg atgaattgtt tgaaaacgta aaagcattgc
     5101 cttgggaaga ttttcttcca ctagatgcag cttttcctgt agctggaaaa tcgatcaagt
     5161 cgaaactata tagcacacct gattgccaag cgatcacaaa aaaagcaatc gttgaacgtc
     5221 tccgtaccta ttatcaccgt ccagcttctg taccactgac ggaaactggc gcacattttc
     5281 agttggaagt agctttgctg aaagaccatg tgatggtaac tctggatacg acaggaccaa
     5341 gtttattcaa acgtggttat cgcttagaaa aaggtggagc accattgaaa gaaaacatgg
     5401 cagcagcact tattatgttg acaaattggc gaaaagaccg tccattttat gatcctgtat
     5461 gtggttcagg tacgctttgt attgaagcag ccttaatagg ccataatatc gcaccaggat
     5521 tcaatcgtga gtttgcttgc gaatcttggg attggttcag tcaagaaatc atggaaaatg
     5581 ttcgatcagc agccgaagaa aaagcagatt atgatattga attagatatt actggttcag
     5641 atatcaatgg acggatgatt gaaattgcca aagccaatgc agaagaaatc ggattaggct
     5701 cttcgattac ctttaagcaa caagcagtca aagacttcaa aacggataaa gaatacgggg
     5761 tcatcgtggc taatccgcct tacggagaac gtctaggaga agaagaaact gttcggaaat
     5821 tatataaaga aatgggcgat gtattccgtc cattaaaaac atggagtaaa tatatcttga
     5881 ctagcgactt agcgtttgag gaattttacg gacaaaaagc aaccaaaaaa agaaaattgt
     5941 acaatggtgc attgcgtacg gatctattcc agtattgggg aactcgtccg cctagaaaaa
     6001 aagagaaaga cgaataatct tactaattga ttcattcaat tggatgtttg gaggaatagg
     6061 gaatgaaaga aaaagagttt ttacaagaag taaaagaaat caatcttcta caacaagctt
     6121 taggaatcct cgattgggat acacaaacag gtatgcctga aaaagctagt gcataccgaa
     6181 gtgaagtaga cagctatctt tacagcattt atttttcaaa aaaaatcggt ccaaaaatca
     6241 aagaagccat tcgctatttt tctgaacatc cggaagaatt gtcggaagtt ggaactgccg
     6301 tttttgccaa agtaaaagaa gagtatgatt tggaacatcg cgtaccggaa gatcttatga
     6361 gaaaatatac agctgccgtt tcttcagcac acggacaatg gcaaaaagct agagaaacac
     6421 aacaattttc tgattttcaa cacgcattga atcaaaatat tgaattaaca aaacagttga
     6481 taccttactg gagaaaagat gaaaagacga attatgatgt tttgctgaat caatacgaac
     6541 ccgggatgac tgtcgaaatc cttgataagg tatttgatca gcttaaagaa ggaattcttt
     6601 cgatccgccg tacgctccaa gaaaaaggga ctgaacctga aacagacttt ttaaatcgga
     6661 gaatgacgaa aaaacaacag aagagatttg tagtgaaagt aatcgaacaa cttggttatg
     6721 attttagtcg tggtcgttta gatgacactg tccacccatt tatgactgga attaatccga
     6781 atgatgcacg aatcacgaca cgatggaacg aaacagattt caaaatggct gtttttggag
     6841 tgatccacga ggctggtcat ggtatgtatg aacaagacat cgatgataaa tatgcgtata
     6901 caccggttta tgaaggtaca tcaatgggta tccatgaatc acaatcgttg tttaacgaga
     6961 tcattattgg aagtagtcgc agcttctgga aaaagcaata tcctttcttt caagaatgtg
     7021 cagaaggaac attcgatgat attccttttg acaaatttta tcgttcattg aaccatacaa
     7081 aatcaagttt ggttcgtatc gaggcagaca gtttgactta ccctctccat atcatcattc
     7141 gttatgaaat cgaaaaaatg attttcaatg aaggggtaga tgttgctgaa ctacctgaaa
     7201 tatggaatca aaaatatgaa gagtatctgg gaatacgacc aacaaatgat gtggaaggca
     7261 tcctacaaga cgttcattgg tcaggtgcaa gctttggtta ttttccctct tatgcattag
     7321 ggtacatgta tgcggcacaa ttacttcatt ctttgcagca agaaatagat gtggaaaatg
     7381 ttctagcgtc agacgattat tctccaatca aaaactggat gtcagagcat atccatcaat
     7441 atggtgcatc tagaaaacca aatcaattaa tcatggatgc aacacatgaa ccgcttaatc
     7501 cgcaatacct gatcgactat atgaaagacc tgtattttaa tgtatatcag gtaaaataaa
     7561 atacaattta caaagatagc tgaaattcat ctaatctata gatgttttca gctatttact
     7621 taagaaagaa gtggaacgaa tggataaatt acaagtgatc gctgattata gtatggacca
     7681 attagcgcat gatcaaacag gacatgggag cgatcatacc aaaagagttg tcaagttagc
     7741 tgaaaaaatt ttggagacgg aaccgcaggc agaccgcttt gtgacgcttg cagcagctta
     7801 cctacatgat acgattgatg ataaagtcgt taaaaatgag aacgaggcaa agcaacagct
     7861 tcgtgtattc cttcgctctt tgccaatagt agaggaacag atctcgatga ttttttccat
     7921 tatcgaaaat atgtcctttt ctaaaaatct ctctgaagcg gccgaactat ctctggaagg
     7981 taagatcgtt caagacgctg atcgtatcga agcattagga gcaatcggca ttttgcgtac
     8041 tgcttatttc ggcggtggac atgggcaccc gatttttgat cctgaactgt atccgcaaac
     8101 attcaaggac aaagaaaatt accgaaaagg cacgacagtg atcaatcatt tttatgaaaa
     8161 actgttcttg ctgtctgata aaatgaatac tgattacgga tatgaagaag ccaaacgaag
     8221 agaaaccttc atgcgtgaat tcttagaaga attttttctg gaatggtcta ttgacgatga
     8281 aaattttact ccagacaatt ggatcaaaaa taacaaaaaa tgatggaggt tgtgacagaa
     8341 gtggtcagct ccaagatatt tcggatcatt ttccgtaaaa gttacttctg gaacactgtt
     8401 taccaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaat
     8461 agcttctcgt gtttgatgaa tattgtaact tgtgattcgg aattggcagg agaacaccgt
     8521 ttattcggaa gaataggact gtgacaagac ttttgtcaca gtcctttttt cgaattcaac
     8581 aagtaaaact tttaagaaat attcagagag taaaattaaa aattgctgat aaacgaagat
     8641 ggaaatttta tgtattttta ttggtctatg caataaataa attaaatatt cagaatacaa
     8701 ttgattttaa cgaaagggct tctatcaatg gttgtgttgt atttatctgc ccaggtagag
     8761 taaaatagat cattatctaa aaggtaattg ttcatactat ataagtcatt tgaaagtaaa
     8821 aagatactgt aaataaaaaa tgtggttaaa taattctcat aatcagtgta agcgttatca
     8881 aaaatatagc atacttgctt tgtagatgaa tgacacgata tatgtttggt atgttctgca
     8941 agacagatga atatggaggt ggagacaatg tcttccaaag aactaacaat gtatttagag
     9001 aaagaattgg atcaactgaa agaaaagggg ttatacaata cgatcgacat actagaaagt
     9061 gaaaatggtg cttgtatcat cgtagacgga aaaaagatga tcaatctagc atccaacaat
     9121 tatcttggat ttgccaatcg cgaagaattg aaaaaagcat gtattgaagc aaccgaaact
     9181 tatggtgtag gagcaggagc agtccgaacg atcaatggtt cgctgaaaat ccatcagcaa
     9241 ttagaagaaa aaatcgcaga attcaaaggg acagaagcag caatcgcctt tcaatctgga
     9301 ttcaactgca atatgggcgc tatctctgca gtaatgacaa aagaagatgc tatcttatcg
     9361 gatgaattaa accatgcatc tattattgac ggttgccgct tatccggagc aaaaatcatc
     9421 cgaattaaac atcaagatat gaaagacctt gagaaaaaag caaaggaagc gatagaaagt
     9481 aagaaatata aaaaaatcat gtatatcaca gatggtgttt tctccatgga tggagacatt
     9541 gcacgattgc cggaaattat tccgattgtt gaaaaatatg gactgatcac ctacgtcgat
     9601 gatgcgcacg gctcaggcgt aactggaaaa ggtgcaggaa ccgtcaagca ttttggatta
     9661 agcgacaaaa tcgatatgca aatgggcacg ttatcgaaag ctataggtgt tgttgggggt
     9721 tatgtagctg gatcgaaaac actaatcgac tggttgaaag ctagaagtcg gccattccta
     9781 ttttctactt cgctaactcc aggtgcagct gcagctgcat tggcttcgat tactttgatg
     9841 caagaacatc cggaacttgt cgaaaaagtt tgggaaaatg ctaattattt taaagaagaa
     9901 ttgaaaaaag taggctataa catcggtatg tctgaaacac ctattactcc agtcattttg
     9961 ggagacgaaa aagtgactca gactttctct aagaagctga tcgaacatgg gatttatgct
    10021 aaaccaatcg tttatccaac tgtgcctctt gggacaggca ggatcagaaa tatgccgact
    10081 gcggaacata cgagggaaat gcttgatgaa gcagtagcag tttatcaaaa aataggaaaa
    10141 gaaatggaaa ttatttaact aaatttagtt ttttcgtcta tttaaaatta gagaacatgt
    10201 tcacgcatat attgttaatc atctgaagat agtagtaaaa aagatcaggg aggatcatca
    10261 tgaaaaaagt attggtgaca ggatgtttag gacaaattgg ttcagagttg atcacacggc
    10321 tacgtcaaga actaggcagt gaaaatgtca ttgcgacaga tatacgcatg cctgaagtca
    10381 ttgagaaaaa cgatatcttt gagatcctag atgtaatgga ttacgaaaaa atgcgcagca
    10441 tagcagaaca atatcaagtg gacacattga ttcatttggc tgctcttttg tcagctgttg
    10501 cagaagcaaa accacaatta gcctggaatt tgaacgtgac tgggctggtc aatgcattgg
    10561 aagtcgctag agaattccag ctgaaatttt ttacgccaag ttcaatcggc gcgtttggtc
    10621 caaatactcc aaaagatcat accccacaag atactcttca gcgtcctaca accatgtatg
    10681 gggtaacgaa ggtagctgga gaactactat gtgattatta ttatgtgaaa tatggcgtag
    10741 atactcgtgg tgtccgtttc cctggattga tttcttatgc aaccttgcca ggaggcggta
    10801 cgacagacta cgcagtcgat atttattatt cagcaatcaa agaaggaaag tacactagtt
    10861 tcattgatca aggtacatca atggatatga tgtatatgcc agatgcgatc gatgccatta
    10921 taaaactcat gaatgcagat cctgatcgat tggttcatcg aaatgccttc aatatatcgg
    10981 cgatgtcttt tgacccggaa gagatcaaag catctattca aaaaatcata ccagattttg
    11041 aaatggatta tgatgtggat cctgtacgtc agtcaatagc agaatcttgg ccaaattcac
    11101 tagatgtttc ttgtgcgcaa gaggaatggg gcttttcgcc gaaatacgac ttagatgtga
    11161 tgacaaagga tatgttggaa aaactgaaag aaaaattggt agagacggta taaaacaaga
    11221 accacatcca aaaagttttt tatacttttg ggattggcta tggtatgaaa ggggacaagg
    11281 gatggaagaa tatcaagcca cgccagtaaa agaagtaaaa atagaaaatg aattaaaacg
    11341 tacgatggga ttttttactg ctttatcgac tgttatgggc acagtgatcg gtgcaggggt
    11401 attttttaaa gccgcaagcg tagcagaagt tacaggatcc gccagtttgc atatgttttc
    11461 ttggtttctc ggtggtatga tttctgtctg tgcaggacta acaggagctg aactggcagc
    11521 tgctattcca gaaacaggag ggatgattaa atatatcgaa cggatttacg gaaatacagc
    11581 agctttttta ttgggctggg cgcaagtcgt gatttatttt ccagcaaatg tagcagcctt
    11641 gtcgatcatt ttcggtacac agttcgtcaa tctatttggt ctttcccagt caatgattgt
    11701 tcccgtagct gttacagctg ctgtatcgat cctgttgatc aattttttag gttctaaagc
    11761 tggaggggct tttcagtcga ttacgcttgt ctgcaagctt attcctttgt ttgtgatcgt
    11821 cattttcggt ttgttccgac aagaaggagt agacttccaa ctgtttccga tccaagcagg
    11881 agaaaatctt tcatttttct cagccttggg agcgggcttg ctagcaacga tgttcgcata
    11941 cgacggttgg atacacgtag gaaatatttc gggagaattg aaaaagccgg cgaaagattt
    12001 gccaaaagca atttctttag gaatcatcgg aatcatgatc gtttatttat tagtaaatgc
    12061 ggtgttctta agaaccgcat ctatcgacgg agtcgcagga aacagcaatg cggcaagtga
    12121 cgttgcaaaa atgatttttg gcggcttcgg tggaagactg gtcacagttg gtatcttgat
    12181 ttctgtttat ggaaccatca atggctatac ccttactgga atgcgtctcc cttatgttat
    12241 tgcaaaagaa aacaacctgc cttttagcaa gctctttgct aaacttcatg ataaaactaa
    12301 agttcctgta gccgcaggta tcttagagct agtcatcgct attggcatga tgatgatcgg
    12361 tggttttgat acactaacgg atatgctgat ctttgtcatt tggatttttt atacgatggt
    12421 ctttgttggt gtcattcttc ttcgtaaaaa agaacctgat ttgtttcgac catacaaagt
    12481 tccaatgtat ccgtttattc cactagtagc aatcattgga gggactttta tcgtatcaag
    12541 cacgcttatc actcagacct ttttagcttc gatggggata gtattgacac ttgctggtat
    12601 cccgatttat ctctatttaa aacgtagata gctaaaacat tcgaatttta ttagaactgc
    12661 acttctctgc caaacaaaat attttattta aatgaggagt acgtggaaga aatgtcaaaa
    12721 gtactcgtta attttgatca cgtaaatgtc gttttgtcta cgatgaaata gcaaagtaaa
    12781 agacagaaat cgggctcaac cgatttctgt cttttacttt gatttgcgta actgaactat
    12841 tcggcgtacg ccgcgataaa tatttaataa aaccaagcaa ccagaaatga aaatgggaag
    12901 ccaaatcaga tagacagggt agccaagcac tttagacaaa gcatgtggag tcattttatc
    12961 catttcaaga agtgcttgct gccaagtctg aagattgaag actagcatga aacccgaaat
    13021 aatcaggaga ataaatccta aataatgaat tgtagttctt tttcttacat aagcgatcgt
    13081 agcataaagg aacagtaaaa aaataggtac gccaaaaaca aagtacatat tcagcatctc
    13141 gacaccccct ttgtgtttat cttactttac tttgaaagaa atgagaaata atagcctcgt
    13201 tacttgcttg aaacagcaaa ggactgctaa gataagagag atacaaagaa tcaaggagga
    13261 gtcatatggc aagttatatc gaaccgattc gttttgcgct gattgttttt ccatttttag
    13321 ccttagcgat ctcatctgtt ttttttattt atgaatatcg aaagtatgga acatttctag
    13381 taacaagggc agttattttg tattctttcg ttttttattt attgtgtgcg tattttttgg
    13441 tgatcttacc acttccggca aaggagacgg ttgcccaaat gacagggcca aagatggagc
    13501 tgcatttatt tgcttcatgg caacatttca gagacaacac ggtattggtc ttgtctgaac
    13561 ctagtaccta cctgccggca atgaagcaga gtgtattttt ggaaccagct tttaatgttt
    13621 tattactcat accatttggc atttatttaa gatattattt ccggctttct tttatgaaaa
    13681 cgatgattgc tagtttctgc ctttctctat tttttgaatt gacgcagcta agtggacttt
    13741 attttatcta cccaagacca taccgcttat ttgatgtgaa tgatttagtg acaaatacat
    13801 tgggtggaat cattggtttt gtcgttacgc cgttatttac atttatgctt cctacaagaa
    13861 gacgaatgga tgaactttct tatgaaaaag gaaagactgt ctcattgact cgacgcttgg
    13921 tcgcttattt gatcgactgg acatttttat ctcttattaa tttggtagcg gcgattttct
    13981 ttaaaattgc gttcgattca ggggcagtta cttctcatat ctggtggctt ttattagaag
    14041 taggggtata ctttatgatt gtaccgttta taaccaatgg atggacactt ggcaaaaaaa
    14101 ttgtgcgtat ccgtttggtg gaagaagacc gcgaacgaat ttctttaaaa gcattgatga
    14161 tccgctacgg gtatctgtac tttctttttt atggtttaag ctggttgggg cagttgcttc
    14221 agcaaatcat gaattcgaca acgaatacgg tgttgcagat gagtgcagta ttcatttctc
    14281 tcggtgtctt gttactacaa ggtattttta ttttaaatat cttgctatca tttttgagaa
    14341 aaaaacggat tctgttttat gaaaaagcta gtcacacata tactgttagc acgattggcc
    14401 aaacaaagga tgatgattct cgaaattagc tgagcaaaaa cgctttgtct gtgttataat
    14461 gccaaagtcg tgataattta aagagaagag gtgcacgaat gaataatttt gatatattcc
    14521 gcttcaaacc gtttatcaat gaagcgttaa ccgcaaaggg cttcaaacag cccacagagg
    14581 tccaggaacg tttgattcct ttaattgcaa aaggaaaaaa tatcattggg caatctcaaa
    14641 ctggtacagg aaaaacacat acgtttttgt tgcctttgtt tgaaaaaatc aatgcagaaa
    14701 aagaggaagt acaagttgtt attacagctc caagtaggga actagctgca cagatctatg
    14761 aagcagctac acaaatcgct aaattttcta caccggagat ccgtgtctcg aattttgtcg
    14821 gcggtacaga caaacagcgt caattaaatc gtctgcatca ccaacagcca caggttgtag
    14881 taggcacgcc tggtagaata ttagacatga tgaatgaaca agcgctgaag attcacaccg
    14941 catttgcatt tgttgtcgat gaagcggata tgacattgga tatgggtttt ttaaatgaag
    15001 tagaccaaat tgcaagtcga ttaccagaaa agctgcagtt tttagttttt tctgccacta
    15061 ttccagaaaa acttcgtcca ttcttacgaa aatacatgga aaatccaatc attgaagaaa
    15121 tcaaaccaaa agcagttatt tctgaagaca ttgaaaattg gctgatttca acaaaaggta
    15181 aagatgagaa tcaaatgatt tatcaactgc tgacaattgg tcatccttat ttagcaatcg
    15241 tatttgccaa tacaaaacag catgtggatg aaattgccga ttacttgaaa aaccaaggct
    15301 tgaaagtagc aaagattcat ggagacatca ccccaagaga gagaaaacga gtgatgcgac
    15361 aagtacagaa tctggagtat caatatgttg tagcgactga tttagctgct agagggatcg
    15421 atatcgaagg tgtctctcat gttattaatg cagaaattcc agaagatcta gacttcttta
    15481 tccatcgtgt cggacgtact ggacgtaatg gcttaaaagg aacggccatt acgttatatt
    15541 cgccaaatga tgagcaagcc attgatgcga tagaaaaatt gggcgtctca tttgaaccaa
    15601 aagaaatcaa aaacggggaa atcgtcaaaa catacgatcg taatagacgt gccaaacgag
    15661 aaaaatcaaa agatgtatta gatcccacgc tgattggtct tgtgaagaag aaaaagaaaa
    15721 agatcaagcc tggttataag aaaaaaatcg attgggcaat tgcagaaaaa aataaaaaag
    15781 aacgcaaaat cgaacgcaga cagcaaagcc gtactgctcg taaaaataag aaaaattcac
    15841 ataaataaaa aacaagaggt tgtgacaaaa ggcgtgtcac aatctcttgt ttttaagcta
    15901 gacttttttt cattccgata caaagtactt catcttcaat aaaaggttct gtgcaagtat
    15961 gatacccttg tttttcataa aaatctacag ccgtcatttc agctactaca taactagtct
    16021 gacaaccttc tgaaaccgcc cgtttttcag cagcttcaag caactgaccg cctaaccctt
    16081 ttcctcggta ttcttttgct acgcaaaatc gttccggctt gaagcaagta ggtgttttct
    16141 tttcataacg caaagtagct actggtcttt tttgatcaag aaatagataa taggtttgtt
    16201 gcgggatatc ttttccgtcg aattctagtt ctgcaggaat cccttgttct aatacaaaaa
    16261 cttgatatct caaaaagcag ctagctgcgc gtatccattc atccgctcca actatttttt
    16321 tcatcatatt cctccttgtc atgaatagca gagatgtatt gattccttta ttcttatcgt
    16381 accaaaaatc gtggagtatg accaaaagga aatgaaaata aaaattgaga tattgacagt
    16441 gatcaataga atgcgtatac taattaagta cgaaaaggat atgatttgtt ttttaagact
    16501 cacagaaaat tctttcttgg ctgaaaaaag aatagtcact aatcaaattg ctcccttttt
    16561 ctattttggt agaaataccg acgcagctca gcgttatcga gtttaagagg taatgattac
    16621 acatcattgc aatcaaggtg gtaccgcgag ttttcgtcct tggctgtgat ggtgtttttt
    16681 tgtatttaat ttatagaaag caggtaatga catgaaacaa ttaagtagtg cacaagttcg
    16741 acaaatgttt cttgatttct tccaatctaa aggacatacg attgaaccta gtgcgtcttt
    16801 gatccccgtg aacgatccaa ccttattgtg gatcaactct ggtgttgcaa cattgaaaaa
    16861 atattttgat ggttcagttg tgccagaaaa tccaagaatc acaaatgccc aaaaatctat
    16921 tcgaacaaat gatatcgaaa atgtcggtaa aacagcacgg caccatacga tgtttgagat
    16981 gctgggtaat ttttcgattg gtgactattt caaaaatgaa gccatccact gggcatggga
    17041 atttttgact gatccaaaat ggatggcatt cgatccagaa aaactttatg taacagttta
    17101 tccaaaagat acagaagcaa aacgcatctg gcatgaagaa gttggcctgc cactagatca
    17161 cattattgat gtagaagaca acttctggga tatcggtgca ggcccatgtg gtccggatac
    17221 ggaaattttc tatgaccgtg gagaagctta taacgattta gcagaagacg atcctgaaaa
    17281 ctatccagga ggagaaaatg aacgttattt agaaatctgg aacttagtat tttcagaatt
    17341 caaccataag cctgataaca catttgaacc acttcctcat aaaaacattg atacggggat
    17401 gggattggaa cgcatggtct caatcgttca agatgcgtca acgaactttg agacagactt
    17461 gtttatgcct attatccacg ccgtagaaaa gctaagcaaa caagtaaaat atggtgaaaa
    17521 cccagtaaca gatatttctt acaaagtaat cgcagatcat attcgcgccc tttcatttgc
    17581 tattggcgac ggtgcattgc catcgaatga agggagaggg tatgtcttac gccgtttgct
    17641 tcgtcgtgct gtcatgcatg ggaaaaaatt aggtattgaa caagcattct tatatcgttt
    17701 agtccctgtt gttggggaaa tcatggtaag ttattatcct gaagtacttc aacaaaaaga
    17761 atttattgaa aaagttatcc gtacagaaga agaacgtttc aatgaaacaa tcaatgaagg
    17821 tttggaaatc ttaaatcaag tgatccaaga agtaaaaaac gaaaacggga cgactctagc
    17881 aggaaaagac atcttcaaac tatacgacac atatggcttc ccggtagaat taacggaaga
    17941 ggtggcagaa gatgaaggac tgaaagttga tcatgctggt tttgaaaaag aaatggatgc
    18001 acaacgcgaa cgtgctcgct ctgcccgcag tactgaaaaa tcaatggggg tacaatctgc
    18061 tttattgaca gatatcaaag tagaaagtaa attcattgga tacacagaac aagaatcaga
    18121 tagtcaatta ttggtcatcg ttaaaggtga gtctcttttg aatgaagtcg cagaaggtga
    18181 agctcaaatg atctttgatc aaacaccttt ctatgcagaa atgggtgggc aagtagcgga
    18241 tcaaggatgg atcatgaatg aagtaggcga aatcgtagca gttgttacag atgttcaaaa
    18301 agcgcctaat ggtcaattta tgcacaccgt agaagtaaaa gcaccaatga gtgaaggaca
    18361 aacctatcat ctattgatcg atcaatcgat gcgcaatcgg atcatcaaaa accatacagc
    18421 aacccatcta ctgcacaagg cattgaaaga agtacttggc gagcatgcaa atcaagctgg
    18481 ttctttagtc gctcctggac atttacgatt cgactttacc cactttggtc aaatcacttc
    18541 agatgaatta gctgaaatgg aaagaatcgt caacgaaaaa atctgggaag ctatcccagt
    18601 tgaaacaatt gaaacagata tcgatacagc caaaaacatg ggtgcaatgg ctttgtttgg
    18661 tgaaaaatac ggacatgacg ttcgagtagt aaatattggc ggctggtcaa tcgaactgtg
    18721 cggaggcaca catgtagcaa atacagaaaa catcggaatc ttcaaaatcg tttctgaatc
    18781 aggaattggt gcaggcgtgc gacgaatcga ggctgtaaca agtaaagaag cgtacgaact
    18841 acttgaagaa gaagaaaaac aactaaaaca agtagcaaca attgttaaat cacctcaatt
    18901 gaaagaagtt gtttcaaaag cggaacaatt gcaacagcaa gtaagagagc tacaaaagga
    18961 aaatgaacaa ttagctagca agttagcgaa tcaacaagct ggtgatgtat tcaaagatcc
    19021 agaaacgatc aatggaaaaa caatcattgc agcacaagta aaagtgaaag atatgaatca
    19081 gctgcgtcaa ttagctgacc aatggaaaca aaaaaatatt tcagatattc ttgttttagc
    19141 aaacgaacaa gaaggaaaag tcagtctgct agcggcaatg acgaaagaat caaatgaagc
    19201 tggactaaaa gctggcgact tgattaaagc aatcgcaccg aaagtaggcg gtggcggtgg
    19261 tggacgtcca gacatggcac aagctggcgg gaaaaatcct gctggtattc cagatgcact
    19321 taaagctgcg aaagaatggg ttgcgcaaca atagggcgtt tacgtttgac agaaaaacaa
    19381 aagttcaaac attgaacgaa acatgattat gggggcgaag acaaacaagt cttgccctca
    19441 tacttttttc aattggatgg cgcatctaat caaatggtcg gattcggatg gtctttagtt
    19501 gacgaaagtg acagaaattt ttacgattag tataaataaa aaggggtgaa aaaatgaata
    19561 gtagaaataa gacctttcga ctagtcttac gtgcgatctt gttagcaatc atcatcgtac
    19621 aagcgatggt tccttggctg gggtttattc cattaggatt cattagttta acaattatcc
    19681 atatcacagt aattattgct gcagtagtat taggcccaaa agatggcatg gtgattggct
    19741 tgttttgggg aatcgctaca attgttcgtg cctatgccat gccaacaacg ccatttgata
    19801 cattagtgtt cacgaatccg attatctcgg tagttcccag agtacttgtt ggcttagtag
    19861 caggcttggt tttccactgg atttatcaaa aaaataaatc aataacgatc agctcagcct
    19921 ttgcaggggt actgggttca ttggtcaata cagtcttagt gttaggattc atgggattat
    19981 tttatacagg tgcaacggca gatgcctatg gtgtagactc tgctctttta tttaaaacct
    20041 tagcaggggt tgcagcaatc aatgggatac cagaagcaat aggtgcggga atcataacac
    20101 cattactagc aaaagcgtta tttgcagcaa caccgttgaa accagaataa tgaccatctt
    20161 ttaatagtaa taggacgcac gatagataga aaaaggaaga gaggagagga aaacaatcgt
    20221 cctctccatc tttttacctg agcaacaagc aaactagaag gggtaaaaaa cttggctaag
    20281 aaacaagaag aaaaaaagaa acaaataccg caacagcgga caaaaaaagg agtactagcg
    20341 ggtatcttag gattggttac gaatctgatc ttgtttgtct ctaagctaat gatcgggctt
    20401 ttttcaggaa gtgtctccat tatggctgat gcagtgaaca gtctatcaga tactgcttca
    20461 tccatattga ctttgatcgg atttaaaatt gctgcaaagc cagcagatca ggaacatcct
    20521 tatggacacg aacgcttcga atatatcagt gggttatttg tttcgatcat cattacttat
    20581 gtaggatttc aatttttaga gtcttctgct gaaaaaatca tccatccaga aaatgtctct
    20641 ttaacaccca tcgtcttttt tgtcctaatc atttccatct ttttgaaatt tgcacaaggc
    20701 aaaatgtatc aggttattgc gcaatccatt gattctgaaa cattgagagc tacagcaaca
    20761 gatagttata atgatgtctt tactacactg gctgtattgc tttcagcagc aatcgaatgg
    20821 gtaactggat ggcgtattga tggatatata ggttttgctt tagcattata tatcctttat
    20881 agtggattga cgatgcttcg aaattttgta tacgaactat taggaagtcg cccaacgcca
    20941 gcagagattg aagaaatgga agagcattta aatgcttgcg atgatatact tggctatcat
    21001 gatttattgg tgcataacta tggacccaat aaacgctttg catcagtcca catcgaagta
    21061 gatgaatcaa tgaatttgaa tgtggctcat aagattattg atctgataga aaaagacttt
    21121 aaaaagtctt tggatgtgga acttgtctgt cacttggatc caatgcctgt tgataacgaa
    21181 aaatactata atatcttgcg gcagttaaag caaattcttc ttgaaacagg tacagggctt
    21241 aatattcatg attttagagt aattcatcaa ggaaaaacgc tgcaatttga tgtagtggtc
    21301 cctgagaaat tccagtttgt tgatacagaa ctaga
//
LOCUS       DAZKNO010000034        20936 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000034 DAZKNO010000000
VERSION     DAZKNO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.034"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..705
                     /locus_tag="ACG8MY_000932"
     CDS             4..705
                     /locus_tag="ACG8MY_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0430152.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1277..2293)
                     /gene="tsaD"
                     /locus_tag="ACG8MY_000933"
     CDS             complement(1277..2293)
                     /gene="tsaD"
                     /locus_tag="ACG8MY_000933"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0430153.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2290..2754)
                     /gene="rimI"
                     /locus_tag="ACG8MY_000934"
     CDS             complement(2290..2754)
                     /gene="rimI"
                     /locus_tag="ACG8MY_000934"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0430154.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2761..3303)
                     /gene="rimI"
                     /locus_tag="ACG8MY_000935"
     CDS             complement(2761..3303)
                     /gene="rimI"
                     /locus_tag="ACG8MY_000935"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0430155.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3287..4009)
                     /gene="tsaB"
                     /locus_tag="ACG8MY_000936"
     CDS             complement(3287..4009)
                     /gene="tsaB"
                     /locus_tag="ACG8MY_000936"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0430156.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4200..5180)
                     /locus_tag="ACG8MY_000937"
     CDS             complement(4200..5180)
                     /locus_tag="ACG8MY_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0430157.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5204..6688)
                     /gene="galT"
                     /locus_tag="ACG8MY_000938"
     CDS             complement(5204..6688)
                     /gene="galT"
                     /locus_tag="ACG8MY_000938"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0430158.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6700..7689)
                     /gene="galE"
                     /locus_tag="ACG8MY_000939"
     CDS             complement(6700..7689)
                     /gene="galE"
                     /locus_tag="ACG8MY_000939"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0430159.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7938..8105
                     /locus_tag="ACG8MY_000940"
     CDS             7938..8105
                     /locus_tag="ACG8MY_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0430160.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8167..9978)
                     /locus_tag="ACG8MY_000941"
     CDS             complement(8167..9978)
                     /locus_tag="ACG8MY_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0430161.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(9975..10340)
                     /locus_tag="ACG8MY_000942"
     CDS             complement(9975..10340)
                     /locus_tag="ACG8MY_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0430162.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10503..10898)
                     /locus_tag="ACG8MY_000943"
     CDS             complement(10503..10898)
                     /locus_tag="ACG8MY_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0430163.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10916..11878)
                     /locus_tag="ACG8MY_000944"
     CDS             complement(10916..11878)
                     /locus_tag="ACG8MY_000944"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0430164.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11878..12090)
                     /locus_tag="ACG8MY_000945"
     CDS             complement(11878..12090)
                     /locus_tag="ACG8MY_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0430165.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12111..12809)
                     /locus_tag="ACG8MY_000946"
     CDS             complement(12111..12809)
                     /locus_tag="ACG8MY_000946"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0430166.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12829..13371)
                     /locus_tag="ACG8MY_000947"
     CDS             complement(12829..13371)
                     /locus_tag="ACG8MY_000947"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0430167.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13503..14507)
                     /locus_tag="ACG8MY_000948"
     CDS             complement(13503..14507)
                     /locus_tag="ACG8MY_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0430168.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14504..15493)
                     /locus_tag="ACG8MY_000949"
     CDS             complement(14504..15493)
                     /locus_tag="ACG8MY_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0430169.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15490..16296)
                     /locus_tag="ACG8MY_000950"
     CDS             complement(15490..16296)
                     /locus_tag="ACG8MY_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430170.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16462..17418
                     /locus_tag="ACG8MY_000951"
     CDS             16462..17418
                     /locus_tag="ACG8MY_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0430171.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17495..18013)
                     /locus_tag="ACG8MY_000952"
     CDS             complement(17495..18013)
                     /locus_tag="ACG8MY_000952"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0430172.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18101..18250)
                     /gene="rpmG"
                     /locus_tag="ACG8MY_000953"
     CDS             complement(18101..18250)
                     /gene="rpmG"
                     /locus_tag="ACG8MY_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0430173.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18478..18924
                     /locus_tag="ACG8MY_000954"
     CDS             18478..18924
                     /locus_tag="ACG8MY_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0430174.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19119..>20936)
                     /gene="asnB"
                     /locus_tag="ACG8MY_000955"
     CDS             complement(19119..>20936)
                     /gene="asnB"
                     /locus_tag="ACG8MY_000955"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0430175.1"
                     /translation="HRGPNSSGEYIDKHVALGFRRLSIIDLEGGTQPIYNEDRTKIII
                     FNGEIYNYQPLREELIAAGHVFQTHADTEVLLHGYEEWGTELLQKIRGMFAFAIWDNE
                     KNELFGARDHFGIKPYYYAEMNGTFMFGSEIKSFLPHPDFNKELNKEALKPYMTFQYS
                     PLNGETFFKDVYRLPEGHYYTYKDGKLDIQQYWDADFETKETHSRQEWIEKIDETVQA
                     SIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPDHSFSIGFDDKTYNEAIEARKLTELLD
                     LDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPSCVPLYFLANLASQSVRVVQSGEGADE
                     LFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTRYNLGRKIGKMKNFHGRIHLYESLAPA
                     KEYFIGHARVFEESEASEVLTPKYQTAPSVDEIMTVHYEKTEGIKDEVNKMQYVDLHQ
                     WMPKDILLKADKLSMASSLEVRVPLLDIEVMKLAQQIPSKFLLNQNNTKDIFRQAANK
                     HLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVKALFEADFAKEFFDQEKIMKLLDDHHE
                     GRKEEQRKIWTIFSFLTWYKVYFVDETIPQAEAIDYVTV"
BASE COUNT         6268 a         4475 c         3638 g         6555 t
ORIGIN      
        1 ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac tcagatatca acctgatttt
       61 gaacatttct cggatgaaga gttccaacag atcaaggaac atactgttct tcgttcttat
      121 aaaaaaggac agatcctttt cgacgatggt gatcccagac aacggttgta tatcttgacc
      181 aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa cttattacta ctatgactat
      241 gtgaatacaa ataacctttt tcctatgtca ggaatgtttg gagaagagac ctacacttat
      301 acagctcaag cgatgactga tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa
      361 ttagcacgaa aaaatcccga acagttgatc tatatgatac aaaaaatttc taaagtagtt
      421 gaaatacatg aattgcgtat ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa
      481 caaaatttat tcattttgaa agaagaattg ggcgtaccaa caaaagatgg tcatatcacc
      541 attccttatc cgatcacctt aaaagaatta gcagtgaata gcggcacgac acgtgaaact
      601 gctggacaag taatcaaaca actacgtgat tccggactgt tagattatga caaaaagcgt
      661 tttactttct gtaagacagc tactactcca gctatgagag aataacgcga acaaaaataa
      721 gcagtgaccc tgatatttat cagggtcact gcttattttc ctccctaaaa cttctgaaag
      781 atccggcagt tttagatcac tgggcaagac tcaaagattt tctatgtgtg tttcagttat
      841 gtaatattca ctgtttgcaa aaaatactga tagattaact agaggaagcg tcttgtcctc
      901 atccgaccta gattattcga tattcctcga caataaacga ttaatcagtg agggcaatca
      961 gaggaacgga taatgatgaa taagctgtaa aaaatagggc tttgacaaaa gcgtgtcaca
     1021 gcccttattt cgattaaacg gtgtgtacct gtcaactctt cggtcacaag tcataatgtt
     1081 cataaacatg aggagctgtt ttttgaaatt gttccttgtg aatcagagtt aaacgacagg
     1141 cttacgaact cttgattaaa cggcgttcaa aagcttgcat acgcggtaat aagaacagcc
     1201 aggtcaaaaa catgagaagc cgtttttcct gggctgttct tattacttga tgcagggcag
     1261 atttttcaca acctctttat tcagataaaa ccaagcttgg atcagcattc aactgatagc
     1321 tagcaaaatt tcttttttgc atttccacat gtgctgctgc tccaatcatt gcagcattat
     1381 ctccacaaag tcgaagaggg ggaatcacta gttctacctc agggagttta gttgccaaag
     1441 catcctgcaa tccttcacgc aacccttgat tggccgctac acctccagct acaatcagtt
     1501 gcttgactgg ataattttga caagctcgaa gtgttttagt aatcaataca tcgatcacac
     1561 ttgcttgaaa gctagctgct aaatcatttt tgtctagatc ttctccacgc tgttgtgcat
     1621 tatgcaccaa attgataaat gcacttttta atccgctaaa actgaaatca taattatctt
     1681 catggatcat cgctcgtgga aaatggtaat tatcttttcc ctgatgagct agctgatcga
     1741 tttcttttcc gcttgggtaa gaaagaccta atactcttcc aactttgtca taagcttctc
     1801 ctgctgcgtc atctcttgtc tccccaatga tttcatatga tccatcttct tgcatataga
     1861 ctaattctgt atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa
     1921 ccagacgggc tgcatagata tgccctgcca tatggtttac aggaataagc ggcaattggt
     1981 gtgcccaagc aaaagcctta gctgcagaaa taccaatcag caacgagccg acaagtcctg
     2041 gtccataagt gacagcgact gccgataaat cttctgccga cacaccagct tctaccagtg
     2101 catcttccaa gcataaggtg atttgttcta cgtgatggcg gctagctact tcaggaacta
     2161 cgccaccaaa acgtttatga ctgtttattt gagaagcaac gatgttagat aagatctctg
     2221 ttccatttct aacgaccgct acactcgttt catcacaact tgattcaatc gccagaatca
     2281 attcttcatt catttcatcg tctccgtttt taactttctg ctcataatga tcgcatcttc
     2341 cgtcggattg cgataatatg atttccgttt acctaatata cgaaactttt ctgattcata
     2401 aagttttctt gctgcttcat tagatagacg tacttctaaa aaaacatgca gcgaatcttt
     2461 tatttgttcc gtaatcagta attgtaacaa atgccgtgca tgcccttttc cttgttctga
     2521 aactgcaaca gcaatatttg tgatctcgac ttcatctatt actttcgagt atccaataaa
     2581 gccacagatc tggttatctt caactaaaag cagataatca gagtgaggct gttggatgtc
     2641 tgccaaaaac tgcattttcg tccaaggtga accatgtgga taagcatttt cgctaatcat
     2701 ccatacgcat tctgccaagt acgcttcatc aaaatctttt tttctgcaaa tcaactaatc
     2761 tcactcattt tctaaaagtt ttatcatatc caacgcatct tcttttgttt cagtgtaata
     2821 tctttttttg atcgttgttg cttgaaaacc taacttccga taaagtcttt gtgcatcttg
     2881 attactcata cgcacttcta atgacatcgt ctcacagcga ttcattatag caaagttctc
     2941 gatttcgtca ataagcagcg aaccgattcg tttacgctga tatgccggtc gaacggcaat
     3001 attcgtgata tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc
     3061 attatcaaag atacctagat atagatgagg aagcggtgac ttcaattctg atataaaggc
     3121 actttttgtc catggcaatt cgccattata gacttctcgt tcgacatcca gcagctcttt
     3181 gatatcttct gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt cgattgtttt
     3241 ttctgtataa atcattttat cttgaaaaaa gcttctggca tacgctctaa attttttcaa
     3301 cataactttc gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct tctacccgtt
     3361 ttaaatagcg aggaagaaaa gcttgtttct ctgcaactgg ttcagctaaa gcacctaatt
     3421 gagccacagt tacaccactt ggaatatccc atgctggaac tgtattaatc atcgcactag
     3481 gcaatatttc ctttatttca tagaagaatt tgtgacaatc actcccgacg aaataaatag
     3541 cttcattcag cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa
     3601 gtacaatttc taatctattt tctttccagc ggtatgcccc agcgtataca ttttttcttc
     3661 gagcatcaaa catgggcaca ataattttat ccacaccgat gcaattagcc gcgatggttt
     3721 tcagactaga tactcctact agctctttat ctaatgtata agcaatcgtt tttgctgtcg
     3781 ttacacctat acgaagccct gtataagaac cgggaccatc agaaaccgcg attcggtcaa
     3841 tgtcttttgg acttatttga agatctgcaa acaattggtc gattgcaggc atcagtgtta
     3901 cactgtggtt gcgtttgata tttgtttgta tttgtccaag aatttgttcg tcttctacca
     3961 caccgatcgc caaagtttgg tttgcagtat ctattcctaa tgtaatcata tatttgatcc
     4021 tttctgctat gacattctcg ccttccattg tagcatactt ttttattctg aggatgaaaa
     4081 taccaaaggc aaaactagta caatgataca tgattatctg ctggcgggca aattttgccc
     4141 cttcctgaaa cacaaaaaag ctgcggtatc acttcatttc cccacagctt ttccatcttt
     4201 tatttcgttg attctcgaat aatcaactct gtttccacaa taatctttct tgaaaccggt
     4261 gctttggaat tacttaattg aagaatcgtc tccactgcta gctctcccat ccattcggta
     4321 taaactttta cagtagaaag agagggcgtt acatatttcg ctacactgac atcattgaaa
     4381 cccatcacag aaatatcttc tggtaccact aaaccttttt cttgaatcgc tttcagtgcg
     4441 ccaatcgcta atgcatcatt agctgcaaaa agcgcatcag gaacatgcgg attcgttttt
     4501 aaatactgct tcactgcttg atatcctgac tcgacagaaa aatctgcttc gataattgga
     4561 ccttcgtcgt agcctttttc tgccaaaact tgttgaaatg ccataattct gggatctttt
     4621 agccgctgat ggtttttctt cgtatattct gttccagcaa gcatagcgat atgttgcgct
     4681 ttatgggaga caaaatgttc aatgactttt tgaacacttg acttaaagtc gacaactaga
     4741 gagtcgattc cttgagaagt gccgtcagaa tctaccaaaa gcaaggcatt ttgggatcct
     4801 ttcatttcta ataactgagc ttcgtcgaat tttcctagtg cgatcgttcc atctgtcgga
     4861 agatcactaa tggaatccca cgtttctttc aataaagtaa cgtttgcttc ttctgccttt
     4921 ttttcgattc ctagacgaat agataaataa taaagatctt caagctcttc cgtatcgtcg
     4981 taccactgga tcaggcgaag cgtttgctta gtatttgctg catttttacg atgtttcgta
     5041 taatttaagc gttctgctgc ttcaaaaatt ttttgctttg tggctaagcc aacggaaaga
     5101 tcaggatcat agttgagaac tcttgaaaca gtagcaggag aaactcctgc caactgcgca
     5161 atgtctttaa ttgttgccat ttgtctctcc tcctttttta cgtttataaa attgcagtaa
     5221 atcgttcaaa cgctgcctgt cctttttcat ctcgcttgaa tactccagcg tcttccagta
     5281 cacgtgcaaa aacctgacct acagattttt gtacgacatc ggtgaccgta tctttcgtca
     5341 aatcaggatg ttcttttttt aattgttctg cccacggacg atgatattca gccatctggt
     5401 tttcttctcc taataagaaa tgctctactt ctactagttc tggtttcaaa cgaggtggta
     5461 aaacagcaag ccccattact tcgatcaacc caatgttctc ctttttgata tgttggacat
     5521 ctttatgtgg atgaaaaatc ccatcaggat gttcttctga aacattgttg tctcgaagga
     5581 caaggtccaa ctcaaagtaa ttgtcccttc ttcgtgcaat tggcgtaatc gtatggtgtg
     5641 gtgtaccatc tgctgaaaaa gctctgatag atactgtttc atccgaatat gctttccact
     5701 tttccaatac tgagttagct gcttcaatca acaatttttt ctctgttgat tgcaaacgga
     5761 tcacagacat cggccatttc acgactcctg cgatgacttg cggataatct gctagtttga
     5821 taagtttttc tatcggtgct tttgccatag gaaattcatg acgtcctgcc tgataatgat
     5881 catgagaaag aatcgatcct ccaacaatcg gcaagtcagc attcgaacca acaaagtaat
     5941 gaggcaagac ttcagtaatc cgaagcagtc tagcaaatgt ttcctttgtg attttcatcg
     6001 ggcggtgttg ttctgataaa atgatcgaat gttcattgta ataagcataa ggagaatatt
     6061 ggaatcccca gctttcaccg tctagattca ttcgaataat ccggtgattg gtgcgggctg
     6121 gatagttcaa gcgacctcta tatccttcgt tttccatgca aagcatgcag gtaggatagt
     6181 ctacttttac cgcctcgcgt tctgctgcga tttgttttgg atctttttct ggcttagata
     6241 aattgattgt gatttccagt tcaccatact ccgactctgc cggaaatttg atgttttttg
     6301 cgatggctct tgttttgatg tagtcatttt tcttacttaa attaaagaaa tactcagttg
     6361 cttcctctgg gcttttcgaa tagtgctgtg caaagaatgc attgacgact gaaggtggcg
     6421 gtgtgagaaa atccattaat tgcgcttcaa aaatctcttg ttctgctggc aattctgaaa
     6481 gaacaccatt ttctcttgcc ttagcgacaa gctgatccaa taagtctaca gattcttcct
     6541 taactggtct tacttcaatg ctttctagag catcttctcc taccatccct aaaagacgat
     6601 tctgtaaata taagcggtcc atttccatcc atccgccagc ttcgatcgca agtgtggcaa
     6661 aatcacaaat cgtttgactg attgacatat ctcattcccc tattcttcat aaccatttgg
     6721 atggctgaca tgccaatccc atgctgtttt gataattgct ttgatatctg tgtattcagg
     6781 tttccaaccc agaacctctc tcgctttttc acttgaagcg actaatcggc taggatctcc
     6841 agctcttctt ggtgctattt ctgctggaat ttcttttcct gtcacttctc tagctgcttc
     6901 tagcatttcc ttgactgagt atcctttgct gctgcctaaa ttgaagaagt tgctttcatt
     6961 tccttctttc aagtattcca aagctaaaat atgtgcagca atcaaatctt ctacttgaac
     7021 ataatctctg atacatgttc catctggtgt atcataatcg tctccataaa cagccaatgc
     7081 ttttctttga ccaagagcta cttgtaaaat gattgggaca agatgcgttt ctggtgtatg
     7141 atcttctccg atagatgcat ctgcttttgc tccggctaca ttgaagtagc gcaaagcgac
     7201 atagcgcatg ccatatgctt ggtcacacca cttcatcatt ttttccatca tcaatttact
     7261 ttcaccatac ggatttttag gattagctgg tgtatcttct gtgattggag actcttttgg
     7321 ttctccgtaa gttgctgcag tagaagagaa cacgatttta ttcacattga actcgtgcat
     7381 gacttcaagt aagatttgca taccgtatac gttgttgttg aaatacatca atggtttttc
     7441 gacagattct cctactaaag agcttgcagc aaaatggatt actccttcaa tcggttcctt
     7501 ttcaaatacg gaacgtagaa attctttatc ccggatgtct ccttcataaa aatgagcatc
     7561 tggatgtact gcttgtttgt gacctgtgag caaattatct accacagcta cttgataccc
     7621 cttttgaact agctggtcta cagcgtgcga accgatataa ccggctccgc ctagtactaa
     7681 aattgtcata aaaaacactc ctttgattag taaaattgtg tttgttgatt atatataatt
     7741 taaaaaaacg aagtttactt ttatggtgaa aacaaacaaa gtaaaatttc gtaaattcat
     7801 atggtaaatt tataataaaa catttagtaa acattttcaa ggattttttg caaaggttta
     7861 caaacaaacg taaaactagc ctttttttct cttttgttcc ataatagata taacaaataa
     7921 taaatgaggt ggattcaatg aaaaaatttg tatctggaat attagtagga agtctggcaa
     7981 cagccgctgc cgttgctggt ttggtcgctt ctgtgaaaaa aacagtgatc gatccaattg
     8041 acgaaaaaga agcaatgatc gaagaaaacc gtaaaaaagc aatgcgcaaa cgcgtttctc
     8101 gttaataatt atttaaaaac acgcatgaag acttgtgaaa gaacagataa gtcatcatgc
     8161 gtatttttat ttgtcagtca atataagttg tgctatttta tctgcctgtc tcagattcca
     8221 tggatctgta taaggtctag ctactgctgc agttagccaa tccacacctt caacaggtat
     8281 tccatagcaa taaaagtcag actggatttc ccctgaagca tcaatcacgc gattcgtacc
     8341 tcgctcaaca tctatagctt ctgaatgata acttcctgta ttatctgaaa atgaaaaagg
     8401 gcgaacaagt ttgttttcct tcaaattttt tatgacagga ttacttgaac gagaaaagct
     8461 tgtttgtggc aatcgagctt caatcaaata gcgactgctg atttctttat cttgtagaga
     8521 aataacatag cggttgtgct tttgctgaat atcaaatggc ggttcgaaca aatgaaaaat
     8581 acccgcatct atcagtgctg ctaattcttt cgtccgaatg actggcggac cgatcgttaa
     8641 aaagctattt agatgagtaa accatcccca tagtatttct ttgtattcct ttgcagaaaa
     8701 aacgtgccat tcaatagcct gccggattgg atcacgccag tcttttaatg catcaaatgt
     8761 tgaagcaaat gctcctgtgc aattgccttt ttccgcttct tttatctgcc cttcaataaa
     8821 tgctcggcta gcttctgcaa aggactcatc ctgataggta agaggaattt ctaaatacga
     8881 ccaatcccat ctataaggga ttagtgctgg atgctttccg agacactcct ctgaagagag
     8941 ggagagaaaa tcatgctcaa aatttttctt tggaatcggc aaatgatatt cttcgatgat
     9001 tttcttgtaa taaaacaact caacgtcttt tttcagatag tcaaaaaatg tttcgccagt
     9061 caaaaatccc gactgatgcc attgctccag gttttctttt gttagtaacc gtggccatgc
     9121 cattgatccg ccttgctttt ggtttcttcc tcgagggtaa taagggagcc cccttcttga
     9181 tccactgtat acaatcggtt ccttgcctga tggatgatag acaagttttc ctttttcgtt
     9241 ctcgaagcgc ccttctcttg atactgtaaa caatccgaca taatcaaaaa aacttaagcc
     9301 tagacctctt aaaaagacat tttctttagg cggtatcttc gaaagatcga catctgcagg
     9361 attagaagga agctgataca gtaaatcatt tttctttgcg tagtctaccc acactctttc
     9421 ttcttctgtc tcatcatttt cccagtgacc gcttgctaaa acaagctgat cgatagaata
     9481 gctttgctga tctgtctgta aaagataacc atcttttctt ttgctgactt tttttactaa
     9541 agaataaaca aactgaatag aagctggaaa ttcttcttgc aactttgaga aaaaccagcg
     9601 ttgatagagt ccgtataaac atcgagtaga ttgctgatct ttttccagac gattgatttc
     9661 ttctataatt agttgcttat ttttggttac cgtctgttcg atataatctg tagcaggtcc
     9721 tttactccat tgaaacaagt tgggtccctg tgttaccaat cctccactgg acaatgtttc
     9781 atctgtaaaa agcgtcactt gttgagaaac agaattcatt aataactccg gtggctgatt
     9841 caaccgccaa actcgacctc caggaccatt gggatcgaaa agaaaaagtt caagagaatg
     9901 tctgtcctga ttattatgga gaatcctttc tgctatactt aatcctctag gtccggcacc
     9961 aatgatccct attttcattt gcatgcctcc atttcgataa attttttcaa taaaacagca
    10021 tggttctgat gttcattttt tgcaccataa acaaaagtaa tgactggatg ttctttcata
    10081 agttgactga gtttaaaaat agcttgcttc ttttcttctt cgaaaatcaa ttcttgctgg
    10141 tatttctttg caaatgaagc aaatcgttct ggtacatggt caaaagtttt gcgtgcttca
    10201 ttactaggtg caatttcttt caaccacaga tctaactgcg ctttttcttt tgaaattcct
    10261 cgtggccaga acctatccac gagtattcta tagccatcct ctttttcagc atcaaggtaa
    10321 acacgtttta atttgatcat agttgtttcc ttctttctgc cttttcgctt agcttatata
    10381 tgtagataat agcacaaaat aaaaaaaatg aacggcacaa ctatccattg cgccgttcat
    10441 gtgatcttgg tcaaaatcta atgattgatt cggtttttaa agctgtcata caatcatctg
    10501 ttttatactt ttttactttt gatttttcca gaccatccgt catatccacc cttaagtaaa
    10561 taaatctctt gataacctgc tttacgtaat ttatttgctg tacggatgct caagcttttc
    10621 ttttgatcgt aaatatagac aggctggtct tttcgaatag atcctaatga atctttcaaa
    10681 acagtgtaag ggatgtttct tgcacctaaa atatgcccag catcaaacac atctttttcg
    10741 cgaacatcga ttacttgtgc ttttctcatt ccttctcgaa attcttcttc tgtcaaagtc
    10801 gttgctgaac gcttggccat tactcgtaga tagacttccc aaaagatgat tgcaagcaca
    10861 atcaatagca aaatgatatt aattacccat aaaaccatgt aatgcctccg tttttttaag
    10921 caaatttcaa ggctaatgag gcagcaccaa tgacaccagc ttcattacca agctctgcta
    10981 atttgatttt tgttgattct cttacttgcg ggaaagtaaa ttctttaaaa tatttctcta
    11041 cacggctacg taaaaattct cctgctgccg atactcctcc acctaacaca atacttgacg
    11101 gattcaatgt attccccaag ttggcgcaag ctaaacctaa gtagaagcat acgcgatcaa
    11161 caaccatcaa tgcaaacgga tcgtcttctt gagccatttc aaagatatct ttacttgtta
    11221 cttcttcacc gttgtctaac attgctttca gttttgaatc tcctgcgtat tcttctgcca
    11281 agtaacgacc tagacgtacc acaccagtag cgctagacac agtttctaaa catccgcgtt
    11341 taccgcaagt acattcgaac ccttcaggat ccacagtgat atggccgatc tctccgcctg
    11401 ctccgctcac gccgtgaatc aaatttccgc cagcaacgat accaccgcca acacctgttc
    11461 ctagagtaac gaatacaacg tctggatcgt tttctccagc acctttccat cgttcaccta
    11521 aagcagcaac attcgcatca ttatcgatag cgaatttgat tcctgttcct gcctcgatca
    11581 gttttttcac aaattgaact tctgtccagt ttaagttata cgcaccaata accgttccag
    11641 cttcgctgtc cacgcttcct ggggtaccca tgccgattcc gataaagtct tcggctttca
    11701 tattatataa attcaaacga tggttgattg attcaatgat ttcaggaacg atatgcttac
    11761 catcatccaa aatattggta tcgatgctcc atttttgttg aatctctcct tctggtgtta
    11821 aaattgcaaa ttttgctgtt gttccaccta aatcaatccc aataatcttc ttgtccatta
    11881 caaatttcct ttctttcggc tttcttcaat tcggtgctct ctttttaata tgcgccacgc
    11941 tgtcatgtat gtatcatgat caatcaaacg gctatcatac agattacgca attcgatagt
    12001 cattaattca atgtcataga tgcgtgcacc cacatagata aagatgccaa attttttgaa
    12061 cagctgctgt acatcatata agctctccat ctttaagcac ttccttcata ttatacaagt
    12121 aaaccatatt tttttaaacc cagtaaaata catattacaa aaagaaatac aaaaactagt
    12181 gtagaaagaa tcctttggtg tatcgaatag cttgtctgtt gtttcggtag acccaaaaca
    12241 ttccctaaca ctaatccgcc aattagccca ccgacatgtc cccaaacatc tacagaggta
    12301 tccagaagac caaaaacaaa tgtcaacaca ataaataaaa gaaaatgtct gactagctgc
    12361 tggatcgctg ggttatgttt aaaatgaaac cctaagataa acaacgcacc gaacaaaccg
    12421 aatatcgaag tactcgcacc accagacaga acattcgctt cgttaaaagc aaagcttgct
    12481 gtatttccca ttatcccgct gaacatataa atcaagaaaa aacgccaatg gccatagatt
    12541 gcctcgattt gctgccccat aaaatacagt atcagtgagt tcattacgaa atgcatcagt
    12601 ccaaaatgga tgaagattgg tgtcactaaa cgccaccatt cattgaagtg aacaagataa
    12661 ggaccataca ttccggcata ataagggatc tcaagtcgcg gaaataattc catcatgagg
    12721 aagaccgctg tctgtatccc taaaaaagca taggtccata acggtcgtct taaccatgca
    12781 cgcaatttta tttgctgttg ataattcatg aaaacctccc atttcatttt agtcaataaa
    12841 caattgttgg atcttctgat caaagctctc cggttgccat tgttcattca attgttcacg
    12901 aaaaacaagg ctacatgttt ttttatgaaa attatttaaa taccggtcgt aataaccacc
    12961 gccaaaaccg attcgataac cggctctatt gtagataata cctggtacaa tgaggagatc
    13021 gatcatctcc ggcaaataga gcttctctga ttgtggctca agaacaccaa aactcgaacg
    13081 tacaagaacg gactgttcat ccatttcata aaacgccatc tgacgtttcg gcaaagtttt
    13141 aggtacaact attttctttt gttctttttc tgctcgttgg ataaccggct gtgtatccag
    13201 ttcaaattca ttcgataaag tcgtccctat cacttttgct gacttccagt actcgctggc
    13261 gaatagctgt ttatagattc gctgttcttt ttcctttttc ttgccacttt tggccagatt
    13321 tttcagcttt tcgattcctt tccggcgtaa tcttactttc aattggtcca aaaactcacc
    13381 cttccttctc tcataaaatg gtataggaaa aaaagaaaaa tagcaatcga actttcaaat
    13441 aaatgcataa atagaataaa aagaagacct agaaatgatt ggccttcctt ttttaataaa
    13501 tttcaactgt tcagcgcgtg tttttgtctg ataaaaagtg catacagaaa atatggcgca
    13561 ccaaaaaggg caatcaaaat tcctgtaggt acttccccac ttggtagaat cactctagca
    13621 accatatctg cactaagcag aaaaatggaa ccgaacaaac cgctcagaac aaaggtccag
    13681 ttattttctt tacgtacgac tagcttcgca gcatgaggag ctagtagtcc tacaaaagat
    13741 aaactacctg tcaccgctac actaccgctt gccaaagcaa cagctaacaa caaaaggatt
    13801 ttttgacttt tagccacgtt aaggcctaat ccttgcgcgc gttcttgacc aaatgatagc
    13861 aatccaatcc tctgtgcata aagtcctcct agcggaatta agataaccgc ccatggaagc
    13921 aatgcccaaa cataaggcca gctcgcaccc cagattgatc ccgctaacca tgcattcaca
    13981 aatccataat tttccttaga taattggatc gtgcatagca atgttaatgc agaaaggccg
    14041 gcattaacgg caataccgga caataagatt cgattcatac ccaaaaagcc atttcgatca
    14101 taggccgcaa gatagacaca gcaagcagct aaaacaccac ctagaaaggc tacaactagc
    14161 aatagaaacg gcagcgaata gtgcgaaaag aaacctaaat aaagcatcac taaaaatcct
    14221 gctccggcat taatgcccaa tatactagaa tctgccaatt catttcttgt tactccttgc
    14281 aagatataac cagaaacgcc aagacaagct cctgctaata aactgatcag taatcgagga
    14341 actcgaaatt ctctaacaat cagcgccaaa gaagatgatt ctttccctat gaacgtggca
    14401 aaaagctgat ccaggtaaat aaaaggcgta ccaatcatta aactacctat agacaggatg
    14461 acaagtacaa aaagcaagaa atagaacgtc caatatattt ttttcaccgt tgttccctcc
    14521 ttacttgtat cagtaaaaac ggaacgccaa tcaatgcaat gactaatcca aaaggtgttt
    14581 caaaaggggg attgatcatc ctcgctgtaa aatccgccaa cataaccaat ccaccgccaa
    14641 ctaaaaaaga gacaggtaga acataacgat agtccgttcc aaccattccg cgaacaactg
    14701 gcggaaccaa cagcccaata aatgaaacag gtccgactaa cgaaacggca atggctgcta
    14761 acactgcaac tagacagaaa acaatcaacc gcgttcgttt aggatgactt ccaagagcta
    14821 atgcatgtgt ttcactcaaa tgaatcaaag atattgcttt tcctaatgaa aaagaaacca
    14881 aaacagcaag tatatataaa ggaaacaata ctgctaattg tttccatgtg atattggcag
    14941 ctccgcctac aaaccaaaaa gcaagatcct gctgcagatt aaagaattgg gtaagtgctt
    15001 ggctgatagc agtaaaaaaa gaactaatag ctacgccagc taaaatcagc tgtaaaggat
    15061 tcatgcccat tttcgaaaac tgcaaaaaac caaatacgag caaagttaca gcaaaagaac
    15121 ctgccaatga cattagaaac acagaaaaat cagaagcttt aggccaaaaa gcaaaactaa
    15181 gggccattgc caacgaagca cccgcgttga ttcctagtaa gcctgaatct gctaaaggat
    15241 ttcttgtcac tccttgcatc aatgcaccgc tcaatgcaaa acaactccct acaagaaatg
    15301 ctcccgccac acgaggcaaa cgaatatttc gtatgatttg gtgactttgt tttgatctat
    15361 caaaatcaaa caacgcctgc caaaaatctg cttttcccac agtcgctgcg ccatttgaaa
    15421 gagcaacaaa aaaaataatc aaaagaacaa aacagaccaa aagcaggagt ctatttttct
    15481 tcatagtcct cataattgat cctcctgctc ttttggcaac aaatcatagg ttagaaaaac
    15541 tggtttattt gttcttggaa gcgtgacaat cgttgcatca atctcaaata tttttttcat
    15601 acactctttt gtgatcactt cttcaggtgt accttctgtg atcaacttac cgtttttcat
    15661 tgctaataaa taatctgaaa aacgtgaagc atgattaatg tcatgaatcg acatcaagat
    15721 cgttgtttgt ttcatttgat tgatttcagt caataactgt aggatctcta attggtgtgc
    15781 cggatctaaa aatgttgtcg gttcatccaa gatcaagata tctgtttcct gagcaagcgc
    15841 catagcgatc cagacacgct gggtttgtcc accagacagc gttgcgatat cccgcatacg
    15901 aaatccagct agacctgtag catccaacgc ccactcgatt gcttcatgat cttgcgtgtc
    15961 catgccactg aacagttttt gataagggta tcgtccgtaa gatactactt cttctactgt
    16021 catacctgta gggtgttcac tagtttgtgc taataatgta attttttttg caagttcttt
    16081 tgaactgtat gtagagatat cagagttatt gacaatgatc tttcctgatt tcacaggtag
    16141 tatacgggta aaacttttca ataaagtaga ttttccactt ccatttggcc caatcaaact
    16201 agtgattttc ccttcaggaa tcgtaacaga catgttttca atgatttttt tgttttcgta
    16261 gccggctgca atgtcagctg ctcttaatct cgtcatttca cccatccttt cgctgaaaat
    16321 cattctcaat tatgacgctg tttcaacatt tcgtcaagcg ctactttcat atctatagcc
    16381 aaatgaagcg gttgacagcg ttcgaaatat caggaataat aaccaatgag aaagattctc
    16441 aattatgtta ggaggaaaga aatgaaatta aaaacaaaag tattcacatt atctagcata
    16501 ttgctgctgg ctggagtact gagcgcttgc tccccctctt cagataaaga aacagctaca
    16561 actgccaatt cgacgacttc atccaatagt actcatacag tcacagatac acttggacat
    16621 aaagtagaga ttccaaacgc gcccaaacgg atcatcggca gttacttaga agactatctg
    16681 attgcactag gggaaaaacc tatcgcccag tggactgttg gcagtggttc cattcaagat
    16741 tatctgcaag atgatttaaa ggatgtccca acgatttctt atgaccttcc ttatgagaaa
    16801 gtgttaagct ttgagcctga tttgctgttg atttcttctt ccgctactgt cgaaggcggg
    16861 aaatacgaac aatatagcaa aatcgcgcca acttatgttg taaaaaacgg aacagatgtc
    16921 acatgggaag aacaactaaa agatgtcggc aaagctttgg caaaagaaaa agaggcagaa
    16981 aaaatcattt ccgattatca aaaatccatc aagaaaactc gagaagaact agcagataaa
    17041 atcgagaaca agacagctgc agtgttgtgg gtgaccaaca actcagcctt tatggtcagc
    17101 aaaaatcggt caagtggaag aatcgtatac gacgatctgg aatttggtgt tcctgatcta
    17161 gtggaagagg tctccaaaaa agccacttct gactggtctg ctgtttcctc tgaaaagcta
    17221 gctgaattag atgctgacta catcatcctt gtgaacagtg acaaagatgc tgccatgttc
    17281 aatgaaccaa gctggaagaa tcttaaagca gtgacagaca atcatgtact gcaattcgga
    17341 cctgaatcta gttggctata taacggacct attgccagtc aggaaatggt caatgatatc
    17401 aaagaaaaac taaattgatt ttaactaaaa aaagaatctt cacgattagt cgtgagatgc
    17461 tttttttctt attaacaaac tagacgcata gattttattc ttcttttgaa gcaaccctat
    17521 tttttactct ttcccatttt tgaaaacgat gggcatatag attttcttga tccgttatcc
    17581 cctcatactc ctctataagg gcgaagtctg aaaaatcaat attcccaata tacgtatcgc
    17641 cttcaaacgt gctatcgatc aatgttcgcc agatccgacc agtgtaagga agaagttctt
    17701 gaaaaatccg actaccaccc gaaacataga tatcttctga gatcgtttga ctatacgaca
    17761 aaagttcatc gatcgaatgc attacttccg cattttcttt cgtgcgaaaa tttggttgcg
    17821 ttgtcaacac gatgatatgg cgataaggaa gtgaaagatc ccccattcct tcataggtct
    17881 ttcttcccat cactaaaatt cgatccattg tatgttcgcg aaagaaacgc atatcatttg
    17941 gcaatcgcca tggtaacaaa ccatttttgc cgatcagacc atttttatct tgtgcccaca
    18001 tggaaataaa catattatcc gctccttatg attttagatg ttttgagata aaaaaagtct
    18061 caattctttg accagcaagg aattgagaac tttaaaaaca ttattttgtt tcacggtgca
    18121 aagtaacttt gcgttcgcgt gggcaatatt tttgtttttc caaacgatca ggattgttac
    18181 gtttgttttt gcttgttaga tagttacgtt ctttacaaga agtacactct aaagtgatgt
    18241 ttacgcgcat gtttttccct cccattttta caagaatcct ctaagatgac tctcagactt
    18301 gattatctta ccatgatttt ttctagattg ctagtgtttt tcacgatttt gttaagtaaa
    18361 actacttgac gataaataca acggttttac ttctcaaaca aaatcttttt ctttttgtat
    18421 aaataccctt acacttgttt atatagatta tgttttttaa taaaggggga tactaatatg
    18481 ccatggaaca tgaaggactt tcctgcatca atgaaaaact tagataaatt gacaagaaaa
    18541 aaagcaatcg acatcgcaaa tgcattatta gatgaagggt accctgataa ccgtgcgatc
    18601 ccaattgcca ttgatcaagc aaaaaaatgg tatgaagatg ctagtgaatc agaaaaacga
    18661 actttcgaaa aagaaaaaaa tccaagcaag acggatgaac acgatacgaa tcctcgtgct
    18721 ggaaaactgc ttgattcaga tgtgatcgta gaatatgagg aagaacaatg gatcgtgaaa
    18781 tctaaagggg caaaaaaggc aagcaaccat tttgatacaa aaaaagaagc aatcgaaaaa
    18841 ggaaagcaag tagcacaaaa caaagaatca actttgttca tttataaaaa agatgggaca
    18901 aaagaaaaag aaatcgatta ttgaacatat gtaaaggggt tgtgacgaaa atcgtgtcac
    18961 aaccccctta tgttcgaaac aatggtgttc ctctgccaac tccgaaccat aagtcataat
    19021 atctataaaa cgggaagagc tgttttttga aattgtttct tatcactcag tgttaaacgg
    19081 taggagaaaa cctctttttt attgatattt gattttcatc aaacagtcac atagtcgatt
    19141 gcttctgctt gcggaatcgt ttcatctaca aagtagactt tataccatgt caagaaactg
    19201 aagattgtcc agatttttct ttgctcttct ttacgtcctt cgtgatggtc atctagcaac
    19261 ttcatgattt tttcttgatc aaaaaattct tttgcaaaat ctgcttcgaa taatgctttg
    19321 acttgttcgt agccgtgttc ttctttcaac caagctttga ttggcacagg gaatcctaat
    19381 ttcacacgat tcgaccattc ttccggaaga tgtttgttcg cagcttgtct gaaaatatct
    19441 tttgtgttgt tttgattcaa taaaaactta cttggtattt gttgagccaa tttcatgact
    19501 tcgatatcca ataaaggtac tcggacttcc aaagaacttg ccatagaaag tttatctgcc
    19561 tttaacaaga tatcttttgg catccattga tgaagatcga catattgcat cttattgact
    19621 tcatctttga ttccttctgt tttctcgtaa tgaacagtca tgatttcatc cacagatggt
    19681 gctgtttgat attttggtgt taacacttct gatgcttctg attcttcgaa cactcgagca
    19741 tgaccgataa aatattcttt tgcaggagca agtgattcat ataaatggat ccgtccatgg
    19801 aaatttttca tttttccgat tttacgtcct aaattatagc gtgttccttt tggtaatttt
    19861 ttcaatcctt gagcaatcac acggatgaat tttgaattcg tatggaatcc atacgtttgg
    19921 tatcccgcaa acagttcatc cgccccttct ccagattgga ctacccgcac actttgtgaa
    19981 gcaagatttg ctaaaaagta caaaggtacg caagaaggat ttgaatcagg ttcatctaag
    20041 tgatactgga tcaaagggaa tgctttgaac gacatgtctc cgtcgatcac tgctgctgta
    20101 ttatctaagt ccaatagttc ggttagtttt ctggcttcaa tcgcttcatt ataggtttta
    20161 tcatcaaacc cgattgagaa agaatgatct ggtttcaata cagaggttac ataactggaa
    20221 tcaaccccgc tggataagaa agaaccaact tctacatcac tgaccgtatg tgcttcaata
    20281 gaagcttgaa cggtttcgtc gattttttcg atccattctt gtcgggaatg cgtttctttt
    20341 gtttcaaaat ctgcatccca atattgctga atgtctagtt ttccatcttt atacgtataa
    20401 taatggcctt caggcaaacg atagacatct ttgaagaatg tttcgccgtt taatggagaa
    20461 tattggaaag tcatataagg tttcaacgct tctttgttca attctttgtt aaaatcagga
    20521 tgaggcagaa aacttttgat ttctgaacca aacataaatg ttccgttcat ttctgcataa
    20581 taatatggtt tgatcccaaa gtggtctctt gcaccgaata actcattctt ttcattatcc
    20641 caaatagcaa aagcaaacat tccgcggatc ttttgcaata attctgttcc ccattcttcg
    20701 taaccatgca aaaggacttc tgtatctgca tgtgtttgaa aaacgtggcc agctgcaatc
    20761 aattcttctc tcaaaggctg gtaattatag atttcgccat taaaaataat gatctttgtc
    20821 cgatcttcat tgtaaatagg ctgagtaccg ccctctaagt caataatact taatcttctg
    20881 aatcctaaag caacatgttt atcgatatat tctcctgaac tattaggtcc acggtg
//
LOCUS       DAZKNO010000035        20895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000035 DAZKNO010000000
VERSION     DAZKNO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.035"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2046)
                     /locus_tag="ACG8MY_000956"
     CDS             complement(<1..2046)
                     /locus_tag="ACG8MY_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0430176.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     "
     gene            complement(2059..2334)
                     /locus_tag="ACG8MY_000957"
     CDS             complement(2059..2334)
                     /locus_tag="ACG8MY_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430177.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(2696..3151)
                     /locus_tag="ACG8MY_000958"
     CDS             complement(2696..3151)
                     /locus_tag="ACG8MY_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430178.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(3305..4513)
                     /locus_tag="ACG8MY_000959"
     CDS             complement(3305..4513)
                     /locus_tag="ACG8MY_000959"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0430179.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(4625..4957)
                     /locus_tag="ACG8MY_000960"
     CDS             complement(4625..4957)
                     /locus_tag="ACG8MY_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0430180.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            5105..5980
                     /locus_tag="ACG8MY_000961"
     CDS             5105..5980
                     /locus_tag="ACG8MY_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0430181.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(6062..6643)
                     /locus_tag="ACG8MY_000962"
     CDS             complement(6062..6643)
                     /locus_tag="ACG8MY_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0430182.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(6649..7908)
                     /locus_tag="ACG8MY_000963"
     CDS             complement(6649..7908)
                     /locus_tag="ACG8MY_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0430183.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(7910..8956)
                     /locus_tag="ACG8MY_000964"
     CDS             complement(7910..8956)
                     /locus_tag="ACG8MY_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430184.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(9191..9466)
                     /locus_tag="ACG8MY_000965"
     CDS             complement(9191..9466)
                     /locus_tag="ACG8MY_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0430185.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(9709..11019)
                     /gene="der"
                     /locus_tag="ACG8MY_000966"
     CDS             complement(9709..11019)
                     /gene="der"
                     /locus_tag="ACG8MY_000966"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0430186.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(11201..12430)
                     /gene="rpsA"
                     /locus_tag="ACG8MY_000967"
     CDS             complement(11201..12430)
                     /gene="rpsA"
                     /locus_tag="ACG8MY_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0430187.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(12565..13245)
                     /gene="cmk"
                     /locus_tag="ACG8MY_000968"
     CDS             complement(12565..13245)
                     /gene="cmk"
                     /locus_tag="ACG8MY_000968"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0430188.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(13294..13929)
                     /locus_tag="ACG8MY_000969"
     CDS             complement(13294..13929)
                     /locus_tag="ACG8MY_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0430189.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(13995..15398)
                     /locus_tag="ACG8MY_000970"
     CDS             complement(13995..15398)
                     /locus_tag="ACG8MY_000970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0430190.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(15385..16416)
                     /locus_tag="ACG8MY_000971"
     CDS             complement(15385..16416)
                     /locus_tag="ACG8MY_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430191.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            16447..16668
                     /locus_tag="ACG8MY_000972"
     CDS             16447..16668
                     /locus_tag="ACG8MY_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0430192.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(16833..17570)
                     /locus_tag="ACG8MY_000973"
     CDS             complement(16833..17570)
                     /locus_tag="ACG8MY_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0430193.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(17723..19063)
                     /locus_tag="ACG8MY_000974"
     CDS             complement(17723..19063)
                     /locus_tag="ACG8MY_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0430194.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(19300..20823)
                     /locus_tag="ACG8MY_000975"
     CDS             complement(19300..20823)
                     /locus_tag="ACG8MY_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0430195.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         6001 a         4466 c         3505 g         6923 t
ORIGIN      
        1 tcgaaacgca aatcggaaag aaaagttatc agatggtttg ttcatcgtat aagattctaa
       61 caaattgata ttcctgcgat agctgttcgc agaaaacgtc gtgatttttt cagcaacttc
      121 gtattcatca atgatttttc tttcaagata ataacctctt agcatttttt tcgtaaacag
      181 actatcttgc tggatcatcc acgaagaaat caaattcccc gagttcattt ttattccttc
      241 aagtttgcgt tgctggataa aaaattgaac catcgtttca tcataaatat cagtcaagtt
      301 atctaaactt tcaataatca actgagtatt tttttgatat gtctctctga tttcttggat
      361 atcttcatca ctaataaatg tctgacgatc agcatatctt ctttgccaaa acattttcgg
      421 atggagaacg attcgcatta acctacggac aacaatgaac caaaagccga taaaagacaa
      481 aatctggctt gtgatagaat gttcgaatcg agaaagaaaa cgggagtaaa aacgataacc
      541 attagaagaa agcttgcccg aacgataact ttcatccaat ccatcccgtt cgatcgacaa
      601 aatcagtgct tgtatttctt gtacttcttg cttttcatca tcggttaaat cttccaagta
      661 cagatcacgt atccgttctt gataattttc tatcactgct tcagtggcta aaaattcttt
      721 tgtattacga tcaatttcct ttatttcttt tcgcaaaact tcaacgacct cttctaaaat
      781 catcaaagca ttcaaatccg tgcttttcgc ttcctctgtt tccgtcagca taggcaagac
      841 aatgatccct atgaccaaag tcactaagat gacacccgcc gtcagaaaca aaagcaaaga
      901 ccgctggtaa aacatcatat tattgattga aaatggcaaa ataaaaatcg tagccaaact
      961 gactgttccc tttacaccgc caaatgttaa gatcaagatc tcgttcaact gttttttgaa
     1021 ctttttactg ccatctttga atacataaaa aagactgata gaaatgaatc tgatcacaaa
     1081 aagcatgaca gagattaata cgataatcgc taataaaaga ccgtttggat ataaaccgtc
     1141 tccccatact ggcgagaaca cttgcgtcaa ttcgattcct aaaaatataa atactaatgc
     1201 attcagtgta aaagcaatag ttgtccatgt actgtgggac aagctcgaca attctgcatc
     1261 aaacacagag attttcctaa aaccagaagc ttggagaata cctgctgcca ctgcagcaat
     1321 gattccagat acatcaaatg cctctgccaa tacataggct agaaaaggaa gcaaaagttc
     1381 gatcagcaga taccctgtga catcttgtgc tgatgctttt tcgatcaaat gaattatttt
     1441 ttgtttgaac cagacaagca aaaagccgat caaagctccc ccaatactag aaactactag
     1501 actcatcccc gcgtttactg ctgaaaaaga tccggtgatc agcgcaccaa gagcaaactg
     1561 gaaggcagta acaccagatg catcattcaa aagaccttct ccttccaaaa tatgcatcgc
     1621 ctttttaggg atattcactc gtccagaaag agaactgact gctaccgcat ctgtcggacc
     1681 taacgctgct ccaaaagcaa agcaggcagc aagtgggaca cttggcaaca ggaatgataa
     1741 cgtcgcacct accccgacaa gggtcaaaat gactcctcca aatgccaaga acaaaattgt
     1801 cccgaaattt ttgagaatcg aagaaatatc tgctttttct ccttctctaa ataacaatgg
     1861 tgcaataatc ataactaaga acaattcagg ttcaaaatcg attgattgtc cccactctgt
     1921 cagacctaat accactccaa ggaaaatttg gatcagtgga gctggaatca ttggcaaaat
     1981 gcggctagct atgttcgata aggtgatcgt tactgcaaat acaataacta actcagcaaa
     2041 ttccatcgtc catccccttt attttttatt ttgtgtaagc atttttaaaa attgttcttt
     2101 tttctttaaa attttggttt cgactacttc aacctgtgtt tggttcccct ctaattcaca
     2161 ctgtacacgt tttgactcca aacgaagcag atcatgtttc agacgttcca actgacgata
     2221 catcaatata tttcttttac gcaacttaca aagtatttga cggcggtcat ccatcgaaag
     2281 ggcgccatag cgatcatgca acagtttttt ttgttgttca aaactctctc tcattttcca
     2341 gatctcctat acacaaactt ccatcattat agcaaagttc tttttggatg aaaaatattt
     2401 cattgtttca tcgtttattg ctagttagaa atttccataa atttttcaac gcctcattct
     2461 attccaaata gtatcagata tcaagtaaaa cttaagaaag ataaaagttt ctgacaaaag
     2521 gatgtttact ttcccattcc tgcctttctt gttcttttat cacaacaaaa aaatggggct
     2581 gcgacaaaaa tcttgtcaca gtcccttatt ctgaataaac ggtgtggaag agacagccta
     2641 tcagtagtaa taagacacat ctctttcaca gcctcatttt tgcgtaaaaa tcgatttatt
     2701 cgaaaaattc cccgtataca tcttctacac gttgatctgg attgatcacg atatacaagt
     2761 tacctgtttt ttcgctgatt tttgttgatt gatattgatt gtctaatcct tctgttcctt
     2821 tatctagata tgggaagtac acaagatcag gtgtgccatt tcggtaaagg atttccattc
     2881 gctcgatatc ttcgtatttc aatgcgcgat taaacatgcc catttccagt ccgcctaggt
     2941 tgatatcatg tgtcgcaact gcgtcccctt catggaagat ttcgatttta aatccagcac
     3001 acggatggat ttcaacaaat tcactgccat tgattcgtcc aaaacttgtt gtgatttgtt
     3061 tgatccaaag atcgccgata tgacggcggt tgatcgtcca ggtttctcca tttctaaaat
     3121 aaaattttag cgctgtcatt tctttactca ttaaaaagac tcctttatga tgtaatttat
     3181 tcttgtacta cctcagttta gcacaaattt atgtaagtgg tcacatctta tgttttttct
     3241 tctaatgacg tatctgccca ctttaagtag ccaacaaagc tgagcagata cagtctagtg
     3301 ttttttaaat caatttttct ttcgataaaa aatccaaaac ctgtttttta tcatttttta
     3361 ttctttgttc tacgacggct ttttcagcta attgcaaagc ttcactaacc cacttaccag
     3421 gtttttttcc agtttcttct agcaagattt taccatctac tgctaattcg cttaatgact
     3481 tgataggtaa acgatcatag gattctccta cggcatccat ctgaggtgtt tcgtcaaaat
     3541 agatcatcaa ttcttctact tgcagtgcat gttttttgcc taagaagtac aattcgaatg
     3601 gttccaaagc tccagtttct cgttgtcgta gtccttggac aagtgcttga acgtctttaa
     3661 tcatttggtt agaacacttc cacgatttca aaaaggaacg aatctcattt tcttttagat
     3721 ccagttggct gatcaataat gcccatgctt ggctctcttc tttcaatggg acggatggta
     3781 attcagcaaa acgtaaaagt gcttcgcctt cttctttcaa accgggacag caaatgtagc
     3841 actctgtttc aataaatgca tggattcccg cggcccgttc atttcccaag agcatcttga
     3901 tgaattccac atttatacgt tctacagata ttttacctaa tagctcgtgg ttttctttga
     3961 tggcgtagaa ggtttcttcc tctaatttga accctagttg actgacaaaa cgcaacccgc
     4021 gcatcattcg taaggcatct tcatggaacc gctcatgagg attgcctaca gctcgcaaga
     4081 tctgttcttc tagatcttgc agaccatcga acaaatcgat gatttctcca tcttctttca
     4141 aagcaaacgc attgatcgta aaatctcttc ttttcaaatc ttctgccaac gaacgaacga
     4201 attcgacatg atctggtcta cggtagtcct gataagttga ttccgtccga aatgtggtga
     4261 tctcatattg ttcatcattt tctaatacca acacagttcc atgttcgatc ccaatatcta
     4321 tcgtttttgg aaaaagctgt ttgatttctt ctggaaatgc gcttgttgcg atatccacat
     4381 catggattgg ctggttcagc aagatatcac gaacacttcc acctacaaaa taagcttcat
     4441 atcctgcatt ctctaatttt ttcagaactg gaattgcctt ttggtattcc agaggtaatt
     4501 gttttaattt catttttcca acctttcctg cgtgtttctt gtcctgagat aaagactcag
     4561 ttgcatggtg actgtttgac aagtccgttt cagagtcttc aaggccagga ccacttcatc
     4621 ttgtttactc gtccttacgc tcaaagcgat tatggtctct ttgattgaat ttttccatgt
     4681 ttttttcaaa aacttcagtt aagtcgatat tcaatgaatt agccatgatc atcgtaacga
     4741 ataaaacatc accaagttct tctgctaccg agttaactgg ttcagaagcc tttttgcttt
     4801 tttctccata agtgtggttg acttctcttg ccaattcacc tacttcttca gttaatcgag
     4861 ccatctgagc taatggagag aaatacccca ccttgaattg ctggatatat tcatccactt
     4921 cctgctgcat actgtataaa gaacgctttt tatccatctt tttccctcca aaactatctt
     4981 accaaaactt taacgcaaat cctagataag agcaattgag aaaatcgaat ttccatgcta
     5041 catttaggaa ggaatttcta aatggaaatt ctgtgaaaat acataattga aagctggaat
     5101 tcatatggtc aaacgaaaaa cagtaaaaga agttcttttt atcattatag gaacaagtat
     5161 ttatgcattt ggtcttgttt acttgaacat cgctaatcat ttagcagaag ggggagtctc
     5221 agggatcacc ttgatcttaa gagctttatt tggtattgat ccagcttata cgactttatt
     5281 aatcaacatc ccattgattt tactaggagg aaagatcctc ggtaaacgtt ccttggccta
     5341 tactgttcta ggtacacttt ctttgtcatt ctttttatgg atctggcaaa gggttccact
     5401 agaaatcaat ttacagcatg atctactgat cgtttctcta cttgcgggtt tagttgcagg
     5461 tgtaggaagc gggatcgtct accgcatggg tggaacaaca ggcggcagtg atattatcgc
     5521 tcggatttta gaacaaaatt acggcatcac tatggggcgt tccctattgg catttgatat
     5581 catcgtctta cttgcttcat taacctatat cgacttgaaa cgtatgatgt atacgttgat
     5641 tgtttcgtat gttttcagtc gtgtgattga ttcgatctta gatggcggat attctgcaaa
     5701 aggtctactt gtcgtctcca acaaaagcga agagattgca ccactactga tgaccggtct
     5761 gcaaagaggt gtgactttcc tttctggaga aggcgcattc tctggtaatt caaaaaaaat
     5821 catctatatg gtcgttagct ctagtgaatt aactgaagct aagaggatcg tccacgagat
     5881 tgacgaaaaa gctttccttt caatcatcaa tgtgcatgaa gtggaagggc aaggctttac
     5941 ttatttgaag ccacagacaa gattactgaa aaaactataa gaaaaaagac aaagataggc
     6001 tatgaggaaa gattccacat ccagctgacc tttgtctttt ttagtactat actattttta
     6061 actaattgtt atgttcactt acccggttac ttttcttttg tgtctgcaaa aggatctttc
     6121 ggttaacttc atcagataat ttcaaaaaag catccctatc tccatcttct agtgcttgat
     6181 cgatttgagc ataaagcatt tttagttttg cctcttcttc tttttcttga aaatatcgtt
     6241 caattaattc agtatcttct tcactgactg aagaatccca agaagaaaat ggattgtctt
     6301 ctaagataga tagatattct cttgtctgcc aagcattttc gaatatacat tccacatata
     6361 aaggcttttt ccaattcaaa cggatttcat gaaaaatctg gtctgtgtcc gtaaattctt
     6421 ttccctgcaa aaacaatttc atgggttccc cttcaacaga tagatcactg atctgcagtc
     6481 ctcgatccgc aaaattcgcc ccttcaacaa agtggacatt gcttaaaatc gcttcatgat
     6541 tggcaagata attcaagatc caaaaaactt ctcttcggct aaaagataca tgattgacca
     6601 tccacgttaa aaaattcttc ttttcccgta catcaattag catttttctc acctctctgt
     6661 taggtcatct aataatgaca gtgcctccat atctcctggt tcaagtgtta aataataagt
     6721 caataattct tgcgctcttt tcagctgccc ttcttctctt aagaaaaagg catactcttt
     6781 caagaaatct ggttcatgtg atagttcatg ataagcctgt tcatagtgta cagctgcaac
     6841 ggtaaagtct tctaattcat tataagcatg ggctaggttc cattctgcat aaggattagc
     6901 agtttcttcc atctgattga tcacttgtat cacatcttca tatctttcat catccaagta
     6961 aagattactt aaagtaagta atgtttcatc ttgtttgtct cctacttcta aagctttcag
     7021 taaaagttct tctgctcgct gtctatcatg cagacggaaa gcattttccg aggccaggtg
     7081 ataaagttcg acttggtaag gattttcttt gattccttct tctaacactg tttgtgcttc
     7141 ttcaatcatt tcttcttctt gaagagcttg tcctagatat agatataacg attgataatg
     7201 cggattaact tctcgaagtt cttccagata gttgattgcc tgctgatttt ctttcaattg
     7261 aagattcgta aaagctagct ggaacagtcg atcatctgtc ctttcttctt tcagtgcttc
     7321 ttctaaaggt acgatagctg cttcgaattc tccttgcata cttaaggatt gtcctaaacg
     7381 ttcctgcatc gagattcctg aaatttcatt tatcccgctt gccaacaatg tttcataaac
     7441 cactgctgcc tctttgaaac gatcaactga aaaatacaat tcagctagcg caaactggat
     7501 caatggttca tcaggaagca acccagctgc ttctttcaat tttgcttcac tcacttcagg
     7561 gattccgata acttggtaca aatcagctat cgccaataag ctttgcggat agtaatcact
     7621 ttgcttatcc actttttcca ggtattcaaa agctaagtcg atctcattat cttcgattgc
     7681 aatttctgct aacgggatgt tcagtccgtc attttctggg tattggttga tcagttgttc
     7741 aaaaatctgt tttgcttcga tcaaaaagcc aatagccagc aattcttcgc ctagttcagc
     7801 taatatatct gctgggtctt cttttaatgc tttttctaaa aagatttgtg cttgtgacaa
     7861 gtcttcatct gataaagctt gcaacatttt ttcgctattt gtattcattt caattccctc
     7921 ttatctcact gacgatttgt tgaaataccg tcattgttct caataattct ttttcgtttg
     7981 gatgttggta tccgtctatt atacctattt tttctaaaac aagtattttt ttgctttttt
     8041 ctgccaaaaa aagaactttt tccaaatact ccatctttga aatctgttcc agccagtgga
     8101 ttggatagcc aagcaagtcc agccacacaa aaaagttttt tagttggaaa tcaaaaggat
     8161 atgcttggag attccacacg aaatgaaaca tcattcctaa aaattttttc atactagcag
     8221 aaagtagatg gccattttcg gtcagataaa aagcttgttc aaacagttta ccataatttg
     8281 acaggttttc tgcctctaat tgataaaaat agatcatttc ttcgatcatc ccggcaaacg
     8341 gccgagtcgt taattgcttt ttatttggat agtttttata cagattttgc aaaaaacgat
     8401 gatcacaaat cattccgcaa cagataaaag tcaaaaagtc aatctgcttt ccgtccacct
     8461 gccgctcgct gattgtttga tcataaacga tgcgttctgg cagattgatg gtttgcagca
     8521 ctacacgatt tggtagctgc aaaatcgtcc gctgcggtgt taatgctcgg cttagagaac
     8581 ggatcgatac agggatcatc catagtgatg aatcaagggt tgtatgtttt tgaaaaaaac
     8641 ctgccagatc catgatccct tcatttccaa atccgatgat aaacatggat tgattttcgg
     8701 gatagcgttt gacgaaatca agcaggttct ttaattctgt taaatgattg cattgagtgt
     8761 tggcacaaat ataccagtca ttgtttgctt gatttgcaaa taacgggttg atcttatctg
     8821 caaacaaatc ataatagcgc tgattcgtta aaaataagat tggctgatca atggaaagtt
     8881 ctcttagctg actgactagc gtttctccgt aaaacacttt cgtcaacgca tcttctactt
     8941 gataatcaag ttccatacgc atccctcctc ttcttatttt tattttacca ttagtaagca
     9001 aattctagaa aagataagag caaagctcac ctcataactt gcaaataaat aaaaattaaa
     9061 taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc tcttatttat
     9121 tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca caagcgcttt
     9181 ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat gcaggtactt
     9241 tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg cgagctgcgc
     9301 gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg ttagctaaag
     9361 catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt ttagttaaat
     9421 cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat agattcacct
     9481 cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt ggtccagaag
     9541 tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat atgaaccgct
     9601 taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa cgtaacaaac
     9661 gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct attttctcct
     9721 acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga tttgattttc
     9781 taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa cgacaaatgt
     9841 aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc ctttatctgt
     9901 tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag ccgaagagat
     9961 acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt ccaaccgttg
    10021 tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga attcatcacg
    10081 aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat cccatttatt
    10141 cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc gcttgtcttg
    10201 ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac ggtcaatggc
    10261 acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc cgcgtttacg
    10321 cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga agtgggtgtc
    10381 gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt ctcctaaaat
    10441 cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa acttaatcgt
    10501 gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat ctaaaatatc
    10561 accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc ctaaggaata
    10621 aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg ctaaaatcac
    10681 tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg tcacgccttc
    10741 gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt ctgcttgatg
    10801 cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt cgatcacgct
    10861 gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta ctccaggagt
    10921 atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag acttaccgac
    10981 gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc ctcacaagtt
    11041 tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat tgtcaattaa
    11101 acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa aactgagaca
    11161 aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca gaatcatcgg
    11221 ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc atagtaaatc
    11281 cagtattttc ttctggtaat tcataagact caacattttt tggttcttct tctgaagctg
    11341 gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga tggatctcta
    11401 atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc gcaatatgtt
    11461 tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact tcaacaaagg
    11521 caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact ggagcttttg
    11581 tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa acacgttctt
    11641 gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc aatacatcac
    11701 ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg tcaattccgc
    11761 ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg agaacatctc
    11821 cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt tccactaccg
    11881 ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt ttaaagctca
    11941 atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc attgatgcag
    12001 ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca tttgtaacag
    12061 gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa acttttttag
    12121 catctaaacg gcgttttgaa agtagatagc tgccgttttc tttatctttg ccgatcgtac
    12181 tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata tcttctaccg
    12241 gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct tcgattccga
    12301 caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg ccaactttta
    12361 cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga ttttgatcaa
    12421 attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg tactgttttt
    12481 agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt gaaaaaacaa
    12541 ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca aaacaacagc
    12601 ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac gaacagcatc
    12661 ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc tttcctgtat
    12721 ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct ctttgaaacg
    12781 tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg cattcggtaa
    12841 tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc ctatttttcg
    12901 ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag aaacagcttt
    12961 tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga atacatgctg
    13021 tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa ctagcccttc
    13081 ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc gatacatggc
    13141 accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa cggtactttt
    13201 cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat aactcctttt
    13261 tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt cttgaccagg
    13321 gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat cagtggtgat
    13381 ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg ttgttgaacc
    13441 ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat cccctgcttg
    13501 ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc tttctgacgt
    13561 tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt ctactgttga
    13621 agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg atgtcgaagt
    13681 ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc agaaataagc
    13741 acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa gaaataatgt
    13801 atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat ctgtatcgta
    13861 gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat gtctatcttt
    13921 tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag gaaaaagtgt
    13981 cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa aatcgtttcc
    14041 caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc tgcttttgct
    14101 ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa ataagctaag
    14161 atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg caggcggaat
    14221 tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt ttcagactgc
    14281 tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctc tgtggcatat
    14341 tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa gaaataatgg
    14401 atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg atctctgcct
    14461 gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata atggaccaca
    14521 taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat gagaaactgc
    14581 agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc aagaccaccg
    14641 tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc gacttctttt
    14701 tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg ttcaagttct
    14761 tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt gactgattgt
    14821 ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc tactaaatct
    14881 gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg gatttttcct
    14941 aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg agcctcatct
    15001 actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg attagtcaac
    15061 atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac gtatcttctt
    15121 tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg tttttgtaat
    15181 tgcgtcactt gatcttccat tagagaaagt agcggtgaca cgatgatgac cagaccttcc
    15241 attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg caaaatagcg
    15301 agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg acgaaatgaa
    15361 tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt cctttctcaa
    15421 tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta cattccattc
    15481 gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga ttgtttctgc
    15541 agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa taagatgatc
    15601 tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg tctgaccatt
    15661 cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta ataatgaatc
    15721 gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca aaaggtggat
    15781 acatctgctt tgttgcagat aaaccgctga ctcatcattg ttttttgcca gctgataagg
    15841 aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg ctgcttcttc
    15901 tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca aaatttcacg
    15961 tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag gacttgtctc
    16021 tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa cagcaaattt
    16081 aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca aaccagtatg
    16141 ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg cttcattttc
    16201 atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa acttatcctg
    16261 cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc cataaataag
    16321 tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc gcagcttgtg
    16381 tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa gttaggatgg
    16441 ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg cggtctttgt
    16501 caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt atttgcccaa
    16561 gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt aaaaaaagcg
    16621 tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat ataaacgaaa
    16681 ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct gtttctcttg
    16741 ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt aattgactag
    16801 ttttttcacg gaccctatag gacccattta tattatttca actttttcag ccagttttcc
    16861 aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc tccatctcct
    16921 ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga tacatactct
    16981 gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa tactttatct
    17041 tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc ttttttgtcg
    17101 ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt cttattgaca
    17161 taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc ggaatttgga
    17221 aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc aagtggcatt
    17281 ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat actataactt
    17341 tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc atgattcttt
    17401 ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac caacacttgc
    17461 gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc tccaccgagt
    17521 aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac tttctcccac
    17581 cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt aaaaaaataa
    17641 gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca tttgaatatc
    17701 cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct tctgcataaa
    17761 tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg tgcataaagt
    17821 caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta cgagcaaaag
    17881 tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga tatgtcccca
    17941 atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg aagtcaaatt
    18001 cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact aaatcatctt
    18061 tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct ggtgtgattt
    18121 ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt ttgcccacga
    18181 cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt gggccaaaat
    18241 caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct tgtgctgcat
    18301 ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct tcgaaatcga
    18361 attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag taaggagctg
    18421 catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc gttcctggtc
    18481 ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat tcagcatctg
    18541 gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa caacgccata
    18601 aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc acgccagcat
    18661 caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca tcttgggatc
    18721 cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt tcccaattat
    18781 tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat tcatctccag
    18841 agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta aatcctagct
    18901 tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg aagggatgtc
    18961 cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg ctcaatgatt
    19021 ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta tcctcctaat
    19081 atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca aataatgacg
    19141 ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa gaaaaaacac
    19201 tcactgaatg gagctattga cttatccacg tgcttatcga tagccgttta tcggtttctt
    19261 cattcttatg atagaccttg tacctgtatt tgtctaggtt tagaacattg ctgctacacc
    19321 taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa atttccacca
    19381 cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag atgttggtgt
    19441 aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat ccttaccgac
    19501 gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta agccggaagt
    19561 tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga tgaatacact
    19621 tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg tgattaatcc
    19681 attgttcata accacttgga taccacgagc aacagctact acgattgcta caccgatcaa
    19741 atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc cataagtgat
    19801 tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt accagtcacc
    19861 taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg ttaaccagtt
    19921 tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa tcaaactgat
    19981 gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt ttttcgtgat
    20041 tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt tacgttggtt
    20101 gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat acacatatac
    20161 aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac cttcacccat
    20221 cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg ttgaagctag
    20281 acagcctact tgagacccta ctaatacgat tgctacagct actactgtat caaagcctac
    20341 agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg ccattccgta
    20401 agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt tttcttttcc
    20461 tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt tgttgataat
    20521 acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag caggtgtttc
    20581 tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca tgaaaatatc
    20641 ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga tgttgcctgc
    20701 atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga ttactgcaat
    20761 aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt ttgtttgttc
    20821 catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa tgttatttta
    20881 tcaaaaagag tatca
//
LOCUS       DAZKNO010000036        20216 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000036 DAZKNO010000000
VERSION     DAZKNO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.036"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(242..1267)
                     /locus_tag="ACG8MY_000976"
     CDS             complement(242..1267)
                     /locus_tag="ACG8MY_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0430196.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1542..3227
                     /locus_tag="ACG8MY_000977"
     CDS             1542..3227
                     /locus_tag="ACG8MY_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0430197.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3241..4524
                     /locus_tag="ACG8MY_000978"
     CDS             3241..4524
                     /locus_tag="ACG8MY_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0430198.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4594..5463
                     /locus_tag="ACG8MY_000979"
     CDS             4594..5463
                     /locus_tag="ACG8MY_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0430199.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5460..6296
                     /locus_tag="ACG8MY_000980"
     CDS             5460..6296
                     /locus_tag="ACG8MY_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0430200.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6322..7389
                     /locus_tag="ACG8MY_000981"
     CDS             6322..7389
                     /locus_tag="ACG8MY_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0430201.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7386..8213
                     /locus_tag="ACG8MY_000982"
     CDS             7386..8213
                     /locus_tag="ACG8MY_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0430202.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8258..9283
                     /locus_tag="ACG8MY_000983"
     CDS             8258..9283
                     /locus_tag="ACG8MY_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0430203.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9280..11463
                     /locus_tag="ACG8MY_000984"
     CDS             9280..11463
                     /locus_tag="ACG8MY_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0430204.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11463..12593
                     /locus_tag="ACG8MY_000985"
     CDS             11463..12593
                     /locus_tag="ACG8MY_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0430205.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12590..13267
                     /gene="pgmB"
                     /locus_tag="ACG8MY_000986"
     CDS             12590..13267
                     /gene="pgmB"
                     /locus_tag="ACG8MY_000986"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0430206.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13351..13878
                     /gene="msrA"
                     /locus_tag="ACG8MY_000987"
     CDS             13351..13878
                     /gene="msrA"
                     /locus_tag="ACG8MY_000987"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0430207.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14079..14765)
                     /locus_tag="ACG8MY_000988"
     CDS             complement(14079..14765)
                     /locus_tag="ACG8MY_000988"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0430208.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14845..16383
                     /locus_tag="ACG8MY_000989"
     CDS             14845..16383
                     /locus_tag="ACG8MY_000989"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0430209.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16503..16970
                     /locus_tag="ACG8MY_000990"
     CDS             16503..16970
                     /locus_tag="ACG8MY_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0430210.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17504..17932
                     /gene="rplM"
                     /locus_tag="ACG8MY_000991"
     CDS             17504..17932
                     /gene="rplM"
                     /locus_tag="ACG8MY_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0430211.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            17948..18340
                     /gene="rpsI"
                     /locus_tag="ACG8MY_000992"
     CDS             17948..18340
                     /gene="rpsI"
                     /locus_tag="ACG8MY_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0430212.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18429..19595)
                     /locus_tag="ACG8MY_000993"
     CDS             complement(18429..19595)
                     /locus_tag="ACG8MY_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0430213.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(19650..>20216)
                     /locus_tag="ACG8MY_000994"
     CDS             complement(19650..>20216)
                     /locus_tag="ACG8MY_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430214.1"
                     /translation="IKFYIDSSKKTIKQISTETGINYTTLTNYYRGERNPRNTETWNI
                     LADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIASLDSDYFTELSNL
                     YFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKALNLNIDERKELE
                     SELFGNLTQVQVNNLKREITARLISKLQ"
BASE COUNT         6479 a         3653 c         4272 g         5812 t
ORIGIN      
        1 aatgtgttta aaaaaacgaa ctataaaaac ttatacacaa cgtggttaaa aatagttttt
       61 tcttccctgt ggaaaaaata gtttaacaca aacattatag ttatccacag ctatctaaaa
      121 cagatggaaa ataagaaaaa ttcgctaaaa taccgggagg gggtaacgag ggatgagtga
      181 ttgaaaaaag aacagagtaa ctgacttatc ctcagtgttt ctgttctttt ataattctgt
      241 tctacatttc ttgttgttgg atcgaatctg tgagagtaat ctcggttgaa agaatgattc
      301 ttttttcttc agggtattgg tggtggatta gttggaataa aagttctgtg gctttgattc
      361 ctaactgatt tgtcggaatg tctatccgag tcaactgtgg cgaaaatagg gaggacaata
      421 agggatcact gaatcctgcg atcgaaagat catcaggtat ctgctttcca gattgttctg
      481 ccactttgta aatactcaat gattttaatg catcggtaga aaaaatcgca gttggtcttt
      541 ctcgtagctt cattaaatca ttcattgctg ccatgctatc ttctaagcga aagccgccgt
      601 ctataatcca ttcattacgg atatctaggt ggtgatcaaa taagcattga cggtaaccat
      661 tgagacgatc attggataca taaacttctg tcggagcatg gagacagccg atatgacggt
      721 gtccattctt gatcagtgtt tccaccaaat cgtaactatc ctggaaatta tccgtgtcca
      781 ctgagtagat gttcgtgtag tcgtttccga cttttccgat taccacaata ggaatattgt
      841 attgatccaa ttcaaaaaga agtgtttcat ccatcgtaga gcttaaaata ataattcctc
      901 ggatcatttt ttcttggatc tttttcgcgc atttttctag ctcttcttct ggtgtttttg
      961 ccgtttgtag aatcaagtcg aagttatttt gttcagaaac ggcagaaatg gattgaataa
     1021 catctgaaaa aaaaggattg ccataggtcg tatgagaagt acgatgagat aatgctagaa
     1081 tcgcgtcaaa accatttcct gacagtcctc ttgcaatttt gctcggttca taattcaatt
     1141 ctttgattgc ttgaagtaca cttgcttttg ctttatcaga gatatttggc tggttattta
     1201 atactctgga gacagttgat tttgagacat tagctttttt cgcaatgtca taaatagtag
     1261 gtttcaattt tatatgcacc tcctttttgg gagcgtttta gaataattcc atagtacaat
     1321 agacattctt ttttgtctat gattttgaaa atttaggaat agcttgcatg atcatgagag
     1381 accatatttt tttaagaagc tattaattat tgatacataa gctttcttat ttgtattttt
     1441 gtgtatatca aaattattta aaaaaatgct attgacagta agcgttttcc ataatatgat
     1501 caaatcgttg ggaacgctcc caaaaaagaa aggggaatcc gatgtataca aaacttttac
     1561 aagcgattta tcgcgactca gatttgtctg agaaaaccgc ctctatgatt gaacaaaaag
     1621 tcagtgttta tcagcagaag aaaggccagg caggcaaact gcattgggac gaagaagata
     1681 ttttcttgat tacttatggg gatcaatttt ttgaagagca gcaaccaact ttgcgtacat
     1741 ttaagaaatt ttatcaaaag tatttggcag atacatttag agttgtacat ttcttgccat
     1801 ttttcccata tacatctgat gatggtttct ccgttgtaaa ttatcaggca atcaacgacc
     1861 gtttgggcac atgggaagat atagaagaaa tgagaagcga aacacggtta atgttcgact
     1921 ttgtctgcaa tcatatgtct gccgaaagta cttggtttca ggaatattta aacttgaatg
     1981 atacgtacaa agattttttt attgatttgc cactggatac agatacaagc agcgtgacta
     2041 gaccaaggac cagcccgtta cttacaacgt tcattgacaa aaacgggaaa gaaaaacacg
     2101 tatggacgac ttttagcgaa gatcaagtag acttgaattt taaaaatcca gaagttttat
     2161 tgaaaatgat cgatattctt ttgttttact tagaaaaagg cgcttcattt attcgcttgg
     2221 acgctgtggg atttttatgg aaagaactag gaacttcttg tatacacctg ccagaaacgc
     2281 ataaaatcat ccaattattc cgtgcaatcg ctgatgaagc agctccagga acggtattga
     2341 tcactgagac aaatgtgcca cacaaagata atatcagtta ttttgggaat ggcgagaatg
     2401 aagcacagat ggtctatcag ttttcgctac cacctttgac ccttcaagcg attcgttcgg
     2461 gtaatacaag taagttgaaa gaatggttaa aaaccatcga tttcgcatcg aaaaaaacaa
     2521 ctttttttaa ttttttagcc tcccatgacg gtatcggatt gaatccggta cgaggtattc
     2581 tgccggaaaa aacgatcctt gaattagtgg aacagctgaa gaaagagggt gcccgagtaa
     2641 actacaagaa aaacccagat ggaacgacca gtccatatga gataaacgct acgtatctag
     2701 atgcgttaag ttctccaaca gatacgacgg aatggagaag caaacgcttt ttagctgctc
     2761 attcattttt ggtaacgtta ccaggcgtgc cggcaattta tattcaaagt gtgctgggaa
     2821 gtcgaaatga ttacaaaggc gtagaaaaaa caggagagaa tcgaaaaatc aatcggcaaa
     2881 aatttttgtt agcagatatt gagaaagagt tgcagacaga tccattaaga acagcagtat
     2941 atggtgaatt gacccagatg ctatctattc gaaaaaaaga aaaagccttc catccgaata
     3001 gtttgattta cttactggac tcagcagatt cttttatcag ttttattcgc gaagcgaagg
     3061 aagagaaagt cttagttatc cataattttt ccaatcaatc agcaggttat cttttaccaa
     3121 aaggagatta cctagacctg ttttctggac aaattatcca ttgcagtcaa gaacgaaagt
     3181 tgatattcca gccgtatgaa tatatgtggc tgaaaataat aaaataagag gtggaaaaaa
     3241 atgaaaaaaa ttattgttac tacctgtctg ctagctactg gatttcttct tactgcttgt
     3301 ggtgaaagtc aaagctctaa cgaatcacaa cagacaacga ttcaattcat gcactcttct
     3361 gtcgaacagg aacgattaag tgtgatcaat gaactggttg ctgattttga aaaagaaaat
     3421 ccagacatca aaatcaagca agtgccggta gaagaatcag cgttcaatac aaaaatcgtt
     3481 acgttagctc gttccggtaa attaccagaa gtaatggaag tcagtcagga ttttgctaaa
     3541 gtgatggata aagatgaact gattgatcag acggcagtcc aaaacgttat cgaggaagct
     3601 ggagaagata attattatga cggagccaaa aaccttgttc gttcagaaga tggaaaaagc
     3661 tatattgctg caccaattag cggctgggtt caaggtattt ggtatgacaa agaaaaatta
     3721 tcagaagctg gtttttcaga accagaaaat tgggatgata ttttaaaaat tgctcaacat
     3781 ttcacagata aagagaataa acaatatgga attgctatgc ctactgctga aggaacaatg
     3841 agtgagcaag cgttctctca atttgctttg tcaaatggag caaatgtttt ggatgatgaa
     3901 ggcgaggtaa cgatcgatac ggaaaagatg agagaagcat tgtcgtttta tcaaaactta
     3961 tcccaataca cgatgccagg ctctaatgat gtgacagaaa tcaaagatgc tttcatgaat
     4021 ggaacagcac caatggcaat ctattcaact tacattttgc cttctgttta tgaagcgggg
     4081 aactcagaaa atatcggttt tgctatccca acacaaaaag atcaagctgt ctatggcaca
     4141 gtatctggat tgacgatttc ttctggtcta gacgagaaac aaaaagaggc tgctgaagca
     4201 ttcacagctt tcttatctca accgaaaaac atggaaaagt gggtcttgat gtctcctggt
     4261 ggtgctcaac cagtgaataa acaagtagtc gaactggatg gttacaaaga aaatgaagtc
     4321 atcaagtctt ttggcgagct gccatctgag attgctagtg cttttgatga agtacaagtc
     4381 tttggactag tcggggaaaa gaatttcacg aaaatgggag atatcacaag ctcaggtgtg
     4441 atcggaaaag cagtgaacca agtaactgtc ggaaacgaag atgtcgatca agctttagct
     4501 gacgcacaaa aaaatacgaa ataacaagaa gttctgccaa tggtgttcga aaaaagtaga
     4561 tagctattgg cagattatca gggggaggtt tcaatgaatc gtccaaggaa acattctgac
     4621 ttaaaattag ccatgggact tttagcacca agcatgcttt tattagctgt tttagtgctg
     4681 attccaatgc tttccaatat tcagatcagt tttttcgata tgccgctgaa tcctaactca
     4741 gacgccgttt ttatcggatt aagcaattat cttgaaattt taagtgatag tgaattttat
     4801 cgttctttag gtttaacagt tgcttacaca ttcttagttg tagcaggcag tacagcttta
     4861 ggattagccg tagctatttt gcttaaccgc aagttcagat ttaggaaaac ggtgcgttca
     4921 ctgattttat taagttatgt tacgccatct atttctttga tttttgcttg gaaatatatg
     4981 ttcaataata gttatggaat cgtcaatgat ctgttagtag ataggttgca gcttttcaac
     5041 gaagcaccgc tgtggttcga ccagccagtt gctagcttct ttatggtcgt tttgtttgct
     5101 atctggagat actatccgta tgcgtttatt tcatttttgg ctattttgca aacagtcgac
     5161 aaaacgcttt atgaagcagc tgaaatggac ggtgcaaatg cttggcagaa gttcaagatc
     5221 gtgacactgc cggcaatcaa accagtacta gcaactgtga ttgttttacg atctatctgg
     5281 atgttctata tgtttacaga tgtctatcta ctgacgaata aagtcaatat cttaggcgtt
     5341 tatttgtatc agacagcctt tgcgttcaat gatcttggaa aagcagcagc tatatcgatt
     5401 ctattgttca tcattattgc agcggtcgtc ttgctctcac gaaagaaggt ggacttgaaa
     5461 tgaaaaataa aaagagcagg aaaatcttat tttacgcgac gattttgttg ctattagctg
     5521 tcaatttgtt tccatttttg atcatgctga tgacatcatt caaggattca caagaagcaa
     5581 ttgcaacgac acaaactatt tttccgaaag aatggacttt tgaacattat caggatattt
     5641 tcaatgctga gatttttcca ttcatcatgt atttcaaaaa tagtttagtg atctccttga
     5701 tagcctccgt aactgcagta gtgattggga tcagcggggc atacgctttg tcaaaactac
     5761 gcttcaaagc tcgctcgaaa atcaatgcta gcttttacac cgtttatatg ttttccggta
     5821 ttttgatcgt tgttccatta tttaaaatca tttccggtct aggtttgtat gataccaata
     5881 cagcactgat tatcacgatg gtcgttcaaa cacttcctac tgcgatattc atgctaaaaa
     5941 gttattttga cacgattccg actgatattg aagaagcagc aatgatcgat ggtcttagcc
     6001 ggatccagat cattattcgg atcattatac cattggctat ttctggaatc atttctgtct
     6061 tcgtttattg ctttatggtt tcgtggaatg attatttatt tgcttcgatt ttcctgtcga
     6121 cctccgaaaa atttacttta ccaatcggtt tgaacacatt attcagtaca ccagactaca
     6181 tttggggacg aatgatggcc gcttcattgg tgaccgcttt accagtcatc attatgtatg
     6241 cgttatctga acgatttatt aaaggtgatt tagtcgaagg cggcgtaaaa ggataaaaag
     6301 aaaagaaaga ggaaagaaaa gatgaaaaaa ttagttgcat tagaaccaag aatagccgga
     6361 ttcaaagatt acgaagaccg tgagatcgaa aagaacgaag tacgtatccg agtaattttc
     6421 gctactccaa aaccaggaac agaaattgtc gactttagag gattaagtcc atttatcgat
     6481 gaagaatttt cagaagaatg gcgcctattc ttgccaagag aagaaggaag ccaacgcggt
     6541 gtggtatttg gcgaatttca attaggaaat atgattgtcg gtacgatcga agaaacgggc
     6601 agtttggtga cagagtatca aaaaggagag ttagtctgca cttatggacc cattcgtgaa
     6661 acgctgatta caaacggtgt tgataattat aaactacgta aacttgatcg gttggatcaa
     6721 tggaaaaatg cagtatgtta cgatcctgct caattcgctt taggtggtat ccgggaagca
     6781 catgttcgag caggtgatgc tgtagcaatc ttcggtttag gcgctatcgg gcaaattgcg
     6841 attcaattag ctaaaaaagc aggtgctact accatcgttg ggatcgatcc catagcgcag
     6901 cgtagagaat tagcagaaaa acacggtgcc gattacacgc tagatcctat gaaagcagat
     6961 gtcggttttc gtctaaagga attgacagat aaacgtggag tagattcagt tattgaaacg
     7021 agtggcagtg ctgccgcatt acaagcttct ttaataggga tcggctatgg cgggatcatt
     7081 tcttatgttg cctttgctaa accttttcca gctgggttta atcttggtcg cgaagcacat
     7141 ttcaataatg caaaaatcgt ctttgctcga gcatctagtg aaccaaaccc agattaccca
     7201 agatggaacc gtaaacgaat cgaggacaca tgttgggaat tattgatgaa cggttacttg
     7261 gattgtgaag aaatcattga tccagttgtg ccatttgcag aaagcgcaga agcttacatg
     7321 cgttatgtag accagcaccc agaagaaagt atcaaaatgg gcgttcaagt ccgaggagga
     7381 gaacaatgaa attcggtacg caagatcaag catttttccc aagagacatt ttagaaaaat
     7441 ttagatttat caaagactgt ggatttgaag aatacgaaat cgacggtaag ttgttaatag
     7501 aaaatatcga cacagtcaaa caggcaatca aagaaacagg gatcccagta gtgacagctt
     7561 gtaacggcta cgatgggtgg atcggtgact ttatcgaaga acggcgtctt aatggtgtac
     7621 gtcaaattgc tgagattttg cgtgcactaa gcgaggttgg aggcaaagga atcgttatcc
     7681 cagcagcttg gggaatgttc acttatcgat taccgccgat ggtttcaccc cgaagcaaag
     7741 aaggagacta taaagcagtc agcgaatcac ttcgttattt agataaagta gccgaagaaa
     7801 caggtacggt tatttatttg gagcctttga accgatacca agaccatatg atcaatttgt
     7861 tagcggatgc gcgtcattac attgactcta atcaattgaa acatgtgcag atcatcgctg
     7921 atttttatca tatgaatatt gaagaagatg atatttcaaa agcattgact acgcaaaaag
     7981 agtatgtggg tcatatccat ttagcagata atcatcgcta ccaaccagga tctggcgcaa
     8041 ttgattttac tcgccatttt caaacgctac ttcaaaatga gtatgaaggt tatatggtat
     8101 atgaatgtcg tttgcgagga gaaaatgaag tgaaaattta tcaggaatca ttgcaatact
     8161 tgaaaaactg tctggaaaca gcgcgtcatt taaatgattt agagaaagaa tagagaaaga
     8221 ataacaataa gtaaataagc aaaggagaga gaaataaatg aagaaagtag caatcatagg
     8281 cgctggacaa gtagcagaaa aagtacatgc tgcttattat caaaaaagat cagatgtctt
     8341 agaattggcc gctgtggttg atccagtcag ccagcgagca gaagactttt gccgacgtac
     8401 aggattttct catgcgtata catcagttac acaaatgctg aatgagattt ctcccgatat
     8461 cgtgagtgtc tgtacaccca atcgctttca cttcgaacat gtattggctg ccctcgaagc
     8521 taatgcttca gtgatgtgcg agaaaccacc agctatgaca gctgatcaag caagaaagat
     8581 ggcagagaca gccgataaaa acgggcagat tttggcttat ggattccagc atcgtttttc
     8641 tgacgaagca acaatgatca agaatcatct tgatgttctc ggggatattt attacgtaga
     8701 agcgaatgcg cttcgtcggt ctggtgttcc aggctgggga acgtttatcg acaaagaact
     8761 tcaaggaggc gggcctttga tcgattatgg cattcacatg cttgatacag cgatgtatat
     8821 tactggattt cctaaagtaa aaagcgttcg ggcgtacagc ttccaaaaaa ttggtacgaa
     8881 aaaaaattca gggaaattcg ggaaatggga tccaagcaaa ttcacagtag aagatgcaat
     8941 ttttggcaca ctggaattag aaaacggcaa gatcatccaa ctgaatactt cgtttgcttt
     9001 gaatatcaga gaagacaaac tgaatgtggc tttttgtggc gatcaggcag gtgctaatct
     9061 gtatccaggc gaactattcg aagatgtaga tggaaagctg gtttccttac aaaagaccac
     9121 gccagttaat gacccacatg aactaagtat ggctcatttt gtgaatcggg cgctcggtga
     9181 ccagactcag atgattgcag atggctggca aggatatgtc atccagaatc tggtggaaaa
     9241 tttatatcgt tcggcagaaa caggagaagt tattacctta tgaaagtaga agaaaaacag
     9301 tttgatttag agtatatcaa taagtatgcg agtcagatga ccattggaaa tggatttttt
     9361 ggtatcagag gaagccagga ggaagactat cggaatcaag taaggggaat gtttgcagct
     9421 ggtgtatata accgaccttt aggagcagaa tcagccgagt tagtgaatct cccagatgtc
     9481 atcagatggg aaataaaaat cgacggtgaa catttttctc ttgagacgat gaaagttctt
     9541 gcatacgagc gggcactaga tttagaaatg ggagagctta aacgctatgc gctcattgaa
     9601 actgctcaag gaaaaaaagt ggaagtcatt acttgtcgtg ttgctgatca aaataatctc
     9661 catcaggcag ccctgaaagt ggtcatcaag ccgttgaccg aagactgcca agtagatgtg
     9721 aaaacgggta tcgatggaca gcaaaccaat tttggtacac agcagttggt tgaaaaagaa
     9781 ctgcgagtat ttggtgacga tttgatcagt gcatcctacc agacaacaga aagtaaaatt
     9841 gatattggga ttgcagctaa attcaataaa gcaggtgtat tccaagcaaa aaatcgccgg
     9901 atcaccgctt ccttttcaga aaagatcaga aaaaatacgt cctttacgct ggagaagatc
     9961 atcgtgctct ctacctctct tcagcaaaca gacccgttta agaaaagtat tgcccaagcc
    10021 cagaaaagcg ttagttatac agaaatttta gagcagtcga tgtcttattg gacagatttt
    10081 tggcatcagc atcggataac gatccaaggc gattcttctt tcgatcagtt agcgatggac
    10141 ttcgctgctt accatttaca gattatgact ccccgcaatg atccaagtgt tagtgtaggg
    10201 gcgaagggac taactggcga aggttataaa gggcatgttt tctgggatac ggaaattttt
    10261 atcctgcctt tctttttata tcagtttcct gaagtagcaa agaatttgct gctgtaccgt
    10321 tatgatcgat tagaaggcgc acggcaaaaa gcaagagatt atcactatca aggtgcactt
    10381 ttcccatggg aaagcgcaca cactggtcag gaagagacac ccaaatttgc tgcattgaat
    10441 atcaaaaccg gaacaagaca agaagttgct tcagctgccg cagaacatca tatcagtgca
    10501 gaccttgctt atgccgttca agagtatgtc caagcaacag aggatcaatc gtttttagaa
    10561 acaaagggaa aaacgctcat tcatgaaaca gcagcttttt ggctgagcag agctgtaaaa
    10621 gtcgatgatc atttagagat acacagagtc attggtccag atgaatacac agaatatatc
    10681 gacaataatg catatacgaa ctatctggcg cattataatg tggccttagc tgttaaatat
    10741 caagctggcg atccgctttt tttgaaagaa tgtcaaaatt ttttagataa gttatatttg
    10801 ccagtgccta acaaagaaca gctgattccg caagatgaca cgtatctcag taagccagtt
    10861 attgatttga ccacttacaa agaacgtcct ggcagccagt ctattttact tgattatgcc
    10921 cgtagcgaag taaatgaact ccaagtcagc aagcaagcag atcttgtcat gctgttatac
    10981 ttattacctg agtatttttc tcaaccaatc gtttctaaga atttgcatta ctatgaaaac
    11041 aaaacaatcc acgactcatc gttgagtaaa gcgattcacg ccatagaggc ttgccgtatc
    11101 caagaaatgg aatgggcgta tcatttattc caagaagcgt gcctgattga tttaggacca
    11161 gatccgcatt catcagatga cggaattcat gctgcttcat taggagcact atggctagca
    11221 gttatatttg gtttttcagg tattacagta acgaatggaa ctctttctat ttctccaaga
    11281 cttccttcag catgggaaaa aatttctttt ccttttattt ggaaaaatca caggttgcta
    11341 tttgttttga caaaaaaaca aatagtaata aaaaaagaga cgaaagaaac attacctatt
    11401 tttgtcaatg gagaccaata ccagctggaa aaagaactga tcttggaacg gaggggaaaa
    11461 taatgcgtat tctgattgcg ccagattctt tcaaagaaag tctgacagcc ttagaggcag
    11521 cacaagccat tcaatcaggt tttcaagaag tttttgttca ggctgaatat gatctgctac
    11581 ctataggcga tggaggagaa ggaacggttt catcattagc agacagttta aggttgaagc
    11641 ggcaaacagc agtcgttact aatgcatttg gcaaaaacaa agaaatccag tacgcttcag
    11701 acggtcaaac tgcgattttt gaaatggcag aaatcgtcgg attgaaagat gtcccactaa
    11761 accaacaaca gccacttagg ttatccacgc gaggagtagg agaactgata cgacaattgt
    11821 tgcaacaagg tgttcgacac atcatcatcg gcgttggcgg aagtagtacc aacgatggcg
    11881 gcatcggcat ggcagaaggt ctaggttatg ctttttacga tcaagataat caaaaagtag
    11941 aagcactggg agaaaatcta ggtaaaattg ctacagttga taaaacgaac gtgcctattg
    12001 agctgattaa agccaaaatc actgtcatta ctgatgttac taatctttta tgcggaaaag
    12061 aaggcgcttc ttttgttttc ggaccacaaa aaggcttgaa agaaaatgaa ttagcagtgg
    12121 ctgaccaagc catgcatgat ttttatatga gatttgcccc tcaagtaatc gaggcatcgg
    12181 gcagtagtgc tggtggtgga atgggtgcag gactgttagc atttgcttca gggaaaatag
    12241 tctctggcat cgactacgta ttggatttac tggagttcga taaacgggta cagcaagcag
    12301 acctcgtaat cgtaggcgaa ggacgtctag atggacaaag tctaaaagga aaagcgccag
    12361 ttgggattgc tagaagagta ccagagggcg ttcctgtgat agctatttgc ggaagtgttg
    12421 gtaaaggaag cgaaaaagca gcagatgttg gcatttcggc cgtttttccg atccttcctg
    12481 cagctgtgcc tttacaagaa gctttacagc aagccactga taatcttcat cgcacagcta
    12541 aaaacatcgc atctttactt caagtcgttc attttcaagg aggtaatcga tgaaaatcaa
    12601 agcagcacta ttcgatttag atggcgttct tgtagacaca gctcgttatc attacgaagc
    12661 gtggctggtt ttagctaacc agctgtccat tccattcact gagaaagaaa acgagcaact
    12721 gaaaggaata tccagaaccg agtcactaga gcgattgctg tcatttggaa aaatggaaca
    12781 gaaattctca gaaaaagaga aatcagcctt tgctgagcaa aaaaacaatc tatatcttca
    12841 agcgattcaa aaaatggatg aaacatccgt tttgcctggt gcaatagctg ttcttgagta
    12901 cttgaaaaaa acaaacatca agatcggatt gggatcagca agcaaaaatg cacgcctcat
    12961 tttagaaaaa acgaatttga caagttattt tgatgtcctt attgacggca cgcaagtatc
    13021 aaaagcaaaa cctgatccag aagtttttct caaaggagca cagcaattga atgtaccgcc
    13081 aaatgcatgc ttggtcattg aagattcaga agctggttgt caagcagctt tggctggtaa
    13141 catgcatgta ttgggaatag gtgaaaacat aaatcttcct tctgctgaat atgtgattcc
    13201 tgatttaacc gtttttgatc aagtacgttc gttttggcat ttatcagaag ccgtatcacg
    13261 tatataatag tttccgtttt ttctgtcaca agcaaaacgt tatcctcctt ttggaaatca
    13321 aggtatgatg agaaaaaagg gggattttat atgacaaaaa aagcagtttt cgcaggcgga
    13381 tgcttttggt gtatggtcaa accatttgaa gagcagccag ggatcatttc tgtgacatcc
    13441 ggttatactg gcgggcatgt accaaatcca acttatgaac aagtaacaac aggcacaaca
    13501 gggcatacag aagcagtaga gatcgaatat gatccagaag ttatttctta tgatcagttg
    13561 gtggaaatct attggcagca aaccgatccg actgacgcat taggacagtt cgctgatcgc
    13621 ggtgattctt atcgtccagt gatctactat aatgatgaag aagaacgaaa tattgcagag
    13681 cgttcaaaac aagcattgca agacagcggg cgattcgata agccaatcgt gacaaaaatc
    13741 gaaccaagaa gcgaatttta tcctgcagaa gattaccatc aggattatta caagaaaaac
    13801 aagctccatt acaatcttta tcgagaaggt tctggacgtg caggtttcct tcgtcgacat
    13861 tggggcgtca agaaataaga agccgaaaaa gctacttatg gaacaccgta tatcagaggt
    13921 tgtaaaacca gcattttgag ctaatactga gggtcagctg attgaacacc atttagtgga
    13981 gaggttgtga acctgtcgcc tagctccaag tcacaaggat ttggtaagag aacactatat
    14041 atcagaggtt gtgacaagtc gtttgtcaca accttttttc aatacctaag atgaaggaca
    14101 atcagcaacg cactgctcaa aaaaggaatg aaaggcagct tttccaactt ttttttatga
    14161 cagataaagt aaacagaaaa aacggatatt ccaaacagac tagcaagaaa caagatctga
    14221 agcaactctt ctagagacaa gccaccactc caagaaagca tcaacagagt atcgccagct
    14281 ccgagatagg attgaccata gcgaaggcac acaccgccta cgataaacgt taacagcaca
    14341 ctttgccatt ggaagacgcc ggcctgcaac caaaaaatcc ataaaattgc ccaagaacag
    14401 aatagcgtgt gagaatggac catgaagtaa aataaatcca tcaacgaaaa tagcaaagcc
    14461 gaaagcatcc atatgaacgt cgaaaaattt cttgcggaag aaaaagtaaa gaaccaggca
    14521 aaaagaccac cacataaggc ttctgccaac aaatagtaga agggcaaccg acacctgcaa
    14581 tagcggcagc gaaatcgttg gacgatgatc gagagaatcg gaatcaattc ataccaagaa
    14641 agcgaacgac agcatttgac acaatgagat ctaggataaa taatagagtg acctaacgga
    14701 atccgctggg cggttaaaca taaaaatgat ccgatacagc aaccaatgat aaaataaagt
    14761 aacacgccat ctcccccttt tcaaaaccaa gatacgcaag cttaaactaa atttttgtac
    14821 atacataaaa tttgaggtga aaaaatgaac gtttcaatcg gattagatat cggaaccaca
    14881 caaaccaaag cagttgcttt cacagatgaa gcccaagaag cagcatccgc ttattttcgc
    14941 taccctttga tccaagaaac acaagggatg gcagaacaag atcaggaatt gatctttcaa
    15001 ggtgtctgca ccgttataaa agaagtaatt aaacagttgc cttcagacac aatcattcaa
    15061 tttgtctcct tctcctctgc gatgcatggc ctgatattgt tggatgaaga gaaaacaccg
    15121 ttatctcgaa tgatcacttg ggcagataac cgagcagcgg acgaagcaga aaaactgaaa
    15181 tataccgtgc ttggcaaaga attttatcgg cagactggga cgccgattca tccaatgaca
    15241 ccattgaaca aaatcaagtg gctgcaaaac agccaacctg aattattcaa aaaaactgct
    15301 tattttgtcg gtatcaaaga ctatatcttt tatagattgt ttggcgaact gatcagcgac
    15361 tacggaacag cttcaggaac tggatatttt gatatccatg aattccaatg ggcagaagaa
    15421 gtgcttgact acttgaacat tcgaatagag caattgccgc aagtctttcc ttccacctat
    15481 caagtaacag gtctaccatc aaaaacagca gcagaactag gtttgccaaa tgatatatca
    15541 tttgttttgg gaggggcaga cgggccgctt tctaatctag ggttaggtgc ttttggtgat
    15601 acgatcgccg ctttgaccgt aggaacgagc ggagcacttc gttttattac tactcagcct
    15661 cagttccatc cagaaatgga gacgttttgt tatgttctgg atcagaaaca ttgggtagtg
    15721 ggaggagcga ctagcaatgg tgctggcatc tttgattggg cagggcatac atttttacag
    15781 gaagtcgttc aatctgctaa agaaaaagga gaaaatccat atgatgcact gctttcggct
    15841 atcgcttttg tgccagcggg tgccaatggt ctgatttttc atccctattt acttggtgag
    15901 cgagcaccgc tttgggacgc agatgcgttt ggcagtttta tcggtttaca gcggcaacat
    15961 acagaaaaag aaatgatgaa ggcgattcta gaagggatct gtctcaacct ttatcggatt
    16021 ttaaaaggag tttgccagga aaatcagctc acagagatcc gtgctacagg cgggtttgct
    16081 caatctccga tttttcgtca gatgatggct gatgtactgg gttacccgct tgttttccct
    16141 actgtcacag aagcatccgc attaggagct gtgattttag gctggagaag catcggaaag
    16201 atccaaagta tcagtgaagc acaggaattg atagaattga aagagcaagt tggagtacaa
    16261 gaaactgatc ataaaaaata tcagaaaatc tatccattat ttgtttcgac acaaaagcat
    16321 ttagcacaga cttatcagca atttgctcaa ctgcgagaag aattagctga ttctgaaaaa
    16381 tgattggctt tacggataaa aaagcgaact gaaaatcgtt ctcaggtaaa tgattttgtt
    16441 gtacttttca ttgaaagtct actattattt taagtgtaag aaaatgttag gaggcaagaa
    16501 agatgaaatt ccaacaaacc aaagaagtat taaatcaatt agttgcagat ctaagccaaa
    16561 tgtctgttgt tgttcaccaa acacattggt acatgagagg accaggtttc cttacattgc
    16621 acccaatgat ggatgagttc atggatgatt tgaacgctca gttagatgaa gtatccgaac
    16681 gcttgatcac attagacggt tcaccttatt ctacattgcg tgaatttgca gataacacaa
    16741 aaatccctga tgaaattggc cgctgggatc gtactattcc agaacgtttg gaaacacttg
    16801 ttgcaggcta tcgttatcta gcagatcttt accaaaaagg aatcgaagtt tctggagaag
    16861 aaggcgacga tccaacacaa gatatcttta tcgaattcaa aacagccaca gaaaaacgta
    16921 tctggatgat ccaagctcat ttgggcaaag cgccagagat cgacgcgtaa aaactgttgt
    16981 tgaacttgac cgaaaaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
    17041 aacgtcaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga
    17101 ttgaacaccg tttaccaaga ggttgtaagc ctgccgttca gctccgagtc acaagaaaca
    17161 attttaaaaa acagcttctt atgttttatg gatattgtga cttgtgaccg aggagttggc
    17221 aggagaacac cgtttattca aaataagggg ttgtgacaag acttttgtca cagcctcttt
    17281 ttgtttttca ttttgacagg aaacgaccct tttatataaa agaaagaaaa gaagtaaagt
    17341 ttcatataaa tttttcaatc aagcagaaaa acgttgacat tgactagatt agcgggtaat
    17401 atgttaaatg gtattgtttg ccccacaaag agccccggaa actcgaagtg tatgtcaaac
    17461 atcagaactt aaattggagg aaaataacgt gcgtacaaca tatatggcca aaccaggcga
    17521 agtagaacgt aaatggtatg tagtagacgc aactgatgtt ccattgggac gtctttcaac
    17581 agttgttgca tctgtactac gtggtaaaaa taaaccaact ttcacacctc atttggatac
    17641 tggcgatttc gtcatcgtca tcaatgcaga caaagtgaaa ttaacaggta aaaaagcgac
    17701 tgacaaaatt tattaccgtc acagcaacta cccaggagga ttgaaatcaa tcactgctgg
    17761 cgaattgcgt gctaaaaatt ctcgtcgttt gatcgaaact tctgtaaaag gtatgcttcc
    17821 taaaaacact ttaggccgca aacaatttac aaaattgaac gtatacggtg gagcagaaca
    17881 tccacatgca gcacaacaac cagaagtttt agacatcaca aacttaatct aataggaggg
    17941 aatttcattg gcacaagttc aatatatcgg cacaggccgt cgtaaaaacg cagttgctcg
    18001 cgtacgtcta gtaccaggaa ctggcaaaat tactgtaaat aaaaaagatg ttgaagaata
    18061 cattccacat gctgacttgc gtgaagtaat caatcaacct ttcgctgtta ctgaaacaaa
    18121 aggcgcatat gacgtatttg ttaacgtaaa cggcggagga tacgctggac aatcaggagc
    18181 tatccgtcat ggtatcgctc gtgcattgct agaagttgat cctgacttcc gtttagctct
    18241 taaacgtgct ggcttactta ctcgtgacgc acgtatggtc gaacgtaaaa aaccaggtct
    18301 taagaaagca cgtaaagctt cacagttttc aaaacgttaa tcgttgttat accaacgatt
    18361 tcagacactt tctcattgcg agaaggtgtc tttttttcgt tctggtaatg agaaaggtaa
    18421 tgacaatatt ataaaccagt gtaagcaatg aatttttcta acgcatttcg ttgagctttt
    18481 tcagtaacat gagaataagt gttaagggta gtttcagcat ttttatgacc caatcgatct
    18541 tgtgtttctt tgatacttgc tccaccttct agtagaagag tagcgtgagt gtgccggaat
    18601 ccatgaatag taacgtgctt aaatttatat ttcttctcta aacgtttcaa tatattattt
    18661 gaatagtccg catgcaaagg ttgattaatg tcaccgtctg catttacata ggtaaagata
    18721 agttgatttt ttgtatttgt atttatgcca aactgcaaaa gctctcgagc ttgttctttt
    18781 ttccagattt ttaacatttc aatagtttgt gcatcaattg gtaatattcg ttcggaagat
    18841 atattttttg tatttttttg aatcctgaca gtttttcttc ttgcattctt ctcttcattt
    18901 cgaatagttg catagctctt tctaacatac attagctgtt cttcaaaata aatgtcagac
    18961 caatgaagag cagctagctc tcctttacgt ataccagtag taatcaaaag ctggaagatt
    19021 aaaatatgtt ttaaaggctc ttctttattg agaatatccc aaaaagcttt tagttcttct
    19081 aatgaaaagt aattttcatt ttctttcttt tcccgctttg gtagatttgt atttgccata
    19141 ggatttcggt gtaatagctc tgcaaaaatg gcatactcaa atatttgaga gctgtaagac
    19201 ttaagctgtt tgatattctt gaattttaaa gcccattcat tgactgcatt ctgacagtga
    19261 acaggagtaa tcttagaaat ggacttattc ccgaattttg gtagtatatg caagcggaaa
    19321 aggtcttttg tacgttgata tgtcgtttct tgtacacgat tcctataaga aggcaaccat
    19381 tcttcataca ctgttataaa caagtcatta gaaggcttat gatagccggt atcttcaatc
    19441 tcgacctttt ttcttgcttc gtgtaacttg gcttcttttt cagttttaaa cccttttttt
    19501 acatagtatt cttgctttcc attttcatct ttacgaacat acaagcggac actatatcgt
    19561 gcaccgcttt ttgttgtgta cttttgtatt gacataacta atctctccaa tctgataact
    19621 tggcgagttg gctgattgaa taattagttt tattgaagtt ttgatattaa tcgagctgtt
    19681 atctctcttt ttagattgtt aacctgtact tgggttaagt tgccaaataa ctcagattct
    19741 aactcttttc tttcatcgat atttaagttt aaagctttat taataaactt cttttgtttg
    19801 gcaaatagaa tcgtgtattc aaaggagtcc tctagaatac tttttaaaaa aagaaagtcg
    19861 gagtcatctt cttgaagttt gacttcaagt aaagaagtca tggaaaagta tagattagat
    19921 aattcagtaa aataatctga gtcaagactg gcaatattct tgactatttt gtcctttttt
    19981 tctaaacgag cagtttttat tctatcaaga ttaggttgtt ctatggaatt tgtttcgtct
    20041 aatcccatca aataacttac aggaacatgg aaataatcag caaggatatt ccatgtttca
    20101 gtatttctag gatttcgctc accacggtaa tagttcgtaa gagttgtata gttaatacca
    20161 gtttctgtac taatttgttt aatggtcttt ttactggaat caatataaaa ttttat
//
LOCUS       DAZKNO010000037        20057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000037 DAZKNO010000000
VERSION     DAZKNO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.037"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..594
                     /locus_tag="ACG8MY_000995"
     CDS             <1..594
                     /locus_tag="ACG8MY_000995"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFQ0430215.1"
                     /translation="INTNLDVRENKETTKEVQEDLKEYSASLNKVLSESVKKINEIFE
                     DNYKDIGSYLKVFHRILKILNNRYESKDISHEEYKMYVGILRSQIEDYEFYIILINSL
                     FTLRGTGMGIELIETSFFGDELDIDLKQHFEHPEFLTEIIKDNFVEKKSYRESVKTRK
                     KNRKLFENRKINDNLLSLLRIRYTLQDGNKIIISNGV"
     gene            643..1815
                     /locus_tag="ACG8MY_000996"
     CDS             643..1815
                     /locus_tag="ACG8MY_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0430216.1"
                     /translation="MSLFDIFKQSIKNEDPSDWIPDFVFGDEESARAYLKIMAKSTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWAMSDVIYMEYNNEELERFTR
                     GLFEDYAELFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDKIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIADVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1802..2506
                     /locus_tag="ACG8MY_000997"
     CDS             1802..2506
                     /locus_tag="ACG8MY_000997"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0430217.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2511..3671
                     /locus_tag="ACG8MY_000998"
     CDS             2511..3671
                     /locus_tag="ACG8MY_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0430218.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTVEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVEYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3747..4025
                     /locus_tag="ACG8MY_000999"
     CDS             3747..4025
                     /locus_tag="ACG8MY_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFQ0430219.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            4006..4377
                     /locus_tag="ACG8MY_001000"
     CDS             4006..4377
                     /locus_tag="ACG8MY_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFQ0430220.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            4393..4797
                     /locus_tag="ACG8MY_001001"
     CDS             4393..4797
                     /locus_tag="ACG8MY_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430221.1"
                     /translation="MSNVKFEGVQETIQALEKKFGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIDEVVRKNATYRNYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSEPLYFDAVGEEVKKRL"
     gene            4794..5204
                     /locus_tag="ACG8MY_001002"
     CDS             4794..5204
                     /locus_tag="ACG8MY_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430222.1"
                     /translation="MKDMLNIIYEALCLNEYIRSMTYNENTEQYRIKFYEQPETADKT
                     GPMITLRPVDTPNEAYHGSNKELSVEHMVQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFSETKRFVDARRYEGNTKIYDTEY"
     gene            5265..5852
                     /locus_tag="ACG8MY_001003"
     CDS             5265..5852
                     /locus_tag="ACG8MY_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0430223.1"
                     /translation="MTLVGFKKMTIGIFDSTGKIPAANLYVIEGEQDKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITALKEMVFPAAGE"
     gene            5925..6392
                     /locus_tag="ACG8MY_001004"
     CDS             5925..6392
                     /locus_tag="ACG8MY_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFQ0430224.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6438..6746
                     /locus_tag="ACG8MY_001005"
     CDS             6438..6746
                     /locus_tag="ACG8MY_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430225.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6941..13705
                     /locus_tag="ACG8MY_001006"
     CDS             6941..13705
                     /locus_tag="ACG8MY_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0430226.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGITGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRDLATKLPASHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKMLKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKALDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDNTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDIVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPNDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPNAKGTNFHPG
                     GAAMVNDQKGPTYEELITLPSGEAFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYADG
                     IGNITTVSSTLNLDALILAINELTTVLRTQLMAETTNAKDTKLSDETKVADPIIPGSL
                     SEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGVAW
                     NKNLLNGWNSLTGTFISTINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYGS
                     FIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMVG
                     GMGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHEE
                     YVQMPDGRGFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDLL
                     DDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDGA
                     VNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRYT
                     AAANYAFEKFPSKIAYVITNGRVRDRMGLSGTGSSGQWTNWPDGDHFDHIHLNGSMGS
                     GDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGND
                     GLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIGK
                     GHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGDDKKRSNTT
                     NTADNSAELVTLIKQQQKQHNELMMILRAILGKDLSLKSSDIGQAANHYMGSDLNKLR
                     YTNGGV"
     gene            13709..14530
                     /locus_tag="ACG8MY_001007"
     CDS             13709..14530
                     /locus_tag="ACG8MY_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HFQ0430227.1"
                     /translation="MFYKLQFNQNGKLFDPQRKEKIVCKEIKRQAPVYEVNYEDFEGT
                     NGSREANASFRPFELVLTFDIFYKNKHDKELLLTEFYELIFVGYQYYISYDLSPGKRF
                     KVNPTNFEITEEENDYSTIEITFSIPSGSAESSATTLTDFNLEEEWQFSQGLVAEDYK
                     YTHQTSHFIIYNAGSFEIDPREHYLRIALEGESEGNVTIFNKTTGDRFIYYPSLSTNL
                     GQTLVLDGVIPKLNGVSCGINTNHGLINLVEGVNEIEIQNITRVKSSWDFRFLYK"
     gene            14539..15582
                     /locus_tag="ACG8MY_001008"
     CDS             14539..15582
                     /locus_tag="ACG8MY_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0430228.1"
                     /translation="MTELIIRNYEQTKEEILVGYDKGSFYENWQQNETWEIGFTVTSN
                     SLNQEVFDLVEYESSVFYNGQEFVIKEMTRKALGQLLTKQVVATHIYYTVQDGYQYNT
                     VTGAKSISQLLTHVFSAGSRGFTWEVIDPNKKFLTVEQENFGNANYLKLINEILSDYN
                     AVVIPNNKHLTFYPASEYGQQTEEQIRYKYNTDEVSFDIDTYSLKTQIKGYGKLKDGA
                     NTENPKDSDYIFTPITYTSPESQKWGIRIQDPVKDERYTVSGNMLERLKTDLQDYPNI
                     SGSVTLKWKISPNKGDHVPFIYEPLNINTYIQVVGIKTYPAIPNKPPEITLSNTKKTM
                     TSILAEMAKKGVI"
     gene            15582..16634
                     /locus_tag="ACG8MY_001009"
     CDS             15582..16634
                     /locus_tag="ACG8MY_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430229.1"
                     /translation="MGLLKLISNRISTEWKEKFNKNIDYLNDLEKKLSDQDKSMNSRI
                     DNLVLHSGGDSPNEVVDARVNHKGETFATLQGRLTDTEKKVSESVSTLEKNQADIKAQ
                     VAQLNNSVETIIGGSADWINLYVSADLGSDQIGDGSEEKPFATIQMAVNQIPLVSIPG
                     IRIWVDDGVYLEDVFIRNVSATTIHIGPKNDTSVIDPSKSDMPVKLRSLAFYQCKGFF
                     KVTGLQFVDTINAPKNSGLIYSLMLLQGGYLSVDKCKFAEDNRNLTSAAIYTEGLSAS
                     NVYNTCYFYRQNIVVYANLMSQVLISQQTSGKENTTGARSKDAIIRGKFSVGFADKNE
                     DVKDLGLIITKGTVLS"
     gene            16634..18817
                     /locus_tag="ACG8MY_001010"
     CDS             16634..18817
                     /locus_tag="ACG8MY_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0430230.1"
                     /translation="MAFKINESIIVIQAEATSPNRTNVVFWSHDRGTAKLRMKLVRKN
                     GIPQSLPEGTTVPIRLMFRSATAEGGYGKHDYLATIEDRVNGIVSIVLEDNILGYVGI
                     VEGSVYIDFPNDRSLDTAGRFTFDIKRSPIDDSTPELEDYYFNGFSQTIDKIEKILAD
                     GKQEIEQKITESETQIDAKLKNTNDKITKANQDVTTLNTNIDKANDRIDQTNQQISDL
                     GKLKKMYSNSIDFGNYDYSGNPNLLSKLSYDLIENQNTSAGTLSKGENSFKYNKISAE
                     VEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKI
                     VLDLPINSNSITQEWKEFSITGTTPTFSPQAYHPWIQFRAQDGILGEIEMSYDIKIEE
                     GSTATPFQPNLLAEPYNMCREYPNENIADHTVKFPIESGDHQIYQGYTEEELMIGQTY
                     TITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVAMPKLFRVYQY
                     PRSTVGACQIDWLKIEKGNTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYAEKGLN
                     NTVSLTEPETVLGLKNFKDGVQIDGVDVATVKNLGSVLQSGSGLTTIQSDKQSAFDEF
                     SYEFERIGDRVFFHARVKTNSTTAVASHVNLLEIPLGFQFPIGWTIVGVPLSVVQWTT
                     PQGEISALVITDSTGKQVVKFATNRVGNHYISGEWRTADSYPETK"
     gene            18829..19275
                     /locus_tag="ACG8MY_001011"
     CDS             18829..19275
                     /locus_tag="ACG8MY_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430231.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGLRADGEPL
                     TIADQMFDPKLNQWIVLANTLDHNDLNNLKAMYEALEHENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPTLKGGE"
     gene            19277..19414
                     /locus_tag="ACG8MY_001012"
     CDS             19277..19414
                     /locus_tag="ACG8MY_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0430232.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            19449..19691
                     /locus_tag="ACG8MY_001013"
     CDS             19449..19691
                     /locus_tag="ACG8MY_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0430233.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKNNNKWAWGYMIGLGITIIGYFLTKL"
     gene            19707..19904
                     /locus_tag="ACG8MY_001014"
     CDS             19707..19904
                     /locus_tag="ACG8MY_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0430234.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            19915..>20057
                     /locus_tag="ACG8MY_001015"
     CDS             19915..>20057
                     /locus_tag="ACG8MY_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0430235.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQIA
                     NNQ"
BASE COUNT         7127 a         3184 c         4302 g         5444 t
ORIGIN      
        1 ataaatacaa atttagatgt tagagaaaat aaagaaacta ctaaagaagt acaggaggat
       61 ttaaaagaat atagtgcatc attgaataaa gttttaagcg aaagcgttaa aaaaattaat
      121 gaaatatttg aggataacta caaagacata ggtagttatc taaaagtatt ccataggatt
      181 cttaaaatac taaataacag gtatgaatca aaagatattt cgcatgaaga atacaaaatg
      241 tatgttggca tattacgtag tcagattgaa gattacgaat tttatataat tcttataaat
      301 tctttgttta ctcttcgcgg aacgggtatg gggattgagt taattgaaac atcattcttt
      361 ggtgatgaac tagatattga tttaaaacag catttcgaac atccagagtt tttaactgaa
      421 atcattaagg ataattttgt tgaaaagaaa tcgtatcgtg aatcagtaaa aacaagaaaa
      481 aaaaatagaa aactatttga aaatagaaag ataaatgata acttattatc tcttttgaga
      541 ataagataca cgttacaaga tggaaataaa attattataa gtaatggagt atagtcctag
      601 aaatagggct ttttatttta ccttgaaagg tggtgaaaat atgtgagttt atttgatatc
      661 tttaagcaat caataaagaa cgaagatcca tcggattgga ttcctgactt tgtgtttggt
      721 gatgaagaat ctgctagagc atatttgaaa atcatggcta aaagtacagt tcttgatttt
      781 gttgcaagaa ctatgtcaac gttagaagtt aagtttaaaa ataaagatgg gacatcagat
      841 tgggattaca ttctaaatgt ccgacctaat tctgatatgt cagcagctac attttggcaa
      901 aaattttttt atcggctctt agatgacaac gaagtgttag tgatatttac agatgataat
      961 caattgctga ttgctgatga ttttagtcga acagagtatg ccgtgtatga tgatgttttt
     1021 acaaatgtga tggtgaaaaa ttttgtcttt gaacgttcat gggccatgtc tgacgtcatt
     1081 tatatggaat ataataacga agaattagaa aggtttacac gaggtttatt tgaagattac
     1141 gccgagttgt tcggacggat tctagaagtt gcaatgcgca ataaccaaat tcgtgggtct
     1201 gtctccattg attcaacagg aacggcaaat gatgaaaaag gaaaagatgg taagactaga
     1261 tctcaaagat tacaagaata tattgataag atttggaact cttttaaaac taactcagtt
     1321 gcaattgttc ccaaagtgaa aggctttgag tatgaggaat acaccaataa acaaggcgta
     1381 tcgaatcaat cactagatga actgaataag atgaagtcgt ctttaattgc cgatgttgcc
     1441 aacgccatag gagtacctac ggcgcttatt tatggcgaaa aagctgaact agattcaaat
     1501 attaaagctt ttcgtaaatt gtgtattgct ccactagtta aaaaacttca ggacgaattg
     1561 gtggcaaaaa taattagtcg acaagagtac aaaaacgggg aacgtataga agttaaaaaa
     1621 gttctccaaa aagatctatt agagtatgct actcaaattg acaaaataac atctggtggt
     1681 gcgttttata ttgacgaagt tcgtgatgaa gttgattatg atgagttgcc aaataatgaa
     1741 ggaaaaattc gtattcgtac gaaaaactat gaagaaactg tgaagggagg tgagaacgac
     1801 aatgccgaaa gctaaaaaag taccatttca gtttactaac gaaattcaga atggtaagca
     1861 cattctcacc ttgagtggaa atgtacagaa aaaatattgg cgtgatgatg atgtcataaa
     1921 cgctaaagat attcgtgact ctctagatac tgtaaccgat gatattgtaa tcaagttgaa
     1981 cagcccaggc ggtgatgtgt ttgaagggat tgagatttac aattatttaa aagatcaccc
     2041 atcaaatatt actgtagaag ttacagggct tgccgcaagc gcagcaacgt ttattgtcgc
     2101 tggtgccaat gatgtgatta tgaatgttgg gacttctttg atgattcatg aagcttctac
     2161 atttgcttgg gggaataaac aggacatcca aaaaacgtta aacgcattgg aaacgatcga
     2221 tgattctatc ttagctattt attcagataa gactggccaa tctgttgata agttgcgtga
     2281 atggatgaat gaagaaaaat ggtttacagc agatgaagct gtcaaatatg gatttgccaa
     2341 ttctgtgaag cgtgaagaac cacaaaacga acctcaaaat attgcagcga tgattcaaga
     2401 tgctgttgcc gttgcaatag cgaatttaaa ggaacctgtt actaataaaa tcgaacaaga
     2461 atcaaaacaa aaatcattaa tcgcacgatt gcgaaaagga gaataaagat atgttaaaaa
     2521 ttacagataa aactgcagat gcaaaaaagg catttaatgc tatttcttcg aaagaagatg
     2581 caacacccga acaaattaat gctgctctag aagaatatgt aactgccatt gcagaagatg
     2641 ccggcaaaca ggtgcgtgct gaatatgaag aattgaaaaa tgtgacggac aaccgtgtat
     2701 tagaggctcg tggaattcct acactaacag tagaagaaac aaagttttat aatgaagccg
     2761 taaaatctgg aggtttcgat tctgacgtag tttggccaga aacgattttg gaacgtgttt
     2821 ttgaaaatct tcaaaacgat catcctattt taggcctcat taactttaca ccaactgtag
     2881 gtcgtgtcaa agtaattaga gcacgtcgta aaggtgttgc tgtttttgga ccattgcata
     2941 aagaccttga aggccaacta gatgctgaat ttggtgcaac tgaatttgtt caacttgcgt
     3001 tgaccgcatt tttcttaatt tctaatgata ccttagattt aggaccacga tggattgacc
     3061 gttttatcaa tctttcttta agtgaagcag tacgtgatgt ttgggctaaa aaaattatta
     3121 caggttctgg taaagatgag ccaattggat tgcttaaaga tctagatggt gcggtcacaa
     3181 gtggtgtata tccagataaa gctagcgcag ggacattaac ttttgcgaaa gagaagattg
     3241 ttcttgaact tgcaggggtg atgaaaaaat tatctaaata cacatacaaa gtagatggtg
     3301 acgatgaagg tgaagtggaa taccgttctg ttgctggacg agtgtatcta attgttaatc
     3361 ctgttgatta ctgggatatc gtagctagag ttacatttgc aaatttaaac aatgtgtatg
     3421 ggactacgat gccatttatc gctgttgatc atatcatcga atctgtagac gtaccagaaa
     3481 gtaaatgcat tgcatacgtt gataaagagt atgaagctac tcaatcaaga gctgaaaaag
     3541 tctatgtata taaagaaact tttgcaatga aacgcgcaac tttgtatgct gtcgacatgc
     3601 ttggtaatgg ctatccaaca aataatgatg cagcacaagt atatgacttg gactttacag
     3661 cgggggagta acgtcccctg caattggtga gataacaccg acgactgatg gggcagtcat
     3721 taatctttcg taaatggggt tagagtatga acactgaaga atttataaaa gaatataagg
     3781 cccgatttcg tattttccat acttccgaag atgaggatat cgggaaacag ttagagagcg
     3841 ggtttgccga tataaaatct ctaatcggca gttttgatcc ttccacttat gagaaaggaa
     3901 aagaattagt ttatgaacgt actcggtatc tacggaacga atcactagag tacttttacg
     3961 acaattttca aacaatgatt atggatgcat caattgatct ggcaggtgct ggatatgcca
     4021 attaaatcaa attataagaa gcctaaaatt gtagctggtg atctaaatac gccagttact
     4081 ttttttgagt ttcaacctaa taagggtcca gaacctggtg atgaagaaaa aaaggaactg
     4141 tatttttgta cagcactagt ttacaaccca tcaatgaaag atcgtgacat tcttggcaca
     4201 aaagaaggcg ttacgataaa aatacgggat ccccatcaat cttatattcc tactaacaaa
     4261 cataaagtaa ggatattaga ttatcgatat ggagataaag tttgggaaat aatcgatgtc
     4321 tcaccggatt tggaagaaaa ttcaattgta aagatagtat tagaattaat tacttaaatt
     4381 tggtgataaa cgatgagtaa cgtaaagttt gaaggcgttc aagaaacaat ccaagcttta
     4441 gaaaaaaagt ttggagaaaa gaaaactaag acattaacaa aaaaagcaat caatgttggc
     4501 gctgaaaaag tagaaaaaca gttgcaaacg gacatgaccg tttttaaaga caaaggttat
     4561 acgattgatg aggttgtacg taagaacgct acgtatagaa attacaaggc agaagctgaa
     4621 atcggttgga atggtcctca tcagcgttat agaatcattc acttaaatga atggggatat
     4681 acacgaaaag gtaagcaaat taggccaaga ggttttggcg taatcactaa atcgcttaaa
     4741 aattccgagc cactatattt tgatgctgtt ggggaggagg ttaaaaagag attatgaaag
     4801 atatgctcaa tatcatttat gaggctcttt gtttgaatga gtatattcgt tcaatgacat
     4861 ataacgagaa taccgagcaa tatcgtatta aattttatga gcaacctgaa accgccgata
     4921 aaacagggcc aatgatcaca cttcgtcctg tagatacacc taacgaagca tatcacggaa
     4981 gtaacaaaga gctttctgta gagcatatgg tacagattga tgttgaatcg aaatatagag
     5041 caacatgcaa gcagattcaa tatgagataa aaaaagagat gaaaaaacta ggctttggcc
     5101 aaatttctgg tcaagggtta gatgaatact tttctgaaac aaaacgcttt gtagacgcgc
     5161 gtcgttacga aggaaataca aaaatttacg ataccgaata ttagaaatta agacacgaaa
     5221 ataacgtgtc tttttttatt acccaaaatt aggaggaaaa ttttatgact ttagtaggat
     5281 tcaaaaaaat gacaatcggg atctttgaca gcacagggaa gattccagca gcaaatttat
     5341 atgtaattga aggtgaacaa gacaaagggg cgactgtatc tgctgaaatc agtgggctat
     5401 caaaagaacc atcaaaagtt tacggatcga acatcgctta ctatgtctct caaaaaggaa
     5461 caggcgatgt ttcagctact tttggattgt tagatttacc aacagaagta aatgacaaaa
     5521 ttcttggtta caaaaccgat acaaacaaaa tcagcttctt gggagaagat actgagccac
     5581 catattgtgc aattttgatg gaatcggagg atttgaacgg cgatacagca atgcttgcga
     5641 tgttcaaagg aaaattcagt cgtgaatcta ttaatcttaa cactacgaca aatgaagctt
     5701 tcgaaccaga agcagaagaa tatgtatttt cagcgattgc caatgatgtt gaaggtgatg
     5761 ctaaaggcca aacagtcgca aaatatgttg gagacgaaga agcttctatt actgctttga
     5821 aagagatggt atttcctgca gcgggggagt aactagctcc gttgttggag caatcactcc
     5881 cactattaat ggggtaacag ttgaattaag ttaggaggat aactatgcca gatacattta
     5941 aaatctataa aaaaaatggt actgaattta caaaagtcgc agagggtgaa agtccacttt
     6001 ctattactgg aattgcagca aatactcaag ttgctaaagg agactaccaa gctacgcgtg
     6061 tggttggagg tcaagaatca gtaaaagtag atattcctgc atttaccact cttccaattt
     6121 cagttactgg tgtgacgttg gataaaacga cagctgaagt cgaacaaggc ggaaccttaa
     6181 aattaactcc tactgtaaca ccagcaaatg caacagacaa aacaggaagc tggagcagtt
     6241 cgaatacagc tgtagctact gttactggag gcaacgttac agtaaaaaca gacgctgaag
     6301 ttggcggaac tacagaagtt tcatttacta ctacggatgg cggtaaagta gcaaaatgta
     6361 ctattacagt tattgaaaaa gcagcgggct agtcttcgga ctagcttttt tatttggaaa
     6421 ataatggagg aatctaaatg gcaaaagtaa gaattgaatt gaaagaagaa tcaggaaaga
     6481 aagtctatga aaacatagac acaacaggta aagattatcg aaaagcttta gaaacaatca
     6541 aaaagctaaa tgaagatgga gtaatgattt ggaatcgttt agacatttat ttggattttg
     6601 ctgtaggtat cttcagggat agcaaattga cagctgaaca aattcttgat ggcttaccat
     6661 ctgagaaggt aatgagcaca ttggacgaca tcctaggaga agtaatgggg attgaaagta
     6721 atccagaccc agacgcaaaa aagtaacacc cgaagaagca gaagaaatgt acttaaacct
     6781 atgcagagag ttagtgaaag cgggatggtc actatctgac atagaagata attcctttga
     6841 aatgatgatg aaaattgcgt gcacttcgcc taagaaagag aaacaaaaag aagttgatct
     6901 taaagacttc ttgaaatcca tgtagagagg aggtaaacat atggcaaatg gaaaaccttt
     6961 aggcaatatg attataaaac tagacttaga cagttcagct ttttcaaaag gtctagccgg
     7021 agctaaaaac gctgttaacc atcaaatgaa agcaatgaaa tcacagatgc aagtaatgaa
     7081 ctcttcaggg aattctctcg gtgctttaca agcaaaatat aatggtttag gcggagttct
     7141 acaagcaaat gaaaagcaag tagaattgct cactaaggct tataaggata gttttgactc
     7201 taatggaaac gctactgctt cgactgctaa atacgctaat caattgaatc aagcaacagc
     7261 tagatcagca agttacgaat ctcagatgaa aacaaccgtt ggacagattg ctcggatgaa
     7321 agtggaaacg gaagggatta cggggaaact aaaagctaat tctgatgtat tgattaattc
     7381 tgggaaaaaa attgaagcct ttggtaaagg agtttctggg ataggatctg ccctaactgt
     7441 tggagtaaca gctccaatag tggcaggtgc aactgctgtt actagtgcag ctatttcttg
     7501 ggaaagtgct tttgcaggag taaaaaagac aaatgatgaa gttgtagatt cgaatgggaa
     7561 cgtagtttac tcctacagtg atttagaaaa aggccttcgt gatttagcga ctaaattgcc
     7621 agcaagccac caagaaatag cgaacgttgc tgaagcagca ggtcaattag ggattaagac
     7681 agaaaatgta gttagtttca ccaagacaat gattgatatg ggcgaatcta ctaatatgtc
     7741 agcagaaaca gctgcaactt ctttagcacg atttgctaat ataacccaaa tgtctcaaaa
     7801 agactttgat aaattaggat cagtcattgt tgatctaggt aacaattttg ctacaaccga
     7861 atcggaaatc actgaaatgg gactgcggtt agcgggtgct ggtaaacaga ttggaatgag
     7921 ccaaggcgaa attctaggat ttgctactgc tctatcctct gttggtgtcg aagctgaagc
     7981 aggtgggtct gccttttcga aggtcatgat ccagatgcaa ctagctgtag aaaaaggatt
     8041 tggcgctttt gatcagctga aacagatggc agaagaacaa ggtgttccgt gggttaattt
     8101 agtaaatgct gtgagagacg gtggaaaatc gttaaaagca gtatcagagc aaatgggatt
     8161 cacttcatct gatttaaaga aaatgtataa agaagcggac aattcgaaaa gttcgcttga
     8221 aaattttgcg aatgtggcag gtgttacaag tgaacaattc cagaagttgt tcaagagtga
     8281 tccttcagaa gcaatcatca agttcattca aggactgaaa gattcagaaa aacatgggac
     8341 ttcagcaatc aaagttctgg atgacatgga tataaaagaa gttcgactac gcgacagttt
     8401 attgagagct gcaaatgcta gtgacatatt caaaggagca attgaaaaag gaaacggcgc
     8461 gtggaaacaa aatacagctt taacaaatga agctaataaa agatacgaaa caactgaatc
     8521 aaagctaaag atgttgaaaa acgaagtgat tgatacagca atcgatcttg gaggtccatt
     8581 tgtagacgcg ttgcgtgatg gtctaaatgt atcaaaaccg ttgataaaaa acttagggaa
     8641 tatggcgaaa gctttctcag ccatgagtga tgatcaacaa agaaacatta tcaaatgggt
     8701 tggattagca gcagcagctg gtccagcgct gaagctttta ggaactggta cttcagtaat
     8761 aggtaaagtt acttcaacta ctggaagatt gacgaaaagc cttgttgatt tagcagcgaa
     8821 agcagcagaa aagaaggctc tggatgcctt tgcatcgtcg gtaacaacag ctggtgctgc
     8881 agctgcaaag acagcaggag ctggaggtat tggcgggtta gtccctatga tgggaaaaac
     8941 agctagtgct gcaagtactg ctgcgggcgc tagcggaata ggcgcaatga caacatcact
     9001 aggtttgtta ggtcctgctc tacttggtat tgttggcgtt ggtggcgcac tagcagttgg
     9061 ctatggagca tggaaattat ttggtgaaga agcttggaat tcctcgcaaa gagtaaagca
     9121 atggggaaca gatgtcggtg aggaaataga taatactctt gacggagtac agaagaatat
     9181 tgaaggtgca aatggtcaat tcggtcttct caaagagggt tttactgttg atgatgcgaa
     9241 taatatggcg gctaatttcg caaccgctgg aacatctttg gaaacatctt taacaggtag
     9301 aataaaggca ttagacgatg cactaaaagg gctacctcaa tcagttcagg atgccatgaa
     9361 agaagtagct gaatcagaag aagaaacctt agggcaatct ctttctaaaa taaaagacaa
     9421 caatgaagaa ataaaaaaaa tcagagaaag agccgcaaat cagaacagag acttgactgt
     9481 ttcagaacta caacagatac aggatctgat gaaaggaact tctcaagcgt atgtggatac
     9541 gctcgatgtt tctgctaaag acagaaaaaa gatactatca gccatgaatg gtgatgttgc
     9601 acaggctaca gaagaagaag cgaagacgtg gttacaatct ttagcgaaac aaaaagctgc
     9661 aactaccgaa aattatagac aaatgcttga agaaaaaaag gcggctttaa aggaaaaggg
     9721 agtaagcaaa gaattcattg atgcattaga agatgaaatg aatgattaca tcaaaactac
     9781 aaatcaagga ttcgatagcc aaatagctgc tataacaaat aaatatccac aattgattaa
     9841 cgaagtgagt ttatcaaatg gtcaattatt tagcaaaacg gcagctgcta tggacaatac
     9901 tggtcagtat acaaaagttt ttctcgaaag taatgaaaag attttagccc aagcgaagaa
     9961 aacatcggat gaaatagcag ataatgccga aaaaaatgca gaacaacttg gtatggtagc
    10021 tgatgaggct acgcatgctg gatcaacttg gaatggatta gttttagacg aaaaaacagg
    10081 aaaagtaaaa actaacgtac aagaagtagt gaatgaagca acgaaggatt tcactaaatg
    10141 gaatgaactt aaacctgttg ttcatgatgc gaaactaaca acgaacgcaa aagatgtcat
    10201 ttccatagcg gcagtacaaa atggttattg ggatgatatg gaatggaaag aaaaagaagc
    10261 attattagaa gatgagtgtt ctaaaaatgt atacaaggct ttagaaaatg ctggtaaatg
    10321 gaatgagtta gatattcctt ctaaagaagc aatcctaact tcaaatactc ctgaagttat
    10381 gggtgaaact ctcatgaatt tgggactatg ggatacttac aaacttcaag tgaaagattt
    10441 agatttgaat gcgtataaag tatacgatgc tatcgatgga tcagaagaaa aattaaatag
    10501 ttgggctcaa ttagataacc ccacaaaaga attgttatta gataactcag atttttctac
    10561 aaaacttttt aattccgaag aaatgtttaa acgttggaat cagttatcat ctaatgcaaa
    10621 gtttctttca gctaataata ctaaatttac agaggttctt ttatcatctg acagaatgtg
    10681 gaatcaatgg gtaaaccttc ctgataatga gaaaagatta cttgcagata acgaagattt
    10741 agctaccact gttttatctt cagaagaaat atacggaaga tggactcaat taccagataa
    10801 tcaaaaatat atgttagctg acaatactga cttagcaaac aaaatatttg cttcagaaga
    10861 atcatggaat gcatggaaag caattccaga catagttaaa aacttgcttg gtaataatat
    10921 tgatttactg gctaaagtga aagatgggac tgtaagtgta gaagaatata acaaagttat
    10981 gccacttctt aaaactctat atggagataa cgttaattta aaagaggcaa tcagtggggc
    11041 taaaagctct atagagaatt acaacaaaaa tatttttcct aatgataaaa ctgcagtagg
    11101 acacgatgag gcttcacaag ctgctaaaga tgcatttgaa gcgttcaaca tcttccaaac
    11161 aaaaatacca gataaaatta ctaaaactgt atcagctgac tttataggtc ctatgcctaa
    11221 tgctaaagga acgaacttcc atcctggcgg agcagctatg gtaaacgatc aaaaaggacc
    11281 tacttatgaa gagcttataa ccttgccaag cggagaagct tttataccta aaggacgtaa
    11341 tgtcgtttta gacttaccaa gaggttcgaa agtactgaac gccactaaaa ctaaacgtct
    11401 agtacctaaa tatgctgatg gtataggaaa cataacgacg gtttcatcta cgctaaactt
    11461 agatgcttta attttagcta ttaacgagct aacgacggta ttgagaactc aactgatggc
    11521 agaaacgact aatgctaaag atactaaact atcagatgaa acaaaagttg ccgatccgat
    11581 tattccgggc agtctttcag aaaaatcaga tcagtatcta ggaattggag ctcaatggtt
    11641 aaccaatcta atgaatggtt ggaactcagc ggttcctcaa tatatgaata gtgaaatggt
    11701 ttttattaca aactatctca atgcgctaaa acttcaaaac aatcctaatt atacgcaagg
    11761 agtagcatgg aataagaact tactgaatgg atggaatagt ttaactggga catttatttc
    11821 tacgatcaat tcattttgta atcaagcaat ggtcacgctt agaaactaca acactcctat
    11881 gtacaacaat ggccgaacgt ggcagcaaaa taatcttaat ggttggaatt cattgtatgg
    11941 atcattcata gcccgtgtaa atcagcttgg taacgattcg attaacaacc ttcgttctaa
    12001 aagtggtggt ttttacaatg cagggacatt cttgttgcaa tctttaataa acggaatgaa
    12061 ttcgttaggt aattctcttt ctacaacaat gaacaatgtc gctaatacaa tggttggtgg
    12121 tatgggcaaa ggggttaatg gcgtcatatc gggagttaac tatgtgctta aggaagtcga
    12181 gtcaagtaag aatatcggaa actggccaat tccacaatac gctaagggaa ctgatggaca
    12241 tcctggtgga cttgctatga ttaatgatca aaaaggccta gttcacgaag aatatgtaca
    12301 gatgccagat ggacgtggtt ttattgccaa aggtagagac cttcttgtta atttgcctaa
    12361 aggtgctcag gtcttaaatg cttctttaac aaagaaatta aaggaacgtt taaatattcc
    12421 acactacgaa aaaggtattg gcaatttaga tattgttgat ttgttagatg atgaaaaaag
    12481 aatgttagaa ttcttgacta gcaaagttga cttttcaggc attaatgaac catggcttga
    12541 tatgactaaa tcaggaacgt cattaatgtc taaagctgca aatacaatgc ttcaatcaaa
    12601 attaagtgaa ttcttcactc atggcaattt tgatggtgca gtaaatgcca atggtgttta
    12661 tcaatattta gttgatgttg cacagaaagt aatgggtaag ttccccggac ttacagtaac
    12721 ttctggatat cgagcaggag atgcttacta tcacggaaaa cggcaagcta tagacttggc
    12781 ttatccaggt atatctggag atccgagata tacagcagca gctaattacg cttttgaaaa
    12841 attcccttca aaaattgcgt atgtcattac gaatggacgt gtacgtgacc gcatgggatt
    12901 atctggaaca gggtcaagcg gacaatggac gaattggcca gatggcgatc acttcgatca
    12961 tattcattta aacggttcga tggggtctgg cgatatattc aatggaggag ttggaggaag
    13021 tggtgttgca cgctggcgtt cttatgtgtc taaagcattg aaaatgaatg gattaccagc
    13081 tacagcagct tatataaatg cctggatgtc ccaaatccaa acggaatctg gtggtaatga
    13141 gaaagcaatc ggcggcaatg atgggctcgc tgaaggcaat gcaacaggat tacttcaaac
    13201 gaaacccgga acttttgcag ctaacgcttt tcctggtcac ggaaatatta tgaacggatt
    13261 tgataacatg ttagcagcaa tcaattatgc taagaaacgt tacggagttg caggtatgtt
    13321 gcaagttatc gggaaaggac atggatatgc caatggtgga ttgattacca aagacggact
    13381 atatcgagcg ggagaaggaa ataaaccaga aatggttatt ccattgacaa gaaaaacaag
    13441 agctattgaa ttgatgggtc aagcattagc ttttctttct ggcgatgaca agaaacgctc
    13501 caacactaca aatacagctg ataattcagc agagcttgta actttgatta aacagcaaca
    13561 aaaacagcat aatgagttaa tgatgattct tagagcgatt cttggaaaag atttaagtct
    13621 caagtcttct gacattgggc aagctgctaa tcattatatg ggctctgatt tgaataaact
    13681 tcgatatacg aatggaggtg tttgataatt gttttacaaa ttacagttca atcaaaatgg
    13741 gaagttgttt gatccgcaaa gaaaagaaaa aattgtatgt aaagaaatca aacgtcaagc
    13801 accagtgtat gaagtgaatt acgaagattt tgaagggacg aacggtagca gagaagctaa
    13861 tgctagtttt cgtccttttg aattagtgct gacttttgat atcttttaca aaaataagca
    13921 tgataaagaa ctgctattaa cagagttcta tgagctaatt tttgtcggtt atcaatatta
    13981 catttcctat gatttaagcc caggaaaaag atttaaagta aatccaacaa atttcgaaat
    14041 tactgaggaa gagaacgatt attctacgat agaaataacc tttagtattc catcagggag
    14101 tgctgaatcc agcgcaacca ctttaacgga tttcaatcta gaggaagaat ggcaattctc
    14161 gcaaggtcta gtggcggaag attacaagta tacgcatcag actagtcact tcattattta
    14221 caatgctgga agctttgaaa ttgatccacg tgaacattat ctacggatcg cattagaagg
    14281 agaatcagaa ggaaatgtga cgattttcaa taaaacaact ggcgatcgat ttatttacta
    14341 tccatcactt tctacgaatc tcggacagac attagtcttg gatggcgtga ttccaaaatt
    14401 gaacggtgta agttgtggaa tcaatacgaa tcacggtctg atcaatttgg ttgagggagt
    14461 caatgaaatc gagatccaaa atattactcg cgtgaaatct tcatgggatt tccgtttctt
    14521 atataagtag gtgattgagt gactgagtta attattcgaa attatgaaca aaccaaagaa
    14581 gaaatccttg tcggttatga caagggttct ttttatgaaa actggcaaca aaatgaaacg
    14641 tgggagattg gctttaccgt taccagcaat tcgttgaatc aagaagtatt tgatttagtc
    14701 gaatacgagt cttctgtttt ctacaacgga caggaatttg tgatcaaaga aatgactcgc
    14761 aaagcacttg gacaattgct gacgaaacaa gtagttgcga cacatatcta ttacaccgtt
    14821 caagatggtt atcagtacaa cacagtgaca ggtgcgaaat ctattagcca gttactcacg
    14881 catgtattta gtgcagggag tcgcggcttt acatgggaag tcattgatcc aaacaaaaaa
    14941 ttccttaccg ttgaacaaga aaatttcggt aacgcgaatt acttgaagct gatcaatgag
    15001 attctatctg actataatgc agtcgtgatt ccgaataata aacatctaac tttctatcct
    15061 gccagtgagt acggccagca gacggaagaa cagattcgct ataaatacaa tacagatgaa
    15121 gtgtcgtttg atattgatac gtattccttg aagacgcaga tcaaaggcta tggaaaattg
    15181 aaggatggtg caaatactga gaatcctaaa gatagtgact atatatttac tcccatcact
    15241 tatacaagcc ctgaatcaca gaagtgggga atcaggatac aagatcctgt taaagacgaa
    15301 cggtataccg tatcagggaa catgctcgag cggttaaaga cagacttgca agactatcca
    15361 aatatttcgg gatccgtaac cttgaaatgg aaaatcagtc ccaacaaagg cgatcacgtc
    15421 ccattcattt atgaaccttt gaatattaat acgtacattc aagtggtagg aatcaagacg
    15481 tatccagcga taccaaataa gccaccagaa atcacattga gcaacacaaa gaaaacaatg
    15541 acgtcgatac tcgctgaaat ggcgaagaaa ggagtgattt gatggggtta ttaaaattaa
    15601 tcagtaaccg tatctctacg gaatggaaag agaaatttaa taaaaacatt gactacctta
    15661 atgatcttga aaagaaactg tctgatcaag acaaatcaat gaacagtcgt attgataatc
    15721 tcgtgcttca ttcaggaggt gattctccta acgaagtagt ggatgctcgg gtgaatcaca
    15781 aaggcgaaac attcgctacg ttacaaggaa gactgacaga caccgaaaaa aaagtttctg
    15841 aaagtgtctc taccctagaa aaaaatcaag ctgatataaa agcacaagtc gctcaattaa
    15901 acaatagtgt agaaacaatc attggtggta gtgctgactg gataaatctt tatgtttcag
    15961 ctgatttggg aagcgaccaa attggagatg gatcagaaga aaaaccattt gcgacaattc
    16021 agatggctgt aaatcagatc ccgttagttt ctattcctgg aattagaatt tgggtagatg
    16081 atggtgttta tttggaagat gttttcataa gaaatgttag tgctactact attcatattg
    16141 gccctaaaaa tgatacatct gtaattgatc catcaaaatc agatatgcca gtaaaacttc
    16201 gcagtcttgc attttatcaa tgtaaaggtt ttttcaaggt cacaggtctt caatttgtag
    16261 atactataaa tgcacccaaa aatagtggat taatatatag tctgatgttg ctacaaggag
    16321 gatatctttc tgtcgataag tgtaaatttg ctgaggataa cagaaattta accagcgcag
    16381 cgatttatac agaagggttg tctgcttcta acgtctacaa tacttgctat ttttatagac
    16441 aaaatattgt agtttatgcc aatttaatgt cacaagttct tatatcacaa caaacaagcg
    16501 gcaaagaaaa cacaacaggt gctaggagta aggatgcaat cattcgtggg aaattctcag
    16561 tgggttttgc tgataaaaat gaagatgtta aagatcttgg cttaatcatt actaaaggga
    16621 cggtgttgtc ctgatggcgt ttaaaattaa tgaatcgatt attgtaattc aagcagaagc
    16681 cactagtcca aacaggacga atgttgtttt ttggtcgcat gatcgaggaa cagctaagct
    16741 tcgaatgaag ttagttcgga aaaacggcat ccctcaaagc ttacccgaag ggacaactgt
    16801 tccgattcgt ctgatgttca gatctgcaac ggcagaaggt ggttatggaa aacatgacta
    16861 tctcgccacc attgaagatc gtgtaaatgg tattgtgtcc attgtgctgg aagataatat
    16921 tttaggctat gttggaattg tcgaaggtag tgtttatatt gatttcccaa acgaccgctc
    16981 gttagataca gctggtcgtt ttacttttga catcaaacgc agtccaatcg atgatagtac
    17041 accagaattg gaagattatt atttcaatgg tttcagtcag actattgata aaatcgaaaa
    17101 aattctagct gatggaaagc aagagattga acagaaaatt acggaatctg aaacgcagat
    17161 tgatgcgaaa ttaaaaaata caaacgacaa aatcacgaaa gccaatcaag atgtcacaac
    17221 tctcaatacg aatattgata aagcaaatga tcgtattgat caaacgaacc agcaaataag
    17281 tgatctcgga aagctgaaaa agatgtactc caacagcatc gatttcggga actatgatta
    17341 tagtggaaat cctaatttgt taagtaagct atcatacgac ttaattgaaa atcaaaatac
    17401 ttcagctgga acactttcta aaggtgaaaa ctcgtttaaa tataataaga tatcagctga
    17461 agtggaaggt ggagtagagc tatattataa acgaagaggt atagctaact ggctaccatc
    17521 taataaaacg cttgtaatga ctgttaagct tagagctgga gcggactata gtccagttga
    17581 cggaaaactt atactgatta gatataggta tgttgacagt ggaactggca agattgtttt
    17641 agacttacct attaacagta attcgataac tcaggaatgg aaagagttta gtattactgg
    17701 aactactcca acatttagcc cacaagcata ccatccttgg atacaattta gggctcaaga
    17761 tgggatactt ggggaaatag aaatgagcta tgacatcaaa atcgaagaag gctcaacagc
    17821 tacaccattc caacctaact tattagcaga accttacaac atgtgtcgcg aatatcctaa
    17881 cgaaaatatt gccgatcata cagttaagtt cccaatcgaa tctggcgacc accaaatata
    17941 tcaaggttac acagaagaag agcttatgat aggtcaaacg tatactatca cgcttaaagg
    18001 aacaaaaccc gcaagtcaaa cctttgtagc gtataatcat tggactgctc gtttaggaga
    18061 actaaagccg gttgatgggt tgacagacgt atggtctcta acattcacac caacgaatgt
    18121 tgtggcgatg cctaaacttt tccgtgttta tcagtatcca cgatcaacag taggcgcatg
    18181 ccaaattgac tggctcaaga ttgaaaaagg caacacccga acgcccaata ttagtcagtt
    18241 taaatacttc ggtgaaggct tgaaagatag taacaatccg aacgactaca gctgggatat
    18301 cacacctgaa tatgctgaaa aaggcttgaa taatacggtt agtctaactg aacccgaaac
    18361 cgttttagga cttaaaaatt tcaaagatgg ggtccagatc gacggagtag atgtggcaac
    18421 agttaaaaac ttaggtagtg tgttacagag tggatctggt ttgactacta ttcaatcaga
    18481 taagcaaagc gcatttgatg aattttcata cgaattcgaa cgtattggtg atagggtatt
    18541 ctttcacgca cgagtaaaaa caaactcgac aacagccgta gcttcacatg tgaatctact
    18601 agaaattcct ttaggatttc agtttcctat tggatggaca attgtagggg ttccgttgtc
    18661 ggttgtacag tggactacgc cacaaggaga aatcagcgct ttggtaatca ctgattcaac
    18721 tggtaaacaa gtagttaaat ttgcaactaa tcgagtagga aatcattata tttcaggaga
    18781 atggcgaaca gctgatagtt atccagaaac aaagtaggag ggaaataaat gaaaaacatt
    18841 tggaaatacg gacgtactgg cggagagtat gcaggaaaag tattggacga tatgcttgta
    18901 tccgttcctt acacggatca gccaccgctc gaaggattac gtgctgatgg cgaaccgcta
    18961 acgattgctg atcagatgtt tgatcctaaa ctgaaccaat ggattgtttt agcgaacaca
    19021 ctggatcaca acgatttaaa caatctcaaa gcgatgtacg aggcactgga acacgagaac
    19081 ggcgatttaa aacagctcaa cgccaaactc atgctaagcg atgtagcaat taaacaggaa
    19141 aatactgcat tgaaagaaaa agcggatagt ttagcacaaa tcaattcaaa aatgatgctt
    19201 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccaacttta
    19261 aagggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
    19321 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
    19381 taaaatcatt agcaaagaag agagcgcatc ttaagtgatg cgtttttttg ttggaaagtt
    19441 ggtggaacat gaaagaagaa gcgctccaag acgttgtgga gagattagta agaattgaaa
    19501 caaaattaga caactacgaa tcacttagag aaaaggctga tagtgcaaaa gatttagcag
    19561 ataaagccta ttcagtagca ctaaacaatg cagaagacat caaggaaatg aagaacaata
    19621 ataaatgggc ttggggctat atgattggct taggcattac gatcattggc tatttcttga
    19681 ctaaattgta aaggaggtga gaagaaatga ttttaccaga taagtattat caagtcatta
    19741 aatggacagt tttaacagta ttgccagctg catctgcttt agtagccatg ttagggaaag
    19801 cgtatggatg gaatggaaca gatatgatag tgttgactat caatgcagta gcaacatttt
    19861 taggcgttat cactggtgtg tcggcttata atttgaaaaa ataggaggaa acaaatgaaa
    19921 aagaaaatta ctattactgc gatgagccta ttaacggctc tttttttatt gccaattaat
    19981 gggtttgcct atactatcaa caatgaattt aatttgggcg caaatgaagg tagctcacaa
    20041 atagcaaata atcagta
//
LOCUS       DAZKNO010000038        20033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000038 DAZKNO010000000
VERSION     DAZKNO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.038"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..157
                     /locus_tag="ACG8MY_001016"
                     /pseudo
     CDS             20..157
                     /locus_tag="ACG8MY_001016"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            190..513
                     /locus_tag="ACG8MY_001017"
                     /pseudo
     CDS             190..513
                     /locus_tag="ACG8MY_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="ACG8MY_001018"
     CDS             533..1336
                     /locus_tag="ACG8MY_001018"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0430236.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1585..2649)
                     /gene="fni"
                     /locus_tag="ACG8MY_001019"
     CDS             complement(1585..2649)
                     /gene="fni"
                     /locus_tag="ACG8MY_001019"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0430237.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2646..3731)
                     /locus_tag="ACG8MY_001020"
     CDS             complement(2646..3731)
                     /locus_tag="ACG8MY_001020"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0430238.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="ACG8MY_001021"
     CDS             complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="ACG8MY_001021"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0430239.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="ACG8MY_001022"
     CDS             complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="ACG8MY_001022"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0430240.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5974..6633
                     /locus_tag="ACG8MY_001023"
     CDS             5974..6633
                     /locus_tag="ACG8MY_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0430241.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            6718..7623
                     /locus_tag="ACG8MY_001024"
     CDS             6718..7623
                     /locus_tag="ACG8MY_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0430242.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7625..8257)
                     /locus_tag="ACG8MY_001025"
     CDS             complement(7625..8257)
                     /locus_tag="ACG8MY_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0430243.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    8408..8533
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            8577..9101
                     /gene="infC"
                     /locus_tag="ACG8MY_001026"
     CDS             8577..9101
                     /gene="infC"
                     /locus_tag="ACG8MY_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0430244.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9173..9373
                     /gene="rpmI"
                     /locus_tag="ACG8MY_001027"
     CDS             9173..9373
                     /gene="rpmI"
                     /locus_tag="ACG8MY_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0430245.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9426..9785
                     /gene="rplT"
                     /locus_tag="ACG8MY_001028"
     CDS             9426..9785
                     /gene="rplT"
                     /locus_tag="ACG8MY_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0430246.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10037..10502
                     /locus_tag="ACG8MY_001029"
                     /pseudo
     CDS             10037..10502
                     /locus_tag="ACG8MY_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(10669..11856)
                     /locus_tag="ACG8MY_001030"
     CDS             complement(10669..11856)
                     /locus_tag="ACG8MY_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0430247.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            11962..12849
                     /locus_tag="ACG8MY_001031"
                     /pseudo
     CDS             11962..12849
                     /locus_tag="ACG8MY_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            12897..15026
                     /locus_tag="ACG8MY_001032"
     CDS             12897..15026
                     /locus_tag="ACG8MY_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0430248.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            15001..15690
                     /locus_tag="ACG8MY_001033"
     CDS             15001..15690
                     /locus_tag="ACG8MY_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430249.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(16098..16784)
                     /locus_tag="ACG8MY_001034"
     CDS             complement(16098..16784)
                     /locus_tag="ACG8MY_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0430250.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(16920..17114)
                     /locus_tag="ACG8MY_001035"
     CDS             complement(16920..17114)
                     /locus_tag="ACG8MY_001035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430251.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(17164..17604)
                     /locus_tag="ACG8MY_001036"
     CDS             complement(17164..17604)
                     /locus_tag="ACG8MY_001036"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0430252.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(17608..18453)
                     /locus_tag="ACG8MY_001037"
     CDS             complement(17608..18453)
                     /locus_tag="ACG8MY_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0430253.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            18602..20020
                     /gene="pepV"
                     /locus_tag="ACG8MY_001038"
     CDS             18602..20020
                     /gene="pepV"
                     /locus_tag="ACG8MY_001038"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0430254.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         6071 a         3842 c         4191 g         5929 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
     1501 tttgtgaatt tcagcttatt tccgaaggag ttgcttttgc ttccgccgtt tatacgtgtt
     1561 tagagcgtgg gataaaagtg ttctttactt ttgtcccacg ctcttttctg tgcgtcttcc
     1621 atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc cggaaaagat
     1681 cagtgattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat aaagcagacg
     1741 aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt gattcatcag
     1801 atgcgtcagt acggttcctg aagtacccac tgcatttgcg ccgagacaaa gggctttaaa
     1861 tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag tgtctgcttc
     1921 atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat aactcagttc
     1981 ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc gaccgctgat
     2041 atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg tcatgccaaa
     2101 accgacttct tttacgatga gggggacgtc aatggctgtt tgagtctctt gaataagtac
     2161 tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag gtgcattcaa
     2221 atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tcttgggctc tttcgacaga
     2281 agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc gcataatcgt
     2341 ataagtatcg gctaaagaag ggtctttcaa ggcagcgctg actgaaccag tggcaatcat
     2401 caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg ttttttcgct
     2461 tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa ctgcctgtgt
     2521 acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga tccgtacgaa
     2581 gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat gttcgtcttt
     2641 tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag tggagtgatt
     2701 tctgcttttt cccatgcact cattaaagga aggatgccag atttctggtc aacaattacg
     2761 attccgcaat cgccaccacc tgcaccagaa gattttgcgg ctccttcata ctgttcagct
     2821 aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat tgcagataaa
     2881 tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac attattttct
     2941 ttaaaccctt tgatcatttc gttgacacat tctgtactgt tttttaagaa aagctgataa
     3001 gccaccattt tgtcttctct cgaacggtga acttgatcga ccaaatcaga agtagaggca
     3061 gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa tggctcaatg
     3121 gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt ttcttgttct
     3181 tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca gctagcagcg
     3241 atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc aatcttgaaa
     3301 atctccaatt gagataaatt caacgcgtaa aaaacattca aggctttgac cgttgcaacg
     3361 gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc taactcactt
     3421 gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata cttttcagtc
     3481 aaacgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa aactaattct
     3541 ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg gatacttccg
     3601 acttttcgtg cggattctac agtgactgtt acgaattgat cgactgcagc gataactgct
     3661 ggatgtcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc tggtgcagat
     3721 acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc agcatgggca
     3781 gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc agataaatgg
     3841 ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta gcatggaatg
     3901 cctttttttc tcaattgccg caccgcatcc atcgctttta agctgtcagg agaccagtaa
     3961 gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt tctttccatc
     4021 gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg atatagatcc
     4081 ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt tcgacgcatc
     4141 ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac cattgctaaa
     4201 tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc gtcatggcct
     4261 ttttcccatt caacgaaacc accgaaaata cttcggcaag ctgaaccaga cccgcgtcga
     4321 gcaaatcttg aaagagagag atcgtctaat ccaagcttaa gagcagtatt gcaagctcct
     4381 gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg aacgaaattc
     4441 tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag gtcaagaaac
     4501 tgactgactt tttttgttgc tttttcgtct tgtaaggtgc catctagata aaattcatct
     4561 accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc caatgttaac
     4621 gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca atatttgatt
     4681 agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta ctcctaatcc
     4741 ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt cttgggcttt
     4801 tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag ttaatttggc
     4861 acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat cattgctgat
     4921 tgtcagcttt tccagaagtg actgtgattc atccattgtc tgtgccaacg tttctggtga
     4981 attctcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt ggatcatttg
     5041 tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg cttctcttgt
     5101 ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca aagaaaagta
     5161 gtcaaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag cggctgcatc
     5221 gattccacta ggattaccgt gggcgatttt ttccgaaagc tggacatttt ctaatagtat
     5281 ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg ctgtggcgac
     5341 tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg tcagttgcaa
     5401 gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga tattttttaa
     5461 tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt gatcgtccat
     5521 tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa aggcaatcgc
     5581 tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc ttgattctcc
     5641 ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt taagcaaaaa
     5701 ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact aagggtctca
     5761 aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa gaatacgttt
     5821 tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc tttgactaga
     5881 aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaaggtt ggagttaaac
     5941 acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt agcaggtaca
     6001 attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga tagtcaggca
     6061 ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa ccctgaacag
     6121 tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat caaacaagca
     6181 atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact gcttttccat
     6241 ctaaatgcag atctaggaag agcaacagaa acagaagttt gtgtgtatga tgaactgctg
     6301 gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga tttattagtt
     6361 catattcgtg tatcttttga tacgatgtta gagcgtatcg aaaaacgtgg tcgttcgtat
     6421 gaacaattgt cctttgaccc aagtctatac gattattaca aagaattgaa cagacgttat
     6481 gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat
     6541 aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga agaaaaatta
     6601 gcagagattc gccaaaccaa agtatccatt tgatttacag ttgctcaaat aaagggtaaa
     6661 cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg atagagtatg
     6721 cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat
     6781 gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat gctgacttat
     6841 tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga agcgttactg
     6901 cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac tagtcctatc
     6961 gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac cgagtacgtg
     7021 attcgactaa ttcgatcgct tttggaacgg atgccagaca tgagtgtata cagtttattg
     7081 tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga agtgaatagt
     7141 gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa
     7201 ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca aaatgtagta
     7261 cttttagaac aaatcaaggc actggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa
     7321 gagttggacg atgcactgat cgaagcacga caagcagttg aaatgacgaa cttagcttcg
     7381 cagattttag accagctctc tggcacttat aataatttgc tgaataacaa cttgaatgac
     7441 acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat tgtgacagga
     7501 ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg ggaaatcgcc
     7561 ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg gcggattcgt
     7621 tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct tgctcttgat
     7681 tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgttgtgaag aaattagtcg
     7741 taaagtctaa tcccttcaga gaagttcgaa accagttcga accggttaac tgattgtcag
     7801 acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat tgacaatgat
     7861 cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca atacaatcag
     7921 tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat ttacattgat
     7981 gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa tcacattttt
     8041 caaaaatgac attactgcat tcaaaatgac gtaaacctgt tttttgcatc gtaagttttt
     8101 ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg ttacctccgt
     8161 tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc tttgtaagaa
     8221 gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc gtttttcttt
     8281 agtatagcaa agaagcggga atcactgcta gaaagcctgc taatctaatc attttcagaa
     8341 aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct atactataat
     8401 ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct
     8461 agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt
     8521 tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga
     8581 ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg
     8641 gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac
     8701 aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca
     8761 tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac
     8821 aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca
     8881 atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta
     8941 tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct
     9001 tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca
     9061 gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct
     9121 gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa
     9181 acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa
     9241 acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca
     9301 attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc
     9361 aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat
     9421 tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa
     9481 aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag
     9541 taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta
     9601 aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat
     9661 taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg
     9721 ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta
     9781 aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt
     9841 tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaataac
     9901 ataattaagg ttttttaatt gaaaactgaa catttatggt catcctttaa tgaaagcgct
     9961 aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa
    10021 tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt
    10081 tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttt gtcatcatgg
    10141 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac tcggcgatgg
    10201 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
    10261 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
    10321 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
    10381 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
    10441 gaccagtgat ttctattgca gttattgtgt tgccgatctt gatgtcatta ggtatttcag
    10501 tcaagtccac aatcttgtgt aaaagttcaa cctcatcaaa agcacaatgt tttgcgttgt
    10561 gaacctaact gccatgcctt tctttctgag gagagaggtc aggagcagta gcgcagcgac
    10621 tcttgatgtc tctcctcaga aagaagagac tcgcacaggt tcatggtgct aaatgttatt
    10681 cgaattaaat tctaaatatt ttcgtggaga agccagccac tcagtgtctt tttccatcaa
    10741 aagtgttcca attatccgag tgatggaatt gatatttgga aaaatccgaa tcactcgttc
    10801 tctccggcga atctcttcgt tcaaccgttc caacacattg gttgtacgaa tccttcggcg
    10861 gatattttca ggaaaagaca aaatttgaat agcatcttca aagccgtttt ctaaaatgtc
    10921 gcaagcagca cttaattttg aatcacaacc atatttttcc aagaagtgct cttttctttc
    10981 acgtgtcaat tcaagttcag aggctttaaa gatactcttc aactcgtctt ttacatcaga
    11041 agaaactttc tttggcagct tatcaaaaat atttctcaaa aagtgggctt ggcatctttg
    11101 ccagctcgca cctaaaaagt tcttgcgaac agccttaact aaccctttgt gtgcatcaga
    11161 aatgaccagt tttacaccga acaaacctcg ttgaacgaac gattgaaaaa cggtatccca
    11221 attatcttcc gattcgccgt cttgaacatc aaaaccaaga atcgtacgtc gtccatcaga
    11281 atcaatgccg ataccaatat atacaccttt tgagacaata cgatgatttt cccttacttt
    11341 catatacagg gcatcacaca tgaaaaatgg atattccgag ccttctaggc tccggtttct
    11401 ccactcaaaa acaaatggat ccaactgttt catcagattt gaaacgaatg atttagaaac
    11461 agtcgcacca tccgttagca gttcaactgt tttagttact ttgcgagtgg agacaccaga
    11521 aatgaccatt tcaatcatgg cagcgatcaa agctttttcg cttcgctgat atctttcaaa
    11581 gatagtagga ctaaacttac cgtctctggt tcgtggaaca tgaagttcta acgtaccaat
    11641 tcgagttgta aacgaacgat cgtagtaacc atttctttgt gattgccgtt cttctgaacg
    11701 ttcataggca tttgcctgaa tatattgatt tcgttgttct tccatcagct gattaaacat
    11761 gatagtcata agtgtttttg cggtatcatt cgcaccgctt tcgtttataa ttgcttgaat
    11821 ttcttcgctt tcaaaagtaa aatgtacttg agtcattgat atcagtccta ttcgagtatg
    11881 ttttctgcaa aaaacattgt actcctaact gagatcaatg gcttttttga tgcttcactt
    11941 ttacacaatt atatggacgt aatcggtatt tcagtaccgg ttgcactgtt ctcatttatg
    12001 ggctcgatta tggctggaat ctttgctaat atcgtcaatt tcaaacaata tcaaacgatt
    12061 tatgctggtt tcaatccagc ggctgaaagc tatacttata atgactattt ccaaattggt
    12121 atgattggga tgattgtaag cttagttgtg gtactaacgg tcgctaatat atcaatgaac
    12181 aaaaagaaac gctatgcaat ggcagcaaac gtaccagctg aaggtggcga cgcaccaatg
    12241 atctcatggt tagccgtatt attaccagtt ttaggggtcg ttcttttaga tcttcaaatc
    12301 atcttaggtt ttattctagc aggtatctgg gcattgctgt ttactggaaa attgcgcgga
    12361 ggttataaag aaatctgtcg tcagtttgcg aaattattta cagatggcgc agtcgatgtt
    12421 gcaccaatgg ttggtttctt gatgacctta gcgatgttca ataattcagc tgcgtatgct
    12481 tctccttatt tttcagctat ttttggagat tggataccgc aatcaccatt agtcttggca
    12541 atcgtatttg ctatattgac accactagga tttttccgcg gtccaatgaa tctagtcggt
    12601 tcaggctcag ctattttagc tgtcgtgtta gctgttaatc caacgatgtc gccagccttt
    12661 ttgttcccgt tatttgcaat tacaacgatt gctcctcagc atttagatat tacacaatct
    12721 tgggttgctt ggggattagg ttatacaaaa gtaacttctc gagaatatat gaagaaatcc
    12781 atcccaactg gttggatcat cggtgcgatc ttatgcctga tcacgttctt attgtacggg
    12841 aacgcctagt aaacaaaacg tcgataaaaa cgattagata aatggaggaa aagaaaatga
    12901 aacgttttgt gcgaatggga attgatgttg gcggtacaca tacgaaagca gtcgcaatcg
    12961 ataatgcgac gcacgaaatc atcggaaaat cttcagtgaa aacaacccac gatgatgtcc
    13021 gtggagtagc tgcaggtgtc gttcagtctt ttcaaaattg tttgcgggaa aataatatca
    13081 gtccagaaga tgttgtgttt gttgcacatt ctaccacaca agcgactaat gcactgattg
    13141 aaggagacgt agcaaaagtc ggcgtaatcg ggatggcaaa aggtggatta gaaggttttt
    13201 tagcaaaacg acaaactaga ttgaacgata tcgatttagg gaataagaaa aaaatagaga
    13261 ttgtcaatgc attcttacct gtcaaaaact taaatgtaga ccgtgtttca gaaacgattt
    13321 cttctttaga aagagaaaga gcggaggtct tggtttcttc tatggcattt ggagtagata
    13381 atggggaacc agaacgagtt gtttatgaag cagcatctgt gaaagcgatc cctacaacga
    13441 tggcttctga catcacaaaa ttatatggat tgacaagaag aactcggaca gcagcaatca
    13501 acgcatctat tttaccaaaa atgctagata cggcaacctc gacagaagac tccgtacgag
    13561 aagcaggggt caatgtttct ttgatgatta tgcgcggaga cggcggagta atggaaatca
    13621 atgaaatgaa aaaacgacct gttttaacca tgctttccgg tcctgctgca tcagtcatgg
    13681 gctctttgat gtacttgaga gcgtctaatg gtgtttattt tgaagtaggc ggaacaacaa
    13741 ccaatatcgg ggtcattaaa aatggacgtc cagcaatcga ttattcaatt gtaggtggcc
    13801 acccaacata tatctcttca ttagatgttc gggttctagg tgtggcaggc ggttcgatgg
    13861 tacgggctaa tcagtcagga atcatcgatg tcggtccacg ttctgcacat attgccggtc
    13921 tagattatgc agtatttacg gagacagaaa aaatcaaagg tcctaaggtt gaatttttct
    13981 ctcctaaaga aggagaccct gctgattacg tcaaggtagt gatggaagac ggagaagaag
    14041 tgactattac aaatacatgt gcagccaatg ttcttggatt agtgcaagaa gaacattttt
    14101 cttatggaaa tgtcccatct gcacgaaaag ccatacaggc attagcagac tattgtcata
    14161 cgacggttga agacatcgca gagcagatca tggaaaaatc ctatgctaag attgaaccgg
    14221 tgattttaga attggcagac aagtatcact tggagaaaga tcagatttct cttgtaggtg
    14281 tagggggcgg cgctgcctca ttgattactt actttagtaa taaaatgggt gtgaaatatt
    14341 cgattcctga aaacgccgaa gtgatttctt ctattggtgt agcacttgca atggtccgag
    14401 atgttgtaga acgtatcatc ccttcgccaa gtaaagaaga tatacgatcg ttgaaaaatg
    14461 aagcgatgaa taaagcgatt gaatcaggcg ctacaccaga atcgatcgaa gtccacgtag
    14521 aaatcgatcc acatacttca aaagtaacgg ctatcgctac tggttctacg gaagtgaaag
    14581 caaccgatct aaccaaggag atcacaacag aagaagcact agagcttgct gcagaggata
    14641 tgcgattaaa taaaaatgaa gtttgcttat tggaaaatac acctttcttt tatgtatgcg
    14701 gcgaacagaa tcgttcaaaa aatgctggaa gtcttcggat catcgatcaa aaaggattta
    14761 tcaaggtcca acgtggacat gcttcatgta tgaaaacaac tgcagccaat tatatgacag
    14821 ctgtcgaaca actttgggaa gatatggcag tttatcaaac tgaattgatt gcccgaccag
    14881 aattttatct ctgcttagga gcaagagtct ctgattttac agcgacggat ctggaacaac
    14941 tacagctttt gatggatctg gaagtatcta ctatggaacc ggaggaagaa gtgatcgtcg
    15001 ttgccggaaa tatcaaacaa acataagtta gaagagttac taaacgaatt acaacagatc
    15061 cccgaagaaa tttggggatt ctaccagttt cggcgagatt tattttggaa aaagattcct
    15121 ttgtccaagc agaaaattct tatacaacaa tcaatcgatt gtggtatcga aacggcatgt
    15181 agcatcaaaa aaaagtaccc ttttgccgat gtaggagaaa tctgtgaaca aatggctgtt
    15241 ccaatcgttt cctgtgaatc agagcaaata aacgaacgaa tcacttttgc aacttatgca
    15301 gaagatgaag ggatccgatt gatgacggag ccgttagaaa aattaaaatg ttcaggacta
    15361 gcttcgatct ctaaagaaac agcgcaagct ttgattattg gacatgaact atttcaccac
    15421 atcgaagctt ctgttaaggg aatttatacg caaagcgaaa aaattgtgct gtggcggttg
    15481 cctttttata cacatcaatc aaacattcgt gcattgagcg agatagcagc gatgagtttt
    15541 tcaaaagaga tgaatcaaag cgatttttcg ccttatgtat tagaggcagt cttactttgg
    15601 ccatataatg aaattcgtag ccaagggatc ttagaagaaa taaaagaaat agaaaaaaga
    15661 tgtacggagt atgatttaga ttacaaataa agagactgtg acaaaagtcg tgtcacagtc
    15721 ctttttttcc gaataaacgg tgttctcctg tcaactcctc ggtcacaagt caaaatattc
    15781 aacaaacatg agaagctgtt ttttgaaatt gttccttgtg actcggagat gaacggcagg
    15841 ctcacgacct cttggaaaaa ggtgttcaaa agcttgcatc tgcggtaata agaacagcca
    15901 agttaaaaac ataagaagct atttttactt ggctgttctt attacttgat gcagggcagc
    15961 tttttcacaa cctctcaata aacggtgtgc aatcagctga ccctcggtat tagctcaaaa
    16021 aaaacggcaa aatatgagga actattttgc cgtttatttc tctaatactc aggtcaaaac
    16081 gctgattcca aaacctctta tttgatgggt ctgtcctgaa tatcttcaaa ataatcgggg
    16141 taattatgaa tcacgtattc ttgctcctcg ataatcatat gtaaatgcat cgtgcaaaga
    16201 aatccgactt catcaaagtt atgatcaatc attgattgaa gcattgtctg atgttcatct
    16261 aatactcgac cccagtctaa ttctgaaatc gtcaaacgta accatctgaa acggttcaag
    16321 tgcgtactat aactttctag ccaatcccaa atttctcgtt ttccagcaat ttcataacaa
    16381 gttcggtgaa aatcagtatc caaatcaaaa aaagcttttt tattttgagc gatcactgcc
    16441 tgatcggttt gttctagtat tttttctagt ttagcttgat cttgcttggt cattttcgca
    16501 ctgcattctt gcaggatcgg tttttccaac ttttcccgcg tataacgggc taatgaagca
    16561 gatcggatat cgatttttga aacataagtg ccggactgtg gaatggtttt tacaagctct
    16621 tgattgcgca gttggatcag tgcttctcga atcggtgtac gcccgatagc tagttgttct
    16681 tctaacactt tatcggagat tttacttccg ggtgctaact cggaataaat gatttgttta
    16741 cgtatagatt cataagcttg ctgttgcaaa ttattgataa ccatttaacc ttcctttcta
    16801 tgcagatata atctattata acataaatcg cttacaaatc gtgtgggcat aaaaacacag
    16861 tctgcaaaaa aagccctgat aggcatgcct attagagcct cgaaaagaaa aatattgaat
    16921 caatctggtc gtacctcttg gatcggtttg acacgaccgc cgattctttt agcaaaagct
    16981 tcggattctt ttttgtcttt gaaaatgagc agattgtcta agtttttttc ccgagtgcga
    17041 cagccagttt tatcgatgta accgtgcatt actttaacga catacatggg gatcacctgc
    17101 ttttttcttt tcattatacg aactactcga cacaatacaa gaaaaatgct tagacggtct
    17161 ttattaagaa tttacatcat ccaaagaagc gtacgcttgg aataactgtt ctaactccgg
    17221 attgactttg ctcagcttca ctaatcgttg cgaatcagaa agtaaaaaac agtgtttact
    17281 actgccagta gtttttaatt ttttgtcttc agttcccaca atttcgtagc taaaatccaa
    17341 ccgtgttccc gtatactttt ggatagttgg gtggatgctc actttttctc cgtaatggat
    17401 catttcttta tagctgcaat ttacttccag aacggggatg atgatcccag attcttcgat
    17461 ttttttatag ggaaacccta gatgtaccaa taaggctacc cgagcctctt caaaccagcg
    17521 gatataattg ctgtgatgaa taatccccat ttgatctgtt tcataataaa aaggttctcg
    17581 cacataacct gtaaatttct ccatgactca tgcctcgttt tccattgatt ttttacaaaa
    17641 ataattcatc cacttcggaa gtgcttcgct gatttgtgtc ggcaaaacaa tatattgttc
    17701 ttttgccagc tggtctccaa tgaaactatg aagataaaca gctgctgcaa tcgtttggtc
    17761 attttgagga aactgggcta aaaatcctgt gatcatccca gctaaagtgt cacccatgcc
    17821 gcctgttgcc atagcagcag aacctaaagg attggtgtag aagtgctggt gaccataaat
    17881 ttttgtctca tgacttttca aaacgagcac agcaccgatt tcctgttgct tctttttgtt
    17941 ttctttatcc cgttgttctt gtatcggtag agaactcaac cgttgccact ccatttgatg
    18001 aggtgtaagt acagtttgtt cgggataggg tagcaaaaga tgattagcaa caagtaaact
    18061 taaagcagag ccatcaatca ccagccattg ttcttttgat tggttttcta gtacttgttt
    18121 gaaacgttcc aaactcttag aagaagtacc caatccggga ccaatcaaga gtacatctgc
    18181 ttgctttatt acggaaagaa actggtcagt atccgtccaa tctacaatca ttgcttctgg
    18241 cagatgactg tgaagagcac tccgattttt ttcatcagtt actacagtcg tgagacccgc
    18301 tccactgtgt acggcagcta aagcactcat aatgattgct ccgccatatt gttcattgcc
    18361 gccaatcaaa acgatacgtc caaatgttcc tttatgtgaa atctttggac gaggtgtgat
    18421 gactcttttc actagttgtt cgtttatcag catcgaaaaa cctcctttta ctcattatag
    18481 cgtatatttt ttttagagaa aaacagataa gaaaaggaat tgtcagattt tttgaactat
    18541 ttttttctgt aatttgtggt atcatgaaca cgaagactta aataagtgga ggattaactt
    18601 tatgacaatt gattggcaaa aagaagtaga agcacgtaaa gaagacttgc ttgaagactt
    18661 gaaaaatctt ttacgtgtca acagcgagcg cgatgattca aaagtaacac cagatgcacc
    18721 atttggacct ggacctcgcg acgcattgaa acatatgtta gcttatggcg aacgtgatgg
    18781 atttaaagta aaaaatgtcg ataactatgc agggcatatt gatcttggcg aaggagacga
    18841 aacgctaggg atatttggac acatggatgt cgttcctgca ggagatggct gggacacaga
    18901 tccttatgaa ccagtaatca aggatggaaa gatctttgca cgcggatcaa gcgatgacaa
    18961 aggtccaagt atggcagctt actatgcgat gaagatcatt aaagaacttg acttgaaact
    19021 atcaaaaaaa gttcgcttcg tagtcggcag cgatgaagaa agcggctggg gcgatatggc
    19081 ttattatttc gaacatgaag aagaaccaga ttttggtttc tcaccagatg ctgaattccc
    19141 aatcatcaat ggtgaaaaag gaaatgtttc tttagcttta cgcttcaaag gcgacaatgc
    19201 aggagattat gtcttgaaat cttttgtttc cggacttcgc gaaaatatgg tgccaggaac
    19261 agcaactgca gcacttcaag taccaagtgc agatgcagct atcgcgatgg aagaagcatt
    19321 ttaccaattc atcgaagcaa atccagtaag cggaacgatt gaagcggaca atacgtatgt
    19381 aaaaatcgaa ttagttggta aaggcgcaca tggtgcaagc ccacaatctg gtatcaatgc
    19441 aggttctttc ttggcattgt tcttagataa ctatgaattc ctaggatctg ctaaacaatt
    19501 tatccatgta gcagctgctt atgtccatga agatttttat ggagaaaaac taggagtcgc
    19561 ttatgaagat gaaaaaatgg gcaaattgac aatgaatgct ggactatttg cttttgaaga
    19621 aaatggtaca gaggaagcga acttcatcaa tatgaatttc cgttttccaa aaggtgtaac
    19681 agtagacgga ttgcaaagtg acatcgaaca aacagtgggg caagaaggcg caacagttac
    19741 tcgaggtgct cgcgtaatgg aaccacatta cgtaccaatg gatgatccat tggtggctac
    19801 attattgcaa gtctatgaag accacactgg agaaaaaggc tacgaacaaa tcatcggtgg
    19861 cggaacttac ggacgtttgt tgaaacgtgg cgttgcttat ggagcgatgt tcccaggata
    19921 tactgataca atgcatcaag caaacgaatt tatgagtctt gatgacttgt tcagagcaac
    19981 agcaatctat gcagatgcca tctatcgttt ggcgaaataa aaatactaaa aaa
//
LOCUS       DAZKNO010000039        20023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000039 DAZKNO010000000
VERSION     DAZKNO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.039"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..494
                     /gene="mraZ"
                     /locus_tag="ACG8MY_001039"
     CDS             63..494
                     /gene="mraZ"
                     /locus_tag="ACG8MY_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0430255.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            515..1474
                     /gene="rsmH"
                     /locus_tag="ACG8MY_001040"
     CDS             515..1474
                     /gene="rsmH"
                     /locus_tag="ACG8MY_001040"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0430256.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1479..1880
                     /gene="ftsL"
                     /locus_tag="ACG8MY_001041"
     CDS             1479..1880
                     /gene="ftsL"
                     /locus_tag="ACG8MY_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0430257.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            1881..4073
                     /locus_tag="ACG8MY_001042"
     CDS             1881..4073
                     /locus_tag="ACG8MY_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0430258.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSTYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4099..5061
                     /gene="mraY"
                     /locus_tag="ACG8MY_001043"
     CDS             4099..5061
                     /gene="mraY"
                     /locus_tag="ACG8MY_001043"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0430259.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5075..6436
                     /gene="murD"
                     /locus_tag="ACG8MY_001044"
     CDS             5075..6436
                     /gene="murD"
                     /locus_tag="ACG8MY_001044"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0430260.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6462..7550
                     /gene="murG"
                     /locus_tag="ACG8MY_001045"
     CDS             6462..7550
                     /gene="murG"
                     /locus_tag="ACG8MY_001045"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0430261.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7565..8785
                     /locus_tag="ACG8MY_001046"
     CDS             7565..8785
                     /locus_tag="ACG8MY_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0430262.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            8941..10266
                     /gene="ftsA"
                     /locus_tag="ACG8MY_001047"
     CDS             8941..10266
                     /gene="ftsA"
                     /locus_tag="ACG8MY_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0430263.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10289..11530
                     /gene="ftsZ"
                     /locus_tag="ACG8MY_001048"
     CDS             10289..11530
                     /gene="ftsZ"
                     /locus_tag="ACG8MY_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0430264.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11547..12224
                     /locus_tag="ACG8MY_001049"
     CDS             11547..12224
                     /locus_tag="ACG8MY_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0430265.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12238..12837
                     /locus_tag="ACG8MY_001050"
     CDS             12238..12837
                     /locus_tag="ACG8MY_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0430266.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            12918..13139
                     /locus_tag="ACG8MY_001051"
     CDS             12918..13139
                     /locus_tag="ACG8MY_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0430267.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13188..13970
                     /locus_tag="ACG8MY_001052"
     CDS             13188..13970
                     /locus_tag="ACG8MY_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0430268.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            13991..14698
                     /locus_tag="ACG8MY_001053"
     CDS             13991..14698
                     /locus_tag="ACG8MY_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0430269.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            14960..17758
                     /gene="ileS"
                     /locus_tag="ACG8MY_001054"
     CDS             14960..17758
                     /gene="ileS"
                     /locus_tag="ACG8MY_001054"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0430270.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(17807..19333)
                     /gene="zwf"
                     /locus_tag="ACG8MY_001055"
     CDS             complement(17807..19333)
                     /gene="zwf"
                     /locus_tag="ACG8MY_001055"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0430271.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19348..19995)
                     /locus_tag="ACG8MY_001056"
     CDS             complement(19348..19995)
                     /locus_tag="ACG8MY_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0430272.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
BASE COUNT         6786 a         3484 c         4308 g         5445 t
ORIGIN      
        1 tggtagaaag tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt
       61 caatgttgat gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct
      121 caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc
      181 ttttcggcta tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt
      241 tggctaaaaa agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg
      301 aacttgataa acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga
      361 caaaaggttg cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat
      421 ggcaagattt ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg
      481 atttcggact ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac
      541 agtcatgttg aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga
      601 ctgtacactc ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg
      661 gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc
      721 ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga
      781 acgcttgagt gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc
      841 tcctcagcta gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat
      901 gcgcatggat cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca
      961 tgaattagta aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg
     1021 tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat
     1081 cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat
     1141 ttttcaagct gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga
     1201 acaggcaatc cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt
     1261 ggaagacaga attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc
     1321 gggacttcct gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc
     1381 aatccttcct tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg
     1441 tattgcagaa aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc
     1501 aagattacga atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt
     1561 cttcttcgaa tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt
     1621 ctgctttgga aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt
     1681 taacagttat gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa
     1741 cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt
     1801 ctcgtactga acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca
     1861 atttaaggaa agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga
     1921 aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta
     1981 ttggattgtt ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg
     2041 ctggtacctc attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag
     2101 caaaaagagg aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt
     2161 attcaattaa agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg
     2221 aagaaaagaa ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa
     2281 aagatgcact gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg
     2341 gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa
     2401 aagaaggtgt cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg
     2461 tcttttcttc tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg
     2521 gtaaactagg tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta
     2581 tttatcaaaa agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag
     2641 ctgtcgacgg acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa
     2701 cactgatgga tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga
     2761 aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa
     2821 tggaaggatt gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg
     2881 aaccaggatc aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca
     2941 acgagaatga aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc
     3001 atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg
     3061 tcgggatggt catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac
     3121 tgggatttgg acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta
     3181 cattaaatat tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt
     3241 tccaaatgat gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat
     3301 atatcagcaa agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc
     3361 tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg
     3421 tcgaaagcga aaactacggt agtacttatg gtgtatatag tgtacctgga tacaatgtat
     3481 cggcaaaaac cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata
     3541 cagcctacct gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt
     3601 atctaacaat gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc
     3661 cattgatgaa acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa
     3721 aaacagaaaa agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg
     3781 atgctcaaaa aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac
     3841 aatctactgc caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg
     3901 gtgataagct gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga
     3961 aaatcctcgg tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg
     4021 ctccatatga aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga
     4081 aataggagag aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt
     4141 gcaatgacaa ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt
     4201 caggcgattc gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg
     4261 ggcggcttag tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa
     4321 aaacagctaa ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc
     4381 ggctttcttg atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag
     4441 cagaaattga ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa
     4501 ggttatccag gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt
     4561 attttcgctc tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc
     4621 gatggattag ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg
     4681 catcagcatc agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt
     4741 ttcttcgtct acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg
     4801 ttgggcggac ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg
     4861 attggtttga tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag
     4921 cttacaggaa aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga
     4981 tggtcggagt ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc
     5041 acattatgga tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa
     5101 taagaaagtg cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca
     5161 tgaattgggc gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc
     5221 acaagagctg ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt
     5281 ggatgaagaa ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt
     5341 agcaaaagcc caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt
     5401 agcggaatgc ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat
     5461 gacaggacta ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat
     5521 tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga
     5581 attgtctagc ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac
     5641 caatatctat gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa
     5701 atggaacctt caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc
     5761 agaactacaa gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga
     5821 agtgttggaa gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat
     5881 aatggaaata tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc
     5941 tatttctgta gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt
     6001 tttccatggc gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta
     6061 taatgattca aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac
     6121 gtctaaggtc gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat
     6181 tccttcatta aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat
     6241 ggatgctgga aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc
     6301 agttccttta gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa
     6361 tgccagctgg gatcaatacc caaattttga aactagagga aacagattca tggaagctgt
     6421 gaaccgatta aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag
     6481 gcggaggaac agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc
     6541 aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg
     6601 tccctgatac aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt
     6661 ccatgcataa catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa
     6721 tcctcagtga ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg
     6781 tagtgtacgc tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac
     6841 caggtgttac caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag
     6901 atgcggcttc cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag
     6961 aagtagcaga tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga
     7021 aaacagtctt gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag
     7081 tttttttaag tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat
     7141 attatgaaga gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt
     7201 atatcaaaaa tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg
     7261 caacttcgat cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat
     7321 atgttacgaa tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa
     7381 aaatgattgc agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca
     7441 tgggtgatga agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg
     7501 atgcttcaaa acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt
     7561 cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa
     7621 tttgactcct tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca
     7681 agagccaaaa aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa
     7741 aacggttcaa gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga
     7801 agagatcaag caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc
     7861 caatttaaaa agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt
     7921 attgggtata ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc
     7981 agttgcagta tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt
     8041 gtcattagga gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct
     8101 taaaaaagca cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt
     8161 cgatttggat gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta
     8221 cccagtgatt gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct
     8281 ccctatcttg gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa
     8341 tcagttatca tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag
     8401 caataaaaat ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa
     8461 taatcttgcg aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg
     8521 tgtgatcgac atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga
     8581 agaatcgggc caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga
     8641 atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg
     8701 atcatctgat tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac
     8761 tccgtcaagt ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta
     8821 aaaaccagta atttatggta gaattggaag cagtgaatat atctattaat aagtatattt
     8881 taaaacggac gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc
     8941 atggcaaaaa caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc
     9001 gtggctgaat atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa
     9061 gggattaacc gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca
     9121 gtacgacaag cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca
     9181 gcaaacatgc ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag
     9241 gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct
     9301 cctgaacgtc aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga
     9361 atcaaagatc ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca
     9421 ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc
     9481 aacgaaatgg tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa
     9541 gattttggta cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat
     9601 gataagcaat tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat
     9661 atctctattg tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg
     9721 gatgcatacc cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag
     9781 tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa
     9841 cagattttca acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt
     9901 ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa
     9961 atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta
    10021 tttacaaatg taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg
    10081 gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa
    10141 acaacaaaca catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa
    10201 ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt
    10261 gattaattca tcctgggagg aataagatat ggaattctca attgataata atatcaatga
    10321 cggcgcagta atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat
    10381 gattgaagaa aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt
    10441 aaaaaattca aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg
    10501 tgctggttct cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg
    10561 cgaagcttta gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg
    10621 tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg
    10681 tgtcgtgaca cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg
    10741 tatcgctcgt ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt
    10801 agaagttgta gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt
    10861 acgacaaggt gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga
    10921 ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt
    10981 ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct
    11041 tgaaacatct attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat
    11101 gacattgttc gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt
    11161 aaatatcatt ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt
    11221 catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca
    11281 agcacaaatg caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac
    11341 ttcttcagaa gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg
    11401 tcctagagtt gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa
    11461 ccgcgaagaa acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg
    11521 taaaaaataa aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag
    11581 aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg
    11641 taacaaaatc tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt
    11701 gtgcagaaaa ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg
    11761 aaataaagtg gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg
    11821 aaattgatta ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt
    11881 tagagaaaga attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg
    11941 gaatttcacc agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca
    12001 aggttgttgg gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag
    12061 tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac
    12121 catgtagtga attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg
    12181 caacatttat ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg
    12241 ccgcttttaa acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac
    12301 gaataccaag attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca
    12361 acacagaatc aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg
    12421 gcgacttcac gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt
    12481 gaactacatc aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct
    12541 acacaaccta aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct
    12601 gaagctatgg cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat
    12661 ttagtagaag aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca
    12721 ctagatggag atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg
    12781 gagattgaca gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag
    12841 gaggaactac gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt
    12901 tctatagcat tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat
    12961 cagggctagg acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc
    13021 tgcctttgag gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact
    13081 tagccgtaca aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat
    13141 tgaagcaaca aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg
    13201 tataccagca ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg
    13261 aacaggttca aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct
    13321 atattttaga aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat
    13381 atgaacaggc agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg
    13441 aagattttga aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc
    13501 atgggaaaat ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata
    13561 tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg
    13621 ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag
    13681 aaatgattat gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac
    13741 tagacagtgt gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg
    13801 aagctggtaa agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac
    13861 tattagatat tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga
    13921 agacgaaaaa agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg
    13981 tggtttactc atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat
    14041 gcggggatat aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga
    14101 ggaattgact caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact
    14161 tgagtacttc aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac
    14221 ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca
    14281 aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga
    14341 tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc
    14401 gacagaaact gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat
    14461 gcttgagtct caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt
    14521 ctccagctac gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga
    14581 caatgaaaat gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc
    14641 atttgataca caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa
    14701 cagaaaagca ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac
    14761 tgcatggtga aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc
    14821 ggctgacctc gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt
    14881 ggtaccacgg catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa
    14941 taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt
    15001 ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa
    15061 tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga
    15121 tggacctcca tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa
    15181 agacattatc attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg
    15241 ggatacacat ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga
    15301 aatgtccatg gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa
    15361 gcaacgagca gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac
    15421 attagatcct tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaagg
    15481 ctatatttac aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc
    15541 ggaagctgaa attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt
    15601 caaagacggc aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc
    15661 atggacattg ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt
    15721 ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga
    15781 aatcggttgg gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat
    15841 gactgcaagt catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac
    15901 tttagacgct ggtactggat tagtccatac tgctccagga catggggaag atgactatat
    15961 cgctggtagc aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac
    16021 agaagaagca cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga
    16081 tctattaaaa gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca
    16141 cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat
    16201 cgataaattc cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg
    16261 gggtaaaaca cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca
    16321 gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac
    16381 accagaaaca atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt
    16441 tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg
    16501 cgaatttaca aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga
    16561 agctgtttta cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc
    16621 agaccaatat cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt
    16681 agcaccatac aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat
    16741 gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga
    16801 tatccttcgc ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga
    16861 tatcttaagt caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt
    16921 agcgaataca gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc
    16981 tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg
    17041 gtacgacaaa tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga
    17101 tctatcgtca ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga
    17161 ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt
    17221 attgactcct atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga
    17281 ggagtacgtt cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt
    17341 gatggatacc tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga
    17401 agcaagaaac agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa
    17461 acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt
    17521 ttcaccagac tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt
    17581 atttgatgat gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg
    17641 ccaagttcgt aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc
    17701 aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa
    17761 gggaaaaggg ggtgtgacat gttctttgtc acaccccctt ttgttatcaa tcgtttaatt
    17821 ttccacgttc acgataccag ttatctggtt gccagatcca ttcgaatccg tctttttcaa
    17881 gtagttcgaa agctgcttta ggtcccattg atccagcttt gtagttggga aaatccggtt
    17941 gtgtttggtc ccaagctttt cgaatgacat ccacgatatg ccaagattga gcgacttctt
    18001 cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc taatagtaat ttttcgtaag
    18061 cttctgggct attttcaacc atttctgcac tatttctaaa ttccaatttg attggttccg
    18121 tttcaaatcc ttgccctact tctttcccat tcaaggataa agaaaatcct tcagttggct
    18181 gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag
    18241 atgttttgaa tacattaaca ggaacttgtt tgaatacgat gttgatccga gtcccttttt
    18301 cagtcaaacg tttgcctgta cgtacataga aaggaacacc tgaccaacgg aagttatcga
    18361 tagtgaattt gcctgcgacg aatgtttcag tctctgattg agcatccact ttgtcttctt
    18421 cgcgatagcc cttgaattgt acaccatcca attcaccttg agcatattgc ccgcgaacga
    18481 agttttctaa cacttcttct tcgttataaa tgcggatagc tttcaatgct ttgatctttt
    18541 ctgtacggat ttctttttcg gaaaatgcta ctggcggttc cattgcaagt aatgctacaa
    18601 cctgtaaaat atgattttgc accatgtctt taagtgcgcc actatgatcg tagtagcccc
    18661 cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg gatattgtcg atatagcgat
    18721 tgttccattg agcctcaaaa atattattgg caaaacggat agctgagatg ttttggatca
    18781 tttcttttcc taaataatga tcaatcctga aaatatcttg ttctgggaat acttctctga
    18841 tttgctggtt caattcaaaa gcagactggt aatcagaacc gaacggtttt tctatgatca
    18901 aacgattgaa gccatttttt gtcataatat tttgtgattt caaatgagaa acgatcgttc
    18961 caaaaaattg aggtgccata gccaaataat agacacggtt accgtttaat tgatagtgtc
    19021 catctaatcg gtcagacaat tcttttaatg tctgataatg cgttgaatca tttacatcat
    19081 gggattgata gtaaaaatgg cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg
    19141 cacggattgt ttctttgaca acttcacggt agtgctcatc gctccaggga cggcgagcgg
    19201 ttccgattac agcaaaatgc tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg
    19261 ggtacaattt acgctgggct aggtcacctg taccgccgaa tatcgtaaac aatacatttt
    19321 tttcattaga catgtggata cactccttta tattttatcg acaaaaatcg tgctggccgc
    19381 cttaaagctg acggggattt tctttctatc attttggata gtgatcggtc cttcataagc
    19441 tgcgatgtca acgatctcat attcttcgtt gatattcagg tcaatagtga ctaagtaatc
    19501 caacaattct ttttcatcaa gcactcgtgc gatacgcact tttgtaccga caggatagga
    19561 ctccaatgtt tgtcggcgtt cttcatggac aacttcctct tttttaggaa tcgatcctcc
    19621 atgaggacaa tgttcaggat gattcaaata gttttctaaa tgatctgcaa gatgttccga
    19681 agtcacatgt tccaatactt cagcatcatc atggacttca ttccagctat agtctaaatg
    19741 ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc agactgctag ctttttgcag
    19801 tccgcttgca gtcaattgta caccttgata aggggaatgt tctacaagct cttctttcac
    19861 aagtttagaa atcatttcac tgacagatgc agctgagaca tctagactag atacgatttg
    19921 tttattgttg actttcgttg tatctccacc tagttctaaa atgattttca aataatcttc
    19981 tcgatttggt gtcatattcg atcatccttt catggtaaaa aac
//
LOCUS       DAZKNO010000040        19355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000040 DAZKNO010000000
VERSION     DAZKNO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.040"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(375..1328)
                     /locus_tag="ACG8MY_001057"
     CDS             complement(375..1328)
                     /locus_tag="ACG8MY_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0430273.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1351..2106)
                     /locus_tag="ACG8MY_001058"
     CDS             complement(1351..2106)
                     /locus_tag="ACG8MY_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430274.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2103..3062)
                     /locus_tag="ACG8MY_001059"
     CDS             complement(2103..3062)
                     /locus_tag="ACG8MY_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0430275.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3055..4005)
                     /locus_tag="ACG8MY_001060"
     CDS             complement(3055..4005)
                     /locus_tag="ACG8MY_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0430276.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4155..4607
                     /locus_tag="ACG8MY_001061"
     CDS             4155..4607
                     /locus_tag="ACG8MY_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0430277.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="ACG8MY_001062"
     CDS             complement(4696..6963)
                     /gene="gshAB"
                     /locus_tag="ACG8MY_001062"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0430278.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="ACG8MY_001063"
     CDS             complement(7150..8148)
                     /gene="ftsY"
                     /locus_tag="ACG8MY_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0430279.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8159..8968)
                     /locus_tag="ACG8MY_001064"
     CDS             complement(8159..8968)
                     /locus_tag="ACG8MY_001064"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0430280.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(8977..12558)
                     /gene="smc"
                     /locus_tag="ACG8MY_001065"
     CDS             complement(8977..12558)
                     /gene="smc"
                     /locus_tag="ACG8MY_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0430281.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12578..13264)
                     /gene="rnc"
                     /locus_tag="ACG8MY_001066"
     CDS             complement(12578..13264)
                     /gene="rnc"
                     /locus_tag="ACG8MY_001066"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0430282.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13612..15393)
                     /locus_tag="ACG8MY_001067"
     CDS             complement(13612..15393)
                     /locus_tag="ACG8MY_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0430283.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15419..16333)
                     /locus_tag="ACG8MY_001068"
     CDS             complement(15419..16333)
                     /locus_tag="ACG8MY_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0430284.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16360..17322)
                     /gene="opp4B"
                     /locus_tag="ACG8MY_001069"
     CDS             complement(16360..17322)
                     /gene="opp4B"
                     /locus_tag="ACG8MY_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0430285.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17322..18266)
                     /locus_tag="ACG8MY_001070"
     CDS             complement(17322..18266)
                     /locus_tag="ACG8MY_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430286.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18267..19271)
                     /locus_tag="ACG8MY_001071"
     CDS             complement(18267..19271)
                     /locus_tag="ACG8MY_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430287.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5333 a         4191 c         3428 g         6403 t
ORIGIN      
        1 cttattgccg agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa
       61 aatatctaaa tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga
      121 ggcgatcttg atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat
      181 caacccgtat gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa
      241 accatccgta aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat
      301 aggctcttgc tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc
      361 ctctgttatt tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat
      421 tctaaaccgc ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt
      481 ttaccagcat tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct
      541 ccaccaatag cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca
      601 tattcataag aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca
      661 aagccaaata aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc
      721 ccttcattca caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc
      781 tgatcgattg cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag
      841 atctccccta aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa
      901 gaaaggaaaa tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca
      961 gaaatgatga ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt
     1021 ccacctgctg attctacttt tttataggat gaaagatact caggaaggct gcttgtagct
     1081 gctccaacta ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg
     1141 aatacgacga ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt
     1201 atcgtcaatg tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg
     1261 ttgctatttc cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat
     1321 tttttcatct cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc
     1381 aatctcacaa atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat
     1441 ttcttgggtc gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc
     1501 ataacttgca gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg
     1561 tactaaattt tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa
     1621 aggttcatcc agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg
     1681 ttggagttga ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag
     1741 gtgttccagt gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata
     1801 aggaaatcgt ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga
     1861 ttgtcgtagg atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt
     1921 atggtctaaa tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac
     1981 cgttgacttt ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt
     2041 taactgtaca tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc
     2101 aatcatagga tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca
     2161 aattcaacga cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt
     2221 cctcctgtca gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga
     2281 taagtagaaa agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga
     2341 cccacaagag ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca
     2401 aaacgatctg tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct
     2461 agcacatgtc gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc
     2521 aaaagatact gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca
     2581 tattcattcg gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct
     2641 aaaatcatcc ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag
     2701 aaaaataaca atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta
     2761 cctgtctgct gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg
     2821 acatataacg actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc
     2881 tgaaagctga ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc
     2941 ttactcctca atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt
     3001 cctgtaacaa ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc
     3061 atatcgtcgt ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat
     3121 cacactgact ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac
     3181 taagaacaaa ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata
     3241 tattctagag acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat
     3301 caacatgact gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta
     3361 atctattccc agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc
     3421 aaatagccaa accaagagca acaatggaac aacagcatac aataactcat aatttcctcg
     3481 tatcacagtc gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg
     3541 ataagcaaaa aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg
     3601 aagcatcaat tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc
     3661 tgcaaaagca aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa
     3721 aatcatagcc actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga
     3781 aacaaattta ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc
     3841 cacaaggatc aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg
     3901 cccatctaac tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat
     3961 actacttaaa ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct
     4021 aattgagaat gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt
     4081 tcatatgttt taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa
     4141 ggagggtatt ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg
     4201 gtacaccttt agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct
     4261 gctatcttag tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa
     4321 aagaactcaa aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg
     4381 tccaaaacca atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg
     4441 attacgtcac tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa
     4501 atgaacctgt gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa
     4561 aagaaaaacc aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac
     4621 ataaattttt acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt
     4681 caaaagcttt ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa
     4741 tcttctgcct tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc
     4801 ttcgatgatc ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat
     4861 caaatcgata ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt
     4921 gtaagcttca gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga
     4981 gttctccctt agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg
     5041 ttctttcaac atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg
     5101 attcgttcca cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat
     5161 actgtcacca ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat
     5221 cacaaagaaa cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc
     5281 acgaaaagca atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat
     5341 cgtgatacct aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa
     5401 tctcggataa gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg
     5461 gaacccagct tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac
     5521 gatatagctg tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa
     5581 ttgcagacgc aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat
     5641 ggcatcgaac ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata
     5701 gggacgttca tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg
     5761 actcgatagc tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat
     5821 ttgagtcaat gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat
     5881 ttgcttcatt ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg
     5941 ttgttctagt gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc
     6001 ttctttttct tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc
     6061 tggattgata cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg
     6121 aacacctgtt gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata
     6181 aaattctttc tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata
     6241 ctgtttcata gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact
     6301 gttgcgaata ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc
     6361 actcattggt gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct
     6421 cgctgttttc aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg
     6481 gctgaacaat gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac
     6541 catctgtttt cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa
     6601 attttttaat tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact
     6661 taatggccag atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc
     6721 gagatactga aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt
     6781 ttcgctaaaa tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg
     6841 atcggttttt gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa
     6901 tcgagcttgg tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat
     6961 cattaagtga cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa
     7021 cgaaattgtt tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc
     7081 tatgttaaaa acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt
     7141 agacatgggt tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg
     7201 atcaaacggc tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg
     7261 caattcatta cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa
     7321 aacaagaccg gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg
     7381 accagtggtt gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg
     7441 gattactcgt tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc
     7501 tgcagtatca actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa
     7561 tacgacagct gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc
     7621 gccccagaca actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa
     7681 tactttttta ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc
     7741 tacaccatta acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc
     7801 atttacttca ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt
     7861 ttggacttct gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt
     7921 aattcgtatc gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc
     7981 aaaaaagtct tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa
     8041 tgttttacgc gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt
     8101 tttctcttcg ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct
     8161 actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca
     8221 cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag
     8281 catattggat catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt
     8341 tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca
     8401 taaattccaa aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg
     8461 gtatttcttt gattttagga tctaattctt cttggtcata agcgattact gccttattaa
     8521 gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca
     8581 acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag
     8641 acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg
     8701 atacttccat cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc
     8761 cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg
     8821 ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt
     8881 gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt
     8941 ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt
     9001 cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg
     9061 catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac
     9121 gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc
     9181 ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat
     9241 aatggaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag
     9301 actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc
     9361 agttggttcc gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat
     9421 tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc
     9481 catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt
     9541 tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac
     9601 tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat
     9661 atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg
     9721 cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt
     9781 ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag
     9841 cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt
     9901 taatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac
     9961 ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg
    10021 ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa
    10081 ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc
    10141 tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt
    10201 ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta
    10261 atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc
    10321 aaaacgttca agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa
    10381 acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc
    10441 ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa
    10501 ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg
    10561 tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac
    10621 cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc
    10681 taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac
    10741 ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc
    10801 cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg
    10861 ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg
    10921 ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag
    10981 ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac
    11041 cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg
    11101 tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc
    11161 ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga
    11221 tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac
    11281 tgatgcttct acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat
    11341 atattgacgt tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc
    11401 aacatattga tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa
    11461 ttcttgctgt gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc
    11521 tgctgctttc atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc
    11581 tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa
    11641 aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg
    11701 atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga
    11761 ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg
    11821 tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac
    11881 atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc
    11941 ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt
    12001 gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac
    12061 accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc
    12121 tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa
    12181 ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt
    12241 ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact
    12301 attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc
    12361 aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac
    12421 ccagcggata gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt
    12481 gacgctattt tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc
    12541 aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt
    12601 ccgccgcagc ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat
    12661 catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca
    12721 atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat
    12781 gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga
    12841 atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata
    12901 ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca
    12961 aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga
    13021 tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat
    13081 actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg
    13141 ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca
    13201 aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt
    13261 ccattttcca cattcctttt atggatattt tttcgatact tccttattat actggttttt
    13321 tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg
    13381 acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg
    13441 gctttccata tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt
    13501 ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa
    13561 atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg
    13621 tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca
    13681 gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa
    13741 tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc
    13801 aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg
    13861 agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt
    13921 attccaagca gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa
    13981 agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca
    14041 ttgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa
    14101 gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt
    14161 gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat
    14221 ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa
    14281 acctgcatag aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg
    14341 taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt
    14401 ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt
    14461 atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc
    14521 atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat
    14581 ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc
    14641 tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg
    14701 atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata
    14761 tgaccagata ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc
    14821 aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata
    14881 ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt
    14941 attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga
    15001 ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag
    15061 cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg
    15121 agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc
    15181 tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc
    15241 attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga
    15301 gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat
    15361 cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact
    15421 agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac
    15481 tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta
    15541 agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca
    15601 agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg
    15661 tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa
    15721 aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg
    15781 tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa
    15841 aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg
    15901 gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt
    15961 agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc
    16021 cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt
    16081 catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt
    16141 tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga
    16201 tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa
    16261 attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt
    16321 ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg
    16381 gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa
    16441 aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag
    16501 ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga
    16561 agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg
    16621 actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga
    16681 tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc
    16741 ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc
    16801 accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa
    16861 aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta
    16921 gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg
    16981 cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat
    17041 ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc
    17101 ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc
    17161 tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa
    17221 tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg
    17281 cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct
    17341 ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca
    17401 tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg
    17461 tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag
    17521 atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg
    17581 tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat
    17641 aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga
    17701 acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca
    17761 acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta
    17821 tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct
    17881 tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc
    17941 tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa
    18001 cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg
    18061 gctgttacct tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac
    18121 tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca
    18181 tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg
    18241 tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa
    18301 tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct
    18361 tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga
    18421 ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt
    18481 ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac
    18541 agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca
    18601 gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct
    18661 tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc
    18721 gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca
    18781 cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta
    18841 ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc
    18901 tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat
    18961 gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc
    19021 gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg
    19081 ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat
    19141 tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca
    19201 tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg
    19261 ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa
    19321 ggacattaca cagaataaat cgttttataa caaag
//
LOCUS       DAZKNO010000041        19215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000041 DAZKNO010000000
VERSION     DAZKNO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.041"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(193..1884)
                     /locus_tag="ACG8MY_001072"
     CDS             complement(193..1884)
                     /locus_tag="ACG8MY_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFQ0430288.1"
                     /translation="MLFNQHVDQYIRKWKSGELILNEKRIQLLKLIEKEILPRDDIYY
                     FDEEQINNYIEFSQAWYFELDEWEKFISAFIFLFYKEDDEVVFDEFVINMGRGGGKNG
                     FISTLANYFVSSLHGIDYYDVSIVANSEKQAKRSFQECYRVINKKGNEDLKEEFEAYK
                     SSLTGLETQSVFEYKTSNASSQDGGREGAVIYDEYHEMENTDIVDVFSGGLGKVDHGR
                     QFFIGTKGFVREGYFDIKYRECEDILNGLSEFKGVFPYICELDAIEEMDDPKNWPKAN
                     PALQPPLNKRGKRLFNEVMKKYKKLAKEPSGRSAFVTKRMNFLEDNMENSVASREEIM
                     ATNRPFFELDTVPIGSLDFGSVRDFATCGLLFKNRKKSEYAFKSFTFAMKNFCDVHYG
                     YSLKEQLVGSEKRAPIKTWEKMGLMKVIDEPSLNPKYIVDWFIQAREEYGVRVIVVDN
                     YKADILGPMLEKEGFEVVRLKRPSSLHPLLAPRIEDGFANHKFIFGDNPLMRWFTNNV
                     YVKETQAGKQFLKKEEVKRKTDGFQAFVHALYKATELDEQIDYDDAFDMLDEIEF"
     gene            complement(1887..2405)
                     /locus_tag="ACG8MY_001073"
     CDS             complement(1887..2405)
                     /locus_tag="ACG8MY_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430289.1"
                     /translation="MKQKRIEKGLTQADVATKSGISSRYYGSIENGKNSPSIETLNSI
                     SIVIGCSLHFLLNDRMDDMEKRVELEKKRLQKIFDKIPHDKLNLVEGLIIQAARLRVL
                     LDDNWKDIIENGEYEKFKQSENQIAYDRKRPIVENYDNRDKTYQAIVKQLTDLLPQGT
                     RADKKSKLLGRK"
     gene            complement(2609..2866)
                     /locus_tag="ACG8MY_001074"
     CDS             complement(2609..2866)
                     /locus_tag="ACG8MY_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430290.1"
                     /translation="MVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEETTTVEDI
                     SINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            complement(2860..3171)
                     /locus_tag="ACG8MY_001075"
     CDS             complement(2860..3171)
                     /locus_tag="ACG8MY_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0430291.1"
                     /translation="MIDVSTKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            complement(3172..3744)
                     /locus_tag="ACG8MY_001076"
     CDS             complement(3172..3744)
                     /locus_tag="ACG8MY_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430292.1"
                     /translation="MKLKDYIREGYNIVTTPNLAYKIQEDYPNVLVFTDRALDAIPIG
                     KLLVDRYYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWHTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            complement(3785..4069)
                     /locus_tag="ACG8MY_001077"
     CDS             complement(3785..4069)
                     /locus_tag="ACG8MY_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430293.1"
                     /translation="MNDNYDYIKLIEKIRAEKDMDELANLFMNIISLVGLKMDEVAAL
                     NYFIAEQTIRAEHNAKFLKDRLDLDVKGLGVEGIFKVQEALVNVYVEKMQ"
     gene            complement(4066..4293)
                     /locus_tag="ACG8MY_001078"
     CDS             complement(4066..4293)
                     /locus_tag="ACG8MY_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430294.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIIEMTREAT
                     PNEIDKYNLRYCGHGYWKDEYIQQNIERYIK"
     gene            complement(4300..4512)
                     /locus_tag="ACG8MY_001079"
     CDS             complement(4300..4512)
                     /locus_tag="ACG8MY_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430295.1"
                     /translation="MNEKQLSELFKLNESNQTAEATFYEAQKSLALIAKQAKYFYDQL
                     ILQGFNEGQAMEFMMRTFSASNQQKE"
     gene            4871..5119
                     /locus_tag="ACG8MY_001080"
     CDS             4871..5119
                     /locus_tag="ACG8MY_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430296.1"
                     /translation="MKITAAITKEQGVTFTVVVVKNGVISSSHREQVRSSIPRNFPRP
                     IILAEQSHGKMKYHGRTDIVKFLSNVPYQALPWKDYTL"
     gene            complement(5073..5333)
                     /locus_tag="ACG8MY_001081"
     CDS             complement(5073..5333)
                     /locus_tag="ACG8MY_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430297.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIAIVNKAE
                     SENSSFFVKANWNIDLPEGDINNPVAEPIKYNPSKAKLDKVH"
     gene            complement(5595..6011)
                     /locus_tag="ACG8MY_001082"
     CDS             complement(5595..6011)
                     /locus_tag="ACG8MY_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0430298.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            complement(6271..6564)
                     /locus_tag="ACG8MY_001083"
     CDS             complement(6271..6564)
                     /locus_tag="ACG8MY_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430299.1"
                     /translation="MELKIDKVTFEEPAFQEMDERIAELVEKKQDELIALLEKKYQFP
                     KYMNKMQAAKYLGVSYNTMMDKYVPAGLKMGIVNGVIRISKEECDRFMKEHQK"
     gene            complement(6548..6853)
                     /locus_tag="ACG8MY_001084"
     CDS             complement(6548..6853)
                     /locus_tag="ACG8MY_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430300.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVINNGIKN"
     gene            complement(6850..7053)
                     /locus_tag="ACG8MY_001085"
     CDS             complement(6850..7053)
                     /locus_tag="ACG8MY_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0430301.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            complement(7060..7290)
                     /locus_tag="ACG8MY_001086"
     CDS             complement(7060..7290)
                     /locus_tag="ACG8MY_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430302.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            complement(7287..7625)
                     /locus_tag="ACG8MY_001087"
     CDS             complement(7287..7625)
                     /locus_tag="ACG8MY_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430303.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            complement(7622..8020)
                     /locus_tag="ACG8MY_001088"
     CDS             complement(7622..8020)
                     /locus_tag="ACG8MY_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFQ0430304.1"
                     /translation="MIPKFRAWDKRENTMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLG
                     HRNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            complement(8017..8262)
                     /locus_tag="ACG8MY_001089"
     CDS             complement(8017..8262)
                     /locus_tag="ACG8MY_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430305.1"
                     /translation="MIKKLIQFSMDLYDIESGATVSVESDHLIISFADERQIIIWVVD
                     DMLYPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSK"
     gene            complement(8222..8578)
                     /locus_tag="ACG8MY_001090"
     CDS             complement(8222..8578)
                     /locus_tag="ACG8MY_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFQ0430306.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKAKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKAHSIQHGFI"
     gene            complement(8717..9532)
                     /locus_tag="ACG8MY_001091"
     CDS             complement(8717..9532)
                     /locus_tag="ACG8MY_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFQ0430307.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            complement(9543..9761)
                     /locus_tag="ACG8MY_001092"
     CDS             complement(9543..9761)
                     /locus_tag="ACG8MY_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HFQ0430308.1"
                     /translation="MIGLTIFWCAIGGLLAFLGYYLICESRKGRKYTVTGIVLLIIGI
                     LVAVGAELNLYVNGTQEDLVRFMFWMKN"
     gene            complement(9758..10069)
                     /locus_tag="ACG8MY_001093"
     CDS             complement(9758..10069)
                     /locus_tag="ACG8MY_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0430309.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            complement(10066..10368)
                     /locus_tag="ACG8MY_001094"
     CDS             complement(10066..10368)
                     /locus_tag="ACG8MY_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430310.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVTEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEKK"
     gene            complement(10361..10525)
                     /locus_tag="ACG8MY_001095"
     CDS             complement(10361..10525)
                     /locus_tag="ACG8MY_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430311.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            complement(10530..10799)
                     /locus_tag="ACG8MY_001096"
     CDS             complement(10530..10799)
                     /locus_tag="ACG8MY_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430312.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWCKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            complement(10811..11662)
                     /locus_tag="ACG8MY_001097"
     CDS             complement(10811..11662)
                     /locus_tag="ACG8MY_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0430313.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            complement(11669..12355)
                     /locus_tag="ACG8MY_001098"
     CDS             complement(11669..12355)
                     /locus_tag="ACG8MY_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0430314.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            complement(12361..13032)
                     /locus_tag="ACG8MY_001099"
     CDS             complement(12361..13032)
                     /locus_tag="ACG8MY_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0430315.1"
                     /translation="MSENKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(13025..13366)
                     /locus_tag="ACG8MY_001100"
                     /pseudo
     CDS             complement(13025..13366)
                     /locus_tag="ACG8MY_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13544..14242)
                     /locus_tag="ACG8MY_001101"
     CDS             complement(13544..14242)
                     /locus_tag="ACG8MY_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0430316.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            14329..14799
                     /locus_tag="ACG8MY_001102"
     CDS             14329..14799
                     /locus_tag="ACG8MY_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430317.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(14842..15021)
                     /locus_tag="ACG8MY_001103"
     CDS             complement(14842..15021)
                     /locus_tag="ACG8MY_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430318.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(15008..15346)
                     /locus_tag="ACG8MY_001104"
     CDS             complement(15008..15346)
                     /locus_tag="ACG8MY_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0430319.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(15359..15544)
                     /locus_tag="ACG8MY_001105"
     CDS             complement(15359..15544)
                     /locus_tag="ACG8MY_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430320.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQEHIETVKKILGIQ"
     gene            15847..16221
                     /locus_tag="ACG8MY_001106"
     CDS             15847..16221
                     /locus_tag="ACG8MY_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0430321.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            16226..16654
                     /locus_tag="ACG8MY_001107"
     CDS             16226..16654
                     /locus_tag="ACG8MY_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0430322.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            16704..17357
                     /locus_tag="ACG8MY_001108"
     CDS             16704..17357
                     /locus_tag="ACG8MY_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430323.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETNISKTKET
                     TEISSSTSKSTSKTDSSSTVTSSNQATAKPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNTEAQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            17494..18144
                     /locus_tag="ACG8MY_001109"
     CDS             17494..18144
                     /locus_tag="ACG8MY_001109"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFQ0430324.1"
                     /translation="MKKNLILGFICTSILTLGACSSPSNNATNSETQKTINSLKKENE
                     ELKKQIASQSNDTEENSESIDNASKEKTLPVVGILEYTISSIKTEQVTNDEDNFTNAE
                     YNFSGIENFPEKYYRTTISYKLKNKATSPFSLSSYQASLIDGDGMEYSRTAGEYFLYD
                     ENSNSLVQPETTTSGVFYLLSKNKPNLSTFKLNASKQFTDDFTAEPIGEAGTTEYK"
     gene            18254..>19215
                     /locus_tag="ACG8MY_001110"
     CDS             18254..>19215
                     /locus_tag="ACG8MY_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0430325.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
BASE COUNT         5649 a         3952 c         2892 g         6722 t
ORIGIN      
        1 aaaattgaaa taattagttt agctctttct aaaaccaaat tctttgaaac tacactgtca
       61 tttggaataa taatttctga tagttttatt gccacaaatg taaaagctaa aatccccaac
      121 atgtaaaata cccaagatat gttttttcct tttatcctca tgaaagcacc cctttgtaaa
      181 gaaaagtata ccttaaaatt caatctcatc caacatatca aaagcatcat cgtaatcaat
      241 ctgttcatct aattctgtag ctttgtacaa tgcgtgtaca aatgcttgga acccatcagt
      301 tttacgcttg acttcctctt tttttaagaa ttgtttgcct gcttgcgttt cctttacgta
      361 cacattatta gtaaaccaac gcattaacgg attatctcca aaaataaatt tgtgatttgc
      421 aaatccatct tctatacgag gagctaataa tggatgtaaa ctagatggtc tttttagtcg
      481 aactacttca aatccttctt tttcaagcat aggccctaaa atatctgcct tatagttatc
      541 cacgacaatt actctcactc catattcttc tctagcttga ataaaccaat caacaatata
      601 cttaggattt aaagatggct cgtcaataac tttcatcagc cccatttttt cccaagtttt
      661 tattggtgcc cgtttttcac tgccaaccaa ttgctctttc aacgagtatc cataatgaac
      721 gtcgcaaaaa tttttcattg caaacgtaaa tgatttaaac gcgtattccg atttctttcg
      781 atttttaaat agcaaaccgc aagtagcaaa atctcgaaca ctaccgaaat cgagagatcc
      841 aatcggtacc gtatctagtt caaaaaatgg acgatttgtt gccattattt cttctcttga
      901 agcaacagag ttttccatat tgtcttcaag aaaattcatt cgtttagtaa caaatgccga
      961 gcgtccagaa ggttctttag ctaacttttt gtactttttc atgacttcgt taaacagacg
     1021 tttcccacgt ttattcaacg gtggttgcag tgccgggttt gctttcggcc aatttttggg
     1081 gtcatccatt tcttcaatgg catccagttc gcatatgtac ggaaatacac ccttgaactc
     1141 tgacaaacca tttaaaatat cttcacactc tctatattta atatcaaagt aaccttcacg
     1201 aacaaacccc ttagtaccaa tgaaaaattg ccgtccgtga tcaactttcc ccagtccacc
     1261 agaaaacaca tctacaatat ctgtgttttc catttcgtgg tattcgtcgt atattactgc
     1321 accttctcga ccgccatctt gagagcttgc attactagtt ttgtactcaa aaacagactg
     1381 tgtctctaaa ccggttagac tgcttttata cgcttcaaac tcttctttta aatcttcatt
     1441 tcctttttta ttgattactc gatagcattc ttgaaaactt ctttttgctt gtttctcact
     1501 attagctaca attgaaacat catagtaatc aatcccgtga agcgaactga caaaataatt
     1561 tgccaatgtt gaaataaagc catttttacc cccgccacgc cccatattaa taacaaattc
     1621 atcaaaaaca acttcgtcat cttctttata aaacaaaaaa atgaatgcag aaataaattt
     1681 ctcccattca tctaactcaa aataccatgc ttgactaaac tcgatataat tatttatttg
     1741 ctcctcgtcg aagtaataaa tgtcgtcgcg aggtaatatt tctttttcta ttagttttaa
     1801 aagttgtatc cttttttcgt taaggattaa ctcaccagat ttccatttac gaatatattg
     1861 atcaacgtgc tggttaaaaa gcatttttat ttcctaccga gtaacttact tttcttgtcc
     1921 gctcttgtgc cttgcggtaa taaatcggtg agttgtttga cgatggcttg ataagtttta
     1981 tctcgattat cataattttc aactatagga cgctttctat cataagcaat ttggttctcg
     2041 ctttgtttaa atttctcgta ctccccgttt tcaatgatgt ccttccagtt atcatccaac
     2101 aagacacgta accttgctgc ctgaatgatc agcccttcaa ctaaattaag tttgtcatga
     2161 ggaattttat cgaatatctt ctgcagacgt ttcttttcta gctctacgcg tttttccatg
     2221 tcgtccatgc gatcattgag taagaaatgt aaactgcatc caatgacgat ggaaattgag
     2281 tttaacgttt cgatactagg ggaatttttc ccattttcaa tacttccata atacctacta
     2341 gaaataccac ttttcgttgc tacatcagcc tgtgtcaatc ctttttcaat tcttttttgt
     2401 ttcattctcg tgccaaccaa cttagaattc aaaccaatca ccttccttct atatttacta
     2461 aaggggcggc ggagggggga ggggtaaaat aaatacgttg catttgcaga attaacccca
     2521 tccaccggtt cccaaaaatt ttatttcttc tcaaaatctt ttgaccgggg gtatttcact
     2581 ctatatatct tcgctttata ctgatttatc agccgtcttg ctcgttgctt tctacaatat
     2641 gtgcaaatgg actcaaggta taatcaatgt ctacattaat attaaatgtc cctgttactt
     2701 cattagcgtt acctattagt ctattgttga tagatatatc ctctacagtt gttgtttctt
     2761 caatgagctg actcttaaat agaaccaact tatcaatagc cttatcaacc atagcgattg
     2821 cttctgctcg tttgacatca tctctgtttg ctgtttttct taccaccatt catcatccca
     2881 ctttcttttc ttttgttggc cacgatattt catacggttg tgccttttgt tatggcaatc
     2941 tttgcataag gttcttaggt tgtttatatc tagagctaaa tccggatgat actccagctc
     3001 ctttatatga tccacttcaa gaattgaatc atgttgagtt gttagtctac cctctgcttt
     3061 acaccattgg cactcataat gatctcgctc taagcattga cttctaagtt tcctccactc
     3121 agttgatcca tagaacctag ctcttgcttg tttagttgaa acatcaatca tttacatatc
     3181 tctcttgtgc ccaatgattt ttctatctaa gtacttgtta ttatgtgaat cataatattc
     3241 aatagtgatg tcgttagctc cttttggtac ttggcattca tattcttccg tatgccaatg
     3301 ataagtaata tcgacaagac ctatcggcat ctcatccatt cgttgacctt tataataaac
     3361 ttctggtact gagtcagtat ctcttagttt gatttctaga aggttcgtat tacttccatc
     3421 atctaacgtc aattcagcaa tcctcttttt gatctgttga attacttttc ttcctgtctc
     3481 gcctgtcatt ggtactaccg tctcatatcc agtaaactcg caagtaaagc gttcaatagc
     3541 ataagcgttt tgaagtggct tagcactaag aacagctgga ttgttgctat aataacgatc
     3601 tactaataac ttccctatag gaatggcatc taaagctcta tcagtaaaca ctaaaacatt
     3661 gggataatct tcttgaattt tgtaagctag atttggtgta gttacaatgt tatacccttc
     3721 cctgatataa tcttttaatt tcatagtttc cctcctgaaa acatccaata attacttgtt
     3781 aatgctattg catcttttcg acataaacat tcactaaggc ttcttgcact ttgaatatcc
     3841 cttcaacgcc taatcctttt acatctagat ctaacctatc tttcaagaac ttagcattgt
     3901 gctctgctct aattgtttgc tcagcgatga aataatttag tgccgctact tcatccatct
     3961 ttaaccccac caaactaatg atgttcatga ataagttagc tagttcgtcc atatcttttt
     4021 ctgctcttat cttttcaatc aacttgatgt aatcatagtt atcattcatt tgatgtacct
     4081 ctcaatgttt tgttgaatat attcgtcttt ccaatatcca tggccacagt aacgaagatt
     4141 gtacttatcg atctcatttg gtgtagcttc tctggtcatt tcaatgatgg agtacttctt
     4201 tttgatttgt acagaacgca caacacgcac tgaacaatca tcaatggttc gaggatattc
     4261 attagttagc gatatatacc agtagtttct cattatgtat cactctttct gttggttact
     4321 ggcagaaaag gttcgcatca taaattccat agcctgtcct tcattaaacc cttgtagaat
     4381 aagctggtca tagaaatact tagcttgttt tgcgattaac gctaagcttt tttgagcttc
     4441 atagaatgtt gcctctgcag tttgatttga ttcattcaat ttaaataact cgcttaattg
     4501 tttttcattc atagataaaa ctcctttcaa aataaaaaga ccactcaaag agtgatctaa
     4561 tatgtaaaaa ctacacctca taaacgagat gcagttatag ttctatcaag caacctacac
     4621 cgattccatc gattactatc gacctcgcct tgctcgtgta cttggagcgc ccatttccaa
     4681 ccctcggttg ccaaagtcac tggcaatgaa tcgaacattg catggttgcc gaagcattga
     4741 cctagcacgc atgcttagcg tctacccttt ccgccacagt gacaaattaa tattgtgaaa
     4801 ataaatacta agcgtataat tttatttatc agcgagtggt ccgctgaaat aaattatagg
     4861 tggtgaatat atgaaaataa ctgctgctat taccaaagaa cagggcgtaa cttttacagt
     4921 tgttgttgtg aaaaacggtg tgatctcttc ttctcataga gaacaagtta gaagtagcat
     4981 cccaagaaat ttcccaagac ctattatctt agctgaacag tcgcatggga aaatgaagta
     5041 tcacggtaga acagatatcg taaaatttct ttctaatgta ccttatcaag ctttgccttg
     5101 gaaggattat actttatagg ttctgctacc ggattgttga tatccccttc tggtaaatca
     5161 atgttccaat ttgcttttac aaaaaaacta gaattttcac tttcagcttt attcacaata
     5221 gcaatttcat ctaaagtttc ttgtaactgt tcgacttggt cactagcctt gttgagcagt
     5281 tcttttagtt cagtcaaatc taatttgaca gttactttga tttctttatc cattcgtttt
     5341 ccctccaata cataaattaa tagacagcaa cggatgatag ataataagaa caatttagaa
     5401 ggagttaaaa ttcacatcct tattcttaat atttccgctg ctgtctatcg aagcttaatt
     5461 gtgaaacaat aataaaacga tgttcctttt attattattt tgtcttagac ctatcactaa
     5521 tctttcgaca ctatcataat atcacgttaa accactcaaa aaccctacac tatccctaca
     5581 aaaaccctac aaaatcaacg atactgaact aatacgcctt ttttgtatgc ttcagcaaat
     5641 tcgatcagcg cgatagattt cagcttctct acattcttct ctccgtatcc tcgtatcaat
     5701 tgccctattt cataattaga gtgcttgttt acgtcacaaa agctataata gagtatctga
     5761 cggctaatca gactaagagc catcaaagcc gctagaatcg catctctctc tgcttctata
     5821 tccatcatct gaataatcgc gtcctctgcc ttattgccgt gcttcggtgc cttcggcata
     5881 tccgtaataa tcggcgactt aatatctatc aaagagcgac ctgccatccg ctctaagcgt
     5941 cgaaaattct tcagcacatc tctcgcattg catcttgtct gtttgaaatc tacctctcgt
     6001 aacaattgca tcaagtcaaa ccgctccttt atgtgatata ataaacttgt tgggtttatt
     6061 acatcagtcg gagcgatccg gcttttttta tttgccgagt tacacttctc gacttagcat
     6121 gctggtgtgt caaatacttc aatatatgct tcttctgttt ttattgtctt tttcattccg
     6181 ccattcctca acctccgcaa ttttgttata attaactgtc aggtaaatta taattgtgcg
     6241 tttagctaag gggctaaacg tttttttatt ttatttttga tgttctttca taaaacgatc
     6301 acattcttct ttgctgatac gaataacgcc gttaacgata cccattttca aaccagctgg
     6361 tacgtactta tccatcatcg tgttgtaact tacaccaaga tattttgctg cttgcatttt
     6421 gttcatatat ttcggaaatt ggtatttctt ttctagcaaa gcaatcaatt catcttgttt
     6481 tttctcgacc aattctgcaa ttcgttcatc catttcttga aatgctggtt cttcaaacgt
     6541 taccttgtca atttttaatt ccattgttta taacctccat atccaccaat ctcaccactg
     6601 ctaaattctc tttgcttttc gctaaccact tatcgcattc cattgtgttt tcaatacgaa
     6661 tgatcgctga gtgattatag agatgctcta catatccacg aaatggatag atgaaccctt
     6721 ctgcttcgca gcgaaccatg tcaccgactt taatttctga ttcctcttgc tttataggat
     6781 tcttagttgg tagatccatc atcaaaccac caattccata agcatcagtg taaaatccgt
     6841 cttttagttt catctttctg cctcccattt acgatcatca tttaatatcg aaatcccaaa
     6901 cttacgaata gtctcactcg catcagcaac aaactgactt gccactttat atgtttcttc
     6961 tgctgaaatt ccatattctt tttcaaactt tgtctttagt acattcagtt cctgttttct
     7021 tagttttgct actctgcggt gtctggtgtt cattgtcaat caactcccta atctgaaagt
     7081 gtcgtttata cttgatcgaa attctttcaa gtggttctct accacagaat cagtcacgtt
     7141 aaaacgatca attaatactg gagctgccat atctttcaaa taactttgtc tgatgactaa
     7201 ttcagtacca tcaggaagtt ttttattaac ctcccgacca ttgataattg cttgaatgcc
     7261 cgcttcactt agtggtattt cgtatttcat tccgcttcct ccttcaacca taaatttgga
     7321 aaatcatcgg catatatctc tattgcgcca aataacttga tcgctgattt tgattttcca
     7381 tgcatgtcag gactgattaa acgatcataa taatcctgac tgattgtttt tcccttgtac
     7441 tgataacgtg tagccgcagt gtaatatact gctgtcttgc tcagataaag atattttgct
     7501 aaatcagccg atatccgtaa caaaatgatt ctattcttgc ttttaattcc acaatttagc
     7561 aatgctggga cattcccatt ttcatctttt aaagaatcga tgtagtcagt agctaagata
     7621 atcactctac ctccaataac tcgctattct cgtatatatt tccgatgact tcatgagcag
     7681 ttgctacatc ataactttcg ccagtattcc tatggcctaa tctataatca cttttcttta
     7741 gtacaactac aaagccggct tcttgttgaa gatacgcaac atatccaatc gctccttttg
     7801 atgtaattac tacatcccct tcaaatatct ccacaccatt cttatctttc attcctgtgg
     7861 attgcatgag gtcgatgttt cgcacgtgat atgatttcaa ttcggtagga tttatccaat
     7921 attcaataaa gtttgttttg ccgtttttag taaaatgtaa gacagctaca tctctcattg
     7981 tgttttctcg cttatcccac gctctaaact tcggtatcat ttgctgtcct cctcgtattt
     8041 ttcaatcaat tccattactt ttttcactat ttcaaactca accgcttttg attcttcaaa
     8101 atcatgtaca atttctggat atagcatatc gtcaactacc catatgataa tttgacgttc
     8161 gtcagcaaaa cttatgatta aatgatcaga ctccacagat actgttgctc ctgattcgat
     8221 atcatataaa tccatgctga attgaatgag cttttttatc attcgctgtc ctcctcaaat
     8281 actcttctaa tatttcttta tacttctcta caaatttgaa acgatcttga tgaagtttct
     8341 tgctccaatt tgtttgccga tccagctcac gcatctgatc gaaccctttt tgaatttcgt
     8401 tgtaataaaa ttcaatgttt gctgctgctt tccaatgcct gctacttcgc actcctactc
     8461 ctgtttcagc catttccaac ttaactaatt ccgctcgttc ttttgctttt ttatctttct
     8521 gaatctttgc catgattttc ttgaggatga tatcactgta ttgtgtaatg agatccatta
     8581 tttctcctcc taaaatttca tttcatcgtt gttgtcatct tctttgtcat cagtttggga
     8641 aaggagcgca tacacgagat atgctgctcc tactaaacct aagaacaaga gaattttagc
     8701 cataaagaat cccatattat ttgcctgctt catccgtaac tacagtatct gctccattta
     8761 ctgttaccca tccatgcttt ttgcgagctt gagcttcttc ataacgaatc aaattgtctg
     8821 ttactgattc ggcgactttg cgattagatt ctgcctctgc ttctgcagct tttgttttct
     8881 tgtaagcttc actatcagct tgagtttttg cagtttctgc atctagcttc gctttttcat
     8941 tttcttgacc agctcgaatg atcgcatcga ttgatttttg tgtttcttta tcgacatctg
     9001 gaacaccaag tgttacgtct tcgacttcaa accctttaga ttcaactgat ttagcaaagt
     9061 tcgttaatac ctcagcttca actttagagg aatctcctga aaggacatca agcagactat
     9121 atttcgcata aacttcacga gcgacctttt gaagcttaga ttttaaccat ccactttcga
     9181 tatcttccga agtgatattc ccaaattcct tgtacatttt tgctgcttta gttgaatcaa
     9241 ctttgtagtc atatttgata tcaatcgttg tctttttgcc gtcgcttgta gatactgaaa
     9301 tattttttga ttggatagtt tgcaagcgaa ttggatattg gatcactttg tcaatcccta
     9361 caaatttcac accttgcgtc agtgcttcat ctttgatacc gccgttcatt gaatagcgca
     9421 cacccacata tccattatcg attttttcga agaacttaaa tcctccgata actccaatac
     9481 ctacgattac tgctcctgct acacctaatt tgattaattt gttttcgttc atttttttcc
     9541 tcctaattct tcatccaaaa cataaatcgc accaaatctt cttgtgtacc atttacataa
     9601 agattcagtt ctgctccaac tgcaactaga attccgataa ttaacaggac aatacctgtc
     9661 actgtatatt ttcttccttt tctactttca cagatgaggt agtatcctag aaatgctagt
     9721 aaaccaccaa tcgcacacca aaatattgtt aaacctatca tttgctgtcc tccaaatcac
     9781 tcgacttcac gaatacacca tctaccattt ttcctgtgcg ccctttgatt tcgttgtatg
     9841 ccatttctaa acactcttgt acgtttgtcc ctttttgcat ggaaaggata atcaacgtga
     9901 cgattacgtc tcctacgcta tctttaaata gctcatcatt acttcttgcc attgctgaag
     9961 cgatttcccc gaattcctca gctactttca aaaactgtgc ttttggatct gcttgatcca
    10021 gtcccttgtc tttagcccac tgctctactt ttgtgattag ttcgttcatt ttttctcctc
    10081 cacataccta aattgtcgtc cttttgaatc aatccataag ctccttgctc tatcccagat
    10141 gatgtttttg ctcagaccag taatttcaga taactgttca gcagtacctg ttactagaat
    10201 tcgatcacca tgccaaattg caatctttct cggcgttctc cgtttgggct tttcagtcca
    10261 cattgattta ccgagctttt ggacttctgt aactatttct ttgtcttctt gccaattctc
    10321 agaatgtgtc agttcgatga ttcgtttcat tgccgctttc ttatccatcc cgacgttccc
    10381 ctttcaataa tttgagtact tgatcaagtg cgctctcacg tccaccatgg aacgtgttga
    10441 gccacttgtc ttcgtacgag gcgctttgtc ttaaagcttc ttgatgcatt agttcgatct
    10501 gtgctgtaaa tgtttttaga tccatctgat tacacctgct caagttcact aagatgtttt
    10561 tgcaatcctt taacgcaatc aacaaatagt aattttgtat aagctaaatt tcttaattgt
    10621 gttgtatcga tatagagtgc gaaatagtat ctgagtttac accaactcga acgatcatta
    10681 ttaattcctt cgattccagc ttcttcgagt tgatcatata cgtctctcag gatttctatt
    10741 tcctcaccag ttttatactt tgctatttca ttaattagtt ctagataatc gattttcaat
    10801 tttccacctc ttaaaatggt gcttttgatt gtctattagc tcgttctagc gcttttttct
    10861 tgagataggc ttcttggtcg attgcccatt caggaagctt ctctcgtctt cctgtgcgct
    10921 tgtatgcacc acttgcattc ttaggctcac ttttttcttt ccttgcccaa cttcgaatag
    10981 ttgccaaata gtttttataa gtcttaccag atgattcaca atactcagat agccgttcta
    11041 ttcgttcttg atagtcatta gggaattcta ttttgagttt ctccatctgc tcatctgaca
    11101 aaagaacatt tttatactct ccgtatttat gacggacggg cttagccttc gattttttcg
    11161 aaggcgttac attctctata tctttctcta actctatatc tttctctaac tctatctcta
    11221 actctggtgt agttttgtcc ggacatttgt ccgacatttg tccacttgtt attaaattct
    11281 gttttgcttc ctcaatttct tttctgtatt ttctcttcct atcagcttca gttgaagatt
    11341 tcccaataaa actttgtata tcagacatat aaattgctcc gttatctaat acctcaataa
    11401 gttgtaaatc acgaaatatt tgtactgctt tttctacaac tcccacagag tgtcttgtaa
    11461 tagtcgcaag cattgtagaa ttgaatggaa tcctgtcatt aaacattagt ttgccttcat
    11521 gtttcagact tcttaaataa agtttgagaa gaatattaga ataaatgtat ccatctggca
    11581 tactttctaa aagaaccatc tcgtcgctat cgaaaaagtt ctcttttagt tttaaatagt
    11641 agtatcgttt gttgtcagcc aatatttttt atcctcctat tctaagtttc ttaattgttt
    11701 cctggtttaa cttgatccct ttgatttgat atttattttt gaaattgatc acacctatct
    11761 tgtgtttctc cgtgtgatgg attctgcaga gtgctgcaaa tgtgtactct gaatgatcaa
    11821 cttctttgcg ctttcgtctt cctagcgctt tgtcaaagtg atcgatatca gctcctgttt
    11881 tgccacagat gcaacagact ctttttgtga tgcatttgta gaagtaatat tcttgattcg
    11941 ctggtaaaat ctcatagcct tctttgaaag gaatatgatg ttcaaagatg aaatctaaga
    12001 tgatatttgc taagacgtta gcatcactca cagtcgtatt cgattcatct ttgaggctta
    12061 ttttgcgccc tgtgacacct tcaaagcgga agtaaaagaa ttccttccag aagtccgttg
    12121 gcatgcctgt atcgatgaaa atatcgccta tgagtgcata gatgaagttt cgttgctgta
    12181 ctgtgaaacg tctaggatca ataaaacgaa tttcaataac tcgatcacca tcgtagccgt
    12241 catacatcgt ctttagtcga tcaatgttca cttcctcatt gatggttgca cctatgtctt
    12301 ttcctttgaa ctttttcaga accgctgaat atgaatcgat taatggttta aacactcata
    12361 tcacttctta tctaattctt ttctcttagc tgctattgct cgctccatca aggcacattg
    12421 ctcatagctt aactgttcaa tagtttcaac gttatcagct aagagcccta atttatctgt
    12481 ctgctcatta acatattcga ttaaggtttt ggtcatatct ttacccatct gctcattgaa
    12541 agcttctaga atcgtctcta gcatgtttaa tttcttcgta tcgattctag gtggtgttgg
    12601 aatatcttcc ccttgaaata catataatcc cagtccgtgt agagccaatg ctttcacaaa
    12661 gcatcgcttc aatgagttat tgatttgcat tgcatttggt ttaacaactg gttggtttcg
    12721 ataatctaaa acaggaaata actcggtttc cgtgtgtcct ttaaccgtta ctgagacaga
    12781 tacataagtc cctgtttcat ccataagaaa aggtttgtat tcctcaacaa gaaagtcttg
    12841 atgagttcca gaaacaactc tgtagtgttt gtactcatta atagttactg ttgcctgtgg
    12901 atcattcttt ttcataatct cccatgcgtg agcccaagat aaataatcaa aatttccttt
    12961 tttcttgaga attttattta acttgcgact aaaaagtttt tcaaaattcg ttgtcccttt
    13021 gttttcactc atcaaattct gcctccaatt cagcaatgta tttcttacct ggtccgtaat
    13081 aagagatatc gatcaagtta tccctttcgt actcttctaa tgcatcaatc aagccatctt
    13141 cgatgacgta aatatattca ggtttattcg aatgcttcga taaatggata agataaacat
    13201 gatctcaaat agttacaagg ttacctaaat catcttgatc acaagctagt tcttcattcg
    13261 tcaaaagatt tcgcctgatt tttcgaccac ttgtttcctc aattttcggc ttgccccaat
    13321 caggatcaat caaatattga tctagagtgg aaagttcttt ttccatatgc taaaatctcc
    13381 ttatgatgtg ttttctttgt gactctttgc ttgccggcgg agtcactttt ttatttgttg
    13441 ccatgctttt tgcttgtcaa tatgttgttg gcttaggatg attggtttat agtatttcca
    13501 ccagcaatta gcaattgccg tccctattct tagcgcttca gctctattca ttgtcatcac
    13561 caaaaagtct ctgttgtctg ttcagttgat caatttccat acggatcgca gtttctggca
    13621 accacatttc aataaatgaa acagcatcat cgaatctctt acgaggtaac tcgccatatc
    13681 ttgggattga aaaggtacgt ttaaattcag accaaaattt tgagaatact tttttgctga
    13741 tttcttcata agctcggctt tcttttcccc ctagaactcc cataactttc atatttcctt
    13801 tttgcttaat ttcaaactct tgttgtccgc taattcgcat agtatcttta agcatggaaa
    13861 catctttttt aacatctttc atttcttcta attgatagat catcatatct tcaattgttt
    13921 gaggaacagt attcttgcga ataacatctt ccatttcgtt gaatgcttca atgtattttt
    13981 gtttgaagta gatagctttc tttcctgtaa accccatagc cagcaagaaa aatccatctc
    14041 tactaatgaa gaaaactcgt cgatttctgc catatgaatc tggttcatta ccttctacaa
    14101 acatctgtcc aaaattggac acatcttttt ttagtgcatc aatatctctt aaaacatgtt
    14161 gatgtttttt ctcgaagctt tctgccactt gtaagctcgt agtcacagct tctttatttt
    14221 tcaaaattac taattcttgc attatttctt ctctcctttt tggtataatt tagttaaaaa
    14281 ctggtggtgt ttattttgaa tttttttcac ttaacaattt ttcaatggat gtcaataata
    14341 agttttatat tgagtacgtt tttaacaggg ataaaaataa aaggaatgcg ccctcaacta
    14401 gaagttgaac ttaatgcttc ttacttcgct gcagatatga tttatacaaa agtaatcatt
    14461 tctaactatt caacagaacc agctatgtta gttaatttag aactttcatc cgctaactta
    14521 aaccgcagat ggtcagctac tccatttaag aaattaattg ctaaaggagg atcagtcgat
    14581 gataatagaa tatactctga agctgtaccg ctaaatattc ctcctaaaag tgctatatct
    14641 tgttaccttg catttgaagt agggaaaatt aattttaata aactccttaa tcaccaaaca
    14701 aaaatgatat ttactctaaa tagaacccaa atccacaaaa ttgttgatat caaaaataca
    14761 aacttcccaa tagagaaatt agtgaaagaa ttaaactgag taatagtaat atacattcta
    14821 gtttctcttt tctattcatt tttagtcagc cccctcggtt ggctttttcg ctctgtactc
    14881 agcttcagct tcatcaagcc ccataaaaat ccaaaccatg taaacaatcg ttcctatcaa
    14941 cgcttgcctg cttccccaaa gtcctaaagc gtagatgatt agcggtgcgc tgaacactag
    15001 cgctctatta aattttccca tccgcttgcc tccttaagat ttccgaaaaa tttgtttcta
    15061 aaaattcaag tgttttactt cttaaaaata gatatgtgtc tcttccttca actggataat
    15121 agacaaatcc atttttgttt ttctcgatat cgataatatt tctatatctt gggttcttta
    15181 aaactctaga agtaaaccaa tcatattttc tgttaatccg ttctagcact tctggcaacg
    15241 tcatccatct accagtatca tcagcttttt ttaactcctc gtaatccact tgagagataa
    15301 ttacatagcc ttctggaact gggattttcg cttctagata ttgcattagc tgttcctcct
    15361 attgaatacc taatattttt tttacagttt ctatgtgttc ttgagctttt ttaccatcac
    15421 gattgccgtt taggatatct gataaataag ctcctgaaat accaacaagc gcagctagtt
    15481 ctttgaaagt cattcttctt tttctcatct ccgctcgaat ttttaagtct aaattctcag
    15541 acataaaaat agctcctttc taaaaaataa tttgtaagct aaaaaattag ctaaatcgtt
    15601 gacacctatt agcttttaag ctattatgaa tacatagtta aataagactt ataaaaagcc
    15661 tctaaaataa catttctaag tttggcgacc gagagaatgt tttaaattag tagagttttt
    15721 tgttgctctt atttagctaa caatttagct tacgaattaa atatactagc ttaaaagcta
    15781 attgtcaacc aaaaatataa cttttaagct atttattttc ttttcagctt tgaaaggttg
    15841 ataataatga gtttagttac taagattaaa gaattagcag acgaaaagca tgtgactata
    15901 gcagaagtag aaagacaggt gggcatctct aatggacaaa taagaagatg ggataaagcc
    15961 tcgccaaaat ctgagaactt aaagaaagtt gctgattatt ttggtgtcac aactgattat
    16021 ttattgggaa ataataatgt tcccaaatgg gctacaaaag aggaagtggt tgaacttgat
    16081 aaactactag actcaaatgt taatatgtct tatggtgggg aaacattgac acccgaacaa
    16141 atacagcgcg taaaagatat cctgatagcg actttctggg atattgtgaa agaagacaaa
    16201 gaaaaaggca aaaagatgtg agcttatgga gatggatacg attaatttag tcgaggagtt
    16261 gaagcggaaa taccagtccg ctaatccttt ttatatttgt gaaaagatgg gcattaaaat
    16321 tcaatacgtt ccttttatcg agaatcccaa ggggcagttt caagaaatta gagatcgtgc
    16381 gataatcttt ttaaatgatg aactgcgaga ctctgaggaa agattctaca tttgcgctca
    16441 cgaattaggt cacgctattt ttcatcgtgg cttatccagc tattatgtat caacaagaac
    16501 atctagaagc aaatctgaaa gcgaagctaa ttgctttgct gctaatctca ttgtttctct
    16561 ttataaagaa gataatgatc aatatcctaa aaaaatcgag gaattaaaga atctttacgg
    16621 tcttccagaa agcgcttatc gttttcttat ataaaaaagc ccgtgttagc acacatatta
    16681 caacgagaaa gaggaattat aaaatgaaaa aagttagcgt tatgttgttg ttaagtactg
    16741 ctctgctact ttcagcttgt tcaaataata aaaaagctga atcaacagat gccacttcta
    16801 accaagaaac aaatataagt aaaacaaaag aaacaactga aatcagttca tctactagca
    16861 aatctacatc taaaacagat tctagttcaa cagttacaag ctccaaccaa gctacggcga
    16921 aacctagccc aacagttata agctccagtc aggatacaat ccaaaccgca cctcaagaag
    16981 aaacatatga acagatgaaa caacgcactt tacagtcaac tccagctgat cgtgcaaatt
    17041 ggtccaacaa agagtgggaa gctttcggcg tggcccttta tgaaaatgga ttgactacag
    17101 atgatgctgg caatattatc agtcaagatc agaaagaaca acaagctgca tctcaacaaa
    17161 atacagaagc ccaacaaaca agcgctcaac aagacgctga cactttatca cttactgatt
    17221 ttgttaacaa atacgggatg tcgcctgttg catggaaagt acagaatgga atgtctgaag
    17281 aagaagcatt gcgtacaaca cagcaaaaaa cttccggtga agttcaatta ggattttcta
    17341 aatacggaat acaataatat atttttgtgt cccactatct tgcctataac ctctaaaaag
    17401 ttataaagaa aaaagcccgt gctgcaacac ggactaaaat ctcatttcta agattctaca
    17461 taaaaataat atcatagaag tgaggaatta aaaatgaaaa aaaacttaat attaggattt
    17521 atttgtacta gtattttaac tttgggtgca tgtagttcgc cttcaaataa tgcaactaat
    17581 agtgaaactc aaaaaacaat caatagttta aaaaaagaaa atgaagagct taaaaaacaa
    17641 atagcatcgc agtcaaatga cactgaagaa aatagcgaat ctatcgacaa tgcttctaag
    17701 gaaaaaacct tgccagttgt tggtatccta gaatatacaa tttcttctat aaaaactgaa
    17761 caggtaacaa atgacgaaga taattttaca aatgccgaat ataactttag tggaatagaa
    17821 aattttccag aaaaatacta tcgtactact atttcttaca agttaaaaaa taaagcaaca
    17881 tcacctttct ctttgtcttc gtatcaggca tctttaatag atggagacgg tatggaatat
    17941 agtagaacag ctggagaata tttcttatat gacgaaaatt ctaattcttt agttcaacct
    18001 gaaacgacaa catctggggt tttctattta ctttctaaaa ataaaccaaa tttaagtaca
    18061 tttaaactta atgcgtctaa acaatttact gatgatttta cagctgagcc gattggcgaa
    18121 gcaggaacaa cagagtataa ataaaaaaca cgccccctcc ctcgccaaag tttgtggacg
    18181 cgttaaaaac aaacctacac aataggcttc tttacagtgc ctattgtatc agagaaagag
    18241 agtagattca attatggcaa gattagtcaa acgtggaaat agttggcaat acgaaatttc
    18301 atacaaaaaa gatgacggaa aatacacgaa gataagaaaa tcaggattca agacaaaagg
    18361 cgaagcaaaa gatgccgcca acgaattaga atataacctg aataaaggtc ttaaagggga
    18421 tcgcaaaaat ctattattat cagattactt tgaagattgg atgcaactct acaaagaagg
    18481 aacagtatct cctatcactt atagaaaata cgaagatacg ttaatgaata taaagaaata
    18541 tatgccagca gtattgattt ctgatttaga tagagttgga tatcaacgct ttttaaataa
    18601 gtatgcgaaa gaccatgtaa aatccaccgt tattaagttt aataaccata ttagagcatc
    18661 gttgaaagat gccgtagaag aaggattaat tccgtttgat ccaactagaa aagcagtaat
    18721 caaaggaaaa gattcattga agccaaaaga agataaatat ttagattatg atcaatttaa
    18781 atctttaatg aaactcgtag aagaaaacct ttctgcacag tactcttctc ctatgctcgt
    18841 gttagttgct ggtgctactg gaatgcgatt tgctgaactt ctaggattaa catgggaaga
    18901 tatcgatttc gaagatcaaa tcatcactat taataaaaca tggaattata aattaaatga
    18961 atggggaaaa acaaaaaacg agacttcaaa tcggaaaatt tcaattgata aacatacgat
    19021 tgatctctta aaaaagttta aaatcaatca aaaagaatta ttcgagaatt ttgaagttaa
    19081 aaaccctcat aattttgttt ttttcaactt aaaaaatgga ttagtttcat caaatgccgt
    19141 cagcaaatat ttgcgcaaaa aattaaaaga attagggatt gaaaagcaat ttactttgca
    19201 tggactaagg catac
//
LOCUS       DAZKNO010000042        19070 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000042 DAZKNO010000000
VERSION     DAZKNO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.042"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..211
                     /locus_tag="ACG8MY_001111"
     CDS             23..211
                     /locus_tag="ACG8MY_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0430326.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(269..622)
                     /locus_tag="ACG8MY_001112"
     CDS             complement(269..622)
                     /locus_tag="ACG8MY_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430327.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1153..1575
                     /locus_tag="ACG8MY_001113"
     CDS             1153..1575
                     /locus_tag="ACG8MY_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0430328.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            1572..1802
                     /locus_tag="ACG8MY_001114"
     CDS             1572..1802
                     /locus_tag="ACG8MY_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430329.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2300..2500
                     /locus_tag="ACG8MY_001115"
     CDS             2300..2500
                     /locus_tag="ACG8MY_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFQ0430330.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            2527..3720
                     /locus_tag="ACG8MY_001116"
     CDS             2527..3720
                     /locus_tag="ACG8MY_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0430331.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3788..5353)
                     /gene="guaA"
                     /locus_tag="ACG8MY_001117"
     CDS             complement(3788..5353)
                     /gene="guaA"
                     /locus_tag="ACG8MY_001117"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0430332.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5412..6335)
                     /gene="coaA"
                     /locus_tag="ACG8MY_001118"
     CDS             complement(5412..6335)
                     /gene="coaA"
                     /locus_tag="ACG8MY_001118"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0430333.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            6531..7136
                     /locus_tag="ACG8MY_001119"
     CDS             6531..7136
                     /locus_tag="ACG8MY_001119"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0430334.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7444..8460
                     /locus_tag="ACG8MY_001120"
     CDS             7444..8460
                     /locus_tag="ACG8MY_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0430335.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            8601..9902
                     /locus_tag="ACG8MY_001121"
     CDS             8601..9902
                     /locus_tag="ACG8MY_001121"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0430336.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9948..10610
                     /gene="deoC"
                     /locus_tag="ACG8MY_001122"
     CDS             9948..10610
                     /gene="deoC"
                     /locus_tag="ACG8MY_001122"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0430337.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            10631..11029
                     /locus_tag="ACG8MY_001123"
     CDS             10631..11029
                     /locus_tag="ACG8MY_001123"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0430338.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11095..12177
                     /locus_tag="ACG8MY_001124"
     CDS             11095..12177
                     /locus_tag="ACG8MY_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0430339.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12389..13951
                     /locus_tag="ACG8MY_001125"
     CDS             12389..13951
                     /locus_tag="ACG8MY_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430340.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13944..15077
                     /locus_tag="ACG8MY_001126"
     CDS             13944..15077
                     /locus_tag="ACG8MY_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0430341.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15077..16033
                     /locus_tag="ACG8MY_001127"
     CDS             15077..16033
                     /locus_tag="ACG8MY_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0430342.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16132..17298
                     /gene="deoB"
                     /locus_tag="ACG8MY_001128"
     CDS             16132..17298
                     /gene="deoB"
                     /locus_tag="ACG8MY_001128"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0430343.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17322..18140
                     /locus_tag="ACG8MY_001129"
     CDS             17322..18140
                     /locus_tag="ACG8MY_001129"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0430344.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18157..18864
                     /gene="deoD"
                     /locus_tag="ACG8MY_001130"
     CDS             18157..18864
                     /gene="deoD"
                     /locus_tag="ACG8MY_001130"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0430345.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6092 a         3453 c         4116 g         5409 t
ORIGIN      
        1 aagtggaaag gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg
       61 aaataaaaca agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg
      121 ccccaaatgt aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa
      181 agagccagac gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg
      241 ctctttttgt ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac
      301 cttttgctag tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt
      361 ctacctgtaa ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa
      421 tagatacatg gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt
      481 tgttttctat atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac
      541 gagtgagacc ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt
      601 atttatcttc ttttttacgc atactaaatc acctctatac attttactgt tcctatataa
      661 ccataaacag gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg
      721 aatttattaa tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat
      781 acaactttat aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga
      841 gggaaaagtc gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc
      901 aaaaaaactt atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac
      961 atagatagaa aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg
     1021 tcacttaata aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg
     1081 tcggggttta gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag
     1141 ggggtgaaaa gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat
     1201 atctgcaaac aagttattga agatgaacga aaagattact tcaaacatct agacagacta
     1261 tcagcaaaag aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact
     1321 gttgatacgt acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt
     1381 gaaagtgatt tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt
     1441 ttacttcatt actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat
     1501 cgttcgactg tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag
     1561 gagtgtaaag aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga
     1621 cagatggcga cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa
     1681 aacgttcatt acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa
     1741 ccctacgtgg cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat
     1801 agtcccattt tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt
     1861 tgttattcca tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac
     1921 gttctttgac aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa
     1981 ctaacacgcc atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc
     2041 tttagcagga ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata
     2101 gtccgagcgt tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt
     2161 gaaattcctg tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt
     2221 gtattattat aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat
     2281 aattgggaaa ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata
     2341 tggaacttct ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg
     2401 gcatatctac tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat
     2461 tctaggaatt cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta
     2521 aaaactatgg ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca
     2581 aatctaggtt atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact
     2641 aaaaaagaag caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt
     2701 gagaataagg ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat
     2761 aagacacagg taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc
     2821 tcttattttg ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa
     2881 ctaaaattat caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca
     2941 gttgctatgg atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt
     3001 ggcaatgtga aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa
     3061 gtaatctctc taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg
     3121 tttttattta tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt
     3181 gattttgact caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat
     3241 tatagatttg ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc
     3301 acattaactt atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt
     3361 gtaatgagct ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc
     3421 tatgctaagc tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct
     3481 tccttgttaa tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa
     3541 gatattgaga cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta
     3601 gctaacaaat taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca
     3661 ccaaagaatc aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa
     3721 aagtgcagta aaaagataag aagagccaga aacccttggt ataagaggat ttctagctct
     3781 cctacaacta ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac
     3841 ggttgacgtg ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg
     3901 ggatacgggc aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg
     3961 tgtagtcgta agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag
     4021 tgaaatattg ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag
     4081 catcagattc gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac
     4141 caagaccagg accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag
     4201 tccctaattc acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt
     4261 gcatatcttc tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg
     4321 taccagattc gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt
     4381 caccagcaag ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt
     4441 tacgtttttg ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat
     4501 cgactttgat gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt
     4561 cgcctttgcg tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt
     4621 tttgtaaaag aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt
     4681 ttttgtcacc aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg
     4741 tccagttacc ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt
     4801 attcggaatg acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt
     4861 ggatagaagc aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt
     4921 tagttaccaa gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca
     4981 agacagcttg atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag
     5041 gctctacgga accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata
     5101 caggaatacc taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac
     5161 tgtttggtcc gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag
     5221 taatgcggtg gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca
     5281 attggttata ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg
     5341 caacgtttgt caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact
     5401 attttaaaat gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta
     5461 tggagaatta tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa
     5521 ttcacatttt tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca
     5581 aaacgataat aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga
     5641 tccaaatacc atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa
     5701 aagtcgctga cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg
     5761 atcaagatat caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca
     5821 tgtgagtaaa caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg
     5881 atcagcatct ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct
     5941 tttaatatac gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt
     6001 ttgaaggtac gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct
     6061 acacttccgg cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa
     6121 cctttactca agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga
     6181 ggtacataga tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta
     6241 tctagttcat cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct
     6301 cttgccacac ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca
     6361 atggaactat tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc
     6421 tttttgtata atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat
     6481 acataaaagg atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc
     6541 attactattc tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa
     6601 aaggcagaac ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt
     6661 atggttcacg tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga
     6721 tcttagatgt gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc
     6781 ggtttgttga aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa
     6841 agcgtaacca aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg
     6901 aagaaaccta tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac
     6961 atgaaatttt gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga
     7021 tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg
     7081 cagaaatcgt cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag
     7141 ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca
     7201 gttctttttt tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat
     7261 agtaggaaca gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga
     7321 aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct
     7381 ctattctttt cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat
     7441 aggatgaagc tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc
     7501 tcacagattt tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg
     7561 tcgatcgtga atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga
     7621 cattcaaaga ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg
     7681 atcgaaggtg tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct
     7741 cgtcatagcc aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac
     7801 ggaatcttgc tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt
     7861 tatcaaaaaa tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg
     7921 cttatcgtaa aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc
     7981 catacgaaga tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc
     8041 aacggatatc gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa
     8101 catggagagt tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa
     8161 gatatcacgg ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc
     8221 gatgaattgg gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag
     8281 ctgtcagaat acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga
     8341 ttttatgctg cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac
     8401 aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa
     8461 ctcggcggta tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt
     8521 tagtaaacca gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg
     8581 aagacaagga ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa
     8641 acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc
     8701 cagattatca agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag
     8761 aaatcacgaa cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc
     8821 ttgaaggaat aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga
     8881 ttttagcccc cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc
     8941 taggttacac aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc
     9001 tttcggatga agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat
     9061 caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg
     9121 ttgattctct tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag
     9181 atgcaatcgt tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg
     9241 caagacgtct ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg
     9301 cggttatctc agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg
     9361 tcgaggcaat cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg
     9421 ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa
     9481 cgttgcttca agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc
     9541 aaaatcaagg gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct
     9601 acacaatgga gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat
     9661 taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc
     9721 acgctgttgg attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac
     9781 tattgacgat ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa
     9841 atattgaaat tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat
     9901 aaaggaaaaa agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc
     9961 gaatgatcga ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta
    10021 tcgaagaagc aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc
    10081 tagcgaaaga acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc
    10141 taggagcaaa cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg
    10201 cagatgaagt cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag
    10261 tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta
    10321 ttattgaaac atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag
    10381 ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag
    10441 aggatgtccg tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg
    10501 gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa
    10561 caagcgcagg agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa
    10621 ggagaagctc atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga
    10681 taaagcctat gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg
    10741 caagatttat caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga
    10801 gcgcacagct tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt
    10861 agcggggcac acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga
    10921 attctgttcc cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac
    10981 gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat
    11041 tcagtcagct gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa
    11101 aaagcaaaat tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca
    11161 tgcggcaaca acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact
    11221 gcagcattga tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg
    11281 gaaggactag aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa
    11341 tatttccaat cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct
    11401 ggattcaaaa cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca
    11461 acaaacaata caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac
    11521 gttgtatctg caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct
    11581 tatacgactg aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat
    11641 cgttttgatg ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa
    11701 atcaaagtat tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt
    11761 gctcaaggta tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt
    11821 aacggtgtat tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt
    11881 atcggtgttg accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa
    11941 aacttcacat tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa
    12001 aaatcagcgg acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac
    12061 ggtgttggtc taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca
    12121 aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac
    12181 catttctata gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa
    12241 ttgttcattt atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta
    12301 atgtttgttt caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag
    12361 atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca
    12421 gaatattacc aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa
    12481 ggcaggagaa attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa
    12541 cgtgctttcc ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt
    12601 aaagatcaca agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt
    12661 tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg
    12721 cgcagggatg cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta
    12781 tggattgtca gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg
    12841 ggtagaaatt ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac
    12901 agctgtattg acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa
    12961 agaaggcaag tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga
    13021 ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac
    13081 ctcacaacaa ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa
    13141 agaagcgaag cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg
    13201 tggattagaa gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat
    13261 cgctggaatc gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa
    13321 ggcagaaagc ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa
    13381 aatcactgaa catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct
    13441 agatatgaca ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa
    13501 aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata
    13561 tgatgttcga acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca
    13621 aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc
    13681 aactcgtgga ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag
    13741 ggacaaatac aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc
    13801 ggatcgtatt gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac
    13861 atctgaaaat gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga
    13921 gttagaaaag ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag
    13981 tgcctgtact ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg
    14041 gttttcagcc catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag
    14101 ggataggtga aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg
    14161 tagctaactc agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga
    14221 tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt
    14281 tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc
    14341 gagcattttt tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat
    14401 acgtaagcac atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact
    14461 cttccaatat gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg
    14521 gcggctctcg cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc
    14581 tgctgatgaa gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg
    14641 cttctgagta tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg
    14701 gcgctttggc agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg
    14761 ttcaaacgac ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg
    14821 gatcagcaat cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac
    14881 ttggcatgca gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga
    14941 ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag
    15001 ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg
    15061 aagggggcga actataatgt cgactatcga attaattgcc ttgatcctta cttctacact
    15121 ggtttattca acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg
    15181 gatcgtaaac gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt
    15241 caacttgacg atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg
    15301 tggcgtcgta ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc
    15361 tgaccatatt atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt
    15421 gatcaaagca tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt
    15481 ttctttccca ggattagcag atattccggt cattggacca attttcttta aaaatacatt
    15541 cttacctgct tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac
    15601 gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg
    15661 tatcaatgta tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat
    15721 gggcggtgct gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg
    15781 acaagggttc atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat
    15841 gggtgcctca ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc
    15901 agttatttct tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat
    15961 tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat
    16021 caaatcaaaa taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct
    16081 cgcccttttt gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa
    16141 cgcgtacatc tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag
    16201 ttcggtgatg ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt
    16261 ccacatttag agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg
    16321 aaagatcatc aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg
    16381 acaggccact gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat
    16441 ggattccctg atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt
    16501 aacaaacctt atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca
    16561 ggcgatttaa ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa
    16621 atcattcctt tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat
    16681 gatccgtata tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc
    16741 acacgaacaa gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat
    16801 tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac
    16861 ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg
    16921 ctgaaagtta tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat
    16981 gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac
    17041 ttacgaattc cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc
    17101 gatcacggaa acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta
    17161 ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat
    17221 atttcagcaa cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc
    17281 ttgaaattat taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga
    17341 tgttacaaga aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt
    17401 tgattctagg ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt
    17461 cttatgatga gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag
    17521 tttatgggtc tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg
    17581 aaggtcattc catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc
    17641 attcagtcat tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt
    17701 tgatgctgat taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg
    17761 aagatgcaat gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag
    17821 caacagcaaa agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg
    17881 gctattctgg accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag
    17941 ccgatgcagt agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa
    18001 aagtgttagg aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc
    18061 atgaagaagt tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag
    18121 aaacattgac attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa
    18181 gaaggcgaga tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt
    18241 gcggagactt ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt
    18301 accggcaaat ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca
    18361 gccaccatct atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta
    18421 ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt
    18481 gcagcgacca gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc
    18541 agtgatttca atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt
    18601 catgtaggaa atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc
    18661 caattagctg aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta
    18721 ggcgcgaaat accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc
    18781 ggtgaagaaa caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga
    18841 ttggaaacag cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata
    18901 agaacatcca agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa
    18961 atgtaagctt ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc
    19021 acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga
//
LOCUS       DAZKNO010000043        18374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000043 DAZKNO010000000
VERSION     DAZKNO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.043"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(114..1406)
                     /locus_tag="ACG8MY_001131"
     CDS             complement(114..1406)
                     /locus_tag="ACG8MY_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0430346.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1403..2026)
                     /locus_tag="ACG8MY_001132"
     CDS             complement(1403..2026)
                     /locus_tag="ACG8MY_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430347.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2029..2865)
                     /locus_tag="ACG8MY_001133"
     CDS             complement(2029..2865)
                     /locus_tag="ACG8MY_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0430348.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2882..3769)
                     /locus_tag="ACG8MY_001134"
     CDS             complement(2882..3769)
                     /locus_tag="ACG8MY_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0430349.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3785..5068)
                     /locus_tag="ACG8MY_001135"
     CDS             complement(3785..5068)
                     /locus_tag="ACG8MY_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0430350.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(5751..6434)
                     /locus_tag="ACG8MY_001136"
     CDS             complement(5751..6434)
                     /locus_tag="ACG8MY_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0430351.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6452..7069)
                     /locus_tag="ACG8MY_001137"
     CDS             complement(6452..7069)
                     /locus_tag="ACG8MY_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0430352.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7079..7903)
                     /locus_tag="ACG8MY_001138"
     CDS             complement(7079..7903)
                     /locus_tag="ACG8MY_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0430353.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7896..8456)
                     /locus_tag="ACG8MY_001139"
     CDS             complement(7896..8456)
                     /locus_tag="ACG8MY_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430354.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8630..9586
                     /locus_tag="ACG8MY_001140"
     CDS             8630..9586
                     /locus_tag="ACG8MY_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0430355.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9891..10847)
                     /locus_tag="ACG8MY_001141"
     CDS             complement(9891..10847)
                     /locus_tag="ACG8MY_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0430356.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            10951..11553
                     /locus_tag="ACG8MY_001142"
     CDS             10951..11553
                     /locus_tag="ACG8MY_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0430357.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11641..13140)
                     /locus_tag="ACG8MY_001143"
     CDS             complement(11641..13140)
                     /locus_tag="ACG8MY_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0430358.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13156..13680)
                     /locus_tag="ACG8MY_001144"
     CDS             complement(13156..13680)
                     /locus_tag="ACG8MY_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0430359.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13978..14727
                     /locus_tag="ACG8MY_001145"
     CDS             13978..14727
                     /locus_tag="ACG8MY_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0430360.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            14718..15503
                     /locus_tag="ACG8MY_001146"
     CDS             14718..15503
                     /locus_tag="ACG8MY_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0430361.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            15472..16188
                     /locus_tag="ACG8MY_001147"
     CDS             15472..16188
                     /locus_tag="ACG8MY_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0430362.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(16290..17309)
                     /locus_tag="ACG8MY_001148"
     CDS             complement(16290..17309)
                     /locus_tag="ACG8MY_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0430363.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(17328..>18374)
                     /locus_tag="ACG8MY_001149"
     CDS             complement(17328..>18374)
                     /locus_tag="ACG8MY_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0430364.1"
                     /translation="DIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVGKILS
                     KVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQ
                     TTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEA
                     KNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQP
                     MDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTA
                     TNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVW
                     IVVLQMIHGIGLFS"
BASE COUNT         5493 a         3884 c         3238 g         5759 t
ORIGIN      
        1 acaaaaagca tgtcatagcc cctttttccg aataaatagt gttcaaaaga cagcctatcg
       61 gcaataaggc aagattttat aaactacggg aagtaatttc cgtaaatcta ttcttattct
      121 tttaagctga caagctcgtc aatcactact gcttttcctg aagcaataga atgatttgct
      181 gcgattcctg tcaaaatcga caaaatacca tctacgtgag ttgctgcccg attccacgga
      241 tcttcttctt tttcttcacc aaatagatca tctaataaac gcggatctcc tccgccatgt
      301 ccgcccgctt cttgttcgac gattacctca tatggctcac cgaacatcgg atagacatgg
      361 atggactgaa gaacggctgc tccttcatcc tttttatctc cacctgcatt gatgtaagac
      421 ttttcgatca ctttcatttc gatccgtcct ttgctgccat tgaaagaaac attgaatcct
      481 tcccacggca tatacgcgtt tagcgaatac gtcaaaatcg caccagtttc atatttgacc
      541 gttacgccta gtgtatcttc aatagagata tcatcggaaa atacactgcg gtcacgaata
      601 tatccactgt ctttttctgc ttctaaatac atttctcgca attcctcatt ggctgacagg
      661 tctatagcaa atggatcagc tttagctagt tcgcttccat agcatcgact gtaaaatatt
      721 tctactccgc gtttctccgc attatgcatc ccataaaagt ttaatccacc gaatgcaaat
      781 accttttggg gacggctgtt caaccaaaaa ttcactagat caaaatgatg ggtagattta
      841 tgaacaagaa gcccgccgct attgtgctta tttcgatgcc atcttctgaa ataatctgcc
      901 ccgtgttgcg tatttaacag ccattcgaag tggatagaaa acacatcacc aatcacatca
      961 ctttctatca gttcctttat cttcgtgtga tacggtgcat aacgataatt aaaagtgact
     1021 cgcaggcttc ttcccgtctg tttttggacc gttattattt cttgagcttt ttcctcatcg
     1081 atagtcagag gtttttcgca aataacatca tatccttttt tcattgcacg aatgatataa
     1141 tcatgatgcg ttcgatctac tgacgtaata atgacttcag ccggccgtac gtcatccagt
     1201 agttgctcaa attgtgatgg catacatgtc tttacttttt tctgtccata cttgttcaca
     1261 agtatccgat ttgcataatc catccttgtt tgagatacat cacaaaaacc gactagttca
     1321 caagtttgat gatacgtaga ggcaattgcc tcataaaaaa aacgcgctct cccaccaagt
     1381 ccgacttgta catatttttt cttcatttaa agtcattctc cttttctttt aagcaaaata
     1441 ctgtgacaaa tggcagccct ggatacagta atgcagtgag taaaaaatca tgctgtatac
     1501 accagaacag tccatacaaa aggcataaat accacagaaa ccccttgacg ttttttgaca
     1561 acagccagat attccttttg atcaaataaa caagactatc tgctttatct tctttgcaga
     1621 tatcaattag cgtactaaaa aaactatggg atacaaaaag caacagacac aatagaaaag
     1681 gaatcagata ccaagctcca atctttccca ctaataaata ccaatcaact gcaaaaataa
     1741 tgacaaacag ctgaaacaac gtaatgatga aacacatccg tttgattttt cctttcctcc
     1801 aacatacctt tttttctgcc aagtaatgga cagatagata ataagaagga tacatcatca
     1861 aaaaataaat cagatagaca acacgagcag acagcactaa tcctaaagtg aacaaacatc
     1921 ctagaactaa agcgaaaaga gcattactga taagactggt taaaaaaatc cgacagacat
     1981 agtagttgag cttgtaaaga aacatctctt ctattggctt tttcatattc agcccttcag
     2041 accgcttgta ctgatcccct ggacaagata tttattgaag aataagaaaa tcagaaaaac
     2101 cggtagcagc gacaacgtag acatcgcaaa catactcgcg taagtcgtac tagatccagc
     2161 gtctgcaaat aatttgatgg caatcgatac cgtgtaagat ctcggcttgt ttaaataaat
     2221 caatggtccc atgaagtcgt cccatttcca ataaaactgg atgatgatcg tcgtgatgat
     2281 cgaaggtttc agcaaaggaa aaataatttt ggtaaagaca ctatacttgc tgcatccgtc
     2341 aataatcgct gattcatcta actctttggg gatgctgacc atgaattgca tgatctggta
     2401 aatgaaaaat gcttgtccaa aaaaatgtgg taaaatcagc ggcacatacg tcccaaccaa
     2461 attcagccga ttgtaaataa taaactgcgg aatcaaaatc acttgtgcag gaatcatcat
     2521 cgttactaac atgacgacga accagaatct ttttcctttg aaatcgattc tcgataatgc
     2581 gtaagcaata caagtcgagc tcagaactgt ccctatcgtc gaaactgcag agatgacaaa
     2641 cgaattccta aagaaagtaa aaaatgaaat tccagcaaac cccgcccatc cttttgcgta
     2701 attttcccat tgaaaagtct ctggaatcaa attcagcgaa ccactcataa tctcttgatc
     2761 cgttttaaaa gaacctgcga tcatccataa gaccggataa atcatgacta gacctaatag
     2821 caaaataaaa ccgtattgaa accatttcgt tactgtatta ttcatttctt cctcctctca
     2881 ttcacacaac gacccatttt tttgatgatt tgaaaatcag aaaggtgaca gctcccataa
     2941 tcaacagcat gatccacgaa atagcactag cataccccat agaattaaaa gaaaaagctt
     3001 ggttatacac ataaagagca taaaagttcg tggcatcatt cggaccacct tgggtaatga
     3061 taaatgcctg cgtaaaagtc ataaacgccg cgattgtctg cataaccaag ttatacaaaa
     3121 tgatcggcga aagaagaggt aacgtgatct tgtaaaacgt ctgccatttg ttcgctccat
     3181 cgatctctgc cgcttcataa tacgagcctg ggatctcttt gatacctgca gcaaagatga
     3241 tcattgccga accgaattgc caagcagaca tcaaaacaag agggatcatg accagttttt
     3301 gatcgctaaa ccaggaaagc gcaggtgcgc ctaatcgttc caggaaaata ttcactaacc
     3361 cgtttttaga aaacaactcc cgccaaacta gagcgataga aatactgcca ccgatcaaac
     3421 taggcacata atacaaagaa cgaaagaaag tcgttccttt gatattcctc gttaaaagat
     3481 aagcaatcaa tagaccaaag atcagtttca caggtacaga tgcaaataca tagagaaaag
     3541 tgactcggat ggagttcaaa aaacgggcat cactcagcat ctttatataa ttatccaagc
     3601 cgatccatga gactggattt cccaaacgat aatcggtgaa cgaataataa agtgacaaga
     3661 tcatcggtat cactgttatc aatagaaatc ctaggatgaa tggagaagca aatacgtaac
     3721 cgaccacatt gtcttgatcc aagaaccgtt ttagtttttc ttttttcatg ccttcctcct
     3781 gctgctatcc tagaatgttt gacgcaaact cataaagact ctctgctgct tcttctgctg
     3841 ttacttcttc ataaatgact ttttcaacta aaagattgta ttggtcttga atttccggat
     3901 tcaaagcact atcaaagaca ttcacttcgc cgttgtccat atcggaaatc atttctaaat
     3961 aatcaaatgt cgcttgcaaa gttgcatcat ctgcacgagc gagctgttct cgaatattcg
     4021 acatgattgg tacaccacgt tctcctttta aaatttcatt ggcttcttca ctgttttgaa
     4081 agaaatcaat aaacttagca gcttcttctt tatgggctga gctttttgcc atactcagca
     4141 tttgcgaaga acgtaaaggg ataccagatt ctccatctgc ttttctgcga ggtggattaa
     4201 tcaactgaat ctccatatct tcaggagccc ctgacaatat ttctgtcaac tggttacttg
     4261 atagaaacac cattgccgct tctctatccc taaccgcctg aaaagacaga tccgtcgtac
     4321 ttgcagctcc accacctggt tctggatggg cccctttgtc taccagattt ttacgcagct
     4381 ggaaaaaatc tgttagatag tctggatttt caaatcccaa gctgttagat agatcttccc
     4441 gattataaaa attcaatcct ttctcaactt gtggtatttg aataatacag ccagctataa
     4501 aatcttcgaa tcttgaaata gcatagattc ctaattcttc agtgatttga tagctgattt
     4561 gttcaaattc atcccatgtc caatttgaag ctggttcagg gatatttaat tgttcaaata
     4621 tatttttatt gtaagcaatc gcaaacgaat tcaccccgtt agacagtcca actaattgcc
     4681 cgtcgtaagt cgtcgttgct aaataggatt catctgcatt agaaatatca attatccctt
     4741 gctccacata ttcatctaat ggttcaatct gattgatgta ttgaggaaat tgattgttca
     4801 gttggaaaat gtcccagata tcatctgatg ctgctagtgt acctaatttt gtgtaatatc
     4861 cttcataatc ataaaattca taatcgatcg tcacatgggg atttttttct tcataaagct
     4921 cgattacttt tactgttgca tcgtgtcgga cttgcgaacc ccaccaagcc atgttcagcg
     4981 tgattttttt gtcatcttct tgcccacaag ccgtcaagaa gaataacata acacttaaag
     5041 ctaagatgat tctcattctt ttcttcattc tctgcacctc tacttccatt gttatttttc
     5101 aatatactgt atttttatac agaacatatt tattaaacga tctttttcaa ggggaataaa
     5161 aaccaagact tgccttatcg ttctgcctag gatgaacaac tcaatacttt caagaaaaat
     5221 tagatagtgc tgattcttct ttcatcgcta aaaacacttc ttgcttcaat gatgggagga
     5281 taattacgca aagaaaaaag gatatcagac aactgcccaa tatccttgac atagctaaat
     5341 aagcatgctt cttcgtgtgc gctcttccct aaaaaacaga aaaataacta gtaattctgt
     5401 atgaaattag gtacaatata cacgtcgatg cacaagcatt gactatggca acttgattgg
     5461 tcagttcccg ctgacttttc aagccccgac aatttagctg ctactaaatt gtcgggtgcc
     5521 ttttttagtt tttattcgtt tttcattcta tagaaagcgc ttcaataaat caagacattt
     5581 ttttgttaga atgctaataa taacaaaagg ttttaactct tcttatgtac attacaggta
     5641 aaaaaacaaa aaaatttctc ttttttttga attctgaaca aatagtactg gagtacaaaa
     5701 gacgaggaag agccactatc tgctcttcct cgtcttttga ttctcacctg ttattcatga
     5761 ttctttttct cagtttcccg atatttttcc attttacgac taaaaagctc tccgctactt
     5821 ggtggcgtat atgtgatggc atttgctcct gcttgaatgg tctcagcaat actttcttcc
     5881 gttggaccac ctgtcgcaat gataggaagt tcaggatatt ttttacgaaa atggcgtact
     5941 gcttctgcag tttctttacc tgcactgata ttgataatcg aaataccagc tgctaacttt
     6001 tcatctattt ctgtatattt cgacgttacc gtactgataa ctggaatatc aactactcga
     6061 caaacttctt ttactgtctc taaaggcgta ggaccgttca aaacgacccc taacgaacct
     6121 tgcgcttctg cgaacatgct catgtaggaa gaacgagcac cttgtgtcaa accaccacct
     6181 acaccggaga aaactggaat gtcagctgcc tcaataatgc ttttagtaat cgctggatga
     6241 ggtgtaaaag gatacacagc aattactgca ttcgcatttg tattacgtat gatcgcaata
     6301 tccgtagtaa agatgatcga acggatcaat tttccaaata tcttgatccc gcttgcttct
     6361 aaaatgattt ccggtactct tacgatatct tttctcaatt ccgtcatgat ttctggcgta
     6421 aattgttctg ccatcagcca cttctccttt gctatctccg gtcatactga ttcaattgat
     6481 acgtctcgat gacctggatg attttttgcg cataaccggg gtctgtcgca taacctgcat
     6541 cttgcaatgc ttgtgcggct tcctgatagt tggtggccgt aatcactttt tcatacagcg
     6601 caggattcca atctactcct tggacgaaca gttgtgtatg gtcatccatg gactgttccc
     6661 atgaatcata cactttgaag tctccttgga tagttatcca ttcttcgtta acaaattcct
     6721 ttgtttccag gcttactttc ttctgatccc cataagcctt gatcccgaaa agattattgt
     6781 atttgctagc caaggtactt tttccccaat ttgattctaa tatagcttgt cctaaaatga
     6841 tactaggaag cactccataa ccatcttgta gttcttgggc atgcggaaca agccgttcaa
     6901 taaattgctg atgggattgc gcttcatttt cttgctgatt atcagtaaaa ggagaagaca
     6961 gattcttcaa ggaaaaaaca aaggctaaga gtatcactgc tacaccggca accactgctg
     7021 gccaacggat attcttgcgc ttttgctttt tccacttttt tttcgccatg atcaattcct
     7081 acttttcatt taattcattc agatattctt ctagcgtcaa ttggtgtttg gtgatatact
     7141 cagcgttttc tttaccgaca taacgtaagt gccaaggttc atatgtgatc ttagttatat
     7201 cttcgtgtcc ttttggataa cgaacgataa atccatactg cgcagcatta tctgcgatcc
     7261 atttagctcc aggctgatct ccgtaacttg catccaatac agtagaagga taactgttgt
     7321 accagttttg atcgacgaca tcgactgcca gacctgtgtg gtgttcgctg tatcctggct
     7381 ccgtaatcgt ttctttagta atctttgtcg cctcctcttc tgatacacct tgacgactca
     7441 tcacatcttg gacattttgt tcgaaaactt gttgttggga tgatacagac cggtaagctg
     7501 agaccatcac taatggaaat ccagctgctt cagcagcctt ggcaaatgct tcatattctg
     7561 gagcaatgcg tttgtcgatc aagtatccgt tacttaatga tgccaattga gtagactcat
     7621 caatttcttt ttctaatttg tgatctggac caaccaaaac tagcttccaa tcgtctgttt
     7681 taacatctgg caattgtttg gacgtacgat cttttgtttc tttgtccttt accttgctgc
     7741 tggttgtttg actgttcttt tctttactag cagttgtttt ggtgcttgtt cgttcgactg
     7801 cttgattgcc ggcttgaaca tgcgaatcat catcgctaaa aataacatag cccattgctg
     7861 cgatcataat cacaactgct ccaaatccta aaaatttatt cacttgaaaa atcctttcgt
     7921 tgttcttcac gttccccatt cgcctcatga acgtaagttt cttccagatt ttcattcacc
     7981 caatcgagaa gttcgatttt ttctccttcg atctgttttt gaacatctgc tggcagacga
     8041 aaattcaaaa tatcatcata gccccattca tcatattgaa tacttacttt caagtcaagg
     8101 tatctaccag cctgctgatt atttttcaaa gggatcagct cgacttttgc tgctttgctg
     8161 ctaagccatt tttgcgcgat gcgtgttttg atttgcttgt actctgcaac ggcaacgttt
     8221 cgtttttcgg gaaaaataat cgtttgacgc aaaatctttt gcgttagcat ccattcataa
     8281 tcagtgaaaa gattttttac tttctgcatc gcttcagatg gcagattttt ctcgccatct
     8341 ttccattttt gccattgttc tggactgatc agcaagtatt catctaaaaa atttttttca
     8401 gtttcaaact gttctaataa tacactcata ataagatcaa tatatacttg atgcacgttt
     8461 atcccctcct tttaactatt ctacttaagt tttgatcaaa aagcacctct taaatcgctg
     8521 ctttcatttt ttttaatgtt tcaagtcctc tcctctttat tcttcgtgat ttttgttata
     8581 ataatagaca gacaagaaac tcaatccaac tatgaaggag cgttttcaga tgaaaaaata
     8641 tcaatgggga atcgttggtc ttggtaccat cgcacatgag tttgccgaaa gcttcaatca
     8701 agagacgagc gaactaacag cagttgcttc cagaacttca gaaaaagcgg aaaacttcgc
     8761 tcaccgttac aatattccaa aagcttacgg aagctatcaa gaaatgttag acgatgctga
     8821 aatcgacatc gtgtacattg ctgtaccaaa tagacagcat atcgaccata tcttggcagc
     8881 attggaagca ggcaaacacg ttctatgtga aaaagccatc acgatgaata aaaaagaact
     8941 ggctgacgct atgcgcttag cagaagaaaa gaatttgatc ttagcagaag cgatgacaat
     9001 cttcaacatg ccactttatc agcagttacg ctcaataatg gatacaggaa aactgggggc
     9061 tttaaaaatg atccaagcac ctttcggcag ttacaaagaa ccagacccta aaaaccggtt
     9121 cttcaatcca gaattagctg gaggagctct gttggatatc ggtacgtatg ctgtatcttt
     9181 cgctcgtttc tttttgagct ctcaacctga agtagttgcc tctacaatgg ttccttttga
     9241 aacaggcgtg gacgaacaat ccgtcactat cttgcgtaac aaagaaaatg aacttgctgc
     9301 tgtcagcttg acttttcagg caaaaatgcc aaaagtcggt atcgttgcct ttgagaacgg
     9361 ttatgtgacg attgctgatt acccgcgtgc tgatcgtgcg gagatcttat ttacagatgg
     9421 tacaaaggaa ttcatcgaaa gcgggaatac atctgaagca atgaattacg aaataaagaa
     9481 tatggtacac atgattgaag gtgacttgcc caatcgctct ctcttcttga caaaagatgt
     9541 catcgaaatc ttggatcaaa tgcaagttct atggaaaaat atgtaatttt aaaaggttgt
     9601 gaaaaagctg ttctgcatca agtaataaga acagccaaac aaaaatagct cctcctattt
     9661 ttcgtttggc tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg
     9721 ttgtgagcct accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat
     9781 gtttgttgaa tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag
     9841 aggttgtgaa atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg
     9901 attttttgtt gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac
     9961 tggaacaatt ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga
    10021 tacaatacat aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa
    10081 aatgtttctg cagtataaat caataaacca tagatatgtt ttccataata agctggttgc
    10141 tggattctct ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata
    10201 gagaagtaag tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg
    10261 gcatcttctt ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct
    10321 tctttatttt ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt
    10381 tcgatctgta tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca
    10441 agcaaagcaa atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa
    10501 gacatgtttt cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg
    10561 gttaatttga agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa
    10621 atagtatacc caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct
    10681 gcgattcgat tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat
    10741 gcaatgatcc ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa
    10801 aagaagacac tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt
    10861 ttttatttta attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg
    10921 atagtaaaaa gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg
    10981 cgtacgatca aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa
    11041 cgaggaatcc cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact
    11101 acaaccgttt cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct
    11161 atcatttatt tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg
    11221 cttcctattc tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt
    11281 atcatttccg caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc
    11341 gctttgtatg ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt
    11401 atcaatttct ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg
    11461 gtcgaactcc aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag
    11521 atccaaaaac aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat
    11581 ggagagaaga actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact
    11641 ttattcttcc ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg
    11701 gctagtagtc ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt
    11761 cgcttttagt gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt
    11821 cgctccactg gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt
    11881 tcctagcggc tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa
    11941 gacagctgct tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc
    12001 tgcttttcct tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt
    12061 ggcttccgct tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc
    12121 tttgactttt tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt
    12181 cttcacttcc gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat
    12241 ttgtttttgg cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc
    12301 tcgggcactt tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg
    12361 tttgtaggtt gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg
    12421 ctgtaattca gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt
    12481 tagagcttcc gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc
    12541 taaagagtca agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat
    12601 caaccccatt tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc
    12661 gcgattttga tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc
    12721 taatacttct cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc
    12781 tgctgtagca atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat
    12841 caccggtacg ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa
    12901 tagactgatc cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac
    12961 aattttgatt ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc
    13021 actgatgatc aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag
    13081 caacatcagt aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat
    13141 ttaacacacg ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct
    13201 ttccttgatc tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct
    13261 gtaaaaacaa gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat
    13321 acctcgtaca gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga
    13381 agacgacatc gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa
    13441 taccaacact atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt
    13501 taaacgctca cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat
    13561 cggatcaagc gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa
    13621 tcctgcacaa atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat
    13681 atacacgcct ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct
    13741 tcttttaccg actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt
    13801 taaatttttg ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt
    13861 ccttttttta agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc
    13921 tccacattcg tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg
    13981 aaccgatcta gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa
    14041 ctcatccaaa aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat
    14101 gaaatcatga ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga
    14161 tattctcacg cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa
    14221 aaagtgattg gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc
    14281 cgctatttcg tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta
    14341 acaaacaaca cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg
    14401 attattggac ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa
    14461 gtactgcttg gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc
    14521 gaaaatgaag tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc
    14581 aatacacctg caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga
    14641 aaaaaatcag tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga
    14701 ccgttcggac tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc
    14761 tttttagcaa tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa
    14821 tgaaattaat ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa
    14881 cccatgaatt catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg
    14941 aagaagtcac ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag
    15001 tctcaagtct gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag
    15061 ccaaagaaca atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga
    15121 tggatgactc catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag
    15181 aagccgtgct acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca
    15241 cagaagctga agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa
    15301 tctcagaaaa aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat
    15361 tgaatctcat ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa
    15421 caaatggcgg tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca
    15481 aatgtcctga taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa
    15541 agcatcccta atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat
    15601 ttcagacaaa aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt
    15661 atccattttt tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact
    15721 gctgcagatg atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg
    15781 atcaaaccgt tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct
    15841 cagacaatcc aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac
    15901 accactccca ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg
    15961 actttaaaaa aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa
    16021 ctggctcaaa aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta
    16081 gaacaagata tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt
    16141 tatgaattga caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta
    16201 tcccatacaa aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa
    16261 acattttatt gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat
    16321 acctcatttt tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa
    16381 atcatatttg ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta
    16441 gggtacattt ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag
    16501 gatcggaaaa aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga
    16561 ttgcttttca aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta
    16621 atcaagatgc tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt
    16681 tcacctggga tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca
    16741 agcgagtgga acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc
    16801 actttggctc ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct
    16861 atgatcccta ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa
    16921 tctttgaaag atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa
    16981 gtcaaagata actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata
    17041 ccaaaatcag ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca
    17101 taacgaatcc catattcctg catttttgca agattggtct catctgctat acaatttccc
    17161 ctcaacaata cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct
    17221 tcataaagac tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc
    17281 ggacgaacac cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa
    17341 cctatgccat gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa
    17401 atagttgaca gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca
    17461 taggctgctg ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct
    17521 tcaaaattga ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat
    17581 gcaggtacaa ataccacttt gactagactg taataccatg aagttttttc agatgcagct
    17641 tttttgaagc ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt
    17701 aaaccagcta aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa
    17761 aacgctactt ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct
    17821 tggaacagcc aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc
    17881 gctttgatat ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac
    17941 aaaaagattc ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa
    18001 atcgcactga cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc
    18061 aacgttgttt gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga
    18121 ataatataaa taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta
    18181 ttgatatctt gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact
    18241 ttcgatagaa tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct
    18301 aataatataa taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc
    18361 gatcgaaaaa tgtc
//
LOCUS       DAZKNO010000044        18214 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000044 DAZKNO010000000
VERSION     DAZKNO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.044"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..1190)
                     /gene="mnmA"
                     /locus_tag="ACG8MY_001150"
     CDS             complement(69..1190)
                     /gene="mnmA"
                     /locus_tag="ACG8MY_001150"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0430365.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNHAEVVFD
                     EPVRAITPGQAVVFYDEMECLGGGLIDHAYQETKVLQYV"
     gene            1594..2193
                     /locus_tag="ACG8MY_001151"
     CDS             1594..2193
                     /locus_tag="ACG8MY_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0430366.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(2346..3667)
                     /gene="efmA"
                     /locus_tag="ACG8MY_001152"
                     /pseudo
     CDS             complement(2346..3667)
                     /gene="efmA"
                     /locus_tag="ACG8MY_001152"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
     gene            complement(3997..7650)
                     /gene="rpoC"
                     /locus_tag="ACG8MY_001153"
     CDS             complement(3997..7650)
                     /gene="rpoC"
                     /locus_tag="ACG8MY_001153"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0430367.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(7729..11346)
                     /gene="rpoB"
                     /locus_tag="ACG8MY_001154"
     CDS             complement(7729..11346)
                     /gene="rpoB"
                     /locus_tag="ACG8MY_001154"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0430368.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            12074..13042
                     /locus_tag="ACG8MY_001155"
     CDS             12074..13042
                     /locus_tag="ACG8MY_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0430369.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            13060..13965
                     /locus_tag="ACG8MY_001156"
     CDS             13060..13965
                     /locus_tag="ACG8MY_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0430370.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            13962..14768
                     /locus_tag="ACG8MY_001157"
     CDS             13962..14768
                     /locus_tag="ACG8MY_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430371.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            14925..15893
                     /locus_tag="ACG8MY_001158"
     CDS             14925..15893
                     /locus_tag="ACG8MY_001158"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0430372.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(16210..16734)
                     /locus_tag="ACG8MY_001159"
     CDS             complement(16210..16734)
                     /locus_tag="ACG8MY_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0430373.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            17021..>18214
                     /gene="scm"
                     /locus_tag="ACG8MY_001160"
     CDS             17021..>18214
                     /gene="scm"
                     /locus_tag="ACG8MY_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0430374.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDS"
BASE COUNT         5264 a         3951 c         3481 g         5518 t
ORIGIN      
        1 gctatgctat ccaaaagaaa aggtgtttct tttgttagcc atagctgttt tgattcattt
       61 aatatcgctt aaacgtattg taaaactttt gtttcttgat atgcgtggtc gatcaaacca
      121 ccacccagac attccatttc atcataaaat acaactgctt gtccaggagt gattgcacgt
      181 acaggttcgt cgaatacgac ttctgcatga ttatcatcca ataagcgaac tgtcacagga
      241 acgtcttgct gacgataacg gaatttcgct gtacacttga attcttttgg cattggttca
      301 ttcgtcgtaa agtggatttc acttgcatcc aagcttgtgg catacaatgc aggatgatgg
      361 aacccttgcc ccacatataa tgtgtttgtc gctagatctt ttcctacaac aaaccagggt
      421 tcttgcgaat ctcctccgcc accgatccct agtccttgac gttgaccaat cgtataatac
      481 atcaagccag catgttgccc tttgacttca ccatcaagag tgaccatatt tccttttttt
      541 gctggcagat agttgctcaa aaattgtttg aagttctttt caccaataaa acagattccc
      601 gttgaatctt ttttcttagc agtcgcaagg cctgcgcgtt cagcaattgc ccgtacttct
      661 gatttttcca tgccgcctag agggaacatc gtttttgaaa gctgttcttg tgataattgg
      721 ctaaggaagt aagtttgatc tttattattg tctactccac gaagcatgtg gaccacgccg
      781 ttttcatcgc gagtgacttg cgcataatgt ccagtagcta cataatcggc gccaagatcc
      841 attgcataat ccaagaatgc tttgaatttg atttctttgt tgcacatcac gtctgggtta
      901 ggtgtacgtc ctgcgcggta ttcggctagg aaatattcaa atacacgatc ccaatattct
      961 ttttcaaaat tcacagaata ataaggaatc ccgatttgag cagctacttt cgctacatcc
     1021 ttataatctt ccgtggccgt gcatacaccg ttttcgtccg tgtcatccca atttttcatg
     1081 aatattccga tcacgtcgta tccttgttcc tttaacaaaa gtgctgttac agatgagtcg
     1141 acaccgccgc tcatccctac gacaacgcgt gttttgctgt tgtctgtcat gatatcacca
     1201 tcatttcaat tagattctga aacatctacg agttgaaggt cgcaactttt cagtatttta
     1261 cattatacgt catatttctg aaaatttcat cccttaatta tcaagtcgta aaaatactgt
     1321 tctactggac ctattcagta cacaaacatg atagtcaacc aaaaaatcac tcatcttttg
     1381 agcattcgat ataaaaaatt ggcatactca taaaaagggc cagcctttcg gcaataagga
     1441 ataattttat aaattgtgaa aaactatttt tcaaaattgc ttcttatcgc ttaaagctac
     1501 acgtctcatt tacaaccttt tctttttaga tgttatgagt tgctcagata agtgattttg
     1561 tttactctaa gaaaaaaggg aggcaaaaga tacatgaatt actttaccag tgatatgcac
     1621 tttttccatg aacgcttatt aggaaaaaac gactttgctc cgcggctctt tgcaaatgtg
     1681 gatgaaatga accaacagtt gatcactagc tggaatgcag taataaaaga aaccgaccat
     1741 gtttatcatc taggcgacct tgctatgcat ccgcaatatg aaaaagcaaa tgcagaaatt
     1801 ttggaactgg tcactcaact gcaaggaaca atccacttga tcaaagggaa ccatgacagt
     1861 cgcgcttttt tccgttattt agaaaaaaat gatcctaggc ttactgacgg aagtcccaaa
     1921 ttttattttt atgatgtagg tgccatcgtt aaattcgacc atcaacaata ttacttgact
     1981 cattatccga tgttgctcgg gcctaacgac actatccgca atcttcatgg acatatccat
     2041 cattacagcg tacctatcga gaacgatgtc aatgtaggcg tagacgcacc ggaaagggaa
     2101 ctcttaaagg aaaaactacc atttggcact ccgttatctg agcaacaaat caaagaaatc
     2161 tatgaagcaa agaggaagta cgataggtcg tgaaaaaact gccctatatc aagtattaaa
     2221 caaataaaga taattactca tatttttcat ttgagtaggc ttattgccac aggaacatta
     2281 ggatcagaaa tggagttgta acaagacttc tgtcacaact ccattccata ctattcgtct
     2341 ttttactaat ggtgttcttt taccaatttt ttctgtaatt ctttatcata atttcgagca
     2401 gctgacgtta aaaataaaac gattccgcat aataaaatcc cgattccaga aaacaagaac
     2461 attttttcta cccctaaacg atcagctaat ggtcctgcaa ataataatcc aattggacct
     2521 gccaggttca tcaaagagtt gaagttccct aagacacgac ctaaaacatt cgattcataa
     2581 ctctgctgga tcattgccat cagcagtgta ttgaaatatg gcgttgcaca acctgctcct
     2641 gcattcaaga taagaaagta caagaaccct tgactatcgt ttggacccaa tccgcttgca
     2701 ccgatcgtca ggcccataat gatgtaggct ataataatag gtttcattcg gtctttccat
     2761 gttcctataa aactgatcag tgcaccgcct aagagcattc cgactgcgta gactacttcc
     2821 actgctcccg cctgtccaac ggaaccacca aagtaaccta gcgtcatcaa tggatacaga
     2881 ctgattgctg gcatgaacaa cagcatcaca aatgcaccat tgatcgttat gtaccataac
     2941 cctttgttat ccatcaattg ctgcagccca aattttgcat ctttaaaaat agtaagcgga
     3001 acttccaata tttcaggaga aacttttggg atcttcacaa aaatcaataa gccgacacca
     3061 aaaactgccc ccaatacatc taataaaata aggtaattga caggaacgac tgaaaacaaa
     3121 gccgcaccta gagctggcgc aatgatgaaa ttcgctgatt ggaccattcc taactgtcca
     3181 ttcgtccttg ttatgtgaga agatggaacg attgtcggca taatcgactg atcgttggca
     3241 tttggaatgt ttgtgcgacc gaacgcataa acaaagacac aaaaactagc caaatcggga
     3301 aggattctga aatcgttccg actactgcta aaataagcgc gaaaatcgct acaataatat
     3361 ccgtgacaat caaaagagct tttttatccc agcgatccac tagcggttct acaaatgggc
     3421 ttagtaaaac catgggaatc attcctagta aagtagcaaa actcaaaatt gtcgccgaac
     3481 cggtttctct tgtcaaatac cagatgatcg catattggac gaccatacta gtgattcctg
     3541 acaaaaactg tcccgataaa aagaacaaat aatttctctt ccaattcgtt aaaacgaccg
     3601 actgttcatt ttccataaac atccctcttt tctttttcat tttgtatagc gtttgcacaa
     3661 caatcattat acgggattct tttttcaaaa aatacctttt tccctaaata acaatcgttt
     3721 caatttaaat ttctttactt agaaacaata aatcagcaaa atattaaaaa taaccgctct
     3781 atcttcccat caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga
     3841 cttctcttgg aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga
     3901 tcctttccca cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg
     3961 tgacaataat ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc
     4021 gtcttctgca gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac
     4081 accgacttct tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat
     4141 cttacctata ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc
     4201 agcatccgtc aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt
     4261 ttctaatgat gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc
     4321 tgagactagc gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg
     4381 taagatatct gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat
     4441 tacttcgata tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc
     4501 acgaagcaag tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc
     4561 gattgatcct tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat
     4621 acgtgctgta taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc
     4681 tttggtacgt gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat
     4741 cactgcttgc cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg
     4801 agtgatatcg tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc
     4861 aggttcaccg attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga
     4921 gccagttgca aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt
     4981 gaagacagaa cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc
     5041 ttcggaaatc aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc
     5101 tttacgggtg taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc
     5161 gcggatggct tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac
     5221 atcttgggcc acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc
     5281 tgtatcggtc atccctttac gagccccgtg agttgagata aacatttcca agacggatag
     5341 tccttcacgg aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat
     5401 caaaccacgc attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc
     5461 agacatcatg aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct
     5521 gtcttttgca gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag
     5581 accacgacgg aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc
     5641 ttgtttttca ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga
     5701 atgtttgtac cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa
     5761 tcgtttgaag acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa
     5821 ttcttgctct ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg
     5881 tgtttggacg gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat
     5941 gatctcgttg aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc
     6001 agtaaatggt ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac
     6061 atatccgttg cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc
     6121 ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgagaagg
     6181 agtaacaact ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat
     6241 acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc
     6301 gaaatcggca ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac
     6361 taaaactggt tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa
     6421 tactgggtgt tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc
     6481 gattttacgt ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat
     6541 cacgaatggt ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt
     6601 caagaaaggt ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa
     6661 gttttgacgg aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg
     6721 gttacctggt ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc
     6781 ttcttgcagc atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa
     6841 acgtttcaaa cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt
     6901 tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat
     6961 aacgtccata accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc
     7021 caaacggcgg attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa
     7081 ttcggctgct tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc
     7141 catagcagct tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt
     7201 caacaattgt tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa
     7261 ataaatgatt tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg
     7321 aatcccttta aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg
     7381 ttcacggcga actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt
     7441 ataacggata cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat
     7501 ccgctcacag aacaaccctt cgcgttcagg ttttaaggta cgatagttaa tcgtctcagg
     7561 tttttttact tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg
     7621 catactttcg aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca
     7681 aaccgcaaga cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt
     7741 atcttctgcg gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc
     7801 agctttcttt tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac
     7861 agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat
     7921 atccaagaca cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga
     7981 ttccggtaca cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac
     8041 acgtccgact acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc
     8101 gtaagcttcc agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt
     8161 acctcccaat ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt
     8221 gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc
     8281 aaatggttca cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc
     8341 ttcacgaact gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac
     8401 gtggatacct aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt
     8461 catacgtgaa ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag
     8521 gaatggcata tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc
     8581 ggccatttta tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat
     8641 gtttacgcct ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac
     8701 gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc
     8761 gccaaagatc gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt
     8821 gactttccct acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat
     8881 ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc
     8941 aggtcctaat tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta
     9001 tacgtcatct tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc
     9061 ccaagtcatg aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga
     9121 tggtccgtct gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg
     9181 ttggttgtag cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa
     9241 tgcaccgttg tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc
     9301 atgtttacat aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc
     9361 aacccatggt gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat
     9421 caaggcacgg ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac
     9481 tacttgttta ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc
     9541 actttgagca cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct
     9601 gtttgcttgg gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc
     9661 agtaacacga cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt
     9721 atttactttc gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt
     9781 ttcgatcgga cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc
     9841 gcggtcacga gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc
     9901 gccaagaggg tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt
     9961 gatacttgcc acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc
    10021 ttggatcgac attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg
    10081 gttttgtaaa agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc
    10141 gacattgccg ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc
    10201 tggacgaacc gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg
    10261 ttcagggtct cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac
    10321 accgtcttct gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag
    10381 tgtttccatg acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc
    10441 tggatcaact aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt
    10501 tttgtttact ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg
    10561 agcattcagc aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc
    10621 gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt
    10681 ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga
    10741 accaaatcct aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg
    10801 tacataagag atgtcttttg catcagtttc catttctagc caagctccac ggttagggat
    10861 aacagtagaa ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc
    10921 tggtgaacgg actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc
    10981 ttgttctgtc attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt
    11041 ctcgcggttt gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc
    11101 acgagcttct gctactgtat actttggttc tttcaattca taatctacaa attccaaaga
    11161 tagattgcca ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc
    11221 atctaagaac cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac
    11281 ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc
    11341 agccaagctc ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca
    11401 ataatatttc aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa
    11461 aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt
    11521 ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt
    11581 tttgcaactt catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt
    11641 ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat
    11701 tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa
    11761 cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta
    11821 gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta
    11881 tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt
    11941 ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt
    12001 ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa
    12061 ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc
    12121 tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat
    12181 tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga
    12241 aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc
    12301 ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga
    12361 tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat
    12421 cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga
    12481 aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg
    12541 cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga
    12601 agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa
    12661 agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc
    12721 taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt
    12781 tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt
    12841 caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggca aacaaacagg
    12901 gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc
    12961 gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga
    13021 cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt
    13081 ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc
    13141 actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca
    13201 gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc
    13261 tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt
    13321 cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg
    13381 atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca
    13441 ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt
    13501 gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc
    13561 gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt
    13621 tatatgattt ctaatggatg cattgctctt tctggctcct tacttgcgca aaacaacggc
    13681 tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc
    13741 gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt
    13801 gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat
    13861 tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa
    13921 aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat
    13981 tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag
    14041 gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg
    14101 gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt
    14161 tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc
    14221 gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg
    14281 ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc
    14341 gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct
    14401 cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat
    14461 tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt
    14521 ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta
    14581 tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc
    14641 aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat
    14701 tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat
    14761 cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct
    14821 ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc
    14881 tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca
    14941 aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc
    15001 aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct
    15061 atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa
    15121 aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga
    15181 cttctgaatc tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga
    15241 agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg
    15301 ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg
    15361 aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa
    15421 ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct
    15481 gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca
    15541 ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa
    15601 aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca
    15661 aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata
    15721 aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg
    15781 tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga
    15841 agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag
    15901 atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag
    15961 actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa
    16021 tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat
    16081 cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc
    16141 atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta
    16201 gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg
    16261 catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca
    16321 gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc
    16381 aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg
    16441 ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa
    16501 ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg
    16561 gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc
    16621 tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc
    16681 aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt
    16741 tcctcctttt tatttatcct acttactttt tcctaattct aggataccta cttatatgtt
    16801 actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga
    16861 aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt
    16921 aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga
    16981 gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt
    17041 actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga
    17101 aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt
    17161 gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca
    17221 tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct
    17281 tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa
    17341 ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt
    17401 ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac
    17461 tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt
    17521 tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac
    17581 tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac
    17641 cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat
    17701 cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa
    17761 cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga
    17821 tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt
    17881 tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac
    17941 gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat
    18001 atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag atacatctac
    18061 cacaagtgat agctcagatt catcaacttc aagcgacagt acagatacta cctctaccac
    18121 aagtgatagc acggatacat cagcttcaag cgacagtaca gatactacct ctaccacaag
    18181 tgatagcacg gacacatcag cttcaagtga cagt
//
LOCUS       DAZKNO010000045        17896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000045 DAZKNO010000000
VERSION     DAZKNO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.045"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..997
                     /locus_tag="ACG8MY_001161"
     CDS             185..997
                     /locus_tag="ACG8MY_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0430375.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            994..1794
                     /locus_tag="ACG8MY_001162"
     CDS             994..1794
                     /locus_tag="ACG8MY_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0430376.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1955..3223
                     /gene="rseP"
                     /locus_tag="ACG8MY_001163"
     CDS             1955..3223
                     /gene="rseP"
                     /locus_tag="ACG8MY_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0430377.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3291..5000
                     /locus_tag="ACG8MY_001164"
     CDS             3291..5000
                     /locus_tag="ACG8MY_001164"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0430378.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5212..9564
                     /locus_tag="ACG8MY_001165"
     CDS             5212..9564
                     /locus_tag="ACG8MY_001165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0430379.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9708..10181
                     /gene="rimP"
                     /locus_tag="ACG8MY_001166"
     CDS             9708..10181
                     /gene="rimP"
                     /locus_tag="ACG8MY_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0430380.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10205..11380
                     /gene="nusA"
                     /locus_tag="ACG8MY_001167"
     CDS             10205..11380
                     /gene="nusA"
                     /locus_tag="ACG8MY_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0430381.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11402..11695
                     /locus_tag="ACG8MY_001168"
     CDS             11402..11695
                     /locus_tag="ACG8MY_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0430382.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11692..12003
                     /locus_tag="ACG8MY_001169"
     CDS             11692..12003
                     /locus_tag="ACG8MY_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0430383.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            12016..14322
                     /gene="infB"
                     /locus_tag="ACG8MY_001170"
     CDS             12016..14322
                     /gene="infB"
                     /locus_tag="ACG8MY_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0430384.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14349..14696
                     /gene="rbfA"
                     /locus_tag="ACG8MY_001171"
     CDS             14349..14696
                     /gene="rbfA"
                     /locus_tag="ACG8MY_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0430385.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14804..15308)
                     /locus_tag="ACG8MY_001172"
                     /pseudo
     CDS             complement(14804..15308)
                     /locus_tag="ACG8MY_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15473..17836
                     /locus_tag="ACG8MY_001173"
     CDS             15473..17836
                     /locus_tag="ACG8MY_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFQ0430386.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6112 a         3164 c         3929 g         4691 t
ORIGIN      
        1 aaaacagagg ggcggatcat tccgcttcct ctgtttttat ttatatttct tttatttaaa
       61 aattaaaaaa agaaaaaatt attcagtttt taaataaaaa aacaatgatt tcataggaaa
      121 aacagttaga tattgctata atacttaaga tagtgggaat cttttaagta taggaggaaa
      181 aagaatgtta cgtttttttc cgcaaaaaaa caaatatatt caagaaaaaa gcgaatttca
      241 atttaatacc gatggaccaa ttcctcagca cattgccatc atcatggacg gcaatggtcg
      301 ttgggcgcaa aaccgtcgtt tgccacgagt tgctggccat aaagaaggca tggaaacagt
      361 aaagaaagtc acaaaaaaag catcaaggct tggagtgaaa gtgttgacgc tttatgcctt
      421 ttcgactgaa aactggaagc gaccaaaaga tgaagtaagt tttctcatgc aactacctgt
      481 cgacttcttt gatacgttcg taccagaatt gattaaagaa aatgtcaaag ttcatgtcat
      541 gggttatgaa aacgtattgc cagaacatac gcaagatgct gtaagacgtg caattgagca
      601 aacaaaaaac aatactggaa tggtattgaa ttttgctttg aattatggaa gtagagcaga
      661 gatcgtgact gctgtccaag aaattgccga agaagtagca aaaggagaaa tacacgcaga
      721 agaaatcgat gatgaattga ttgctaagca tttaatgaca ggtttcttgc ctaaagagtt
      781 acaagatcct gagctgatga tccgtacaag tggagaagag cgaattagta attttctgtt
      841 atggcagatc gcatatagtg agctgtattt tacaaaagcg ctttggcctg attttgatgg
      901 tgcgcatctt gaagaagcaa tcgcctctta tcaaaatcga gaccgtcgat tcggcggtgt
      961 aaaaaaaaca gaagatgaga aaggggatca gtcatgaaac aacgtgtgat cacagcagct
     1021 gtggcgctag tcttgtttat tccgattata tggatcggag gcatagcggt tgagcttaca
     1081 gcagcaatct tagcggtagt aggagtttac gagctgttcc ggatgaaagg attgacactc
     1141 ttaagttttg aaggagtttt atccgccgtt ggtgcagtgt tacttgtttt gccaaaagaa
     1201 agatggtttt tctttcttcc agagaatgta agtacattta tgttatttta tcttatagtt
     1261 atgattcttt taggaacatc agttatttct aaaaatacgt atacgattga tgaagcaggt
     1321 tttcctgtgc ttgtcagctt gtatgtggga ataggtttcc agaattttgt caatgcacga
     1381 acagaaagct tggctgtttt gatatttggt ctatttattg tttgggcaac tgatatcgga
     1441 gcatatatgg ttggtcgacg ttatggtcag cataagttaa gtcctgatat ttcgcccaac
     1501 aaaacgattg aaggtgcgct cggtggtatc ggtagtgctg ttgtagtcgc atttctttat
     1561 ttattgctat acccagcaaa agaactattc aatcatggta tgtttgtcat gcttctgatg
     1621 acgattattt tttcagcagt cggacaattt ggtgatttgg tcgaatctgc aatcaaacgt
     1681 cattatgatg tgaaagactc gggaaatatc ttaccaggcc atggtggaat cttagaccgt
     1741 ttcgacagtt tgctgtttgt atttcctatc atgcatttat ttggcattat ttagtaagta
     1801 tacctgcgtt tctttgtgaa tttcataaaa gacgcaggtt tttatttttc attatattga
     1861 tactattggt gtctttggta tactatggta aaattatagt gttttatcgt tgttttataa
     1921 aaaacacaaa aaacaagtaa aggagaaaaa agctatgaaa acgattctga catttatcat
     1981 cgtttttggt atattagtga ttgttcatga gtttggtcat ttcttctttg cgaaacgatc
     2041 aggaatcctc gtccgcgaat ttgctatcgg tatgggtccg aaaatttatg ggcatcaagc
     2101 aaaagacggg acaacatata cgcttcgcct attacctatt ggcggttatg ttcgaatggc
     2161 aggcaatggg gatgatgaaa cagaaatggc accaggaatg cctctttctc ttttactaaa
     2221 ttctgacggc atcgtagaga agataaattt aagtaagaaa atccaattga caaatgctat
     2281 cccgatggaa ttgagccgtt atgatttaga agatgagctg actatcactg gttatgtgaa
     2341 tggagacgaa acagaagtag taacctaccc tgttgatcac gatgcgacga ttattgaaaa
     2401 cgacggaaca gaaatcagaa ttgcgccaaa agacgtacag ttccaatcgg ctaaattgtg
     2461 gcagcgtatg ctgaccaact ttgctggacc catgaataac tttattttgg caatcgtgtt
     2521 attcatcatt ctggcattta tgcaaggtgg tgttcaagtg acgaacacta atcgcgtagg
     2581 agagatcatg cctaatggag ctgcggcaga agcaggctta aaagaaaatg atgaggtagt
     2641 cagtgtggac ggaaaagaaa tccattcatg gaatgatctg acaacagtca ttacgaaaaa
     2701 tccaggtaaa acattagact ttaagatcga acgagaaggt caggtgcaat ctgtcgacgt
     2761 gacaccgaaa tctgtagagt caaatggcga aaaagttggc caacttggta taaaagcccc
     2821 aatgaataca ggttttatgg ataagatcat tggagggacg cgtcaagctt tcagcggttc
     2881 attggaaatt ttcaaagcct taggatcact atttacaggt ttcagtttag ataaattagg
     2941 cggacctgtc atgatgtacc aattgtcttc agaagcagca aatcaaggtg taacaactgt
     3001 tattagtttg atggcgcttc tttcaatgaa tctcggaatc gtcaatctgc ttccgattcc
     3061 tgccttagat ggcgggaaat tagtcttgaa tatttttgaa gggattcgcg ggaaaccatt
     3121 aagtcaagaa aaagaaggaa tattgacatt agctggcttt ggatttttaa tgttgttgat
     3181 ggtgctagtt acatggaacg atattcaacg attctttttc tagaaaaagt tgacaaatag
     3241 ggtataataa caaacgataa ctattttgag gaaataaagg agtatataga atgagacaat
     3301 caaaaatgtt gattccaaca ttacgcgaag ttccaaatga tgcagaagta ttaagccatc
     3361 aaatcttgct aagagctgga tatatccgcc aagtatcagc gggtatttat tcatatctgc
     3421 cattagcaaa tcgtgtattg gaaaaactga aacgcattat gcgggaagaa tttgaaaaaa
     3481 tcggagcggt agaaatgctg atgcctgcca tcttgccagc agaactttgg gaagaatccg
     3541 ggcgttacga aacatatgga ccaaacttgt atcgtttcaa agaccgcaat gatcgaaaaa
     3601 tgatccttgg accaactcac gaagaaactt ttactgattt gatgcgtaat gaaatcaatt
     3661 catataagaa attaccttta aatctttatc aaatccaacc taaatatcgt gacgaaaaac
     3721 gacctcgctt cggtttgctt cgcggaagag agtttatcat gaaagatgca tattctttcc
     3781 atgcaacgga agaaagtttg gacgagacat ataaagatta cgaaaaagcc tataccgaag
     3841 tattcaagcg ttgtggctta gaatttcgct cgatcatcgg agacggcggc gcaatgggcg
     3901 ggaaagactc aaaggaattc atggcgatct cagaaatcgg ggaggatacg atctgttatt
     3961 cgactgaaag tgattatgct gcaaacttgg agatggctac aagctactat gtatcaaaaa
     4021 aatcgcatga aacacagctt gaattggaga aagtagctac acctaacgta aaatcaattg
     4081 acgaagtagc agatttcttt tctgttgaac ctcaaaaaat catcaagtct gtattgttca
     4141 ttgcggatga acaacctgtc cttgttttgg tacgtggaga ccatgaagtc aatgacgtaa
     4201 aactgaaaaa ctttttatct gctgactttt tagaagaagc aacagaagaa gaagcacaaa
     4261 aatatctagg tgctgagttt ggttctgtcg gaccagtcgg tgtttcagat gacgtaaaag
     4321 tatatgcaga ccgttatgtc caagatttag cgaatgccat tactggagcg aacgaaacag
     4381 gattccacta taaaaatgtg aatccaaaac gcgatttcac tgtgctttct tatgaagatc
     4441 ttcgttttgt acaagaagga gatccttcac cagacaacaa cggtgttctt gcctttactc
     4501 gaggaatcga aatcggacat atattcaaaa tcggcactcg ttacagtgaa tctatgggag
     4561 caactgtact agacgaaaat ggacgagaaa aatttgtgat tatgggctgt tatggtatcg
     4621 gtgtaagtcg tttattatca gcaatcgtag aacaacattc agatgaacaa ggaatcaatt
     4681 ggccaaaagg aattgcgcca tttgatctgc atatcgtcca aatgaatcta aaagacgaat
     4741 accaaacaag cctgacgaat gaactagaag aagcgatgac aaaagctggc tatgaagtac
     4801 tggttgatga cagaaatgaa cgcgcaggag tgaaattcgc ggattctgat ttgattggct
     4861 gtccaatcag aatcactgtc ggtaaaaaag cagtggatgg aatcgtagaa gtgaaaatca
     4921 agaaaactgg cgaaatggtt gaagtacgta aagaagaatt ggcgaatact ttaccaatcc
     4981 tcttgaacca aggcgaataa taagcgttaa aacacagaaa ataaaaatag ctgaatcagc
     5041 tgtttttgac aatctgatga ttcgctttta aaattggaac tgcgatttga atcgcagttc
     5101 caatttttta atgtaaaact cagaaaaaac gagtatacta aaaggtactg aaaaagaaga
     5161 ggataagatg cgatcaattg cattgtttct catcagaaag ggcgtatatc tttgtcagaa
     5221 aaacgagatt tatttgaaaa actgttggaa caaatccaac tagaggaagc agaaaaaaat
     5281 catccactgc ttactgctgg tgaaatggat agtgtagtgg tccatcgtaa atcgcgttta
     5341 tgggaattta cgctacattt ttccaaaatt ttgcctatta tgctatatcg gtcattgact
     5401 cagcatctga ctatcgcttt taaagatatt gcacaagtca aactgaatat cttagcagat
     5461 gaccagacat tcgatgaaca gctcttgcaa gattactggg cccaagcttt ggaaaatcag
     5521 caatgcgata caccactagc acagcaagta ttgaaaacgc aggtacctgt gataaaagat
     5581 cgtaaagtca tcttgccaat cgacagtaca ggagctattt cttatttgaa gcaacaatac
     5641 ttgccattag tagaagagtt gttcgtcagt tacggattcc caaaatttcg tatcgaacca
     5701 gaagtggatg agcagcaagc ggaacgtgta ttgaaacttt ttgaagaacg caaacaagaa
     5761 caggcagaag cctttatgaa gcaagcagct gaaagtctag ttgtccacga acaaaagaaa
     5821 aaggaaagaa aagagcaatc gccagctctt gaaggaccca tccagctagg acgaaatatt
     5881 ccagccgatg aaccaacgac accaatgatc agtatcgttg aagaagaacg aagagtcaca
     5941 cttgaggggt acgtattcga caaagaagtc agagaactac gttcaaaacg caagatcttg
     6001 actttgaaaa tcacagatta tacttcatca tttatcgtca aaaaattctc gaacggcgaa
     6061 aaagatgaac aagtatttga tgcgatcagt gtcggtagct ggttaaaggt acgaggaagt
     6121 attcaagaag atacgtttgt ccgtgatttg gtcatgaatg cgcaagatat catagaagtc
     6181 aaacatacac ctcgcaaaga ttatgctcct gaaggggaaa aacgagtaga gcttcatgtt
     6241 catagcaata tgagtacgat ggatgcaaca aacagcatct ctgatcttgt ggcacaagca
     6301 ggaaaatggg gacaccgagc tatcgccatt acagaccatg gtggtgcgca agcctttccg
     6361 gaagcacata gtgctggaaa aaaagcaggt gtgaaaatct tatacggcgt ggaagcgaat
     6421 gtcgtagatg atggtgtccc aattgcttat aatgatgcac atgaagcatt gagtgaagcc
     6481 acatacgtcg tatttgacgt ggaaacgaca ggattgtctg cagtatatga cacgatcatc
     6541 gagctagccg cagtcaaaat gtataaagga aatgtgatcg aaagctttga tgaatttatc
     6601 gatccaggac atccattgtc acgaacaacg gtagatctga ctggtatcac ggatgaaatg
     6661 gttcgagggt caaagtcgga agaagaagta ttgcggatgt tccttgaatt ttcaaaagat
     6721 acaattttgg tagcccacaa cgcggctttt gatatggggt tcttgaatac tagctacgca
     6781 aggtatggta ttcctgaagc ggccaatcca gtcattgata cgttggaatt agctagatat
     6841 ctgtatcctc aattcaaacg tttcggatta ggtgtgttat ctaaaaagtt tggtgttagc
     6901 ttggaacagc atcaccgagc aatctatgat gctgaagcaa ctggtcactt agcatggatc
     6961 tttgtgaaag aggcaatgga caatcataac atgctttatc atgatcagct aaatgagcat
     7021 atcggagaag gagattctta caaaagggct cgacctttcc atgtaacgat tttagcaaaa
     7081 aatcaggcag gactgaaaga tttgttcaaa ctgatttcaa tgtcgaatgt cgaatacttc
     7141 gaacgcgtcc cacgaatacc acgctcccaa ttgaaaaaga tgcgtgaaaa tctattgatc
     7201 ggctcagctt gtgataaggg agaaatattc gaagccatga tgcaaaaagg ggtagaagag
     7261 gcaagaaacc gagcaaaatt ctatgattat attgaagtta tgccaaaagc ggtctatgcg
     7321 cctttgatcg agcaagaatt ggtaaaaaat gaacatgatt tggaagaaat tatccaaaat
     7381 ttggtagaaa tcggaaaaag tttggataaa atcgttgttg caacaggtaa tgtacattat
     7441 ctgaacgaag aagatgccat ttaccgaaaa attttgatca attctatggg cggagcgaat
     7501 ccgttgaatc gtcatagttt gccggatgtc catttccgaa caaccgatga gatgctaact
     7561 gcatttcact ttttagggga agaaacagcg aaagagatcg tagtagaaaa tacgaataaa
     7621 atcgcagata tctgcgaaga agtgattcca gtcaaagatg aactttatac accgaagatt
     7681 ccaggttcag aagatgaaat atcagaattg agttacacaa aagcaaaaca aatgtacgga
     7741 gatccgttac ctgagatcat tcaaaaacga ttagaaaaag agttgaattc tattaatggt
     7801 aatggcttct cggttattta tctgatcgcc caaaaactag tgcataaaag taatgaagat
     7861 ggctatctag taggttcacg tggctctgtt ggctctagct ttgttgcgac gatgacagga
     7921 atcactgaag ttaatccatt agcccctcat tattattgtc cagagtgcca atattccgag
     7981 ttttttgaag atggtacgta tggttctgga tttgacatgc cagaaaaaca atgtccaaaa
     8041 tgtggtgcac gcttaaacaa agacggtcat gatattccat ttgagacatt tttaggattc
     8101 catggagaca aagtgcccga tattgatttg aacttctcag gtgattatca agcagaagcc
     8161 cataactata cgaaagtatt gtttggcgaa gactatgttt accgtgccgg aacgattggt
     8221 acagtcgccg acaaaacagc atatggctat gtaaaaggat acgaaaggga caataatctg
     8281 cagtttcgaa gtgcagaagt cgacagatta gcaaaaggcg ctactggagt caaacggaca
     8341 accggccagc atccaggggg gatcatcgtt attcccgatt atatggatgt ctatgatttt
     8401 acgccgatcc aatatccagc agacgatcaa aattcagaat ggaaaacgac tcattttgat
     8461 ttccactcga ttcacgataa tgtattgaaa ttagatattc tagggcatga tgacccaact
     8521 gttattcgga tgcttcaaga tttatcaggg atcgacccac aaacgatccc tacggatgac
     8581 ccggaagtga tgcggatatt tgccggtcct gaagtacttg gagtaagtca ggaacaaatt
     8641 tattccaaaa cagggacatt aggtattcca gaattcggca ctcgttttgt tcgagggatg
     8701 ttagaagaaa cgcatcctac aacttttgca gaactactgc aaatttccgg actatctcat
     8761 ggtacagacg tatggttagg aaatgcggaa gaattgattc gacgaggaga tgcgacactg
     8821 gcggaagtaa tcggctgtcg tgatgatatt atggtttatt tgatccatgc tggattagat
     8881 agcgggatgg catttaaaat catggaaact gttcgtaaag gacaatggaa caagatccct
     8941 gatgaattgc gagaaaccta tctatcggcg atgaaagaaa ataatgtgcc cgactggtat
     9001 atcgattctt gttcaaaaat caaatatatg tttccgaaag cccatgctgc agcttacgta
     9061 ttgatggctc ttcgtgtcgc ttatttcaag gtttatttcc ctattttgta ttactgtgca
     9121 tacttttctg tacgtgcaga cgactttgat ctagtctcca tgtgcaaagg aaaagatgct
     9181 gtgaaacaag caatgaaaga aatcacggac aagggattag atgcttccgt aaaagaaaag
     9241 aatcaactga cagtgctgga gttagccaat gaaatgctgg aacgaggatt caaatttgga
     9301 atgattgatt tgtataaatc ggatgccgtt aactttgtta ttgaaggtga tacgttgatc
     9361 gcaccattta gagctgtccc aagtttggga accaacgtgg cgaaacaaat cgttgaagca
     9421 agaaaagacg gtccattttt atccaaagaa gatcttgcca cccgcggtaa agtttcaaaa
     9481 acattgatcg aatatatgaa cgacaatggc gtattgaaag atctaccaga tgaaaatcaa
     9541 ttatcgttat tcgatatgct ataagcatct gttttcctgt ctgcttgtaa ttttaagcag
     9601 agtatggtat agttcttata ggtaatgata ctaactgtga gtgagcggat ttttcgctca
     9661 ctctttttaa tggaattacg cacttaattt ttaaggaggc gaaacttttg agtagcgtcg
     9721 ttgaaacagt cacagaaatg gtgacaccga ttttagatga acaaaatttt gaacttgtcg
     9781 aagtcgaatt tgtaaaagaa ggaaaaagct ggttcttacg agtatttatt gacaaagaag
     9841 gcggtatcga tatcgaagaa tgcgcctttg tcagtgagaa actgagtgaa aaacttgatg
     9901 cagcagatcc agatccgatt cctcaagctt atttcctgga ggtatcctcc ccgggagcag
     9961 agcggccatt gaaaaaagaa agcgactatg aaaaggcact tggtgattat gtccatgtct
    10021 ccttatacca accagtagat ggcgaaaaac aatatgaagg atttcttcaa tcatttgatg
    10081 ctgaacagct tacattgaaa atacgcataa aaacacgaga aaaagaattg acttttgacc
    10141 gaaaaaatat tgcaaaagct cgcttagcta tccaatttta atcacggaga ggaatcagaa
    10201 aaaaatgagt aaagaaatgt taaatgcgtt agacgcttta gaagctgaaa aagggatctc
    10261 aaaagaaatc gtgatcgatg ctttagaagc agcattggtt tctgcataca aacgtcacta
    10321 tggccaagca caaaacgtcg aagtggaatt tgatcagaaa aaaggaaaga tccatgtcta
    10381 tgcggtaaaa gaagtaacgg aagaagtgat ggattcacag ttggaagtct cattgaaaga
    10441 tgcactttta atcaatccag catatgagat cggtgatacg atccgttttg aagtaacacc
    10501 aaaagatttt ggacggatcg ctgcgcaaac ggctaaacaa gttattttac agcgtgtacg
    10561 tgaagcagaa agaacgatta tttataacga attcagtgct tatgaaaaag acatcatgca
    10621 aggaatcgtc gaacgccagg ataaacgtta tatttatgtc aatcttggta aaattgaggc
    10681 agttctttct aaacaagatc aaatgccaaa tgaattttat caaccacatg atcgaatcaa
    10741 agtttatgtt tcccgagtag aaaatacgtc taaaggtccg caagtatttg ttagccgcag
    10801 ccatccagac ctattgcgtc gcttatttga acaagaagta ccagaagtct atgatggaat
    10861 cgttgaaatt gtcagcatcg cccgtgaagc tggcgatcgt tcaaaagtgg cagttcgctc
    10921 aaatgatcca aatatcgatg cagtcggtac atgtgtcggg ccaaagggac aacgagttca
    10981 agcaatcgtc aatgaattaa aaggcgaaaa catggatatc gtcgaatgga atgaagatcc
    11041 agctgtattc atcgcaaatg cattgaatcc tgcacaagtc atggatgtga tcttcgatga
    11101 gacaaatcca aaggcctgca cagttgtcgt tccagactac caattatcgc tagcaatcgg
    11161 taaacgcgga caaaatgcac gtttagctgc taaattgact aaccataaaa tcgatatcaa
    11221 atcagaatct gacatgacgg aattttatga aaatcaagaa caacaaaaag aaacagaaga
    11281 actgcatgat gaagcaatcg ttcagtcaga tttaacagac gatgaatatg aaacaattgc
    11341 attcaacaat gaaacggtcg aagaaaaacc agaagcttaa ctttagtaag gaggcaaaga
    11401 gatgaaacaa aggaaaattc ctttacgcaa atccgtggtt tcaggtgaaa tgaaacctaa
    11461 aaaagagatg gtccgaatca cccgttcaaa agaaggggaa gtatcgatcg atccaactgg
    11521 aaaaatgcct ggacgaggag cgtatgtctc acttgagccg gaagaagtac aacaagcttg
    11581 ggataaaaaa attctcgatc gagtactcga aacaacatta acagatgagt tttatcaaga
    11641 acttcttgac tatgtcactc accaaaaagc acgtaaagag ttatttggaa aatgaataca
    11701 gttaatcggc aaaaagccat gaatctcatc ggtctagcga tgcgcgcggg aaaactaatc
    11761 acgggtgaag aactgaccat tgctgaaatc cgcaaacaaa aagcaaaatt ggtgtttgtg
    11821 gcaaccgatg caagtgagaa tacaagaaaa aagataaaag ataagagttc atattatgaa
    11881 gttccttgct ttgagctatt ttctgaagca gaaatcacgc agatgatcgg aaaacctcgc
    11941 aaggtattcg gaataatgga tgccggattt gcgaagaaga ccaaggagct aattgaaggt
    12001 taggaaggtg attgcatggg caacaaaaga atctatgaat tagcaaaaga actcaaaaaa
    12061 tctagtaaag acgtcgtgga aaaagcacaa caacttggca tcgatgtgaa aaaccatatg
    12121 ggtgcgatta ctagcagtga tgagaagaaa ttgcaacaag cgttcaaaaa cccaagcacg
    12181 aaacaacaag ctgcacaaca agcaagcaga aaaccagcca atcaacagcc gaacgatcaa
    12241 cgtaaaaaag aaacaacgaa caatcaacaa aaaaataaaa ataaccgcag ttatcaagat
    12301 cgcggtcaag ggagcggtca agtgaatcaa ggaaaaaaac caacaacaaa ccaaaataat
    12361 acgggaaata ccagcagcca aaatcgtcaa ggaacgagcc aaggcggcaa tcaaaaccgt
    12421 actaacaata acaacaaacc aaacaataac aatcgcggca aatttaataa taataaccgt
    12481 aatcgttata ataaaaaagg taaaaaaggc aaacagcaac aatcaactaa accagctgta
    12541 ccaccacgca aattccgtga attgccagat gtattagagt atactgaagg tatgaacgta
    12601 gcagatattg cgaaaaaaat tcatcgtgag ccagcagaaa tcattaaaaa attatttatg
    12661 cttggtgtca tggtcaatca aaaccaagca ctggataaag atacgattga actgttagcg
    12721 acggactatg gcatggaacc acaagaaaaa gtccaagttg acattgctga tatcgataaa
    12781 ttcttcgagg cagaagaagt cgatccagat aaattagtct cacgtccgcc agtagtaacg
    12841 attatgggac acgtagacca cgggaaaaca accttattgg atacattgcg ccattcacgt
    12901 gtaacaagcg gcgaagccgg cggaatcaca cagcatatcg gtgcctacca gatcgatatt
    12961 gatggtaaac caatcacttt cttggataca ccaggacatg cggcctttac aagtatgcgt
    13021 gcacgtggag caagtatcac agatatcacg atcttagttg ttgctgcaga tgatggcgtc
    13081 atgccacaaa cagttgaagc aatcaaccat gccaaagctg caggcgttcc aatcatcgtc
    13141 gctgtaaata aaattgataa accaggtgca aatcctcagc atgttatgca agaattaagt
    13201 gagtatgaat taattcctga agcatggggt ggagaaacga tttttgttga gatctcagcg
    13261 aaattcggcc aaaacatcga agaattgctg gaaatgatcc tgttagtcgc agaagtagaa
    13321 gatttaaaag cagatccaac acaacgcgca atcggaacag tgatcgaagc acgtcttgat
    13381 aaaggtaaag gtcctgtgtc aaccttactt gtccaagaag gaacacttca cgttggtgac
    13441 ccaatcgttg tcggtaatac ttacggacgt gtccgtgtaa tgacaaacga tcttggccgt
    13501 cgcgataaag aagcaggacc tgcaacgcca gtagaaatca ctggcttgaa tgatgtacct
    13561 caagcaggtg accgtttcgt agtcttcgaa gacgaaaaga cagcacgtgc agcgggtgaa
    13621 gaacgcgcaa aacgtgcatt attggaaaac cgtgctgcca caagccgtgt gactttggac
    13681 aacttatttg aaagcctgaa agaaggcgaa ttgaaagaag ttaacgtaat catcaaagca
    13741 gatgtacaag gttcagcaga agcactggct gcatcattga agaagattga tgtggaaggt
    13801 gtccgtgtaa aaatcgtcca ttcagctgtc ggagcaatca atgaaagtga tgtaacctta
    13861 gcagcagcca gcaacgctat tatcatcggt ttcaacgttc gtcctacacc gcaggcaaaa
    13921 gtgcaagctg atactgaaga agtcgatatt cgtcttcacc gtatcatcta taaagccatc
    13981 gaagaaatcg aaactgcgat gaaagggatg ctagaccctg aatttgaaga aaaaatcacc
    14041 ggtcaaatga tcgttcgtga aaccttcaaa gtttctaaag ttggtacgat tgcaggtgcc
    14101 tatgtaacag aaggatatat tcgtagagat agcggtgtcc gtgtaatccg tgacggaatc
    14161 gtaatctatg aaggccaact tgcaagtctg aaacgattca aagatgacgt gaaagaagtc
    14221 aaaatgggtt atgaatgtgg tgcgatgatt gaaaaattca atgacatcaa agtagatgac
    14281 gtaattgaag gatttatcat ggaagaagtc aaaaatgact aaaaactgat aaaaggaggc
    14341 caatcattat ggctaactac cgtgatcgcc gagtcggtca agaaatctta aaagaagtca
    14401 atgatatttt gcgcaaaaag gtccgtgacc ctcgtgtgga gaacgtaacg atcacagatg
    14461 tgaaagttac gggggactta cagcaagcaa cgatctatta cagcatttta tctgacttag
    14521 catcagataa gaaaaaagca caagatggat tgaataaagc atcaggattg attcgtcgag
    14581 agttagggca caatctaagt atttataaga cacctgaatt gttctttgaa cttgatgaat
    14641 cagttgtcta tgggaaccat atcgatgaat tgcttcgcga cttgaacaaa gactaatcaa
    14701 aaaatcccgc aaatcctgtg aaatcagggg ttgcgggatt tttttgtttt cattaagacg
    14761 tattacctaa gagttccatg actttagtct ggtagcgtgt cagctaacca aaaaagagaa
    14821 aaagaaaagt aaaaaattcg actttctttt ttctatagta tataaatttt tgcatataga
    14881 taaagatcaa gataccgata acggaagcta gattgtaatc tattttagtg acagtcaggt
    14941 atagccaagt aactagaaga tcagtggaac ctgcaccata aaagtttgag tttgtcggtc
    15001 cgttgacagt taaaagaaaa atcacgttga agttattgat atttccgatt acgccgcttc
    15061 gatttggtca cgatccaaat tttggataat ccctgtagaa attagcatag tagctgggat
    15121 accaattcac atattgacaa agataacaga gacttttaca taaaacggat caatcaggaa
    15181 aggaattgct cgatccagcc ccagttttcc aacaatgtgt taactggcct agcgtcattc
    15241 agcaaattat gcatgatgag gagcgaaatg aactgcggaa cagccattgt gataacgaca
    15301 agttttttgg atcaatgtaa aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac
    15361 ctgttcattt ggctagatat aaaatccaaa caaatgaaca gaagtgctta ttaggtttca
    15421 attgggagta tctgcgctag aataagagta ataatgaaaa ggagtgttat gtatggatta
    15481 ttctagtaaa gaatactttg ataaaatgac ggcttggtgg cgtgctgcta attatctttc
    15541 tgttgggcaa ctttatctta aagataaccc cttactgaga cgtacactga aaccagaaga
    15601 tgtcaaaaaa catccaatcg gtcattgggg aacgattcct ggccaaaatt ttatttatgt
    15661 ccatttgaac cgagtcatca ataaatatga tttgaacatg ttttatatcg aagggccagg
    15721 acacggcgga caagtcatgg tatcgaatgc ttatttagat ggaagttaca cagagatcta
    15781 tccagaagta acggaagatg agacaggaat gcaaaaatta ttcaagcgat tttcatttcc
    15841 tggaggcatt gcttctcatg cagcaccaga aacaccaggt tctatccatg aaggcggaga
    15901 gcttggatac tctctttcac atgcagtcgg agctgtatta gacaatcctg aagtaatctc
    15961 tgctgtcgtg attggtgatg gtgaagcaga aaccggtcct ttagctggaa gctggttctc
    16021 aaatgtcttt atcaatccag tgatagatgg agctgtttta ccgatccttc atttaaatgg
    16081 tgcaaaaata gccaacccaa cgatcttggc acgtaaatca gatggagaat tagcaaatta
    16141 ctttaacgga cttggctggg agcctttctt tattgaagga aatgatccag aaaaattaaa
    16201 tcctgttatg gctgaaaaaa tggatcaagc aatcgagaaa atcaaatcca tccaaaaaga
    16261 agctcgcctg aagacagcaa cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg
    16321 cacaccaaaa ggatggaccg gtccaaaaga atgggacggg gaaccaattg aaggaacctt
    16381 ccgagcacac caagtaccga ttcctgtaga tcaagaacat atggatcatg cagatgcttt
    16441 actccgttgg ctgaaatcct atgaaccaga aaaattattt gatgcacaag gacgtatttt
    16501 agaagaaatc agagaaattg cgcctactgg tgaccaaaga atggcaaaaa atcctatcac
    16561 caatggtgga atcgatccaa gaccgcttat tatgccagat tggaaaaaat atactttgca
    16621 atttgaaaaa ccaggctccg ttaaagcaga agatatgacc gaacttggta aattcgttcg
    16681 cgagatcatt gagaaaaatc cagaaaattt ccgtatattt ggaccagatg aaacaaaatc
    16741 aaaccgattg aatcaagttt tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc
    16801 agaaaatgat gaatggcttt cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca
    16861 ccaagatgaa gggttcttag aagggtacgt tctgactgga cggcatggtt tttttgcaag
    16921 ttacgaatca ttcttgcgag tggttgactc tatgttgact cagcacttca aatggatacg
    16981 gaaatcacat gacctttctt ggcgaaaaga ttatccttcg ctgaatctca ttgcaagctc
    17041 aacagtcttc cagcaagatc acaatggata tagccaccaa gatccaggaa tattgacaca
    17101 tttggcagaa aagaaggcgg agttcatccg tgaatatctg cctgcggatg caaatacatt
    17161 gctcgcagtc atggataaag cattccgctc gtcagaaaaa atcaatttga tcatctcttc
    17221 caaacatccg cgtgctcaat tttactcagc agaagaagca gccgtcttag tgaatgaagg
    17281 cttgaaaatc attgattggg cttcgacagc aaaagaggag gaacctgaat tggtcattgc
    17341 tgctgcagga acggaatcta acttagaagc attagcagct gtaactttgc tgctggaaga
    17401 attcccaaaa ctaaaaattc ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc
    17461 aagtcaagat ccgcgtgggt tgagcgatga agaatttgat caatatttta caaaagacaa
    17521 accgatctta tttgctttcc acggttatga aacattggtc cgcacgattt tctttgatcg
    17581 tcataaccat catttaatga ttcatggcta caaagaaaat ggagatatca cgacaccatt
    17641 tgacatgcgt gtagtaaatg aacttgatcg ttatcatttg gcaaaagacg cagctttaaa
    17701 aatcaaagga agtcaagcag aagattttgc ggaaaaaatg gatcagaaat tacaagaaca
    17761 tcaaaactat attcgtgaaa atggtattga tctgccagaa gtattggatt ggaaatggaa
    17821 aaatcttgac caataaaatg ataaaatcga tcccgcaaaa cagaattttt tacattgtat
    17881 tatttgtcaa attaag
//
LOCUS       DAZKNO010000046        17173 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000046 DAZKNO010000000
VERSION     DAZKNO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.046"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..441
                     /locus_tag="ACG8MY_001174"
     CDS             4..441
                     /locus_tag="ACG8MY_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430387.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            536..1420
                     /locus_tag="ACG8MY_001175"
     CDS             536..1420
                     /locus_tag="ACG8MY_001175"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0430388.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1644..4811
                     /locus_tag="ACG8MY_001176"
     CDS             1644..4811
                     /locus_tag="ACG8MY_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0430389.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4879..5376
                     /locus_tag="ACG8MY_001177"
     CDS             4879..5376
                     /locus_tag="ACG8MY_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFQ0430390.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5395..5859
                     /locus_tag="ACG8MY_001178"
                     /pseudo
     CDS             5395..5859
                     /locus_tag="ACG8MY_001178"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5856..6614
                     /locus_tag="ACG8MY_001179"
     CDS             5856..6614
                     /locus_tag="ACG8MY_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0430391.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6700..7440)
                     /locus_tag="ACG8MY_001180"
     CDS             complement(6700..7440)
                     /locus_tag="ACG8MY_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0430392.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7501..8754
                     /locus_tag="ACG8MY_001181"
     CDS             7501..8754
                     /locus_tag="ACG8MY_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0430393.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9076..9303
                     /locus_tag="ACG8MY_001182"
     CDS             9076..9303
                     /locus_tag="ACG8MY_001182"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0430394.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9426..9752
                     /locus_tag="ACG8MY_001183"
     CDS             9426..9752
                     /locus_tag="ACG8MY_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0430395.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9820..10533
                     /locus_tag="ACG8MY_001184"
     CDS             9820..10533
                     /locus_tag="ACG8MY_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0430396.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10843..11694
                     /gene="licT"
                     /locus_tag="ACG8MY_001185"
     CDS             10843..11694
                     /gene="licT"
                     /locus_tag="ACG8MY_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFQ0430397.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            11815..13680
                     /locus_tag="ACG8MY_001186"
     CDS             11815..13680
                     /locus_tag="ACG8MY_001186"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0430398.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13694..15137
                     /locus_tag="ACG8MY_001187"
                     /pseudo
     CDS             13694..15137
                     /locus_tag="ACG8MY_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            15245..15946
                     /locus_tag="ACG8MY_001188"
     CDS             15245..15946
                     /locus_tag="ACG8MY_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0430399.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16072..16584
                     /locus_tag="ACG8MY_001189"
                     /pseudo
     CDS             16072..16584
                     /locus_tag="ACG8MY_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(16982..>17173)
                     /locus_tag="ACG8MY_001190"
     CDS             complement(16982..>17173)
                     /locus_tag="ACG8MY_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430400.1"
                     /translation="QILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNLIIFVHQ
                     LLLKIFLLTINSWICKNIT"
BASE COUNT         5725 a         2889 c         3703 g         4856 t
ORIGIN      
        1 taaatggagt ggttttgcaa atttctttta tttctagaag ttattcacgt atacggattt
       61 gtttttctca caaataaaaa ggcaggaagt acaatggatc atttgtatat aaaatatatt
      121 catcaatttt ccgttttggt gttaggcaca ctaattatag cggggattgt cgcatttatt
      181 attcaaggcc gtgacatatt aacagtacat caagagtttt tcagttatgc atttggtatt
      241 cctctgatcg ctataaggtc tgataacaaa aagtggaaga aacgctatta cattctcttg
      301 ctatttttgt gtttttatct gccacctaag atttttactt tattaggtat acaatgccta
      361 tcattttctt ttatttccta tatcattgtt ttaattttcc tcagtttcat agtatggatg
      421 ttttatcttg tcaaaaaata aatgaatgtt tcatgaacag tacttcatca cagcctaatt
      481 tttttcttta tgctatcgtt atattaaaga aatcaatcag aagggaaaga aagtgatgaa
      541 aaaagtttta ttagtggtaa accccagttc aggcggtgaa caagcaaagg aatttgagca
      601 attagcgata gcaaaattgg aatcggtgtt tgatgaagtg gtcgtcttgc atacaaaaaa
      661 agcaggggat gcaaaaaact ttactcgcga agcggctacg gaagggtatc atagtgtgtt
      721 tgtcatgggc ggagatggaa cagtcaacga aggaatcagt ggaatcgctg aacaagaaca
      781 tcggccaaat ttcggatttt tcccattggg tacggtgaat gacttggctc gagcattagg
      841 gataccgcta gaacctgagg aagcgatcaa tcatttttcg attgaatcag tcaagccatt
      901 ggatattgga aaaataaatg acgactattt tatgaatgtc gtcgcaattg gctctatccc
      961 tgaatcaatc aatgatgtag atgcggagaa gaagacgaaa tttggaaaac tagcttattt
     1021 catgtcagga atcaaacagt tagcttccac acaatcctac tcgtttcacg tagaagttga
     1081 tggaaagaag gaagaaattg agagcagtac cttgctgata ggactaacta actcagtcgg
     1141 cggttttgaa acattacttc caaatgcaaa agtggatgat ggtaagctcc atttggttta
     1201 tttaaaagat tcttctttga tcgatacgct taaaacattg ccagacctat taaaaggagt
     1261 agatgaatct acgaaaaatc tagcctacct tacttgtgag caattagttg tgtcattagc
     1321 tgatgaggaa gaattgacga cgaatgttga tggagatgaa ggggataaat tacctgtgac
     1381 aatcaaagta ttgccctcac atttacaggt ttattgttaa aatggaggtt gtgaccgagg
     1441 agttggcggg agaacaccgc ttgttcggga taagggactg tgacaagact tttgtcacag
     1501 cctttttttt gtacaaaaaa gaaatttaca cctaatagaa aaatttttcc ataaaaatac
     1561 atattttaaa tatgaaagta caattggaaa tagattaaaa cgataaaata aaattgtcaa
     1621 aacgtcagag aggatggtgg agaatgaatc aatgtgtaaa aaagctgatt gcgtcgattt
     1681 tgtttgtctt ccctctcttg atggtgacat ccgtttttgc cgatgtagag aaaacaaacg
     1741 aatcaacgca atcagagaat caaagttttg acaagactac tttttcagaa gaatcgatta
     1801 cttctgaaac tacagaatct caaagtgagg cttctactaa taaaaggcaa gaagaaaata
     1861 agacaagtga gacctcagaa gtagctgaac aaacgtctga tagcagtgaa gcggcactgg
     1921 caagagctgt ttcagcggat gggtacagcg aagctgccac aacagaagaa ctggcgcagc
     1981 ttctcgcaga cgaatcagta actaagatcc gactgattca gcctttgact ttagacaggg
     2041 aattagagat caaacgagat atcgttattg attttggtgg attcgctcac aatttcggca
     2101 cacaccatat ttatatcaat gagatacctc agtcgatcga gttccagaat tttaaaggaa
     2161 cagctgcaca tccaggtgga ttgacaccaa atatcgatgg aaatgccatt atcattgctt
     2221 atatgaccga tttttgggga agcagatatc attttacggg agaaataaaa tttaccggga
     2281 cacttgattt gtatgatggg tccaaattag gactgattta cgctcctcgt gcaactgtca
     2341 cattagatgg tgtttccggt gtgttggatg tccaaccagt gaaggaagga ctgaatgccg
     2401 ctccgggtaa agcctatttt tgtagaagct atcaattgaa tgtgatcaat ggtagtcaac
     2461 tttatggacc gtatctaggg aaattctacg gttttctaga ttcaggagat gagactggta
     2521 gtggaaaaac agcgccagga atccatatct taagcggttc aaaggtctct cttgattatg
     2581 atcgggcaga tggcgttagc gatgaaggag aggcagtcga tacgctacct ggtaatgtca
     2641 tgtttgaagt cagtggttcg ggaagcgaat tttcggtaaa tacaaagatc aatattacca
     2701 atgacaacaa tcgcggaatc atccaaatga gaggttcagg atcgaaagtc actgtatcga
     2761 atggtgggaa aatcaccatc aatacagcaa ctacgggcgg atttcgcttg caaggagatt
     2821 ctagtcaaat ccatgtatca agtggtggac aaatctcggt caaaatggct ggagatgacg
     2881 atcaacccgg gaacaatggg atgcgttttg taggaaaagg cctttctttg atcgtagaag
     2941 gagccggatc agttatcgac atcgataagc agagtgggcg ttccagcgca gttcgttttg
     3001 agaatggtac gcaaaagctg acagtaacca atggaggctc attgagggta agaaatacag
     3061 gtgatggcaa atcttacgtg aacagactca atcaaggaaa tcaagcgatc caattttatg
     3121 acgcttctgg ttttctagaa agtccaggtt cagctgattt tacagtcact ggagaaaaat
     3181 caaatatcga tatacgcgca gattccggag caactgtaga tacaactgga gatattgatt
     3241 taaattttac ggcaggtgaa aaaacttatt tagtcatggt cgggaaaaca ggggaagata
     3301 agaccggcat catcagtggg gatgtgttga atgtggactt agaatctcct acctattttg
     3361 atttccaaaa caaacggatt ggaagcgaga ccaatggcat aggcggctgg gtattccaag
     3421 gaaatggaga ttcttcttta aagatcgccc attcagaaat cgctttatgg cgaaaaacag
     3481 gcgcaacatt cgataacttt gaaaaagacc cggattattt ttctgatatg acagacttat
     3541 cgatcaatgg gaccgactta ggcaatttcg tatcaagccc tagtgcaggt ttaacaaatg
     3601 aatttgctca gacaggtgcc ggaatgaaaa attataatcg aatcagtgcc aataatcagc
     3661 tcccgatgat tgaatcctta agagtgccaa cgaatgcaga taaaaaaatc tatgggcatg
     3721 tcgttgttcc cgaaggtgtc gaggcaaagc caagagatgc ttggacaggc gaagtagaag
     3781 tgactttacg tattacttac gcagatacga cgaaatcatc tgtagaaatt actgctctca
     3841 cgcaaggaca aacagatgaa aatgaagggg caggatataa cccatgggga gaaggcgaac
     3901 aaggcggctt gttcgaagtt caagtaccca atgatgagag attaaaaacg gaggatcaag
     3961 tgcaagttgt tgctgctaaa aaagtaagag gtggccaaga agtgacagaa ctggactctg
     4021 ccaaaacgac agtcgatgtg attcctcctg aacctgctaa tttaagtacc acattgatca
     4081 atgcagcttc tcgcacgatc acaggtacag gtgaagcagg agccttggtt tcattgaaaa
     4141 aaaatcaagc tgttttgtca gaaacaacta ttgatgatac aggggaattt gttctttcta
     4201 tcccagaaaa tcaacttttt gcaggagatg tattagaaat tgtattaaac gaccaagcag
     4261 gagaagctac tgctaagggt gtgcagaaca agccaagtac caacgatgaa attgggaatc
     4321 acaatccaag tgatacgcca gttgtttatc atgatgcccc gccttttgca cctgctacga
     4381 agatcgtagt agaaggtggt ctctcatttc ttgccccttc caaactcgat tttggtcaga
     4441 tcaaagcaac tggtttgaca caagaagcat ttggccaaat ggatatacag gaaaaattat
     4501 ggatttatga tcagcgagaa acgaaaaatc cgtggatctt aacgataaaa ttatctcagc
     4561 ctttcacaaa tgatgcagga ttttctatgc aggatgtcct atatttcaag aaaggagagg
     4621 agtatcaatt agtgaatgaa gaagaactgg aaattgtcag aggacaaaat tcaacgaatg
     4681 tgcaagcaga ttattcagaa tatttaaata acggtcgggc gtttaaactt gtcttaacca
     4741 aagaaaatca gatagctggt aattactcag cagagataac atggacatta gcagatgcgc
     4801 cgacagaata aagatcaagg aggatgattg atgaatcgaa tatggaataa aagtcatcga
     4861 aacaactgct ctttacttat gatccttctc cttggattca tcctttggca gacacctgtc
     4921 catgcctccg aattcaattt tgcggtagat tttgaattac cggctaatca gattgataag
     4981 aacaaaacat atataaatgt aaggatggag cctgggaaaa cacaggaaat cccctacacg
     5041 gtctttaacc gaacagataa atcagtaaca gttgagacat tgatccactc cgctacgacg
     5101 aattcaaacg gtgtgatcga atatggagaa tcaaaaagta aatttgatga aacattaagt
     5161 tatgatatga aaaacttagc aaaaacagtg ggagaaataa ctgttcctcc tcaaagtgcg
     5221 gtcactgaga cactttcact gaaagcgcca gaaaaagagc tggctggtgt ggtcgcagga
     5281 gggatcacgt ttcaagaaaa aaaagagaac gtttctaaca aaagtacagt tggtgtcgtc
     5341 aatcagtttt ctcaagtgaa agcaatcttg atttgaaata gcgaaaaaga gtcttcccca
     5401 gatttgaaat taaaaaaagc ttatgcggat caaagcgatg gaagaaatgt gattaaggcg
     5461 aatcttcaaa atccaaatgc ttcttatatg ttcggcttga ctacatcggt tgaaatctac
     5521 aaaaaagaaa caaaagagcc ttacctttca ataaaaaaag aaaaaataga tgtagcaccc
     5581 aatacaaatt ttgatttact tgtcccatta gaaggaaaaa aattagctcc gggacattat
     5641 caagcggttg tgaagggtgc ttggaaagaa aaacgctgga atctccatac cgattttgaa
     5701 ataactgatg atgcagcgga ccaattgaat gaaaaggata ttgatttaaa aaatcagcca
     5761 gaaacatttg actggcgatg gctgatactt gttggagtca ttcttattgg cttatttgct
     5821 ggattattta ttttaaaaga aagaaggaaa aagaaatgaa aaaaacaatt gcattaagta
     5881 ccgttctagc tggaagtcta ttattatgta cacaaagtgt ttttgctgag aataagagct
     5941 cagccactac agaaggaacg atttctgtga ccaaaccaga tgctgcaaat cctgaagacg
     6001 ttgttccagt tcctcctgtt gatccaaatg atcctgatac tccttttaca cctgacgaac
     6061 caaatcaggg aacatccgga ttactgacga ttgaccaagc tccagatctt gattttggaa
     6121 caatcaagtt aggtacagca aaaacagtat atgctgcatt acaaagcgga acggacagta
     6181 ctggagcaca aacacaagta cagaatttcg tacaagtaac ggataaaagc ggaaattatg
     6241 ccggttggaa attatcagtc aaacgaacag aatttattgg tacaacatct gatacgaata
     6301 aactaactgg ttcaaaggtg aaattcaaaa acgccgtgat tcgaaatagc gcaactaata
     6361 gcagtggaat gccatctacc gtttctggaa ctggcgctga aggaatcgaa atcccagcag
     6421 atcaggcagt agatctagta actgcaggag caaatgaagg gattggcaca tggatttatt
     6481 cattaggaga gaatgtggaa caagggagac aatcagtgga attatttgtt cctgtatcca
     6541 attatgctgt agacaactac gtttcaacat tcacatggtc gttaacagat gctcctacag
     6601 aagaggaagc gtaaaatcat atcaacgatt tcagggagtg gagccgattc aaggcccact
     6661 ccctgaaatc tgctatgctt acgtctcagc tcttattcat caactcgaag cagttttttg
     6721 gaagtcatgg aaacgttcct ttgtcttttc aaagatcttt cgaccaacga gtaaggagtc
     6781 accgttgaaa aattctaaca cataatgagc agaatcaatt ttctgcaatt gcgataagtt
     6841 gatacagtaa gattttaaaa gtaaaaagta agcatacttt tctaactctg tcttcaattg
     6901 ttttaaagaa gtattcacta atttatcttc tgtagttgtt ttgaccataa cttggttttt
     6961 caaacctttg atactttcaa tatataaaat ttcccgagga tacaggacga gacttccacc
     7021 tttttgatca ataaagattt tatcgacatt tacgatctgc tgttctttcc aattgatctg
     7081 ttctagcact tgacgaattt gttcagataa attcctgtct tttctgatat atccaatcgg
     7141 aaggattttc cgattgatga tctctactgc aaaattcgta taagcggtga aaaaaacaat
     7201 attactatgt gcattttttt tacgtatttg ttcggcgaca tcaatacctg tgagtgattg
     7261 cttcaagtcg atatcgataa agaaaacatc gtgattatca atagtgccca actggatcaa
     7321 ctgattgatt tgagagatag aagtgagctg cacgatattc tcgatatgat gattgaccaa
     7381 ttctctgatg atccattcgc tgtgaaactg gttatcttct atgataaaat actttttcat
     7441 atgcaggctc ctattaaaaa aatatgtgta cattttgaaa caaaacaatg gaggaataag
     7501 atggtcgcaa cagtgacact ttatttgacc tttactttga ttttagattt aggtatctat
     7561 ttattctttt cgaaacaact gctagtaaaa aacagctttg gttgtatagc tttgatggtg
     7621 gtgacacttt tacttcctat ccctaaagtg agctttcctt acctagttgg cttattattg
     7681 atgtttcttt tttattttac tacgaaaaac ctgtttatca gctgtttgat cagtaatctt
     7741 ttttttgcta tcactggact cagctttttt ctggtttttg atctgatgga ttatttagga
     7801 aagagacatc ttggaacagc aagttttttt gcactactta tcgcatttat tttctatcta
     7861 ttagtaaaaa gtatagacaa acagacgaga tttctcaact tgctttttac ttatccaact
     7921 gaacactatt ttggaggtat ttgtatttta ttaagctggg gcgtgattta cttggtattg
     7981 ggaagtgttc acttccgctc atctaattat ctgcttctat ctcttttgtc gatagtcatc
     8041 agtttttttt ctttgttttt ttccattatg ttgatcagtt tccaagtaaa agaaagacaa
     8101 catcaggaat cgcttcaact gtatcaaata aatgaagaat attaccatca tctagaggaa
     8161 ttcaaacatg attataaaag tctactgttt tcattaaaaa ttactcttca ggaaaataaa
     8221 aaagaaacgt tggaattttt agaaatatta gaagatcatt ctgcagctat ttttgaaaaa
     8281 ccgcaaatac aacagctaac aaatattgta tccccagctg tcaggggtgt ctttttaaaa
     8341 tttctagaaa aagcagaatc ggagaaaatc ccgatccaag tagttattcc tgtcgaagtg
     8401 aaaacaatcc cgattgatct ggttgttttt attcgctgtt tatctatttt tatttctaac
     8461 gcattcgatc atcgtgtagc cgatcagagt ccgattacga tcaatctgct ggaagaaaaa
     8521 gagggatcac gttttagtat agcgaacaag gcaaatccaa cgaatttgac gctatcggag
     8581 atggtcaaaa gaggaaaaac ctctaaaaaa aatggcgggt taggtctata cagtgcgaaa
     8641 aaaatgttgg atgtttacga aaatgcagaa ctaaaaatcg aattttcaaa acagaatcac
     8701 tatttttttg tagaaatgta tctggcatct agaaagagca aatcagccta ctgacttact
     8761 gtcaaaggat aagtttgagg aaagtctaca cggagataga gacaacaaca cagcatattc
     8821 aaaagaaaca gaagcgtgat gaccgttgtc acccttctgt ttcttttgct atgtagttaa
     8881 acaaagtagt tgacacttcc gctacttagt attagtacga aagtatactt catgatacta
     8941 agtagataag aagtgaaaag cggaacccac gatcgtcaaa atgctaactg ctttgcttac
     9001 accagataaa gggaagagca atatcgctta ttttaggcgt ggagaagcaa gcaaatccct
     9061 acgatcacaa aaaaaatgct gacccagcag ttaagagagt tagaacaaga tgaaagtatc
     9121 catcgaaaag cctataacca agtcccgcct aaagtagagt actcgctcac cgaagaagga
     9181 aaatcattaa gagagattct agtagcgttg tctgtttggg aagaagaacg tatcgaaaaa
     9241 caaaaagcct caagggaaga agacacgcta ctgcatacac atggtggcta cctaaattat
     9301 tgattttcta tgacatttat tttaaataaa agaaaacagg agttttttta aaagcatcat
     9361 ctatttttta tgtaaaaaaa tggtatgatt aatcaagtaa gaagtgattg gaagaaggca
     9421 agcaaatgaa tagtatggaa aaagtcaacc agtttagaga tgaacgtaat tggcgacaat
     9481 ttcataacga aaaagatttg gctatctcta tttctttgga agcatctgaa ttgttagaac
     9541 tttttcaatg gaaaacgccg gaagaagtaa aagaaacaca attagaacga atcaaagaag
     9601 aactggcaga tgtattgatc tattcataca tgatggcaga caaccttcac ctggatctag
     9661 atgagatcat tgaagaaaaa ttgatcaaaa ataatttgaa atatcctgta gaaaaaagcg
     9721 caggtgtacg aaaaaaatac acagagttgt agaaatgtgt cattcggctg ttggaaagag
     9781 caaaagaaaa acagtaaaat gagtctggag ggagagaaaa tgaaaaaaaa aggaaaagca
     9841 aaaagatggc tagtcaatat cctcttgttc ctcttgttat tagtaggact agctttgatt
     9901 ttcaatgaac agatcaagga ttattttgtc agagagacag gagataaata tgctatagcg
     9961 aatgtaacaa aagaagatct gaaaaagaat aacgataaag atgtcagttt tgactttgat
    10021 gcggtagaac caatgacgac agaaggggtc atgaggtcgc aaatgagagg aacagatcta
    10081 ccagtgattg ctagtatcgc agtcccttca gtttcaatca atttaccggt tttcaaagga
    10141 ttggataata cgtcactact ttacggagca ggaacattat ctccagatca agaaatggga
    10201 aaaggaaatt atgccttagc aagccatcgt gcaacgaacc ctgaattgct gtttacaccg
    10261 ctagaaaatt tagagatggg tgccaagatc tatttgacag atttagaaaa tgtgtataca
    10321 tacaaaacat tttttaaaga aaaagttgct cctacagata cccaattact gaatgaagtc
    10381 gagggaaaag aaattgtcac actaattact tgtggcgaca tggacgccgt cacgcgacta
    10441 gttgtacaag gagaactgga aagtgttaca tcgataaaag atgcgacaga tgatatgaga
    10501 tctgctttca atctagagac aaaaacattt taggatactg ggacataagt cgttacagat
    10561 taaaaacagc caaaggatat gaatcgataa ggaaaaacca atttgctgaa aaaaagagaa
    10621 acgcaaagag gtgcatctct gatttggttg ttttctagat agacttgtgt tccagttttt
    10681 ttatttttta tatgaaaagg cttgcataaa taaaaattcg atggtatgat gattttgaat
    10741 aagaaatgga ttgttactgt acggcaggca aaaccagaat tcagctaaga aacagtaggc
    10801 gaattctagg tttttttatt tttcttgaga gggcgaagca aaatgaaaat tgataaaatt
    10861 ttgaataaca atgtcgtcat ttcgaaaaat ggattcgggg aagaagtcgt ttgtatggga
    10921 aaaggactag cttttcaaaa aaagattggg gacgagattt cgccagaagc ggtccaaaaa
    10981 gaatttgttt taagagattc ttttgctaaa aaccaattcc agcagctgat ggctgatatt
    11041 ccggcagagg agatcgaatg ggtcaaacaa gtagtcgaac tggctgaaga taaactaaaa
    11101 gttgaatttt cgcctaatat ttatctgaca ttgaccgatc atcttcatta tgcgatcaca
    11161 cgagcaaaag aaaatatcca actgcctaat ccattattat ttgaaacaaa aaaattttat
    11221 ccgaaagaat atcaagcagc gaaagaggct cttttgttcg tgaaacaaaa gacgggtgtg
    11281 gaactttctg aggatgaagc cggttttatt gcctttcatt ttgtgaatag ccaacaagga
    11341 aacgaagaca tgcaagtgac gatgaccgct accacaatgg taagagatat actgagtatc
    11401 atcagtaaat tcttcggcat cgtgttcgat gaagaatctt tgaattatca gcgtatcatt
    11461 actcatctgc agttttttgc ccagcgttat ctcaaagggg aaggatctga cgagcaagat
    11521 gaatttctct acgcattagt tcaaggaaag tatccaaaag cgtttcattg tgtcgaacgg
    11581 atcaatgagt atcttcgaca aacgcaaaaa caggaaatgg ggatcgcaga gcagatttat
    11641 ttaacgattc atattcaaag agtagtaagt gagaaaaaaa gcatccaatc ataaggattg
    11701 ttactgtatg gcaggcaaaa cctggattga aaaaataggt tcacgaatgt ttgtgactgt
    11761 ttttttcaat ctgggttttt attttttaaa taaaaaagaa agaaggaaaa aacaatggat
    11821 aatcaagcag ttggtcgtcg tgtatgggaa gcggtaggag gacaaaagaa tgtcaaaagt
    11881 ttagtacatt gtgctacgcg gttgcgattc agattaaaag atgaatcttt agcagatacg
    11941 caaaagttaa aagaagaccc ggacgtgatc caagtcgtac aaagtggcgg tcagtatcaa
    12001 gtagtgattg gcagtaatgt agcagatgtt tatcaatcga tcgtggatga ggaaggtctt
    12061 gcagtttcgg aggatggaaa agaagagtcc aaaaatattt tgaatcgtct gatcgatatt
    12121 atctctagta tatttacacc ttttctaggg gcgatggcag ccgccggggt attgaaagga
    12181 ttcttatcat tggcaacggt gatgggctgg ctgacagctg atagcggtac ttatcaaata
    12241 ttgtttgctg cagcagatgg tgtctttacc ttcttacctg tcatgcttgc ttttacagca
    12301 gctaaaaagt tcaaagccaa ccagttttta gcagtagcaa ttgctatggc acttgtgtat
    12361 ccagctatca cagcagttgc tggtgcggga gaagctatca gtttctttgg gatcccagtg
    12421 attctttcac catctggtta tacttcttct gtcataccaa tcattctagc cgtatgggtt
    12481 caaagtaaat tagagccatt cgtaaaaaaa gtgattccgc aatttctgca aatgatttta
    12541 gttccgcttg tcgttttagt agtgatggtt ccattaacat ttcttgcttt aggaccaatc
    12601 gggacggttg ccggtaatgc tttaggcggt ctcttcaact cgatttatgg atttagtcca
    12661 atcgtagcag gtctaatcat gggaagttta tggcaagtat tcgtgatgtt cggcatgcat
    12721 cggggattcg taccgatcat gtttttgaat atcgaacaat atgggtttga tgtattgatg
    12781 ccaatgcttc ttccagccat cttggcacaa ggtggggctg ctttagctgt agcattgcga
    12841 acaaaagata cgaaacttcg agcattaggg atttcttcta cagtgacttc gttatttgga
    12901 atcactgaac caactgtgta tggtgtgaca ttacctttga agaaaccatt cattgccgct
    12961 tgtatttctg gaggtattgg tggagctatt atcggatttt ccggagtaaa agctttctcc
    13021 agcagtttgg tcagcttatt gacgattccg actttcatta atacggtgga tggtgtagag
    13081 tcaaatgtga cagtagcagt aatagctaca ggaattgctt ttgttcttgc attcgtaggt
    13141 acattgattt taggatttga cgaacaaaca caagacaatc aattagaaaa caaacatgcg
    13201 aatgcaggag aaccgattac ttcagcacgg cataccttaa aaagtccttt aacgggtaaa
    13261 gtactgccat tgagtgaagt accagaccaa gttttttctt ctggcgtgat gggaaaaggt
    13321 atcgctattg atccagaagt aggagaacta gttgcacctg cagacggaga gatcacgact
    13381 atttttccaa ctggacatgc tgtagggatc acaacgactg acggagcgga gattttgatt
    13441 catatcggga tggatacagt agaattaaat gggaatggct ttgagatctt agtcaaacaa
    13501 ggggatctcg tcaaagcagg agatttattg attcgttttg acatcgaagc aattagagca
    13561 gctggctata gcgtaatcac tccagttgtc attacgaata ctgatgcatt tgccgatata
    13621 cttgaattag accaaaaaga gattattgca aacgaagatg ttctggcaat tgtaaaataa
    13681 ggaggaaatc aggatgcaat cacgtttttc agaaaaattt ttatggggtg gagcaactgc
    13741 tgccaaatca attagaaggg gcttatgatc gagacggaaa aggactatcc gttgcagatg
    13801 ctatgcctgg aggtaagcag cgctttgcga ttatcggcag tgaagaattt gactggacga
    13861 ttgatcaaga aaaatatatt tacccgaatc atcgaggaat cgatcattac gatcgtttca
    13921 aagaagatct tgccttgttt gcggaaatgg gattcaaatg ttatcgtttt tcgattgcct
    13981 ggacacgaat ttttccaaat ggagacgaag gtactcctaa cgaagcggga ttagaatttt
    14041 atgatcagct gattgatgag tgcttaaaat atgatattga acctgtgatc acgatttctc
    14101 attacgaaat gcctcttcat ttggcaaaag aatacggtgg ctggaaaaat cgaaaactga
    14161 tcgacttcta tgaacgattt gctcagacag tattagagag atacagcagt aaagtcaaat
    14221 attggatgac tttcaatgaa atcaattcgg cctttcactt tccagcttta agtcaaggat
    14281 tagtaaaaag caatggagca ggagagtacc aaaatatttt ccaagcatgg cataatcaat
    14341 tcgttgcaag tagcaaagcc gtaaaaatcg gccacgaatt gcgttcggat atccaaatcg
    14401 gctgcatgat tatctatgca acaacttaca gcatcgatgc caatccagtc aatcaggcag
    14461 caacaatgat ccaaaaccaa gaattcaatt ttttctgcac ggatgtccaa gtacgtggag
    14521 aataccctgc atacacagcg cgtacccata aaaaatatgg agtcgatccg gaaaaattag
    14581 agcaaacgga aaaagacttc cagttgctaa aagaatatcc agtggactac atcggattta
    14641 gttattatat gtctacggca atcaatgaga ctgatccaca agcagcaact tcagaaggga
    14701 acttgctggg aggagtaaaa aatccgtttc ttgaggcaag tgaatggggc tggcagatcg
    14761 atccagaagg gctgagaatc gcattgaatg aattatacaa tcgttaccag aaaccattat
    14821 tcattgttga aaacggtctt ggagccattg atcatatgga agaggatggc acgattattg
    14881 atgattatcg gatcgattat ttacgtcgtc atatcgaagc gatggctaat gcagtagcag
    14941 acggtgtaga tttgatgggc tatacgcctt ggggatgtat cgatcttgtc agtgcatcaa
    15001 caggagaaat gagtaaacgt tacggcttta tctatgtgga tctagacgat gaaggaaatg
    15061 ggacactgaa tcgatcaaag aaaaaatctt ttgattggta taaagaagta attgcctcaa
    15121 atggcgtaaa cttgtaatta aagcagctga atgaaaaaga acacacttct cacatgtgat
    15181 ttgtgttctt tttttatttt ttatgtgcta ttcttttagt aatagtaaag caaagggtga
    15241 cgttatgaaa aggacacgtg tactatactt ggaagtcgca gataaaatta aagaagatat
    15301 tttctcagga aaatatccag tcggttcgat gttgccgaca gaaagtgaat tagaagaact
    15361 gtttcaagtc agcaaaatca ctgtccgaaa ggcaatcgaa ctgctggcat cagatgaata
    15421 tgtcgcaaag aaaagtggtc gtgggacgac ggttctaagc aatcgtcctt acaatcgatt
    15481 atccaaagcg gcaagcttta cgcaaatctt ggagaactcc cgatatgaag tccggaaaga
    15541 aacgctggat ttgcaattgg tccatataaa gaaggaagat cctttgtttt cctttttcgg
    15601 tgagcaagct gtttgtttta agcgcttata ttatttgaac aatcagcctt atatttattt
    15661 tgttcattat cttccacaag aaatgatgca attcagcaga gaagaatttg gcaaagaatc
    15721 tttgtatcgt ttgctgacga aaaaaggata tactattgac tcattttctg atgatttctc
    15781 ggcagtattt ttaacagaag aagaacaagg aattctgaaa acagacgcac agatcggcat
    15841 caaacggata cggaaatcgt ctacgcaaat tggggaaatg attgaatatt cggaggcggt
    15901 ctaccatacg gagctgcatc cttaccatat tgattatgaa acataaataa aaaaagaggt
    15961 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gcttctcata
    16021 tttttcgttt ggttaggctt attaccgcag atacacggct gtttctggat taccatttat
    16081 tctggataag ggggtgtggc acgattttca gaaggaatct atgaaccaat cactgtattg
    16141 attttagctt cgatggtaac cccattcggc ttgacgatcg cttatttctt agggaaaatc
    16201 attcgtaaaa atatactcaa tcgtcaagaa atcgatacat tgaagaccgc ttttcctatg
    16261 gggatctgtc agatcacaga gggatgtttc cctatcgtat tgaacgactt ggcacgcaat
    16321 gtcatagcaa cgggagtcgg tgggacagta ggtggcggat taagcatgtt ttggggcgca
    16381 gatagccgta ttccggcatc gggaatgttt gcagtcgcta cgatgaccag accatgggca
    16441 ttcattggtg cattgttagc tggttctttt gtcacaggaa taaccatttt attattgaaa
    16501 aaaccagtag atccgaatgc agaaatcatc caagaagaaa aagaggaaga agacatatct
    16561 tgggatgatt tgacaattag ttaggagagg cagaatggat tacaagaaaa aagtacaata
    16621 gatgtacctt gaggcaggaa tggtatgaaa gaatcagagt tagaaagaat cgaatataca
    16681 gattttggtt taggagcgat cgaaactgtc cagagcatcg gcaccctcct tgaaatgacg
    16741 atgaaggaaa aatgagaacc tttcgttgtc gaaagggtct ggtatacctt atgtcgaagg
    16801 aaaggaaaca aaaaaacttt cctgtactat tccaaaaagg aaagaaagtt attatacagt
    16861 aaaaaaagaa attattcttc atgctagaga tcagtacacg ttcgagccca atatcaaaca
    16921 ctagttccag gcaggcgcaa caggtgcggt tatcactgaa ttttcttcca gcagtgacga
    16981 ttcaagtgat atttttacaa atccagctat ttatcgttaa caaaaagatt tttaggagaa
    17041 gctgatgaac aaaaatgatc aaattttgat cgtttagttc gtcggctttt ttatttagag
    17101 aaacatcatt tatcagtgaa acataacacg agaacaaaat gattttttcg ttaataaaag
    17161 aaacaagtat ttg
//
LOCUS       DAZKNO010000047        17028 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000047 DAZKNO010000000
VERSION     DAZKNO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.047"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..126)
                     /locus_tag="ACG8MY_001191"
     CDS             complement(<1..126)
                     /locus_tag="ACG8MY_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0430401.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="ACG8MY_001192"
     CDS             complement(230..541)
                     /locus_tag="ACG8MY_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0430402.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEEQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="ACG8MY_001193"
     CDS             complement(543..740)
                     /locus_tag="ACG8MY_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0430403.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="ACG8MY_001194"
     CDS             complement(1134..2861)
                     /locus_tag="ACG8MY_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0430404.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="ACG8MY_001195"
     CDS             complement(2854..4047)
                     /locus_tag="ACG8MY_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0430405.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="ACG8MY_001196"
     CDS             complement(4234..4878)
                     /locus_tag="ACG8MY_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0430406.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="ACG8MY_001197"
     CDS             complement(4972..5106)
                     /locus_tag="ACG8MY_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0430407.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="ACG8MY_001198"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="ACG8MY_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0430408.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="ACG8MY_001199"
     CDS             complement(7237..7710)
                     /locus_tag="ACG8MY_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0430409.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="ACG8MY_001200"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="ACG8MY_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0430410.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="ACG8MY_001201"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="ACG8MY_001201"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0430411.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="ACG8MY_001202"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="ACG8MY_001202"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0430412.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="ACG8MY_001203"
     CDS             complement(11757..12896)
                     /locus_tag="ACG8MY_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0430413.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="ACG8MY_001204"
     CDS             complement(13205..13918)
                     /locus_tag="ACG8MY_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0430414.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="ACG8MY_001205"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="ACG8MY_001205"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0430415.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="ACG8MY_001206"
     CDS             15107..16639
                     /locus_tag="ACG8MY_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0430416.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..17009)
                     /locus_tag="ACG8MY_001207"
                     /pseudo
     CDS             complement(16830..17009)
                     /locus_tag="ACG8MY_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         5282 a         3500 c         2916 g         5330 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gttcttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaatacagac ccgtggtgct agttaatt
//
LOCUS       DAZKNO010000048        16790 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000048 DAZKNO010000000
VERSION     DAZKNO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.048"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(168..1673)
                     /locus_tag="ACG8MY_001208"
     CDS             complement(168..1673)
                     /locus_tag="ACG8MY_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0430417.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1670..2356)
                     /locus_tag="ACG8MY_001209"
     CDS             complement(1670..2356)
                     /locus_tag="ACG8MY_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0430418.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2549..3970)
                     /gene="gndA"
                     /locus_tag="ACG8MY_001210"
     CDS             complement(2549..3970)
                     /gene="gndA"
                     /locus_tag="ACG8MY_001210"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0430419.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4140..4319)
                     /gene="rpmF"
                     /locus_tag="ACG8MY_001211"
     CDS             complement(4140..4319)
                     /gene="rpmF"
                     /locus_tag="ACG8MY_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0430420.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4377..4946)
                     /locus_tag="ACG8MY_001212"
     CDS             complement(4377..4946)
                     /locus_tag="ACG8MY_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0430421.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5132..6034
                     /locus_tag="ACG8MY_001213"
     CDS             5132..6034
                     /locus_tag="ACG8MY_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0430422.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6069..7853)
                     /gene="pyk"
                     /locus_tag="ACG8MY_001214"
     CDS             complement(6069..7853)
                     /gene="pyk"
                     /locus_tag="ACG8MY_001214"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0430423.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7927..8889)
                     /gene="pfkA"
                     /locus_tag="ACG8MY_001215"
     CDS             complement(7927..8889)
                     /gene="pfkA"
                     /locus_tag="ACG8MY_001215"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0430424.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9057..12371)
                     /gene="dnaE"
                     /locus_tag="ACG8MY_001216"
     CDS             complement(9057..12371)
                     /gene="dnaE"
                     /locus_tag="ACG8MY_001216"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0430425.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLIKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLIENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKDGSQIPLENLSASLPLT
                     ANGYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            12598..12783
                     /locus_tag="ACG8MY_001217"
     CDS             12598..12783
                     /locus_tag="ACG8MY_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0430426.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12845..14041)
                     /locus_tag="ACG8MY_001218"
     CDS             complement(12845..14041)
                     /locus_tag="ACG8MY_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0430427.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNIFLSLFNFRK
                     VLRSRL"
     gene            complement(14211..15638)
                     /locus_tag="ACG8MY_001219"
     CDS             complement(14211..15638)
                     /locus_tag="ACG8MY_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0430428.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQVLM
                     TIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIATSV
                     GAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATAPAL
                     ILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVIKGK
                     YNEVSVALWIVDILFIINFIILATI"
     gene            15931..16740
                     /locus_tag="ACG8MY_001220"
     CDS             15931..16740
                     /locus_tag="ACG8MY_001220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0430429.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLNNQEDGLAVYLEKLLKL"
BASE COUNT         4919 a         3533 c         2938 g         5400 t
ORIGIN      
        1 tattgttttt tcgttcatct aaaagatact tacatttaat taacggatgt tattctattt
       61 aattgttcgc tagttttttt ataatgacat ccctgaattt ctcacttgct tgtattttgt
      121 accttattct ttgttggata tataaaaaaa caagtaaagc acatgcttta cttgtcttct
      181 gcctttgcga taggaataaa gattctaaaa attgtccctt gccctactac actttctgct
      241 aagatacgtc ctttatagtt ttcaaccagt tgtttagcga tagacaaccc cagaccattt
      301 ccacccttcg ttctagctct tgctttatcc actcgataaa aacgattgaa gatcttatta
      361 aggtcttctt tgggtattcc ttcgccaaag tcctgaatcg cgatctcaaa ttcattcaga
      421 gtggaagaaa tagagatatg gacttctttt cgtgtagttg aatactttac tgcgttatcc
      481 aatatgataa tgattaattg ttcaaagtga ttacgataaa tttgcagggt cacttctttt
      541 gtcaaatcat catctaaagt gatcacatag tctggatata atatcttgaa attattataa
      601 acttgatagg tgacttcttt tcctttagtt gtttcattcg cataatgaac atctacttgt
      661 tctgctcttg ataagtctag catctcttga actaagcttt tcatacggct gatttcttgc
      721 atgctggcgc ttaatgattc ttctaatatc tcaggatcat ctttccccca acgatttagc
      781 agactaagat gcccttcaat aattgcaaca ggtgtcctta actcatgaga tacgtcttcc
      841 acgaattgct cttgttgttc aatatacaaa cgcatacggt caagcatctc attgaatatt
      901 tcagctaaat ctgccaattc atcatttgtg tcgatttcag gcatgtggat atctgattgc
      961 ggatctttac gtatagtatc cattgtatct ctgagtacct tgagtggttt taagaaataa
     1021 gaagataaaa agaaacctaa aatactactt aagatcaaag aaacaacttc taaaacaata
     1081 agagtcaata atagcttatt tcgaatatca tagaaagaag ataattcata gaagctttgc
     1141 gcatagccga tcttttctct tgtaccttta gaatatatag gctggataga taaaaagcca
     1201 gtttttcctt ctagtgtaac gatcgttgga actttacgtg tagtctgaat caggtttcga
     1261 ctgttttcat gggttttaaa aatcaagttc tcgtccaaat cataaacata aagatccatt
     1321 gatggctgcc ctagctcagc tataaaggaa tcgatcttca ttaagtttcc ttcgaccagt
     1381 gaagtatgat ctacgacctc atctccgtca tttgtcgctt tcgtcagatt acgatacgta
     1441 tttactaatg tcagttcttc atccgagtta gccaaacgag aagctacttc tgaaatcgta
     1501 cgctctacat tgtttctttc ttttgccaca atcagattga tagatgattt ataggtaatg
     1561 acagcgaaaa ttgtaaacac aacgaatatg aagaaagaac tcgcaaaggc ccacttgatt
     1621 gtcaaggagg gccctttcag ttctcttttt tccgattttt tcggctttat cacgagcgca
     1681 tcacataacc tgttccgcgt acagtttgga tatagctttc ctccccggga acgtcaattt
     1741 tgttgcgaag ataacgaata tatacgtcca ctacgtttgt ttcaacttct gtttcgtatc
     1801 cccacacttt atttagtaaa acgtcacgtg ctaataccac gttcacgttt tccattaaag
     1861 taagcagcaa ctcgtattct ctttttgtta gttcaattac ttcatttccg cgacgtacga
     1921 cacggttttc tttttcgata gtcaaatcgc gataagtaat agtagtttgt ttagccacat
     1981 ttttatcgcc ttcaatatcg attcgacgca acaatgcacg aagacgtgct aataattctt
     2041 cgatagcaaa tggtttgacg atgtagtcat ctgctccgtg gtcaagtcca gatacacgat
     2101 cgatcacaga atctcttgca gtcatcataa tgattggtgt atttttgact tggcgtacac
     2161 gtctgcagac ttctaaaccg ttcaattctg ggagcatcag atccaacaat atggcatccc
     2221 actcattatt taatgccgca tctaaaccag ttcggccgtt gtagtggact tctgcgttat
     2281 acccttcatg cttcaactct agctcgacaa atcgtgccaa gttcttttca tcttcaatga
     2341 ttagaatatt gctcatttat tattttatcc tttctattct actggctttt taaaaactct
     2401 atcattatac cgataatatg cttaaaaatc tatagtcatt ttatcatgga tagcaatttt
     2461 aaaaaagagc tgaagtctac tcataacaat cgacttcagc tctttcgtcc tctttttatt
     2521 ttctcatttc ctaacagccg aaagactatt attcttcatg gtaccatgag taatggaaga
     2581 ttccttcttt gtcgacacgc tcataggtat gtgcgccaaa gtaatcgcgt tgggcttgga
     2641 ttatatttgc aggcagacgt tctgctctgt atgaatcgaa gtaagcgatg gctgatgaga
     2701 aagtcggtac aggtacgcca gcttggacag ccaaggcaac gacgtctctg actgattgct
     2761 gatatttttt cgtgatttca acgaagtaat catctagcaa taaattttcg atctcaggat
     2821 ttttgtcata ggcatccgta attttttgca agaatcgagc tcggatgata catccagcgc
     2881 gccagatttt tgcgatttca ccaaatggca agtcccaatc aaattctttt gaagcgacac
     2941 gtaattgagc aaatccttgg gcataactca tgattttact gaaatacaac gcttctcgga
     3001 ttttttcgac caattctttt ttgtctcccg tgaactcaaa atcgttcgta cgagacaaaa
     3061 tcttgcttgc ttgtacgcgt tcttctttat aagcagaaat atagcgggca aatacagatt
     3121 cagtgatcaa tggaagtggc acacctaagt caagcgcgct ttggcttgtc catttacctg
     3181 ttcctttatt cccagctgca tcaaggatca catctactac tggtttacct gttccttcat
     3241 cgtctttacg tgtcagaatg tcagcagtga tttctatcaa atagctgtct aattcgcctt
     3301 gattccattc tttaaagata tcagccattt cttctactga aaggtttaaa atattcttca
     3361 ttaagtcata agattcagca atcagttgca tatctccata ctcaataccg ttgtgtacca
     3421 ttttcacata atgtccggct ccatttgggc caatatatgt gacacatggt gctccgtctt
     3481 ctgcttttgc agaaatttgc tctaagattg gtgcaactag cgcataggct tctttttgtc
     3541 caccaggcat gattgaaggt ccttttaaag ctccttcttc accaccggaa acgcctgtac
     3601 cgataaagtt gatgccagag ttggctaact cttcattacg acgcatcgta tctttaaaga
     3661 atgtatttcc tccgtcaatc aaaatatcgc ctttatcaag gtgaggaagt aactcttgga
     3721 ttgttgcatc tgttgccggt cctgctttga ccattagcaa gatccgacgt ggtttctcga
     3781 tagattctac aaattcttcg attgtatatg ttgctttgaa atttttatct gggtgttctt
     3841 ctactacagc ggttgtttta gcccctgtac gattgaataa agccactgaa tagccgcggc
     3901 tttctatgtt caaggctaga tttttaccca taacggccat accgacaacg ccgatttgtt
     3961 gttttgacat atgaactcct cctacatacg ggatttctcc ctaatctctt acattatagc
     4021 ttaacacggc tacattgcaa actaaagaac aacatatttt ttatttaccg actacaaaaa
     4081 aataaacctc aaggctccat ggttagcctt gaggttttac attttctaat taaaatgtat
     4141 taagcttctt ttgacattac gtccttacca tcatagtaac cacaatttgc acatacgtga
     4201 tggctttttt tcatttcacc acagtttgga cattcgttta accctttgat tgtcaatttg
     4261 tagtgtgtac gacgttttgc ttttttagct tttgatgttc ttctagctgg tactgccatt
     4321 ttgctacacc tccttgtaaa gtcttgtatc taaattagac acaatatatt ccaatcttac
     4381 ttgttatcgt cgtcttttga tttagaatca aaaagttcag atagttttgc aagacgagga
     4441 tcaattttat tctcggcttc ttccattttt ctgcgctggt attcttcttc ggaaacaact
     4501 tcccaatcat tgccttttgg aagttctgtt gctgcttcct cttcttttgt caatacccgc
     4561 attggaatcg ccaataagat gttgtcttca atcgactctg ctaaattcaa taatggacct
     4621 tcaattagca aaatctcatc tgcagacatt tgttcatctc tactttggaa ttgttcttct
     4681 gtcatgaata cttcgtctac agaaaaatcc atgggcagtt caacaggttc tagtgaacgt
     4741 gttgaaggaa ctgtcaaagt cgtctgcaca gtgtaatgaa cgatatagcc tttcttatca
     4801 actgagacca aaccttcgac ttccactggc gcaacatcaa gtattaaatt gtctcgcttc
     4861 atgagagcgg ctttcaagtc gagcgtctca tgaaaagtta acggtgtctc ctggtatttt
     4921 ctcaattcca ataaagacca tttcattttt catcactcct aagcaacaaa agcaattata
     4981 cagggcaaaa agcatgttgt caatgaaatt ttcttgacac cttgcagttc ataaaaaaac
     5041 agaaaaatct ttattagaaa gcctctgttt gctatcatcc tcagaatcat ttacaataat
     5101 cattattcca ttaaaggagc ggatgatctt catgaacacg aaagatatcg aatactatgt
     5161 ccaaatcgtc aaacaaaaaa attttacgaa agtggctaat tatttccgtg tgagccaacc
     5221 tactattaca tatgcattac aacgtcttga aaaagagttt gacaccgtac ttattacaag
     5281 agatcgatcg catcacgaat taatcgtcac gccttctgga aagcagctgt ttctccatgc
     5341 acaaaccatt ctccaagaga tttctactgc aagagctgaa atcactcagt tgcaaaagaa
     5401 aaagcttcgt tttggcatgc ctcctattat cggcaatgaa tactttccta aactgtcaag
     5461 ttactttatc aagcatgaat tgatgaatca aattgagatt accgatggag gctctcggga
     5521 cttatacggg atgatccgac aagggaggat cgatttagct gtattaggct caacacagcc
     5581 aatcgtggat caatatttag aaacagagtt acttttggac aaacgtttca tgatcgtcgt
     5641 gccacctaca catcctcttt ccaagcagga tactgtctct ttcaaagata tagcagatga
     5701 acagtttgta ctattgaacg aacattatgt ccaccccgca gcattcacta agctttctaa
     5761 acaaacacat actcatccaa aagttgttta tcaaagcaat gacttgacga ttttaaagag
     5821 tatggtccga gaaaatgtgg gtatcggttt tttggcagag attgctgtcc atcctgaaga
     5881 tcatttagtt tgcctccctc tttcagatga agttcagcct cgcttcttga tttcattggc
     5941 aaaacgaaca cagcagatcg tcacgcctct tcaagaacaa gtagtccaag ttatccatga
     6001 attcgttaca gcacaaaaaa acagctcttc gtaaaagaag agctgttttt attttttatt
     6061 aagctaaatt agatagctgt tgtttcgcca cggtaaacga tgccgcgacg tgaatcaacg
     6121 gtaatcaatt caccttctgt aattgttgtt gttgcgcctt tagcaccaac gattacagga
     6181 atatcttgtg caattcctac aactgcagcg tgagaagtca atccgccttc ttcaacgata
     6241 acagcagctg ctttttcgat tgcaggcata tattctttgt cagttgatgg tacaactaga
     6301 accattccgt cttttgcttt ttttacagct tcttcagcag atgaagcaat aactgcattt
     6361 gcaacgacag atttttgtcc aacgccttga gcttctaata atttagaacc aatcatttga
     6421 attttcatca cgttagttgt tccgcgttca ccaactggta caccagcagt gatcaagatt
     6481 aaatcgcctt ctgatgcgaa acctaattca acagcttttt tagctgctag ttcaaacatt
     6541 tcatcagttg ttgatggttt ttcagcaact gttgggtaaa cgccccagtt taacatcaaa
     6601 ccacgttttg tgcgttcgtc aaaagtaaca gccaagatat ctgcatctgg acgatatttt
     6661 gaaatcattt ttgcagtgta tcctgattca gttgcagcaa cgattgtttt cacgcctaga
     6721 ttttttgcag cacgagctac agataaaccg attgtttcag ttacatctgt tttatcgaat
     6781 tcgttcaatt ggaatgtacc taattctgat aatgcatttt cagcttcttg gtcgatacgt
     6841 gccattgttg aaacagcttc aactgggtag tccccgtttg cagattcacc agatagcatt
     6901 gttgcatctg tgccatcaaa tacagcgtta gcaacgtcag aagcttccgc acgtgttgga
     6961 cgtgggtttt cttgcattga ttctaacatt tgtgtagcag tgataactgg gataccagca
     7021 gcattacatt ttttgataat gcgtttttga accattggta ctagttcagc tgggatttca
     7081 acacccatat ctccgcgggc aatcataaca ccgtcagaaa ctttgatgat ttcgtcaatg
     7141 ttgtcgatac cttcttgaga ttcgatttta gggaagattt gaacatgagt catgtctttt
     7201 tcttctaaga tttcgcggat atctagaacg tcttgtgctt tacgtacaaa acttgcagcg
     7261 ataaagtcga tatcgttgtc taaaccaaaa cgaatatcgt cagcatcttt tggagtaata
     7321 cctggcaaac taatagaaac gcctggtgca tttacccctt tacgtgatcc tagcatacct
     7381 gcatttttaa cttctacgac taattcacgg tttgcttcgt ctttttcaat gatttgcata
     7441 tcgatcaaac catcatcaaa taaaatgtgt ccgccaacgt gaacatcgtc aaataatcct
     7501 gggtaagtaa ccgcgatttt ttcttttgaa cctacatgtt caggatccat agagatgcgt
     7561 gtttgatctc ctacttcaaa tttgatgtat cctgcacgac caaaatcagc ttctgttgtg
     7621 ccttgaacag ttgtacggat ttcagctcct tttgtatcta aaagaatacc gacatctttt
     7681 cctgtctttt taactgcttc acggaccata gtcatacgtg ataattgttc ttcatggtca
     7741 ccatgagaaa aattaaagcg gaagacattt gccccagatt caatcaattg agaaataatt
     7801 tctactgagt tacttgctgg tcctaatgta ctaacgattt tggttttttt catttaaaaa
     7861 actctcccat tcaattttgt ttatttaaag ctgtacacag cttaaatagc ttggtttctt
     7921 tcactcttaa aatgaaagtg aattatttag atcatatagt gataagtctg gtttatgttt
     7981 gttgttttca agtgtatcaa tgatgtcaga cgcaacgatt tcgttgtcat ggataccgac
     8041 acataatcca ccttttcctt cttgaagcaa ttcgacagcg taagcaccga atttacttgc
     8101 caacacgcgg tcgcgtgcgc ttggtgctcc accacggaca acgtgtccca agatagatac
     8161 acgtgtatga tagtcaccaa attctgaaag tttctcagcg aattcatttc cgcccattac
     8221 accttcagcc aagatgatca agcaatgttt cttcccgcga tcgcggcctt cttggatttt
     8281 tttagcaact gaagccatat caaaatcatg ttctgggatg atgatttcat ccgctccgcc
     8341 agccacacct gaccataaag cgatgtctcc tgcattgcgt cccattactt cgataacgaa
     8401 tgtacgaaca tgagaagtcg ctgtgtcacg aatacggtca attgattcta gtactgtatt
     8461 gattgctgta tcaaaaccga ttgtgaaatc agtcccagga atatcattat cgattgttcc
     8521 aggaatacct actgctggga aaccgcgttt tgtcaaagcc attgcgccat gataagagcc
     8581 gtctcctccg ataaccacta gaccttcgat tccgaatttt ttcaattgtt cgattccttt
     8641 taattgacct tcttcagttg caaattctgg ataacgtgca gaatacaaga atgtaccacc
     8701 acgttggatc ttgtcaccta catctgccac atcaagacga cgaatatctc cagcaactaa
     8761 accggcaaaa ccataattga tcccgtatac ttccagtcct tcgtaaatac ctttacgaac
     8821 aacggcacga acagctgcat tcattccagg tgcgtctccg ccactggtca aaattccgat
     8881 gcgtttcata ataaacattc acctcattaa aatactttat cctaagtatg acttaggttt
     8941 acaaccattt ttgcgttaac cacgactaac attctaacat tatttttctc acttgtcttg
     9001 atttacggat aaaaaaatga aaaaaaatga aagcaagtag tattttttac cgtaagttat
     9061 ttgaaaacca cattatcttc gccaagcaga taaataagtt gcccctttat actcttgctg
     9121 tcatccaccc aataagcatc tgacagaact acttttcgtt gcgttttttc gtgatacata
     9181 actactggta tatatcctgg gtgtttctga aaaatcactt gtaattgttt cattatctcg
     9241 ggatcttccc gttcttctgt aatacgcaaa tagcaagttt gtgaaggata ctgggcttct
     9301 aactggttag gatcagcaaa gctttctcca attagttgca gttcctgatt atatttgctg
     9361 atttcgctcc gcccttgaac aaagatcact cgatcaacgg caaacaaatt acgatattta
     9421 cgataaagag ttggaaaaac tgtgacagaa atttctccac tactgtcggt tccttcaaca
     9481 aaggccatca gttctccttt ttttgtccga atttctctaa cttccttgat catcactaat
     9541 aaattgactg gttgattagg gacgatatct gtaatcagct gaacttgttt agctaaacga
     9601 agacgatcaa acccttctgc aggatgacct gacaaataaa tacctaagta ctcctcttcc
     9661 agcttcaagc gttcttctag tgtatagtca gcgacttctt cttcttttaa tgccatgatt
     9721 cccagcaagt caagacttcc accactgtaa acgatatttt ggatttttcc ttcgagatca
     9781 agcatcaatt gtttcctgtt aggagataat tcatcaaaaa ctcctactgc tgtcagtgat
     9841 tgcagtaact ctaatttcag ccaacgctta tcgatccgta tgaggaattg atccactgac
     9901 tggaacgggc cattttgttt tctttcgttt aatatatctt ctacaaaatc acgccgaata
     9961 ccttttatcg ctgtcagtcc gaatctgatt tcttccttat tgaccaactc aaaactggaa
    10021 ttactttgat taatggacgg cgaggaaagt ttgagtttat ttttacgtgc ttctgcaata
    10081 tattctttca attttgatgt gttatgccga acagaattca tcaggctggt aaagaaagca
    10141 cctggatgat gggcttttag atacgccatt tggaatccca cgaatgaata agcaaatgca
    10201 tgtgaccgat tgaatccata gtttgcaaat ttttcgatat attcatatac ggtcgctgct
    10261 gttttttccg aatatccctg ttgtaatgct cctgatacaa aatgcgactt ttcttcatct
    10321 aatacgtctt ttttcttttt actcactgcc cgtcgcagaa tatctgactg ccctaatgta
    10381 aatccagcca tagtcgaggc gatctgcatg atttgttcct gatagacgat cactccatat
    10441 gtgtttttca aaatcgggat cagtgaatca tctgggtagc gaatggcttc ttttcctttc
    10501 ttcctggcaa tgaaggtatc tatattttgc atagggcctg gtcgatacaa tgcattgact
    10561 gcggcaatat cttcgatgtt ttctggatct aatcttcgta aaacgttccg gataccagca
    10621 gattcaaatt gaaatactcc tgaggtttcg cctcgttgaa acaagagcag cgtttttgca
    10681 tcatttagcg ggatctcttt taatcggatc tcataccctt ctactcgctt aacagctttc
    10741 aaagtaaaat caataatcga taagttgcgt agtcctaaaa agtccatttt caaaagaccg
    10801 attgcttcta cctcattcat cgtatactgg gtcagaaaag cttcatcaga accgcgttgg
    10861 agcggaacta gttcaagtag attcttatcg ctgataacta cacctgctgc gtgagtcgaa
    10921 acatgtcttg gcaatccttc taaaatcact gcagttttga ataaaagttg gtttttttcg
    10981 cttgcctgaa ctaattctac taattgatgc gaagttttat aagcttgggt caatgtgatt
    11041 ttcaattgat tgggtattgc ttttgaccag cggtttgctt cactttgcga taaaccaaac
    11101 actctagctg catctctaag aaccattttt gctgccattg tgccaaatgt tgcgatttgt
    11161 gccatatgat attgtccata cttttctcga acataattca atacttcttc acgtcgattg
    11221 tcaggaatat ccaaatcgat atctggcatc gtaaagcgtt cggggtttaa aaagcgttcg
    11281 aacagcaaat catattctaa tggatccaca tctgtgattt ctaacacata ggctactaat
    11341 gaacccgcag cagaaccacg acctgcaccg gtaacgattt tgtttctatg agcaaaagcc
    11401 attacatccc atacgatcaa gaaataatca tcgaatccca tttgatggat gacagataac
    11461 tcgtagtcca aacgttcttg gtattccttt gtatagtcag tgattcggcg ttccacttgc
    11521 gaatagcata actctcttaa atattctcca gcttgttttc cattaggaac tggataatgc
    11581 ggaagtaatt tctgatggaa ctggaattca aatgaaatcg attcagctaa acgctgggcg
    11641 cctattacag cttgggaaag acccgctttt tcgaatcgtt tgatcatttc ttgttctgaa
    11701 agaagatagc catttgctgt tagtggtaaa gatgcagata aattttctaa aggtatttgg
    11761 cttccatctt tgatatgttt catcacttca agtgcaaaaa gttcttcttg ttgtaaacta
    11821 gcgatgggat gtaatgcaca aagcgaataa ttttctttta tcacatactg ataccattca
    11881 ggcaattgat caggcgtgtt ttctatcaag ccgatataaa aatttctagt aaaaacattt
    11941 tcaagtaaaa actgccaatg accgaaaaag ttatcctctt tattgaaagc ctcagaaaca
    12001 gaatgatttg ccggaacaac aaacgctaga tggtcggttt ctgtcatttc tgcaagttcc
    12061 aatttttctt ggatcatttt tcggctggaa agttttatca gctgctgata acctatttca
    12121 tcttttgcgt acgcaagcaa gccataattt ttttcttcat aagtataact aagatgtaat
    12181 ccaatgattg gctgtactcc ttcttctaca caaatacgat agaattcagg tgcaccgtat
    12241 aagacatctt tatccgttat ggctaatgaa gtgtagcctt gtttttttgc ttcttgtaca
    12301 tattctcgaa tacgtatcgt actttgtaaa agagaataag aagtaattgt ataaagttgt
    12361 gggaaaaaca tcttgcattc cgcctttttt ttcgttaaaa ttcactagcg ttaaaaggtt
    12421 tgatcctttt actttcatta agatagcagg cgctgaagtt gattattcca cttcatttgc
    12481 ttgtaatctg taggaatgag ttctttcgca agaactcaaa ttttattgag atttggtctc
    12541 gtttctttac attcaaagaa aatctggtaa aattaagata gaaaagaggt gttctttatg
    12601 cgatatatcg ttacattatt ctgggctgtc gttctaggtc aagtagtcgg ttatattggt
    12661 gctgcattaa caagcggaac atatgatttt actttgacaa caatcatttc attcattgcg
    12721 ggtgtcatta tccttttgat tggtgcagtt gcgccaagaa aagaaacctc tgcccattcg
    12781 taaaaaaaca gccaagagct tgagatatct caagctcttt tttattttct gctcctcacc
    12841 accattataa tctgctgcga agaactttac gaaagttgaa taaagaaaga aaaatatttg
    12901 acagaacaaa caaacttgtc acaataaaaa cagcactgta attataagca tgcgcgactg
    12961 tcgatcctag catgggacct aaaacttgtc caaagttgct aaacatctga ttgtagctat
    13021 agattcggct gactccttct tgaggcgtaa tcttactgat cagtgtattg acagaaggca
    13081 tcaaagcgcc agttgaaaag cctaaaataa agcgaagtat acctaattgg agcggtgtag
    13141 tgacgaatgc cataggtaaa taacagcaaa atgataaaat gagtcctcca attagaactt
    13201 tttgattccc aatcttatcc cctagttttc ctagtttcgg tgaagaaaaa acagcggaaa
    13261 caccagctat cgaaacaatc aagccgctga caaaaaggat attttctgta cttccgctca
    13321 attcacgaat atataaggtc aaaatagggc tgatcgtagt gattcctatc tgtaaaatca
    13381 aggtcgtaat gaagagaccg atcaaaatag agagatgatc catttttgaa aaaatttctt
    13441 tggtgcttat cagatctttt ttctcgatcg gatggaaatc ttctttgact aagaaaatgg
    13501 tcaataccgt tgtgatcatc aacaaagccc ctgtgatcaa gaatacattt tccataccaa
    13561 accactgagc cagtgcaccg cccattgaag gtccgatcag atttccagca atcgctccgg
    13621 tagacaatgt tcctaacgcc catccgctct tttctctagg tgcctgtgaa gcaatcatcg
    13681 ctgtggcatt tggaatatat ccagacaaaa ttccattcca aaaccgcatg atcaaaagcc
    13741 aatacgcgtt cggtacaaaa gccaaagatc ccattgttaa tgtcatccct gctgctgcac
    13801 ggatcatcat gagttttcgt ccttttcgat cagctagatt cccccagatt ggcgcaacca
    13861 cggcagatgc aaaagcagtc acagagattg ctagtcccga aaacaattcg atttgatctt
    13921 ttggtgttcc tagttgctca acataaactg gtatgaatgg cataactaaa ctaatacttg
    13981 caccagtgaa aaaacagcca atccacgcaa tcactaaatt ttttctccaa ttaatctcca
    14041 tctatcatcc ctctttcgtc tcttttatca tacctatttt cgaataaaca agcaacgatc
    14101 tcgccagtaa aaagagaaaa atcccgcaaa ggtcatttta tatgtaacgt tctttttatg
    14161 taaaacaaaa agagatcaga acaaatactg ttccaatccc ttctctttag ttaaattgtt
    14221 gctagaatga tgaagttgat aatgaataaa atatcaacga tccacaaagc aacagaaact
    14281 tcattgtatt ttcctttgat tactttcaca attgtataga aaataaatcc tgctgcaatc
    14341 ccataggaaa tgctatagca caatcccata aagatcgatg caaagaaggc cggtaaagct
    14401 tcttccatat ctagccattt gatgtctgca aaagaagcca tcatcattac tccaactaaa
    14461 atcaatgctg gcgcagttgc ttgatttggt acaatggcga tcagtggaga gaataaacta
    14521 cttaatgcga atagcactgc cactactact gaagtcaaac cagtacgtcc accggccccg
    14581 atcccagctg cagattcgac ataagttgtt gtattcgatg taccaaagat agcccccacg
    14641 gaagtagcga tcgcatcggc aaatagtgct ttatccattt ttgttttgaa cccacgacca
    14701 tcttccaacg cgatctcgtc ttcttttgag aaaatgcctg tgcggcgtcc tgtaccgata
    14761 aatgttccga tcgtatcgaa agtatcagat aaactgaatg cgatgatcgt catcaatact
    14821 tgaggaatct tggaagaatc actgaataaa gattgcatgc cttcagaacc aaatgctgca
    14881 ccgaaagttg ttcctaattc tttaaatgaa ttacctaacg aattcgtttg ccaacttaaa
    14941 tcaacaacac ccataaaaat accaaggact gtggtcgcta cgataccgat aagaatagcc
    15001 ccacggacat tttttacaac taaaatggct gtcagtacca aaccaatcac agccaataag
    15061 ataggagcgt tgtcaaagtt ggctaacgca gggacgattc caccatttat cgtaacattt
    15121 gtcgcttttc cgccttcaac gacagagctc gaaatagctg actggtctgc tgaaaaagat
    15181 aagaatcctg catttttgat tccgacatac gcaacgaaaa taccgatccc gccaccaatc
    15241 gcatgttgaa gactttcggg aatcgcccga ataatcattt ttcgaatatt tgtgacagta
    15301 ataaaaatat tgattaatcc gcaaataaac accattgcca aggcttgttg ccaagaataa
    15361 ccaagcccaa aaacaaccgt aaaagtgaaa aatgcgttca atcccatacc aggtgcctga
    15421 gcatatggta catttgcgaa caatcccatt accagcgtac caataatcga tgcaatgatt
    15481 gtcgctagaa atactgcttg gaagggcata cctgatgctg acaaaatcgt cggatttacg
    15541 aataagatat aactcatggc aaagaatgtc gttactcccg ccattatttc agttgataca
    15601 tttgtattgt ttttctttaa accgaaaaac ttttccataa aaagttccac tccctgaatt
    15661 tttttctgat ggaaaactat aataataaaa aataagtaaa atagggcaaa tccatcgaac
    15721 aatttcaaat tatatagacg tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga
    15781 aaataatact taacgttcgt gtttagctta tgtttagcta tttatatagc cataactgta
    15841 gtttttgtta ggaatagctg attgctcttg ttaattacct aaaatatgct agactaaaac
    15901 aaatgctagt tgaggaagga aggaccctta ttgaataaaa aattaattgc catcgatctg
    15961 gacggaacta ccctcaatca agattcgatg attacatcta aaacaaaaga gactctgaaa
    16021 aaagcaatca atgccggtca tcatgtcagt attgctacag gacgcccatt ccgaatgagc
    16081 catcagtttt accaacaatt agaattaaca acaccgatgg ttaattttaa cggcgcatta
    16141 gtacacatcc ctaatcagca ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc
    16201 gcttttgaaa tcttatcgca aaaaaagcag ctaaatttag attttatcgc tgctgaaaat
    16261 cgtgatactt tttttattga cagttttgat ttctttgatg aaaaaatttt tgcttcttct
    16321 cgtccaggag agaagaattt gctttctcct aaaaatttga cgacaaatcc tacatctttg
    16381 cttgtccgaa cagacaaacg ttttgcagga gctgtatctg ctgaattaac acgtcaattt
    16441 ggcagttatg tcgatgttcg gacatggggt ggacctactg ccatcttaga gattgtttca
    16501 aaaggaatcc aaaaagcaaa aggcgtgcaa gagattgcta actacctgtc tatcgatcaa
    16561 aaagacgtca ttgcctttgg tgacgagcat aatgatttgg aattgttgga ttatgcaggt
    16621 tggggagtag ctatggctaa cggtactgat caactcaaag gcattgcaaa tgatgtcact
    16681 cctttgaaca atcaggaaga cggtttagct gtttatctgg aaaaactact taaactataa
    16741 tagagaatta gattccacaa aaaagaggtt gtgacaattt ttgtcacaac
//
LOCUS       DAZKNO010000049        16707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000049 DAZKNO010000000
VERSION     DAZKNO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.049"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1422
                     /locus_tag="ACG8MY_001221"
     CDS             82..1422
                     /locus_tag="ACG8MY_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0430430.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1640..2083
                     /locus_tag="ACG8MY_001222"
     CDS             1640..2083
                     /locus_tag="ACG8MY_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0430431.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2348..3766)
                     /gene="nhaC"
                     /locus_tag="ACG8MY_001223"
     CDS             complement(2348..3766)
                     /gene="nhaC"
                     /locus_tag="ACG8MY_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0430432.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4169..5392)
                     /locus_tag="ACG8MY_001224"
     CDS             complement(4169..5392)
                     /locus_tag="ACG8MY_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0430433.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVEQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5423..6262)
                     /locus_tag="ACG8MY_001225"
     CDS             complement(5423..6262)
                     /locus_tag="ACG8MY_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0430434.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETTVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDEKNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6279..6959)
                     /locus_tag="ACG8MY_001226"
     CDS             complement(6279..6959)
                     /locus_tag="ACG8MY_001226"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0430435.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(6961..8034)
                     /locus_tag="ACG8MY_001227"
     CDS             complement(6961..8034)
                     /locus_tag="ACG8MY_001227"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430436.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IIEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKINILQKNELDSRIKVIEGGR"
     misc_binding    complement(8090..8328)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8370..8603)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8680..9351)
                     /locus_tag="ACG8MY_001228"
     CDS             complement(8680..9351)
                     /locus_tag="ACG8MY_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0430437.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYFDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(9438..12086)
                     /gene="mgtA"
                     /locus_tag="ACG8MY_001229"
     CDS             complement(9438..12086)
                     /gene="mgtA"
                     /locus_tag="ACG8MY_001229"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0430438.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLTFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASMFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGKFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNVTFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIHRFQELL"
     regulatory      complement(12428..12602)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            12775..13212
                     /gene="ndk"
                     /locus_tag="ACG8MY_001230"
     CDS             12775..13212
                     /gene="ndk"
                     /locus_tag="ACG8MY_001230"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0430439.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAVDAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13234..14100)
                     /locus_tag="ACG8MY_001231"
     CDS             complement(13234..14100)
                     /locus_tag="ACG8MY_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0430440.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICSKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLSLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEKKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14260..16353)
                     /locus_tag="ACG8MY_001232"
     CDS             complement(14260..16353)
                     /locus_tag="ACG8MY_001232"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0430441.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWMKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVLYWLDIATFIGILVWLTIKKIPIKSQPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFSESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSAIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWNEFDN
                     AQMQRVPLMIHIPGYTKGSVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSPQHD
                     QVVAFRNGNFVTPKYTILGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKSALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNIAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKDNQ"
BASE COUNT         4823 a         3453 c         2936 g         5495 t
ORIGIN      
        1 gttatcatta gtcattgtta aatattgcta tttattctta atataaattt tgattgtgaa
       61 tttaaaagaa agaggtcatt tatggaagat aaaaaattga aaaaacaact tagctccaga
      121 catatcacca tgttagcttt aggtggagca atcggtgcag gtctatttaa aggaagtggc
      181 gaagcgattg ggatcgccgg cccttctgta ttgattgctt ttttgattgg cgggctgatt
      241 ttatatatcg tgatgaaagg tctgggaaaa atcgttttaa gcggtggcga cacgcatcac
      301 ggattatctg gtttgatacg cccttatcta ggtgctcatt ctgctgattt tactgattgg
      361 gtctactggt ctatgtggat tatcaatatc atcgctgaag ctgttgcagc tgcttccttt
      421 ttgcagttgt ggttcccgca cattccagcc tgggtatttg tttttatgtt ggctgtttta
      481 actacgatca tcaatcttta ttcggtacgg ttattcgcag aaacagaata ttggctggct
      541 tttgctaaaa tcagtgtcat tatcttacta atcatttttg ctacttatct tgtcggtcag
      601 caaatgttgg gcacaggcgt tttccctacc cttcagcaat tgactgacca tggtgggttt
      661 actcctcatg gtatgaaggg aatcatcagt tctttgcttg tagttatcta ttcttacggc
      721 ggttctgaac tgattgctat cacggtaagc gaagcagatg atcctaaacg tgcgattcct
      781 aaagccatca aaggtgtcat gggaagaatc atttcctttt acatcattcc tttgtttttg
      841 cttttgattc tttttccttg gaatacttta gcaggcacga atgtcagtcc ttttgtgatg
      901 gtatttgaaa aaatgaatat cccatttgca gcggatatcg tcaattttgt tattgtcctt
      961 gctttgttct catctatcaa ttcgggtgtg tatgcatctt caagaatttt gttcttccgt
     1021 ttgaaggatc gaaaaggttc ctctagaaaa ctagctgttt taaacaagca tcaagtaccg
     1081 caacgtgctg ttttattttg taccagtgtt ctttatcttg gtgtagctct ttcttatttt
     1141 gtcggtgaca aattatttgg ctatctggca ggttcccttt cgtatacagt attattgatc
     1201 tggatcatta tcagtgcggc atcctttgta ctggcactaa gaaaaggaac attatgggat
     1261 aaaggaatca gcttttttgc attagctgtc ttaggattga ttttccttgg tatcctgttc
     1321 acgaactcca ttggggtaac tgtattgact ggcctgcttt acctgttcat ttatttcagt
     1381 tatcaaaaaa agaatgatgc gttcgtattg acgaatgaat gaaatcgata gaaaatttta
     1441 ttttatttat gtggaataga tggaactatt gaagaaagga gttgtgacaa aagtcttgtt
     1501 gcaactcctt tttccgaata aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc
     1561 ctctgtgcaa aataattgat tgttctccat gctcatgaca tgatagaaga aaggaatgaa
     1621 aaaaaggagt ttgttttaaa tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc
     1681 aacggtagtt atgtttggta tgatgtactt gaatgtctat gcgctcgatc atatcttctt
     1741 cagtcgtaca cgagtattca tggcgttgat gatgggtgca gtcatggcag tcatcatgct
     1801 gttgttcatg tggaaaatgt atgaaaacaa aaagataaat gttatgatct tagccgctag
     1861 tcttcttgtt tttgccggtt ccttgtttat ggtacgaagc caaactactg tcaacgacac
     1921 agcttggatg aaggccatga ttcctcatca ttctatcgct atcctgacaa gtaaacgagc
     1981 tgatttaaaa gatcctgaag taaaaaaatt agcggaagaa atcatccagg cacaagaaga
     2041 agagatcgac aaaatgaaaa aaatgatcaa agaaatggaa tgaccattat ccgtccaaat
     2101 aaaaaaagag gatgttccag aagtgatcag cttcaagaaa taagaagaag ctacttctgg
     2161 aacaccgtga ataaggggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa
     2221 acaaaaatag cttctcctat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt
     2281 tgaacaccgt ttattcgtaa aagggattgt gacaaaaaaa gtcttgtcac aatccctttt
     2341 tatgttttta ttgttctaat tgcgctgtct tgcttactgc ttcttgcggt ttcacaaatt
     2401 tttttgagat tccccaacct gtgaaggcac tgaccatcga aaacacagga gaaagcaaac
     2461 tgaaaaatac gaatggcaag taagtgagtg taggaatacc aaatgtactt gctacgaaac
     2521 ttccggcaat tccccaaggc actaagtagt tgattactgt tccaccatct tctagcgttc
     2581 tacctaatac agttggatca aatcctgctt tttcataagt ttctttaaat gcattaccag
     2641 gcagaataac agataggaat tgttcaccaa taaaaatatt tactccgata cttgtcatca
     2701 atgttgtaaa aatcaatttt ggtgtgttcg tcatctttct tgatactcgt tccatcacgg
     2761 ttgagatcaa gccaaattcg atcaacaatc cacctaatga caaagttaga ataatcaatg
     2821 acacagtagg catcatactt tcgattcctc cgcgactaag cagtgaatcg atttgttggt
     2881 ttccagtatt tgaaacaaac cctgattcga tgaccccagc caacttcgtt aaggaagttt
     2941 gtgggttttg aataaaaatc attcctactg ctacagcgat attcaacaaa atcgtcaaaa
     3001 ttgccggcac ctttttccat gcacacagta acattaaaaa taaaggaatc agtgaccaga
     3061 ttgaaatagt aaagtttgct tctagaacat cagtcacttc acgaatagat gaaagacttg
     3121 cggctttact tgtgtgccct aaaatcatga ataaaaagaa agaaacaaca aatgccggaa
     3181 cagttgacca catcatatgc ttgatgtgtt tgaacaaatc ggcatcaaca atagctgctg
     3241 ctagatttgt tgattcggat aatggagaca tcttatctcc aaaaacggca cctgaaacaa
     3301 tagctccaac aaccagcgct ggatttaggc ctagtgttgt cccaataccg aaaaatgcaa
     3361 tcccaatcgt tgacataaca gtaaaggcac tccctacagc gctacctacg attgcacaca
     3421 caacaaatac agaagggaca aaccactgga cactgatcaa tttgaaccca atgaccatta
     3481 gagttggaat gatccctgct tgtatccata cagcaatcaa tgaacctact aaaataaaaa
     3541 taaagattgg gatgattcct ggtttgatgc cattgatgat tccttcgtgc agccaatctg
     3601 tcgggtatct ttttatcact gcatacaaca tgaccattcc gatcgctgct aagatcgtga
     3661 tattaggtga gacctttaat ccgacaacgc caatcgcaat gcctaaaaca ataaccatta
     3721 atacaagtgt tgcttctaaa aaacttggtg ttcctttttt tcccatgata atctctcctt
     3781 ttttagttgt tcttatgttc taaaaaagtt aagatatcgg attactccgc cgcaattcat
     3841 ccgctattcc tcctttataa aaataaaaag acctgtttta aactaaggac gaatgactcc
     3901 gcggtaccac cttaattaaa aacagatctg tttttcactt tcaaactttt attcaaatgt
     3961 atggactttc catgtaattc gtcaattagg cttgatcggt tcacagcgac caccgacttt
     4021 ctgaacaaaa cacctaaccg ctactgcgtg aaaagttgac ttgtatgaag tttaccttcc
     4081 ataagatcat ttgtcaatga aaaagaaaat attttttcat ttttctgaaa attattttta
     4141 taactttcac acacaagttt attttttact agaaagtagc accctcttgt ttttttcttg
     4201 ctactatttc ttgatgttct tgcttgattc tttctaatag ctttggctct ttcaacagat
     4261 caagagctgt attggcgagt agttctgctg caatcgcaat agaagcaagg cctttgtcac
     4321 ttttagcagc agctttgaat tcttctgtgt gtccggcaat atatgtctca gagattgcta
     4381 ccgtaggctg gattgttggt atgacttggc tgacatttcc tacatcagaa gaaccgagac
     4441 ttgttctttg ttccgtttct atttcttcat ctggaacacc tgcttctttt gcatgctgga
     4501 agaataagtc atcaaaagaa ggagtaacga tgacatcatc aactgcattt tggaacaatc
     4561 caaactcaaa agtcgtgccc gtacttatcg cagcacctcg tacgatattt tccacttttt
     4621 catatacttt atctaatgtt tgccgatttc ctgcacgtaa atagaaacgt ccagcagcgt
     4681 aatctggaat gacgttggca gctactcctc cgtctgtaat gatcccatgg atattgacat
     4741 cttttggcaa ttgcaagcgt agagcgttga ttccattaaa cacttggatc aatgcttcca
     4801 aagcatttac tcctttttca ggtgctgctg ctgcatggga agcgacaccg taaaatttga
     4861 tgtcgactgg atcattcgct aaagattctg tcgttttccc ataacgaaaa gctggatgga
     4921 cgcataacgc tacatcgaca tcctcaaaaa aaccttctcg aacaaagctg cctttagctg
     4981 atccgttctc gcccccttct tcccctgggg tgccatatac acggatttct ccaccagttt
     5041 catcaataaa ttgctccact atgctggcgg ctaatacgga gtaagtacca aacaaattat
     5101 gcccgcatgc atgaccaata cctggtaaag catcgaattc tgccaaaaag gcaattgtgg
     5161 gtccgggttt attggaaata taacgtgcat caaaggctgt tcggtgtcca gctacttctt
     5221 tttctaccca aaacccttct ttttctaatt gtttaatcaa cgctgctgag gaataaaact
     5281 catagttact tacttcagga tgttcgtgga tatctagagc aagtgcttga tattctggta
     5341 aacgatattg gattagatcc ttcaattggt ttgttgagat tgttgttgtc ataaaaaatt
     5401 cctcctatgc tagtcatttt tcttattttc tcccgaatgt ttcccatgca ggcacgcttg
     5461 atccttttga tgttttctcg attactttga ctgtgtcttc tgtttgataa gcgtctacta
     5521 ttttttgata cgttttattt ttttcatctt cttttcttgc aacgatgata ttcacataag
     5581 gacgtgcatg gtcatccact ggttctaaga agatggcatc agaagcagga ttaaatccag
     5641 catctacagc tacaccgcta ttgatcactg aaatgtctac atctgacaaa gcacgtgctg
     5701 tttgggaagc atctaattca gaaatgtcta gattttttct attctcggta atatcagaaa
     5761 cagtgggcgc ttgtcctttt gccttatcca ctttgatcaa tccagcagtt tgcaataaca
     5821 gaagagctct gccgccattt gtaacatcat tagggattgc tacttttgct ccgtctttga
     5881 tatcagctac atccttcaaa gtgttagaat aaattcccag cggtgcattg acagtatttc
     5941 cgatcgatac taaatcagtt ccgtgttctt tattgtaatt gtctaaaaag aattgatgtt
     6001 ggaaagaatt caaatcaatc gatccatctg ctaaagccgc atttggttga ttgtaatctg
     6061 taaatttcac gtattccaaa ttaatgttct cctttttcaa acggtctttc acgttgtccc
     6121 atacatctgt atcttctcca ataattccta gttttacggt agtttctttt ttgtcagttg
     6181 tatctgcttt tccttggcta caccctgcca accaaataat tgctacaaat gtaatgacta
     6241 caaaacgaat aatttttttc attttttctc ctccactttc aatgtgtaat ttttttaatc
     6301 agttgattac tgataaattg acttaaaaaa actaagatta gaatcaataa tgtagccaca
     6361 atcgttacat ctgtttggaa tcgattatac cctctcgata tagcaagatt ccctaatccg
     6421 cctgcaccta ctgctccagc catggtagtc agtccgatca cgttgatgat agtcaacgcc
     6481 gagacccgaa tgataccagg caaaccttct aacaaataca cacgtaaaat aataccgatt
     6541 ggactggttc ccatcgcttc tgccgcttcg atcaccccag gatcaacttc tagcagtgcg
     6601 ttctcgattt gacgagcaaa aaacggaaca actccaaaaa tcaatggcac taaagctgct
     6661 ttcgcaccaa tcgtcgttcc aactaagaaa cgagtagtca atgctaaaag agaaagcaag
     6721 ataatgaaag ggatggaacg aatgatattg atcagcttat caagtatttc aaatagcaga
     6781 cggttagcta gtaatccatt agggcgtgtc actactaacg tcacacctaa gatgattccg
     6841 atcatcccgc caatgaatgc tgtccaaaaa gacatatata gtgtttgctt cgtcgcttcg
     6901 acaaattcat ccggaatcaa ataaacattg ggcaaatatg ttttaaagaa atcagtcatt
     6961 ttatcttcct ccttcgatca ctttgatacg actatctaat tcgttttttt gtaaaatatt
     7021 tatttttaca ccagatttga tcaaataatc caatgctttt tgccgctgtt tggtttctcc
     7081 agctaaagta acgatcaaac tccctaacgg cgtttcttgt aataactcta cattgccata
     7141 taaaatattc gctgttactt gatatttgct gtacagatga gcgattagag gttcgttcgt
     7201 ctgatttcca atataactta attccaccag ccatttattg gcgatctgat cggcaaaagc
     7261 agaatgggca atgatcgttt ctaatgcttg gtcaagatga gtagctgttc gaataaaatc
     7321 tttggtcaat tcttcttcag gattgctgaa gatctggatg ctactacctt tttcaatgat
     7381 tcttccatct tccataaccg ctaccttgtt gcagatttct ttgacaactt gcatctcatg
     7441 agtgatcagg acgattgtta aacctaactg gcgattcaat ttttttaaca atgcaaggat
     7501 ctgaagggtc gttttcggat caagtgcact ggttgcttca tcacataata ataccttagg
     7561 ttcattcgcc aaagctcgtg cgattgctac tcgttgtttt tgtccccccg acagttgttt
     7621 aggataagca gatgcttttt cttccaaacc gacaagttcc agtagttcat tcactttttg
     7681 acgacgttcc tgtttcgatt ttccagaata ctttaaagaa aaatcaacgt tgtcaaaaat
     7741 agttcggctt tccattagat tgaaatgctg aaagatcata ccgatggcct ttctttcttt
     7801 tcgcaattct ttgggagaga gctgactcaa attcgtctga ttgacaatga cttctcctgc
     7861 tgttggtttc tgcagcaaat tcatcacacg aactaacgta cttttccctg cacctgagta
     7921 accgacgatt ccataaacat cgcctttttc aatcgttaaa tctacagaat ctactgcctg
     7981 tatctctttt tctttttgtt tgaaacatac agaaacatct cgcaattcaa tcattaaaat
     8041 tcctccttat ctaaaaaaag cgtcctcaag aaaatgaaaa tgtcattttc ttgaggacgc
     8101 ttttttgcgt gttaccacct catgttcaag ctgtgttcac acacaactcc tcttcaagta
     8161 cgatcatact ctagcaagat atcggtcgca gccgtcatcg ccttacatct atgctcggga
     8221 tgaattgctc gatggccatt ttccaatagt cgttccttgc ttcttctcag ctaccgaagc
     8281 tctctgtcaa aggttcttct atcgtactta ccatttcaat gcaaataata ttcaaaaaaa
     8341 aatcctttta ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt
     8401 tttcggaagt ttccttccct caagcccaac atctcaaaaa gagagattgg cgttcgtttt
     8461 aacggtcgaa cagttccgaa tttgcctact tatcgacaaa ttattctcca aggccatttt
     8521 cagcttttct tacttgactc ttctcaccag ccagagcctc tctgcaaagt ttccaaactt
     8581 actttccttt tcaacgaaat cgatattttg ttgttgggat taatctacat gaggaaaact
     8641 aaaaagtcaa gcacgaaatt caaattattt tttctatatt tatttttctg tttttttatc
     8701 taacatttca ataacagttc gaagatcttt tccttcagca agaagtttca atttttcctt
     8761 ttcttcgacg gtataatttc ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc
     8821 tgctcgtgaa gcagctgttg ctcctactaa agaatcttcc actgcaaagg ctgaactagc
     8881 ttctaatcct gttcgttcca aagctgtcag ataaggaatc ggtgaaggct tgttctccga
     8941 cacttcgtct ccgaatgtaa acgtgtcaaa gtaatcagct aatttaaagt gatccaagat
     9001 atctgttgct cttttcgtaa cagtggtcgt agcaatgcct agtttcatat gacgttcttt
     9061 taagtctgcc aatatttctt ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg
     9121 ttgataaaaa tattcttcac gcttcgctcg gacttcctgt actttttcag ggctacctgc
     9181 tgctttcact aaaatatcaa aactttgttt ccagtttttt ccgccccaag cgtcaacgat
     9241 ttcttttggg atatggatat caaattgctg aaatccccaa agccagccat cacgatacgt
     9301 tttttctgtg tcaactaaca aaccatctaa atcaaatatt gcacctttca tctatacatt
     9361 aatcctttcc aaaacaatac acatagttta gcacgactgg tccaaaaata acagaagcaa
     9421 agaaccagcc ttctcgttta taagagttct tggaatcgat ggatatagat tttcttcaca
     9481 gctgtcacaa gcgccagata agcgatgact gtagcaaata accataacca gtaattgcca
     9541 ggaagtggca tcaagcctaa ttcagcacca aaacttgtaa aaggaaggac agaaccgaca
     9601 ataattccta atgtagtgat aacaaacatg gcaaatgtta cgttgctttg gataaatggt
     9661 actttcggtg tacgcagtgc atggagcact aaggtttgtg tccatagtga ttcgacaaac
     9721 catcctgcat ggaataatgc gatgaaaatc acttgctgtg aactatccaa tgtatggaaa
     9781 cttccgccaa ctacagcagg acaaataaca aaatacatca acaagtaagt agtaatatca
     9841 aatatcgaac ttgttgggcc aaagtaaacc atgaatttcc caatactaga tgcattccat
     9901 ttttttggct cgtccaaata ttctttatcc atccgatccc atggcaagga aatgcaggaa
     9961 acatcataga ttagattcaa aaacagaagt tgtaaaggca gcattggtaa aaatggcagg
    10021 aacatacttg caatcagcac ggaaaacata ttgccaaaat tagaacttgc tgtagccttg
    10081 atatatttca tgatattccc aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc
    10141 gtcaaatctt tttctaaaag gatcacgtct gcagattctt tcgcaatatc cacagctgta
    10201 tctacagaaa taccgatatc agccactttc attgcaggag cgtcattgat accatctcct
    10261 aaaaatccga cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc tttttgctga
    10321 ggattcaatt ttacgaatac ttcatggtct tcagctactt gtgctaattc atgatcactc
    10381 atttccgaga ttttctcacc ggtaaccaat tcattgatct ctagacctac ttgacggcac
    10441 actgaacgtg taactaattc gttgtcccca gtcagaactt ttacagctac accatgttct
    10501 ttcagagctt tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa agtaagataa
    10561 ccaatcaaaa ccatgtctga ctcatctttg actgaaaatt ctccaacgac gcttggattg
    10621 gtcttttgtg cgacaccaat cactcgtaag ccatcttcat taagtgactg tacttttgca
    10681 agtacggctt cttttcgttc ttttgttaat ggtgtaacag taccgccaag atcaataaat
    10741 ttagaaatgt tcagcatttc ttcaatcgcg ccttttgtga tcatttgtgt tttaccagct
    10801 ggatcagcaa ccacaacact catacgacga cgttcaaaat caaaaggaat ttcatccact
    10861 ttttgataat tgatcttatc tgtatctaaa gtttctgttg cttcatcaat gatagccaca
    10921 tctaacaaat ttttcagccc tgtttgatag tagctattta gatacgcatg gcgcaaaaca
    10981 cgatcatctt catttccttc acaatccata tgatattcaa gaatgatttt atcttgtgtc
    11041 aatgtccccg ttttatccgt gcataacaca tcgattgcac caaagttttg gatggcattc
    11101 aaatttttga tcactgttcc ttttttggcc atcacacttg ctcctttgac caaattcgtc
    11161 gtaacgatca ttggaagcat ttcaggtgtt agaccaactg ctacagatag tccaaaaaga
    11221 aaagcttcta gccaatcccc ttttgtaagc ccattgatga cgataactgc tggagccatc
    11281 aatgacataa atttgatcag tagcatggat gttttatgga tgcctaattc aaagcttgat
    11341 tgaattggtt tttcagatag cgtttctgct acatgaccaa atagtgtttc atttccaaca
    11401 gaaacaatga ttccttccgc acttccactt atgacattac ttcccataaa gacaagattt
    11461 tcgtaacttg tttcgctttg ctgatcgtct tcacatactt tcgcatgctt ttctactgga
    11521 tagctttccc ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga caataaacgc
    11581 atatccgctg gtatcatatc ccctgctgaa agccgaacaa gatctccaca gacgatttct
    11641 tccataggaa tctctgtgta ctttccatta cgttttacag ctgccgttac tttcacaagt
    11701 gatttcagtt tttcagccgc ttgattggaa cgtacggact gaatcaacgt cattgtcccg
    11761 ctgacgatga ccatagtgaa gataatggct acaccgaaaa gatctttatc tccaggagct
    11821 gctaatacat attccgtgat gaatgaaata attcctaatg cgatcaacac taatgtaaat
    11881 ggcgtgatgt atgctttcaa tatttctacg atgaatggtg tttttttgcc atatgaaatc
    11941 gcattttcac cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct
    12001 gatgcgtgga atagttcaaa taattcttgt ttcgtttttc cagcaaaagc tgcatagatt
    12061 ttttgtgtat ctttcttttt ttccatctca atctctcctt tgtgtccaaa agacagctgt
    12121 tctcgctgct tttggtcttt agctattcat cggatcgata gagaaaaaac tgaaaggggc
    12181 tgagaacaga tagataatag ccttggcctt tcctctagat ccttgttttt cgaatgactg
    12241 tcatccattt gttctcatct cctttactaa gtaattttaa ttcatgactt ccaatcattt
    12301 ttcgaatagt tattttccca attagaccac ctccttttta tttttcaccg atggggattt
    12361 gcggcaacaa aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa
    12421 aaaagcttct cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatacc
    12481 tgcttttagc taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt
    12541 ctctcgacgt ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatctta
    12601 ttcggttttc cttcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg
    12661 tttttcatac tttttttaaa atacacacgt tatattcctg tctcacgcgg ttttatttgt
    12721 ctcattttcc acctcccgct ataatagaaa tatagtagga aacgaggtgc tgttatggaa
    12781 agaacacttg ttattatcaa gccagacggc gtgagacgcc atttagtcgg aagcatcatt
    12841 caacgttttg aagcaaaggg actggccatt gctgaaatga agtttgaaac tatgactcct
    12901 gaattagcaa aagaacatta tcagcacttg actgagcgca gtttttttga tgaactgatc
    12961 gattacatga cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat
    13021 atcgtccgta agatggttgg ggcaacaaaa gctgtcgatg ctgttccagg tacgatccga
    13081 ggagactacg cgctgccggg gactgaaaac atcattcacg cttctgattc ccgtgatgct
    13141 gcggtaaaag agatcgcccg ctttttcccg ccattcgata taaacaaacc gtctgaaaaa
    13201 gcggcgaagt gatacaaatt ctaaactgct ggtctagttg ctgggttcag caatggccag
    13261 cagttttttc tctaaggacc tccgttgatt ctttcctaac atatattcga gcactgtgaa
    13321 gaattcttgg tagtctttcg tctttttttc ccctaaagca gcagaaatca atctttgata
    13381 ataatctgcc atcaatgttt catagatcac tgtacttctt tctttatgtg ctaagctccg
    13441 tttcatatct tcattcacac tacgtgcctt tttcaaccga ccattttcta tataaaacga
    13501 agtcaggttc attaaaaaag taaagagata ctgctcatga ttgttcaact gtgctaatcg
    13561 tcgcattctt tttttgattt tcaaataatt gttctcaatg aaccgatcac tgaatatcca
    13621 aggaaaatca gctagaatag atagttcaaa ttgaccatag tctggtatct gcatcaagta
    13681 ttcttcaatg attcggcatt cataacgtaa aaattcaaat tctgtctcag atacttctgt
    13741 tttttcgacc atgtgaagac tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg
    13801 cctacggtta ttgctgatct ctttataagg atcaaagctg tcttcttgta cccgatctag
    13861 cattcgtctt tttttcttta actttattgg ttcatccagc tggtttcgca tccattcata
    13921 ttctgcaagt gtcatgttca ttcgctctaa aaatgatctc aatgttacaa atgacatttt
    13981 tttcactgaa tttgtctcaa tcccgactag agaagtctta gaacagattc cttccgctaa
    14041 ttgggactgt gtcaacttct tgttgactcg aagtttcttg atcaattcac catcatacat
    14101 aatagttctc ctttttaatt taaatttttc cacattcaat aaatagatac gttaaaaagg
    14161 aaaaatacac gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa ccttcaagca
    14221 ttagaatgtt gtctaactac tgaaggttgg tttgattgtt tattgattat ctttttgctg
    14281 ttcaacgtct gttcttgtta ctggatataa gttggttgtt gttttattgc cattttttga
    14341 aaagacactt gtcgaatgtt cgcctttttg cttttcgatg gctatgttac gttctgcttc
    14401 atttttatag tcatattttt tcggatcgat cgtttcaaat ccgtttggca cataaaaacg
    14461 caagagattt tcttgattca gcttatctga aagacttaaa gcagatttga ctttttcctg
    14521 atctttggaa atttcctcgt cgattcctgc tgctttcgta tcgatgattt ctcctgtttg
    14581 gttattgtaa gcttttcctc ctaagatggt gtatttgggt gtcacgaagt tcccatttcg
    14641 aaaagccact acttgatcat gctgaggaga taataaatcc gtaccaaaat ggagatagtc
    14701 tttatcatcg attcccaaga gatgaagtaa tgtcggcaaa acatcgattt ctccaccgta
    14761 ttcgtgattg actgaccctt tagtataacc aggtatatga atcattaatg gcacacgctg
    14821 catttgtgcg ttgtcaaatt cattccatgt atctgggtct tttccaagag cacttgctaa
    14881 gtctttatta tctgtatttg agatgccaaa atgatcccca taaatcacaa agatcgaatt
    14941 ttggtaaata gcggattttt tcaggtacgt aaaaaattgt tccaatgact gatccaaata
    15001 atgtgccgtt cgaacatagt tatctactgt gctatttcca gtgttcaaag atggaaaatc
    15061 gaaattctta tcatccgtat aataaggcgt gtgattggtt acggaaagga atttcgtgta
    15121 gaacggctgt tgtagatgtt caagatattg ggcagattca ctgaataaat ctttatctaa
    15181 tattccataa cctagcgttt catctgattc atcaaaataa gatcgatcaa agaaattgtc
    15241 ataacctaag tttttatata catgatctct attccaaaaa ctagctacat tcccatggaa
    15301 aacagcgctt gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt
    15361 atctgaccca agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa
    15421 catattttca gcatcacttg tctttccctg tcctacttca tgaaaaaaat tatcaaagct
    15481 gatcgttgct tgattctgga aaatactatt taaaaacggt gtgacttctt gtccgtctac
    15541 cttcatatta atcaaaaact gctggaaact ttctaaatgg agaacgatca catttttccc
    15601 tttagcgatc ccaaatgttt cgggatttgg ctctgcataa tgtttctttg tataatctaa
    15661 aacttcatca atcccattac tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt
    15721 gattccatca taaatagtat acgcatcgat tcctagatat ttgacaatat atgaacggtc
    15781 aaatgttcgc tgcagcaatt gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa
    15841 aataagccct gaaagacagg tcacagcgat agccatcggt ttgctgacag gttgactttt
    15901 gataggtatt ttcttgatcg tcaaccaaac cagtatccca atgaacgtag caatatccag
    15961 ccagtagagg acatcatggg gtttcatcaa agaaaaggag cttcctgata acccttgcga
    16021 cactttcgaa aatcccaata cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa
    16081 aataatgttc aagtacaaca aaagcgtgtt taaaatatca atgatcaaaa gaatggctaa
    16141 tgctggcttt ggctttttaa tatacaaaga aaggccaaaa aacaataagg tagttgcaat
    16201 cgggttgatc cacaagataa agtactggat cgtaccttcc acacctaacg aaaaatctgc
    16261 atagtaagca ataatcgtct tcatccagaa aaagaaaaca gctgtcaaaa cgattgctaa
    16321 acgattttgg cacaattttt taagttgtgt cacgtatatc acttctctct attcacattt
    16381 ttccttattt tgctcctgaa aaatgaaaat tccctaaaaa agttgttatc ttaagatttt
    16441 tttattcttt ctaaagatta ttccgtgcac ttttcacagc ataaaaccgc tgtgatataa
    16501 gcgattgaaa cagtaaggtg ataaaagtct tgtcgcagct cctgtttagt tgatgcagga
    16561 cagctttttc acaacttctt ctttaaacgg tgttctcctg ccaactcctc ggccacaagt
    16621 cacaatattt ataaaacatg agaagctgtt ttttgaaatt gttccttgtg gctcagagtt
    16681 aaacggcaag ctcaagacct cttgata
//
LOCUS       DAZKNO010000050        16460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000050 DAZKNO010000000
VERSION     DAZKNO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.050"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1183
                     /locus_tag="ACG8MY_001233"
     CDS             155..1183
                     /locus_tag="ACG8MY_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430442.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1209..1649)
                     /locus_tag="ACG8MY_001234"
     CDS             complement(1209..1649)
                     /locus_tag="ACG8MY_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0430443.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1809..2315
                     /locus_tag="ACG8MY_001235"
     CDS             1809..2315
                     /locus_tag="ACG8MY_001235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0430444.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2483..4396
                     /locus_tag="ACG8MY_001236"
     CDS             2483..4396
                     /locus_tag="ACG8MY_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0430445.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            4401..5402
                     /locus_tag="ACG8MY_001237"
     CDS             4401..5402
                     /locus_tag="ACG8MY_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0430446.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5604..5810)
                     /locus_tag="ACG8MY_001238"
     CDS             complement(5604..5810)
                     /locus_tag="ACG8MY_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0430447.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(6456..7304)
                     /locus_tag="ACG8MY_001239"
     CDS             complement(6456..7304)
                     /locus_tag="ACG8MY_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0430448.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            7468..8370
                     /gene="gnd"
                     /locus_tag="ACG8MY_001240"
     CDS             7468..8370
                     /gene="gnd"
                     /locus_tag="ACG8MY_001240"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0430449.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            8372..9373
                     /locus_tag="ACG8MY_001241"
     CDS             8372..9373
                     /locus_tag="ACG8MY_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0430450.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            9370..9654
                     /locus_tag="ACG8MY_001242"
     CDS             9370..9654
                     /locus_tag="ACG8MY_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0430451.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            9687..10484
                     /locus_tag="ACG8MY_001243"
     CDS             9687..10484
                     /locus_tag="ACG8MY_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0430452.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            10498..11331
                     /locus_tag="ACG8MY_001244"
     CDS             10498..11331
                     /locus_tag="ACG8MY_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0430453.1"
                     /translation="MEILSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            11349..12206
                     /locus_tag="ACG8MY_001245"
     CDS             11349..12206
                     /locus_tag="ACG8MY_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0430454.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            12228..12737
                     /locus_tag="ACG8MY_001246"
     CDS             12228..12737
                     /locus_tag="ACG8MY_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0430455.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            12774..13175
                     /locus_tag="ACG8MY_001247"
     CDS             12774..13175
                     /locus_tag="ACG8MY_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430456.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            13287..13640
                     /locus_tag="ACG8MY_001248"
     CDS             13287..13640
                     /locus_tag="ACG8MY_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430457.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            13674..14117
                     /locus_tag="ACG8MY_001249"
     CDS             13674..14117
                     /locus_tag="ACG8MY_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0430458.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            14134..15219
                     /gene="uxuA"
                     /locus_tag="ACG8MY_001250"
     CDS             14134..15219
                     /gene="uxuA"
                     /locus_tag="ACG8MY_001250"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0430459.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            15212..15925
                     /locus_tag="ACG8MY_001251"
     CDS             15212..15925
                     /locus_tag="ACG8MY_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0430460.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(16132..16281)
                     /locus_tag="ACG8MY_001252"
     CDS             complement(16132..16281)
                     /locus_tag="ACG8MY_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430461.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         5726 a         2570 c         3456 g         4708 t
ORIGIN      
        1 atcttagtca aaaaattata taagtttgcg tacgattcaa agaaaattaa gagaacttaa
       61 tactattttg aaaaactatc atttatcttg cagcttgaaa aaaggtaatc cgatagaagg
      121 agaagaatac cgtatccgat acttttttca cttgatgtat tggcagtcat tcgacgaaaa
      181 tcattctgat atattatcaa gtataaaaaa taaacgtcaa ctattagaaa aatttgcgaa
      241 acatgcgcca tatacgcgta gtatagatag aaagaaatgg atcagcctac taagaatttc
      301 gttgcaacga atgaaacaag gccactcgat tacgtatgtc ccagaagaga tgaacaaatt
      361 tgtgcatcca ttgataacaa aagaagagtt tacgcattac atactagagc ctttttttga
      421 agcaaacttc tggtcgatga atcacatcac tccaacagag caagcttacc tttattttat
      481 gtttggtgta atgaacacct atttggaaga agatttgttg gatactccag ataaagtaca
      541 agaaagttgg ggctggattg aatcgtctat tgtagaaaat tttgaaaaaa acttcaagat
      601 ttgttttaca acttcagaaa aaaattatct atatgtcaat ttggcaatga tccatttgta
      661 ttcaagggtt tttgggacaa aaaagaaggt ggatgctttt ggaaaatcag cagaagacag
      721 agattttcag catatatttc cagtgatgta tccaatcatg caacagtttt ttagtggggt
      781 agctcgcaga attccagagc ttaatactta ttatgaaaag aacagacggt tgatttttca
      841 atactgtatg ttagtcaggg atatttttat aaaacacgaa caacctgtca tattctatat
      901 tcaatcaaag tatggtaaaa cacaggaatt atgggcaaag aaaaggatat tatctatgac
      961 tgaacgccca ataaaatata gtgcatccgc tgatcagtta cctgatattg tcattagcga
     1021 ttatcctatc gacaaaaagg catatgatca aacgaataca cagatatttt actggaatgg
     1081 acaacctaca aaaaatgatt gggcgcgtct gaaagctgaa atcataaaaa tcaggaatga
     1141 gaaaaataga tccatattgg aaaaattgga tttcgttaat taagaaaagc agattgttta
     1201 tgccaaaatt aagacattgc aaaaagatta ttttctttaa aatcgacaag atgaccagaa
     1261 agcaagagtt cgttgagctc ttgctttctt ttagttttag aaaggaacat cctgatttta
     1321 tcttcgctaa atccagtcaa aagttttgca gattggtcgt agataatcgg ttttttcaat
     1381 aaagaagggg atgtacgaat ctcaatatat agttcactca aactgagttc atctattctg
     1441 tgaacaagat ttttataact aatagaatga gtagagagca tttcggtcac gccgttgttt
     1501 gtcaatttta gtaagtggat aaattcattt ttggttagtg gttgacgaat taagttctgt
     1561 tcgataaacg gtatatcatg gacgataagc caacgttttg cgcgtcttga agacgagcag
     1621 ccagaacctg tatacaattt aatcatcata cattcctcca atagctaagt atttatgaaa
     1681 taataacatg aagaaatagt cgaaaagtgc catttagagg tgtaaaataa gaaaatgttg
     1741 tcacgtataa tggaactagc tagataagaa atactattaa atcaagaaaa aagaaagaag
     1801 gcacaagtat gtcaaacaca gccgacgcaa tagtagtgat cgatttacaa aaaggggtat
     1861 gttttgattc ttcgccgata cacgaattag aaacagtgac agcattagtg aataaaagaa
     1921 tcaaaacata cgaaacgatt gggaaaccag ttattttcgt tcaacacgaa gatgctcaat
     1981 tagtgccaca aacaaaacca tgggagctat tgtcggaatt atctattcct gagcacagtt
     2041 attttgtgag aaaaacccat gcgaattcat tttataagac taatttgagt gttttattaa
     2101 ctgaattgga ctgtcattcg atcgaattat gtggggcaca agcagagtat tgtatagata
     2161 cgactgtgaa gtttgctcat ggattaggat accagcttat gatgtgtaga ggagcaacga
     2221 caacttatga taatcctttt atgaaagcaa aggaaacaat cgctttttac gagaaaattt
     2281 gggataaacg atttctgaca tttttggagc aataagttgt ttcttcgttt aaaaaaagca
     2341 gaatggatac tggtttatcc attctgcttt attacgtaaa atgagaaaat aatcctgttc
     2401 tatttttatg ggataaacca tttatttttt gtataaaaaa tgtttgcgtt tacatataat
     2461 tctcctagaa acataaaaag gagtgaatag cttgagaaca aaacaagtat taaagggagc
     2521 gttggtcatc ttatttgctg taagtacaag cgtcccgcaa tggacagtat atggagagag
     2581 catttcttcc gaatcggaat ctcgggaggt agaaaccaca gaaactactg atgctggaaa
     2641 tgaaacgaaa tcgagtactt cacaaacaga aacaaatact gaaacaacgg ttgagacatc
     2701 tgttactagc acagatcaag aaacgaccgc taccacagat aaccaacaaa cagaagaagc
     2761 aacagctaca aaaatgtcaa acgacgaatc ctcttctgct aaagtaagtg acaaagagaa
     2821 tgggacagaa aatgatgata tcggtaaagg tgaaaatttc aatggtaaag aatatttgaa
     2881 ttacttcggt aaacagatga cagaaatact taatggacca acagaagaca tcaagtcaaa
     2941 attaatagag ttattagtca ctgagcttgc tgttaatgat tatggtgtag ataaagagat
     3001 tatcaaacat ctgattgcta cttatctatt gtacgatatc gcttttgcaa aaaacagtgt
     3061 gttgatgtcg atgcttggag gtacagatga tgggacaacg cctacagaaa acacaggctt
     3121 ctatccaaat acaaaacaac ttcatcaaac tgtactaaac aaacaagatg gcaaaatgat
     3181 ggatgtatat gcatattatg tcgatcaagg ttcagataaa acagtcatga tccatggcgg
     3241 atttcgaggg aattggaata acgggatcgt tacagaagaa tacaatgatt tttataaggc
     3301 cgggtataat ttattatttg ttgattccag agcgactggt aatagcggag gcgattatgt
     3361 gacctatgga caatatgagt cagatgacgt cctttactgg ataaatcaag aagttcgaga
     3421 gcggccatct caaaagattt tactttatgg tggttcgatg ggagcagcta cgatgatgtc
     3481 tgtattagcg aaagatattc ctgtcaatgt caaaggaatt attgaaaatt gcggatttgc
     3541 atcaattgat gaacaactac gttttacgta cagccagaca gtagcccccg cactaccgga
     3601 tgccataaaa aatcagttag acatcattgg ggatcaagag catgaagatt tatttatggg
     3661 ccttttgaaa caatactatt ttgatcagga gatgcatttg gatacaaaag ctgctctacc
     3721 tacgattgga atgtcagatt cattgcctaa attgattatc catggaacag cagatgatgt
     3781 agtacctgtg tctaacgcgc aaaaattata tgagttatca ggagggtata aagatctttt
     3841 attagtagaa ggagcagggc atggaaaggc acaggaagtt gaccatgctg cctacacgaa
     3901 acatgtaacg gactttttga aagtagtgtt ccatgatcag ataaacgtaa agtatgtgga
     3961 tgaaaacaat aaaagtctac tcaaagacca ggacgaaatc cagctttacg gagcttatgg
     4021 agaaaattat atgacagagc aaaagacatt cgaaggatac gaattagcaa atgttgaagg
     4081 accgactgaa gggattttta atgagacgat accaactatt attttctatt acaaaaaaat
     4141 cccagtagct ccaccaaaaa agcaagatcc gacagagaat gcagacaata agggaaagaa
     4201 tgaacctcga gaggatcaat caagtgatga tacgcaaccg attcagatga cagtgaaagt
     4261 acaaaaaaag acagatgcaa aaaaaagaat gttgccgaaa actggagaaa agaaacattc
     4321 gcttctcatt tattcaggtg gatttttatc tgttagcacg attatcacat ggatttggcg
     4381 aagaaagaga gggtaaatga atgaaatggt tcaagcgaat agtgattgca gtaccagtta
     4441 tcatcgtagt tattttagtc actgtatttt tgattaatca aatcacacca aagccgatta
     4501 gcttgcttgt tagaaaacaa tttgacacaa gtgaaaaaga acaagtatat gcccgaccag
     4561 atgatttcca gcaaaaaatc gaacaaatcg atataaaaaa agaaatatcc tatccttcaa
     4621 agtacaaaaa taataatatg gatatctaca caccgaaagt ggaaaagaaa aaattaccga
     4681 ttcttttctg gatgcatgga ggcgcatatg taggagggga taaaaatgat tgtcgtgatt
     4741 atttggaata tctatgttca gacacgcagc aaatcatcgt aaatatagat tatgaacgat
     4801 caccggaagc aaaacatcct acaccagtta tccaattgaa cgaagccatt caagcagcta
     4861 aaaaagagat aaaagatcaa gcagattggt cgaatatttc gattggtgga aattcggcag
     4921 gtgcacaaat tagtggagaa tatttgttag cacttcaaaa tgaagaaatt aaaaaagctg
     4981 atcatcttga tccgactttg gagaacaagc agattacgaa gtttatttct ttgtctggtc
     5041 tgttggatcc ttctgcactt actcaagtaa gtgataaaat ctctagcttt ctttatgaaa
     5101 aatgtggatg ggcttacttt gataataagg actttgaaca atcagagaag gtcaaaagtt
     5161 tagccttggt cagacatgcg gatcgttgga cgcaaaacac attcttaacg gatggaaata
     5221 caaatacgtt tacgaaacaa atggaagaag taacttctgt atttgagaaa gcgggggtga
     5281 aagtcacaaa agtagattat gcggagaaaa atgctgtttt gaaccatgaa tatcagtttg
     5341 atttcagcaa taaacaagcg caagaaacat atcaaaagtt agtaatgttt ttcaaggagt
     5401 aaagtcttat gatgcaaaag aggttgtaat ctgtcactta gctccaagtc acaaggagca
     5461 atttcaataa acaagctttt catggtttat gaatattgta acttgtgact gaggagttag
     5521 agatggcaag gggcaccttt tattcgaaat ttgggaatat cacacgatta ttgtcacgat
     5581 ctcttttacg taaattttat aagtcagatt tgttgttttg atcgtttaaa aaaagcaaca
     5641 aatccgaagc ctgcggaggc aagaagggac aaaaagccga gtggtaagca aaagaatgct
     5701 ggttccacta atattccgtt gttattgata taaggctggg aacaaagatg aatagcaaat
     5761 aaaatcaatg cactagcgaa aaaaagcaaa gaaatatgga tagctttcat aagcagcacc
     5821 tcctttttta taattgtatg ataaatagat taagaatcta taaatgtgac aagtttgtta
     5881 ggatgtgaat aaaatgatca tctagcgaac ttgaaacaaa gatggctaat gataagcaat
     5941 taatgattca gcaatcagat aggtacaaag tttaccatca tacgtctaga ccaataatag
     6001 aaaatatggg aatataatag aaaagattta ggataccccc aaaagaattt ccccaaaaat
     6061 tcgatgtatc ctaaactttt ttattttaca taacaatcat atcaactgtg aaataaaatt
     6121 acaaatgata acctaaaatg aggccaaaag ccagtgttat caaggaaata cttaattcac
     6181 caaaattttt tttaattttt tagaaaataa gaaacaaaaa taataaaaaa aagaaaagat
     6241 aaatttttta ctactgtata ctaactttgt tatcgcatct aacaagtaac tcgaataata
     6301 ccaatacaac cttctccaaa gatgcgatga catacgttct aacccaccga ctattgatgt
     6361 tctgtaccac ccttgcagaa ccaaaaaagc cactaccaaa tatttggtag tggctttttt
     6421 tgttttatgg atttttcgct ttttctgtta tggtgctatt ctttgatttc agaaatagta
     6481 tgtcgaactt tagcgataga acgacgtgat tctttttcgt tgtaaaacat caaaatcaca
     6541 tatagagagt ccaaaatgct tagttgagaa atacgggaag ctaaagcttc agatcggaat
     6601 tctgtttctt cagaaataga aataaaaacg acatcgccaa gtttagcaag ggaagaatta
     6661 gcttggctag taataacgat tgtttttgct ccagtttttc ttacggtttc agcaatatgg
     6721 attgtttctt ttgattgccc tgtgtgagaa atcaaaacag cacagtcttt ttccgtcaat
     6781 aaagaagctt ccatcaattg aatatggtaa tccgtgctgt gagaagttgc gataggggaa
     6841 cgcaaaaatt tatgataagc gtcagttgct acaatctctg aaccgccaac accgaaaaaa
     6901 tagacacgtt tagattcatt gataatagta gccgcttttt ttagatcctt ttgttgtaac
     6961 aacttttttg tgtcatttaa agttgaaata ctagactcga acaccttttg cgccatagtg
     7021 agctcagtat ctgttttctg aatattttcg tggatagaaa tagcagatag atcattttct
     7081 tggatcaaca tggccatttt aaaatcttta aatccattat aaccaagttt ttttgtaaat
     7141 tgaaaaaaag tagagtcagc aatgcccagt tcacttgcta aattgctaat aggactatga
     7201 cttactttgc ttggattttc taaaatataa tcggcgatag cctgttcttt tgggctgaag
     7261 tcgttgtaca ttgttttgat ttgtagtctt atggagacgc tcatgagaat tcctcatttt
     7321 cataataaga ttatcatttt ttccgttctc attaatgtta aagatacaag gagagtagga
     7381 aaaaatcaaa caaaaaatat ttttgtaaaa tctatttaca aaaatatttt tttgtataat
     7441 gagggtataa gaaatggagg cgctatcatg gaaattggtg taattggttt gggaaaaatg
     7501 ggattgaatt tagctttaaa cttaaaagag caccatcaca aagtacaagg actcgacatt
     7561 tcaagtacag ctgttagtca ggcaagagaa aatgacattc ctgcttatca agagatctct
     7621 gaatttctag ctacttttga gcagaaaaaa atcatctggt taatgcttcc tgctggagag
     7681 ataaccgaaa acatgttaag aaaacttttt ccaaaactcg gaccaggaga cattattatt
     7741 gaaggcggaa actcttatta taaagattca atccgtcgag cagaagagtt tcagaaaaat
     7801 gaaatcggtt acttggattg tgggacttct ggaggaatcg aaggagcacg acatcaagcc
     7861 tgcttaatga ttggaggaga taaagaaact tttctatccg tggaacaatt attcaaagat
     7921 gtagcaattg aaagaggata tctgtatgct ggtgcatccg gaagcggaca cttcttgaaa
     7981 atgatccata atggaatcga atatggaatg atgcaagcaa tcggtgaagg attccaactt
     8041 gtacaaaaaa gtgagtatgc ttttgacttg gaacaagtcg cacgtgtttg gaatcatggt
     8101 tcggtgattc gcggctggct gatggaaatc atggaagatc agttaaacga acatcccaat
     8161 ttaagtaatt atcggggaat cgtttctgca tcaggtgaag caaaatggac ggttgaaaca
     8221 gcgctagaaa tggctgttcc agttccagta attgcaacta gtttgttcat gaggaatctt
     8281 tcacaggaag aagatagttt ttcagcaaaa gtagtttcag cactaagaaa tgggtttggc
     8341 ggacatgaaa tagttgaaaa gggtgagtaa aatgccagat tatcatatag caatcgttaa
     8401 ttccagcagt ttcggaagag tctttccaca ccacttggaa agattaaagt cagttggaga
     8461 cgttcatttc tttactgttg atcaagagat tccaggaaaa gaactagcgg agttacttaa
     8521 aggattcaat atcattattg caagtgttac gccatttttt cgacgagatt tttttgaaaa
     8581 caaagatgaa ctgctattga tttcccgaca tggtattggt tacaacaata ttgatttaaa
     8641 agcagcaaaa gaacaccaga caatcgtatc tatcatacct gctttagttg aacgaaatgc
     8701 tgtagcagaa aataatataa ctaatttgtt gtccatgctt cgacgcacga cacaagcaca
     8761 agcaagagtc aaggacgatc gatgggaaga tcgggcggag ttcgtcggta ggacgctgtt
     8821 taacaaaaca gttggtgtga ttggtgcagg aaataccgga agcggagtaa tcgaaatttt
     8881 gcgtaacggt tttcgctgtg atgttctagc ttatgatcct tacaaatcag atttatacat
     8941 cgaaagcttt ggtgcaaaaa aagtagaact tcaagaatta ctggaacaat cagatatcat
     9001 ttgtttatgt gcgaatttga cagaagagaa ctatcacatg atttctgata aagaagtcga
     9061 tcagatgaaa gaaaatgttt atctatctaa tagtgcaaga ggcgctctag ttgatgaaga
     9121 agcgatgatt aatggtctga aaagcggaaa gatcgcagga tatgccacag atgttttaga
     9181 agaagaaccg ggacgtcaag atcatccgta tttagcgttt gaacaagtag tgattacgcc
     9241 acatatatcg gcctacacaa gggaatgttt agaagcaatg ggtgaaaaat gtgtcagaga
     9301 tgtcgaacag atcagtcagc accttcttcc tgaacgttct gtccagacaa agagcaacta
     9361 tataaaagga tgatgaagaa aatggtgaac ggtttaaaag tgaaaacagg ccctcaattt
     9421 tatttatatg aagaaggtgg tatttcaaaa gtttctgatc tcttgaaatc atacggcgcg
     9481 aaaagagtac tggtcaccca cggaacggta tcatgggaaa aggcgctacc taaattagta
     9541 tttttgaatg acgaaacgat tcaatttttt tatcatcgtt attctggaga atgtagttat
     9601 gctgaagcca gacgaatagc tacaatcatc aaaaaaatga aatcgatttc gtagttggcg
     9661 ttggcggagg aaaactgacc gatctagtgg gttatagtgc caatcttgca agaatcgatt
     9721 tcggagtcat ccctacttta gctagtaatt gtgctccatg gacacctctt gctgtcatgt
     9781 ataaggagaa tggacaagca gaagggaaaa cagaacactt ttacagacaa gctgcttttt
     9841 ttataacaga tccccaatta gtgattgatt cccctgttcc atatttcatt gccgggttag
     9901 cagatacact agcaaaatgg tatgaatctg agctcatctt agaacaagat tttttgcaag
     9961 atgaatcatt tttgaagcta gcacaagata ctgcgaagat ctgcaaagaa gagattttga
    10021 atcattctgc taaagcaatc caagatatgg cacaaagaaa actgacatcg gagttcaaac
    10081 agctttcaga gattatcttt gctgttgctg gtttagttgg aggattagga gataaatacg
    10141 caagaaatgc ggcagcacat gctatgcatg acgctatcag taaatatatt ccggaaagcc
    10201 atcgttttct acatggagaa aaagtagcgt atgggatttt ctatcaactg gcactagaag
    10261 gtcgctggga aaaaatagac gagttatatc ctttgtatcg ggaattagaa ctgccggttt
    10321 ctctagaaca aatgggatta tttccgaaag ataaacagat aatagataaa atggctgaat
    10381 ttattgattc gaaagagaaa gtgcatttga ttcctataga ggtaacacag aaatcactga
    10441 tacagtcaat gatggaactg gaaggttata tcagaaagat ctgaaggagg aggacacatg
    10501 gaaatattaa gtgtagtaca aagtttgttg attgctttat gggtagccgc ggttatgtcc
    10561 cgctggttag gtggaggagc cacgttgacc ttacgttttt ctcctttgat gactggatta
    10621 gtcgtcgggt tgatcatggg ggaagttgca caagcgatga ttgttacagc cgctttgcaa
    10681 atgatttata tgggggtttt ttctccagga ggatcaatgc cagctgaacc atctattgca
    10741 gcagctatcg cagtaccagt cgcattatta ggggatctgt cgccagaagc agcgattgcc
    10801 gttgcggtgc cagtcggatt gctcggaagt tacttgtatc agtttcgttt tttcatcaat
    10861 acttttttag gtaaatatac agacaaagct gtagaagatt tgaatacaaa agctatggcc
    10921 cgatcaatta ttctttatcc aacggttgcc tcatttttat tattcgttcc gcttgttttc
    10981 attgctttat atttaggggc gccattgatc gctgatgtaa tcaaagcttt agagggaaca
    11041 gtcgttatcc acattcttga agtagtaggt ggagggttag cggcaatcgg tatcgcgaca
    11101 actgtatacg ttatcggtcg aaaagattat ctggtctttt tcttccttgc ttattttatg
    11161 agtgttgttt tgaaatcttt agatattacg atggttactt atgccatttt tgggattatt
    11221 atcgctttga tctttgtcca gtcgcaaaaa gggaaacaag tacagcctgc cacaagcaat
    11281 aacaattcag catcttttga tgacgaagat gactatgatg acggatttta atttaggagg
    11341 aatgggaaat ggctgaaata atggataatc aaacaggtgc tcctgaagaa atcacgaaaa
    11401 aagatgtaac aaaagcctat ctgagatggc attttgcaaa cgaaattcct cactcttttg
    11461 aacgttactt ggcgccatca ctactatacg gaatgatgcc aatcttgaaa aaactttata
    11521 aagatgaaga ctctttaaaa gcagcatata aacggcagtt actgttcttc aacactcaat
    11581 taagctgggg tggcggtgtg attactggcc taatgtcttc tatggaacag caaagagcag
    11641 aagaagagta tgaagaaaaa gagatcttga tgcaggatga cttgatgtat aacacgaaag
    11701 cgggattgat gggggcacta gcaggaatcg gagatgcaat cgattcagga accgtccaat
    11761 atatttttat cgcaatcgcg gtaccttggg cacaacaggg aagtccttta ggcgcacttt
    11821 ttccttttat ctgctttggg ttgtatcagg ttttactagg ggtatttttc gcaagacaag
    11881 ctttcactat gggccgtaat gctacaggcc taatgcaaaa cactggtgtt cagacagcga
    11941 ttgaaatgct atcagtttta gggttgttta tgatgggggt gcttgcggga aactatgtaa
    12001 aagtagaatc ttcattgaaa tttgcgatat ccggacgtga gtttgttatc caggaaattc
    12061 ttgatcaaat cgtgccaggg atattacctt tagctgtcgt aatgggtgtg tacttgttct
    12121 atacgaaaaa aggattgaaa gtcacacaag cattattatg gctgactgga atattaattg
    12181 tgctagcagg aatcggtatt ttataaaaag aagggaacgt gaataagatg ggtgtagtca
    12241 atttagcgcg tgtggatgag cgtttgatcc atgggcaagt aatggtaacg ttatctcaaa
    12301 aaagtggagt gaattccatt tttgttgtag acgaagttgt agcaaaagat aaatttatga
    12361 gagatcttta caaaaatgca ggaagccgta caggacaaaa aacaattgtc attacaccag
    12421 agaaagctaa attctattgg gatgaatatc aatttaaaga atacaattgt attttgatcg
    12481 caaaaacagt ccaaacgatt tacgacttag taaaacacgg aatccccatg gaagaattga
    12541 atattggagg gattgctcaa aaaaatcctg atacagatct tttagtgacg aaatctgttt
    12601 atttaaataa atcagatgct gaaaaattga aagaaatgaa tgaacaatat ggcgtaaaga
    12661 atatttattt tcaagccact ccatctgcac ctaaaacagg attaaaagat gtattgaaca
    12721 agtttgattt gtcataaaca aatgcttaga aaaaagataa agagggcgaa aaaatgaaga
    12781 agaacgagct gtttcgtgat tgggaatttc gttatcgcta tatttaccgt aagcgccgta
    12841 caaaaaagag taaacagcgt tttctttcag cattagtatc agatatttac agtatgagaa
    12901 cagatgtgac agtcattgct tacgatacgc tagcctaccg atcaaaaaat atttatgttg
    12961 gagatatcga aaaagcagag aaagttatct gtacttatta cgatacaccc gttcatgcat
    13021 taggaagtta tttcatgttt gattggaaag accaaaggaa aaaaacaatc tactctatat
    13081 tattgtcctt cattttgttg ttcagtttag gttggtgggg aatgatgata tataacaaaa
    13141 atcctcatca cgtattcgat ctgctatccg tataaacagg tatcactgta ctagcatttg
    13201 ggagctactt tttcttattg ggaaaagcag ctcgcggatg gcctagcaga caaactttta
    13261 ttcgaaatac ttcatccata ttaacgatgc tagaaatgat ccgtacgatt gatgatccga
    13321 gtgtagcgta tgcgtttgtt gatgaaggct gttatggaga aaagggctta gacagcgtga
    13381 gatcgggtat gaaaaaagag gctattcttt tttatctaga ctctgttggg gcagatacac
    13441 cgctacagtt ttcgggaaat tatttttcaa ataaagaaca gtggctgaaa caagttgata
    13501 agttgaaaga aaaaaacgtc aattatattt ttagtgcaag aaaaaagcaa gcgcagttct
    13561 tctatttgac caaaacagat ttacgaggga aaacatttaa ttggcaaaat gccaatcaga
    13621 ttattgctct ttttcgataa gtaaaaagtg caaaacaagg agtgagaaga ctaatgttgg
    13681 aaatcatgat cgttacacat ggtaagttga gcgaaggact gaaagatgcg gcggaagtaa
    13741 ttgtcgggac atcgagccat attgaaacaa tgtgcttaga gtctggggag gacatccaaa
    13801 cattcggaga gaaggtcaaa gaaaaattaa ctatgatcaa cacagcagaa cccatgctag
    13861 tatttgttga tctaattagc gcaagtccgt acaatcaagc gctcttggca gtcaatgaac
    13921 tgactgaaga aaagaaagca gccacttatg tcataggggg cgtgaatctg ccaatgttgt
    13981 tagaagcaat caatcatcag atgcttcaca caccgataga agaagcggtg gaacaaattg
    14041 tcaaacaagg aaaagataat ttaactgttt ggcatgcttc agcggaagaa aaaaaattag
    14101 atgaggacga tttttagaag gaaggctgaa aagatgaagt ggggatttag atggtatggt
    14161 gaaaaagggg attcgatccc attaaaatat gttcgccaaa taccgggaat aaatgggata
    14221 attggaactt tgctgactaa aatgcctgga gatgtttggg agatttcaga aattgaggag
    14281 ttgaaagcat ctgttgagag ccaaggactg gaactgcttg gaattgaaag tgtagcgatc
    14341 cacgatgcaa tcaaggcagg gacagatgaa cgagattact atattgataa ctatatacga
    14401 acaatcaaaa acttgtctcg atgtggggtt cgattgattt gttatagctt caaaccaatt
    14461 tttggatggg cgaaaactga tttgtttaat gaaaacaacg atggaagcta ttctctttta
    14521 tacgatcaga aaatcgtcga tcagatggag ccaggagaaa tgtatcaatt gatacacagc
    14581 cagtcaaagg gattcaaatt gccgggttgg gaagaagaac ggttgaaaaa attcgctcag
    14641 ctggaaaaac tgtatgaagg aatgaccgaa gaagaattat ttgaaaatct gacttatttt
    14701 ttagaaaaag tgattccagt gtgcgaaaaa gcagatgtaa aaatggctat ccatccagat
    14761 gatccgccat gggaactttt tggttttcct agagtgacga aaaatcttgg cgacttgaag
    14821 aaaattctaa atgcagtaga ttctccatat aatggactaa cgctgtgcac aggatcttta
    14881 ggggcaaatc cggaaaacga tttagttgaa atcatccatg aggtaggttc gcgcatccat
    14941 tttgtccact tcagaaacgt ggagttttta ggagaaagaa agttcaaaga aacggcacat
    15001 ccctctacag aaggctccct tgatatgtat catattatga aagcgttggt gcaagaaggg
    15061 ttcgacggtc ctatacgccc tgatcatggt agaaccgtgt ggggtgaagt ggctatgcct
    15121 ggatatggcc tgtatgatcg tgcaatggga ctcacttaca tgcaaggtct atatgaggcg
    15181 ataagtaagg aaaaaccact gggagagcat tatgaataaa atcaagctct tcatctttga
    15241 tatggatggc cttttattag aaactggacg tttagcttat cgtgcttatg taaaatcagc
    15301 acaaaagtat gattatgaaa tgcgtaagga agtctattat ttactaactg gccaaacaga
    15361 aatggcgata agagaacaaa tgggcatttt gtacggagaa gatgttccat atatcaaatg
    15421 gcgagaagca atcaatcaat ataaagaaaa aatcgtgaaa gaagataaga gagtatatac
    15481 gaaaaaagga gcagaagaaa ttttatcttt tgcaaaagaa agaggaatcc atacaatcgt
    15541 ggcttcgtct aatacacggg taaaagtaga aatgtattta aaaatggaaa atctatatgc
    15601 cttgtttgat caaatcattt caggggatga tgtaaaaaaa ggaaagccgg aaccagagat
    15661 ctttttgaaa gcttgttcaa aaatgaatat tcctccaagt gaagcacttg tatttgaaga
    15721 ctcgattgca gggatcgaag cagcaaggcg agcgggcatt ttgtcttttc tcatcaaaga
    15781 tcatttgata gggttgcccg atcataatgg gaagcataag ttgaaagtag atgtaagttt
    15841 atatgaatcc aataaagcag actatgaatt ttatgacctg catcaagcaa aacgattttt
    15901 agaaagtaaa gggttgtatc tctaataaag aggactattt tctagtcctc tttatttatt
    15961 gtcctgcacg actgagggaa cagatgaaaa agaagtaatt ttttattttg aagtatctac
    16021 cttatttgta gtgaacaatt tagaacaaac acaaaacgca tctactgtta tctaccagta
    16081 gatgcgttta aagagaagaa cgagaatcgt ccatatgagg gtttccactc cttatgcaga
    16141 tttttttaat tcttgcactg cttgttcgat cgtaacacct gtcgctacat gattggtatt
    16201 ttctttgtca aaaacgacaa atagattcaa atctgtatcc aaggttactc gatattgtat
    16261 atctttgtta tagaatgtca tagccattct cctttcttta aatacttgct gaaaggcaac
    16321 gaacgcgttg ataacgaaat tccatttata agtataccac caattgaaag gttttcatag
    16381 ttaagaaatt ttaaagtcaa aagttgcaga taaaagcaaa taaacaagaa aacaaaaagt
    16441 gaatcaatcg cggttattca
//
LOCUS       DAZKNO010000051        16394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000051 DAZKNO010000000
VERSION     DAZKNO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.051"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG8MY_001253"
     CDS             complement(296..1024)
                     /locus_tag="ACG8MY_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430462.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG8MY_001254"
     CDS             complement(1002..2576)
                     /locus_tag="ACG8MY_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0430463.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG8MY_001255"
     CDS             2805..3743
                     /locus_tag="ACG8MY_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0430464.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG8MY_001256"
     CDS             3755..4666
                     /locus_tag="ACG8MY_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0430465.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG8MY_001257"
     CDS             4690..6162
                     /locus_tag="ACG8MY_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0430466.1"
                     /translation="MKKNMQKYLIGGVSVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6214..6825
                     /locus_tag="ACG8MY_001258"
     CDS             6214..6825
                     /locus_tag="ACG8MY_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0430467.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6855..8768
                     /locus_tag="ACG8MY_001259"
     CDS             6855..8768
                     /locus_tag="ACG8MY_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFQ0430468.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8896..11877
                     /locus_tag="ACG8MY_001260"
     CDS             8896..11877
                     /locus_tag="ACG8MY_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFQ0430469.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11874..13535
                     /locus_tag="ACG8MY_001261"
     CDS             11874..13535
                     /locus_tag="ACG8MY_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFQ0430470.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13575..14507
                     /locus_tag="ACG8MY_001262"
     CDS             13575..14507
                     /locus_tag="ACG8MY_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFQ0430471.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14867..16393)
                     /gene="guaA"
                     /locus_tag="ACG8MY_001263"
     CDS             complement(14867..16393)
                     /gene="guaA"
                     /locus_tag="ACG8MY_001263"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0430472.1"
                     /translation="MVLDYGSQYNQLITRRIREFGVFSELMSHRITAEEVKAMNPKGI
                     ILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAE
                     LEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYG
                     IQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLS
                     GGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKER
                     FLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTET
                     AQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLG
                     IRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYD
                     YTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE
                     "
BASE COUNT         5238 a         2689 c         3301 g         5166 t
ORIGIN      
        1 atttaaataa ctacatccat ctattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt acaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgtttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgacga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttta ctaaatgaaa
     6181 agaatagact aagcagttag tggggggatt caaatgaacg aaaacaaatg gaccaaagga
     6241 tttaattggg tttttcaatt gttattacta aactttactt attttatact aatttgttta
     6301 ggatttggaa ttttaggttt tttccctgct acgatagctt tgattgctgg cgttgtgaat
     6361 cgaaaaaaat atggcttgca agagtctgtg aagttcatga gtaaaatcta taagaaaaat
     6421 tttgttagat ataatctata ttgctggtca gcaactttgt tgttggccgg caatattact
     6481 acgatctggc tttgttggca gcaaagcgca ccattgtttt atttattaag tacattatta
     6541 tcggtaatta cactgttcct agtctttgca atgttgaatt gggtcgtgat caaaagtctt
     6601 gggttgtgga tgatgggaga ccgactgatt gactatttgc ttttcacagc aacgatgttg
     6661 ccaacggtag tcatgcaagg cttggtgctt ggaatttatt tccgaatatt gatgattttg
     6721 cctgtgattt ttctgtttgg cggaacagct gtaacggtaa tattgattag taaattaact
     6781 gaaagagcgt tagcaaagtt gccttgtcag atacaatcgc actaattttt tcacaaaaag
     6841 gagtatttct ttctatgaag ttaactgata aacaagtagc taatatagcc tttatccagc
     6901 aacttgagat tccattgaca caaaaagatg aagaacaaat agacttgatt tttactgaca
     6961 caaaggctaa tgtgcgacaa atcaagatta ctaaaaatga gcagcaacga atctgcttgt
     7021 cttattcaca tgaagacttg ctgtatcgag gattgtttat ctgccttcca cgttttttag
     7081 ctcaaggagg agagatgcca tacattgaag aaacctcgat cattgagacc agttattcat
     7141 tagatgcttc tcgtaatggc gtgatgaata aagagactct aaaaaaattc atttcttact
     7201 tagcgttgat ggggtacaac acattgtata tttacacaga agatacctat gaagtagaag
     7261 accttccata ttttggttac cttcgcggag cgtatacaaa agaagaagtg aatgaaattg
     7321 ttgaatttgc ttctggtttt ggcatagaag tagttccatc gatccagacc cttgctcact
     7381 tgacccaatt tttgaagtgg tatgctcaac aagaactgat ggatgatcaa aatactctac
     7441 tgatcggtga agaaaaggtt tatgcggcaa tcgaaaagat gattattgcc tgcaaagaaa
     7501 tgtatcagac agagcgtatt catttgggaa tggatgaagc ctatcaagca ggtctgggac
     7561 gatatttaag cctgtatggg tattgtgatc ggacaacttt aatgattcaa catatggacc
     7621 gtgtgttatc aattgttgat aaatataaga tgaaaccaat gatttggagt gatttcattt
     7681 ataagctatt agatgaaacc ggtaaagata ggtattacaa tcctgatttt aaactcaaag
     7741 cattagcagc taaaggatta ccagcaggat taacctatgt tcattgggac tatggttcag
     7801 atgaagtttc ctcctatgag aaagtaattg ataaccattt gaaattttgt gacattgatc
     7861 attacgtttt agctagtggt gcccatatct gggggagact agccccgaat catgggaaaa
     7921 cagaagcaat tgtttctgca gctgtaaaag cttctaaaaa taaaggtgta cgttctgtga
     7981 tgttaactac ttggggagat gaagggcagg aaacagagca ttggcattcc ttaattgggg
     8041 cattaaaatt agcagattat gtctattctg aagaagagtg gaatgatttt gaggaaatct
     8101 acgatcgttt attaggaaca ggtagttatc aattaatgtc cgaattacga tatttcgatg
     8161 aagtggatac cattacaaat aacaacttac aaatggcttc cgtgagtaaa ctgctacttt
     8221 ggcaagatcc gttagttggt atatatgact ttcatgtagc cgctcataac aaaattgcca
     8281 ctagaacaat tggtacttat tatcatgagt tagctcagaa attagatatg aataaaaatt
     8341 ggaatccaaa acaaccaatt ttaacaacta ttcgtcaacg atatatactt ttagctgaaa
     8401 ttttagcaca aaaggcagaa ctaggcatct atttacaaca ctgctgtctg gtagaagacc
     8461 ttgcaggatt aatggaatta gcgaatcggt gtgaagcact agccaatcga gtaaatgaat
     8521 tggcatctat tcatgaaata atttggtatc aaacatacaa aacctatggt tgggaagtat
     8581 tagaacaacg atatgctgca ttagctcatc gtctgaaaac cgttgcgcgt cgtattaaaa
     8641 atcatcttca ggggcaagat gccctagagg agcttttgga agaacgatta ccgttttgtc
     8701 aatatcaagt gccgcttgaa atttctggct ttaattatcg gcaaagctcg gtgtcaggct
     8761 acaattaacg aaggaatctt cccgttgaac ggaccacttg ctcatctaat gggaaagtgg
     8821 ttcgttttct attcataagt agcttaccac taactctttt attaagaaga gaaaaaaagt
     8881 aggaagtgag aaaatatgtt atcggactat tatcaaaatc tgtcagttat aagtgtcaat
     8941 acagaaccat tacgtaatta ttatattcct tatgcaagta aagaagaagc aaaacaagag
     9001 ttagaaaggg aaaaaacgag ccagtttatc gatctcaatg gtacatggga ttttcagtat
     9061 tatccttctg tccaacagtt accggaaaat ttttttcaag aggcctttac tgaaagttat
     9121 gatagaattt ctgttcctgg agtttggcaa acagagggat ttgacttacc tcagtatatc
     9181 aatgtatctt atccaattcc ttttgatccg ccttatgtac ctaatgatac cccttgtggt
     9241 gtatatcggc gtcagtttca aattgagcaa acagatgcac gcttttactt aaatctagaa
     9301 ggagcagata gtaattgttt tgtttggata aatagaaact ttgtaggcta tgaccaagtg
     9361 agtcatagta catctgaatt tgatgtgacc aagtatttac aggttggggt taatgaaatt
     9421 caaattctag tgatgaagtg gtgtgatggt acctactttg aagatcagga taagtggcga
     9481 atgagcggtc tatttcgtcc agtgtatctt cttcaacgac caactatgca tctgacaagt
     9541 tatcaactac ggacgttgac tgatcctaaa aaaggaagcg gaacattaat ggtttctttt
     9601 caacgattta ctcctattga agtatcgtta cctatttctt acgaattatt ccatcctgat
     9661 ggacgtctca tagcgaaagg ggagtgttac tcggaacaac aaataaattt ggatgttgaa
     9721 gattgccttt tttggacagc agaaacgcct aaattatata cattactttt aacatgtaat
     9781 ggtgaatatt ttaaagaaaa agttggcttt cgagcactga cgatcaagaa tcgtaagtta
     9841 ttgctgaacg gaacacctat taaattatat ggagtcaact atcatgacgt gaatcagcaa
     9901 ggcacgccag tgatgacccg agagcagtta cgtgcagatt tgttattaat gaaaaaatta
     9961 aatgtgaata ctgtacgaac ggcacattac cctaaatcac cagattttta tgaattggcg
    10021 gatgagattg gtctgtatgt aatcaatgaa gcagatttgg agtgtcatgg tgtggttaat
    10081 ctatatgata ctgaagcgaa ttatcaacta attgctaatg atccgacatt cgaaaagccg
    10141 attatggacc gagtgatccg ttctattctt ccttatcaaa atcgaagctc tatcattatg
    10201 tggtctatgg ggaatgaatc tggttatgga gtaaattttg aaaaagctca agctttagca
    10261 cggaaattgg atgattcacg gttgattcat aatgaacgct cgattgaacc agttattgac
    10321 caagtgaatg attttagtaa cttggatgtt attagtcaaa tgtatccccc actggaagat
    10381 gttcatcgtc attgtcaaag aagtgatact gaaaaacctt tttttatgtg tgaatatagc
    10441 cacgctatgg gcaacggtcc tggagatctt aaaacgtact tcgatttgat tcaacagtat
    10501 gattcctttc ttggcggttg tgtctgggag tggactgatc atgcaatcaa tagaggaact
    10561 ttagaaacgc catcttattt atacggtggt gattttggtg aaaaatatca cgacggcaat
    10621 ttttgcgtag atggtttatt gaccccagat cggcaattaa cagtaggtgc attggagtac
    10681 cgtaatattc attcgcctat tcaactgatt gctgtagatg aggagctgca agaagtaacc
    10741 ttgagcaata taactgcttt tacagccggt tcagcattta acctttctct tgaatggtat
    10801 atagatggaa aaaaaacagt ttatgaagta atgaatcctt tcatacttga gcctggcaaa
    10861 agttgttcat tgcccatcac agtacctaca acgattcatg gtgctcatac ggttgctgtt
    10921 tcgctatctg tttataaaaa tgatgagctt tatggacagc aacagctctt tcttaaaaaa
    10981 gaaaaaatga gattgccaag aactgctcat cataatgatg caccacaaat ttcagtaaaa
    11041 ggcacagagc tgataatcaa aggagaatcc tttatttata tatatcaatt agcaaaagct
    11101 gcgttcactt ctgtaaagat caatcaaaag gaacttttgg aacgtcctac tcaattttca
    11161 atttggcgtg caccaacgga taatgatcgt aacttagtaa acaaatggaa agcagctgga
    11221 tatgctgaaa tgtctttaga aatcagaaaa aaagactgga aattcgtaga tgatcatctc
    11281 ctgatcgaaa cgtcttatcg aggagttatt ccaggattac agccaatttt tacaatgcac
    11341 atacagttga aaataaatgg tgcaggcgta atcaaccaaa aagtatcagt ggagaaaaat
    11401 ccgatttttc cagatttccc tcgctttggg atggactggc cgttgaatga tactttttcg
    11461 aaggtttctt attgtggcta tgggccgtat gaaagttatc ctgataagca ctatgctgcg
    11521 atgtttggta tttatgagat ggagattgag gaatttgatc agccatatat caaaccacaa
    11581 gaatatggta accgctggca atgtgattgg gtatcaatca aaaatcatca aggatctacc
    11641 atgacggttt atggaaactc taatttttct gctctgcctt atagtcaagc tcaattaacc
    11701 gaaaagtcac accattatga attaataaaa gaaaatagaa cttggttcaa tttaagttat
    11761 aaacaaaatg gtatcggctc acacagttgc ggtccttatt tagaagatcg tttccgtttt
    11821 atggaagata cttttgactg gaatatagtg attactttca aaggagaaaa tagatgaaag
    11881 aaacaaagat caatcaagtc aaaaaaaaat tatctcgtta tcaagaagtt gtttatcgaa
    11941 accattgctt aaaatggtca aaaaataagc tgaatgttcg ttttaatgga ttattttatc
    12001 gtttcgaaca gttagaagaa gtctggaaac cggtaacgaa tttccaatca tgtcctgtac
    12061 agctcataaa tgatccaaaa gaaacatacg atttatgtat aacacacctt tataaccagc
    12121 atttgaaaaa tgacggattt tatttaaagg ttactaatga caatcaactt acgattgaat
    12181 cagcaaacct tcgagggttt caatatgcta tggaagcctt attagagagt tttttttcgg
    12241 taggagatca attattatta ccggaattga ttctgaaaca tgaaccatca gtgaaaattc
    12301 gaggaatcat tgaagggttt tatggtatcc cctggacaca tgaaatgcgc ctctctcttt
    12361 tttcatatat gaaagataac caattgaata cttttatgta tgcccctaaa gatgatgaat
    12421 tattacgtaa aaaatggcgt gaactctacg atgcgcaaga attagacaaa tttaaggagc
    12481 ttttatctgc tgctgaggct agcaatatcg atttttggta cttaattagt cctggaaatg
    12541 atattgatat tacgtgtgaa gaagacattc aagttcttct gaaaaagtta gaacaactga
    12601 ttgagcttgg cgtatttcaa tttggtctat taatggatga tattgactat caattaaaag
    12661 gtgctgctaa acgccgtttt cgagagccag cattcgctca tgcctattta gtgaatcgag
    12721 tagaggaata tcttagcact cggttgtcca atcatgaatt agtcatttgt cctacagaat
    12781 atgataatcg tcatggttct cgctatttag caacgttaag tcaagaaata ccagaacaat
    12841 taccgttctt ttggactggc ccaagcacgt tagcggcgtc aatttctaca gaagagctgc
    12901 aggaaatggc ttctgtctat caacggccga tgctgatttg ggataatatt ccggtaaatg
    12961 attatttaga ggataaagaa ctattgttca tgagtccata tgagaatcga acaccgaatc
    13021 tttctaagga gagataccag gttacaggag tagtttcgaa tccaatggct caattagaag
    13081 cttcaaagtt tacgatcaat agtatggcta attacttatg gaactgtgag cgctttgatc
    13141 ctttggaaac ttggacgtca gtcgttgaaa aagtagttgg aagtaaacta caaccagctt
    13201 atcttacctt aaccaatgct tttccaaatc attacacaag cagtctgctt tcagatgaag
    13261 aattactgct agccaaagac ccagaatggg tcacaaaaga aatggctgct ttagtccaag
    13321 cagtaaaatg gatccagcaa gaacaacata catctagatt gagaacagaa ttggagcctt
    13381 ggttacaagc aatcacagaa agttggactt tctggcaaga atggcaatta aaaaaagatc
    13441 caaaagaagc agaagaatgg ttggctaaga aaaaaacaca ccgcatagga cgtgatttag
    13501 taactgccca tttagagcaa attatcaaaa gttagatatc ttaaaaaata aaaagcaaga
    13561 aaaaggagaa caaaatggtc aagagattaa ttagtgcaaa ctacagtgat gtcaagaaaa
    13621 tgacgtcaaa agagttgaag caatccatca aagcgagtga aggacgggtg attgtttgtg
    13681 aaacggttgc taatactctt ccacaatctg gagaaattac gaatgcagaa gttgcgtcag
    13741 catttggtgc agatttgatt cttttaaatg gtttcgattg ttatcgtcct gcagtttttg
    13801 gactaccaaa tgtgacgatt gagaaggttt ttgatctaca gcaaacgatt gaaaacccga
    13861 ttcctaaatt aaaagaacta gtcggtcgtc ctgtgggaat taatttagaa cccattgccc
    13921 ctaaaacaaa tatgatgagt gagaaagtag aaatcagcca aggaagagca agctctgttg
    13981 aaacgataca aaaagcagaa gaactaggag tcgattttat ttgctttacc ggtaatccag
    14041 gcactggagt aaccaattta gaaattacta aatccgtaaa aatggcaaaa aagtattttt
    14101 cgggattgat tattgctggg aaaatgcata gcgccggctc agaagagcca gttgtgaata
    14161 aagaggcgat tatttcttat gcggaagcag gcgcagatat tttactagtt ccttcagtag
    14221 ggtctatccc aggcttttta gagcaagacc taattgaagc ggttcggttg gcaaaaaaat
    14281 atgatttgtt aacgatgtct gcaatcggta ctagtcagga aagtgcccga ccagaaacat
    14341 tgcggcaatt agccattatg aataaaagtt gtggtgttga tttacaacat attggagatg
    14401 ctggatacgg gggattggca ccagcagaaa atattttcga aacctcggtt gcaattagag
    14461 gaatgcgaca taccatcaac tgcgttagcc gctcgatcaa tcgctaacaa aattaaataa
    14521 aaaatggtta aattgattat ttaagtggga acatacttga gatatcagct aacttctgtc
    14581 attttaaaaa aagggactgt gacagaagtg aaaatagaca aaaaatatcc gaacaagaga
    14641 ctaggatagc agaaaaacta tcgttttgca acgaggacca actggaacgt aggagcaatt
    14701 ctcttattgt tcggatattt cgcttgttca aggacttatg tctgtagtgc ccttttttgg
    14761 taggaaaaat ggtagggaac catatcattt ttaatgaaaa tcaagcattt caaaagttga
    14821 aaaacgttga tttatcggca ttccccatcc atattatatt tacgtcttat tcccactcca
    14881 ctgttgcagg tggtttgctg gtaatatcat acacgatacg gttaacgtgt gcgacttcat
    14941 ttacgatacg gacactgatt ttttgcaaga cttcccaagg gatacgggca aagtcagcgg
    15001 tcatgccgtc aatagaagtg acagcacgga tacctacagt gtagtcatac gtacggccat
    15061 ctcccataac cccaacagag cgaatgcctg gtaggacggt gaagtattgc cagatgtcgc
    15121 gatctaaacc ggcattggcg atttcttcgc gcaagatagc gtctgattca cggacgattt
    15181 ctaatttttc ttctgtcaat tcaccaagga cacgaatacc taagcctggt ccagggaatg
    15241 gttggcgcca aacgatgctg tcaggcatgc ctaattgggt acctaattca cgtacttcgt
    15301 ctttgaacaa tgtgttcaat ggctcgatca gcttgaattg catgtcttca ggtaatccac
    15361 ctacattgtg atgtgatttg atggtttggg cagtttcagt tcctgattcg atcacatcgg
    15421 tataaagggt tccttgtgca aggaaagaaa cgccttcttc accggcaagt ttggttgctt
    15481 catcatcgaa gacatataca aattcatttc caatgatttt acgtttttct tctggatcag
    15541 acacgccagc aagttttgat aagaaacgtt ctttggcatc gacttttata atgttcaagc
    15601 caaattttcc gccaagactt tccataactt gttctgcttc acctttacgc aacaatacgt
    15661 gatcaacgaa gatacatgtt aattgatcgc cgatcgcttt ttgtaaaaga acaccaacaa
    15721 cagaggaatc tactccgcct gaaaggccta ataaaacttt tttgtcaccg acttgttcac
    15781 gaatttttgc gatctccatg tcgatgaagt tatccattgt ccagtcacca gtacattggc
    15841 agacatctag tgtaaaatgg cgcaacaagt catttccata ttcggaatgg cgaacttctg
    15901 catggaattg gattccgtac attttgcgtt ctgcattttg gatcgatgcg atggggcaat
    15961 ctttgctggt agcaacgaca tcgaaatcag tcggtgcttg ggtaactagg tcaccatgac
    16021 tcatccaaac ggtttgtttt tcaggagtgt tttggaaaag ggccgcttct gcagaagtga
    16081 tttccaattc tgcttttccg tattcacggt ttttggctgg tttaactttt ccgcctaatt
    16141 tataagtaat caactgcata ccgtaacaga ttcctaaaac tgggatcccc aaatcaaaaa
    16201 tcttttcatc aataccaaag gccttctcat cataaacact gtttggtccg ccagaaagga
    16261 tgattccttt agggttcatt gcctttactt cttctgcggt aatgcggtgg ctcatcaact
    16321 ctgagaaaac accgaactca cgaatacggc gagtgatcag ttggttatac tgactaccgt
    16381 aatcaagtac gata
//
LOCUS       DAZKNO010000052        15924 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000052 DAZKNO010000000
VERSION     DAZKNO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.052"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..221
                     /locus_tag="ACG8MY_001264"
     CDS             <1..221
                     /locus_tag="ACG8MY_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0430473.1"
                     /translation="STGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDMLR
                     SMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            363..2114
                     /locus_tag="ACG8MY_001265"
     CDS             363..2114
                     /locus_tag="ACG8MY_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0430474.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            2114..3898
                     /locus_tag="ACG8MY_001266"
     CDS             2114..3898
                     /locus_tag="ACG8MY_001266"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430475.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            4233..5084
                     /gene="licT"
                     /locus_tag="ACG8MY_001267"
     CDS             4233..5084
                     /gene="licT"
                     /locus_tag="ACG8MY_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFQ0430476.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            5181..7067
                     /locus_tag="ACG8MY_001268"
     CDS             5181..7067
                     /locus_tag="ACG8MY_001268"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0430477.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(7108..8010)
                     /locus_tag="ACG8MY_001269"
     CDS             complement(7108..8010)
                     /locus_tag="ACG8MY_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0430478.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            8240..9643
                     /locus_tag="ACG8MY_001270"
     CDS             8240..9643
                     /locus_tag="ACG8MY_001270"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0430479.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            9643..11076
                     /locus_tag="ACG8MY_001271"
     CDS             9643..11076
                     /locus_tag="ACG8MY_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0430480.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            11221..12147
                     /gene="trxB"
                     /locus_tag="ACG8MY_001272"
     CDS             11221..12147
                     /gene="trxB"
                     /locus_tag="ACG8MY_001272"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0430481.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            12361..14088
                     /locus_tag="ACG8MY_001273"
     CDS             12361..14088
                     /locus_tag="ACG8MY_001273"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0430482.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            14104..14454
                     /locus_tag="ACG8MY_001274"
     CDS             14104..14454
                     /locus_tag="ACG8MY_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0430483.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14548..15408)
                     /locus_tag="ACG8MY_001275"
     CDS             complement(14548..15408)
                     /locus_tag="ACG8MY_001275"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0430484.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            15500..>15924
                     /locus_tag="ACG8MY_001276"
     CDS             15500..>15924
                     /locus_tag="ACG8MY_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0430485.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENC"
BASE COUNT         5078 a         2773 c         3538 g         4535 t
ORIGIN      
        1 tatcaacagg tattgtcgta cttattgcta ttattgcttt gctaatcggc gcagtaggtg
       61 gctttttcct agcgcgtaaa tatatgaaag actatttaga aaaaaatcct cccgttaatg
      121 aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa accatctgag aagaaaattc
      181 gccagatgat gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc caaagacttt
      241 ttgcgggctg attttttcag tccgtttttt atttcgtaat ttgttagact attcagacta
      301 ttcagcaaaa aagacaagga cagacaatcc tatgctcttg ataaagacaa aggaggagta
      361 aaatgtcgat ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa cattatttaa
      421 ttgggatttt ctccctattc gcagtggcct tggtacagtt agttcctcca aaagtgattg
      481 ggatcatcat tgatgagatc gccgataaga atttatcaat aaagatcatt tcttggtgga
      541 tcttactatt gatccttgca gctttcttac aatatctttt tcgttatatc tggcggacga
      601 atatttgggg aagtgcagct cggttggaga aagaacttcg gcagcagcta ttcaaacatt
      661 ttacagaaat ggacaatctc ttttaccaaa aatatcggac tggagatttg atggctcatg
      721 caacgaatga tttgaatgca atccagaatg tagcaggagc tggaatcttg acctttgcag
      781 attcagtgat tacgggcgga acaacgatca ttgcgatgat tttgtttatt gattggcgct
      841 taacactgat tgcgctgatt cctctgcctt tgctagccgt tacttcacga gtgctggggt
      901 caaaactcca cgatgctttt cgtgattcgc aagaagcttt ttctagtatc aatgataaga
      961 cacaagaaag tatcactggg atcaaagtca ttaaaacatt tggacaagaa aaagaagatc
     1021 tcgcggattt tgaagaaaag attgaagatg caattattaa aaacaaacga gtcaattttt
     1081 tagactcctt atttgatcca ttcattacct tgattatcgg tctgtcttat gttgcaacaa
     1141 ttattttagg tggaaagtat gtcttggaag gcacgatctc gattgggcag atggtctcat
     1201 ttattagtta tatcggtatg cttgtttggc cgatgtttgc gattggccga ttgttcaacg
     1261 tattggaaag aggaaatgct tcttatgatc gcgttaatga acttttgcat gaaaagacgc
     1321 atatcgtgga acaactgggt gcagtgcaga ccccggcaaa aggcgagctg aatgtagcta
     1381 tttcctcttt taactatccg aaagaagaat caccagcatt agaacaaatc aaatttgcga
     1441 tccaagaagg agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa acgacaatca
     1501 tcaaattact gatgcgtgaa tacgatgatt atcaagggaa aatccaattt ggcggacgtt
     1561 cgatcaaaga atattcattg aatgctttat taggatcaat cggttatgtg cctcaggatc
     1621 acttcctgtt ttcgatgacc attcgagata atatccgttt ttctaatcca aaactttcag
     1681 aagaagaagt agagcatgcg gcttctttag catttatcaa caatgaaatc aaggaattgc
     1741 caaaaggtta tgatacctta gttggagaac gtggagtctc tttatctggc ggacagaaac
     1801 aaaggatttc gattgctcga gcattgatca aaaatcctga attgctgatc ttagatgatg
     1861 cactttctgc agtagatgcc aaaacagaag aagctatctt gcataattta aaagaaataa
     1921 gaaaagagaa aacaaccatc attgcagctc accgtttaag cagcgtgatg catgcaaaag
     1981 aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac gagctgcttg
     2041 cattaggtgg ctggtatgaa cgaatgtggg aaaaacagca attagaagcg aagatagaag
     2101 gaggcgaggc ctgatggatg aaaattatca atcggaatgg acaaaatcga ttccacttaa
     2161 agaacagaag aagatcatca aacgactgat gaagttcgcc aaacctttta gaagaacgtt
     2221 catcgttgct atcttatttg cgtttgccct gtctgtgatc aatgtcctat tgccaaggat
     2281 catccaaact tttatggatg atcatctggc taaacagtca gctacgacac aagttatttt
     2341 gttttttgct ggtgtgtact tgtttggagt catagtcaaa agtatcattt ggtttttcca
     2401 atggtatttg tattccatgg cttcattgaa gacctatcag tatgttcggg taagattatt
     2461 tgaaaagctg catacactag gtatgcgtta ttttgaccag acaccggcag gatcgaccgt
     2521 ttctcgagtg acgaatgaca cagaaacgct atttgaattt tggtatgtct ttttgatggt
     2581 attgactggg atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa tcaatggaga
     2641 aattgccttg tggactttgg tgttcttgcc gattttagga atcgtcatct ggtattacca
     2701 gaaattcagt tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc tgaacacgaa
     2761 attgaatgaa tcgatttccg gcatgcgtat tatccagcag tttcgccaag aagctcgttt
     2821 agctaaagaa tttgaacaag tgaacaacga gtatttagac acgcgctatg cgatgatcaa
     2881 aacgaattcg attctgttaa gtccgatcat caattttctc tatgcattgg cgattgcgtt
     2941 ggcgttgact ttgtttggga tcgatgcgtt gcattctccc gtagaagttg gactgatcta
     3001 tgcatttacg acctatgtcc agggattttt taatccaatg tctcaaatga tggacttttt
     3061 gagcaccttt acagatggga tggttgccgg tagtcgtatt ttgaaaatca tggatacaga
     3121 agaatatgca ccgcaacaaa atcctggagc agatcaagcg attacttcag gtaagatcga
     3181 gtttcgcaat gtaagctttt cttatgatgg agagcacaat gtattgaaca atatctcttt
     3241 tgtcgctaat ccgggtgaga ccgttgcatt agtggggcac accggaagcg gaaaaagctc
     3301 gatcatcaac gtattgatgc ggttttacga attttatgaa ggccaaatct tgattgatga
     3361 tcatgacatt cgagaatatc caatggaaga gttacgtaag aaaatgggat tggtcttgca
     3421 agatgccttc ttgttttatg gagatattgc aggaaatatc cggatgatga atccagctat
     3481 cacagacgaa cagattcgcc atgcagctga gtttgttcaa gcaaacaagt tcatcgatga
     3541 attaccaaaa ggatatcact caaaagtgat cgaacacggt gccagctatt ctagtggaca
     3601 acgacagctg atcacttttg cgcgaacaat cgtgacagat cctaaaatac tagtattgga
     3661 tgaagcaaca gcaaatatcg atactgaaac cgaaggattg atccaagaag gattggcaaa
     3721 aatgcgacaa ggacggacaa cgattgcgat tgcccatcgg ctgtccacga ttcgggatgc
     3781 agaattgatt ttagttttag acaaaggtca tatagtagag cgtgggaatc atgaaacact
     3841 tctagcgcaa aatggtttgt atgcagatat gtacaaactg caaagcagtg gtgtgtaaaa
     3901 agaggttgtg acagtagcat agtagcattt tgtcctaagc gctagagtga aaataaaaca
     3961 gagcttccca gattcttcat ctgtgagcgt tagtggcgta gtcacccgat ttaaaaaaga
     4021 cgacgacagc tagtcaaaag tgcctaatag ccactaagaa agcgttgaca aaagcgtttg
     4081 ctttcgataa tataaagaaa acgatacaac ttggatagtg attgcctgat gcaagctaaa
     4141 cctgatgagt ctcccatggt tccatggacg gctttttggg tttttctttt tgttttgaat
     4201 acagccctat atatcatgga aaggagcaaa acatgctgat tgagaaagta ctgaataaca
     4261 acgtcgtaat aaccagggat gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg
     4321 cattcaaaaa gaaagtgggt gacccgattt ttgactcatg catcgacaaa aaatttcagt
     4381 tgacaaatga cgggctttca caacagttcc aagaattggc acaagcgatt cctcttgagt
     4441 acttagaaat ttcttgtgaa atcattgaat atacaaaaga ggctctacaa gtccagttga
     4501 atgattctgt gtatattgca ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa
     4561 tgggaatatt tgtaccaaat gtattgctgt atgatatcaa acaactattt cctaaagagt
     4621 atgccatcgg gaaaaaaaca gtgaagaaaa tctcggataa ataccacatc aagttatcag
     4681 aagatgaagc cggatttatc gcattgcatt tagtcaatgc acaaggcatt caaagccaac
     4741 aaaacaatac ctacctgttt actataacaa tacaggatat tctaaatatc gtccgctatt
     4801 actataagat tacatttgat gaagattcag tccatttttt tcgattcgtc acacacttac
     4861 gtttttttct gacacgcgtc ctagatggag aaaaatcaga agtgggagaa gtttctgagg
     4921 aattgtttca ggttattcaa aaaaaatata aggatacgtt tggttgcgtt gagaaaatag
     4981 cagacttttt agccgagacg ttcaactacc agatgtccac ggacgaaaaa atgtatttaa
     5041 cgatccatac tgcgcgttta gtgcatgaga atcgttgtaa ataaataaaa aaaggaggat
     5101 aaattaacaa gaacgtatga aataagggta gtgacattca gttggctaaa ccttccacta
     5161 atatattgga gggaatagaa atgggaaaat accaagaatt agcgagaaaa atcgtagaaa
     5221 atgtcggtgg gaaggaaaat atcaatggtt tgacacattg tatcacaagg cttcgtttca
     5281 aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa aaatatggac ggtattgtaa
     5341 cggtaatgcg agcgggaggg cagtatcaag ttgtcatcgg gaatcatgtg ccagtcgtgt
     5401 ttgaagaggt tataaaagca ggaaacctta cttttgatga ggctgtttca acagaaaaaa
     5461 tgagaccgtt tgatatgctt attgatggaa tatcgggctg tttccaacca ttccttgcta
     5521 tcttagctgc tggcgggatg attcgtggac tgacagcatt ccttgtcttc ttaggcgcgt
     5581 ttgaccgcgg ttctggcaca tttgttatgt ttgataatat cggtgattct gtattccaat
     5641 ttatgcctgt tattatcggt ttaacagctg cccgtaaatt taaagtaaat gaattcgtag
     5701 ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc gctggaagct ttgagtggtg
     5761 cagcagaagc accactaacg accatatttt ctggcactat ttttgaagca cctatttatc
     5821 aaacagtatt cggcattccc tggattgcgc gtaactacgc ttcttctgtc attcccatta
     5881 tctttattgt gttgttagct agccaaattc agaaaccaat taaaaagtta gtacctgaga
     5941 tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat tacaatgcct ttaggttttt
     6001 tactcgttgg accagtattc acattcgcaa ctgatatttt aatggctgga tttgaaacac
     6061 ttttagcact ttcgccagtt atttatggag caatcgtagg cttcttctgg caaattctgg
     6121 tgatgtttgg tctccattgg gcaatcgtac caatgggatt gatgcaattt tctgtgaatg
     6181 gttggcaaaa tattatgaca cctgttgcag tagtgtcttt tggacaaact gcagcactta
     6241 ccgcactcta ctttaagctt agaaatccaa aagacaaagc aattgcaata ccagccattg
     6301 tttctggtat tgtggggatt acagagccag ctatttatgg gtttacatta ccacgtaaaa
     6361 aaatatttat ttttacttgt gtcggtggtg cgttaggggg cgcgtatagt gggttaatga
     6421 atttaacttc ttggaatcaa ggtggactag gcatctttac aattcccaac tatattagac
     6481 cagatggtga tttaacagat gtgattaatg tattgattgg gatcgccatt gcgatggtgg
     6541 tatcctttgc gctgactttt ttcttttgga aggaggaagc aggtgaaaca gatgacagac
     6601 agaaaagatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc gctccactga
     6661 aagcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc ttgatctacc
     6721 ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg ttccccacga
     6781 aacatgcaat cggaatggtt tctgaaacag gtctggaatt gctgattcat gtcggattgg
     6841 atactgttca gttggaaggg aaatattttg aaagcttggt gcagcaagga gaaagagtga
     6901 aacaaggcga tctgttgctc cgcttcgaca aaaagaaaat cgaaaaggaa ggatacagtt
     6961 tagaaacacc tgtaatcgtc accaattaca tggattattt ggatattttg gaaaatcaag
     7021 gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg gttaggtgaa
     7081 agaatggaat agacttaact attcttttta gtccacttct ttattttcaa aattgaaagg
     7141 ctctttcatg atggctgagc tgatagggcg tttatcaaat ttttgtccta cagaaattag
     7201 atggactaaa ttctgttgat aattttctgc aaaaacacaa ctatataaca cgtctagaag
     7261 ataacaaatc gatgtattga tcgtaaaatt accaattttt gaatacagtc gttcgcgtgt
     7321 cgtaagtcgc aaagcggctg tactcattga ggatagcgtt gaatcaccga tactggtcaa
     7381 cgctaaaatt ggaacatgat tttgtttcaa tatttctgcg gtacgaagga tcacattgtt
     7441 ttcacccgta taagaaatca gaatcccgca catatcactc gtgcaattgt acgcttcata
     7501 gaacgattca ccaaaagtgg tgacaatcgt gacatccatt ttgatgcgct tcattttcag
     7561 catgaaatct tgagaaatca aagtattcga gttactggcg aaaatcacga ttttttttgc
     7621 ttgaagcatt agttgtttgg ctttttgcag ttggtcatga tgaatgagag aaagtgtatc
     7681 ttgaatcgtc atctgttcta aagaagccat tttatttgcg atcgtcataa taccgtctgt
     7741 ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc ccttcaagat aggctgtttc
     7801 agctaaaaaa gcattacgaa aactgaccca gccatcaaaa cccatttttt ttgctacacg
     7861 gatgagtgtg gacgggtgga cataacatcg ctcagcaatc tcttggatgg tcatttcgcc
     7921 aagtgctgga gaatcgctta agagaaactg aatcacttca ttttctgaag gagaaaagga
     7981 gatttctttg attttttcac gtaataacat gtagatacct tcttctagtt tttttacgta
     8041 aattataact tttttgaaaa tgatttgtaa acggatttat gttatatagc ttaataaagt
     8101 cattttttga caagatattt acaaaaatcc agtagaaaac actaactttt gtaaatgtta
     8161 ctggaaagaa acgaatttct attgtttgtt ttgtttacaa aaaagataat aaaagtgtaa
     8221 aaagcaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg gtggaggcag
     8281 cagttacacc cccgagttga tggaaggatt tatcaaaaga tatgaggaat taccgatacg
     8341 agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg tcagtcagat
     8401 gtctcagcgt atgtgggatg cttctccgta tgaagtcaag atctatgcaa ctttggatcg
     8461 ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg gtcaattaga
     8521 tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc aagaaacaaa
     8581 tggtgctggc gggatattca aagcttttcg taccgttcca gtggttttgg caatcgttga
     8641 agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc caagcgggat
     8701 ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgatcggat tatgcaatgt
     8761 gccagtaatg gcgacgatga cagaaccagc aatgcttggc aagaaaaaag aagagctgat
     8821 ctacaagttt gccggtctaa atcacttcca ttggcacaaa gtagcagata ataaaggaaa
     8881 tgaccgcaca gcagatttga ttgatcgttt atacgatgaa gacaatggat tgccgaaaaa
     8941 tatctttgac atcccgtttt tcaaagaaca attaaaacaa atgaacatga tcccttgtgg
     9001 ctaccaccgc tattactacc gtgaagaaga aatgttggaa catgctttag aagaatatca
     9061 aacgattggt acaagagcgc aacaagtgaa agaaacagaa gcagagctgt ttgaattata
     9121 caaagatcca gaattggatc ataaacctga acaattgcaa aaacgaggtg gtgcttatta
     9181 ttcagatgct gcctgtgaaa cgattgcttc gatctatgga aataaagaaa cacagatcgt
     9241 ggtgtctaca aagaatgatg gagctgttcc tgatctgccg gcagattgtg tagtagaagt
     9301 cactgcctat gttggcgcac aaggcgcacg aaatgtagcg tttggttccc ttcctgctgc
     9361 tgaaagaggc tggttgcaag taatgaagaa tatggaattg ctgacgatcg aagcagccgt
     9421 gactggtgat tatggaacag ctttacaagc gtttacgatc aatccgctta tacgatcagg
     9481 aaagacagca aaacggatca tggatgagtt gtttattgca catcagcagt atttaccgca
     9541 attcaaggaa acaatcagcc ggttaaaagc agaaggaatc gaagtccaag atgaactggc
     9601 aagagaattg tcaagcaata cacatgtaat ggaggaagta tgatgggatt cagaaaagat
     9661 tttttatggg gcggtgctac agcagccaat caatgcgagg gtggttatga tgaaggcgga
     9721 cgtgggttag cgaacgtaga tgttgtgcca atcggagaag accgttttcc tgtcattact
     9781 ggaaaaaaga aaatgtttga atttgataaa gatcattttt atccagcaaa aattgcgatc
     9841 gatatgtatc atcattataa ggaagatatt gctttgtttg cagaaatggg gttcaagact
     9901 tatcggatgt ccattgcttg gagtcgtatt tttccaatgg gcgatgaaaa tgagccaaat
     9961 gaatctggtt tgaaatttta tgaggatgtc tttaaagaat gtcaaaagta cgggatcgag
    10021 ccgcttgtga cgattacaca ttttgattgt ccgatgcatt tgatcaaaga atatggtgcg
    10081 tggcgttcta gagaactcgt tggtttttat gaaaatctgt gccgagtcat cttcaatcgc
    10141 tataaaggac ttgtcaaata ttggttgaca ttcaatgaga tcaatatgat ccttcatgca
    10201 ccattcatgg gcgcagggct gtattttgaa gaaggagaaa atgaagaaca aatcaaatat
    10261 caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc tcatgaagta
    10321 gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc atacacttgc
    10381 aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt cttcattgat
    10441 gtccagtcac gaggagaata tcccgcttat gcattgaaag aaatggaacg aaatggcatt
    10501 cagatcaaaa tggaacctgg ggatgccgca ttgctgaaag aacatactgt tgattttatc
    10561 tccttttctt attattcttc tcgagtttcg actactgaca acgaactctt ggaacagact
    10621 gcaggaaata tctttgcttc tgtcaaaaat ccatatctgg aagcaagtga atggggttgg
    10681 caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg ttatcaaaaa
    10741 ccactattta ttgtagaaaa cggcttaggt gcagtcgata cgcccgatga gaatggttat
    10801 gtggaagatg actatcgaat cgactatctg gcagcacata tccaggcaat gaaagataca
    10861 gtagaattag atggagtcga tctattaggc tatacgactt ggggatgtat tgatttagta
    10921 tcagctggta caggtgagat gaagaaaagg tacggattca tttatgtcga tcgtgacaac
    10981 gaaggaaatg gcacattagc ccgttcgaag aagaaatcat tcaactggta taaaaaagta
    11041 attgattcaa atggagaaaa tctttcgaat gattgatcaa tgatttgtaa aagtctattg
    11101 tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc agaacaatag
    11161 actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga gaatctacat
    11221 atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc tttatatgct
    11281 tcacgttcaa acttatccgt attgatgatc gaacaaggcg cccctggcgg ccaaatgaac
    11341 aatacggcag aaatcgaaaa ctatcctggt ttcgattcaa tcatgggtcc tgatctagct
    11401 tataagatgt atgaaggtgt ttcaagattt gggacagaaa atgcttatgg aatcgtgcaa
    11461 gatatcaagg atcacggcag ccacaaagaa gttatttgtg aagataaaac ttacgaagga
    11521 aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc tggcgaggaa
    11581 gaatttgctg gacgtggcgt ttcttactgt gcagtttgtg atggtgcgtt tttccgtaac
    11641 aaacgtctag tagtgatcgg aggaggagac tcagcagtag aagaagccat ctatttgaca
    11701 caatttgctt ctgaagtagt catcgtccac cgtagagatg aattacgtgc acaaaagatc
    11761 atccaagacc gtgcatttgc gaatgaaaaa atttccttta tgtgggatac agtcgttgac
    11821 gaaatcgttg ggaatgaaat ggtagtgaca ggtgtgaaag cacgtaatgt caaaaccggc
    11881 aatgagtttg atttggcagc agacggagta ttcatctatg ttggactgga tccattaact
    11941 gcaccattta gagaagcagg gttgacgaac gaagctggct ggattccaac agacaacgaa
    12001 atgaagacga atataccagg cgtttttgcc atcggagatg ttcgtgaaaa agatttgcga
    12061 caaatcacta ctgctgttgg tgatggtggg atcgctggac aacaagtgta taaatacttg
    12121 gaatctttag aagaaacaac tgcataagtt taaaaagagt ggaagaacgg tttcaattgt
    12181 tcttttactc ttttttttcg cgtgaaatct actgaatccg atttatgcga atacttgcaa
    12241 ccgtgcttga taaaagaatc ctttcttgat ttaagcgttt tttagacctc gtactttcat
    12301 atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg tgttattttc
    12361 atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga agaaaactta
    12421 aaaaaacaat taattgatct gggtgaagat cctgaaaaac ttgaagatgc tttttatgca
    12481 ccgttggaat tcggtactgc aggcatgcgg ggaatcttag gacctgggat caatcgaatg
    12541 aacatctata cggtaagaca agcaacagaa ggacttgcac gtttcatgga tacacaaggt
    12601 gaagaaacaa aacgccgagg tgtcgctatc gcttatgact cacgccatca atcaccagaa
    12661 tttgccatgg aagcagccaa aacattagca catcacaata tcccttctta tgtattcgaa
    12721 agtttacgtc caacaccaga gctttctttt gcagtccgtc atttgaagac gtttgcggga
    12781 atcatggtaa ctgcatccca caatccagca gcttacaacg gatataaagt ctacggagaa
    12841 gatgggggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt ccgtgaagtc
    12901 agcaatccgt taaaagttga agtattatct gacgacgaag ctgaacatag cggattgatc
    12961 acgatcatcg gagaagaagt ggatgccgct tatctagaag aaatcaaagc agtaacgatt
    13021 gatcatgaat tggtggaaac aatgggtaaa gatttgaaac ttgtgtacac accacttcac
    13081 ggaacaggaa aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga acaattcgta
    13141 ctagttccag aacaagctgt cgcagacccg gatttcacta cagtcaaatc accgaatcca
    13201 gaagaacact ctgctttcga gtatgcgatg cgacttggcg agaaagaagg cgctgactta
    13261 ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag ccgttcgttt gccaaatggc
    13321 tcctatgaag tattgacagg gaaccaaatc ggagccctgt ttgctcaata tattttggaa
    13381 gcacaccaat cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc aatcgtttct
    13441 agcgaattgc ctacagcaat tgcggaacac tatggggcta ccatgttcaa tgtattgaca
    13501 ggattcaaat tcatcgctga aaaaattcaa caatttgaag aagatcattc ccatactttt
    13561 atgttcggat tcgaagaaag ttacggttat ttagttaaac ctttcgttcg cgacaaagac
    13621 gcgattcaag cattgctctt attagcagaa gtagcagctt actataaaaa acaaggcaaa
    13681 acgttgtatg acggcttgca aacgattttt gaaacatatg gctactacgc agaaaaaacg
    13741 atttctgtga caatgagcgg acaagaaggt tctgcaaaaa tctctgcttt gatggaaaaa
    13801 ttccgtgaag aagcaccgac agaatttgcc ggagcaaaag tcatccaaac agaagacttc
    13861 aaagagttga cgagaacaga ccgtgaagga aacgtagaaa aactaacgac accgccatct
    13921 gacgtattga agtatacgct ggaagaaaat ggctggatcg ctatccgtcc ttcaggaact
    13981 gagccgaaaa tcaaatttta tatcggtgta aaaggaaact cttcagaaga cgcagctgaa
    14041 aaagtagccg cttatgaagc gattatcaaa aaaatgacag aagaataaaa aaacttgaga
    14101 ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga
    14161 ttgccagaca cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa aatgttgagt
    14221 gaccccactc gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa tgtgacagca
    14281 atcacccaag cagtagaaat ggaacaatct gctgtatcgc atcaactgcg tttattaaga
    14341 gaaaaccatg tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag tcttgatgat
    14401 tctcacgtac tggatatttt gaaccagacg ctcaaacatg tggaacatca ataacgatag
    14461 gtgcaaatag agacaaactg gactgtatca acgaatcttc ttcgctgaca cagtccagtt
    14521 tttttaagaa aaagacaaga aaaactgtta gtctgtttct tcggtggttt cagttccata
    14581 ataactatga tcttctaggt ctaaagcatt caaaataagt gaagctgttt cttcgataga
    14641 taaagaagcg acattgatta ctacgcagcc caatttttca taaagatcat tcgcaaaatc
    14701 gagttcagct cgtatttttt cgatatcgga ataagcagta tcaggattta atccgtaaga
    14761 gcgcatccgt tctttacgga tattgtttaa aatatctggg ttattcgtca gtcccacgat
    14821 ttttttagga tcgatctccc aaagctgttt gggaatatgt gcttgtggga tcaaaggtag
    14881 attggcaacc tttaaatttt tgttagctaa aaacaagctc aaaggcgttt tggaagttct
    14941 ggatacgcct agcaaaacga catctgcttc tagaaaccca cgaggatctt tcccgtcatc
    15001 gtattttaca gcaaattcca ttgctttgat ccgtttgaaa tagttctcat tcaataaatg
    15061 aagggcgcca ggttgccgaa tgggcttgat accagttaag cgctcgattt ctgatatcgg
    15121 cggagtcatc acatcaaaac taaataattg atgttcttca caaaaagaac gcgtcatctc
    15181 gatcaattct tgattgatca atgtgtgcag gacaagggct ttttctttta gtgcatcttg
    15241 cagtgcctgc atcaatgttt tctcatctgt cacaaatgtt ctgcggaata aattgaactc
    15301 aacggtagga tattgggcca tagtggcttg cgctaatttt gtcgcagttt ctccagcaga
    15361 gtcagagatg acaaaaaaag taacgatttc tgtagaatcg gaagacatgg ataaaacctc
    15421 acttgcattt tttttatgtt tattatacca tactctagaa tgtgtacttc gtaatcattt
    15481 tgatttggag gaataagtca tgaaaaaaca gacattgatt gaacaatcac tagaaacatt
    15541 gaaaaataac ggttttaaat atactaaaaa aagagaagca ttgttaactt atttagtgaa
    15601 gcgcaatcgc tacgtatccg ctaaagaagt ttttgactat atgaatcagg aattcaaagg
    15661 agtaagttac gatacgattt atcgaaatct ccatgatttt gcagaaatcg atttactgga
    15721 agaaaccgat ttaaatggag agatgaagtt tcgttttcat tgctgtcaag gggaaatcgg
    15781 ccatcaccat cactttatct gtacggtgtg cggaacaacg aaagaaatcc atatgtgtcc
    15841 aatgaattat ttcgaagaac agctcggtgg atgtacgatc gaagggcata gattcgagat
    15901 tttcggaaga tgtgaaaatt gtca
//
LOCUS       DAZKNO010000053        15865 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000053 DAZKNO010000000
VERSION     DAZKNO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.053"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..845
                     /locus_tag="ACG8MY_001277"
     CDS             93..845
                     /locus_tag="ACG8MY_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0430486.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            842..1756
                     /gene="pfkB"
                     /locus_tag="ACG8MY_001278"
     CDS             842..1756
                     /gene="pfkB"
                     /locus_tag="ACG8MY_001278"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0430487.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1774..3705
                     /locus_tag="ACG8MY_001279"
     CDS             1774..3705
                     /locus_tag="ACG8MY_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0430488.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3816..5375)
                     /locus_tag="ACG8MY_001280"
     CDS             complement(3816..5375)
                     /locus_tag="ACG8MY_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0430489.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5530..7764
                     /gene="pcrA"
                     /locus_tag="ACG8MY_001281"
     CDS             5530..7764
                     /gene="pcrA"
                     /locus_tag="ACG8MY_001281"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0430490.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7781..9817
                     /gene="ligA"
                     /locus_tag="ACG8MY_001282"
     CDS             7781..9817
                     /gene="ligA"
                     /locus_tag="ACG8MY_001282"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0430491.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9923..10228
                     /gene="gatC"
                     /locus_tag="ACG8MY_001283"
     CDS             9923..10228
                     /gene="gatC"
                     /locus_tag="ACG8MY_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0430492.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10228..11700
                     /gene="gatA"
                     /locus_tag="ACG8MY_001284"
     CDS             10228..11700
                     /gene="gatA"
                     /locus_tag="ACG8MY_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0430493.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11697..13127
                     /gene="gatB"
                     /locus_tag="ACG8MY_001285"
     CDS             11697..13127
                     /gene="gatB"
                     /locus_tag="ACG8MY_001285"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0430494.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13137..14192
                     /locus_tag="ACG8MY_001286"
     CDS             13137..14192
                     /locus_tag="ACG8MY_001286"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0430495.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14303..15676
                     /gene="rlmD"
                     /locus_tag="ACG8MY_001287"
     CDS             14303..15676
                     /gene="rlmD"
                     /locus_tag="ACG8MY_001287"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0430496.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15679..15807
                     /locus_tag="ACG8MY_001288"
     CDS             15679..15807
                     /locus_tag="ACG8MY_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0430497.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5396 a         2902 c         3479 g         4088 t
ORIGIN      
        1 actgatttat aatttttatg aacgtttttg actgatttat attgcgagta gaaaaaaaat
       61 atgctattat ttgtattgag gtgatcgtga aaatgcttac agaggaacgt agacaaaaaa
      121 tcctagactt gcttgaacag cagaaaatcg tgaaatcaca agatcttgta aaccttttta
      181 atgcttctga atcaacgata agacgtgatt tacaagagct ggagaatagt gggtttctag
      241 aaagagttca tggcggagct aagaaagagc aattattgaa ttttgagcag aatatgagtg
      301 aaaaatcact caaaaacgtt cacgaaaaac agcaaatcgc caaatcggca gctgaaacca
      361 ttaatgacga agatgtcatt tatttagacg ctggctctac tacgttagaa atgattcctt
      421 ttctgcaagg aaaacagatc acagtagtga caaattccgt tcaacatgcg gcaaatttag
      481 ttgatttgga gcttgctacc attattttag gtggaacgat aaagctggca actaatgctg
      541 ttttaggatc taatgcgata gaacaactaa aaaattttca ttttaataaa gcatttatgg
      601 gaatgaacgg tgcccatctt gaacaaggat tcacgactcc ggacccagaa gaggcagctg
      661 ttaaacgttt agccatgaaa aattccgaag agacatttgt attgattgat cataccaaat
      721 tcaaccagtt gtccttcacc tctgttgcac ctttggaagc agccacgatc ataaccgagc
      781 gttgtccatt agaattcgtt aatgaattcc gtgaaaagac aacaattaag gaggcaatac
      841 aatgatttac acagtaacac ttaatccatc aattgattat atcgtacgcg tagatgggtt
      901 aaaactaggc gatttgaacc gaatgaagga agacttcaag ttacctggag gaaaagggat
      961 caatgtttcc cgtattttga aacggatcga ttctcaatct acagcactag ggtttttagg
     1021 agggttcact ggcgaattta tttcgaagtg gcttgaaaaa gaacaaatcc agtctttgtt
     1081 cacaaccgta aaagaagata caagaatcaa tatcaaacta aaatcagata ctgaaactga
     1141 aattaacggt ttgggcccat ctatttcaga tgaagaaatc gatgagttga agcaagcatt
     1201 aggaacagta ggacaaggag atatcgtcgt attgtcagga agcgcgccgg ctagcctgcg
     1261 aaaaggattt tatcaagaac tgattgatat cattcgagaa aaaggtgcag atttcgtgat
     1321 tgatacaaca ggtgatgacc taaaaaatgc acttgttaag aaaccattgt tgatcaaacc
     1381 aaacaatcac gaattagctg agctttacaa tacaaccttc caatctgtag aagaaatcat
     1441 tccgtatggt aaaaaattgc tagctgaagg ggcacagaat gtgctgatct caatggctgg
     1501 agacggtgct ttgctcttca cggaacaagg aacttataaa acgaatgtat tagttcgccc
     1561 attgaaaaat tcagtaggtg caggggattc aatgattgcc ggctttattg gggcttacag
     1621 ccgtacgaaa gatccaatcg aagcctttaa atggggagtt gcttgcggaa gcgcaacagc
     1681 tttttctgat gacttggcag ctcgcgaatt gattgatgag ttgctgccag aagttgaaat
     1741 cacaaaaatt gattagtttc aaggaggata atcatgaata tcaaggattt aatggtcaaa
     1801 gaagcgatga tcatggattt gcaagcgact gataaaaaag gcgcaatcga tgaaatggtc
     1861 caaaaaatgt ttgatgccgg ccggatttcc gatatcgaaa catataaaga aggaattcta
     1921 gcccgcgaag cacaaacttc tacaggtctt ggtgatggaa tcgccatgcc tcacgcgaaa
     1981 aacagtgcag ttaaagaagc aacagtttta ttcgcaaaaa gccaaaaagg agtagattat
     2041 gaagccttag acggacagcc aacttatctg tttttcatga tcgcagctcc agaaggcgca
     2101 aacgacacac atttgcaagc attagcagca ctatctcgat tgctgattga tgcagatttc
     2161 gtaggaaaat taaaagaagc aaatacacca gaagaagtcc aagacttgtt ccaaacagct
     2221 gaagagcaaa aagaagcaga agaaaaagca gaacaagaaa cacaagctgc agaagcttct
     2281 gctcatccag aaagaaaatt cgttgttgcc gttacagctt gtccaacagg gatcgctcac
     2341 acttacatgg cagaagatgc tctgaagaaa aaagcgaaag aaatgggtgt ggagatcaaa
     2401 gtcgaaacga acggttcaga aggaatcaaa aaccgattga ctgctgaaga catcgcccgt
     2461 gcagaaggtg taatcgttgc agcagacaaa aaagtagaga tgaatcgctt tgacggaaaa
     2521 gaactgatca atcgtcctgt cagtgatggt atccgtaaac cagaagagct gatcaatcta
     2581 gcccttagtg gtactgcacc agtattccat ggtgatggaa aagaaagcag ttcagaagaa
     2641 ggaaatgcgg atggtactat cggacaaaga atttacaaag atttgatgaa cggcgtatca
     2701 cacatgttac ctttcgttat tggtggagga atcgctattg cgctttcttt catgatcgac
     2761 caatttattg gcgtgccaca tgatcaatta tcaaatctag ggaattacaa tcaagctgct
     2821 agctggttca accaaatcgg acaagctgca tttggtttca tgctgccggt attagcagga
     2881 tttattgctt caagtatcgg tgaccgccct ggtttgatcg ttggatttgc tgctggtgca
     2941 ttagctaata caggcggtgc aggattctta ggtgcactga ttggtggatt cttagctggt
     3001 tatgtcatcg tatttttacg taaattattc aagaacttac caaaatcatt ggatggaatt
     3061 aaaacgatcc tcttttatcc agtgtttggc ttactgatta ctggtttctt gatgttgctg
     3121 gtcaatgtcc ctatgaaagc aatcaatgat gcattgaacg gtttcttgac aagtatgagt
     3181 ggttcgaatg ctgctttact tggtgcatta ttagctggaa tgatggcggc agatttaggc
     3241 ggtccaatca acaaagccgc ttatgtgttt ggtacagcaa ctttagctac gactgttgca
     3301 actggcggaa gtgtcgtgat ggcttctgtt atggctggag gaatggtacc tccattggca
     3361 attttcgttg ctacacgttt gtttaagaat aaattcagca aaacagatca agatgctggt
     3421 ttgacgaaca tcgtgatggg actatcgttt gtcacggaag gatcgattcc atttgcagca
     3481 gctgacccga ttcgtgccat tccaagtttt attattggtt ctgctttaac tggcggatta
     3541 gtcggagctt tcgggatcaa attactggct cctcatggcg ggattttcgt tgtattctta
     3601 ctgagtcacc cggtcatgta tttggtgttt attgctatcg gagcagtcgt atctggtgtg
     3661 atttatggtt tattgagaaa agctcctgca gcagcagaag catagaaata aaaaagaaga
     3721 ggctggtttt tcccagtctc ttcttttttt atagaatcat atagaatcaa gtagtgtaga
     3781 tgaatagttg ttgtaaaata gtttttccat gttcgttaaa atgattgctt caaatttgtg
     3841 atagaagatt tagcctcgat ttcctgcttg attcttggca agtttgcttc atcagtcaag
     3901 ataaccaaaa tatctcctac attcatgatc gtatcaccgt gagtcaagat ttcctgattt
     3961 cctctgcgta tagagattag cagcatattt ttaggccaat tgaagtctcg taccattaat
     4021 ccgtctaaag aactctctgc cgttactgga aaatcaaaag ttgtccttgc accttttagt
     4081 gaggcggtct gagtatttcc aagtagacgt tccaataatg attcgtaaat tggattaccg
     4141 ccgagaaggt cattgacgat ataagcaaca agagcagcca atcctaaagg catcagatga
     4201 ttaagagagc caaccatttc tgtcactaaa ataattgctg tcagcggtgc ttttccaatc
     4261 gctgtgaaat aaccagccat ggcaataacg acaaaatcct tgatgtagat tggatccatc
     4321 ccgaaatcaa ccataatgtt tccataaaag ctaccgatta aagcccccaa tgtcaatatc
     4381 ggaaggaaaa tcccccctgg aagaccacca ccgtaagaaa tcatggaaaa aacgaatcga
     4441 agaacaaaca aaccgataat cataatgatt ggcaaggaag catgtccaaa agctaaaacg
     4501 atttgattgc ctccgcctag agtactaggc cataaataac caattggcaa aatcagcaag
     4561 aaaggaacga acccgtgaaa ataagctggt aaaggcaatc ggttgtaaaa ttttggcaaa
     4621 gccagcaaga ccttctgata aataaaacct aagatgccta gaagtactcc taagatcact
     4681 aaggaaccat aatatttcaa cggcaaagaa ggaaggtcac ctagatataa aacaggtctc
     4741 aagccgaaaa agtaaagaga gacgaagtta gcagtgattg ctgaagcaaa tgatgtcagc
     4801 caaacaagtg gagaaaagga gtgatggatt tcttctaaaa caaagagtaa tcctgcgata
     4861 ggagcattga atgctgctgc aagtccgcta cttgcaccgc tagaaattaa aatcttttct
     4921 tctgaagatg gactccgaaa caattgtcca gctccttgtc caatggatgc gccaagctgt
     4981 atagaaggtc cttctctacc tagaaaaagg cccgcaccaa ctgaaagtac accgccaatg
     5041 aactttttcc ataaaacaga gaaccagttc attgataatt ccccgcgaag ctgtccctct
     5101 acttgtggaa tcccgcttcc cttgatatcc ggttcatttt tgaccagata acccacgaaa
     5161 aatgctgcaa ggatgcagca aatagctagg ccaattaagt aaatcggctg ttcccgactc
     5221 atttcataga tacttcgaac taaatcgctt agcttttcga tagataatcg aaacaggcta
     5281 acgacgattc ctgtcagcag tccaacaaat attcctttta gaataaaata tatctgagtc
     5341 ttatctaatt tcttgatttt tccttttttt tccattcaaa cgcctcgctt tcttttaatt
     5401 tagcgcgaaa tcaacgaatt gacaaggaaa aatccataaa aagaaacaaa tgattttgaa
     5461 ctagctagtt taatgaaagc caagaaaatg ctataattaa caagattgat aaaagaaagg
     5521 atgttgttaa tgtcatccaa agctgaatta ttaaacggga tgaacccccg tcagaaagaa
     5581 gctgttcttc acactgacgg accattactg ctaatggcag gtgcaggaag tggaaagacg
     5641 agagtgctga cacaccgcat tgcctatttg attgaggaaa aagaagtcaa tccttggaac
     5701 atcctagcga ttacttttac caataaggct gcaaaagaaa tgaaagaacg tgtcaatgcg
     5761 attctcgcat ctggaggcga agatgtctgg gtatcgactt ttcactcgat gtgtgtgcgt
     5821 attttaagaa gagatgttga ttttatcggg tataaccgga actttacaat catcgattct
     5881 tctgaacagc tgaccttgat gaaacggata ctaaaagaac tgaacattga tccgaaaaaa
     5941 tatgatcctc gaagtatatt aggaacaatc tctcaagcaa aaaacagcct gcagacccct
     6001 caggattttg caaaaatgca aggcagttat tacgaagaga ttgcggcaaa atgttatgct
     6061 gcttaccaaa aagagcttca atataatcaa tgtatggact ttgacgacct gatcatgaat
     6121 acgattcgat tatttgaaga acatcctgat tcactcacat attatcaaaa caaattccat
     6181 tatattcatg tagatgagta ccaagataca aatcacgcac agtatacgct agtcaattta
     6241 ttggcaggta gatttaggaa cctgtgcgtc gtgggagatg cagatcaaag tatttacggc
     6301 tggcgcggtg cagatatgca gaacatcttg gattttgaaa aagattatcc agatgctgca
     6361 gtaattttgc tagaacaaaa ctatcgctcc acgaaaaaca tccttagcgc agcaaaccaa
     6421 gtcattgaaa acaatagcaa tcgaaaacca aaaaacttgt ggacagaaaa caaagaagga
     6481 aacaagatca cttactaccg tgcagataat gaacgagacg aaacccgttt tatcgtagat
     6541 cggatgcaag aagagattcg aagcaatcat cggaattacg gcgactttgc catcttgtac
     6601 cggacgaacg cacaatctcg tgtaatggaa gaaactctgt tgaaagcaaa tattccttat
     6661 aaaatggttg gagggcacaa gttctacgat cgtaaagaaa tcaaagatat tttagcgtat
     6721 ttgaatgtgc tggcaaatcc tcaagattca atcagttttg agcgtatcgt taattcgccc
     6781 aaacgaggca tcggaccagg atctatcgaa aaactacgca gttttgcttc tcttcatgaa
     6841 tggccactgc ttgaagcagc gcaaaatgtg gatctggcaa atatcggtgg aaaagcagga
     6901 cagcaattag gtgcatttgg agagatgatc caagaagtca ctcagatgat cccatacttg
     6961 acagtgacag aattgacgaa agaggttttg gatcgtagcg gatatctaga agacttaaaa
     7021 atccaaaata cattagaagc acaagcacgc atcgaaaact tggaggaatt tttaacggtc
     7081 acacaagaat tcgacaaaca attcgaacag caaaatgaag aagacgctga tgcaccagaa
     7141 gaaaaactaa cggtcttctt aaatgatctg gcacttgttt ccgatattga caatttagaa
     7201 gaagatgctt cccaagtgac attgatgact ttgcatgcag ccaaaggatt agaattccca
     7261 gttgtattcc tgattggtct agaagagggg gtttttcctt tatctcgagc attgatggaa
     7321 gaaagcgaac tggaagaaga acgccgtctc gcatatgtgg ggatcacgcg agcagaagaa
     7381 gcgttgtatt taacaaatgc tttctccaga acattatatg gacggacgca atataaccga
     7441 ccttctcgtt ttgtagaaga aatcgatcag gagttactgg aaatcgaagg catgcgtcct
     7501 acgcctaaaa aaacacctgt attcgctaaa aaaacagcat atagctataa acagccagag
     7561 acagcggttg ttccctctaa aagtgctact ggaggggaaa ataacagctg gaaacctgga
     7621 gataaagtca aacacaaaaa atggggtcta ggaacagtcg tccgagtaag cgggacgtcg
     7681 aaggatctag aactagatgt ggctttccca agtcaaggag taaaacgtct tttggctgcc
     7741 tttgcgccaa ttgaaaaagc ttagtagtgg agggtaaatg atggaacatg aaatgacgat
     7801 tgacgaagct gctaaacgag cagaagaact tcgtacaaga ctgaaccaat ggtctcgcga
     7861 atactacgta gaagataaac caacagtgga agactatgtt tatgataaag aatacgcaga
     7921 attagtagca attgaggaac aatatcctga tttgatcacg tctgattctc ccactcaacg
     7981 agtaggcgga aaagtattag aaggttttga aaaagtcact catgatattc cgctttacag
     8041 tttgaacgac gttttcagca aagaagagct gatagctttt gatcaacgtg tacaaaaagc
     8101 agtgggacgt gtagttgact attgctgtga actaaaaata gatggacttt ccgtttcttt
     8161 gcgttatgaa gatggaaatt tcgtgagagg tgcgactcgc ggggatggaa ctgtaggaga
     8221 aaacatcact gaaaacctaa aaactgtccg gtcggtccca atcaaattaa aagaaccaat
     8281 gaacatcgaa gtaagaggcg aatgtttcat gccaaaacgg tcatttgtcc aattgaacca
     8341 agacagagaa gcagagggaa aagatatctt tgcgaatcct cggaacgccg cagctggttc
     8401 tttgcgtcag ttagattcta aaatcacagc gaaaagaaat ctggacactt ttttatacac
     8461 agtagctgat ttcggtccta tgcaagcaaa aacccagtat gatgcgctag aagagctaga
     8521 aaaaattgga ttccacacga atcgggaaaa acgtctatgt cattcgattg atgaagtgtg
     8581 gtcatacatt gaagaatatc atgacaaacg tgtggatttg ccctatgaaa tcgatggaat
     8641 cgtcatcaaa gtgaatgaat tctcgctaca agatcaactt ggttttactg tcaaagcacc
     8701 gagatgggca gcagcttaca agtttccgcc agaagaagtt gaaacgctaa tcgaaaacat
     8761 cgaatggaca gtcggtcgaa caggggtagt aacgccaaca gctatcatga caccagtccg
     8821 agtagcagga acgacagtaa gccgtgccag cctgcataac ggtgattata ttaagttgaa
     8881 ggatatccgc ttgaaagata cagttctgat ttataaagcg ggagacatca tcccagaagt
     8941 ttcgcaagtc gtgttagata aacgacctaa agacagcgaa gaataccaat tgcctacaca
     9001 ttgtccagtt tgtggaagtg aacttgtcca tttggatgaa gaagtagcct tacggtgcat
     9061 caatcctaaa tgccctgctc aaatgaaaga aggtttgaac cactttgttt caagaaatgc
     9121 gatgaatatc gatgggttgg ggccgcgcgt actagaacag atgtatgata aaaaactagt
     9181 agcagatgtt gctgatcttt acaaattgac agaagaagag ttattgacgt tggacaaaat
     9241 aaaagaaaaa tcagcaaata atattttgac agcaatcgac aacagcaaag ataattcggt
     9301 cgagcggttg attttcgggt taggaatccg acatgtcgga gcgaaagcag caaaaatttt
     9361 agcagaacat ttcggtgatc tagaaacatt gagtaaaagt gactatgagt cgattattgc
     9421 gcttgatacg atcggtgata tcattgccga cagtgtagtc acttatttca gtaacgaaga
     9481 agtccatgaa ttgatgaatg aattaaaaca agcaggcgtc aatttcgaat acaaagggct
     9541 tcgaaatgcc caattgcaag aagtcgagtc tccttttaaa gaaaaaacag tagtccttac
     9601 tggtaaacta acacgattta ctcgtgaaga agcaaaagag acgatcgaaa atcttggcgg
     9661 gaaagtgact ggtagcgtat cgaaaaaaac ggatatcgtg gtagcaggag aagatgctgg
     9721 cagcaagctg accaaagcac aagaattagg aatcgaagtt tggacagaag atcaaatggc
     9781 agatgcattg gctaaaagtc gctcagtaga agaatgaaaa atgagttttt tcagacaaat
     9841 ttcaataaac aatttcagac acggcactct tgtgttaaaa tagagagcaa agtttttaac
     9901 tcgtttagaa agaaggggta gtatggcgat tagtgaagaa caagtgaaac acgttgccaa
     9961 attagcaaaa cttgcgttcg cagatgaaga acttgcaagt tttaccgacc aattaggtaa
    10021 aatcatcgat atggttgaaa tgctggaaga agtagataca gaaggtgttc catttacgtc
    10081 gaatgtggtt gaaacagtaa acgtgatgcg acaagatcgg gcgcaagctg gatggaaccg
    10141 cgatgaatta ttgaaaaatg tgccggaaac agaagacggc tttatcaaag tacccgcaat
    10201 catcgataat ggggaggctg gcgcataatg acagaattac acaatcgttc tctagaagaa
    10261 ctgcatcata tgcttgtatc gaaagaaatc actgtacaag atttgacaaa agcaacattt
    10321 gaacgaatca aagaaacaga gcctgaggtc gaagcattca ttacattgaa cgaagaaaaa
    10381 gcattggaac aggcaaaagc tttagatgaa aaagggattg atgaaagcaa cgtccttgct
    10441 ggtattccaa ttggtatcaa ggataatatc gtaacaaaag atatcttgac aaccgctgca
    10501 tctaagatcc tttacaattt tgaaccaatt tatgatgcaa cggtcatgga taaaatctat
    10561 caagcagata tgatccctgt tggtaagcta aacatggatg aatttgctat gggtggaagt
    10621 acagaaactt cttatttcaa gaaaacaaaa aatgcttggg acccaacaaa agtacctggt
    10681 ggctcttctg gtggttctgc agcagcagtt gcagctggac aaattccagt ttctttaggt
    10741 agtgatactg ggggaagtat ccgccaacca gcagctttta acggcatcgt agggatgaaa
    10801 cctacttatg gccgtgtctc acgttttggt ttgattgcct ttgcttcatc tcttgaccaa
    10861 atcggaccaa tgacccgtac ggtgaaagat aatgcattag cattgacagc tatcagcggt
    10921 tatgacgaaa aagacggtac ttcttcaggc gtttcagttc ctgattttgc agagggactg
    10981 acaggcgaca tcaaaggaat gaagattgct ttacctaaag aatacttagg cgaaggtgtg
    11041 gcccctggcg taaaagaagc tgtattgaaa gcagcagaaa cattccgttc attaggtgct
    11101 gttgtggaag aagtcagctt gcctcattca aaatacggtg tggctgttta ttatatcatt
    11161 gcctcatcag aagcaagttc caacttgcaa cgattcgatg gtatccgcta cggttatcgc
    11221 tcagaaaatg tgaaatcatt agatgatgta tatgtcaact cacgttcaga aggattcggt
    11281 gaagaagtaa aacgccgtat catgttaggt acattctcct taagtgctgg ttattatgac
    11341 gcttacttca aaaaagcggg acaagtccgt acattgatca aacgagattt tgaaaaagtc
    11401 tttgcagatt atgacttgat catcgggcca tcttcaccaa cagttgcatt cggaatcggt
    11461 gagaacatta acgatccaat cacaatgtac atgagtgaca tcttaacgat ccctgtcaac
    11521 ttggctggac ttccaggtat gtctgtaccg gcaggtttat cagaaggatt accagttgga
    11581 ttgcagatca tcggtaatta ttttgatgaa aaaacgatgt atcaagcggc atatgcgttt
    11641 gaacaagcaa cagaattcca taaacaacaa ccagctattt taggagggaa agacgaatga
    11701 attttgaaac agtcattgga ctagaagtcc acgtcgagtt aaaaaccaac tcaaaaattt
    11761 tctctccagc gccagctcat tttggtgcag aaccaaacag caatacaaac gtgatcgact
    11821 ggggttatcc aggcgtgctg ccagtaatga ataaagctgc gctggaattc gggatgaaag
    11881 cagctttggc attaaactgt gaaatctcta aagatactca ttttgaccgg aaaaattact
    11941 tttacccaga taatccaaaa gcatatcaaa tttcccaatt tgatcaacca atcggtcatg
    12001 acggatggat cgaaatcgaa gtagaaggta aaaagaaaaa aatccggatc gaacgtgtcc
    12061 atttagaaga agatgctgga aaaaacatgc atggtattgg cggttattct tacgttgatt
    12121 tgaaccgaca aggcacgcct ttgatcgaaa tcgtttctga agctgatatg cgttcaccag
    12181 aagaagcata cgcttatcta gaagcattgc gatcaattat ccaatttaca gaagtcagcg
    12241 atgtgaaaat ggaagaaggc tctatgcgct gtgatgccaa tatttctctt cgcccttatg
    12301 gacaagaaga gttcggtacg aaagcagaat tgaaaaactt gaactcttta tctttcgtga
    12361 aaaaaggttt agcttttgaa gaaaaacgtc aagcaaaagt tcttctttca ggaggagaaa
    12421 tccaacaaga aacacgccgt tttgacgaaa caacgaataa aacagttttg atgcgtgtca
    12481 aagaagggtc tagtgattat cgttacttcc cagagcctga tatcccgaaa ttcgtgatcg
    12541 atgacgaatg gattcaaaaa gtaaaagcta gtttgcctga gatgccagct tctcgtcgcg
    12601 aacgctatat ccgtgagttt ggacttccag agtatgacgc aatggtattg acacttacaa
    12661 aagaaatgtc tgattttttt gaagctgttt tggaaaatgg tgcagatgcc aagcaagcat
    12721 ctaactggtt gatgggtgaa gtttctgctt acttgaacag tgagaaactg gaattagctg
    12781 agacaaaatt gacacctgag aacttagcag gaatgattcg tttgatcgcc gatggaacga
    12841 tcagctcaaa aatcgccaaa aaagtcttcc gtgaattgat cacaaatggc ggagaagccc
    12901 aagaagtagt tgaaaaaaat ggttgggtgc aattatctga tccgaacaaa ctactgccaa
    12961 tcattaatga aattttggat aacaaccaac aatctgtcga tgatttcaaa aatggaaaag
    13021 atcgtgcggt tggcttccta gtcggtcaaa tcatgaaagc aactaaagga caagcaaatc
    13081 ctggtgtcgt gaataagcta cttaaagatg aattagcgaa acgatagagg ctgaaaatga
    13141 aaaaagcacg cgtaatttat aaccccacct cgggaaaaga attattgaaa aaaaatttgg
    13201 cggatatcct acaggcatta gaagaagcag gatttgaagc cagcgcctat gcaactacac
    13261 cagaaccaga ttcagccaag aatgaagcat gtagagtcgc agaactaggt tttgatctag
    13321 tagtggcagc tggtggcgat ggcacaatca acgaagtggt taatggaatc gctccgcttg
    13381 aacaccgtcc taaaatggcc atcatcccag ctggtacaac gaacgactat gctcgagcat
    13441 taaaaattcc tagagataat attaaagcag cagcagaagt gataaaaaaa caccaaaccg
    13501 taaaaatgga catcggcctt tctgataaca gttacttcat caatatcgct gcaggtggct
    13561 atttgacaga attgacttat gaagttccat cagaactaaa gagtattttt ggttatcttg
    13621 cttatcttgc gaaaggagca gagatgctcc ctcgagtaaa gccaatcaaa atgcgcttga
    13681 agtatgatga aggggaatat atcggaaatg cttcgatgtt tttcctaggg ctgacgaact
    13741 ctgttggagg attcgaacag atcgcgccag atgccaaatt ggatgatggg aagttttctt
    13801 taatcattgt gaagacagca aatatctttg agatcttgca tattgcagca ctcatgctga
    13861 atggcggtcg acatgtcgat gatccaagaa taatctatac caaaacaagc agtttgttcg
    13921 ttgaaacaga agatgatatc aatcgaccag taatgatcaa cttagatggt gaatacggcg
    13981 gggaagcacc gatgcatttc caaaatcttc atcaacacat tgagttcttt gccaataccg
    14041 atcagattcc tgatgaagca atcacaggta cagatgaaga agaattagag gttgtcagca
    14101 aagaattcgt aaaagaagtc gaacgtttaa cggatgaaga tatcgacggt gacggaaaaa
    14161 tagcagacga aacacctaat caaaagaatt aatcaaaagc cggtgctgaa cgtagtaagg
    14221 tcagtgccgg ttttagttgt gtaattagct gatttatagt agagttcagt aggattgaaa
    14281 aataggctta aaggagcaaa atatgacaga acagcaaata gtaaagaaaa acgaacgata
    14341 tacagtcgaa atcgtcgact tgagttatga aggaatgggg gtagcaaaaa tcgatggtta
    14401 tccattattt atcgaaaatg cactccctgg agaagaagta gaaatactag tagtaaaagc
    14461 aggcaataag tttggatatg gaaaagtcga aacatttatt acacaatcac ctgatcgtca
    14521 gcctgtagaa aacaatgcat tacttcgcat aggtatcgca cctttagccc atttgaagta
    14581 tgaacaacag cttgtcttca aacaaaaaca agtagagcat gtaatggcga agattgctaa
    14641 gatgccagaa gttcctgtac ttccaacggt cggaatggca gaaccattcg gctaccgtaa
    14701 taaagcacag atccctgttc gccgtatcga tggacagtta gccaccggtt tttacaaaaa
    14761 gaatagccat gatctagtcc ctatcgaaga tttttatatc caagatcctg tcatcgatca
    14821 agcaatcaaa gtagtcagag atatcttgca acggttccaa gtacgaggat ataatgaaga
    14881 aaaaaatgaa ggacagcttc gtcacatcat tatccgtcgt ggtcattatt cccatgaaat
    14941 gatggtcgtt ttagttactc gtaaagaaaa attctttaaa tccaaggaaa ttgctgaagc
    15001 tattcatgaa gaattgccag aagtggtttc ggtcatccaa aatatcaatg aagaaaaaac
    15061 aaatgtgatt ttaggagaca aagaaaaagt tttatatggc agagactata tcgaagatca
    15121 gctattagga aaaacctatc gaatctcagc aaagtcattt tatcaagtaa atactcaaca
    15181 agcagagtat ctgtaccaaa cagccatcga gtttgccgag ttagataaaa aagacattgt
    15241 gatcgacgct tattcaggaa taggtacgat tggcttgtct ttagcagaca gagtggataa
    15301 ggtgtatggg atggaagtta ttccagaagc aattgaagat gcacagttca atgctttaaa
    15361 taataaaatc gaaaatgttc attatgaagt cggaaaagca gaaaaagtta tgaaaaaatg
    15421 gcaagaaaaa gggatcaaac catctgtgat cgtagttgac cctccacgaa aaggattaga
    15481 tcatcatttt attgaatcag caatcgaaat gtcaccagaa cgaatcgtct atatctcatg
    15541 taatccggca acttttgcaa gagatgctaa gttatttgca gaagcaggtt atgccgtgaa
    15601 gaaagttcag cctgtagatc ttttcccgca aacccatcat atcgaattag tcgcttcatt
    15661 cgttaaagga gaataaaaat ggaccaaaaa gaaaaaatga aagaattgat caaaaagaaa
    15721 caaggtggag gccgatccaa acaaatggaa acacctaaga atgatcgtaa aaacatgaga
    15781 aaaggcccta aaatctttaa taaatagacg tgctaaagaa agactaagaa gtgttaactt
    15841 ttgttattgt ttcttagtct tttta
//
LOCUS       DAZKNO010000054        15524 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000054 DAZKNO010000000
VERSION     DAZKNO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.054"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..718
                     /locus_tag="ACG8MY_001289"
     CDS             71..718
                     /locus_tag="ACG8MY_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0430498.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            802..1746
                     /locus_tag="ACG8MY_001290"
     CDS             802..1746
                     /locus_tag="ACG8MY_001290"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0430499.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1881..3332
                     /locus_tag="ACG8MY_001291"
     CDS             1881..3332
                     /locus_tag="ACG8MY_001291"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0430500.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3353..4189
                     /locus_tag="ACG8MY_001292"
     CDS             3353..4189
                     /locus_tag="ACG8MY_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0430501.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4189..4848
                     /locus_tag="ACG8MY_001293"
     CDS             4189..4848
                     /locus_tag="ACG8MY_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0430502.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4974..5663
                     /locus_tag="ACG8MY_001294"
     CDS             4974..5663
                     /locus_tag="ACG8MY_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430503.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5669..6625
                     /locus_tag="ACG8MY_001295"
     CDS             5669..6625
                     /locus_tag="ACG8MY_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0430504.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6669..7181
                     /locus_tag="ACG8MY_001296"
     CDS             6669..7181
                     /locus_tag="ACG8MY_001296"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0430505.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7437..7985)
                     /locus_tag="ACG8MY_001297"
     CDS             complement(7437..7985)
                     /locus_tag="ACG8MY_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0430506.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8074..9255)
                     /locus_tag="ACG8MY_001298"
     CDS             complement(8074..9255)
                     /locus_tag="ACG8MY_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0430507.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9491..10999)
                     /locus_tag="ACG8MY_001299"
     CDS             complement(9491..10999)
                     /locus_tag="ACG8MY_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0430508.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11158..11349
                     /locus_tag="ACG8MY_001300"
     CDS             11158..11349
                     /locus_tag="ACG8MY_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430509.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11552..12469
                     /locus_tag="ACG8MY_001301"
     CDS             11552..12469
                     /locus_tag="ACG8MY_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0430510.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12462..13535
                     /locus_tag="ACG8MY_001302"
     CDS             12462..13535
                     /locus_tag="ACG8MY_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430511.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13683..14201
                     /locus_tag="ACG8MY_001303"
     CDS             13683..14201
                     /locus_tag="ACG8MY_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430512.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14182..14574
                     /locus_tag="ACG8MY_001304"
     CDS             14182..14574
                     /locus_tag="ACG8MY_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0430513.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14650..>15524
                     /locus_tag="ACG8MY_001305"
     CDS             14650..>15524
                     /locus_tag="ACG8MY_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0430514.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLP"
BASE COUNT         5130 a         2800 c         3118 g         4476 t
ORIGIN      
        1 catgacagaa aattttttct tcgttatact tgtaatagaa tactatttta taacaaaggg
       61 gatcgaatca atgaaaaaaa ctgttattta tactagttta tttgcagctt tgtttttagc
      121 tgcttgtcaa tcaaatacca atcaaggcac atctgggaat acgtcttcca gtagcgaaca
      181 gcgctcttca caggtatcgg actcctcaac aaaagcaagc acatcttcgt ctagccagac
      241 aaaaagttct agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat
      301 cgaattgttc caagataaat acccagatac tgcaattact agtttggaat tagatagtga
      361 ttggggatct tacttctaca agatagaagg tgtcgatgat caaaaagaat acgaagcaag
      421 aattgatgct caaaatgaaa aaatggagac agagaatcca gaaactctag atcgagatga
      481 acaaaatggt caaaagaaaa aagaagatgg tctggatgtt gataaattga tcacgattga
      541 ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt acggcaacag attgggattt
      601 ggataaagat ttaggtacta cttattggga agtcaaagta aaagacggca atcaaagtac
      661 atcagtcaag atcaatagcc aaacaggcga agtactgtca actgaacaag atgattaaga
      721 atttcttcgg aaaacaagag ttgatttttc ttttagtcat gatacactag acaaaataac
      781 ttgtttgaag gagcgattta cttgagttta gaaacgacct ttgccaatca tatctttgcc
      841 aatcctttga tgaatgcatc tggtgtccac tgcatgacta cccaggaatt agatgaactg
      901 gctcattcag aagcaggagc atttatcact aagagctgta cgatcaatga acgaaaagga
      961 aatcccgaac cacgctattt tgatgttcct ttagggagta tcaattctat gggacttcca
     1021 aacttaggtt tttcttatta tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac
     1081 caaaatcaac cgttattttt ttccattgcc gggatgagtg tccaggaaaa cttggaaatg
     1141 cttgagaaaa tagaaaaaag tgggtttaat gggatcactg aattaaattt atcttgtccc
     1201 aatgtgcctg gaaaaccaca actagcttac gattttgagg ccacttatga aacactgaaa
     1261 gaagtattca gcatcttttc taagccgcta ggaatcaaat tgcctcctta ttttgatttt
     1321 gctcattttg atcagatggc tgatatcttg aatcagtttc cattgaccta tgtcaatgct
     1381 atcaacagtg tgggcaatgg tttgtatatc gatacagaac aagaagcagt agtcatcaaa
     1441 ccaaaagaag ggttcggcgg cattggcgga gagtatatca agccgactgc tttggcgaat
     1501 gttcgcgctt tttatacacg tttgaaacca gaaatccaaa tcattggtac aggtgggatt
     1561 cggacaggac aagatgcatt tgaacatctg ctatgtggcg caagcatgtt acagattggt
     1621 acagagcttc ataaagaagg gccggaaatt ttttctagaa tcataaaaga attaacgcag
     1681 attatgtcag aaaaaggata tacgtcaatt gacgaattta aaggcaaatt acgcaccatt
     1741 tcatagcaaa gagacgaagg tcatgattga tcttcgtctt tttttataaa aaacactcgt
     1801 ctgttcattc ctgtgcaagc gtactcattg tatgttagac gaacttgtgg gaaaataaca
     1861 gaggcaaaaa ggaggataat atgaatgatg caacagaatt agcaaaagag cttaaagaaa
     1921 aagaatttag ttttcaagaa ctagttacgg atatacagaa aaaaataaag aagcaaaatc
     1981 atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag taattcccac gagctattga
     2041 ttccccagtc agaaaaaggg ccattggctg gtatcccatt tcctttaaaa atgctcggtc
     2101 aagaaaaaaa gggatggcta gctacttcag gatctcgttt atttgaaaca caccgagctt
     2161 ctcatacatc taattatgtg cggcaagcag aagcgatagg tctagttcct tttggacaaa
     2221 ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc agtcatctat ggcccagcaa
     2281 gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag tggaggagct gctgctgcag
     2341 tttcatcagg aatcgttcct ttagctggtg ctagtgatgg cggtggttct attagaatac
     2401 ccgcttcgtt ttgcggattg attgggctga aaccttcgcg agggacgatg ccagttggtc
     2461 catatgcttg gagaggatgg caaggagctg ctattgattt cggcttgaca gtttctatga
     2521 gagacacgga ggctttattt tatgggatga gaagtatcca ttccggtgcg ccatatcaag
     2581 tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg gttgaagata gctttttgtg
     2641 ttgattcacc gatccactca cagattagtg aagaggcagt acttgctgta aaaaaagctg
     2701 tagatttcct tggaaaagaa gggcatgatt tgacagagat tccttaccca ctagatggtc
     2761 gtaaactgat cacgtcttat tatcagatga atggcgcaga aacagcagca atgatccgct
     2821 cgattgaaac aggcatccag cgagctgtca gaaaagaaga acttgaaccc atttcttgga
     2881 ctttgttcca atatggtcag aagctctctg ccgctactta catcgattct ctgcatgtat
     2941 gggatgaagc agcaattgtc atggaaaacc tacatcaaga atacgatcta ttcctttcac
     3001 caacgactgc atttaccgcc cctaaaatca gcgaagattt gcaaagtgat gcgataagag
     3061 cacagatgga acacatcagt gattgttctg aagtagaagg tttagacgtt gtttcttcca
     3121 tgtttgagga gagcttgaag atcacacctt atacacagtt ggctaatctg actggacaac
     3181 cagcaatcag cttgcctact catttgacaa aagaaaacat gcctctagga atccaattta
     3241 tggctgccag aggaagagaa gatttgctat ttcaaatcgg ctatctattt gaagcaaatc
     3301 atcagttcca tttaccaaat ccatggaaat agcagaaaca aggagaagaa gaatgaagaa
     3361 aaaaagattg tttgctctaa taactagtgc cttttttatt ttaagcgctt gcggtacagc
     3421 tgccgctaat caggaagata acagctggag taatgtagaa aaaaagaaag aattagtagt
     3481 agcgacttct ggcacactgt ttcccgcatc ttattataat gatcaaaatg aattagttgg
     3541 ttatgatgta gatttggcaa aagaggtggc taaacggtta aatgtcaaaa tctcctttaa
     3601 agaatacaac gtggatggac aaatcacttc tgtttcaaaa agagaatcag attttgctgc
     3661 gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc tccctttcag agactttcaa
     3721 atattcattt gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc actcacttga
     3781 agatcttaaa gggaaaaaag cagctggcga acccaatact agctatatgc gtttagcgga
     3841 aaaacacgga gctaaattag tcacatatga caatgccacg aatgatcagt atttaagcga
     3901 tgtagcaaat ggtcgaacag atgttattct aaatgattat tatctgcaga aaatggccgt
     3961 atctgcttta ccagatatcc ctgtaaaaat actggagaac gtttatttca atgaaaacga
     4021 aagtggtttt ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt
     4081 aaaagagatg aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga
     4141 tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat gtactttcct
     4201 ctaattgatt tttccctcat gtggacttca cttcctttcg tactgcaagg cttggtatac
     4261 acattaggga ttggcttcgt gtcgtttctt ttaggaaatt tggtagggtt gcttttgaca
     4321 gttttaggtc ttttggattg gctgcctctg aatgtattta ttcgttttta tttatcgttc
     4381 tttagaggca ttcccgcact agtcctgctg tttctgttat atttcgggtt accttatcaa
     4441 ttaagtgcct taacggcttc agtcatctgc tttacgatta ccagcagtgc atttattggt
     4501 gaaatctatc gaggatcact agcaggcgtt tcttcaggtc agtgggacgc tggtttagct
     4561 tctggttttt ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc
     4621 tcagtaccgg cactcagtaa cgtagctatg gatctagtga aaggtacttc gttagcagca
     4681 acaatcacag tgccagaaat atttcaaaaa gcaaagatca taggcggtcg gacattcgat
     4741 tatttttctc tctatatttt agttgcactg atttattggg tcatttgtat cgggatcgaa
     4801 aaagtgcaac agtatttaga aaaacgttct atccatcagt caatctgaaa aatagaaatg
     4861 gtgctagcgt ggctggcacc atttctattt tttgtttctt gtatattctg cctagtattt
     4921 taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga
     4981 ccccgctaac catcgaaata gaatcctcgt tataccaata ttgcatcgag caaggcggct
     5041 tggttgtaga agaagtcacg atgcctgatg accaaggaat tgtagatacg cttagctgct
     5101 tgacaaaatt tgatggaagc cgagaatggc gatgctatga gctgaaagtg acaaaagcag
     5161 attttcgctc tacagccaaa ctgtcatttg ttggacatta taattatttt gttttaccaa
     5221 gtaaactata tgaagaagta aaaactgaaa tccctacagg aataggtgtt ttagtgtatc
     5281 gtccgtacac ggttgaagag gagatgcctg cctctggtac atttctgatt gcgaaaaaag
     5341 cagcaaagaa agagcttgct gtaccggaag aagctttgac aaaccgcttc atggcttctt
     5401 tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat tttccaaaag
     5461 atcaattgta caaagaattg aaaagacgtt cagaaaatca agatccattt tctaacgaac
     5521 gatattatga acggttttta gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag
     5581 aagttatcgc gcttcagcat gattatgaat atttgaggca acaacagcag atccgtcggt
     5641 tgcctacaga gccattggag tgaaaaatat gtactatcct tatttaagag gaaaacaatt
     5701 tgacctatta gcattaaaag aagcactgtc gcgaggattg ctctccaata aaatccaacc
     5761 agtgatcgaa ccagtacgtg attccgctac gcttaaaaat gtgatcgaat tatttcaaaa
     5821 aaagcaccat ccaatcgctg tgatccaaaa tccccaagta ggacaattca aattattcga
     5881 tcagcatgtg cacagttgga cagtcaaaga aaatagctct gtcgtccctg cacagatctt
     5941 aacgccagaa aatctcagtg aagtactgga ttcacctcca gcgtttcttg ttttcgatgg
     6001 acagcactac ccaaaagata cagaggtgtg gaaacagttg gcaggagttg attcgaaatt
     6061 tttgatacca gatactagtc gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg
     6121 tgactctttt caaacgagaa agcatgttga aagttatgca gataaaacag atgacttttt
     6181 tagtgatgac tatctttttt tccacgcaga cggatatatt ggattttctg atttcacgat
     6241 cgaaggtagc cgttatttcg ataaagggtt ccctagccga gcgcttgcta tccatctaac
     6301 gtatattgat gcttacggca atatacgcgt gaaacatttt gtctctgaca gtaacgactc
     6361 tgctaaagac caagcattga agtttcttga agcaggagat aaaatgaaaa aatggatcat
     6421 gcgtcaccat catcaactct tgattacttc aggaatgatt gaattattgg ctctttacca
     6481 gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga
     6541 acttattagt cacctgctag atcatccaaa agattggctt aaatattatt caaaagttca
     6601 ggaactatat gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa tagaaaagag
     6661 gaaactggat gtatattcgg caaacaagga aagaagaagt agaagaagtg gtagaaatca
     6721 ttaatgccgc agcggatttc attgcttcgc aaggctctcc tcaatggcaa aacgggcaga
     6781 aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa agaaagctat gtattaatag
     6841 tggaagatca aattgctgga acagctgcat tagtctcagg cgtggatccc gtttacacaa
     6901 aaatagatgg tagttggaaa gaatcaactg gtccttattt atcgattcat cgtgtcgctt
     6961 tagcagaaag tactcgagga aaaaaactag caaaaccgtt cttagaacat ttgttgacga
     7021 ttgcagccag caaaaatatc caagatatcc gtatcgatac ttatccagct aatgaaccga
     7081 tggaaaagac catctattct gctggattca cttatcaagg gatgattgaa tttccttttg
     7141 aacacggcga aagaaaagca tatcaaataa tcatcaattg actataaaat catctgagca
     7201 aagataaaga cattaacact actgaaaaaa acaatcatat gatagccaag aggttgtaac
     7261 agaagtggcc agcttcaaaa aacaagaaag aatttccgaa aatgcttctc aaatttttgt
     7321 ggaattttag cgtattttac gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg
     7381 tgacatagct tttgtcacga tctctcggct tatcctacga gtattattcg atacatttat
     7441 atcaataatc ctttttttat tttatctgca gcttcttgga acgctccatc gtttgacacg
     7501 actaaaagtg aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc
     7561 gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat
     7621 gtttctttag agatcgtaat aaagatagga cggactaccg tcccgaaata ctcatataaa
     7681 tgccaaaaac catttgctgt caaaacggcg taacaatttc ctactgtcag tttcttttta
     7741 atttctgctt tactggaacc atatttatgt tgagcatatt ggtcccactc tgcaaactca
     7801 tttcccttga tcatctgatt gaatgtattg tcagaaacaa aataataatc ctcttgatct
     7861 ttttccccag gtcttggtga tcgattcgtg taagtaatga tcttttgttc cggctgaaaa
     7921 atagcttctg cgagtgaagt ttttccagaa ccagaaggac cgataaatac atagcaataa
     7981 ttcattgtca tttctccttt gatgattgaa caagtgaaaa agctttggaa aatatttcca
     8041 aagcttttaa aagtaacttt gcttttttgt attttatacc agcaagccag tcacaatagc
     8101 acttaaaaag ctgactaaag tcgctgttag caaaattttc aatccgtgtt ttgcgacaag
     8161 atttcctttt tcttcattta atcccttcat cgcaccagaa atgatcccga tagaagagaa
     8221 gttggcaaat gaaacgagga agacagatat aattgctgtt gtacgctcag taaaatggaa
     8281 attaccattt gctaattctg tcatagcaac aaattcattt gtaaccagtt tagtcgccat
     8341 gatactaccg gcatccactg cttctttcca tgggattccg ctgatgaagg caagaggggc
     8401 aaagatgtaa cctaggatct cttggaaagt gatgccgaat atcccgtgga agatagcatt
     8461 gatcatggca atcaaagcaa caaaaccgat aagcatagca gcaaccgtga tcgccacatg
     8521 gaaaccatca agaatgtatt ctcccaacat ttggaaaaat gtctgttctt tgttttcttc
     8581 aatgatcagc tcatcttctt tttcttctaa tacataggga tttacaattg aagccagaat
     8641 ataaccgcca aataagttca aaacaagggc agtgatcaca tatttagaat cgatcagtgc
     8701 catataagat ccgacgatcg acatagaaac tgtagacata gccgatacac tcatggtgaa
     8761 caaacgtttc tcagatagga agggtaattc cttttttatg gaaataaaaa cttccgattg
     8821 ccctaaaata gcagaagcta ctccattata agattccaat ttgcccatcc cgttgatttt
     8881 acttaatcct agaccaacag ctttcataaa taatggaaga atcttgatat agcgtagaat
     8941 accgataata gcagaaataa atacaattgg catcaatacg gaaagaaaga aaggtgcact
     9001 tcctttattt gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag
     9061 atgatcaaac atttttgcca atacagaaac aaccgctgta ccaaaagttg tacgtaataa
     9121 tacgaaacca agaacaagct gtaaaacgaa catcacaatt agtggtttca atttgattgc
     9181 ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg acaaataaac caataatacc
     9241 taataaataa ctcaaataat cactcctatt ccaaattccg ttttatttta cctgttttgc
     9301 aaataaaata ctaatcttta gtgaaatttt cattactttt ttagagtgaa acgcattcat
     9361 tttccattag aaaataaagc gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa
     9421 agtctataaa aaaacgaaac tatcgattaa gctaatagtt tcgttttcaa catttataag
     9481 taaacttttt ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat
     9541 cgattcctag ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg
     9601 cttgtgctcc ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta
     9661 ctgcactgat tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat
     9721 gaagtgtctt tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa
     9781 tgtcatctgc gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat
     9841 ccatcaatga acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca
     9901 cagcagaagg atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag
     9961 ggactaccac gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc
    10021 gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac
    10081 gacggatgat ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc
    10141 ctacacgatt cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt
    10201 tttcaataat acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta
    10261 ctttattgta ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg
    10321 tggctgcttc ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag
    10381 tttcgaactt ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca
    10441 agcaagtagc gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag
    10501 ttgtttcaat atactcattt tttccaaaac catcttctga gacgattttt tctacgttga
    10561 ctggatagtc ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat
    10621 tgataaagac tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta
    10681 ataagccata accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc
    10741 cataatcacc acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt
    10801 cagcttgatc tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa
    10861 gtacacgttt cgcatgcttg tcgtcaacta acttcccacc aaattctaaa tataatttgt
    10921 catagtggtt cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat
    10981 caaaaccaat ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt
    11041 tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac
    11101 caacttgtct agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg
    11161 aaaaaaagac agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt
    11221 tatgaaagaa tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa
    11281 gaaacgcagc taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct
    11341 agtaaataaa aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga
    11401 ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg
    11461 cccgctcttt tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg
    11521 aaaaacagat gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta
    11581 tgagccagac gaactagagg agacgaacgc ggatcaggat acaggacaag aatcgtcttc
    11641 tttttacgat atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca
    11701 ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg
    11761 ccgattgaat gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct
    11821 gatgcgtaag atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa
    11881 catctcgcca acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt
    11941 cggtattgat gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga
    12001 gaaatattcc caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa
    12061 ggtgatcact gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg
    12121 acaaatgact gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga
    12181 aattcaagaa ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc
    12241 ttatattgag acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag
    12301 agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt
    12361 cggtagcaaa gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa
    12421 tgagaatcct aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag
    12481 cagaatggac aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg
    12541 aagaacaaac aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa
    12601 aaatacatga aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg
    12661 cttacgaaat aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata
    12721 tcgtcagttt gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt
    12781 tgatcgaatc agattttcca caattattag aatcatatga tcgttttata ttgaatgaag
    12841 aaccaatctc cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga
    12901 aaccgttgaa tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa
    12961 gacatttttc gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat
    13021 taccgaccat cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag
    13081 agacaatgga aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag
    13141 tggatcgagt attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat
    13201 tagcccatgc aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc
    13261 cacttcgttt gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt
    13321 ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc
    13381 aagtacgatt agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag
    13441 tcttgtcgat ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg
    13501 aattcttggt cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt
    13561 cattcagata attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt
    13621 cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag
    13681 gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta
    13741 ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag
    13801 tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca
    13861 gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc
    13921 tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg
    13981 gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc
    14041 tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga
    14101 tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt
    14161 acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc
    14221 agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag
    14281 gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt
    14341 tcggaaaaag aagtggtctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat
    14401 tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag
    14461 aaaaatcaac tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa
    14521 gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca
    14581 taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag
    14641 gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg
    14701 gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc
    14761 atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc
    14821 ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca
    14881 aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg
    14941 gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt
    15001 gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg
    15061 aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt
    15121 cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc
    15181 ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc
    15241 agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt
    15301 tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct
    15361 tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg
    15421 gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg
    15481 gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctga
//
LOCUS       DAZKNO010000055        15467 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000055 DAZKNO010000000
VERSION     DAZKNO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.056"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(362..1480)
                     /locus_tag="ACG8MY_001306"
     CDS             complement(362..1480)
                     /locus_tag="ACG8MY_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430515.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1713..1835
                     /locus_tag="ACG8MY_001307"
     CDS             1713..1835
                     /locus_tag="ACG8MY_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430516.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1951..3258
                     /locus_tag="ACG8MY_001308"
     CDS             1951..3258
                     /locus_tag="ACG8MY_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0430517.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="ACG8MY_001309"
     CDS             3377..4576
                     /locus_tag="ACG8MY_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0430518.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="ACG8MY_001310"
     CDS             complement(4603..5871)
                     /locus_tag="ACG8MY_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0430519.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5926..5992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6034..6680)
                     /locus_tag="ACG8MY_001311"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="ACG8MY_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7088..7591)
                     /locus_tag="ACG8MY_001312"
     CDS             complement(7088..7591)
                     /locus_tag="ACG8MY_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0430520.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7714..8478)
                     /locus_tag="ACG8MY_001313"
     CDS             complement(7714..8478)
                     /locus_tag="ACG8MY_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0430521.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8585..8860
                     /locus_tag="ACG8MY_001314"
     CDS             8585..8860
                     /locus_tag="ACG8MY_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0430522.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9576..10529
                     /gene="yidC"
                     /locus_tag="ACG8MY_001315"
     CDS             9576..10529
                     /gene="yidC"
                     /locus_tag="ACG8MY_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0430523.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10651..11860
                     /locus_tag="ACG8MY_001316"
                     /pseudo
     CDS             10651..11860
                     /locus_tag="ACG8MY_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(11905..12305)
                     /locus_tag="ACG8MY_001317"
                     /pseudo
     CDS             complement(11905..12305)
                     /locus_tag="ACG8MY_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723846.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(12552..12986)
                     /gene="tnpA"
                     /locus_tag="ACG8MY_001318"
                     /pseudo
     CDS             complement(12552..12986)
                     /gene="tnpA"
                     /locus_tag="ACG8MY_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576373.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13164..13530)
                     /locus_tag="ACG8MY_001319"
                     /pseudo
     CDS             complement(13164..13530)
                     /locus_tag="ACG8MY_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            complement(13544..13829)
                     /locus_tag="ACG8MY_001320"
                     /pseudo
     CDS             complement(13544..13829)
                     /locus_tag="ACG8MY_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13838..14029)
                     /locus_tag="ACG8MY_001321"
                     /pseudo
     CDS             complement(13838..14029)
                     /locus_tag="ACG8MY_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14029..14505)
                     /locus_tag="ACG8MY_001322"
                     /pseudo
     CDS             complement(14029..14505)
                     /locus_tag="ACG8MY_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14973..15326)
                     /locus_tag="ACG8MY_001323"
     CDS             complement(14973..15326)
                     /locus_tag="ACG8MY_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430524.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
BASE COUNT         4556 a         3264 c         2783 g         4864 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6721 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcctggac tcttttaaat
     6781 aagatacgtt ttgcatctta tcatcacccc ctttaatgct tattattcta tgtacatgat
     6841 tcattcgata aataaaagta aaccgtatct tgaacagata gtcaactgca aacagaaaaa
     6901 aggactgtga caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct
     6961 tgcatctgcg gtaataagcc gaaacttcac aaaaatttga gaagtaattt tcgtaaattc
     7021 cttcttattt cttgaagctg accacttctg tcacaacctc aatatggtct attattggtt
     7081 ttacctgtta aatcgaggat tctttcccaa agtaacgttg ccatctctta cgcatacttg
     7141 cagacatggg ctgagccgct tctttgactg cgcagtattt gtccacgaat gtcacaatat
     7201 atccttcttt atacttaggc ggtgcgatcg ttgcacccca catgtgcttc aaaatgatat
     7261 ctcgctcaag atccgacaaa tcggtgattt tttctgcatt ctttacagca attcttggat
     7321 ggatatacgc atgtgtcccc tcgtcaaatt tcgtcgtacg ccaatcatag tagaacagat
     7381 catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca
     7441 gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg
     7501 tgtaattcgc taattttttc actgcttctg tttctaaaag atcttgtaca taagacatat
     7561 attcttcgtc ttcacgccat ttttctgtca taattatcga tttgccacct ttttgaattt
     7621 cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgctct
     7681 agataaaata gctaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacgat
     7741 ccctgctgcg actccgacat tcaatgattc cgcatttcct ttcattggga tatatacgtt
     7801 ctgatcagtc gaagacaaga tttcctgact tactccctga ccttcgtttc ccatgattat
     7861 agcgaaatcc tctgtttttt ctactttgtc taatgcatgt gcttcttcat ttaattctgt
     7921 accgtacacc gtaaaccctt tgcctttcat tacagggatc acttctgcca aatctttttg
     7981 taacacaggc aaatgataat tgcttccctg catgcttcgc agcacctttg tactataaat
     8041 atctgccgtt cctttaccta ataaaacacc agccatacca aacgcatctg ccgtacggat
     8101 catcgttccc acatttccag gatcctgtac attatctagt aaaagccagc ctcctgaaaa
     8161 atctggcgta tcttcagatg ttaacgtcat cactgctaag atcccttgcg gtgtaggtag
     8221 atctgacata gcggtcatga cctcatctga aactagaaaa gtagaaatct ctttgtttac
     8281 agccagccag tctccccatt ctttcatacc tctctgtgtc acaaaaagtt cttttatttc
     8341 tgcatcagac gacgcagctt cttcgatcag atggaaccct tccaaaagat attctgcctt
     8401 ttcttctctg tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttac
     8461 tgattggatc tctttcacat tcctcacctc aactgattat atcatggaat aggtgcttga
     8521 gcttgtttca tcctaaaaaa atcggtatca ttgatttata aaacaggaaa gaaaggtgac
     8581 gaacatgcgc aaagttaaaa tgaatgtcca aggacgtgtc cagggcgtgg gatttcgcta
     8641 tatgaccaag atggttgctg atcagttagg cgtgaccgga acggtaaaaa acgaagacga
     8701 cggctcagtc acaatcgaag caatcggaaa tgacgacatc atccaaaaat ttatcgaaga
     8761 agtcaagaag tctccgagcc caagtggacg agtgcagtat gtggatgtcc aagaagatcc
     8821 gatgattgag gaacgaaaga aatttgatgt ggtgggataa ggttgttttt gaagcgcttt
     8881 gcctcgagta ttaaggagaa acattttcca aatagcttct catattttga aatgtaatga
     8941 cttaataccg agaggcagct gataaaacac cgtcaataag gttgtgaaaa agctggtctg
     9001 catcaagcat taaggactgg ttgtgaaaaa tagctctcca tatttttcaa caggctggac
     9061 ttaatgccgt agatgcaagc ttttgaacat cgtcgataag gttgcgaaaa agctgatctg
     9121 catcaagcat taaggactgg ttgtgaaaaa gtcgcttagt tttgagtatt aaggaaaatt
     9181 ttgtgaaaat ggctttccac atttttgcat aaatttgact taataccaag aaactgactt
     9241 ttgaacatcg tcgataaggt tgtttttgag gcactttgcc tcaagtatta aagacaggta
     9301 gtaaaaaagc tgatctataa cttgcaataa atttgcttaa cccatatttt ccagacagaa
     9361 caaaaaagtt tttagttttt taaataagaa agaaacactt ggaatcactc ttttctttct
     9421 aagtatttct ttcttgattt tttgtctttt ttcaaaattt ttcgctacga aaaagaacga
     9481 atatttatat gaaatttcta tgaagttgat tgaattccat gcgctttttt agtatagtta
     9541 agtttgtgta aaaattatag aagaggaagt attctatgaa taaatggaaa aactggttat
     9601 tgggatccgg acttgtcagt atcctgctct ttttatctgg ctgtgtgcgg atggattcaa
     9661 acggaaaccc tgacacttca ggaatcgttt atcgagtact cgtccatcct atgggccaag
     9721 cgattacata tctagttgag aatttcaatt ggtcttacgg ctgggcagtt attgcaatga
     9781 ctgtgatcgt tcgtatcatc atcttgccac taggtatcag tcaatcgaaa aaaacaatga
     9841 tccaatctga aaaaatgcaa gcgttaaaac cacaagttga agcagcacaa caaaagctaa
     9901 aagctgcaac aacacgtgaa gaacaaatgg cagcacaagc agaaatgcag caagtatatc
     9961 gcgaaaatgg attgagtatg accggcggaa tcggctgtct gcctctattg atccaaatgc
    10021 cgatcttctc tgccttgtat tttacggctc gctacacaga aggtatccgt gaatcatctt
    10081 tttacggcat cgatctaggc tcaccaagta tggtgcttgt ggccatcgca gggggcgctt
    10141 atctgcttca aggatatatt tcaacaatcg gtattcctga agaacagaaa aagacgatgc
    10201 gtacgatgct gatcgtctct cctgctatga tcgtcttcat gtcgatcagt gcgccagccg
    10261 gtgttacact ttactgggta gtcggtggtg tgttcagctg tctgcaaaca ttcatcacaa
    10321 acgttataat gaaaccgcgt ttgaaagcac aagtggcaga agagttgaaa caaaacccgc
    10381 caaaacaagt cgttactcca agaaaagacg tgacagaatc agcagcaaag ccagctgaca
    10441 acaaacaagc aacaaaacaa atcaaacaac caaaaaacag tacaaacaac aaaggccgca
    10501 acgcaggaag gcagcagaag cggaaataaa aatatatgac aaaaaactgt agacaaataa
    10561 aaaaatttct ttgttctaca gtttttttgt tacaattaaa gaaaacgttc gcatttcgag
    10621 gaggaatgta taatgatgtc caaataatca atgtatagtc gaatggaaga agacaaaatt
    10681 tcgctagatg cacttgttcc taaaaatcat ttagtccgta aaattgacaa attattgtca
    10741 tttgatttta tttatctcat tgttgaatca acttactcga ccattggtcg ccctagcgtt
    10801 gatcctgtcg tcttagtaaa attagtattc attcaatatt tatttggaat ttgttcgatg
    10861 cgtcagacca ttaaataagt tgaaacaaac ctagcttatc gttggtttct tgggcttagt
    10921 ttaattgata aagttcctca tttttcaact tttggtaaaa actatgcacg gcgtttcaga
    10981 gaaaccgatt tgtttgaaca gatatttatc attattctcg aataagcagt tgatgctaga
    11041 tttattcatg aagaaatttt gtatatggat tcaactcata ttaaagcgaa tgcgaacaaa
    11101 cgtattttta caagagatat ggcacataaa gaagccattt tataccaaaa acaattagaa
    11161 gaagaaatca atgaggcaag aattgccgaa ggcaaacgtc cttttggttc aatcattcgt
    11221 caggaggtgg tcgaaagaaa atttagcatc gcagatccag aaagtggtta ttatgtaaaa
    11281 acagaacgag aaaaacagtt tgcttattct gctcataccg ttggtgatga aaatggtttt
    11341 gtattagatg tcatgattac gcctggaaat ctacatgata gccgaatgct cgtgccacaa
    11401 atcgaacgcg ttaaatcacg ttatggtgta gctgctaatg ctgcgtacaa aacaccttgg
    11461 aacgtcaagt atttaatcga acgaaaacta cgacccattc tcccttatat cagtttcgca
    11521 caactactcg agatggctat aaaaaatatg tatctaaatc ttttatttgt gaaagttgtc
    11581 cattattgaa aaattgtaca gaaagccaaa aacaccaaaa agaaatacat cgacatattt
    11641 gggaagacta cttagatgaa gcagaacatt tacgccatac ggtgtacaat cgtgaaaaat
    11701 ataaaaaaag aaaagaaacg attgaacgca tttttgcaga cgcaaaggaa aaacatggaa
    11761 tgcgctacac caagtatcga ggcttggaaa aaatcaggtg catacaatgc ttgtttttgt
    11821 cgcaatgaat ctgaaaaaat tagctacttg gcagtgggag catatgtcgt tatatctttt
    11881 attatacaaa aatagacaaa agtatcagtc tacactctta gtaaagagga aactgatgct
    11941 tttgtctaca gtcttagaga gtgggacaat agttttttct tctttacgtc ttttcctctc
    12001 catgtatttt cctaattttc ttaatttaac gccatgagtg ccaagccaat gtcaatcttg
    12061 gctccttgtt tccttcataa gtggaaatcc gtttccgata gatcgaacct tctttttcct
    12121 ctaaaagttt ttttacattc tgctttgaaa taacgccatg tattattcac taaaatttgt
    12181 cggtttcgct cactttttgc tttcgtacat tgactacgaa caggtcagca ccgaccactt
    12241 tcacattcgt aaattttgaa gacacgtata aatccacttt tatcttttct tcgactaaag
    12301 cgttgaccgc aaaagcgaca tctgtttcag aaatatattg ttccatatct aatggtaaaa
    12361 ttttttggtt gatggtataa tttttaaaca taaagcactc gcttcctttc tgattgggtt
    12421 tggtcacttt aatcctacag ggatgagtgc tttttgtgta ccttaaaatc agtaaatatc
    12481 catccgtgaa aacgggtggc tttttaacag ccctagaagg gctcaccact cacagcccta
    12541 tctctcttta actatttaaa tggatctaca tactcttgtt tacttatact gtctctaatt
    12601 cgatcatctg cttcttgttc tcaaatatat atttcaattg ttttttgatt taacccaacc
    12661 gtactcacgt agtatccttt ggtccaaaag cttttattcc catacttata tttcaaattt
    12721 gcatgttttt catggattaa ggttgcactt cgtcctttta agtagcccat aaaactagaa
    12781 atactaatct ttggcggtat tcttactaac atatgaatgt gatcaggcat tgcatgagct
    12841 tcaatgattt caacatcttt cctctcacac aattttctta atatctggcc aatacttact
    12901 cttaactttc cataaatcac ttttcgtcga tacttcgggg taaagactat gtgatactta
    12961 caatcccatc tcgtatgtgc taaactttta tcgctaaagc tatttcgtcc acgcgtagaa
    13021 acgcgcgttt ttttgtttcg tacgctacgc caacgaaact gactaaagtc aataaaaaaa
    13081 gtggggcaat aatcagattt ttttctgact attgtcccac tctcttttta ttactcttca
    13141 tcttctacta ttccactaat taattagttg acagatacaa tgctttcaca tcctgcacga
    13201 acaaactcgg gctctgaacc aatcacttgc gagttgataa attcaatgac cataggaaga
    13261 ttaatccgac ataaagttta atctgttgtc cttccattat cagtttagaa atgatattag
    13321 taggtgtacc gcctaaaaga tcgcatagta ccacaaaatc atttaacgaa tctatgacag
    13381 taagagcttt tgtctgtatg tttcagtgcc atcttctggt aataaaggta acgtgtagat
    13441 attctcttga ggtcccataa tcatttttgt actttttttc aattcctcac aaaattttcc
    13501 gtgactaatc aatatcaatt ttctactcat ttttggctct cccttatttc gaaatcacac
    13561 acaatgcaga taaagccaca cacaagtaat acgataaaaa tcgctcgagt tgggtcatat
    13621 ttttctttcc taacaaccaa taaacacctc gacgatccca gcgagtaaga ttttagataa
    13681 tatcatatcg atatagtttt gcatattcaa tgtcacatcg ccaatcgttg gtaccataaa
    13741 aatttaatat caatcattgt tgcaactaat gctcctacca tgaacactct taataaagtc
    13801 gctacgtccg ttaatacagt taagcgagat tacactagat gatctgttcc tgccaaggca
    13861 agagccagaa gcggttgcct gcccatttgc ctttggtgtg tcggagctgt ccgataaagt
    13921 tcaccgcccg atccgctctg gctttgtcgt agtgacattc cgaaagcatg aatcggcttg
    13981 gtgtgtattc ctttaatttg ggatagccct ttggtctggt ttctgccatc agctaccacc
    14041 tcccaacaga gcctccatcg catcttctgc tccgacacca ccgccatcac cggcaatgat
    14101 acggcttctg gacgaaggcg tcagaccgaa ctgctcggaa aatcggttca taattttcag
    14161 ataggtctgg gcaataaaca cctgtggtac ctgctgccaa tagccggata gggttttcac
    14221 gatgataccg tgctgggtaa tgaattcctc ggcttccttc cagcgggcgt aggcttggca
    14281 ctaaccggca aaggcagcca tatccacatc tgtcaagatt ccgatggcct ccatctgctt
    14341 tgccagacgc ttccattcct tctttgcctc tggttccagc cacttcggac aggtcggtgc
    14401 cttcttttcc ggcgttgact cttttgcatt gagtgggcga ttgcccggat ttccctccag
    14461 cttcttcatg gctgtcgggg ttgattttct tcctctggtt gccacagtcg tcacctccct
    14521 tcaatgtatt tctgccagta gacagagaac agagccaagt cgaaacctga ctctcttctc
    14581 tatctgctaa cttgtatttc agtccactct tctgatgaat cgcacccgca ggattttcaa
    14641 gatgccgcag gcaaattcca agaagtttct gaccgcatca taaccatcgt agccgtgtag
    14701 ccacatcctt ccacctcgcc gccgtcctcg tcgctgatat aaatgattct gtattcgctc
    14761 atctgttgtt ttctccagtc ttttgttttt ttgtagtaca tatatcactc taaaagtgat
    14821 tattatcaag tcgtttgaaa gccataatgt acacaatcat tcgactggtt tttggttcat
    14881 aattgaccgc caaagtgagc aaaagaaagg gacctctgtc tgtttcagaa gtccctttcg
    14941 gtatttggtt cttagtggtt caggctgtat tctcaaacct catactcatc ttcgaggtct
    15001 gcctctgcaa tgatctggcc gatgctgtcg taacgctgga cctgcatctg gatggcatcc
    15061 ttgtctgctc cctcgtccat catcttcagc agcttgtctg attccttttt gtaggcccag
    15121 aaaaggatct cctgaaggct ggtctcctgc tttttcttgc gaagggcgac cttcttctct
    15181 tccaccgcct cgttcaactt ggtgaactct gcccaagcaa ggataccgtc tcgcttgatt
    15241 gcgatctcca catccttcag ctccgggatg gtctttgcac ccttgatctc cttcaaatat
    15301 ttctttgcct tttcgctcat tgccattagc gtttcctcct tgtttttggg attttcccct
    15361 ttggttgtgt gtatattgcc atagtctctc gactatatca agtcaatcca caggcatata
    15421 ctacacaatc atttggagct gtatttgtat acattatgcc gatttat
//
LOCUS       DAZKNO010000056        15199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000056 DAZKNO010000000
VERSION     DAZKNO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.057"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..396)
                     /locus_tag="ACG8MY_001324"
                     /pseudo
     CDS             complement(1..396)
                     /locus_tag="ACG8MY_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            475..651
                     /locus_tag="ACG8MY_001325"
                     /pseudo
     CDS             475..651
                     /locus_tag="ACG8MY_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(827..1396)
                     /locus_tag="ACG8MY_001326"
     CDS             complement(827..1396)
                     /locus_tag="ACG8MY_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0430525.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1470..2759)
                     /locus_tag="ACG8MY_001327"
     CDS             complement(1470..2759)
                     /locus_tag="ACG8MY_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430526.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3086..3961
                     /locus_tag="ACG8MY_001328"
     CDS             3086..3961
                     /locus_tag="ACG8MY_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0430527.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            3975..4298
                     /locus_tag="ACG8MY_001329"
     CDS             3975..4298
                     /locus_tag="ACG8MY_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430528.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4295..5047
                     /locus_tag="ACG8MY_001330"
     CDS             4295..5047
                     /locus_tag="ACG8MY_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0430529.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5037..7208
                     /locus_tag="ACG8MY_001331"
                     /pseudo
     CDS             5037..7208
                     /locus_tag="ACG8MY_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7320..9491)
                     /gene="copB"
                     /locus_tag="ACG8MY_001332"
     CDS             complement(7320..9491)
                     /gene="copB"
                     /locus_tag="ACG8MY_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0430530.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9511..11697)
                     /locus_tag="ACG8MY_001333"
     CDS             complement(9511..11697)
                     /locus_tag="ACG8MY_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0430531.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11697..11906)
                     /gene="copZ"
                     /locus_tag="ACG8MY_001334"
     CDS             complement(11697..11906)
                     /gene="copZ"
                     /locus_tag="ACG8MY_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0430532.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11919..12359)
                     /locus_tag="ACG8MY_001335"
     CDS             complement(11919..12359)
                     /locus_tag="ACG8MY_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0430533.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12433..12972)
                     /locus_tag="ACG8MY_001336"
     CDS             complement(12433..12972)
                     /locus_tag="ACG8MY_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFQ0430534.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13587..15053)
                     /locus_tag="ACG8MY_001337"
     CDS             complement(13587..15053)
                     /locus_tag="ACG8MY_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0430535.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4325 a         3182 c         2696 g         4996 t
ORIGIN      
        1 tttgcgtttt cgatttctcc gaactgatca atgtgggcgt aatttgttcc atctgtcagc
       61 gtagaatttg cagcacctgc tcgagttgga tcaattactg gtttatcctc cgaagtagtt
      121 ggattatcat ccgtaaaacc atgagccaaa tcataggcta gtttttcttt acttacaccc
      181 atttcagaaa gataaccgta aggatctgta tgatcgcccc aaatattttg ttacccataa
      241 atgcgatttg attcctggtt ggttataagg agtgtctaat gttaatggaa taccatattt
      301 cattgctgaa tctcttgcca attcaacgta tactttatag ttcttttcaa aagtttcttt
      361 atcatgtgtg tgttgtaact cgatttgcac aggacttttg gctcacgagg ttgtcctact
      421 ctacatcttg ctgatagata tgctgaaagg aaaacaccaa cattatgttt tgctttttca
      481 gctagaacga gacaagcagg aataccttta gaagatatca aagatttttt aggtcataaa
      541 gatgtttcca ctacacaagt ctatgcccat atttctcccg aagtgaaaaa aagatcaatg
      601 aatcaactag aaaactatat agaagagcaa ataaaaaagc actcaaattg agtgcttttt
      661 taagctcact atcactaaaa ctatcactaa ttttatttat aagcgaacat gtttctatca
      721 ctgatttttt aaatctagcg aaaaaccctt atcttaaagg cgttcgttca attctttggc
      781 aaggtcttca aagcctggct tgcctagaag tgcgaacatg gttttcttat agctcatgta
      841 gaaaaatttt cttgatgatg attttatcca tcaattgaga ttgactgttt tgctctccta
      901 tgatttcagc tatgattgtc cgattttgcg gttttcttct taactggtag ttgattttcc
      961 cagtttccat caactcgcta agcatatcca cgactccgcc aaatccggtc tttttcgtaa
     1021 taaaacctag tgctaccatt cgttcatcta gattttttgc tgtaatcgtc acaccagtaa
     1081 tgagcgagtg ctgtaatgtt tgggccagct cttccatccc ttcagcattg gatacgacgg
     1141 aagctgtttt gttaataggt tcttcgttta tttcttttgg ttctttcctc tccactttca
     1201 ctgtttgacg aaggacatct agttcttgca agagcgtttt gtattgttct tgggtcagca
     1261 tcacgccagc tactttttca cgattaaata tatatacgcc agttcccgcg tctcgcgctt
     1321 gagcaaatac atccatcggc gatcgtttca cttctgtgat cgaagatgtg ggaacttcta
     1381 atttctttaa ttccaatgga tttcctcctt tactaatcgc tttttattat agcacagact
     1441 gacttgtttt gatctttttt ccaaaggttt tatcggtttt tgcctaacca gtaattgata
     1501 tcggtaagaa gtctttcgat accaaatgaa ggatggtaca gatacgtttt ttgatcttcg
     1561 ttatggtcta gtggaaaaaa atgatcggtg atcactagat ctgcataccg aggcggctta
     1621 tttcgagtac atacatgtat agagatccgt tttcgatgtc ttagcgtttt gttcagtacc
     1681 ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa aaaagacgat tttcacttca
     1741 ggagggtgga catctaaata ccctcgttcc tggagtgttt tcagtaaaag catcccccac
     1801 tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc gaaattgata tagtttatct
     1861 ttggaattgg gaaatttttc taatgtttcc tgataataac gaacttcttc tatcagccgg
     1921 tcatggatac caattacgta acgaagccat tcttggtaaa gtacttttat cagctttgat
     1981 ttgctttcgc tctcaaattc gctattcagc caaaacagcg aatcaagctt ttcagtcaca
     2041 atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt tccaccgctt ttcagtaatc
     2101 tggttacagc tcaagcgcaa aattgctaga taattttttt cttttattgg caaaatctgc
     2161 tcctctctaa atagcaattc aggaaacaat tcaaagaata gtgttcgact gtctcctaaa
     2221 atctcgattt ctgccggatc ctttctttgc ttcaataatt ggatgaacat ctcagaaaag
     2281 gactgctctc catcatctgc aaaaaaatgc ggaaaatctt tcaaaaagta aaacagcaga
     2341 tatcttactt ttctttctga accaaagatc tgtgcttttt tataagaaaa agaaaggtgg
     2401 tactcgccaa accactttct caatcgctct tgcgtagaac gatcgtactt ctgctcgtaa
     2461 ggttcattta atagcagatt tttcagtaaa caaaacgggg gtgattggat cagaaaaaca
     2521 aacaagagat aaggttcgct atatccttga tcataaacta actgaacggt tttcttatga
     2581 agaatcagct gaatgggaca gtcgctttcc gccaaacgtt tcagatgccg atccactgtt
     2641 ttaacatcga ttttcagttc ttcagctaaa tcagcttttc gccagatcaa atctttcatt
     2701 agattgatta gtgaaaataa attctgtgca ttttttgata atagtttttg ccatctcatc
     2761 gatcatttct cacctgattt ctaaacatat tgtttgatca aaatgagttc actcctaaaa
     2821 agataaaaaa ctccctttct atttatttaa atacgcaaat cttcctcatt attttttatt
     2881 ttactcaatt taatcgtatt ctcatttcaa aacgaatgta ctttaaaaaa acataataag
     2941 atcactttgc ttgtaggatg tattcccttc cattacaatg aaataaatag cgattttatg
     3001 agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc aaacaaaaaa tcgatcagtt
     3061 gatataagaa ttagaaaaag cggtcatcgg caaacgttca actatccaat tgatgatcgt
     3121 ggccctatta gcaggtggtc atgtcttatt cgaagatatt cccggtgtag gtaaaacgtt
     3181 actggtcaaa gcactagcaa aagctgtaca gggaaccttc agcagagtcc aatgtactcc
     3241 tgatctgctg cctagtgata ttcttggttt ttccgtatac aattcacaat caaaagagtt
     3301 tgaatttcgt ccgggaccga tctttaccac gatcttgttg gcagatgaga tcaatcgtac
     3361 gactcctcgt actcagtcag cacttttaga agcaatggcc gagaatcatg caacgatcga
     3421 caacaataca taccctctag acaatcattt ttttgtctta gctacgcaaa atccaatcga
     3481 gtacgaagga acatatcctt tgccggaagc acagctcgat cgttttttat tccgactaca
     3541 aatcggttat ccttcttttg atgacgaact tttgctactt ttggataaat tcgaaaaagt
     3601 gttggataac ttaaatgaag tattgaacag ttatgaaata gaagaaataa aaacaaatgt
     3661 ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc ttgcagttag tgtcagcaac
     3721 aagaagtcat gctgccatcc agcttggcat cagtccacga ggaagccttt cctttattca
     3781 ggcagcaaaa gcttatgctt taatccatgg gagaaattac gtcacgccca aagatttgca
     3841 ggatttagtt ccttacgtgt tttcccatcg cctgattttt tacgatcgtt cattgaagga
     3901 agacgaaaag aaacaaatca ttcatactct tgttgcacaa attcctattc ctgtgaggta
     3961 gattgaggta gacaatggaa aaaatcaaag attatttcat taattttgtg ttatttctgg
     4021 tatatttcat cattgtgctt tttgctcttg tattcaacaa ttctttaggc tggttcttgt
     4081 tggccttttt tactctttat gtatcatgga gcttgatttc attaagcagc tttctaaaga
     4141 aactttcggt tacttgtaca ttgcagccac tcgtttatcg caaaaaaagt atgcaggtta
     4201 ttttttccat agcaaaaaag agaacttttt ctttacctct ccctcgttta caaatcggct
     4261 tcccagcaat ctttgcagaa aaaaacaaga gattttgatt cttacaaata aaccgcaaga
     4321 agtccaattg ctttggcagc caaaagaaag aggatgccat gaaacattaa ctgtcatttt
     4381 tacagcctat gatagtctag gacttttcag aaaaagtgca aaaagagaga tctcttttcc
     4441 tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa aaattacaga gagttctgga
     4501 aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat tttcgggaac atcgcgctta
     4561 tcgtccaggt gattctttca accgaatcga ttggaaattg tctgctcata ctcaagagtg
     4621 gttataccgt gaatacgatt atcaagaaga agaatgttct cctctttttt gcttttgggg
     4681 ttcgcccagt ccaaaatttg aacctctgct agacttgtat ttttctttat ggcatttgag
     4741 aaaagaaaag cagccggaac ttctgatatt aggcaaccgc gcctttcatg gcaaagaccc
     4801 cggtcatacc tattttgctt ctttggaagc tggcgatgag aaagcttttt tcgcacactt
     4861 gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct gagaattctc ctgaagtctc
     4921 agaagctatt gaaacattgt caaaagatcg aacggcttat cttgtttatt tcgtagaaaa
     4981 acaactcatg gtccaagaaa aagacaagat atgttcattg ccaggaggtg aatggattga
     5041 cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt tgtgatacta agtgtaggtg
     5101 ctccgatatt tgtagtagct aatcaattgc aaaacagcta ccttcttatc ctaactattg
     5161 gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg ggtacgtttg ctgctttctt
     5221 tgccagtaat gataggttgt ttgtttttat attttccttc gaagacattt tccatattat
     5281 ggatcttaca gtttttttcg tcagttacag aggaattctt ccatctatcc caaggtaaac
     5341 tttattattt gccagaaaag acagcttttt ttctcataat gttgctgatc tatttattta
     5401 ttacattgac cgtggattac gactattggt acgcaccctt tctttctttg atggcttact
     5461 tcatgatgct taccgttttt cggaaaaaag atttactgta cgaaatgatt gcggtagtaa
     5521 ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc catccggttg ttgtcgggaa
     5581 acactcgatt ccagtcattg actacagttc tgcttatttg tttcttaact tttagcgcta
     5641 tcttgccttt atatcttccg aaagtacacc aatctctcga ggaaacgagt gaaccgatcc
     5701 gagaatatct taatgatgaa gggctttatg acacactcca tgagtatcaa ttaaccggaa
     5761 gtgcaagaac aggcttcagt gagaatgatg aacagcttgg tggaccttta tatgataatg
     5821 gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta ttggaagatc gaaaataaaa
     5881 atcattacac cggttctgga tggatagaag atcaggacgc acccatccaa ccaatccaag
     5941 aattccctgt tgacatcacg agctaccaaa ccgttataga agacaaagaa aacattacat
     6001 tgaccctaaa aaaacaagct ttcttacctt atccttatgg aaatactacc ttgactgatt
     6061 tttcattagc tagtggacaa acgctttact atttgcctga aagccagcga ttcattcctg
     6121 tcccgaatga atcgacacgt tctgcaactt tacaaacaga aaggttagtt tttacgagag
     6181 aagaattaaa aagcagtcag ttaaaagctg atgatttaga aaatcagcag acaaatcttc
     6241 aattgccaaa aagtttgcct aaacgtattg aggacttggc ggtcgaattg accaaaaatg
     6301 aagcaacttt aattcgtaaa gtcgaagctg tctaacagta tttaaaaaca agcggaaatt
     6361 tccgctattc caaaacggat accgggcata ctccgaaaga acacgattat gtggattatt
     6421 tcctctttga ttcaaaagtc ggctattgtg acaacttttc aacttctatg gtcgtccttc
     6481 ttcgaacact tggtatccca gctcgctgga caaaaggttt tacaaatggg ctcagaacag
     6541 gcacagataa tcaagggaat gaggaataca gcattctcaa tagtcatgct cattcttggc
     6601 cagaggtata ctttcctgga tttggctggc ttccatttga accaacccct tcatttgcca
     6661 atccaatggg ttcacaacca gttgctgaaa caaccgaaac cgtccagcct gctatcgaac
     6721 aaccaaatga aagcgaaaat atggcagaaa ctatttccag tgagagatct gaggtcattc
     6781 catcagaaga aacaacactc acaccagctt ataaaatcgt cttattcagc cttggagtgt
     6841 gtttatccgt cttgttgttc tttctattgt tattcagaaa atctctgcgc tggacaagtt
     6901 atcttatcta tcttcatttt tttgcttcta ctaatacgat tgcatatcaa aaacttctta
     6961 accctttgga aacattggta ccgcgcaaac ccgcagaatc tttgcaggaa tatagcctgc
     7021 gaatagaaca agaattcaag ctagatctgc gtttcattcg attaactact caatatgaag
     7081 cagaaatata tggacgacaa gcagatccgg taaaattcga taaaaaaacc tatttagaaa
     7141 taacgcgtat aataagaaaa acggtataca aaaaaattct gacaaaaggg agccgcttca
     7201 aaaaataaga taaaatttct ggagaatcta cttctggaat gccgagaacc agctgattga
     7261 gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg tcacaacctt ttttcattat
     7321 tatttgattt tcaatgtcaa agcatttaga gctacaacta ccgtacttaa cgacatcaag
     7381 acagcaccaa ctgctggact taatatgata ccaattggcg caagaattcc ggctgctagc
     7441 ggaattgcaa gaatattgta acctgccccc caccagaggt tctggatcat tttctttcgc
     7501 gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg gatcgctgtt tgtcaatacg
     7561 acatccgcag aatcgattgc gatatctgtc cctgcaccga ttgccatccc gattgaagca
     7621 cgggctaaac ttggcgcatc attgatccca tcacccacca tgattacttt tttcccttgg
     7681 tcaaggtatt tctggacaac agcttcttta tcatctggta ataaaccgcc ataatattct
     7741 tcgatcccta aataatctgc tactgcttgg gcagcgtttt tattgtcacc agtaagcatg
     7801 acaggagtga tttttctttc tttcaatgtt cggataaatt cttttgcttc tgtttttact
     7861 acgtcaccta aagcaacgat tgcagctaga tgattttcta aaatcaggaa gctgatggta
     7921 tttccttgtt cttggtaagg tttcaataat gcttgttcta cgtttaatcc gagacgctca
     7981 acttcttttt cattaactat tttcacttgt tgttttttga ctgttgcctc tagcccgact
     8041 cctgaaagat tttttagatt ttcagcttga taaggcttga tttcatgttc tttcaaataa
     8101 ttcataatgc caacagccaa tggatgattg gcattcgctt ccaaggctcc tagatattga
     8161 aggatttcat ttttattgaa cgcatcatca agtacttcga cacccgttac agtaaatttt
     8221 ccttctgtta gtgttcctgt tttgtccagc atgatcacat ctagatcatt ggcttgctcc
     8281 agtgcattgc ggtttttcaa aagtaatccg tttttagctg ctattgatgt ggagcgagca
     8341 acaactaatg ggatagctaa acctagagca tgaggacagg cgatgataaa gactgttacc
     8401 atacgttcta atgcattagg aagatctgtc aagaacaacc aggcgatgaa tgccaggata
     8461 cccacaatta atgcgacata aaacagccat tttgccactt tgtcagataa tgactctagc
     8521 ttggattttt ctccttgtgc ttttcgcacc atttccataa ccttcgctag ataaccattt
     8581 tcaccagttc cagttacttc gatttcgatc gttccatccc cattgataga accgccaatc
     8641 actttgtcat tggcttgttt tttgattcct cttgattcgc cagtcacagc tgattcgtca
     8701 acaattgttt cacctttcaa gattacacca tctgttggca ttttatcacc agaccggacg
     8761 atcaaacgat caccttcgtt tacttctttc aatgaaactg tctcttcttt tccatctttc
     8821 gtcaagcgtt tcactgattc tggcagtaat tctgctagtt tttgtaaagc attgctggca
     8881 ttcgacacag cattcatttc aatccagtga cctagtagca taataacgat caaagtagcc
     8941 aattcccaga agaaatccat gacgtgtgta tgtggattga gcagatttgc gataaacgaa
     9001 tagacactat aaatatatgc aactgtaatc cccatcgcga tcagtgtcat catagcagga
     9061 cttttttgtt tcaattccat cttagctcca cttaaaaatg gctgaccgcc atagataaat
     9121 agtatagttg ctaagatcaa tacgagccaa tcagaaccag gaaacgttac ttgaaatgga
     9181 aattccattc ccatcattgg tgaaaaaaga atgattggga tagctaggat aagcgatagc
     9241 caaaattttt gtttgaaatt ccccatgtgc atggaatgat ccatgccgcc gtgttcgtga
     9301 tccatgtgca tcgaatggtc catttgtttg ctgtttgcta tatctccatg gttatgtccc
     9361 atgtgcatgg aatgatccat ctgactatgg tcatgtttca tctccatctg tttttcatga
     9421 gattctgtac tttctttgtt tactacttct tttttatgct catgtttttt ctccatatcg
     9481 ttcccattca tttttactcc atcccttctc ttactttttc atgtgtcgat ttaagctcag
     9541 ggagttcaac aatacactga cggaactaaa cgccatcgct cctcctgcaa tgattggatt
     9601 caaaaagcct agtgcggcaa aaggaatacc aatcgtatta tagacaaaag cccagaaaag
     9661 attttgtttg atttttctca atgtcaacct agacaactcg attgtttgtg caatggatgc
     9721 caaactgcta ttcatcagcg tcacatcggc tgtttccatc gcaatgtccg ttccacttcc
     9781 cattgccata ccgacatcag ctaatgctaa ggccggtgca tcattgattc catcgcctgc
     9841 cataccgact tttttgccat ctttctgaag tttttctacg taggctgctt tatcctctgg
     9901 aagtacttca gcaaagatat gctttggatc aataccaact tgtttaccaa tcgtttcggc
     9961 tgccagtttg ttgtctcctg tcagcataaa aacatctaat cctttattct ggagctgctt
    10021 gattgcttgt ttggcttcta gtttgatttg atcagccacg gcaatccacc cgatcacttg
    10081 cttttcatca gctaaaaaca tgactgtttt tccctgctgt tctaattcta acgcgtgttc
    10141 ttggtattca tcaaatgata ggttcaattc aattaatcgt ttacgtgtac ccgcaaaata
    10201 gcgtactcca ttaatcgtac cgctgatccc agcacctggg tgggctgtaa aatcaatcat
    10261 atcgtatgtt gctgcttctt gcagttttcc gtattccacg attgctttac ctagtggatg
    10321 ttcagaagct tgttctaaag tataaaaaat agataggact tcttttgaac cgactagatc
    10381 tgtaactttg ggctttcctt ctgtaatcgt tccagtttta tccaaaacaa tgctgtctaa
    10441 gtgagcagct gcttctaatg cttctccgcc tttaattaaa attccatttc gtgcaccaac
    10501 acccgttcct accataatag ctgttggggt cgctaaacct aatgcacatg gacacgcaat
    10561 gactaataca gagacactat ggatcaatgc gagttgccaa tcaccagtga tcaaacctgt
    10621 agctatgagc gtgataaacg cgatgactaa tacgatcgga acaaaaatcc ctgagatccg
    10681 atctgcgatt ttttggattg gggctttgct cccttgagca tcttccacca tttgaatgat
    10741 ttgcgctaat actgtctgct tgccgacttg tgtgacttcc atgtgtagca gaccattcgt
    10801 attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt tctacaggaa gattttcacc
    10861 agtaagcata ctttcatcta tcgcactgct tcctgaaata attcgaccat ctgctggaat
    10921 ctgctcacca ggacggatga ctagttgatc accaacgact acttcttcaa tcgctaacgt
    10981 ttcttctttc ccgtttctta tcacttgggc ggtttttgtt tgaagagaca tcaattgttt
    11041 gattgcatta cctgttttcg tttttgctgt atgttccaaa tatttgccga gcaaaatcag
    11101 cgtgatgatc atactgctgc tttcaaaata aagatcttgg gggtgtccac ggaaaaatcc
    11161 gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt gctaccaata catccatatt
    11221 tggtgctttc gtcttgattg catggtacgc acctttataa aaacgagcac cgacataaaa
    11281 ttggacagga gcagacagca ctaactggac gattggaaaa tgaaaaaagt gaacaatcgc
    11341 cgcatggctt cctaacatca tagcaatcat tgacaacatc aacggcaaag tcaaaaccgt
    11401 actcaatata agatcgaata gcattttttt caaatacgct tgcttttctt ctgcaatttt
    11461 ttgtttgtgg gcttcatcgt acaaaattgc accataaccg atgttttcta cgctttggat
    11521 taatttttca gtggtcgtcc cttcatattt gacacttgct ttttcagtgg ccaagttgac
    11581 tgtggcattg accacaccag gttgtccgtt cagctctttt tcgatcctag cagaacaatt
    11641 tgcacaagtc atacctgtaa tgacgaacgt atccattttg gtttctgctg ttgccatcaa
    11701 atcacctctg ctttataacc caatccattg attgcttgac aaatcttttc ggattgaact
    11761 tttgtttcat caaacttgac cgtaccgttt gcttttttca aatttacttt cactttttgc
    11821 acgccgtcaa gtgcgcttac tgtttcttct actcttgctg cacaatgatt gcagctcata
    11881 ccttcgattg aaaattgttg tttcattttt tcttcctcct attgatgttg tttacattca
    11941 cattgacctg ggatacagtt acattcgatc gtatctactg gttgcttttg ttccagttgt
    12001 ttcaaaatct cattgatatc ttcttgagtc aaagtggctt cttctactag atccgcaatc
    12061 gtctctccaa tcctttttgc acagatatgg gaaaaaagat tttccgtcgc actgcgaact
    12121 gtttccatct ccgagacagc tggatgataa atgaattttt ttccttcttg ttctgtccag
    12181 atcacttctt tttttaccaa acgtcccagc aaagttttga tcgttgctgg tttccaatcc
    12241 atcgaatcag ctaagatctg cgtgatttct tgggctgtcg tctgtccaag tgtccaaata
    12301 acacgcatga tctcccactc tgaatcactg atctttactg gcgttttgac tttttccatt
    12361 tatttcacac ctttcgttta caaatgtaat cattagctta tgaatacaga ttacgcttgt
    12421 aaacaaaaac tgtcaattat ttttgctctc tattttcaat ttagttaaaa attcttgttt
    12481 ctctcgtctt gttttaagga tgatcagttt atttcttgca ttagctactt ggatcagctg
    12541 ctgtattttc ggttcattgt tttcttcaaa atgccagata aaagataaaa attctaagta
    12601 ttccctgttt agtttctctg aaaatgattc aggcatatct ggccgagatt ttttgtttat
    12661 ctttcttttt agagaacgcc aaactactcg gtagatggcg agataacgcg gcactttaag
    12721 ccaaatgatc ttatctgctt ctttaatcct ttcctctaat gtagaagtat aatttccttc
    12781 aacaatccat gacggattag ataccataaa cgattgctgt tcttgcaaaa accacttttt
    12841 tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct agcggcagta gcggaaagtc
    12901 ggtgatttct ttgatcttcc ttgcgagggt cgattttccc gacccacttt gtccaatcac
    12961 gatgatcttc attcttctcc ctcacttctc tttggaattt ggttcgatca taccacttat
    13021 aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat
    13081 aaatggtgtt caatcagctg accttcagca ttaactcaaa aaacggcaaa atatgaggag
    13141 ctattttttc gtttgttcct ctaatactca ggtcaaatcg ctgattttac aacctcttcc
    13201 ttaaacggtg ttctcctgcc aacacctcgg tcacaaggca caatagtcaa caaacatgag
    13261 aagttgtttt ttgaaattgc tccttgtgac tcggagttaa acggcaggct cactacctct
    13321 ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccagcaaa acgaaaaata
    13381 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
    13441 ctctgattca cggtgttcca gaagtagctt ctgcgaaaaa caagccgaaa cttcacaaaa
    13501 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
    13561 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
    13621 tctttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagaca
    13681 attggtgtga tgatattcgt gaataattgg aaccaccatg ctttaggatc agcagtataa
    13741 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
    13801 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
    13861 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
    13921 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaagatc
    13981 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
    14041 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
    14101 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
    14161 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
    14221 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcgct agcaaacagc
    14281 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
    14341 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
    14401 tccgctcctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
    14461 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
    14521 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
    14581 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
    14641 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
    14701 tttccatttc cttggactgc ccaaccgcca gcaatcagga ttgcttgagg cttttgtgcc
    14761 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
    14821 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgaccct
    14881 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
    14941 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
    15001 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
    15061 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
    15121 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
    15181 ttttccatca aaaaaataa
//
LOCUS       DAZKNO010000057        15179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000057 DAZKNO010000000
VERSION     DAZKNO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.058"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8MY_001338"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ACG8MY_001338"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0430536.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8MY_001339"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ACG8MY_001339"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0430537.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8MY_001340"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ACG8MY_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0430538.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8MY_001341"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ACG8MY_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0430539.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8MY_001342"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ACG8MY_001342"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0430540.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8MY_001343"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ACG8MY_001343"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0430541.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ACG8MY_001344"
     CDS             complement(8053..8523)
                     /locus_tag="ACG8MY_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0430542.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ACG8MY_001345"
     CDS             complement(8569..8994)
                     /locus_tag="ACG8MY_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0430543.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ACG8MY_001346"
     CDS             complement(9061..9330)
                     /locus_tag="ACG8MY_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0430544.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ACG8MY_001347"
     CDS             complement(9341..10936)
                     /locus_tag="ACG8MY_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0430545.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8MY_001348"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ACG8MY_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0430546.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8MY_001349"
     CDS             complement(14489..15049)
                     /gene="pth"
                     /locus_tag="ACG8MY_001349"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0430547.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3921 a         3436 c         2693 g         5129 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggc
//
LOCUS       DAZKNO010000058        14926 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000058 DAZKNO010000000
VERSION     DAZKNO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.059"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(672..3821)
                     /locus_tag="ACG8MY_001350"
     CDS             complement(672..3821)
                     /locus_tag="ACG8MY_001350"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFQ0430548.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3834..5033)
                     /locus_tag="ACG8MY_001351"
     CDS             complement(3834..5033)
                     /locus_tag="ACG8MY_001351"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFQ0430549.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5023..6618)
                     /locus_tag="ACG8MY_001352"
     CDS             complement(5023..6618)
                     /locus_tag="ACG8MY_001352"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFQ0430550.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(7127..7909)
                     /locus_tag="ACG8MY_001353"
                     /pseudo
     CDS             complement(7127..7909)
                     /locus_tag="ACG8MY_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7974..8810)
                     /locus_tag="ACG8MY_001354"
     CDS             complement(7974..8810)
                     /locus_tag="ACG8MY_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0430551.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8828..10237)
                     /locus_tag="ACG8MY_001355"
     CDS             complement(8828..10237)
                     /locus_tag="ACG8MY_001355"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0430552.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10251..12074)
                     /locus_tag="ACG8MY_001356"
     CDS             complement(10251..12074)
                     /locus_tag="ACG8MY_001356"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0430553.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12917..13243)
                     /locus_tag="ACG8MY_001357"
     CDS             complement(12917..13243)
                     /locus_tag="ACG8MY_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430554.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(13289..13651)
                     /locus_tag="ACG8MY_001358"
     CDS             complement(13289..13651)
                     /locus_tag="ACG8MY_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0430555.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(13688..13903)
                     /locus_tag="ACG8MY_001359"
     CDS             complement(13688..13903)
                     /locus_tag="ACG8MY_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0430556.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            14030..14365
                     /locus_tag="ACG8MY_001360"
     CDS             14030..14365
                     /locus_tag="ACG8MY_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0430557.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14441..>14926)
                     /locus_tag="ACG8MY_001361"
     CDS             complement(14441..>14926)
                     /locus_tag="ACG8MY_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430558.1"
                     /translation="SYEWVKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGL
                     SWQGTYLMTEKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLY
                     NVKASSSFLIYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVL
                     N"
BASE COUNT         4420 a         3122 c         2354 g         5030 t
ORIGIN      
        1 aatcattttt tattggatta ttcccttgtt cgatatagac ccaaattgat ctatcaataa
       61 ttatcaaact gagtcatata gagacaaaaa tttcaaaaat gacctgttaa atggtcatcg
      121 aatatcattt aaaatcaata cgacggagtc agacacggag tcaaaacaag cccgctgaga
      181 gtaaaaaata tacagtccca taggtaaagt gccacaggac tgtatatttt tttatttgag
      241 attacctgaa atgattccat ttaattttta tctcattttt tcctactagt tagtatcaaa
      301 tactctccta ttttctcaat aaactatttt gggttacatg gtatctgaaa cagctaaaac
      361 cttgtcatta tagcaaaaaa tcactttatc tccgtttcat ttcagataca tttcacttaa
      421 gcggattcca aaaaaacaaa agaaaaagga acccctagtt agcctatgct ttttattggt
      481 tcctctcaaa acacggtcat ataacaacgg ggtggggtaa atgaatgaaa aaggtggagg
      541 aaacattatt caatagctgt cttaaatact tcgttataaa atgtgaacaa ataaaagaca
      601 tctatcccta ttttctacac ttccacttga ctaccatctt tgaatcgtac aattttctta
      661 actcttctcg tttacctcaa ttcattctca agtggaatca actcatcatc taacatttta
      721 cgccatgctc gcttcatttc ttgtgggtat ttaaatggct tcgcttctgg atgtttggct
      781 ttatatcctt cgtaatcctt actgttgata attgctttca tattaggaat tggatcttca
      841 cctggtagat attgcagatt agaagcatgt aattctttct cagaaacaaa ccactcattg
      901 gaaaagacat gaatcaaatc atcactcgct tgtgtaaaat agcgacgttt aatgacaaag
      961 atatcttcat tactatctaa agtccccaca tcaaatgcat ttaaaatttg tcggtaaact
     1021 tctttgactc cttctgtttt attcaattta gccaaagctt tttcaatttc ttctcgtgca
     1081 tctgaactat tcgcagcata agttcctagt aattgatcaa tataagttga atcaatatca
     1141 tacaacttag tggtattttc actataaaat tcgatcgttg aaaaatcttc tccttctttt
     1201 tctggttctt cctctattaa tgaacccttc acggtctcgt acacgccaat ttgttcttcc
     1261 aaaagttgaa tttgattgtt caaagttgga gatttcacca tgtcatcatt gtaatcgtca
     1321 taagtcacta acgattcata agcacgattc aattcttgaa atgctttgac gtactcgata
     1381 cggatttcaa gtggcgtatg ttcatcaatg gattccatgg atgaaacctc ttttaactta
     1441 ccatatgctt gtttgaatct ttttttggat tcctgataac ttggataaat caaacctgat
     1501 tcttcttttg cttttgaata cagttttgtc gcatcctcga cattcttctc cattgtcgct
     1561 ggcttacgaa acgtcacaat caatcctttt gtcttatcag gatacgtacg attcgtacgt
     1621 gaaaaagctt ggatcaatcc tgcatactct agattacgat ccacaaatag ggtttgaatc
     1681 gtaggggcgt caaaacctgt taatagacga tccactacaa tcgctaaatc gatttgtttc
     1741 ccaaattgtt tgaattccgc tctttttcgt gccaaacgat tattgatgtc tccgttataa
     1801 cgctcaatgt ctgctaatga ccaggaagta tcataatact gattatattc ctcaataatt
     1861 ttttgcattt cttcttgttg ggcacttgaa tctcggctat tttcttctaa tgaataggta
     1921 atagcgattc gtggaaaatc aggatcctcc atcgttcgtc cttcacgaat gagctgattg
     1981 ggaaactctt ttgttaacca atcaggctct ttcgtcatct ctttgatggc ccgataatac
     2041 tttttggcca tgtcaatgga actcgtggtt aagattgcag atttcgttgg cctgccattt
     2101 cgaaaatcaa attttgtata ggcattatct ggacggaaca ttttatgaat gactttttgg
     2161 atatgttcat ctgcttcaaa aacagctggg tccaaataac tttctttttt gacaggttcc
     2221 atttgatcaa tcatacgatt gatctgttct gatgaatact ctgcatatgt ttctacttct
     2281 tttaatttcc gatagatttt attctctagt gaagtcggct ccacggtgtt ttcatgctct
     2341 acttgaaatc ctaagacaga gccatcttct agcgcattct taatggtata ggtatgcaag
     2401 acctctccat actgatcatg ggttgtacgt gccaattgcc ctttggcttg tttccgattc
     2461 tcaggaaaaa tcggtgtacc tgtaaagcca aaccaagtgg atttcggaaa catttttttg
     2521 atttcttcca tattttcagc acttaacgct cgatgacact catctactac aaacacgata
     2581 tgttggccca ttaatttttg gaagcgattg gttccttttt tctcttcttg tttctttgca
     2641 tatttcaacg ccgcatctag tttttgacgg gttgtaatga ttacaacatt tgcattggca
     2701 tctgccaaca gggtttcact taattctttg gcacttcccg ttccaacgat caagctattc
     2761 gctttggcgt tcccggaaga aatcccagta ttaaattccg aagcaaattt tgtaaactct
     2821 gtggtggttt gattatctaa atctttgcgg tccactaaca tgatcgttcg atcgatcccc
     2881 gattttcgtg ctaaaagctt agtagataca aaactagtca acgtctttcc tgaaccagtc
     2941 gcatgccaca cataccctga ttgatgttta tttgcggcaa taaacaaggc ttgaatcgca
     3001 tgtacttgat aaggatgtaa caccatgagg gttttattat cttgatcttc actgacgatt
     3061 gtataatccg caatcaaacg atgagcatcc gggatattta acacttgttt acaaaactca
     3121 tataagtttt ctactttttc attgtctttc gttcgccaac taaataaaaa cttagggtgc
     3181 aagtctttcg gtaaggcatt tgcaaaatac cgagtggttt gttcattgga aaccacaaac
     3241 aactgtaagg tcgaaaagat attgttccga aacatacctt cttctgcata ttttttgatt
     3301 tggttaaatg cctgataaac accatctttt gcagttactt gtttcagctc gatttggaca
     3361 atcggtaatc cattgattaa taaggtgacg tcaaaacgtc gatcccgtgc ttctatgtta
     3421 cttccacgtt ttgcaatttg atggaccact tcataagtag agattcctcc accaatgtct
     3481 tgattggaat aaaggataag tgagacagaa cccaattgcg aatcttcccg ttcaatcgtg
     3541 atccgagcca tcccattttc accttttaac catttcgcag catcaaatgg ggttttcgtt
     3601 cgcagcagta attccgtttt gatcgtttca aattctttat cggtcaaagg aaaatcattc
     3661 agttctgctt gattatttga gataattttt tggcgtaaat tgacccaaag gtcttcttct
     3721 gattttagat ccggacgata cgtccactga ttatgccctt ctccaagtac acgaatcaac
     3781 tgttcttcga cttctgcttc ctcacggtga ctaattttca ttcttcttcc ctcctagaca
     3841 aacatctttt gtaagaagct ttttttcgtt tcttttagta aatctaactt acgttgatga
     3901 agagcgatcg tatcatctag ctgtttgaag aaagtaccga ttttttgttg ttcatccttt
     3961 acaggaacct taacactgat attctcaata tcagaactgt tataagaagg caatgcccct
     4021 acttgggagt atcttggcaa atttatagtt tcaaacaata ttttaccaaa atagatatcc
     4081 caagtattat taaataaata agccatggta ttgttatcta ttaagaaagg gcttgtgacc
     4141 aaacgttttc tattaagcat tattgctgcg ccaactttag caaatataat cgctggcaca
     4201 tcttttattg gcttccattt tttacgctca atttgctcat tagatacata atgatttgca
     4261 tttctcattt catactcatt accaatgtta ttcatgtcgg aaactttaaa aaatggagta
     4321 ccttcagaac caccttgctc ggcatcagga aatccaattc ccgattgagt tttacctaat
     4381 tctcccaact tacgctcttc ccaatcttcc gtaaaaccag gaaaccggat ttctggcact
     4441 tttgctccgt tttcagggaa cattttttgt aagaatcctt ttttcgtttc cttcagcaaa
     4501 tctaacttac gttgatgaag agcgatagtg tcgtcgagtt ttttgaagaa tatgccgatt
     4561 ttttgttgtt cttttaaatc attgggaata tagaatactg tatctgaaac ttttttccag
     4621 tctgaacgag gcatttttgt tccagatgta tcatttgcag ccttttgata tctatcagct
     4681 tgaataagag aataaataaa gtccgagtcg ctcttcttag ctctaaaaac ccagaaatcc
     4741 ccaatcgcta cgcctgtaaa agttgccttg agccaatttt ttaaatatgg acgtaacttt
     4801 ccatatagaa tatcatttgg taaaaattca atcccttttc tattatcaaa tttctggctc
     4861 acatctttat tcagtctacc ttctcctgcc acgatatctt caaattcaac ttttggaagt
     4921 ctactggagt ttgtactttt attaagtcta acaactaaat caattaactt acgctcttcc
     4981 caatcttccg taaatcctgg gaaacggatt tctggtcgtg tatcattgct catgtttgaa
     5041 cacctccagc gcaccttgta accacatttc gttttcaggt gttgtttgca gggaagagat
     5101 agcttctaaa agttcttttt ctagcacttg ctgttcttgt cgtatcttct tgatgtcatt
     5161 tccgacatga accatatcaa ttggttcttc ttcttcgaaa gtatctacat agcgtgggat
     5221 atttaagtta tagtcatttt ctttgatttc atcaaaggtt gccaaatgag cgtatttctc
     5281 gacatctttt ctttcagcgt aggtttcgag gattttttga atgttttctt ccgatagttt
     5341 attttgattt ttccctttaa caaattcacg actagcatca atgaataata catctcgagt
     5401 ttgacgattc ttcttcaaca caatcaccgt cgttggaatc gatgtcccaa agaacaagtt
     5461 cgcaggcata ccgatcacag cataaatact gccatcttct agtaattttt gacggatgac
     5521 cccttctgca gcgccacgga ataaaactcc gtgtggtaag acgatggcca tggtacctgt
     5581 ttccttcaaa tgatagaacc catgaagaag aaacgcaaaa tctgctttgg attttggcgc
     5641 taatttccca taacgattaa accgagaatc atctaagaaa gtcgtatctg ctgaccaatt
     5701 agccgaataa ggtgggttca ttactactgc atcaaatgtg taaggttcat ctgttggcca
     5761 gtccttattc aacgtatccc cattgcggag attcatttct tcggcgtcga caccatgtaa
     5821 aatcaagttc atttttgcta gattgtatgt cgttgtattt agttcttgcc catgatattt
     5881 aacgttatct gggtgtgtta agtagttacg tacgtttaac atcaatgaac ctgaacccat
     5941 cgttggatca aaaacactaa agaatcgtcg ttccttttga tcgagcgtta cgatttgcgc
     6001 catcatatca gaaaccatat gaggtgtgta aaattctccg gctttcttcc ctgcttctga
     6061 agcaaattga ctgatcagaa actcatacgc atccccgatc acgtccccat cgtgttctaa
     6121 aacatctaca tcattcaatt tcttgatgac ttcggtaatc gtcacatttc gttgttgctc
     6181 atctgttcct agtttttttg attggagatc cacatcatca aataacccat taaattgatt
     6241 gtaggtagat gccaattgca caaaggcttt gtttagatcg ttcaattgga acgttgcttg
     6301 ttttgcttga tccgctaaga cgttgaaaag atactctggt gcaattgcat atcccaaaat
     6361 atcaacgatt gtggcaatca gatcttcttt tgattcttca tctgaaagta attcacgata
     6421 caacattgtt tgtttgctta ctgtgtcata ttcctctaag gattcatctg ctaaaagaac
     6481 cacttgttcc aacaatcgat cggataaata tttatagaaa atcagtccca ataaataatt
     6541 tttatattct gacgcatcca ttttactacg gagattatct gccgcactaa ataatttttg
     6601 atttaattct gcacccaaaa agatacactc ctatctctta tttaaagttg ctatataccc
     6661 tcttatttta gcataccccc ttttataaaa agcaaaaaaa aaagaaagaa acttattctt
     6721 gctaataaac tacgcgaaag gagtctaacg ttgtttgata taacttagac tgatcgttaa
     6781 cttatttttt atatttctac atagttatct ttagaaccat ttcagataca tatgaaacaa
     6841 ttacatatga ttttttcata aaaaaacagt actgtctagt agtataaaat atacaaattt
     6901 cttaaaaatt ttttcactaa aatcactacc tgaaacagtc taaaacactt atatatcaac
     6961 gtttaatgaa actatctccg tttcatttca gatacaaata aatatagtcg ggctccaaat
     7021 ccgaaaaaaa atacccccag tagcttatcc taagctactg gagatgtata agtacggtca
     7081 tataacaacg gggtagggta aataaatgaa aaagatggaa gaatcattat tcaataactt
     7141 ttttaaatac ttcgttgtaa tcagttacct gtccgttctt aaataccaca gaataatctt
     7201 tttacccatt ttcaagtaac cgaagtggaa tgtctttacc aacttgctct gtcactgcat
     7261 tactgatacc tgttagatta gctaataaat ctgataaaga ttgagcatct gtagaaatct
     7321 ctgtatcagt atttatcgtg attcctttat cttcatttac aagcttccaa gtaggtggta
     7381 tagctgtgta tgtttcgatg aatgaatcaa ttctttcatc tatcgtcttc ccagtatcta
     7441 cttctgtctc attactctta gatgaatcga ttctttcatc catcgtctgc tccgtaactg
     7501 cttctgtctc attattctca ccggaaactc tcttcacacc caaacttata tattgtccat
     7561 ctttagtgtg catagttaaa attcccacgc ttttactaat aaattcacta taaccagttc
     7621 tttcaataga gacttcaatg tatttcacac tatcatactc aaaagaaaaa tagacatgta
     7681 aaagcatttt gttgtttaca tctacataat aatcctttga ctctacttga gaccttccta
     7741 tacggaatac agctttactg tctgatatct ctataccagc atccttatta ttaatctgcc
     7801 aatctccaag tagctcttct ggcaagctag tagtttcttg tgaacacttt accacttcac
     7861 tggtagaggt cgaaacattc acctccgcac tgttagaaga attttccaag gaatcaggag
     7921 ccttagcaca accaacaagt ctttatatat tgtgcagccg atattttctt cattcagtac
     7981 acccttgaaa tatgcagtgt tagataaagc agttcgtcct gatttgctga ggcacccatc
     8041 tcttcttgaa cgtactcctt gattttcaag gccgcccgat actctttcgg aaaacgctgc
     8101 ttcactgaag ccagcaaaaa atcatccttc tccggcaact cttccttctt aatcattcgg
     8161 gcaataaaaa actgtaaatg cgtcacaaat cttaagtaaa gcatcgaatc tttatcaacc
     8221 tttttttcgg gaaacagata tcctagcata gccaagatat cctttagtgc tcttaccgag
     8281 agcatggttt cttccatatt tgcttgttca ctttgggcat ttacaatgtg aaacgcaatg
     8341 tttccagcct cctcttctgg caactgaaca ttcatttttt tgttgatcaa ttggatagca
     8401 tagcagccga tctcaaattc ttttgggtaa tacttttgaa tttcaatcag caggcgattt
     8461 tgaaaaacaa tgcctttttg actgcgttca atggcatgct tcaagtgatc aaccaaggta
     8521 aaaaatagac ttttattcag cttaaagtca aacttggcgt tggcataatc gataattcgc
     8581 ttgacgcaaa taattagatc atccggagca gcctcaaaca gtttaatgta atcctttttt
     8641 agagcatcag aaagtgaatc taatttaaat actttttgga ttttgtcagc cggaaccaaa
     8701 tcgcctttct ttagttgaaa tccaatccct ttccccatca aaattacttc tttgccttgc
     8761 ttatctaagc aaagaattaa actgttgttt aacgttttaa ttactctcat tagattcacc
     8821 gttctctcta agctaagcca tttcttgcga ttactttttg ataccaaaag aagctttctt
     8881 tttttattcg ttttaattct ttaagattct catcggttcg gtcaacatat ataaaaccat
     8941 aacgcttatt gaagccttga tgggtactaa caacatcaat cgctgaccaa ggtaaataac
     9001 caaaaacatc tacaccatct gttaaagctg cttgaatagc cagaatatgt ttggctagat
     9061 aatcaattcg atcctggtca ttaattcgtc tatcttcctc aactatgtcg ggtgccccat
     9121 agccattttc agtaattaaa ataggtaaat catagcgatc gtacaatttc ctcaatgtta
     9181 atcgtagtcc agtagggtca actacccagc catatggcgt tttatctaca taaggatttt
     9241 cagctgcccg gtagacacct tcttctccca acatgatctg ttgatccccc gctctagcgg
     9301 aaacatctga tccgtcattt ttgctggcag cgatggtggc agttgaataa taattaatcg
     9361 caataaaatc gggtttaccg gaggataaaa ttgcctgatc ctctgcctca attttaggat
     9421 ataatccacg atcattcaaa tagttttcaa acaaataatt gtattttccc cgaactgcta
     9481 catctaagaa agaccagcct ctcaatgttt cccaattatg ggctgcaatt gcatcttctg
     9541 gactggaggt agcttgatac atagccgtta aattgattgc tggaccgatt tttcctttaa
     9601 gattcaactg atgatacaag tgaattactc tagcttgagc taataaaaca tggtgattaa
     9661 tttgaaagag ttcttttttt gaaggcagct ctccacttgg tggcaaacca atcgctccag
     9721 gatgaaggat catcgtgttt tgctcattaa ttgtcagcca atagttcacc ttgtcaccat
     9781 actcagtaag caatacctgt acatagtcca caaaataatc gatagttgct cgatttaacc
     9841 aaccgccctg ctcctctaga aaataaggta aatcgaaatg gtacatggtg acaatcggtt
     9901 cgattccctg attcaggcat tcatcaatta cttgatgata aaaagaaatt cccttttgat
     9961 tggttttatt attttcaata tccataattc gcgtccaagc gatggaaaag cgaaaagctt
    10021 ttaagcctaa ttctttaaac agctgtatat cttctttata gtgatggtaa aaatccgagg
    10081 caacttcaaa attagctacc ccttctgtat gctgatactt gtcaataata gacggttgtt
    10141 tcccatcctc ctgataggca ccctctgcct ggtaggcact aatggaggca ccccataaaa
    10201 acttctctgg aaatgctgaa agctgtttgt attgcacaaa aatcctctcc ttaactaatt
    10261 tgcatcaatg gttcgtgttc ctttaagctt ccgcttgctt cactaattcc tgcgtaattt
    10321 tcactgttgg tcactaccac aatcactgta tcatcgtagc cagcgtttct aatggcagcg
    10381 aaatcggcgt tgattaataa ttgtcctgct gacacctgat ctcctgcttt tacaaatact
    10441 tcaaagggtt ccccttttaa attcacggtg tcgatcccca catggatcaa taattcaata
    10501 ccttctttag aacgtagacc aatcgcatgt ttagattcta aaatagtaat gacttctccc
    10561 tcaacaggtg aataaatgct gttttcttct ggatgaatgg ctacgccctt tcccaatagt
    10621 cccttagaaa agacagcatc gttcacttca gaaataggac acacggttcc ttttaccgga
    10681 ttttcaacaa tgactgcttc ggctattgcc gtttgctctg attcatcaga agtatcgtca
    10741 aatccaatta gataagcaat gataaaggtt gcaacaaagg cgattacaca cgtaataatt
    10801 gcgtgaacaa tattcattgg attttctcca ataaaggctg gtaaggctgc taagccaggg
    10861 gaaacaaagg catatctgac taaaccgctc aagccagcat acaaaccagc aattgcacca
    10921 ccaatcatgg aagcaactaa tggttttttc agcttcaagg ttactccata gagtgaaggc
    10981 tcagtaattc ccattaaagc agtgatccca gtggacacag ctagctgctt caactctttg
    11041 tttttagttt tcgcggctac agcaaaggta gcacctgatt gagcaatatt agaagccagc
    11101 attcctggcc cattgatcat ttcagaaccc gtactgctca attgcatagt agcgattggc
    11161 gtcattgccc aagctgtacc cgtaaccacc aaaaatggct gcaaaccacc catcaaaaat
    11221 ggaattaacc aagctgcttt gccgttaatc cattcagccc ccattgctat aagatcgttc
    11281 agcaacgttc caaacggtcc aatgacaacc aacgctaaag gccccataat taaaaatacc
    11341 agcatcggct tagtaaagaa tttaataaac gaaggcgaaa ttcgatccgc aaagggctct
    11401 acataggaca tgatccaaac agtaaagata atcggtaaaa ctgatcccgc ataatctgca
    11461 gctaataccg gaattcctag aaaagaaacg gcatcgccag tagaaaaaag ctgtgaaata
    11521 tttggatgaa gcaaaatgcc agcaacagta atcgccaaaa ttgggttgac atcaaacttt
    11581 atggctgccc cataggctaa tagtactggc ataaaataaa aggctgcatc agcaatagta
    11641 ttcaaaatga tgtaagtttg actactgtct gatagtacac cagtcaaagt caagacggcc
    11701 aaaatggctt ttaccatccc ggcacctgtt atagctggaa tcatcggtgt aaaggtggta
    11761 gaaatgacac taatcaatct tgaaataata ctttcttgat tgtctgaaga ggggcccttt
    11821 ttaattgaag ttcccacttc cttttgaatt tccttgaagg ctgtttggac ttcattgcca
    11881 atcaccactt ggtattgtcc accgctatcc accacactaa ttacaccaag tgttttttct
    11941 aaggctttcg tatcggcttt ccttttatct ttgagttcaa aacgcagtct ggtggcacaa
    12001 tgggttaagc tgaccacatt ttcttttccc ccaacctgtt gcaatacatt tcttcctact
    12061 tctctgtaat ccattattct ctctccttaa agcagtaaaa aagaccaagt caaaccacgc
    12121 cccaatcaac attgaagtgt tagtttgtct tggtcttgcc tgcataatca gtaacatgcc
    12181 aataacgtta ttttgctgta atccaataat agcacattat gatagcgcaa tcaacaggga
    12241 atttaatttt ttatcttttc gtttgaccgt atagggtaat ataacagtta gatcacatca
    12301 actagggtat atctgcgtat acttactgct aaaataagta tatctaatat taactatacc
    12361 agtaaaacca ttcctacata accagcggga aattcccaaa atcgtgaaag catcaaaaat
    12421 gcatataacg gatcacacaa cttcaacaat tctgctcttg ggaaaaattg atgaatttgg
    12481 tgagtaaagg atggaataaa cagcattcca acaatatagg caacaatagt gatacttgtt
    12541 tttttggtaa aaatcagtaa gcaatagcca atttcaatca ttccgagata taaaggtatc
    12601 aatagaatga tttgggctag tattcttaaa aattaactag caaatgttta aactgattta
    12661 tttctaatct acatgtagta agtatttcaa ttccaccaat tatcaataaa aataccaaac
    12721 atatccataa taattttttc tttttcattt cttttatatt gttataacag tgaataacat
    12781 aaaaattccc gacaatcatt gtaatatttt tttgtcacct gtattagtat ttaatttata
    12841 ggaaactata aaaactccct agtgaattta cacctttggg agttcctgtt tggacggtat
    12901 gcataataca tttaaatcaa tttaacatta acacctcagc atatggtatc tccacatata
    12961 tacggtcttt ctttggatag tactcaatat ttatgttgat agcacttcca ttgtctaata
    13021 tagctttagc ccggtatgtg ttatctccat tttcatgagt aatcgtcaca tcatcacatg
    13081 atcgatttaa gttgtactgt tcttgtaaaa tctgtgttac caatggtttt gatgacttct
    13141 ctaatccaga tgtacctgtc atttttgaaa aaataatacc tataatagcc aagacaacaa
    13201 tacctataat gacaaacttc ttgttcatat ttactttatt caacttttta tctcctatct
    13261 ccaaaataat ttgttcttat ccctcttatc agtacattga catccctaaa agtacaccaa
    13321 agattgctcc agctacccct gctatcatca aaatcgtacc cattttctca tctttttctc
    13381 gatataagta gcccataata acagcaacag caccaaatac aacaggaata aatgctaacg
    13441 atactaccat agaaatccaa ccaataatca aatacagctt ccctgtctct tgtccatgta
    13501 cgatttgttc cttcatttca ttaattgtct gctcagtact tttctttact gtgtctgttc
    13561 cggttgtctc atctgtttct tgtagtgctc cacaaaactg gcaatactta ctttcatctg
    13621 ttatttcttt gccacatttt ctacaataca tgtcttctcc tcctaaaatc attcaattgc
    13681 ttattagtta actgtttact ttattaaata catcagaaaa cagctcattc attggtacac
    13741 ttaatatctc agaaagcatc ttcatttgat agggtttagg aatcgttctc cctgtttgcc
    13801 acgaacttac acattgctgt gtaacattca ttttttttgc taactccgtt tgagaaagtc
    13861 ctttttcttt tctgtatcgg ctgagattac ttactttttt catgattctc ccctcctgtt
    13921 ttattatata caacaatatg atgtatttca actcataaaa tataaaaaac ttcaaatacc
    13981 attacaaata caacaacttg ttgtactata ctgagatggg ggtgtattaa tgaaacagaa
    14041 acaatatgca aaggtcatta gagaaaagcg aaaagctctc gggtggacac aaaaagaatt
    14101 agctgaaaaa atcttctcta cacaacaggc agtagcaaga tgggaaaatt ctgtaacaga
    14161 gcctaacctc gatagcttaa cagctctttc tagagcccta ggaacccctg taagccattt
    14221 tctagacaat gttgtcgtag attatgagga agagtttttg gctctctatc gttctcttag
    14281 cactgaagat gcagttcgaa caattgacta tatgaagcta ttaaaaaggc aagaaaatga
    14341 acgcaatcag ctattgaaag attgaaaaat aaataaccca ccgaacttta acggtgggtg
    14401 tattgagatg aaataagatt aagagctatg gataacctgt ctagtttaat acaattgggt
    14461 cttttctctt ccttactctc cgtccatcat ctcttgttac acttgtaaat tgatacagag
    14521 gaactatccc agctcctgat aatatcaatt cttttattgt ttcatctatc cctgcccctt
    14581 tcgctaaata tattaaaaaa ctactacttg ctttcacatt gtatagtttc ttaatatcct
    14641 tagaagctcg ttcaagagtg aatccttgtt taacaaagta gcctctgttt tttattaaag
    14701 catttttcac gctgttatcc ccaacattac gtaggcgttc acaaacagtg ttaataagtt
    14761 ctttctctgt cattagatat gtcccttgcc acgataagcc ctgtaaaact tcatgtgctt
    14821 catctaaaat caattgtttt tccttgctga gttctgatcg ggaattttga ggaaatactc
    14881 ttttagcctc tttttcgtta aaacatatct taacccattc ataaga
//
LOCUS       DAZKNO010000059        14677 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000059 DAZKNO010000000
VERSION     DAZKNO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.060"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(291..632)
                     /locus_tag="ACG8MY_001362"
     CDS             complement(291..632)
                     /locus_tag="ACG8MY_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0430559.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(632..862)
                     /locus_tag="ACG8MY_001363"
     CDS             complement(632..862)
                     /locus_tag="ACG8MY_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0430560.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1149..1487
                     /locus_tag="ACG8MY_001364"
     CDS             1149..1487
                     /locus_tag="ACG8MY_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430561.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1692..2254)
                     /locus_tag="ACG8MY_001365"
                     /pseudo
     CDS             complement(1692..2254)
                     /locus_tag="ACG8MY_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(2986..3567)
                     /locus_tag="ACG8MY_001366"
     CDS             complement(2986..3567)
                     /locus_tag="ACG8MY_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0430562.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3750..4376)
                     /locus_tag="ACG8MY_001367"
     CDS             complement(3750..4376)
                     /locus_tag="ACG8MY_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0430563.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4398..5729)
                     /locus_tag="ACG8MY_001368"
     CDS             complement(4398..5729)
                     /locus_tag="ACG8MY_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0430564.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5884..6285)
                     /locus_tag="ACG8MY_001369"
     CDS             complement(5884..6285)
                     /locus_tag="ACG8MY_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0430565.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6371..6577)
                     /locus_tag="ACG8MY_001370"
     CDS             complement(6371..6577)
                     /locus_tag="ACG8MY_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430566.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6672..8174)
                     /locus_tag="ACG8MY_001371"
     CDS             complement(6672..8174)
                     /locus_tag="ACG8MY_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0430567.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8192..8647)
                     /gene="argR"
                     /locus_tag="ACG8MY_001372"
     CDS             complement(8192..8647)
                     /gene="argR"
                     /locus_tag="ACG8MY_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0430568.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8745..9362)
                     /locus_tag="ACG8MY_001373"
     CDS             complement(8745..9362)
                     /locus_tag="ACG8MY_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0430569.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9583..10956
                     /locus_tag="ACG8MY_001374"
     CDS             9583..10956
                     /locus_tag="ACG8MY_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0430570.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11031..12143
                     /locus_tag="ACG8MY_001375"
     CDS             11031..12143
                     /locus_tag="ACG8MY_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0430571.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12215..13852)
                     /locus_tag="ACG8MY_001376"
     CDS             complement(12215..13852)
                     /locus_tag="ACG8MY_001376"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0430572.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14020..14628)
                     /locus_tag="ACG8MY_001377"
     CDS             complement(14020..14628)
                     /locus_tag="ACG8MY_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0430573.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4000 a         3054 c         2587 g         5036 t
ORIGIN      
        1 ttaggactaa aactgtcagt tgttcttttt cttctttctg ttaaagtatt gtcgcgactt
       61 ttcgtatacg ttaagatatg tcgtgaaaaa acaagttttg tgacacaaaa aaacgaatga
      121 cttgaggaaa tcacctcaat cattcgttta aaatgctata tattctttag tttaagatac
      181 ggaaattaat gccatcgacc acactagtag aatgaacgac atttacttgg ccttttttta
      241 atttcgttat gtttagcata taaaaatatt atagagttta cttacaatgc ttaaaacata
      301 tactgtataa tctgatcaat tttagccatg tcttgcaatg gtaagttttc tatttttttg
      361 agttttcgtt ccttaaaatc aagagatttt aattgtgtaa taattacttg tccttttata
      421 tctagctctt caggtaatga gtaacgtgtc ggtaaattct ttatagtgga agtaattgga
      481 catatgaccg aaaacattgt tttacggtta aattcataac gagagacgac tagtcctggt
      541 cgtctttttt gaatttcttt tcctacggag ggatcaaaat caatccatac aatatcacct
      601 tttttaggaa tataaattct ttcttctgac actataaaat ttctctccct tcaggagtta
      661 aatcttccca ttcgtctttc tcataatact cggcctcttc cccaccacta aatggatctt
      721 caatttttgg aactaacgta attgttccat catcagaata cacaacgata tattcttggt
      781 tttctgaagg tttctttccg ttatcagaag gtaaagttac cactacagaa cttccttgta
      841 atctagattt tgtaacaagc attatcatca ctcccttaat aaaagtatac tatataacac
      901 taagtaacgc aaaattcccc tttgtaaact atctagaaaa ttgttttgtt taaagtatat
      961 cttattggat ataaatagaa ataaattgta gccagttcat tttttcttta actaaaaaaa
     1021 taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat agctaacccc
     1081 ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag acaacaggcc
     1141 atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct gacaaatttc
     1201 aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg atcaaagaat
     1261 gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac tttgctattg
     1321 tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat ccaactcata
     1381 tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca actgccaacc
     1441 agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga taaacatcgt
     1501 taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc tatacattag
     1561 aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt tcttctgttc
     1621 tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct ttctttgaaa
     1681 aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt aatgattttt
     1741 cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa gacaagcggc
     1801 attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat gattgattct
     1861 gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat tttttcctta
     1921 tttgaaaaca taaatttgtc tttttcttca ttccactcat tgggaaaaca caacgatttt
     1981 gcaaaaatgg ttgatgaaag gtcttttttt cttctactgt ctctgcacgg gcattaatca
     2041 tcaattgtcc ttttttaaat ccggtaaatc cccacttcat gatttccgga gtgaggatgt
     2101 tttcttcctc ttttcttaac gtcacaacaa gatttgacgg aaaaacttct ccagctgcta
     2161 attctgctgc ctgcttcggt tgtttcgtct gggcttctcg ataataattc tgaaggagct
     2221 ctgactctag atcaaaaaaa taacgaccac acatcctaat ccctcccaat ctttttttct
     2281 atcatacact gcaacaagaa gttcgcacca acaaaacgct ttcaattttt gtagaactaa
     2341 aaaagttgtg acaagtgaca aactctccag tgaatggtat tctcctgcca actcctcggt
     2401 cacaagtcac aatattcaac aaacatgaga agctgctttt tgaaattgtt ccttgtgact
     2461 cggagtgaaa cggcaggctt acgacctctg ggtaaacggt gttcaaaagc cagtttctcg
     2521 gtattaagcc aaatttatgc aaaaatgtag aaagctattt tcacaaaatt tttcttaata
     2581 ctcgaaacta agcgactttt tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
     2641 tgcggtaata agcccaacca aacgaaaaat atgagcagct atttttattt ggctgttctt
     2701 attacttgat gcagggcagc tttttcacaa cctctccact aaacgatgtt caatcagcta
     2761 accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct
     2821 ctaatactca ggtcaaagcg ctgatttcac aacctctaat acacggtgtt ccagaagtag
     2881 cttcttcgaa aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc
     2941 ttcttatttc ttgaagctga ccacttctgt cacaacctct aattattaaa gaaccttttg
     3001 cgaagttaac aatctttctt tgatcaattg aaaattctgc ctaagtagtg atagatcctg
     3061 tgtgactttc aacaataaga tacttccctg gatttcagca atcaacgctt tggcttgacg
     3121 gtctgcttct tcatcggaca gtttttcttc agaaagtgcc aggatatttt gtttcgtata
     3181 cttgacccat tgattgacta attctagtag caacagacgg tattcttcat ccactgtact
     3241 caactcaata atcaaattac ctacaggaca gccatgataa gtgatctgtg tctcatgatc
     3301 agcgaaaatc cagtccaaca tttcttctaa tgcttcttct ggctgttttc cagcatttaa
     3361 gatgtcttcg aacaattctg tctgccatac gtccatatgc gcatggatca ccgctagaca
     3421 aacttctttt tttgaagaaa agtaataata aaattgacct tttcctgtat cagaagctgc
     3481 tagaatatct cggattgacg tttcatgata gcctttttta taaataagat ctttggtcgc
     3541 ttccaagatc ttttcctttg ttttcaatga tattctcctt tttcatttct catagtccta
     3601 tattatacag taaattcatc attttttctt cttaatcgtt taaaaaagac tgtgccaaat
     3661 tatctgacac agccctcact ataaaactgt tctcatatag ctagatgttc attgattcaa
     3721 gaactgcgag gacaaaagca attcctatct tatgatagtt gctctaatac ttcctctgtt
     3781 gtcatcactt gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat tgcttcttgg
     3841 ccaggagcac caattgcatc tgtcgctacc acaacattga atccactctc taccaattca
     3901 cgcatatggg aatcgctaca caagtttccg ttcattccac caagaattac tgtgtcgatt
     3961 ccattttttc tcaattggag cgccaaatca ttgctttctg gtccaaagat tttatgagga
     4021 gagctgatga tcgtattttc atccaaataa ggtttcaatt cttcgataaa atcggctcct
     4081 gaaccttctt ccactgattc atattgactt tttctagcaa acatctgctg ctcaagcatc
     4141 atatgttctc ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa ataatgagga
     4201 gaaataaaga gtttgtatcc tttttctttt gcttgtttta ccatttcaat catattttcg
     4261 atcgtattgt atttttctaa gatatctttt gtcaaaccaa agccaccgcc tgctggtttc
     4321 aagaactcga cttgcgggtc agtgagaact actgctgtat ttttattatt gaacatggtg
     4381 gattctccat tcgttattta ttttaataca tcatttagag cttcactcat tgtcggatga
     4441 gaatagattt gatctctcaa taacgtataa ggaagttttc cattcatagc tagcgaaatc
     4501 aagttgatga cttcatgaga atcttccgca tagatacttg cccctagaat caagtcagtt
     4561 tcagggtcga ctaaaacttt gaatacacct ttaggatctt ctaatacttg tgctttaggg
     4621 acaccgcttg tcatatactt gaataatttg taattgacgc cttgtgcttt ggcttgtttt
     4681 tctgttaaac ctacattgga taaagtcgga gtgataaata cactgtaagg cacagctttc
     4741 cggttattcg ttgttcgttt attctcgccc ttcaactgat ccataatgat tcgataatca
     4801 tctagagaga tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc ccaaatgttg
     4861 tctgcatctg tacgaaggta ttcatccaca gcgattgctc cgcgatctgt tagtttaaca
     4921 tctgtatttt ctaatccaag tgatgctgta cttggttttc ttccagttgc agccaaaact
     4981 tttgacgctt ggattgttac ttcttggcca tcttctgtaa aggtgacggc agctgttgat
     5041 tcttggtctg ctacttgatc tacagataca cctagatgga attcgattcc cgcatttgtc
     5101 atgtcttcta aaatcatttg cgcgatatct tcatcttcac gtggaagaaa ttcagaatgg
     5161 gcatcaagaa cgacaacttt agatccgtat tcattgaaca ttgatgcaaa ttccaagcca
     5221 atatatccag ccccgatgat cactaatttt tccggtaatt gttcctgatc catcgcttgc
     5281 gtagaatcaa tcaagtagcg acttgtattc aatcctttga ttggtaaaat gattggttca
     5341 gaaccagtat tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc attattcgtc
     5401 acttcgattt gatggttaga aagaaaacgt gctgtaccgt ccatcactgt cccagttgcc
     5461 tcatcgctga tcatatgata atttttggcg cgaagcatgc ccgtcaattt ttctttccga
     5521 gcaacagcct ctgtaaagtc gattcctctt tctccattga aaatcaatga tttagaagga
     5581 atacagccga tgttgataca tgttccgcca tacatacgtg ttgacttttc aacgactaaa
     5641 accgattcac cttttgaagc taacgccttc gctaaagtct taccgccctt tccaaaaccg
     5701 atcacaatat tttgatattt ttccatcatt tttccctcgc ttctttttta cctaccgatt
     5761 ggtacaaaaa tatgataacc gataattgtc tattctccaa atcatctgct ttatttgtca
     5821 aaaaaaacca gacaaaaagc ggtcctttca cgtttttagt ctggttcgat ttcctttatt
     5881 cttctaaaca atcgttactt ctacatcgat atttcctcta gttgctttgg agtaagggca
     5941 gaattcatgc gcatcttctg caattttttg ggcttcttct tcgctgatgc catcgatatg
     6001 cacttctaat actacaccga tctgatagcc gccatcatct aaattataaa gagagacacg
     6061 tgcttttact gtggactctg ctgtcattcc tgcttctttg atcatatgtt gtaccgcccc
     6121 attgaaacaa gaagcataac ctgctgcaaa taattgttct ggatttgttc cttctgcatg
     6181 gattcctgga ggagtgattt tcatatgcat cgagccattt ggtgcctcga cttctccatt
     6241 gcgcccgcca tggttgatca ttgctgtttc gtatgctttt ttcattgtac tgctcctttc
     6301 gattgaccat ttgctacttg gctataatta tagtgtatga caagtaaggc gctttctaca
     6361 aaaattctgc ttattcgaat accagaatgc cgtcttctac tatttttcca aaaaacaaaa
     6421 aaatactact cgtgaacaac agcgtcatat ttgttccaaa ataaatagca atcttttcta
     6481 cataatcttt tatatgattg attttaagat acatatatac cagaaagttg accccataaa
     6541 ccaatactgt taaaaaaata accatattcg ttaccatttt ttcctccttt tgctggtctc
     6601 cctaccagca ctcgatttaa ccataccact ttttttgccg tccaagcaac tagcctttca
     6661 aaaagactgg tttattctgc tgttaatgat tcaagatcag aaaaatgtcc gaataatttt
     6721 gctaatttta ctaacgccaa cttgttcttt ccgaattcat cattttctat ctgtaatact
     6781 agtaacggct catgtaagct taaacggatc aaaaaccagc cgtttccgta tggctcttct
     6841 agatcatatc gaataccttc ttcattttcc gggtcgaagt tcatcccttt ttcttcttcc
     6901 aacagtcgct tcatttcttc taatatcttg tttccttgtt cgcgtggttc atcgctgaac
     6961 aattttaatc ggatttcttg tacttctacg ggctgtttca actctttgat ttgttcatct
     7021 aatgtgatac cttttctttt taattctggc aaaagcatca ggatctttgc gatgacatat
     7081 gcaccatcat ccagaaaata gttttccttg aatgcagcgt gtccgcttgt ttcgatggct
     7141 agctgtgtat ctttcccaag acggttaagt tctaatgctt tattgattac atttcgatag
     7201 ccggaaatat aacgaacttg gatgcctcct ttttcttcaa tgaatctttg tagatgagaa
     7261 gaagtagggg aattcgtcac gattcttgtt cctggatgct cttttaatac gattgtactt
     7321 aatactgcga tcaaattatt tcgattgatc actgttccag aagcactgac aactgcagaa
     7381 cgatccacat ctgtatcgaa aatgatccca agatctgctt catttgctaa aacagcctgc
     7441 ttgatacttt ccatcgcttc tttattatca ggatttggga tatgattagg aaatcggcca
     7501 tctggttcca aaaattggga acctgttgaa tccgcaccta agctattcaa tactttttca
     7561 gcaaagaatc caccagcacc atttccggca tcaacaatga ttttccaacc atttagtggt
     7621 ttgctttcta gtgttccaat ccccttttta atttttgtga ctaaatcttg cgcataaggt
     7681 gtcaaaaggt ctgctttttc tacgcttcca gtttgttcgc tttttatttt ttgctgctgt
     7741 gataggataa aagcaatatc ttctttttct gctcctcctt ctgaggtaaa gaattttatt
     7801 ccattataat aatagggaag atggctagca gtcagcataa ccgcagcatc gcacgagaac
     7861 tgttcaaatt gagtggacat aaacatagca ggcgtagtag ctaattcgaa atcgatgaca
     7921 tgtatccctt gttctttcag actttggatc aacgcctctt tcagtgctgg tccgcttatc
     7981 ctactgtcat gaccaatccc aatcgtccat tttttatttg gatcggttga tttcttcttt
     8041 ttctttatcc agtttgctag tccccagccg atctttttta ccatgtctgt cgtcaagttt
     8101 acttcatatc cttcaactgg taatgcgatt cctcgaatat ccgatccatt ttgcagcgct
     8161 ttcatatctt tcatataaaa gtcctcctca tttattccct cattttttcc agcttttctg
     8221 caaatcgctg tgcttcttct tctgagcggc aaataataat gaatgtatct acaccggcca
     8281 tcgttccgac aacttcaggg taagccactt catctagcca attcgttacc acatcggcac
     8341 catttcgttc agtcaatacg ataaccatga actgaacaga ctggatacgc aaaacctcgc
     8401 gtctaattgc tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg ttaaataatg
     8461 tatagcgtac tattttgttt tcattttgac tttttactaa cttcatttct cgaatgtctc
     8521 gagaaatcgt tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca agcagctctt
     8581 cctgtgtccc aataggttgt tctgtaatga tctggcggat cagtgcttgt cgctgtttct
     8641 ttttcatatt agacacctct caatgttgat tatagcaaaa gataagtaat atttcttcat
     8701 attttggtat tttttacttt tattttttgt tttcattcag gttgctaacc tttttctatt
     8761 ttaacacgca aaatcgtctt cattttttca ggtgctgatt ttcttggatc gctgatgtaa
     8821 atctctttgt gttctttaga agctctttca tagccttctt ttgcgatgaa tgcttctaat
     8881 ttctcaaacg ttgctggttc ttcggcatag ggcccgacgt gtaagatttg ggcacaaaga
     8941 ccttcttcca tctttttgaa tactacttgt tctttcaagt cttccggaag tttgctagaa
     9001 cgctgaatgg cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg gatcatcaat
     9061 tgataagaaa aatgttcttt tttcatcact ggttcatgca aatacttttc ttgcagtccc
     9121 cattgtcctt ccagtggata gacagtatac atctgataat caggaatcag ccaatttttt
     9181 ttaggcgaca tgcgtattgc atagctgact gcatatagac aggctactct tcgtttaaag
     9241 tcttcctgat tcgggtcgcc gatccctctg atgctaaaga atgatttttt aggaacctct
     9301 agtatttgcg ggcttttttt catactatag ctcttgtctt tacgccattc aattttttcc
     9361 atttttcttc ctccgcgaaa ttccatttag ttatttatta tcatacctct ttttgagtga
     9421 cttggctaat cgtgttgaca ctatttttca gtttgtgcta gaataagtga agtaaataaa
     9481 tcgtacagat aacacgtttc ggcgtgtcga gagagaggcg gtttctattt ttataataga
     9541 aatcactctc tttttttacg aaatttttta gaggtgatgt aaatgacaaa acaaaaacag
     9601 ctgcttttag tacctgcagt aattgcaaca gggatgatgt cttttgcggg cgtattgatc
     9661 gagacggcca tgaatgtaac tttcccaact cttatcagac agtttgattt aacgactgca
     9721 caagtccaat gggtaacaac gatttatctt ttgatgattt cgatcattgt tccgctgtct
     9781 acttatttga atcgcaattt tagtctcaaa aaactctttt tagcttcaaa tctgcttttc
     9841 ttggcaggta taatgatcga tttcttctct cctaccttca tggtcctatt gtttggtcga
     9901 ttgctccaag gtgcagcgac aggtatcgct cttccgttga tgttccatat tatcttgaca
     9961 tttgcccccc tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac aacggccatt
    10021 gctccagcaa ttggccccac ttatggaggc attatgactt cacattttac atggaatcat
    10081 atcttcctgt ttcttgtgcc cgttttgatt ctttctttga ttatcggctg gtttgctatt
    10141 cccgagatgg cagtacaaaa aagtggttat ttggattgga taagcgtttt aggaatttgt
    10201 cttttgttta gtggaagtct aacatttttg agtacgttgg gaacagtttc cagctggatt
    10261 gctttaatca tcggcggtat tggctggttc cttttttact atcgaagtaa aaaagtggag
    10321 catccactga tccgactgtc tattctttca aataaaacat tccgcttgtt tttgattggc
    10381 ttcttagttt atcagttttt attacttggt gtatcttttg tcttgccgaa tttccttcag
    10441 atcgttcaag gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg cgcagcggtt
    10501 ggtgctttgc tttctccttt ttccgggaaa atgctggatc aatatggtcc gaagaagcca
    10561 atttttgctg ggctgattct ttctttgatt ggttggctag cattattcct actcatagga
    10621 cacgcttcag tgggtgtatt gattgcttgt catgtgtttt atatgatcgg tatcggatta
    10681 tcttacagca atatgatgac caccggaatg aatgcactga atgaagaact acaaggagac
    10741 gggaacgcaa tgttcaatac cttgcagcag ttttctggcg ctgtggctac ttcattagta
    10801 gcggtcatta tcaattacgt ccagcatcat acttcccata actatgaagt gtctactaca
    10861 ttaggatcaa aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag ctttgctcga
    10921 ttcgcctatt atctgttctt tgctaaaaaa gcgtagattc catatacggc aggtgttgca
    10981 tctgccgttt ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt atggaaaaaa
    11041 ttgccattat cggtggcggg atcattggta tgaccctagc cagttactta gatacatcaa
    11101 aattcaatgt tgctttattt gatgaaggga caggacaggc aactaaagct agcgctggga
    11161 ttatctcccc ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg gctaaagacg
    11221 gcgctgcttt tttccctaaa cttataaaag atttccaact tgatcaaacg atttacgcac
    11281 aatcagggac acttttgctt cgacctgaaa aagatttagc tgaattggct gttcttgccg
    11341 agaaacgaaa agaagaagcg ccagaaattg gaaacatcga atggctttca cctgaacaga
    11401 catctcaagc acttccgtta ttgaaaccag cagtttcact gcgtatcagt ggtggtggtc
    11461 gtctggatgg aaaagcatat ttagaacatc ttggtcagcg tttagcacaa aaacaaatgg
    11521 agcagatcca ggcaaaagtc actttgcata agaagagcga ttcttggctc atcaagggac
    11581 cgtttggaga atacacagct gatcgtgtaa ttttgacacc tggcccggca cttaagcctt
    11641 tattacaaga attaggctat gctgcagata tacgtccgca aaaagggcag ctcttagcat
    11701 ttcagacatc ttataagatg agtcaagact ggcctgttgc tatgcttgac ggagaagctg
    11761 atttcattcc tttcatggat ggaaagatcc ttttaggtgc tacccatgaa aacgatcaag
    11821 gctgggattt ggctcctact agcgaagctt ataagggatt gactaagagt acagaacagt
    11881 tcttgactga tccagatcag ataaaaaact ggccatacga gttccgtgta ggtacccgtg
    11941 cttatacgtc agatttttcc ccatttttcg gcgtactgcc agatgatcca agcttactgg
    12001 ctgctagcgg tctaggctct tctggcttga ccacaggtcc ttatatcggc tatctattag
    12061 ccaacttctt aaatactggt cattggccta cggaagatta tcaaaaggac atctccacct
    12121 atattacgaa aaatccatca tagaaaaaca gctttttcaa gtaaggatcc cgagcgatat
    12181 tgcgcatgga ttacttcttg aaaaagctgt tattttagtt caatttctga atcaaattct
    12241 ccaaactttc ttttgcttca tctggcaaga gatcttttcc tatctgagct tcaatgaatc
    12301 gtaaacgaat atcaagtcgt tgtttcacca gatttttata ggacgtatcc tcaaaagaaa
    12361 caggaaaatc tggtataggt aaatagtgga tgtcttcaaa tggaccaaac gacttgaatt
    12421 ctgccgtttg ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt ttgatttttt
    12481 tatcatcgaa atatccagta ttcaggacat agcaggctgt ttgctgtttc aaaaacaact
    12541 ctcgaaaagc ttgatagtct tccctaagag gatagacacg gaaaggatta gcaaatggtt
    12601 caatgactag ctcatctagc tgtctttttg tagtgatatt ttccgcgtct gatcgttttg
    12661 ttgctaatgt tgcaccaaaa gacgctgcta aaactgggtc attgatcttg ataattggcg
    12721 gaagcgaatc atctttcatg atccaaaaaa cagcatcgat cttttcgcta agatgatcga
    12781 ctcggtcggg tgtcgcataa cgtgatttga ctgtccgccc atttccgttt cgtatatctc
    12841 ctgttactag gactttttct cctttacttg tacatgtcac gcccacattc tgacaagtaa
    12901 ggaaagaaga aatggccgga tcatcagacg gatagtcttg tgttttatca aagtaagaag
    12961 gttccaaggc agtagcaaaa ccattttttc gagaaataac gaaagcatcg tcatgcaaga
    13021 tcgaaacaga gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt ccagaaccgg
    13081 acaacccaaa tacagccatc gtataggtct ggtcattact agcatattgt ttcatcccac
    13141 cgtggcaggc aataaagcca ttgcgatttc ccaatgccca agcaagcgtc aaagtcgctt
    13201 tttttaattc tccaaagtaa cgtaatccta gcacagccgc tgtattatgg agagcatcaa
    13261 acaaaatgag tccatttgga aatgtttcat gtgtccattc gggatcacaa aagatataaa
    13321 gatctccctc atcaaaggaa acagactttt gataagtctc taagatcttc gaattcgctg
    13381 gctgaaaatt cagcaaataa gaatacaaat tgttctcaaa tccttctgga atcagcaaat
    13441 gactttgcac agaaaattct tcagataggc caacgattac ttttgtatgg taaaactggc
    13501 gcttgcgact ttgatacaca gcttctctta aaattttctc gtatttgctt tgttccttgt
    13561 ctaagtcctc aaagatccgc cgagcacttg ctgttcttcc cacaatgctt ccatcattga
    13621 atactagtgt cctaacatca gctggtaaat ctagttcttc agcatgtgag attgttaaat
    13681 ctgtgatgat cgttcctgga ctcccttttg ctagttgata agctgtcttc ctatcaaaaa
    13741 cttcatgaac attattaccg taaaatgtcg tttctacaat cgatcgaata ctggataaca
    13801 gtggatttcc acttgtgatt tcgtttaatt cgagactctc ttttgtactc atggctcctc
    13861 ctcattccct aaacaaaaaa acgttttctt tattatacat tattcgagtt tttatttgta
    13921 ctgctgcgct gtgggaagca aacgagttat ttttcacaca aaaaaaccga gaacttgaat
    13981 aaacaagttt ctcggtttca tcattttcct tttatcggct tagtaccagc cgtttgctaa
    14041 ccagaatgtt ttagcagcat cccatgaacc ataacgagat gttacatatt gttctgctac
    14101 acgttcttga ttggcagctg aatagtcgcc gtttaagtat gaagcatcta attgataacg
    14161 gccgatgtaa cgtccgttag tagctgtgta tgaaccgcct gattcttttt gtgcgatcca
    14221 ttcttttgca gagcttgaag ttggcgctgt tgcagcagct ggggcagcag gagctacttc
    14281 aactgtttca acaggtgcaa cgtatgtatg ttcttgcgtt gctgtttcag ttgtagatgc
    14341 tgtattttct gttgttttgt tttctgttgt tgctgaagaa tcatttgaag ttgggattgt
    14401 taattgttca ccaacataaa tgcggttgat gtttgcgatc gaattttttt cagcaattgc
    14461 atcgatcatt gagttgtctc ctgcaaattt ttgtgagatc ttagataatg aatcacctga
    14521 ttttactgta taaacttcat cagcgtgagc atttgtagtt cctacaaaca ttgcagtagc
    14581 accagcagca aaagttgtac ctaaaacaat tttcttaaat gaattcatat taaactcctt
    14641 tttttctccg ttttttattc tttcgacgct attaact
//
LOCUS       DAZKNO010000060        14588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000060 DAZKNO010000000
VERSION     DAZKNO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.061"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(301..996)
                     /locus_tag="ACG8MY_001378"
     CDS             complement(301..996)
                     /locus_tag="ACG8MY_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0430574.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(1086..2186)
                     /gene="ychF"
                     /locus_tag="ACG8MY_001379"
     CDS             complement(1086..2186)
                     /gene="ychF"
                     /locus_tag="ACG8MY_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0430575.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2195..2386)
                     /locus_tag="ACG8MY_001380"
     CDS             complement(2195..2386)
                     /locus_tag="ACG8MY_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0430576.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2407..3297)
                     /locus_tag="ACG8MY_001381"
     CDS             complement(2407..3297)
                     /locus_tag="ACG8MY_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0430577.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3284..4051)
                     /locus_tag="ACG8MY_001382"
     CDS             complement(3284..4051)
                     /locus_tag="ACG8MY_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0430578.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(4063..4776)
                     /gene="rsmG"
                     /locus_tag="ACG8MY_001383"
     CDS             complement(4063..4776)
                     /gene="rsmG"
                     /locus_tag="ACG8MY_001383"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0430579.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(5008..6909)
                     /gene="mnmG"
                     /locus_tag="ACG8MY_001384"
     CDS             complement(5008..6909)
                     /gene="mnmG"
                     /locus_tag="ACG8MY_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0430580.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6926..8323)
                     /gene="mnmE"
                     /locus_tag="ACG8MY_001385"
     CDS             complement(6926..8323)
                     /gene="mnmE"
                     /locus_tag="ACG8MY_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0430581.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8622..9005
                     /locus_tag="ACG8MY_001386"
     CDS             8622..9005
                     /locus_tag="ACG8MY_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430582.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(9173..9931)
                     /gene="jag"
                     /locus_tag="ACG8MY_001387"
     CDS             complement(9173..9931)
                     /gene="jag"
                     /locus_tag="ACG8MY_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0430583.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9949..10767)
                     /locus_tag="ACG8MY_001388"
     CDS             complement(9949..10767)
                     /locus_tag="ACG8MY_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0430584.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10764..11120)
                     /gene="rnpA"
                     /locus_tag="ACG8MY_001389"
     CDS             complement(10764..11120)
                     /gene="rnpA"
                     /locus_tag="ACG8MY_001389"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0430585.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11238..11372)
                     /gene="rpmH"
                     /locus_tag="ACG8MY_001390"
     CDS             complement(11238..11372)
                     /gene="rpmH"
                     /locus_tag="ACG8MY_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0430586.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11895..13229
                     /gene="dnaA"
                     /locus_tag="ACG8MY_001391"
     CDS             11895..13229
                     /gene="dnaA"
                     /locus_tag="ACG8MY_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0430587.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13427..14557
                     /gene="dnaN"
                     /locus_tag="ACG8MY_001392"
     CDS             13427..14557
                     /gene="dnaN"
                     /locus_tag="ACG8MY_001392"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0430588.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         4041 a         3070 c         2622 g         4855 t
ORIGIN      
        1 tttgaattgg ttgggtttat taccgcagat gcaaactttt gaacaccgtt tagtggagag
       61 gctgtgaaaa agctgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca
      121 catttttgca taaattggac ttaataccga gaaactggct tttgaacacc gtttatcgta
      181 aaaagggact gtgacaagac ttttgtcaca gtcccttact attatttaac tatggataaa
      241 caaaatgagg ttctttacta cccctagata tctagcaaaa tagctgatct ttttgctagt
      301 ttatcttctt gggacggata aacttccaat aatcccgtat tttttcttca agtagtaacg
      361 gattgctaaa gcagttgccc cgataatgat aactactact ggatctaata ctggattgat
      421 aactggtggc aatagtgttg tcgcagaaaa cagtacgacc caaagaagtg tcgttccacc
      481 taggataagc aatgtcttac ccattcctgg tttcttgctt cgatctgctc cgggatattc
      541 atattgatag atgtatttgt acatcatata aaagacccag ccaccgattg cagaacctag
      601 aacgagtgta gaaaagccat aagctggcgt tgttccacgt gaaaacaatc cggtaatcgc
      661 aatcatgata cttaatagac caagcaacag caacgtatta tctaaccaca tcaataaagg
      721 ggtggatgaa ttttttactt ctgctggctt attgatgatt gcttccgttc tttcagagac
      781 tgtcccaaac aattgacggg ctgttcttcc gcctttttgt tctgcaacta aagtaggcag
      841 aatgtcatga agggctttta ctttttcttc ttcagacaaa ttcgctgctt ctaaagattt
      901 tttcaaatca aaaatgtact gttgatttct tttggttaat tttgtttcta aactattgtt
      961 ttcttttacg attgctcgta attgttctgg ttccattatc taatagtcct ccttagccgt
     1021 tccttttctc aacggtcgtc acaaaagaca ttctaccata ttctacagct agtttgatag
     1081 cccttttaca cattaaagcg gaataacatt acgtcgccgt cttgtacgac ataatctttt
     1141 ccttctaatc gaacacgtcc agcttctttt gctgcatgca tgtttccgta atgatccaag
     1201 tcttcaaaag agacagtttc tgcacggata aatccgcgtt cgaaatctga atggatgatt
     1261 cctgctgctt gtggtgcttt gattcctttt ttgaatgtcc aagcacgtac ttcttgttcc
     1321 ccagcagtaa agtaagtagc taagcctaac aaatcataag cagcacggat caactgatcc
     1381 aaacctgatt cttcgattcc taaagcttct aaaaattccg ctttgtcttc ttcatctaat
     1441 tctgcgattt cttcttctgc acgagcactg accacgatca cttctgcatt ttcttctgca
     1501 gcaaagttgc gcacttcttg tacataatga ttcgcgtctg catctgctac gtcatcttct
     1561 gagacattgg ctacataaag aattggtttt gtcgtcaata aaaatagtga tttaacgatt
     1621 ttttgttctt cttcagtaaa ttcgatcgtt cgggcagatt ttccttcttc cagtacaggt
     1681 ttgattttat ccagtacagc tagttctgcc actgcatctt tatctttggt tttagcaact
     1741 tttgctacac gagtatagcg tttgttgata gattctaagt ctgctaacac tagttccaag
     1801 ttgatcgtat caatatcttc tagtggatca acacgtcctt ctacgtgagt aatattatca
     1861 tcatcaaaac agcgaacaac gtgacaaata gcgtctactt ggcggatatg gcttaagaat
     1921 tgatttccca aaccttctcc tttacttgcg ccttttacga ttccagcaat atctgtaaat
     1981 tcaaaagtcg tcggaacagt ttttttcgga tgtacaagtt ctgttaagcg ttgcaaacgc
     2041 cagtcaggta cttctaccat ccctacgttg gggtcgatcg tcgcaaaggg atagtttgct
     2101 gcttctgctc ctgcttttgt gatcgcatta aacaaagttg attttcctac gttgggcaac
     2161 ccaacaattc cagcagtcaa tgccataaat ttattcactc tttctctgca tttttttcaa
     2221 ttactttttt taatttcttt tcaaaatctc gacgggtcat catgacgata tgtccgcaat
     2281 tcatacatct gattttgata tctgcgccca ttcgaatgac ttcccagcga ttcgtctggc
     2341 aagcgtgagg ttttttcatt tcaacgatat cgtgcaagtc gtacataaaa atagcctcct
     2401 ttttgtttat tcttcatcaa attgaatatt caagatatca agaattcgag tcaaatcttt
     2461 ttgagaaaga tattcaatct cgatcttacc tttcccattt ttttcttgga tagctacact
     2521 tgtaccaaat ttatccatca aacgatcttc gctttctcgg atataatatg gtttttcctt
     2581 tgctagcctt gggatttttt tcttttcttt tccatcattt tgatttaact cagaaacaag
     2641 ctgttccagt tggcggacag ttaaattttc agttactgca cgattggcta atttcaagat
     2701 caatgtttta ttttttaaac ctaacagcgt acgggcttgt cccatagata aacgctgatc
     2761 ttgtaccatt tccttgacaa gatccggtaa agaaagcaag cgcaaataat tggcgatata
     2821 aggtctgctc tttcctaaac gactagctac ttctgcttgt gttagtttca ggtttttcat
     2881 cagcatctca tatgcttctg cttcttctaa cggattcaga tcttctcgct gcaagttttc
     2941 cagcactgct acttgcatca tcgcttcttc gtcaaagtct ctaacgatag cagggatcgt
     3001 ttctttttca gctagtttgg acgctcgaaa acgtcgctcc cctgcaataa tctcatatcc
     3061 ttttactgcg gaacggcgta cgatgatggg ttgaaagaca cctgattgtt caatagaatt
     3121 agctagttcc tgcaaagata cttcttcaaa tgttttacgg ggctgatacg gatttggtcg
     3181 cagctcattc aatggtagct ggacaacggt atcatctttt acatccaccg tctctaagtt
     3241 ttctaaatct tgaaacagtg catcgattcc tcggcctaat cctttacttt tcttcacgac
     3301 ttaccacttc ctttgccaat gcttggtaga cttcagctcc tcgagaacgt ggatcgtagt
     3361 cgatgatcgg ttttccatga ctaggtgctt ctgataaacg aatatttctt ggaatgatcg
     3421 tttcgtaaac tttttcgcgg aagtatttac gtacttcttc tacgacttca tttcccagat
     3481 ttgttcgtgc atcatacatt gtcagtaaga ctccctcgat ctctaattct ggattaaagt
     3541 gtttttggac taaacgaacc gtattcagta attggcttaa tccttccaat gcgtaatact
     3601 cacactgaac aggtatcaaa atcgaatcac ttgctgtaaa tgaattgatt gtcagatggc
     3661 caagagaagg cgggcaatcg atcaaaatat aatcatactg actgcttact tctgctaaag
     3721 atccttttaa tcttgattct cttgccatca ttgacgtcaa ttcgatctct gctcctgcta
     3781 attggagagt cgcaggcaca atactcaaat tttcgtgttc agtgatcaaa gttgcctcat
     3841 caattggcaa ttcattgact agaacatcat agatgtctct tgtcacatca ggttttctga
     3901 tcccgacacc actggtagca tttccttgtg catccatatc cactagcaat acttttttgc
     3961 caaggctagc aagacaagca cctagattca ccgtcgtcgt tgttttgccg acgccacctt
     4021 tttggttcgc aacagatatt atttgtgcca tctcttctcc tcctatttga ttggctgctt
     4081 attaggcagt ccgggctttc ttgggtattt ctttggggtt ggtttctttt tttgaataac
     4141 gatgatatgc cgttgatcag agacattcgg caaggtaacc gcctcttctt tgaggaattt
     4201 accacctaat aaagcaatcg cttgttttgc ttctgtcaat tcctcttcac tttttgctgc
     4261 tttcaatgca taaaaatagc cttcttgttt agccaagggc aaacacaatt ccgctaacac
     4321 attcaaacga gcaaccgcac gagcagtgac aaggtcaaat gatgcacgaa agtgaggatt
     4381 ttgcccaaat gtttctgccc ggtcatgata taaggaagca tcgattccta ataatcgggt
     4441 taactcagat aagaaggtta ttcgtttatt cagagaatca acgatcgtta tctgcagttc
     4501 tggaaaggca atttttaaag gtatgctagg aaaccctgcc cccgaaccaa catcgcataa
     4561 agataaaggt tgcgtaaagt ctacttcaaa tgctaatgta agtgaatcgt aaaaatgctt
     4621 cagatatacg tctttttcat ccgtaatagc tgtaaggttc atcttttcgt tccattcgac
     4681 taatgtccga taataaatag cgaattgatt catttgatga tcggtaagct ctatcccttt
     4741 ttctgccaag gattggcgaa attcttctgg tatcataaat attcctttca ttgtgaaaca
     4801 gcaattgttt cacagattct ctccctactt taacgcaaaa tctctagaca tgcaatcctt
     4861 ccatatggat tcttaaaaat ttgaaaagta agaaaagccg atagactttt tctttctagt
     4921 atgactcata aaatatacac agaagagaga actagctttg agcaatttct gcaaaaacta
     4981 gttctctctt ctatttatta ttacgtatta cgctggttca ctgactttcg caatcttccc
     5041 ttgttcgata tagaccatta ggatacttag gtctgcaggg tttacgccac tgattcggct
     5101 agcttgggca attgtttctg gttggatttt ctgcaatttt tgttttgctt ctgttgctaa
     5161 tccgttgatt gctgcgtagt cgatgttttc tggaatcttt ttcgcttcca tgcgttttaa
     5221 tttttctact ttttccatgg cttttttgat gtagccttca tatttgattt ggatttctac
     5281 ttgttcgatt tcttttcgtg tcaattcttc attttctgga ataaaccgta gaagttctgc
     5341 ataagtaatc tcaggacgtc ttaaaaagtc gcttgctagt acaccatctt ttaatggtgc
     5401 cgcattaatc gttgctaaaa agtcttggac ttcttctgtt ggtttgattc gtacattcat
     5461 caaacgtttg atttcttgtt caactgcttc ttttttcttc aaataattct tgtattgatc
     5521 ttcttttact aagcctacac ggtgacccat ttctgttaat cgcaaatctg cattatcatg
     5581 acgtaaaagc agacggtatt cagcacgtga agtcagcaaa cgataaggtt cgttcgttcc
     5641 ttttgttacc aaatcatcga tcatgacacc gatgtaaccg tcacttcgtt tgatcactaa
     5701 aggttctttt ccctgaactt tcaaagctgc attgataccg gcaatcagcc cttgtccagc
     5761 cgcttcttca taaccgcttg taccatttgt ttgtccagct gtataaagat tttcaacgac
     5821 tttagtttct aatgtcggac ggagctggtg tggctctact acgtcatatt cgatagcata
     5881 tcctgtacgc atcatttcta ctttttctaa tccagcaata gaatgaagca tgttgacttg
     5941 aacatcctct ggtaaagaag tagataaacc ttgaacatag acttcttccg tatttaagcc
     6001 ttctggttcc aaaaataatt gatgacgtgg tttgtctgca aaacgcacga tcttatcttc
     6061 gatggatgga caatagcgag cgcctacccc ttcaacgatc cctgtaaaca ttggggcacg
     6121 atgtaagttg tctcgaataa tttggtgagt cgtctcacct gtataggtca gccagcaaga
     6181 ttcctgatct agtttatagg cactatctgg tgtgctgaag ctaaaatgat tcggctcttt
     6241 gtcaccaggc tgctcttctg taacagaata gtcgatcgta cttgatttta ctcgaggcgg
     6301 tgttcctgtt ttgaaccgtt cgatagcaaa gccaagttct tttagatgat tcgctagtcc
     6361 aatagaaggt tgagagttat tcggacctga tgaatatttc aattcaccga taatgatttc
     6421 tccgcgaagt gctgttccgg ctgtaataac aactgctttg gcccggtagc tcgctcctgt
     6481 ggacgtcact actccatgac aaacaccatc ttcgacgatc agttcttcga cgattccttg
     6541 tcttagcgtc aaatttggtt ctttttcgat cgtatgtttc atttctgttg aataagcatg
     6601 tttgtctgct tgtgctcgca atgctcggac agctgggcct tttcctgtgt tcaacatacg
     6661 catttggata taggttttat cgatattctt acccatttct ccgcctaacg catcgatttc
     6721 tcgaacgaca acgcctttag ctggtccgcc tacagaagga ttacatggca tgaatgctac
     6781 catgtccaaa tttattgtca gtaataatgt ttttgatccc atacgtgcag cagcaagtgc
     6841 agcttctgaa ccggcgtgtc ctgcgccaac aacgatcacg tcatacgcat ctgcttcata
     6901 atgttccaat cgtttgttcc tccttttatt ttcctaaaca aaattgactg aataattgag
     6961 tgattagctc atcttgtaca ctgtcgccga caacttctcc tagatagtcc cagcaacgcg
     7021 tcatatctat ttgaacgaga tccacaggca ttcctgaatc aatacctgca atgacttctt
     7081 caagtgagag ggaagctttt tctaacaacg cgatatggcg ggtattagaa acataagtag
     7141 cgtctctttc tcctgtttct ccgctgaaga ataaatcaga aatagctgat tccaaacgat
     7201 ctagtccgtc gtttttagct acggaaattg aaaaaactgg ttcattttcg atcaatttct
     7261 tcaatttttc ttgttccagc tgtgctggca aatctgtttt atttagaaga atgatccgtt
     7321 tcaatccact tgtcgcttcc aataattgtt catcttctgc tgtcagtggt tcgctttggt
     7381 ttagaacaag taagatcaag tctgcttctg ctaatgcttt tcggcttctt tcgaccccga
     7441 tccgttccac cacatcttct gtttcccgaa ttccagctgt atcgattagc ttgagcggta
     7501 ccccccgaac attgacatac tcttcgatta catctcgagt cgttcctgca atatccgtaa
     7561 cgattgcttt ttcttctctt aacaaatggt ttaacaaact tgatttgccg acatttggcc
     7621 ggccgatgat tgccgtactt aacccttccc gcagaacctt cccttgcttg gatgtagcaa
     7681 gtagcgcttg gattctttgt tggatcatct gcgctttttc aagcaataac ttcgtagtca
     7741 attcttcgac atcatcatat tctggataat caatatttac ttctacttgt gctaatgtct
     7801 ctaaaatctc ttgtctcaat gagcggatca aagcagacag attgccgtct aactgattaa
     7861 gagctagtcc cattgctttg tctgtttttg cccgaatcaa atccatcaca gcttctgcct
     7921 gtgaaaggtc aacacgacca ttcaagaatg ctcgttttgt gaattcgcct ggctcagcta
     7981 atcttgcacc ttctcttaac aatagctgca aaatttgatt gactacaacg atcccaccgt
     8041 gacaattgat ttctactaca tcttcccgcg taaatgtctt tggtgctctc atcacggaga
     8101 ccatgacttc atcgacaagc tgattgctct tgggatcaac gatatgtcca taatggatcg
     8161 tatgacttgg tacttctgat aatcgtttgt ttccagattg ataaacttta tctgctagtt
     8221 gcacagcttg atcgccactt aaacgaacga tactgattgc accttctcca ggtggggtag
     8281 aaattgccgc aatcgtatca aattctaaag taatttcagc cattttttca cttccttttt
     8341 cattttgcgc acaaaaaaag tgcccactcc accctttatg cacaataaac ggtgaacttc
     8401 gcactttgac tgcttgttct tgattaaatt ctggtcatag attatcacac ttgctataag
     8461 gaatcaagtg tttaaaacaa ttagatgaag ttgagcctgt tctgcttcca ttaccttata
     8521 tttaattgtc cttggatgtc tctttgcgtt ctttggttta ttcgaagaga agatactgta
     8581 taaacaaggc tttatctttc ttaaaaaaaa cggtacacta aatggtagcg ctatctaaac
     8641 tatacacttt tgaaaggaga aaatatgtga gcccgttcag atctgctgca ttggaagaag
     8701 aaagtgaccc tcggatcaac gatgtcgttt tatctacagg gaatatcaac cagaaatata
     8761 tcgtccgcga tatcgttttt gttgtggaat gcttagaagc tgatttgttt gacccatctt
     8821 ttcccaaaga tgaccttttc ttaacaacga agaggaaatt aaagacgaag gtacttgaat
     8881 atggtgcgaa tgcagtaatc aactgtcatt ttgagtatca gcaaaaaaaa cgcgacggaa
     8941 agtattttct ggaacttttt gcttatggaa cagtgatcca atttatccaa acaacgattg
     9001 gttaaatgaa acagacaaaa aaagcggatc aaaactgaag acagtttcga tccgcttttt
     9061 tctatcaaag ttagtgacta cttcatcgta tactctgata agaatgattt ctagtaaaat
     9121 atacttggtt tgaaaaactt tgtcaacgtg atttattcaa aaagtaatgg aatcaataat
     9181 attttttagc aggttctact actaaataac gataaggttc ctcaccttcc gaatgtgttt
     9241 ttacatgttc atctttgctt aatgcagcat ggatttgttt acgttcaaat gcaggcattg
     9301 gttctaagaa aacaggctgc cctgtccgtt ttactttttc agccgtgcga tcagctaaac
     9361 gctgaatgat ctcttggcgc ttttcacggt aattgcctac attgactact acagacagct
     9421 tatttcttgc taaacgatgg atgaagactt gtgccagata ttgcaatgca ttcagtgttt
     9481 ttccatgttt cccaatcagt atgccttgct tttgtgtttc taaatggaaa acaaccatat
     9541 tatttgattc tctttccatt ttgacaagag ctggagcatc aagctgtttt gaaatttcag
     9601 taagataaac tgccaattcc gttaaagctg cttcatcacc tagctcactt acttcttctg
     9661 ttttttcttc aagaatcgtt tcgttgacta cctcgatctc agctgttggt tcttcttcca
     9721 aaatttcttc tacagtctgt tctactgttt cagaaacttt ttcgctgata cttggctgga
     9781 tcgaaagccg tgcattcttt cgacccattc ctaggaaacc tttttttcct tcttccagta
     9841 tttcgatttc gacttggtct ttggccaaac ccaaatcagc cagacctagt tggacagctt
     9901 cgtctattgt tgcagcttca taaatcggca ttagcattac ctcccctatt atttccgttt
     9961 tttcttagga ttcattgctt tcttaagagc acgttctctt tcgcggattc tccgtgcttc
    10021 ttcttcacgc tcttttcgaa ttttgaaagg gttgttgatc agcagcgttt gtacaacttg
    10081 gaaggcattg gaaactaccc agtataatga aagaccgctg gctaaattga cacccatcac
    10141 taaaatcatt acaggcatca cgaaattcat gatcttcatt gatgcattcg attcaatttg
    10201 actcatgcta gataaatacg tacttgcaaa agtaaagatt gctgctaaaa tcggcaaaat
    10261 aaatgttgga tccgcgttcc ctaagttcaa ccataagaaa tgcccttgtt tcaactctgg
    10321 tacacgtgaa atagattgcc ataacgccat caagattggc atttgtacta ataaaggaag
    10381 acaaccagca tatggattga cgccattttc tgcatataaa cgctgctgct cttctcttaa
    10441 cttactttgt gtttccggat ctcttgaact atattgttgc tgcagtgctt ttaatttcgg
    10501 ttgtaattct tgcgttttac gcatgctctt tgtttggaaa tgcatcaacg gcaataggat
    10561 cacgcggatg atcagtgtaa ataaaatgat ccctatccct gcattaccaa atgacaacgc
    10621 cttgatcgcc tcagcaaagt aataaacaat ataacgatcc caaattcctg tactctgtgc
    10681 gctgacttcc ccagttccgc aaccggataa gacgatgacc aaactaacga taccggccat
    10741 caaaagtaaa cgtttgtatt tcttcactta ctcttttcct ctctgtcgat tatttttgca
    10801 agttttaaga cgtggactaa attggagcga acctcatcat aagtcaaccc cttcacactt
    10861 ggtctagcaa tcacaataaa atccaactcc ggatcaattt cttcttttat agaataaact
    10921 gctgctcgga tttttcgttt gacttcattg cggtttaccg cgttgccgac cttttttcct
    10981 acagataaac caactcgaaa atgaggctgt cccggttttt ccaaacggta aacgacgaat
    11041 ttgcggttag caaaagaatc tcccgtatgg aacacggctt gaaactctcg ttcttttttt
    11101 actcgatacg cttttctcat tagttttctt tccttttcta tctctcactt acgctcataa
    11161 tcataccata attacgattg tcctttcatc atttccctaa aaaagagtat aaaaaaaacc
    11221 actgaaaccc cagtggccta agctgaaaga acttttcttc ctttgcgacg acggctagct
    11281 aacacacgac gtccattttt agtactcata cgtttacgga atccgtgaac tttttgacgt
    11341 ttacgtttat ttggttggta tgttcttttc atatattcca cctccaaaat gttcttatct
    11401 attttataaa cacagacata ctttgatagt ataacgacaa agtagtgatt ttgtcaatat
    11461 aaaatagaag atcgattgtt aaaacttatc cacaagatta tttttttcta tgggaatcct
    11521 atgtggaaaa agttatgcac agggattatc cacagataat aaaaggctgg ttttacacag
    11581 aaaacacacg tgtaaaaaaa gtaatccaca atcaggcgat ttcctgtgta ttccgtctcc
    11641 taactattgg tatatctgta ttttttatcc acagacgcaa gaggaaatct attggttttt
    11701 acgagttttc cacctgtgca ttagtggtgt ggaaaacttt ttttgacaac tgtgtttttc
    11761 tcttttttct ttttgtcgat tgtgtggata attgtaatta aaaaattttt ttcttcggtt
    11821 tctgtggaaa actattgaaa aaaatgctaa attagtacta gttattcact ttttaatgaa
    11881 ggaggaggcc ttatatggta tccctcgatg ctttatggaa tgaattaaaa gcaacatacc
    11941 aaaaggattt atcgcctgct agctataata catggatcga aacagcgaac cctcgaaccc
    12001 ttgatcaaaa ccagctcgtt gttgaggttc ctagcaagat tcataaagaa tattgggaaa
    12061 aaaacctggc gacaaaaatc gttgaagtcg gttatatgtt atcaggaaat gagattatcc
    12121 ctcgctttat tactggcgaa gaagcagagc aagaagaagt tatagaagaa aaaaatccaa
    12181 aagttgtggc acctagtccg ctaaaaaaag ccatgctgaa ccctaaatat acctttgata
    12241 cgtttgtcat tggtaaagga aaccagatgg ctcatgcagc agcgctcgtt gtagcagaag
    12301 atccaggttc tatttataac ccgctcttct tttatggagg cgtgggactt ggtaagactc
    12361 acttgatgca tgcaattggt catcaaatgc tgcaaagtca gcctaatgcc aaagtaaaat
    12421 atgtaagcag tgaaactttt gctaatgatt tcatcaattc gatccaaaac aaaacagcag
    12481 aagagttccg tcaagaatat cgaaatgttg atttactgct agtggatgac attcaatttt
    12541 ttgcagaaaa agaagctacg caagaagagt ttttccatac tttcaatgcc ttgtacaatg
    12601 aagggaaaca aatcgtacta accagtgatc ggttgccaaa cgagattccg aaactgcaag
    12661 aacgccttgt ttctcgcttt gcatgggggc tgtctgttga tattacaccg ccggatcttg
    12721 aaacaagaac agcgatttta cgtaaaaaag cggcggctga acgtctagag atcccagatg
    12781 acacactaag ctacatcgca ggacaaattg attctaacat tcgtgaatta gaaggtgcgc
    12841 ttgttcgagt gcaagctttt gctgcgatga atagtgaaga tatttcgact agtctagctg
    12901 cagacgcact gaaaacgtta aaatcaggaa aaggacatcc tcaattgtca atcctgcaga
    12961 tccaagaaga agtggcaaag tattatcaca tccaattgaa agatctcaaa ggaaagaaac
    13021 gcgtcaaatc tattgttgtt cctagacaaa tcgctatgta tttggcacgt gagctcacag
    13081 ataattcttt accgaaaatc ggagctgaat tcggtggcaa ggatcataca acggttatcc
    13141 atgctcatga aaaaatccag cagctgatgg attctagtgt cagtatgcaa aatgaagtat
    13201 cagaaatcaa aaacttttta ctaaattaat ttgtcgattc tatcaactga aaatagcatg
    13261 tggataaaag aaaaaaacgt tcaaaagtta tacacacttt ttccacagct gttttttcgt
    13321 ttctctctta cgttttttga gttttccaca gaatcaacat gccctattac ttttattatt
    13381 ctttttaact aataataaat aaacaaacat tcaaaggaga tacatcatga aagttacttt
    13441 aaaccgagct agctttatgc aggaattgca aactgttcaa cgagctattt caagcaaaac
    13501 cacgatccct attttgacag gtgtaaaaat cacactgaca caagaaggtt tgactttgac
    13561 ggggagcaac gctgatatat caattgaaac ttttttgtct gttgaaaacg aaaaagcaaa
    13621 tatgcaaatc gaatctactg gttccattgt tttacaagca cgtttcttta gcgaaatcat
    13681 tcggagactt cctgaagaaa catttacttt agaagtttta gaaaataaac aagtagcgat
    13741 cacttctgga aaagcgaatt ttatcgtaaa tggattagat gcagataact atcctcatct
    13801 tcctgttgtc gaaagccata accagatgaa attacctgta cacgtattga ctaaactaat
    13861 caacgaaaca gtttttgctg tctcacaaca tgagagtcgt ccaatcttga caggtgtgca
    13921 tttcatttta tctgataatt ctttattagc tgtagctact gattctcacc gtctaagtca
    13981 acgcgtgatt ccagtagaac aagcggctga tcattttgat attgttattc ctggaaaaag
    14041 tttgatcgaa ttatctcgct cattaacaaa tgaagaagaa atcgtcgaaa tcagcattat
    14101 ggaaaaccaa gtgttgttca aaacagaaac gatgtatttc tattcccgtt tgctagaagg
    14161 aaactatcca gataccaatc gtttgattcc ttcaagtttt aatacagagg ttgaattttc
    14221 tgttcctagc tttttagcgg cgatcgaacg ggcttcttta ctttctcatg aaggccgtaa
    14281 caacatcgtt cgtttatcta ttcgtccaga tgcggttgtt ttatacggaa attcgccaga
    14341 aatcgggaaa gtagaagaaa gcttgagtta tacagcaagc agtggcgatc cattagatat
    14401 ttctttcaat ccggattata tgaaagcagc gttgcgtgca tttggagata tgagcatcaa
    14461 agtgaaattt atctctgcta ttcgtccatt tacattggaa ccaacggaag atggcgttca
    14521 atttattcag ctgattactc ctgtacgtac aaattaacaa tgattttcaa tgttcttgaa
    14581 aatttaat
//
LOCUS       DAZKNO010000061        14554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000061 DAZKNO010000000
VERSION     DAZKNO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.062"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..1854
                     /gene="yfcC"
                     /locus_tag="ACG8MY_001393"
     CDS             115..1854
                     /gene="yfcC"
                     /locus_tag="ACG8MY_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0430589.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2011..3195
                     /gene="iadA"
                     /locus_tag="ACG8MY_001394"
     CDS             2011..3195
                     /gene="iadA"
                     /locus_tag="ACG8MY_001394"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0430590.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3306..3509
                     /locus_tag="ACG8MY_001395"
     CDS             3306..3509
                     /locus_tag="ACG8MY_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0430591.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3610..4272)
                     /locus_tag="ACG8MY_001396"
     CDS             complement(3610..4272)
                     /locus_tag="ACG8MY_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430592.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4285..5787)
                     /locus_tag="ACG8MY_001397"
     CDS             complement(4285..5787)
                     /locus_tag="ACG8MY_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0430593.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6335..8014
                     /locus_tag="ACG8MY_001398"
     CDS             6335..8014
                     /locus_tag="ACG8MY_001398"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0430594.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8141..8620
                     /locus_tag="ACG8MY_001399"
     CDS             8141..8620
                     /locus_tag="ACG8MY_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0430595.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8839..9519
                     /locus_tag="ACG8MY_001400"
     CDS             8839..9519
                     /locus_tag="ACG8MY_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0430596.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9527..10858
                     /locus_tag="ACG8MY_001401"
     CDS             9527..10858
                     /locus_tag="ACG8MY_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0430597.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10873..11835
                     /locus_tag="ACG8MY_001402"
     CDS             10873..11835
                     /locus_tag="ACG8MY_001402"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0430598.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11946..12284
                     /locus_tag="ACG8MY_001403"
     CDS             11946..12284
                     /locus_tag="ACG8MY_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0430599.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12363..13070
                     /locus_tag="ACG8MY_001404"
     CDS             12363..13070
                     /locus_tag="ACG8MY_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0430600.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13173..>14554)
                     /locus_tag="ACG8MY_001405"
     CDS             complement(13173..>14554)
                     /locus_tag="ACG8MY_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430601.1"
                     /translation="KRTIKELIRKINVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKL
                     AVFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSL
                     KKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQ
                     IEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVS
                     RPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQ
                     FPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLRE
                     KIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQL
                     FRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKK
                     GYENKTKRM"
BASE COUNT         4622 a         2515 c         3136 g         4281 t
ORIGIN      
        1 aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg
       61 attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag
      121 aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg
      181 tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca
      241 ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt
      301 actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt
      361 gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa
      421 aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat
      481 gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact
      541 tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt
      601 ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg
      661 atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg
      721 atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta
      781 ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta
      841 attccattct caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta
      901 gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg
      961 tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc
     1021 cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca
     1081 gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt
     1141 tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc
     1201 ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat
     1261 tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct
     1321 gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt
     1381 gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc
     1441 ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg
     1501 acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt
     1561 ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg
     1621 gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt
     1681 ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt
     1741 gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg
     1801 tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg
     1861 agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt
     1921 acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat
     1981 cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt
     2041 gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta
     2101 gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga
     2161 aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa
     2221 ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta
     2281 acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc
     2341 agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac
     2401 cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa
     2461 attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa
     2521 gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg
     2581 atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta
     2641 gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa
     2701 ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc
     2761 gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa
     2821 cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga
     2881 agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag
     2941 gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc
     3001 ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc
     3061 gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca
     3121 gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc
     3181 gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg
     3241 tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa
     3301 gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag
     3361 gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac
     3421 aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca
     3481 agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa
     3541 taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta
     3601 tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc
     3661 acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg
     3721 ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt
     3781 aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg
     3841 ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc
     3901 tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat
     3961 ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg
     4021 gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg
     4081 atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa
     4141 agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt
     4201 cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc
     4261 tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa
     4321 tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa
     4381 tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc
     4441 aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt
     4501 acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg
     4561 gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc
     4621 taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct
     4681 ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat
     4741 aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga
     4801 aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat catttgattg
     4861 taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt
     4921 atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt
     4981 tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa
     5041 tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc
     5101 ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc
     5161 atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc
     5221 tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt
     5281 tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc
     5341 tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact
     5401 agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact
     5461 ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga
     5521 aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg
     5581 ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg
     5641 tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat
     5701 aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact
     5761 tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata
     5821 atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga
     5881 ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa
     5941 gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc
     6001 tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt
     6061 tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg
     6121 tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg
     6181 tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga
     6241 ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct
     6301 ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt
     6361 aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt
     6421 tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat
     6481 ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca
     6541 tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt
     6601 attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc
     6661 caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac
     6721 agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa
     6781 aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc
     6841 aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt
     6901 actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc
     6961 agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc
     7021 aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt
     7081 tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact
     7141 tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga
     7201 agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat
     7261 ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac
     7321 tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc
     7381 tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa
     7441 tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga
     7501 gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa
     7561 tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc
     7621 aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa
     7681 cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac
     7741 tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt
     7801 ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt
     7861 tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga
     7921 tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat
     7981 tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct
     8041 atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc
     8101 ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt
     8161 cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt
     8221 agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa
     8281 aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga
     8341 tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg
     8401 aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat
     8461 aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga
     8521 agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga
     8581 aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa
     8641 gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt
     8701 atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag
     8761 ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga
     8821 aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa
     8881 atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac
     8941 ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac
     9001 ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag
     9061 attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa
     9121 catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa
     9181 gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga
     9241 aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa
     9301 gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa
     9361 gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat
     9421 actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa
     9481 ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca
     9541 tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag
     9601 catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga
     9661 aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt
     9721 aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt
     9781 tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac
     9841 taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc
     9901 ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa
     9961 tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc
    10021 ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa
    10081 aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca
    10141 gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt
    10201 tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg
    10261 gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa
    10321 aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc
    10381 ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc
    10441 gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg
    10501 aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa
    10561 attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag
    10621 cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt
    10681 gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat
    10741 tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat
    10801 tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa
    10861 agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa
    10921 atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta
    10981 gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt
    11041 gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg
    11101 ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg
    11161 gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg
    11221 tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt
    11281 cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga
    11341 gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata
    11401 ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg
    11461 aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca
    11521 ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca
    11581 gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg gatttttgtt
    11641 cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa
    11701 ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat
    11761 ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc
    11821 agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt
    11881 atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa
    11941 agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag
    12001 gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca
    12061 tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg
    12121 tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa
    12181 aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat
    12241 ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct
    12301 tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga
    12361 aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg
    12421 gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc
    12481 aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc
    12541 aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt
    12601 caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga
    12661 taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat
    12721 tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct
    12781 tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg
    12841 ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc
    12901 ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg
    12961 caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc
    13021 tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag
    13081 ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt
    13141 atttggaata agaggatgtg acaagaattt tgtcacatcc tctttgtttt gttctcataa
    13201 ccttttttca tatacagttc atgaaccgct tttattaact tgccaaataa cactgtattg
    13261 tgcgttgtat ccagatagaa agtctccgtt tcatctgaaa ttttctcatg ctgatctgta
    13321 atgatcaaat cagcatgttc tgaatatgcg gtaattttaa cagcatcttt gcgaaataac
    13381 tgctgcaaat ggctttgaag atgataattt cctgtaaact gtttggttat ctgcaaatgg
    13441 atgagtaact gctctttcgt ttctgtttgt agtaagtgtt ggatcaattg actaaggtat
    13501 tgactgattt ggtttctttc cccatttttt gaaaaaattg tatcgatttt ttctcttaat
    13561 ttcttttcct gtataaaaga actagaatga ttggacgtgg cttctggaaa gcaaagtgta
    13621 gaaaaactac tgctggattc acccaacagt tcgaaaaaaa caactgccaa cacaagaaaa
    13681 taaaaataaa ttggtatttt attttctaaa tggttgagag gaaactgttg agttaagtaa
    13741 gtcatcaatt gaccgacttg tcgacaaata aggtttttgc ttgtggaaaa taaaaaacca
    13801 atctcctgtt tttgtcgaaa agagatttga tttggaaaaa aacaatagaa aaaataatta
    13861 aagtaggctc tctctgtttc tgtctcttca caagggcgac tcactgaaaa agaagtaaag
    13921 tattctgaat aatcatgtgt atgctgcaat aagtccatca attcagtgat ttcagaagaa
    13981 gtttttggaa aatatgcctg ttgtttgttt cgtaatatga ataaagttaa cagcaagtag
    14041 atattttttt cttgttttga aattttttct atttgctcat ctgtactgtt catttttagt
    14101 gcatttaatt caagtgagga aaatggccaa tcgttacctc caaatgtatc tgaaagaaaa
    14161 aagtaaagat agagacggat agataactca tttccttcaa gagtacagca attttttaca
    14221 gataggatcc gtaaatggaa tttttttaag gaatgattga gctgttttgt cagtttttcg
    14281 agataggagg gcgatatata aagttgttca agtaaatatt tttttggtac agcagtttgt
    14341 ggataattga ttagtaacat caaatagtta aaacgattgg attcttttag gtagcataat
    14401 tttaacttag aaaaaacagc taacttttta gtttgatcgg tttctttggt ttggatctct
    14461 cctttatgat tagaataaat aaattcatca atcaataaaa attgcttgag tgatacattt
    14521 atttttcgga tcagttcttt gattgtccgt tttg
//
LOCUS       DAZKNO010000062        14226 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000062 DAZKNO010000000
VERSION     DAZKNO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.063"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..436
                     /locus_tag="ACG8MY_001406"
     CDS             <1..436
                     /locus_tag="ACG8MY_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430602.1"
                     /translation="SPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILS
                     DKINVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIED
                     YIWKLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(517..1086)
                     /gene="thiT"
                     /locus_tag="ACG8MY_001407"
     CDS             complement(517..1086)
                     /gene="thiT"
                     /locus_tag="ACG8MY_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0430603.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(1152..1254)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1260..2231)
                     /locus_tag="ACG8MY_001408"
     CDS             complement(1260..2231)
                     /locus_tag="ACG8MY_001408"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0430604.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(2325..3698)
                     /gene="glmU"
                     /locus_tag="ACG8MY_001409"
     CDS             complement(2325..3698)
                     /gene="glmU"
                     /locus_tag="ACG8MY_001409"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0430605.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(4201..5019)
                     /gene="purR"
                     /locus_tag="ACG8MY_001410"
     CDS             complement(4201..5019)
                     /gene="purR"
                     /locus_tag="ACG8MY_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0430606.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(5186..6010)
                     /locus_tag="ACG8MY_001411"
     CDS             complement(5186..6010)
                     /locus_tag="ACG8MY_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0430607.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5983..6663)
                     /locus_tag="ACG8MY_001412"
     CDS             complement(5983..6663)
                     /locus_tag="ACG8MY_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430608.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6686..7624)
                     /locus_tag="ACG8MY_001413"
     CDS             complement(6686..7624)
                     /locus_tag="ACG8MY_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0430609.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7869..8720)
                     /gene="ispE"
                     /locus_tag="ACG8MY_001414"
     CDS             complement(7869..8720)
                     /gene="ispE"
                     /locus_tag="ACG8MY_001414"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0430610.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(8874..11447)
                     /gene="mprF"
                     /locus_tag="ACG8MY_001415"
     CDS             complement(8874..11447)
                     /gene="mprF"
                     /locus_tag="ACG8MY_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0430611.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11588..12394
                     /gene="proC"
                     /locus_tag="ACG8MY_001416"
     CDS             11588..12394
                     /gene="proC"
                     /locus_tag="ACG8MY_001416"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0430612.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12473..14029)
                     /gene="rny"
                     /locus_tag="ACG8MY_001417"
     CDS             complement(12473..14029)
                     /gene="rny"
                     /locus_tag="ACG8MY_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0430613.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         4128 a         3113 c         2463 g         4522 t
ORIGIN      
        1 ttcgccgttt ttttataaac tacagaataa ttttgtatac ttcgatctta atgatcagct
       61 gaatcggatt tattatcgct atttagatga attttatagg atttttgcta gtaaattaag
      121 ttcgatcttg tcagataaga tcaatgtaaa aaaatctttt ttcaaaaaag aaacacctgt
      181 tgaaaaaatc agtgtggaat tattgaaaga aatgcgtaat ttgatgcagg aagggatcat
      241 tttttctttt gatcaagttg gtttgatcga tggtaaatat cgtatcccaa tctatgtgat
      301 tgaagattat atttggaaat taaaaggcac ccggtatcga gaagaggaaa tcatcgctac
      361 tttaatctac tctgtcaccg atcgacactg gcaattggta gaagatgccg atctttctta
      421 tctgccattg aagtagatct atttcttatc aaaaaataaa aaggaaacaa gaaactagga
      481 catgattctg tctcaagttt cttgcttctt ttttatttac agctcctgtc gtttatctgg
      541 cacaaaaatc gaaggagtcg tttgttggat ccctaataaa acgatgatcg ttacaatagc
      601 tgtactcaac ccgctggcac cattcattac gaaagaaaag agccaaggat tcatccccca
      661 tagtgcatac gctccccaga aaaccactcc tgcaataaaa tgccaaaaat atctggaaag
      721 tgcgccaatc aatgcaccaa acacgatgct ttttctaatt tgtttcgtat tatcgtctac
      781 atagcctttt tggacatttg aagccatgag ccctgctgcg cctgcaaaag tataggcaat
      841 cgggtactca atcaacactt gaacgacact taaataataa acttgtccca acggaaaatg
      901 taggatgccc caaatgaaac ctgccagcat ccctgccttt gtccctcttc ttaacgcaaa
      961 aagagtcaat ggaatctgcc ctaatgaaat agaaaaactg ctgccgatct ggatcggaat
     1021 caacgacaac accatcgcta acgctgcaac aatcgttcct tctacccaaa ctctattttt
     1081 tcccataaaa aaacttcctc ctttgaataa aaaagtcaca aaggaggaag cagctttcga
     1141 aaatagtcaa acagctgttt attcacaatt cctacgctcg cataaccgat acaggtacga
     1201 agggtttaga aatagattca atctcagccc aattggactc ccctttgtga gtttatttat
     1261 tatttatttt ttgtttcaaa caatggacta actggtttgt tttcatggat acgtttgatt
     1321 gcatctccca ttagtccgcc aacacttact tcatcgattt tgtcgatttt acgatcttct
     1381 ggaagataga tcgagtctgt aacaactaga cgctcgatag ctgaatcttc tattctttgt
     1441 aaagcagggc cggataatac tggatgtgta catgaagcat acacactagt tgcaccagct
     1501 tctttcaaag cattggcagc caatgtgatc gtacctgctg tatcgatcat atcatcgatc
     1561 agtacacaag ttttgccttt gacttgtcca atgatattca ttacttctgc aacatttgct
     1621 tttggacggc gtttgtctat gatcgcaatc ggagatttca aaaattccgc taatttacgt
     1681 gcccgtgtga caccaccatg gtctggtgaa acaacgacaa catcatcgcc tttgattcct
     1741 ttttctaaga aataattggc aataagtggc gcacccatca aatgatcgac tggaatatca
     1801 aagaaacctt ggatttgaac tgcatgaagg tccaaagtca gcattcttgt tgcaccagct
     1861 ttttgaatca tgtttgctac taatttcgct gtgattggtt cccgtgcacg tgctttacgg
     1921 tcttgacgcg catagccata ataaggcatg actacgttga ttgtttttgc acttgctcgt
     1981 ttcagtgcgt caatcatgat cagcaactcc atcaagttat cattgactgg gctgctcgtt
     2041 gattgaataa catagacatg cgatccacga atactttctt caatgtttac ttgaatttct
     2101 ccatcactaa attgagtaac agatgatttt cctaactcga cgccgactgc tgctgcaatt
     2161 ttctctgcta atgggcgatt ggaatttaac gcaaaaattt ttaatcttgg gtcaaagtaa
     2221 tgttttgaca tggggacctc cactttcttt ttcacaactc tagaattcta cctaataaat
     2281 agtagtcata ttttttaaat aaatcaagtg attttgcaaa agtcttagtt tagataaggg
     2341 agtttttttg catatccttc tttattgact tgtcttgctc tcgcaattgc cagcgcatat
     2401 tctggaatat tgtctgtaat tgttgacccg gcagcaatcg aactgttttt agctacctcg
     2461 acaggtccga tgatattcgt gctagaacca ataaagctgt ggtcgccgac agtcgtatga
     2521 tgtttgttct ttccatcata gttgacgaag acaacaccac atccgacatt gatttcttct
     2581 cccagcgtag catcaccaac ataagtcaag tgaccgactt tagttccttt gccgatttga
     2641 gcatttttca cttccacaaa gtttccgata tggacgtttt cgccaatttc agcttttggt
     2701 cgtaaatgtg cataaggtcc cacatctgca tgtttcttga catggctgct ttcaatgact
     2761 gagttttcaa tgacgacgtg atcctcaatc ctactgtcta cgatttttga atgtgacccg
     2821 atgacacaat cagaaccaat gactgtattt ccttggattt gtacacctgc ttcgatcaat
     2881 gtgtctggac cgatttctac gcctgcatcg atgtatgtcg ttgctgaatc gacaaagctt
     2941 acgccattga ccatatgcat ctggttaatt cgctttctca tgatctcatt tgctttagct
     3001 aacgcgatcc gatcattgac tcccattgat tcttcaaaat catccgtttg ataagcagca
     3061 actgtatggc cttcttcttt taagatttcg atgatatctg ttagatagta ttcaccttga
     3121 gcattgtttg ttccaacttt gcttaacgca tcgaacaaag cttcgttgtc aaaacagtaa
     3181 gttcctgtat tgatttcttg tacaagagct tcttctggtg ttgcatcttt ttgttccacg
     3241 attttttcta cgatcccaat atggtcacgg atgattcgtc catatcctgt tgggttttct
     3301 gcttgtgccg tcaagatcgt tgcacttgca ttttttcctt ggtgatattc aaaaagattg
     3361 ttcagcgttt cagtagtcaa taatggtgta tcgccactga taactaacgt tgttcctttt
     3421 tttccttcca agaaagaagc agcttgcaaa acagcatgtc ctgttcccaa ttgttctgct
     3481 tgtaacgcat atttactgcg ttctcctagt tgtgctttaa cttgttctgc tccgtgtcca
     3541 acgatcgtaa tcacttcatc tggtttcgtt tcactgaccc gattaatgat atgttctacc
     3601 ataggtttac cagaaacggg atgtaaaact ttatataatt ttgacttcat gcgggtacct
     3661 ttccctgccg ccaaaataat tgcataacgt gcgtccaact gcgtcactcc taaatatagt
     3721 ttgctgattt cagtttatcc cagtataccc attttttcaa caaaacaagt gattttcttt
     3781 tatcccagcg ttttctggat aaaaaaatag atagaccata acgggcctat ctatttggta
     3841 accggtgctc tcctgccaac tcctcggtca caagtcacac tactcataaa acatgaaaag
     3901 ctgttttttg aaatcgtttc ttgtgactta gagttaaacg gtaggcttac aacctcttca
     3961 ttaaacgttt ttcaatcagc tgaccttcgg tattagctca ataaacggca aaatatgagg
     4021 agcaattttt ccgtttattg agctaatact caggacagga cgctgatttc acaacctcta
     4081 gataaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatga aaaaatgtgg
     4141 aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcagctttt tcacaacctc
     4201 ttattctgaa aaataatttc ctggcacaac cgtaatattt ttcgtttgag tatctacatc
     4261 tttgacataa agcagtgacg tataatcatc gatggcgcga tgaccgttga attttgattc
     4321 ggcaaataca gtgatgccaa caagttctga ttcgaattct tcaatcaagc tcttcattcc
     4381 gttgactgtt ccgccgcctt tcatgaaatc atccactaca aggacacgtg aaccacgttt
     4441 caaacttctc ttagacagtt ccatcttttc aattctttca gaagatccag acacatagtt
     4501 gacacttact gttgatcctt ctgttatttt tgagtcccgg cgtacaataa cgaatggcgc
     4561 attcagataa taggacacag cttgggcaat aggtacgcct tttgtggcta cagtcattac
     4621 agcatcgatt ttttcaccaa ggtatttaga agcaatgatt cttccaactt gacgcagcag
     4681 ttcaggttct cctaacaaat cagagagata aacatatcct cctggaagca gacggtcttg
     4741 ttctgataga cgttctgcta gagattcaat atattttttc gcctcttcat atgaaatttc
     4801 agggatgaac aatacaccac ctgcagcacc cgggattgtt tccaaggttc cataccctcg
     4861 ttctttaaat gttttcttga tgattcccaa atcttcactg atagaagatt tagcagattc
     4921 ataacgttcg gcaaagcttg ttaaggaaat caattcatgc gggtgattca ataaatactg
     4981 tgtcatgtca actagacgtt cactgcgacg tactttcacc tattccaact ccttttaaga
     5041 aattttatcg ttttacatta tataagtttt tcttcaaaaa ttcgagcatt ttatgaaaaa
     5101 ataagaaaat agttcggtat ttattaaaat aaaaagaagc ctataaaaaa tttttcgttt
     5161 ttataaggct tcttttcttt ataatttagt tattttttct tctgaaggca accaatattc
     5221 gtttgatgat atttacaatc aggaacaaag caataaagac taatgtgatt gttgctcctg
     5281 gaggtgtatc tagataaaac gagctagtta gtcctgacag catccctaga aatcctacac
     5341 aaacactgat agcaatcacg acattgaagc ttttgcctaa tcgcataccg attgctgcgg
     5401 gaagaaccat gattgcggat atcaaaagtg caccggcaat cgggatcatc acagcaatag
     5461 ccacaccagt aatcacattg aacatcatcg acatcaaatg aacgggtagc ccatccacgt
     5521 gggcggtatc ttcatcaaaa gtcagaacat acatcggccg tttgaataaa aagaacaata
     5581 taccgatggc aacgaataag attcctaaaa agatgacttg ctcccatgta atggtcacga
     5641 tcgaaccaaa cagataggat tggatacttg ttgctgaatt ccctccgctc aaattcatca
     5701 atactaacgc tagcgccaaa ccaccagcca ttaaaatggc aatggagatt tctgaatagg
     5761 ttcggtagat cgttcgcaga tactccaata tcacagctgc taatacaacg actagcatgg
     5821 tcgtaagtgt aggattaagg ttcaaaaaga atcctaacgc tacccctgct aaagagacat
     5881 gagaaagcgt atcagccatc agcgattgtc ttctgatcac taaaaacaca cctagcatcg
     5941 gggcgattcc ggcaatgaag atagcggcaa gaaacgctct tctcatgaat tcatatgaaa
     6001 gcaacgccat tgagaatcct cctttctcac cagtcggatc tgtctgtccg catactgttt
     6061 gatatcttca tgatcatggg tgatcattag aactgatttc ccatggacat gcgcactatg
     6121 tcggagaaga cgataaaatt catttctgga ttcttcatcc atacctgttg ttggttcatc
     6181 aagaacaaat aagtcagggt cagtagcaaa aatacgcgct aaactgattc gctgcttttg
     6241 tccgccagac aattcaccta ttcgccgatt tcgcatatcc cacatcccta cagcttctaa
     6301 agcacgctgt acatgttgat gatctttttc agagagacgc ttgaaccagc ggcctcttgg
     6361 atatcttcct gaacggacta attcgatcac cgtactagga aaacctgcat tgaaagatgc
     6421 aacttgttga ggaatataac cgatacttaa tttttttcct tcttgatttt tctcagcaat
     6481 ctttatcatt ccttttgaag gtttcagtaa tcctaacgat gctttcacca aggttgactt
     6541 ggctgctcca ttttctccag ttaaaatgac aaattcaccc gcatctacat gataagagac
     6601 atcttctagt acaggttcat catcatagta aaaggtcaaa tcagatattt cgatataacg
     6661 caccttacaa cctccattct aaaacttaat tgatactttc ttttaaagct ttcagatttt
     6721 tttccatgac agaaatataa ttttcgcctg ctttgatttg ttcattagtc aagctttcta
     6781 aaggattcaa cacttctagc ttcactcctg tttcattcga caatgtctca gctaccttcg
     6841 aagaggcatt ttcttcaaaa taaatcactt tgatgttgtt tttcttcaca aattctttta
     6901 gctcagctaa gcgacttggt gtgggttcct gatctggtga aacgcccgaa atcgctactt
     6961 gttttaagtc gtattgattc gctaaataag caaaagcagc atgctgagtg acaaatgtgc
     7021 ggtttgttgc atcttttagc tcatcggagt atttttgatc cagtgcttca agctttttga
     7081 tataagcatc ggcattcttc gtaaaagttt cctttttatc tggatatttt tcgcctaatt
     7141 cttcagcaat cgttctgact tcttttatag ctaataccgg atcgagccaa acatgtggat
     7201 cgtattcatg tgaatgacca tgcccttctt ctgattcttc atgttcgtct gcttcttgcg
     7261 attctaaacg gtcgattcct ttagctgctt cgatcatcaa ggtctgtttg ctatccactg
     7321 aatcctttaa cgaatccaca aaaaattcca tgtctgaact attatacacg aaaacatctg
     7381 catcggaaat ttctgctctt tcttttgcac ttggttcgaa atcatgcggc tccgttccag
     7441 cagggatcaa aagtttcacg tttccttcat cccctacgat ctcttttgta aaatcataca
     7501 tcggataaaa agtcgtaacg atcgtcaaat cctctttttt gtctgcttcc ttgttcgtat
     7561 ttccacatgc tgcaaacagt aaagcaccta tcattaccgt agctgtcaaa agatattttt
     7621 tcatttatat gctcctttca atgaacaaat cggaatttta cgatttatta acgtgaacta
     7681 atgtatcaaa ataaaaaaac acagtcaata gaaatccatc aatttctgat ttaagcatgt
     7741 atattcttga ttaaatcgta aaatggatga gttgctgctc atatctagcc atttcactac
     7801 aataaaaaac tgaagaatac cattttagga ttcctcagtt tttcatcacc tattttactt
     7861 cttatatttt attgcagggt tcgtactaaa taaacttcct cacaaaaacc ttttaaccca
     7921 ttatatactc tctgcgctct tgaataatgt tgacacaagg caaaaacggt tggtccgctt
     7981 ccactcatca atgcagcatc tgctccgtac ttgatcatac gctctttgat ttgatggatg
     8041 actggatgcc gctttcccgt tatatcttct aaactgtttc ccattttttt gatcatctgc
     8101 tgataatctt gctctcggat ggcttcagca agtccgtgga tgtcttgatg ttggatggct
     8161 tgcgtatcta cttctctaaa cactgtacgt gtagagacac tcattcttgg tttgaccaac
     8221 acaacccagc actgtggcat agaaggtaat acttggattt cttccccttt gccaccgaca
     8281 aatgccgtac ttccgtagac acagtaagga acatctgtac cgacttccaa accgatttct
     8341 gccagttctt ctaacgaaca gcccagattc cataattggt tgatccctct tagagcagct
     8401 gcacagtctg tacttcctcc gcctaatcca gctgctactg gaatattctt cttaacaaag
     8461 atcttgacac cggttttgat attgtattta ttttttagca ataaagcagc ttgataaaca
     8521 ttgttccgtt catctaccgg taaaaatgct ttatttgttt ctacaatgat tttgtcttct
     8581 gttatttctt caaaaaatag atgatctgcc aagtctacgc tcgacatcac catttccaac
     8641 tcatgatacc catcttgccg tctatataat acatccagtc ctaaatttat cttggctggc
     8701 gctttttcaa tcaattccat cctctcaccc attcgaaaag tctaagtcgc aagtattcta
     8761 tcatagggaa aaataaaagt atagcttatg tctgcctagt ggttgatttt gtcatcgttt
     8821 caacagcttt cacagaattt tctcttcata atccaaacaa gttctagtga ttcttataat
     8881 tctgtgagca cttcttctgc caaactcgga acatttcctt tttcttttcc tatcagactg
     8941 agtagctgca tggtagcaaa tccgaaatta cttcctttcc tataagcaaa ataacgtcct
     9001 tcccaatgat cgacatattt taatttcttt tcataagcag actggaagga atagaaaaaa
     9061 ttaccataat tatatacgat atgcatgact ttttcttgaa agaaagaaaa atctgtttct
     9121 ccaacattag ctaatggagc taagcctaga ttggcttccg agtagcccat tttctcgtat
     9181 acaataaaca gattggctaa cagatagtca gctaattcct cagattcttt aggtaagacc
     9241 cgcaacaaat catatcccgc ctgcttcttc gtcatcggct gagcggtgat gaagccaacg
     9301 actttttgat ctttacggac aataccgaca ccgctagata atacatatga tttatcaaat
     9361 cgtcccacag agaagtattt ttctcgtgca ccttgaagcc atttttgtga aacttcattt
     9421 acagcttgat gtaattcttc cggcagttga tcgtaccaag taaaatggta tccttctttc
     9481 atcaaacgtt gatattcaag ttgctgactc agccgagaca agtcttctgt tgctagatca
     9541 acaatccctg cttcacctac tttcgtgaag tgaaaaccca attcatgaag catcactacg
     9601 tagctttctg atatcttata aaacgcaagc tgatagccta aacaatctgc ttgtttcata
     9661 aactgattcg ttgctgcccg catcatttta ggattgccaa ctggatctcc taaaacaaag
     9721 catttgtttc ctttgatctg ataaccaaaa acgacttgat cttggtcgtc ttcttggtaa
     9781 taaaaataac tgtaacctga caagcgaagc tgatggcttg tagctgtgcc tccgtatgtt
     9841 tccaataaac ggtcaaaacg gtctttttcg tactcactgc caagcttcgt agtatcttca
     9901 gctaaatatt gataaagcgc cactaaagct agcaatgaga tgcccaaacc aataagccct
     9961 gagaaccaca catcatctga aggaaacaaa ataaaatgat ttcctgttat tcgaccgact
    10021 aaaaagcgtc cgctgtgata gcctgcaatt gcgtaaacga taaacaaaaa gccgaacaag
    10081 ctagcatcaa agattatagc tccccaagaa taaacgaatt ttgttcgata aaattctttt
    10141 ctggataacc acacagctaa cagaatcaag acatatacca atatcaactg ccaagataac
    10201 gtacgtgtga ttgtatttaa aataccaaaa gatagtaaag caatcgtagg cagaaatgcc
    10261 tttttgactt ttactgaaag tgctctagca aggccgagta ataaaaagcc aaccaataga
    10321 ttcactgtct gatctaaaaa attgaatgag aacggcaata atacttgaaa tagccgactg
    10381 attgcagaca gattagtgat tgttgataga agtaccatca tgatccccgc aaaatagaca
    10441 gcacttacga gcacgatatg cgcaaccttt tggaaaaaca gtcgcggaat gttatctaaa
    10501 aatcgattga ttttgactcc tgtatgtgtg atgaacaata gaagtcccgt cataaaaggt
    10561 agaacataat aaaacaaccg ataatataat agccaaacta ctgctacatc ttgccgaaca
    10621 ggcaattgac tcattcctaa gatcatcaaa acgtcaaaag tccccattcc acctgggacc
    10681 atcgtcagca ttcctatcaa tgtagcaatc atgaacatag gatagatcga aagcatcgaa
    10741 atatctattc tcatcaactt gccgatgatc aaaaataccg caagggctcc cgtccattgt
    10801 ccaaaggatg ccagtagcag accaagcaag ccttttggat aaaactcgaa gaacaatgtc
    10861 tttctcttta gataagcaaa aaagaaaatg actggtgcta aaaaactacc gccgaaaagc
    10921 cagatccaat agcttcgaaa aatactttct gactgaataa aaaagacatc aataaatgta
    10981 agaaacgacc atacagacaa accagtcagc ataaatagtg cgacttttga tacagtggct
    11041 aaaaccattt ttctatttgt ctgctttcca taaaaactag cccgcagact tgctcctacc
    11101 acaccaccaa aaccggcgag attgttgatc gtgtttgtta cccacgcagc aagaagaaac
    11161 tcttttcttt ccatttttgg ttttccttgt ttttctaaga ctctgatcgt cacccaatca
    11221 tacaacaaca tcggagtgac acccagcagt ccagccagaa tcatgagcag gatcgtcatg
    11281 cggctttgtt gtcccatcct ttgccaaata tcttgccaac tcatgccatt agcaatattt
    11341 gcgacttgat ttgccacaaa gactaacacg gagccgaaaa aaatcagttt taaaatcaga
    11401 ctatgttcct ttatccattg cagcatcaag tatcgtttat ttttcacatt ttcccattcc
    11461 taacttgtat acttttgtgt cataccccaa tgttatctta tttattttaa tttttccact
    11521 ttagacctgt tctttgcttg cgatgataga aaaagaaaga tatcctaatg ctaaaggaga
    11581 ggataacatg aaagtaggat ttatcggcac agggaatatg gcacaagcta ttattgatgg
    11641 tttattaaaa aaaggaacag tctcgaaaga agaggtgttc gtttccagtg gacattttga
    11701 aaatgcacaa aaatttgctg ctgaaactgg cgtccatgca tgcaaaagta atcaggaagt
    11761 tgcatcacaa tcagatatcg ttcttttagc cgtgaaacca aaaatcattg aaaaagtatt
    11821 ggaagaacta agagaatata gccagaaagt cttattcatt tctattgctt caggaaaatc
    11881 attaacagat ttagaaacag cgcttgaaga tcctcaggcc tccatcgtac gtgtgatgcc
    11941 gaatgtcaat gtcagcgtag gagcaggcgt ttctgcgatt tgcaaaaatg aagctgccac
    12001 ggaggaacag cttcagcaag ctacgatgat ttttgaagca atcggcagcg tctatccttt
    12061 agcagaatca gatttcagta cattcattgg ccttgctggc agctcacccg cattcgttta
    12121 catgctgatc gatgcgatgg gacggactgg tgtactgaat ggtttgtcca aacagacagc
    12181 cactgagatt gctgcaaaag caattatggg ttcttgtcaa aaattccttt caagtgataa
    12241 aaatccatgg gaacttgtag accaagtttc ttctcctggc ggtacaactg ttgcaggtgt
    12301 agttgcctta gaagaagcag gattcatccc agcaatcatc aaaggaatca atacgacgat
    12361 cgagaaagat cttgagatgt tgaataaaaa ataatgatag caatgttttt tcgtgcctta
    12421 caaaaaaaga agagctgtgg cttttgccgc ggctcttctt ttgtattttt tattatttag
    12481 cataatcagt tgcacgagtt tcccgaataa cgattacttt gatgtgtcca ggataatcca
    12541 gttcttcttc gattcgttta cgaatatcac gtactaaacg aacagcgtct aaatccgaga
    12601 tttcttctgg cttgaccatt acccgaactt cgcgtccagc ttgaactgcg taacttgatt
    12661 ctacaccggc gaagctgttc gaaatatttt ccagacttct taaacgacgg atgtagtttt
    12721 ctaatgattc acttcgcgca ccaggtcttg ctgctgagag cgcatctgct gcagctacaa
    12781 ggacagagat aacagaagta gcttccacat ctccatgatg agaagcgatc gcgttgatta
    12841 ctactgggtt ttctttgtac ttggcagcta attccgcacc gatctcaacg tgagatcctt
    12901 caatctcatg gtcaagtgct ttcccaatat catggagcaa cccggcacgt tttgctaatt
    12961 ggatatcttc gccaagctct gctgctaaga taccagctaa ttttgcaacc tcgaccgaat
    13021 gcttcaagac gttttgtcca taactagttc tgaatcgcag acgtccgatg atcttgatta
    13081 gatctggatg aagtgtatga gcaccgactt caaacgctgc ctcttccccg tattcgcgaa
    13141 tacgttcatc catttcttta cgagatttct cgaccatttc ttcgatacgc gctggatgga
    13201 ttcgtccatc ttgtatcagt ttttccaagg tcatccgtgc gatttctctt cggattggat
    13261 cgaatccgga aagaacaact gcttccggtg tatcatcgat gattaaatcg ataccagtca
    13321 gcgtctcaag cgttcgaata ttacgacctt ctcgtccaat gatacgacct ttcatttcat
    13381 cattcggaag ggtaacgact gaaacagtcg tttctgaaac ttgatctgct gcgcagcgtt
    13441 gaatcgctaa tgataataag tttttcgctt tgcgatctgc ttcttctttg gcacgctgtt
    13501 cagattcttt taccattaga gttaactcat gattcagctc ttcttcagtc gatttcatga
    13561 tgacatctcg tgcgtcctct tttgataaac cagcaatttt ttctagttct ttttgttgat
    13621 cttcaatcat tttttcaacg tctttttctc gctcttcaat taattgctgt tttgaactaa
    13681 gtgcttcttc tttactttca agtgagttct cgcgtttttc taacgagtca tctttacgat
    13741 tcagcgtttg ttcgcgttga atcaaacgat tctcttgaga ttttaattct aacttacttt
    13801 ctctcagctc actttcaact tcagaacggt atttctgatt ttcttcctta gcttctaaca
    13861 atgcttcttt ttttagagtt tctgcttctt tttttgcact atcgatgatt ccagcagctg
    13921 atgattgtgc accagctatt tctttctcgt gacgtgactt tgcgaccata aaccctaaac
    13981 caagaccgac aattaaaccg atgatagcga ggagaatgtt gaataccata aaatccacct
    14041 ccatactatc tttttcattt ttatcatcta tacagcttct atgataaatt attctaaact
    14101 cgtcaatatg catcttcgca tatatgtgta caaaaaacat gcacaacttt attctaatgt
    14161 ttgtaccccg tagtgtcaac tttaaataaa caaagaaacg gctttctact tattcattaa
    14221 acgatg
//
LOCUS       DAZKNO010000063        14074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000063 DAZKNO010000000
VERSION     DAZKNO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.064"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            182..496
                     /locus_tag="ACG8MY_001418"
     CDS             182..496
                     /locus_tag="ACG8MY_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0430614.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            517..894
                     /locus_tag="ACG8MY_001419"
     CDS             517..894
                     /locus_tag="ACG8MY_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0430615.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            904..1677
                     /locus_tag="ACG8MY_001420"
     CDS             904..1677
                     /locus_tag="ACG8MY_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430616.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1699..3102
                     /locus_tag="ACG8MY_001421"
     CDS             1699..3102
                     /locus_tag="ACG8MY_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0430617.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3284..4468
                     /gene="mobT"
                     /locus_tag="ACG8MY_001422"
     CDS             3284..4468
                     /gene="mobT"
                     /locus_tag="ACG8MY_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0430618.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4465..4755
                     /locus_tag="ACG8MY_001423"
     CDS             4465..4755
                     /locus_tag="ACG8MY_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430619.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4752..4973
                     /locus_tag="ACG8MY_001424"
     CDS             4752..4973
                     /locus_tag="ACG8MY_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430620.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5015..5794
                     /locus_tag="ACG8MY_001425"
     CDS             5015..5794
                     /locus_tag="ACG8MY_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFQ0430621.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            5855..6355
                     /locus_tag="ACG8MY_001426"
     CDS             5855..6355
                     /locus_tag="ACG8MY_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0430622.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            6411..7049
                     /locus_tag="ACG8MY_001427"
     CDS             6411..7049
                     /locus_tag="ACG8MY_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFQ0430623.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7121..7516
                     /locus_tag="ACG8MY_001428"
     CDS             7121..7516
                     /locus_tag="ACG8MY_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0430624.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7500..9953
                     /locus_tag="ACG8MY_001429"
     CDS             7500..9953
                     /locus_tag="ACG8MY_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0430625.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            9950..11977
                     /locus_tag="ACG8MY_001430"
     CDS             9950..11977
                     /locus_tag="ACG8MY_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0430626.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            11974..12996
                     /locus_tag="ACG8MY_001431"
     CDS             11974..12996
                     /locus_tag="ACG8MY_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFQ0430627.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13013..13942
                     /locus_tag="ACG8MY_001432"
     CDS             13013..13942
                     /locus_tag="ACG8MY_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0430628.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         4768 a         2383 c         2806 g         4117 t
ORIGIN      
        1 gttttaaata tagtaggaat cggacgctca gtacggagcg tctatttttt tacccaattt
       61 tgaaaggaag tgaacttatg aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct
      121 ttaaaaccat cacacaaata agattcattc aaacataaaa acagaaagga taggtaaaaa
      181 tatggaactt aaatatgtca ttcccaatat ggaaaaaaca tttggacaat tagaatatgc
      241 tggtgaaggg aacattgaac aacgtcgaat aaatggcaga ccaaccattc tctctcgtag
      301 ttacaattta tactcaacga ttcaacgagc agatgatatt gttgtcattc ttccctccga
      361 agctggcgag aaacattttg aatccgacga aaaagtcaaa ctcatcaacc cacatattac
      421 cgcagaaggt tataaaattg gcaatcgagg atttaccaat tacattatgc acgcagacga
      481 tatggtgaaa gcataagaaa ggaaaggtga aaaaatatga gattagcaga aggtattgtg
      541 attgaaaaag accagacatt tggcacattg aaattttccg ccttgcgtcg tgaggttcgt
      601 atccagaatg aagatggtac ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa
      661 tcaaaaggac aaggacgcat gatacaagtc agtatccccg cttctgttcc tttgaaagaa
      721 tttgactaca acgcagaagt ggaattaatc aatcctgtgg cagataccgt agcgacggct
      781 acttttcgag gggcagacgt tgattggtac gtgaaagcag acgatcttgt attaaaagga
      841 cagacaaaac ctcatcagaa atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg
      901 cttatgacaa acacaccatt gattttgaaa gaaattccga aggatgaagc catttctttt
      961 attcggcaat accattattc aaagatactc cctagattgt gtaaatactt tctagggatt
     1021 ttttcagaag aaaaattatt aggcgttgtg gaattaggct ggggtacgca acccctacaa
     1081 accatacgca aactctttcc cgacagttcc cttcaaacca cagattattt agaaattgga
     1141 aagatgtgtt ttcttccaga aatgaatcaa accaattatt ttggcagtca agctctttca
     1201 gctctaatta agtggttgaa agaacatacg gactgtcatt ttttatatac actggcagat
     1261 ggaattgagg gaaaatgtgg ctatgtctat caagcgtcca acttctttta ctgcgggtat
     1321 ttcaagacaa gtgtctatcg tgataagcag tcatgggaaa agatacaccc tcgaagtgct
     1381 cgccttttat tagaggaaaa tgctcgcttt gaacaggtag agaaaaagca ttggctttcc
     1441 caggcatttt gcgaatacaa agggattgag aaaatcaatg gacgcatgtt tcgctatctc
     1501 tatccgttga caaaagaagc gaaaaaactg ttaggacaca ctctctatcg acgtcattat
     1561 tatccgaaag aaaaaaattt acgctttgaa aagcgaatcg cttatcagaa atatgaagcc
     1621 atttctcaac caacttttga taaacaggct cgtatctaca atacacaatt attttgatag
     1681 gtggaaagga ggatttttat gccattgctt catgacagag gagagcgaat caaagccaac
     1741 gataagcact tggtctatca ttttgtacta atttcattgc tggtagtttt tctagtcgtc
     1801 ctactccctt ttcattggag agcgttacaa tcggtgaact ggcaagcagt tcagttagat
     1861 acatttttct atcaacttca tacgccatac ttttggagca gtattctcac tgctcttttt
     1921 gtatgcggac ttatcagctg gctttattat cactatcgta tagattctat aaaacagtta
     1981 gagcaccgtc aaaagttagc tcgtatgatt ttagaaaatc attggtatga agccgaagaa
     2041 actcaatcag agggattctt taaagattta cccagcaaga aaaccaaaca acgaatccgc
     2101 cattttccta aaatatatta tcgaatgaaa gatggactaa tccatattca tgtagaaatc
     2161 accttaggga aatatcaaga tcagcttttg cacctagaaa acaaactaga aacaggattg
     2221 tattgtgaat tagtggaaaa aacgttgaaa gattcttatg tagagtatgt cctactttat
     2281 gatactattg gcaatcgaat tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa
     2341 ttgatggaat cggtctattg ggagttcgat tctctccctc acatgttaat tgctgggggc
     2401 actggtagtg gaaaatccta ttttattctc acattgattg aatctctcct gcatacaaat
     2461 gcaaagcttt atattcttga ccctaaaaat gctgatttgg cagacttagg ggcggtcatg
     2521 aataatgtct attaccgtaa agaagatatg ttggcatgta tcaatcaatt ttacgaagat
     2581 atgattgatc gcagtgaaac catgaaacaa caccctaact ataaaacagg agaaaattat
     2641 gcttatcttg gtttgtctgc caattttctt atttttgatg aatatgtggc aatgatggat
     2701 atgctaggtc gtgaaagtac caccgttatt cataaactca aacaaattgt aatgttagga
     2761 cgacaagcag gcttcttttt aattcttgcc tgtcaacgtc cagacgcaaa atattttagt
     2821 gatgggattc gggacaactt taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc
     2881 tttggtatga tgttcggaag cgatacacaa aaacaatttt tcttaaaacc catcaaggga
     2941 agaggttacg tggatacagg aaaaagtgtg attagtgagt tttatacacc ccttgtacca
     3001 aaaaggtacg atttcttagg agaaatagga aaagtcattc aaaaaaagca atctgagcct
     3061 gtaccgcacg aagtgaaagg tacaggttca gacttgcttt gattggtgtg gcgttagcca
     3121 cgccaatcct ttagccccct ctatctaaca ggggggcaca aatacaactg aaaacaggca
     3181 attctcatca accaatgttg atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact
     3241 caagggtcat agtttacatc ttttttagat tggaggtttg cgaatggaaa acgaaaaaat
     3301 ttggattaag gaattgaaag aaaagcgact ggcttatggc gtttctcaaa ataaactggc
     3361 ggttgcttct catattacca gaccttacct atcggatatt gaaacaggca aggcagtccc
     3421 tactcaacag gtgaaagaag atttactgaa cgctttggaa cgcttcaatc ccgataaccc
     3481 attggagatg ttattcgact atgtacggat tcgattccct accaatgatg tggctcaaat
     3541 tatcggtgag gttttacgtt tgaatatgga ttatatgcta cacgaagact ttggctatta
     3601 ttcttatccc gaacactatc gtttcggtga tattgttgtc ctagtctctc atgatgtaag
     3661 taaaggtgtt ttattagaac tgaaaggaaa aggctgtcgg caatttgaaa actttctatt
     3721 ggctcaacat cggagctggt atgatttctt tcaagattgc atggaacata aaggtatttt
     3781 caaacgcttg gatttagcta tcaacgataa aacagggatt ttgaatattc cagatttggc
     3841 aaagaaatgt aaacaggaag aatgcatttc cgtctttcga agctttaaaa attatcgcag
     3901 tggtgaattg gttcaccgtg atgaaaaacc atacatggga aacacacttt atattggctc
     3961 attaaaaagt gaagtctatt tctgtatata tgagaaagat tatgagcaat acgtgaaaaa
     4021 tgatgttccc ttagaagacg cagaagtaaa gaatcggttt gaaattcggt taaaaaatga
     4081 ccgagccacc caagccatga aagatttatt ggcacatcaa caggcggaaa aaacagcctt
     4141 tgaaattatc aatcgctata tccgctttgc agataaagac gacactaaac gtcggagtga
     4201 ttggaaaact aatgaacgtt gggaatggtt tatcggtaaa aatcgtggag cattacgcct
     4261 aaccacacag ccagagcctt attcttttga acgaacattg aactggttac atcatcaagt
     4321 cgcccctaca ttaaaaatcg caagcatttt agatgttctc aacggtacaa ccattatctc
     4381 caccatgatt caggaagcca aactgacaga aaaacatgag aaattaattg aacaacaaca
     4441 tctagccatg gaggatttga ttacatgagc cttttaacaa aagaattaaa gaagttaggg
     4501 tttcagtgtg gcattgagtt tcaagcgtat atacaaaata cagggaaata tacgtctctc
     4561 attattgagg gaaaacggca agctggtgat accatctata cctatgattt ttacaaggtg
     4621 agattttata ataattatac caatcgagta accgtctatg gagagcattt aacccctttt
     4681 caattattaa ggcgtgtaaa aagctatatc tattatcgag aaaaatatct taaggaaagg
     4741 aggacaatca catgaatttt gggcagaatt tgtacaactg gttcttggat aatgcacagt
     4801 cattggtatt gttagcgatt gttgtcattg gactgttttt aggttttaaa cgagaatttt
     4861 caaaactcat tggcttcttg attattgctc taatcgccgt cggacttgtg ttcaatgcag
     4921 ctggcgtgaa agatgtgtta ttaaacttat ttaaccgaat cattggtgct taacagatat
     4981 ttttcatgat aaaataggaa gtatatattt tgttatgaaa ggatttatga atatgtttac
     5041 aaatgatcaa tttaaatctc ttttaattaa aacattagaa agaaagagct cttcaaatta
     5101 ttatgaagga ggtaacgaga ttgtttctaa aatgaaaata tcagaggtaa ctttagatga
     5161 gactgacgat tttacttatt ataaaggata taaaaagggc tggaatacgc tttgcacata
     5221 tcttaaaata agagttcctt ttgatgattt agactttttt gaaagtcata aagatttgat
     5281 tacccagaca gcaagctcca tatatgataa acaaggtgat aatgtattag tggatactat
     5341 tttagtacca cttccagaga attacgaagt aattaacttt agtcagttga aaattagtga
     5401 tgtggtttca caagctattg aagacgctga gtcttttatg agtaatggtg aatatcaacg
     5461 tgcctttgac agagttcaca ctgcttttca tggatattta attgaaatac taaaaaaata
     5521 tgaaattaca gttcctagag acgaaaatct atctaaatta tatagtagga tacaacaatt
     5581 aattgaaaaa gaaattcaac ctactgaact tgctgatata gtgaagacaa cgataaggtc
     5641 atcaaatgga atgattagct ctcttaatga agcaagaaat aggcattcac ttgcacaccc
     5701 taatacaaat attattggaa agagagaagc aaaactaata ataggtattt caagtacagt
     5761 tacagattat attagcggat accttgataa gtagcgtcat taactgattg ctactttttt
     5821 tatgactatt tttataaaaa ggatgtgata agaattggaa atgaaagtct atgtggcaaa
     5881 tttaggacgg tataatgaag gtgaattagt cggagcatgg tttactccac caattgatga
     5941 ggaagaaatg gcagaaagaa tcggtttaaa tgaggactac gaagaatatg caatacatga
     6001 ttttgagctt ccttttgatg tggacgaata cacgccgatt agtgaaatca accgtctatg
     6061 tgaagccatt caagaaatcg aaggtacacc tatttataat gaactcaaag aaattcaagg
     6121 catgtggttt agtagcttgg aagaattact ggaaaacaaa gaggatattc attgttatag
     6181 tgattgtgat tcgatggaag atgtcgcccg ttattacgtg gaagaaacag gacagcttgg
     6241 tgaagtccct tccaatttac aaaattatat tgactaccaa tctttgggac gtgatatgga
     6301 aatagagggt aattacctag ttacctctca tggggtattt gaatattgtc agtaagttta
     6361 acaatccgtg ttataattaa attaataaat agcacggaga ggtataaatt atggtaaatg
     6421 tatttgaaaa tcttaaacta ttaaaactag acatgaaaga taaaggctgg gtgatagatt
     6481 ccttttactt tcgttacaaa cagcaaaatt atattgtact tgttaagcta tttgaaaaag
     6541 aggaaaaagt tccagaatat gctctgttaa aactagaatt tttaaaggaa aacgattttt
     6601 cagatatgct agctgtctat gctaattcgg taaaattata tacagacacg aaaacaataa
     6661 gagaatattt tggtatcgaa tacagtagta acttgggaga tgttttattt caatttagcc
     6721 aaactcttgc tcgatttatt ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag
     6781 aagccatgtg tttctcatta agtcaaagtg attcagaaga tcccagaaaa aaatattgtt
     6841 tttcagttag aagaaatcct ttaaagggaa atggtgagct gggaaaacga agtcctttta
     6901 atgacaataa aacgagattg tatagaccca gcttatatga acgattaggg tcagatacca
     6961 atttaagttt cagatattca atgaatccag atgatgaaga aactgatgaa ggtattattg
     7021 caaaatggac aaaaaataaa attgaatagt taaagcattg aaagagcaat cacctatttt
     7081 gattgttctt tttctatttt tacggaaagg acgtgaagac atgaaaaaaa taagaagtta
     7141 caccagtatt tggtcagtag aaaaagtgat ttatgcgatt aatgatttac aactcccttt
     7201 cccaattacc tttacgcaaa tgacatggtt tatcgtttct ttgtttgtcg tcatactctt
     7261 tggcagttta ccaccacttt ctctgattga cggagcacta ctcaaatact tagggattcc
     7321 cattgttctc actgggttca tgtgccaaaa gacctttgac ggaaagaaac cctatggatt
     7381 tctaaagtct atcattacct atgctctacg ccctaaagta acctacgctg gcaaacaaat
     7441 acgattaaaa aaagaaacaa tgaatgaatc gattacgatt gtaaggagtg aacaactaga
     7501 tggcatatcc gattaaatat atggaagata atctcgtatt taatcatgat ggcgaagtat
     7561 tcgcttatta tgaattaatt ccctataatt acagtttttt aagttccgat gaaaaaattc
     7621 aagttcatga taatttctgt caattgattg cccaaaatcg tgatggcaag attcatgctc
     7681 tgcaactagc aaccgaatca agtattcgag ccacacagga acaatcgaaa aaagaaatca
     7741 ctggacgatt aaaagagata gcttacgaaa aaatagatga acagaccgaa gcgttaattt
     7801 ctatgattgg tgacaatcaa gtagactatc gcttttttat tggttttaaa ctctacctca
     7861 atgaacagga agtatccgta aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat
     7921 tctttcatgg tgtgaatcac aaattaatgg gcgattttgt ttctatgcct atggcggaaa
     7981 tcgaacgctt ttctaagatg gaacagttgt tagaaagtaa attatccaga cgctttcaga
     8041 ttcgtaaatt ggagaaagat gattttggtt atattattga acatctttac gggcaaacag
     8101 gtgtcccttt tgatgactac gactaccatt taccgaaacg atacgaagat aaaaaagtgt
     8161 tggtaaagaa atacgatatt ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct
     8221 atttacggat tgaacaggaa gataccacta cttatgtggc gtattttaca attaatagta
     8281 ttgttggtga attggatttc cctaatagtg aaatttttta ttaccagcaa cagcagttta
     8341 ctttccccgt tgataccagt atgaatgttg aaattgtcgc caataaaaaa gccttatcta
     8401 ccgttcgcaa taaaaagaaa gaactgaaag atttagatag ccacgcatgg gaaagcgaca
     8461 acgaaaccag tactaatgtg gtggacgctt tagaatctgt cgatgaatta gaaagtaatt
     8521 tagaccaaac caaagaatct atgtacaaac taagctatgt ggtacgggta tcagcaaaga
     8581 atttggacga gctgaaacaa cgttgtgatg aagtaaaaga tttttatgat gatctgtcta
     8641 tcaaattagt acgacctttt ggcgatatgc taggcttgca taatgaattt attccctcaa
     8701 gcaagcgata tatcaatgac tatattcaat atgtcacgtc ggattttttg gctggtcttg
     8761 gtttcggtgc tacacaaatg attggtgaaa attctggtat ttacgtcggt tacaatctcg
     8821 atacaggcag aaatatttat ctccaaccta gccttgcaag tcaaggaatt aaaggctctg
     8881 taacaaatgc attagcgtcc gcttttattg gttcattagg tggcggaaaa tcattctcta
     8941 acaatatgct ggtctattat tcagtactct ttggcggaca ggcaaccatt gttgacccaa
     9001 aagctgaacg tggtgaatgg aaagaaacct tacctgatat tgcccatgaa atcaatattg
     9061 ttaatctcac gagtgagaac gacaataaag ggttacttga cccttatgtg attttaaaac
     9121 tcccgaaaga ttcagaaagc cttgccattg atattctcac gttcttaaca ggtatttcca
     9181 gccgagatag cgagaagttt cctgtcctgc gtaaagctat tcggagtgta actcaaagca
     9241 aacaacgagg gttgttattg gtgattgatg aattgcgaaa agaagatacg cccatcagca
     9301 atagtattgc tgaccatatc gaaagctttg tcgattatga ttttgcccat ctgttattca
     9361 gtgatggcag tgtcacccaa tccattagct tggaaaaaca actcaatatt attcaagtgg
     9421 cagatttggt cttacccgat tccaatagct cttttgaaga atacactacc atggaattat
     9481 taagtgtggc aatgctgatt gtcattagta cctttgcttt agactttatc cactctgacc
     9541 gttccatttt taaaattgtt gaccttgatg aggcttggag ttttttacaa gtggcacaag
     9601 gaaaagctct atctatgcga ttggttcgag caggacgtgc catgaatgca ggggtctatt
     9661 tcgttactca aaacgcggac gatttactag atgaaaagat gaagaataat attggcttga
     9721 aatttgcctt tcgttccact gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg
     9781 tggacaagga agatgaaaac aatcagaaac gcttacgtga attagaaaat ggacaatgtt
     9841 tagtttcaga catctatggt cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt
     9901 tgcttcatgc ctttgatacc agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa
     9961 acaaaagaaa tttcttcact atagttggat aacgctcctt tgtgtgggcg ttattttact
    10021 tttattaggc acgctgggca atgcagtaca agcgacaggc ttagttgatg aaaccattga
    10081 tacaagcaat gaatactcca agtatggttt gaatcaatat cagcttgatt attatgtgga
    10141 caatagctgg ggctggcttc catggaattg gagtgatggc ataggacaat cggtgatgta
    10201 tggattgtat gccattacta attttatttg gacaatttct ctttatctat cgaacgccac
    10261 aggttatttg gtgaaagaag cctatgcctt agatttcatt agtcaaacat cccaagcaat
    10321 tggcgaaaac atgcagacga ttgcaggcat tacaccgaat ggcttttcca gttcagggtt
    10381 ttatgtcggt ttcctccttc tttttattct cattgttgga atctatgttg cctatacagg
    10441 attggttaag cgagaaacga cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt
    10501 tattctatcg gcttccttta ttgcgtatgc acccgattat attgcgaaaa tcaatgattt
    10561 ttccaaagat gtcagtaccg ccagtctgaa tattgggaca aaaattgttc ttcctaacac
    10621 ggatagtcaa gggaaagaca gtgtggacat gatacgtgac agcttattct ctgttcaagt
    10681 aaagcaacca tggctattgc ttcaatatgg ctccactgat gtagaagcgt taggggaaga
    10741 acgtgtggaa agtttattgt ctaccagtcc agatacaaac aatggtgaag atagagaaaa
    10801 tgtagtgatt gaagaaattg aagatcatga caatacctat ttaaccctac cgaagacgat
    10861 ttccagatta ggaacagtct tctttttgtt tgtattcaat attggtatct cgatttttgt
    10921 tttccttcta acaggaatca tgattttttc gcaagtgcta tttattattt tcgctatgtt
    10981 tttgccaatc agtttcctat taagtatgtt gccgagtttt gatggcatgg gcaggcatgc
    11041 cattaccaag ttatttaatg tgattatgac aagggctggt attaccttga ttattaccgt
    11101 tgcctttagt atttccacta tgctttattc gctgtcagca agttctccct ttttcatgat
    11161 tatgttttta caaattgtga cctttgcagg gatttatttt aaactgggcg atttaatgag
    11221 cctattcact ttacaaagca acgactctca aaacatgggc agacaagtgt ttcgcagacc
    11281 tcgtcaaatg atgaatcgac aaaccagaag atttcaacgc agtatgagta agatttttcg
    11341 tggaaaagca gggtcaacga agcaaaagac aatgacaaca aagcagaatc aaaccaataa
    11401 agctaaccat actcgtcaaa atgaacggac tacaccaaag aaagaaacag cactttccaa
    11461 tgtgaaaaag caacaaggcg gtatcgggca acgattaggt gaaaagacag cccatgtatt
    11521 ggacacgaaa aatcgtctag tcaataaaag aaagcaggca aaacaacaga tacaggcgac
    11581 accaacccat gtcaaatatg cccttcatca aggaaaagag aatgtcactc ataatgtaac
    11641 agactttaaa caatctatgg cagataccaa agctcaacgc aaacaacagc gtatgaacca
    11701 gcaggagcaa aaacgaaaga acattgctca aaagcgtttg gagctggata aagcaaaaga
    11761 aaaaagacga aaagaaaacc catcatctcc tgttcagaat aaccagcagc atgttcgcaa
    11821 gcgtcagcaa accgtcacgc ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg
    11881 accattgaaa aaagagcaaa aacaaaagac accaccgaag caattcaatc agaggaagct
    11941 aaataaaaga acggataagc gaggacaaca aacatgaagc taaaaataac agtgatttgt
    12001 agtgtggtct tctctctctt cttttttctc ttattattcc tagtcatttt ctttgcagat
    12061 gatacggaca gtggcgaaaa caacaaggat tcttctattc cacaaggcgg tgtgaccgtt
    12121 tcaccagaag tgttggcaca ccgtccgttg attgaaaaat atggtaagga atatgggatt
    12181 gaagactatg tttcttatat cttagccatt atgcaagtgg aatcgggcgg tacagcagaa
    12241 gatgttatgc aaagttccga atctttgggt ttaccgccca acagtttgag taccgaagag
    12301 tccatcaaac aaggggttaa atatttcagt gaattattaa ccagtgcaga acaacaagga
    12361 gtggatatag attctgttat ccaatcttat aattatggcg gtggcttttt aaattatgtg
    12421 aggagtcatg ggaaaaaata tacctatgaa ttagccgaac agttttctaa agagaagtcg
    12481 ggtggtcaaa aagcagacta tcccaatcct atagccatac ctgtgaatgg tggttggcga
    12541 tataactatg gaaatcagtt ttatgttcag ttggtttcac aatacctaac cgacacttcc
    12601 ccaacagaat ttgatgatga aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa
    12661 gggtttccct atgtctttgg aggggcgtcg cctaccacct cttttgattg tagtggctta
    12721 atacaatggg tgtatgacaa agctggcatt tctctccctc gtgtcgccca agatcagtat
    12781 gacgctacgc aagaaatctc tatggaagaa gcccaagcag gcgaccttat cttctttcat
    12841 tcaacctata atgcaggcac atatgtgact catgtggcga tatatttaga gggaaatcgc
    12901 ttttatcatg caggcgaccc aatcggttat ggcgacttaa gcagtcgtta ttggcaagac
    12961 catctgattg gggctagacg tgttattcat aactaagaaa ggaatgaatc aaatgattat
    13021 aaaaatcgag cgaaaagaaa agaaggagaa accaaagaaa tctaaaaaac cgagcaagca
    13081 aagaaaagtt ccgatgatta aagttggcac gcataagaaa ttaacctttg ttttatgggt
    13141 tttattgatt ggtagtgtcg gatttggaat ctataaaaat tttactgcca ttgacactca
    13201 tactgtccgt gaaacagaaa tcattaaaca gcagattgtc gataccaacc aagtagaaag
    13261 ttttgtgaaa tcctttgcga aagactactt ttcgtggcaa caatcccaag aagccattga
    13321 taaacgaaat gaaaaattga cccattattt gacggaagaa ttgcaggtat taaatgaaga
    13381 aatgattcgt aaggatattc ctactagttc tagtgtgaac gatatacagg tgtggcaggt
    13441 ttctcaagtg aatgagaaca cctttgaggt actgttttct gttgaacaag ttattaccga
    13501 agacaaagac aaggaaacta tatcgtctag ctttcatgtg gttgtccata tagatgaatc
    13561 agataatatg gttattatca aaaatccgac aatgagtaag aaacctcaaa aatcggacta
    13621 ccaacccaaa cagctagaaa gtgaccatac agtagataca gaaacgatgg acgaaatcac
    13681 tagcttttta gaaacgttct tccagctcta tcctaccgcc acagaaaaag aattgactta
    13741 ctatgtaagt aatcatgtgt tacctatgat taacaaggaa tatgttttcg aggaattggt
    13801 gaatcctatc tttaccagaa aagataacca agtaatcgtg aatgtcgctg tgaaatattt
    13861 agatcaagaa acaaaggcaa cccaaatctc gcaatttgag ttgatattag aaaaacaaga
    13921 taattggaag attgtaaaat aacaaatatt ggtacatgat tacagatact ttgtaatcat
    13981 gtactctttt tgataaaaaa ttggagattc ctttacaaat atgctcttac gtgctattat
    14041 ttaagtgact atttaaaagg agttaataaa tatg
//
LOCUS       DAZKNO010000064        14060 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000064 DAZKNO010000000
VERSION     DAZKNO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.065"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..610)
                     /locus_tag="ACG8MY_001433"
     CDS             complement(<1..610)
                     /locus_tag="ACG8MY_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0430629.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYL"
     gene            complement(858..1694)
                     /locus_tag="ACG8MY_001434"
     CDS             complement(858..1694)
                     /locus_tag="ACG8MY_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0430630.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1705..2634)
                     /locus_tag="ACG8MY_001435"
     CDS             complement(1705..2634)
                     /locus_tag="ACG8MY_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0430631.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2826..3179
                     /locus_tag="ACG8MY_001436"
     CDS             2826..3179
                     /locus_tag="ACG8MY_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0430632.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3253..3909)
                     /locus_tag="ACG8MY_001437"
     CDS             complement(3253..3909)
                     /locus_tag="ACG8MY_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0430633.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4135..6069
                     /locus_tag="ACG8MY_001438"
     CDS             4135..6069
                     /locus_tag="ACG8MY_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0430634.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6155..7378)
                     /locus_tag="ACG8MY_001439"
     CDS             complement(6155..7378)
                     /locus_tag="ACG8MY_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0430635.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7372..9243)
                     /locus_tag="ACG8MY_001440"
     CDS             complement(7372..9243)
                     /locus_tag="ACG8MY_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0430636.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(9264..10382)
                     /locus_tag="ACG8MY_001441"
     CDS             complement(9264..10382)
                     /locus_tag="ACG8MY_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0430637.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10465..11298)
                     /locus_tag="ACG8MY_001442"
     CDS             complement(10465..11298)
                     /locus_tag="ACG8MY_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0430638.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11291..12208)
                     /locus_tag="ACG8MY_001443"
     CDS             complement(11291..12208)
                     /locus_tag="ACG8MY_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0430639.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12435..13559)
                     /locus_tag="ACG8MY_001444"
     CDS             complement(12435..13559)
                     /locus_tag="ACG8MY_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0430640.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13742..>14060
                     /locus_tag="ACG8MY_001445"
     CDS             13742..>14060
                     /locus_tag="ACG8MY_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0430641.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQE"
BASE COUNT         4411 a         2795 c         2284 g         4570 t
ORIGIN      
        1 ataaatactc atattcgagt gattttttat attttttaat tgtgccgttt acgagaatct
       61 cttcatcttt caatactagg cctattttat ttttataacc tggcatgctc attagataaa
      121 ggatttcatc gtaatggata tagtgatcgc tattaccata acggacagtt gtccaccttt
      181 ttttcgtagc atgttgaaca ggatagtttt ctttttcttt caataagtaa ctttggatgc
      241 tttttttatt tttttcataa gtgaattctt ttatcaaata accactaggc gtaatctttc
      301 gattaataac agttattgct ttattcggat gagacgttag ataaacaatt ttacactttt
      361 cattcttttc tctaagcatt tcgcctagtt cgataccgct ataataggcg ttcaaatcga
      421 tgtctaggaa aaaaatatct gaggaataga tttcattatt tttaatgaat tcaacaaatt
      481 gtataacgtt caatccctga acgatctcaa atttccaagg acttaataaa attctggatt
      541 cttccaaaat ttctactagc attttttcaa aaaccaaact atcatccaaa ataaatatac
      601 gcgttaccat aaaaagtacc tccactcgat attttactat caaaatcatt tcatacaact
      661 tttcaacata catgttagga ataaattgta attctcttca caatcttttc caacaccttt
      721 gaaaccgata tcaaatactt tcaggatgct gcttctaaaa taaatatcaa acaaatgaat
      781 acatcatgca aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc tgagctatgt
      841 ttatttattt tgttctttta aaaagaacga tacacatact gatttttcgg agcagagact
      901 ttctcagcgt ttgtaacaac tacagatggg atttcacgct tgaatggttg ataatagtct
      961 gattggatgg ttccttctat tttataccat tcatcatttt taaccgttac tccttccggc
     1021 atatgcgtca acaaaccaaa gactcctgaa tctgccacac aatggataat gccgaatcgg
     1081 aacaagaaga tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc ttcatagctg
     1141 atttttttgc caatgaattc gctagggtaa ttataaatta gctccataac ttctaaatag
     1201 ttttcatctg tgatcttgat agtttgctta tccccatact tttctttcaa ctcattcatt
     1261 tggtctaagt agtctgattt gttgaaataa atactggtat ccggctttaa gtattgtgtc
     1321 tgcatctccg gatcccctac tgattcttta cttactggga aattaaatcc tttggcttct
     1381 acgatcgtag tgtcgagact aactgttgga aacaacgtcc ccacgattac tggcaaagct
     1441 agcagtacat aagcgattcc acgttgataa ggttttgaca acccatggtc atgatcgtgc
     1501 tcgtgtgcct tttcttctgc ttttccgttt ttgacccaca aatacagctg aactaaagcc
     1561 aaaagagcag ataaaatcat tgaaagaata gctaagtagc gataatggac attgatatat
     1621 tgatcgagtt tgccagaaac ttgcaagtac atcatcaatt cgaaataccc agataaaatc
     1681 aaaaaccgca gcatattttc cctcctaaat aaataatgtg aatccaataa cgctaagggc
     1741 aatcagtccg atcagcgata aaataaagcg tcctgtaaaa tatcgtttca tcatcagtag
     1801 atttttgata tcgaccattg ggccaaagac caaaaaggca gctacaggtg ctgtgccgaa
     1861 cagacttaac aaagaagatc ctataaacgc atctgcttct gagcaaagag acatcgtaaa
     1921 ggccaataac agcatgatga taatggctaa aatctttgtt gagccgagcg ttagcataat
     1981 tctagttggt aaataagtct gcatcgcagc tgcgattaat ccgccaatga tcaaataacg
     2041 acctgtatca aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt gatggatcag
     2101 ctgatttttt ggttcattct tctcactttg ttcttcttca tggacatgat gatggtgatt
     2161 ttctgctact ttagacgtga ttttttcttt caatacagcg tctttttgaa aataggcaag
     2221 ccagacacca atcacaaggg caacaaaaaa gctgcctatc acccgccagc ttgccatctg
     2281 ccaagaattg ccaaatgcga taaaagtgga aaaaatcacg attggattga tgatcggagc
     2341 agtcagcata aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa attgatgaac
     2401 gatcggtacg atgccgcatt cacatgaagg aaagaaaaat cctaagatcg aaccagtaaa
     2461 aatagagaag atacgatttt caggaagcca tctcttcaca cgttctgggg tcaaaaatac
     2521 ttgtaaggcg cctgagataa gacagcctaa taatacgaaa ggtaatgctt caataacgat
     2581 cgataagaaa atcgtactca tctgcaaaac cgaatttgga ataaaagaaa acatgagttt
     2641 cctccttttt atttctagtt cattcgcttt gcaaatatac gcttttattc aaaaaaaaca
     2701 agttgctctg taaattttta cacaactctt attttttacc aaagcacttc aattgacttt
     2761 ttctgttatt cccattaaat taaggtctca tacatatcac ttctatcata aaagggagaa
     2821 taaaaatgaa acaaaccact aacttgactg aagttcaaga catactgcaa cagtcatccg
     2881 ttgccgtcat ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt cgtccacgac
     2941 tagaggaact tctcacacat tatgatattc cggcacttca tttggatgca tttgaaacac
     3001 cagaagtagc tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt tttcatcaag
     3061 gaaaagaagt ctttcggcaa gcacggttca tcgactttaa aaaaatccgt tatctactag
     3121 atgaactgac agcagaagat gattcccttt cttatgaaga aatcttccga acaaaataat
     3181 cgttgtccaa acagaaaaaa gcgattgaac aagtgaaagt tttgccttca tttgttcaat
     3241 cgctttttat ttttattctg ctgtattttc ggaatgcgca tttgcgtcag aatgcaagaa
     3301 atagatcaag gtttgcaatt cattcgtcaa atcaacattt tggatcaata catctgacgg
     3361 agcattcaat cgagcagcgg taaagttcaa gattcctttg attcctgctt ccgttaactg
     3421 attgactact tcttgtgctc tttcagcggg tatcgttaaa attgctactt cgatttgttg
     3481 gatcttgatt tgttctgaca tatcttccat tggataaacg gggataccgt ctacgatgcg
     3541 cccaacgatt tcctcattca catcaaaagc acaactgata cgtatgctat tactttgatg
     3601 gaatttgtat tttagtaaag cacttcccaa gttccctaca ccgatcagcg caacatttgt
     3661 caattgatcg tcgtttaatg tctttgcaaa aaagtgcata agatcttcta catcataacc
     3721 atacccacgt tttcctaatt cgccaaaata agaaaaatct cgacggatcg ttgcactatc
     3781 tacttgaacg gcttcactta attcagttga agacactttt ttctttccag cattatttaa
     3841 aatccttaaa tagcgataat acaaaggcag tcttttagct gtcgcctttg gaataatatg
     3901 atctttcacg aaatgacctc cacgtattgt ctaattacat ctttgatttc tccatcatag
     3961 cagtatttat aggaaaaaat caattccatt gctcagatat tgtgaaattt tattctttat
     4021 tcatttattt atcatttctg caaatctcgc agtataatgg aatttatggt agactactaa
     4081 ttgaaaaata gttttttatt tgaggagaat tcacaaagta gaaaagagga ttccatgata
     4141 ttattacaag ccaatcagat tgctcgccat tttggtgcag aagtcttatt tgaaaacata
     4201 catttagaaa tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg cgctggaaaa
     4261 tcgactttat taaagatcat tgctggaatt gaagcacctg atgaaggaaa gatcgctaaa
     4321 aataaaacag cgactctcgg atacctcgct caggacacag gacttgattc caatgaaaca
     4381 gtttggaacg agatgctgaa agcttttgaa caagttagac aaatggaaga gcggatgcgc
     4441 gagctagaga ttttcattag cgaaggtacg ccagacacac ctgcttatag ttcttattta
     4501 aaagaatacg accggctgca acacgatttt tcagatcgaa acggctatgg ttatgaaaat
     4561 gaaattcgtt ccgtattgca tgggtttaaa ttcgattctt cattttatga tcagaaaatc
     4621 gatactttat caggcggaca gcgcacacgt ttagctttag ctcgcatgct tttgcaacac
     4681 cctgacattt tgattctaga tgaaccaacg aaccatttag atatcgagac actttcctgg
     4741 cttgagaatt atctgcaaag ctatactggt gcattattga ttgtttccca tgaccgttat
     4801 tttttagata aagtagtcaa tgaagtctat gaattaagca gaagaaaaat gcgccattac
     4861 aaaggtaact attcgaaata tctagatttg aaagcagaac agttagcaag tgaatggaag
     4921 gcttatgaaa agcagcaaac agaaatccat aaattagaag acttcgttgc tcggaacctt
     4981 gttcgcgcct ctaccacaaa acgagcgcaa agccgcagaa agacattaga aaaaatggac
     5041 cggctggatc gtcctcaagg ggatgaaaaa caagcaaact ttctattcag tatagaaaag
     5101 ccttctggaa atgtagtctt gcaaatcgaa gatgccgcta tcggatatga taatgtcacc
     5161 ttatctgaac caatcaatct agatatccgt cgtcaagaag cgattgctct agtaggtccg
     5221 aatggtatcg ggaaatcgac gttgctgaaa tcaatcattc accagatccc ttttatccgt
     5281 ggaaaagaaa cgctagggac aaatgtcacc atcggctatt acgatcaagg tcaagctgat
     5341 ctgcatacaa acaagacgat cctagcagaa ttgtgggatg agcatcctac cactccagaa
     5401 aaagagatcc gaagtgcttt aggtgctttc ttattttctg gcgaagatgt agaaaaacca
     5461 atccctctgc taagtggcgg tgaaaaagca cgggttgctt tagccaagtt agcgatgaac
     5521 aaagaaaatt ttttgatttt ggatgaacca acaaaccatt tagatattga caacaaagaa
     5581 gtattagaaa atgcattgat cgattatgac ggcacactgc tttttgtttc ccatgaccga
     5641 tatttcatca atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg cagcaaagtc
     5701 tatctaggag actatgatta ttatttagag aaaaagcagg aagaagaaga aatcgccgaa
     5761 ttgttggcac aagatgagcc agaatctgct cctgctccca cgaacaaatc tgattattac
     5821 caaagcaaag aacagcaaaa gcttatccgt agccttcaac gaaaaattac tcaggttgaa
     5881 gaagaaatgg cgcggatcga tgaatggatc gatcagttgg aacaagaaat ggtcgcgcca
     5941 gaaaacctgg aagatcacgt aaaactcaat gagctgaacc aagatttaga agctgctaga
     6001 caagatcaag aaacaaaatt gacggaatgg gaagaactaa gcttggaatt agaagaatat
     6061 gaaaattaac gaaaaaacca aaacagaaac cattcatgct gttttggttt ttcttttttt
     6121 attttatcaa acaaaaattc catatttacc tttttcacca ataagaagac caattactag
     6181 tcattctcac taaagcttct aaataatagt aatcacccca aatacaaggt gtatttaccc
     6241 cattattttt gggcttatta taaacagact gtgttaaaaa gccatcttct tcttccgttg
     6301 tagcatatct ttctttcaat tgtcgcataa ttttccaaac agcagactgg tataagttct
     6361 tttctgtttt gtctaataga cctgattcta ttaattctaa taatccacaa accatgatca
     6421 ccatactaga tgtatctttt tcttctctgc tagaatcatc aaagatcaaa tcccaataac
     6481 aaatggtatc ttctggcaac cgattcataa aataattggc taatattttg cttaaatcaa
     6541 aatatttttg ttctttcaaa taacgataac ttaatgcaaa accgtaaatc ccccatgctt
     6601 ggcctctagc ccaacaggaa ttatcactat aaccttgatg cgtacttcca tatcttggtt
     6661 ttccatcttt gacatccata taaaatgtat gataagttga accatcttct cttactaaat
     6721 aatctgctgc attttgaata tgagcctgag cagctttttt atatttttga tttccagttt
     6781 cttcactcgc ccaatacaat aatggaagat tcatgcaaca atccatgatc attctacctt
     6841 gttgtgaagg atcactagga tctccccaag cttgtataac tcctgccttc tcccaaaaac
     6901 gctgaagtaa tgtttctgca gcatcaattg aagcttcctt catttttcga tttcccgtta
     6961 ttttataatc tgctacggtt gataaagaat acaaaaaacc aagatcgtga tgattagtga
     7021 taatttgaca gtcaatcctg tggataaaat ctatatctgc tttttcagct gtttgttgaa
     7081 ataaaggatc acccgtcttc tcaaataaca accaattaat tcctgtccaa aatccttctg
     7141 tccaaccaat attgtctctt aatggataac gtccttttat aggacactcg cttgggaatt
     7201 gatactgact gttactgata cgtttgatta acctctccgt gtgtctgcga caacgctcga
     7261 tctcattatt ccattctttt tccattaatt ctttataaca ttcatttatc catttttctg
     7321 gatgtattat ctcttcaaac atcactttat caagcatttc cccaccagcc tttagcataa
     7381 ttttagtctt tttttattgt ttttattttg gatagttagc ccggttgtcc catcaaaatc
     7441 aattttggga cgattgttcc atacttctaa actataatcc cgatcttgat gagcaaccac
     7501 tcctgttccg agccaatggg ttccttttcc aaatgtaccc tctaatccag gaataaaagt
     7561 gttttcccat gtcatcaagt ttgtattggg caaacccata atagctgtgg attttcgttt
     7621 aatagtatcg tcacttaaac agataatacc tgaaaagcca gcttcactca ccctatagtt
     7681 ttccttgtct tcttctatga ttattccttc tttctcagga gaacattcaa tagcaaaccc
     7741 tgtctcatat agaacatatt ctgtatctaa ttctactttg tgtacgcgaa tctgccagcc
     7801 ttcaaaagga ataagccaag ttctgatttt tgcctgtaaa gtaggttccc atgtagaggc
     7861 aagtacctct ttcttacata caatgtctcg tacattattt cgaacgatga actgtccata
     7921 ggttttgatg ctctcattct taaaactggt cgtgatatat gtgtccgctc gctgaatccc
     7981 tagcattgaa tccattgcca tttctgcttt caattgattc gctcgcggta tcgaaaaacc
     8041 aaattgacta gaataagcaa attttaaata tttttcattg gtcaatgtat ggaaatttgg
     8101 ccctaattgt cccgcattca aaaaaaatgt atgtccatta tcatgttgta tgagcaaatt
     8161 ggcttctgaa agaagtttag ttccttcacg aataggcata ggcatttctt tagccgtcca
     8221 atactcatgg tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc ataatggaga
     8281 taacgtacta ttatagggtt cagtcaatgt caattgaggg taggtataac caatattcag
     8341 gatacctttt tcatcaaata tttctttttg catccaccat cttatattcc gattgactaa
     8401 ccccttgatt tcacctaatt taaaaggcaa aagatcggcc cacaacatag ctgaccaaaa
     8461 gcttgaaact gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg caccatcttc
     8521 cgcaaaaaaa tgaatatagt ctctagaaaa atcaatagct cggttcttat atatctggct
     8581 aatgggcttt tctggttcta gtacgctata aatcaaacca taataatgaa atgcaaaagg
     8641 attataataa tcttggcgac ctcttgaact gtctcgatac cagccatttt gaagataaca
     8701 attttctata ttatcaaatg cttctttcag tgctgattct ttaactggat aaccaaattt
     8761 ttggataatt gttccgacaa ttatcttaaa aaattgccaa ttaccatctg cacattcata
     8821 ttcaaaaatt tgcgagaacc aatgaataat ctggcttttt tctcctgtat ctaatttttt
     8881 ccataataat tgttcatgat gatataacgc taataacatt gcgggcattt ctaccatacg
     8941 ctggtctctt tcatgtattt ctccccaata atcgggatgg gaaggatctg ttccagcttt
     9001 tattcctgat agaattaatt tcaaccatcg ttcatctaat tggtcaattg cgggccccgc
     9061 tccccataaa acccgagaga atccttccat tcctgcaatt ttatttccat atcctacccc
     9121 ttgatgtata taatcaattt gtgcgccact tgctcgataa aacggctgga gtggttgatt
     9181 tatcttttta aataagtcaa agtaatcctg tctggtcctt aatagtggta ctggatgttt
     9241 cattgtattt tctcctcttt ttactaccaa atagctaatt ccgactgttc taatcgaagt
     9301 aagccttcaa taaaaaaata atcagaatag atcagtggca cattataagt cagttctcta
     9361 tggtatgctt ctgctccgcc tccaatgatc ccatcttcat ccatattcca atttgcatag
     9421 tgcttttctg tcttttggag tatattgata gcagcttcat gatacatttt tgatcgtaca
     9481 gaggaaacat atcctgctaa ttccagaaga ccgcatgcag cgcatactcc agcactggta
     9541 tcaatattcg aatcttttac agcaaaatca atgagaggaa tataaccagt tttttcgatt
     9601 ttctgaataa aatttactgc tatcttttct gctatagcta gatagtacgg attttttgtg
     9661 taacgataac ttaacgcgaa tccatataaa gcccatgcat ttcctctgct ccaacaagaa
     9721 ttctcattgt atccttgccc gccaagtaac cgaatatatt tccctgattc tgcatcaaat
     9781 tctgcgatat gcccggttga tccatctttc ctcactagat acttagctgt tgtatctgca
     9841 tgctgccgag caattttaga gaacctcgga tcaccagttt ccctcgaagc ccaatacagt
     9901 aatggtaaat tcatcatact atcaataatc atccaaccag gtttatggtc attccatgct
     9961 actaaatagt tccctgaaat attatagcgt ccagcaagta ccgtagctgc atgtaaccct
    10021 agttgtcgac tatgtttgtt ttgagactgt tgataatgtg caatagccga tggtaagaat
    10081 aaaaaaccta catcatgatg aacgtctgaa aaatggtaca gggcagattc taataattgt
    10141 tcttgttgta ttgctgcctc tttgaacttt tctcgttttg tcttgtgaaa gagctgccat
    10201 aaaataccac tccaaaaacc attggtccac cagtcgattc ctctgtcctt tattacgtct
    10261 gtatattttc cgttgtaaga aatatatgga attttattcc ccattctgtc taattccgca
    10321 tctatctttt cagaagctac attcaaaatt tttcttgacc attccttcgt ctgttcattc
    10381 atggatatcc cgccattctt ttaattttta tgaatgtgga gttgctccta gaacgaacta
    10441 gatggttaac attcgttatt ttttttactt atttgcagct ccaatgtcat tctccttaaa
    10501 tttagactgc gcccacacat aaatcaagac cattgggatc actgaagttg ctaaaattag
    10561 cataattgcg ccataattaa ttggctcacc tgtactagaa accgtcatat ttttgataat
    10621 taagggaacc gtatatttat ctttatctga aagcatgact aatggaaata gatattgatt
    10681 ccaacttccc ataaagataa gtaaaccact agtaataatc gtaggtgata aaatcggtac
    10741 agcgattttc gtaaaaatca cccactcatt ggcaccatcg attctggcag cctccatcag
    10801 tgctgtagga aattgactgc tatattgtcg catcataaac actgaagttg tactggcaat
    10861 cgaagggatg atcacacctg cataagtatt cgataatcct aaacgactca taatgtaaaa
    10921 taaaggaatc aatgtggtaa aaaaaggcac catcctagat agcataactg tattgaacaa
    10981 cagtttattc cccataaaac gatactttgc taatgcataa cccgccattg tagaactggc
    11041 tgtagctaaa actgtactga ttacagcaat aacaatcgaa ttccaaataa tcaagggata
    11101 attatatctc gccatgatgg cttgccagtt ttttaatagc atatccccaa acttgaaatt
    11161 gattccgcca cttaaaattt cttgattact ttgcgttgaa gctacaatca tatataaata
    11221 tggcatcaaa aatattaagg caccaaacac aataatgacg tacgtaaaaa ttctgctttg
    11281 tatcttttgt ttagtcattt ttatcacctc cgaatttaaa ttgaatagcc gataatataa
    11341 cagatacaaa caccatcgtc caggcaatag cagaggcttt tccaaaatca accatttcaa
    11401 aacttgtttt atataaattg actcctacga ccaaagctga attttctggt ccattggaag
    11461 tcccaaagaa tatatttggg atattgaaat tacccaagcc attgatagtg gctaaaactg
    11521 tggccattaa aatgattggc tttagcatgg ggatcgttaa taaaaagaat tgccgaaatt
    11581 tcgatgctcc atctatttca gcactttcgt atatctcttt agaaatccct tgaagaccag
    11641 ctaaaaataa aatagtgtta tatcctacac taagccaaat catcgtgatg atcattgata
    11701 ttcgtatcca aaaaggattt gttaaccact gtacaccgtt aattcctagc gcattcaaaa
    11761 atccattgaa taacccagtc ggattgaaaa aagtcatgaa tacggaagct acagccaccg
    11821 ttgatgtaat ggttggaata taatagacag ttctaaaaaa tccttttcct tttaactgtg
    11881 ctgaattcaa taaaacagca aagtaaatag ctaatagcat cgttattgga attgatcctg
    11941 ccatgataat caaaacattc gtgtaagatt taataaacaa tggatctcgg aagatagcta
    12001 gataattgtc aaatccaaca aattcaaatg agtagccaat tttcataaaa ctaaaagcaa
    12061 acgagataat gatgggaatc aaaatcgtca caataaagaa aataacaaaa ggtgtgacca
    12121 atccataagc acttttttta tgagacgtat ctgttgcttt tttcttgctg ttctttattg
    12181 tcttgcttgg taacgaagat tgcatcatcg ttaagtcctc ctaatatttt ttaatcacca
    12241 gttaatttta cgccgtactt atgagcaaaa ttttctgctt cttttagata tgaattttca
    12301 aaatcacctg tttgccaata gttatatgca gcggtagtca aataagacgt agcatctgaa
    12361 taagtggctg gtaaaacagt atttgatgta ttttcattga ttttattcaa tagttcatag
    12421 tatttttgtc ctccttagta gtcaaacttt ttctcagctg cttctgtttg ataggccaat
    12481 gagttagatg tagccacgcc cagctccagt gcgttggcta agcattcttc atctgtcagc
    12541 ataaatgtaa gaaattgttg tgccacttgt ggatttttgg catctttagg gacataaaaa
    12601 cttgatcctc ctgatactgc accttttcct ggattttctt ttgaaaaagg aatcagtgga
    12661 gccatgcgcc atttgtcaga tgcatcagga aaattcaaag acatattcgt agcataccag
    12721 ccgccagcaa caaacattgc agattcttga aatgctttct cagcatcttg tgcaccgtag
    12781 aaatgtacaa tatctgagtc gatcatttct ttgattaact ttgcagcatg tttcgcttct
    12841 ttagtattca gacggatatt tccttcttca tcaagcaaag attgattctg ctgggctagc
    12901 atgttgataa acaattcttg gtccccagat tctggcaagg caattccaaa actaccagtt
    12961 ttttctttta atgcttttgc cacctggata tattcttccc aagaactgat ttcttcaaaa
    13021 tctacaccta tttcagcaaa tttttttggt tgataataac aaagtgctgg ggtaaaatca
    13081 ctagcccaag gaataatcgt ttcattactc gtttgttgac gcaaaatatt caatttttga
    13141 tcataaaact gatcgccata tttgtcataa aatccatatt catcagcact ataaaaagca
    13201 tcttgaaatt tttccagcac acctgctgct gaagtgtctg ccatggagat aatatccggc
    13261 atatcttcgt gtgttaccaa ttttggggtg atcatttgta ctgttgctgt tcccgaagct
    13321 acattcacaa tttttaagtc aatatttgct ccagttttgc ggttatagat ttttgcagca
    13381 ttttcaacat agtttgtcag tttgccgttt gcgacatata cttctaatgt agtggctttt
    13441 tttatcattg gatctgcgat gtttgctgtt acttcttcgt tagtttttcc acatgcactt
    13501 aagctgaata atagggtaat gtttagcaaa gctatcacca atttgtatct ttttttcatg
    13561 tctatccctt ctttcttttc aagttcttat ataaaacgct ttcacttatg tatattaacg
    13621 gaattagtaa cacaaatatt tatataaaaa aatattttac ttgtcgtttt ggcttttcat
    13681 ttttgttata atagtcatta attataaata gaaagctttt tatataaaaa gagaaaggaa
    13741 agtggttctt ttgttaacaa acaaccaatc ctccaatctt gtcaaaaaaa cactaaccta
    13801 ttccatagat acccatcttc atttatctgc tgctccctat tttcgactac taataaatga
    13861 aagcgctaat ttatatgctg aatttgcaca taaatctata gaactagata atccttccct
    13921 tttattcatt gataaacagc aagaagtcca aatcttttct actcaaaagg aaacacaaac
    13981 ttttatcatt atccagtttt attttgatga gcaattatta gcacatatta cgggacaatc
    14041 tttttctata gatcaagaaa
//
LOCUS       DAZKNO010000065        13970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000065 DAZKNO010000000
VERSION     DAZKNO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.066"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..860
                     /locus_tag="ACG8MY_001446"
     CDS             12..860
                     /locus_tag="ACG8MY_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0430642.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1031..1372)
                     /locus_tag="ACG8MY_001447"
     CDS             complement(1031..1372)
                     /locus_tag="ACG8MY_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFQ0430643.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1458..2165
                     /locus_tag="ACG8MY_001448"
     CDS             1458..2165
                     /locus_tag="ACG8MY_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0430644.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2152..2475
                     /locus_tag="ACG8MY_001449"
     CDS             2152..2475
                     /locus_tag="ACG8MY_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0430645.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2564..3601)
                     /locus_tag="ACG8MY_001450"
     CDS             complement(2564..3601)
                     /locus_tag="ACG8MY_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0430646.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4048..5031)
                     /locus_tag="ACG8MY_001451"
     CDS             complement(4048..5031)
                     /locus_tag="ACG8MY_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0430647.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(5447..6217)
                     /locus_tag="ACG8MY_001452"
     CDS             complement(5447..6217)
                     /locus_tag="ACG8MY_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0430648.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6210..7145)
                     /locus_tag="ACG8MY_001453"
     CDS             complement(6210..7145)
                     /locus_tag="ACG8MY_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430649.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7739..9388)
                     /locus_tag="ACG8MY_001454"
     CDS             complement(7739..9388)
                     /locus_tag="ACG8MY_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0430650.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(9583..10479)
                     /locus_tag="ACG8MY_001455"
     CDS             complement(9583..10479)
                     /locus_tag="ACG8MY_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0430651.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(10472..11152)
                     /locus_tag="ACG8MY_001456"
     CDS             complement(10472..11152)
                     /locus_tag="ACG8MY_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0430652.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(11153..11935)
                     /locus_tag="ACG8MY_001457"
     CDS             complement(11153..11935)
                     /locus_tag="ACG8MY_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0430653.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(11928..12845)
                     /locus_tag="ACG8MY_001458"
     CDS             complement(11928..12845)
                     /locus_tag="ACG8MY_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0430654.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(12848..13132)
                     /locus_tag="ACG8MY_001459"
     CDS             complement(12848..13132)
                     /locus_tag="ACG8MY_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430655.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
BASE COUNT         4118 a         2997 c         2450 g         4405 t
ORIGIN      
        1 aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg gaacgcggtt
       61 acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag tacttgtccg
      121 caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa aagaccaaca
      181 tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg gctgctgtct
      241 tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc gaactctcgt
      301 cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag attcttatag
      361 aacatccgca ctttatcgaa cgcttcaaag ttaagatgcc atttcatgca ataaattatt
      421 cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa aacaaaaaca
      481 ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca gttgaatcgc
      541 ttattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg attgctactc
      601 tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata gaggactgtt
      661 attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg caaatgttct
      721 cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc tactctataa
      781 aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa aaaatagata
      841 aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa aaatagcaag taaacgataa
      901 caaccatcct aaatccacaa gaaatacagc agaccttgct ttaaaaacac gaatcatgca
      961 aaggatgaag taaagaagac ttaacgcgta cccaatatta gtcatttgac tattaggtac
     1021 gcgttttgtt ttataagtca tgacaaattt ccttcgttgc tggatagatt ttccggcatt
     1081 gttgatagat tttatgatac ttttctacat tttgtaagag aggttccgct gcatctgtat
     1141 attggataaa attctgacaa caactttcag gtgtgtcgaa ccaccctaac cctagtgcag
     1201 caatcattgt tgcgcctaat gcaggtcctt cctccatact taaacaatag accgttgcat
     1261 cgaaaatatc agcttggatt tgtagccatg caagattttt cgcgcctcct cccaccgaaa
     1321 caatacgtgt aaatctgcga cccgctagtt ctttacgttt caatcccacc attgacaaat
     1381 gctcattaaa aatttagaat attcacaagt ttaacgacaa ttatgaaaat ttatgtcgtg
     1441 ctaaaggagt tttgttattg gatgaaaagt tagatataaa aactgcgatc aaagacacgt
     1501 tacctaccgt tttcggttat atcggtattg gacttgcatt tggtatcgtt gggaaagctg
     1561 ccggatttca tccactagtc gtcacgttga tgtccctgct ggtctatgct ggttctgccc
     1621 aatttatcac agtcagcatg cttgctagtc acagcccatt gctttccatc gttttctcga
     1681 cctttctagt caattcccga atgattctga tgagtatgac gattgctcct tatttcaaga
     1741 aaaatcggct gcttcaaaat ctgctgattg gcacattgct gacagatgaa agtttcgcac
     1801 tgggaatgaa taaattgaac tatactggac aaaagttaaa ctttcgttgg atgaatacag
     1861 ctaattggat ttcttatttg acgtgggtgg gttcttctct tgttggcgca cttttaggga
     1921 attttattac tgatccgaaa aagtttggtt tggatttcgc catcgtcgct atgtttatcg
     1981 ggttacttta tcttcaagtc atttctgata gaaacacctc taaacgatta cagttgatcc
     2041 tgattggtct cactttgatt ctggtatatg tcggactgat ttttatccca agcaacttag
     2101 tgattgtagt tgtaacactg attgggtgcg gattgggggt gtggatcaaa catgccttct
     2161 tttgagttta cattgctgac tattttaggt tgtactttag caacttggct ttctagggtc
     2221 ctaccatttg tcttactgaa aaaattcgat ttaccgcaac cgttgcttga gtatttaagt
     2281 tttgtcccga tcgtcattat gtctgcttta tggttcagca gtctatttac ccaaaatatc
     2341 ggacatctgc cgcagatcaa tctcgaaaat gctttggctt cagttcctac acttttagct
     2401 gctattcttt caaaaagctt attggtgatc gtcttggcag gaattctttc cctttcgttg
     2461 attcgcttat tataaataaa aaaacaagag cttatggcaa attactgtca tgcgctcttg
     2521 tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct tgcttatccg ttgaacggac
     2581 cactaacaac gacttttcct aacatatgtc ctgtttctac gtctttggtt gcttgtttca
     2641 agttcttcgc attcatccca tctgaataga ccttgctcaa tgtactgcgg ataatccctt
     2701 catttgctaa agcagcaatg tgcgctaaag cttctccttg cgtttcgatt tcatagttat
     2761 agtctgtttt agcgaacata tactcccagt cgaagctgat agattggttt ttccaaacac
     2821 ctatatctaa cggctctttg atccctacga tcgttcccac atgtccaaat ggtcggacaa
     2881 ggtgcttgat ttgatcaaag tagcgggtaa tatcgaataa tacagcgaca tactctactt
     2941 gatcgattcc taattcacga aaacttttac ttaagtcgtg atgataatcg attggatagt
     3001 ctacacctgt ttttttcagc cattcaaaat tcttcggact tgcagtcgcg tagacttcta
     3061 ggccagccca atgcgctagt tgattcaaaa tcgaaccgac gcccccggat ccgttgatca
     3121 ctaagatttt tttcccgcga ttggcatttt cttctggagt tagaccaaat ttttcaaata
     3181 acaattcata agcggtcaat gaggtcaacg gcaaggctgc agcttcttca tctgaaagat
     3241 tttttggtgc aagagcgaca atacgttcgt caactagttg gtattcttga ttgcttcctg
     3301 ctcttgttgt tgtacctgcg taaaaaacgc gatcgccgac agaaaatttt tgcacttcgt
     3361 cacctacagc aacaactttc ccgactccat caaaacctaa gatacgtaaa gcatttctta
     3421 tcccgttatc ttggcgaagt ttcgtatcga caggattaac agatactgcg ttgactttta
     3481 ccagaagatc ccgtgaactg ggtacaggta tctgttcttt ttcatcaata aagctttttg
     3541 gatcatcgat cggcaaccct tcataaaaac cgactacctt cattaattcc ttttctgaca
     3601 tacgaattcc tccttagctt catcaagttg gagcatccat acagtgttca tttcaatata
     3661 tatagtatgc tatttgttcc acctttttta ttcatcataa ttattctctt acttaaagta
     3721 aactatttgc acttttttgg ttaaaaaaga ggttgcgaca agacttttgt cacaacctct
     3781 tataaacggt gttctcctac caactcttcg gtcacaagtc aaaatattca tcaaacatga
     3841 gaagctgttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc
     3901 tgatataaac ggtgttcaat caactgaccc tcggtattag ctcaaaaaac gacaaaatat
     3961 gaagagctat ttttgtttgg ctgttcttat tacttgatac aggacagctt tttcacgacc
     4021 tctaattcac ggtgttcaag caagaaatca ttgttcactg aaaagcgttg gatgttcttt
     4081 ttcaaaggtc tctaagatct ctcgtacttc atcagtagat ttcgtattca tcaactgaac
     4141 tcgcaagtca tttgctcctg gaaatccttt gatatagatt ttaaagaaac gatgtagtcc
     4201 tgtgatggaa cgcgggatga tttctgaata atgatcttgc agatctagtt gcagacgaag
     4261 tagtccgatc agttcttttt ctgtatgttc cctcgggtct ttttcaaatg cataaggatt
     4321 cttgaaaatc cctcggccaa tcatgatgcc atcaacgcca tatttctcgg ccaattcttc
     4381 tcctgtctgg cgatcgggga tatctccatt gatcgtgatt agtgtttgtg gagcgatttg
     4441 gtcacgtaaa gcaatgattt taggaatgac ttcccaatgt gcatctactt tactcatctc
     4501 tttacgcgta cgcaaatgga cagaaagatt ggctatatct tgatgaagta ggtgactgat
     4561 ccattcttcc atctcagaca tttccgtata gccgatacgc gtcttgacgc tgacaggcaa
     4621 accgccagct ttagctgctt caatcaattg tgccgctact tctggacgaa ggatcaagcc
     4681 actgcctttt ccacgttctg ctacattagg gactgggcag cccatgttga tatcgattcc
     4741 ttggaagccc atttctgcca tagcaatgct catttctcta aaaaattctg gcttgtctcc
     4801 ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaat gttaatcttc ctcgtacact
     4861 gtcttttcca tctggatgac agaaactgtc tgagtttgta aactcagtaa agaagacatc
     4921 cggtgctgct gcatgcttta cgacatggcg aaatacaaca tctgttacat cttccattgg
     4981 tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa ttagtagtca tttatttgtt
     5041 actctccatt ctatcaagca gtgaagtcgc aatctttctc aaaaacattg tcattatact
     5101 aggaaacaaa aaaagaagca aagtgaacag cttaaaagat gtttctgatt gagcaaaaaa
     5161 aagactgtga caaaaatctt atcacagtcc attttccgga ataaatggtg ttcaaaagct
     5221 tgcatttgcg gtaataagtc caaccaaacg aaaaatagga ggagctattt ttgtttggct
     5281 gttcttatta cttgatgcag aacagctttt tcacaacctc tccactaaac ggtgttcaat
     5341 cagctgaccc tcggtattcg ctcaaaaaac aacaaaatat gaggaactat tttcccgttt
     5401 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tcttttctaa cttctcaatc
     5461 ctcttccttt attgatcaaa taccaagtaa cggcgtacaa aacaacgatg aataacacaa
     5521 gtacgatcat cgatgttgct actggcacat cgatcgttcc aaggaatccg taacggaaac
     5581 cggagatcat ataaacgatc ggattgactt ttgaaacagc ttgccaaaag ggcggtaaca
     5641 tagagatggc atagaaaacg ccacctagat aagttaacgg ctgcaagaca aatgtaggca
     5701 cgatcgatac atcatcataa gactgagcaa aaatcccgtt gagcagtcct gctaatgaaa
     5761 acaagattgc cgtcatcaat aatgtaatga tgaccatgaa ccatgaatac acatgaagtg
     5821 gtacaaagaa caaggaaatg atcgttacga gagagccgac gagaatactg cgacccacac
     5881 caccaatcac aaagccccaa ataatcacat gcgtaggaac aggtgctacc aagatttctt
     5941 cgatattttt ctgaaatttt tgagagaaga aggacgaaga aacattggca tacgaactag
     6001 tgatagcaga catcataatc aaaccaggaa cgatgaattc catatatgaa aaaccaccca
     6061 tgtcaccgat ccgcccaccg atcatttttc cgaaaataac aaaatacaag gatgtcgtga
     6121 tcactggcgg aaccagtgtt tggacccata tacgcaaata gcggttcgtt tccttgatag
     6181 ctaaactttt taatgcagta aaataaagat taaacatttt tctcctccgt atgattattt
     6241 tcttctgtga ttttcaagaa cagttcttct aaacgattcg acttattcct catagaaaga
     6301 actttgattc cttgtcttgt aagctgatcg aagattccgt tcaccccttg atttcgttca
     6361 acttcgacag cgattgtctg ctcatcttca aatgtacaag catatccttc aatcacaggt
     6421 ttggtatcat atggcgctaa atcaaagata aatgtttcaa attgcagttt tgacagaagc
     6481 tctttcatac ttgtattctc gatcagttca cccgattgga tgattccgat gttgcgacaa
     6541 agcatttcag cttcttctag ataatgcgtc gtcaaaatga tcgttgtacc attttcgttc
     6601 aattctcgta gaaactccca catctcgcgt ctcaactcga tgtcaactcc tgctgttggt
     6661 tcatccaaga tcagcaaacg tggttcatgc atcaatgccc tagcaatcat cagtcttcgc
     6721 ttcattcccc cggaaagcat acgtgcacgg acatttctct tttcccaaag attggactgc
     6781 ttcaaatatt tttcgctgcg tttcatcgct tctttgcgag agacaccgta gtagcctgct
     6841 tgattcacga cgatttgctg gaccgtttca aaagggttaa aattgaactc ttgtggaacc
     6901 aatccaatct gttgctttgc tcgaactaga tccttgtcca gatcataacc gaagacattc
     6961 acttttccag atgttttatt gactaaagaa gtaataatcc cgatcgttgt agacttccca
     7021 gctccatttg gccctaatag cgcatagaaa tctccttctt tcaccgaaag atcgattccc
     7081 cgcaatgctt cgaccccact tccatagatc tttttcaaat tatttatttc aagcgcattt
     7141 gccatttatt tcctatctcc ttatagacat gattcttaat tgtatcatgg aatacagcag
     7201 atgaaaaaac aacttggctt ccacacaaaa acacacctgc taacaaagag gagatgtgtt
     7261 ttctagttca tgatcatctt cgatagaata actatctaaa caactctgga acttttaaaa
     7321 tcataaaagt accgacagca actactacga tgacatatag aagatcctta taactgaggt
     7381 tcttgtcttt tttattcatg ttaagctttc ctccgtcctt ttatcgaaag cttttttctt
     7441 ttattttaac cgaaataaat aaaatggaaa tagttatttt caatttctgt tttcgctgtt
     7501 tgttttacgg agaactttgc catggaacgt gcgtctcgtc gctgatgcta agttttttcg
     7561 ataaaagctg ctagaaggaa gacagcgtgg tttattttgt atcgtctttt gctaaatctt
     7621 ccaagatcat ctggacagca gactcgttcc cattgaattc tgccactttt ttggcatcaa
     7681 tgtacttctt ttgataatcg tctttttgga cccacgcatc atgcttcgtt tgataaagtt
     7741 aatcaaacgg ataccactga cgctgttcat cgaacaacaa actttctttt tgggcctgtt
     7801 caactatgga tttaggataa ttcaaaactt tcagcaaggc aatcgcattt cgggtccttg
     7861 aaggtccttc ttttaatttg aaatcaaaac tgattccttt tccttctgtt acttgttctt
     7921 caaagtggac attctcacaa taattcttta aaatctctgt caattcgata tcatgtgtgg
     7981 cgacaaatgc taaagatgga tactccgcaa gccaccgaac gactgaagcg gaagaagcaa
     8041 tccgttcgat cgtatttgtt ccttttaaaa tctcatctac gaagcagtag cagcgttctt
     8101 tggaagccac ttggtccagc aaacgtttga tcgatttgat ttctgaaaca aaataactgt
     8161 cgccttcaaa taaatcatct tcgattgcca tggaagtcaa cacatgtcca ggttgcaggg
     8221 taaactgttc tgcaatcgct gtttggatcg tctgtgctag aatacagctg atggctatac
     8281 ttttgacata cgtagatttt cctgatgcat tggaacccgt gactaatgta ttcttctgcc
     8341 agttgactgg attaactacc gcttctttta acaatggatg atagatatct ttcgctgtca
     8401 caccgccctc agtaaactct ggtcggcaag tgatcggcat ataggtgcga taattcaaaa
     8461 cggctgctgc aacttccaat tctcccagca attcccacag cttgatggct tcagttgaat
     8521 gcttttctaa tttagtgaga acgaaatgat aggagataaa tggcagcatg aacatgatgt
     8581 tgacatattc aaaaatcaat tctgcttcac tcccgctctt cattcgaaat gaaaaaccta
     8641 atttagggat gtctcttaat ggtttagttt gctgtttcag ctctttttga agtggtgtcg
     8701 tcatttttgc taactgattg gcggctgaaa tcgttgaaac caaataactc atgctattca
     8761 gctctgtttc tagtgtttgt ttcttcacta gataatagac cgtattgaat actaagccac
     8821 cgattgctag atagattcct agtacttgcc ctaaaagaag caaacctaca cccaccagcg
     8881 gaagcaagcc aagaaaaata aataaaccta cgcgtccaat ctttttactt tttccatccg
     8941 ctagatattg ttttgcaaaa ttatgatctt tttttcctaa acgagcgaat tgatattgga
     9001 ttctttctct cagttgagga ttttcctgat aaaaagcaat tagtttttct aactgataat
     9061 cttctccaaa atcaaagcta cgtaaacgct gatacaacgc ttctgaacct acactagaat
     9121 aggtactgtt gatcccttca aatacttcga acatatccaa gtcataccaa gtaagatcgt
     9181 cgatttcact atcccatgaa cggaattttt tctccgtgag ccacgcatcc ttcaaacttt
     9241 cttctttatc aaatctcggt tgataaggaa tttttcccca attactacga actgtttcct
     9301 ttactttcaa acgtgaaaaa atgtctaaaa caactaataa aataacaact gcaataataa
     9361 ttaaaacgat aaattctctt tggcccatat ccctctccgt tccttgattt gttagtttta
     9421 gtatagcact gttgcgctat ttgaaaaggg ggtacaaaaa taactgtctt cgttacctaa
     9481 ttcgtatcaa tcacaccaaa ataaaaacac cttccaaaaa agaagatgtc tttaaaacga
     9541 gatacaattt tttgtcattc acttcaggta aattagagcc aattaaaccc tccgcaactt
     9601 gacggagatg gagaagatgc ccccgtctaa tctagcggtc atctctcctc ccattccttc
     9661 caccaatttt tttgacaggt aaagcccgat tccgctagat tccgtatttg tgcggctggt
     9721 attttcggta taaaaacgct ggaagacttt ttcaacagag gtttgcgctc ctggtttgat
     9781 aggattggcc actgttaatc gaatgaatga atcctctttt gaaaatgtca cctttgcttg
     9841 tccatctgca taacgcaata aattctgtat gatattttgc agcactcgca gcaacatgag
     9901 cggatctgct aatacagttg tatttatcga gtgttcagcg aacgctacct cgatattttt
     9961 ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc acctctttag aaaaagaaag
    10021 cgtatggaga tccatttctt tttgcttcgt ctctattcga gcaagatcat aaaactgatc
    10081 cgtcatactg agtaatcttg caacagattg tttgatgatc gttaaatact tctcccgttt
    10141 ctccgtatcc tctacctgtt ccagtaattg tacataaccg ttaatagcag tcaaaggagt
    10201 tctgagatca tgagaaaggc cgacgatcga tagtctgatg ttttcttcca tttcttgaaa
    10261 tgcttgtcct ttttgttcat tttcatccac ccatgtattc agtgcatcta ctaggtccat
    10321 tagattcttt tctcgaaatt cgactgaaag acgaccacca tattcttgtc gttcagaaag
    10381 agtggcgatt ttcttagtta atctcttgat ttccctttgc aggaagaaaa taaaacttcc
    10441 cataaagata acaacgagaa gcaacagcca cttaatcaaa tctaaacccc actccccaca
    10501 ccgtatgaat atacacagca tgtgcctttg ctagtttccc tcgaagattg gaaatatgca
    10561 tattcactgt tttctcacta tcaaaatatg gttcttgcca aactgtttca taaagatttg
    10621 ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt cagcagatcg aattctcttc
    10681 ctgtcaaatg gacagccaca ccattcacat ttacatccct cgactctttg tccagtaaga
    10741 tttccttgta atagatgcgt tgcgacagat cttttacttg gacagaagtc tgatgacgaa
    10801 gttggatgcc gattcgtaac aacagttctt cgatatcaaa tggtttcgtc acatagtcat
    10861 ccgctcctgc tgccaacaag gaaactttat ctctttgatc gctttttgct gaaagaacga
    10921 tgatcggaac attcgatgtc tgtcggattt ctgctaacaa tgcttcacct gaaagccctg
    10981 gaagcatgag atctagaagg atcaaatcga caggatatgt gcttaacaat aattttcctt
    11041 ccgtacctga ataagcatca agaagtttgt atctctgttc aagaacctcc tccagtaaag
    11101 aatgaacagc ttcatcgtct tcgataatca aaatagtttc cagcaggaac acctacttaa
    11161 tgtcagattt ttggaaaata aacattccta gagctgatga taccacgatt gttactagac
    11221 tgaccagaat cattttcggt gcttgcgaat aagttgcttg ttgcgcaaag tccgtataca
    11281 tgagccacgt ttccgtaaac cactccgtta gaaattccaa gcctttgatt tttgcgaaaa
    11341 caccgggaac gaaggccgtt cccatgatat aaagaaaaga aagaagcaaa ctgacactgg
    11401 tacttctagt gatgaatgtc aaagccactg gaacagataa tgtcgctaat atcagcagaa
    11461 gcaatacagg aaatactttt agaacataat ttccatagtt tttgattccc tcaacttcac
    11521 caaatccgaa agcaagtgtg taagcaatca tggctagccc cgtaatcaaa accacgccaa
    11581 gtgttgtgat caaaagcgta acgagaagtt tcgacacata ataagtcatt cgattccgtc
    11641 ctaaagataa cgtattgcga atcgtaccgt tttgaaattc ttccccaata taaaagcacg
    11701 caaatgctgc gaaagccaga agaatccatg cagccatttt ttccaccatt aaaatccctg
    11761 ttgcaccgtt gatgactgta tccgttgctt ggtttaattc attgccgtat tttgtatgca
    11821 tataccattc gttccaagaa taaaaaagcg gaagtacgac catcagtcct agtgagaccc
    11881 acattttacg gctcttccat acttttattt tttcagcagt taatagatta aacatgtgga
    11941 ttgcctccag ttaattgtaa gaaataatct tccagttttt gcccaactag gtggatacca
    12001 tccacaggaa cttttgcctc taccaatgct tgattgatgg ctgctacttg gtcaattcct
    12061 tcaaaaagct ggataacacg gctagattgg acaaaataat ccctataacc aagagagtcg
    12121 agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt ttataaattg acgagattct
    12181 tgtgccaatt cttctttcga taattctttg atcaagtgtc cttcatgcaa aataccgtag
    12241 tgagtggcga tctgtgacaa ttcatctagc aagtggctag aaagcaagac ggtgatgccc
    12301 cgttctttta ccaaacgttg gatgatttct cgcatttctg ccatccctgc tggatccatc
    12361 ccattcgtcg gttcatccaa aatcaaaaat tctggattcg tgatcagtgc atttgcaata
    12421 gctaaacgct ggcgcatacc taaagagaaa ttctttgctt tctttttccc tgtattttcc
    12481 aatcgcatca ttttcagcag gtcttcgatt tctctatccg acacaccgcc gttcgctgct
    12541 tggatacgta ggttatctct tgcagttaat tctggataaa gagcaggggt ttcaatcgat
    12601 tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc caaataattc gatcgaacct
    12661 tcatcaatcg agatcaaacc cataatagtt ctcataaagg tagacttacc cgctccattc
    12721 tccccaatca aaccatagat cattccacgt ttgatttcga tcgatacttt atctaatgct
    12781 ttggcagcac cgtatttttt ggttatacca gttgctttca agaccgtttc atttccaaaa
    12841 tccatactta tccttctttc ttattgataa gtgaagtata gttgacgggt ataaagaaag
    12901 cagccagaaa tgtataaaga aagtgtaaat tttccaattg aatttcaaga aaaatcagac
    12961 tgtctcgcag acgtggttga gttgtctgcc aatcaaagag cgcaccaaac agtgctaatc
    13021 caagtggact ggccaccatg gtaatcgttg ttatcgttga catcagacgt ccttgtatat
    13081 gcgttggaac ttctcgctga agccagacca tataaaaagc aaaagaaaac attaatcctt
    13141 aaaaacggac ttatgaaaaa taatacatgc cgtttgggat ttacaattag ctaccaaata
    13201 agaggttatg acaaaagact tttcacaatc ccttcttccg aataaacggt gttctcctgc
    13261 caactcctcg gtcacaagtc acaatattta tcaaacatga gaagcaattt ttgtaaattc
    13321 cttcttattt cttgaagctg accacttctg tcacaacctc agaagcggat aaataatatt
    13381 gtttgatgat actttatctt tttcccgttt gtttatagat cgaatgatat tatagaccaa
    13441 gaatcctacc tattaacata gctaaaaaca caaaaaaggc actttccaaa ttgtggaaag
    13501 tgccagaaaa gttaggatta aggtaactgc gctcagcatt gcttcgccca gtacgattct
    13561 cgtaactcgt gaactcgaac gataatcgca attaacgttt tgagaactgt gaagctttac
    13621 gggctttctt aagacctggt taaacaaaac tctattttat tgcattttac aggaatgtat
    13681 ttcatgcgaa attaataagt ctttgtttat agagttttac tagggtataa aaagttgtat
    13741 gttatcatta cctcaaaaca ttacctcagc ttggcgagtt ggttgattga agaataaatc
    13801 atttatctag atatctcaat aaaaaatgat ttctaagaaa cttgtatttt cgtgttatta
    13861 tttttttgcc ctatcaaaat ttctaattct tctagcgtaa tatgattatg acacataatc
    13921 gattcaatta cttcaatacc tatttttttc gagaaagatc ccttttgttc
//
LOCUS       DAZKNO010000066        13902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000066 DAZKNO010000000
VERSION     DAZKNO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.067"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..850
                     /locus_tag="ACG8MY_001460"
     CDS             71..850
                     /locus_tag="ACG8MY_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0430656.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            843..1160
                     /locus_tag="ACG8MY_001461"
     CDS             843..1160
                     /locus_tag="ACG8MY_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0430657.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1404..3014
                     /locus_tag="ACG8MY_001462"
     CDS             1404..3014
                     /locus_tag="ACG8MY_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0430658.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            3171..4019
                     /locus_tag="ACG8MY_001463"
     CDS             3171..4019
                     /locus_tag="ACG8MY_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0430659.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            4250..4729
                     /locus_tag="ACG8MY_001464"
     CDS             4250..4729
                     /locus_tag="ACG8MY_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0430660.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            4762..6990
                     /locus_tag="ACG8MY_001465"
     CDS             4762..6990
                     /locus_tag="ACG8MY_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0430661.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            7119..8762
                     /locus_tag="ACG8MY_001466"
     CDS             7119..8762
                     /locus_tag="ACG8MY_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0430662.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(8835..9380)
                     /locus_tag="ACG8MY_001467"
     CDS             complement(8835..9380)
                     /locus_tag="ACG8MY_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0430663.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(9403..9888)
                     /locus_tag="ACG8MY_001468"
     CDS             complement(9403..9888)
                     /locus_tag="ACG8MY_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0430664.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(9878..10690)
                     /locus_tag="ACG8MY_001469"
     CDS             complement(9878..10690)
                     /locus_tag="ACG8MY_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0430665.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(10829..12361)
                     /locus_tag="ACG8MY_001470"
     CDS             complement(10829..12361)
                     /locus_tag="ACG8MY_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0430666.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(12470..13501)
                     /locus_tag="ACG8MY_001471"
     CDS             complement(12470..13501)
                     /locus_tag="ACG8MY_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0430667.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         4452 a         2539 c         2725 g         4186 t
ORIGIN      
        1 gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg taattaaagg
       61 agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc atgcagtgat
      121 gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt tgcaaaaagt
      181 tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg gtggcgcgca
      241 gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat tattgatggt
      301 tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt ttatgaaaaa
      361 agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa actatgcggt
      421 gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt tatatgtcaa
      481 ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt tcggctcggt
      541 gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca ttttcatgtt
      601 tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag gcgtattagg
      661 ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa cagtcattgc
      721 ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta agaaactaag
      781 agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg aggccaatca
      841 ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg gcatatatcc
      901 cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg ttcagtgact
      961 ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat gttttcatta
     1021 ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt ctagtagcag
     1081 gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt gcgtctgtct
     1141 ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg cgacataagt
     1201 tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg caagtttatt
     1261 cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta ttttaggaat
     1321 tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt ttattttatg
     1381 aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca ttttggctaa
     1441 cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc tggctaagta
     1501 tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt ggctatcttc
     1561 cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt gcaaaaaaag
     1621 tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt agaagtacga
     1681 tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta tttgctcctt
     1741 tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct atcagaagtt
     1801 tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat ttccaaggaa
     1861 aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg cgagtgatcg
     1921 ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata ttagaagctg
     1981 tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc catatgtata
     2041 tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc agccgttatt
     2101 tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta ccgtttattt
     2161 ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg aaaccactgc
     2221 ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc agccgtgctt
     2281 ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca gtagctataa
     2341 gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc gaaaaaacag
     2401 taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact ggaatgtctt
     2461 tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt gtcggatatt
     2521 tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta tctacgatgc
     2581 ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca attatcctga
     2641 aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg agcttatcaa
     2701 atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc aaagaatacc
     2761 gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc tccaccctga
     2821 ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg tcaatggaat
     2881 caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc ttagtatttt
     2941 tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa catcatagca
     3001 gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg gcaactcgta
     3061 tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat cttacttatg
     3121 atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac atgaaacttg
     3181 cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat cccgacttat
     3241 tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga atcgatatcg
     3301 aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact acttcacaac
     3361 ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg tatgacacga
     3421 ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta tttgcgatga
     3481 aagatgacat cagcggagta acggtcattc cttatgactc taagatcact agtatgccga
     3541 tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc cttgatgaaa
     3601 tacttgcaca tatcgatcga atccgagata atacctacgc ttatctgatt gtagatgatt
     3661 tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt ggcagcttat
     3721 taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc gaaaaaatcc
     3781 gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga aataacgaaa
     3841 tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta gcgattgcgg
     3901 agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt catttcggac
     3961 cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca gcgcaataat
     4021 tccttttatt caaaaaacat accaaaaata ttccaacatc atcttggact ttctacatgt
     4081 tactaatgag cgtgtaaaag aacgagatgg tgtttttatt tctatcaatg aagctctttt
     4141 tctttgcaat cctcacttca atcctctata atgtcgcata tagtttagta aatgagtaaa
     4201 ctactaattt acttatttac taaaaataaa aggatgagga gttaaaataa tggaaatacc
     4261 aacaaattta aaacataaac cagtaatcgt tgtagaagac tatgataaag ttgatggaag
     4321 aaatgcatta aatacggatg caaaagggct ttcactgggc cttgctcagt ggaatgatcg
     4381 tggaaaagtc gatatctcag gcaaggtatg gcgacatact ggagaaaaat ggtcacgaca
     4441 gtcggaagaa ttaccaatca cacgtatttt agatttagcg atcttagctg cacaaggaag
     4501 tttatacttc caagatgctt atcgttatga aaaattttat gatccagaaa atccagtcgt
     4561 agacatcatt ggattacaag ggaaccgaat gacggtagaa gtagatacca aaaatccaaa
     4621 gatcgatgaa gacatcattt tgtattacga tacactacaa aaagacggag aactgattgg
     4681 ggaacgattc cgtattttaa aacgtctttt agatgagcta ggatattaat agacagagat
     4741 gatcaaaagg ggaagagaag catggagaat ttctcactaa cagaaaaccg aaccttttca
     4801 cggctagaaa agcaaatgat ctggaagaaa ccgaaaagcc atcggacaag tgcagaagaa
     4861 tggcggatcg cctccgaaat caaagggaat tggcaagatc ctgaaatgaa aatcagtaac
     4921 gtacttttgg aaggagatgc cgggtcgggg aagacacaat tagccaaagc actatctgct
     4981 gatctgcaac ttccctatac aaaagtgacc tgttttgcag atatggacaa atcagatgtt
     5041 ttcggagcct tattacctgt agtagattct aataaggaac aagaccaaga attactagaa
     5101 gctatttacc aaacagatac acttgaagct gtactgacac tgatccagaa ccattacggc
     5161 attgatcctt tttctgctaa agaaaaatta gcagagctag ttcaaaggat cgaagctgat
     5221 tatccagatg caatccatta cagctattac ccttctgaaa tcgttcgagc gattgaaaaa
     5281 ggatatttat tagagattca agagccgact gtcatccgag atgcctctgt tcttgtggca
     5341 cttaactcag cgctcgaacc aaatgggatg ttgaatttac cgacaggaat cattcgtcgc
     5401 catccagatt gcgtcattgt gatcactaca aatcgaaatt accaaggaaa tcgcccatta
     5461 aatgaatctt taagagatcg gatgcaacat gcggaaaaaa tggatcttcc tgccttagaa
     5521 gtcatggcag aacgtgcgat ggcgaaaact atgatccaaa aacaagaact tctcttaaag
     5581 atggcagaga ttatccgttt gttggatgaa actgcaaaag ctaacgccat caaaggtgta
     5641 gcaggaatgc gttcttattt ttattgggca aatacctata agcaaggaca agatttactc
     5701 cagtcgattt atccaaaagt tttgtataaa ttatccactg attcagatga attggcaatt
     5761 cttgaacaag ctttgtctga cagtggattg ctactggaac ttcaggacat attgagaaaa
     5821 gaaaaatggg gtcatcctca atctgagcat acaaaaggaa gaaccattag cgccgaagaa
     5881 gcaaatgaac gaaacatcac tgaaaatgct gaagaaattc tgcgcttaga tgaaaagaaa
     5941 tcgttgtctg ctgaagaacc aaatgaggga gaagagaaac aagaagaaac aatagaaaaa
     6001 aacgagattc ctcctcaaat gcaaaaagaa cgcagtagcg atatcgaagg aaaaaatcaa
     6061 aaagaacaac aaacaggaga tgattctcaa gaaatgtctg cttctgacat ggaagcacat
     6121 gataaggaaa tcaagaagca actgaataaa gaagcgcgac aaatcatgaa ggaaacgatt
     6181 catgaaaaag aaggtctcat cgttcaccga ccagctttcc aaggaaaaac gccggaagca
     6241 cgtgagctac acatgcaagt aatgccaata gtagaatcac ttagccggca aattttagag
     6301 ttactggata atgaacaaac agagacgtac caaaaaggaa aatacgaagg tcagcggttc
     6361 aatgcaagca aagtagcata tggtgatttg agaaattttg acaaaaagaa cccgccacat
     6421 gagcaacctt ctttagcggt agctgtacgg atcgatgaat ctgggtcaat gatacgggat
     6481 gatcggatcg aagcggcaaa aaaagcagcg attgccattg ccgaattttc taagaaagtc
     6541 gatatcccat tgttgattta cggagataca gcagaccgtt cgtcaagaga aaagacttct
     6601 ttgttttctt ataaagaatt tgaagacgga tttcaatggt taaatgaaaa attagtcact
     6661 atgaaaccaa ggcaaaacaa tcgtgatggt gctgcacttc gtttagcagc agaaaaatta
     6721 aacagacaat ccgcaacaac caaactgttg cttaatatta gtgacggtca gccaaaagcg
     6781 ttgccggatt atacaggcaa aaaagcaaaa gaagatatcc agtctgtttt gaaagaatat
     6841 gaacgtcaag gaatcctatt tgtctccgca gctattggcc aagacaaaga agaaatcaag
     6901 tcgatctacg gtgaaagtcg ttttgtagat attactgact tgaatgaatt tcccaagcaa
     6961 atgattcaac tgattgcccg ctatctatga taaaataaag ctcaacgaac gcacttagca
     7021 gattcgttga gcttttcgtt tctagttacc ggtaacaagg atacttctaa aatagaatcg
     7081 gttataatca agagggaccg ttaaaggagt gaaacgatat gatacaagaa caaaaatggt
     7141 ggaaagaagc agtaggctat caaatctatc ctgctagttt caaagatagc aatggcgatg
     7201 gtcaaggaga catcaatggg atccgtgaaa aacttaatta tttgaaagat cttggtatcg
     7261 gctttatctg gatcaatccg atctatcaat ctccatttgt cgataatggt tatgacgtaa
     7321 gtgactatca agatatagaa gagagattcg gaacgatgga agattttgat ttattgctaa
     7381 aagaagcaca tgaattaggg atcaaagtga ttatggatct tgtcatcaac catagttcgg
     7441 atcagcatca atggtttgaa gaatcgaaaa aatctaaaga aagcccgtat cgcgattatt
     7501 acatttgggt cgacggggta gatggcaaag aaccgaataa ttggacatcg attttcggcg
     7561 gttctgcttg ggagtattct catgaaactg gacagtatta tctgcatgtt tttgcaaaag
     7621 aacaaccaga cttaaattgg gaaagcgaga aattgaaaga agaactattc aatatgatcc
     7681 gatggtggtt ggataaaggg atagatggat ttcgtttaga tgcgatttct catgtaaaaa
     7741 aagatgaata ttcagtaaaa gccacagaga accctttctc accctttcaa aatgtctcag
     7801 gaatcgaaga acatttgacc gatttgaaac atgtttttga agagtacgat atcatgactg
     7861 tcggagaagc aagtggtgtc actgcagaag aaggaccaca atgggtaggg aaagacggtt
     7921 atttcgatat gatattcgaa tttgatcata tccatatctg gcagcaagaa aaagaagggc
     7981 aattagatgt ccttaaattg aaacttgcat taagcgcttg gcaaacttca ttggatggta
     8041 tcggatggaa cgctttatat atggaaaacc atgatgttcc tcgtgccgtt tcagtgtttg
     8101 gagatactcg tcccgatttt tgggctatgt ctgcaaaagc aatcgctatg atgtatttct
     8161 ttttgcaagg tacgccattt atttatcaag gacaggaaat cggaatgacg aatatgccct
     8221 ttgaatcgat cgatcaagtt gatgcagtag attcgaaaag actttataaa aggctgcttg
     8281 cggaaggtaa gacaagagaa gaagcattgg atatcattcg tgaaacaacg agagacaaca
     8341 gcaggacacc catgcaatgg acttcggaac aatatgctgg tttttctaca catgaacctt
     8401 ggctgatcac taatccaaat accaaaacga tcaatgtaga acaacaagaa tatgaaccag
     8461 agtcggtcct gcaattctat aagaacatga tccgcattag acaaaccaat aaaggtctga
     8521 tatacggttc ttataaagaa tatcttcatg aacatccaca actttatgtc tatgaaagat
     8581 atttagaaga tgaacaatat cttattatgg taaacttaac agaatcactt gctgactatg
     8641 aacttccaaa agaagcagat cagtcctgga cattactctt gtcaaattcc tctagcggag
     8701 aatttgaagc taaaggcata ctagcacctt atgaagctcg gttatataaa acaaacaagt
     8761 aaaacttcct cgttaaagtt cattagttgg actttgcgag gaagtttttc agattgctct
     8821 gtcaattact catttcaata attcgctcta ggaccaccga tatatttagg acgagagcga
     8881 agggctgtca cgtgagcttg tccaagggtt ttcaaacgag gtctgacagg tacttggaca
     8941 tgtttgatat gcataccgat tgatgtgtca ccgatatcga taccggcttg tccaactacg
     9001 tgttccactt cgacaggatc attgaactgg tcaaaagcag caacagaaca agcacctcca
     9061 gcatgtagtg caggaacaac agagacaatt tcgaaatttt ttgcttctgc cactgttcgt
     9121 tctactacta atgctcggtt caaatgttca caaccttgga cagctagcgc aatttcttta
     9181 ggatccaaca actcttttaa cgtacggatg atccattgac caacttccgc actggaattt
     9241 tttccaatat gtcctccaac gacttcgctt gtgctgcatc ctaaaacaaa aagatcacct
     9301 tttttcaagt gtgcctcagc caatatgtca ttaactattt cagtcaattg gtcttttaat
     9361 tctttttctg acaacttcat tttctaaaac cttctttttc aattaatatt gcttccgtgc
     9421 ttgaatacgt tgaagacttg cagttatagg aaaggcaagt cccattccaa tcagattttg
     9481 gaagatgttt cctggtatag aagccactcc agccggccaa tcatataaaa tccaaccggc
     9541 gataccatag ccgatgatca taactaagct tcctaataaa agccctaatc cccacatacg
     9601 agtagaagat ttgctgaaag aaccagcaac atatccttgt aacccgtgga tcactaatga
     9661 aaaaactgcc cactgcggat atcctgaaat caaatcgatc aatagaccac tggaagcacc
     9721 aacaactaat ccacccgttg gaccataaag aaaagcagcg gtatagatcc cggcctcgca
     9781 taatgtcaca aatccattcg tagccggtac agggatcaaa attgttaatg aaagggcaac
     9841 tgtcaacgca atcagcatag catagatcgt taattgctta gttttcattt ctcattcctc
     9901 ctttttgaat accgccagac cagacacttc cggtatgatc gttcatcagc acgccattag
     9961 aaatacaatg ggcaacaaat tttttacttt ctgctactgc tttggtaaaa tctgccccta
    10021 acacaagatt cgctgttatt gcagaagaaa aggcacaacc ggccccgttt actgttgtag
    10081 tgtcgatttt tttatgagaa aatacctcat aatgatcaat tgaatagaga agatccacag
    10141 cttcatctcc agccatcctt gtgccgcctt tgatcacaat gttttttgtt cctgaagcag
    10201 tgagttcttt agcgagatgg atcatttctg ttaacgaaga gggaagagct tgaccagaaa
    10261 gtaaagcagc ttctgccaaa ttaggcgtaa tcatcgtaac aagtggaaaa agttcttcga
    10321 tgagctgcga aatatacaat tgattgatca cagcttcagt ttctttgaat gccaaaacag
    10381 ggtctaaaat gattgggata ttttgttttg tttttatgaa gtctcgaaca accatgattg
    10441 cttcacttga attcaataac ccaattttga taccatctaa tgaagtcatt ttatcaatcg
    10501 aaactaactg tcgtttaagc aattccaaag atatagattc cagcataaat cgctcattaa
    10561 tatctagtgc ccctatagaa gtcaaggcgc ttagtccgaa tagaccaaaa ttctcaaaag
    10621 tttttaaatc tgtttggatt cctccaccag caaatggatc agaaccgcca attgtcaaga
    10681 ttcttttcat aacattttcc tccatgaata agagtataac gaaattttca aaaaagaaaa
    10741 acagccaatt ggcatatttt tatctcatcc aatttaaaaa aacaaaaaga aaagaaagac
    10801 caaaatggat ctttctttcc ttaaatagtt aggacttttg tgattttaat tctttctcga
    10861 tttgaagccg ttgcactttc attgtagctg ttcgcgggat tgcatcaaaa tcacggatga
    10921 tagggacgtt caagcgagga agttcatgga cttgctccca ccatcgattc caatccattt
    10981 ctttatcagg agctaaagca ataatcggtt gtggagaatg atttttatcc cttacaatca
    11041 ctacttctgc caaaaaatct aacgaatcta ataaaaagtc ttcaatagct aatgtactat
    11101 tgatcgtttc gatcaaatcc acttgccgat ctttcaaaaa taggtggcca tgcttatcca
    11161 taaaaccgta atctccgcta tcccaccaat cgccataaac attttcctgg aaccgtgcat
    11221 cttccttgta ataagtcaaa gcccgtcctt tagacagtaa ttgtatatga ccatctgtgt
    11281 tttcaggtag gacattccca actgaatctg taatccgagc gcttgtcatg tcttccagtc
    11341 caacgcccat atctcttgca tcacttgttt tcaaactctc tagtgtatga gccttaagaa
    11401 tcattggtcc gcattcactt tggccatata cttgaagaaa taccgcatcc tgtgccatcg
    11461 acgttcgcaa aaatgtcttc attgtctggt tgtttatagc atcaaaagtg gagtgatagt
    11521 atcggatacc cgaaaaagct tctggatgct ccttagcagt aaaacgccac tgaacaaagt
    11581 tattcggatg ggtctctagt gcaattggcc ggtgagcttc caacatatga acaacttttg
    11641 aactttgtgc gtttgctaaa ggcatcatag gaaatcccat ggacatcaaa gaagagatac
    11701 caatattgaa tctggaatgc actggagaga tgtgaaaagc gattaatttc ttttccgaaa
    11761 tttttgtgaa aactgttttt tgccatttcg tccgccatcc catagaatga gcagaatgac
    11821 aaataagttt agggatacca gttgtaccag aagtatgggt catataagaa atttcatttt
    11881 tgtctaaaaa tgattgagaa acagacaaag caggttgttc cattaaataa agaataggaa
    11941 tctgttttga ttttgttcca tttgaaattt ctctcactcg cttttttgtc tcatcatcaa
    12001 ataaaatgaa agggtcttct aaacgatcga taaatacttc cattgttgtt gttggtagat
    12061 gataagaagt catgacaggc actgcaccaa ggtaagacac agaaacagct agaaggtatg
    12121 tgtcaaaagc ggaactctta taaataataa ctttgtctcc ggatttcact ccaagatgag
    12181 ccaattgata agcccgtttc aacacacttt cgcagcattt tttgtaagtt gtttcaagtc
    12241 ctaattctgg gaaagctgaa agtggctcat caaagataat cggggtctcg gaaaagttag
    12301 ctgcagcttc ttgaaaattc gtaaataagt tcaaagggga atacgttatg gtctccatca
    12361 ttcacactcc attatctttt gttaagtaat atacaaattc aagataatcg taacataatc
    12421 ctttatataa taacatagtt tattgtgaaa aaaagatcat acatttgttt tatatggaag
    12481 aaacccgaac gaatgccgct aattcgtgat aagaaaaatc aaccatcttt tcccagatat
    12541 cttgaaaaga atgggatgag aggtatcgat taccacataa ataatttacc gcgtcagcat
    12601 catctaaata tagttctgca taataacgat atgtttttct gcatagttct ggcgatacgt
    12661 ttttatatac tgtaattttg ctcaaaattt ggcgaatagt ccgaggagaa atttgctgat
    12721 catgtgagga acgaaaaaca aaagaatttt cgtccgtatt ttgtgctgtt aacttaaaat
    12781 agttctccag aaatggcatc gcttcttttg gtattggtac tttattcagt ctgttttctt
    12841 ttctattcag attaagaatc tgcttggtca aagatacatc tttacgttta agttctacta
    12901 cttcatggat ctttaatccg caatacagaa gaatggcagt aattgcaagg ttcctcggtc
    12961 tattcttttc ttcaaagcta gcttctctgc ttgtttgaaa gccttcaatc gggataaaca
    13021 aatgctcaaa atctttgaaa agttctgcat cgatatatcg cggagctgct ttggttgttt
    13081 ccggtaaact tgtacccaaa agagaaatct cttcttcaat cagtcctttt tgatacaaaa
    13141 atcgtaaaaa atgatttaat gaatattgct tccttgaaat cccgtaatag ctattttcaa
    13201 acaattgctc cgtttcttgg ttcgctgtag tttggaacga acgagtatgt cgatagattt
    13261 tagctaagaa attctgataa tcttgttttt ctaaatggat aatcagctga tcatccactt
    13321 cttcagaaaa tacttttctc aaataatcag taaacagatg aaaatctttt gcgtattcat
    13381 agcgactgcc taatgaaagg gaagaacgat tttcttctaa aaactgttga aagacggaag
    13441 ggagcaagtg tgtacgttgc tttaactttt cttgcagttg ttttctagtt ttgctgtcca
    13501 ttacaaattc cctccaatag tgttcatttt ctatatttta ccgcatttca tcaaatgaag
    13561 atagacagtt tgtcttgatc ttaggaagca aatttttaac taagtactag ctaaaagata
    13621 tgaagtaaga agatacattt ttagttattt tattagtgca gtcattcttt tgatctttta
    13681 attgatttat cctcttttta gctatgtggt tacttcatga gcaatgaaat ggatttggtt
    13741 cgttctaatg gcaggaggct ctttctttta tttccgaaaa attagaaaac attttttcag
    13801 aatcgattgg ctctccattt attcaataat ggtttgtcta acgatattgt tgggataatt
    13861 cttaaagaag tattagaaat agttggtact tcattacttt ac
//
LOCUS       DAZKNO010000067        13747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000067 DAZKNO010000000
VERSION     DAZKNO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.068"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            363..533
                     /locus_tag="ACG8MY_001472"
     CDS             363..533
                     /locus_tag="ACG8MY_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430668.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            547..1149
                     /locus_tag="ACG8MY_001473"
     CDS             547..1149
                     /locus_tag="ACG8MY_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0430669.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1165..1842
                     /locus_tag="ACG8MY_001474"
                     /pseudo
     CDS             1165..1842
                     /locus_tag="ACG8MY_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2153..2650
                     /locus_tag="ACG8MY_001475"
     CDS             2153..2650
                     /locus_tag="ACG8MY_001475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFQ0430670.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2651..3067
                     /locus_tag="ACG8MY_001476"
     CDS             2651..3067
                     /locus_tag="ACG8MY_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFQ0430671.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3087..4631
                     /locus_tag="ACG8MY_001477"
     CDS             3087..4631
                     /locus_tag="ACG8MY_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0430672.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4774..4998
                     /locus_tag="ACG8MY_001478"
     CDS             4774..4998
                     /locus_tag="ACG8MY_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430673.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5013..5882
                     /locus_tag="ACG8MY_001479"
     CDS             5013..5882
                     /locus_tag="ACG8MY_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0430674.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5863..6597
                     /locus_tag="ACG8MY_001480"
     CDS             5863..6597
                     /locus_tag="ACG8MY_001480"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0430675.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6630..7538
                     /locus_tag="ACG8MY_001481"
     CDS             6630..7538
                     /locus_tag="ACG8MY_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0430676.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7535..8077
                     /gene="sat4"
                     /locus_tag="ACG8MY_001482"
     CDS             7535..8077
                     /gene="sat4"
                     /locus_tag="ACG8MY_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFQ0430677.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8170..8964
                     /locus_tag="ACG8MY_001483"
                     /note="aph(3')-IIIa"
     CDS             8170..8964
                     /locus_tag="ACG8MY_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFQ0430678.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9240..9371
                     /locus_tag="ACG8MY_001484"
                     /pseudo
     CDS             9240..9371
                     /locus_tag="ACG8MY_001484"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9447..9940
                     /locus_tag="ACG8MY_001485"
                     /pseudo
     CDS             9447..9940
                     /locus_tag="ACG8MY_001485"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            10110..11006
                     /locus_tag="ACG8MY_001486"
     CDS             10110..11006
                     /locus_tag="ACG8MY_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0430679.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            11105..11314
                     /locus_tag="ACG8MY_001487"
     CDS             11105..11314
                     /locus_tag="ACG8MY_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0430680.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11331..11603
                     /locus_tag="ACG8MY_001488"
     CDS             11331..11603
                     /locus_tag="ACG8MY_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0430681.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            11605..12468
                     /locus_tag="ACG8MY_001489"
     CDS             11605..12468
                     /locus_tag="ACG8MY_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0430682.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(12731..13468)
                     /gene="erm(B)"
                     /locus_tag="ACG8MY_001490"
     CDS             complement(12731..13468)
                     /gene="erm(B)"
                     /locus_tag="ACG8MY_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0430683.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(13593..13676)
                     /locus_tag="ACG8MY_001491"
     CDS             complement(13593..13676)
                     /locus_tag="ACG8MY_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0430684.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         4871 a         2137 c         2855 g         3884 t
ORIGIN      
        1 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg
       61 tgtttgtttt tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta
      121 attaattatt agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga
      181 acgaagtgag ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg
      241 atcttttaag ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt
      301 tttagcagta taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta
      361 aaatgaaaaa atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg
      421 agcaacaata tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg
      481 aaattgaaaa agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg
      541 ttgaaaatgg ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga
      601 caactgaaag cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg
      661 gtcgaacgcc cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt
      721 gttactgaat tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa
      781 attcaaatta agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa
      841 gatgaaaatt taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg
      901 gaatctgaac gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa
      961 aaaggaaaat tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac
     1021 gctttcgatt tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt
     1081 aatcgccgaa cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa
     1141 aaacaatgaa aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag
     1201 gcattagcct acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc
     1261 aaagacccac tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa
     1321 ttagcagaac caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg
     1381 atttttcctg atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt
     1441 gttgctgaac ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa
     1501 ggtgaaaata tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg
     1561 tataaaagat tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat
     1621 ttgcaaccag gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc
     1681 gattggttga tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc
     1741 gtacaaggta cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa
     1801 cgccaggaag caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt
     1861 tactaatagt ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc
     1921 attaggtgat gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct
     1981 cagaagatat aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa
     2041 agtagttagc aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta
     2101 ccttatccac gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt
     2161 tttgcgagat ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag
     2221 tcataagcta atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga
     2281 ccgtgaaaga atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac
     2341 ccttattgca ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa
     2401 tgttttagat agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc
     2461 tgcgagtggg gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga
     2521 taattttaat ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt
     2581 aaattccagg gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt
     2641 ggtgagataa atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt
     2701 cgatgaaaag gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc
     2761 ccttacagtg gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat
     2821 tttgaaaaac gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat
     2881 atttgataaa gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga
     2941 gcttgccgct ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa
     3001 taagatgcca gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga
     3061 ggaataaagt gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta
     3121 cagccgcaaa agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag
     3181 aaacagaaga acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa
     3241 agaaaaataa tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga
     3301 acactaaaaa gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg
     3361 acatggttat tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat
     3421 acattagaca gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca
     3481 ccacggatgc caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa
     3541 gccagagcct ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg
     3601 tgcaggtcaa ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta
     3661 ttgtccccga agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga
     3721 gtctagtggg cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac
     3781 caagatggcg accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg
     3841 cccttctgca aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag
     3901 ggcatgttcc ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat
     3961 ttttgcaggc gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga
     4021 acaaaagaat ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg
     4081 gcgatattta tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat
     4141 gcgtgactag aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg
     4201 aactttatgg tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga
     4261 taaagacact ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt
     4321 cagagattaa caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg
     4381 gtcaagacta tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc
     4441 ttttagtaga agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat
     4501 ttatccgcaa gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa
     4561 tccagagcgt tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa
     4621 tggaaatatg aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc
     4681 ttttgcttgc tgattataat aatattttaa taactattgt gtttatcaac caaatggttt
     4741 ataatattct tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa
     4801 ctgtgtgagc aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca
     4861 cagaatttca ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt
     4921 gctgatgtgc taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata
     4981 aaaatatcta acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa
     5041 cagagaaatt atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa
     5101 gaggcaccca tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat
     5161 ttgacctgac agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc
     5221 ttgttgtacc tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa
     5281 acgggtgtca tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag
     5341 atacggagca aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa
     5401 gtgctatgta tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct
     5461 tatgcgaatt aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga
     5521 acttttttat atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag
     5581 aggataaata ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac
     5641 tatttgcatg taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa
     5701 cttttgaata taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg
     5761 gtctttctct ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa
     5821 ttgtatcgga gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat
     5881 gatgataata tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct
     5941 ggtgcgggag aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg
     6001 cttgatttag gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc
     6061 tctgtagtag gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat
     6121 tttccacaga ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc
     6181 tttgatgtaa tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata
     6241 aaaaagatat ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct
     6301 gtttttactg ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat
     6361 ttcccggtgg acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag
     6421 gttacaaaat atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt
     6481 ataataaatc agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg
     6541 gcggatgaaa tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa
     6601 tatagaaaaa ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag
     6661 tactttcttt agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg
     6721 caaatattaa tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg
     6781 atatagaacc gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga
     6841 tgcaaaagcc ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta
     6901 tgctatttga tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata
     6961 attacctaaa gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa
     7021 gggacatagt tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg
     7081 atgattgctg caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta
     7141 aggaaatttt atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa
     7201 tgatatcatg gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata
     7261 agtttattga aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga
     7321 tggattccta tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg
     7381 tatccggtga ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa
     7441 caaaatatac cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca
     7501 catatgccgc tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca
     7561 cctgaaagat atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag
     7621 gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag
     7681 ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa
     7741 tactgataag ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact
     7801 gcgaaaaaat tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag
     7861 ggggcaaggc ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa
     7921 cttgcatgga ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca
     7981 taattgtggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
     8041 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
     8101 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
     8161 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
     8221 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
     8281 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
     8341 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
     8401 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
     8461 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
     8521 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
     8581 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
     8641 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
     8701 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
     8761 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
     8821 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
     8881 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     8941 attttactgg atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca
     9001 agctttttag acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt
     9061 ttatatcttt tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg
     9121 gtgaagaggt ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca
     9181 gctagcttaa gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt
     9241 tgaacaggat taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc
     9301 ctgccaaaga aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc
     9361 ggacacttga agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg
     9421 tagagggctt cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta
     9481 cagcagggaa atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag
     9541 ctatgtataa aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg
     9601 agaacagtat taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta
     9661 ttgcagcaat tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat
     9721 caggagggca agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac
     9781 agaactggta cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga
     9841 tcggattaaa agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga
     9901 acaaatatta aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa
     9961 aaattgggga aaagggatat attgatttta tatctttctc aaaataaata taaaagccct
    10021 ttaaagaggg cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat
    10081 cacaaatcac ttgtgatttg tgattcttaa tgatacaata ttactataca aaaaaagaat
    10141 ggggcgtagt tatggagaag gaaaaactaa aaatacttga agaattaaga cgtattttaa
    10201 acaataaaaa tgaagcaatt attatcttga ataattactt taaaggtggt gttggaaagt
    10261 ccaaattatc gactatgttt gcttacttga cagacaaatt gaatttaaaa gttttaatga
    10321 tcgataagga cttacaggca acattgacaa aagacttagc aaaaactttt gaggtagaat
    10381 tgccacgtgt caatttttat gaaggcttga aaaatggaaa cttggcttct tctattattc
    10441 atttgactga taatttagac ttgatccctg gcacgtttga tttgatgtta ctgccaaaat
    10501 taactcgctc atggacgttt gaaaatgaaa gtagattgct tgctactctt ttagcacctt
    10561 taaaaagtga ctatgatctt attattattg atactgtacc aacgccaagc gtttatacaa
    10621 ataatgcaat cgtggcgagt gattacgtta tgatcccttt acaagcagaa gaagaaagta
    10681 caaacaacat tcaaaactat atttcctatt tgattgattt gcaagaacag tttaaccctg
    10741 gactagatat gattggtttt gttccttatt tagttgatac ggacagcgca acgataaaat
    10801 caaacctgga agaactgtac aaagaacata aagaggataa tttggttttc cgaaatatta
    10861 tcaagcgaag taataaagta agtacttggt ctaaaaatgg cattacagaa cacaaaggct
    10921 atgacaaaaa agttttgtct atgtatgaga acgtattttt tgaaatgatt gagcgaatca
    10981 ttcaattaga aaatgaaaaa gaatagaatc acaaatcaca agtgattaat cacaaatcac
    11041 ttgtgatttg tgataggtga tgataaaata aatagtaaga agaaatagaa agaagtgagc
    11101 gatcgtggga aatttaggcg cacagaaagc aaaacgaaat gatacgccaa tcagcgcaaa
    11161 aaaagatata atgggggata agacggttcg tgttcgtgcc gacttgcacc atatcataaa
    11221 aatcgaaaca gcaaagaatg gcggaaacgt aaaagaagtt atggatcaag ccttagaaga
    11281 atatatacgg aaatatttac ctgacaaact ttaaaaggag tgaaaatgaa atggcagtta
    11341 cgtatgaaaa aacatttgaa attgaaatca ttaatgagtt atcggcaagt gtttataatc
    11401 gggtattaaa ttatgttttg aatcatgaac tagatactaa aaatactcgt ttactagaag
    11461 tgaatctttt aaatcaatta gaagtggcac aagaagttga tttatttcaa caaccatttg
    11521 aagaattaca agctattcat gagtattggc ggtcaatgaa tcaatattca aaacaaattt
    11581 tgaataaaga gaaagtggct taacatggcg aatattactg acttcaccga aaagcaattt
    11641 gaagatcgtt tagaaaagaa tgttgaacga ctaactaaaa atagactagc ggttgaatcg
    11701 ccaaccgctt ttttacttgg tgggcaacca gggtcaggga aaaccagttt gcgatcagca
    11761 atttttgaag aaacacaagg gaatgttatt gtcattgata atgatacctt taaacaacag
    11821 cacccaaatt ttgacgaatt agtgaaactc tatgaaaaag acgtagtaaa acacgttacc
    11881 ccttattcga atcgcatgac agaagcactt attagccgtt taagcgatca aggctataat
    11941 ttagtgattg aaggaacagg acgaacaaca gacgttccca ttcaaacagc cacaatgctt
    12001 caatctaaag gttatgaaac aaaaatatac gtcatggcag taccgaaaat tgaatcatac
    12061 ttaggaacga ttgaacgata tgaaaccatg tatgcagatg atccaatgac agctagggca
    12121 acaccaaaac aagcgcatga tattgttgtc aaaaacttac cgaccaattt agaaaccctt
    12181 cataaaacgg gcttatttag cgacataagg ctttacaata gagaaggagt gaaactctat
    12241 tcaagcttag aaacgccttc cattagtccg aaagagacct tagaaagaga attaaatcgt
    12301 aaagtatcag ggaaagaaat tcaaccgact ttggagagaa tagagcaaaa aatggttcaa
    12361 aatcaacacc aagaaacccc tgaatttaaa gcaattcaac aaaaaatgga aagcttgcag
    12421 ctacctacac caccaatacc caaaacacct aaacttccag gtatttaaac cttaaaaaag
    12481 gaaaagagta gttaccaaaa aacggtaact actctttttt tataaaaaca tttcctcatg
    12541 ttggggtccc gagcgcctac gaggaatttg tatcgataag aaatagattt aaaaatttcg
    12601 ctgttttttt ctaatttcat tttgaggtga attaattggt agttattaga ggtgccctat
    12661 acctaataat ttatctacat tccctttagt aacgtgtaac tttccaaatt tacaaaagcg
    12721 actcatagaa ttatttcctc ccgttaaata atagataact attaaaaata gacaatactt
    12781 gctcataagt aacggtactt aaattgttta ctttggcgtg tttcattgct tgatgaaact
    12841 gatttttagt aaacagttga cgatattctc gattgaccca ttttgaaaca aagtacgtat
    12901 atagcttcca atatttatct ggaacatctg tggtatggcg ggtaagtttt attaagacac
    12961 tgtttacttt tggtttagga tgaaagcatt ccgctggcag cttaagcaat tgctgaatcg
    13021 agacttgagt gtgcaagagc aaccctagtg ttcggtgaat atccaaggta cgcttgtaga
    13081 atccttcttc aacaatcaga tagatgtcag acgcatggct ttcaaaaacc acttttttaa
    13141 taatttgtgt gcttaaatgg taaggaatat tcccaacaat tttatacctc tgtttgttag
    13201 ggaattgaaa ctgtagaata tcttggtgaa ttaaagtgac acgagtgttc agttttaatt
    13261 tttctgacga taagttgaat agatgactgt ctaattcaat agacgttacc tgtttactta
    13321 ttttagccag tttcgtcgtt aaatgccctt tacctgttcc aatttcgtaa acggtatcgg
    13381 tttcttttaa attcaattgt tttattattt ggttgagtac tttttcactc gttaaaaagt
    13441 tttgagaata ttttatattt ttgttcatgt aatcactcct gaagtgatta catctataaa
    13501 taaatacaga agttaaacga tttgtttgta attttagtta tctgtttaaa aagtcataag
    13561 attagtcact ggtaggaatt aatctaacgt atttatttat ctgcgtaatc actgttttta
    13621 gtctgtttca aaacggtaga tgttttatct acattacgca tttggaatac caacatgacg
    13681 aatccctcct tcttaattac aaatttttag catctaattt aacttcaatt cctattatac
    13741 aaaattt
//
LOCUS       DAZKNO010000068        13706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000068 DAZKNO010000000
VERSION     DAZKNO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.069"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..902)
                     /locus_tag="ACG8MY_001492"
     CDS             complement(<1..902)
                     /locus_tag="ACG8MY_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0430685.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="ACG8MY_001493"
     CDS             1185..2153
                     /locus_tag="ACG8MY_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0430686.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="ACG8MY_001494"
     CDS             2266..2544
                     /locus_tag="ACG8MY_001494"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0430687.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="ACG8MY_001495"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="ACG8MY_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0430688.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="ACG8MY_001496"
     CDS             3001..3756
                     /locus_tag="ACG8MY_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0430689.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="ACG8MY_001497"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="ACG8MY_001497"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0430690.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="ACG8MY_001498"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="ACG8MY_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0430691.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="ACG8MY_001499"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="ACG8MY_001499"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0430692.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="ACG8MY_001500"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="ACG8MY_001500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0430693.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="ACG8MY_001501"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="ACG8MY_001501"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0430694.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="ACG8MY_001502"
     CDS             8493..9035
                     /locus_tag="ACG8MY_001502"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0430695.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /gene="atpA"
                     /locus_tag="ACG8MY_001503"
     CDS             9058..10614
                     /gene="atpA"
                     /locus_tag="ACG8MY_001503"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0430696.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="ACG8MY_001504"
     CDS             10628..11530
                     /locus_tag="ACG8MY_001504"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0430697.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="ACG8MY_001505"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="ACG8MY_001505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0430698.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="ACG8MY_001506"
     CDS             12973..13395
                     /locus_tag="ACG8MY_001506"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0430699.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2452 c         2988 g         3736 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag actttt
//
LOCUS       DAZKNO010000069        13617 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000069 DAZKNO010000000
VERSION     DAZKNO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.070"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..558
                     /locus_tag="ACG8MY_001507"
     CDS             28..558
                     /locus_tag="ACG8MY_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0430700.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(1041..1484)
                     /locus_tag="ACG8MY_001508"
     CDS             complement(1041..1484)
                     /locus_tag="ACG8MY_001508"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0430701.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(1500..2660)
                     /locus_tag="ACG8MY_001509"
     CDS             complement(1500..2660)
                     /locus_tag="ACG8MY_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0430702.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3167..4867
                     /locus_tag="ACG8MY_001510"
     CDS             3167..4867
                     /locus_tag="ACG8MY_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430703.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(4969..5802)
                     /locus_tag="ACG8MY_001511"
     CDS             complement(4969..5802)
                     /locus_tag="ACG8MY_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0430704.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(6106..8271)
                     /locus_tag="ACG8MY_001512"
     CDS             complement(6106..8271)
                     /locus_tag="ACG8MY_001512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0430705.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            8531..10825
                     /locus_tag="ACG8MY_001513"
     CDS             8531..10825
                     /locus_tag="ACG8MY_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0430706.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            10818..11483
                     /gene="pgmB"
                     /locus_tag="ACG8MY_001514"
     CDS             10818..11483
                     /gene="pgmB"
                     /locus_tag="ACG8MY_001514"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0430707.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            11476..12483
                     /locus_tag="ACG8MY_001515"
     CDS             11476..12483
                     /locus_tag="ACG8MY_001515"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0430708.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            12505..13524
                     /locus_tag="ACG8MY_001516"
     CDS             12505..13524
                     /locus_tag="ACG8MY_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0430709.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT         4135 a         2812 c         2475 g         4195 t
ORIGIN      
        1 ttttaattta taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt
       61 ggaactcact ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat
      121 gaccatactg ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac
      181 caagaagctg atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca
      241 acagggacag acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc
      301 tttttcccta aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat
      361 atcgcagaaa tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct
      421 gaatttgcta cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc
      481 gaagaaccat acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat
      541 tataaacgag taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag
      601 tattagagaa agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc
      661 gagggtcagc tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga
      721 gtaataagaa cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta
      781 ccgagaagca gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct
      841 gagtattaga gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat
      901 accgagggtc agctgattga acaccgttta tcaaagattg taacaactgc atattcacac
      961 agttaaatta gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca
     1021 ttatttcgtt attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc
     1081 tcctcattta ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag
     1141 aaatgaatag ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct
     1201 tttatttttt tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag
     1261 attcctgcga taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct
     1321 gctaagtaaa tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa
     1381 taagttgatg gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca
     1441 gattccagtt ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact
     1501 tattcattga ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc
     1561 tcaaaaaagt atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta
     1621 ggatcatcag ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc
     1681 ccatgatggt cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta
     1741 tgtttcaaaa tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat
     1801 gaaaaatcaa gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact
     1861 aacagtatga ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt
     1921 tcctgatttc cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct
     1981 gtcactagca aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag
     2041 tcttctcctt ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt
     2101 ttaacttgat aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct
     2161 aaacgatcta attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact
     2221 gaatgcgtcg aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt
     2281 tcaaggatcc taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata
     2341 ttgatacttc cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc
     2401 ttttcattga aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc
     2461 tcctccattt tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg
     2521 atacgttttt gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc
     2581 agtaacccta cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt
     2641 ttttgcttac tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat
     2701 tatatgggtt caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc
     2761 tgcatcaagt aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg
     2821 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg
     2881 ttatgctttg agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag
     2941 tgagcttatt accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc
     3001 tactttgttt cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt
     3061 atgtcgcatc ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata
     3121 ttttcatgaa ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc
     3181 taaagtttgc aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt
     3241 cattgctttg gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga
     3301 tgccccattc cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt
     3361 ccattatttt ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta
     3421 cggcccattt ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca
     3481 ttatcagatt ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt
     3541 actgagatat gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag
     3601 tacgtattct attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt
     3661 ttatccttta tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat
     3721 caaagtcgct gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt
     3781 agctttgatg tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa
     3841 agcactgctt caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg
     3901 ggcaggatta gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga
     3961 aatgagtcaa aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt
     4021 tcctatattg atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt
     4081 attccaaaaa atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat
     4141 tccatggacg acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt
     4201 tcgatttttc gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca
     4261 gtggtctgaa caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc
     4321 acagacgatc cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag
     4381 tcgacatact tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga
     4441 tatgtctacc agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa
     4501 ggagacaaaa gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac
     4561 ttttataaaa acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa
     4621 aactgttcat atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac
     4681 attgactgat tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat
     4741 caacgagctc acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac
     4801 tttgattggc tggtttacct tgctatgttt atttatttat catagataca agcttttacg
     4861 tgcttaggca aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt
     4921 tggagagttt tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac
     4981 atctaattcg atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa
     5041 aatccgttga tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa
     5101 tttttctgta ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt
     5161 ttcattcgcg acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata
     5221 accttgagag tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc
     5281 caaaaagtag ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca
     5341 ccaagaaaag tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat
     5401 caagatcttg cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg
     5461 actttccaat gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat
     5521 gtggctcatt tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa
     5581 acggtaggca aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat
     5641 tagcttcgca ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat
     5701 cacttcataa tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc
     5761 tggctcaagc cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt
     5821 catttcgaaa caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt
     5881 tctcctgcta actcctcagt aataagctca atggaccagg agctattttt tgtttggctg
     5941 tttttatcac ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac
     6001 tgctgcttct cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg
     6061 ctgttcttat gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga
     6121 gccgatcact ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt
     6181 atcgtttgta aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag
     6241 atccatctca gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc
     6301 aaaagctggc tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc
     6361 atctttcgtt tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc
     6421 gatcggcgca acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt
     6481 ttgtgagaat accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga
     6541 aactagttct tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga
     6601 aaccatttct ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt
     6661 caagacatct gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc
     6721 catcgcccct gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact
     6781 tacgcgtaac cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa
     6841 atgaatgatc tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt
     6901 ttcttcactg tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt
     6961 gatcaagaaa tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa
     7021 gttgataaga tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc
     7081 gatattccca aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc
     7141 ttggatcact gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa
     7201 tggttctgtt acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata
     7261 tcttgctttc ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat
     7321 acctgaagaa ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac
     7381 aaactcatat tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc
     7441 caaccataac ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc
     7501 atacgatccg ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa
     7561 tggcaatagt aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc
     7621 ttgtgattct gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac
     7681 cacagcaaga atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg
     7741 ctttccattg aaaaatgaca aagcatccgg caattttcta taattatagt acttattata
     7801 agccattgcc ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg
     7861 tgcttccaat acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg
     7921 ggtaaatgca tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat
     7981 ccgattgatt aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc
     8041 ccaacttcct cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc
     8101 aatattttca attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg
     8161 aattgatttc cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat
     8221 taaagccttt ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag
     8281 ctcctttacg caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat
     8341 gctttatttt ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc
     8401 ttctataatt ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt
     8461 ttgtctattg acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag
     8521 gaggagaaaa atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac
     8581 gacacaatta gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg
     8641 ttatatgggg atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac
     8701 ttatttggcg ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta
     8761 tccagaatac tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat
     8821 caacgatcag ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga
     8881 tatgaaaaat ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg
     8941 catttccttc gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac
     9001 tatagaaatg cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt
     9061 tcaaaacgaa gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat
     9121 agaagaaatc ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt
     9181 tactgttact gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca
     9241 cgaagatact ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg
     9301 gtcagtggaa aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc
     9361 agaaaaagct gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga
     9421 gcaaacagcg gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga
     9481 agaagcacaa caaggtattc gattcaattt gttccaacta ttctctactt actacggcga
     9541 agatgaacga ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac
     9601 ttactgggat acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt
     9661 aactaagaat ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca
     9721 gcaacaagga ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca
     9781 taacgagtgg gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat
     9841 ctacaattat acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt
     9901 tttagcagaa atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa
     9961 atacatgatc cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg
    10021 gtatacaaat acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta
    10081 tcaaaaagaa gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc
    10141 agagaacatg tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt
    10201 cttagataaa gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca
    10261 gcattggtct tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca
    10321 ggggatttat ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt
    10381 ctatgaacca atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc
    10441 agcagaatta ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga
    10501 tttggataat tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag
    10561 ctggttgtcg attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt
    10621 cgcaccattt ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg
    10681 tttattgttc atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc
    10741 tctagaaatt tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct
    10801 aagaaaggag gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg
    10861 atactgctga attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga
    10921 tcgaccacac attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc
    10981 ttttagctta tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga
    11041 aaagaaaaaa cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc
    11101 ccggggtcgt gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt
    11161 ctgcaagtaa aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg
    11221 ctattgccga cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg
    11281 cagcaaaagc tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg
    11341 gtatccaagc catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc
    11401 ttggagagga gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag
    11461 aaaagtggca tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa
    11521 ataaagaaaa tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc
    11581 cgcctgcacc aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct
    11641 tacgatcgtt tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct
    11701 gtagccggaa ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga
    11761 tcacaccata tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt
    11821 ttcaaaactc catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat
    11881 ccaggaccaa tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca
    11941 acctctgcta gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac
    12001 ctttcaggaa atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt
    12061 gtgcttgaat tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg
    12121 ccaatcgact ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga
    12181 ctagatgatg tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc
    12241 gagaagcatt ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt
    12301 tctacgactg gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac
    12361 ggattagcaa tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg
    12421 atcaacctcc atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa
    12481 taacaagaga aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc
    12541 ggtgttgcca cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca
    12601 acaaagaaga aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc
    12661 cgcaatctag gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca
    12721 aaagagcgga tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg
    12781 cggaattatg atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag
    12841 aacgtccagc gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat
    12901 agtcaagata ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga
    12961 cagccttatc atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt
    13021 aaacaagcaa cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac
    13081 acgactcatg aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg
    13141 gctggtcttc ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt
    13201 gagaaaacgt tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc
    13261 cttcgcacga tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc
    13321 attagcttca acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat
    13381 atcgatatct ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa
    13441 gaattgagca gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc
    13501 gttcctttag aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct
    13561 atggtcagct ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaa
//
LOCUS       DAZKNO010000070        13584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000070 DAZKNO010000000
VERSION     DAZKNO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.071"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..253
                     /gene="yaaA"
                     /locus_tag="ACG8MY_001517"
     CDS             11..253
                     /gene="yaaA"
                     /locus_tag="ACG8MY_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0430710.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            240..1364
                     /gene="recF"
                     /locus_tag="ACG8MY_001518"
     CDS             240..1364
                     /gene="recF"
                     /locus_tag="ACG8MY_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0430711.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1361..3307
                     /gene="gyrB"
                     /locus_tag="ACG8MY_001519"
     CDS             1361..3307
                     /gene="gyrB"
                     /locus_tag="ACG8MY_001519"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0430712.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3327..5798
                     /gene="gyrA"
                     /locus_tag="ACG8MY_001520"
     CDS             3327..5798
                     /gene="gyrA"
                     /locus_tag="ACG8MY_001520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0430713.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6034..6333
                     /gene="rpsF"
                     /locus_tag="ACG8MY_001521"
     CDS             6034..6333
                     /gene="rpsF"
                     /locus_tag="ACG8MY_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0430714.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6383..6895
                     /gene="ssb"
                     /locus_tag="ACG8MY_001522"
     CDS             6383..6895
                     /gene="ssb"
                     /locus_tag="ACG8MY_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0430715.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6921..7157
                     /gene="rpsR"
                     /locus_tag="ACG8MY_001523"
     CDS             6921..7157
                     /gene="rpsR"
                     /locus_tag="ACG8MY_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0430716.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7300..9273
                     /locus_tag="ACG8MY_001524"
     CDS             7300..9273
                     /locus_tag="ACG8MY_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0430717.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9279..9731
                     /gene="rplI"
                     /locus_tag="ACG8MY_001525"
     CDS             9279..9731
                     /gene="rplI"
                     /locus_tag="ACG8MY_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0430718.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10005..11372
                     /gene="dnaB"
                     /locus_tag="ACG8MY_001526"
     CDS             10005..11372
                     /gene="dnaB"
                     /locus_tag="ACG8MY_001526"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0430719.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12152..13444
                     /locus_tag="ACG8MY_001527"
     CDS             12152..13444
                     /locus_tag="ACG8MY_001527"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0430720.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4520 a         2465 c         2992 g         3607 t
ORIGIN      
        1 gggatgggca ttgaaacaac aagtcattct aaatacggaa ttcatgacgt tgggccaaat
       61 gttgaaagaa gtcacagtta tcggtagtgg cggacaagca aaatggtatt tggcagaaaa
      121 cactgtgtta gtcgatggcg aaccagaaaa tcgtcgtgga cgcaaacttt atcccggcat
      181 gatggttgaa gttccagaag taggaacttt ttttatggta aaaaaaggaa acgacgataa
      241 tgaggctgaa taagctgtat ttaaaaaatt atcgaaatta cgaagagctg aacatcgaat
      301 tttccaagaa cctcgtcatc tttttaggag aaaatgcgca aggaaaaacg aatcttttgg
      361 aaagtatcta cgtattagct atgacgcgaa gtcatcggac aagtagtgag caggaattga
      421 ttggctggac tgacgatcag gcgatgattc aaggagagat cacaaaaggt tcatccactc
      481 ttccattaga aatcttatta tctaaaaaag gccgaaaaac caagatcaac catatcgaac
      541 agaaaaagtt aagtacttac gtgggacaaa tgaatgtcat tttatttgca ccagaagatc
      601 tttctttagt aaaaggcagc ccgcagacca ggcggaagtt tttagatatg gaattaggcc
      661 agatcaatcc aatctatctt tatgatcttg tccaatacca agcaattttg aaacaaagaa
      721 accagtactt gaagcaattg aatgaaaaaa aacaaactga tttgctttat ttggatattt
      781 tgtccgaaca actagcagcg tttggcagca aagtattgaa agcaagagca cagttcgtca
      841 agcgattgga gttttgggct aattctttgc atcagcaaat cacccatcaa aaagagcagc
      901 tggaaatcga gtatctcacc gcagtcgatt ctttagaaac acatacgcaa gagcaaatcc
      961 aagaacaatt tctcgcactt ttgaaccaaa ataaaaagaa agaccttttt cggggaacaa
     1021 cgacagttgg tcctcatcgt gacgatcttt ccttttttat taatcagaaa aatgtgcaga
     1081 cttatggatc gcaaggacag caaagaacaa ctgcactaag tgtcaaatta gcagaaatcg
     1141 atttgattaa agaagagaca ggggaatatc caatcctttt gttagatgat gtgatgagcg
     1201 agctggatga taatcgtcaa ctccatctgc tagaaacaat cgaaggaaaa gttcaaacgt
     1261 tcttaacaac aacgacactt gaccatgtaa aaaataaaat gactgttgaa cctgagattt
     1321 tttatgttca acaagggaaa ctaaaaggag aagaaactga atgacagaag aaagaagttt
     1381 agtagaacgc gctaaagagt atgatgccag tcagattcaa gtacttgaag gacttgaagc
     1441 cgttcgtaaa cgacctggca tgtatatcgg ttcgactagt tctgagggtc tgcatcacct
     1501 tgtttgggaa attgtcgata attcgatcga tgaagtatta gcaggttttg caacgaaaat
     1561 ccatgtgatt attgaaaaag acaacagtat caccgttatt gacgacggtc gggggatacc
     1621 tgtagatatc caggcaaaaa ctggacgtcc tgcagttgaa acggtcttta ctgttttaca
     1681 tgctggagga aaattcggcg gtggtgggta caaagtttct ggtggacttc acggagtagg
     1741 atcttctgtt gttaacgcgc tttctacaca gcttgatgtc aaagtataca aagaaggaaa
     1801 tgtgtactat caagaatatc gtcgtggagc tgtagtggac gacttaaaga ttatcgaaca
     1861 aacggatcgc catggaacaa cggttcactt tacgccagac cctgaaattt ttacagagac
     1921 aactgagttt gatttttcaa aattggctac ccggatccgt gaacttgctt tcttgaaccg
     1981 cgggatgcgt atatcgattg aagacaaacg agaagaagaa ccagtcatca atgaatacca
     2041 ttatgacggc gggattaaaa gttatgtcga atacttgaat gcaaacaaaa ccgtgatttt
     2101 tccagaacct gtatatttgg aaggtgaaca gcaagatatc gcagttgaag tctctatgca
     2161 atatacagac ggctaccatt ccaatatcat gagttttgcc aacaatatcc atacgtatga
     2221 aggcggaaca catgaatcag gattcaagac tgctttaact cgtgtgataa atgattatgc
     2281 ccgaaaacaa aagctgatga aagaaaatga cgacaacttg accggtgaag acgtgcgaga
     2341 aggattgaca gctgttattt ctatcaagca tcctgaccct caattcgaag gacaaacaaa
     2401 aacaaaattg ggcaattccg aagtacggac agtcactgat cggttattta gcgaacattt
     2461 catgaaattc ttgctggaaa acccaagtgt gggccgtcaa attgttgaaa aaggattact
     2521 agcttccaga gctcgtctag cagcgaaacg tgcgcgtgaa gtaacgagac gtaaaggtgc
     2581 tttagagatt agtaatctgc caggaaaatt agcggactgc tctagcaacg atcctgaaaa
     2641 atgcgaattg ttcatcgtcg aaggagattc ggccggcgga tcagctaagc aaggtcgtaa
     2701 tcgagagttt caagccatct tgcctattcg tgggaaaatc ttgaatgttg aaaaagcaag
     2761 catggacaag atcttggcaa atgaagagat ccggtcttta tttactgcta tgggtacagg
     2821 gtttggagaa gattttgatg tatcgaaagc tcgctaccac aaattagtta tcatgactga
     2881 tgccgatgtc gacggcgcgc atatccgtac attgctgctg actctttttt atcgttacat
     2941 gcgtcctatt gttgaagctg gatacgtcta tattgctcag ccacctttat acggggtaaa
     3001 acaagggaag aatattacgt atgtacaacc aggaaaaaat gcagaagaag agttgaaaca
     3061 agtcgttgct tcacttcctg cctcaccaaa accaagcgtt cagcgttata aaggacttgg
     3121 agaaatggat gatcatcaac tatgggaaac aactatggac ccaagcaatc ggatgatggc
     3181 ccgcgtcagt gtggacgatg ctattgcagc agatcagata tttgaaatgt tgatggggga
     3241 tcgagtagaa ccccgtcgtg cattcatcga ggaaaatgca cactatgtga agaacttaga
     3301 tatttaatca tgaaggagga aaaagaatga gtgaagaaat cagagaaaac atccatgatg
     3361 tcaatctaac cagcgaaatg aaagactcct tcatcgatta tgcgatgagt gttattgtcg
     3421 cacgtgcact tcctgatgtc agagatggtc tgaaacccgt tcatcgccgt attttatacg
     3481 gcatgaacga attaggtgta acaccggata aagcccataa aaaatctgcc agaatcgtcg
     3541 gagatgtcat gggtaaatac catcctcatg gagactatgc gatctatgag tcaatggtac
     3601 ggatggcaca gccattcagc taccgttata tgctagtgga tggtcatgga aactttggtt
     3661 ctgttgacgg tgacggtgcc gccgctatgc gttataccga agcacggatg agcaaaatcg
     3721 ctacagaaat gctgcgtgat atcaataaga acactgttga tttccaaagc aactatgatg
     3781 atacagaaaa agaaccagtt gtactacctg ctcgcttccc gaatctttta gtgaacggaa
     3841 caacggggat cgccgtaggt atggccacga atatcccacc tcataacttg tctgaggtag
     3901 tagcggctat cgatttgctg atggataatc ctgatgtgac gaccaatgaa ttgatggaag
     3961 tattacctgg accagatttt ccaacaggtg gtttggttat gggaaaatca ggaatccgaa
     4021 gagcgtatga aacaggtaag ggttctatta ccgtacgtgc aaaagttgag attactgaga
     4081 tgccaaatgg taaagaacgg atcatcgtga cagaattgcc ttatatggtc aataaagcaa
     4141 aattgatcga acggatttct gaactgcaca gagacaagcg tatcgaaggg atcacagatt
     4201 tgcgggacga atcttcccgt gaaggtatgc ggatcgtcat tgacatccgt cgtgacgcca
     4261 gcgcatctgt tatcttgaat aacttatata aattgacatc cttgcaaaat tcatttggat
     4321 tcaatatgtt ggcgatcgaa aaaggtgtgc cgaaagtttt gagcttgaaa caaattcttg
     4381 aaaactatat cgaacatcag cgcgaagtca tcacaagacg gacagtcttt gaaaaagaaa
     4441 aagcagaagc aagggcacac attttagaag gtttgcgtat tgctttggat catatcgatg
     4501 aaatcattgc gattatccgc gggtctaaat cagatgacga agcaaaagcg acgatgatcg
     4561 aacggttcga tttatccgat cgccagtctc aagctatttt agatatgcgt ttacgccgat
     4621 tgaccggttt ggaacgggaa aaaatcgaaa atgaatacca agaactctta aaactgattg
     4681 aagatttaac tgacattctg gcaagaccag aacgtgttac agaaatcatc aagaatgaat
     4741 tagcagactt gaatcaacga tttggtgata aacgacgcac agaattacta gtcggtgagg
     4801 tcttaagtct ggaagatgaa gatttaatcg aagaagaaga aatcgttatt acattgacaa
     4861 acaatggtta catcaaacgt ttggcaaata atgaattcag agctcaacga aggggtggac
     4921 gtggtgtcca aggaatgggt atccacaatg acgacttcgt caaaaatctg gtttcttgtt
     4981 ctacccatga tacattgtta ttctttacga ataacggaaa agtgtatcgg gcaaaaggat
     5041 acgaaattcc agaatacggg cgcacagcca aaggaatacc aattatcaat ttactaggta
     5101 tcgattcgag tgaaagcatc caggcaatca ttgcggtgac gggagaagca gaagaaggcc
     5161 actatctctt ctttacgacg cgtcaaggta cagtaaaacg tacttctgtc actgcttttg
     5221 ctaacatccg aagtaatggt ctgatcgcaa ttggtttgaa agaagacgat gagctcgtca
     5281 atgtgctact gacagatggt caatctaata tcattattgg aacacataac ggctactctg
     5341 tcactttcaa agaagaagca gtccgcgata tgggacgtac agcttccggg gttagaggaa
     5401 tacgcctgcg tgaggaagat tatgtcgttg gggcagcctt aatgaaggaa gatcaagaag
     5461 tattagtgat cacagaaaaa ggttacggaa aacggacaaa agtatcggaa tatccagtga
     5521 aaggccgcgg aggtaaaggg atcaaaacgg cgaacatcac tgaaaaaaat ggtccactag
     5581 ctggattgac cactgtttct ggagatgaag acatcatgct gattacagat aaaggtgtca
     5641 ttatccgttt caacgtatcg actgtttctc aaactggacg ttcaacatta ggcgtacgtt
     5701 tgatgaagat ggaagctgat acgaaagttg tcacgatggc agcagtcgaa ccagaagctg
     5761 cagaacaaac agttgaagaa caaggatcag aagaatagcc atcaagacta attgtgtaag
     5821 ctagcaagaa tgaccgatta aaaaatctgg ttattcctgc tggctttttt gattagttga
     5881 atttctctta aaatattcga aactgttcat tctgtattgc ttttgaccaa aatagagtgt
     5941 ataatcagag cttgtgagta gtctaattaa ttagataact ctccttgctc ttaggaacct
     6001 aagagccgaa agtccacagg gaggtgaata tcaatggaaa acacgaaata tgaaatcatg
     6061 tacattattc gtccaaacat tgatgaagaa gcaaaaactg ctttaatcga acgtttcgat
     6121 gcaatcttga aagataacgg agcagaagtt atcgaatcaa aagattggga aaaacgccgt
     6181 ttcgcttatg aaatgaacgg ttaccgtgaa ggtatctatc acatcgttaa agtatcttct
     6241 ccatcatcag caaatgctgt caatgaattt gaccgtcttg ctaaaatcaa cgacgatatc
     6301 attcgtcaca tgattgttaa agaagaagct taattgttgt ttcatgtgaa acaatagaaa
     6361 acgatgagag gagatgacta ttttgattaa taatgttgta ttagttggac gtctgacgaa
     6421 agatccagat ttgcgttaca cagcaagtgg tacggcagtc gcaacattta ctttggctgt
     6481 gaatcgtaat tttacaaacc aaagcggcaa tcgcgaggct gattttatca actgtgtgat
     6541 ttggcgcaaa tcagcagaaa ctttagcaaa ctatgctcgt aaaggaacac ttttaggtgt
     6601 tactggacgt atccaaactc gttcttatga taatcagcaa ggacaacgtg tatacgtgac
     6661 agaagtagta gctgacaact tccaattgct ggaaagtcgt tctgcatctg aaaatcgcca
     6721 acaaagcggc ggattccaaa gttctggtca atcagctggc ggatttggcg gaaacaataa
     6781 ctctaatcaa acatctcaat catccaacgg tatgcctgat ttcgaccgtg atacttctga
     6841 cccattcggc tcaagttcaa cgatcgacat ttcagacgat gatttaccat tctaaacgaa
     6901 tttgatagga gggaaataga atggcacaac aaagaagagg cggacgtaaa cgtcgtaaag
     6961 tagactacat tgctgctaac catattgaat atatcgatta taaagatatc gaattactaa
     7021 aaagatttat ttcagaacgc ggtaaaatct tgccacgtcg tgtaactggt acaggcgcta
     7081 aaaaccaacg taaaattact gttgcaatca aacgcgctcg tatcatgggc ttgctacctt
     7141 tcgttagcga agactaataa atcctaggaa ttgaatgact gatccttttg atcagctatt
     7201 caattccttt ttttctgtaa aaataacctc tattcgttta gtcgctcgaa tgacgaaagt
     7261 tatggtaaaa tcaaattagt catatatggg aggaaaatta tggaaaataa aaacaatcat
     7321 ttatccccga aacctctctt ccttatcctg tttatattaa tcgaagtatt ggtagtctta
     7381 attttaccat ttcgttttgt tagcgcttcg gtcattctat tgatgaattt atggggtgtt
     7441 cgctattggt tgaaaatgaa acgttggctt attttaaata aacgtgaaaa aatccagcag
     7501 gcttcgactg tagctgaaac caatctagct tatgtctcag aagtcatgcc tgtaggagtg
     7561 attgcttatc agccatcaag ccaaaaaatc gaatggctca atccattagc attgaaaatc
     7621 aaagatcaaa gtggattaac agaggaagaa ttgctagata ctttttttac tgcaaaagaa
     7681 aaaaagaaaa accaagtcta tctagcagat aaaacatttc agtttgatat ggatacagta
     7741 aagggtgttg tttattttaa tgatattacc caaaaccagt tgttaaaact ccatcagacg
     7801 aacagtcagc cggtgattgg tattttatct attgataact atgcagatgc cattgataaa
     7861 ttggatgaca aagagatttc ttatttgaac tctttcgtca ctacactagt ttctgactgg
     7921 atgaatgaat accatgtctt tttcaaaaga atcaacgcag aacggttttt cttcgtagca
     7981 cagatgatcg atctgaaaaa gatgatggat aaaaaatttg atttgctgga tcgctttcga
     8041 gcagaagctg aaaaacagag ctcgcctatc acccttagta tggggattgc ttatggctca
     8101 agaaccctcg atgaaatcgg tggagaagct cagaataatt tagatgttgc tttagtccgt
     8161 ggaggcgacc aagttgtcgt gaaagaggta aaagaaggag caaaacctgt ttattatggg
     8221 gggaaatctg ctaagaatgt caaacgcacg cgtgttcgtt ctcgagcgat gagtaccgct
     8281 ttgcgtcgga tttttgatga aacaggcgat gtgtttatca tggggcacaa gtttccggat
     8341 atggatgctt taggctctgc ttttggtgtc agttgtctgg cacgcttcaa ccagaaaaaa
     8401 gcttacatcg ttatcaacga gaacgagatc attccagatg tagaacggtg tcttgaagaa
     8461 atccataagc atcctgaatt agaaatgcaa ctaatctctc cagaacgtgc catagagctg
     8521 aaaaaagaag aggacctcct cgtgatggtg gattaccata aaccttctct atcgatctct
     8581 cagggcctat atgagcaatt tgataagatt gtgatcattg atcaccatcg tcgtggggaa
     8641 gagtttccaa gtaaacctct gctgacttat atcgaatctt cagcttcttc agcttcagag
     8701 ctagtttctg agctgatcca ataccaaagc agcaaaacaa atcgcttaga tcgttttgag
     8761 gccacattat tgcttgcagg gatggttgta gatacaaata gtttcaatgt ccgtacaact
     8821 gcacgaactt ttgacgttgc cagctattta aggtcttgtg gagcagacgc atccttagta
     8881 caatatttat tgagctcgga tttagcgaca tacttggaga tcagcaattt gattgctgga
     8941 agcgactatg tgacttctga tattgttgtg gcggcaggga cagacgaaaa agagtataat
     9001 agtgttatag cagcaaaaac agcagataca ttgctttcga tgatcaatat caatgcagct
     9061 ttcgtcatta caaagcggac agacggcttg attgggatca gtgccagaag taccggcacg
     9121 atcaacgtcc aaatcatcat ggaatcctta ggtggcggag gacacttcac aaacgcagct
     9181 actcaactgg aaaatacgac agttgaggaa acaaaacaga agctatttgc tgcaatcaaa
     9241 gaaaatatga atcaaatata tgataaggag taatccaaat gaaagtaatt tttttacaag
     9301 atgtaaaagg aaaaggtaaa aaaggggaca tcaaagaagt tccagcaggt tatgcacaaa
     9361 attatctaat caaaaataac ttggcaaaag aagcaacgaa aagcagtatc agtcagttag
     9421 caggacaaaa gaaagcacaa gaaaaacacg aagctgaaat cttggaagaa gctaaaaagt
     9481 taaaagaatt cttggaaaaa gaagatactg tagtcgaatt gaaggcgaaa gcaggggaag
     9541 acagccgttt gtttggttcg atcccttcaa aacaaattgc tgaagggtta aacaaacaat
     9601 acaaaatcaa attagataaa cgaaaaatcg aattagaaaa tccgatccgt tcactaggct
     9661 acacaaacgt acctgttaag cttcatcatg aggtgacggc tacgatcaaa gttcatgtcg
     9721 tacaagaata acaaatcttg atagcgatgt tcagctggta agtataacca gaaggaaaaa
     9781 agacaaaccg aaatttatct gagaaaacag ctgcagcaat gaaagcaaaa gctgcaaatc
     9841 gatttcagat tgatttcggt ttcttttttg ggaacagcaa aatagcataa agaaaacgag
     9901 tataataaaa gagaactatt tttttattac actttcggta cgaatgatga cttttctctc
     9961 attttcttgt aaaatgaagc aattgaagac aaggagcgat acgtatgaat gaagtgtggc
    10021 aggatcgaat cccaccgcaa aatattgaag ctgaacaagc ggttttaggc tctgtttttc
    10081 ttgacgcaga aacgatcatt gatgccatgg agtacataga gccaaaagac ttttatcgtc
    10141 gtggccatca aattattttt gagacaatga tcgagttaaa tgatcgtaat gaagcgattg
    10201 acgtggttac tgtcaaagac cggctggaac aagccaattt gttagaagat gccggtggtt
    10261 taagttatct ctcagacttg gcattagcag ttccgacagc agcaaatatc gtctactatg
    10321 cgaaaatcgt tgaagaaaaa tcattgctgc gtacattgat ccagacagca acaggaatcg
    10381 ttacgaaagg attcgagcaa ggagaagaag ttcaaacaat tttagatgat gccgaacgca
    10441 gtatcttgga ggtttcggaa aaaagaaatc gaagcggctt tttatctatt gctgaagtac
    10501 tgaactccac gattgaaaat atcgatcgac tagctcaaaa caatgaagaa atcacaggtc
    10561 taccaactgg ctatcaaggt ctggataaaa tgacagcagg tttccagccc gaagaactaa
    10621 tcattcttgc tgcacgtcca gcggtaggta aaacagcgtt tgcattgaat attgctcaaa
    10681 atgtaggaac aaaaacagat cgttctgttg cgatatttag tttggaaatg ggggcagaat
    10741 ctcttgtaaa tcggatgctt tgtgcagaag gttcgattga ggccagccat ttacgtacag
    10801 gtcagctttc ggaagaagaa tggtcgaatc tgatcgttgc gatggggagt ctttctcgag
    10861 caaatatttt tatcgatgat acaccaggga tcaaaatctc tgaaatccgt gcgaaatgtc
    10921 ggaaactagc gcaagaaaaa ggcaatctag gcttgatatt gatcgattat ttgcagttga
    10981 ttgaaggtaa cggtaaagag aaccgtcaac aagaagtatc agatatctct cgccaactga
    11041 aaaaactggc gaaagagctg aaagtacctg tcattgctct ttcacagtta tctcgtggag
    11101 tagaacagcg acaagacaaa cgaccagtgc taagtgatat tcgtgaatcc ggatcaattg
    11161 aacaagatgc ggacatcgta gccttcttgt atcgagatga ttattatgaa agagaaggcg
    11221 gagatgacga tgatggaaac gagccaaaaa atgacaacgt catcgaagtc atcatcgaga
    11281 agaaccgaag cggtgcacga ggaacagttg aactactgtt tatcaaagaa tacaataagt
    11341 tttcgtcgct ttcgccaaga gaagaatttt aaagaagagg ttgtgagcct tgagcaatcc
    11401 cgagagctct cgtaagaaga tgcaaggagt aaccgcagga acgactagag tacttaattt
    11461 aaacagatct ccaaagggat tgtgacaaaa gtcttgtcac agtccctttt tccgaataaa
    11521 cggtgttcaa tcagctgacc cttggtatta gctcagcaaa cgtcaaaata tgaggaacta
    11581 ttttgacgtt tgtttctcta atactcaggt caaaacgctg atcttacgac ctcttttcct
    11641 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatcatca aacatgagaa
    11701 gcagtttttt gaaattgctc cttgtgactc agagttaaac ggcaggctca caacctcttt
    11761 cttaaacggt gttcaaaagc ttgcatctgc ggtaatacgc ccaatcaaac gaaaaatatg
    11821 agaagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacaacct
    11881 ctgattcacg gtgttccaaa agtagcttct tcgaaaaata agtcgaaatt tcacaaaaat
    11941 ttgagaagca attttcgtac attccttctt atttcttgaa gctgaccact tctgttacaa
    12001 cctctttttt aataaccatt ctaaaaagat aaagttcgtt ttttaagcgt ttttataaaa
    12061 taaaaataaa aatcattcga ttttatttga aatatgtatc tattattgat acaatggttc
    12121 aggattaaat aatatagaac gaggtgttca catgtcatct gtagtagtag taggtacgca
    12181 atggggcgat gaagggaaag gaaaaatcac agactttctc agtgaaaatg ctgaagtgat
    12241 tgcacggtac caaggtggcg acaatgcagg gcacacaatt aagtttaacg gtgtgactta
    12301 taaactgcat ttgattcctt ctgggatttt ttataaagac aagatcagtg tgatcggaaa
    12361 cggcgttgta gtcaatccta aatcattggt taaagaatta gcttatttac atgaaaaagg
    12421 tgtagaaaca agtaatttgc gtatttctga ccgcgcgcac gtaatcttgc cttatcatat
    12481 caaattggac caattacaag aagatgctaa gggcgaaaat aagattggta caacgattaa
    12541 aggaatcgga cctgcctata tggataaagc agctcgtgta ggtatccgta ttgcagactt
    12601 attagataaa gaaatttttg aagaacgtct tcgtttgaat ctagatgaca aaaaccgtca
    12661 atttgtaaaa atgtttgatt cagaaccact tgattttgac gatatttttg aagaatatta
    12721 tgaatacgga caacaaatca aacaatacgt cacagatact tccgttatct tgaatgatgc
    12781 tttggatgca ggcaaacgcg tgttgtttga aggcgcacaa ggtgtaatgc tggatatcga
    12841 ccagggaact tatccttttg ttacttcttc taacccagta gccggtgggg taacaatcgg
    12901 aagcggtgta gggccttcaa aaatcaataa agttgtcggc gtctgcaaag cctatacgtc
    12961 acgtgtcggt gatggaccat ttccaactga attatttgat gaaacaggag aacaaatccg
    13021 cgaagtagga cgagaatacg gtacgacaac tggtcgtccg cgtcgtgtag gctggtttga
    13081 ttctgtagtt atgcgccatt ctaaacgagt ttcaggtatt accaacctat ctttaaactc
    13141 gatcgatgta ttaagtggat tggaaacagt aaaaatttgt acggcttatg aattagatgg
    13201 cgaactaatc taccactatc cagcaagctt gaaagagttg aaccgctgca aaccaatcta
    13261 tgaagaattg ccaggctggt cagaagacat tacaggatgc aaaacacttg cagaactgcc
    13321 agaaaacgca cgtaactatg tacgtcgtat ctcagagctt gttggtgtac gtatctcaac
    13381 attttctgtc ggacctgata gaacacaaac aaacatctta gaaagcgtct gggcgcaaat
    13441 ctaaggataa aaacgacgac tgaatcagga cgttcttacc agagcgagtc gaagtgttta
    13501 tctatacgat aataataact tgaacactgt ttattcagaa taagggactg tgacaaaaac
    13561 cgtgtcacag tccctttttc cgaa
//
LOCUS       DAZKNO010000071        13551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000071 DAZKNO010000000
VERSION     DAZKNO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.072"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1020)
                     /locus_tag="ACG8MY_001528"
     CDS             complement(73..1020)
                     /locus_tag="ACG8MY_001528"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0430721.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1210..1479
                     /gene="rpsN"
                     /locus_tag="ACG8MY_001529"
     CDS             1210..1479
                     /gene="rpsN"
                     /locus_tag="ACG8MY_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0430722.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1655..2017)
                     /locus_tag="ACG8MY_001530"
     CDS             complement(1655..2017)
                     /locus_tag="ACG8MY_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0430723.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2028..3149)
                     /gene="alr"
                     /locus_tag="ACG8MY_001531"
     CDS             complement(2028..3149)
                     /gene="alr"
                     /locus_tag="ACG8MY_001531"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0430724.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3165..3515)
                     /gene="acpS"
                     /locus_tag="ACG8MY_001532"
     CDS             complement(3165..3515)
                     /gene="acpS"
                     /locus_tag="ACG8MY_001532"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0430725.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3645..5156)
                     /locus_tag="ACG8MY_001533"
     CDS             complement(3645..5156)
                     /locus_tag="ACG8MY_001533"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0430726.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5478..6830)
                     /gene="murF"
                     /locus_tag="ACG8MY_001534"
     CDS             complement(5478..6830)
                     /gene="murF"
                     /locus_tag="ACG8MY_001534"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0430727.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6983..8059)
                     /locus_tag="ACG8MY_001535"
     CDS             complement(6983..8059)
                     /locus_tag="ACG8MY_001535"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0430728.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8255..10000
                     /locus_tag="ACG8MY_001536"
     CDS             8255..10000
                     /locus_tag="ACG8MY_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0430729.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10280..10717)
                     /locus_tag="ACG8MY_001537"
     CDS             complement(10280..10717)
                     /locus_tag="ACG8MY_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0430730.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10701..12074)
                     /locus_tag="ACG8MY_001538"
     CDS             complement(10701..12074)
                     /locus_tag="ACG8MY_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430731.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12396..12884)
                     /locus_tag="ACG8MY_001539"
     CDS             complement(12396..12884)
                     /locus_tag="ACG8MY_001539"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0430732.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12906..13508)
                     /locus_tag="ACG8MY_001540"
     CDS             complement(12906..13508)
                     /locus_tag="ACG8MY_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0430733.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3876 a         2939 c         2458 g         4278 t
ORIGIN      
        1 aagctaaaac attttttgtc tttgccccta caaaaatgtt tcagcttttt tcgtttttta
       61 ttcctgtatt tcttattttt cgaatgacag gatgattttc ccagcaggag gatgtccttc
      121 aactagctga tgtgcttgga tgacatgctt aagtgaagca gacagattca ttgaaataaa
      181 aacatgaaag gctccttttt ggtaggcttt gattatgcca gcaaaggctt ctgcatctag
      241 atattccttt cttggaacaa acgattcata aaagccttcc tttttttgcc gcaaatctag
      301 atctgggagg tcatttaatg caacatacct gccgcctggt ttcatgatct ggatgccggt
      361 ttctcctttg atacttcctt tcgtcgcatc gattacaagg tctgcctgat cagcaaattg
      421 cagacctgga tttgttttat cataagcagc aaatgctgaa gctcctaatt tttttacctt
      481 ttcctcattt ttactggatg cactggttaa gattcggatt cccttttcat ggagaagctg
      541 gatcagactt gacccgaccg cacctgatgc tcctagtatc attacagtat ctgtcggctg
      601 gatttccgtc aaatggttga ttagattata agccgtaatt cctggcgtga ccattccggc
      661 tgcttcttcc caactcattt tatctggaat ttttgctact tttgctgatg gcaacacgac
      721 ttcttctccg taagtcccgc cgactgcatg gacagctact cgatcaccta catgaacagt
      781 tgtgacatct gaaccaatct ctgtcacaat accagcgcca tcattacctg gtacataagg
      841 gaatttcaat gttcgtatct ctttcatttc tcctaaacgc aaggcaacat cataaggatt
      901 cacactgaat gcttttattt ctacacgaac atgtccagaa ccgacttctc tgggatgagc
      961 atcgatttct tcaaaaacat cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat
     1021 ggtcaccatc tccctttttc tttaagttta cgtttatgtc tttattttga caaaaaaaat
     1081 gtcttttcgt tttggcgcat gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt
     1141 taaattattt atctaaaagc gtaaagatta cgatttacgc taataaaaaa taattggagg
     1201 aatttgttga tggctaaaaa atcaaagatt gccaaagcaa aaaaacagca agaaacgatt
     1261 gaacgctatg ctgctttacg ccaggaatta aaagcgcaaa aagattacga aggcttgcgt
     1321 aaacttcctt tagatgctcg tccagaacga ttgaaaaatc gtgatttaat cgatggacgc
     1381 cctcgcggat acatgcgtaa attcgggatg tcccgtataa aattccgtga attagcacat
     1441 aaaggtttga ttccaggagt aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta
     1501 aaggaggagg gcgcatggac cactccttgg atcatcaaaa aagatacgtt cgcttcttct
     1561 agcagaaatg ctggtactag aagcagacgt atcttttttt acgcacagct ggtccttttt
     1621 tatatgtaaa ggctgcatgg ttacttcgct atatttattt agtaacagga gtcagtgctt
     1681 cgatacccac actaattttc aatgctcggt caacttctgc cataatgggt cgatccaaat
     1741 ggcagacctt ttctttcaat cttgatttat cgatcgttct gatctgctcc aacaggataa
     1801 cggagtctct ttctattcct gttccagtag ctttgatccc aatgtgagtc ggaagcttag
     1861 gtttagccat ttttgccgtt atggccgcaa caatgatcgt cgggctaaaa tggttaccta
     1921 gattattttg aataaccaaa actgggcgtg ttccgccttg ctccgatccc acgactggag
     1981 aaagatcagc aaaataaata tctcctcttt ttaccatagg atcctcctta ttcttgatat
     2041 tctcttggta tccgctgtcc taatccgcaa gccacttcat agtggatcgt acctaattga
     2101 tctgctatat cctgcatagt gatttctttg tctgcatttt tgccgataag cgtcactttt
     2161 gttccgacag gaaattcttg aggcaatcgg atcatcagtt gatccataca tacacgccca
     2221 atcgtttcac agtagttccc ttctaccaat agagaaaaac cctgcatctt tcgtggccag
     2281 ccatctgcat aaccgatcgg tatcgttccg atccactctg cttcaggagt gatatatgtt
     2341 tctccatagc cgattccttc acctgctggg agttttttga cttgaatcaa ttcagaaacc
     2401 agctcaagtg ctggctgcaa tggataaact tccggtagtg cgtgcccaga gggattaagt
     2461 ccgtacattg ctactccata acgaatcata ttgccgattg tttcatcatg ccataaagcc
     2521 gttgcactat tgctgacatg tacatatcga ggaagttcgg gaagtttttt cagcacttct
     2581 ataaagcgtt ctttttgcag attccaatat gtatcatctg cctgatctgc tgtagaaaaa
     2641 tgggtaaata aaccttccca ttctaataca cgactttctt tgatgaatgc agctgcctct
     2701 tttacttctt caggtgtaca aaatccgata cgacccatcc cagtgtctgc ttttagatga
     2761 atcgaaagag gcgtctctag cttacttttc tctaaaacag cagctgctgc ttgcaaccat
     2821 tcccgtgtac ctgcagttac tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa
     2881 ggaaccatat tcaagataag gatgggctga gtaaatccag cttctctcaa ctcgactcct
     2941 tcatctaaat ttgaaacaca aaagcccgaa gctccacctg caactgctgc ctcagctgtt
     3001 tcaatagctc catgaccgta tccgttcgct tttactactg caaacaattc ttttccttgc
     3061 ggaagtcttg caacttcatt tgctacattt tcagttatag cttttttatg tatcactgct
     3121 ttcgttgggc ggtgccaagc gacaaccatc gtttttcctt ctttctagtc ttccaaaata
     3181 atttgtgcaa aagctgtttc atttgtatga gtaatactga cccatacatt gccttcatga
     3241 ggtgaacgcg tcacaacagg ctgaccattt tcatttttta aaatctcgac atcttgaaag
     3301 gtaacttttc cgatcccagt tccccaagct ttagagaaag cctctttgca agcgtaacgc
     3361 ccgcctaaaa attctacttg gcgatgaaaa ggcaaggact ggaatagttt catttcatca
     3421 gaggtcaaga ttcgtgcaat gaattttggt ttttcttcga tcagccgagt gatcctagga
     3481 agttcgactg catcgatccc aattcctttt atcatatgct tttctctttt caataaattt
     3541 agaacactct cattctacca aattataacc attttgccac aaataagtcc acaaataaca
     3601 aaaatgaaag tttctcttaa aaaagagaaa ctttcatttt atgtttaatc gttatttgaa
     3661 cggattacaa atccgcgttt tttatcattg tgacgtttac cgccatcttt ttggctgtta
     3721 ttatttttgc tataaccgcc ttttgatttg tttccacgat aattaccgcc gtcacgacga
     3781 ttacggttgt ttccgccacc acgtttgcca tttttattgt atcctttttt ctgcattggc
     3841 aatgggcgtt ctggtgtgat cttcactggc actgcatcag ctggatcttt tgctgtcgtt
     3901 tttagcaaaa gtgcaactaa atcttgtgca gaatattctt ctaataattt atctgctgtt
     3961 tgcaggtatt tatctagacc attttctgca agttttgttt cgatttgttc aactgcagca
     4021 cctaattgac ctttgaatgc ttctttttcg gttggtggac gtaaagtcgt catccgtttt
     4081 ttcgtcaagt tttcaatcac atgcaagtag tccatttcat ttggtgtaac gaaagtcaca
     4141 gacataccgc cttttccggc acggcctgta cgtccgatac ggtgaacgta gctttctgga
     4201 tcttgaggga tatcatagtt gtacacatga gtgacacctg aaatatctaa tccacgtgca
     4261 gcaacgtctg tcgctacaag gatatctaaa tgaccgctct taaatgaacg caatacgctc
     4321 attcgttttt gttgagacaa atcgccatgg attccttctg cacggtaacc gcgtgcttct
     4381 aatccgcgtg ccaattcgtc tacacggcgt tttgtacgtc cgaagacgat tgttaattca
     4441 ggtgtttgaa catcaaacaa gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg
     4501 acatagtatt gatcgatcaa gtcagctgtc atttctttcg ctttgatctt cacatgatgt
     4561 ggatttttca tgaatttaac gccaatattc ttgatagctg gcggcatcgt tgctgagaaa
     4621 agtaatgttt gtctttgatc aggaacttgt gaaatgattt tttcgatatc ttccaagaag
     4681 cccatattca acatttcatc tgcttcatcc aaaactaatg tttgaacggt tccaagtttc
     4741 aatgtgtggc ggttaatgtg gtcaagcata cgtcctggtg tacccacaac gatatgtgga
     4801 cgatctttca agccacggat ctgacggcca atatctgctc cgccgtaaac ggcttgtaca
     4861 cgtacttttt tgtcgcgccc taaacgatat aattcttctt gtgtttggat agctaattca
     4921 cgtgttggtg cgataactaa accttgtaat tcgtgacgat ctggatcgat tttttctaac
     4981 attggtaatc caaatgcagc tgttttccct gtacctgtct gtgcttgtcc aataacgtct
     5041 tttccagcta atgctagtgg aatcgtttct gcttggatag gcgttgcttc ttcaaaacca
     5101 gcgcgttcga ctgatttcaa caattctggt gataattcta gttctttaaa tttcaaatga
     5161 atcctcctat atggttgtta actgaggatg tagctttttt gctaccccgt cgatttgttc
     5221 ggtcaaagtg tattgtttct ttttgacagt ggccacgcac gtttttatcc attcttagcc
     5281 cctgtggcac caccgaaaaa tcactttgcg aagtttaggc attagaaaag ccaaacgggt
     5341 cgcgttcagc tttatcatta ttatgacatc tggcttcaac aagttgtttc tatggcaaaa
     5401 ccagtccaat ttctcttcat ctttcggcaa taggttattc taacatagtt tttcgttttc
     5461 tgcaagttta cagattttta tttcatttgc tgaagttgtt ctaccacttc aaataagccc
     5521 atgctgttgc tgccttttag cataatacta tctgttgctg caagattttg tttgatagca
     5581 tcaatcaatt tattcttttc tttttcaaaa tagaacacag gtagttgggg gtatttttgg
     5641 ttcaattctt cttttagtgc atgcatttgt tccccataaa ggaaaatcat ggcgaatttc
     5701 tcaggatcga tatgttcact catttgcgca tgcatattta aagactcttt ccctaattcc
     5761 agcatatctg ctaacacagc gattctgcgt ccttttgttg gcagattttt aaaagtatct
     5821 aataccagtc ccatggctgt tggatttgca ttgtagacat cgctcaaaat agctgcacca
     5881 tttcctgccg ttagccattc ggttctgttt tgagtgatag aaacatgggc gagcccttgg
     5941 cgtatctcag gtatggacag tccaaagaag cgcccgatcg tatacgcaat caaagcattc
     6001 gttacattat aacttccagg cagcgggatc gtaaatgttt ctccttctac tacaaaatcg
     6061 gtttgtgctt ttccttttgc tgtcacttcc gcatgaacat cgccatttcc aataccgaat
     6121 gttgtgactg tttgtactaa tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc
     6181 acaacgagta atccgtctga tacaagtccg tcagtgattt ccattttggc tttagcgatt
     6241 ccttctcgcg acccaagatt ttctatgtgt gcttcaccga tcattgtgat tgctgcagct
     6301 tttggttgag ccataagtga aagttccgtg atttcattgg cgtgatccat gcccatttcc
     6361 aaaacgatct tttctgtatc ttggggcatg tgcaaaatcg tgtaaggaag accaatatta
     6421 ttgttgtaat ttccttgcgt cttatatgtt ttgtattttt cagacaagac agacgcggtt
     6481 agatctttcg tggtggtttt accattgctt cctgtaattg caacgacttc tgcgcctact
     6541 ttttccaagt aataggttgc caattgctgc atggctttca aaggatctgc tacaggaata
     6601 actggaaaat tttttggagc ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg
     6661 tttgtgatcg cttgctggat aaattcatgt ccatctcgcg taccagcaag aggaacgaat
     6721 aaacttcctt cggttatctt ccggctgtca aattcgacat tggtaatctt tgtcagtgca
     6781 gagcattcgc tgcccacggc ttgtgcaatt tcttgtattg ttagattcat tgagatctct
     6841 cctaaaaaag gttgtgaaaa aagcgtttgg cctaaacggc gggttcgcat gactctcact
     6901 tccaagtaaa tttctctatt cgtatttaga agctagaatg aacgcggtta cgcaatcact
     6961 ccagcctctt ctttatttct ctttattcat ttttttcaaa aaaagattga cgctgatggt
     7021 atcgattcat tcctaactgg atcagttctt caatcaaatc accgtatttc aagcccatat
     7081 tttcccataa gagtgggtac atactgaact ccgtaaatcc tggcatagag tttaattcat
     7141 tcaggaataa ttcattttta tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac
     7201 ctaacatcgt gtaagctaac ttcgcgtact cttgcgcttt ttgataaact tcttctggca
     7261 cttcggctgg aatctgcatt tcgattttat tattgatata ttttgcttca taatcataga
     7321 atgctacgtc ttttacgact tcaccaggca aagtcgtccg aacgtcttca tttcctaata
     7381 cagcaacttc gatttcgcgc gcttcaattc cttgttcaac gattgctcga gaatcatact
     7441 gataggctgt tgctaaagca ttttgcagct cttctcggtt ttctgctttt gtaatgccga
     7501 cactagaacc catattcgcc ggtttgacaa acatcggata aagcaaagaa ccttcacatt
     7561 gatcaaatac ttttttagga ttttctttcc attgattctt aagtactggt acataaggaa
     7621 cttgcggcac accagcagct tgtaaaatat acttggtcat gattttatcc atggcacatg
     7681 cactggtcaa tacgcctgcg ccgacataag gcatattcaa tgtctctaag aagccttgga
     7741 tcgttccatc ttccccgttt ggcccatgta aaactggaaa aacgatggct ccttcttctt
     7801 tgatttcgcc cggattgatc acttttcctg taaagccttc ctctgtctgt ccacttgggt
     7861 cccatgaaag atgcaagaca tctttgctgg caggtttttc tgttagtaat ggacctttga
     7921 cccattgtcc ttcttttgta ataaatacga gttgaacttg gtaataatta taataaatgg
     7981 catttaaaac tgaaaatgcg gaaagaatgg acacttcatg ctctgcgctg cgtccgccat
     8041 atagtaaagt aatcttcaaa agattgtcct cctgtattct aaatcgttca gtgatttact
     8101 ctcatatttt agcacaaaac aaaaataaac gaataactta tttataaaaa aacgagccag
     8161 cttctaaaag aacgagcgtc atttattttt tgtcagctag attatttgtc ccgtttttta
     8221 taaaaagtta tgataaagaa aaggagggat gatgatggcg caaacaaatc cacgcagccg
     8281 cttggcagaa atcattcgag tgttcatcaa ttacaatgta gtgccaaact ttgtacagca
     8341 aaaaaatccc gaacaggtaa aaaaagcctt tgaagaacta ggccccacct tcatcaagat
     8401 aggacagatg ctttctgttc gagaagattt gctttcttct gccttcactc aaacattcaa
     8461 aacattacaa gatagtgtac ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac
     8521 agaactttct ctttcattat cagatatatt cgatgatttt tcaaaatcgc cttttgcctc
     8581 tgcttctatg gggcaggctc atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa
     8641 gatccaacac cctaacatcg ccgaagagat ccgacttgat cttcaactat ttgaacgagc
     8701 gatccctttg atcaaataca ttcctgaaac tagcgtagtt gatctaaaag gggtactcca
     8761 agaagtaaaa cgttccttga tcaatgaaat ggacttctta aaagaaagtc aaaatggcga
     8821 acaattctat caaaagaaca atggctggaa agagatccgc agccccaaga tttatgacgc
     8881 tttttgttcg aaaaaagtga tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt
     8941 aatgaacgcc gaaaacaaaa ctgaaacttt tatcactgga attcaaaaca agcagctgaa
     9001 acaagaagtt gcaaagcttt tagttgagaa ctttatgaaa caggtgtttg atgatggttt
     9061 tttccatgct gatcctcatc cagggaatct gctattccat gtactaacaa aagaagaaca
     9121 gacacaagct agccgaaaaa ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt
     9181 tcgagcctcc acttctgcag aagaccctgt tgcgccatat accatcaact acatcgattt
     9241 tggtatgatg gggcatcttt ctgcaggact taggcaaaaa ctcacacaag ccgttttagc
     9301 gctgtatacg aaagatgctt atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga
     9361 aggctcgttt gatgaaagtc ggttccacca agaactgaca agttttcttg aacagtatta
     9421 tgattctcct attgatgaaa tcaacttgca agaagtattc acacatgtcg tgacgatttg
     9481 tcatcaaaat aatctgcagt tcgatcgtga tatcacgctt ttgctgaaag catttgggac
     9541 attagaaggg gtgatccgcg tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc
     9601 ttttgctcaa cattattttc tcactcatct ggatgtagaa gatacattga aacaaagcgg
     9661 attagactta ttggaaggga tgaaggctgc gcctaagatc cctcaacagc tccaccatct
     9721 attagaaatg tggacttcgg gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa
     9781 attattgagc aggatcgaaa gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc
     9841 attgatcgta gggtcctccc tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt
     9901 ttccttatta gggatattta cgtatgctat tgccgcattc gtcattattt ttttggcaat
     9961 cgatgcattg atccaaatgt ataagaagag gaaaaagtag aaaaaggttg tgacagaaag
    10021 aatcagcttc aagaaataag aaggggtttt acataaattg tttctcaaat ttttgtgaaa
    10081 attcggctta tttttcgaag aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa
    10141 gctgttctgc acccagtaat aagaacaatc aaaaagggaa catggaacac atttatcctg
    10201 tttccctgtt ccctttttga tgtattcgag cataaataaa ataccttcgt tacctactat
    10261 cttctgtact tggtaaacct tagtcaattg ccttttttaa cgcttgacga aaagacatat
    10321 gatctttctc cgtaaagggt tttggaccct ttgtccttat gccagctttt cttaactttc
    10381 cactatgata acgttgttct agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag
    10441 agtgatggaa cactcgcaca cccttcggca atacaagtga agctaaacca taatcttgcg
    10501 tgatcaaaaa gtcattcttc ttaaccatac cgacgatttt ataatccgca ctatcagctc
    10561 cgcggtctac gtagacgatt tggacatgat tagcgtattc tttggtcgta taatggtcca
    10621 cactggtcac gataaccacg tcaagaccat aggattctcc taattttatg acatcttctt
    10681 ttactggaga accatctcca tcaatgaata gcctcattct ttttcgcctt cttccataaa
    10741 tgataaacag ttactaatcc gctcagtaaa agaccaacat tgatccattt tgccaagata
    10801 agcagttgta cactttctat cggcactgat tcatggttca gcgtgatcaa taatagatgg
    10861 attggctctc ccatttcttt tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg
    10921 atcacactcc ctttattttg agtagtatac ttgacaacga tccggcgatc cattggaata
    10981 gctgtatcag tttgataata ttccttattt agccagctgc ctgttactac gtgccagaaa
    11041 ctaagcagaa agacacctat cgcaaaacca tttggaatca agaggaccaa taaagaagaa
    11101 ctcagtccaa gcaattgcat agccaatgga gaccatgtta aacgtttcaa aatccatggt
    11161 ccgactattt ttgctgcaat caagcctgct gcataaggca aatacataaa aatcgaaagt
    11221 ttttctaatc cgtacagtat gccaacataa aaactgctaa ataaaaaaag gaaattccct
    11281 agcattccat ttagaaaagt aaacagattt agccagcttg gcaacttcca ttccttcttc
    11341 tggtgtccta aaacaaacac gaacaagatg aacagcagca agaaaccata aatagcaaga
    11401 tcaaaaagcc tttcatcgaa taacaatcgg gcatttctca aaagaaacaa taagccaaag
    11461 aatatcaaaa ataaaacaag ctctcgcgta gctaccagat ttcttttgat gtctttgaaa
    11521 tccagttcgt aacgcggata atgactaacc gtatggtagg ccatgacgta aaaaagtgta
    11581 taaatagtaa agcttgctgc aatctgagtc ggtgcaggca actcaatcgc ttgatacagg
    11641 agaaatagcc agatagctgc aaataagtac tttttcccat ccatattgat aaaaccttgc
    11701 gtcttttcgt gaaaattgac cgtggtatta gcaggtacaa gccatgctgc actcagccct
    11761 agcaaaacac ttgaaaaata aaataaagga aaataaaata cgcctaatat tccaagcaat
    11821 gaaccagcac caccaatcag cagagcgatc atcagcaacg tgtaactatc tagaccaaat
    11881 cgtagactgt tgactaaaaa tagtccagtc atccgaaagg cataaaacag cgtaaatggc
    11941 aaaatcgtca gccaattatg cgagtctgtt agtcccaaaa ataaaagata aggaataaaa
    12001 ataaccgtgt tacttaagat aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg
    12061 agtttttcga acatgacaat ggctccttaa acagtcttat aactaatctg ctcttcgatt
    12121 ttagcattaa aggggaaata aaccaatttt acagaatata ttcttgtaaa aaaaaataca
    12181 gaaagaagca taagcttctt tctgcatttt tacggtactt gattactcaa gcagacaagc
    12241 ccttctccac aaaagtcttg cccgacaccc ctgcatccgg caggtgataa agatagaaat
    12301 caattatcct gtgcctccaa aatcaattag gagctctgta aaccagagtc cttctcgacc
    12361 cgtcgcataa atttacttta acacagttcc tatgtttatg cgagagcttt tttcatttta
    12421 taatcgacca tttgaaatcc ttgaccgata tctgaaataa gttcttctgt aatctcaaat
    12481 ccttcatgct tataaacctc gattgcttga tgattcccct gattcactcg caaaaagatc
    12541 tctctttcct ttgtttgcgc agcaagttct tcataccagc gaaaaactga agaagtcaat
    12601 ccttgaccac gtactttatt tagcaaatat aatttactta aataaatttt tccatttatc
    12661 tcttcataag cagtgtaacc gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca
    12721 ccttctgtca gttctttttg aatctgtttt tttgactgat aagttttcag catgtaatcc
    12781 acttgttttt gaccaatgat cggcacaaaa gcttccggcc atacttcttg tatgactgcc
    12841 atcagctcat ccagttcatt ttctgttact tttatttttt ccatacaccc acgtcctttt
    12901 ttacattata aatgaccttg cgcttgatgg aataaaaaga gcatgatcat gctatacaca
    12961 cccaccgacc atggtttcaa gaaaatcaca atctgcataa attccctaaa agataatttc
    13021 gtcaaaccag ctactagaca gatcaagtcg tctggcgcaa agggaagcag caaggtaaca
    13081 actagtactt ttttaatatc tttcgttttt cttgtcagca attcatcaaa tttctgatac
    13141 ttttttggcg ataaaataag ctggacaaag gctacgccat agtaacggac aagcaaaaat
    13201 ccgattgctt ccccaatcac taggccaata caactatata aaagcccaaa tccattgcca
    13261 aacatcagca tgcccgctac tgaggagatc cctcccggca aaataggaat gatcacttgg
    13321 atcacttgca acagaacaaa tatcatcact gcatacttgc caaattgctg gataaacgct
    13381 tgcaaagacg tgctcgatcg aaaaatccca tgatgataag cataaagaat caacacaaag
    13441 aaaaaaaaca gaccaataat gggcatgatc tgaagacatt tttgtaaaac tgatcctttt
    13501 ttagacatct atattcccca catctttttt catttattaa aactgcctcc t
//
LOCUS       DAZKNO010000072        13181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000072 DAZKNO010000000
VERSION     DAZKNO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.073"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..276
                     /locus_tag="ACG8MY_001541"
     CDS             <1..276
                     /locus_tag="ACG8MY_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430734.1"
                     /translation="SELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVRTRSAKG
                     IYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(373..807)
                     /locus_tag="ACG8MY_001542"
     CDS             complement(373..807)
                     /locus_tag="ACG8MY_001542"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0430735.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1039..1749)
                     /locus_tag="ACG8MY_001543"
     CDS             complement(1039..1749)
                     /locus_tag="ACG8MY_001543"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0430736.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1739..2599)
                     /locus_tag="ACG8MY_001544"
     CDS             complement(1739..2599)
                     /locus_tag="ACG8MY_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0430737.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMLIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2604..3242)
                     /locus_tag="ACG8MY_001545"
     CDS             complement(2604..3242)
                     /locus_tag="ACG8MY_001545"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0430738.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3257..3556)
                     /locus_tag="ACG8MY_001546"
     CDS             complement(3257..3556)
                     /locus_tag="ACG8MY_001546"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0430739.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3599..5083)
                     /locus_tag="ACG8MY_001547"
     CDS             complement(3599..5083)
                     /locus_tag="ACG8MY_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0430740.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5103..5564)
                     /locus_tag="ACG8MY_001548"
     CDS             complement(5103..5564)
                     /locus_tag="ACG8MY_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0430741.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5582..6649)
                     /gene="ulaG"
                     /locus_tag="ACG8MY_001549"
     CDS             complement(5582..6649)
                     /gene="ulaG"
                     /locus_tag="ACG8MY_001549"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0430742.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6867..7637
                     /locus_tag="ACG8MY_001550"
     CDS             6867..7637
                     /locus_tag="ACG8MY_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0430743.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7910..8737)
                     /locus_tag="ACG8MY_001551"
     CDS             complement(7910..8737)
                     /locus_tag="ACG8MY_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430744.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9305..10540
                     /locus_tag="ACG8MY_001552"
     CDS             9305..10540
                     /locus_tag="ACG8MY_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0430745.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(11539..11799)
                     /locus_tag="ACG8MY_001553"
     CDS             complement(11539..11799)
                     /locus_tag="ACG8MY_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0430746.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(11826..13127)
                     /locus_tag="ACG8MY_001554"
     CDS             complement(11826..13127)
                     /locus_tag="ACG8MY_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0430747.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4179 a         2755 c         2072 g         4175 t
ORIGIN      
        1 agtgaactac ttaagcaatt tccacaagaa gaacatttca tgtataaaga agcaatttcc
       61 cacaaagtga cagagtattt ggagcataat ttttttcgta taaagccagt gcgtactcgt
      121 tcggcaaaag gaatatacga gatgaaagtc aaaattaata aaaaaaatta tcgcatagca
      181 tttgctttaa aaaaaaatca tgtgttcatt ttttatatta gttcgaattt gcaaaaaatt
      241 gtatttgata aagaggtgag taaaaaaata aaatagctga gcattggagg atagattgag
      301 tgatactaag tctatcctct tgtttttcta aagaactaag ctagaatttt ttcttttgta
      361 cgttattata tgttatttgt cattaccaaa aagcaccttt tttcttctag tatctatgac
      421 ataagaaata ccacccattt cctttacttg aacaaatcct actgattcat aaaatttttt
      481 tgttttctca gtatcatctg tcaataattc gatttggact ggtccataac tagtgatctt
      541 ctccaaagct tcattcaaaa gcactttgcc tattccttgg cgctgatacc tcggatgaat
      601 gaggatatct tgaataaatg cgatcgtcgc aaaatcagtg atccaacgaa cgattccgat
      661 aatcttccct tccccattac gctttatcag agattcagat tgttcaaatg ctctagctaa
      721 ccttgcagtg tcttttgtgt aattcgtcca accaacgctc ttatataatt cagaaatctc
      781 atcgatcata agggaatcaa tattcatttt tttcttcctt tcttatacaa ttctccctct
      841 aataatacag ttctttgact atttaatcta tcacatataa ctctcctgta aagattctct
      901 cgattcctta aaattcctct ttccttttag aatgcatact ttttgataaa taaaaaggtt
      961 gtgataaatt ttcttcacaa cctctgtttc tctctatcaa gtgttatctt ttaattttct
     1021 acccttaccc tagcatctct attgcccgta gtatgcatac tttccatgtt tgcgaaaata
     1081 atgtttatct agtaaatatt gcgggatttc acatatattg gggttgatca gctcgttttc
     1141 tttggccatc agacaaattt catctaagat caaactgttt tctactgctt tcataggtga
     1201 atccccccat gtgaacggtc catgtccata gacaagtact ccagggactt cgtttggatc
     1261 taattttctt tccttaaacg tctcgacaat cacattccct gtatgaactt cgtaagcttc
     1321 tcttacttct tctttcgtta attggcgtgt acaaggaacc ttaccataaa aagcatcagc
     1381 atgagtcgtg ccataagctg gcaaatcgcg tcctgcttgc gcccacatga cggagtgcgt
     1441 ggaatgagta tgagtaatag cagttatatc ttcaaaagtt tgatacaaaa caacatgtgt
     1501 ggggagatct gaagatggtt ttaagctatc ttcttctatt atatttcccg acaaatctgt
     1561 taccaccatt tgatctgctt gcatgcattc atatctgaca ccgcttggct taatgacgat
     1621 gactcctagc tcacggtcca cttcactcac attgccccaa gtcaatttga ctaatccgta
     1681 tcgcggcaat gccagatttg cttcaaggac acgttgcttc attgctgcaa ttttttcttc
     1741 acaagacatc atagccagcc tccttcaatt tagaacttaa gtagtctttt gcctgctgga
     1801 tttctcttcg aaagtcttgg cttttttcac tccacatttc aatcagaaat gttccatcat
     1861 aatctagacg tttcaaatgt cttaataatc caagaaaatc aacacaaccg ctaccaaaag
     1921 gaacatcacg aaactggcct gaatattctt ttgttacagg gtaagtatct tttaagtgga
     1981 tagcagtgat ataatcgatc cctttctcaa gttctcgagc cggattattt tccggccatg
     2041 cggacaaatt gcctaggtct ggatatactt gtagaaaagg tgaatgaatc tgttctttga
     2101 tttctaaaaa ttttgaaatg gagttcataa atggatcatc catgatttcc acggaaagaa
     2161 tgatgccata tttagaagct tcctttacgc tttcttttat tccttcgata aagtcttctc
     2221 tggaattcat tgatttctct tcataataaa catcgtatcc agctatttgg attactttga
     2281 cgcttagatt atgcgccaaa tggattgctt ttttcattag ttgtttcgct gcttttttct
     2341 tttctggatc agcagaacca aacggaaatc gccgatgcgc acttaaacaa atcgaatgga
     2401 tacgtacacc ggagctgaac atctcttctc tcagctcagc tatcttttct tcagaccaat
     2461 ctaatcgtgc taaccgctca tccgtctcat caatcaacat ctcgataaaa tcgaagtcca
     2521 tttcttttac aagggaaaaa cgttctttcc atgaaatatc tttgggtaac gctttttcat
     2581 aaatcccaat ttgagccatt tccttacccc cagatacgaa tgatctcatc tttgaagttt
     2641 tgtgcggcct tcatcggatc atttgctgct gtaataccac gaccagtgat aaaggtatac
     2701 acatcgatcc cttcaaataa gtgtaatgtg tgtggattta atccaccagt tacagaaaca
     2761 ttaaagccta gttcaatcag ttttttgatc ttcgataaat ctttttcacc ccaagtttca
     2821 ccagataaca gcgcatcacg gctttgatgg tagattacct gccggatacc gatgttatgc
     2881 caatccatgg cttgttcaaa agtccaattg ccgtagagtt ctacttgcaa ttcacctatt
     2941 tctttttgtg ctgcctccat agttggaatc gttgcacaac aaatgactgt catccagtct
     3001 gctcctgctt ttgcaacatt actcgccact gttcctccag cgtctgcaca tttcgtatca
     3061 gcgactagtt ttttatctgg gaacatactg cgaatacagc gaatcgcttt ttgtccttcc
     3121 tgtaagcaaa gaattgtgcc cacttcaata atatctacta catcaccaac tttcataaca
     3181 tctcctaatg catgttctaa gtcattgtga tctaacgcaa tttgcaaatt cggcctgctc
     3241 atcttagttc cttcctttac ttgttttttt ggtaccactt tgtttcagca aatttttcac
     3301 tgatttcttt gactgacatc acatttttga taccgataac tgtaactcct tttttctctg
     3361 cctgagtaaa catattcaaa aaattgactg gcgtaaaaac tacatcgtat tggccagctg
     3421 aactttttcc ctctgaaatg gcacaatgat ggattttggt aattggtacg tttagttctt
     3481 tcattgcttt ttccacactt ctcatcatca tcaagcttgt tcctgatcca tttgcacacg
     3541 atactaaaat tctcatctta atccctccat tatgagtgtt gtctaatcag attttctttt
     3601 atactctctg cttttctgca tatgtgtcat agtcttctac aatcaaaaaa taaccatcag
     3661 gatttttccg atattgtaac tgcggaatag ccaataaacc aatgacaact acggcaattc
     3721 cagcataacc taggactttc atgattactg taaacaatgg ccaagctgtt gcccagtcga
     3781 acatcccgat ataaccaccg tatgttgaca tcccaatcca tgtagcgaat aatgctgaac
     3841 cgcctacttg tatgattcct gaaagaaacg ggaacaggca agcggctttg aatccacctc
     3901 ggttattggc ataaactgct ataacggcat tatcaaagaa caacggaatg aaaccggcta
     3961 tgacgatcgt tggtgatttc aataaaatca gtaaaccaat catcaaaaac tgtcccaaag
     4021 caccaaacaa gaacccaatc gttaccgcat ttggcgaacc aaagccaaaa gtagcagcta
     4081 cgtcaattcc tggaacagct cctggtaaaa tcgtgtttga gattccttgg aaagattgag
     4141 ttagttcatc gacgaatgtc cgaacaccta attgcaagat cgccagatat accgcaaaat
     4201 ttaaagaagt cgtcataatg tagaataaga aactctgccc ttcttgcatg aattgcgctt
     4261 gaatcaaata atctttgcct agtacaatca gaatgatccc aaagaaaaat agcatgagta
     4321 acgaagtagc aaccatattc tcattgaaaa tcgacaagaa tcctggtaat ttgatatctt
     4381 caattttttt gtcttgcttg attgatctat ttttcttttc atcccgcttt ttcatccact
     4441 cagctaattt tgcaaatata aagattccga acatttgctg atgtgccact gcaaatccgg
     4501 caccttctgt caattcctgc gtgattccta ctgttaagtt agaagctact gcccaataac
     4561 aacctaaaat cagtcccata aaaatcaata cttgtatcct acctagatca ggaaagcaaa
     4621 acagtaaaat ccaaaaagcg gtagctgctt gttgaacctg aacgtttccg gtagtgaaaa
     4681 cggaacgcag tttcgtatat ttctgaaaac ggactaataa gatatttaaa acaaatgcaa
     4741 tcagtaataa aatcatcaca tctccaaagg tacggccaaa tgtctccatt aatccagcat
     4801 ccacagcatt ttgtccaaaa taaggatctg taaccatggc atccaaattg aaccgttcct
     4861 ttagtccaac caagattggc cgaaaattat tgaccaaacc accagagcca acagtcaaaa
     4921 taaagtaacc gaccgttgcc tttaagaatc cagcgatgca ttcatgaagc gggcgtttca
     4981 gcaaaatata gcctaataac acgataaaac caatcaggta tgctggctgc gtcaagatat
     5041 ttgttgcaaa atacgtccaa atgcttaaca aaaattcccc catctttttt ctcctctatc
     5101 aattaattat attctgccat cacaacttgg taatcatcga tggagttcgt agagatgaga
     5161 cgctcaacca atccatctgt cattaataat tcggataatt tttggatatt ttctagatgt
     5221 tcttctggat cttttgctgc gagtgtaaaa aacaaactag ccgttttctc ctcattctcc
     5281 ccttcacttt caaaacggat tggctgtttc aacttggtaa atgaaatagc tgttccaaaa
     5341 acaccatcac ttttttctga agaatgcggc attgctactt gaggtgcgat cacaatataa
     5401 ggaccgtatt tttctacaca atggatgatc tcctccacat acgtttcatc aataatttcc
     5461 ttatcgatca atacaagaca gctttcggcg atagcttcac gccaatccgt gatttcttta
     5521 tctgggtaat gaatcaattg atggtcaaaa aagtatttca gcaagaaact ccccttcttt
     5581 cttataaaaa cgacggaaac ggtgtatcat tttcaatcga gaagacatct tcaaatcctc
     5641 gataaaaatt aaactcaaga tcatctttgt ttgtcggata gatgaatttc ccgcctactt
     5701 gccagatata aggtttaaat ccatacttca ggcgatcctt tttgaatttc caaatttcag
     5761 tgatctcttt tggatcagcc ataaaattag accaaatatc gtagtggaca ggaatcacta
     5821 cttttgcatt caatgactcc gccatgcgca gcatatcaac agaagtaact ttgtctgtta
     5881 tccctcgtgg gttttcacca taggccccta aacagacatc gattttgtgt tcattaccat
     5941 gtttggcaaa taaattggag taatgtgaat ctcctgcatg ataaagattc cctcctgatg
     6001 tttcgaataa atagtttact gcaatttgat ccatctcctg aggcattttt ccttttaata
     6061 cctcttctgg atcttcacaa gtgaccaacg ctgtccgatc aaacgcttct aaagctacta
     6121 cattgatgtc tttgattttg actgcatcac ctggctttac tgtaatcgtt ttttcttcag
     6181 gaattcccca tttttgccac gtgcgaacca cttcttttgg cccaatgaat gttgcttcag
     6241 gacaattttg atgaactgct gcggctgtat ggatatctag atgatcggaa tggatatgtg
     6301 taacgattaa agcgtctaca ttttttatcg caaaaggatc gattacgaaa ggctgagtac
     6361 gtaaattagg ctgcatattt tgaacgccac tcatacgcat catctgatgg ccttttttca
     6421 tcatgccatt cccatgacta cgttttcccg tcccacacca tagatcacat aaaatattcg
     6481 ttccttcatg acttttcagc caaattcccg tacagcccag ccaccacata gcaaaagttc
     6541 caggatccac tacttctgtt tcgatttctt cattcaagta tgtgccccac tcaggaaatg
     6601 tttctactaa ccacttttct tttgttactt cattgacagt tttactcatt tgcttgctcc
     6661 ccccatatcg ttgttccaac ttacaagtaa atattaatgt gtatcagcat ccatgaaaac
     6721 ccttttatat gaccgtttgt tttttatatg attttctttc agtaatcaat gattgtttca
     6781 gtataataaa gacagatatg aatgtttaaa tgaacaacta gtcaaataaa tccatggatc
     6841 aagatgtccc tcaggaggca aaagtcatga aaaactcact tgtcaatatt gaaaaacgtc
     6901 aaaaagaaat tttggaactg cttcagcaga ctgaacaact atcaactttc gaattagctg
     6961 aatcccttga tgtatctatt agtacgatac gaagagattt aaatttatta gaagaaaaaa
     7021 acgagatcat acgcaattac ggttattgtt cttataatta caaaaaccag actgattttg
     7081 atcaatctgg tcctgaaagg attaaacaag cgattgccag agaagctagt aaatacatta
     7141 acgattatga tacgctgttt atcaattcta gctcgactgc gttgaaaaca ctggattttc
     7201 ttcaatctaa ccatttgaca gtcattacaa acaacgtgaa aatcaattac tctccgcaca
     7261 aagataattg tagttatatt ttaactggtg gagagatgcg ctttccaaaa gaagcactag
     7321 tgggcgacat cgcattaaat acgatttctt cagtcaatgc agacatttgt atcattggct
     7381 gtagcggagt cgatgtagta catggggtca caacaaagat attaaatgaa tcaacgatca
     7441 acgaaatgat ggtcaacaag acaatcaaat gtaagatttt agtcgctgat catcgaaaaa
     7501 tcggactgac atcaaagttc aagattgctg atatttcagt ctttgattat ttgatcactg
     7561 ataaattttg ttctgaaaaa gtattagagg agatcagaaa tacggggata aaaaccattc
     7621 aagtagattc attttaagaa ccaggacgta aatatgtaaa taggattatc aaaaaaatga
     7681 ataacaaatt tatcgataaa tgaaaaacac gctacgctca aattgataaa ggaatcccaa
     7741 gatgtatcca tccaatcgat accatcataa tctattgctt cttttataac cgttatatgt
     7801 tgcctaaata ggaatcaaac aatatcgaaa aaaggaggta tgacaaaagt cacacctcct
     7861 tttttcgaat aaacggtatt caaatcagaa cactgatttc acaaccactt tatttcaggt
     7921 cgttttcaat cacctttacc gcgtaatcat ttccgttgaa acgcgctatt ttttttgcgt
     7981 ccatatatct ttctaaataa tcatcttctc ctaattgctt atggcaagaa gcaatttcat
     8041 aaagcaagta ttccaatgca taagtcgatt tatgtttctt attatactca atacccttct
     8101 cacataattg gattgcttta tcataatcct cgattttacg gtaataggtg gcgatatcat
     8161 aatatacaat gcagaccatc agattatcaa aatcagtctc actcaatatt ttaagtgtac
     8221 gctccacata aactttcgca aaatctaatt ctccttttga caagtagatc attgccacgt
     8281 ttgcagctgc ttgtaagtga tggatatctg tttcggtcgt ttgttccaac acttgattaa
     8341 aataaaataa tgctgcatct ggttttttcg cataaagatg atcaagcagt ccttgataaa
     8401 ataaaatttg atttaaaaga tcagaattac ttactttttc ctgatcgatc gtttgtaaaa
     8461 ttttataaga ttcttggtat tttgatacag aattcaatga ttgaatctca tttatttttt
     8521 tcctgatttt gtcttcttct gtttctacta atatatcgga gatctcgaca ttcagctttt
     8581 tgcaaagcgc acttaagata ttgatgcctg gcgacatatt cttgttttcg atattgctga
     8641 cagttgcttg ttcacaaatg ccttctgcta attgtacttg tgttaacccc tttttctttc
     8701 ggtattcttt tagtaatgct ccatctactt taatcaaaac aatttccttc cctttaaaaa
     8761 attatttctc ctttatatca tacacaaaaa aaagttctaa acaaccaaaa atgtttagaa
     8821 cagaataaaa attttaattt taaaataata ttcaaattaa acaactggcg tatctgctcc
     8881 ttgatttctt tttgtgttat ctgaataatt atcgtttaca gttataacac atagtgaaaa
     8941 ttgtcatggc gagaaagtct aagaatattt ttaaacataa ctatttcttt cttacgtgtg
     9001 tataatgacg acagcacacc tatcaattaa tataattatt ttattttttt atgttctcat
     9061 gtttattttt aatgacacaa cttctcgtat ttatctggca caacatataa atcgatcaaa
     9121 aaattgttgc gtgttttttt gaaaattgga gtttcaactt gattacaaaa tttaaattta
     9181 gaagttgtga aaacagcgag agacagttta atattcaaaa tatacatagg ctactcgaac
     9241 gctgtgtttt ttagtatact agtaagaaac gaagggatag actcattcca ataaggagga
     9301 tttcatgaag acaattcttt ttgctcctgc agtattcaat ttagctgaga caacacgaat
     9361 gattgaggtt gccaaagcta tgaaagaccg ttattactgt gtattctttg gcttttctaa
     9421 aacttttgct cctttgatca cagaaagtgg ctttgagtat catttactag cgccgatcct
     9481 tacgaaaaaa caagaagaac aaatcataaa atttgaccag ttgcgtacac ttaaaaaccc
     9541 ttttaccaaa gaaatggttg cctctcgtat cactagtgaa cagagtctga ttgctaagtt
     9601 tgagcctcga ctgattgtga caggttcgaa tgtgacaatt tttttatcgg cccgcaataa
     9661 aaagattcct ttagtatatg ttaaacccta tgctttgagt gaaccttttt ttcaatcaga
     9721 gcatgcgctg atgccccacg agttacgttt cttcggccct ttaagcaatc ccctttggca
     9781 ctggacaaaa aagcgtatag taaatacccg atggatcccc aatgccttca aacacgctgc
     9841 acaaaaagaa atgcttcctt taccaaaaac aagtttacaa atgatggatg ctgatctaaa
     9901 tctcatcaca acgcctaaac tatttttgga agaagaacag ctgccggaaa attatcgaat
     9961 cgttggtcca atttttgcta agctcagtac ttccttgcct gaagaagtgc taacttttat
    10021 acagcgaaag caacaagaaa gaaaacgtat tatctatttc gcaatgggta gttcaggcaa
    10081 tccgcgtatg ttacgcaaaa ttttgttctt cttacaaaaa caaccggatc tagcggttat
    10141 tgcacctatt gactatctgc ttgatgacat tcaatctgac tcagaagatc ttttaataac
    10201 agaatatctg tctagtcatc ttttgcatga attcattgat ttttctttta tccacggcgg
    10261 ggaagggacc gtccaaactg cctgtgaatc cggcaaaccg tttatcggtg tggggatgca
    10321 atttgagcaa agctgtaata ttgactattg tgtaaaatac ggaaacgcaa tcgccctcac
    10381 aaaaccagtg actgaaagaa aactgcagat tgctctgcgc gaagtttctt cacccaagat
    10441 tcgtcagcag gcacgcacgc tgcaagccgt ttttccaata aatggtccac aacaagcaat
    10501 tcaagaaatc gaaaccttcc tgagcaacca cccaatatag tttcaagtag aaaaagcttt
    10561 tttaatagag actcatcagt aatttcgcta ttcgagaccc agacagtgaa aatacaaaaa
    10621 aagtaaatag tggattgaaa agttagagtt acttaaatta cggtgtctaa aaaacacaaa
    10681 atcatcctta aaataaaaga tctgacgcta tccaaatgag cataggtagc gtctgccatt
    10741 tattcttagt caaattcttt gacacctttc tctggttgga tcatctcaac gtttcttcaa
    10801 aagaaagacc ataataatta ggcaaataca ttttcttaaa atgtgtcata tagatctaga
    10861 agcatcgctt tgtagagatt tgtgccgcta gtcccctcaa caattactaa atcaaatgta
    10921 ggatttacca ctttatcttt gataatcaac ctccctcttc tgttgagaca atgatctcgc
    10981 aatccgatgc atactcctat ataaactgcc agcaatcccc acatattgag tagatcatgt
    11041 cagaatgttc tatgtcgtca gcaacgatac actaaaagtc acgttcacca tgactgattg
    11101 ctattcccag cacaattatt tctatacacg tttcatgatt tgaataaaca ctcacatcat
    11161 acatattacc attcatacac catactgcag cccctactat atggttgtaa gctcccaaat
    11221 ctatatttga cttgatcact ttggtagtag atctactaat tctaaatcca ttgctgataa
    11281 aatttccatt ttttcctcct tctttctgcc aaaacagtta aaacaataat aatctttatg
    11341 taaaaaaggg ctgtgacaaa agccttgtca cagtcccata ttccgaataa acggtggtca
    11401 aaagcttgca tctgcggtaa taagttcaac caaacgaaaa ataagccaac atttcacaaa
    11461 aatttaagaa gcaattttcg taaatttctt cttatttctt gaagctaacc acttctgtta
    11521 caaccacttt tagaacattt aaagcttcga tgtgacaaac catctatgct gctcgatcat
    11581 aaaattgata gcaaaaattc cacaaagtac atatgtcccc catgaaaaca aaacattcat
    11641 atgcggtaca atgatctttg gaagaatcac gatcaaaaat gatgccagtg cccaaatcca
    11701 tcgattgatt tgaatatgat atgtagctag catattcaat ataaggtgac tagccgttaa
    11761 taaaaagata aaaacaaaaa ataaaatcag ccaatacatt gtttcccacc ttctaaaaac
    11821 caaacttaga cagtctggac ttcgttttct ccacgacttt gttcatctga cagcatcttt
    11881 ttatcataag cttttacaaa tggataatag ataataaatg ctatcaatgt gttgatcatg
    11941 atcaagataa caacacgcca gtcatttcct gctgaaagat aaccgccgat tggagctggt
    12001 aatgtccatg ttggcaaaac agtaaatcta ttgaccattt ctaatttgac tgcaaaataa
    12061 ctgataatag tcaaaactaa aggaactaag ttgaacggga tgaacatgta aggattcaac
    12121 atcataggag ctccaaaaat tatgggctca ttgatattga agataccagg aataatagaa
    12181 aaacgaccaa cctctttcaa gaattttgat ttacagaaga atataaacaa aacagatacc
    12241 acgatcgttg ctcctgcacc accaattgtt accgtccatt gataaaactg ttcaggagcg
    12301 atatagggta attgggtggc agctgtacca tctgccaatg cctttgcatt ctcatccaac
    12361 atgatcagcc acattggacg aacaacagcc cctacaatac tcatgccatg gataccaaat
    12421 gaccacaaca aatggatcaa aaacattggt agtaaaacac caagcaaatt attccctaaa
    12481 atattggtta aaggctcgaa taacgataat aaggcagcgt ttacatcaaa gcccgcgacg
    12541 cttcgaatca gccaagtaat tgttaaaatg actgcacctg gaatcaatgc ctcaaaacta
    12601 cgggcaacag caggtggcac ttgttctggc attttgatag taatatttcg atttttaaag
    12661 aaacgatata cttccactgc aaaaatcata gataagatcc ccgcaaacat cccagatgct
    12721 cccaaagaac tcataggtaa gacccagcca agaggtgcct cttcaggagt taaaacgttt
    12781 acaggaactt gtaacataaa gaacgttcct aaagataata taccacctgt cacactatct
    12841 aatccatatg atttagctag atccgaaccc ataacaaaag ttgcataaat agccatcaat
    12901 cccatcgtca tcctaaaggg taaaacaatc gttgccgaat aaggggcaat aaattcattc
    12961 catccaggaa acggtaaatt gataaagacc ataaaaaatg tcccaatcaa aatcaatgac
    13021 aatgttgaaa caaccccatt acgaattgct aataaatgac gctgtgtacc aatttttgcc
    13081 attggtggga caagtttctc ttcgacccat ttcatcagct tttccactaa aaccactcct
    13141 tttcaataaa atatgaacca ttcattgcat gacaacgcaa a
//
LOCUS       DAZKNO010000073        13137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000073 DAZKNO010000000
VERSION     DAZKNO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.074"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..150)
                     /locus_tag="ACG8MY_001555"
     tRNA            complement(78..150)
                     /locus_tag="ACG8MY_001555"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Lys,seq:ctt)
     gene            531..1100
                     /locus_tag="ACG8MY_001556"
     CDS             531..1100
                     /locus_tag="ACG8MY_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0430748.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1087..1947
                     /locus_tag="ACG8MY_001557"
     CDS             1087..1947
                     /locus_tag="ACG8MY_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0430749.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2137..2841
                     /gene="yycF"
                     /locus_tag="ACG8MY_001558"
     CDS             2137..2841
                     /gene="yycF"
                     /locus_tag="ACG8MY_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0430750.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2846..4681
                     /gene="walK"
                     /locus_tag="ACG8MY_001559"
     CDS             2846..4681
                     /gene="walK"
                     /locus_tag="ACG8MY_001559"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0430751.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4678..5994
                     /gene="yycH"
                     /locus_tag="ACG8MY_001560"
     CDS             4678..5994
                     /gene="yycH"
                     /locus_tag="ACG8MY_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0430752.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5995..6864
                     /locus_tag="ACG8MY_001561"
     CDS             5995..6864
                     /locus_tag="ACG8MY_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0430753.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6919..7728
                     /locus_tag="ACG8MY_001562"
     CDS             6919..7728
                     /locus_tag="ACG8MY_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0430754.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7831..8460)
                     /locus_tag="ACG8MY_001563"
     CDS             complement(7831..8460)
                     /locus_tag="ACG8MY_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0430755.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8630..9922
                     /locus_tag="ACG8MY_001564"
     CDS             8630..9922
                     /locus_tag="ACG8MY_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0430756.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9998..10660)
                     /locus_tag="ACG8MY_001565"
     CDS             complement(9998..10660)
                     /locus_tag="ACG8MY_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0430757.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10885..11169
                     /gene="groES"
                     /locus_tag="ACG8MY_001566"
     CDS             10885..11169
                     /gene="groES"
                     /locus_tag="ACG8MY_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0430758.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11220..12845
                     /gene="groL"
                     /locus_tag="ACG8MY_001567"
     CDS             11220..12845
                     /gene="groL"
                     /locus_tag="ACG8MY_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0430759.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4367 a         2262 c         2833 g         3675 t
ORIGIN      
        1 ataaaaaaag aacagcgtaa atgttttcga aatgctttcg caccaacatt tacctgttcc
       61 gtgggtttgg cacccaatgg gccgtgaggg gctcgaaccc gcgacccgct gattaagagt
      121 cagctgctct accaactgag ctaacggccc gtacaaaaag tacttaataa tagtagcact
      181 aagaatttta aaatgcaaga ctacatttaa agaaaatcag aaaaatcttt acttccttat
      241 aggaaatcat tcatagatta ttcacatttg ctcgctatta tataagcata ccaacaaaca
      301 aagttcttgc aagagaacaa accctaacac ttttttcatt ttttactcct ttcccgccgt
      361 ctccccggcg ggtctcctgt tcttaacgat aaaagacaag aaggcttagc cttcttgttt
      421 tttgttttca tggaatcccg ctaatcaaat cacctagtta aagcaactag attgaatgtt
      481 tatcataagt ctgatatcat gtttatgaaa tgaaaaaagg agcggttatg atggaaaaaa
      541 tgcgtcagca attaatggag tgggggaagg aactagaaac attccgtctg cctcactggg
      601 aagaactccc tgacttagaa ctttatatgg accaagtcag aacgttagtt gatcgttatc
      661 tttcacctgt gatacaagga gaaaagcatc cattgcttac ctcttctatg gtaaacaatt
      721 acgttaaact aggtttgata ccagcaccag ttaaaaaaag atataacaaa gaacacgtag
      781 catttttact tgcaatcact acgttgaaac aagtattgac tattcctgaa atcaaagaag
      841 ggatattgtt ccaaggaaaa acagtaggaa ttcgtgaagc ctataatcta ttttgcgatg
      901 aacaagaagc agctgtgtgg atggtcagcc aattagcaca aggtaaatct catccgcaaa
      961 aattcgacca ggcaacccct gtagaatata ttgctgtaag agcggcaact ttatcgtttg
     1021 caatgaaact tcttgcggaa aaaacgattg ttctagaaac cgaatattta aaagaggaga
     1081 aaacaaatga aaaacaataa aatcgcaatt ttagtagatt caggtacgga tgtgccagaa
     1141 gaattgataa aaaaatacga gatgtatatg atcccattga aaattattta taaagatcag
     1201 gtatatacag ataaaattga cattacctct gaagagatct atcaacgttt gccagaggaa
     1261 atccctggta catctttgcc ggatggtgag tcgatcacag aaatctttga gcaaatcaaa
     1321 aaagacggtt atgaaaaggt acttgcggta acaatttcca gtggattgag cgggacatat
     1381 aatgtggttc gtttaattgc acaacagcaa gaagaattgg aagcatatgt actagatacg
     1441 aaaaatattg gtattggtgc aggtttttct gcgatccaag cagcaaaatg gctggaagaa
     1501 ggagtagaat gggatcaact gatcagtaat ctgaatgagt tagtcgaaag aacaaaagtt
     1561 ttctttaatg ttgcgacatt ggaatatctt caaaaaggtg gacggatcgg tttagttgct
     1621 tctattgtag gaaccgcttt aaagcttaac ccaatcatct catgtaatga agaagggatt
     1681 tattacacag taggaaaagc taggggacgt aaaaaaagtt tggataaaac ggttgcttac
     1741 gtcaaagaaa aaatcggtaa tgcaaaggtc ttcaacttag ctgttgccca tggggatgcg
     1801 aaagaagaag caattgaaat ggaagcgaga cttaaaagag agttcccaca agcaaaccaa
     1861 atctactttg gccagatttc tccggcatta gtagtccaca ctggtccggg attattgggt
     1921 gtcggtgtac aagttttaga aggataaatc aaaaaaagga aacactgagg tgaaaatcag
     1981 tgttttcctt ttttattgaa gttagaacaa aaaataataa agtattttgc actttcattt
     2041 taattttctc ttttgtttta taggcaaaga tgaaacaaaa gcagaaatac ggtataatga
     2101 gaaggtatga caataatata taagaggtgg ctctttatga aaaaagtttt agttgtcgat
     2161 gatgagaagc cgatttcgga tatcgtcaaa ttcaatttag ccaaagaagg ctatgatgtt
     2221 tatactgcat atgatgggga agaagccttg gaaaaagttg ctgaggtaga acctgatttg
     2281 attttacttg atttgatgct acctaaaatg gacggcttag aagttgctcg tgaggtacgc
     2341 aaaacttatg atatgccgat cattatggta acagccaaag attctgaaat tgataaagtc
     2401 ctaggcttag agttaggcgc cgatgattac gtgaccaagc cattttccaa ccgagaatta
     2461 gttgcacgtg tcaaagcaaa tcttcgtcgc ggtgccacgg ctgcgaagga accagaagag
     2521 gcagcacctg cggaattgac gattggtgat ttgacgatcc atccagaagc ttacatggtg
     2581 acgaaacgag gagaaacaat cgagttaaca catcgtgaat tcgaactact cttttacttg
     2641 gcgaagcacc ttggacaagt gatgacaaga gaacatttat tacaaacggt ttggggttat
     2701 gattattttg gagatgtacg aacagtggat gtaactgtac gtcgtttgcg tgaaaagatc
     2761 gaagataatc caagccatcc aaattattta gtgacacgtc gtggtgtcgg ttactatttg
     2821 cgtaacccag aacaggagta attggatgaa aagaaaagtc cgcttttttc gatcagtcaa
     2881 ttttaagatc gctattacct ttattctgat tttattgatc tcgatcgaaa ttattggtgc
     2941 ctactttata cgtggtttag aacgttccac gatcaataca tttattaaag atatgaatca
     3001 aacggtggaa tcattagcaa caacaatcag cccggaattg aatcgaaaag ataatgcaga
     3061 tgatgaagag gtaaatgcga atatcaaaag atttattgaa aacagtgcga ctagcgatat
     3121 catcgaaata cgggtcgttg acgaaaaagg gatcatccga ggaacaaccg atgtcaatga
     3181 acaaagcgct gtgggaaaga aaaatgatta tgttgatatc aatgatttca cgactaaacg
     3241 ttatgttgct ttagataacg acgataaacg ggtgaatatc aatgttcaac cgatcctgtc
     3301 cccgaccggc gatgccgtta tcggggctct ttacgtcaaa agtaatatcg aacaaaaata
     3361 ttcggaaatc aataacacag cagtcatttt ctttactgcc tcactaattg cggcatttat
     3421 ttcaatgatc gtatctgttt tagttgctcg ttcgattaca cagccgatcg gtgaaatgag
     3481 agaacaagcc attcgaatcg ctagaggaga ctacagtcgg aaagtcaaag tgcatggaca
     3541 agatgaactt ggacagctgg ctgatacctt caatcagtta gctgaacgta ttgaagaagc
     3601 tcaagataca atggaagctg aacgcaatcg tttggatagc gtgctatccc atatgacaga
     3661 cggcgtcatt gcgacagatc gcaggggtaa agtcattacg atcaatgata tggctgtttc
     3721 cttgctagat atcaaaaacg aggaagcaat cggacaatcg attttgacac ttttagatat
     3781 cgaagaagaa tatacgttgc ggaaattatt agaagatcca aacgaaatgg tacttgaccg
     3841 ttcaaccagt acattggaaa gtgatcagat gatcttgcga gtagactttt ctatgatccg
     3901 tcgcgaatca ggttttatca gcggattagt agctgtcttg catgatgtga cagaacaaga
     3961 aaaaacagaa cgggaacgtc gtgaattcgt ctctaatgtt tcccatgaac ttcgtacacc
     4021 gttgacaagt atgcgtagtt atatcgaagc attgagcgaa ggtgcgtgga aagacgagga
     4081 agtagcacca aattttttga aggtaacgct agatgaaaca gaccgaatga ttcgaatgat
     4141 caatgacctc ttaaatctat ctcgcatgga tacaggtaat acccagcttc aattagaata
     4201 tgtcaatttc aacgagttag tgaattttgt tcttgaccgg tttgatatga tggtgggaaa
     4261 tcaggaaaaa aattataaga tccgtcgtga atttacgcaa agagatcttt gggtcgaagt
     4321 agatacagat aaaatcatcc aagtagtcga taatatcatg aataacgcga tcaagtattc
     4381 accagatggg ggaacgatta cttgtcgctt attagaaacg cataataacg tggttttaag
     4441 tatcaccgat caaggattgg ggattcctaa aaaagatctg aaccgagtgt tcgagcggtt
     4501 ctatcgagta gataaagcac gtgcaagagc acaaggtggg acaggacttg gattagctat
     4561 ttctagagaa gtgattaagg cgcatcgagg cgctatttgg gcagagagca aggaaggaaa
     4621 agggtcgact ttttatatct cgctgccata tgaaccttat gaggaggaat ggtgggaatg
     4681 agaatctctg aaaaagtcgt tcgtatagga ctcattttga tggttgcgtt aagcatatac
     4741 ttctcttatg caatctggct gagcccagca ggaaaaagtt caatcaacgt agatgagaca
     4801 aacactcaaa tgatcgagtc tcaaagcaat cgaaaaactt ccgaagtctt tcttccgctt
     4861 catgttacat ggcaccattc gggaatgatc caagaaacca acagtgaaaa tctcgtgtac
     4921 cgattgcaat tgatcatcga taaagcaaga tttggaaagc tttctgaagt agtttcagga
     4981 gacaaatcac agtttgaaca ggtgaaaaat ctgaactctg gttttgagtt ggcctataat
     5041 gcgccttttt tgctaagaga atacaaggaa gcatttgatt tagatttaga tgtgtcgaac
     5101 ttggcaaaag aaagcgaaaa tatgtatttt actagaatac aattcgatca aggaaataaa
     5161 aaaatccgat ttttgaatta ttcgaaccat ctcgtatatg aagcaaatat ttcgttgaat
     5221 tggacagata tcaataaaga attaacagca aaagacacaa aatggacaga aatgacaaca
     5281 gaaccatcga cgatcgacac gcaatatgct acgaaacatt cgatcaaact gaaaaagtat
     5341 agttatatcc tgtcacagca accctataca ctttttagaa atgctttttt ccagaaacca
     5401 gatagcgtga aaaataatga tgaaagcaat gaattgattt tttacggtgg gaatgaaaca
     5461 atgtctatcc attcggaaac gcaagtagtt gattttcgtg gagagctgcc agagaaaaaa
     5521 gaaaatgcta gtgtattttc tgaaagtttc ccctatatta gcaaattagg cagttcttta
     5581 gggaacctca gatattttga ccgcagtgac aatacgatcg attatcggat attcgtggaa
     5641 ggcttcccgg tttttgggac agacagcaga ggacagattg gtgtggaagt tggcggtgaa
     5701 acgaatggtt ctgtgcaagt gaatatccaa accagtttga atacgattca agtgccgata
     5761 ccttcagacg aagaagtaga acttgcaagt acgaatgaaa tcatccagca gttaaaagct
     5821 aagggagcag atagcaacaa gattggttca gtgatcgttg gctatacatg gcaaaatgtc
     5881 aaagaaacga atcgtgtcgt tgatctctta cctgaatggt atgtgaaata taaagataac
     5941 tggtattcag ctaacgacct tctggaaaaa ttacctggat tggaggcgaa ttaattggat
     6001 ttcaaaaaaa tcgaatggat atttttcgtc gcttttttag ggttgaatgt gtttttattc
     6061 agcagttatc atgacgcggt ataccaagaa aacaatgtca ttcgctctaa tcagactgaa
     6121 tcaatccaac aacgtctggc tagcgacgat atcacttaca cggggaagat ttcgtctgag
     6181 aacaagcaag gctattatct tagcgcggag cagaccaatc ttagcacttc attagcgaat
     6241 tatcgcaagg ctggtaaaag cggcttgctg gcaagtggtg tgtttttaga cggtgatgtt
     6301 ttgacgaaat ctttatccag ttcagatgct gaaaagaacg tgatcgatcc agataaaatt
     6361 agtagttcct tggaagattt tttatctaac aaagacaata ttttattcgg taatgattat
     6421 gtgtacacga aaaacttttc tcatttagaa gaagaaccac tagaaatcca agcaagccaa
     6481 gagtatgaag gcatcccatt caatgatgat acagcaaaaa tcgttctttc tttagaaaaa
     6541 aacgataatg attatagcat cacgaaatac atgcagacgc atttatctga tatcgagcag
     6601 ctgcgcgaaa agacggaact gtatacggaa gaggaagcaa tcaatacatt gtacatcaat
     6661 aacaaaatac caagaggatc aaaaatttta tggcgtcagc tagcttattc ctgtattctg
     6721 aaagtccggg aaaaaaatgt gtatgttcct gtttggcatg tagcgatcga aacaagcgat
     6781 aaagtggtcc aaattgagag tgtcaacgct ttcagcaata cgatcgtgac aaacaatact
     6841 gtaccaaaag tggaagatca ataaaaaagc gagtataatg aaaaaagttt acaaagtaag
     6901 aagggaagta ggagtcaaat ggctgaagaa aaaccagctt ttaaaatcag tatcctagca
     6961 agtggaagtt cgggtaattc actttatatc gaaagtgaaa aaaaacggct tttggtcgat
     7021 gcaggactta gcggtaaaaa aatcacttcc ttgatggctc aaattgatcg gacaccggac
     7081 caattagatg gaatacttgt gacgcatgaa catcgagatc atatacatgg cgtgggtgtc
     7141 ttggctcgta agtatcattt ggatgttttt gccaatcagc agacttggga agcgatggaa
     7201 ccactgattg gaaatgtacc agtggaacaa aaacatctat ttgagatggg gaaagtcaga
     7261 acattcggcg atatcgatat tgagagtttc ggcgtctccc atgacgcagc ggctccgcaa
     7321 ttttatcgtt tccataaaga aaatcggtca ttcgtcatgt tgacggacac cggatattgc
     7381 agtgattata tgcgaggtgt cattgaaaat gccgatggtt atttgatgga aagtaatcat
     7441 gatcttgaga tgttacgcat gggaccttat ccgtggaatt taaaacagcg tattttagga
     7501 gatagaggac atctttccaa cgaagacggt gcacttgtca tgactgatgt gatcggtaat
     7561 cggaccaaac gaatctatct tggccatctt agcaaggaaa acaatatgaa agaacttgct
     7621 catttaacaa tggaaaatgt tttgaaagaa aaagattttg gcgttggaca tgattttgaa
     7681 atctatgata cagatccaga tgaagcaact gagctatttt cgatttaaac agttcaatca
     7741 ataaaaatat aaaaagcaga agataaaatc aatcagggtt ggttttatct tctattttta
     7801 tttttttagc atattaaaaa aataaccgta ttatcgactg actacactga tgtgctgctt
     7861 ttcatgtttc tcatccagta tctcatctac taatgcaatc gcgtaatctg ccatgctgat
     7921 cgtactgtcg ccagcagcgt ttctttgtaa ataatcatca cttaacaaat aagtacctgt
     7981 acgtttgcca tgtggatcaa ataaagaagc tggactgata taagtccaat gtagattttt
     8041 agattgtttc aattttaaca atccttcgta catattgaaa gctgttggat agaagggtgc
     8101 atttttaggt acttggtcaa tcatacgagt cgtcttttct gtatctaaga ataacgaaga
     8161 ggcaccccca acgacaatta agcgtgtatc ggttccttca agcatctcaa tcaaatggga
     8221 caaagaagtt tgatgcattt cttcttttcc tttgggagcg ttgaaagcat caacgacaac
     8281 atcaaaagat gcaagatcag tagttgtcaa ctcaaataaa tccttttgta taaacggaac
     8341 atctgatggc aatttttctg gatgacgcac gattgccgtc acgtctaaat ctctggacaa
     8401 tgcttcgtct aaaataaatg aacctgcatg gccagtagct ccaatgagtg caattttcat
     8461 aaaaaacctc cttttttatt cctgtccatt gtaagcagtt gctgaatatt cgtcaaagaa
     8521 tccgattttt gaaaagctca gtgcgcattc ctttcatggt tcgtgatcat tgggtatagt
     8581 taaattaaga aaactgattg ataaaaatag aagttgaagg agataagaaa tgaaagagtg
     8641 gttagatcgt gcctttttac caatgataac atctgttgtg aatcttagac ccttaactgc
     8701 tttgcgtaat ggaatgactt acacgatccc aataattatt gttggctcgc tgtttttctt
     8761 atttgctcat tttcctgtgg attacatcgc tcagtggata gacaaaacag gattttctat
     8821 gtattttaca caagtatacc aggctacatt tgatgtgata gccttttgga tcgtttttgc
     8881 cattgcatac gcttatgcaa aagaagaaga catcgatcgc ttaccagtcg gcataacagc
     8941 cttgtctgct tatctactgc ttattcagcc actaaatact cagatagata ctgtctggac
     9001 aggtgctgaa gggatactag gagcagttac aactgggctc atcatcggat ggggctatgc
     9061 acgtttagta agacttgcga aagggtggaa gcaaagagag aagatacctg aaaatgtttg
     9121 gcagtctttt acttcattga ttccaatcat tgttatagtc acttttacgc ttatcggttc
     9181 agcggttttt caacagttgt tcagaatgac tgttgttcaa ggagtatgga catatgtcca
     9241 gcttcccatt caatcgttca tagacacgtt gtttggtgtg cttttattag gtttttttgt
     9301 tccgttttta tggttatttg gtcttcatgg atctgcgatg gtcaatgggt tggttagtcc
     9361 cgtcttgcaa gcgaattcat tagcgaatgc agaaatctta gcctcaggac aagagttgac
     9421 ggtggcaaat ggcggacata tcgttactca gcaatttctc gatcagttca tgacggttac
     9481 gggtgctgga ttgacgttag gggctgtatt ttttatgacg gtttttgcga aatcgaagaa
     9541 atacagagaa ttaggaaatc ttgctctttt accagccttt ttcaacatca atgaatcaat
     9601 tattttttcc acaccgatcg tgatgaaccc gatgatggct gttcctttta ttttcgcacc
     9661 ggtcgtttca ggattgatta cctattccgc tttatatttt ggattcgttc ctttatttac
     9721 agcagtccaa gtcccttgga caacaccgcc gatcctctcg ggtttcttag taggcggagt
     9781 gcctgcagcg attttacaag gtatcgtgtt gtcaattagt tttttcattt atttcccatt
     9841 tttgaaaaaa atagattctg caaactgcaa aaaggaaaga cagacaaaaa atgatggagc
     9901 agcagaaaag atgaacgact aaaaaacaaa aatactcctg tcgctttaga ttgggaaaat
     9961 caccaatcta ggatgacagg agtatttata gtcatgttta tactagccct ttttggacag
    10021 ctaattgaac aagaacaact aatgtgttca ttcctacgtg tgtgatcatc gatgtccaga
    10081 tccgaccagt tgctttgtac tctaggctga agaagaaacc taggaaaaag tagattagca
    10141 gatgaccatc attatgagca aacgcaaaca gcaaagagct gatcactgct ccgacccaaa
    10201 aatttgaata tcgactgatg atccccagta tactgcggcg gaatacgaac tcttccatga
    10261 tgggtccagc aacagttgtc gccaaaataa aagcgggtgc ttccatgatc atctggatga
    10321 tattttgtgt attctcggaa ggggttgctt tgccaaacaa tacagattct aacatgacag
    10381 cgatgctttg cagaatcaat gcgacaaaga ttccaaccaa tccataaata acaatctttg
    10441 gagcagaagc tttttttacc gctgattcga tcgctaaggg ttcacgttgc ttaaaataaa
    10501 aaatagctag gacaacggcg cctagtatat acatgactgc agttgctgtg atcaattcag
    10561 acgtagattt tacaagtcct atcgaagcta ggatcaaggg tgagataaaa attagaccat
    10621 agcaaaaaat gctgaggaag ctatattttt ttaaagacat gtaattcctc cttttaaggg
    10681 ataacaagca tgcaaggcaa gcttatatcg attgatttca attatatcat aatttcaata
    10741 agcagctgtc agaataaaat ttcttttctt tgatttaaaa gcttgcaaaa aagagggaga
    10801 atgagtatta ttaaaaatgt aggttagcac tcgaaggtta agagtgctaa cagattaaaa
    10861 aaagagttat cggagggatt tatcgtgtta aaaccattag gtgatcgcgt cattatcgaa
    10921 gtcgcaaaag aagaagaaaa aactgtcgga ggaatcgttt tagcttctgc tgctaaagag
    10981 aaaccccaaa ctgggacagt tgttgcagtc ggtgaaggcc gtttgttaga aaacggtgaa
    11041 aaagttcctg ccgctgtaaa agcaggcgac caagttatgt tcgaaaaata cgccggtaca
    11101 gaagtaaaat acgaaggaaa agaatatctg atcgtcgcag gaaaagatat catggcgatt
    11161 gttgagtaaa tttggaagat aaaaaaataa tcaaaaataa tcaatgaggt gaatcaatca
    11221 tggcaaaaga attgaaattt gcagaagacg cacgcgcagc tatgttacgc ggggtagata
    11281 aattagcaga tacagtgaaa gtaacattag gacctaaagg ccgaaatgtc gtactagaaa
    11341 aatcttacgg ctctccattg atcacaaatg atggtgtgac gatcgcaaaa gaaatcgaat
    11401 tagaagatca ttttgaaaac atgggtgcaa aacttgtatc agaagtagca tcaaaaacaa
    11461 atgatattgc aggagacggt acaacgacag caactgttct gacacaagca atcgttcgcg
    11521 aaggtttaaa aaacgtaact gcaggtgcta atcctctagg cattcgtcgc gggatcgaat
    11581 tggcaacaaa agcagcagtc gaagaattgc ataatatctc aactgttgtt gattcaaaag
    11641 aagccattgc acaagtagca gctgtttctt cgggttctga taaagttgga catttgattg
    11701 ctgatgcaat ggaaaaagtt ggaaacgacg gtgtcatcac aatcgaagaa tcaaaaggga
    11761 tcgaaacaga actagatgtt gtagaaggta tgcagtttga tcgtggttac ttatcacaat
    11821 atatggtaac agataacgac aaaatggaag ctgttctaga aaacccatat atcttgatca
    11881 cagacaaaaa aatctctaac atccaagata ttctgccatt gttagaacaa atcttgcaac
    11941 aatcacgtcc attgttgatt attgctgacg atgtagatgg tgaagcattg ccaactcttg
    12001 tattgaacaa aatccgtgga acattcaacg tagtagctgt aaaagctcct ggtttcggcg
    12061 atcgtcgtaa agcaatgctt gaagatatcg ctattttgac aggtggtaca gtaattactg
    12121 acgatctagg tttagaattg aaagatgtaa caatcgaaaa cttaggaaat gcttctaaag
    12181 ttgttgtaga taaagacaat acaacgatcg tagaaggttc tggagaaaaa gaagcaatcg
    12241 aagctcgtgt gcaattgatc aaaaaccaaa ttgctgaaac aacttctgat tttgaccgcg
    12301 aaaaattaca agaacgctta gctaaattag caggtggtgt agctgttgtt aaagttggtg
    12361 cagctactga aacagaattg aaagaattaa aattacgcat tgaagatgca ttgaatgcta
    12421 cacgtgccgc agtagaagaa ggaatggtct caggtggtgg tacagctcta gtgaacgtta
    12481 tcagcaaagt ttctgctgta gaagcagaag gagacgtggc aacaggtatc aagatcgttg
    12541 ttcgtgcttt agaagaacca attcgtcaaa tcgctgaaaa cgctggttat gaaggatcag
    12601 tgattgttga taaattgaag aacgtggaac taggcactgg tttcaatgct gctactggag
    12661 aatgggtaaa catggttgaa gcaggtatcg tagacccaac taaagtaaca cgttctgcct
    12721 tgcaaaatgc cgcttccgtt tctgccttgt tattaacgac agaagcagtc gttgcagata
    12781 aaccagaacc agcagcgcca gcagctcctg caatggatcc atcaatgatg ggcggtatga
    12841 tgtaagaaaa agcaacttgc ttatctacaa ctgatcatta gtgaaagaca gataaaaaga
    12901 ggctggaaca gaagcgttta actccaagaa ataagaagaa attcacggaa attgcttttc
    12961 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ctgtttatac
    13021 gtgtttagag cgtgagacaa aagtgttctt tacttttgtc ccacgctctt ttttgtgtgt
    13081 aattataatg aaaatagtta tttttaatag ttataatatt ttatctatta aaagtaa
//
LOCUS       DAZKNO010000074        13085 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000074 DAZKNO010000000
VERSION     DAZKNO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.075"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACG8MY_001568"
     CDS             complement(78..2198)
                     /locus_tag="ACG8MY_001568"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0430760.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACG8MY_001569"
     CDS             2428..3606
                     /locus_tag="ACG8MY_001569"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0430761.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8MY_001570"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACG8MY_001570"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0430762.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8MY_001571"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACG8MY_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0430763.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACG8MY_001572"
     CDS             complement(4959..6212)
                     /locus_tag="ACG8MY_001572"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0430764.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8MY_001573"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACG8MY_001573"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0430765.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACG8MY_001574"
     CDS             7789..9135
                     /locus_tag="ACG8MY_001574"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0430766.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACG8MY_001575"
     CDS             complement(9443..9829)
                     /locus_tag="ACG8MY_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0430767.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="ACG8MY_001576"
     CDS             complement(10097..10246)
                     /locus_tag="ACG8MY_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430768.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10708..10920
                     /locus_tag="ACG8MY_001577"
     CDS             10708..10920
                     /locus_tag="ACG8MY_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430769.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="ACG8MY_001578"
     CDS             complement(11198..12997)
                     /locus_tag="ACG8MY_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0430770.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaac
//
LOCUS       DAZKNO010000075        12987 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000075 DAZKNO010000000
VERSION     DAZKNO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.076"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..1175
                     /locus_tag="ACG8MY_001579"
                     /pseudo
     CDS             34..1175
                     /locus_tag="ACG8MY_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1301..2380)
                     /locus_tag="ACG8MY_001580"
     CDS             complement(1301..2380)
                     /locus_tag="ACG8MY_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0430771.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2483..2923)
                     /locus_tag="ACG8MY_001581"
     CDS             complement(2483..2923)
                     /locus_tag="ACG8MY_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0430772.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(2980..3390)
                     /locus_tag="ACG8MY_001582"
     CDS             complement(2980..3390)
                     /locus_tag="ACG8MY_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0430773.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3570..4004)
                     /locus_tag="ACG8MY_001583"
     CDS             complement(3570..4004)
                     /locus_tag="ACG8MY_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0430774.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4104..4952)
                     /locus_tag="ACG8MY_001584"
     CDS             complement(4104..4952)
                     /locus_tag="ACG8MY_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0430775.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5341..7107
                     /locus_tag="ACG8MY_001585"
     CDS             5341..7107
                     /locus_tag="ACG8MY_001585"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0430776.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7209..8468
                     /locus_tag="ACG8MY_001586"
     CDS             7209..8468
                     /locus_tag="ACG8MY_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0430777.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8615..9916
                     /locus_tag="ACG8MY_001587"
     CDS             8615..9916
                     /locus_tag="ACG8MY_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0430778.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9919..10770
                     /locus_tag="ACG8MY_001588"
     CDS             9919..10770
                     /locus_tag="ACG8MY_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0430779.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10783..11598
                     /locus_tag="ACG8MY_001589"
     CDS             10783..11598
                     /locus_tag="ACG8MY_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0430780.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11749..12915
                     /locus_tag="ACG8MY_001590"
     CDS             11749..12915
                     /locus_tag="ACG8MY_001590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0430781.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         4432 a         2339 c         2431 g         3785 t
ORIGIN      
        1 ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat cttaatatca
       61 acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac agaggagaca
      121 gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac ctttcaggat
      181 gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac agcgaccatc
      241 aaagcaaaaa ttgactattc gggagactta gcacctatac tacaatcagc tgttacattt
      301 gagaatgttg atcccacttt gtcgattggc tatgacgaga acactttgac tatccaacac
      361 aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa accagcgctt
      421 tttactgtca aggtatcaga tggactaccg aacgatcatc atttaacgcc gtatagtcag
      481 cggcagacaa ttgagcaaga ttcagccatc gactcaggag gagatcttgt tgaagaacca
      541 acagataaac cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga
      601 gaacagaaac cggaaataga acctggagaa gaacccgata cagaaactca accagagcca
      661 gacaataaat caaaacctga aataacccct ggagaaaaac cagatgtaac tccagaacca
      721 gatacggatt ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact
      781 gaaaatccag aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac tccagaacca
      841 gatacggacg caagagacca aggaatacca gaaaaaataa acaaaaaaac aattcaagag
      901 gatggaaaaa aagagagtaa aaaatcaaat ctagcaattc taaaaataaa tgaagagcag
      961 ctgaataaaa aatcacgaat atttgatagc gcccaatcag ctgaaacctt aaaaagtagc
     1021 aaggatacaa cctttgctgt cctgaaacga aaaataaaca gctaccaaaa tccggagagt
     1081 cacaaaacaa agttatactg tggagcggaa tcattctatt aagtatagca acaatgcttt
     1141 ctgcaaaaag atttaaacaa aacagatctc tctaacattt gaataatgtc tgtgaacgca
     1201 tttttcaaat aaaaagaaat atccagaaaa tgaccccaaa ataccagatt gaaatctgat
     1261 atttggggtc gttcatactt tatctttctt catatttatt ttaagggata tctgtttctt
     1321 ttggaagatg gttcgggatc tttttcactt tcaatacatc taaaaagaag gtaaagatcg
     1381 gaatgccgac aatcaagccc caaacgccaa agaaccgttc gctaacaagt aagaccacaa
     1441 atgtataaaa aatcggcagt tctgttttgc tggacatcaa cttcggattc agcacataag
     1501 actcaattag atggacaatc aaaatcgtaa tcaggatata aacaacatct cggatgcctc
     1561 cttgggaata agctatgaaa gatagaggaa tacaagaaat gataacacct gctactggga
     1621 tcagacttaa aatgaaaatc atgatcgcta aactcagcag ctgatgaaag ccaaatacag
     1681 ccaaggcgat tgtagtgagc aacgtattta aaagagcgac aacaaattga gcttctaata
     1741 ccaaaccaaa tgtattgaca aacttatcgg caaaatagta aatatcttga aagaaccaag
     1801 aaaattcact ttttaaaaac aatcttgaga atacaattgt ttttttcttt tcaatcataa
     1861 aaaagaaact taagatgaat gacatagcaa acgccactgc catttttcct atatcctgta
     1921 aatagccaag cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa
     1981 tataagtatg gatatattgt aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat
     2041 taataacgga ctggatcatg tcaaatgttt gatgaataag catcgggata tactttgtga
     2101 atgccagata gataaggtaa acgatcagtg tataaatcat gatagctaaa acacctgtag
     2161 ggattcggac aaatcgctga atgatttgaa caagatgaat catcaaataa gtaaagataa
     2221 atgtgagcaa aatggttgta atcatacttc taactaagaa aagaacaaaa acgacaaata
     2281 ataaaacagt gagacggcgt attttttcat tcgccaaaaa acgctgataa acggtcaaat
     2341 cgacaacctc caaataacaa aaaataaaca cgttattcat tatatgatgg aatatgaaaa
     2401 taagaaagag caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca
     2461 taaaatcgtt catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca
     2521 tttttcgggt cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact
     2581 tgatcttttg tggaatacct tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg
     2641 taacatttac gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg
     2701 ctaacttttt tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct
     2761 ctttcactga agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg
     2821 taagattcag cggcttgagg aggggagtgt agtgtctaat gatttcttta gaggcggcat
     2881 ataaaggaaa acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt
     2941 tttactatag tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag
     3001 agtaacttct atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc
     3061 taataattct tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc
     3121 taagacaaag ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc
     3181 cccagagata tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa
     3241 accagctgca aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc
     3301 aatattcaat tcgaatgtac gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt
     3361 gatcattttc gtagaataca tttttttcat aaagttacca cctttaatta tattttatac
     3421 aattgaattt tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat
     3481 aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc
     3541 agagacaaaa aataatctca tcataaacat tacatatatt tctcaaaaag ttgttttctt
     3601 tcttcaaaaa ttacttcacg atggacctga tgaagcatcc ataaataacc aaaaggatct
     3661 ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg aatctctaca
     3721 cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat gatattgaac
     3781 cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt gttttcatcc
     3841 agcatatgga atctcgtgcc atacaaggta aaaatgactt cattattgcc tctagggtaa
     3901 tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc tagtgctttc
     3961 aaactatcat ttaatacaaa atctatttcc gttcctttat gcataaagat ctcctctcct
     4021 tttcagtcga acagttacta gattctttta ttataccagt taaagaaaac gcattcaaaa
     4081 aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc gcgtcccatt
     4141 acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc tatcaatggt
     4201 ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt tgactttcga
     4261 ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc acttaagcga
     4321 aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt tcgattgata
     4381 cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat agcataaaga
     4441 aactcttcta ttccaaaaac aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat
     4501 aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta
     4561 ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa aatcgcactt
     4621 ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac tattgcaatg
     4681 attaaaggaa aaaacaagct tccgaaaagt actttaaaaa agggtttttt acctaaaaat
     4741 agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc tcggtcaaat
     4801 cctaaaacag cttccagtaa tattcctaga ccgctgacac ctccagcagc aatatgatga
     4861 ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat aatcttaatg
     4921 tatgtccata taaattgtct cgtttctttc atttatccac ttccttaatt cattaataat
     4981 actcttatct tattataact tacttttcac cttaagttaa ctttaattaa taaaaaaatt
     5041 aggctagtct aaattattat gtttcattct tctagaatat acttctacgt taaaaactgt
     5101 taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa ttcaggacac
     5161 taaatagtac aggaagtaga ttaaaacttt taaaagtttt aagtctatct atttggcaag
     5221 tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt gaaagcgttg
     5281 tctttcgttt ctctatcact tatagtgatg acgtcatatt aaaacacaag gaggaaatca
     5341 atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct atatacgaat
     5401 acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt atatgtgctt
     5461 tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg accaatgaaa
     5521 aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag cgaaacaagg
     5581 cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt ttacggtgat
     5641 caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta ttttcgaata
     5701 ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga aacagtatgg
     5761 tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc cgaacacact
     5821 ttaccttggg gaagtaaaga tccagaccga gaagacttct tcgggggaga tttgcaaggt
     5881 gtgagggatc atttggatta cttgacggac cttggcgtca atggcatcta tttttgtccc
     5941 atatttaaag ccacatccaa tcataaatac gatacgattg attattatga ggtggatccc
     6001 gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa aagagggata
     6061 cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca gtggcaagac
     6121 gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg ttttttccca
     6181 gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc atatgatact
     6241 tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt gcaaaaatat
     6301 ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg gcgtcttgat
     6361 gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt tacagaaatc
     6421 aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg gctgcaaggg
     6481 gatgaattcc atgcagtgat gaattatgca ttcacagatt cgattaaaga ttattttgca
     6541 aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca aatgctttac
     6601 agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac tgccaggatc
     6661 ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt tatgtttctt
     6721 caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg tgaagatgac
     6781 ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga gctattcggt
     6841 tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc tgaaggttct
     6901 actcagtggc taatagtata tgatcgtgaa gataagctct atttcacacg ggaactagag
     6961 ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt agaacaggaa
     7021 aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc agagatacga
     7081 caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg
     7141 caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat acacaggagg
     7201 aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc tctttaggaa
     7261 tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg gaagccgatg
     7321 aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata aacgaaataa
     7381 aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag gacatgttcg
     7441 atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac gttatgatgg
     7501 cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa gtaaaactgg
     7561 gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat ggaaaaatct
     7621 acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat ttagtcgata
     7681 cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt tttgcttttg
     7741 agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt tattattcct
     7801 atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca aatccttcag
     7861 atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca gattggttca
     7921 aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc gccagccagc
     7981 aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg caaacataca
     8041 aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat ggacagcctt
     8101 atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc
     8161 tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa
     8221 tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct aaaaacgatg
     8281 aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca aacatcccag
     8341 aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa
     8401 aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc atagaacaaa
     8461 aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt gcttcagata
     8521 aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc ttaaaagcag
     8581 tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa caagcaatat
     8641 caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt ttgtaaatgg
     8701 ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg gtcagatgct
     8761 tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac caatggaaga
     8821 tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa tcattttctt
     8881 gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg
     8941 caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg aaggttttcc
     9001 ttatctattg acacttcccg cttatcttct catgattttt acgatcattt tcccagtcct
     9061 agttacattg ttcactgcat ttactaatta tgacttcaat catattccac cagccagttt
     9121 aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct catatcgtaa
     9181 tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa caactttgca
     9241 aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta aattcaaacg
     9301 tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta ctatcatgag
     9361 tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga ttcctttcat
     9421 aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc cgaactggac
     9481 gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct atgtgatggt
     9541 tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc
     9601 aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag tggcagcgcc
     9661 aacatttatt acacagtata cggggaattt caataatttc tccatgatct atctgtttaa
     9721 caatggtggt cccggaagcg taggcggagg agctggttct actgatattc ttatttcctg
     9781 gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag caatcacgtt
     9841 gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt
     9901 caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca aaaacgactt
     9961 tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat ctatccgctt
    10021 ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag tttagatctt
    10081 tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact ttacgggact
    10141 tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt gactgtagta
    10201 actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc aagtttgatg
    10261 ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt ttatgtcatg
    10321 gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta tataggcggt
    10381 ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc aatagattta
    10441 gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca gattgtatta
    10501 ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg accttttggg
    10561 gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt tgctgtcggc
    10621 ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc tgctggggca
    10681 attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa ctttgttacc
    10741 ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac atttccgtta
    10801 aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa actattatca
    10861 tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat gcctgtgact
    10921 cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc cgttcattcg
    10981 ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga aactgggtta
    11041 caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt gtctttatct
    11101 gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca atgggtgatc
    11161 caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc atctttccaa
    11221 ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga attttatgcc
    11281 agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt cctatttgtc
    11341 atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag aaaaagccaa
    11401 ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt tatgggaata
    11461 ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt gatgctaagt
    11521 gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa
    11581 gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt gaaaaaaatg
    11641 aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt tgattctttt
    11701 cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat gttagagaaa
    11761 actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg
    11821 gacggtacaa cgaaacaata cggagatacg gacactcctc cccaaatcaa aatcaatatc
    11881 catgaagaaa tcccaatgaa agagattaca agtaacgctt ctttggcact aggagaagcc
    11941 tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa cgatgcttat
    12001 cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc aaagaaagaa
    12061 aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt aggtaacgac
    12121 ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt caatacacct
    12181 gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga caaactcttt
    12241 atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt gatgttcact
    12301 gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga acaatacgat
    12361 ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt attattaatg
    12421 gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg tatgttcgaa
    12481 catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt attgaaacca
    12541 aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg
    12601 atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt agttgataat
    12661 attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga ctaccaattg
    12721 acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac gaaagaaatg
    12781 ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc ttcatttgaa
    12841 tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa tgacatccca
    12901 atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag
    12961 ttttatgatg ctccagaagt gaacttt
//
LOCUS       DAZKNO010000076        12672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000076 DAZKNO010000000
VERSION     DAZKNO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.077"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(246..749)
                     /locus_tag="ACG8MY_001591"
     CDS             complement(246..749)
                     /locus_tag="ACG8MY_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0430782.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1108..2133
                     /locus_tag="ACG8MY_001592"
     CDS             1108..2133
                     /locus_tag="ACG8MY_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0430783.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2456..3334
                     /locus_tag="ACG8MY_001593"
     CDS             2456..3334
                     /locus_tag="ACG8MY_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0430784.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            complement(3544..4911)
                     /gene="nhaC"
                     /locus_tag="ACG8MY_001594"
     CDS             complement(3544..4911)
                     /gene="nhaC"
                     /locus_tag="ACG8MY_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0430785.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(5020..6441)
                     /gene="tyrP"
                     /locus_tag="ACG8MY_001595"
     CDS             complement(5020..6441)
                     /gene="tyrP"
                     /locus_tag="ACG8MY_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0430786.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(6684..8561)
                     /gene="tdc"
                     /locus_tag="ACG8MY_001596"
     CDS             complement(6684..8561)
                     /gene="tdc"
                     /locus_tag="ACG8MY_001596"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0430787.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(8852..10108)
                     /gene="tyrS"
                     /locus_tag="ACG8MY_001597"
     CDS             complement(8852..10108)
                     /gene="tyrS"
                     /locus_tag="ACG8MY_001597"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0430788.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(10220..10425)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10688..11989
                     /locus_tag="ACG8MY_001598"
     CDS             10688..11989
                     /locus_tag="ACG8MY_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0430789.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(12058..12657)
                     /locus_tag="ACG8MY_001599"
     CDS             complement(12058..12657)
                     /locus_tag="ACG8MY_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0430790.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         3888 a         2468 c         2299 g         4017 t
ORIGIN      
        1 tcctttctag attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa
       61 aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg
      121 tcctgctgca ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg
      181 aataaaaaga ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa
      241 cgattttatt gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag
      301 aataaaatgt atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg
      361 tttagaagtt gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca
      421 gtgatgtccg ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca
      481 aggaaaataa tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc
      541 accccaaaag gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt
      601 tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat
      661 tgtggaagat taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc
      721 cctaataaaa attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat
      781 cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta
      841 cgaaaattag aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg
      901 agttgcttgg aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt
      961 gttctaattt ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata
     1021 gttgttatac taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt
     1081 ttccaattac taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata
     1141 caagataatc cttttcgtat catgaatctg atgctactta tctttattgt attatggtta
     1201 ggttacataa atcatgattt tttgatttca agtttttcat cattagggat ctttactttt
     1261 ttctattatc aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc
     1321 ggactgttgg ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca
     1381 tttgccgtag ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct
     1441 aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca
     1501 atcgctcaat taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt
     1561 gcggcagtga ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact
     1621 ttaaaagaaa gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct
     1681 gccttatttc tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg
     1741 accttgtcat gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa
     1801 gtccaatatc tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg
     1861 ccgtttacgc agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt
     1921 ctagttgcta gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta
     1981 tctacactta ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc
     2041 gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat
     2101 tatttaacgg ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg
     2161 ttatgacaag atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc
     2221 attaagtcaa attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt
     2281 ctatcaaaaa attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct
     2341 ttccttgaaa gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg
     2401 tacaataaaa gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag
     2461 gttcaaagat atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct
     2521 ggcagaaact ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa
     2581 catccttgat gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagcaaa
     2641 ggcgactgca aagaaaatag acaaagtcta tgaagaggaa ccttttgcag cattagctat
     2701 ttatctatat gcagcttctc aaagttggaa aaaaatttat caaccattga agattccaag
     2761 aactatctat ttggcaacaa tgaatgcttt tactagattt attcatgaac atttccaagt
     2821 aacaggaagc tatcgctttg accgtggttt ttggatttgg cattacacta gtggcttgat
     2881 ctttcggatt ggtgaattgg agtacgaaat gacatatttt ccgaaagggt acacggatct
     2941 aagattagaa ggaaagcagt gcctttctct tcatattcct tccgatgcag atttatctca
     3001 gaataagata agagatagtt acgaaagtgc cattcgtttt tttgaacttt attttcctga
     3061 ttaccattac caagtgatgt acacagatac ttggttacta tcacccaatc ttactaaatg
     3121 gttgaaaaaa gagtcaaaaa tctgtctttt tgcagccgat tatcgattat tatcagttga
     3181 tgaacaggac gatagtggag tcccttggat ctttggacga gtagatgccc aaatacatga
     3241 ttatccagaa agtacgagtt tacagcgaca agcaaaagag caattgcttg caggcgagca
     3301 cattggctct ggcttaggta ttcttacttc ataagtttag tacttcgaaa tagtttaaaa
     3361 gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
     3421 gaagcagaag caactccttc ggaaataagc tgaaattcac aaaaatttaa aaagcaattt
     3481 tcttgagttt cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttcctt
     3541 ccttcaattt tttacacgtc gacgattcaa taaaaaaccg cctaatattg taaagacagg
     3601 tgcaagaatc gagaaaaagg cgaatggcaa gtaatgcaac gcgctaactt tcatcgttcc
     3661 cataatgaat gtgccgctga ctccccacgg tacgagagaa ttgacggtcg cacctgaatc
     3721 cgctagtgtt cgtgtcaaat aggtttttga taagccaacg tcatcaaaag aacgcttgaa
     3781 tgtttctcct ggaagaatga tggacagata ctgttctcca acgatcagat tgattccgat
     3841 acagctcaag gcagtaaacc cgactaaacg agaaggtcgg tccatctttt cttttaattc
     3901 ttgaacgatg gtatcaacga tcagatattt gatcagtaac ccacctaatg ccaatgcaag
     3961 taaaatcaaa ctagcagaac ctaacatact catgatcccg cctcgagaaa gcagttcatc
     4021 tagctttgga tcaggtgtat ttgcgacata accattcatc aagatgtctg ctgcttttcc
     4081 gaccgtcaaa cttttctcat ggataaaaga aagaacgaga gcagctgtcg atcccgctag
     4141 taatgtaggg atagccggaa tttttttgat tgccccccca aataaaataa ctagtggaat
     4201 caatgtccaa ggagagatcc aaaagctcgc tttcatgttt tggatcattt cattgatact
     4261 agctagatcg gctgaatgac tgttatgtcc taatatcgta taaaaaatca acgaaaggac
     4321 gaatgctggg aaatccgtat aacgcatacc agcgatatgt tcgaatagat taactcctcc
     4381 aatacccgct gccaagttgg tcgtatctga taaaggcgag cagttattcc ctaaaaaggc
     4441 accggaaacg actgcgcctg tagtcattgc atcattgaat ccaagtaaat gaccgatccc
     4501 caaaaaagca attccaatcg tcgaaatcgt tgtaaatgaa ctgccgacag tcactccgac
     4561 aattgcacaa acaagaaatg ctgtcggtaa aaagaaacgc acagaaacaa gtttaaatcc
     4621 ataaaccata atcgtaggga tggttccaga taaaatccat gaagcaacta agacaccaat
     4681 caaaagaaaa atcaggattg gaatgatccc tggagtgatt cctttgatga tcccgtcatg
     4741 gatctcatcc catgtgaact ttttaaatct gccataaaac aacaaacaca tgaaagctgc
     4801 aaggatcggg atatgtggcg agagctggaa tccgatgatc agtactccta ggagtgccaa
     4861 caacacgatc aatacagcga tactttcttt taaagaaata tttttattca tcgtcgatct
     4921 cctatttcaa atgaaaaggc cctgccatga aaaaactatt cacttggcag gacctcctac
     4981 taaatcagcg acaaaacgtc ggctgaaaga cttatttttt tatttttctt ctaaactttt
     5041 ttctacctca atcccccaag aatctttctt gaagtgattg atgatcaatg gaataacgaa
     5101 catgaccact gtgatagctg ctactaataa tgcatcactc aatgtcgctg tacctaatgt
     5161 agcaaatacg acaacgatga tagagaagat cagcatgcag ctgactaccc aagctaatcc
     5221 gtttccgttt ttaccaattc ggtaaggacg ttcgaattct ggatgtttct tacgcatttt
     5281 aagcaatgaa acagcaatca aaatgtacac gatacaataa ataacagttg tagcatttgt
     5341 aagtgtcaag aatacagcac ttacgtcatc cattacacca taaagcaaag caaataatga
     5401 aataacgatt gattgtgtta gaacgacatt tctagatacg ccgtatttgt tttctttatg
     5461 gaatccgaat tttggtggaa ggaatccttc acgagctact tggatgattg tttttgatgg
     5521 tccagtaacc caagctgata attgtaaaag cacaccaata aataccatga agctgaagat
     5581 atttccgatc acttctggta agcctaggat ttgacagtca atcaagatag gctgcgtgat
     5641 atttgctaat tccatttttc catctggtac gacatttgct acaagcatcg cattgatgac
     5701 attcaataaa actaatccga tcaaagcgat aaatacgcct tttgtataat tttttgttgc
     5761 gtctttcaaa cgaggcatgt aaacagaact catttcgatc ccaacaaaaa taaatgtgat
     5821 accagctaag tatttcaatg aatcaatatg ttccaagtca gggaaagctt ttgaccatga
     5881 aaagtcacct agatagccgt ttgagactag tccgactttg aaggctgcca aaacacctaa
     5941 aacgaacatg atgactactg ggatatatac ccctagccat acaccgatgt tcccgccgac
     6001 ttttgccata tcgaatttca agttcaagat cgtgatgatc cagtagataa ctaaaataca
     6061 acctaaaacg aaccagtgat tattgccaag ttctacatta ccaaatgtgt ttcctaacaa
     6121 tggtcctaaa gtagaagcga ccattaccat acctgggaac atttgcaccc ataatagcca
     6181 tgcaacgaca aatccccatt tgctgcccag tgctgttttt acccataact gaggaccgcc
     6241 ttcttgagga agcatcgtgg aaagctcacc ggaaaccatt gatatcggac cggcaaaaaa
     6301 caaaactgca aaaattgaat aaaaaatcaa tgtccaaccg actgcggcta acgtaggaat
     6361 actccgaacc gttgcacaca gtgccattgt cagaccgata aaggtcccaa gacccatttt
     6421 acttacttca gatttactca ccgttgacac tctccttgat tttttggata aaataacggt
     6481 gtctttgaag tagtttcagc aataaacgaa acaattcagc gaatatttga ggggattctt
     6541 tgagtttatt gcttgaaggg tttcttcaaa gacaacctca aaacaaaggg atcgaagagc
     6601 ttaggtctcc ccttgctctt cagatccaaa catgcgtcag aaacagttag tattcaagct
     6661 gttttctaac atgattacct tgcttatttt gcttcgcttg ccaataattg gtctgcgtag
     6721 attttttcca atttttcttg aagagctgct ttgatttttt cagcatattc ttcaaagtgt
     6781 tcttctttgt tcatgtatgg tgtcattacg ctggcacgca atacagttac ttttccggca
     6841 cggttccatt cttcatctga gaatcctagt tggttcacaa attgtaatgg gctgttgcca
     6901 tagtctggga tagcaaagtc agtatgtgaa gttaagaact cattaccgta aatgcttcct
     6961 ttcacgtatg aagaataatc atatacgtca tggttcaatt tattcattgc aactaagtca
     7021 tcgttgcctt tttctttgaa tacatagtct accatattga aatctggata agttaatgga
     7081 tgaacttcga tttctttgtc tcctacttta aatgacaagt cattcaagaa gttgtagaaa
     7141 cgatgtgcac cttcgattga tgcacccatt aatttaccat atcctgtgac attcaaaggt
     7201 aagacatggt gtgctgccca aacgctggct gctgttgccc ctgcttttga accttccaag
     7261 atataagccc caagtaatgc tgggatatct gcgccttttt caaatacata tgtcgcaaag
     7321 taagagataa cgtctctcat gcggatatct ttgatgacga taccaccggc agaatatgga
     7381 acatagccca ttttatgtgg gtcaatcgtt acagattctg cttcttcgat tgctttatat
     7441 gcactgtgta cttcttctaa gatataatct ttgttttctg taaagacatt gtatttgtaa
     7501 tgaacatctt ttaaatcttc aaatggaatg aagttgttgt cttcgtctaa gaaaatcgca
     7561 cggccgtatc cgccataagc agcatctacg tgtaagtaga agtaaatacc gtctttttct
     7621 aatacgcgac gcaatgcaac gattttatcg ataccgtcga ttgctccttc ttctgttgat
     7681 cctacaacac cgactacacc taggattggt gttttttcag ctgctagacc acgaacgatt
     7741 ttttcaagtt cgttgatgtc catgcgatag ttgtggtcaa ctggcactgg aatcacttgg
     7801 tctaaaccga taccgatgat gtcggcagct tttagccatg aatagtgttt agtttgtgga
     7861 accaaccatt ttcctaattt ttctaaatgt tttccgctac gtgctgagtg agctttgatt
     7921 tcgtcgattt tttctgggac gctgtctaac agattcatga tttcttctgt tgacaagttc
     7981 attaattccc aatcgctttt tccagcaact agttcaggtg tcacttcttt cattgcaagc
     8041 ggcaatgatt tgatgttgcg ggcataccaa agtccttcta agtttgctaa agaaccatca
     8101 gcaacgatgt ggccccagcc atctttatag ctcattaatt ttgcaaattc cattcctact
     8161 tcttcttcca tttggcttgt tgcaggagaa gattcatagg caacgttatt accattccat
     8221 agcatagcaa aattgtacgc tagtaaagat ggcatcaatg tttcagagtt catatgaccc
     8281 cagtaacgtc cggcattatg ccaaggaacg gaatgggtac gcatccgcgc tgaaatttct
     8341 gatagaacat cctttgtttt attcacagtg tgttcaaaac tagcactgct tttttcttct
     8401 ggcgtaataa taggcatgtc ttgaggcatg tagttttgac gccagcctaa atgttcatcc
     8461 accaattcat tcaacaaagc tttatagatt tggccgttct cagctttgtc cccaatgaat
     8521 agggcattta ggtttagatc tttcgacaat gattcactca tagtgaaacc ctcctaaaaa
     8581 aatatttaaa ttttgtcgag ccctacatta cacctgttct tctattctgt aaactaagtt
     8641 ttggcctgat ttttaatcat attttaatat gtatcaatta tccgaattgt tcacactttt
     8701 gaaataatta aaaaaaacga gggaatttac aaatgtaaac tccctcgcaa agattgtatt
     8761 aacgacaacc agcgttctat tttaggatgg gtttcaaaat tggaatgttg taatagtatt
     8821 tccctttttc aaggtaaaaa cttactaaat tctaatcaat tactttggcc aaaaagtaat
     8881 ttttcttacc ttttcgaata accacaaact taccatcgaa ttcatcggat ggatttatga
     8941 caaaatttaa atcggtaacg cggtcaccgt taatacttat agctccgttt gaaacatctt
     9001 cacgtgcttg acgtttagat ggttcaattt ttgtggaaac aagcagttcc acgatatttt
     9061 caggtgtgct tgaaatttca acgttcggca ttttaccgaa tccttgcgcg atttcttccg
     9121 catttagttc tttaatattt ccagagaaca atgcttgcgt tattttttgc gcttctttca
     9181 agtcttcttc actatgcaca aatcttgtaa cttcttctgc caaacggcgt tgtgcttcac
     9241 gtttctcagg ttctgtctct acttttttcg ctaagtcttc gatttcttct tgagacaaga
     9301 aagtaaagaa tttcaagtat ttcactacat cgcgatcatc ttgattcaac cagaattggt
     9361 agaactcaaa tggtgaagtt ttcttcggat caagccaaac ggcaccgcct gcagtttttc
     9421 cgaatttcgt tccgtcagcc ttcagcatca atgggatcgt taaaccgaac gcttctgctt
     9481 ctggtccttc taattttcgg attaggtcta atccagctgt gatatttccc cattggtctg
     9541 ctccaccgat ttgcaattgc acatcgtatg ttttatgcag atgcaaaaag tcgatagatt
     9601 gcaggatttg gtaagtaaac tctgtaaatg agattcctac ttccaaacgg ctggcaacga
     9661 tatcttttgc taacatcgtg ttgatgttga agtttttacc gtagtctctc aaaaattcaa
     9721 gtaaagagat ttttgacaac cagtcgtagt tgttcacaaa tgtgatattt gcatctttac
     9781 caaacaattt tctcatttga ttggaaagtg cgtccacgtt atgttgcact tgttccatcg
     9841 tttgcaacac acgttctgtt ttgcggccgc ttgggtcgcc gatcgttcct gttccgccgc
     9901 caatcagaat atatggatga tggcctgcca attggaatct tttcatcatc ataaatggaa
     9961 tcaaatgtcc gatatgcatc gaatcaccag ttggatcaac gccgcaataa agtgaaatgc
    10021 tcttttcttc aacgagctcg cgaagtcttt cttcgtttgt ttgctgatta atggcgtcgc
    10081 gccaagttaa ttcatcaata atattcattt tactgacctc cgtgtagttt ttttatgaaa
    10141 gcaggaacct gctagcatgt gtaattaatt atttcttcag gataggatag gaaataaaaa
    10201 aagtcccttg gcatataagc caagggacga aatctcgcgg taccacccaa tttgtatgtc
    10261 tagatatcca acatacctct cgaacattta acgctgtcgc gtggcaatca ctgccaaaga
    10321 ctcctgaaat gtaattcgct ttaaagcatg tactggtttg cagcgaccac cagctttctg
    10381 aaacattggc tttaacaact acttcgtttc gatcttcgtc ttgttattat taatatgtgt
    10441 ctatcataga gcgcaacttt ttctttgtca acagaaaaag gatatttttt ttaaattcct
    10501 cattttttct tttttgaatc cgctctaatt ccgaacattt tattttcaat tattgttaaa
    10561 agaaataact ttttctgtat tttttctgca atttatcgag aagtcacttt actttgcctc
    10621 ttattctttc ttcgttacaa taaggaatgg gacaagtaaa aaaatgaaaa agcaagggac
    10681 gaggtatatg gaaaaaaagc aaaaaattat tttaggagcc acttctgctt taactatcgt
    10741 tattttaatt ctaagtttgt tttttgtcat ccgatcatct acaaaaaaag aaccaatcat
    10801 tcctgaaaaa aaaataacag aaaaagcaaa agaaccgaca gatgataagc aacaactagt
    10861 tactcaagca aaaaatctgg ctcaaagcta cgattatgac caagcgattg cgttattgga
    10921 aaaagatagt aagaacggat ctactgaaac acaacagctt cttcaaacac taaaaaagga
    10981 gaaagaacaa ctgatcgact ggtctgatcc tactcagatc tctcatgtct ttttccacag
    11041 cttgatcgtg gacccgcaaa aagccttcca ctctcaacag gcacaaggat acaaagatta
    11101 tatggtgact gttgaagaat tcaaccgttc gatcgaacaa ctttatcaaa atgattttgt
    11161 tttagttaat ttgaatgatt tgatccaaaa agacgaacaa ggaaatcttt cctttaacgg
    11221 gcttgcgcta cctgaaggaa aaaaaccatt ggttctttca caagatgatg tcagctatta
    11281 tgaatatatg gataattccg gttttcctag ccgattggtc gtatcaaata acaatgaaat
    11341 caaaaatgtc tataaagaga aagataaaga agaaatcggt gactttgaca tggttcctct
    11401 gatcgatgcc tttgtcaaga aacatcctga tttttcttat cgaggcgcaa aaggtacctt
    11461 ggccctgaca ggttacaacg gagttttagg ttatcgaaca tcaaaatctc aatatggaga
    11521 caatgaaaaa acaaaaaaag agattaaaga agctaaaaaa gtggcggaac agttgaagaa
    11581 agacggctgg agctttgctt cccatacatg gggtcacttg aatatgactc aagcttcact
    11641 agaagatgta aaaaaagaca atgaattgtg gcagcaagaa gttgcaccta tcctaggaaa
    11701 aacaaatatt ctgatttatc catttggtgc agacatcagc gactggcagc cttattcagc
    11761 agaaaatcaa aaatttgctt acttgaagag tcaaggtttc gatattttct gtaatgtcga
    11821 tgcttctaca ccagcttggg gacaattagg tacggatttc tatcgaaatg ctcggatcaa
    11881 tatcgatggc attcgttttc aatctgattt agacggaaaa aatcctattc ttgatccatt
    11941 catcaatgtc catgatgtct atgacaaaga agcacgcgaa tctgcttgat tttacgtcat
    12001 ttatgaacca aaaaagccga actgacattt tatcgtcagt ttcggctttt ttatgcttta
    12061 tttcttcttc ggtgcgattc ccttgatcag gacttctgtc cattctgctg tatatggaag
    12121 cagttttgcg tatatctccg gcttagaaat tccttctacc attgcttgaa aatagaggat
    12181 caagaactca tccgaatatt tcaaatccac ttttccttct ttccgccctc gatggaaaag
    12241 atcaagcatg atactgaaac tttcttgcgt atacgcatcc atcgcgtcag aaaattcttt
    12301 tttttctttt tttgtatagt atgtcatcaa atcgatataa aattgtttgt tcactcgatt
    12361 gagataacgg attttgtttt cactcatggc aatcagtgtt tcttcaaatg gaagattttc
    12421 cgcaatcaac gctttcgctt cttgccctaa atcattcata aatgcaaaaa atacttcata
    12481 aatcagccct tccttgctgc caaaatattt aaaaatcgtc gctttactta cctgagcgtt
    12541 ctcagctacc ttatccattg tcactccagc ggttcctatg tctgtgttca tcaagtcaaa
    12601 agctgcttgt aaaatctctt tctttttctc ttgtgctctc ttttcaaaac cgttcatttg
    12661 tagagcctcc cg
//
LOCUS       DAZKNO010000077        12511 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000077 DAZKNO010000000
VERSION     DAZKNO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.078"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(225..2300)
                     /gene="glyS"
                     /locus_tag="ACG8MY_001600"
     CDS             complement(225..2300)
                     /gene="glyS"
                     /locus_tag="ACG8MY_001600"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0430791.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2302..3219)
                     /gene="glyQ"
                     /locus_tag="ACG8MY_001601"
     CDS             complement(2302..3219)
                     /gene="glyQ"
                     /locus_tag="ACG8MY_001601"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0430792.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3605..4417)
                     /gene="recO"
                     /locus_tag="ACG8MY_001602"
     CDS             complement(3605..4417)
                     /gene="recO"
                     /locus_tag="ACG8MY_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0430793.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(4560..5459)
                     /gene="era"
                     /locus_tag="ACG8MY_001603"
     CDS             complement(4560..5459)
                     /gene="era"
                     /locus_tag="ACG8MY_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0430794.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5474..5869)
                     /locus_tag="ACG8MY_001604"
     CDS             complement(5474..5869)
                     /locus_tag="ACG8MY_001604"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0430795.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(5853..6329)
                     /gene="ybeY"
                     /locus_tag="ACG8MY_001605"
     CDS             complement(5853..6329)
                     /gene="ybeY"
                     /locus_tag="ACG8MY_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0430796.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6346..8538)
                     /locus_tag="ACG8MY_001606"
     CDS             complement(6346..8538)
                     /locus_tag="ACG8MY_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0430797.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8562..9533)
                     /locus_tag="ACG8MY_001607"
     CDS             complement(8562..9533)
                     /locus_tag="ACG8MY_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0430798.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10239..11618
                     /gene="brnQ"
                     /locus_tag="ACG8MY_001608"
     CDS             10239..11618
                     /gene="brnQ"
                     /locus_tag="ACG8MY_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0430799.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11774..12220)
                     /locus_tag="ACG8MY_001609"
     CDS             complement(11774..12220)
                     /locus_tag="ACG8MY_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0430800.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12247..12423)
                     /gene="rpsU"
                     /locus_tag="ACG8MY_001610"
     CDS             complement(12247..12423)
                     /gene="rpsU"
                     /locus_tag="ACG8MY_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0430801.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
BASE COUNT         3394 a         2669 c         2279 g         4169 t
ORIGIN      
        1 actagggaaa ttcctaattt aaaagtaaat aaaacagtaa caagatcatg attaatcgtt
       61 ttcattttta tttcctctct tttcatgagc attttgaaaa ttcgaacaaa gaataaaata
      121 acaaaaatta ttaaaataga attaataaca gctatttata tttataacaa aaaaacagag
      181 acacagcacg tttcgtgcat gctctctgtt tttcaaacaa aagactattt tgtgatgatt
      241 tgatccaagc ttgctaatga caaagccatg gcagctattt gactaagttg tgttagtcgg
      301 ttcttacgta ctgctggttc atcagccatg accatcgttt gatcaaaata attttcgatc
      361 agtggacgaa gagcgacaag ctggtcgtag ttttcgtgga tcgtttggtc agcaaaactt
      421 gctttcattg cttctactgc tttgtgtaat tctttctctg cgtcgttttc gaacagctca
      481 ggatctactt ctgattgttt ttcaagctgc gcttttttcg ctaagttcac gacacgtgtc
      541 aaagcttcca ttgaaggttt gaagtcttca tctgcaagat gttgtttgaa caattgtgct
      601 gctgcaaata cttttgtcag atctttttgt tccgcattca aaacagcatc gatgacatca
      661 tgacgaatat tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcact
      721 tgttgttgcc cttctgataa accaatgccg aaacgatccg tatctttact gataacatcg
      781 ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgatccga
      841 acgacaccgt atgcttggcg acgtaaggca taaggatcgt ttgaaccgct tgggatcatc
      901 ccaacagcaa agaaagaaaa gatgctatct aatttatctg caatcgctaa aacagaacct
      961 actgtagact gaggtagctc tccttcactg gatataggca tgtaatgctc acgaatcgct
     1021 gtcgctacag caggtttctc gcctttcagc agagcatatt tttcgcccat gatcccttgc
     1081 agttcaggaa attcccctac catgttggtt actagatcaa atttatagat ttcagcagct
     1141 cgttgcagat ccatcaattc ttcctcagat agtcctactt gttcgccaat gatacgggca
     1201 atgacttgta cccgttgcat tttctcatag atactaccga ttttttcatg gaaagtaaca
     1261 gtttttagtt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat
     1321 tccgcatctt ctaaacgagc cgtcaatact ttttgattgc ctcgaaccac attttccaat
     1381 tttacccgat caccattacg agcagaaata aaatgaggaa gcagcatacc ttgatcattc
     1441 cgtacctcga aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca
     1501 gaaagatatt tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg
     1561 acttcttcta gtaaatcttc gtctagatca ataacccaat gatttttttc ggctaattca
     1621 ttggcttgtg taacgatcat ttcttgtctt ttttgcggag acgcaatgac gaattgttca
     1681 ttcaattttt cttcatattc ttttgcatgc gcaaatgtca catcttcgcc taagaaacga
     1741 tgacctcgag aggctctgcc tgttttgaca tccaagattt cgaatgggat tacttcgtca
     1801 tctaacaatg ccacgatcca gtgaataggg cggatatatt caaaatcgta ttctgcccaa
     1861 tgcatcgtta cagggaaagt caggctttta atgacatctg ctaaacctgt caatacttct
     1921 ttggattcct taccatgagt gtatttcgtt acatagacat attccacacc ttttaattct
     1981 ctaaaagtga tagcgtctgt cgtcagacct tgtccgcgga caaatccttc cgctgctttc
     2041 gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttca
     2101 ctgtccgctt gtcgttctgg aatgtttgtt actttgattg ctaaacggcg cggtgtggaa
     2161 aatgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct
     2221 tctaattgtt tcatgcttgg tgtgacaaca tgagcaggca tttcttccag cccgatttca
     2281 agtaataagt cttttgccat cttattcagc ctccttcacg ttttctggat ttgctgtgtt
     2341 tttattcaac aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc
     2401 acgagccatg ttgcggatgc gagaaagata accggcacgc tctgttacgg atactgcacc
     2461 gcgggcgtct aataagttga atgtatggct acatttcaag atataatcat acgccggatg
     2521 aacaagattt tcatcgatac agcgtttagc ttctttttca aaacgatcaa agttgcttag
     2581 tagcaagtct tggtcgctta cttcaaaaga atatttagaa tgttcatatt ctggttgttt
     2641 gaagatctcg ccatatttca cgccatcagc ccattctaaa tcatagacac tttcaacttc
     2701 ttggatataa gaagcaagac gttctaatcc ataagtgatc tcagaagtta ctggtttaca
     2761 aggcagtcca ccaacttgtt ggaagtacgt gaattgtgtg atttccattc cgtcaagcca
     2821 tacttcccaa cccacaccgg cacagcccat ggatgggttc tcccagttgt cttcaacaaa
     2881 acggatgtcg tgttctagtg gatcgattcc taaaaggcgc aagctttcta aataaagctc
     2941 ttggatattc tcaggtgaag gtttcatcac gacttggaat tgatgatgct gatacaaacg
     3001 gtttgggttt tcgccataac gtccatcagc tggtcggcga gatggctcta cgtaagcagc
     3061 attccatggt tctggtccga ttgcccgtaa aaaggtatat gggctcatcg tacctgctcc
     3121 tttttctgtg tcatacgctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa
     3181 tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt
     3241 taaagcgttg ctgtcaaata agaatacagg aaagaaaacg acaaaaaatc cctatgtctg
     3301 attacagaca tagggacgaa taattcgcgg ttccacccta cttccgaata aagattcggc
     3361 agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggctcccact
     3421 ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt
     3481 tgcagttttc ttgtttctat aatagatcct ctcagtgaac gatctatttg atctacggtt
     3541 aataatagac ggaactccct gtgtttgtca aatggttttg attttttgtt gtatgtttca
     3601 tttactattc ttctttttta ggtagatctt tttcagacat gtctttctct gacgtcttta
     3661 atacgtgtgc ccaagacttc atttgatcaa tgaacttttt gcttttcaaa tggataccga
     3721 catattcatc atacagctgg tcgatgagtt cacggattgc ttgtttggtt tcttctttca
     3781 gatggattcc actgatttta tcataagaaa tagctgaaaa catccgaacg aaataaacag
     3841 ctcgaggatc tgcatgataa cggtgatcat ccatcgacca gtgtctttcg catatcacac
     3901 caccatattt ggaagaatag tcaaattttc cttttgtttc cccacatacg acgcagtgtg
     3961 tccagattgg tgcaattccg aagcgttgca gtatttggac ttcaaaaata ttcgtgatga
     4021 tttctgcatc atctccttgg tccatcagtt gcagtgcttg ttgcgtaaac tggaacaaat
     4081 gcgggtcgta ctgatgatct tcgattgccg catctactag attcaataaa tatgtgccat
     4141 agccgctcaa aaagatgtct tcttggatgt gtcgaaacgc gtggacatct ttgacactgc
     4201 ttaaaaatga caaaccttca cttcgaaaat tcccgatata aactgcttca gtaaaaggga
     4261 gcaaagccgg agctaagggc tgattttgcc gatggatccc tttgatataa aacatcattt
     4321 tgccagaaga ttccgtaaag attttgacta atttatcttt ttctttgtaa ttacggctag
     4381 ataaaatcaa gccttttgat tctgcaagct ggctcatatt ctccctccta aaaaataaat
     4441 ctggaaaagc agattctgcc gatccagatc atttatgttg cgatcttccg cagcttttct
     4501 caacacgcgg atttttatcc agtttatttc tatacctaac ttacaagaag ctcagaatat
     4561 caatattcgt cttttcggta gccgaagtct tgcagataga cttttttgtc tcgccagtct
     4621 ttttgaactt tgacccaaag ttctagaaag actttgtctc ctaaaagatt ttcgatatct
     4681 tttctcgctt tcgttcctac ttgcttcagc atttttccgc ctttgccgat aatgatccct
     4741 ttttggctat ctctttcgac aataatcgta gcctggatat ggattttatc attctcgttt
     4801 cgcttcatcg aatcaacaac gactgcgaca gaatgaggca cttcgtctcg tgttaaaaac
     4861 aagactttct cacggatcaa ttcagaaacg atgaagtatt caggatgatc agtcacttga
     4921 tcatctggaa aatattgtgg gccctctggc atttcactga ctaatacatc catcaaacgt
     4981 tcgacgttgt ttccttctgt agcagaaatt ggaatcactt gtgcaaacgg catctgcgtt
     5041 gaataatctt cgatgattcc caacaactcg tctggatgga ttttatcgat tttattgata
     5101 atcagataaa caggagaatg cacgtttttt agacgttcaa taataaaatc gtctccccga
     5161 ccgcgttttt gatcagcgct gatcataaat aagaccgcgt ccacttcttt caacgcactg
     5221 taagctgctt ctaccataaa atctcctaaa cgatgctttg gtttatggat tcctggtgtg
     5281 tcaataaaaa tcaactgagc ttctggtgta gtatatactc cttggatttt atttctagtt
     5341 gtctgcgcct tatcactcat gatggcaatt ttttgaccca cgattcgatt caataatgtg
     5401 gattttccta cattcgggcg gccgacgatt gcgacaaacc ctgatttatg ttctggcata
     5461 tacgttctcc tttttaatta aacaacaaat gatatatttt agggacaaat aaaatcaacc
     5521 caacgatgac ggaaaaacaa gcggtcacta aaactgctcc agctgccata tctttgattt
     5581 ttttcccaat cggatgaaaa tgaaaatccg taaacatgtc cacgacattt tcaaaaacag
     5641 tattgatgat ctctactatc catacgagaa aaacagctaa aagcagccat aaccattcta
     5701 tccgatcaag ctggaaaatc acgcccataa acaaagcgag gattccgaac gcaacatgtt
     5761 tcctcatgtt gcgttcttcc tcaaaaacgg ttttgactcc ttggacggca aattccacgg
     5821 acatcaggaa atgtttgttt ttctctacgt ttttatcttt taagtccata ggcatctaag
     5881 atctctttct gtaaaccaaa catttctttc tcatcctctg gtgtcatatg gtcatacccg
     5941 ttgatatgca agaatccatg aaccgctaaa aagccaagtt cccgatcata cgaatggccg
     6001 taagcttctg cttgctctgc agcacgttca gtagagatca tgatgtctcc cagtacacga
     6061 ggcaatggat tctctccatc ttcaaaaatc actgggattt cgtcttcgcc ctcttcttcc
     6121 aaggcaaaac tgatgacgtc tgtcggtgca tcttttccac ggtaatcacg attgattact
     6181 tggatcgctg cattgtccat gaaagtaaca gacatttctg tttcttctgg caactctaaa
     6241 tatgatgctg caaattgcag caagtcgtcg atttctttca ttttctcttc tgagacttgg
     6301 tttgtttcat ccatgaaagt aatatccatt atacatcgct tctttctatt cttcttgttc
     6361 ttcttttatc ttctcaactt ccgatttcat tttaggatat ttgatccgag aatggaaggt
     6421 actgcttaaa ccgctgatca atgatttttc tactttacgg atttctttta acgtcaatcc
     6481 actgtcgtcc aattgtccat cggaaatacg ctcttcaata agattatgga caaaactttc
     6541 gattttttcg ctcgttggat ggtccattgc tcgtactgcc gcttcacaac tgtcagctat
     6601 attgacgatt cctgcttcac ggctttgagg ttttggtcca ggataacgga attgctcttc
     6661 cgtgacttcc gggttgcgtt cctttgcctt aacgtagaaa taacgcatca gcgtggttcc
     6721 atgatgctgt ctgcaaatat cgatcaccat ttgcggcata ttataatctt ccaatatttt
     6781 ggcaccatct gtcacatgtc caaagataat atgtttactg tcttctggca ataagaaatt
     6841 gtgcggattt tccgctcctg aaggcaagtt ctcaacgaaa aagctggcgt gtttgatctt
     6901 gccgatatca tgataatagc aagctactcg agttagtaaa gaacgtccac cgatttctgc
     6961 tacagcatta gcacttagat tggctaccat catgctatgg tgatacgtgc ctggtgcttc
     7021 ttccagcaac tgtttcaata aaggatgatt aggattgctg agttcattta atacgatcac
     7081 actatcatca ttgaccatca attcaatata ggggtgcaga cctatcgtca gcaagaatga
     7141 gaacagtgca cctgtgaatc cgcatactag cgttaaccaa gtcgttgcat ccccaaagct
     7201 cattccttgg tagataatca aaatgacatc catcatcaca ggaaaaataa tgacccacat
     7261 agcagcggat aacccttgtt ctgagatccg tttgcgtctg acgatcgttg ccatgagacc
     7321 agaaaataca taggacatca aaataactgt cagaaagttc gtgccaatcg aatcataaaa
     7381 aatgaaaagt gcggatactg cttgaaaaat accagccatg atccctgctc ttcgattcaa
     7441 gaaaaagctt aaaacaagtg gagcaaaagc cgcggggtaa aacagcggaa tataagcagc
     7501 ttgttctgtt tggaagagtt ggaaaaattt cattagtacg acgcttactg acattgccgt
     7561 cacgtagaaa agtacgtatt ttgttctttc tcctgcattt tggaattgaa gtgaattata
     7621 aatcagtaca ccgatttgaa gcaaaacagc taaaaccatg gcaatgagtg ggaaaataga
     7681 agtcgtttga ttcgtcaatc ctagtaattc caattttttc atcgctgaag catcaatttg
     7741 atttccttcg cgaacaataa tttctccttg gaagatcatc actggttgta cagcttctcg
     7801 tgcagattgt tttaattctt ctgtcttttt ctcattcaat acatcattaa ctacaatgcc
     7861 ttgatccact aagtagcgaa tcatttgttg ctgctctgtt gtgacattca aatattgaat
     7921 ctgatcttct gcttcttgtt taaaagcttc caattcgctt tcacgaactt gtttactcat
     7981 ctgttcgtca atgagcttca aactttcatc cctaaccgtg tgaatttccg tttcagtcat
     8041 ttcaaatatc gtcgaataaa aattgttcgg cagtttttgg taaaacgcaa cgttttctgc
     8101 attcacgttt tcaaaatttt tcttcagtgt agcaatccgc tcttctgtag taggtttcgg
     8161 gatattttca tcttccttcg ccttatctac cttgtcatta taagatttgt tgatggtatc
     8221 attcgtctta tcgatcaatt cgaataattg cttgatccga ttgttctggt catctgccag
     8281 atctttttga taagtatatt ctggagtcac tgcttctgct gccagttttc ttctttgttc
     8341 ggtttcttcc gtgttctcaa tcgttttatt cgcacggata ctctcttctg ctacttggcc
     8401 ttctctgaaa tcattggcct tttgtttgac actactaaat gtaacgccgc agacaatgat
     8461 aaaaaacaag aacagaagga aaggcaatgc tgcctttccg agtttttttt gtagtttctg
     8521 cagtgtttta ttcaacatgt gcttatcctc ctaagcaaag cttattcttg atgaagatcc
     8581 gccttttcat aagctttgat gatttcggca actacagggt gccgaacaac gtcacttgct
     8641 tcaaaattaa caaaatcgat ttttttgatt tgctttaatg ttcgttctgc atgaacaaga
     8701 ccacttgtcg tcccacgagg caagtctatc tgacttgtat ccccattgac aatcattttt
     8761 gattgataac cgagacgggt caagaacatt ttcatctgag cgactgtcgt gttttgtgct
     8821 tcgtccaaga taacgaatgc atcatccaat gtccgccccc gcatgtaagc taaaggtgcg
     8881 atttcgatca cgcctcgttc catcaatcgg ttcgtatgat ccaatccaaa aatttggtat
     8941 aaagcatcat aaacaggccg cagataagga tccacttttt ctttcagatc cccgggtaga
     9001 aagcctaggt tttcccctgc ctccacagcg ggccgcgtca aaatgatttt ttgcacttcg
     9061 ccttttttta gggcagcaat tgctaatact acggcaagaa acgttttccc tgttccggct
     9121 ggccctacac caaatacgat gtcatgtttg gctaccgatt caacgtattt cttttgaccg
     9181 ctgtttttta cccgtatagg ttttccatta cggtctttga cgatctcttc ctcatacatc
     9241 tcaacaaaat aatctaatgt acctttttgc cccatcttca atgccgtcac aacatctggc
     9301 gtacttacgt ggattcctcg tttgatcaat tcctgtaatg tccgaatgac tgaggcagcc
     9361 aaggaaatct gggattgctc accaatgatc tgaatgattt ctccacgtgt atggatggtt
     9421 gtttccgttg tttcctcgat cagttttata tgcttgtcgt gtgcacctaa aagcatgctg
     9481 acatcatcag cagctgtcaa tctaatctct aaagaactag actcttccgt caaaaataga
     9541 catccccttt ttttcgattc attctttcct attttaccat actcagaaga aactatatag
     9601 caaataagaa aaggttgtaa aagagtcgtt taaccatacg taccaagaaa cgttaaaaaa
     9661 tagccgtttc cattcgctaa aggtctcgtt ttgataaaaa gaagctgaga atgtaagtta
     9721 tatgaaagtg gagaacaaaa tattccgttt cattcagcag gtattctcta ggtatttttc
     9781 ttgtttcaaa aaaagaaaac aaaatagtta aaatattctt gcatttcaaa aaaaacaggt
     9841 gtattattac gtcaacaaat aaacatacag caaagcgtcg aggaaaacag taggctattc
     9901 aacgatttat tcccagtgag tcatgtaagt gagaaatgac aataaatcga atagacgaac
     9961 atcatttccg agtcaatgtc gaaaacgtag atcattgtgg ctgcctccat tatcaggcta
    10021 gcggataact ttatcaaaaa gcgtccgtga atgaagaagc ttttttaagc gaaaaaaggt
    10081 ggtaccgttc tagaatctag agcccttttc ccaagtggaa aagggctttt tttatatttt
    10141 aaggtggtgg ttttcatgga atctgatatt agtggcatta ctaattgctg caacgtttat
    10201 atcgtcaatc aaaatgataa ttattggagg aaaaatttat ggaagaaaca aaaacttatt
    10261 catggaaaca actattgatg gtcagttcac ttatcttcgg gatgttcttc gggtcaggta
    10321 atctaatctt tcctgtccac ttaggtcaaa tggctggcgc aaactgggtc agtgctggcg
    10381 taggatttgc catctcgggc gctcttttcc cattactagc tatcttagct gtagtagtga
    10441 caaaaagtga tggtctgtat gacttagcta aacctgtcgg aaaatggtat gctgccttgt
    10501 tcttaatcct tgttcatttg acgatcggac catttttcgg tacaccacgg acagctgcta
    10561 ctgcttttga aatggctgct aaaccatttt tgcctgaaaa atataattca ttaggtatgt
    10621 ttttattctc tgctcttttc tttttacttg cttatttcct tactgtccat cccacacggt
    10681 tggtcaaata cgtcggaaag tatctaaact ccttgtttct tgcattgtta gcaatcattt
    10741 ttgtcgtttc tttattccac ccaatgggaa atctaaatca atctgctgca gatacttacc
    10801 aaacaaacgc tttaacaaat ggtgtcctag aaggttataa cacggttgat gcagtagcct
    10861 tgcttgcctt aagtgtcact tttgtccatg cagtccgtga ccttggtttc aaagataaaa
    10921 aagtcacaga attaacagca aaagctggta gcctgagcgt tgcgctagaa gtcatgatct
    10981 attttggtct tgttcttctc ggtgcgatga gtttgaataa gatgtcttta tcagaaaatg
    11041 gcggaactgc actttctcag atcactaatt tttatttagg taacttcggc gctagcttct
    11101 taggagtgat ggtcacatta ggtgtcttca ctaccgctat gggactcgtt gtttcttttg
    11161 cgcaagattt ccataaactg ttccctaaag tcagttatat gacatggctg cggttaacga
    11221 ctttcgtctc ttttgtcgtt gcaaatgctg gtttagacaa tatcatccaa tggtctttac
    11281 cagtattgat gcttttgtat ccattgtcgc ttgcgttgat cttgctttcc ttaacagcaa
    11341 aatttttcca aaagacacca tttgtctatc aagtaacgat gttgtttgct gctgttcctg
    11401 ccgtattgga tatgctggca aattcgcctg ctttagtgag tcaacaaaga gttgtagcaa
    11461 gcatgctcga attttatcat catcacgttc cttttgctgc attaggactt ggctggatgg
    11521 tcccaacgtt acttggttac gctggcagcc tactgtttta ttacgcctat cgcctatcag
    11581 ggtataaaca agaagcaaat gaactaccag aagaataacc aaatcaaaca caatttaaca
    11641 taagaaatat taaacaggaa aaaaccagct agatgaaaga atcctattct ttcgtctagc
    11701 tatttttttg ttctcttttt tacgcgcaca aaaaaactgc ggacaatgat tgtccgcagt
    11761 ttaaatgata aaattaagat tgtaatagtt ctttcacaat ggcatttact tggtttccat
    11821 ctgcttttcc tttgacttta ggcatgatcg cacccatgac tttgccaaat tctttcattg
    11881 atgaagcacc agtttgtgtg attgcttctt gaacaagttg tcgaatttct tcgtcggaaa
    11941 gttgttccgg catgtatttt tcgactatca caatttcact ttttactttt tcagctaaat
    12001 cgtcacgtcc tgcttcttca aactcatgca aagaatcacg tcgctgtttc atttcgcgag
    12061 acaacaccgt taattcttct tcttctgtca agtcgtgacc ttctttaatc tgctcgtttt
    12121 gtatagatga cttgatcatt cggatgactt gtaaagattc cttatctttc gctttcatcg
    12181 ctgttttcat gtcatcgttt aatctactta gtagtgacac actaccaact ccaattcatc
    12241 acagaactag aattttttgc gttttctagc tgcttcagat tttttcttgc gtcttacact
    12301 tggtttttcg tagaattcac gtttacgaga ttcttgtaaa gtacccgctt ttgacacgga
    12361 acgtttgaag cgacgaagag catcgtcaag tgattcgttt ttgcgaacga ctgtttttga
    12421 catgttaatt ccctccctcc gagcttaaaa agtaaccgta attgttattg aaatatatct
    12481 caaaaaacaa gactgtcctg taaatattat a
//
LOCUS       DAZKNO010000078        12396 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000078 DAZKNO010000000
VERSION     DAZKNO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.079"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1161)
                     /gene="rpoD"
                     /locus_tag="ACG8MY_001611"
     CDS             complement(52..1161)
                     /gene="rpoD"
                     /locus_tag="ACG8MY_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0430802.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1185..3026)
                     /gene="dnaG"
                     /locus_tag="ACG8MY_001612"
     CDS             complement(1185..3026)
                     /gene="dnaG"
                     /locus_tag="ACG8MY_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0430803.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3208..5523)
                     /locus_tag="ACG8MY_001613"
     CDS             complement(3208..5523)
                     /locus_tag="ACG8MY_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0430804.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5708..6568)
                     /locus_tag="ACG8MY_001614"
     CDS             complement(5708..6568)
                     /locus_tag="ACG8MY_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0430805.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6627..7259)
                     /locus_tag="ACG8MY_001615"
     CDS             complement(6627..7259)
                     /locus_tag="ACG8MY_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFQ0430806.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            7426..8187
                     /locus_tag="ACG8MY_001616"
     CDS             7426..8187
                     /locus_tag="ACG8MY_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0430807.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8213..10804
                     /gene="mprF"
                     /locus_tag="ACG8MY_001617"
     CDS             8213..10804
                     /gene="mprF"
                     /locus_tag="ACG8MY_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0430808.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10992..12179)
                     /gene="tuf"
                     /locus_tag="ACG8MY_001618"
     CDS             complement(10992..12179)
                     /gene="tuf"
                     /locus_tag="ACG8MY_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0430809.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT         3506 a         2629 c         2195 g         4066 t
ORIGIN      
        1 aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg cttattctaa
       61 gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg ctttagcctc
      121 gatctgacga atacgttccc gagtaacacc aaacactttc cctacttctt ccaatgttct
      181 cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc gatcagtcaa
      241 tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt gttctgctgg
      301 acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt cttcaccgat
      361 tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga ctttttctgt
      421 tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa ggtcttgcag
      481 caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa ctggaatacg
      541 gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc accatgtagc
      601 ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct tcatcagtcc
      661 catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat aacgtttagc
      721 gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt cttggtcgcc
      781 ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg agacacggcc
      841 aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg gcgcagaaag
      901 atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt gaattgatgg
      961 atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga tcaatttgtc
     1021 catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat catcatatac
     1081 aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt catacgtttt
     1141 tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt ggcttgtttt
     1201 aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg tttgtttcca
     1261 gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc taagccagat
     1321 ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc tgccaccggt
     1381 aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa gtctaaaaac
     1441 tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa ataaacttct
     1501 gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg gacagaaact
     1561 tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt caaaggtctc
     1621 tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg aggttcttga
     1681 cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt ctctctgccg
     1741 atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg tgaaacaact
     1801 cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc attatctaaa
     1861 tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg cccatgagcc
     1921 actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg atcaagcttt
     1981 tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa ttcaatccct
     2041 cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa ttctttcgtc
     2101 agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga agctaatcca
     2161 gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa caacaaaatt
     2221 tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac ttgtctttta
     2281 ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc ctgccctttt
     2341 tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt acgaagtgga
     2401 aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt gacaaacaat
     2461 ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg ctgaagaaaa
     2521 gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa agacaaccca
     2581 cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag catgtgatga
     2641 tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga ttcaggattt
     2701 tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc gaagtctgcc
     2761 acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa agtaaacacg
     2821 tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc aacagaaaaa
     2881 gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc agactttttc
     2941 aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac tgtttcgata
     3001 acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat atcttttggg
     3061 tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact gaaatcagtg
     3121 cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg ctttttcaag
     3181 tataacacat ttctatctca ttaagcatca atttgctact cttttcagta actgattttg
     3241 aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga atacaaagaa
     3301 gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga acagcattgc
     3361 agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg ggttgaaggg
     3421 ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat acatcaaagt
     3481 agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg caatgatatt
     3541 cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca aataacggta
     3601 agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc cttcaatcgc
     3661 taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg taacggcaca
     3721 tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg agactttcgt
     3781 cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag taaaatcaga
     3841 gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg ccatagcaat
     3901 gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg tcattgcaac
     3961 cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg gactcactcg
     4021 tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag taatttgaga
     4081 aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat tggagacagt
     4141 aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa gataagctag
     4201 tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa ccggctcaac
     4261 aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca taagtacgcg
     4321 atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt ccaccaaacg
     4381 ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc cccatttgcg
     4441 atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct caaaataatc
     4501 tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg tccccataat
     4561 ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga tactcatttt
     4621 cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg ccagcgtttc
     4681 aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta cccctgtcgt
     4741 taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca aagctgctgc
     4801 agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt ccaaaaatag
     4861 tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc gaatcgaatc
     4921 tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc ggacagcata
     4981 attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca aaaagcttcc
     5041 agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg tcaaaagagc
     5101 ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct gttctcctgc
     5161 tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt gttctttacc
     5221 gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta caagatttct
     5281 tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg ctaaatacgc
     5341 catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt tcagaaaatt
     5401 gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg catcttcctg
     5461 gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg taagtccatt
     5521 cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt taagtctagc
     5581 gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca cacgaatgat
     5641 tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt ttattatcaa
     5701 tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt gctgtaccgg
     5761 ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg taatcggtag
     5821 gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat agcttgctat
     5881 atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa agaatcccac
     5941 caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca gccatcgctt
     6001 tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg atcataccca
     6061 aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact tgcccgtcaa
     6121 ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca accatgatga
     6181 tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca ggaacagttc
     6241 cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct aataaaaaac
     6301 tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca ggcagaaaaa
     6361 acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc agccgctcat
     6421 acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata aatcctgaac
     6481 cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac cccacttctt
     6541 gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca tttaatttta
     6601 ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt ctctattatt
     6661 ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg atagagatag
     6721 ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc ttcctcattt
     6781 cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac tgtgttttct
     6841 ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc ttggacagct
     6901 gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa atatttgaca
     6961 ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg cagactttcc
     7021 gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat cgccttgatc
     7081 aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc tatttgatag
     7141 ctttccactc gttttaccga acgataagtt cttcctccta aaaacaacac cgcaccaata
     7201 accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt tcgcttcatt
     7261 tcatcaccta gttattttga catctttttt tatcttacta tttctttatc ttcttggcta
     7321 tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt attttttgac
     7381 atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac tgatataatc
     7441 gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc aaaaatgcca
     7501 gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt tttactgcat
     7561 ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga acgctactat
     7621 accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc atctatgttg
     7681 aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa aatcgatcag
     7741 gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc cagcagcttc
     7801 cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa aggcgttcgt
     7861 ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact ctctttattt
     7921 cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga catcgggctt
     7981 acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg aaaaaaagat
     8041 gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc ttcctttgtg
     8101 cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt caatcgagaa
     8161 gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt atttgttaaa
     8221 aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct ttttgatttc
     8281 tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat ctatccagca
     8341 attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc tattgattgg
     8401 tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga tgcttgaaca
     8461 aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga tcaacaatat
     8521 tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg gacaaaagaa
     8581 agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga tggctggact
     8641 ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg ctcctttttt
     8701 gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg tctttttatt
     8761 tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc aaggacagct
     8821 attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt ctgttggtta
     8881 tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg ctgcttcagt
     8941 gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa tgatgattat
     9001 cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct tgctctatcg
     9061 cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca aaaatatcgg
     9121 ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag aaattgcgca
     9181 taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt ctgccactat
     9241 cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga aatttcattt
     9301 tattatccaa tttcctagta tactattggg atttctttta atcattatgg gcagagggat
     9361 cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc tagctttact
     9421 ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta ttctgcttct
     9481 gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt catgggagtg
     9541 gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg ttattggggt
     9601 atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt tcttatttcc
     9661 ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt tcatgatcgt
     9721 tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct ttaatgaaga
     9781 aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat tgatcttctt
     9841 aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc ttctccaatt
     9901 cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga aagaagattt
     9961 tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt taccagtttt
    10021 ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg atttcattaa
    10081 gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga aaaaaatgaa
    10141 gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg atgtcttaca
    10201 accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata attggttggg
    10261 ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc tacaaagagc
    10321 gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa cgatcatgcc
    10381 tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt ctaaaaaagc
    10441 accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga aagaagaaca
    10501 tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt ccagaaagag
    10561 ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt tttattcttt
    10621 ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac gttatactct
    10681 atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta ttgataatgc
    10741 gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac gaagaaagta
    10801 ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga ctgaagtaaa
    10861 acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag agcggtccaa
    10921 acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc aaaatattcg
    10981 agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt ccgtccgcct
    11041 tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa gtcaacatca
    11101 atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt gattgttcct
    11161 gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa tggagtatga
    11221 cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt atgtggagta
    11281 atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg gatcccccgt
    11341 aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg gaacatttct
    11401 acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc tacctcatca
    11461 ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg acctgtaatt
    11521 gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc tggtgttggg
    11581 atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc tggatcgcct
    11641 tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg gaaaccatat
    11701 tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc atcatcgact
    11761 aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg tgacaataaa
    11821 atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa gattgcacca
    11881 tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc tggtgcatca
    11941 atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt gatcgtaatc
    12001 ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg atttgctagg
    12061 ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc atggtcaacg
    12121 tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc ttttgccata
    12181 atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca cctttttatt
    12241 atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg taagattcga
    12301 actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg tctgtcttat
    12361 acgcgaaaaa agtacctttt ataaataaaa aaatgt
//
LOCUS       DAZKNO010000079        12143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000079 DAZKNO010000000
VERSION     DAZKNO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.080"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..565
                     /locus_tag="ACG8MY_001619"
     CDS             <1..565
                     /locus_tag="ACG8MY_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0430810.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            966..1571
                     /gene="fic"
                     /locus_tag="ACG8MY_001620"
     CDS             966..1571
                     /gene="fic"
                     /locus_tag="ACG8MY_001620"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0430811.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1587..2159
                     /locus_tag="ACG8MY_001621"
     CDS             1587..2159
                     /locus_tag="ACG8MY_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0430812.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2411..2668)
                     /locus_tag="ACG8MY_001622"
     CDS             complement(2411..2668)
                     /locus_tag="ACG8MY_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430813.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2794..3609)
                     /locus_tag="ACG8MY_001623"
     CDS             complement(2794..3609)
                     /locus_tag="ACG8MY_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430814.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4126..5451
                     /locus_tag="ACG8MY_001624"
     CDS             4126..5451
                     /locus_tag="ACG8MY_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0430815.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5444..5794
                     /locus_tag="ACG8MY_001625"
     CDS             5444..5794
                     /locus_tag="ACG8MY_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430816.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5791..5961
                     /locus_tag="ACG8MY_001626"
     CDS             5791..5961
                     /locus_tag="ACG8MY_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430817.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6865..7140)
                     /gene="prgO"
                     /locus_tag="ACG8MY_001627"
     CDS             complement(6865..7140)
                     /gene="prgO"
                     /locus_tag="ACG8MY_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0430818.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7112..8065)
                     /locus_tag="ACG8MY_001628"
     CDS             complement(7112..8065)
                     /locus_tag="ACG8MY_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0430819.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8677..10170
                     /locus_tag="ACG8MY_001629"
     CDS             8677..10170
                     /locus_tag="ACG8MY_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0430820.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10305..10601
                     /locus_tag="ACG8MY_001630"
     CDS             10305..10601
                     /locus_tag="ACG8MY_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0430821.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10702..11553
                     /locus_tag="ACG8MY_001631"
     CDS             10702..11553
                     /locus_tag="ACG8MY_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430822.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11574..11786
                     /locus_tag="ACG8MY_001632"
     CDS             11574..11786
                     /locus_tag="ACG8MY_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430823.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4170 a         1864 c         2268 g         3841 t
ORIGIN      
        1 ttattttaag tctgaaaagg atacatcgtc tctaacgtta actttagagg aaggagttga
       61 acctgacaag gtgacctcta aagtgataaa ccaattgaaa gaaaaaggta gtttgaaaca
      121 tttgggggaa tatgaagtgt tagatacggc tatgttaacg aaaggaattg gtcaaatcct
      181 cagtacgatc acttatttta taacagctgt agcaggtata tctttattta tcgcaggggt
      241 aggtgtaatg aacatgatgt atatatctgt atctgaacga acaaaagaga tcggtataag
      301 aagagcccta ggagctactc gaaaatcgat tatgctgcag ttccttttag aaggtttgat
      361 tttaactctt tcaggagggt taattggcta tttattagga atggctcttg cttatagtgt
      421 tggttctttg ataaaggtaa acgtgtcact tgatctattt actattttac tagcggttgg
      481 ggtatcttcg gttattggac tggtcttttc tgtaatgccc gcttcagagg ctgcaaaaaa
      541 agatttaatt gatattctta gataaaatca gaatgacaag aatatttacg ttctagattc
      601 taaaataaaa cacaaagaga ccaaccaggc accctattgt ttctttgtag tatagagata
      661 ttctttggct tgtaagagat caatagagtt aggtgaccgg agttcgaaat aaaggaaatt
      721 tttgattgta taggagaatt ctttatccct aatatttaga attttgagta tgccatgttg
      781 atgttttaga ttctctataa atatagaggt gtatcaaagg tcaactagac tgtgcaaaat
      841 agagacactt gaaaaatcta tcgggggtca gaaacttaat agtttttgac acataaacaa
      901 aatcgcgata taatggggtt gagagtgttt cataaactta tgtcataaag ttaaggagat
      961 ttcgcatgat tttagagaat aaattgggat taaccaatca agttgagttg gctaaggttg
     1021 aagaaaaact cagtaaacaa aaagcgaaag aattgtacga ttgtggaaaa ataaatgaaa
     1081 tcgaagttgg tacatttaaa ggtttatctg aaattcatga atttctattt tcggatattt
     1141 acgattttgc cggaaaaatc cgatcggtca atattgcaaa aggaaatttt cgatttgcac
     1201 cagttatgta tttagaacat tcgttacagc atattgatca gatgccacaa acttcttttg
     1261 aagagattat caagaaatat gtggagatga atatcgctca cccatttcga gaaggaaatg
     1321 gccgaagtac tagaatttgg ctggatctca ttttaaagga agaattgcaa aaagttgtag
     1381 attggaactt aatcgataaa gcagactatc tttctgcaat ggagcgaagt cccataaacg
     1441 atttggaaat acgctatcta atttccaatg ctctgactga taaaattgac gatcgcgagt
     1501 tatatatgaa aggaatcgat gtcagctatt tctatgaagg gtattctgag tatacgatag
     1561 atgatttgta aaagtaggtg gaccaaatga cgaaatatgg atatgcacgt gtctcaaccg
     1621 cttctcaaag tacaaaagaa caaattcaac agttgcttga gaacggcgta gagaagaaga
     1681 atattttttc agaaaaattt acgggtacaa aacgggatcg gccaaaattc aatcagctgt
     1741 tggctggtct gaagcctgga gatgaaatag ttgttacgaa gcttgatcga tttgctcgta
     1801 acacgagaga agcattagag atcattgaac ctttactcga tgataacgta acgatcaagg
     1861 ttttgaatct aggaacgatt gaaaatacgt ccatggggcg aatggtgaca cggacgttgt
     1921 tgagtgtagc agaaatggaa cgtgatatga ttgtggaacg tacccaagaa ggaaaaatgt
     1981 ttgcaagaaa gaataatcct aatttcagag aaggacgtcc gaaagcaaca atcacaccga
     2041 agaagaggca cgcatatgaa ctgcttactt caggtaaatc gtataaggaa gttgaagcta
     2101 taaccggttt cagtcgcagt acgcttttta gaataaagaa gaaaattgaa gaaagttaaa
     2161 aaagaagtct cagcgaacca gacaggtaaa tacttaatgc gttctagata tgagtctatt
     2221 agcatgttgt gaaatataat atttttttat ttacttgatt tctgctcaaa aaatgtgtat
     2281 ggtaactgta aaaggtaaag taattaaagg tatgctagcc acgcttacta tattaggata
     2341 ccatcatata tttataatga acgatttgtt cgtctaccaa acaacacata tcgttcagcg
     2401 taagtcacgt ttaggaacgt ggctttttta gtgttattat cgataaataa tctattgact
     2461 ctattgattt tatctccata tccctacggt gttgattgtc tttagatctt tcatttaaga
     2521 accaaataac tatcataaaa atcagcactg ctgctaatat aataaacagc attcctattg
     2581 tgatattacc tattgatttt accataacta cgattaaagt aataatggca ttcaagtctc
     2641 ctttgttctc tatatataaa ttagccataa atcatcttcc tttcaaatta agatacttgt
     2701 ttgataccaa taaatagtaa gcgtggctag catacctttt tctgtaatag caagtgttct
     2761 aatagtccaa acttaatgat ggaccgatgc tagttatagt acagtaatgt tttctttctt
     2821 atctagattt ttagtaagct ctatcagcaa atctaacaaa tctgagtcct tttcagaaac
     2881 taaattaaat ctagtgttta caacaaagtt ttcatgttct atattgttta gattatttgc
     2941 ggaaaggagg gcttctagat cttgacattc aaatctatat gcatcgtcat aaatcttact
     3001 agaaggagtg cttaattcga aattacttat tttctcatct tggaccaaga taaatttata
     3061 gatagtatct ttttgtgttt ttcttttaaa aacacaacag agtgtgttat aaggaacgtt
     3121 ataattttca tcagcctcat tttttctcca ttcaaacaaa tgataaactg caaccaagtt
     3181 gccatcaatt tcatggacag tgctctcggt aacatccttt aaagaactat caagggctaa
     3241 tttatagtaa ctagagagag gaagtaaaat tgtcgattta ctgagatggt tatcgtctat
     3301 acgtgcaatc atacctaaat caactaaggt tttattccag gtgtttatag ttttagttgt
     3361 cactcctaga gactctgcta tggtattgac cgaatgccag gaatccccgc cttcattctt
     3421 tgccttaaaa cagtaataca gatataaatt aatagctttg ttattcggta aatatttttc
     3481 aagttctgtt ggtagaataa agtaagaatc cttaatgttt tttctgaact gtctccagtc
     3541 tctgtagttt tgtttataaa gttgtgaaac ggtttttcca cctttagaaa gtcctgctat
     3601 cttaaccaat cttattgctc ctttcttgaa tctgtcatga tcatacagta ttttttactt
     3661 gtagtccagc accttaatat attatcctat gtaattaatt tcgtactaga tagtaattat
     3721 tttttattta attctctagt cacttacaaa taagttatat aaactacgat taataaatta
     3781 ttacaaaaac tattaagaaa acttctacat acaactatta caaaactatt aagaaactga
     3841 atactcctat tttttgtttt taaggaaagt tttttctttt ttgaatttct tatttttttc
     3901 taatataact aagcaaaact ggataatacg acacttattt tcaaattttc acaagactaa
     3961 tagtaaatat ccatttaata aagtgataca atgtgttgat tttacaaatc aacagaagaa
     4021 gtagggaaat aacttaccta tgcttctttt tttgtacatt taatcgattc ttctttacaa
     4081 aaacgaacgt atgtttgtat aatgtggaag aatggagtgt tatttatgat ggattattca
     4141 aaagagccag taaatgatta ctttttgatc gatatgaaaa gcttttacgc aagtgtcgaa
     4201 tgtatagaaa ggaatttaga tccattaacg actgaattag tcgtcatgag tcgagcggat
     4261 aatacaggat cagggttgat tttagcttct tcaccagctg caaagaagaa gtacggtatt
     4321 tctaatgtca gtcgaccacg agatctaccg caaccttttc ctaaaacgtt acatgtcgtt
     4381 ccaccacgca tgaatctcta tattaagcga aatatgcagg taaacaatat tttcagaagg
     4441 tatgtagcag acgaagattt attgatttac tcgatcgatg aatcgatctt aaaagtgacc
     4501 agatcaatga atctttttac gactgaagga acaagaagtc aacggagaaa gaagctggct
     4561 caaatgatcc aggagaatat taaagaagag ctaggattgc ttgctacagt aggtgtcgga
     4621 gataatccct tattagctaa acttgcttta gacaatgaag caaaacataa tgatggattc
     4681 cttgctgaat ggacgtatca aaatgttcct gataaagtat ggaatattcc tgaaatgaca
     4741 gacctttggg gtattggatc tcgaatgaaa aaaagactca accaaattgg tatcatgagt
     4801 attaaagact tagctaattg gaatccatac actattaaga gccatttagg tgtgattggc
     4861 ttacagctgt attttcatgc gaacgggatc gatcgaacag atatagcgat cccaccagaa
     4921 ccgacaaagg aaaaatcata tggtaattca caagtgttac cacgagatta taccaggaga
     4981 aatgaaatcg aactggtggt aaaagaaatg gcggaacaag tagctattcg tatcaggcaa
     5041 cataactgta aaacaggttg tgtccattta tacattggca cgtcgatctt agaaacgaga
     5101 ccaggttttt cacaccagat gaagatccct ataacagata atacaaagga attgcagaat
     5161 tactgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggca cgttggaata
     5221 acctatagca agcttgtgta cactgattcg cttcagctgg atttatttag tgatccacaa
     5281 aagcaaattg acgaagaaaa tttagataaa attattgaca agattcggca aaaatatggt
     5341 tttacgtcga ttgtacatgc ttcttctatg ttagaaggcg ctcgtagtat tactagaagt
     5401 acattggttg gtggccatgc aggcggcgca ggagggctta aaaatgattg attataatga
     5461 tcgtggacgt ttaaagtggg caggttttta tttatctgat catactgaaa aaattgatgc
     5521 cgacaatgaa caacgagcca atcaaaattt ggcaaaagaa caaatgacga ctgaagaggt
     5581 tgcggaagta ttgaacgctg cagtcataaa aaatagatca gtatgtattc aaaaagaaga
     5641 acgaaacgct gaaggatact atccagaaga tattgttagc aagatcttag gtagtgatga
     5701 gctggggatc tacgttggaa aaaacaaagt tcactacgac gaaatacgtc atatcgaatt
     5761 ttaccaggat aataaatggg gtcgattaga atgaacatta tccagcaata tgagctaaag
     5821 tatataacct ttgatcagct atcagaagag atttggggtt atggccagcg tttgataaat
     5881 gaggttggtg ttgaacgctt ctctttctat gtagaagctg cagcaggata ccataaatac
     5941 gaatactata ttggctatta aagtgcaata aaattatatt tcttttatgt tttacatttt
     6001 gtatcaattc tgataaaatt acctataggt aattaaaaaa tataaaaaaa ctagttacaa
     6061 ccttaggacg ttgtaactag cttgaagcat actagagcta actaaaatag cactagaaag
     6121 caatgttaaa cccgtccaac aaacttgatt taacattgag accagcttat tcagctaaat
     6181 atagttgaat aagcttcttt tgttttaaaa aaacaaaagg tgtactacga ataacgcaag
     6241 taaaagaaat actattacaa ccagtaggct acaagtaagt attactaaac attgcgtagt
     6301 tagaaccttt gctctattgt ctaaagccac aaaattagag atcagcttta tgatgctaag
     6361 agcgagctgc atccatgcgg acataggttc ttttgacact attaattctc ctttttagga
     6421 taagggaaat tgctagtagt tttttcatta gcaccatccc ccttcatttg attgagtcta
     6481 gtgctgctgt gatagtttgc tgtttaatat gcttacattc ttattataac ataaatgaaa
     6541 caaacacatg gttagaggtc gtatcaatca aaatagtgtc tcacaaacga tttattactt
     6601 actcctgctg actaacggac tgttcttcag taactgtaac tatatgtttg ttaaaaccac
     6661 cattacctgt agataaaaac caatatattt actatactca ctatattaaa tatatttagt
     6721 atagtaaaaa ctagaaacaa taaaatttgt gaatgttgta tacccccaaa caatacacat
     6781 attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa atatatttac
     6841 tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata taatccgttc
     6901 ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt catacatagg
     6961 aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat ctacttgaac
     7021 tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa tatcatcttt
     7081 aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta ataatgccat
     7141 ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca gcatatttag
     7201 gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt ctttcttcta
     7261 attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa tcatttaatt
     7321 ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg cttttaaaaa
     7381 tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga gaggaacgtc
     7441 gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct tcaaaattcg
     7501 tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt gcgtaccgtt
     7561 ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga accacattat
     7621 ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat tcttctaaag
     7681 gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac aagtattcat
     7741 taagttcttc agatccagga gagccagcca taatatataa atgtggactt acttgagtaa
     7801 tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca aagctagtaa
     7861 tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa tcaattagca
     7921 acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg tctgtagtct
     7981 taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta tctgatgttt
     8041 taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa atatatttag
     8101 tatacttcat atacttaata tattcgatat atctaatata ctccaaattg ataagaattc
     8161 agactttata aggaatatag tggatatact aaatatattt gctatattca gtatatcaga
     8221 tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag agtgaaaaga
     8281 ggtaaactta attctataga aaccacagat aattcactat ttacaaagta gcaaaagcag
     8341 tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat tgattttagc
     8401 agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt ctcaagaata
     8461 tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc cgattgcctt
     8521 tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt gtacttaaat
     8581 taaatattct taaacactga agagagaaca catactatgg ctgagattaa agctaaactg
     8641 tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat ttattcagct
     8701 attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa acctgcaggt
     8761 catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca attaatgaat
     8821 gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga aaagaaacta
     8881 acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta tcgaacagtg
     8941 gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt tgatatcgat
     9001 tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt gggctacatg
     9061 ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact aaagacacca
     9121 ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa aatttcaaaa
     9181 acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg taaccacttc
     9241 ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa ctatcagtat
     9301 tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa aacaaaagaa
     9361 gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca agtagatgaa
     9421 ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg acgtcttggt
     9481 cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta tgcattggat
     9541 acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct atctgaagca
     9601 gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc aagcagagaa
     9661 ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa tgatctgttc
     9721 attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa atatagtcat
     9781 aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct tgctagtgag
     9841 ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc caagcgaaca
     9901 cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa ggtaacacga
     9961 ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag ggttattcaa
    10021 ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt tggactcaac
    10081 gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc tcttaaagaa
    10141 gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg ccttatacaa
    10201 tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt aggtggtata
    10261 cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg ttaaaaaatt
    10321 atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta tctgtggaag
    10381 tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt accagggaaa
    10441 aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct tctggggaga
    10501 ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt gagtataaaa
    10561 tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta tttacttaaa
    10621 aaattggttc attgagcaac ctatcgctgc taaagtagca aaggaaaacg ttaaaggaaa
    10681 gaatttaggg aagaaatcga aatgaaatcg gacgactgga ttgaacacac ctggcttcct
    10741 ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc aattatggcc attaggaaaa
    10801 ctttgtagtt acggggcacg acttattctt gatttaaccg tttactcgat tggagtattt
    10861 ggaacggctt attacttgat aaatgatccc aataggattg cagcatcaga catttttaca
    10921 gtgctaatga cgttgatttt tgtcatgcta ttgtctattt ttactcaatt agtcatgcaa
    10981 tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa gcctattcga tttaattgtt
    11041 gtaaatgcaa ttctagcgac tttaataaaa caaattgaac aatctggaag aacaacaaaa
    11101 ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat gtatttgttt
    11161 gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat taaacgttca
    11221 attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga gtacaatgtt
    11281 gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag tatccatgaa
    11341 tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa agcaatagat
    11401 cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata tggcatcgga
    11461 aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt ttggaacagt
    11521 agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg aaaatgattc
    11581 ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag aaggagcagt
    11641 tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt acgatgaaca
    11701 ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg ttgattcgaa
    11761 agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg atgaatggga
    11821 acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg taaagagcat
    11881 aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc tattacgttt
    11941 ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg atagtgaagg
    12001 atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc tatccttttt
    12061 aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa atcgtaagag
    12121 aaattgatcg aagatgtgac gaa
//
LOCUS       DAZKNO010000080        12056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000080 DAZKNO010000000
VERSION     DAZKNO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.081"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..536)
                     /gene="frr"
                     /locus_tag="ACG8MY_001633"
     CDS             complement(<1..536)
                     /gene="frr"
                     /locus_tag="ACG8MY_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0430824.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADK"
     gene            complement(539..1261)
                     /gene="pyrH"
                     /locus_tag="ACG8MY_001634"
     CDS             complement(539..1261)
                     /gene="pyrH"
                     /locus_tag="ACG8MY_001634"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0430825.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1397..2278)
                     /gene="tsf"
                     /locus_tag="ACG8MY_001635"
     CDS             complement(1397..2278)
                     /gene="tsf"
                     /locus_tag="ACG8MY_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0430826.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2377..3159)
                     /gene="rpsB"
                     /locus_tag="ACG8MY_001636"
     CDS             complement(2377..3159)
                     /gene="rpsB"
                     /locus_tag="ACG8MY_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0430827.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3518..3997
                     /locus_tag="ACG8MY_001637"
     CDS             3518..3997
                     /locus_tag="ACG8MY_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0430828.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4214..5305
                     /gene="phnW"
                     /locus_tag="ACG8MY_001638"
     CDS             4214..5305
                     /gene="phnW"
                     /locus_tag="ACG8MY_001638"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0430829.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5298..6083
                     /gene="phnX"
                     /locus_tag="ACG8MY_001639"
     CDS             5298..6083
                     /gene="phnX"
                     /locus_tag="ACG8MY_001639"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0430830.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6429..8120)
                     /gene="argS"
                     /locus_tag="ACG8MY_001640"
     CDS             complement(6429..8120)
                     /gene="argS"
                     /locus_tag="ACG8MY_001640"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0430831.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8542..9489)
                     /gene="arcC"
                     /locus_tag="ACG8MY_001641"
     CDS             complement(8542..9489)
                     /gene="arcC"
                     /locus_tag="ACG8MY_001641"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0430832.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9604..10623)
                     /gene="argF"
                     /locus_tag="ACG8MY_001642"
     CDS             complement(9604..10623)
                     /gene="argF"
                     /locus_tag="ACG8MY_001642"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0430833.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10714..11943)
                     /gene="arcA"
                     /locus_tag="ACG8MY_001643"
     CDS             complement(10714..11943)
                     /gene="arcA"
                     /locus_tag="ACG8MY_001643"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0430834.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3297 a         2519 c         2178 g         4062 t
ORIGIN      
        1 tctttatcag cagcgatctt atctacttct ttaatgctgt tatctgtcaa tgtttgtacg
       61 tctttttcta atccgcgcaa atcatcttcg gtaatatcac catttttttg ttgtttttta
      121 tattcatcga tcgcatcacg acggatgtta cgaatcgcga tttttgcatt ttctgcttct
      181 tttttcacat ctttagctaa ttcttttctg cgttcttctg tcagttgcgg gataactaag
      241 cgaatgacat ttccatcatt ggttggactg attccgatat cacttgccat aatagctttt
      301 tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca ttaaaacacg tgcttctgga
      361 atttggattg atgccatttg gttcaatggt gttggcgcac cgtagtagtt cacagtaata
      421 cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt gcccaagttc acgttgaaga
      481 ttttgtgctg cttttttcat tttttctttt gcttctgtca taattgcatc tgccattttt
      541 atttccccct tacagttgtt ccgatatttt cgcctaagat tgcacggcga atatttccgt
      601 gttcatttaa attgaagaca agcagtggaa tatcattatc catgcttagt gaacttgcag
      661 ttgaatccat gacttgtaat cctttggcaa taacttccag atgagtcagt tcttcaaatt
      721 ttactgcatt tgcatctact ttcggatcag cagaataaac accgtctaca ttgtttttag
      781 ccatcaggat aacatctgcg ccgatttctg ccgctcgtaa tgctgctgtt gtgtctgtcg
      841 agaagtacgg gttgccggta ccgcctgcaa agataacgat ccgtcctttt tctaaatgac
      901 gttctgcttt acggcgaata tatggctctg cgatttgacg catttcaatc gaagtttgca
      961 cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa agctaaggca ttcataactg
     1021 tagctaacat acccatgtaa tcagcttgag cacgttccat gcccatttgt gctccgattt
     1081 gtccgcgcca gatatttccg ccacccacaa cgattgccat ttcaacgccc agctcgtgaa
     1141 cttctttgat ttcttggatg atttctttga ttactggtgg tttgattcca aatcctgcat
     1201 ccccagctaa agcttctcca ctcaatttta agactacacg ctgatatttc ggtgtgacca
     1261 tgttcattcc tccaatattt tctattgcca ttttaccata cttagatgat ttcgacactc
     1321 taagactggt ttttcttgtt ttgcttaaaa aaagggagcg cacaaaccgt gcgttcccgt
     1381 ctgttattca acagaattat tttttcactt ggctcataac ttcttctaca aagttatctt
     1441 cgcgtttttc gataccttcg ccaacttcga aacgtacgaa tgttttaaca gttgcgcctt
     1501 ttgatgcaac gtatttttca acagtcatat caggatcttt aacgaatggt tggtccacca
     1561 aagcgatttc tgctttgaat ttcttcaagc gtccttctac cattttttca acgatattcg
     1621 ctggtttgcc ttcgttcaat gcttgttctg taagaacagt tttttcatgt tctaattcag
     1681 cttctggaat ttgtgattcg tttacataac gaggattgat agctgctacg tgcatagcta
     1741 cgtctcttgc aactgtttcg tcagttgttc catctagaac agtcaatacg gcaatacgtc
     1801 cgcccatatg caagtatcca ccaaatgcag cattgtcgtc tttttctaca acttcaaagc
     1861 gacggaagct gattttttct ccgataactt gagttgcttc gatcaagtca gattcaattg
     1921 tacctttatc tgttttgatt ttcattgcag cttccatgtc tgctggtttg ttttcagcaa
     1981 caagttctgc aatttctttt actaaatctt ggaacatttc gtttttagaa acaaagtctg
     2041 tttctgagtt tacttcaacg attgctgcag tgttaccttt cacagcaact gaagccaatc
     2101 cttcagctgc aatgcggtca tttttcttcg cagcctttgc cattcctttt tcacgtaaaa
     2161 gatcaactgc tttttccatg tcgccttcta cttcaactaa tgcttttttc gcatccatca
     2221 ttcctacgcc agtcatttcg cgtagttctt ttaccatttt agctgtaacg tctgccatgt
     2281 ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc ttcaaaggag agtgaggctt
     2341 gtcgcccagc ctttgaaaca gctccatcat tcaaaattat tctgctgaag aattgtcgcc
     2401 ttcaacaaca tctacgattt cttcgattga tgttgctgtg ttttcttctg tgaaatcttc
     2461 ttcaactact tgatcttcac cttggtttcc ttcgatgaaa gcatcagcca tttttgaagt
     2521 aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg attactacgt cgatttcatc
     2581 tggatcgcag tttgtatcaa ccatagctac gattgggata tttaatttgt gtgcttcttg
     2641 aacagcaata cgttctttac gtgggtcaac gatgtacatt acatctggga ttcttggcat
     2701 atcagcgata ccgcctaaga atttttcaag acgttcacgt tctttgttca aaccagcaac
     2761 ttctttttta ggtagaactt caaaagttcc atcttcttcc attgcattga tttgtttcaa
     2821 acggctgata cgtttttgga tagtatccca gtttgtcaat gttccgccta accaacgatg
     2881 gttaacgaag tattgtcctg cacgagttgc ttcttctttg attgcttctt gtgcttgttt
     2941 ttttgtacct acgaataatg ctacgccgcc ttcttcagca acgctcttca tgtaatcata
     3001 agctgcatct actaatttaa ctgttttttg caagtcaatg atgtagattc cgtttctttc
     3061 tgtgaagata tatttcttca tttttggatt ccagcgacga gtttggtgac caaagtgtac
     3121 gccggcttct agtaattgtt tcatagaaat tactgccatg tttgtttcct ccgatttggt
     3181 tttatttttc ctcttcttat cttctacctt gccggcaaac tttttcaagc acctaccgac
     3241 aatccaataa gaatgtggat ttgctgatgc ataaagcacc ctcagctatt atacagacaa
     3301 aagatccgta tcgcaagagc aaatatactt ttctgctcaa cattcaatag tttacctgaa
     3361 agataaaaag aattcaagaa aaacagtaga tttctctttg tttctttatt atcctttttc
     3421 cataaaataa ttttaggtat aaaagacgaa tattattggg caatccttct tttttttgtt
     3481 atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg cgcaaagctc aaagacattt
     3541 gctgattaaa caagtcattg aagaattccc gatccgtacg caagaagaac tattaaacaa
     3601 acttgggcat tatggcgtta ccgccacgca ggcaacgatt tctcgagata tccgtgatct
     3661 aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc ttcgtattgt ttcgcgaaaa
     3721 aggaactcct gagactagga ctgaggaaga ggatcggctg gttcaaatga ttgaagacat
     3781 tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc aataccttgc cagacaatgc
     3841 tcatttattt gcatctgttt tagatgagat caaacctccc tctattgtca gtacaattgc
     3901 tggattcgat acgatcatgg tcatctcgga aaatgaggag gacgcaaaac gagtagaaga
     3961 atttcttcac catccaactg aagtatcact tttttaatca gagcacgtat tcctaatatg
     4021 gaatgcgtgt ttttttattt tcaatatttt ccttattaaa taaaccttta cactctttac
     4081 aaaaatacat cattttttat tcaatttttt caacaccttg attgaatcac aataatcatc
     4141 atttatattg gatgttaaca agagcagctt cttgctcttt gtgtatttca aaaaagaaat
     4201 gaaaggaaat catatgtata aattattaac tcctggacca ttgacgactt ctgaaacagt
     4261 gaaaaatgct atgctgatcg atcattgtac ttgggatcaa gaatacaaag aaattacaca
     4321 atggataaga aaaaaattat tggatcttgc tcaagtatcg gaaggaatat acacaacggt
     4381 cttaatgcaa ggtagcggct cctttggggt agaatcggta ttaagttctg tgatcaggcc
     4441 agaagatact gtactgattt gttcaaacgg cgcatatgga caacgaatga tcaacatgtg
     4501 cgaatgtctg aaactgaact ataccgttta tgaggtatcg gaacatcaaa tcctccaagc
     4561 tcatgtgatt acacagctga ttgaagagaa tccctctatc tccgcactag caatggtcca
     4621 tagcgagact acttccggta ttttgaataa tttggaagag attgctgctg tcacacaaga
     4681 aaaagatctt ttgtttatag tggacgcgat gtcaagcttt ggtgggatac cgattcgtgt
     4741 agccgaacta gggattgatt ttttgatttc cagctcaaac aaatgtattc aaggtgttcc
     4801 aggcttttct tttgtacttg ctaataaagc ggaactagaa aagcgaaaaa atgtgagtcg
     4861 aagtttgtca cttgatctat atgaccagta ccgcgtgatg gaaaacgaga atggcaaatg
     4921 gcgtttcact tcccctaccc atacggtttt ggcttttcaa caagcgctga aggaactaga
     4981 ggcagaagga ggaatcgaaa ctcgatattc acgttatctc gccaataatc atgtactacg
     5041 acaaaaaatg acagagatgg ggttccagcc tttcattcaa gaatatcagg ggccattcat
     5101 cacctctttt ctttacccta aggactggcg atttgatttt aatcattttt atcagtattt
     5161 aaagcaaaac ggatttgtga tctatcccgg gaaaatcagc gatcatgaat gttttcgtat
     5221 cggaaatatt ggcgagatct atccggaaga tattttggaa ctgacagcaa ttatcaaaga
     5281 ctataaggag aacgttaatg attaaaactg taatttttga ttgggcagga acaactgtgg
     5341 actttggctg tatggctcct gtccacgctt ttagaaatgc ttttctagaa aaaggaatcc
     5401 aactgactga caaagaaatc cgcgaaccga tgggaaaatt aaaatgggat catatccaac
     5461 agcttctttc cctcccttct gtcaaaaaac aatggatagc tatttatgga caactacctc
     5521 aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca ttatctgttt aaagatttgg
     5581 tcaagcattc taccttgaaa cccgacacat tggaaacgat cgaacggttg agaaacgctg
     5641 ggatcaatat tggttccacg actggattta ctgctcctat gatgacaatc gtagcagaaa
     5701 ccgcaaaaaa agaagggtat tcaccagact tcatggcaac ttctgaagac gtcgaagggt
     5761 atggtcgacc gtttccttat atgatccata aaaacatgca gtacttcaaa aacaagtcgg
     5821 ttgatgaagt aatcaaagtc ggagatacgg tctcagatat ccaggaaggg aaaaatgccg
     5881 gagtgttgac agtaggtgta attgaaggta gttcgctaat ggggatttct tatgatgagt
     5941 accaaaaatt agagatgggg aaaaaagatc gtttaaaaca gtctgttgct cttagattcc
     6001 aacaagctgg cgctgatatt tgtgtaaaca atttaaaaga gttaacagaa tacattttgt
     6061 acgctgacaa gaatccagaa tgacttttat acaaaatcag aggtcgtgaa aaagctgtac
     6121 tgcatcaagt aataagaaca gccaaacaaa aatagctcct catatttttc gtttggttag
     6181 gcttattacc gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa agggagctgt
     6241 gacacgactt ttgtcacagt ccccttattt acaaacaaac agtgtttaag agacaacctc
     6301 tccactaaac ggtgttcatt cagctgactc tcggtatttg ctcaaaaaac gataaaattt
     6361 gaaaagctat tttctctttt gtttctctaa tactcggatc agaacgctga tttcacattc
     6421 tctttttctt acatcttatc tggtgcatga agtcctagca aacgaaggtc ttctttcaat
     6481 aatacagtca ctgcataaac tagagctaag cgtgcttctt tttgttcatc atcagctaaa
     6541 atcttcgtat gagcatagta tttgttgaat gcttgtgcta atttgattgc atgtttcgca
     6601 atcactgacg gttcgtattt ttccccagca gacaagaccg tttcagggta tttttgaact
     6661 aatttaacca cttcccagct ggtatcgtca ttcaatgcat agtctgctgt ttctgaaggg
     6721 acaaatcctg ctttttccaa caggctgact gcacgagcat gtgtatattg cacataaggt
     6781 ccagtttctc cttcaaatcg aacaacttct tccaggttaa agtcaaatgt attcagtcga
     6841 tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa cttgtttagc tacagcatct
     6901 ttgttttcca aatcagggtt tttctcgctg atttgttctt tagcagattg gattgcttca
     6961 tttaatactt cttctaataa gacgatcttt cctttacgag ttgataattt tttaccacct
     7021 tgcgtgatca aaccaaatgg aatatggcgc atatcgtcag accagtcaaa gcccatttcc
     7081 ttcaagacag ctttcaactg tttaaaatga tagctttgtt cattccctac tacatataga
     7141 gattgtttga agtcgtaagt acgtttacgg tacaatgcag cagctaagtc acgtgtaatg
     7201 tatagtgtcg caccgtctga cttcttaatc aacgctggat tcaggtcata agcagatagg
     7261 tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt tttcttctaa aagtttgacg
     7321 atttcatcca tcttgtcatt gtagaaagct tcaccgttca acgaatcgaa acgtacttcc
     7381 agcagatcat agattttatt gaattctttc atcgattcat cacggaacca ttgccataat
     7441 tgaatagcct cttcatcgcc ttcttccaga cgtttgaacc aagcacgagc ttcttcattt
     7501 aattctggtt ctgtttcagc tacttcatgg aattggacat ataaacgaag caattcattg
     7561 attggttctg ctttaacagc ttcttctgta ccccattttt tgtaagcgac gatcagtttt
     7621 ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga ttggctgata accgattttt
     7681 tccataatga aaccgatcga attaccgata actgtcgaac gcaaatgtcc cattgagatt
     7741 ggtttggcaa tattaggaga agacatgtcg attggtactg ttccttgatt cccgatattg
     7801 ctatccccat aatgttcttt ttctttcact actgtttgca aaactttttt gctgataagt
     7861 tctttattca tgaaaaagtt caagtaaggt ccgacaactt cgattttttc aaagttggca
     7921 gaatcgattt tttctgctaa atctgcagcg atttgctgtg gcgcttttcg gtaaactttt
     7981 gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag ctgattttgg attttctagt
     8041 aattgttcga cttgttccag tgttaaatca tctttgacag cgtcatagac agcttttgcg
     8101 acaagatctt tattattcat ctttattcct cctaaagtgc tcccttgatt gttaaacaaa
     8161 aaaaatctct agcgacggca tacttagtat gccatcacta gagacgagat ttcccgcggt
     8221 accactccaa ttgtcctaaa aaaggaccag ctccatctga tatcggtaga cgaccgtctg
     8281 tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc ttcattcggc ttccaccact
     8341 tccgaattcg ctagccaaga atgatacaag tactctcttg ttcaacaaat cttagtctta
     8401 ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg ctagtcgttt ctgataaaaa
     8461 aacaatccag atggcctaaa ggagaagaaa taccacctgg atagctataa atcatttcgt
     8521 aaatacgaaa gacttaacaa attaatcagc tacgataatc gttccttctt cagaagctaa
     8581 aagattctcg atattttcta aagaagtgat gattgcctta gcgtttggtt ttgcttcaac
     8641 aaatgcaata gctgcctcta ctttcggaag catgcttcct ggagcaaatt gcttttcatc
     8701 aatgtattgt ttcatttctg aaacagtaac agtttctaat ttcttttgat ctggtttttg
     8761 gtaattgaca tacacattgt ctactcctgt caatacgatc agcaaatccg catcgatgat
     8821 ttcggctaat ttttctgaag caaaatcttt atcgattacg gcttctcgtc cttctaatcc
     8881 agtagctgtt tctacaacag gaatcccacc gccaccaaca gagactgtga taactccttg
     8941 atcgaccagc gtttcgatga cacgtgcttc tttaatcgag atgggtttag gggaagcgac
     9001 tacttttctc catccacgtc cagcatcttc tttgaatgtc acagtggagt cagcattcat
     9061 ttgttctttg gcttcttctt ccgtgtagaa tggaccgact ggtttacttg gatttttgaa
     9121 tgaaggatca ttttcatcca caataacttg agtgactaaa gaaaccacat ctttatcgat
     9181 ccctttttcc ttcagtactt cgcccatggc attttgtaac cagtaaccga tagatccttc
     9241 tgtcattgca acacatgtat ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg
     9301 ctgctgaatc aataggttac cgacttgcgg accatttccg tgggagatga tcaattcatc
     9361 tccttgttcg ataaatttca ctaaatactt tgctgtttcc attaacgctt cttgttgggc
     9421 ttttgcactt gcatccgtag acaagattgc attaccccct aaagcgacaa ctacttttcg
     9481 tttggccatt tcattcgtcc tttcttcagt tctaccgaat tttctagata acaaaacgag
     9541 ccagcgtttt gtgattttcc ctcaatgctg actcgttttt tcgtgagatg aatgtggtta
     9601 tgcttaaacg cgagggataa acaagttacc taatgttgct gccatgatcg ctttgattga
     9661 gtgcatacga ttttctgctt ggtcaaattg acgagcatat ttgctgcgga agacttcatc
     9721 tgttacttcc atttcagtga tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt
     9781 tgtatcatgg aaagctggca agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt
     9841 ttctaccatt tccatattga tttggtaagg tttcaacaat ttgatacgtt cttcgaattt
     9901 atcttcttca cccattgata cccatacatc agaataaagg acgtctgctc cgtctacacc
     9961 tttatcaaca tcgtcggtaa tcatcaattg tgcaccggat ttttcagcaa agccttctgc
    10021 cattttgacg atttcttctt caggttgtaa ttcttttggt gcaacgatac gcatattcgc
    10081 tcctaaaatt gcacctgtga ctaataaaga gttagccata ttgtttcgac catctccgca
    10141 gtaagcaaca gtgatacctt ctactgttcc aaagttttct tggatagtta aaaagtcagc
    10201 gatcatttgt gttggatgcc attcatcagt caagccattc caaactggaa caccagagaa
    10261 ttctgctagt tcctctacca ttttttgact gaatccacgg aattcgattc catcaaacat
    10321 acgacctagt actttagctg tatcttctgt ggattctttt ttacctaatt ggatatcatt
    10381 tgcacctaga tattcaggat gtgcacctag atcgatcgct gcagtagtaa atgcagaacg
    10441 agtacgtgta gatgtttttt caaataaaag cgcaatattt tttccttcta gatagtgatg
    10501 tgggatacca cgttttttca agtctttcaa gtgttctgaa aaatcgatca aatattgtaa
    10561 ttcttctttt gtaaagtctt tttctgctaa taagcttctt ccttggaata ctgattcttt
    10621 catattaaac attctccttt agattgtttt tatttaagat taaagaggaa atcccgacgg
    10681 cagctgacag taagaccgcc gtcaggaaac aaattacttc aagtcttcac ggacaagtgg
    10741 ttggctcatg cagcgtggac caccacggcc tcgtgacaat tcacttgaat tgatttctaa
    10801 cactttgatg ccatagctac gaagcaattc gttcgataca taatttcggt tataagtgac
    10861 tacaacacct ggagcgattg ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc
    10921 aacgatctca tctccattac cagttgggat cagtacaaga tcatctaatc caagagcagc
    10981 tttcatcgtt tcgtgtaagt tatctgaatg agtaatcttg atatcatcac catctggttc
    11041 gattgtaaag acatccatct taccgtcttt actttgaatt gctggatgga tagtgaattt
    11101 atcgtgatct accattgtaa atacggtatc caaatgcatc attgcacggt tgtttggaat
    11161 tttgattgct aacacttttt caaatcctga attttttgcg aataggtggc gtgctaattt
    11221 ttctaaagct ttcgcattag ttcgttgaga aatcccaaca gctactactt tatcgcttaa
    11281 gatcaattcg tctccgcctt cgatatgatc tggattttca cgatccaacc atacttcgat
    11341 tcctttattt gcaaatcttg gatgatgttt caaaatatat tctgtaaaca tcgattcacg
    11401 acgacgtgct tcaaaagtca tcttattgac tgtcatccca tttcccattg atgcggaagg
    11461 gtcgcgtgta aagtacaagt ttggcattgg gtccatgtaa aatggatagt catcgtctgc
    11521 tgacagatcg tatagactgc tagaacgtac ttcaatatct tttgtacgaa ccccagccat
    11581 gattttatct accatttctt gcgtttccat tgatagaagg aactcatgaa gaccctctct
    11641 tacagcattc gatgcaatat gagattcatc caacatttta ttcaagaatt gttctttgac
    11701 attgcctgcg tcaatcgctt ctgccgctaa tttttccaag taaagagtct cgacgccttc
    11761 attttgcaat gtctttgcaa agttgtcatg ttcttcttga gcaatcggaa gatatggaat
    11821 atcatcgaac aacagacgat ccataatatc tggtgtcaaa ttttccactt cttgtccagg
    11881 ccttttcagt aaaactgtct ttaatttccc tatttcagag aaaacgtgaa taggtttatc
    11941 catgttgtgt tgcctccttc tttttgatta catgtatagg atatcctcct tttgtaatca
    12001 ctttcataat tttgtctcat tcaaaatgag acattcctca cattaatatt cgataa
//
LOCUS       DAZKNO010000081        11977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000081 DAZKNO010000000
VERSION     DAZKNO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.082"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="ACG8MY_001644"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="ACG8MY_001644"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0430835.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /gene="rpoZ"
                     /locus_tag="ACG8MY_001645"
     CDS             951..1256
                     /gene="rpoZ"
                     /locus_tag="ACG8MY_001645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0430836.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="ACG8MY_001646"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="ACG8MY_001646"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0430837.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="ACG8MY_001647"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="ACG8MY_001647"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0430838.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /gene="fmt"
                     /locus_tag="ACG8MY_001648"
     CDS             4416..5354
                     /gene="fmt"
                     /locus_tag="ACG8MY_001648"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0430839.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="ACG8MY_001649"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="ACG8MY_001649"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0430840.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="ACG8MY_001650"
     CDS             6725..7465
                     /locus_tag="ACG8MY_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0430841.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="ACG8MY_001651"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="ACG8MY_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0430842.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="ACG8MY_001652"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="ACG8MY_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0430843.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /gene="rpe"
                     /locus_tag="ACG8MY_001653"
     CDS             10608..11258
                     /gene="rpe"
                     /locus_tag="ACG8MY_001653"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0430844.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="ACG8MY_001654"
     CDS             11259..11897
                     /locus_tag="ACG8MY_001654"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0430845.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
BASE COUNT         4004 a         2237 c         2645 g         3091 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagagga
//
LOCUS       DAZKNO010000082        11858 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000082 DAZKNO010000000
VERSION     DAZKNO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.083"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG8MY_001655"
     CDS             complement(<1..272)
                     /locus_tag="ACG8MY_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0430846.1"
                     /translation="MDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLKLLIHT
                     ACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITV"
     gene            573..2453
                     /locus_tag="ACG8MY_001656"
     CDS             573..2453
                     /locus_tag="ACG8MY_001656"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0430847.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2532..2759
                     /locus_tag="ACG8MY_001657"
     CDS             2532..2759
                     /locus_tag="ACG8MY_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0430848.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2764..3306
                     /locus_tag="ACG8MY_001658"
     CDS             2764..3306
                     /locus_tag="ACG8MY_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0430849.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3557..4234
                     /locus_tag="ACG8MY_001659"
     CDS             3557..4234
                     /locus_tag="ACG8MY_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0430850.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            4576..5796
                     /gene="sstT"
                     /locus_tag="ACG8MY_001660"
     CDS             4576..5796
                     /gene="sstT"
                     /locus_tag="ACG8MY_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0430851.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            5943..6578
                     /locus_tag="ACG8MY_001661"
     CDS             5943..6578
                     /locus_tag="ACG8MY_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0430852.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6769..7512
                     /gene="truA"
                     /locus_tag="ACG8MY_001662"
     CDS             6769..7512
                     /gene="truA"
                     /locus_tag="ACG8MY_001662"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0430853.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSLYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            7746..8579
                     /gene="msrA"
                     /locus_tag="ACG8MY_001663"
     CDS             7746..8579
                     /gene="msrA"
                     /locus_tag="ACG8MY_001663"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0430854.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            8660..9653
                     /locus_tag="ACG8MY_001664"
                     /pseudo
     CDS             8660..9653
                     /locus_tag="ACG8MY_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            9942..10640
                     /locus_tag="ACG8MY_001665"
     CDS             9942..10640
                     /locus_tag="ACG8MY_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0430855.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            10790..11245
                     /locus_tag="ACG8MY_001666"
                     /pseudo
     CDS             10790..11245
                     /locus_tag="ACG8MY_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            11326..11580
                     /locus_tag="ACG8MY_001667"
     CDS             11326..11580
                     /locus_tag="ACG8MY_001667"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0430856.1"
                     /translation="MTRYEENFKQMIIELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            11721..>11858
                     /locus_tag="ACG8MY_001668"
     CDS             11721..>11858
                     /locus_tag="ACG8MY_001668"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0430857.1"
                     /translation="MKKKISTIFFTNKQRYGATKIHQVLLKEGISVSLKHVQKLMKQL
                     NL"
BASE COUNT         3929 a         1914 c         2466 g         3549 t
ORIGIN      
        1 acagtgatcc caaattctgt ttcaatattt ttgaaggcct tttttacatg aaaaattgtt
       61 tcatgagaag gtcttgtgtc atcttctgat ataattttaa gttcatgacc gtttatcaat
      121 gtacgctcaa ttagacacgc tgtatgaatc aaaagtttta aaagaatgga atgagtgaat
      181 tttttgtgga aagtacattc caattcttgg ataaaatcca ttaagctttt ttggatctta
      241 ttcccagcta ataagtagag atatttgtcc actgcttcaa ctcccatatc aaaaaacgat
      301 atttcttgtg aatgagtggc tgctgccctt ttttgattgt tgttcttcag tcgtaagatg
      361 acaaagcatc ttatctttga ataaaaaaat agaactacct aaggaagcgt ttcggcgctt
      421 cctttttcta tatgatcgtt taatgactgt aggaattcag tataccaaaa gaaaaccgcc
      481 tttttgaata aacttgatag tggtcaattt tttatgatgg ataagtatct atacttagtc
      541 tcgtaagaat gaaaaggaaa agagggagaa agatgaaatt acaaaagtgg atccaagagc
      601 atcgaaatca tatcaccgcc ttatcagctg tgttgattat ttttggtttt atcgggaaat
      661 ttactggatt tgtcttgttc tataacattg ctctagccat agcttctatt attgcggtca
      721 tacctattat aatccatgcg tatcaggcag taaaagtcaa agttatctca attgatttac
      781 tcgttactat tgctgtgctt ggtgccttta tgatcggaga atacaaggag tcagctatcg
      841 ttacattctt gtttttgttt ggtagttatc tggaacaaaa aacattgacc aaaacgcggg
      901 aatcaatcaa ggctttgacc gagatggccc catcgactgc cagcgttgtc caagaagatg
      961 gaacgaccga agaagtggaa atcgatgacg tggaagaagg cgatatcgtc ttagtaaaaa
     1021 ctggtggagc tattcctgtg gacggacgag tcattgacgg gaaagggtat gcggatgaag
     1081 ccgcagtgac gggtgaatca cgacaagtcg tcaaaatcag tggagataac gtattttctg
     1141 gaacgatttt agcagatggc tacgtgacga tcgaagcaac taaggtcgga gatgatacga
     1201 cctttgccaa aattatagaa ttagtcgaag aagcacaaga taccaaatca catgcggaaa
     1261 aattcattga tcaattttct cagtattata ctccaaccgt tttacttatt gcgttgcttg
     1321 ttggtctgat cagtcgtgat tttcgtttag ccatcactgt tttggttctt ggttgtcctg
     1381 gcgctttagt cattggtgcg cctgtttcga atgtagcagg aatcggtaat ggagcgaaac
     1441 ggggcatctt agtcaaaggc ggtgaagcaa tggatacttt ttccaaaatc gatacttttg
     1501 tctttgataa aaccggtaca ttgaccaaag gaactactgc tgtatccact atcaagaact
     1561 atacagagga tgaaggggac agcttagctt tagtggctcg attagaaacg ttatccgatc
     1621 atcctctggg gcgcgccgtg atccattatg ctcaggagaa agacattgat tttcaacaat
     1681 tagcagtcaa agataatcag acgatcaagg gacaaggaat cacagctgag attgatggtc
     1741 atacagtttg tgcgggcaac accaaattga ttgccgctca caaactttct ctcacaccaa
     1801 agcaagtaca agatttacat caattacaac aaagaggcag ctcagtcatt attgtagcaa
     1861 tcgacaaaaa aatcactcaa atcattggtg tgtcagatgt gattcgacca gaagtcgctg
     1921 aacaattagc tatgttgaaa caagcaggag caaaacattt agtgatgctg acaggtgata
     1981 atcagatgac tgctgattat gtagctgaca tgttaggcat tgatgaagtc catgctgaat
     2041 tattgccaga tgaaaaggtt caatttgtga aaaaatacca agaacaagga ttgcacgttg
     2101 ctttcattgg agatggaatc aatgattctc cctctttggc tgcggctgat atcgggattg
     2161 ctatgggatc aggaacggat gtcgctatcg aaacgagtga cgttgtcttg atgcaatcta
     2221 gttttggatc gttggttcat gcttaccgtt tggctaagaa aactgttttg aatacccgtg
     2281 aaaatgtagt gattgccatt ggtgtcgtct tatttttact catcggctta tttgccgggt
     2341 ttatttacat ggctagtggg atgttcgttc acgaagctag tatcttgatc gttatcttta
     2401 acgcgatgcg tttattgtat tttggacacg aaagcaaaga agaaaaatcg taaaacttga
     2461 taagaatcaa tttttttatg gaagctgtaa tctagaataa gactatcaag aaaaaggaag
     2521 gaagaactac gatgaaaaaa atgattatgc aattagatac actagcttgt ccaagctgta
     2581 tgcaaaaaat agaacatgcg gtttctcaac agcctggggt aagcaatacg aaagttttgt
     2641 ttaatgcgag caaaatcaaa gcaaactttg atccagatga agtccaccct gaacaattag
     2701 ctgcagttgt ggaaaaatta ggttatgaag tccaatcaac aaaagtaaaa gaggcgtaaa
     2761 tcagtgaata tagtggaaga aaaatttgct caagaaacaa aacaagcaga tatcgatcat
     2821 cataaaccaa ctgctggcgc catggtgggg catgtattgg caaattttaa attagaagaa
     2881 atgaaattga tccaagcaca attgtatgtt ttgggtacat cttcgcaaga attaagtacc
     2941 cttttttcag aatatgctga tcaagaagcg agatggttca ataaaatctc tcatgagtta
     3001 gtgattgaaa atgaaatcgt tccgtctact ttagaagaag tcatgcgtta tggaaaaatt
     3061 tacgaacatg gtcaaaacaa atatttacca gcctcaacaa tgttggaaaa ctttgcaaaa
     3121 gattttgatt tccaaaatgt ctttatcaca cgggcaatca aattagctga aaaagaagaa
     3181 aaatatggct tgaaacaatt gcttgttgaa cttttaagtt tcaataaaga aatcatctac
     3241 caagtacaaa gactccaagg aaaaactgtc cgacaagatt tggatgagga agacgatgat
     3301 gactaaaagt ctatgtttct tcaaatacaa atataaagcg tgaggtaaat tggaaaatcg
     3361 cgaagattaa aataaaagaa gcacccgctg atgtattaca gacaacatca gcgggtgctt
     3421 cttagttttt aaaaggttta aaagatggat gagaaaatac aaaaaatcaa taaaatcaca
     3481 ttgcttattt gatagaataa agaagtggga gtaatcgaaa ggagtttttt aatttaaaaa
     3541 aaggatttta gtaacaattg gtctagcttt tttgttagct ggttgtacca acgggaatga
     3601 atcgaaaaaa gaatcaacaa aagaaacagc gaaagcaaca attgcaacta ctacaacaac
     3661 tagcacagag agcaagccaa ctgcagcaag tacggaaaca gcagatacct ctacttcgtc
     3721 agagtcttcc aaaacgactg aaacaactca aacatctact tcaagttctt ctgtagaggc
     3781 ttcatcttca gaaagcagcg aacaagtcga acaaacactg tggaatgcag acaaatccgc
     3841 acaattagag gcatttatgg ctacatgggg ccaaacaatg ggacaacaat acaaaagcta
     3901 tacgaatcaa atgagtgtgg acttatatgg tcttaaagtc cctcaagtta ttttgaacgg
     3961 ggagtggaag atggctattg gtggagtgcc tgtttctgca gaatggtcag aaagtgggac
     4021 aggtcaagca gactatcaaa tcacagccgt ctattccgat gcagagacag aaccatatct
     4081 gaaaaagcac gtctatctat tcgggtttca gcaaaatcag ccaaaagtat tggtgacaca
     4141 acaaaatcag gggaatcccg ataattatct ttactttaac gagacagcga ataatgaatt
     4201 gaaaaatggc tttaatcaaa tcgtttatgg gtaagtaaac cagtgagagt gagagaaaga
     4261 agagactcag tggaaaatat taattgattg ctatgtctaa gaattaagat gaaggatgaa
     4321 atttctagtt tcgtccttca tcttagttac gttttacttg gataactata ataataacga
     4381 taaatagttt tctctcgact tctttagaga ttgaataaac cctccaatac taaaattgaa
     4441 tgttgcaaga gcatgaattg ccaattgtat tattagaatt tttagaaaat tgcaccttta
     4501 gtttctttag ataaaatgat acaatgattg ttaaaattgt gaaagggtgg tcatttgtta
     4561 gagaagtttt tggatatgtc gttagttcaa cgtattgttg ttggtatttt agtcggaata
     4621 ttgtttggta tctttttccc cacgtggaca tttatttcaa tattggggac gttgtttgtc
     4681 agttgcttga aggcaatcgc acctattctg gtttttttct tgacgatggc gtcaattgcc
     4741 aagcataaaa ttgggaataa gacattcgtg aagcctattt tgatcttata tttagtggga
     4801 acattgcttt ctgcacttgt agcagtcatt gtaagttcaa tttttactgt tcctattact
     4861 ttacaagaaa cagtatctga aaaagcaccg caaagtttgg aaagtgtttt atctactatg
     4921 ttgacgaatg tcacacaaaa tcctgttcaa tctcttattg atgcgaatta ccttggcgtt
     4981 ttattctggg ctgtattgtt gggactagct tttcgagcac gttcagaacc gacaaaagag
     5041 ttggtggatc agatatctat agcgttgtca caagtcgtac aagtgattat tgcatttgct
     5101 ccgataggga ttttaggatt ggtttatcaa tcaattgcta cgactggaat cgctggattg
     5161 gcagagtatc tgcagctttt acttgttttg atcggtacga tgctgtttgt cgcattagtt
     5221 gtgtacccat tcctcacgtt tttgtttatt aaagaaaatc cctatccatt gattttcttt
     5281 tgtttgaaag aaagcgcact tcccgctttc tttacccgaa gttctgccgc aaatatcccg
     5341 ataaacatga tgttggctga aagattgaaa ttgacaaaag agtcttattc catatcgatc
     5401 ccgttaggtg ctacgatcaa tatgggcggt gccgcgatta ccatttcctt gatgacgttg
     5461 actgctgtac attctttagg gattgaagca ccatttgttt tgaaagtcct tttatgtatt
     5521 ttgtctgctt tagctgcttg tggagcttca ggtgttgcgg gcggttcact gttattgatt
     5581 ccattagcat gtagtttgtt tggtatctct aatgatgtcg cgatgcaaat cgttggtatt
     5641 ggatttatta tcggtgttat ccaggattca atggaaactg ccttaaattc gtcaagtgat
     5701 ctgttgttta ctgcaatagg agagctaggc gcgcgtaaaa gaagtgggga aaaaatcaaa
     5761 ttgaaagatt gtttagatgg attaagtgaa cgttgaacgt tagatattta gtatttttaa
     5821 aatgattcga gtttttaatg agttatatag atgttcgtgt aaatagaggt ttctgttaaa
     5881 tcaagataaa gtcgtttata ataacaatta aaagcatagg taattgttat tggaggcgta
     5941 tcatggattt tttacgtaaa tttggttttt tctttttatt attaggcgtg ctgagtgatt
     6001 ttttgactcc gtacgtatta ggattgtatt atcctgaatt agatcaaatg aagagcgtga
     6061 tcagtgtatt tggagaggtt tccagtcctg taagaaaagc gtttcttatt tggtctgtgg
     6121 tttcagggat ctttttcatc ctgtcattgc ctgcattgta tgctgcattt gtaagtacat
     6181 ctcgtgtgtt agctacccta gttacagcag ccatcgcgtt ctatggtata ggtgattgta
     6241 tctttacagg tttgttcagt atcaataccc atgaatcttc gtggaatgtc tcgacatggg
     6301 tccataatat cggatcaggt ttaggttaca gtggtttttt attgtttcca gttcttcttt
     6361 tcttgatcta tcgaaaaaat gggcaaatgg acgtaagcca cttatacctt ggactgtctg
     6421 tgatcagttt gctttttgcg gcgatttatg gactggcaag aattccttct ataaattctt
     6481 ggccattact gaataaaata ggtttttgcc aaagaatcag ttttttcttt aattatgtgc
     6541 ccattgcctg gttgagtatc ttgcatctca aacagtaaaa cttgagccgt caaattgaac
     6601 agtcagcgga tacttcatcc ggtggctgtt ttttgttttt ctgaaatata tatttccaca
     6661 gttttcttgt atagcttctg tcttagagaa gcaaggagct atattttgtg ctacaatctt
     6721 aaaaaggtag agagaagacc aaatggaaga cagaggagcc actaaaatat gagaaacatc
     6781 aagttgacaa tcgaatatga cggaaaaaga tattcaggat ggcaaagact tggagatgac
     6841 gataaaacca ttcaaggaaa aatcgaaaaa gtattgcatc agatgacaaa tgaagagata
     6901 gaaattattg gatcaggaag aacagatgct ggcacccatg cgcgaggaca agtagcaaat
     6961 tttaaaacaa acacagaaat tggactggca gaaatgatcg actttatgaa tcgttacctt
     7021 ccgcgggata ttgtcatcaa aagaatcgaa gaaatgcctg agcgcttcca ttcaagatat
     7081 aatgcggtgg gcaaaaaata cagctattat gtttggaaca acgtgattcc ttcagcattt
     7141 gagcgaaacc acagcttata tttccctcaa gagttggata tggacaagct gaatgcagct
     7201 tgcgaaaaat tagtgggtac acatgatttc attggttttt ctgcattgaa aaaatcaaaa
     7261 aaatccacag tgcgtacgat tgatgaatta tctatcgaac gaaagggaga aatgcttcat
     7321 tttacttttg tcggagacgg attcttatat aaaatggtgc ggattatcat ggggacatta
     7381 ctagaaatcg gcacaggtgc catgccgata gaaacaatcg atgaaatatt tgaaagcaaa
     7441 gtgcggcaac atgcaggaga aaccgtccct gcacaaggtc tgtttttgga tgaagtttat
     7501 tatcgctcat aaaaaagaat catctttact cgacttctga gaattggaga acatcccttt
     7561 tttcagaggt cgttttttag ttaaaccaaa ccatagagta tgctttttaa gataaagatc
     7621 atcttatatc atctgaagct gcttatcttc tttgaataaa tacgattcaa tcgattattt
     7681 cctcacttta tgataaatta cagctaaagt cactagcttg tcttcttcgg aaaggaggag
     7741 aatctatgga tgaggaacaa atattcattc aaacacttta taatctgatt ttgaatccga
     7801 atacacgaga ttgggaacga aaagtattga ttcagacaaa gaatgacaca agagaaaata
     7861 tatccgttaa agaacagtta tcaaaactag aagtaacact acgtccatta gctatccgaa
     7921 tgaatctgac gcctgatgta atggactttt atttattgat tacggaagga tttgataagg
     7981 agcaaaaata tgacttttcc aagcatgcca tgcaagatgc tgactatcaa gaacgtgcag
     8041 tatttgcagg gggatgtttc tggtgtatgg tcgagccgtt tgaatcgaaa aaagggatcc
     8101 tatctgtttt gtcaggatac acgggcggac atgttgaaaa accgaattat gatcaagtaa
     8161 gtggtggtta cacaggtcat gtggaagctg tggagatcat ttatgacaca agagaaataa
     8221 gttatagcga gttgctgaca atctattggc aaattactga tcccacagat acatttggac
     8281 aatttcaaga tagagggaag caatatcgtc cagtgatttt ttatcaagac gaacgacaaa
     8341 aagaattagc ggaacaatca aaacagaaac tagacagttc aggaactttc caccaaccaa
     8401 tcgtcacaaa aatcgaacca gccggtacgt tttggccagc agaaaactat catcagcaat
     8461 tttacaaaaa acaaccgaaa cgctataaaa aaatccagca agcacgtaat caatttctga
     8521 tctatcaacg agtaaaaaac aaatggcaaa aaaatatacg aaaaaatcat tttgattaac
     8581 tccttttctt taaaatgtag taaactaaaa atagaaaaca agaaagcgtt ttaaaaagat
     8641 ttgatgagga ggagaaaaat tgtatattgc agatagtgca cgttatgaaa aaatggaata
     8701 tcgtaaatcc ggtcattcag gtttaaaatt accgatttta tctctcctct cggattatgg
     8761 caaaattttg gtgattatga tcctatccat aatcaaagag agattttaag aggcgcattc
     8821 gacatgggaa ttacccattt cgaccttgcg aataattatg gaggccctgc tggagctgca
     8881 gagaaaaact ttgggcgtat ttttagagaa gattttcaag cctaccgtga tgaactgatt
     8941 atttctagca aagcaggtta tcatatgtgg gaagggccat atggggaatg gggctctcgt
     9001 aaatccatca tatcaagttg tgaccaaagt ttccaaagaa tggggctaga ttatgttgac
     9061 atcttctatc atcatcgccc agaccctgat acacctttgg aagaaacagc agaagcgttg
     9121 atgcaacttg taagacaagg aaaagcatta tatattggta tatccaacta taatggagaa
     9181 gatacaaaaa aatgactgaa attttaaaaa gaaaggaagc accgttcatt attcatcaga
     9241 tgcgctacaa tatgttttct cgtgcactac tagaagacga tctatctcca gtattagaag
     9301 aagaagggct aggggcaatc acgttcagtc cgcttgctca agggttgctg acaaatcgtt
     9361 atctacatgg tattcctgaa gattctagag cacatcgtaa ggaaatacca ttcctttcag
     9421 aagaacaagt tggatcaact ctggaaaaaa tcaaagcact gcaaacaatc gccgtatcac
     9481 gaggacaatc attagcgcaa atagcccttg catggaattt acgacaaaaa tctgttacaa
     9541 gtgtactgat cggagcaagt cggttaagtc agttacaaga aagtgttcga atgatggata
     9601 atttagattt ctcaccagaa gaagaattga gaatcgacca aattctagaa tagaaacgat
     9661 gtaatcaaac tttttatcca tatattaaaa aaaacaaaag atgacatcaa gaatcgaatg
     9721 aagatcgtct gttattgaac atcatatttt ttgtcgatca aaataaaaat gatcgaaggc
     9781 taggagtgaa ttttatccta gtctttttta tggtaaatat cttcttcgtt tgtatgtcta
     9841 tgaacggatt aatcgatttt ttatacaaaa aatcatcaga tgattcaaac gtgttataat
     9901 aattacgaaa aaatacatca gatgaaaagg aggtatgtgg tttggaagaa aataaaaccc
     9961 tagtagaagt aactactgac aagatcctta gatatttaaa ggaaatgcag ttgcaagtag
    10021 gagataaact gccgaatgaa tatgaattgt ccgattattt agaggtgggg cgaagcacgg
    10081 tgcgagaagc tgtgcgggct ctagcttcca ggaatatcct tgaagttcgg caaggagcgg
    10141 ggacatttgt cagtgataag cgagggattg ccaatgaccc actcggtttt tctttgattg
    10201 aagatcagga tcggatgata cgagacttat ttgaactgag atatttattg gagcctaaga
    10261 tggcaggttt ggcagcggaa tatgcaacga agaatcaaat cgtccaaatg aaaaaattaa
    10321 cgcaaaagat tgaaacttct tttcgagaag gcacacaaga acatgttcaa ttagacatcg
    10381 attttcatac gatgattgca gaagctagcg gaaacatagc cttgctgcat attatcccaa
    10441 tcattaatga atcgattgct ttgtttaata aaaattatga tatccagcat ctgaaaatcg
    10501 agacgatcga tattcataca gagatcgttc aggccatcga aaggcggaac gtacttgctg
    10561 cgtttgacgc aatgactgtc catatggcaa taaatcggag agaattacag aaaataatag
    10621 gagaaaaaga aactaattga cagttcatct gatgtatttt atgataaata catcagatga
    10681 ttttttgttt tatatttggt aaaaaagagg agtaaatagt ttctgttcac caaaatgtaa
    10741 gcgctgacta atagacaaaa agagatgacg tcaaggagaa gaagcaggaa tgaggggatc
    10801 tggaatgagt tttatggata aattagccaa agttttaggg aacgtggcaa cgagaatcaa
    10861 taatttaagg tatatcatgg tgataaaaaa cgcatttgct gcattgattc cggtaattat
    10921 tacgggtgct tttggcacat tgttttcagc aatggtcttt gactcggaaa atggcttagc
    10981 acagataagt tacttgcgtt ttttagaaga attaaagcct atcgcatctg ccatcagcta
    11041 tgtcacacta agtttcttga ccatctatgc tgtttttcta atagggatcg aattagccaa
    11101 gctgaataaa gtggatggtg tattccctgg tatcatcgca gtgatgtctt atttatctgt
    11161 taatccaact gtttatgaat tagtgaatga aaatcagact gttatagtag aaaacgtatt
    11221 ggctaaacaa tatacagaca cgaaagtggt gttagtatca aatcttagac agcttttcgt
    11281 agagacaaga ataaattaaa cttatctacg agaggatgaa tttttatgac ccgatatgaa
    11341 gaaaatttta aacaaatgat tattgaactg aatcaaactg gacgttctgt tcgagggtta
    11401 gcgaaagaat atggcttatc tgaagcaacg atttacaaat ggaagaattt atatttacct
    11461 gatcagtcca caggactgac tggaaaagaa gtagctgaac tgagaaaaga aaatgctcgt
    11521 ttaaatgagg aacttgaaat cttaaaaaaa gccgcagcca tattctctcg gaaaacctaa
    11581 attcgcttgt tcaatttatt gaaaaatggt gtaaggatta cactgtttct ttgttgtgtc
    11641 ggttattaga aatccctaga agtgtctatt atttttataa gaataaaccg ttgacagcta
    11701 cagaaatcag aaataataag ttgaaaaaga agatttcaac aatttttttt acgaataaac
    11761 aacgctatgg tgccacaaag atccatcaag ttttattaaa agaagggatt tcagtatctc
    11821 ttaaacatgt ccaaaagcta atgaaacaat taaattta
//
LOCUS       DAZKNO010000083        11643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000083 DAZKNO010000000
VERSION     DAZKNO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.084"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..393)
                     /locus_tag="ACG8MY_001669"
     CDS             complement(64..393)
                     /locus_tag="ACG8MY_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0430858.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            570..1298
                     /gene="sfsA"
                     /locus_tag="ACG8MY_001670"
     CDS             570..1298
                     /gene="sfsA"
                     /locus_tag="ACG8MY_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0430859.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1314..2327
                     /locus_tag="ACG8MY_001671"
     CDS             1314..2327
                     /locus_tag="ACG8MY_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0430860.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2327..3604
                     /locus_tag="ACG8MY_001672"
     CDS             2327..3604
                     /locus_tag="ACG8MY_001672"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0430861.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3667..6369
                     /locus_tag="ACG8MY_001673"
     CDS             3667..6369
                     /locus_tag="ACG8MY_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0430862.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6521..6838
                     /locus_tag="ACG8MY_001674"
     CDS             6521..6838
                     /locus_tag="ACG8MY_001674"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0430863.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6868..7188
                     /locus_tag="ACG8MY_001675"
     CDS             6868..7188
                     /locus_tag="ACG8MY_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0430864.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7296..8756
                     /locus_tag="ACG8MY_001676"
     CDS             7296..8756
                     /locus_tag="ACG8MY_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0430865.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8824..9045
                     /locus_tag="ACG8MY_001677"
     CDS             8824..9045
                     /locus_tag="ACG8MY_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430866.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9076..9258
                     /locus_tag="ACG8MY_001678"
     CDS             9076..9258
                     /locus_tag="ACG8MY_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0430867.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9258..9671
                     /locus_tag="ACG8MY_001679"
     CDS             9258..9671
                     /locus_tag="ACG8MY_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0430868.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9794..10975
                     /locus_tag="ACG8MY_001680"
     CDS             9794..10975
                     /locus_tag="ACG8MY_001680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0430869.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(11046..11210)
                     /locus_tag="ACG8MY_001681"
     CDS             complement(11046..11210)
                     /locus_tag="ACG8MY_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430870.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11364..11437
                     /locus_tag="ACG8MY_001682"
     tRNA            11364..11437
                     /locus_tag="ACG8MY_001682"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11398..11400,aa:Arg,seq:cct)
BASE COUNT         3825 a         1961 c         2455 g         3402 t
ORIGIN      
        1 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
       61 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
      121 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
      181 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
      241 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
      301 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
      361 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
      421 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
      481 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
      541 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
      601 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
      661 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
      721 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
      781 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
      841 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
      901 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
      961 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
     1021 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
     1081 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
     1141 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
     1201 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
     1261 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
     1321 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
     1381 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
     1441 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
     1501 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
     1561 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
     1621 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
     1681 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
     1741 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
     1801 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
     1861 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
     1921 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
     1981 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
     2041 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
     2101 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
     2161 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
     2221 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
     2281 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
     2341 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
     2401 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
     2461 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
     2521 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
     2581 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
     2641 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
     2701 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
     2761 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
     2821 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
     2881 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
     2941 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
     3001 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
     3061 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
     3121 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
     3181 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
     3241 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
     3301 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
     3361 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
     3421 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
     3481 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
     3541 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
     3601 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
     3661 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
     3721 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
     3781 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
     3841 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
     3901 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
     3961 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
     4021 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
     4081 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
     4141 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
     4201 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
     4261 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
     4321 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
     4381 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
     4441 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
     4501 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
     4561 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
     4621 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
     4681 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
     4741 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
     4801 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
     4861 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
     4921 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
     4981 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
     5041 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
     5101 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
     5161 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
     5221 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
     5281 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
     5341 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
     5401 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
     5461 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
     5521 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
     5581 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
     5641 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
     5701 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
     5761 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
     5821 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
     5881 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
     5941 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
     6001 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
     6061 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
     6121 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
     6181 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
     6241 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
     6301 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
     6361 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
     6421 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
     6481 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
     6541 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
     6601 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
     6661 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
     6721 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
     6781 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
     6841 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
     6901 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
     6961 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
     7021 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
     7081 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
     7141 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
     7201 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
     7261 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
     7321 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
     7381 gtgatgcttt tatcggcata ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
     7441 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
     7501 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
     7561 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
     7621 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
     7681 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
     7741 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
     7801 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
     7861 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
     7921 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
     7981 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
     8041 aactgatcat tgatttggtg caaaaatata ttgcagaacc attcctagga ttatctcaag
     8101 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
     8161 atggatcaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
     8221 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
     8281 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
     8341 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
     8401 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
     8461 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
     8521 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
     8581 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
     8641 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
     8701 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
     8761 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
     8821 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
     8881 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
     8941 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
     9001 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
     9061 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
     9121 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
     9181 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
     9241 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
     9301 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
     9361 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
     9421 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
     9481 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
     9541 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
     9601 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
     9661 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
     9721 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
     9781 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
     9841 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
     9901 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
     9961 aagtcaagta atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    10021 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    10081 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    10141 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    10201 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    10261 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    10321 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    10381 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    10441 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    10501 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    10561 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    10621 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    10681 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    10741 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    10801 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    10861 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    10921 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    10981 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    11041 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    11101 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    11161 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    11221 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    11281 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    11341 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    11401 aagcgatagg tcgctggttc aattccagtc aggaacgtat aaataacccc tgtatccttt
    11461 gtggtacagg ggtttgtttt gtatttgtgc caaaaatgtg ccaaactagt tatcattttt
    11521 atataaacta tcgaatacag cattgatttt ttctttatcc tgatcttcaa gttctcgaac
    11581 gatatgaaga taagtagaca ttgtagtttc taaactgcta tgtcctaacc gttttgatac
    11641 gct
//
LOCUS       DAZKNO010000084        11416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000084 DAZKNO010000000
VERSION     DAZKNO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.085"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..597)
                     /locus_tag="ACG8MY_001683"
     CDS             complement(112..597)
                     /locus_tag="ACG8MY_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0430871.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    687..1032
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1077..1718
                     /locus_tag="ACG8MY_001684"
     CDS             1077..1718
                     /locus_tag="ACG8MY_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0430872.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1737..2372
                     /locus_tag="ACG8MY_001685"
     CDS             1737..2372
                     /locus_tag="ACG8MY_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0430873.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2390..3205
                     /locus_tag="ACG8MY_001686"
     CDS             2390..3205
                     /locus_tag="ACG8MY_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0430874.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3524..4960
                     /locus_tag="ACG8MY_001687"
     CDS             3524..4960
                     /locus_tag="ACG8MY_001687"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0430875.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5149..7596
                     /locus_tag="ACG8MY_001688"
     CDS             5149..7596
                     /locus_tag="ACG8MY_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0430876.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7826..8893
                     /gene="rlmN"
                     /locus_tag="ACG8MY_001689"
     CDS             7826..8893
                     /gene="rlmN"
                     /locus_tag="ACG8MY_001689"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0430877.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8883..9089
                     /locus_tag="ACG8MY_001690"
     CDS             8883..9089
                     /locus_tag="ACG8MY_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430878.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9086..10189)
                     /locus_tag="ACG8MY_001691"
     CDS             complement(9086..10189)
                     /locus_tag="ACG8MY_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0430879.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10742..11290
                     /gene="aac(6')"
                     /locus_tag="ACG8MY_001692"
     CDS             10742..11290
                     /gene="aac(6')"
                     /locus_tag="ACG8MY_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0430880.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3787 a         1935 c         2468 g         3226 t
ORIGIN      
        1 cgttcatact agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc
       61 taaataaaga aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt
      121 ttcataaact ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat
      181 aatcaacgta gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc
      241 agcatcgatc acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac
      301 tgtacctgct ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg
      361 cttttctttt aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat
      421 gacaagatag atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc
      481 aggtagggaa aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa
      541 agagataccc agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc
      601 gcatcaccct ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat
      661 catgtaaatt acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt
      721 gtttttagag agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg
      781 gagtcgattt tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg
      841 ttgctagatg accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac
      901 cacgtgatta acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc
      961 aacgactgtg tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa
     1021 tacgtcctct tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg
     1081 agtatttgat tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat
     1141 ttttatttac cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt
     1201 tgcattttaa gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac
     1261 cgctgttgtt gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg
     1321 atcgtttcga agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga
     1381 tctttcgtgg agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc
     1441 aattaagtcc ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt
     1501 taccttctgt agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat
     1561 taggtttagg tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac
     1621 tgcctttgct aggcgtggca atcatctatc ttacattgac tggtattcta acaatcgtaa
     1681 tgaaacaagt tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga
     1741 ttgaattgaa gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc
     1801 taatcattcc agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca
     1861 cgcttttacg tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg
     1921 gtgtaccgtt caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc
     1981 aagacttcca gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa
     2041 tggtattgaa acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat
     2101 tagggattag tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc
     2161 gggtagcgat tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa
     2221 caagtgcttt agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag
     2281 aagatggggt aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg
     2341 atcaattact aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac
     2401 cagttttcta ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa
     2461 aacttcagca gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga
     2521 cgatacattt gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat
     2581 tgatttagcc aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga
     2641 ctggtcaatg aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta
     2701 ttccaaaacg ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga
     2761 tcaagtgctg atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa
     2821 gagattaggt gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt
     2881 attaaaagag atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt
     2941 gatcgatctg aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta
     3001 ttatttgaaa caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag
     3061 tgaggatttt gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga
     3121 agccctagat caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg
     3181 cgaagatgtt acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt
     3241 ccctttttcc gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc
     3301 aggctcacaa cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat
     3361 ttatgcaaaa atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg
     3421 actttttcac aacctccttt acatattttt attttgttgt ggaaggggtt tcgtgttatt
     3481 ctaaaagagg aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact
     3541 tacctcattt ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg
     3601 agcttggcaa gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg
     3661 aaagacattt aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga
     3721 agatattgct ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac
     3781 acggattttt cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag
     3841 attgatcgac gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga
     3901 tcttccacaa gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga
     3961 tttcactcat tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat
     4021 tagtttgaat gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc
     4081 aggtgtgaca gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc
     4141 atctgtcatc aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc
     4201 gtatacgcca aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc
     4261 agaagacctg atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc
     4321 tgccatgagg ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga
     4381 attgctagaa tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa
     4441 tgcattcaat ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg
     4501 ttcatcagaa gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga
     4561 acgaacaaat tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat
     4621 cacaagccgt taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa
     4681 gttgacagaa gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt
     4741 caaagccatc aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc
     4801 ttatacagat ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt
     4861 tgatcaagac gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg
     4921 gtaccaaaaa ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga
     4981 aggtacggct ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa
     5041 ctgtaaaata agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc
     5101 ccttactttt ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag
     5161 aaacggccta ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag
     5221 gaacaaatgc tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt
     5281 ttaaaattag gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat
     5341 acttaccaaa tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat
     5401 acagagtttt caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt
     5461 atcttattat tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc
     5521 atggggacga catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac
     5581 gttggtggtg ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag
     5641 cctggaagtt atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat
     5701 ctggaaggct atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta
     5761 ttggaaggtg atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa
     5821 cagcgcgcag aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa
     5881 gctgcagaat atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa
     5941 tggcaggaac cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt
     6001 caagaaaata tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc
     6061 aatacagcag aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt
     6121 gagattgaag cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat
     6181 tctataccaa ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta
     6241 ttagaacaaa ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga
     6301 ccatccgtca caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg
     6361 aacttaacag atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg
     6421 attccaggta aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc
     6481 tttagagaaa tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta
     6541 ggacgtgatg tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc
     6601 gtggctggtt cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata
     6661 ttaatgcgtg ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa
     6721 ttgaatatgt acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa
     6781 gcagcgcaag ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg
     6841 gcaaccggag taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa
     6901 aacggaacga aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat
     6961 ttgatgatgg tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct
     7021 cgggcagcgg ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca
     7081 ggaatcatca aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat
     7141 tctcggacga tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta
     7201 ttcttgccaa tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac
     7261 gaagtagaac gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa
     7321 atgatgccga cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg
     7381 tttgaagaag caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa
     7441 agaagatttc ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat
     7501 ggagttgttg gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa
     7561 acatcagaaa cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa
     7621 aacgaataac caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc
     7681 catcttttct aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta
     7741 ttcgatttat ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa
     7801 ctttgagaaa aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga
     7861 agaattaatc aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg
     7921 ggaatggttg tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca
     7981 attgatcgaa aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca
     8041 agaagcttcg gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga
     8101 gactgtactg atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg
     8161 taatatcggc tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc
     8221 tggagaaatt gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg
     8281 cgaacgcgtt tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa
     8341 tgtaatggac tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca
     8401 cattacagtt tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt
     8461 gcaagtcaat ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat
     8521 gcggatcaac cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga
     8581 gaaaacaaac cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc
     8641 agagcatgcg caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa
     8701 cttgattcca tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga
     8761 tgtattgaga ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga
     8821 acatggtaca gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac
     8881 agttgccaaa taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct
     8941 taattaagga actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc
     9001 tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct
     9061 gcttgatggg ggttttagca attatttaac tgattttctt atatccttgc tggctttctt
     9121 gtgtagcatg ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt
     9181 cataaggcac atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat
     9241 cggtcgtgac attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa
     9301 acacatagat tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac
     9361 cataactgac agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt
     9421 aatagaatga gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc
     9481 cggtagtaac aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg
     9541 cactatcttt caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga
     9601 tttggattcc taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt
     9661 cgacttcttt ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg
     9721 caataattgc tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt
     9781 ccattacgcc ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac
     9841 caatagtggg aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat
     9901 cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct
     9961 ggatcaactg ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa
    10021 cctctatttc ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct
    10081 tccctccaat cagcactact tttgtcccat attctttgac gattccattt gctttttcat
    10141 acgcatctgt tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct
    10201 tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag
    10261 atggagtaga ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg
    10321 aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat
    10381 gaatttttgt tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa
    10441 aattctaaaa attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt
    10501 tttttttaaa gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat
    10561 tgatggcttt cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt
    10621 tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag
    10681 cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg
    10741 tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt
    10801 actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat
    10861 gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat
    10921 tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag
    10981 ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc
    11041 ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt
    11101 aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg
    11161 tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa
    11221 tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa
    11281 ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat
    11341 ttacgaaaat tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt
    11401 ttggaacacc gtgtac
//
LOCUS       DAZKNO010000085        11151 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000085 DAZKNO010000000
VERSION     DAZKNO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.086"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1475)
                     /locus_tag="ACG8MY_001693"
     CDS             complement(<1..1475)
                     /locus_tag="ACG8MY_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0430881.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDP"
     gene            complement(1468..2919)
                     /locus_tag="ACG8MY_001694"
     CDS             complement(1468..2919)
                     /locus_tag="ACG8MY_001694"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0430882.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(2933..3949)
                     /locus_tag="ACG8MY_001695"
     CDS             complement(2933..3949)
                     /locus_tag="ACG8MY_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0430883.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            4169..5257
                     /gene="chvE"
                     /locus_tag="ACG8MY_001696"
     CDS             4169..5257
                     /gene="chvE"
                     /locus_tag="ACG8MY_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0430884.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            5311..6837
                     /gene="mmsA"
                     /locus_tag="ACG8MY_001697"
     CDS             5311..6837
                     /gene="mmsA"
                     /locus_tag="ACG8MY_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0430885.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            6839..8044
                     /gene="mmsB"
                     /locus_tag="ACG8MY_001698"
     CDS             6839..8044
                     /gene="mmsB"
                     /locus_tag="ACG8MY_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0430886.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            8301..9278
                     /gene="guaC"
                     /locus_tag="ACG8MY_001699"
     CDS             8301..9278
                     /gene="guaC"
                     /locus_tag="ACG8MY_001699"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0430887.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            9542..10966
                     /locus_tag="ACG8MY_001700"
     CDS             9542..10966
                     /locus_tag="ACG8MY_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0430888.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         3536 a         1879 c         2424 g         3312 t
ORIGIN      
        1 gggtcattat aaccaatttc aaaagcaata tctgaaatac gatagtctga ttgctgcaat
       61 aatttttttg ctaattcgat tctttgttct gtcagataat cgatgaatgt ttggttaaac
      121 gattgagaaa aaatcgtact gaaatgagag ggacttaatg caacttgttg agcgacccaa
      181 gttaaggaca tatctggatc ggtaaaattt tctttgataa aatccaatgc atggttcaaa
      241 attggctgat atttgttcct tgaaggctgt gagggcagag cagttaagaa agaaatgaac
      301 atataaactg tcgaacggta aagcgagtcg tcggcgctga ttgctaagcg ttgactggtg
      361 gatgtgcaat gaaccatttc ttgcgggatt ttcttttctt tcaggcagac atgctggagt
      421 agcttagtca agacaaataa acgatataat cgtgttcgag ttggtgtgcc ttgtggtttc
      481 gtcaagtgtt cgatgtactt agatacttcg tttttcgata atgaagacaa atcaagtgag
      541 aggtcaaagt aaaagacaga atcaactgga agtttcgctt cttgaatcaa atcctcatag
      601 ctgataattt gttctttaag ggaactggcg gtttcaagta tcatctgcgt gatctcaaaa
      661 gatgcaggta attcgctgat tctttctgcg acaggaccaa tggcgacaga aaaattattt
      721 ttttgtccct tttctaattc atggaccaac gtttgggcta cctgataact ggtctcgatc
      781 acattttctt caagagaatc aactattaaa aatttgatga atcgagagga aacagcagag
      841 aagatcactt gttgattctc cctaaataaa aacttcaaat aatcactgag atgagcgtaa
      901 tcttcaaagt ctgtactgac ttggtttgct gctaataata cggtcatttt tttccctgct
      961 aaagagcgat tgaaagattc agcaagtttc aatgtttcac tgatcgtaag tgagcctttg
     1021 aacaaaccgt tcaagaaatg attttttttc aattcgaaaa caagatcaga agaagtgtgc
     1081 gtttcttttt gctgcaaatc taaagcagtt actgcttttt ctattgcttg tttcaattcg
     1141 ctacttttaa tcggtttaag cagataatca tctgcctgta cctggattgc tcctttcata
     1201 taatcgaaat cgtcgaatcc actaataaat atgagacgag tccaaggaag tagtttcctt
     1261 gcttcttttg aaaaagtcaa cccgtccatg aaaggcattt ggatatcagt cagaatgatg
     1321 tcgggctgta agtcgataat tgaagacagt gccatctcac catctccagc ttcgccgaca
     1381 taagtgattg gataataaga accaaggaca gttatttgtt cccgcaagtt attgcgaatg
     1441 aggtattcgt cttcgatgat aaaaacttta tacatgtttg tttcctccaa ttgttttggg
     1501 agatagaggg atcatcagtg tgacagttgt tccttctcgg tatttgctgt caatcgtgat
     1561 aggtttttga tcatttccgt aataaagaag gagacgcttg tttacgttgt aaagtccgta
     1621 gccgatactg aaatctgaat cgatcccttt ggctaactct ttacgtattt ctgccagttt
     1681 ttctgcaggg atgccaatcc cgttgtctat aactttgaat tggatcatct catcaatttt
     1741 gcatgtggta atacgaataa atcctttgtg acggacgtac ttcgttccat ggtatacagc
     1801 attttcaatc aatggctgca agatcatttt taaaatagaa acaggaaaaa gtgtttctgg
     1861 tatatcgatc ttatagtcaa gcatatggcc gtatcttatt tttaagatga ctagataatc
     1921 attgatatgg ctaatttctt tttcaagagg aatccagtct tgtcctttgc ttaaagacgt
     1981 acgaaaaaag ccggaaagtg catgggtcat agtcagagct tcttcaaagc agtcttgttc
     2041 gatcaaagaa ataatggcat ccaaactgtt ataaataaaa tggggagtaa tctgagcttg
     2101 caatacacga acttcacttt gtgctaaatg gtattgtttc aaagcgttct cccgtaaaga
     2161 ctggtttaga tcttccgcca ttttattcat gctagcggta agtgtttgta attccagtgt
     2221 ttccggcagg gttaaacgaa agccaaggtt ccctgaagaa agttgttctg acatctcgat
     2281 cagctgattg atcggttctt ggacctttct ctctaaaaag cgacgattct ttttcgcata
     2341 gaaaaaaatg gtactaagaa ctccaagttc cactattgtt aatagaatga gcgagcggac
     2401 aagtgtcgta ttttttttgc ttgctaagtc gatctccaca cgaacaaaat cttgtaagat
     2461 atctgacagc agttgagtga cagaatctac ctgattgatg atctcttcat tggtatccat
     2521 aaaatctttt gccaagatat tattgatcag ctcgttttga taatcttcca gtgtatcgat
     2581 cgtacgtagc gatacatcta acgtactaat ttctttaagt gtatttgtat tatttttgat
     2641 ctctcgaata tcattcctaa gttcttctag gatattttcc tcattgaact catctacggt
     2701 tacttggcca gaaatcaagt cccacatact ttccaaaact ttttgatcaa ccttcaaagc
     2761 aattgcatct gctttttgga tatttgcgat cgtctgttga taaatcaaaa gctgttgcga
     2821 atataacagt atgctcagta gcaaagggat aacagcaaat agaagcatgg ctttgtttgc
     2881 ttgggcaaaa atctgtttta tgcttaaatt ttttgtcatg atacttctcc agtcaataat
     2941 ttctggttgg gatgttagct atattctgct gggaaaagac agtctcggaa ataaaaatat
     3001 ctttaggtaa tgtttttcct gtttgaatat ttccaaaata acgagtaacg gtattggcta
     3061 cataccagcc gacatcaggt ttacactcga ctacacaatt gacgattcct gagcgtagct
     3121 tctcgatcat atcttcttgt ccatcaatag ttacaatgat caaatcttct ccaggtttga
     3181 ttgatgtttc ttttatcgca gctaaagcac cgtgagtcat ttcatcacta tggctgtata
     3241 atacatcgat ttcggataaa tcatttgttt ctaaatatcg tttcatctgg tcttttcctt
     3301 tcatccgaat aaaatcaccg acaattcggt gaacaatagt catcggaggt ttatggtcga
     3361 tggttccatc tttttttagg gttggtttac gattgattga ttccaaaaat ccttcggagc
     3421 gtagcttggt cggtgtggag ttctctgaac cagcaagttc taggattctg atttcctgtt
     3481 tctttgaatt tgtgaagtga ttgctgacaa ataaacccgc ccgatttcct tctgctttga
     3541 aactggggcc aatgtgggtg acgaaaagct cgggatcatt cgtttgaata tgtctgtcga
     3601 caagtatgac agggatccct gctcgctttg cttcttttaa tacagagtcc cacccggatt
     3661 cttgcaaagg agtaaaaatg atcatatcca ctttataggc aatgaacgtc cgtatatctt
     3721 ggatttgccg ttcctgattc atgtagccat ttcggtagag aacttggtaa tcttcttttt
     3781 caagcgcagt gataattgat tcagtatgct ttttccgcca gctactctcc gttcctgatt
     3841 gcgaaaaacc aacgactagt gggtcaggtg cttcctttgt attaggtgta caccccatca
     3901 tttggtaagc gattacaaca agggctaaaa aaaataaaac acgtttcact ttaaatcact
     3961 ccatctggat ttgttagtac tatcttatca gataaaaggg aaaatcaaag tcaaaaggaa
     4021 tcaatcgaaa aaaatccaag aaacttaaaa aaatccaaga caaaaaaagt acacctaaaa
     4081 aatttaaagt aaatcccata aaaaaatcaa tgttaacgct ttctttatta gctatactcg
     4141 gtgtatcaat tgaaatgggg gataaaaaat gaaaaacaaa tttagaaaga ttgttgggac
     4201 tatcgctatt ttgaccgcaa gcattctatt aagtgcgtgt ggcaattcag gatctgcaga
     4261 tgatagtacg gggtatgtag gaatcgctat gccgaccaaa tcagcagaaa gatggatcgc
     4321 tgatgggaac aacatggtct cggaattaga aaaactgggt tataaaacag atcttcaata
     4381 tggagaagat aaagtagaaa atcaagtagc acaaatagaa aatatgatta caaaaggggt
     4441 agatacactt gtgattgctt ctatcgatgg ttcggcttta actgatgtgt tagctaaagc
     4501 aaaagaagca gatatcaagg tgatcgccta tgatcgttta ctgatgaatt cagaaaatgt
     4561 cgattattac gcgacttttg ataattttgg agtaggtgta tcgcaagcag cttatattga
     4621 agagcactta ggattgaaag aaggaaaagg accattcacg attgaattat ttggtggctc
     4681 gcctgacgac aacaacgcac taatcaatta caacggagtg atgtctgttt tacaaccata
     4741 tatggacaac ggtcagttgg tcgtgccatc tggacagacg agtttcagtc aaatcgctac
     4801 gcttcgctgg gatggttcta cggcgcaagc aaggatggat aatctgttaa gcgcaaatta
     4861 tactgaccaa acattggatg ctgttttgtc gccatatgat ccaataagtt taggaatcat
     4921 ttcgtcatta aaaggcgtgg ggtatgggag tgaaagtaaa cctttacctg tgattactgg
     4981 acaagatgcg acagttgctg gcgttaaatc gatcattgcc ggggaacaaa cacaaacgat
     5041 ctttaaagac acacgtatct tagcaaaaaa tacgattgaa atgatcaaag caatcagtga
     5101 tggagaagag gttccggtca atgatactga aacttatgat aatggggtga aaactgtccc
     5161 tacgtattta gcaaatacag tatctgtcga taaagataat tatcaagcag agctgatcga
     5221 caccgattat tataaagaat ccgatctgaa aaattaagag gaaccaaaca aggaactaaa
     5281 gagatctaag tcagagagag gataagaatt atggcggatt gtattttaaa aatggcagat
     5341 atcaccaaaa aattttctgg aatccgagcg ttagattgcg tgaatctgat gattgaacgt
     5401 ggggaaatcc atgcattgtg cggagaaaac ggagcgggaa aatcaacatt gatgaatgtc
     5461 ttgagcggct tgtatccata tggctcctat gacggggaaa tcttttatga tggaaagctg
     5521 tgtcaattta aagatttgaa agatagtgaa gaaaaaggca tcgtcatcat tcatcaagaa
     5581 ttagcactaa gtccttatct ttcaataaaa gagaatattt ttctcggaaa tgaacaagca
     5641 gaaagaggcg tgattgattg ggaactgaca gaaaagaaaa catatgaact gctgaataaa
     5701 gtcggtttgt ctatcgaccc gaatcttcta gtatcccaga ttggcgtagg gcaacagcaa
     5761 ctagttgaaa tagctaaagc attatcaaaa tccgtccgtc tgttgatttt ggatgagcct
     5821 accgctgcat tgaatgaaga agaaagcgcg aatttgctga gattgatccg agagttgaaa
     5881 gagcaaggtg taacctccat cattatttca cataagctga atgaaatcgt ggatatcgca
     5941 gatcgaatca ccattttacg ggatggacag acgattgaga cactagaaaa tgaaatgatc
     6001 agcgaagagc atattattcg cggaatggtg ggcagagatt tagttgatcg ttatcctgaa
     6061 agaaaaccgg atattggaga agtttatttt aaagtagaag actggacggt ctatcaccct
     6121 cttgatcacg agcgaatcat aaacgaccac atcaacttgc atttgaaaaa aggagagatt
     6181 gtaggaatag ctggactgat gggagcgggc agaactgaat tcgctatgag tctatttgga
     6241 cattcttatg gaagcaagat atccggacgt atattaaagg aaggggcaga gatatcagtt
     6301 aagaccgttc cttcagccat tgcaaacgga ctcgcttatg tctcggagga ccgaaaatct
     6361 ttaggactga atctgctgat ggatattcgg gaaaacgtga cgctgagcag tttggataaa
     6421 ataagtcaaa aaggagtatt agataaagaa aaagaagtaa ctgaggcaga aaatttccgg
     6481 aaaaagatgc gtataaaaac aaactccatc catcaaattg tcagtagtct tagtggtggg
     6541 aatcaacaaa aagtcgtttt atccaagtgg ctgatgacac aacccgatat tctttttttg
     6601 gatgagccaa cccgtgggat agatgttggg gcaaaatatg agatctacac gattatcgaa
     6661 gaaatggcag caaatggcaa agctgtctgt cttatctctt ctgaacttcc tgaaatatta
     6721 gggatgtgtg atcgaatcta tacgatgaac gaaggacgta ttacaggaga agtctcgcga
     6781 gaagatgcag atcaggaagt cttgatgaaa ttaatgacga aagaagaggt gggttaagtt
     6841 ggaacaggca aaaaatctta cggataaaaa agagaaaaaa ctaaatttga aaagtagagc
     6901 agctgatctt tttggtcgct acagcatggt gatcatttta gcgggactac ttattgcgtt
     6961 tcaattgatg acggatggta ttttctggcg tccattgaat ataactaacc ttgttctgca
     7021 aaatagccat atattagtac tagctgcagg gatgttatta gttgtgctct taggtcatgt
     7081 ggatttgtcg gtagggtcag tgatggcgtt tgttggtgca attgcgggca tgatgatggt
     7141 caatcgccaa atgaacccgt ggatgacagt tatcgcatgt cttgcagtag gcgggctgat
     7201 cggcgcatgg caaggttttt gggtagctta tgcagggata ccggcgttta tcgtgacttt
     7261 agcaggattg ctgatgtttc gtggactgac acaagttgtt ctaggaggcc aatctctagc
     7321 accttttcca gctgttttcc aaaaaatgtc tacgggttat ttaccagact taagcgaagg
     7381 aagcagcctt cacttcttga cattgatttt aggaatgatc atcacgatcg tgttagttgt
     7441 tggtcaatgg cgaacaagaa atcgtcggaa aaatcattta tttgaagtgg aatctccaac
     7501 agctttcttg gtaaaggcgg tatttacagc agtcgttgta ctaggaataa cttatatctt
     7561 tgcctcttat caagggtatc cgattatttt gatcattctt ggtttgatcg ttgcagcata
     7621 tgggtttttg acaaataaaa cagtagcagg tcgacagatt tatgcgacgg gcggaaatcg
     7681 aaaagcggct gaactatcag ggatcaaaac aaagcgaatc acgttctggg tattcgtcaa
     7741 catgggagtc atggcagctt tagctggatt agttttagct gctcgattga atgcggcaac
     7801 acctcaagca ggtacatcga tggaactgga tgcaatggcg gcagtatatt ttggtggtgc
     7861 ttcgacttcc ggcggcattg gaacaatcat gggtgcgatt gtcggtggct tggtcatggg
     7921 tgtattgaac aatggcatgt cgattttagg cgtcggggta gactggcagc aggcaatcaa
     7981 aggattgatc ttgcttttag ccgttgtgct agacttgtat aataagaaaa aacgaacaac
     8041 gtaggagaac gccggttatt cagaaaaaag actgtgacaa gacatttgtc acagtctttt
     8101 tttcgctaaa agcgaacatt cattgtttcg tttgtataga ctaaaaataa tatatgtttt
     8161 tttaccattc gtataaattc tctttttgct cttgattttt ttgcttaatt gagttaaatt
     8221 acatagggcg ctaaaaaaag ccttgattaa attaataatg ttcgtgtttt gctggatgtg
     8281 aacatcagga ggataatgta atgaaagtat ttgattacga agatattcaa ttaattccta
     8341 ataaatgtat cgtaaatagc cgttctgaat gtgatacaac ggtcacatta ggtaaacata
     8401 cattcaaaat gccagtagtg cctgccaaca tgcagacgat tattgatgaa acaatcgcag
     8461 aattcttagc ggaaaatggt tatttctata tcatgcatcg gtttgatgaa gcagctcgaa
     8521 ttccgtttat caaaaaaatg aaaaagcgtg gattgattac ctcgatcagt gttggcgtca
     8581 agaaagagga atattccttt atagagaagt tggcagaaga atcgctaaat cctgattata
     8641 tcactatcga catcgcgcat ggacatgcta actcagttat tgatatgatt cagcatatta
     8701 aaaaatattt gcctgaaact tttgtgattg ccggtaatgt tggtacaccg gaagccgtcc
     8761 gtgaattgga aaatgcgggc gcagatgcaa caaaagtcgg gattggtcca ggaaaagtat
     8821 gtattacaaa aatcaagaca ggctttggga ctggaggctg gcaactggca gctcttagat
     8881 ggtgtgcgaa agcagctcgc aagccgatca tcgctgacgg tgggatcaga acacatggcg
     8941 atattgcaaa atctgtccgt tttggtgcga caatggtcat gattggttca ttgtttgccg
     9001 gtcacgagga atctcctggc gaaacaaaag tcgaaaatgg aatcgtgtat aaagaatact
     9061 ttggttctgc ttcagaattc caaaaaggcg aaaaacgaaa tgttgaagga aagaaaatct
     9121 ggatccaaca caaaggatct ttgaaagata cactagtgga aatgcaacaa gaccttcaat
     9181 catctatttc ttatgctgga ggaagagatc tggaagccat tcgaaaagtt gactatgtca
     9241 tcgtgaaaaa ttcgattttt aatggagaca caatctaaaa aataaataaa atattaggat
     9301 gttgtgaaaa agttgtcctg cattaagtaa taagaacagc caaacaaaaa tagttcttta
     9361 tatgttttgt ttggctggaa ttattatcgc agatacaagc ttttgaacat cattattcag
     9421 aataagggac tgtgataaga tttttgtcac agtcccttat gttttgcaaa aaaagttttt
     9481 cttttttctc gtaaattcgg taaaatcaaa agaaaaaata gacaatagaa aagagaagct
     9541 tatgtgggaa aaagtagaga aaaaacaagg accgatttat cgtcaaatta tggaacagat
     9601 tatgagagaa atcgaaaacg gaaggatcaa tcctggaagt caacttgaat cagaacgaaa
     9661 actagggcaa ctttttggtg tgaatcgttc caccgttgtc catgcattgg aagagcttag
     9721 ggagcttggg gtactaacga gcaaacgtgg cagcggatgg tttgttaatc agactgaatg
     9781 ggggaaattc gctgttccaa gaatcgattg gcggcaaatg atctctcctc gttatgagca
     9841 gacggactgg tatgagcaaa aaataagaga agcaaagcag atccaaaaaa catcgtttct
     9901 tgatttatac gctagcgaga tgccagatga gcttttgccg aattttgagt ttccttctta
     9961 ttctcttgaa gaaatcttag cggaagaaaa agaaatggat cttttaggct atactccgtt
    10021 acgtaacaaa atcaaacggt atttggaaag taaatttgaa ttggcatttt cccccaacca
    10081 gcttcttgtg actggtggcg gtcagcaagc aatctttttg attttacaaa cgattctttc
    10141 agctggagag gggatagctg tagagtcacc ttcttttttt taccgattac ctcttttcaa
    10201 agcttcagga acgcgtttat ttgggatacc gatggatgaa gaagggatcg acttagaaaa
    10261 actgaaagaa tcgatccata aaaataaatt gaaagcagtg cttatcaacc cgaattttca
    10321 aaaccctaca ggaaaagtga tgagtcaaaa acgacgagaa aagctagtag aattgtgtcg
    10381 acagtatcgg cttccaatta ttgaagacga tgtatttacg gatctttcat ttctaaacca
    10441 agaagaagtc cttcctatac gaacgattga tccggataat gtgttatacg tcggttcgtt
    10501 atctagggtt ttaggaaaaa caacgaaaat cggttgggtg ataggacctg agacttttat
    10561 ctcgcaacta gctcaagcgc aagaaatgat ggagttttcg atgaatatcc ttacacaaat
    10621 cacagtggct aatgtattta atgaagcaat tgatttgaaa atgcaagatg taagaaaaaa
    10681 gttgaaagaa aaaagccgac tgataagcgc ttggggagat tcacaatcgt ttttcaagct
    10741 tcattcaatt ttaggtggtt actatgcctg gattacttgg gatgggaaaa aactagaccc
    10801 cgaactagca gaaaaagtag tagatatggg tcttggaatt gcaccaagtc tactttttgg
    10861 gcaagatata aatggaatca gaatcaatta cagtaggctg gatgaaaacc aaataccgtt
    10921 gttttattca aagatgaaaa ttctagaaaa gtggttaaca gaataatttc acaaagtaaa
    10981 tagattaaag aatataacag cttggattta aatttcaagc tgtttctttt ttttcggaaa
    11041 aagaatacga tataaagaaa ctaagaaaaa accattattt gtacgtccgt tcacgatgtg
    11101 taaataaaat aaatgtttgc aacaaaactg tataacacca acgtttttaa a
//
LOCUS       DAZKNO010000086        11072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000086 DAZKNO010000000
VERSION     DAZKNO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.087"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..816
                     /gene="purC"
                     /locus_tag="ACG8MY_001701"
     CDS             88..816
                     /gene="purC"
                     /locus_tag="ACG8MY_001701"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0430889.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            816..1070
                     /gene="purS"
                     /locus_tag="ACG8MY_001702"
     CDS             816..1070
                     /gene="purS"
                     /locus_tag="ACG8MY_001702"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0430890.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1072..1743
                     /gene="purQ"
                     /locus_tag="ACG8MY_001703"
     CDS             1072..1743
                     /gene="purQ"
                     /locus_tag="ACG8MY_001703"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0430891.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1744..3966
                     /gene="purL"
                     /locus_tag="ACG8MY_001704"
     CDS             1744..3966
                     /gene="purL"
                     /locus_tag="ACG8MY_001704"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0430892.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3951..5390
                     /gene="purF"
                     /locus_tag="ACG8MY_001705"
     CDS             3951..5390
                     /gene="purF"
                     /locus_tag="ACG8MY_001705"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0430893.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5413..6465
                     /gene="purM"
                     /locus_tag="ACG8MY_001706"
     CDS             5413..6465
                     /gene="purM"
                     /locus_tag="ACG8MY_001706"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0430894.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6462..7040
                     /gene="purN"
                     /locus_tag="ACG8MY_001707"
     CDS             6462..7040
                     /gene="purN"
                     /locus_tag="ACG8MY_001707"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0430895.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7037..8572
                     /gene="purH"
                     /locus_tag="ACG8MY_001708"
     CDS             7037..8572
                     /gene="purH"
                     /locus_tag="ACG8MY_001708"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0430896.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8588..9862
                     /gene="purD"
                     /locus_tag="ACG8MY_001709"
     CDS             8588..9862
                     /gene="purD"
                     /locus_tag="ACG8MY_001709"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0430897.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9984..10898
                     /locus_tag="ACG8MY_001710"
     CDS             9984..10898
                     /locus_tag="ACG8MY_001710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0430898.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
BASE COUNT         3647 a         1865 c         2635 g         2925 t
ORIGIN      
        1 aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt
       61 taatgtaaat aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa
      121 gccaaaaaaa tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa
      181 gcaactgcgt tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat
      241 caaatcacaa gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt
      301 gaacaattgt ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg
      361 gttatccgaa atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa
      421 gaattcatca aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt
      481 atcaatgaag agcatatcta tttcttacaa cttgcaaagc cggaagaaat cagtgagctg
      541 aaagcccaag cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg
      601 gttttagtag atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat
      661 gaagtaacac cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa
      721 gacgtttacc gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt
      781 ctatcggaac taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa
      841 cctataaaga ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta
      901 tgggatatga aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg
      961 atgcagaaga accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg
     1021 taaatatgga aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt
     1081 gcggtcatcg tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag
     1141 atcatgggcg ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat
     1201 ggggttttac tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct
     1261 cggttttcta caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc
     1321 ggtacatgca atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga
     1381 cgaaatgagt ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg
     1441 gcctttactt cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa
     1501 ggaaattatt tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc
     1561 tttacttata atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa
     1621 gcagggaatg tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga
     1681 tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta
     1741 tagatgaaaa cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc
     1801 gaatggggat tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg
     1861 cctaattata cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa
     1921 aattctaaac cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct
     1981 ggagaaggcg caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa
     2041 agtcacaatc atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga
     2101 atcatcagag atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg
     2161 ttcggcgaat tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc
     2221 ggcggatacg gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct
     2281 tgttatgaag gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat
     2341 atccagaaag ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca
     2401 ggtcgcgacg gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa
     2461 cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg
     2521 tgtttggaat taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca
     2581 gcaggacttg tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg
     2641 acattagatg aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg
     2701 gaatcacaag aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa
     2761 ctatttcaga agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa
     2821 tatcggctat atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa
     2881 gatgcaccag tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag
     2941 ttagctcctt atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag
     3001 caaccgacga ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc
     3061 aatacagttg tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa
     3121 gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga
     3181 ggacaaatag cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg
     3241 gcgatcaccg actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta
     3301 tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca
     3361 ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt
     3421 ggtatggtcg gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca
     3481 ggggatctaa tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa
     3541 aagatgcagc ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag
     3601 agaaaccaag agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac
     3661 tgttcagaag gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg
     3721 atatctgtac agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt
     3781 gtgttgacag tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct
     3841 gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag
     3901 atcaaaatag aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg
     3961 aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg
     4021 ctagtgtggc atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta
     4081 tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg
     4141 tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac
     4201 gttatgcaac agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc
     4261 atgatcagtc gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa
     4321 aagagttaga ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc
     4381 atttgatccg acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg
     4441 aagtcaaagg tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg
     4501 acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg
     4561 cctcggaaac ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg
     4621 gtgagatagt gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc
     4681 agctttctat ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag
     4741 ggatcaatgt gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag
     4801 taaaagcaga tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg
     4861 cggaagctag tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa
     4921 catttatcca gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg
     4981 tacgaggagt agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta
     5041 cgacaagcag acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga
     5101 ggattgcttc tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta
     5161 aagaactcat tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt
     5221 ctttaggttt tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc
     5281 cgtattcagg attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact
     5341 atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca
     5401 agaagggagt atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa
     5461 gctggatatg aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt
     5521 atgatgggcg ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa
     5581 gagccagtcc ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa
     5641 gcagacaaac acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta
     5701 gcacaaggcg cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg
     5761 ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg
     5821 ctgattggcg gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta
     5881 gccggatttg ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac
     5941 ccaggagatg tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt
     6001 gttcgcaaaa ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg
     6061 aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt
     6121 ccgctgatca aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta
     6181 gaaaatatcc cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg
     6241 ccgattcttc caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg
     6301 tatgaaatct tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa
     6361 gaaatagtac acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc
     6421 gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg
     6481 cttctggaaa cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg
     6541 aatcttcgat tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa
     6601 ctgcgttgag tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt
     6661 atgaagaagc aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg
     6721 gatatatgcg aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata
     6781 tccatccgtc tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag
     6841 ctggagtaaa agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac
     6901 caatcatccg gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag
     6961 aaaaaatcca tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa
     7021 atggaggcta tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt
     7081 agcattcgcc caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa
     7141 gcatgcgttg gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc
     7201 tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg
     7261 aagacgcgat ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga
     7321 ctttgtttgt gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat
     7381 agcagaagct attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa
     7441 aaaccatgaa tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga
     7501 attacgtcag tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt
     7561 tcgtcatact gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga
     7621 aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga
     7681 aaatagccat caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc
     7741 agcagcaaaa caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc
     7801 tgcgctacgg atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa
     7861 tccttgtggt attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc
     7921 agatcctgtt tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac
     7981 agcagaacag atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga
     8041 agctttggaa ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca
     8101 ggaagaaaat caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga
     8161 tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac
     8221 tccagcagaa gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa
     8281 tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg
     8341 agtcggttca gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt
     8401 agctagcgat gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat
     8461 aaaagcaatc attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa
     8521 taaatacgat ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt
     8581 cggataaatg aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa
     8641 acttttagcc agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac
     8701 aaaagatggt atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt
     8761 tgttaagaaa gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg
     8821 gatcgtcgat gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc
     8881 tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac
     8941 agctaaatcc agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg
     9001 tgcgccaatc gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac
     9061 cacagtgaag gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga
     9121 tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc
     9181 gtttgtccgt aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt
     9241 tgataacgat aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat
     9301 ttcaaaaaag atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat
     9361 gaaagaacta ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga
     9421 aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct
     9481 ttctcggttg aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt
     9541 agagttaaag tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta
     9601 ccccgaagaa tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt
     9661 ttactatgct ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta
     9721 tttagtagaa gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt
     9781 aatggataaa acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa
     9841 tagcgaactg atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata
     9901 atcggaagcg ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg
     9961 tatataaata aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag
    10021 cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga
    10081 ttgagacagc ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg
    10141 gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc
    10201 tttttattca aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac
    10261 atatcattga atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt
    10321 tacttcttca tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg
    10381 aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa
    10441 tcgaattgct gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct
    10501 tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta
    10561 tcgatcatga cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat
    10621 ggtcaccata ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg
    10681 aattaaacca aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg
    10741 cttctgcttg gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg
    10801 tagggcgaat caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt
    10861 acgaattgta ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa
    10921 agggctataa attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct
    10981 gtcttttttt attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt
    11041 ttgtttttaa ggaaaagctg tgtccgatat tc
//
LOCUS       DAZKNO010000087        10679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000087 DAZKNO010000000
VERSION     DAZKNO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.088"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..572
                     /locus_tag="ACG8MY_001711"
     CDS             <1..572
                     /locus_tag="ACG8MY_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0430899.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="ACG8MY_001712"
     CDS             576..1217
                     /locus_tag="ACG8MY_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0430900.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="ACG8MY_001713"
     CDS             1214..2131
                     /locus_tag="ACG8MY_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0430901.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="ACG8MY_001714"
     CDS             2128..2799
                     /locus_tag="ACG8MY_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0430902.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="ACG8MY_001715"
     CDS             complement(3340..3828)
                     /locus_tag="ACG8MY_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0430903.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="ACG8MY_001716"
     CDS             complement(4029..4295)
                     /locus_tag="ACG8MY_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0430904.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="ACG8MY_001717"
     CDS             complement(4295..4558)
                     /locus_tag="ACG8MY_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0430905.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5079..5219
                     /locus_tag="ACG8MY_001718"
                     /pseudo
     CDS             5079..5219
                     /locus_tag="ACG8MY_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..7184)
                     /locus_tag="ACG8MY_001719"
     CDS             complement(5361..7184)
                     /locus_tag="ACG8MY_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0430906.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="ACG8MY_001720"
     CDS             complement(7782..10487)
                     /locus_tag="ACG8MY_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0430907.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3069 a         2218 c         2026 g         3366 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataa
//
LOCUS       DAZKNO010000088        10510 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000088 DAZKNO010000000
VERSION     DAZKNO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.089"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(220..972)
                     /locus_tag="ACG8MY_001721"
     CDS             complement(220..972)
                     /locus_tag="ACG8MY_001721"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0430908.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2029
                     /locus_tag="ACG8MY_001722"
     CDS             1085..2029
                     /locus_tag="ACG8MY_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0430909.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQLCTRLARLY"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="ACG8MY_001723"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="ACG8MY_001723"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0430910.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAAFAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="ACG8MY_001724"
     CDS             3311..4789
                     /locus_tag="ACG8MY_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0430911.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="ACG8MY_001725"
     CDS             5066..7126
                     /locus_tag="ACG8MY_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0430912.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSNVYEKDYSQFEGKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="ACG8MY_001726"
     CDS             7209..7778
                     /locus_tag="ACG8MY_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0430913.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="ACG8MY_001727"
     CDS             complement(7953..8759)
                     /locus_tag="ACG8MY_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430914.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="ACG8MY_001728"
     CDS             8897..10411
                     /locus_tag="ACG8MY_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0430915.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3726 a         1752 c         2070 g         2962 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgtacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttt gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat gacgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc taatgtatat gaaaaagatt attcacagtt
     5581 tgaaggaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag
//
LOCUS       DAZKNO010000089        10505 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000089 DAZKNO010000000
VERSION     DAZKNO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.090"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..272
                     /locus_tag="ACG8MY_001729"
     CDS             48..272
                     /locus_tag="ACG8MY_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0430916.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(477..797)
                     /locus_tag="ACG8MY_001730"
     CDS             complement(477..797)
                     /locus_tag="ACG8MY_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0430917.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(945..3179)
                     /locus_tag="ACG8MY_001731"
     CDS             complement(945..3179)
                     /locus_tag="ACG8MY_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0430918.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGISVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3658..3924
                     /locus_tag="ACG8MY_001732"
     CDS             3658..3924
                     /locus_tag="ACG8MY_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0430919.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3924..5651
                     /gene="ptsP"
                     /locus_tag="ACG8MY_001733"
     CDS             3924..5651
                     /gene="ptsP"
                     /locus_tag="ACG8MY_001733"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0430920.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5815..6861
                     /locus_tag="ACG8MY_001734"
     CDS             5815..6861
                     /locus_tag="ACG8MY_001734"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0430921.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6878..8101
                     /locus_tag="ACG8MY_001735"
     CDS             6878..8101
                     /locus_tag="ACG8MY_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0430922.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            8190..9077
                     /locus_tag="ACG8MY_001736"
     CDS             8190..9077
                     /locus_tag="ACG8MY_001736"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0430923.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            9163..9393
                     /locus_tag="ACG8MY_001737"
     CDS             9163..9393
                     /locus_tag="ACG8MY_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0430924.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9450..10415)
                     /locus_tag="ACG8MY_001738"
     CDS             complement(9450..10415)
                     /locus_tag="ACG8MY_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0430925.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNKKEAEKQLN"
BASE COUNT         3401 a         1836 c         2127 g         3141 t
ORIGIN      
        1 aaaaattatt ttgcgtacat atgattaaag aaaggagagt gtgacacatg gaaacagaat
       61 ggcaaagacg tgaaagaaaa aaacagaaaa aagaagagat gttacgatta tgtcgattat
      121 tagatgtaaa agactgggac aaaaacgagc aaattgcaca aaaagtacga tcaatcatcc
      181 ataaagagga aaaagttaaa aataaagaca cgcgtagaac tgaaaatgaa aagagaaaag
      241 tacaaaagaa aagagaaaaa tcaaaaagct agaaagaaaa agaggctgag acagaagcgt
      301 ttaactccaa gaaataagct ggaattcaca aaaatttgaa aagcaatttt cgtgaatttt
      361 agcttatttc cgaaggagtt gcttctgctt ccgccgttta tacgtgttta gagcgtgaga
      421 caaaagtgtt ctttactttt gtcccacgct ctttaattat tatgttcaaa atttgtttac
      481 acggctaatg tagcgcctaa ttcatgtttt ggaatcgaga tttcaacaag gcctgccaat
      541 tcgattactt tgatttcatc ctcgtggtaa acggcgtctt tgattttatc taagaaataa
      601 tttttagcat cttcgatttc tttcggctgg attttacgtt tgtcattgat cagcagaatt
      661 tcattatgtt caggggcttc agtgtagaca atagtggtat ttccggctgt gtagaccttg
      721 actagatggg catcagtgtt ttcaagctgg tttaatacaa gacgggaatg gctgtttgtt
      781 acatttacca atttcatggc gctcacctct tttatgtatt tttaattgct tgtttctctt
      841 attataattt cttttttata aaatgaacag tattttgact tttctgaaaa aagaatgaga
      901 cggcatttct caaagaaata ccgtctcacg ctataaaact ggatttattc agccgtttct
      961 tcggctgatt ctttagccaa acgttctggt ttagaagtaa cgatgatttt tccatcttta
     1021 tccagttttg ctttcaactc atggatagaa gggtgatcaa gataaaattc agcgatacca
     1081 tcttcgattt gttcttggat cgtgcgtcga agaggacgag cacccataga tggatcatag
     1141 cctaagtcaa ctaatttttc tttgacattc gtaggaactt cgatgtgaag ttgctgtgct
     1201 gccagcattt ggttgacatc gtctagcatt agtgtgacga ttgtcatcaa gttttctttt
     1261 gataatgcac tgaattcgat gatcccgtca aaacggttca gaaattctgg tgtaaagtag
     1321 ttgtttaatt ggttcaatac agaacgagta acaccttcgc gtgccgcacc aaaaccaacg
     1381 tttgcttcta ctttaccagt tccggcatta cttgtcataa tgatgatcgt atctttaaag
     1441 ctgaccgttc ttccttgtgc atctgtcaat cgaccatcat ctaagatttg caagaacata
     1501 tgcaaaacat ctggatgagc tttttctact tcatctaaca agacaagact gtaaggattt
     1561 ctccgtactt tttctgttaa ttgaccagct tcttcgtatc cgacataacc tggaggagaa
     1621 ccaatcaatt tagagacact atgtttttcc atatattcag acatatcgaa acgaatcatt
     1681 gaatcttgtg aaccgaatag ttcgtaggca agttgtttcg caagctctgt tttacctaca
     1741 ccagttggtc caacaaataa gaaggagccg attggacgat tctgtttgtt taatccaaca
     1801 cggtttcggc gaatcgcttt cgctactcga tctacagcgt tatcttgtcc gatgacatga
     1861 gctttcaagt catctgctaa gtttttcagt tgtgtctgtt ctttttcttt taattcaccc
     1921 actgaaatac ctgtccgttg ttcaacgatt ttttccatat ctttttcggt aatcacaggt
     1981 gtttcctctt cagtgagctg acgttctttc attttttgaa gtttattgat ctgatcgcga
     2041 taataagctg ctttttcgaa atcttcttct cgagaagcta aaactttttg ttcttctgct
     2101 tcttgcagtt ttttctcgat tgttttagga tcaacgattt ggatcgtcaa gtttttctta
     2161 gaacctgttt catctagcaa atcgatggct ttatccggca agaagcgatc ttggacgtaa
     2221 cgattggata aagttgcggc tgcttcgatg gcctcatcag tatattttac atgatgatag
     2281 tcttcataac gtttttgcaa acctttcaaa atactgatcg tttcttcgac agttggttca
     2341 tctacccgta caggttgcat acgacgttct aaagcagcgt ctttttcgat gatgcggtac
     2401 tcatttaagg tagtagcacc aaccatttgc aattctcctc gagctaaagc tggttttaaa
     2461 atattaccag catccatatt tccatcacca gcagaaccag caccgacgat ttcgtgaact
     2521 tcatctatga ataggatcac attttctgct tgacggatct cttcaatcaa tttttgcata
     2581 cgttcttcaa attgtcctcg tataccagta ccttggacta aagatacgac atctaaacga
     2641 atgacttctt tgtccattaa tttttgcggt acatcaccat ctacgatttt ttgtgctaaa
     2701 ccttcaacaa ctgctgtttt accaacacct ggttctccaa tcaagacagg attgttcttg
     2761 gttcggcgat ttaaaatttc aatgactcgt ttgatttcgt catcgcgtcc gatcacagga
     2821 tcgatatcac cttgtcgtgc ttgttctgtg atattgattc cgtattctcc caataaacct
     2881 tctgtattgc cttgaggtgg ctgaccacca ttgaagttgt tgtttccgcc aaattgtgtt
     2941 ggtggtgttt gctcataatt tccttgctgt tgcatttggc gagacatgga acggaaaaga
     3001 tcgtccaaac tgccaaatcc aaatggatca ttttgggcca tattagtcag acctccattt
     3061 tgttgatttt tgattttttg ataacagctc tgacagtaat cgagttgtgt ccgctgacca
     3121 ttgacagtgg catacaagtg aatcgttgcc tcattcttgc cgcagttttg acaaagcatg
     3181 aatcattcac gtcctttcac aaaaatgggt acatccatat tttacatctc gtttttcatg
     3241 aggtaaaaga ataaccccta gttttttaag agatttatga ggtagaaaag gtgaaatctc
     3301 tattgaccat tcctgacctt tgactcttat tatactaaaa ataaaaaggg aagcaagtaa
     3361 ttcgtttcat atgaaaacaa agagggtcaa aggaaagaat gataaacgtc aggagaagag
     3421 aaagcgacaa gaaaaagaaa ataaacacgt ttgttataaa atgtgaagaa aaggatgaaa
     3481 aagaatccgg aataatttga atacttttat ctgaaattga atgaaaactg ttgtatctaa
     3541 cgaaaaaaaa tgttaatctt tacagatgaa agggtggcat ttgttaggat tctctgaatt
     3601 actaaaaaag aaaaccctca cataaaaaca acactcactt aaaggagacc gattaatatg
     3661 gaaaagaaag aatttcacgt agtagcagaa actgggatcc acgctcgtcc agctacacta
     3721 ttagtacaaa cagcaagcaa atttaactct gatgtaaact tagagtataa aggtaaatca
     3781 gtaaacttga aatcaatcat gggcgttatg tctttaggtg taggtcaagg ttctgatgtt
     3841 actattactg cagaaggtgc tgacgaagct gatgcaatgg cagcaatcgt tgaaacaatg
     3901 aagaaagaag gcttatctga ataatggttg aaatgctaaa agggattgcc gctagtgacg
     3961 gtgtagccgt tgcaaaagct tacctgctag ttcaaccgga tttaacattc agtaaggcaa
     4021 cagtagaaga tactgcggct gaagaagctc gtttggatgc tgcattagct aaatctactg
     4081 aagaattgca gcaaattcgt gaaaaagcag cgcaaagctt aggtgaagca gaagcgcaag
     4141 tatttgatgc ccatttgatg gttctttcag atccagaaat ggtaggtcaa atcaagcaaa
     4201 atattaaaga caacagcgta aatgctgaat cagcgctaaa agaagtaacg gatatgtata
     4261 tcggtatgtt cgaagcaatg gaagataatg cttatatgca agagcgtgct gcagatattc
     4321 gtgatgttgc taaacgtatt ttagcgcact tattaggcgt tactttacca aatccatcaa
     4381 tgatcaacga agaagtggtc gttgttgcac atgacttgac tccaagtgat acagcacaat
     4441 tagatcgcaa ttttgtaaaa gcgtttgtaa cagacattgg tggacgtact tcacattctg
     4501 ctatcatggc acgttcttta gaaattccag caatcgttgg tacaaaagaa attactgcta
     4561 aagtcaaaga aggcgatatt ttggctgtca acgggatcga aggcgatgta atcatcgatc
     4621 ctacagatga acaaaaagct gaatttgaaa aagcaggtgc agattacgca gcacaaaaag
     4681 cagaatggga aaaattaaaa aatgcagaaa ctgtcacagc tgacggcaag cattttgaat
     4741 tagctgcaaa catcggaaca ccaaaagatc tagttggtgt ccataataat ggtggagaag
     4801 cagttggtct gtaccgtact gaattcctgt atatggattc tccagacttc ccaacagaag
     4861 atgaccaata tgaagcttac aaagctgtat tagaaggtat ggaaggaaaa cctgtagttg
     4921 ttcgtacaat ggacatcggt ggagataaag aactaccata tctgcaatta cctcatgaaa
     4981 tgaatccatt cttaggatat cgtgcattgc gtatcagttt atctgaacaa ggcgacgaaa
     5041 tgttccgtac acaaatgcgt gcattgcttc gtgcatctgt tcatggtaac ttacgtatca
     5101 tgttccctat ggtagctaca ctcaaagaat tccgcgcagc aaaagcgatc tttgaagaag
     5161 aaaaacaaaa attgatcagt gaaggcaagg aagtatctga tacgatccaa gtcggcatca
     5221 tgatcgagat tccagcagca gcagtcttag cagacaagtt tgcaaaagaa gtagatttct
     5281 tctctgtagg tacgaacgat ttgattcaat acacaatggc agcagaccgt atgaacgaac
     5341 gcgtttctta cttgtaccaa ccatataacc catcaatttt acgtttgatc aaaaatgtaa
     5401 ttgatgcagc acacgctgaa ggaaaatggg caggtatgtg tggagaaatg gctggagatc
     5461 aaacagctgt tccattgcta gtaggtatgg gtctagatga gttctcaatg agtgcgacat
     5521 ctattcttaa aacgcgtagt ttaatgaaac gcttggacac agcgaaaatg gctgaacttg
     5581 ctgatcgtgc attgaaagaa tgcgatacga tggaagaagt cgttgaactt gttcatgaat
     5641 atgtaaaata aacaacgtat caaaagctgg tgtggcttgt aaaagagcta cgccagtttt
     5701 tttgttcttt tttaagaaaa cgtttttgtc agtaagaata gataaaaata gggcttaagt
     5761 ttgagtgaca gtttgtaggc tatgttatac tatataagtg aagggggacg taaggtgaag
     5821 gtattattat attttgaaag tgaaaagatg cttgcaaaat ctggaatcgg gcgtgcgctt
     5881 gatcatcaaa aacgtgctct gacggaagtc ggtatctctt atacactaga tgaaaaagaa
     5941 gattatgaca tcctgcacat caatacatac ggaatcaata gtcataatat ggtcaataaa
     6001 gccagaagag aaggaaaaaa agttgtctat catgcgcatt ctacagagga agatttccga
     6061 aattccttta tcggatcgaa ccagctttct cctatcgtaa aaaaatatct ggtaggattg
     6121 taccaaaaag cggactatct gatcacgcca acaccttatt caaaacagtt gttggaaagc
     6181 tatagtatcc gtgtgccgat tcaagctatt tccaatggga tcgacttaga gaaatatcgc
     6241 cctgatccaa tgaaagaaca aaagttcaga gaatatttca aactgtctcc ggatcaaaaa
     6301 gtaatcattt gtgtcggttt gtttttcgaa cgtaaaggaa tcattgattt cgtagaaatt
     6361 gctaagaaga tgcccgaata cacatttatt tggtttggtc atgttcccat gtactcgatt
     6421 cctcggaaca tccggaaaat tgtaaaagaa gatcatcctg aaaatgtcct gtttcctggt
     6481 tacattcgtg gagagatcat agaaggtgcg tattcaggtg cagatctatt ctttttccct
     6541 tcttatgaag agacagaagg gatcgttgtt ttagaagctt tagcaagcaa acaaaacgta
     6601 cttgttcgag atattccagt gtataatggc tggttagaag atagcaagaa ctgctatatg
     6661 gggaagacaa acgaagaatt tatccgactg atccaacaga ttaccaatca agaactaccg
     6721 agtacgacac aagccggtta tcaaacagca aaagagcgaa gtatcaataa aatcggtgaa
     6781 gaacttaaaa atgtatacga aagtgtactg aatgaaaaag tctctagcaa attaaaaaaa
     6841 gtaaatcagg tcaaggacta gtttggaggc gaaaaacatg aaaatcggct tttttaccga
     6901 tacctacttt ccgcaagtca gcggagttgc tacatcgatc aaaacattaa aacaagaatt
     6961 agaaagacat gggcatgaag tctatatttt tacaacgacc gatcctaatg cggataagtt
     7021 ggaaaaagat gtgattcgaa tgccaagtgt accatttatt tctttcaagg atcgaagagt
     7081 tgttgttaga ggcatgtggt atgcttattt ggttgccaaa gaactagaac tagatcttat
     7141 tcatacccat acagaattcg gagcagggat acttgggaag atggtagcta agaagttgaa
     7201 aatacctctg atccatactt accatacgat gtatgaagac taccttcatt atatcggtaa
     7261 aggaaaagtg atcaggcaat cccatgtaaa atacttgtct agattatttg caaatcatac
     7321 gacaggtgta gtttgtccaa gtgaacgtgt tattgataca ttacgtagct atggtgtaat
     7381 agcaccgctt cgcgtgattc ctactggtat cgacattgaa aaattcaaac gttctgatat
     7441 cactcagcag gatatcagca aattaaggga gtcactaggt cttcaggaaa accatttgat
     7501 gattctttct ttaagcagga tttcttatga gaaaaatatc caagccttga ttaatgggtt
     7561 tcctcaggtc atctccgctt atcctaacgc cagattagtt atcgtaggaa aaggacctta
     7621 tgtagaagag ttagaggaat tgatctcaga actgcagtta gaagaatttg tgcagttcac
     7681 aggagaagtg gacaacaatg atgtagcact ttattataaa gcggcagatt attttgtcag
     7741 tgcttctact tcagaaacac aagggcttac ctatacagaa gcgatggcag ccggaacacc
     7801 ttgtgtcgtg gaaggaaatg cttatttgaa ccgattgttc gatcatcctt cattagggaa
     7861 aacttttgaa accgacgaag actttgcacc agcacttatt gattatattt ccagtgaagt
     7921 tcctaaagat ccaaaagttt tagaagctaa actttatgat atttcatcta cacatttcgg
     7981 cgaagctatg atcgaatttt atcaagacat ggttgtttat catgaagaag agatgcgaag
     8041 aaaagaagaa gaagactcga ttgaacgaat caaaataaaa ttaaattcat ttaaaagata
     8101 aaattcaaac attttttatg aaaaaatcct tgtttctagc gtatactagc atagaagcaa
     8161 ggatttttga tttttaggag gtcgaaaaaa tgaaacttgg ttttatcgga acaggcgtca
     8221 tgggaagtgc tgtcgcacgc catctactag aagcaggaca cgaagtagct gtctataatc
     8281 ggacaaaagc aaaagctgat cctttagtaa cagaaggagc tatctgggca gatacaccaa
     8341 aagcggtagc agaacaaagt aatattctct ttacaatggt aggttacccc aaagatgtag
     8401 aagaaatcta ctatggacag tcggggattt tttctgcaga tatctcagga catattttag
     8461 ttgacttgac tacaagtaca ccaagcctgg cagaaaaaat tgcaaaaaca gcaaaagaaa
     8521 aaggcgcaga tgcgttagat gcaccggtat ccggtggtga cttaggagca aagaatggta
     8581 cactgacgat catggtcggt ggagaagaag ccgtttatga tcaagtactg cctttattca
     8641 aagaatttgg tacgacattt acattgcatg gttctgctgg gaaaggtcag catacaaaaa
     8701 tggctaatca gatcatgatt gcaggtacga tgacaggaat gacggaaatg cttgtctatg
     8761 cacaaaaaaa cggacttgat ttaaagaaag tgattgagac gctttcaggt ggaagtgcag
     8821 ctaactggtc tttaagcaat tattctcctc gtattttaaa agaagattac acacctggtt
     8881 tttttgtcaa acattttatc aaagatttaa agattgcatt agaagaagca gaaaaaatgg
     8941 accttgtatt gccagcaact acccaagcat tgaaactata tgaagagttg gcagataaag
     9001 gttttgagaa tgacggaacc caagctttga tcaaactttg gtggcctgaa ggaaaaatac
     9061 cagaaaaaaa atcataaaaa acgagttttt ttagagaaaa tcaatgcaag taggctatgc
     9121 ttttgataca atataacaga aataagaaaa ggaggacctc cgatgacaca ttcacaatta
     9181 gtcgcaatca taaaacgact ggaggctatg gtcgagtcag cagataatga actgcaagtt
     9241 cgccgttttg aaaaagaagg cgtagagaag tgtacagtaa cttatgataa agcgaccgat
     9301 acatttgaat taactgaaac agatactcat cagcaatacg aatttgataa tatcgatatc
     9361 gtagcgatgg aaatctatga cttaatccaa taaattttag gataattgac atttgaagtt
     9421 gaagatgcct aaaagaagcc gagaagcaac taatttagtt gcttttcagc ttcttttttg
     9481 tttaacagtt caatttcaga ctgttctttt tctaattttt tgcggtaact gatcaagatt
     9541 tgtacaggat cgatttcttc ttttgacaat tgacgtttca aagataaatg agcttctttc
     9601 ttttcataat agaagtcagg agaaacgtca gcagaggcaa tcattgtttc tgcaagttga
     9661 ccataacaaa cgaggtagtg ctttttctga ctctcatctg ctgctaatgg catgctgaag
     9721 gtgagcgatg gtgttcttaa aaaatgcttt tgttcctgga tccgtctaat aaaataagta
     9781 actaattcga tatctttttg aagcataccg gctttttcga aattcaactg atagatgaaa
     9841 tgttgttgtc tttttttcaa gtaagttagt agctttttat tttggccttg aaacagccct
     9901 tggagttcct tttttttctg ttcaaaagtc atttcctgta ggtcaggaag ctgggctcgc
     9961 aaagctaaca aactaatttc agacaaccaa ggcatttgat aagtttcttc cattatacgc
    10021 aaaatagacg gcattttttt atagagacga aaaggcccgt atgttcgagc cgtaggagta
    10081 tctgttaata caaatccctt attggtaata tgtacataat tataattact gaaataattc
    10141 atttgtcggt tataaaaagg acggtaatgt tggatcaatt gacactcgag caataaagca
    10201 tctaattccg tgtctactac gacgaaatca aaatctgtta tatgatgaat catacgtaat
    10261 actttttttg aatgctgttt attctgatga aaatagctgc ttactctatt tttgagccgt
    10321 tttgctttac caacatagat gaccttacca tcacggtctt tcataagata gacacccggc
    10381 agcatgggga gagttttcat tttttctttt aacataggat ctccttttac gctttgaata
    10441 aaaagactat aacaaatttt gaaataatgg acaagtttta aggaattcta tagttctgtt
    10501 cacag
//
LOCUS       DAZKNO010000090        10459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000090 DAZKNO010000000
VERSION     DAZKNO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.091"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..1177
                     /locus_tag="ACG8MY_001739"
     CDS             join(36..264,264..1177)
                     /locus_tag="ACG8MY_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0430926.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1412..1864
                     /locus_tag="ACG8MY_001740"
     CDS             1412..1864
                     /locus_tag="ACG8MY_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430927.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1878..2567
                     /locus_tag="ACG8MY_001741"
     CDS             1878..2567
                     /locus_tag="ACG8MY_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430928.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2692..5043
                     /locus_tag="ACG8MY_001742"
     CDS             2692..5043
                     /locus_tag="ACG8MY_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0430929.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5123..5518
                     /locus_tag="ACG8MY_001743"
     CDS             5123..5518
                     /locus_tag="ACG8MY_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430930.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5843..6460
                     /locus_tag="ACG8MY_001744"
     CDS             5843..6460
                     /locus_tag="ACG8MY_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0430931.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6501..6800
                     /locus_tag="ACG8MY_001745"
     CDS             6501..6800
                     /locus_tag="ACG8MY_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0430932.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6834..7001
                     /locus_tag="ACG8MY_001746"
     CDS             6834..7001
                     /locus_tag="ACG8MY_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0430933.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7157..7837)
                     /locus_tag="ACG8MY_001747"
                     /pseudo
     CDS             complement(7157..7837)
                     /locus_tag="ACG8MY_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(8043..8591)
                     /locus_tag="ACG8MY_001748"
     CDS             complement(8043..8591)
                     /locus_tag="ACG8MY_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430934.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(8592..9449)
                     /locus_tag="ACG8MY_001749"
     CDS             complement(8592..9449)
                     /locus_tag="ACG8MY_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0430935.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(9735..10022)
                     /locus_tag="ACG8MY_001750"
     CDS             complement(9735..10022)
                     /locus_tag="ACG8MY_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HFQ0430936.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKTLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(10012..10341)
                     /locus_tag="ACG8MY_001751"
     CDS             complement(10012..10341)
                     /locus_tag="ACG8MY_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0430937.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT         3444 a         1723 c         1917 g         3375 t
ORIGIN      
        1 tagaatctaa ctactaagaa aaggacgtgt ccacgatggc aaaagccaag aaaaaatttg
       61 atgaagactt taagaagatg atcttagatt taaaccagtc tggccaatcg gtcgaagaac
      121 tggcagcgca atatggtatc gctacacaaa cgatttatcg ttggaagaag cttcatacaa
      181 agaatgtagc aaccggcatg actgaggcag aaatcctagc gatgaaaaag gaaatggccc
      241 gcatgcagga ggaaaacact atcctaaaaa aggctttaac catattcgct caaaagtaaa
      301 aatgagtgat gtcttcgaat ttattcaatc aaatgttcat gatcatgacg taaaacaaat
      361 gtgtaccgtt ttggaagcac cgaaatccag ctactatgac tggaagaaaa agaaaccttc
      421 caagcgccaa tcagaaaaca aacgcttaaa ccgcttgatt tcaggaatct actttgaaaa
      481 caagggtatc tatggtgcgc ctaaaattca taagattctt gttggccgtg gcgagacctt
      541 gtccttaaag aaagtacaaa tactgatgag aaaattaggc ttgcgttcag tcaccctcaa
      601 gaagtacaag cctggcaaac aagcgaaagt taaaagtgaa ggacgtaaaa acctgttaaa
      661 ccaagatttt accactacgt cgatcaatca aaaatgggtg acagatatta cctatattca
      721 taccctaaaa gatggctgga cctacctctc cactattcaa gacctccaca caaagaaagt
      781 catcggttgg aagtttggca agcagatgac gaaagaattg gttatcgaaa cccttgacaa
      841 tgcgctctta aaccaaacac cctccgagaa cttgatcatc cactcagatt tgggttctca
      901 gtacactagc gaagcctatg aggcaaagct gaatgaattg aacattcggc actccttcag
      961 tcgtaagggc tgtccctacg ataatgccgg tatcgaatcg tttcacgcct cactcaaaaa
     1021 agaagaagtc tatccatcaa aagcgtatga aaactttgaa gctgcttatt tagcgttatt
     1081 tcaatacatt gaaggatttt acaatcgcaa gcgaatccat agtagtatca actatttaac
     1141 accaaatcag atggaggagt tggcattgca agcttagttc ctagctacca cttacatttt
     1201 tttggcgaga tttccacagg gtttcaactt catcgcttcg atcagcggtc aaacggtttt
     1261 ggtttgaccg ctggtcgaaa cgatgagata ctcacccgct ggaatctcga aaaaattctc
     1321 acaaatcgtg tccgaggtat tgactcaaat ccaccaatta tcttatcgta gatatatatt
     1381 ataaatttaa taagaaagcg aggtgagaat cgtgaaaaaa acgatactaa gtattttttc
     1441 agtatttaca atattaattg ggggctattt tcttttgaat ttagattctg ttaattcaaa
     1501 cgctcaaata aaagatcaaa gagtagctga acataataat aaaaaaatga gtttgaccaa
     1561 aaatatctat tctatgtcag atgaagaatt gtataaagaa tttggtgatt tatccgggga
     1621 acagttagaa gaaagtattc caggaattag tgactcagat aaaattgttg tcttgtctgc
     1681 tggtgggcaa ttaatgggag atggcaaatc cgaattagtt agttataaac aaaatcacag
     1741 acagtttatt gatgttaata ctgattccaa cgctatttcg attaaggatc taaaagagct
     1801 tttaaatagt aaaagaaatg aattaaagca gataaaagaa actaattcag tggaattaga
     1861 gtaaaggaag attcattatg aaaaagattt ttggtgtttc tgttgggcta cttatgatgt
     1921 taacatttgg aaatgcagta tatgctgctg agaaagaagt tatttcagat ccagagcagt
     1981 acgcctcacg atactttgct gattttgaag aagaagataa agtacttgca ttgccagaag
     2041 gtggtttttt aaaaggggaa gctaaaattg tatctatgga taatccagat ataattcaag
     2101 gttcatataa ttcagaaact gatcctgctt cagtttcagt gacagaagca aaaaaaattg
     2161 ttgctgaaaa attcaaaaat gatattgaaa ctactccaat tatgcctcga ggagcatttc
     2221 cacccgatgt tggtactagc ggacaccttc ttgtactagc aggaggagga agctataaat
     2281 cacagccatt tagtggtgcg ggatggcgtt ttggaggtat gggtgtaaaa ccagctgatg
     2341 gtacaggcaa ctttttacgt tggaaatcct acattgacga tggaagagta ggaaatgtaa
     2401 atcaagccaa cgcaactaaa tctggttcta ttcaaggaat ggcattgtat gctcgtgaag
     2461 gattcaaata ctttgcacca aacgggcctg gttggacaat gaatataggt atttattaca
     2521 cttataatcc tgttgcaggt acgtattatg ttgttcaaaa tatttaataa aacagcacaa
     2581 agagatttct ttgtgctgtt ttattatttt tagttgttga caaacactaa aatataaaat
     2641 ataatcaaca cataaacgaa cgtatgttcg gcaagaaagc aggggatttt tatggatgag
     2701 atggttttaa gtactcagaa atggctaaac aagaaataca gtaatgttac agggtttgat
     2761 aaggttccag aaaatgggcg aacagggtgg cctacaatct atggacttat tgaaggacta
     2821 caagtagaac ttggtattac taatctagta gctaattttg gacctaccac tgaaaaaatg
     2881 tacgataatc aggttactcc taaatggggc aaaaatctac ctaaaaatat tgtatttctt
     2941 attcaaggtg ccttttggtg taaggggatt aatccaggtg gcttcgatgg ggtatatact
     3001 ccatatttag atactgctgt aaaagagttg cagactgatg cagggtttaa tggaagcgct
     3061 gtaactggag ttttggatgc gaaatgggcc aaagcgttat ttgatatgag tgcttttgtt
     3121 cttgttcccg gcggagatag taaaattaga aaaatgcagc aatggttaaa tgtaaattat
     3181 ttagaataca cgggaataat gccttgtgat ggtatttatc aaagaaattc aaatcaagca
     3241 ttaatttttg cattacaagc tgaactagga tattctccca gtgaagcaac agggtcttat
     3301 ggtaatggga caactagtaa aacttatcct gtgtctgaag gtaactcggg aaactatgta
     3361 agaataattc aatatggatt atatgttaat ggttattttg aagacggtac attcgatggt
     3421 attttcacta aatatatggg tttagaagta ctggcattcc gaaaatttat ggtattgcct
     3481 gaatatactg aaatagcaga tgttgttgta ataaaaggac tattgtctag cgctggagat
     3541 attagaagat ctgcagatgg cgcagatact tctacgcaat taactagatc tcaaatccaa
     3601 acattggttg ataatgatat aaaaatagtt ggtagatatc ttactggtac tgttggaatt
     3661 ggaaaagatg aacgaaataa gtatctaact acagaggaat tgaataatat tttttctaaa
     3721 aatttatctg tgtttcctat ctatcaagat ggaggtgcag cattagctta ttttacttat
     3781 gatcgtggct tatcagatgg taagaaggct atagatgcag caaaaaatct aaatatacca
     3841 ttaacaacag ttatttattt tgctgttgat ctagacatgt taggggaaga aattcttgca
     3901 tatgctggag aatattttaa gggggtatct gctgtaatgt catatagcgg gtatcaaacg
     3961 ggtgtttacg gaacaagaaa tgtttgttct caaataatca ataacgggta tgcttcgttt
     4021 gcatttgttt cagatatgtc gactggttat agtgggaatt taggttttcc tatgcctaga
     4081 caatggtctt ttgatcaatt tgttgaattt accataggaa gtggcaacgg agctgttggt
     4141 atcgatttaa tcgctacatc gggtagagat agcggattta atgagttatc aaatgatact
     4201 aataatgata attacattgc caaatacaat cagaaggtta taaatcagat ggtacgtgcg
     4261 tatcaatatc tatcacaagc tggtcttgat aatccttgga accctcattt gacattttat
     4321 agatatgtaa attattctgg tttgtcatgg gacatcattt cttctcctgt aacagagcat
     4381 gatagaaaaa tttatgatga atataggaat aaattaacta atagcgaggg cctgtataat
     4441 tattttatag atcctaatac tggttcaata ataggtttgc ctcacttaat tgtgacgtta
     4501 caatcacaga tgtttctagc cgacacaatc aatgatagtg tttctgactt tgcaggttgg
     4561 ttaggagatt taatgacttg ctggggtgaa gttaagaaat taggaataca aatggagcaa
     4621 ggtgtatttg cattagtagg tacggctgca ggtggaacat ttagtctaga agatttatac
     4681 caagatgttg acgcggtaaa tgtttatacc ttagcaaaaa gaaattctga ttcaacagct
     4741 ttatcggctt taaaaacgta ctattgtgat aagggtgcta ttgatagatt taatcaattt
     4801 gttaagaaca gatttggttc ttcagataaa atcgcttcag taacaaatag tttattagaa
     4861 aatgacgaaa cagttgatat cttaggtgta gggataattt catttttcat tgataaaata
     4921 gttaataatt cacttgggac agattttact tatcttgatt ttattggaga tgaggggaaa
     4981 gttgcaaatg gattttctga aaaaattatt caatttcaag aaaatagaag taaacttaat
     5041 taagaatcgt ttactattta aacagtgtcc ttttcctttt taaatatata cgataaaata
     5101 agtatatgaa ttgtaaaagg acatgatgat tatggaagaa atagaatgga ataatctgac
     5161 tcaaaataat aaaaataatt ctggatgtgg aattatcttt atgtttgtta tagttttggg
     5221 aatagtagta cttttaataa ctgtcaagat agtagattac tttgcgaata ctcctgaaga
     5281 acaaatacag agaaaacttc ctgagtttga aagagtacca ccttatgatc caagagataa
     5341 ttcatttcaa gtaaaaaaat taaaagagtc tccaaatgaa atgtggtttg aagttacagt
     5401 tgaatcaagc gacttcaaag aaaaaaagaa agaatacaat tatattgtaa aggcaaaagt
     5461 atcaaaaaga tttattttta accctaaatt agaaatatat agtgttgtaa tagaataata
     5521 gtgttaaaaa aattgtaaaa gttaaagaag tcaaattctg atgtgaaaaa taaaatatac
     5581 catggaagac ataaaagaaa aactggataa ctagtttatt taatggcaga ccgttaaggc
     5641 ctgccatttt tatgtatttt agcggaataa atataatcat gcgggagtgt aaacttactg
     5701 tttcaaaaag tatacttttt gacatgcctt gattttcttt gtttatgtat ggcataataa
     5761 ccacataata caatttgaaa cgtttctgat ttaatctgat tgttttgaaa caattttagt
     5821 gaaaaaaggg tagataaaaa cgatgagaaa atttttttat atccgggtca gtagttatga
     5881 tcaatcgatc gaacgacaac tgatttccgc gaaggaatta ggaatccctg aagaatatat
     5941 atttgtagaa aaagccagcg gcaaagattt taaacgccct gaatatcaat taatgaaacg
     6001 gatgtttcga aaaggagatt tactctatct acaatcatta gatagactag gcagaaataa
     6061 acaaatgatt ttagaagaat ggcgagaatt aactaaggaa aagcagattg atatttctgt
     6121 gttagacatg cctctgttag atactgctag ttacaagcaa ctagatgggt tagagacgct
     6181 tatctctgac ttaattctgc aattactcag ctatatggcc gaagacgagc ggaaacgaat
     6241 taatagccgg caaagagaag gcgtagaagc tgcgaaacaa aagggtgtaa aattcggcag
     6301 aaagaaaatt gctttacctc ctgaatttcc tgcgatctat tctgactgga aagcaggaaa
     6361 gataactgca gtctatgcta tggatcaatt gggcatgaaa cgaaatacct tttatcgacg
     6421 ggtgaaagaa tatgaacata ccttgcacca cttttcttga tagatataac agctattaga
     6481 cgggaaggga gagtttgaaa atggacttaa cgaatcaaag ggtcgttttt actggaacgc
     6541 tgcagcaaat gacacgaacc caagccatcg gtttggttca ctctttaaac ggccattgtc
     6601 aatcaagtgt tacttcaaaa acaaatttcc tggtttgtgg tcagtattct aagtcgttgt
     6661 ttgatgattc tctctataca aaaaaggaac aaaccgcgcg ggacttgatt gcgttaggtc
     6721 atgagattac cattttatca gaagtcgaat tttatgcgtt aattagtcaa cagttgaaag
     6781 aatggcaacg gtatttataa tcgttagtaa aatttacaag gaggaaaatt tttatgaagc
     6841 gaaaatctta tgatcgttgg ggcattgaaa taactagttt taatcgaaat attgcttttc
     6901 acgtggagtg tgactgtggg gaattagcaa aaagtgttta tcaaaaaaat tacttttact
     6961 gtcctaaatg ccatcgccga tacggggtac atggaacata gaaacgaaat agtgcggtag
     7021 aaaagcgaat gaatgtagta tatttctaca aatcattcgc tttctttatt ttataaatag
     7081 ttggtcgaga gatcccgttt tcggttctgt tgcgaagttt gaaaatcaaa cgcgccctct
     7141 ctgaactcca aatatcttag gctgttattc ccattaatac cttgatttca gtagacaccg
     7201 aaaagccgaa gagcgttcca tttcttcggt tctttttata tattcctcga atggtctcca
     7261 tgcccttaat cgtggaagag gctgtacgga gactttgata aaatttattt cgtcgtttaa
     7321 taggtcgatg gtcttgttct attaaattgt taagatactt cacagttcgg tgctctgtct
     7381 tagtatataa acccacactc tgtaactttc taaaggcgga gccaagagaa ggtgctttat
     7441 cggtcacaat tgctttcggc tcaccaaact gtttatggag tcgttttaag aaagcatagg
     7501 ctgcttgcgt atcccgtttc tttcgtaacc agatatctaa ggttaagccg tccgcatcaa
     7561 ttgcacgata aagataatgc caacgtccct taattttgat ataggtttcg tccattttcc
     7621 atgaatagaa ggattgtcta tttttcttct tccaaagata atagaggact ttgctgtact
     7681 cttataccca acgataaatc gtagtatgac aaacatttat tccacgatca tataacaatt
     7741 cctgaacttc acgatagctt agattgtaac gcaggtagta accaacagcg acaataatga
     7801 cgtctttttt gaattgtttg cctttaaaat gattcattac tctgtcctct ctgtcttttt
     7861 tctcaatttt acactaaaat agattttttg gaaaactttg caacagaacc accgttccta
     7921 tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct ctatttcacg
     7981 caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt cttctttgat
     8041 tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc ggtatcttgt
     8101 tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg aacaattgga
     8161 tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc ttgcttatct
     8221 tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa ttcatctctt
     8281 gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa caaagtcgac
     8341 agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg tgcgccaact
     8401 ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat ttcttgaact
     8461 tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt tttctttgct
     8521 tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc aagtcctttg
     8581 atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa ttctttcaaa
     8641 atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc tttctttgtc
     8701 ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt gagtacaaca
     8761 tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc ctgatctact
     8821 gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt tgtatcaaat
     8881 tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat cgattttact
     8941 tgggtctgta agacgacgat tgcccagtcc gcaatgtaca tcgcggattt actatataca
     9001 gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa tggttctagc
     9061 ttttctttca tatatttgaa ttgggacaat gaatcattat attggtcttt gatccaatca
     9121 tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat tactttatag
     9181 acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt ttcgtaaaca
     9241 atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt taagttcatc
     9301 acatcagaag tatttccttg catatccaag tcaattatca aggttctttt tccttcatta
     9361 actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc acctttaaaa
     9421 ttaccgatta catatttttt cgctaccacg ttcctacctc ttttctttta gcaatattca
     9481 tttgatacta ttataacata tttattttta gttactacca aaaaaaagat atatatgtac
     9541 taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga aatatattga
     9601 tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag agaatactcg
     9661 cgactttagt catgtagatg aatcagatct tcgataaaaa agaagccgtc cttcttaaga
     9721 acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc atgcgttcca
     9781 cataacaata ctggaataac cttgttttct ataatctcgt aagctactct atatgttgtg
     9841 cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt ttgttcaccc
     9901 ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc tttcaatgtc
     9961 ttctctttta gcttttttaa atacttaatg acatgttttg aaggaacgat ttcataagcc
    10021 aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg tatcttcagc
    10081 aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta caagaatctc
    10141 atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa aatcataaaa
    10201 ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact ttgcttcaac
    10261 ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg aattgccttg
    10321 ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa ttacttagta
    10381 aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg taatgcgtcc
    10441 ttgctgaata aatttcaac
//
LOCUS       DAZKNO010000091        10429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000091 DAZKNO010000000
VERSION     DAZKNO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.092"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..1143
                     /locus_tag="ACG8MY_001752"
     CDS             4..1143
                     /locus_tag="ACG8MY_001752"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0430938.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1136..1936
                     /locus_tag="ACG8MY_001753"
     CDS             1136..1936
                     /locus_tag="ACG8MY_001753"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0430939.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1936..2763
                     /locus_tag="ACG8MY_001754"
     CDS             1936..2763
                     /locus_tag="ACG8MY_001754"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0430940.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2768..3475
                     /locus_tag="ACG8MY_001755"
     CDS             2768..3475
                     /locus_tag="ACG8MY_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0430941.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3575..4441
                     /gene="rfbA"
                     /locus_tag="ACG8MY_001756"
     CDS             3575..4441
                     /gene="rfbA"
                     /locus_tag="ACG8MY_001756"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0430942.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4455..5027
                     /gene="rfbC"
                     /locus_tag="ACG8MY_001757"
     CDS             4455..5027
                     /gene="rfbC"
                     /locus_tag="ACG8MY_001757"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0430943.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5049..6077
                     /gene="rfbB"
                     /locus_tag="ACG8MY_001758"
     CDS             5049..6077
                     /gene="rfbB"
                     /locus_tag="ACG8MY_001758"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0430944.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6175..7026
                     /gene="rfbD"
                     /locus_tag="ACG8MY_001759"
     CDS             6175..7026
                     /gene="rfbD"
                     /locus_tag="ACG8MY_001759"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0430945.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7060..9093
                     /locus_tag="ACG8MY_001760"
     CDS             7060..9093
                     /locus_tag="ACG8MY_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430946.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9137..10417
                     /locus_tag="ACG8MY_001761"
     CDS             9137..10417
                     /locus_tag="ACG8MY_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0430947.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         3186 a         1750 c         2237 g         3256 t
ORIGIN      
        1 catatgcttg tttcttttag tatggttata agattttttc tgacaatttt attatcactg
       61 atattaacac ctgtattcaa aatcatttca gtacaaacag gtatggtcga caaaccaaat
      121 gaaagaagaa taaataaagt tccaatgcct tcagcgggcg gactgcctat ctttatttca
      181 tttgttatat cgactttatt cttattccgt aaaattatcc ctcaatcgta tattgtaccg
      241 atcttgattg cttctttagt aatcatagtc acaggagtgc tcgatgataa atatgagctg
      301 acacctaaac aaaaaagtat agggatcttg attgcttctc ttattattta ttttgtagca
      361 gatatacgta ttgattcctt cacgcttcct tttattggat atatccaact aggctggctt
      421 agttttcccg taacgatttt ttggatcttc ggtatcacga atgcggtcaa tctgattgac
      481 ggtttggatg ggctggcggc agggatttca ttgatcggtt taattacgat aggaatcatt
      541 ggatattttt tcctccatgc ttctacagtg tatattccag ttgttatttt ctgtcttgta
      601 gcaagtatta tcggtttttt tccttacaat ttttatccgg caaagattta tttaggcgat
      661 acaggagctc tattcctagg gtttatgatg gccgtccttt cactacaagg gctgaagaac
      721 gtgacttttg tttcatcgat ttctttgctg atcgtcatgg gagtgccggt aacagacacg
      781 ttttttgcta tcattaggag aaaagcaaac cgtgtttctt tttctacagc agataaaaaa
      841 catcttcatc accgactaat cagtttaggc tttactcata aaggtgctgt cctgacgatt
      901 tatgcgatgg ctttgatgtt ttctttcaca gctatggtca tgaattatac tggaagaatc
      961 ggaacgatca tattgattat tgccatgttg tttgctgcaa ttttattatt tgaattgatt
     1021 ggtctgatca atgagaaaca tcagtttatt ttaaaaacat tgcgattttt agggaataaa
     1081 gagtaccgta cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca tatagatgaa
     1141 taacaaatta aaccagccga tccttattag tattgtgacg gcaaacagtg ataaaatttt
     1201 tcggacacta gataatttta tttcaactgt aaaagatgaa acagcagtaa aaatccgaat
     1261 ttttgacaac aattcacttc ccgagtacaa aaaaagacta acagagtata ggaaatatcc
     1321 ctacatcgat atctattttc atgatgaaaa ttccggattt ggttatggac ataatcacaa
     1381 tctgatagct agcgattctc gctatggcat tatttgtaac ccagatattt tagtggatga
     1441 agagacagtt acagcattgg tcttgttatt gaaagaacat ccggaatgtg cgatgttagc
     1501 acctaagatt cttaatgaag atggaacaac tcagcacctc atccgtgagc agctggctgt
     1561 ttttgattac gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg
     1621 actggctgtg tttgaatgcc gaggattgcc tactgatgct aatagttatg tacggatgat
     1681 ctcaggtagt tttatggtag ctgatattga taaatttaaa gagataaagg ggtttgatga
     1741 gcgtttcttc atgtactttg aagacaacga cttgtgtttg agttttgaaa aagcaggtta
     1801 caagttattg tacacaccct tttatgcagt tatccatttt tacgggaagg gtgcgcatcg
     1861 aaatgcaaaa ctgtttcgcg tgtttttgac ttctatgatg aaatttttca ataagtgggg
     1921 gtggcgtttt ttctgatgaa accagaaata ttagtagtcg ttgtgcttta tcagcaacta
     1981 ttcagtcaat ctccagctta tggaccatta agcaaagcac ttagcgaaaa gcaaattcag
     2041 cttattattt acgataatag tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat
     2101 atcgtttatc atcatgatcc gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg
     2161 tctcaagcag atgaaggaac acgttgtctc gtcactctgg atcaggatac gaggctagtc
     2221 gacgattatt ttgaactatt gcgtaaagta acctttacag atgaatgtgt tgctgcagtt
     2281 ccgatgattt tttctgggag ccgacaagtt tctcccctat attcagatcg ttatatcaat
     2341 cgtacagctc aagcagtaga agttgaaggc ccaacagctg aacgaatcat ggcaatcaat
     2401 tcaggtgttg cctggtctgt caaatttcta aaaaagattg gcgggtttaa tcctgctttt
     2461 tctttagatt ttttggatca ttggctgttt tggaaagtgg atcagttgga aaaaactgtg
     2521 gagatccttc ctgtacgaat ggaacatgat ctttcggttt tagactatga aaaagtttca
     2581 agtaatcgat accgttctat tttacaagca gaaaatcgat tttatcatga atatgatcaa
     2641 aggcatttat ccagtcaccg acgacaatta ttgttaagaa cagctaaaca gttcttgacc
     2701 gtcaaaaacc ggcatatctg gcggatgact ttgcgctcat atgttaagaa ttggaaggtg
     2761 tgaattcgtg cgttcggttt gtatagctac ctttaatgga ggcagatatt taagagagca
     2821 gttagatagt attttatctc agctttcagc agaagatgaa gtagtcattt ctgatgatgg
     2881 gtcagtagat gatacatggg atatattaac tgaatacgca gaaaaagata agcgaatcca
     2941 gcttttttcg gggcctcgtc aaggattgat cgctaatttt ggttatgcga tccagcagac
     3001 gaaaggagat ctaatctttt tggcagacca agatgatgtt tggttggaga caaaagtgaa
     3061 aaagatcact gactattttg agcagaatcc ggatcagcta gtagtagttt ctgatttaat
     3121 cattgtggac agcgagctgc aagagatcca tgattcgtat ttttcgtttc gaaattccaa
     3181 agaaggatgg cttaataatg tgataagaag tcactatatt ggtgctggta tggctttccg
     3241 gtcttcttta aaacccgata ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg
     3301 gattggtttg ctgggaggaa agaagactgg gttcctaaga gaaccgttaa ctctgtaccg
     3361 aagacatgat ttaaacgcga gtgagattga aacacattct aaattacggc aaaaaatttc
     3421 ttggcgtgtt catctgatta cggcgttgat caaacgaaaa tggtttaaac gttgataaaa
     3481 gatgtttaaa tccttaatat ttccatgaca aatataacaa ttgatgagat aataacaaag
     3541 tgaaatttga aatcaaaaag gagagcagat atccatgaag ggaatcattc ttgctggggg
     3601 cagcggaaca cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga tgccgattta
     3661 tgacaaacca atgatttatt atccaatgtc tactctgatg ttggcgggaa tcaaagaaat
     3721 tctgattatt tcaacgccac aagatacacc acgtttcaag gaattatttg gaaacggaaa
     3781 tgatttagga ctgcagatcg aatatgcagt acaagaaagt ccagatggat tggcacaagc
     3841 gtttatcatt ggagaagaat ttattggaga tgatagtgtc tgcttggtat taggggacaa
     3901 tatttactat ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa aagatacagg
     3961 cgcgacagta tttggctatc atgtcaatga tccagaacgc tttggtgtag tagaatttga
     4021 tgagaatatg caagctttgt cgattgaaga aaagcctgct cagccaaaat caaattacgc
     4081 tgtaactggc ctttattttt atgacaatga ggtaatttct atcgctaaag gtatcaaacc
     4141 ttcagaacgt ggagaactag agattacgga tgtcaataaa gcatatttgg ataaaggaaa
     4201 actatccgta gaacttatgg gacgcggatt tgcttggctt gatacaggaa cacatgaatc
     4261 attgcttgaa gcatctacct ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc
     4321 ttgtttggaa gaaattgctt accgtatggg ctacatcgat aaggaacaat tgctggccct
     4381 tgcacagcca ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg ccgctgaata
     4441 aggagtcctt acagatgaaa gtaacagata caaaattaac agatgtcaaa atcgtagaaa
     4501 tggacgtctt tggcgatcat cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg
     4561 cagaacatgg attgaaatat gactttgttc aagacaacca ttcactatca gctgaagctg
     4621 gtgtccttcg ggggcttcat ttccaaaaag gagaagctgc acagacaaaa ttgattcggg
     4681 tagtcactgg agcagtttta gatgtagtag tggatatccg caaaggtagc ccgacttatg
     4741 gtcaatggga cggatttatc ttatctgaac ataatcatcg acagttgctt gtacctaaag
     4801 gatatgccca tggattcgtt actttgacac caaatgtgaa cttcctttat aagtgtgata
     4861 attactataa tgcagaagca gatgggggca tcgcatttga tgatccagat ttagcgattg
     4921 attggccaat tgatccatca agagcgatta tgtcggaaaa agatcaccgt catccgacat
     4981 taaaagaatt tgaagcagaa aatccatttg tttacggaga aatctaagaa ttaaggagaa
     5041 attttcttat gaaaaacatc attgttaccg gaggtgccgg attcatcggc tctaatttcg
     5101 ttcattacgt ggtaaacaat catccagaag tgcatgtcac tgttttggat aaactgactt
     5161 atgcaggaaa caaagagaac ttggctggac ttcctagcga tcgcgtagaa ttggtcgtag
     5221 gagatgttgc agatgcagaa ttagtggacc gtctagtaaa agaagcggac gcagttgttc
     5281 attacgcggc agaatcccat aacgataatt cattaaaaga tcctttccca tttgtacaaa
     5341 cgaatatcat tggtacgtat acacttttag aagcttgccg caagtatgat gtacgctatc
     5401 atcacgtatc tactgacgag gtttacggcg atcttccatt acgtgaagac ctgccaggac
     5461 atggggaagg cgaaggagaa aaattcactg cggaaacacc atataatcct tcaagtccat
     5521 attcttctac aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt tcttttggct
     5581 tacgtgcaac catttcaaac tgttcaaaca actatggtcc ttatcagcat attgaaaaat
     5641 ttattcctcg ccaagtaacg aatatcttaa gcggtattcg tccaaaatta tatggcgaag
     5701 gaaagaatgt tcgtgactgg atccatacga atgatcattc ttcagcagtg tggctgattt
     5761 tgacgaaagg cagaattggc gaaacctatc tgatcggtgc agacggtgaa gaagataata
     5821 agaccgtgat ggaattgatc ttagaaatga tgggtcaacc aaaagatgcg tacgaccatg
     5881 taaacgaccg tgcaggacac gacttgcgtt atgcaatcga ttcgacaaaa ttacgggaag
     5941 agttaggatg gaagcctgag tttacgaact tccgtgatgg gttggctgac acgatcaaat
     6001 ggtacgaaga acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg
     6061 ccaaaaatgg acaataatac agagaaaatg tcactgtatt aatggaaaaa cacatgtgag
     6121 gcaggcgctc gcctgtctct tttttaaaat aaaaatcaag gagcggataa tcatatgttt
     6181 ttattaactg gaggaaatgg gcaactaggt acagaattgc gccatttatt agatgaaaaa
     6241 aatctgcaat atgtttcaac agatgcaaaa gacttggata tcacagatgc cgagaaaaca
     6301 atggcttaca tcacagaatt aaaacctgat gttattttcc attgtgcagc ttacacagca
     6361 gtagataaag cagaagaaga agcaaaagaa ttagatgaga agatcaatgt tgatggtaca
     6421 agaaatgtag cacttgcagc aaaagctgtg aatgctcaat tcgtctatat cagtacagac
     6481 tatgttttcg atggaaaaaa caaaaatgta gaatacgaag tagacgacca aacgaatcca
     6541 ctaaatgaat atggtcgtac gaaattatta ggcgaaaaag cagtagaaga gattttagaa
     6601 gactattata tcatacgaac ttcttgggtt ttcgggatct atggacacaa tttcgtcttc
     6661 acgatgcaac gcctagcgga aacccgcgac aaactgactg tagtaaatga ccagtttggg
     6721 cgaccaactt ggacacgtac tttggcagaa tttatggtat atgtcatcga agaaaaagca
     6781 ccatttggta tttatcattt gtcgaacgaa aatagctgtt cttggtatga attcgcaaaa
     6841 gaaatattga aagatacaga tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa
     6901 aaagcaacac gtccgcagta ttcagtcatg agcctgaaaa agacagaaga gttaggattt
     6961 gtcataccaa cttggcaaga agcacttgcg caaatgctgg aagctgccaa aaaaatgaat
     7021 aagtaatttc cttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt
     7081 gcacgacgaa aagaaaagcc aaaagcgaac caggcagatt cgaatttggt gcttgacctg
     7141 tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat
     7201 ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa
     7261 aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg gctggtgcag
     7321 acaccttttt acctctcaca agctgaagag gactttccgt tggtgaacga ggattattca
     7381 ttaaacggac aaaacatgat cattgcgtat aattctcctg caaaggatat tacggttatc
     7441 ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat
     7501 tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg
     7561 aagagaaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc
     7621 cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgact
     7681 gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt
     7741 ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg
     7801 ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt
     7861 tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc
     7921 atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat
     7981 ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt
     8041 acaaattgga aaattccttt tgaagatatc aactattcga ataactctga agtggccctg
     8101 ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa
     8161 gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt
     8221 tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct
     8281 ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc
     8341 gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta
     8401 aatgcatggt cttatttcta tgcgattacg cattcgaaaa tagataatta tttttcggat
     8461 tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcatttgc attatttggc
     8521 tggagacgct tgttttacct cgcttttgcc gccttagtta tcgtatctgg ctttttcgtc
     8581 aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa
     8641 aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc
     8701 accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca
     8761 tggaaggaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca
     8821 agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg
     8881 gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga
     8941 ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat
     9001 aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca
     9061 caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat
     9121 attagtagaa aggagagtgg agataattca agcaatcgta caaaaatttg tcaacaaaat
     9181 gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt tttgtttgga cagcagggtt
     9241 aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt
     9301 ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg
     9361 tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt
     9421 atttggagat ttactgtttc ctttgattat tttattttgt ttagtaatta aatggatcga
     9481 gtggacaaaa tgggatcgcg gtatttatct gattgtcttt acggtcttcc tcggaatcac
     9541 gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct gggctccagg
     9601 caattcgctt tctgatattt ggatcaatac gaatacaatc ggaagttctt tgatgactct
     9661 tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgtac gaatcatcgg
     9721 tgcagtagca gttgttgcag cttttctcgg gatttgggtc tgccaatcta gaggtgcatt
     9781 agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat
     9841 tcgtgcgcca tttattgctt acacggttac gattctttta gctttgccga tttcttatct
     9901 ggcggctgtc tctgaaaaag tcaatttgtt taccggtcga gaagatattt ggcataaatt
     9961 ctatcagacg ctaggagaaa aatctgaaca agttttacta ggaatgaaaa catttatctt
    10021 ccaacgagga aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta
    10081 tggattgata ggatttggga ttgctacttt gctgcttatc ctttttattg gtcgcttaac
    10141 gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat
    10201 gatgcagtca tttatggagg atacattgac ttcataccca tgggttccga ttatttatat
    10261 cctgttggct atggccgttc atcgatatga tgaaccagaa gttgcggagg aattctatgt
    10321 tatggaagag gcatatgacg atatgactta tgatgataca tcttatgagg aacaaccaga
    10381 tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaat
//
LOCUS       DAZKNO010000092        10337 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000092 DAZKNO010000000
VERSION     DAZKNO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.093"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..283
                     /locus_tag="ACG8MY_001762"
     CDS             134..283
                     /locus_tag="ACG8MY_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0430948.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            294..1808
                     /gene="dltA"
                     /locus_tag="ACG8MY_001763"
     CDS             294..1808
                     /gene="dltA"
                     /locus_tag="ACG8MY_001763"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0430949.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1805..3010
                     /gene="dltB"
                     /locus_tag="ACG8MY_001764"
     CDS             1805..3010
                     /gene="dltB"
                     /locus_tag="ACG8MY_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0430950.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3055..3288
                     /gene="dltC"
                     /locus_tag="ACG8MY_001765"
     CDS             3055..3288
                     /gene="dltC"
                     /locus_tag="ACG8MY_001765"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0430951.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3291..4559
                     /gene="dltD"
                     /locus_tag="ACG8MY_001766"
     CDS             3291..4559
                     /gene="dltD"
                     /locus_tag="ACG8MY_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0430952.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4700..5782
                     /locus_tag="ACG8MY_001767"
     CDS             4700..5782
                     /locus_tag="ACG8MY_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0430953.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5782..6585
                     /locus_tag="ACG8MY_001768"
     CDS             5782..6585
                     /locus_tag="ACG8MY_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0430954.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6655..7395
                     /locus_tag="ACG8MY_001769"
     CDS             6655..7395
                     /locus_tag="ACG8MY_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0430955.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7406..8410
                     /locus_tag="ACG8MY_001770"
     CDS             7406..8410
                     /locus_tag="ACG8MY_001770"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0430956.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8455..10299)
                     /locus_tag="ACG8MY_001771"
     CDS             complement(8455..10299)
                     /locus_tag="ACG8MY_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430957.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3322 a         1706 c         2088 g         3221 t
ORIGIN      
        1 tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa aacggtttct
       61 gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg aggttatatg
      121 aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt ggggaaaatt
      181 catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc tgtatcacta
      241 taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag agtatggaaa
      301 tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat cgagttgctt
      361 atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac gcacttgctt
      421 attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc aatttggagt
      481 ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat ttgccgattg
      541 aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa ccatctatgg
      601 tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata acaaaaaaag
      661 aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc gtaaaaggta
      721 cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa ggcgtacaaa
      781 tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt gggatcaagc
      841 ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta ttttctattt
      901 atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt gttcaagact
      961 ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt tctactccat
     1021 catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg ccggaattgg
     1081 acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt ctacttgatc
     1141 gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact gtagctattt
     1201 caagtattca gattgaccag catgtactag atagttatga ccgactaccg attggctacg
     1261 taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca aaaggagaaa
     1321 cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac aatcccgaaa
     1381 agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca ggggatgcag
     1441 gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag caggtcaaat
     1501 tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa gacaaccgaa
     1561 tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag ctggttgctt
     1621 ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa tctatcaaag
     1681 aacaattact agaagttgta atggattata tgattcctca gaagttccat tatgtcgatc
     1741 tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct gaggtgaatc
     1801 ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt ttcttttagc
     1861 cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat taccagttta
     1921 tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt attggcaaca
     1981 gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg gctattttgt
     2041 ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt tagcgataat
     2101 tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat cgttacttgg
     2161 ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg aaataagaga
     2221 tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt tctttccaac
     2281 aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc tccatccgcc
     2341 tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt ttgtcggttt
     2401 tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg tagtgggacg
     2461 cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt acgtgtacag
     2521 tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta caagctacat
     2581 gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga atatcaaaga
     2641 attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg tatatatgcg
     2701 attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa tgtcaaatat
     2761 cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt ggtactacat
     2821 cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga tccgatttaa
     2881 gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag ccgtattttt
     2941 gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag ataaactttt
     3001 ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag gaaaatggaa
     3061 gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag aaaaaatcgt
     3121 gatattgatc tatttgatga agggttgctt gattctttag catctgttca attattagtc
     3181 gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg agaggattgg
     3241 agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt atgaaaaaga
     3301 aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt gtgtttttta
     3361 cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc tcttcttcca
     3421 tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct gaaaaacaat
     3481 atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat ccgtctgttc
     3541 tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc gggacacaat
     3601 cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac aagaaagtcg
     3661 tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat tactttaata
     3721 cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa gtaacgcctg
     3781 ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag aatgatacgt
     3841 tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa tctttgaatc
     3901 agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc aatattgggt
     3961 tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa caataccaag
     4021 aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg aataatccat
     4081 tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca gagctcaaac
     4141 agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt cagctggtct
     4201 tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg gttaacgaga
     4261 aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc aaaaagatca
     4321 aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat caagcgggta
     4381 ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg gctgcagatc
     4441 aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat catcttgacg
     4501 atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa ttaaaataaa
     4561 gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg aataaaagat
     4621 aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac aatatgaata
     4681 tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg atttaacttc
     4741 tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag aatatgccaa
     4801 accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca aacatggtgc
     4861 tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt ttatggtaac
     4921 aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt gggggcaagt
     4981 catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat
     5041 tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt atgaaataaa
     5101 ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg
     5161 tcctggagat atggtcactc catatattga atacaaacga atgaatggca gtaaatattt
     5221 actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag tattagagaa
     5281 tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc aagaagaagt
     5341 aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg cttttgcttt
     5401 agatactggt acagcaggag acactccagg aatgacacca aaagaagcag attcgatttt
     5461 aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata aaaaattatt
     5521 gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata cagtgatttc
     5581 tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc cgtctgtagc
     5641 cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg aagatgatta
     5701 tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg aaacagttga
     5761 aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata tcggaaaaaa
     5821 gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt cttatctata
     5881 atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc ggaagaaagc
     5941 atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc attcaactat
     6001 gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag taacctttta
     6061 ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt gatcaattca
     6121 gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat cagcggagtg
     6181 agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt catgccagaa
     6241 gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt ttcagcactc
     6301 atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt cgactatcct
     6361 gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga tagaattggt
     6421 acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc aatcgctttg
     6481 ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga attttatgaa
     6541 caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt aagcagttta
     6601 ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg tacgatgaaa
     6661 ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac aacaggttat
     6721 ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac gctgatcaaa
     6781 ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca ttatcatcat
     6841 gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc taagagaaat
     6901 ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt tgaagaattg
     6961 acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt aaaaattggt
     7021 ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc catgcgtatc
     7081 gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta tttggaaagt
     7141 ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct ttttgaagga
     7201 aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc gaaatcagct
     7261 aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga acaattgaaa
     7321 aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc agttgacaaa
     7381 gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc acgacccaag
     7441 aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg aacctatttt
     7501 gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata cgcttgaaga
     7561 aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct taagtggagg
     7621 cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc ctgatgatgg
     7681 ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac ttcatgaatt
     7741 gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt
     7801 ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa caattatgtt
     7861 cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc
     7921 aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc ttccagaaga
     7981 aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt
     8041 agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga aaaaaatcaa
     8101 tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt ctccagattt
     8161 caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt tgaatgtctc
     8221 agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag gagatatctc
     8281 tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat caaaaatagt
     8341 cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat tgcttacctt
     8401 actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta ttttttatac
     8461 aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag tatctttttc
     8521 ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta agtcaaaacg
     8581 ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc
     8641 actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt
     8701 tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta aagcgtggat
     8761 caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat ctgcttggaa
     8821 ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga taagatttgt
     8881 cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt ctaatatctt
     8941 ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca gattctccat
     9001 ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc gaagctgctc
     9061 ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc tccgatcttt
     9121 gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta gaatgataat
     9181 atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg tttggatgta
     9241 aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta cataaaaacg
     9301 gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc ttataacact
     9361 agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag ttgcataaag
     9421 cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc attttttata
     9481 gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga taataaataa
     9541 atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa tatcttctga
     9601 tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg agtattcaag
     9661 acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag cgataaaaac
     9721 agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac taatcaatgg
     9781 tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat aaggagatga
     9841 gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca cagccaatga
     9901 gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct tttcgcctag
     9961 atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt ccaacggttt
    10021 ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt
    10081 gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc acgaatcttt
    10141 tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga aataagcaat
    10201 tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac gtaaataaaa
    10261 agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc tttctaaaac
    10321 ttgtaatgtt agggtaa
//
LOCUS       DAZKNO010000093        10336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000093 DAZKNO010000000
VERSION     DAZKNO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.094"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..1527
                     /gene="arcD"
                     /locus_tag="ACG8MY_001772"
     CDS             106..1527
                     /gene="arcD"
                     /locus_tag="ACG8MY_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0430958.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFAIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVAFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            1541..2659
                     /locus_tag="ACG8MY_001773"
     CDS             1541..2659
                     /locus_tag="ACG8MY_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0430959.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(2719..4011)
                     /locus_tag="ACG8MY_001774"
     CDS             complement(2719..4011)
                     /locus_tag="ACG8MY_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0430960.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLAHQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNAAALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLMGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            4161..4721
                     /locus_tag="ACG8MY_001775"
     CDS             4161..4721
                     /locus_tag="ACG8MY_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0430961.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLEGIHETNQIILQIFEAFQLTKEEKVHRSRELCSMIHGYLSLRFLG
                     YFTKESTIDPEDSYFWMINDFIATLP"
     gene            4850..5227
                     /locus_tag="ACG8MY_001776"
     CDS             4850..5227
                     /locus_tag="ACG8MY_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0430962.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            5160..7232
                     /locus_tag="ACG8MY_001777"
     CDS             5160..7232
                     /locus_tag="ACG8MY_001777"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0430963.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKEMRINPDF
                     TIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(7443..8390)
                     /locus_tag="ACG8MY_001778"
     CDS             complement(7443..8390)
                     /locus_tag="ACG8MY_001778"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0430964.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDVDSVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            8635..9099
                     /locus_tag="ACG8MY_001779"
     CDS             8635..9099
                     /locus_tag="ACG8MY_001779"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0430965.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(9161..10204)
                     /locus_tag="ACG8MY_001780"
     CDS             complement(9161..10204)
                     /locus_tag="ACG8MY_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0430966.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     RATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         3443 a         1937 c         2155 g         2801 t
ORIGIN      
        1 caataatatg aataaagttg ctttttgtta agcaagagca gttgcaaaat agaaggaaat
       61 cttttgcaag tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatca
      121 acgggtatta gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt
      181 gtgtttggga tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca
      241 tggataatcg ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa
      301 aaacgtccag atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta
      361 ggtggcttca tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt
      421 gcaacgatgc tgatgagcgc catgggagaa tttttcgcga tattcaaagg tggacaaaat
      481 gttccttcta ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat
      541 ggagttgaag gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta
      601 ttcctatttt taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt
      661 tggggtaacg tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg
      721 cagattaaag gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt
      781 gttttagcca accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg
      841 ctttgtttat tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa
      901 gaagagttag caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga
      961 aattggggag cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg
     1021 tcttggacga tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg
     1081 aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca
     1141 atcttgacta atctattctt attgactttc ttagttactg attatgccta tcaatttgcc
     1201 tattctttat gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc
     1261 cagttttctt taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagct
     1321 tttgaattga tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc
     1381 tatcttcctg gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg
     1441 ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg
     1501 cttgctgtag gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg
     1561 tgtagaagaa tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc
     1621 gatcgaagca ttgacattag acgaactcgt tggcttagat gggactagcg taagtgaatt
     1681 tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt
     1741 caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa
     1801 tggagcgact ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt
     1861 tgtttcgatg cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc
     1921 tgttgacttt gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga
     1981 agccttagtc aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg
     2041 cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc
     2101 atatgtattg atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat
     2161 cgtagatgtc tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc
     2221 aggtattcgt attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta
     2281 tcgagactac acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt
     2341 gaagaataaa gaaaaaatat tggagcgaaa caaaaagatc attacagaga accttgtgat
     2401 tttaaaacaa tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc
     2461 tacttctttc attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt
     2521 agaagaaaca ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg
     2581 actaggttac tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt
     2641 cttgaaaaaa caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc
     2701 agtgtttttc atttacgctt attgtctatg agaaggagag tgccctgaag atatggagac
     2761 tcctgaagct ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac
     2821 aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga aagacgccac gataaccaaa
     2881 tgcactagaa acactggagc cgatcatagg tcctactacg ttaccagttg cttggaaaga
     2941 ttggttataa ctgaagatcc gtccagcagc ttgatgagga gaatattttg tgatcaatgc
     3001 ttggacagca gggagcagac aagcatcaga gatacccatc aaaaaacgta aggcagctaa
     3061 ttgccaaaca ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc
     3121 aatcgcaagt attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc
     3181 tatcaaagtg gcaatcccag gtatcgaagc gatgattccg ctgatcaacg taacattccc
     3241 atgtccatgc agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga
     3301 tgcttggatg atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc
     3361 tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg
     3421 gacgaaaaac agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt
     3481 agggcgatag ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc
     3541 tgtaacagaa ccagttgcta aagttccaag aacttgacca cttttttctt ttggcgtccc
     3601 agttgcgaca agggcagcgg cattactgat ataaccagaa aaaatccctt gaagcagacg
     3661 caatgcgact aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat
     3721 tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt tggtgggcta attttcccca
     3781 ccaaggagag atcagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa
     3841 gttcaattgt gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat
     3901 gactaaacta aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg
     3961 ttcccaagtt tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc
     4021 ttctttctga aaattttcat ctacagcttt ttgttataaa ctacaatatg tagtttatca
     4081 atgaaagtct cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt
     4141 ttatgagagg aaacatgaaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact
     4201 gctttcgaga actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcaa
     4261 aacgcttgga tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca
     4321 aaacagcgct aacagcgaaa cttctgcagg aacttaatga ccagctgcgt cgggctcttg
     4381 tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt
     4441 tttcaaatca agcagtgtat gagttactga tcagtgtgcc ccacaccaat gaagcagttt
     4501 tattagaggg aatccatgaa acgaaccaga tcattttgca gatctttgag gcttttcaat
     4561 taacgaaaga agaaaaagtc caccgaagca gagaattatg tagtatgatc cacggctatc
     4621 tttccttgcg ttttttagga tactttacaa aagaatcgac aatcgatcca gaggacagct
     4681 atttttggat gatcaatgac tttatcgcta ctttgcctta aacaatcaga ttcatttata
     4741 tcattttcaa gatgttcatt aaatgatcct tttataagag aaaattcttg caactttctt
     4801 gaaaagtgac taagataagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt
     4861 aattaccatg ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat
     4921 tgaagcagaa gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg
     4981 gaacgaaatc catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt
     5041 ttggaatgac ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa
     5101 aggacttgcg ttgttgaaaa atcatcagcc catcacagta gaaagcggaa aggaatttta
     5161 tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg
     5221 gtgctaatca aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc
     5281 ttggtcattt attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc
     5341 aaatggacac gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag
     5401 gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca
     5461 ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc
     5521 atttcaataa accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa
     5581 ctggattcaa gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg
     5641 cccgggcaaa agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt
     5701 acgcggataa tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc
     5761 aagaaaagaa aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg
     5821 gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag
     5881 cacaaaaact tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa
     5941 aattaaaaac ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca
     6001 atcaacgcta tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga
     6061 cccataagat cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa
     6121 gtacgatgaa agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt
     6181 tgaaaaacgg agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc
     6241 accatgcact tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc
     6301 aaaaaatcta tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag
     6361 tcagccaaac aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc
     6421 gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg
     6481 aaaaagaaat gagaatcaat cccgatttca ccatcaaaaa agaactatct gcaccgccta
     6541 aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag
     6601 ctacgcgtgc agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc
     6661 aaggactgca agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag
     6721 taacaccaga tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga
     6781 aacaagcagg aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga
     6841 tcaaatcaag caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat
     6901 gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt
     6961 cagattgcaa ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat
     7021 gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg
     7081 atggaacgac agaaaaaatg cttgataaaa aagagcagaa gaagaaaatg accaaacatg
     7141 aagaacgacg cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag
     7201 cagcagcact aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag
     7261 tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa
     7321 tttcggctta tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg
     7381 tgacaagact tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta
     7441 tattattggt gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa
     7501 acaggacgct gtttcgtttc taagaagatt ttcccgatat atttcccgat gattccaaga
     7561 cataacaatt ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat
     7621 gtaggatcac caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg
     7681 acacaagaca aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg
     7741 ataccatcaa tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca
     7801 cgttcgcggt tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct
     7861 tttgaaaatc ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc
     7921 aagcggaaat cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg
     7981 tagaattttt tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg
     8041 gtgccgacac tatcgacatc agaagtagtg atccggttga tcatctcagg aagcagttcg
     8101 ggcggatctt gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat
     8161 ccagcgtaca gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa
     8221 ggataagctg aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca
     8281 ataaacaaat attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga
     8341 ggaacagttt tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc
     8401 ctttcaatcg tatgtttata tatggtttac attcaaaatt taataaacct taaataacac
     8461 cttttaaatt tgattttatg gaaaagaaca gctattcttt ggtaaactaa cgtatttagt
     8521 ttaccatgtt tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag
     8581 ttttggatag agaaaaagga ggaagttcca gtctactgac caggagcaaa caaaatgaaa
     8641 atacacacac ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta
     8701 tcttttcagc caatttcaga aagcgaatca gcgaatcagc aaattttaac gcgcacacaa
     8761 gaacagtttg aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata
     8821 aagagaggta cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga
     8881 gaaatccgaa cgtataaaga agtagctgtt gctgttgata gtccgaaagc tgtgcgagcg
     8941 attgggcagg caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc
     9001 gggcaaaatg gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa
     9061 cgccaattgt tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat
     9121 tgttccatgc gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa
     9181 taaatagtaa agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc
     9241 gtgtaccttc tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag
     9301 tgatctcaaa ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa
     9361 acaagctgtt gaccaaacaa aagatcacaa accctaatac ataccaagga agtggtaatt
     9421 ttcgtttttt ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga
     9481 atttttcaaa cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca
     9541 tggcgaattc gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag
     9601 attggagtgt gccacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta
     9661 atccaaggaa agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga
     9721 tctgtccttt ttcttcctct ttcgcatgga tggaaggggc gatcgaagca atagcagaag
     9781 aaccacaaac agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt
     9841 ttttaccaat caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa
     9901 ttccttttat accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa
     9961 cagagaattc taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt
    10021 taaaaaaagt atttcctaat aaaataccca ataaaatagc aatagtcgct ctacctaact
    10081 gtggcagata aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac
    10141 cgggtaagat ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt
    10201 tcatttgatc ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact
    10261 aaaccagtat tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtca cgacctcttg
    10321 tttcgaataa acggtg
//
LOCUS       DAZKNO010000094        10305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000094 DAZKNO010000000
VERSION     DAZKNO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.095"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..304
                     /locus_tag="ACG8MY_001781"
     CDS             5..304
                     /locus_tag="ACG8MY_001781"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0430967.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            377..1738
                     /locus_tag="ACG8MY_001782"
     CDS             377..1738
                     /locus_tag="ACG8MY_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0430968.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1760..2020
                     /locus_tag="ACG8MY_001783"
     CDS             1760..2020
                     /locus_tag="ACG8MY_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430969.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2034..2786
                     /locus_tag="ACG8MY_001784"
     CDS             2034..2786
                     /locus_tag="ACG8MY_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0430970.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2880..3485)
                     /locus_tag="ACG8MY_001785"
     CDS             complement(2880..3485)
                     /locus_tag="ACG8MY_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0430971.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3538..4512)
                     /locus_tag="ACG8MY_001786"
     CDS             complement(3538..4512)
                     /locus_tag="ACG8MY_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0430972.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4890..5609)
                     /locus_tag="ACG8MY_001787"
     CDS             complement(4890..5609)
                     /locus_tag="ACG8MY_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0430973.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5736..7007)
                     /gene="celB"
                     /locus_tag="ACG8MY_001788"
     CDS             complement(5736..7007)
                     /gene="celB"
                     /locus_tag="ACG8MY_001788"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0430974.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7160..8557
                     /locus_tag="ACG8MY_001789"
     CDS             7160..8557
                     /locus_tag="ACG8MY_001789"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0430975.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8638..9078
                     /locus_tag="ACG8MY_001790"
     CDS             8638..9078
                     /locus_tag="ACG8MY_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0430976.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9226..9552
                     /locus_tag="ACG8MY_001791"
     CDS             9226..9552
                     /locus_tag="ACG8MY_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0430977.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9553..10302
                     /locus_tag="ACG8MY_001792"
     CDS             9553..10302
                     /locus_tag="ACG8MY_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0430978.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3266 a         1820 c         2139 g         3080 t
ORIGIN      
        1 aaaaatgaaa aatcggttaa ttgtcgcatg tggaagtgga gtagcaacga gtcagacaat
       61 cgctagtaag atccaaagta tgttagaaga tgacggcatc gcatttcctg tagaagcagt
      121 tgattacaaa tcgattcaaa atgagcttct aactgctgga atctatgtgt atgtcgcaca
      181 acctgatgag gaagtacttg aacaagcaaa agatttgggt attgaagtat ttccaggaat
      241 tccattttta actggaatgg gtgtagaacc tatttatgac agtatcaaag agttagtaca
      301 gtaaaggagg attgttggat aaaaaagttg gttcatagtt ttatttacta tatttagaaa
      361 gaaagaaggt ttatttatgg aagttttcca aaccattatc aattatgttt tgaacttagg
      421 atcagccatt tttgttcccc tgattatttt gatacttgga ttgttggcag ggatgaaatt
      481 aaaaaaagca ttcatgtctg ctttgacttt agggattgct tttagcggaa tgtctatggt
      541 cattggctat atgtctaacg cagtaagtcc agcatcagaa gcattggcta aaaatacggg
      601 aatcagtttg cctgcacttg atcttgggtg gacaggtgca gcaagcatca catggtcgtg
      661 gagttatgct tttatatttt ttgcagtaac gattggggtc aattttgtac tgttactttt
      721 taattggacg aagacgttaa atgttgatat gtggaatgtt tgggggaaag ctttaactgc
      781 ttatttagtt tattatgttt ccggtagttt ggctgcaggc tttcttactg caatggtcca
      841 agttatattg gaattaaagc ttggtgatat gtttcaaaaa catatacaag atctcacagg
      901 gattccatta gtaacagtga ctcattttat gacatctgcc gctgtcttgt tgctgccttt
      961 caatatgatc atggataaaa ttcctgcttt aaataaacga gcggatacga atgcattgaa
     1021 gaagaaaatt gggatcttct ctgaaaactc tgtaatggga ttcattattg gattggggct
     1081 tggctttgct gctgcgtatg gcgtttcagg ttctcttaat ctagcgatcc aaacagctac
     1141 agcattaagt ttgtttccta tgatctctaa aatgttcatg caatctttgt ctcctctagc
     1201 agacgctatg tcagaattta tgaaaaagcg gtttaaggat cgtgaagtat atatcggatt
     1261 ggactggccg atattagctg gtcgttcaga aatttgggtt actgctattt tacttgttcc
     1321 tgtgtttatt ggttatgcca ttattttgcc aggaaaccaa gtattgccat tagcagggat
     1381 tatcaactat tcgattgcgg tcggcggatt acttttaaca ggaggaaact tggctcgtat
     1441 gctgatcttg ggaatcatca ctatgcctat ttatttgtac ggggccactt accttgcgcc
     1501 aatactcagt gatttagcag aaaaaagcgg agcagtcgag ggaatcaaga aaggacagtt
     1561 gatcacatgg tcatccatag aagggccgga gttcagggtt ttatttgcgg aggcatttaa
     1621 taggaatatt ctcgctattg taggatgcgt gatttttcta ggactgtttg ttctacttta
     1681 taaatatatg ataaaagcga aagtaccgag tgaacgttat gctgaactac aaaaatagca
     1741 ttgaaatggt gggaaagtaa tgaaaagaga agagacagat aaaataaaat ggacagtcgc
     1801 tctttgtggt acacttctgc tatttctata tggactcttt actcagaata tcatcatcaa
     1861 tctattggtc atttttttcg cactagtgat ctataaatat ggaaatcacg ttttattcag
     1921 agagtacgat gaaaaaagaa aacggaaaat agaagaatca atgaaaataa aagaagctac
     1981 caaagaaata ttgagagaaa aaagttttat aaaaagataa tggaggggtc attatgtcag
     2041 atggacgtat gttatttgag agagaacgac aggacatggc acgtatcgta agagtgattt
     2101 tcgaaaggaa aaacacaaat gtcgcaggag ggaatttttc ctttaaaacc actgatgatc
     2161 aagggaaaga ctacatcatc atgacgccga ccatgatgtc agaagcctat ctaggagaga
     2221 tttctcctgc acaggttcta gtagtagaac caagaacgcg tagaatcgtt gacggagtcg
     2281 gtcatttaac tcgtgaaatc aatatgcatg aagcgatgta tgagactaat ccagctatca
     2341 aagctgtcct acatgctcat gcgcctaatg caatgttctg ggcgactact gggttgccta
     2401 tgcctaattt gacagaagcc acagctaaag taaaagagat tcctgtcatg gactttgagc
     2461 ctaattgctc tgaagaactt gctgaattga caagcgacta tgtaaaaaaa cagcatctcg
     2521 aattaccgca tatgttgctg ctcaactctc atggcgtatt gatcaacaca ggtggagaat
     2581 caggaatcga agctatccat aaagcactgc aaatccttga tacagttgaa tggaatgcag
     2641 aaattgccta caagcagacg atttttcaag cattagggat tactgacggt ttttattcaa
     2701 aaggtgagaa aatcggggga ttggaagagt tgaaacataa aacggctatt tataataaat
     2761 ctgttattgc agctggtgga gactaaccta tctgacacaa aaaactacga ctttcacaaa
     2821 atctgatcca gatcgatatc tgcttcaggt tgtattatgt aagtcgtagt tcttttcttt
     2881 tatttagtta agaggtggtc tgtccattcc ctcgcttgct tgatttcgtc catcgtcaac
     2941 tgatgacctg ttacagttag ctgaatagtc acatttgctt ggcgtgtttc gaattgatcg
     3001 gccaattctt taaaggaagc aggggaaacg attggatcgt tatcaccata agaaatccag
     3061 acggttttat cctctaatgt ggaacggctt tgatgaacac ctagagacat cggatggaaa
     3121 aacaaccctt tatttagctc ggtctcacgc tccaataata aatgagcagc gatgtttgcg
     3181 ccatttgaat agccgacaag cagccagtca cttaacggta tcagttcttt ttcgctgatt
     3241 tctttgattt tttcatggag tttatctgtt tctttttcca aactgtccag gtcgaattga
     3301 ttcaatccat tacgcttgaa aaaacgattc atcccatctt cttggaccgt gccacgaaaa
     3361 gaaagcaatg tactgtttgg tgcaagaaag tgagcgatat ccagtaaaga atgttcatcg
     3421 ccacctgtgc catgaagtaa gatcaaatgc ttgccacttg ttcctttttc aaaaatgtag
     3481 gtcattttgt tccctctttt ctgtttctat agatgcaaat gaagtagcat gaagagatta
     3541 ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg acataagctt caatggcttc
     3601 acgacgttct tctaagaatg gaggaagaga tagtttttct cctgctgatt ctgctggttc
     3661 atcttcaagg aatccaggac catcagttgc taattcaaac agtacgtgat gagcggctag
     3721 gaagtactca gattggaaat agaaacgatc gacgaaacca gaatttggca attcaatctt
     3781 attgattact tgaatccagt attccaatgc ttctttgtct gctacacgca atgctaagtg
     3841 atggatattt ccaaatcctt cgattgcttt tggcaagtct gtccgatgtt caataatgat
     3901 gcttgcacca tttccccctt caccgatttc aaataagtag aaatctcctg aatggtctgt
     3961 ttctttgaat cctaagattt tttcaagaat caagcggata tgctctaatt gactgacacg
     4021 gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc ggtacattgc tgtttttcca
     4081 aggaatgcca gctgctacac cttgattgtt ttcgtcagag atcaattgat aatactgatt
     4141 atcgaaatct tcaaattcga tatatttttt gccgaaacgt tcattgattg ctgaatgagt
     4201 aacgtcatat ttttcaaaac gttccaacca aaagtctaat gctgcgtcac ttggcacacg
     4261 gaaagaagca cgagagatcg tatctgttcc tttttttcct tttggctgat ttggaaaatc
     4321 aaagaaagtc atatctgttc ctggcgcacc agtatcgtct gtaaagtaaa gatggtaagt
     4381 ttcgatgtca tcttggttaa ctgttttttt gattaagcgc attcctaaaa tatctgtaaa
     4441 aaagcggtag atttttgtag cgctagatgt cattgctgtg acgtggtgaa ttcctctgat
     4501 ttgtgtatcc atgataaatt tcctcctgtt aacttacaaa aagtaagtag ttgatttaga
     4561 catagtatag agggtgcatc tctattttgc aattaatttg tcttctaaag tatcaggaag
     4621 gaatcaagaa tggaaaagag gagggacgga tcaataaaat aaaaagactt acgactagct
     4681 gtatcgattt caaaaaaact agctttaaaa agctaacttt agaatcatta cgggtggtca
     4741 taagtctttt tatgctgagt tattttttca tttttgagct aatgcttagg atagaataga
     4801 atgttgattt cacgatctct tcttgttact tgaaactgct taccactgtc acgatctctt
     4861 ggtaaacggc tttttcacaa cctctttggt taagttgaac gcacatccag cacagaatag
     4921 ttccgaacat caaatcggtt tcggctggaa gaatattcaa tcggctgtcc atctttgaga
     4981 tagacgactt gctgaacttc cagtacagga tcgttttctg cacagttcaa atattgctga
     5041 tcatattcat cacttttatc tgcagaaata gcacgataag cccctgcaaa atggacattc
     5101 aattcttgat gaagatgatc atagatagag gctaacaaat gttctttttt taaacccggc
     5161 acaagatgga ctggcataaa ggtatgctct aaaacatatg gctcgccatc taagatcctt
     5221 aaccggtgaa tattgtagac aggttgctcg gcatcgatca tcaatcgttc ttggatatat
     5281 tgattaggga attcaacatc aaaatgaatg atttgactct tcaaagaacg atgtctgcgt
     5341 ttcatttgga gactcaaacc ttcatactcg ttagcgggcg atgtgtcttt ttgcataaat
     5401 ggatgattca agattttcgt tcctgatcct cgctgcgaat agacaagtcc ttccattgct
     5461 aagatattga tggctttttt gattgtcatc cggctcgcac caaattcttt taccaagtcg
     5521 acttgattag gcaaaaaact atcgagagga tactcttttg aacggatacg ttctcttaaa
     5581 atatttgcga tttcttcgta ttttggcacg ttattcacct cttctagagt ataacgaaag
     5641 attatgcttc cgacaactcg aagtagcatc tggaacattg tttattcgaa aaaagaggtc
     5701 atgacaagtt tttgtcacaa ccccttaatg atctattaag caatatctaa atctggttgt
     5761 ttatctagta ctttcaggaa tgggtaccag ataaaggcaa cgatgacaaa gtcgatgact
     5821 tgcagcatac caccaagcca tgaatttgtt gctaagacac cgcttgcaat gattggcaca
     5881 gtccatggaa cagagacgcc agttggtgca gggacaagtc cagcagccat taccaaataa
     5941 ttgaacgtcg ttacgattag aggtgccaca acccaaggaa taaggattgt tgcattcaat
     6001 acgatcggta gaccgaagat cgctggttca tttacattga aaataccagg tgctaaagct
     6061 aatcgtccaa catcacgata ctgtttcttt ttaagaacaa aggccatcaa taaaacgaca
     6121 gctaaagtca ttccagagcc accaatacca acagtgaagg tttccatgaa tggttttgtg
     6181 atgatgtgtg gtaattcttt tccatgttga taagcttcta agttatcaag catcagtgta
     6241 ttccaaatcg ggtccattac agagttgacg atgatttgtc cgtgaagtcc gaagaaccat
     6301 aagaattgga caaagaacaa agcaatcaat gttgcaggta aactacttcc caaaccagtt
     6361 aatggttttt ggatcacttc ataaacgacg tcatgtaagt tagcattgaa aataccagtc
     6421 atcacagcat tgattactaa gaaaatagtt agtgtcacta cagcagggat caaagcggca
     6481 aatgattttg ttactgcagg cggaactcca tcaggcattt tgatctgcca gccgcgtttc
     6541 gtgattctgc agtagatttc tccagccaag aaagcagcga tcattccaat gaacattcct
     6601 tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat cttcaccgcc agcggttttc
     6661 attgcaaaag gcgtaagaat caagaagctg gcaaaagaca cggcaccacc gaaaatacct
     6721 tcaacgtcat aagattttga taaataatag ccgataccaa aagtaacaaa aaccgacatg
     6781 atgctcattg ttgcattttg cccattaccg aataaattcg ataaagttcc ctttgttgcg
     6841 tcactgaaaa aaggcaagtt attgataacg acaacgatcg aaccgaacat cgttaaaggg
     6901 aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac gattttgccc taataaatta
     6961 gctaagggca tgattttctc agaaagcttg tccataaaac cgttcatttt tattcacctc
     7021 aaatttattt gcaatcgatt acatacaaag tataacgcca aaaaatttaa atgtctagtc
     7081 attttcataa aaagtattga tttatttttt agggttcgtt atgattacgg tatcaagaaa
     7141 aaagagagga agtaaaaaaa tgaaaaccta cacattccca gaaaattttt ggtggggttc
     7201 tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat gatggtaaag gagaaaatat
     7261 ttgggatcat tggtatgaat tagagcctga aaaattcttc gatcaagtag gacctgaaaa
     7321 aacttcgcaa gtttacacaa aatacaaaga agatgttcag ttaatgaaag aaactggcca
     7381 taacacattt cgtacatcta tccaatggag tcatttgatt ccagaaggga caggtgaaat
     7441 caatcaaaaa gctgtttctt tttatcgaga ttattttact gaattgctgg ataatggtat
     7501 tgaacctttt gtgaaccttt accatttcga tatgccacta ccactgcaag aaaaaggcgg
     7561 atggttatca agagaaacgg tcgacgctta tgtttcttat gcagcagcgt gctttgaact
     7621 gtttggagat ctggtcaaaa aatggtttac tcataatgaa ccaatcgtac ctgtagaagg
     7681 aggctacttg tatcaattcc actatccaaa tcatatcaat atgaaagaag cggtccaagt
     7741 aggctatcat gagaatctag caagcgcaaa ggtaatcaaa gcctaccatg aaggcggaca
     7801 tgatggtgag atcggaatca tcttgaatct gacaccaagc tatccgagag atgaaacgaa
     7861 cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc ttcaaccgat cattccttga
     7921 ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa attgtcaaag aattggctat
     7981 aatgccagag atccatgaag gagacctaga actcatccgt gaaaacacga ttgacctgct
     8041 aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa gaaactccaa tcaatcacga
     8101 acatagcccc atgccagatg attattttga taattacgac atgcccaata aaaaaatgaa
     8161 tccatatcgt ggatgggaaa tttacgaaaa aggaatctat gatattttaa cgaatacaag
     8221 agaaaactat ggtaatatca aatgctatat ttctgaaaat gggatggggg tcgaaggaga
     8281 agaacgattt gttaacagcg aaggaattat tgaagatgat taccggatcg agtttgttac
     8341 ggatcatttg aaatacgtcc atcaagcaat ccaagaagga accaattgtg tgggttacca
     8401 catgtggaca tgtatggaca actggtcttg gacaaatgct tataagaatc gctatggctt
     8461 tatcgctgtt gatttggcac aagacggtaa acggacgatc aaaaaatcag gtcgctggtt
     8521 taaaacggtc tctgaaaaaa acggatttga tgcctgatat tttttgataa aacgttcaca
     8581 aaatgtttgt tctaacactg taatcgtgct acactaacag tgaaggaggc gtttacaatg
     8641 gtagatactt atcgtaatat tttagtagca attgatggtt ctgaaaaggc agaaaaagca
     8701 tttcttgagg caatcacgat tgccaaacga aatcaggcaa cgttgcacat cctgtatgtc
     8761 aatgaagtaa caggcaatta ctttggtgat tttgcatttg ttacaacaag ccttcaggaa
     8821 gaattggatg aggttgcgga aaaccagatg aaagaacatc gaaacttggc aattgaaaaa
     8881 ggcctgacag atatcgagac atatgttctt tacggttatc caaagacact gattgctaat
     8941 ttccatgaat cgaaagaacc aatcgatctg attgtcatgg gggcgacagg tctgaatgcg
     9001 gtagaacgtg cactagtagg ttccactact tcctatgtag tcaaccacgc atcatgtaac
     9061 gtcatggttg taaaataaat aaattaaggg aacagaaagc aaaaaagcat aacgcttctt
     9121 ttgctttctg ttccttttat ttttttgtaa aaaattactt tacctgatga agtaatcatg
     9181 ctattctaat tacaacgaca gatggagtaa tatggaggaa agaaaatgat ttcaagtgat
     9241 ggaatacgcg gatacaatga tgcgattatt ttatcgatcc ttaacaaaaa cgattcttat
     9301 ggatatgaaa tttctaaaac gattcgtgag aggacaaatg attcctatgt gatcaaagag
     9361 acaacacttt actccgtttt tgcccgttta gaaaagaatc aactgattgt ttcgtatcct
     9421 ggagaagtga ctcatggaaa aaagcggaca tattataaaa tcactgaaat agggcgtaat
     9481 cttttagaag aaaaaaagca agagtggctg acgacgaaag acgtagtcga tcaatttttg
     9541 gagggaaatt aaatggatac aatccgtaat tatttagaca gtctttttat cggagtacct
     9601 caaagtaccg agatagataa gctgaaaact gatttgttgg caaatatgga agaccattat
     9661 catgaattga tgggagaagg aaaaaatgaa caggaggcca tcggtacagt aatttcgaca
     9721 tttggttcaa tcgatgaatt attagaagaa ctagatgtcg aaaaaaaaca tcaagctgac
     9781 gagacggaaa caaacacagc ttctatttat ttaagcgaag cagaaaacta ctggaaagaa
     9841 tacagagcgg caagtctcca agtggcatca ggtgttctat tcattagctt atcttttgca
     9901 agttttcttt tcttttgttc agcaggatat gtgttcatgg gtatttcttg tctgattttc
     9961 ggtatagcat tagctgtcgg tttttttatt gcttctggta tgaaaatcac acgtttaaat
    10021 cactttttgc accatcggaa aatacctgaa aaagtattag cagaagcaaa agaaaaagaa
    10081 gaagagtatc agcgaagttt tggcttctca ctgattgcag gaattggatt atgtattttt
    10141 tcacttttcc cacttttggc ttctttaatg tggtatatgg atggtagtat cggtgcctct
    10201 atattttttg ttacggtagg aacgggtgtc ttcttgatca tttatggcag tctagtccgt
    10261 cattcttatc gtcaattcac tcaaagcgct tattactggt gaatg
//
LOCUS       DAZKNO010000095        10238 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000095 DAZKNO010000000
VERSION     DAZKNO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.096"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            160..390
                     /locus_tag="ACG8MY_001793"
     CDS             160..390
                     /locus_tag="ACG8MY_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0430979.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            487..1788
                     /gene="murA"
                     /locus_tag="ACG8MY_001794"
     CDS             487..1788
                     /gene="murA"
                     /locus_tag="ACG8MY_001794"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0430980.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1801..1977
                     /locus_tag="ACG8MY_001795"
     CDS             1801..1977
                     /locus_tag="ACG8MY_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0430981.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2112..2408
                     /gene="yidD"
                     /locus_tag="ACG8MY_001796"
     CDS             2112..2408
                     /gene="yidD"
                     /locus_tag="ACG8MY_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0430982.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2592..3059
                     /locus_tag="ACG8MY_001797"
     CDS             2592..3059
                     /locus_tag="ACG8MY_001797"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0430983.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3144..4028
                     /locus_tag="ACG8MY_001798"
     CDS             3144..4028
                     /locus_tag="ACG8MY_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0430984.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4142..4378
                     /locus_tag="ACG8MY_001799"
     CDS             4142..4378
                     /locus_tag="ACG8MY_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0430985.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4466..4546
                     /locus_tag="ACG8MY_001800"
     tRNA            4466..4546
                     /locus_tag="ACG8MY_001800"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4500..4502,aa:Tyr,seq:gta)
     gene            4553..4624
                     /locus_tag="ACG8MY_001801"
     tRNA            4553..4624
                     /locus_tag="ACG8MY_001801"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4585..4587,aa:Gln,seq:ttg)
     gene            4749..4919
                     /locus_tag="ACG8MY_001802"
     CDS             4749..4919
                     /locus_tag="ACG8MY_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430986.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5139..5846
                     /locus_tag="ACG8MY_001803"
     CDS             5139..5846
                     /locus_tag="ACG8MY_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0430987.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5906..6550)
                     /locus_tag="ACG8MY_001804"
     CDS             complement(5906..6550)
                     /locus_tag="ACG8MY_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0430988.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6677..6964
                     /locus_tag="ACG8MY_001805"
     CDS             6677..6964
                     /locus_tag="ACG8MY_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0430989.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7072..7392
                     /locus_tag="ACG8MY_001806"
     CDS             7072..7392
                     /locus_tag="ACG8MY_001806"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0430990.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7473..8786
                     /locus_tag="ACG8MY_001807"
     CDS             7473..8786
                     /locus_tag="ACG8MY_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0430991.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8783..9502
                     /locus_tag="ACG8MY_001808"
     CDS             8783..9502
                     /locus_tag="ACG8MY_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0430992.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9630..10181
                     /gene="raiA"
                     /locus_tag="ACG8MY_001809"
     CDS             9630..10181
                     /gene="raiA"
                     /locus_tag="ACG8MY_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0430993.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3415 a         1693 c         2187 g         2943 t
ORIGIN      
        1 ttgtctcagt cccttttttt gcatattttt agcatttatt tcctaaaaaa tgtaagggga
       61 ttcctatatt tttaagtata cttgtttttc cttttataca ttttatggta ttatcatttt
      121 attagttatt ataaaacaat cgttagaaag gacgtcatta tgcaagtata cggaattgat
      181 gcggtgcttc gaatcgtgag tcatttggcg tttatctact tagccttctg gtctttacgt
      241 tcgttacgca ttgaaacttt tttcagacca atgcataata cgcaaattag aatggcaatc
      301 gtactttttt caattttttt agggtacact gcaagtaatt ttttcttaga agttatcgct
      361 ttatgccgaa atcttttcgt tagcctgtaa attttaaaaa aaggtactat tttaaaagca
      421 aggatgttat aatagaaaag attttgagta agtgtatgct cattggaatt ttttggaggg
      481 aacaacatgg aagagatcat cgtaagaggt ggcaatcaat taaatggaac agtaagaatt
      541 gaaggagcta aaaatgctgt tttgcctatt ttagctgcta gtttgttagc agaagaaggt
      601 attacgacat tagacaacgt tccgattctt tctgatgtct tcactatgaa tcaagtgatt
      661 cgtcacttaa acgtagatgt agattttgat gaacaaaaga atcaagtaac aatcgatgct
      721 tcaagacaat tagaaatcga agcgccttat gagtatgtta gtcaaatgcg tgcttcaatc
      781 gttgtgatgg gtccgctact tgccagaaat ggacatgcaa aagtagctat gcctggtgga
      841 tgtgcgattg gtaaacgacc aatcgatttg catttaaaag gatttcaagc tttaggagca
      901 aaaattattc aaaaaaacgg ctatatcgaa gcaattgcgg atgaattgat cggtaacacg
      961 atttatttag atttccctag tgtaggtgct actcaaaata tcatgatggc tgccgtaaaa
     1021 gcaaaaggaa caaccatcat tgaaaacgta gctcgcgaac cagaaatcgt tgatttagca
     1081 aacattttga ataaaatggg cgctcaagtc tatggagctg gtactgaaac aatgcgtatt
     1141 gaaggcgtgg atcatttaca tgcagtcaac cactcaatcg tacaagatcg tatcgaagca
     1201 ggtaccttta tggttgctgc tgcaatgaca caaggcaatg tgctgattgc agatgctatc
     1261 tctgaacata accgcccatt gatttctaaa ttgatcgaaa tgggtgcaga aatcattgaa
     1321 gaagaaggcg gagtccgtgt catcggacca aaacatatct tacctacaga tgtaaaaacg
     1381 atgcctcatc ctggtttccc tacagatatg caagctcaaa tgacagctat ccaattagtt
     1441 gcagaaggta caagtgtagt gactgaaaca gtgtttgaaa atcgtttcca acatttagaa
     1501 gagatgcgcc gtatgaacgc tcatgtcaaa atcgacggaa atgtagcaat catggatgga
     1561 aaccatgaat tgcaaggtgc agaagtatat gcaacagact tgcgggcagc agcagcatta
     1621 gttttagctg gcttgaaagc aaatggaatc acacgtgtgc gtaacttgaa ttatctagac
     1681 cgtggatatt ataatttcca tatcaaacta cagcaattag gtgcagacgt tgaacgtgta
     1741 gacatggatc aaacttctgc tgaaaaaaca gcacaaacga ttgcttaagg aagtggctga
     1801 atgagtaacc aacgatatat catattaact ttgataaaga ttttagtggt gatcttattg
     1861 cttatccttc tttttgtggc cggaacgatg atcggatatg gtgtcatcgg tggcggcaat
     1921 ccatttaaag tattccagcc aagtttgtgg attcatattc gagacttttt tcattgataa
     1981 ataagaatca cagtggttct agtcgatatg aagtgagcca tgaagcagaa catgacaaaa
     2041 gaaaacagaa gggaacgttg acgttagtcg cgatcccttt ttttagtaaa agaaaagaag
     2101 gaatctaatt gatgaagaag atattgattt atttagttag aggatatcag cgctttattt
     2161 cgcctgcatt tccacctagc tgccgttatt acccaacctg ttccaattat atgataaaag
     2221 caattcaaac ccatggtgca ctaaaaggga cggtaatggg gactgcacgg atcttacgct
     2281 gtcatccttt tgtcaaaggc ggtatcgatt atgtaccatt gacattccaa ttaacgcgaa
     2341 ataagaaaga taaaaaagaa ccagattata aaaaggttgt gaaaaatacg agtagcactg
     2401 agcgataaag aaacaatttg gaaaatagct tcttatattt tgcaaaattg tgagttattg
     2461 ccgaagtgtt ggattttgaa caccgtcaaa aaaaggttgt gaaaaatacg ggtagcactg
     2521 agcgataaag aaaaagagga aaattattgt caaagtaaca actccgcaac tacttatatg
     2581 ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc ttgttattag
     2641 atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt ggctggatat
     2701 ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa aaagaatacg
     2761 aaatcgtcaa tgtagcagta gccccaacgt atcaaggaaa ggggattggc ggaaaattac
     2821 ttgagacagc ttttcagaag ctttcacaac tcgcttccag tcaaactcag atcatcatac
     2881 gaaccggcag tactagcagt gcggcgttac atctctatca aaaaatgggc tatgtggaga
     2941 ttggtcgagt caaagactat tttatccatc attactctga gcccattttt gaaaatggag
     3001 atcaattaag ggatcaagta attttagcaa ttcacttacc taaaaatagc tttaactaat
     3061 aaactttcaa ttcgagaaaa tgctttcaaa tgctaaatag atacattaca atagctttag
     3121 caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt ggatcggctg
     3181 tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg
     3241 aatcttggga agaaatgaaa gcgcaattat ttcaagttta taaaaaaata ctcgtttctt
     3301 ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag aaacaggtga
     3361 ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga gatttggaaa
     3421 ctctgtttca actcccagta acgattgaaa atgacgccaa ctgtgccgga atggctgaat
     3481 tttatgaagg tgctgcccgt gattatcaag atgttgcttt tgttgtggca ggaactggga
     3541 ttggcggggc gttgttcacg aaaggacaaa tcaataaagg ggcacatctt tatggtggag
     3601 aatttgggtt gatgttctta aatggcgata agacattcag taaacttggt acagcggtac
     3661 agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaattcttat accggaaaag
     3721 atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt gagagttttt
     3781 atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca gaagtgatcg
     3841 tattaggcgg cggtgtttct gcaaaagaag ggctcattga tgagatcaaa agccgaatga
     3901 agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta ttatgtgaat
     3961 acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta cgtcaagggg
     4021 agaattaaga gctttagaga aggtgcagct tgcataaaag aagtgaatga aatgaatagg
     4081 agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct gagaggtgaa
     4141 aatgatgtca aaaaaaaata aagatgttga agtgcgtata gaagaagtga aaaaagatat
     4201 caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga ttggtgagat
     4261 cttagcctat gggccaaaag aatatgaaat tttctttggt gaagaagatt ttggaaaaga
     4321 aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc acgaataaaa
     4381 aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag gtattataac
     4441 taagtcaggt tgatacaaac ctttcggagg agtagcgaag tggctaaacg cgacggactg
     4501 taaatccgtt ccttagggtt cagtggttcg aatccactct cctccatttc attggggtat
     4561 agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc
     4621 ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tctttttttt gctttgctac
     4681 cttgttcctt gaagattctg ctgatttaag atagagttaa gttaactaca agaattggag
     4741 ggatccacat gaaatggaaa gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg
     4801 tcaaatcaga aaaatacaat attgaagtga atattccgaa aaaaggcgga aaagcggtcc
     4861 aggtaaaatc aaaacgccca acgaatcata caaaaaaatg gatggctaaa aaccgctaat
     4921 aataacaaaa gatccataag tcggcatttt catcgatttt agggtctttt gttattttct
     4981 aggttgaaca tatctaaaaa attgtttctg acaataggag taggaagaac gatgtgtgtc
     5041 taaatcctct gttgatggat agaaaccttt atctttttca gttgtgaatc aggtatatac
     5101 cagttataat agaagaaacg aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat
     5161 atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag
     5221 accgtcttcc ttcagaaaga gaattagcag tgaaattcaa tgtcagccgt atgacgttga
     5281 gacaagcaat tcaaaacttg gcagatgaag gaatcttaga aagaaagatc ggttcgggta
     5341 catatgttgc gcgagaaaaa gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa
     5401 ttatggaatc gcaaaatcga gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc
     5461 caagctcaag tgaaatggaa aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg
     5521 aacgtattcg tttcgcagat gatgtcccta tttgttttga agttgccagt gtgccacaaa
     5581 agctgatcga tggctacagc aaagcagaga ttactgaatc gttgtatcgc acgctagaag
     5641 aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat tactgctgtg ttggcttctg
     5701 agaaaattgc tgactattta gaggtaaaac gaggagatgc agttcttcgt ttgcgccaga
     5761 tttcttattt tgaagacggt acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc
     5821 gttttgagtt ttatttggaa aaataaaaaa caggtgatcc ctgattcctt acttagaaat
     5881 aaaagagaat cagaatcacc tgtttttata tatgatcagt taccagttgc tcgtgataag
     5941 aaacaggacc ctcttcaaat tggacttgtc cgttcagcag ctcaatgatg gattctttaa
     6001 agtggtcagc acttttttca tctacagcac agatgacgct aacttgatca gtatattgga
     6061 tttcttttat aaaaaaatgt tggagttcaa gataattctg taattttcct agttgcggat
     6121 acgtgatcat cacagagatc tcctgttgca aggtaccttc tacgattcca atgtcagaca
     6181 atgcttgaga gacagcatga gtgtatgctc gtattaatcc gcctgtgccg agtttcgttc
     6241 ctccaaaata tcgagtgact actgcagcta cattgatcag ttgatttttt ttgaggactt
     6301 ccaacatcgg tacgccagcc gttccacttg gttctccatc atcgctgctt cgctggattt
     6361 catttttttc tccaatgatg aaggcgctgc agttatggtt cgctttccaa tgttcttttt
     6421 ttatttcttg aatgaaactt tttgcttggt cctctgattc tattcgtcta agagaacaga
     6481 taaagcgtga ttttttgatc tcaatctcac tttgtccatt ttcttttatt gtacgatagc
     6541 gctcgatcat cttgtcacct ctttttttca ttttctttat tcagtataaa taaagttcta
     6601 ggttgtttca acactgtaac ggttgcgcaa tcgctgtagt atgcgataat aaagacaaag
     6661 gtaggtggat ctggggatgc aggaacgaat gaaaaagtac gatgcaatta cgcattattt
     6721 gaagaacaat ggcggctctc aagtaacgtt gacctttacc caatttgacg aactgctgtt
     6781 tcccagtaac ggacttccca aaacagcaag ggaaagtaca gattggtggg caaatgatta
     6841 taaacaccct gaaaaaggtg cctatggctg gataaatgca ggctatgaag tagttgtcat
     6901 caatttggac aaagaatatg tcgtgttcaa taaattagtg aaatctagtt ggctatttga
     6961 ttaaataaac cgcgtttaga aaacgttgac aaatttcttt ctttttatat aatagagtaa
     7021 cagacgcgaa gaaaacgttt gcgtagtttg ttttaaaaag gagagaaaag tatggctaaa
     7081 aaaacaatca tgcttgtatg ttcggctggt atgagcacaa gtttactagt aacaaaaatg
     7141 caaaaagctg ctgaagcaaa aggaatcgac tcggatattt tcgctgtgtc cgcttctgac
     7201 gcagataaca atcttgcaaa taaagatgta gatgtactgt tattaggacc tcaagttcgc
     7261 tttatgaaag ctgattttga aaaacgtctg gaaccaaaag gcattccttt agacgtcatc
     7321 aatatggctg attatggtat gatgaatggc gaaaaagtgt tagatcaagc aatcacgctc
     7381 ataggtgaat aaatagaaca gtataactat cgaacaagcg atccaaaaaa tagatcgctt
     7441 gttttttcgt atattttttt agggcgaata atatgaatca tttttggggt agaaggcttc
     7501 tgacgagaga tattaaagca atagactgtg aggaggtaaa tttaccaaat tatgagaaga
     7561 tcgcagcagt ggaacgtttg ggcaactgga ttctttgtca tcgctgtgga atgaggacgt
     7621 ccgtcattga tggacagctt gcagattacg gttatttttg tacacattgt ctatatttag
     7681 ggcgatgcga cactcaacaa gatttatatt tgtttgatca gcctgaagca aaacctcgag
     7741 aagtcgtctt tttttggaca ggacagctta ctgaaaaaca aacggaaatc gcaaagagaa
     7801 tactttgcca ttcagaaaaa agacatcatc ttatttgggc tgtaacaggt gcagggaaaa
     7861 cagagatgct ttatcctatt ttagtggaaa ctttaaaagc tggaggaaga gtagcgattt
     7921 gtacaccgcg agtcgatgtt tgtaatgaat tgtttttacg ctatcgttat gtttttccag
     7981 aagaaaaaat cacgttgctc catgggaata cagcaacggc ttataccttt acttcttttg
     8041 ttatctgtac gattcatcag ctataccgtt tttatcggtc ctttgattta atagtgatcg
     8101 atgagatcga tgcgtttcct tataacgggg atgcaggatt agctcatgct gtgcaaacag
     8161 cgattaaacc agacggacga ttcatctact taagtgccac acctgataaa aagttactaa
     8221 aggaaatcaa gcagacgttt gacatccaca aacttgcttt gcgctttcat cggcgtttac
     8281 ttcctgaacc cgtcttggtt ttcatgaata attggcgaca aaaatgcaag aacaagaaga
     8341 agataaaatc tttggtgcgg atcattcatc aattaattaa agaaaaccat atccttttat
     8401 tttgtccaag tatcacgatg atgcatcgtt tgaaagccac acttcaagag gctcttccga
     8461 gatatgagat cacagatgtt tcttcaagag acaatcagcg ggcggaaaaa gtccagaaaa
     8521 tgcgagaagc aaaatatgac atactgttaa ccacgatgat ccttgaacgt ggagtcacat
     8581 ttgaaagaat atccgttatc gttttaggag cggatcatcc ggtttttact aaatcttctt
     8641 tggtacaaat cgcaggaaga gccgatcgta aagggcctta tacgaacagt caagtatact
     8701 ttttctatga agaaaaaact gcagctattc gaaaagcttg tcaggaaatc aaagaaatga
     8761 atgaagaagg gaagaagatg ctgtgaggtg cagttgctgc agtaaagata tcgtccaaaa
     8821 tttaacattc agcgagatta ttggtgtttc gaaatacaga ttacctcaga cttgttcagt
     8881 ttgtcgcgca caatttcatt tattggaaac aagaggctgt tctggctgtc aaaaaccaat
     8941 ccatttcaat ctagagagta cgaaaaagaa agtaaagtct attcccgttt actgcagtga
     9001 ctgtcaagaa tggaagaaga aatatccgac ctatgatttt cagcaccgtg ccttttttgt
     9061 gtacgatgaa aatatgaagg cctggctcaa tcaatacaag tttatgggag atatccgact
     9121 agcaggcacc tttgcatata aatggacgca gataaaaaaa gaatacccga catacatcta
     9181 ttgtccaata cctttatctg aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga
     9241 gatgttgaaa gcagcaagaa ttccttttca gatgctactc aaaagagaga agcatctgct
     9301 ggctcaagca aaaaacacgc gaaaggaaag attggagatg cctcaacctt ttgaacttgc
     9361 agtcaccttt tctaagatac aaaataaaaa aatattgatt attgatgatg tctatacaac
     9421 aggacagact cttttccacg cagcagattg tttgcaggtc gcacagccga aaatcatccg
     9481 aaccatttct ttagcaagat gaattagatg tggaaatttg atagcgattt aatttgcaaa
     9541 taacctagag ttcgatataa tagaattaag aagagggaaa gagtggtcct tctccttctt
     9601 gattgtggta gacgaaaggg gtaattttta tgtttagata taatgtacgc ggcgaaaata
     9661 tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct
     9721 atttcagtga cgcacccgat gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa
     9781 cagcaaaagt ggaagtaacg atcccattac cttatcttgt attacgtgcc gaagaaactt
     9841 ctccagattt atacgcaagt atcgatttgg ttgttgacaa attggaacgc caaatccgca
     9901 aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg catgaatact gcagatgcta
     9961 ctgctttatt caatgaagag gacgaaaacg aaaatgattc tgaattagat atcgtacgta
    10021 cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc agtcttacaa atgaacatgc
    10081 tgggacataa cttctttatt tttgaagatg cagaaacaaa tggtacaagt atcgtttacc
    10141 gccgtaaaga tggaaaatac ggactgattg aaacagactg atcagcctta cttaaataac
    10201 aacgaaaact tgcagcagct actgacagct gccgcaag
//
LOCUS       DAZKNO010000096        10179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000096 DAZKNO010000000
VERSION     DAZKNO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.097"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..963
                     /locus_tag="ACG8MY_001810"
     CDS             130..963
                     /locus_tag="ACG8MY_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0430994.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            979..1716
                     /locus_tag="ACG8MY_001811"
     CDS             979..1716
                     /locus_tag="ACG8MY_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0430995.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(1871..2839)
                     /locus_tag="ACG8MY_001812"
     CDS             complement(1871..2839)
                     /locus_tag="ACG8MY_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0430996.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            3071..3904
                     /locus_tag="ACG8MY_001813"
     CDS             3071..3904
                     /locus_tag="ACG8MY_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0430997.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            3938..4777
                     /locus_tag="ACG8MY_001814"
     CDS             3938..4777
                     /locus_tag="ACG8MY_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0430998.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            4803..5363
                     /locus_tag="ACG8MY_001815"
     CDS             4803..5363
                     /locus_tag="ACG8MY_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0430999.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            5562..7283
                     /locus_tag="ACG8MY_001816"
     CDS             5562..7283
                     /locus_tag="ACG8MY_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0431000.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            7448..8590
                     /locus_tag="ACG8MY_001817"
     CDS             7448..8590
                     /locus_tag="ACG8MY_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0431001.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            8606..9817
                     /gene="thiI"
                     /locus_tag="ACG8MY_001818"
     CDS             8606..9817
                     /gene="thiI"
                     /locus_tag="ACG8MY_001818"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0431002.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         3479 a         1715 c         2136 g         2849 t
ORIGIN      
        1 agtccattcc ctgtttttta tggtccctgt gctataattc taaataaagg ataaggattg
       61 gaaataatcc ttttatcatt aaaaatatga tgacgaagta gaataaaatt gggagtaggg
      121 gggaaagtca tggatatcca aactgtttta tctgatctga aattaaatgg taaagtgatt
      181 ccagttgttg gtggcgatgt taatcagacc tatcgtataa aaaccgaaca tcgtgcttat
      241 tttttaaaaa tccatccgaa tgtaaaaaaa ggattttttg aagcagaagt agacggatta
      301 aaagaactgt cagcattcgt acgtgtaccg gatacgtata tgctaggtga aacaagtgaa
      361 ggcgcttatt tgttaatgga atggatcgag ccgggaaaag gggatcaacg agatcttgct
      421 gcagctttag ctaatctcca tcagcaaaca gcgccacaat ttggttttcg taaagataac
      481 tatttgggta cacttgttca gaaaaattct tttgaagaag attggtggac ttttttcttt
      541 aaagatcggc ttgaatccca gatttctttg gcagaagaaa cgaatcgatg gaatgtacag
      601 cgtcaagaaa agtatctgcg atttaaagaa cgtgtattga aatcggttga accaaaaaag
      661 atcactccac gcctcttgca cggagacctg tggagtggta atgtgttctt tgaccagcaa
      721 gggcatccag tttttgtcga cccagctgtt tcttatggga atcgggaaca agatattgca
      781 atgagccagt tattcggtgg tttccgaccg gaatttttag atgcttatca aactatattt
      841 ccattagaaa agggatggaa agaccgactg ccgatttacc agttatatta tcttttggca
      901 catttgaata tgtttggaga aagttacggt tcccaagtag atcaattact agaaaatttt
      961 taagaggagg gcgtgttttt ggcaaaaaaa tataccaaaa agcatgaagt ttcatattat
     1021 gagtgtgaca tcaaccaaac gatgacattt ccttcgatgt taggagtagt tatcaaaacg
     1081 tctgaagatc aaagcgacca gttgggaaga ggcagtgaat tcgtcaatag ctttggactt
     1141 acttgggtca tcacgaatta ttccatggag attaatcgtc tccctagagt cggtgaagta
     1201 atctcagtaa cgactcaagc catggagtac aataagtttt tctgttatcg gaacttctgg
     1261 atccatgatg aagatgggaa agaattagtc aaaatagaat ctgttttcgt attgatggat
     1321 tttgtgaatc gaaaaatgag cagtgttaat gaagagatca ttgctccttt tgagagtgag
     1381 aagatcaaga aaatcaagcg tcaggaaaaa attgaaaaga ttgattcggg cttcatgttg
     1441 ccatatcgtg ttcgttttta tgatatcgat agtaatcagc atgttaataa tgcgatgtat
     1501 tttaattgga tcattgacgt attaggatat gactttttga ctacgcatgt tccagaaaaa
     1561 gtaattattc gatttgataa agaagtagag tacgggaatg agatcgaaag ccattttgaa
     1621 caaatcccta tcgaaaatgg tgtcaaaaca aaacatgaaa tccgaatgca agaacagctg
     1681 tattgtgaag caaatattat atggaaaaat aaataaaaaa gaggttgtga cagaaggggg
     1741 caacttcaag aaataagaag aaatttacga aaattgtttc tcaaattttt gtaaattttc
     1801 gacttagttt tcgaagaagc tatttttgtc acagcctctc tttttaagtt tagctattag
     1861 ataatgatgt ttagttttgg ataaacgaaa tcaattcttc acccgacaaa ccatagttta
     1921 cagcaatcaa tagttttaaa cgtgcttttt gactattaat actgttacag aaaataatcc
     1981 ccattttttc tagttctttt cccccgcctt cataatcata aacaggctct gcgattccat
     2041 tgaaacaacg agaaacaagt actacaggga ttttttggac aagaagcttt tccaaagcag
     2101 ctaaggcttg tggagggaga ttccccgcac ctaaagcttc gattaccaaa ccgtctactt
     2161 tcgtatgggc gatggcttcc aataagtccc cttgcatccc agcatatgct ttaacgatcg
     2221 ggatagtccc atctacagcg gaaatgtcca gtcgctttgt ttctaaaagt tcctgcaaga
     2281 ataaaattcg attcttagtt actaagccga tgggacccaa tgtaggtgtt cggaaagtag
     2341 caacatttgt ggtatgggtc tttgtcacgt agcgtgcaga atggatctca tcgttcatga
     2401 ccaccaaaac ccccttatct agcgcttctt cacaacttgc gacacgaatc gcactttcaa
     2461 aattataaag gccgtcactt ccaagttcat tacttgaacg cattgcccca gtcaaaacaa
     2521 ttggtaattt gttgccaatc gttgtatcaa gaaaaaaagc agtttcttct aatgtatctg
     2581 taccgtgtgt gatcaccact ccggatatac ctgatgcata agcggacaag atacgttctt
     2641 ttaataccaa catgtgagaa ggcatgatgt gtggtgaggg aagattgaag atatcttcaa
     2701 ctaataattc tacatgcttg gggattgtga tttctgcatc taataatggg tttgccacat
     2761 caggactaac accgcccgtg agcatgtttt ctttcatggc gatcgttccg ccggtgtgta
     2821 gtacaagtat ttttttcatt ttacagactt ccttacgaat aattttgcat agccgttaaa
     2881 tcttattgta tactatataa aagagttttg ctatcagtga agaataacga atagcctaac
     2941 ttatcataaa aaagctgacc ttattaaaat ggaaaagact caaccttgac ctcctaatac
     3001 aatggagtat ctggttgagt ttcttttaca agaaagcagc caaggaaatc aatcacgaag
     3061 gtggccggaa atgatcagaa tcggattaac atcgtttaaa gaacatgaca aattaactgg
     3121 taaaaagaat aacactcttt atgaatatgc agggtttcta cccttagtgg aattagatac
     3181 tgcctattat ggaattcccc gaaagtcctc agttgagaat tggataagtg aagtacctga
     3241 atcatttcgc tttgtagtca aagtctatca aggaatcagc ggtcaaggag actggaagcc
     3301 ttattatcag accgaagaag agatgattgc gcatttttta gatgttatgt cgccaatggt
     3361 agatgctgga aagatgtttt gctttttagt ccaattcccc tcatcattca aatgtacgaa
     3421 agaaaatgtc cagtacttgc gcaaacttag aaagtggttt gagggttatc ctgtttctat
     3481 agaattgcgg gattattctt ggtacagcga tgaatttctt gaacgtacaa gaaactttat
     3541 gtctagagcc gctttttctt tagccatcat cgatgagcca cagttgctga atacaacgat
     3601 tccttttgat ccatttgtca ccaatccgaa ctttgctctt tttcgattcc atggtaggaa
     3661 tgcgcaagga tggcaagcaa atgataaaga gtggcgtaaa aaaagaacgt tatatcgtta
     3721 ttcagaaaaa gaactgcaag aactagcaca tgaagtcaaa gtagttgatt ctaagacaaa
     3781 agaggtaggt gtgattttta ataataattc tggtggtgat gcggctgaaa atgcattagc
     3841 actgcaaaaa atattaaata tcaagccaga tggactcaat ccaacccaaa tggatctgtt
     3901 ttaagggctt attttattca agaaaggggc gaagtctatg atcaaagcta ttttttttga
     3961 tattgacgga acattggtca ccaatcgttc aaaagcgttg gaatcaacaa aacaagcaat
     4021 tgaatatgct cggaaaaacg gcattttatg cggtgtagca acggggcgca gcccagtaaa
     4081 gatcaaagaa atcattgatg aattggaatt agatatgtat gtcgtgtata atggtcaact
     4141 ggtttttact gcagatcgca cgattatcga ccaccctttt gaacaaaaag tattggaaca
     4201 tattgttgaa tttgcagatg agaaccatcg acaaatcgtt tttggtgcga gaaatcggtt
     4261 agatggatca acgacgatgc tattaggaca atcgattttt atcaagcgac tagtcagctt
     4321 tttaccgcgt aaattcccgg tacgtctgat gaaaaaaatc ctccaggttt ttagccctca
     4381 ccgacaaaag gatcgttatg aaacgttaga gatattaaaa gaaccaattt atcaatgtat
     4441 attgctcagt ccggagtcag aacagaaaaa gttagaacat cgctttcccg agtgtacgtt
     4501 tcagcgttca aatacgtata ctgtagatat catcccaaaa ggtggttcaa aattactagg
     4561 catccaagct ttcgcaaatg ctgtcgaaat tgaaatggaa gaaattatgg cattcggcga
     4621 tcattacaat gatatcgaga tgctgaaagg tgtaggaatt ggagtggcca tggggaatgc
     4681 tcagatcgaa gtaaaacatg ctgcagactt tgtgactcaa tcaaatgaag aagatgggat
     4741 atacgaagga ctgaaacact tcggtctgat ttattagtca atgaaagaag gaaaaaatca
     4801 gtatggaagc agagacacct tttcaaaaag tagaaacatt ccacaaaact tttgatccaa
     4861 gaagaccaga gatacccacg gcttttacag aaaatgaagc tcatacacgt gctgcgttca
     4921 aaatagaaga acttgttgaa tttgttgcag ctacttcacc taataaagaa caactgcatg
     4981 aagctgttat gctgctgcac gaagcattag ataaagcaga agaaaaaata cttggtaaaa
     5041 cacagtgggg aagtgatccg ctggttgaac aagtagatgc actgacagat atcctttact
     5101 ttacttatgg ttctttttca ttactaggaa tagtgcctga tcgaatattc gaaatcgtcc
     5161 atcaggcaaa tatggggaaa ttatttccag atggaaaacc tcattatgac cctgtaacaa
     5221 ataaagtgtt gaaacctgaa aactggcaga tggaatatgc tccagaacct aaaatcgcgg
     5281 cagaaatcca acgtcagatt caagaagcga ttgctaagaa gaaatccgct gatataggca
     5341 tgtgcacaaa tgaaaaagag tgatcagagt ttcactattt ttgttatctt tatacgatgg
     5401 aaaaacaaaa gattttcgtt taagttagtg aatgcatgaa aatagtatgc tataatctag
     5461 aatagatgtc ttttgcacga gagacgtatt atttggattt atgccaagca gaacgccatc
     5521 tgttgcataa aagatgcata ctgaaaacgg aggaaccata aatgggaaat aatgttatca
     5581 tcggaatcgt cattgcaatc attatagtcg ccattgtcgt ctttttggtt agttacttga
     5641 tgaagaaaaa aaaccaagat cgattggatg ccttagaaaa acgcaaggaa gaattatttg
     5701 acttgccagt gattgaagaa gtcgatgatg ttaaaaaaat gcatctggtt gggcaaagcc
     5761 aaaatacctt tcgtgaatgg agtcaaaaat ggaatgacat atctacttcc tcgttcgctg
     5821 aactagaaag tcaaattttc gaagtggaaa acctcaatga aagttttcgg ttcttaaaag
     5881 gaaaaaaagc agttgttgaa gcagaagcta caatggacca aatggagaaa caagtgacgg
     5941 aaatccgtga aggtctaaag gaattgcggg aaacggaaga acgcaactca ctagctgttc
     6001 agcaggcact tgacgtctat gaagaactga aagaaaaagt cagagaagac gagcatgaat
     6061 acggaccagc tctttttgaa ttgcaaaaac agatcaaagt gatcgaaaaa gaatttacgc
     6121 agtttgtgac tttaaacact tctggagacc ctgttgaagc ccgcgaggtt ttggataaag
     6181 cagaaaaaca tacgtatgaa gtggaagctc tgatgaaaaa aattccaccg ctgtatgagg
     6241 agctccatac cactttccct gaacaacttg aagaaatcag tgatacttac gagaaattga
     6301 tggaagaaca atatgtattc ccagaagaag atcttgctga agatatcgca aaagtaagtc
     6361 gaagaattga aaattcatta gccaatcttg aaaaaacaga agttgaaacg gtcgaatttg
     6421 aaaaccgtga aacagctgat ttgattgatt cgttatatga tatcttagaa cgggaaatgg
     6481 aagctcagag atatgtaaaa actaaccaat caacgattgc agagtatatc aaacatacaa
     6541 caaagaataa ccgtcaatta ttgattgagc tggatcacac atctcaaagc tatacattga
     6601 accacaatga aattggccgt gtacgaggtt tccaaaccga agtggaagaa atggaacgtc
     6661 aaaatgaaca gatgattcca cagatccgtc aacacgaaat tccttattca gaagtgcgca
     6721 cgttctataa aacagtcttc aaagtcttag aagatatcga aaagcaacaa gtagagatcg
     6781 acgactcatt acatgaattg agaaaaggcg aaaaagaagc gcaagaaaag atcgatacat
     6841 tcgaattcaa attgcgcagt ttgaaacgtt ttgtagaaaa acaacgtttg cctggcttgc
     6901 cgaatgatta cctagaattc ttttttgttg ctactgaccg tattgaagaa ctaagtgttg
     6961 tcttgaataa aattcgagta aacatggaag aggtcaatcg tttagttgct ctatgtgaag
     7021 aagatctaga attattagat aagaagacac atgatttagt agatgcagca gcattaacgg
     7081 agcaaatgct ccaatatgca aatcgttatc gtcatacaca cgaagaagtg agagaagcaa
     7141 ttgataaaag ctactattta ttcaataaag aatatcacta tcaagaagca ttagacgaaa
     7201 tcggtacagc attagagcga gtagaacctg gtgctttcaa acgcatcgaa gatttttatt
     7261 ttaatcatcc agacttagta taaagcaaaa aagttagtaa accagcaaga gcaagacgga
     7321 ttattgtctt gttcttgtct ttttttgtat atttgaaaat tgaaacaagt gaaaatttca
     7381 cctgctcttg aaagcacctt ggacaaccgt tataatagaa aagaatttca aaaaagaggg
     7441 gtttcttatg atatattttg ataacagtgc aacaacccca atctttccgc aaagcttaga
     7501 tgcatatgta aaaaccagtc agcggattat tgggaatcca tcaagcttgc atgatttagg
     7561 caatcaagct aaccggttgt tgcagcaagc aagaaagcag atcgctgatt tgatccatgt
     7621 agaaccagaa gaaatctatt ttacttcagg cggaacagaa ggagataact gggtattaaa
     7681 aggaacgatg atcgaaaaaa gagaatatgg gaatcatatg atcatttcag gagtcgaaca
     7741 tccggccgtc agcgagacag cagaacaatt aaaagaatta ggaatcgaag taagtatcgc
     7801 cccagtggat aaacgcggat ttgtacgtgt cgacgaattg aaagaattaa taagaaaaga
     7861 gacagtatta gtctctgtta tggctgtgaa taatgaagta ggatcgattc agcccatcca
     7921 ggaaatcagt gatcttttgg atgattttcc taaaattcat ttccatgtag atgctgttca
     7981 ggcgattgga aaagtaccat tctcgcaatg gttgacagaa agagttgatt ttgcaacatt
     8041 ttcagctcat aaattccatg gcccacgggg aactggtttc atttattgga aaaagggtcg
     8101 tcgtttagca ccattactga caggtggagg gcaagaaaga aatcagcgaa gtgggacaga
     8161 aaatgttcca ggtattgtgg ctatggttaa agctttacgt ttatcattag agataaagga
     8221 acaaaaacct gagcagacac aacgcttaaa agtagctcta atcaatgcgt tgaagacgta
     8281 tgagaaagtg acgatttttt cagaaggaac tgactatgca ccgcatattt tatgttttgc
     8341 tctaaaagga attcgcggcg aagttctcgt ccatgcctta gaagaaaaac aaatatttgt
     8401 ttctactact agtgcctgct cctcacgtaa acatatggca agcagtacat tgcacgcaat
     8461 gcatgtacca aatgatctgg ctacatctgc tgttcgaatc agcttagatg aaaacaattc
     8521 gcttgcagaa gtcgagcaat ttatgattat tttcaagcag ctttataaga aattttctgc
     8581 aatcaattaa aagaggagtg ttcttgtgaa atacagtgaa attatggtcc gttatgggga
     8641 actttcgaca aaaggaaaga atcgaaaaag ctttatcatg caactagcac aaaatgtcag
     8701 acaagctttg aaagactttc cagaaatcaa aatccatgct gatcgggacc ggatgcatct
     8761 tttattgaat ggcgcagacg ggcagctagt tattccaaaa ctagcaaaaa tatttgggat
     8821 ccaaaatttc tctccaagta ttcgtgtaga aaaagatgtc gaagttttga aacaagctgt
     8881 tcaagacatc atgaaagaaa tttatactgg gaaagaaaca tttaaaatta tcgctaaaag
     8941 aagcgatcac caatttgaat tgacaagtaa tgaattgaat caaacattag gtaatgctgt
     9001 ttttgatatt ttcccgcata tccaagttca aatgcgtcaa ccagatattc cattacgtgt
     9061 agagattcgg cgtgatggcg cttatctatc gtatgaaacg atcaagggag ctggaggatt
     9121 gcccgtagga acaagcggaa aaggaatgct tatgctttct ggggggattg attctcctgt
     9181 agcagggtac tttgccatga agcggggagt ggaaattgaa gctgttcatt ttgctagtcc
     9241 gccgtataca agtgaacaag cactgcaaaa agcaaaagat ctaacagcca aactagcacc
     9301 ttatgtggga acgatccatt ttatcgaagt gccatttacc gagattcaag aagaaatcaa
     9361 aaagtcagtg ccacaaggat attggatgac aattactcgt cgaatgatgt tgcgtttgac
     9421 cgatgaaatt cgccgtattc gtcatggttt agtcattttg aatggtgaat cattaggtca
     9481 agtggcttca caaactttgc aaagtatggt ggctatcaat gaagtaacga atacgccaat
     9541 cattcggcct gtagcgacaa tggataaact tgaaatcatt gaagtagcag aacagatcga
     9601 tacatttaat ctttctatcc agccttttga agattgctgt acgatttttg ctcctccaca
     9661 gccaaaaaca cgccctcaac tggaaaaggt ccatcaatat gaagagcgtt tagcaataga
     9721 tgaaatgatc caacgagcac tagctggctt gaagatggaa gagatacaag cagaatcgtc
     9781 taatgaaaaa actgaagaat tttcagaatt gctataagag gttatgaaaa tagtgttttg
     9841 accagagtat tagagaaaca aaacgaaaaa atagctccat gtattttacc gtttttgaac
     9901 taatgtcgaa gattagctga ttgaatacca tttatcaaga agttatggaa aagccggttc
     9961 ttttttacta actgaaaaat aaaattttat ttagagatca agctacagtt gataaatgat
    10021 ataaaaaaga gaatttccca tgaaatagcg aggttgtgac agaagtgggc agcttcaaga
    10081 aataagaagg aatttacgaa aattgctttt caaatttttg tgaaatttcg gcttattttt
    10141 cgaagaagtt acttctggaa caccgtgtat cagaggttg
//
LOCUS       DAZKNO010000097        10097 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000097 DAZKNO010000000
VERSION     DAZKNO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.098"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            232..2067
                     /gene="tdc"
                     /locus_tag="ACG8MY_001819"
     CDS             232..2067
                     /gene="tdc"
                     /locus_tag="ACG8MY_001819"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0431003.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2372..3850
                     /locus_tag="ACG8MY_001820"
     CDS             2372..3850
                     /locus_tag="ACG8MY_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0431004.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3816..5147
                     /locus_tag="ACG8MY_001821"
     CDS             3816..5147
                     /locus_tag="ACG8MY_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0431005.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5261..7813
                     /locus_tag="ACG8MY_001822"
     CDS             5261..7813
                     /locus_tag="ACG8MY_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0431006.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7820..9259)
                     /locus_tag="ACG8MY_001823"
     CDS             complement(7820..9259)
                     /locus_tag="ACG8MY_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431007.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9234..9416
                     /locus_tag="ACG8MY_001824"
     CDS             9234..9416
                     /locus_tag="ACG8MY_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431008.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9605..9967
                     /locus_tag="ACG8MY_001825"
     CDS             9605..9967
                     /locus_tag="ACG8MY_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0431009.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         3213 a         1659 c         2141 g         3084 t
ORIGIN      
        1 cgcttttttt aggttatcta ttttagaaga ctgatttatt gaaaaattgt tcgctttttt
       61 gcgttgaatt atcagaaaaa tccgacttta tttatttttg tttgtaaaaa tctacgtgga
      121 tttgatgtta taaaaaaaaa attctcattt ttggcgctat agtgaagagg tgttacagat
      181 aaacacatgt atctgcaaga cattgaccaa caaaatatgg agggaagaaa aatgaaagat
      241 atggatatca aggccgtctt tataggcgac aaagctgaaa atggaccagt ttataaaatg
      301 ttgttgaaca agatggtaga tgaacatctt ggatggagag aaaattatat tccttcagat
      361 atgcctgcaa ttagcgaagg agataagctc actccagatt atctagccac ccgcgaccat
      421 atgatagagg tcttggatga agtcagtcag cgattacgcg caggatcgat cccatggcat
      481 tcagcaggcc gttactgggg acaaatgaat gctgaaacat tgatgcctgc tttattagct
      541 tacaactatg cgatgctgtg gaacccaaat aatgttgcat tggaatcttc tatggcaact
      601 tcgcaaatgg aagcagaagt aggacaggat tttgcttcat tattcaatat gacagacgga
      661 tggggacaca ttgcagcaga tggttctatt gctaatcttg aaggattatg gtacgcccgt
      721 tgcatcaaat cgattccttt agctgtcaag gaagtgctgc ctgaaaaagt gaaaaaaatg
      781 tctgagtggg aacttttgaa tttatctgtt gaagaaattt tggaaatgac cgaaagcttt
      841 acggacgaag aaatggatga ggtcaaagca gcttcttccc gtagcggaaa aaatatccaa
      901 agattaggga aatggctggt ccctcaaaca aaacactatt cttggatgaa ggcgttagat
      961 atttgcggag tcggcttaga tcagatggtt gctatcccag ttcaagagga ttatcgaatg
     1021 gatattaatg ctttggaaaa aacaattcgt gaattagctg gacaaaaaat cccaattcta
     1081 ggtgtggttg ctgtcgtagg aacgacagaa gaaggtcaag tagacagtgt agataaaatc
     1141 gtccaattaa gagaaaggtt aaaagatgaa gggatctatt tctatttaca tgttgatgcc
     1201 gcatatggcg gctatgcgcg ttcactgttt ctaaacgaag caggagaatt tgtgccgtat
     1261 gcttccttag ctgaattctt tgaagaacat catgttttcc accactgcgt aacgattgac
     1321 aaagaggtat atgaagggtt cagagctatt tcagaagcgg attctgtcac gatagatcct
     1381 cataagatgg gctatgtccc ttatgctgct ggaggaatcg taatcaaaca taaaaatatg
     1441 agaaatatca tttcttattt cgcgccatat gtgtttgaaa aatcagtaaa ggctccagat
     1501 atgttaggag cttatatttt ggaaggatca aaagctggag caaccgccgc cgcagtctgg
     1561 acagcacatc gtgtattgcc attgaatgtg accggatatg gtcaattgat tggggcttca
     1621 atcgaggcag ctcaaagatt cagagaattt ctcgatcatc tgacttttac tgttaaagga
     1681 aaaacaatcg aagtctatcc attgaatcat cctgatttta atatggtcaa ttgggtattc
     1741 aaagaacaag gctgtacaga tttgaacgct atcaatgaat taaatgaaaa aatgttcgat
     1801 cggtcttctt atatggatgg cgatgtttat ggtgaacggt ttattacttc tcatactaca
     1861 tttacacaag aagattacgg tgattctccg attcgttttg ttgaaagaat gggtctaacg
     1921 aaagaagaat ggaaaaaaga acagaaaatc actctgctac gtgcggctat catgactcct
     1981 tatttgaatg atgatcgaat tttcaatttt tatacaaaaa aaatcgccaa agcaatggaa
     2041 aagaaactaa acgaaatcat ccaatagaac tgatttttgt gtgaaagaat aacaagagac
     2101 atatggagaa cagacaatta tttgcttcat caaaacaatt gtatttcccc ctgtttttca
     2161 tatattcctc gccaagatat tcttttttat aaaacgttct ataagttgga tcgttcgttt
     2221 catgtatcaa acccagttca ctaagatcgt tcaatggatg tttcggtgat actgggtttt
     2281 gtactgaatc gatgaaacct tgtcatttct gttcttgaac aaaaataaaa gaaaacaaaa
     2341 acatatgtcg ctattgaaag aaggaataaa gatgaaagat actggttcat caaagcaaat
     2401 cacgaccatg acctttatcg gaatgacctg cgcccttgta gcaagtgtac gaaatattcc
     2461 agatgtagca gcaacgagct ggacgatgat tttttacatg ctggttgcag tattattgta
     2521 cgcttttcca atctcattga tctctggtga atttgctgga atgtttcccc aaaaaggtgg
     2581 tccagaactt tgggtatcga atgctctagg aaaaaaatgg gggttcgttg tctcatggct
     2641 gttatgggtc caaatgtttc ctgggatggt gatggtcgct tcagcactcg ctccgttgtt
     2701 tggtaatatt atcgataatg tgccgcttgg tttgaacagc aaatttacat tagttgtcat
     2761 tttagtcgtt tattggatca tcactttttt gaatttgaaa tttgatatgg caaaaatcgg
     2821 cggaaaagtc ggtgtttggt taggattgta cgtgcctttg acgatgatgc tcttattagg
     2881 gtttgctgcg tggatcaaag ttgggatcgt accaaccgga acattaggca gtttttcatg
     2941 ggacaaatta ataccagata ctacgactgc atcctcattt gtttattttg cgccgatcat
     3001 gtttattttt actggtatcg aaatgtcctc tgtgtatatc actcgattgg aaaatccagt
     3061 taaaacgtat attcgcggag tatttgccgc gttaattttt atatttttcg tcaatatttt
     3121 gaatgcttta gttgtagcaa atgtcgtacc aaaaggacaa atggaattga ataatatcgc
     3181 gcaatctatt tcgatttatt gccaaattct tggtttgcca catcttattg tgaatctgtt
     3241 tagtttactt gtcttcattg gtgtagcagt ccaattatct gcttgggcct caggaccagc
     3301 aaagacagtg acagaaagtg ctagaaaagg tgcgtatcca ccaaaattca atttttggaa
     3361 gacgaatcaa tttgacgtat ccaaatccgt tattttgact cagtctgtca ttatctcaat
     3421 ctttgcactt ttttatttgc tgattcctgg cgtcaatcaa gcatttttga tgctcgtaaa
     3481 ttcaactact gttatctact gtatcgtcta tgtcatcatg gggatcgcag ttttgagatt
     3541 gcgttataca catgcaaagt tgaatcgacc atttagaatt ggaaaaaaag agaaaaagag
     3601 caatcttgga gtttggactg tcgttattgt tttatttgcg gcaatcggat tttctgtcgg
     3661 actgacgatg aaagctggaa catggatcaa tctaattgca gtcacagcca tcagtgtcat
     3721 tttgtttgta gtaccactct gtattgaaaa aataaagaaa ccttcttggg aacaggaagt
     3781 cgccatgctg cttgaaaaag aaaaaggtga acttgatgga aaaaccggca atgaaacgga
     3841 ctctaactaa tcgaaatatc cagttaatcg cattaggtgg cgcaattgga acaggattat
     3901 ttttaggttc agcaaaggca attgcatcag cagggccagc tgttattctc gtttatttga
     3961 tatgcggttt ggctatttat ggtttgatgc gggccatggg tgatttattt ttagcaaata
     4021 gtacatttaa ctctatcagt gatttcgttg actattattt aggcaaaaag tgggcatttt
     4081 ttgtcaattg gacttattgg ctttgctggc ttggagctgg acttgcggaa ttaacagcaa
     4141 taggattgta tatccatttc tggtttgtac atgtaccagc tttgataagc ggaggtagcg
     4201 cactaatgat cttaatggca gtcaaccttg ttgcagtcaa gtggtttggt gaattggaat
     4261 cttttttctc aatcatcaag atcttgggga ttttcctttt tatcttattg ggtataggat
     4321 tgagtgtctc agattttcat acccagtcta tcggaatcaa agaaaatttt ggacagatcg
     4381 atgaattact accgtttggc tttgctggaa tggtagtctc atttcctatg gttttattca
     4441 gttttgctgg aattgaaatg atcggcttaa ccgttggcga gacaagtgat ccgcaaaaga
     4501 atttgaaaaa agcaatcaac tgtctgcctt ggcgtattct ctttttctat ataggaacga
     4561 ttttgtcgat tttatttgtt gttccatggc attgtttaga tttaaaagaa agtcctttag
     4621 tgactatgct gcaagtgatc cattgccaag gcggagcagc aattgtcaat ctaatgattt
     4681 tggttgcttc tttgtcctca gcaaatagtg cagtctacag tactagcagg attttatatc
     4741 aaacagcaaa agaaaaaagt ttgccccatt ggttttgtgc gctttctaag aaaggcgtac
     4801 ctttacacgg tattttaatt acaggaatgt tattttttgc cggtctttgt ctacattttt
     4861 ttgtagctga tagtcgattg ttttttcagc ttttggcagg catgacaacg agttgttttc
     4921 tatttgtgtg gtcttcgatc ttgtgtgcac atgttcaatt tattaaagaa aagaaacgga
     4981 acactacaca aagatataaa gatcttgctt tgttggtgtt ttttacttca attgctttca
     5041 gcttgttatt cgaaagcacg acacgcttta ttcctttttt attggttatc tggtttgtat
     5101 tcttaaattt tgtatataaa agaataagca atagaaatat cagttaggcc cagatacatt
     5161 ttgcaaaacg aattatttca gacccccctg aaataatcga tacgcctgaa aaaacttccc
     5221 tgcgtataat aacagtatca attaaacagg aggggatagg atgaactggt acagattgac
     5281 ggtagaagat gtattgaaaa actatcaaac atcaacagaa ggcttgtcgc aagaagaacg
     5341 acagaaacgc ctgactgatc aaggatataa tgaaataaaa gcaaggcagc agacaaaacg
     5401 atggaaaaaa atagcaaagc attttactga tctgctgatg atcgtgttga tcattgcctc
     5461 ttttttgaaa tttgcttcta gtgaatatat tgaaggaagt attattttat tcgtagtgat
     5521 cgtcaatgga ctcgttagtt actggcaaga aagaaaagcg gaagaatcat tgaatggttt
     5581 gaagcagctg atgggacaag aagcgatagt actatcaaac gggatgcaag aaaaaatccc
     5641 tgcgaaagaa ctggtaaaag gagatgtcat tgtcttacgt gccggagatg tcgttccagc
     5701 agatatccga ttaatcgaag cacatgattt attagtggaa gaatcgattt tgacgggaga
     5761 atcagaagca tctgaaaaaa gtcatgccgt tttgacagaa gaagaatcga tcggcgacca
     5821 gaaaaatatg ggtttttcag gaacgcttgt acaatctggt tctgcagtcg gtgtcgttgt
     5881 agaaacagga aacacgacag aaatcggtaa aatcaatcaa gcattgcaat cggtcgaaca
     5941 acaaacgacg cctctgatta aaaaaatcaa ccgattaaac aaacaaattt ttcaaggcat
     6001 tctttgtttg agcctattct tagttatttt tacgacattt cgatatggta tggattggca
     6061 tatccttctt tcggctacga ttgctttggt ggtgtcaatg gtacccgaag gattgccagc
     6121 tgttttgacc atgattctat ccgttggagt acatgaaatg gcaaaagaaa aggcgatcat
     6181 caaaggactt ccttcggtag aaacattagg ttctatgaca gtgatctgtt cagataagac
     6241 aggaacattg acaaaaaatg aaatgacagt tgtggatgta gctgcaaaag aagaagcctg
     6301 cgtgttgtcc attatgaaaa actgtcagga attaaaaaca aaagaagaac agaaaacaga
     6361 aaatttaagt ggcaatccga cagaagttgc gctgattcga cacacagaaa cagccaatct
     6421 acctttgaaa aaaatagaag caaagattcc gttcagttct gaatacaaat atatggcaac
     6481 gatgcatgcg gaggaaaaag gagcggtgat ttacgtcaaa ggtgcaccag aagtattatt
     6541 tgctaaatca actctttctt ctgcagaaca agaagcatgg agtcagacag ctgcagaatt
     6601 tgcgagaaaa ggtcagcgtg ttttaggatt tgcttataaa aaagtagatt cgaaacagga
     6661 actcacgcat gagactttaa cccaattaac ttttgcaggg atcgccggat tgatcgatcc
     6721 gccaaaagaa agtgcagtca aagctgtaaa ggaatgccaa caagcaggga tctcagtgaa
     6781 aatgattaca ggggatcaca aagacacagc aaaagcgatt gcggaacaaa ttggcttaaa
     6841 acatactgcc aaagttttag aagggatcga tttggatctt atgtctgatg aagagttgat
     6901 tcagcaagtg ccaatagtcg atgtctttgc tcgaacgaca ccagaacata aactgcgaat
     6961 tgttaaagct ttgcagaaaa atggagaaat cgtcggaatg accggagacg gcgtaaatga
     7021 tgcacctgct ttgaaaagat ccgatgtagg aattgctatg ggaatcaaag gtagtgaagt
     7081 aagcaaacag gcagctgata tggttttagg agatgataat ttccatacga ttgcaaaagc
     7141 cgtaaaagag ggccggcgga ttttggataa tttgcaaaaa accatcaatt tcttcttgcc
     7201 aactgcatta gctcaaggat tgatcttgat ttgggcactt atgctgaatc gcccgctacc
     7261 tttgtcccca gtacagattt tatgggtcaa tatggtaacg acaatcactt tatcttatgc
     7321 actagggttc gagtcgccag atccagaaat catgaagcgc ccaccaagag atccaaagca
     7381 aggaatccta tctggttatc atttgtttcg gatcatctat gtctccctac tgatcatggt
     7441 tcctgcctat ttattggcta tgcagtttga agggcaaagc ttacagcaaa ccattcttct
     7501 gcaaaatatc gtcttagctc aagctgttta catgatcaat tgcagagagt tgtccaagcc
     7561 gtcattgaac caaggtcttt tgcaaaacaa agcgcttttt ctttcattag gtatccttgc
     7621 tttattgcaa gggatggtta tttttctacc agtcggccag cagctgatcg gtacagtaag
     7681 tttaacgatg acacaacagt tattgattgg agcaaatgtg attcttctat tcttagttgt
     7741 agaaatagaa aaagggatca tgaacaaact gtttcgcaaa aaagaaaaaa cagctagaga
     7801 atacagtcgc tagccgttac tataaaaaag ttttgaaagt gagttcagtc aaacgattga
     7861 gctcattttt cgttggaata gaagaaatgt atacaatagg gatatctaga gaatctagag
     7921 ggaaattgga aatcaagata tctgtttttt caaaagaaat agtggaaaga tcggttagtt
     7981 cgataggtaa gaagcagaca cgttttccta agtaatcttt taattcctgc tgcaagaacg
     8041 ccttccatga aggttctcct tggaaaataa aatagacatt gaacaaattc ggtttgatag
     8101 aaaaaatcgt ttgttggatc agtagagaca agagttggag aaagtaattt ggttcattga
     8161 tttctatttc tttttttaga tcgttcagta aattctcttt ctgttgatac aagtgtgcat
     8221 attgctgatc atatgtttct aaaaaacgat ggtattgacg gtccaatgct tcaatcaata
     8281 tcggcgattc atgatatttt aatatgagga gagccaagtt atcagtcaga gatgtttttt
     8341 ttagttgatc aagagacagc tctgaagcta gttcctctac aaacgtttcg aggacctcta
     8401 aaaatggaga atgatgcagg cagcgagcgg tttcttgtcc atagttattg ttgtaattcc
     8461 aactttccag aaagcagaaa gaagcaaaag aaagctcagt gtctttaaga taaatacctt
     8521 ccgttaaata caattttttc aatgcagagt catacaggga ggagagtgtg ctgttggtat
     8581 ggatagaaga atctaatcgg caattcgctt ttatacggat cgtattgata aagaaccaac
     8641 atgttccgaa aatctcttct gtttcaaccg ctagttcagt ttgactgagg tttcttaaaa
     8701 attgaaaata atgggattca tggatcgaat aatcctcgaa aaaatagttg ttatgagtga
     8761 aaggaatcag caatcgatga taaaaagtac gaatcgcacg ctcttttcca agaatacaag
     8821 ctgtatattt cgtcaaatcg atctgcaagt cgtaacttgc taaacgctga ttgattttag
     8881 tcacatgtct tttcaatgtc ccataagaaa gcccatggtc ggctagaaaa tcagggacag
     8941 caactttttt tgtaaaaagc agatttttca gcagttcgac actgactgat tgtttgaaaa
     9001 aaatctccca aaggtcattt gttaataaat tttgctggtt ttgcaaactg attcctgctt
     9061 gatcgctgat gatgtcccag ccttcgggca gctgagagcg aatagattga aggtcagaaa
     9121 aaatcgttcg ttcggttgtc cctatgattt cagctaattc atttacggtt atctttgtat
     9181 gattcagcaa ttgttctact aaagaaacat gacgtcgaat atctttttcg gtaatgattt
     9241 ttttcattag tgaatacatc aatcagactc ccttctattc taagaatacg gagaaaataa
     9301 acggtggtca agtttcaact gatttttcaa tcattgttta tttttctgga gcatttactg
     9361 atgataagaa tttcatcatg aacttgaaaa atgataccac taaaatcaaa aaataggtgg
     9421 gcaaacaata ataaacattt taatagtaga tggtagcaag accaacacag gcggattgaa
     9481 catttaagag ttgataaaaa agaaaaatat cgaagtattt gattagcaac tacttgtatt
     9541 catttttcta ttcttacgtg gtatctttga ataaagcgaa tcaataagaa agagatggaa
     9601 gcagatgcta acgaaaaaag aactaatcga tcacatcaat gaaacataca atttgtctcc
     9661 agaatatatt tttaaaaaat ttcctgatta ttgcgtattc agacatcaaa aaagcaaaaa
     9721 atggtttggg gtcctgatgt ctgttccaga agaaaaaatc tatgatgatg caagtgacca
     9781 taggatcatc aatattcttg atttgaaggt cgatcctgaa cttggcggaa ttttgcgagg
     9841 aaaagagggg tattttccag cctatcacat gaataaagaa cattggatct ctattgatct
     9901 acgtaaaata gaaaagctag atgaactagc agatttgatt gatggaagtt ttaaatcaac
     9961 gcaatgaatg tctaggacaa tagagaagag gttgtgacag aaatgtttag cttcaagaaa
    10021 taagaaggaa tttacgaaaa ttgcttctca aatttttggg aaatttcggc ttattttcta
    10081 agaaattact tctggaa
//
LOCUS       DAZKNO010000098        10031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000098 DAZKNO010000000
VERSION     DAZKNO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.100"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            141..461
                     /locus_tag="ACG8MY_001826"
     CDS             141..461
                     /locus_tag="ACG8MY_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431010.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            913..1113
                     /locus_tag="ACG8MY_001827"
     CDS             913..1113
                     /locus_tag="ACG8MY_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0431011.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1260..4049
                     /locus_tag="ACG8MY_001828"
     CDS             1260..4049
                     /locus_tag="ACG8MY_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0431012.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4096..4623
                     /locus_tag="ACG8MY_001829"
     CDS             4096..4623
                     /locus_tag="ACG8MY_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0431013.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4644..5834
                     /locus_tag="ACG8MY_001830"
     CDS             4644..5834
                     /locus_tag="ACG8MY_001830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0431014.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGILELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8MY_001831"
     CDS             5874..7172
                     /gene="asnS"
                     /locus_tag="ACG8MY_001831"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0431015.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8225..8899
                     /locus_tag="ACG8MY_001832"
     CDS             8225..8899
                     /locus_tag="ACG8MY_001832"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0431016.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8896..9237
                     /locus_tag="ACG8MY_001833"
     CDS             8896..9237
                     /locus_tag="ACG8MY_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431017.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9267..9626
                     /locus_tag="ACG8MY_001834"
     CDS             9267..9626
                     /locus_tag="ACG8MY_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0431018.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
BASE COUNT         3346 a         1791 c         2148 g         2746 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      361 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      421 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      481 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      541 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
      601 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
      661 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
      721 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
      781 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
      841 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
      901 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      961 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1021 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1081 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1141 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagtatttt
     1201 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
     1261 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
     1321 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
     1381 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
     1441 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
     1501 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
     1561 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
     1621 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
     1681 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acgggtcaac
     1741 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
     1801 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
     1861 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
     1921 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
     1981 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
     2041 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
     2101 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
     2161 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     2221 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     2281 tcaaaagcta tcgccactat attgatttac agcgttttaa aggcacactt gtggaaccgc
     2341 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     2401 agaccggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     2461 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     2521 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     2581 taatacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     2641 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     2701 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     2761 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     2821 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     2881 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     2941 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     3001 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     3061 agatgcggat cgatttagca agtttatata gcttatttga tcaattagac aagcagtact
     3121 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     3181 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     3241 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     3301 gccctaatcg acactatctc tctaagcggc ttggcattga agcagcacct cttaaagtga
     3361 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     3421 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     3481 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     3541 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     3601 ggtttggcgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     3661 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggggatgct ttgcagatcg
     3721 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     3781 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     3841 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     3901 ttattttgga tcgaagacta gtcactgcaa aatatggtgc acggatcatc catgcattac
     3961 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggac atccatgaat
     4021 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     4081 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     4141 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     4201 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     4261 gtagaaatcg caaagaaata tgcaaatctt gaggaagccg atcattttta ttggtttact
     4321 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     4381 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     4441 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     4501 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     4561 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     4621 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     4681 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     4741 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     4801 tcgaagcaat ccgcaacggg aaagctagct attatacacc aacagcagga atcctagagc
     4861 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     4921 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     4981 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     5041 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     5101 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     5161 catcgaatcc gactggaatg atttatagca agaatgaact gcaggcaatc ggtgaatggg
     5221 cagtaaaaaa tgatattttg atagttgctg atgatattta tggtcgttta gtttataatg
     5281 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag actatcatca
     5341 tcaacggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggaa
     5401 atcctgtgat catcaatgga atgatcgctg ttgcttccca gtcaaccagt aatccgacag
     5461 cagtcagtca atatgcagct gttgaagcat tgaaaggcga gcaagataca gtggaagaaa
     5521 tgcggaacgc ctttgaagaa cgcctgaata cgttatattc gcttgtggca gaactgccgg
     5581 gtgtttctct tgaaaagcca caaggagctt tttatctttt tcctaatgta aaagaaacgc
     5641 tacgcatgtg taaatatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     5701 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     5761 ctactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     5821 aaagtcaaaa ttaatcctaa agaaattcaa ggaatataaa aggagagtca tacgtggaac
     5881 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     5941 tcgcaaataa gcgatcaagc ggaaaaatcg catttttaca attaagagac ggaacagcct
     6001 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     6061 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     6121 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     6181 cgatcacacc aaaagaacac ggaacagact tcttgatgga tcatcgtcat ttatggctgc
     6241 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     6301 aattctttaa tcaacatcat tttgtcaaaa ttgatccgcc gatcttgact ggttctgcac
     6361 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     6421 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     6481 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     6541 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     6601 tagcattctt agtacaaaat gttttagatc attgcgaata tgcattagat gtactaggac
     6661 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttatgatg
     6721 atgcgatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     6781 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     6841 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtga
     6901 tctgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     6961 caacagacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     7021 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     7081 gtcttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     7141 tcccacgttt gttgaaccga atttatccat aagaggtgat gaaaaagctg ttctgcatca
     7201 agtaattaga acagccaagc aaaaacagct gttcatgttt ttgaattggc gggacttatt
     7261 accgcagatg taagcttttg aataccgttt agtggagagg ttgtgaaaaa gctgtttagt
     7321 ttcgagtatt aagaaaaatt ttgtggaaat ggctttccac atttttgcat aaatttggct
     7381 taataccgag aaactggctt ttgaacaccg tttaaggaag aggttgtgcg cctgccgttc
     7441 agctccgagt cacaaggagt aatttcaaaa aacagttttt catgtttgat gactattgtg
     7501 acttgtgacc gaggagttgg ccggagagca ccgtttattc taaaaaagaa ctgcgaactc
     7561 tcgcagttct ttttttaaaa tacgggataa aggaagtttc cagcagacaa gagaagaaag
     7621 caaaagaaat tgctatttga ttcacaaggt tctataacga aatcgcaatc aatcaaacat
     7681 ttacaaagaa ggtaattcaa atggaaatct gatttgatga tgcttctgct gttgaaataa
     7741 agcagcatct agcacctgaa aacaaactaa tagtaacatt cgaagatgga gtaggtccgt
     7801 attttcagca tgcaataatc catatgtaag ttcaattcac ccttgaaatc atcggaccag
     7861 ataaagttat gaccaatcta tcccaagcaa tctagaagag atattagtaa agggacattt
     7921 actagaagtc ttagatgagc ctataaatat atgttctgat ggcgtcatca gtggtctgat
     7981 tgacggaagc ttaggtgtga tcgatctctc tggaccaaac aacgaaggaa tcaaggggaa
     8041 tccggcacgt taagaaaaaa atagcataaa aaactagaca cttgatgagc gttcattcaa
     8101 ttgtttgaag ggactaggct ggtgtcattt tttatacata tattttctct ttcgtcttga
     8161 tagggcttat gggataatat aaggaagaaa atcatcgttt tattcaaaaa ggagtttttg
     8221 acaaatgaaa aaacgtatat tatttatcgg tgatagcatt acagacgcag gaagagaccg
     8281 agcagaagca actgatctag gtaaaggtta tccacttttg attgccggaa aactaatctc
     8341 ccaatatcca acgatggaat ttgaatgcat gaaccgtggg ataggaggag acaaaattgc
     8401 agatctgcaa gtacgctggc aagaagattg tttagcttta caacctgact atgtatcgat
     8461 tttgatcgga atcaatgata cctggcataa tactagcgat caggctatat tcggaactga
     8521 gaaatctgct cagcaatttg aatccgttta tcgaaaatta ctaaatgaac ttcaaacagt
     8581 gggaatctgc aacatcatct tgatggagcc gtttgtactt cctataaccg acgaccgtca
     8641 tggctggcgt aaagacttag accctaaaat ccaaattgtt cgtaaacttg ctaaggaata
     8701 tggcgcattg ctggttcctt tagatggtat attcaatgct ttagggattg tttatggttt
     8761 ttcgaaatat acaagagaag atggggtcca cccaacgatt ggcggacatg agatcattgc
     8821 acaaagctgg atagatgcaa ttgccgcagt cgattttttt agtggagata aaaatgagcg
     8881 aaagaaaagt gagaaatgat ggaaatacgg tgggcagtaa aagaagatct acctcaaatc
     8941 aatcagctga ttactgatca tccagaatca ttcgtaaaag caaaaacggc agctgaccta
     9001 aatgataaga ataaactatt gctcgttgct gtaacaaaag aaaaacggat tatcggattt
     9061 gcgcaagcgg acgtgcaaca gtcagaagaa cgattggatc agcgaccatc aggaaaacta
     9121 atccaacaca cattagtaac aaaagaaaat acggaagaaa tccaaaaaaa atttgcagaa
     9181 tatttgatac tggaagctaa aaaaagaaga ataaaatcat tttttgccca gttttaatca
     9241 aaaacagtaa tagaaggaga aatacaatga gttgtttagg aaatgtcatc tggtttatat
     9301 tcggcggact aatcggtggt cttagctggt tagcagcggg gatactttgg tgtatcacga
     9361 tcataggtat cccaatcggt atgcaatgtt ttaagatcgc ctccctttct tttgcgcctt
     9421 ttggcaaaga agtaatttat caccagtctg gcatgtcatt gattgttaat atcatctggc
     9481 tgatcgtttc aggcctacca attgctctcg cacatgtcgt cagcggcatc cttctatgta
     9541 tcacgatcat agggatacct tttgccaagc aatctttcaa acttgctcga ttggcgttga
     9601 tgccattcgg tgcagaaatt gtataaaaga gaataataaa ggttagataa agcatagagt
     9661 caggtgggat acatgtattt cacctgactc tatgcgttct cctgtataag ttcttccatg
     9721 tgacttttac gtcttttatt tatatggggt tactgtttat ggaaagttga aataattttc
     9781 attaaagaac tctttctata tttttgaaag cgtagtggag cttcgaatat ctgggaagga
     9841 cattttgcag ggattgaaaa aagatattgt cctatcgaaa aattaatgaa tttagacaaa
     9901 ttgccagcaa caggcttcaa acttagttgc tatcctgtca agatcaagaa ggcaagtgcg
     9961 ggttggataa gagcagtggc aatgatcgaa aagaaaaaga aagaataagc caaaacatct
    10021 gtatggaaga a
//
LOCUS       DAZKNO010000099         9984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000099 DAZKNO010000000
VERSION     DAZKNO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.101"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..139)
                     /locus_tag="ACG8MY_001835"
     CDS             complement(<1..139)
                     /locus_tag="ACG8MY_001835"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0431019.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CM"
     gene            complement(285..1163)
                     /locus_tag="ACG8MY_001836"
     CDS             complement(285..1163)
                     /locus_tag="ACG8MY_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0431020.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1202..2320)
                     /locus_tag="ACG8MY_001837"
     CDS             complement(1202..2320)
                     /locus_tag="ACG8MY_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0431021.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2317..4416)
                     /locus_tag="ACG8MY_001838"
     CDS             complement(2317..4416)
                     /locus_tag="ACG8MY_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0431022.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4467..5288)
                     /locus_tag="ACG8MY_001839"
     CDS             complement(4467..5288)
                     /locus_tag="ACG8MY_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0431023.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5285..6094)
                     /locus_tag="ACG8MY_001840"
     CDS             complement(5285..6094)
                     /locus_tag="ACG8MY_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0431024.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6115..6609)
                     /locus_tag="ACG8MY_001841"
     CDS             complement(6115..6609)
                     /locus_tag="ACG8MY_001841"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0431025.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6621..7121)
                     /locus_tag="ACG8MY_001842"
     CDS             complement(6621..7121)
                     /locus_tag="ACG8MY_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0431026.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7186..9882)
                     /locus_tag="ACG8MY_001843"
     CDS             complement(7186..9882)
                     /locus_tag="ACG8MY_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0431027.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3019 a         2252 c         1647 g         3066 t
ORIGIN      
        1 acatacattg gtaagcaact ataactatta ccacagacat aaagtaacac aatatattac
       61 tatttaagtg tacgccacca gtgaatatcc tcaaggtata cagcgcacaa caaaaagaaa
      121 aaaggaaacg cagaagcatt tcctttcagt gatttaatga aaacaacacg atttgactta
      181 gagccttttt atttgaattc attttcgctt tatccaactt catttgatca tttgattact
      241 cggttaaata cttttgacat gagtatcaat gaaacattca ttaattatgg ttgcttattt
      301 cttaccgctt ttctggccaa tgctaatccg ccgattattc cggcattatc acctaattcg
      361 ggcgtaataa tgtagctatc aattggtggt atttcaacat atccatttat aagctcaaca
      421 aatttatcct gaactttttt cttcatgtgt tcttgtttca ttacaccacc gccaaaaata
      481 attctatctg gcgaaaacat cagtgttgta ttgtatgcac attgggcaag gtaaaaggct
      541 tcaatctgcc aaaacgaatc gtcagccaat aaattctggc cttttacacc caaccgtttt
      601 tcaatcgctg gtcctgctgc catcccctct aaacaatctt ggtggaaggg acagtttcct
      661 gaaaaattgt cctttggatg tcgcttgatc aacatatgcc ccatttccgg atgactgaac
      721 ccttccacga aagtcccatt gaacaaggca ccacctccga tacctgttcc cacagtgaag
      781 tatgcaacac ctgctaaccc tttgccagaa ccagcaacat attcaccata gcaagccgca
      841 ttgacatctg ttgtccattc aatcggaata ttgaaatatt gctttagcgt tcctacaaaa
      901 ttgaaatttt gccaagcaag ttttggtgtt gatgtgatat gaccgaaact cgttgatttg
      961 cattgaatat caattggacc aaagcagccg accccgatgc ctgccaattg ttccttaaaa
     1021 ggtttaaaaa aatcaatgac ctgcttcatt gtttcaactg gagttgttgt gggaaaactg
     1081 actctttcca aaatcgttaa ttcctcagta ccgactgcac agacaaattt agtccctcca
     1141 gcttcaattg ccccatatat catcataggt tcctcccttc gaatatgtga aaaacacttc
     1201 attaacgcgg caagcgttct cgatcagtga aatgacgttt agtatttcct gaagcttctg
     1261 ttgaatacca gtaagcgaca ctagcaacat cgtcttgccg ctcaaataag cgaacatcat
     1321 cgttgccgat ttgttgtaaa gtgaccgata aatcttcggt aaaacagatg gggtcttgaa
     1381 gatgccaacg ataaagacca tgtcgtggca ttgcgtcgtc accaaaggca tgaacggcat
     1441 tcaattttcc cgtttcaaaa cgatctcttg tggcatctct atttgattgg taaggatacc
     1501 ctaaaaaggc tgttgaatag tttttcgcat gtggacgtcc gaactcatcg tactcatgaa
     1561 aggcccacgc cccgccaaaa taatcttctg aacctgttga ggttacggtc ggatactcac
     1621 tatccccatc aatgtagaat ttaaactcgc cttcgcccca ccaataacgt tctaggcagg
     1681 taagcgcaag gtaagtaccc acgtaatagc cttgaccctt taatcgatca attaacttat
     1741 aatcctcctg taatttggta atgcgttccc gatgccaatt tgcgtgaaaa tataggctat
     1801 tttcttccaa ttcgtccacc aaagcgtaat ttatcgtata aaaaaatgag gacatatcct
     1861 tcggatgtaa attctcgatc gtaattttag cggatgtttg gaaaggcatt tcgaaatagg
     1921 aattaaagcc ccctgtagga ttaaccacta tcggcaacga gttcacctcg caacgctctc
     1981 caaacccatt acaaaagaaa tcccctaaag gactttcaac agcagggcta tccgacccat
     2041 cccaataaat tcttaagatt aaatcacgta aaacaaaact gccaaattct gttcgatcac
     2101 gaatcgtcat ccacatatga cgaattattc ccgttccagt aatatccgca atcacagttt
     2161 cttcaccaga agtcagcgga attgcgccac gacctttgcg gcttggtcct agctcgcttg
     2221 gcaacatcgc agctttacct ttctcgcctg ttggattttc gggagtgatt gctcgactct
     2281 ggattttttt aaattgggta atatttttta gtatactcat ctttttctcc tttgaatgtg
     2341 aatcgttttt cccttttcta cgtgaagaat ttttttcgta tcacttattt tcaatggctt
     2401 ttctgacaaa tttgaaatac tcaactcgga ttgcgtacat tcaatcgaaa tgaaggcttg
     2461 accgaacttc aaattcttcc aagaaaacgt taaattatca acaggatcaa tttgcaatac
     2521 tccttttttg aatttcaatc cgaaatattg actgatcatt aggctgacaa aaagacccga
     2581 tgcccagccg catttgccgc agccaaagtc tcgcacgact tctccttgtt gagcctctaa
     2641 gcgatacggc caccaccacc acgaaccgtc taaatctgcc aaacaaatta gttcttctaa
     2701 tcgggttacg aaagcttgag ggttgtgcag ctcgctcatt aaaacagtga taaagccagg
     2761 aaaagtggcg ccggattcta atccccaagt gatgcctttg atttcctcgc tataggttgg
     2821 attgaaagaa ctagcagaaa aggccatggt gttctgatat aaatgatctt tattatctaa
     2881 aaattgataa aaaggcatca acgtagtgtc tgattcttct ccatcatgca tcaataaatt
     2941 aatttttcca tccacaatca catcacttaa aaagattaac ccttgctcaa gaatcggttt
     3001 ttgatagtgc ttcactgaat aggttgtttt ctcctcatca ccgattccct ccaaaaattg
     3061 ctcaccaaaa ggaccatcga tagtcatttg tttacaaatt gattccttta gtttacaggc
     3121 aatgtttttg tatcgcttgc taagactcag ttgttctagg gcttcaaaaa tttctgcaag
     3181 tccaatacag gccttccaaa gacaaagatt gctgccggta tgatactttc caagtgcaaa
     3241 ggcgtctgat aaccaaacag atggaaaaag cattggttcc cctgaccgac gttgagctaa
     3301 tatgtcctca ataattcttg aagcattcag tagaacctgc ggacaccgct caaagaattt
     3361 tgtatcccca gtatgttcaa aataaagact agccagtaac gcactggata acgaattact
     3421 cagagaatga ttgatacctc ctgtaaattt tgaaccagga aacttaacgc catattgatc
     3481 aaaccataga accgtttttt gacataactc cggttcaaac atggtgaatg gcaatgatgc
     3541 gtaatacata tccttattcc aaattcgatt agtagcagga tagctgcccc aattgctgcc
     3601 gacgacctgt ccctgtcgat tcataccgaa actagaaaag ctttgataaa gtgcacgacg
     3661 atagagatgg acaactctat cgtttggcat tgacagctgc ccaaatagct gagcaaaata
     3721 gttcaatgtt tccttcagcc atttttcgat atcagattcc tgaaaagtca ttacttcttc
     3781 aaaagcattt gggtccacaa aagcaatggc gaagcttgcc acttcctgtt gtgcgatcgt
     3841 gtccttattt cctttttgag aaatcaacac attttgctga tccgaatact tctcttgata
     3901 cgaaggcaca tctttcactg aaacttgaag gctttcgtgt gtttcattca caatgtaaac
     3961 ctgttgaaca agcatactta aacgcttatt agccagtatg ggagcaaagg atactacaat
     4021 gaccttaaac gtcgaataat gatgaatata tctaggcaaa cagtcttgaa tcacatcgac
     4081 ttcaatacga tgatccgatt ctgacaaatg gtagacaatc ccgtttactt caacctttaa
     4141 ggataacgaa ttggtttgag agagctgttt attgacccaa acacctggtt tttcctcagt
     4201 tagtaaattt tctcgataaa aggtcaacgt ttgaatcgtc ccgcaaacat caaggtttgc
     4261 taatgtcttt tgattactta catcgaaggt caatcccttt acatcaggaa tattccgata
     4321 gtagctgtat tcgtttgaag tagtcacatt tccgtcgttt aactcagcat taaaaaatgg
     4381 gtgtttgggt gaataaatta cttcattaat agtcatcttt tccctccttg ctaaaaaaac
     4441 tgcagcctag tagctgcagc ttcctactat gcgattccaa gtaatgacaa taaaattccc
     4501 aagacgacca ttccaaaaat aattttgatg acgccgactt ttttatctag taatttgaaa
     4561 cagctcaatg ttaccaataa cggtacaatt cctttaaaaa tactatcgag gatttcttgg
     4621 acattcatta gttcttcgcc cttcattgaa aagctcaaaa cggtattgaa tttgaccata
     4681 ctgatcgtca ttgcacctac catgatcaga cccaagatac tagcagcttt tgtcagaatt
     4741 tcaattaacc cgttcgcata cagcttttca atatatttag agcctaatga atagcctaaa
     4801 acgccgccat agtatcgcat caagtaatta ggtatattga acataactaa aaagataatt
     4861 ggtgccaaaa tatttccggc attggctaag ttgatcgcta gtcccgcggt aacgacacgc
     4921 caaactcccc agaacaatga atcgccaatt ccagccaatg gtcccatcaa actcgttttg
     4981 atcgcgttga tactttccac gtcaaaacca gctttttcag aattttcctt ctccatcgat
     5041 gccgcaatcc ccatgacaaa ggacgcgcaa ggaatggtcg tcgaaaaata ctgtgtatga
     5101 cgttccagcg ccgcagctcg atcttcttca cttttgtaga aatgattaat gaagggcatc
     5161 atagaatagg cgaaaccacc gccgcccatc gtcaccggat tcaccgaagc atacatagtc
     5221 atcgagcgcc aaaacatctt ccgaaccaat tttttctcat ctttagaaat tccatcagct
     5281 aatttcattg aaagaaatcc tcctcttctt ggtcaagcgt tgcatttaca gcagcatcgc
     5341 tttgaactgc atgaacagat atatttttta gttgtagatc tctttgggct ataacaattg
     5401 caataatcgt tccaactgcg gcaattgcaa tcaatggtaa ctcgagatac gccataacaa
     5461 cggccccaaa gaaaaaccac acagcaatat ttttactcca aagcatccgt aacagcatcg
     5521 ccatcccaac tgccggcagc agatttccag cgactgttaa tccgttcatg atgacctctg
     5581 gaatattatc caagacgctt ttgacaacat ctgctccaaa taaaacggca aagaatggaa
     5641 taagagcata aagcgcatag tggacaaacc acataaagaa gtgcagattg atgattcctt
     5701 tttgatcacc agattttgca aatttgaaaa atttagcagc catcggtcca catacaaaag
     5761 aaaaaatcat attcattaac tgtaatccta atactgctag tggaaagaca atgggaataa
     5821 tcgcatccac gtttcctccc attttgattg cgaacgctgt agccagccca gcagccaaag
     5881 aaggctccgc cgtactagag attccgatat tgacgacacc cataaagatg gcttctagtg
     5941 acgccccgac aatcaacccc gtctgcaaat cccccatcaa cgctcctgct aacggagcaa
     6001 caacaatcgg acgactcagc atggacatcc cgaacatttc atgtccagca actccaataa
     6061 aaacggccaa agcgactaca aatgcatcat acataatttt tctccttcct ttttctaaaa
     6121 atcactcaaa atagagatag ctgaacgttt ttccatcgcc ggtgtaggct gaaaaacggt
     6181 atctggataa agttcaacta attcttttaa gtcttgaatt tctgacgtcg ttaacatcac
     6241 ttcctttgat aaggttttct tatcttccga tggcggtgtc attttcccag cattccccac
     6301 gttcattaat tcgatttccg ttgggaaagc tttcgcaact tttaccgcat ctttgacatt
     6361 agggactaaa accatcaagc gcatgtcctt cgcccgcgga tcttttaaca aatgaatcgc
     6421 gtcatcaact gttttaataa tgactttgac attcgatggt gcagccatct tcaatgtcat
     6481 cttttgaata tcgttggtag ccgcctcatc gcttgctact actaacccgg ttaaatttag
     6541 actctttgtc caagtcatcg ctacttgtcc atgaattaat cgttcatcaa ttcgcactgc
     6601 tttaatcatt tttcttcctc ctaaaaaaaa tcttcctctt catttactac atgtacctca
     6661 tcaagaatgg tcatctttaa ttcctgttgg catttattta ccagttcctg aatatcctcc
     6721 tttgttaatt ccttggttgc caatactatt tcgagcagaa ttggcagatt aaagccagac
     6781 aaaatagtta cagcaatctc tttctccaaa aaggttttgg taatcttctg attgacactt
     6841 ccgccaaata aatcggtaaa aacaaaaatc tgatcctgct cgttcacctg gtttacaaat
     6901 acattcaact cctgttcaaa atcacactca tccacataag cattaatcac cgtaatctct
     6961 tgaaccttcc ccgtcagtaa ttgaatactg ctaagaaagc ctgaagctaa gtgaccgtga
     7021 gtcgctatta gaattttctt catatggtta attcctttct ttctgttcac ttttatataa
     7081 gcaattcctg tgccaacttt taaaaacatt ggtttaatca cgttaattca cattaaaaaa
     7141 agtgtctaac ctaaaaaaaa cagattagac acttttttag acactttatt tttgaatata
     7201 cggtcctaat aattcataga gcagcgaaag ctcataagca ttgactttaa aattatattt
     7261 catctccatt gattgaaaaa tacttctaga aacataataa aattcatctt catctggttt
     7321 caaatcataa ttcggttctt cagatttttc tgtccgagcc aagaataaac gttccatcat
     7381 caatgccgta tgcatataga gatttagttt tacacggcca tttaaagtga aatgatagta
     7441 attttcatat ttagaaatga ctgtttccac ttcactcatc acgatcgttg ggttcagaaa
     7501 gttcaaacgg tccgcaattc cttccaacga aaagaatttt actagctcct gaatcaagcg
     7561 gttaaattct gccggcttca tttcaggact caaaatatgc tccaaattgg ctgctccatc
     7621 accgtctaag atgtcataaa cattaatatt aggaattgaa aaggaacgtg gaagatccga
     7681 agtcgtgatg accaattttg tcttgtcaaa ataagtttca tcattttggt cgattaattc
     7741 acgtagttct ctgtagtcct tcgtgaaaac ttcgatggat tcatccgata aatgatgacg
     7801 aaaaacatct ttcaatttgt ccgaaatccc caaacccgac atacaggaaa tgataatgtt
     7861 gctactttga gaaaagcctt caaagtattg aacattgatt gaatacttgt ctgctgcatc
     7921 ctccgcaatc tgtttaaatg gcgcattcat cgtcatcttc aagccaatat caagtgcaat
     7981 ggaagtcgtc aaattattca gaagaagtaa ctcccctttt aaatcgttct tgatctgtgt
     8041 gtaaatttga tttaaagagc ccatatctac gatcagcact agactatctg tattcacttg
     8101 ccgtttcacg aaattccttg tcgattcgac aatctctgac acattactct ctactggcat
     8161 atccaacgct tcaaaaacat aattgccgca taattgattc actactgctt gaatgctgct
     8221 agccgtacta tctccgtgag caagcaacag gcccttcagc tcgatggatt catctacata
     8281 atcagataaa gtcacgacaa taagcagtct tagaaagttc tcgtcctttt ctgagagcgg
     8341 tggtaaatgc tccaagaact gattacataa gtaagtcgtc cgaggtagat ttgcagctaa
     8401 tgtacgattt atcccggtta agtctgtctt atagcttggg gaaatgacat aggatctgct
     8461 aataatttct gcaacaggtc cactcgtcgt caatccgtac gtttttccta aaacggatac
     8521 ccagaccttt ctaaacatct gaatcatcaa ttttctactc attgataaaa aagaatcctt
     8581 atcaatgtga tgcaccaaat gctgtacttc attctttatc gaagaaaaat tttcattgaa
     8641 attaccttca tttatcagct gagtaatttt ttccaaaatc gaatcataat caaacggttt
     8701 gattcgatcc gtttttatct tttgattttt ctttatcaat aaactagcaa tcggcaagct
     8761 ttctacttcg gattccagca tatttaactg aatcaagatc tcatttttcc caatcgttaa
     8821 tggttcttct tgtgttcgac tatacacatc cgcacaactg attttaatga tattcttcag
     8881 cttaccaata ttcccactaa agggatgaga taacattaaa gacaaggctt ctcttgaaac
     8941 aataatatct ttttgtaaaa catccgcttc attttgaaaa aataattgaa tcagctctaa
     9001 tcgttctcga atggggcgat cttcaaaagt tggcaacata acactaattt gaatacgccg
     9061 atcaaaggtg tctaataaat aattttcacc tttttccgtc gtggcgaaaa taaaacgaac
     9121 cttagcagaa tgccaattta cattttcacc gactggacga tagtttccag tatcaataaa
     9181 tagaaagagc ttttcttgat tttctttcga taggcgatgg acctcatcga gaaaaagata
     9241 gccgttgttg gccgttgcca ataaaccatc ggtatccttt tcagcccccg taaaggcccc
     9301 cttcttgtat ccaaacagtg ttgccgaaag cagttcggga ttgtccgcat aatcggcaca
     9361 gtttaagata gcaaatggtg catctttttc aataatttgc tcatggatag cgtattcaaa
     9421 aatttttgtg gctaaaaaac tttttccaac acccgtggct ccatttatta atacactgag
     9481 tccgttaggc ggatatttga cagcggctat acatttttga atgacctctc tcagacttcc
     9541 gtcatgacca actacagaat gaaaggcttc gtttatttgc tccgtattat ctacaaccgc
     9601 atattcatct aaaggcttcc acaaaacagg ctttcccggt aatttctcga cctttttatc
     9661 ttccactaat ctcgtcaaat aatggctggc attctgacga gagataccta gattcttcga
     9721 aattttctcg gcagtgacag aagacatatt ccctgttagt cgtatcagct catcaaaaac
     9781 tcgatcttta gcgctctttt tcattttttc ctccagttta atgtgtctaa aagcataata
     9841 aactatcggc tgcaatatgt ctattctata tttgtattcc aatttttact ttgaaacaca
     9901 aatagtcgaa tatttgcctc agatatctga caaaataatt tatctaccaa tttagttttt
     9961 atctaatatg atttttatgt tttt
//
LOCUS       DAZKNO010000100         9949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000100 DAZKNO010000000
VERSION     DAZKNO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.102"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..1173
                     /locus_tag="ACG8MY_001844"
     CDS             175..1173
                     /locus_tag="ACG8MY_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0431028.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1285..1794
                     /locus_tag="ACG8MY_001845"
     CDS             1285..1794
                     /locus_tag="ACG8MY_001845"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0431029.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1824..2483)
                     /locus_tag="ACG8MY_001846"
     CDS             complement(1824..2483)
                     /locus_tag="ACG8MY_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0431030.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2485..3249)
                     /locus_tag="ACG8MY_001847"
     CDS             complement(2485..3249)
                     /locus_tag="ACG8MY_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0431031.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3257..3934)
                     /locus_tag="ACG8MY_001848"
     CDS             complement(3257..3934)
                     /locus_tag="ACG8MY_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0431032.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3971..5356)
                     /locus_tag="ACG8MY_001849"
     CDS             complement(3971..5356)
                     /locus_tag="ACG8MY_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0431033.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5566..5650)
                     /gene="ffs"
                     /locus_tag="ACG8MY_001850"
     ncRNA           complement(5566..5650)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG8MY_001850"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5673..6188)
                     /gene="tadA"
                     /locus_tag="ACG8MY_001851"
     CDS             complement(5673..6188)
                     /gene="tadA"
                     /locus_tag="ACG8MY_001851"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0431034.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6295..7152
                     /locus_tag="ACG8MY_001852"
     CDS             6295..7152
                     /locus_tag="ACG8MY_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431035.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7233..7568)
                     /locus_tag="ACG8MY_001853"
     CDS             complement(7233..7568)
                     /locus_tag="ACG8MY_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431036.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7635..9005)
                     /locus_tag="ACG8MY_001854"
     CDS             complement(7635..9005)
                     /locus_tag="ACG8MY_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0431037.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9083..9619)
                     /locus_tag="ACG8MY_001855"
     CDS             complement(9083..9619)
                     /locus_tag="ACG8MY_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0431038.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2971 a         2035 c         1650 g         3293 t
ORIGIN      
        1 ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt tgttttcttt
       61 tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat tcaaacattt
      121 ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat ttttatggaa
      181 atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc tttttacgca
      241 ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg tggtcaatta
      301 gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc aataaaagca
      361 agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac attccattta
      421 aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac tacaaataaa
      481 ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt ccagccaaga
      541 aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta tttcgtgaat
      601 gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga ttctgctatc
      661 gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca tcgtcgcccg
      721 gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt caacttgctt
      781 actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga tatccggtta
      841 aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat cgtcaattat
      901 ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac cagaaatgcc
      961 attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc tggtgacatc
     1021 tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc acctactgca
     1081 gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg tcacagcaca
     1141 agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg catttcaagt
     1201 taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa atagagaaag
     1261 aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga agaaaaggca
     1321 agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact tgtcatgttt
     1381 ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag tgtcaacagc
     1441 gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt agatatatta
     1501 gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga cgaaggcctt
     1561 tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac gatcggcttt
     1621 acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact aaacagccac
     1681 gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc cgctgctgct
     1741 gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca ataattcaac
     1801 aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg tttcaagcta
     1861 ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa aaataagata
     1921 aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt aacgggatcg
     1981 aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa gcgatcaaat
     2041 cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg aaacggacga
     2101 atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat actcgggatc
     2161 aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc ataaaagtga
     2221 tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt gaagggatta
     2281 ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc taaagaagta
     2341 taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa gttgatcgtc
     2401 acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc atattccatc
     2461 cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt gtttcacata
     2521 ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg ataaaacgag
     2581 caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt tatctccaac
     2641 catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca taatgatggc
     2701 ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa aagagatgac
     2761 tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg ggttcgttcc
     2821 aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt cataggtcag
     2881 ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg taaatccggc
     2941 agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt tcccttttcc
     3001 atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct ctggagctac
     3061 atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact tagtcgtgtt
     3121 attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa atcgtttacc
     3181 ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta aatatccttt
     3241 atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt tttgtttttt
     3301 ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct gactctgttt
     3361 ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt ttctcatcga
     3421 tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt tcttttttac
     3481 cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg taaagatagt
     3541 cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct tggaaaaatc
     3601 cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat cgggctagta
     3661 catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa tcggacacaa
     3721 gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc actagctcgc
     3781 ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct gtcagttctt
     3841 cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct gttttatgct
     3901 caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc ttttttatta
     3961 taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc acacttcgga
     4021 taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga aaaaatggaa
     4081 caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg attggcttgt
     4141 tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag accatctgcc
     4201 caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa aagttacgat
     4261 ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg acatttaaca
     4321 agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc ccttttggaa
     4381 tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct cgttctttca
     4441 ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga ctcagagaaa
     4501 atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct agataactat
     4561 ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg gctttggctg
     4621 aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg acatactgcc
     4681 cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct ctgaataaat
     4741 gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca atttggaaat
     4801 catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa cgtaaatctt
     4861 cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc ggattataag
     4921 taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt ttcgttgtaa
     4981 tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg aataagttat
     5041 ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct ttggaatttc
     5101 ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc atcagcaaga
     5161 tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca aaatcaccta
     5221 gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta cgttgatcca
     5281 aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc gtatgtaaaa
     5341 tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc ataggcagct
     5401 ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag caaattgtat
     5461 aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat ttttacagta
     5521 ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata atcagacaca
     5581 tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca agcccaccat
     5641 tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga ccagtctttt
     5701 tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat catcccacat
     5761 tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc gtcctccaaa
     5821 agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa ataaacttct
     5881 gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa ggtaacaaaa
     5941 agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt gattgcaagc
     6001 atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc tcttccaatc
     6061 acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat ttctgctttc
     6121 tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc aagatttgta
     6181 tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca tatgataaaa
     6241 aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa caagatgaaa
     6301 atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc agcatcagaa
     6361 gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca agattcatta
     6421 tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga tcaaaaaaaa
     6481 cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa aagctttcgt
     6541 ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg gcaagatact
     6601 attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac agatgcgctc
     6661 ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg tatttttcta
     6721 tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt ccattcacct
     6781 ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac agagcaaaac
     6841 aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca ttactataca
     6901 aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat caaacaaaat
     6961 gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag ctctgctgca
     7021 aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt tcaagaacag
     7081 tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt attgcttcat
     7141 gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca accccttatt
     7201 tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac aaaatgcatt
     7261 agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac agagattttc
     7321 ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc actatctgaa
     7381 agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata gacgaatttt
     7441 tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc cgatacaacg
     7501 actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac ttttgttgat
     7561 gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac aaaaaaataa
     7621 acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt tactgttcct
     7681 tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac ccatccttga
     7741 ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt ttgagtgatc
     7801 ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata gctaggttca
     7861 aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt taaaccatcg
     7921 agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa aagtcttcca
     7981 gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg ctcttttgtc
     8041 atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt ttgtccatga
     8101 aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca tatacgtatt
     8161 gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac ggattgagtc
     8221 aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat gatctcttca
     8281 ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt ttttagcatt
     8341 tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg agattcttct
     8401 gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga acaaggagca
     8461 tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc gggtgcatga
     8521 tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa aatcttcgca
     8581 cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg agcagctaat
     8641 tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc gggttttgct
     8701 gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata cccagcttga
     8761 tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta gggagcgggt
     8821 tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt tagtggattc
     8881 caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc ggatgcttct
     8941 tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt cgcttcctcc
     9001 ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata aaggtggctg
     9061 atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt tccaagtatt
     9121 catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa tcttgagaag
     9181 aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc gacttttctt
     9241 ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat agcgaatcca
     9301 tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa aaaaaatctt
     9361 tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag tagttttcaa
     9421 aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat ttgaaatgtg
     9481 tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca gtaattgact
     9541 tcattccata ttcttccacg tataccatac ttttgaacag caaatattct ttactataat
     9601 ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata aataaataac
     9661 ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta tatccattaa
     9721 aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta ggaaataaac
     9781 aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag aaggcgacac
     9841 tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc taactatata
     9901 caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaa
//
LOCUS       DAZKNO010000101         9877 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000101 DAZKNO010000000
VERSION     DAZKNO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.103"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1482
                     /locus_tag="ACG8MY_001856"
     CDS             <1..1482
                     /locus_tag="ACG8MY_001856"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0431039.1"
                     /translation="RHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDSRKADASTL
                     FFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLAMAFYDYPQ
                     NKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFKSHLTTPES
                     LDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHISPIEHPTF
                     DDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESNYQVIRSDK
                     GTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQDAQDGLKEA
                     RVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGSPGNKAISR
                     RHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMDRPEAIRRA
                     LAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1566..2918)
                     /gene="celB"
                     /locus_tag="ACG8MY_001857"
     CDS             complement(1566..2918)
                     /gene="celB"
                     /locus_tag="ACG8MY_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0431040.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(2918..3436)
                     /locus_tag="ACG8MY_001858"
     CDS             complement(2918..3436)
                     /locus_tag="ACG8MY_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431041.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(3452..3778)
                     /locus_tag="ACG8MY_001859"
     CDS             complement(3452..3778)
                     /locus_tag="ACG8MY_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0431042.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(3791..5770)
                     /locus_tag="ACG8MY_001860"
     CDS             complement(3791..5770)
                     /locus_tag="ACG8MY_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0431043.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(5791..6105)
                     /locus_tag="ACG8MY_001861"
     CDS             complement(5791..6105)
                     /locus_tag="ACG8MY_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0431044.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            6273..6566
                     /locus_tag="ACG8MY_001862"
     CDS             6273..6566
                     /locus_tag="ACG8MY_001862"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0431045.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(6644..7756)
                     /locus_tag="ACG8MY_001863"
     CDS             complement(6644..7756)
                     /locus_tag="ACG8MY_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0431046.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            7909..8757
                     /locus_tag="ACG8MY_001864"
                     /pseudo
     CDS             7909..8757
                     /locus_tag="ACG8MY_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9110..>9877)
                     /locus_tag="ACG8MY_001865"
     CDS             complement(9110..>9877)
                     /locus_tag="ACG8MY_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFQ0431047.1"
                     /translation="ERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVE
                     LTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLA
                     PGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSG
                     NKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         3110 a         1995 c         1673 g         3099 t
ORIGIN      
        1 cgtcatttac tattaaaaga aaatctatta aaagaatttg tttcaacaag aggctggcat
       61 cttgatgtac cagaaaaagc tgaatttctt cagttaagtt atgattcaag aaaagctgat
      121 gcttctactt tgtttttctg taaaggaatg aatttcaaag aagaatattt ggattcagca
      181 atcgaacagg gtattcaata ttatgtatca gaacaaccat acgaaaattc agcagtagga
      241 atcatcgtaa cggatatccg taaagcaatg gcgcttcttg ctatggcttt ttacgactat
      301 ccgcaaaata aattgaaagt gatcggattc actggaacaa aaggtaaaac aactgctgcg
      361 tactttacaa aagcaatctt ggatcacaca acaaataaaa aaacagcatt gctttctact
      421 atgaatacaa cgttagatgg caaaacatat ttcaaatccc atttgacaac gcctgagtca
      481 ttagatttat atcgtatgat gtcagaagca gttgaaaatg gtatgactca tctagtgatg
      541 gaagtttctt cacaagctta caaaacacaa cgcgtatacg gcttgacttt agatgtcgga
      601 atcttcttga atatctcgcc tgaccatatc agtccgatcg aacatccgac atttgatgac
      661 tatttctatt gcaagcgaca attgatcttg aattcaaaaa cagttgtttt aaatcatgaa
      721 agtgactatt ttgatttact tagggaaaca gccgatctat tccagattcc aacgatcact
      781 tatggtcgtt cggaagaaag caattatcaa gtgatccgtt cagataaagg tactcacggt
      841 tttactttat cttctcatga agatcgctta gctatctgcg atactgctta tgatattttg
      901 ctggctggag gattcaacca agaaaatgca gccagtgcga tcattgccgc agcactaaca
      961 ggtgcctcca gtcaagatgc acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg
     1021 gatcaattga tccaatccaa tggagcgcat gtctatgtgg actatgcaca taattatcta
     1081 agcttgaaaa cattgctgaa atttgccaaa aatgaacatc cagatggccg tgtaattgtt
     1141 gtattaggca gcccaggaaa caaagcaatc tcccgtcgac atgattttgg aaaagtgcta
     1201 tcagaaacag ctgacatcgc ctttttgacg gcagatgacc cagcatttga agatccgaaa
     1261 aaaatcgcgg aagaaatcaa tgaagcaatt actaatccag atttgatcgt tcattatgaa
     1321 atggatcgtc ctgaagcgat tcgacgcgct ttagcagaaa gcgctccgca agattcagtc
     1381 gttattgcgg gaaaaggcgt ggatccctat caaaaaatca atggtgtaga tgaaccttat
     1441 gaaggggatt atgcaatcgt gaagcggttg attgaagaat aatggtattt atatactagt
     1501 ggtttcccat ttctaagaaa aaaagggact gtgatttttt gtcacagttc cttttttctt
     1561 agaaatcatt ttaacttttc actagctaat tctgctgcct tttctctttc ttcatttaat
     1621 aatttattat catagaattt gataaatggg aaccagataa tgaatgcgat gattgcacaa
     1681 atgactgcta acacagcagc tttccaatca ccaccactac caacaaatgc tccgatcccg
     1741 actggtgaag gccacggcat ctgtgcaagc atcggtttga cgatttccag ccgaatggca
     1801 aaataagcga gtgtagcaga agccatcgga gctaagaaaa acggaaccgc taaataggga
     1861 ttgtagacga ttggcatccc gaaaatgatt ggttcattga tattgaataa tcctggtaca
     1921 agtgaggctt ttcctaacac tttcagctga tcagatttcg cgaggaatgc aataaagatg
     1981 atcaatccta aagtcgcacc tgatccacca acggttacat aagaattgtt gaattctcct
     2041 gccaacggat aatttgcgcc ttgtgcgtta gctgccatat tagctaatac gatcggggtc
     2101 aagaaagaag tgatgatatt cgctccatgg atccctacga tccacaatgc atggatcaag
     2161 aaataaatca ccatgacacc aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg
     2221 atagcaatca ttttgaagat atccgttcct agtgcaacta ataatccatt gatgatcatc
     2281 accacaaacg caatgacaaa tgttgggatc agagcagtga aagaacgaga aactcctggc
     2341 ggaacaacat ctggcatttt tatgacccaa tttctcttga cacacataca atacaattcg
     2401 accgcaataa tcgacataat aatagctgta aagattcctg aagttcctaa acgttcgaca
     2461 aaagagccca tcctaatacc atttactaca gtttctgtct cattgataga agcaatcaag
     2521 ctgattttac catcagtcat gatcaattca gggatacaca taaagaatgc aaaaacagat
     2581 agtaatgccc ccgtgagcgg attaacattt aatttttgtt catctcgttc aattgacgtt
     2641 aattcataag caaacacaat ggcaaagtaa atagataaaa tccccatcgt cattgtattg
     2701 acgatcatgt acaagtcact aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat
     2761 gggaacggta atggcaaaat atttaaaacc aaaaacatcg agcctacaat cgtgaaagga
     2821 atagaagcca tccccgctgc catgacagcc cgaacgattt taagttgcgc aattttcccc
     2881 ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat
     2941 ataacgcttt ttcatattgt ttaatacgga tatactgttg gtcttggttg agtgaaattt
     3001 tctttaaacg atactgtata aatgcactgg caaaaatacc gcttacaaca agaatgaaaa
     3061 caaataactg tcctccattg ttatttgcca taaaaggata caagaatgta aatcctgagg
     3121 taaacaacac tggaatcact aataaattaa agacactctg gaacaaaaaa taccactttg
     3181 tccatggaac gttcgtttgg tgttttcgat aaagctttac ttgttctcct actgcaggca
     3241 aaatagctaa aatcagaatc atcggtaaac tacttcctaa acttttagct gatagaagca
     3301 gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt caggtaacgt acgattagat
     3361 aacgattgaa atacatgttt ttcaaactaa gttgcttttt ttctaattcg attttccttt
     3421 ttactgtatc ctgcacaatg atccttcctt tctatttatt tttttgatac aaataattca
     3481 tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag atgatcttgt gcatggacca
     3541 tgataatttc catttctatt tttgtcccgc tggcatattc tttcagaagt tgagtttgtg
     3601 actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa cttctcttct acttcttcaa
     3661 acatctcttt acgcatcgag cgaaatgctt catgtatcag tgtccgtgca tttccgctat
     3721 ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact gtttattttt tcagccatct
     3781 gttttcctcc ttattcgatc agtttgataa atttactgct gaaattctca aaaataggtt
     3841 cagctaagat ttcctgctgt acttttagct ggtcaactaa cctaacgatt gctttagtca
     3901 cgaccgtaat tccttcattt tcgacataag aaggcgaaac taaaaaaaca aaatgaattt
     3961 cttgatattg atcccactgc atcccagaag gaatcaatgc aacggctacc tttgtttcga
     4021 ttccaactgg aacagcggga tgtgggactg ccactgtatc actgaatatg atttgtccca
     4081 tattttcacg ttgctggatc tgctttcgca tttctcttat atatccttct tcttcattta
     4141 tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt tttcggttcg tacgtataag
     4201 tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa
     4261 atgctggtat cggtctatct gaggaatgat agggtataga ttctccaact acctttcgta
     4321 ttttacgaac atcatcttca ttcaaaaaaa tactgacatg taaaactgga attttgaaaa
     4381 acattgtcga aagatcaata gaagaaataa tcaggtcgat tccttttaaa gttgtttcat
     4441 tgatctcata atagcctttt acacttacca ctgtaatatt tttaccgaat tcactgacta
     4501 cgcgattctt taatagttgg gcactgccgt aacccgttgc acagataatc aatgcatgta
     4561 gtttatgcgc agctctttct ttttccaatg ccgccatgag atgcaaagcc aaataggccc
     4621 attcatcttc attgatggta tagtttttca aagccggcat gttgctgaaa tgccttttta
     4681 ctgcgtcaaa gactttggga ttttctttct ttatctcctc agtcaatggg ttctcaatgc
     4741 gaatacctcg gctcaatcga atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa
     4801 gctggagatc ctctactaat gaaaaatgaa attcctgaga caatcttttc agaactttcg
     4861 ctaattcctt tgatagttcc tgatcatttt gattttcttt gtgattcgac ttagccatga
     4921 gatgtaatgt caaataagca atttcttctt cgggaaacga tacacctgcg attacctcaa
     4981 tacgttgaac gatccgctgc gccacttgat actctactcg cttagaaact tcttgttcga
     5041 tgcctaattc cttgatttca aacccttctc ggagccgttt gatccccaaa gccaagtgta
     5101 aagtcagatt ctgtatgata aaatccgaaa cttttaactt agcttctctt gtttcatcga
     5161 gaatgataat gactagctct tcaaaactga tgtctgggaa aaaacggctt tgccctaaat
     5221 attctttcaa tgaatttcca taatggtttc caaaaaaagt atccatgata aaatgccgtc
     5281 gatttctttc ttccccttcg atccatgtcc cttgaccatg cttagaaacg atttttagtg
     5341 aatatggaat caatcgctcc ttgatttctt gcatgtcctt tgtcaaactg gaacgactga
     5401 tataaagctc ttctgccaaa tcatccataa aaagaatggc atcttccaaa agcaattggt
     5461 tcaatatata cttttgtcga tcttcggact cataaaatcc ttgcggttct ctagcatatc
     5521 caggtactat ttcttgccga ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca
     5581 gttggtagcc ataacctggt ttagcaatga tcttacctcc gtttttttct actaactctt
     5641 tgatccgctg caagtaatta cggattgtcc gatcagataa taacaattca gaagctaaac
     5701 gttgactggt gacatactcc ttcttgtgat caatcaaata tctcaacagc aatttttcct
     5761 tctgtttcat tatcatccac tcctattcca tcatatgttc tcttcgatca cctctgctaa
     5821 cttctggatg cctgtaggga ttgggacata cgcttggaaa gggatactaa caacaggctt
     5881 tccaacagat tctcctaatt ttttgaattt atccaaaaac atcgtcgttt gcgggctgat
     5941 cagaaataaa tcaaaatcac tattcttaat catattatcg ccttctgtag ctgaaactgc
     6001 atctacgagt acctcttctc ttttttgctt aaagaaatcc gttgtctttg aggccatcat
     6061 cgaggatgac attcctgccg cacaaataat caatgctttt ttcataaatc aatcactcct
     6121 tttattttta taaaccaagc atatcttgta agcgttttct attcaaaata tatatttccg
     6181 tagcattgcg gaaaaaatgt gtatcatatc ttgaataata atttctggca ccgtgatata
     6241 ctagtgatag aattttttca ggaggggatt tagtgagtat cacagtgaat attttgtata
     6301 caagtacaaa tggacaagcc agaaaatttg cagaagaaat ggtcgaaaga aacattgtca
     6361 aagcgattcg tgcagaagaa ggaaacgaga aatatgacta ttactttcca ctagaagatc
     6421 cagaatctct tttactgatc gaccgttgga aagatgaaga agcaatcgaa aagcatcaca
     6481 aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag
     6541 tcgaacgttt cactgattta ccgtaaaaag atacacatgg aatgatagca atttatcatc
     6601 catgtgtatc ttcacttttt ccttattttt tattcgatct catctaatga ttcaaaccgt
     6661 gtttttctct tatttttcgg ataagtattt cagtcacccc aatatacatg attcctaaac
     6721 ataccccaag gatattctca aaaatgcgga tcgttactgc tcctggaacc ccgaataaac
     6781 tggcagtgat tgccaaagca ccaaaagaat taaaaactgt tttatatcta taggtagaac
     6841 aaacacctaa agctagacca cctaatatac cgacccagtt aaaaggaata aattggctga
     6901 taccaataaa taaaacacat ccaatcaatg agccaatgat tcggtctact gctcgttctt
     6961 tgaaaccaat cgacatcaat ccatagctag atacaataga agaaaatgca aaccctgccc
     7021 acatcaatcg ctgaaaaggg acatactctc ctgcaacgat caaaagacta atgccaaatg
     7081 catagatgag catccaaatt ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa
     7141 acaagctttt gtctttgttc ttttctcgat gttttctgta aaggagaact gaaaaccaac
     7201 aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa attcttgttc aacgaatctt
     7261 taggtaacga ataaacaata aagagatatg aaaagccgta caaagaagca ttccccattt
     7321 tgggatcact agcggttgcc attaatatgc tgcttagtaa aataaaatga atcaacatgc
     7381 ccataactga ccatttgatc agctggacat acggtgctac taatagtgag aacatgacga
     7441 ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa gtcaacaaaa cgaatactca
     7501 tcaacataca gaataaaacc acagcgtatg gtttatgctt cgattcaaat aatgttgtaa
     7561 ttataatgat aaaacaaatg gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac
     7621 aaatatacat gcacttttct tttttagaag ctgcttctcg tattttttgt tttaaaataa
     7681 acggatctag ttgtagagac tgataaaaat ccacctgttg tcgtcctttc tttttacttt
     7741 tattttttac agtcatctat gacaggttgt aaatactatg gaacatcata tggcgttgtc
     7801 attttcttgt caactatttt tcctattcat gtttttataa aaaaacatat cgttttcttg
     7861 tatgattaaa tcaccacaaa ataaacaact gagagaacga tatgctttat gaatggttct
     7921 atcaaaaaaa ttctgagatc aatagacaaa aatttgatga ttacagaggt ctcttacgaa
     7981 accttacaga agaaaaaaac attgatcgtt gctgcagttc tcttccctgc tccatgtgtc
     8041 tgtagaaatt gtggttctgc tgtggtagat ggaaatagga aattaatggt ggtgaaaaac
     8101 aagaagaagg aaaccattgt ccactttgaa taatataatc atatgccaat ggttatacgc
     8161 ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat
     8221 tttatccgtc caaggcactc gatttcaaat catgtcagac ataaaatcac ttctttactg
     8281 actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt
     8341 attcgcacat tgaaagagtt gagaagctat ttaccaaagt cttccaaaag aattctgtct
     8401 tgagtgttga gggttgtgaa tttcgttctc acgtttccgt tgaggataaa ctgagtttta
     8461 tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc
     8521 tcgattaaca aactatttcc ttagctgcac taatcctaaa gaaatagaat tcttagttac
     8581 agacatgaac gccacctact ttcagctcac caaacatgtt ctaccaaatg cgaaattggt
     8641 gattgatcga ttacttggtt tttccacgcc tttcaaggag gcgtaacctt tattcacgag
     8701 ttggttgaag ctttccgaga caaaagaccc gacttgtttt tctccttatt gacaaagagc
     8761 cattaaaact agagaaaaaa ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa
     8821 gtattaccca ataaacttga gtcattaggc aaaaaacact gcgaaactca agaatgattt
     8881 tcgcagtgtt tttgtatatt ttttattaac tagtattact acaaaggttt atttttcact
     8941 tctcaacgca gcacaaaatt gtctttactt atttactggt tgggttcaac cttctctact
     9001 ccgatactaa tatttgtttg tatacccctt ctaaaatcgg tagtttccat tcaattgatg
     9061 ggtactagtt gattttaaaa actatgctta ttcaattttt tattttagct catatatttc
     9121 gtttctttct aaattttata ctgatgaaaa caagtagcac acccaatatt gacaatataa
     9181 actggctaat gatggtttct ccagtttttg gaagatgctt atttccactg cttgttggat
     9241 tgtttcctct tatatctgag ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg
     9301 ccacttcttg ttctttttcg atcttgaaag ttactggtgt cgcatccaat tcataccccg
     9361 ttggcgcttg agtttctacc agctgatatg ctcctggtgc taagccatct atcgccagtt
     9421 ttccatctgc tcctgtggtt agacctgact gcagaacggt tccattggca tcttgtaact
     9481 caaaaacagc tccttgcagg acttctccgc tttggtcatc gatctttgtc aacacgacac
     9541 cgcctggtgt aagtcgattt tctttcgtca attctaccac cgctgtctga cttctttcga
     9601 tctcaaactc gattggtgtc gcatccaatt cataccccgt tggcgcttgg gtttctacca
     9661 gctgatacac tcctggtgct aagccgtcaa tcgccagttt tccatctgct cctgtggtta
     9721 gacctgactg cagaacggtt ccattggcat cttgtaactc aaaaacagct ccttgcagga
     9781 cttccccgct ttggtcatcg atctttgtca acacgacacc gcctggtgta agtcggtttg
     9841 ctttcgtcaa ttctaccacc gctgtctgac ttctttc
//
LOCUS       DAZKNO010000102         9853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000102 DAZKNO010000000
VERSION     DAZKNO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.104"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(5..517)
                     /locus_tag="ACG8MY_001866"
     CDS             complement(5..517)
                     /locus_tag="ACG8MY_001866"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0431048.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(531..2828)
                     /gene="recJ"
                     /locus_tag="ACG8MY_001867"
     CDS             complement(531..2828)
                     /gene="recJ"
                     /locus_tag="ACG8MY_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0431049.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(2970..3413)
                     /locus_tag="ACG8MY_001868"
     CDS             complement(2970..3413)
                     /locus_tag="ACG8MY_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0431050.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3414..4202)
                     /locus_tag="ACG8MY_001869"
     CDS             complement(3414..4202)
                     /locus_tag="ACG8MY_001869"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0431051.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4199..5140)
                     /gene="rnz"
                     /locus_tag="ACG8MY_001870"
     CDS             complement(4199..5140)
                     /gene="rnz"
                     /locus_tag="ACG8MY_001870"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0431052.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5317..5904)
                     /locus_tag="ACG8MY_001871"
     CDS             complement(5317..5904)
                     /locus_tag="ACG8MY_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0431053.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6125..7438)
                     /gene="obgE"
                     /locus_tag="ACG8MY_001872"
     CDS             complement(6125..7438)
                     /gene="obgE"
                     /locus_tag="ACG8MY_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0431054.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7735..8745
                     /gene="gap"
                     /locus_tag="ACG8MY_001873"
     CDS             7735..8745
                     /gene="gap"
                     /locus_tag="ACG8MY_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0431055.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8830..9765)
                     /locus_tag="ACG8MY_001874"
     CDS             complement(8830..9765)
                     /locus_tag="ACG8MY_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0431056.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2707 a         2259 c         1704 g         3183 t
ORIGIN      
        1 ctgcttaata ttccattaat gtaatgatgt catatccttt gattttgtcg cgtccatgga
       61 gatccatcaa ttcgatcaaa aatgcgcagc ctacgactac accgcctaat tgttcgatca
      121 gttcgatcgt cgctttgatt gttcctccag tagctagcaa atcgtcacaa atcagcacac
      181 gttgacctgg tttaatggca tccttatgca atgtcaatgt atctgttcca tattctaatc
      241 cataagtcac ttcgatggtt tctcgaggta acttcccttt tttgcggact ggggcaaaac
      301 cgactcctaa ctcataagca acaggacatc ctacgatgaa tccacgggct tctggcccaa
      361 ctaccatatc aattcgtttt tcttttgcat aatttacgat ttgtttagta gcttcccgat
      421 aagcgtcacc atctgccatc agtggggaaa tatctcgaaa cacaattcct tttgacggat
      481 agtcgggaat actggcaata taatctttta aatccactct ttgtttcctc ctaggttgtt
      541 aaccatttct ttatggtagg aatatcgctc aaaagcaaaa attcctcaac tttgatttta
      601 ttgagccgtc gctggtataa tctgctctca gtcaaaggat gactttctgg gctagcgact
      661 ttttgtaaaa caccgttctc tattgtaaca aatttcaatt caaaaaacac ctgtatcata
      721 aaaattaata atttttcagg gatacgcaaa tattgagcta ttgcttttat tttatgacgt
      781 atatcaattc gttcttgaga atggatcaat ttaaacaatt ttgcgtactg ttctcttgtg
      841 cctaccccat tcaaataggc ctcatcaggt gaaatcccca tcagataaac acgattgaat
      901 gaataagacc gaaaaacttc tttcagatcc gctaaatcat ctggacaatc cataacaact
      961 agagagtttg gttttctttt ttcaattatt gcctttacag aatctagtcc ttcgaaaaca
     1021 atcgcagctt cctggataat ctcaggcacg aatttcaacg tcgaagaatc aaatgctaaa
     1081 taaaggctac cttccgacgt tcggctctgt tcacggaaac gttttgcccg ccaatcaaat
     1141 acttgcagac cacccacaga aaaatcagat accatcagct gcggtttctt acgcccgttc
     1201 cattcattga tcgaaagctt tcctaccaca tctacctgat tattcagcag ttcttcttct
     1261 tgagtaccaa aaccaaaagc gaccgcatca agctgactcg atgcatcgga aaggacaaat
     1321 ttcagatgct gttggtttgc tccgattctt ttgatatttt ctgcttggac ttgtcgaaat
     1381 aaaaactgag gcaaaggatt atccgtacca aatggcgcca atagtttcag ctcatcaatt
     1441 cgctctactg ttacttcatt tggcagcaat acttcatcaa ttctcaactc aggccctctc
     1501 atcaagtcaa tttgatgatc gactatatat tggttcatct tctcctgtag tatagtgaca
     1561 ttttcacttg gcatcgttaa accgactgct gcatgatgtc ctccgaagta agtaaataaa
     1621 tctcgcatct ggtcaagcat ttcaaaaaga ttcaaggctt caatactacg tccagatcct
     1681 ttagctgatc catcttcttt taaagtaagg acaagcgttg gtttacccgt ttcattcatt
     1741 attttcccag caacaatgcc caaaacgcct tcatgccatc ctcggtttac caatagatgg
     1801 atatgattct ccttattgac catcgctaat gcttcttgtg ttatctgctc tacgattgct
     1861 tttctttctt cattgatctc attcaatttt tttgcttgaa cggatgcttc ctcttcatca
     1921 aacgtcgcaa gtaacgagac ggcaggacgt ggatcgccca tccgaccgat tgcatttagt
     1981 ctgggagcaa tcgaaaaacc gatcgttgtt tcgtctgcgt ccctcatttt tacgccgctt
     2041 tcttcaaata gcgcctgaag tcctatccgt tctgaatgat gaatggcatc gattcctaat
     2101 gaaacaagga ttcgattctc atcagtcaat gaaactaaat cggcgatcgt tcctatcgct
     2161 actaaatcca aaaattctgc aggcggttct tccaataatg cagaagctac tttgaatgct
     2221 actccgactc ctgccaaatc tggaaatgga tagtgccccg ctggatgacg agggtgaaca
     2281 atcgcataag cgtctggtaa attctcaggt agttcgtgat gatctgtaat gatcacgtct
     2341 acaccagctt gctgagcgta tgctaccgca tcatttcctg ctaccccgtt atcaacagtg
     2401 atgatcaact ggattccttc ttcgatcttt tcttggtata ccgattggtt tggtccatac
     2461 ccatgctcaa atcgatttgg caggaatact tctacttctg caccaagtaa ttccagtgtt
     2521 tctttcatta ccgttgcact ggtgatccca tctgcgtcat aatcaccgta aacgaggatt
     2581 ttttctccac tgattacagc ttcttggatt cggctgattg ctttatccat atcgtgtatc
     2641 agatacggat catgtaattc ttgttccttt ggatgtaaaa atcggttgat atccttttcc
     2701 tcatggtaac ctctttgcca cagtaactta ccgacaaatg gagataattt cagttcacgg
     2761 atcatttgga gaaaggattc agaaggctct gtctcgctta acttccaatc aaaatttgct
     2821 tgtctcactt tctcactcct aacttagtta ttatagcaaa ggaaatcagc aaaaaaaagc
     2881 agaaagaaag aataaaaaca gatcagatcc gctagacaga tgttcagcag gacctgatct
     2941 tttgtacgac catttatgaa atatccctat tagtcaaaat aattgaagct ttgtttgggc
     3001 gattgagttt tttgttcttc cggcgctttc aattgactga tttgtgcggt tttcgcttgt
     3061 aactccgcct gataagcagc ttccaactcc gcacgttcgt ttctaaactc gcgttgctgt
     3121 tcttcttttg cttcttcaat tttcttttga agttctgttt gatattctga catttcctga
     3181 gtcagctgtt tgatttgctt tttttgctgc catagtgttg ccgaagcagt caaaaataca
     3241 atcagtgcac caatcagcgc agaaccgatg atcactaaga tcaatggcgc agaaaaggaa
     3301 gtgaagccaa aacttacagg aacattttta ttgttcaata cagcaaaaag aacaataatc
     3361 aaaactaaaa caaatccaag aatcacattg ctttgctttt tcaattcctt cacctacttt
     3421 ttgttaaaaa ttccgctggc taaataatca ccaatcgttg gaaacagcgt ataaaatttt
     3481 gatgcaactt ctaaaataaa cggttgattg atttcccgtt ttggatgtct catattgcgg
     3541 acgatcattt ttgccaactt ggcaggttct aaaacgattt gtccgacttt ttccaaatag
     3601 cttccgcttg gatcagcctt atcgaagaaa tttgtctcga ttggacctgg attcactgtc
     3661 gtaacagcta cgccaagtgg tttcaattct aatcgcaaag cgttggagaa gccaaggact
     3721 gcaaatttcg tggcagaata aaccgttgat tttgcagttg ccatttttcc agccatagag
     3781 gcgatattaa tgatatgacc ttgtcttctt tctgccatat cgatcgcaaa tttttgtgtc
     3841 aaaaccatca ttcctaagat gttgacttca aacatatcgt atgcttttcc aaggtcgaat
     3901 gtaaggaagt cttcgaaaat cccaaaaccc gcattgttta ccagaatatc gacgcgcccg
     3961 acttcttcca tcaccttttc atataattcg tccacgcttt caggattgct gacgtctacc
     4021 ttgaaagcat aagcctcttt gccgctcaag cgtctgcact cgtctctgac accttctaca
     4081 aatgccattc ttcgtgcgca acttataacg attgctcctt gctttgctgc ttcataacag
     4141 atttgtgcgc ccagaccgcc tgaactacct gttactaaaa caactttatc ttctaaattc
     4201 atgcctcttc ctcctcgcga aatggaatct caatgctatc aaaatctttc ataatcgtcg
     4261 tgttttcaaa cacttcttgt gcttcttttt caagctctaa agctgctttt cctaaatatc
     4321 tcgcactgat atgagtaagt agcagccgtt ttgcctgcgc ttcttttgca acttcagcag
     4381 cttgatgagt cgtggaatgg aaatagttgt gcgccattct tgcttcatct ttattaaatg
     4441 tgctttcatg taccagaata tcgctatttt ccgcaagcac aacggaatta tgtgtttttc
     4501 tcgtatcgcc taaaatagtc acgattcgtc cttttttatc tggacctacg aaatcttttc
     4561 cattgatcgt ccgcccatct tctagttgga tcgtttcacc ttgcttcagt ttgccgtaca
     4621 gtggtccagc tggaatacct aattctttta gacgatccac ctgcaattct cctttatgat
     4681 catgttcaac cacacgataa ccaaaactgt cgatcccatg atttagtttt tttgtataaa
     4741 cagaaaattg ctgatcagta aaaatcgggg ctgtttcatt tagctcaatg aatttcagcg
     4801 gataacttaa acgcgtttgc gaaataccta aagagacttt tatgtattcc tctatccctt
     4861 ttggcccgta aatctctaat ggcgtatccc caccttgaaa tgaccggctg ctgatgagcc
     4921 cgggaagtcc aaaaatatga tcgccgtgta aatgtgtaat gaaaatttta ccgatttttc
     4981 tcggacgaat agtcgttcgc aaaatctgca tttgcgtgcc ttcgccacag tcgaacaacc
     5041 aaacttcatt gcgctcgtct aaaagtttca atgctatact tgtcacattt ctatgttttg
     5101 caggaacacc tgcacctgtt cctaaaaatt gtaattccat aatctacctg actttcttta
     5161 agttcctctt taattttagg gtaaaaagaa gggataatca accgtaagtc gagaatgggt
     5221 tcaaattaaa aataacgttc taaagcgata gcgttttctt tgaaaattac tataaaaaca
     5281 gcagcgaacc cgtttgagat cctctgctgt tttttattaa tttgaagatc cagaactttc
     5341 attgtcgttg gaagtttgcg gatgtttgtc cttccaagtc ttttgtgcag cttctttcgc
     5401 atgattttca gcttctttcg catcatgcac atctttttcg acatcttcat aatccaagcg
     5461 agtaatttct ccgcctaatt ccatcattac caattgattc agtgggcgat tgtcctcttc
     5521 atcagcaatc aagatcagtc ctgtagctcc ttcaccgatt ttatttccta caaagtcgaa
     5581 cacggtctgg gcatctttta tttctttggc atcttgtgtc gcaccgatca ttcctccggc
     5641 aaaccatcct aataaaatcc ctagcggacc acctaataca ccaaccaaca ttccgattaa
     5701 tccacccgta gaagatttgt tattaccggt aaagtcgata aaatcttcta ttttgaattg
     5761 atgtgtccca ttaggatcat ggctgacaac tgccatctgt tctcctttaa tcgaacgaac
     5821 agcatgtaac tttttgattt ctgaaaatgc ctgatatgcc tggctttcta cttgaaaatt
     5881 taaaatgatt actcgtttgc tcattaaaaa cgcccctttc ataccttctt gttatttatt
     5941 ttacctttaa ttgtaaaata agaaaagaaa agcactcttt ttttccaata aaatagggct
     6001 gtgacaaaag tcgtatcaca gctccttttt ccgaataaac ggcgttcttg aagctgatca
     6061 cttctgtcac aacctccctt aaatctgaat ctatattttt tggtatttgt ttttatttgc
     6121 ttgcctattc gacaaattca aattcaaact caccgatacg tacaaggtcg ccatctttcg
     6181 caccgcgtgc ccgaagagct tcatccacac ccataccacg taattgacga gcaaaacgca
     6241 tcactgtttc atcatgatcg aaattcgtca tttggaataa tttttcgatc ttctcaccag
     6301 ataagatcca cgttgcatct gaatcacgat cgatttggaa ctcaggacct tctggattga
     6361 atccatagtg tacagtttct tcttccagtt cttcttcgta caatgggaat tctggcgtta
     6421 cttctaacaa atctgctgta gcattcaata acgcgtccaa tccttgtcga gtcactccag
     6481 aaattggaaa tactgggatg tcatcagcaa attcatcttc tttttccttg ttcagctgtt
     6541 ctttgaattt cactagattt tccggtgcat ccggcatatc cattttgttt gccacgatga
     6601 tttgcggacg ctctagcaag cgtaagttat aagtagataa ttctttgttg atggcaagat
     6661 agtcttcata aggatctcgt ccttccatcc cactcatatc gatcacatgt aaaattactc
     6721 gtgttctttc gatatgacga aggaattgtg tacccagccc aacaccttgc gaagctcctt
     6781 caatcaagcc tggcaagtct gctgcggcaa aacttctgcc atcacttgta gtaaccattc
     6841 ctagattagg tacgagcgtc gtgaaatgat aagcgccgat ttttggacga gcagatgaaa
     6901 taacggatag taaagtcgat ttcccgacag atggaaaacc tacaagtcca acgtctgcca
     6961 atactttcaa ttctagttcg atttttcgct cttgtcctgg ttcaccgttt tcagcaattt
     7021 ccggtgcagg attccgagga gaggcaaaac ggatattccc tcgtccaccg cgtccacctt
     7081 ttgccacaac tagtgtttga ccattttcaa ttaaatctcc taaaagagca ccagtctccg
     7141 catctcggac agtggttcct tgtggtactt taacataggt atcttctgaa ccacgaccgt
     7201 gcatcccttt actcatccca ttttcacctg gctgcgcttt gaaatggcga ttgaaccgaa
     7261 aatccatcaa tgtacgtaac ccttcatcga cgatcaagat gacatcgccg ccacgaccac
     7321 cgtctccgcc agccggtccg ccgtcaggaa cgtatttttc gcgacgaaat gcaaccattc
     7381 cgtctccgcc cttgccagct ttcacatcga tcgttacttg atctaaaaac atggacataa
     7441 ttaagtcctc ctattattca tttatttatt tgactgcctg aatttccaca ataaacaaaa
     7501 gtcttactta ttttatcggt gaaatctgat tttgtctatc atttcagcaa aaagattgtt
     7561 taccttcttc tttttttatc atttggtaag gtttccaacc ttttttctgc taccaagtat
     7621 aattttttga caaaatattc tttgcgtagc agaataacag tgtattaatt gtattaattg
     7681 tattaattga ggatgccgtt tcacagcacc cctaataaag gaggaacttt tgatatgaca
     7741 gtaaaagtag gtattaacgg atttgggcgt attggccgtc tggctttccg ccgcatcaaa
     7801 gaagtctcag atgatattga agtagtagca atcaacgact taacaagtcc aactatgtta
     7861 gcacaattat tgcaatttga ctctactcat ggtacttatc ctggcacagt tactgcaaca
     7921 gaagactcaa tcgtagtgga tggcgaagca actcgtgttt atgcagaacc cgacgcaagc
     7981 aaaatccctt gggcaaaaga aaacggtgta gatatcgtac tagaatgtac tggtttttat
     8041 acatctgaag aaaaagcaaa agcgcattta gatgctggtg tcaaacgagt agttatttct
     8101 gccccagctg gtgctatgaa aaccatcgtc tataacgtca atgatgatac attagatgaa
     8161 aacgacaaaa tcatctcagc aggttcatgt acaacaaact gtttagcacc tatggcttat
     8221 ttcttaaata atgaatttgg gatcgaagtt ggtacaatga caacagttca tgcctacact
     8281 tccacacaaa tgctcttaga tggacctgta aaaggcggta atttgcgtgc tgctcgttca
     8341 gctgctgaca atactatccc acattctact ggtgctgcga aagcaatcgg cttagttatc
     8401 cctgagctac aaggaaaatt acaaggacac gcacaacgtg ttccagtcgt tgatggttca
     8461 ttaactgaat tggtttcagt attgaaaaca aaagtaactg ctgatcaagt aaacgaagca
     8521 atcaaaaaac acacagttga caatccttca ttcgggtatg atgaccgtca aatcgtttct
     8581 agcgatgtca ttggaacaac tcaaggttcg atctttgacc caacgcaaac agaagtaaca
     8641 actgctggtg atttccaact tgttaaaact gttgcatggt atgacaatga gtatggattt
     8701 acttgccaaa tgattcgctt actagaaaaa ttcgctaact tgtaatttga tagatcaatc
     8761 gataaaagaa gcctttgact ggatccagtc aaaggcttct ttcttttcat cccctacttt
     8821 taaactcgtt caatgaacat attggcttaa aaattgttgg actacttgct gatatttttc
     8881 aggatctgtc ttgtatgctt ccgcatgctc ggctcctttt acaacatact tttcttttgg
     8941 tcccttcgtt gcacgataga ccttatctaa catttcaaat ggtacaaatg tatcttcgtc
     9001 tccatgtata aagagcattg ggcgctggtt tttcttcaat tgttccactg catttgcttc
     9061 accgaaaaag tagccagcac ggacttttgt cactagactc gttacttgca taagcggaaa
     9121 tgctggaaga ctaaacatat cgtctagctg ataagcaagc tcatcatgaa cggaagaata
     9181 accgcaatct tcaatgattg ctttcacatt atctggtagt ttttctccgc tagtcatcat
     9241 aactgttgct gctcccatgc tgacaccgta tagaacgatt tcttgcgatt ttccattgtt
     9301 ttctagcact ttgtttatcc actgtacata gtctttacgc tctggccatc caaatccaat
     9361 gtaatcccct tcgctttttc catgtcctct tgcatcagga acaaggacat tgtaacccat
     9421 atcatgaaac atctttgcgt aattggtcat cgtttcagcg tttcccatat agccatgagc
     9481 cacaagagct gttttttcac ttttcgtttc agcaggaaga taaatcgctg ataatttcag
     9541 accgtctttt gaagtgatgg tccaatcgct tcggttttct tttgaactaa accatttatg
     9601 tgcatccgtt tctttggatg tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt
     9661 ctcagaaggc acaacagcat aattataaaa ataattccct gcaaacacta acgcaacaat
     9721 cagagctaaa gcgacgccta aaaggatttt catccaaagt ttcataaatc atccatctca
     9781 tttctctttt ttcatataac cttgaattag tataatcaaa gaatctctac tatgcaatgt
     9841 tcatttttat aac
//
LOCUS       DAZKNO010000103         9751 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000103 DAZKNO010000000
VERSION     DAZKNO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.105"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(263..1246)
                     /locus_tag="ACG8MY_001875"
     CDS             complement(263..1246)
                     /locus_tag="ACG8MY_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0431057.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1251..1898)
                     /locus_tag="ACG8MY_001876"
     CDS             complement(1251..1898)
                     /locus_tag="ACG8MY_001876"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0431058.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1983..2615)
                     /locus_tag="ACG8MY_001877"
     CDS             complement(1983..2615)
                     /locus_tag="ACG8MY_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0431059.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2631..3182)
                     /gene="coaC"
                     /locus_tag="ACG8MY_001878"
     CDS             complement(2631..3182)
                     /gene="coaC"
                     /locus_tag="ACG8MY_001878"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0431060.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNTITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3179..3916)
                     /locus_tag="ACG8MY_001879"
     CDS             complement(3179..3916)
                     /locus_tag="ACG8MY_001879"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0431061.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3913..4902)
                     /locus_tag="ACG8MY_001880"
     CDS             complement(3913..4902)
                     /locus_tag="ACG8MY_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0431062.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5160..5702
                     /locus_tag="ACG8MY_001881"
     CDS             5160..5702
                     /locus_tag="ACG8MY_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0431063.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5719..6804
                     /locus_tag="ACG8MY_001882"
     CDS             5719..6804
                     /locus_tag="ACG8MY_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431064.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6816..7622
                     /locus_tag="ACG8MY_001883"
     CDS             6816..7622
                     /locus_tag="ACG8MY_001883"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0431065.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7623..8450
                     /locus_tag="ACG8MY_001884"
     CDS             7623..8450
                     /locus_tag="ACG8MY_001884"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0431066.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8447..9520
                     /locus_tag="ACG8MY_001885"
     CDS             8447..9520
                     /locus_tag="ACG8MY_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0431067.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         2935 a         1804 c         1996 g         3016 t
ORIGIN      
        1 cttcttatgt ttttaacttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt
       61 ttattcggaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
      121 cgtataaacg gcggaagcag aagaaattcc ttcggaaata agctgaaatt cacaaaaatt
      181 tgaaaagcaa ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc
      241 ctcttttttg atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt
      301 tgtttgcttc catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa
      361 tgattggccg aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg
      421 cgagctctgc tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac
      481 gtttcccaat caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa
      541 tcaagaccca acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc
      601 cgatgaaatc caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt
      661 gaaagtcttg ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga
      721 gtttttcttc tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt
      781 ggatcgctgc tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt
      841 gtaattttcc aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag
      901 cagcagaaac atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag
      961 catattcggc ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag
     1021 tggagatatc acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt
     1081 agggaggttt caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg
     1141 tatggacttt gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt
     1201 ctaatacatc tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc
     1261 tttgcccatt cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc
     1321 gtacgagaaa tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga
     1381 gtgatcgttt ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca
     1441 tcgttagtca atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat
     1501 tcatctgcat aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt
     1561 tcatctgcat aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg
     1621 atcactgttt tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg
     1681 gcagcaaaaa tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa
     1741 gttaaatcaa aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca
     1801 tctctcaccc ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg
     1861 ccaatttgtc cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca
     1921 aatctttcca ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac
     1981 tatcagttag taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca
     2041 gtcgcaagac ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata
     2101 gctgaataaa cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa
     2161 aagtagatca tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag
     2221 aataaagaaa cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa
     2281 taaggaacca ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt
     2341 ccatgttctg ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg
     2401 ataactgtgc tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt
     2461 cttggtccta aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt
     2521 ggaccgatag ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac
     2581 atcgcagtta atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt
     2641 ctttcattaa gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct
     2701 tttccaaaat ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca
     2761 gatctcaatg tttccagatt acgctgagtg atcgtgtttt gatacatatt cgtgttcatg
     2821 gcaggggcaa tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga
     2881 tcgtctgcaa tgccatttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga
     2941 aacaagtcag cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc
     3001 atgacgtctg tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa
     3061 tgtgtgctgc ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt
     3121 gtaagatcag cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc
     3181 attttgattc ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt
     3241 gagcttcttc cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga
     3301 tttccgtcaa atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg
     3361 cgactgccag aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg
     3421 cttttggctg ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca
     3481 caagatgctc ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg
     3541 caaaccaatt gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt
     3601 cttctgaaaa actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat
     3661 cgtagtgttc ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga
     3721 tcgtataaat cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt
     3781 aatcgattgt ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc
     3841 ctgtcgaatg atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg
     3901 tgattagaat cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc
     3961 agcaagtagt ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag
     4021 ttcatctgca atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg
     4081 tacattgaca gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt
     4141 cgcgtttagt ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat
     4201 caatggaaga agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg
     4261 ctgttttcct aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa
     4321 agcacctaga ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga
     4381 ctggaatcct agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc
     4441 agtaacgaca acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa
     4501 catagcagta aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg
     4561 tagaggaact aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt
     4621 gacgccaagc ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt
     4681 actttggacg acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac
     4741 ctgaccagac tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt
     4801 taccaagact tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa
     4861 aacaggttcc tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt
     4921 aaaattttct tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt
     4981 agacaaaaat taggtgaaaa ataaagaatc aaacaactag aatttattcc aaaagataaa
     5041 gaaatattca gcaaaaaaaa ttttacaaag ggttgccttt ttcaataaat ttcgtatact
     5101 aatacgagtg gtagtaaaca ggaagtttac tattagaaaa cttcgattcg gaggaaatta
     5161 tggaaattgg tgagaagtta agaaatttgc gcatccaaaa gaatttgaca caagaagaac
     5221 taggcgaacg cacagatttg tcaaaagggt atatttccca gttggaacga gatttaagtt
     5281 caccatcaat ggaaacattc ttttctattc ttgaagtctt aggtgtgact ccagaagaat
     5341 tttttcatca agaagcagcg aatctgcaag tggtttattc aaaagaagat catacggttt
     5401 atttcgatga agaaaacggg tatgaattgg aatggctcgt ggctgattcg aatgaaaaag
     5461 aaatggagcc tgtcttgctg acttttgaag gtgcaggaga atataagacg tttgaacctt
     5521 cattatctga aacgtttatt tttgtattag aaggggaatt agagttaagc ttaggagaga
     5581 cggtctatgt ggcaaaagca ggacagtcga tctattatca agcgacaaaa caacatcaat
     5641 taaggaatca tctcaaaaaa cgaacaaaag tattgattgt tgcaacgcaa tcatatttgt
     5701 aatatagggg gtaaaaacgt ggggaaacca atcatttcat ttgatcaagt agtgaaacgc
     5761 tatgatgatg aaacagtatt aaaaaaagta agttttgaga tcgaacaagg aaaattttat
     5821 acactgcttg gtccttccgg ctgtggtaaa acaacgatcc tacgtattat agctggattc
     5881 accgaagcta cagaagggga catctatttt gaagggaaac ggttgaatga tttgccagct
     5941 aataagcgac aagtaaatac agtcttccaa gattatgcac tttttcctca tatgaatgtt
     6001 tttgataacg ttgcttttgg attgaagatc aagaaaatgt ccaaatcaga tatcgaaaag
     6061 aaggtaaaag atgctttgcg tatggtccag cttcctggat acgaaaaacg ggagattagt
     6121 gaaatgtctg gtggtcaaag acagcgtgta gcgattgcac gagcaattgt aaatgaaccg
     6181 aaagtattgc tattagatga accgttatcg gcgcttgatt tgaaactacg tacagatatg
     6241 cagtatgaac tgcgggatct gcaacaacga ttaggaatca cgtttatttt tgttactcat
     6301 gaccaagaag aagcgttggc aatgagtgat gagatttttg tcatgaataa agggaaaatc
     6361 gtccaaagtg gtacacccgt agatatttat gatgagccaa tcaatcattt cgttgctgat
     6421 tttgttgggg agagcaatat cgtggacggt gtaatgatcg aagataatct agttgagttt
     6481 gttggtaagc aatttgaatg tgtcgatggc gggatgcgtc taaatgaacc agttgaagtt
     6541 gttttacgcc cagaggattt gacgatcacg acaccagata aaggaaaact agtcgtaacg
     6601 gtcgatactc agctgtttcg tggtgtccat tatgaaatca tctgttatga tgaacagcaa
     6661 aatgaatgga tggtccattc aacgaaaaaa gctaaagaag gaagcaaagt aggattggcc
     6721 tttgagccag aagatatcca tgtcatgcgt ttcaacgagt ctgaagaaga attcgatgca
     6781 cgtcttgaca gctacgaaga atagggggga caagaatgaa aacaatgcgc aggatctatt
     6841 ccgtccctta tattttgtgg ctcggtttat tcgtgatcgc gcctgtatta atgatcattt
     6901 atcaatcttt ttttgatatg aatgggcaat tcacgctgaa taattatcta tcttatttta
     6961 cgtcggggac atcgctttct atgacgatca attctgtatg gtatgcattt ttgatcacgt
     7021 ttttcacgct gatcatcagt tacccgactg cttatttttt aacaaaactg aagcacaagc
     7081 aattatggct gatgctgatt attttgccta catgggtgaa tctgctgctt aaagcttacg
     7141 catttattgg gattttcagt gtgcacggca gcgtgaatca tttctttgat tttatcggga
     7201 tcgggccgaa acagatatta ttcacagact tcagttttct gttcgtggca acgtatatcg
     7261 agattccatt tatgattatg ccaattttca atgcattgga agagatgaat ccttctttga
     7321 tcagtgctag tagagattta ggcgcgaata gctttgagac cttccgacga gtgatttttc
     7381 cgttatcatt aaatggtgtg aaaagtggtg tccaagcagt atttattcct tctctttctt
     7441 tattcatgct gactcgtctg attggcggaa atcgggtgat cactttagga acggctatcg
     7501 aacagcattt cttggttaca caaaactggg ggatgggttc tacaatcggt gtgattttaa
     7561 tcattgctat gttcatcgtg atgctcctga caggcgagaa gaaaaaagga ggtcgctcat
     7621 aaatgaaaaa atttaaatgg tcgtatcttt atttagggat cgtcttttta ttcttatatg
     7681 caccgatctt ctatctgatt ttttattcct tcaatgaagg aggaacgatg aacagcttta
     7741 caggatttac ttgggaaaat tatgaagctg tttttgaaga tacacgtttg atcattatcg
     7801 tattgaatac gttcatgctg gctttcttat ctgcattgat cgccacgatc atcgggacat
     7861 ttggggctat gggaatctat tacacgaaaa aacgacgtgc acggacagcg ttgttgagca
     7921 tgaataacat cttattagtc tctcctgatg ttattatcgg tgctagtttc ttgattttct
     7981 ttacactact aggattcgga ttaggttttc agagtgtatt gctttcccat attgctttca
     8041 gcattccaat cgtcgtcttg atggtcttgc caaaattgca ggaaatgaac gattcaatgg
     8101 tcgatgcggc tagagacctt ggtgctaata atttccaagt agtcaaaaat atcattttgc
     8161 cttatctttc tccaggaatc atagctggat attttatggc atttacctat tctcttgatg
     8221 attttgcggt tacatttttc gtcacaggaa atggctttac gacgctctca gtagagatct
     8281 attctcgtgc aagacaagga atcagtttag aaatcaatgc gctaagtgcg ttagttttct
     8341 tgttctccat gatattggtc attggatact actttattag taaagaaaac cgtccaaaac
     8401 ggctgaagaa aaacagaaga gtacgcgagg gggtggcacg gctgcgatga aaaaattaca
     8461 atctctgttt ttaggtattc tcatcattat tgcggtgctg ctgcttggtg ttcgtcaact
     8521 tgaaaaaaca agtggcatga gtggagcaaa ggtacttacc atctataact ggggagatta
     8581 tatcgatcct agtttgatca cgaaatttga aaaagaatat ggatacaaag tgaattatga
     8641 aacatttgat tctaatgaag cgatgttcac aaaaatccag caaggtggga caaactacga
     8701 catcgctata ccaagtgaat atatgatcca aaaaatgatc aaagaaaaac tagtacttcc
     8761 attagatcat tcgaagatca aaggattaaa taatattgat ccacgatttt tagacttgga
     8821 ttttgatcca gataatcagt attcgattcc atatttttgg ggaactttag ggatcgtata
     8881 taacgataaa ttctttgatg caaatcaaat taagcattgg gatgatttgt ggaagccgga
     8941 attaaaagac agtttgatgc tgattgacgg tgcacgagaa gttatggggc tttctttgaa
     9001 tagtttaggc tattctttga atagtaagaa tatggcacag ctgacagaag cagcaaataa
     9061 attgaatcat ttaacaccaa atgtcaaagc aatcgtggca gatgagatca aaatgtatat
     9121 gatcaatgaa gaagcctccg tggcagtgac attttctggc gaagctgccg atatgatgtg
     9181 ggaaaatgaa catttacatt atgtgatccc accagaagga tccaatcttt ggtttgataa
     9241 tatcgttatg ccaaaaaccg tgaaaaataa agaaggtgcc tatgatttca tcaactttat
     9301 gctagaaccg gaaaatgcgg cacaaaatgc agagtatatt ggttattcta caccaaatga
     9361 agaagcaaaa gcgttattgc caaaagacat ttcgtcggac gaacaattct atccatctga
     9421 tgacacgatc tcccatcttg aagtctacaa agatctagga tcgaaatacc taggcatcta
     9481 taatgaccta ttcttagaat tcaagatgta taggagataa tggcaaaact cagtaagaat
     9541 ggggacttat caacgttcta aagagctaaa aatcgtcctt tttctatctg agttttccaa
     9601 tcttctacca aaagcggatg aaacgccgct ttttttgtgt tattttattt tttctaccac
     9661 gtttttctac cagagaaaag tggacaaaac cattgtaaaa tagggcccga gacgggctag
     9721 taaatctaag attgaaaaag ttacagaagg a
//
LOCUS       DAZKNO010000104         9645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000104 DAZKNO010000000
VERSION     DAZKNO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.106"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..657
                     /locus_tag="ACG8MY_001886"
     CDS             34..657
                     /locus_tag="ACG8MY_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431068.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8MY_001887"
     CDS             1107..2156
                     /gene="trpX"
                     /locus_tag="ACG8MY_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0431069.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="ACG8MY_001888"
     CDS             2153..3034
                     /locus_tag="ACG8MY_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0431070.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="ACG8MY_001889"
     CDS             3037..3807
                     /locus_tag="ACG8MY_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431071.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8MY_001890"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="ACG8MY_001890"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0431072.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="ACG8MY_001891"
     CDS             5118..6281
                     /locus_tag="ACG8MY_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0431073.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="ACG8MY_001892"
     CDS             6398..6808
                     /locus_tag="ACG8MY_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0431074.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="ACG8MY_001893"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="ACG8MY_001893"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0431075.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..>9645
                     /locus_tag="ACG8MY_001894"
     CDS             9381..>9645
                     /locus_tag="ACG8MY_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431076.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQV"
BASE COUNT         3350 a         1637 c         2076 g         2582 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtat
//
LOCUS       DAZKNO010000105         9623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000105 DAZKNO010000000
VERSION     DAZKNO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.107"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..2428
                     /gene="leuS"
                     /locus_tag="ACG8MY_001895"
     CDS             14..2428
                     /gene="leuS"
                     /locus_tag="ACG8MY_001895"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0431077.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            2776..4329
                     /locus_tag="ACG8MY_001896"
     CDS             2776..4329
                     /locus_tag="ACG8MY_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0431078.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(4710..6494)
                     /locus_tag="ACG8MY_001897"
     CDS             complement(4710..6494)
                     /locus_tag="ACG8MY_001897"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431079.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(6509..8230)
                     /locus_tag="ACG8MY_001898"
     CDS             complement(6509..8230)
                     /locus_tag="ACG8MY_001898"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431080.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            8330..9091
                     /locus_tag="ACG8MY_001899"
     CDS             8330..9091
                     /locus_tag="ACG8MY_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0431081.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            complement(9433..>9623)
                     /locus_tag="ACG8MY_001900"
     CDS             complement(9433..>9623)
                     /locus_tag="ACG8MY_001900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431082.1"
                     /translation="MLEEKSLNTYNYFISKKMLSVVSPIAEESHSRDYLIKKYVYSIH
                     LFIFALLTYCNNVSNNVF"
BASE COUNT         3117 a         1713 c         1927 g         2866 t
ORIGIN      
        1 aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc aaaaatactg
       61 ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat tttacgcact
      121 ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg aaggttatac
      181 ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc tgcatccaat
      241 ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag gaaacgatcc
      301 agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt ctttaggatt
      361 tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata aatggacaca
      421 atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag tagcggtaaa
      481 ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg ggaaaagtga
      541 acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga aaatcactgc
      601 ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa gcattaaaga
      661 aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta aagtggctgg
      721 tacggataaa tcctataccg tatttacgac acgaccagat actctattcg gtgcaactta
      781 ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg aacaaaaagc
      841 tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga gaacggatct
      901 tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag ttaatggcaa
      961 ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag gagccatcat
     1021 ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg gactagaaat
     1081 tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag atggtacgca
     1141 catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata aaatgaatga
     1201 atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac gtgactggct
     1261 attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg aggacggaac
     1321 agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa aaaacatcaa
     1381 gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca atgtcgttga
     1441 tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat gggcaggaag
     1501 ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag cagattatga
     1561 aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac atgcggtact
     1621 tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg ttgttcctac
     1681 aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa acaacgaaaa
     1741 aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa cttacggtgc
     1801 agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga ttgcttggag
     1861 cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt tgatcgttga
     1921 tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc ttgataaagt
     1981 gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt tcaatacagc
     2041 aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc tgccttatga
     2101 atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg gtgaagaact
     2161 atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa catacgatga
     2221 agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta aagttcgtgc
     2281 gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag cattagccaa
     2341 tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg cggtaccaaa
     2401 caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac tgatgagttg
     2461 tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt tgttatttca
     2521 aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac acgttcaatg
     2581 tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt ttgtctatta
     2641 atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc aaattcataa
     2701 attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa tgaagtatga
     2761 gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc agctaaacaa
     2821 aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct tttaagctta
     2881 gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg ccatatgagt
     2941 gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca cttttcgtgg
     3001 ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat ttggctccgt
     3061 taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca acagttttta
     3121 cataataaac caacttctca agatgatgac tatctttatt ttgagaagac ttcgacgagt
     3181 caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact tgatgattta
     3241 cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca tattttttta
     3301 ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata cctgacaact
     3361 aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac aatctattat
     3421 gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca catgacaatg
     3481 aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc tgatcctaag
     3541 caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac agagacgcac
     3601 tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat tgattcgcaa
     3661 tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg ttattggatg
     3721 gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc tggggatgat
     3781 gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat tatcacccaa
     3841 tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg tctatttatc
     3901 tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac tatggatggc
     3961 ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca aaatgtcagc
     4021 ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta tacaaacaac
     4081 ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca gacagggggg
     4141 atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac gttagagaca
     4201 aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc ctgcctcttc
     4261 cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca agtagaacaa
     4321 ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca agaaacaaat
     4381 tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga gttgacaaga
     4441 gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt gtcttattgc
     4501 cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca agtcttttgt
     4561 cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt attattcttt
     4621 gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga ctaatcatac
     4681 atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact attataaaga
     4741 ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc gacgatatcg
     4801 ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg gtgtgcaatc
     4861 acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa tgcttctgtt
     4921 cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt cgctagaatg
     4981 actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt ttcgctagaa
     5041 atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg tgcgatttca
     5101 gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat attatcagct
     5161 accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg acgtagttct
     5221 ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt attcagttca
     5281 taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg accaacgatc
     5341 gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat tttatctgat
     5401 gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc taaaagtctt
     5461 gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat cctatcgatt
     5521 gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat tggttcagaa
     5581 atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc acctgataga
     5641 acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat gaaacgaatg
     5701 gccggataga tagcacagtt cataaattga gcacgtttaa aagcgttgta ttgcttttta
     5761 tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt gaccactaga
     5821 ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata ctgattctgt
     5881 tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact gccaacgatc
     5941 agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag accagcagca
     6001 gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac ttgagaaagt
     6061 tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt gtcaaaaaac
     6121 gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa tgtgatcttt
     6181 tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct taagactgaa
     6241 cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact gaaagaaagc
     6301 tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat tcccatgatc
     6361 aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa tccgtaaaaa
     6421 tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt gttcaacgtt
     6481 tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca aactcctgat
     6541 aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt tttccttcat
     6601 ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca acaatcacga
     6661 gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct gttttatagt
     6721 ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc aacgctcgag
     6781 caatacatat ccgctgtttc tggccccctg aataattaga accgccttga gcaacaaagc
     6841 tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc ttgatcgctt
     6901 tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt aaaattgtcc
     6961 cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc tgttcttgag
     7021 tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg tcactaagtc
     7081 tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg atcgctgttg
     7141 tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag atttcagtat
     7201 atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt ggttcagcta
     7261 ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct tctatccgtc
     7321 gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc atcaagtaag
     7381 caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt aatgctcctt
     7441 ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc atgagcaaaa
     7501 aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga ttagcatagt
     7561 cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag gcacggatca
     7621 tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt tttggctgga
     7681 tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat acataaaaaa
     7741 caacagagat caatgtaacc catgttagta atagagaata acgaaacatc atgagaatgg
     7801 tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca atcatttgct
     7861 ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta aaacgactga
     7921 tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt tggaatccaa
     7981 acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt gttcctagaa
     8041 acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg ccacttgcta
     8101 tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg gcgatttcta
     8161 aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag aatgttttta
     8221 agatagacat attattcctc cttttttcct gatacttttc gattctatac cttcaagtaa
     8281 cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa tgaaaaaaat
     8341 gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga tccgctattg
     8401 ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc gattgtttga
     8461 tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag atataccgat
     8521 caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga cacttgatga
     8581 aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa aagagataag
     8641 ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat ttcctgtaga
     8701 agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg aaatcaaaaa
     8761 atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca gttcacagac
     8821 gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag acgtgatttg
     8881 ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga tgaaccatgc
     8941 ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat ggaaaacagg
     9001 agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca gaactgaata
     9061 ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata actcgaaaat
     9121 gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt aatgtggggg
     9181 attattagtc gtggatagat agaattttag ttttttagag gaaatatgtc tttaaaaatg
     9241 gaatggttgc aattcttagc gatatatacg atgtaattgt cctcttaaaa aagtatggaa
     9301 aaataattgt attgggtgtt atgtataatt catggtgaac tgagactaat gagaagaaaa
     9361 attcatattg ttatgagtga gattgtatca gatgagaatg ataaattaaa taggtgcata
     9421 aaagcattac acttaaaata cattgttgct tacattatta caatatgtta ataaggcaaa
     9481 aataaataga tggattgaat aaacatattt tttaatcaaa tagtccctac tgtgactttc
     9541 ctcagctatc ggtgatacta ccgatagcat ttttttgctt ataaaataat tataagtatt
     9601 tagagatttt tcttctaaca ttc
//
LOCUS       DAZKNO010000106         9564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000106 DAZKNO010000000
VERSION     DAZKNO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.108"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..1521
                     /locus_tag="ACG8MY_001901"
     CDS             94..1521
                     /locus_tag="ACG8MY_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431083.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1613..1978)
                     /locus_tag="ACG8MY_001902"
     CDS             complement(1613..1978)
                     /locus_tag="ACG8MY_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0431084.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1975..2340)
                     /locus_tag="ACG8MY_001903"
     CDS             complement(1975..2340)
                     /locus_tag="ACG8MY_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0431085.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2532..2780)
                     /locus_tag="ACG8MY_001904"
     CDS             complement(2532..2780)
                     /locus_tag="ACG8MY_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431086.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2779..2907
                     /locus_tag="ACG8MY_001905"
     CDS             2779..2907
                     /locus_tag="ACG8MY_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0431087.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2964..4658
                     /locus_tag="ACG8MY_001906"
     CDS             2964..4658
                     /locus_tag="ACG8MY_001906"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0431088.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4758..4931
                     /locus_tag="ACG8MY_001907"
     CDS             4758..4931
                     /locus_tag="ACG8MY_001907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431089.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKVV"
     gene            5309..6442
                     /locus_tag="ACG8MY_001908"
     CDS             5309..6442
                     /locus_tag="ACG8MY_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0431090.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6473..6793
                     /locus_tag="ACG8MY_001909"
     CDS             6473..6793
                     /locus_tag="ACG8MY_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0431091.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6822..7286)
                     /locus_tag="ACG8MY_001910"
     CDS             complement(6822..7286)
                     /locus_tag="ACG8MY_001910"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0431092.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7415..8664)
                     /locus_tag="ACG8MY_001911"
                     /pseudo
     CDS             complement(7415..8664)
                     /locus_tag="ACG8MY_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8693..9010)
                     /locus_tag="ACG8MY_001912"
     CDS             complement(8693..9010)
                     /locus_tag="ACG8MY_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0431093.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9036..9356)
                     /locus_tag="ACG8MY_001913"
     CDS             complement(9036..9356)
                     /locus_tag="ACG8MY_001913"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0431094.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         3096 a         1874 c         1706 g         2888 t
ORIGIN      
        1 tgctttttca gaaattaact gatgatttca aaaagaaaac atgtcataat atttcccata
       61 ttgtgcaaaa aaatacaagg cggaggctct actatgattg aagaatatat tgagaaaaat
      121 attttaagac agctgttttt atgtgggcaa ttctatgtaa ataaagaagt caatctagaa
      181 aagctctcca atctcctgca tgtttgcaaa accactttac tcaatgatat caataacata
      241 aaaaaagaat ttgaagaaca gatcgcttat acacatcggg aaaaggaccg ttacacttta
      301 tatttttcag aacatatacc tcgatgcaag atcatgcagc aattgtctca aaattcgttg
      361 tttttaaaaa cttgtctgct ttatttggaa gaagatgaac ctgattatct tcaattaact
      421 gaatgtgaat tcatctctgt cagcaaagct tattcattaa aaaaacaagt attagcttat
      481 tttaatgact gcgggatcga aatcgaccgc tactcccctc gattcactga aatggaaaga
      541 cgtctgctgc ttcttaatgt gtcgtatcgt ttaggcggtt ttaattcttg ggagctacct
      601 gaatcgtttt ttgaacgtgc cgatcgtttc attgaatccg taacggaaaa tagtggcaga
      661 ttttatgata aagaaaataa agaaatctta tcgattggtt ttgctatcag tttcctacgc
      721 cagcaagtgt gtgctgtgac aatagattca aaatttatcg aagaaatcaa aaaacgtcct
      781 atttacaatt atgtcgaatc agcatgggaa aacactgatt tccaaaccta ttataaaaaa
      841 gaagaattcg cctttatttt gacactattc aatctttgta attatggttt ccattcttat
      901 cagttgattg cggaagactt tcaacagcta caccaagtgt ttattgacaa tactcccgaa
      961 ataaaagaac tagttgctac ctttgaatct cacttcaatc aagaattatt cggaaaccaa
     1021 ccgttcgaac gtgcgctgat tcatctgatg cgttcggctt gggataacta tcaattattc
     1081 atgccagaaa aattttatct tttaaatgag gaacagacaa atttatataa agaagtccag
     1141 acaatttttt ccagctggtc cagccagtta ccttatgatc tacggttgaa tcctaattgc
     1201 atgcgggctt ttgtgatcga attgagcgga attctccgtc tgacaaaaga acatctgact
     1261 atctatatcg tcacaaattc ggatgttcat tatctgattt atcgggaagc cttggaagct
     1321 gtaactactt tcgattttca agttgcacca acaatttatt caagtatatc tgatataaag
     1381 aagtatgcgc agcagtcctc aaaccgggta ttatgtgaac gtacattata tacaccagat
     1441 gcggtccaat atgaaaatat tattcctatt tcgatcaata cgatcgatcg agctatcatt
     1501 tcagctgtgc aaaacaaata aggggctgtg acaaaaatca tgtcacagcc tcttatttgt
     1561 tgaagatgcc catttctgta acaatctctt gatacacatc aggctcacaa cctcacttat
     1621 tttggacatc tacgggtccg atctctttat ttaaaaaata agaaatgatg gttcggataa
     1681 ctaccaacgc cgctagttgg aaaatatcct gcaaggtagg tttgataatc gattcgatga
     1741 tcccagctga aacaagaatt tccaaactta gaagtatata gcctcccaac atctttttaa
     1801 tagcattgtt ctcgctgact cggtcgcttt gcacttttgt atggacaaat aaatatttca
     1861 aaaaatcctt gattgataga caaacacccc aaaccaataa acagatagca aagatatcaa
     1921 gtcccagtac aagtttttca aaatatggaa gaagaaactg catcaactct gtcgtcatcc
     1981 tacctctcct cttctttgtt tttctttgtc ttctatttcc tgattcaaga aataagaaat
     2041 gactgttcga atgataataa taagtgcaag cttcaaaata tctgtccaag tcggcttgat
     2101 aactgactcg atgatatcag caacgactag tacttccaag cttaacaaaa tataacttgc
     2161 tagcagtttt cttgtatctg cattacgagt attacgttct gcatagtttt tcttgacatg
     2221 agaccaatca aaaagtttga atagaacaag aacgatcccg acaattaaga taccgataga
     2281 acaaatattt aaaatagcaa tgatccattc tagaaattca attaaatttg agcgaaacat
     2341 atacgctacc tactttcttt taatttgttc ctattctttt attgtaaaag aaagttacct
     2401 tgttcgtcta tttaaaagtt gttctaatga taacggttcg cttaaatttt tctttttcac
     2461 tctccccttt ttctccctcc atccataaat cgtggaagta tccaaaacaa cgtagcaagt
     2521 taaaataagg cttagtccgt catcagcttt tcaaagaagc actgtctcgg tgcttttttt
     2581 gtacccaaat ttctaaaaat agaaccatac acctcctcaa atcttttctt cttacctacc
     2641 ctatttttat ttacaggaaa atcagacact tcttcttttt tgtcaaaatt tcctttttct
     2701 tctcgtccta tcatttggca ttctaataaa aacgaactat ccttggatat agaaagagaa
     2761 aacaaggagg cgtttttcat gagtaaaaaa ttaatcaaag tcggcatcgg tttaggtttg
     2821 cttgcccttg gcgctgcata tctaggaaaa aagacaggac tattcgaaga tgacagtcat
     2881 ttatatgatg aattcgaatc aatctagcat cctatttagt tgaatcacta cggacatact
     2941 tggaagacat tggaggaatc acaatgagaa aaagtaaagc aataatgatt ggtgccggta
     3001 ttgccaatat ggcagcggct gtttatttga ttcaagaagg caaatggaaa ggggatcaga
     3061 tcacctttta ttcattggat gatcacggtt caaatgatgg tgcaccgacc gacactgtag
     3121 tggatgaata ttggaataaa aaccatccat tagaaaacca aaaaggctat attgctcgag
     3181 gcggtcgcat gcttaattac cgtacctatg tagatttgat ggatctgtta agtcgaatac
     3241 cttctgttac tgagccaggg atgactgctg aagaagatac gagagatttt gatgcaaaac
     3301 accgaacctt tgacaaagca cgtttgatgg aaggtggcaa aggaatcatt gatggcggaa
     3361 aactgggatt caataataaa gaccgtattc ttctgactaa actgattgct atgcctgatt
     3421 cagaagaaga aaaactagac aatatcacta tcgaagaata tttcaaagat gatccacact
     3481 tttttgaaac aaacttctgg tttatgtggg aaacaacatt tgctttcaga acacgtagtt
     3541 ctgctcaaga attgcgtcgc tatatgcatc aaatgattta tgaattcact caaatcgaac
     3601 atctagtggg cgtcaatcga acacgttaca atcaatacga aagtattatg cttcctttga
     3661 tcgagtactt gaaagaacaa ggatgcaaaa tcattttgaa ccgccgtgtc actgactggg
     3721 aattcaaaga aacaccgatg caagatgaaa tcactgtgac tggtttgaaa atgaccaata
     3781 cactgactca agaagaagaa cacgttgtgg tcgatgaaga tacagcagtt attttcacta
     3841 atgggtccat tacagattca gctactttag gagattacga aacaccagct gtagaaaata
     3901 tggattatgg tgcagcttct agtctctgga aaaaagccag tgaacgtttc tacaacttgg
     3961 gaaatcctga caaattcttt gctgatcgag atgcaagcga atgggtcagc tttaccctga
     4021 cgacgaaaaa ccatttgttg ttgaatgaaa tcacccgtat cacgactcaa gaaccaggaa
     4081 atgccttgaa ttcatttatt tcaaccacac cgatcacgcc gttaggccaa aaagatgtca
     4141 atatgtctat cgtcgtgcat catcaaccgc attttacgtc tcagaaacca aatgaaacag
     4201 ttatttgggg ttatttcctt tatccaagac gacgtggcga atttgtggat aaacagtata
     4261 ttaaaatgaa tggaaaagaa atgctagaag aactgattgg acaactttca aaagtagatc
     4321 ctggaccagt gaatattcgt gaaaaagaaa cagaaatttt cgacagcgta ataaataata
     4381 tccctgtcta tatgccttat gcttccgcgt tgttcaataa tcgtgccaaa agcgatcgac
     4441 caaaagtcat tcctaaacat tcgaccaatc tggcctttac aggagaattt gtcgagcagc
     4501 cttatcagat gatctttact gaacaaagtg ctgtacgttc tggagaaatc gctgctttcc
     4561 attttgcggg tatccctatg tcacgtcttg tcaaaacacc gcgttacgac aaagatattc
     4621 ctactctagc tcgtgccgca aaaaaaatgt ttgaataatc aagaggttgt gaaaaagctg
     4681 ccctgcatca agtaataaga acaaccaggc taaaacagct tcttatgttt ttgatttggt
     4741 tggacttatt accgcagatg caagcttttg aacaccgttt accaagaggt tgtgaaaaag
     4801 ctgccctgca tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt
     4861 ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa
     4921 aagtcgttta gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc
     4981 ataaatttga cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg
     5041 tctgtttctt tttgaaaaca accatttttt caacgtttac tctcttgcca atccaacatc
     5101 atctaacaga tacaactatt tcaatgatta ccattacact aaaaaatcgg aaactcacct
     5161 ctccgaacac aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt
     5221 atcaaaaagg taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa
     5281 ttttatgaat ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa
     5341 aatggaaatc agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg
     5401 tatcacagca acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt
     5461 tttggaaaaa gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc
     5521 tcaagttcaa acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg
     5581 tcattgtcat ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca
     5641 gttatatggc gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc
     5701 ggaatatcta agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct
     5761 tcctgatgaa aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga
     5821 aaacgcagga gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct
     5881 attcgctcaa aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt
     5941 acagcaccgt ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga
     6001 cacggcacaa gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat
     6061 cgattgtgta ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag
     6121 cttacaaaac ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg
     6181 aacagtccaa gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt
     6241 ttttatgcgg gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa
     6301 aatttacgaa atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct
     6361 gccactatca gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat
     6421 aaaagttgtt ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa
     6481 aatcaaacga aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg
     6541 acgtcgcttc cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt
     6601 attaaccggg attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg
     6661 atatggcacc tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag
     6721 aatcgtaagg aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa
     6781 aaaagaggca tgagatttga aaaaatctca tgcctctttt ttcaccctat cgtatcactt
     6841 tgatacagac gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg
     6901 tatcgttcat aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga
     6961 tgcatttctt ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc
     7021 ggttcaacaa acagggcaca aagatttggt gtcaaatcag ggcggttatg gaagtcgttt
     7081 tcaataacgc ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga
     7141 ggaatacttt ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga
     7201 actccccatt ttgatgaaaa ccacttcgca ctttgtttta ctaattctgg gtgttctctt
     7261 aatttgatcc agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca
     7321 aaaattatac aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaacg
     7381 gaaaacaatc acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc
     7441 tttcttggat cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat
     7501 tgacagcggc tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg
     7561 gtggagttac ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa
     7621 agtaagcaac actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt
     7681 tcaacacgat aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa
     7741 tcgacaagtt tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta
     7801 acagtaaacc taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt
     7861 tgttcacgat aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg
     7921 ctggcgcatt gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga
     7981 accataagaa tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc
     8041 ccataaatgg ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg
     8101 cagcaaaaat tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg
     8161 aagcaaatgc ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt
     8221 catttccgct taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa
     8281 acaaaccgtt tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa
     8341 tcacacctgc ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagaaagc
     8401 aatcaagaca gaaagaatgg caaatgtacc gttccaagca gcagccccga atcctttcca
     8461 tgattcacca ccaaaaattg agttcatcaa ttcttggaac ccgggaatcg gtaagttatt
     8521 gatcattact gctaatgctc ctaagatcat taacggcata ctcaccataa atgagtcccg
     8581 aatcgcaact aaatgccgtt gtgcaccaat ttttgatgct acaggtataa aatgtttctc
     8641 cataaatgca acaaatttat ccataaatct tccctctttt ctttactgta gattacgcgt
     8701 tcaatcgacg atacagatca atgatctctt ttgctaaatc tgtaaaagct atggatgtca
     8761 tcagatgatc ttgactgtgg actgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt
     8821 cttgtgtcag catctctgtt tgagagtgat gtgcttggac taaagattcc tctgcatctt
     8881 tgattttttc atctgctaaa gtgaaatcac cttttttcgc agcagcaatc gcttccattg
     8941 catcgctttt tgcatttccg ccaaacatga tcagacccat gatggcttct agattttttt
     9001 gatcttccat ttattgtatc ctccgataat ttttattatt tgtttattag tttttctgct
     9061 tggtcgagta ctttttcacc attcatcatg ccatagtcag ccatattgat tatatctaaa
     9121 ggaatgcctt taggtgctag cttttcagca aattgatttt gcataaagcg tacttgcggt
     9181 cctaaaagta aaacgtctac atttttagat tctagttgat tatccgcatc tgaagcagat
     9241 acagcaaaga tgtctgcttc cattcctttt gcttctgctg ccttttgcat ttttgtgact
     9301 aggagacttg tactcattcc agctgaacat acaagcatga ttgttttctt ttccatttta
     9361 aacacgttcc tttctatact tacacttgta tataagcaag tagcgtgcca actttgaaag
     9421 cgctttaaaa agttgatatc atcacattta aggtctatac caagttgcat acacactaaa
     9481 aaagaaagtg cacactaaac acacaaatta atttttatta ccttttttct tttaacgtgc
     9541 acaaaaaaag ttgtgacaca aaac
//
LOCUS       DAZKNO010000107         9517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000107 DAZKNO010000000
VERSION     DAZKNO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.109"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            588..1838
                     /locus_tag="ACG8MY_001914"
     CDS             588..1838
                     /locus_tag="ACG8MY_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0431095.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            1857..2867
                     /locus_tag="ACG8MY_001915"
     CDS             1857..2867
                     /locus_tag="ACG8MY_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFQ0431096.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            2877..3530
                     /locus_tag="ACG8MY_001916"
     CDS             2877..3530
                     /locus_tag="ACG8MY_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0431097.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3549..4547
                     /locus_tag="ACG8MY_001917"
     CDS             3549..4547
                     /locus_tag="ACG8MY_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0431098.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            4623..5384
                     /locus_tag="ACG8MY_001918"
     CDS             4623..5384
                     /locus_tag="ACG8MY_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HFQ0431099.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5402..6508
                     /locus_tag="ACG8MY_001919"
     CDS             5402..6508
                     /locus_tag="ACG8MY_001919"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0431100.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6930..7385
                     /locus_tag="ACG8MY_001920"
     CDS             6930..7385
                     /locus_tag="ACG8MY_001920"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFQ0431101.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7399..8172
                     /locus_tag="ACG8MY_001921"
     CDS             7399..8172
                     /locus_tag="ACG8MY_001921"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HFQ0431102.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8183..9289
                     /locus_tag="ACG8MY_001922"
     CDS             8183..9289
                     /locus_tag="ACG8MY_001922"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0431103.1"
                     /translation="MKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRDVPPLDPKI
                     KSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYEGGLYVLCK
                     RHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAEFPELNAKS
                     YVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMDVRKKVHFT
                     IAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAIFPYKEIYG
                     SGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKYVNLSPAEK
                     SKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
BASE COUNT         3011 a         1438 c         2228 g         2840 t
ORIGIN      
        1 acgtgaaagt agattattaa tgataatctt tatccaatta gaattattat cgtaggatgt
       61 gatgatgaaa gccgtgaaat tgttcaagta tttaccaatg tctccctgcc agtaaatccg
      121 cttagaattt gagccttgaa aatgtgcaat gataaaaaac aaaaactcgc tcaaaataga
      181 cagtttgggg tgggaataga ccaataatgt accaaacaaa cttccatttg gggtgaaaat
      241 aggtcaataa agcgatgtta aacatacctt tggagtaaga atagagggta ataagtgaat
      301 ttataaactt tttggtgact ggttgagtgg aaaatgtagg tacatttcaa tgcgaaaatg
      361 tattataacc ccaaacgagg tcaataaaaa tgactcaaac ttgcaatata accctaaacg
      421 ggtaggggat ttatgaaaaa ttgaatttgc cggcgtagga gctggctgtt ttaagtatga
      481 atgaacacag ctttcgggct gttttatata aatagcaggt tactgttcac aaacagcatt
      541 ttgttaagaa agtagtgacc acagcagtgg tggaaagagg aaatattatg aagaaacagt
      601 ataacattgc agtcgccggc accggttatg ttggtttgag tattgccact ctgttggcac
      661 agcaccataa ggttacagcg gtggatatca ttccagagaa ggttgaactg attaataacc
      721 gtaaatctcc aatccaggat gattatattg aaaagtactt ggctgagaaa gaactgaatc
      781 tgaccgcaac attggatgca aaggcagcat accagtctgc ggattttgtt gttattgcgg
      841 ctcctactaa ctacaatagc cgcactcaac attttgacac atccgctgtt gaggctgtta
      901 ttaggctggt catggaagta aatcctgatg ccattatggt tattaagtcc accattcctg
      961 ttggatacac cgcttctgtt cgtgtaaaga ctggtagcaa gaatattatt ttcagccctg
     1021 agttcctgcg tgaaagtaag gcactgtatg acaatctgta tccttcgcgt attattgtgg
     1081 gtaccgatat ggatgatgag cgtttggttg aggatgcaca cacttttgct acactgctac
     1141 aggaaggcgc aatcaaagag aatgtggaca cattgttcat gggctttact gaagcagaag
     1201 ctgttaagct gtttgccaat acatatctgg ctcttcgtgt cagctacttc aatgagctgg
     1261 acacttatgc agagatgaaa ggcttgaaca ctcagcagat cattaatgga gtttgccttg
     1321 atcctcgtat tggtactcat tacaacaacc cttcctttgg ttacggtggc tattgcctgc
     1381 cgaaggatac caagcagctt ctggccaact acgcagatgt tcctgaaaat ctgatcgagg
     1441 caatcgttga atctaaccgt actcgtaagg actttattgc ggatcgtgta cttcagatgg
     1501 ctggttatta caattacgca gaagcaaatc agtatgatgc tggcaaagaa aagcctgttg
     1561 tgattggtgt ttaccgtttg accatgaaat ccaattctga taacttccgc cagagttcta
     1621 tccagggcgt tatgaagcgt gtaaaagcta aaggtgcaac tgttgtgatt tatgagccga
     1681 ccttgaaaga tggagagact ttctttggca gtaaggttgt gaatgatttg gaacagttta
     1741 aggcaatgtg tgattctgtt atagcaaatc gctacgatgc ttgccttgat gacattaagg
     1801 aaaaagtata tacccgtgat atcttccaga gagactaaga ttaggagaga gtatttatga
     1861 ttattacaaa aacaccgttt cgtatgtctt tttttggcgg tggaacggat atggaggatt
     1921 atttcaaaga gaatggcggt gcagttcttt ctactacttt tgataagtac tgttatgtga
     1981 atgtgagaca tctgcctaga ttttttgatt acagcgctga gttatcttat tctcgtactg
     2041 agagagtaac tagcattgaa gatattgagc atcctgcgat tcgtaatgcg atgaaaatgc
     2101 tcgatatgca tgaaattcgg cttacatacg aagctgattt acctgctcgc tctggtctgg
     2161 gtacatccag ttcttttgca gttggtatgt tgaatgcttt ttatgcactg gagggtaagt
     2221 atgctgacaa gaagaaactt gctgatgaag ccatttatct tgaaagagtt ctgtgtaatg
     2281 aagccggtgg atggcaggat cagattgccg catcttatgg tgggttcaat cgaattaatt
     2341 tcaatgtgga tggttatgag gtgctgcctg tcattatttc gcctgaacgt aaaaagcagc
     2401 ttaacaacaa tctgatgatg ttctttaccg gattcactag attttcttct gatgttcaaa
     2461 aggctaacaa cgtcagtggc acagaggata agagagtaag actgaaaaag atgtatgagc
     2521 ttgtagatga agctgaggca attcttactg ataagaacag aaatttagat gatttcggta
     2581 gacagcttga tgttacttgg agattgaaaa aaggtactgg tggagcaatt agtacaggaa
     2641 gcattgatga actatatgaa aagggtatgg ctgcaggtgc tttaggcgga aagttgctcg
     2701 gtgcaggtgg tggaggtttc ttggttttct atgtacaacc tgagaaacag gatgcagttc
     2761 gttgggctat gcgtgatttg atgtatattc cgtttgaatt tgaaaatggt ggaactagag
     2821 ttattcacta cacgccggaa acttatattg aaaaacagaa tggataagga gtaacaatgt
     2881 tagcggaaaa acttaataaa catattgact tattgattaa acgatatcct acacttgaga
     2941 cgattaaaca ggacattatc gatggatacc taattatgga ggaatgctat ttgaatggtg
     3001 gtaaacttct tgtggcagga aacggaggat cagccgctga ctcggaacat attgctggcg
     3061 agttgatgaa gcggtttaag acgccacgtc ctgtaagtaa ggaatttgcc gataagttag
     3121 ttgcaattga ccctgtaaga ggtccggaat tagccaaaaa tctcgagtgt ggtttaacgg
     3181 caattccttt ggtcgcacac gaagcattgt ctactgctta tattaatgat gtcgatggtt
     3241 tgggtgtctt cgcacagcag ctgtttggct ttggtagatc tggtgacgta ttccttggta
     3301 tttcgacgtc cggaaatagc aagaatatta tgagcgcaac tgttgttgct agggctctcg
     3361 gtataaaagt tattggcttg acgggcgcaa agggtgggga acttgccacg gtagcagatg
     3421 tttgtgttaa ggctcctgaa gttgaaacat acatgattca agagtatcac ttgccgatat
     3481 atcattgttg gtgtttgatg ctggaagata aattctttgg aaacgaataa aatttggagg
     3541 aatgttaaat gaaaaagaca attatggtta ccggtggttg cggtatgatt ggctccaatc
     3601 ttgttaagag actggtgaag gaaggctgct gggatgttta tgtagctgat aatctgtgga
     3661 gaggaaagtt ggagtacctg aacgacgaag atgggcatcc tgtcatcgac ctggatactc
     3721 atttcttcaa tgctgatctg actgattatg agcaggctaa aaaggttatt ggtacgactg
     3781 agtatgtagt tcatctggct gatgttgtgg ctggcattga ctatgtgttt aagaaccagg
     3841 gcgaattgtt ccgaatcaat aatctgatca attctaatgt ttttgattgt tgccgtaagg
     3901 ctggcaagga gaaaatcaag ggtgttatct atgttggtac cgtatgctcc ttccctctga
     3961 ccagacagaa tactttgaat cctgagcctc tccgtgaaga ggagttgttc cctgctcttc
     4021 ctgagtctgc ttatggctgg agcaaactga tgggccagtt ggagatgggg tatttggaaa
     4081 aagaaaccgg aattccgtgc tgtatcctga tgctccataa tgtttatggc acacctactg
     4141 attttggcga acgtagtcag gttattcctg ctctgattcg taaagcaatc aataatcctg
     4201 aagagccttt taatgtgtgg ggtagcggag agcagggacg tgctttcatt catgtcaatg
     4261 atatcgtaaa tgcattggtg ctcgccctgg ataagggttg gggtcatggt catattcaga
     4321 ttggcccttc tcactgtact tccatcaagg aaattgcata taagatcatt gaaatttctg
     4381 gcaaagacat caagcctttc tttgatactt ctaagcctga aggtgataag gctcgttgcg
     4441 cagattattc taaggcgaaa gagattttgg attgggagcc tactgtttct ttggaagagg
     4501 gtcttcgtga atcctacaat tggattgaaa agcagattaa gggttaataa gtatataagc
     4561 tgttcttcct ccgtgtaatt agaggatgcg gaggaagaac atatgagtaa ggatgtactg
     4621 agatggaaaa gaatatgtat aaaagaaaac gtgatttcac tgtgtgtgga atagtaggtg
     4681 tttgtgctac tacaaagaac gagtttgtaa gggaagtttt ttccatgcca acatctccgg
     4741 taagtgcgac tgttgatctc gtgggagtgc cggccattat ttcggcaaag gaaaatccca
     4801 aaattgctga aatgtatagc caggctacaa tggctgcaat agatggtatg cctattgtta
     4861 aaattggacg gcgcaaagga tttacctgtg aacgatgtgc tgcaccagat attatgggaa
     4921 tggtttttga agaaggcgtt agacagggaa aaacacatta tttctatggt ggaaaagata
     4981 acgaagtgtt aaataagctt cgtgcaaacc tagaaagaga ttacccggga atcaaaattg
     5041 ttggaatgta ttccccacca tttcgtccgt taaatgaaga agaggatagc aagatttgcg
     5101 aggaaatcaa caacttaaat ccagattttc tgtgggttgg tattggtgcg cctaagcaag
     5161 aaatgtggat gtgggaacac cagaagaaga tccataacac agtaatgttc ggtgtaggcg
     5221 ccggctttaa cttttttgcg ggcactttgg aaaaagctcc tgaatggatg gaaaaagcta
     5281 gtctagagtg gttatttaga ctggtaaagg aacctcgtag actatggagg cggtatatct
     5341 tgggaggtat aaagtatatc tactatacta ttgaatctaa gtaggggggc aataaaaata
     5401 gatgaaaata cttatagtag ttacaatgac gaataacttt ggtaagaagg ggtcctataa
     5461 ttctcaggaa ataggattag ctcgagggtt agctgaatta ggccacgaag ttattgtata
     5521 taaaagtgta aaaagtgatg aggaagttgg aacagagcaa ttaagcaata cggcgatgat
     5581 tcactatatc aagtgtcgtt atgtaggatc aaatggattc ttatcaacaa agaattttga
     5641 taagaatgcg gatgttctaa tacaatttgc ggaccttcaa ctatcagtac ctcatgtata
     5701 ctcatggact gtaaaaaatg gaataaaata tataccttat aatgggataa cgcgaagtca
     5761 aagtggcaat aaatatataa agacaattat tgaaatattg gcgcatcgaa attataaaat
     5821 ttataagaaa aatgggtgtg ttgccaaaaa tgatcgagtt tctgaaaaat tagtacaatt
     5881 aggagtaaat aaagtgaaac ttgctcctgt ttgtgtggat tttgaacagt tacataagaa
     5941 ttacgaagca atagatttta ctactctcag agaaaaatgg aatattcctc gcaatgttcg
     6001 tgttatttta tatgtaggac ggtttgaaaa ggataaaaga ccgttagata tgattagttt
     6061 atttaataaa cttgatgagc ataattattt gctgatggta ggaactggtg ctttactcga
     6121 gcaaacgaag cagcagataa atttacttgg actgcagaat agagtaaaaa taattgagcg
     6181 tattcctaac aaagatattt gggagttata ttgcttatcg gatgtatttg taaatctaaa
     6241 ctatactgaa atttggggta tggttttgct agaggcaatg tactatcgcc tcgggattat
     6301 tgctgttaat gcacctggcc cacagaatat tattttggat aatagatatg gaatgttggc
     6361 taaatctgac gatgaaattt tgacattctt aaaagacgga cgatttacca acgatatcat
     6421 agatgcagca tatcagcgtg taactacaga atttagatgg tcaaagtcgg gagaaattat
     6481 gcttagcatg ttggaaggtg ataaatgata tggaagtaac gaaattaatg gcattgagga
     6541 atcgatatgc tttaaacata gttgataatt gcacgagaaa aatagccaaa attcttggtt
     6601 gttgtattgg taaaggtgca cagataggga actctgttga atttgttcat aattctgtag
     6661 gcactgtgat tcattctgac acaatccttg aagatggtgt taaagtctat cagaatgtga
     6721 cgtgtggttg agcagatagt aatgaacgta tcatcattcg agtaaaaaag aacgctgttt
     6781 tatgtgtggg tgctaaaatt ctttgtaaat ctaacacaga attatgcgtt ggggaaggtg
     6841 ctgtagttgc tgcaaataca gtggtattga ggtctattcc accctacgaa gtgtgggggg
     6901 tacctgctaa aaagatgggg agataaaaca tgaatgcata taaattatac aaagtagaac
     6961 gatggtttta cttacacaaa atggagtttt ttgccaagat tgtaaaggca ctaatttatg
     7021 tacttcataa tagctcaatt tcatatcaaa cagaaattgg agataattgt aagttcttat
     7081 acggtggcat aggttgtgta attggaaaag atacaaaaat aggtaatcac gttataatcg
     7141 gaacgaatgt actgactggt ggtagatcca ataagcctgg tatgcctaca attggaaaca
     7201 atgtatatat cgctactggc gcaaaaattt taggtaatat cacaataggg gacaatgtaa
     7261 tagtaggggc taatgctgtg gtcattaatg atgttgaacc taattgttcg gtaggaggcg
     7321 ttccggctag agtattgaaa aaagatattg atgtaaatga ttattgtagt ttgaatcgtt
     7381 actaaaagga gagattgtat gttgtatttt gttattcctt taatgagtgc gaaggcagca
     7441 aacaattggg agatggttag taccttattt aaccgaacat tatggtcgtg ttataatcag
     7501 actgatcctg atttcaaaat cattgttgct tgccatgaaa ttcctaaact cacgcatgaa
     7561 tatgatgaca gagttgaatt tattcaagtt agcgaaaagg atgcccctat tcccacaaat
     7621 caagacgaaa aaatgattga taagggttat aaaacacatg ctctcgcaat gcgtttgcga
     7681 gagttgggcg gcggtttcgc tatgatggtt gacgcagatg atcttgtaag ttctcatttg
     7741 gcggaatatg tgaattccca tccccaggaa aatggatttt atgtcaaaac gggatacgtg
     7801 tactttgttg gagacgatta tatgaaggtt ctaccaaagt tctcatcagg aagtgcgtgt
     7861 atagtaaaat atgcagtgga agacctgcct gaggcatatc cggaagttat gacagcaaat
     7921 tgtgatgata atccatggat cattagaaaa cgacatgggg gcgttgttcc agcatgtgaa
     7981 gagcaagggc gtccattaaa acctcttcca ttcaaagcag cagtatatgt attaggaaca
     8041 ggggacaatc actcgcttta tggtaaaacg acaaaatatc agacgaaaat gcgagagata
     8101 ctggaaatgt ttatagcaaa acgtaaaatt aaaggaaaac tgaaagcaga attttctgtt
     8161 gattggttat aaggtgagta ttatgaaggt attgatgtgt gaaagccaaa tgccagagaa
     8221 ttggtattcg tttgatcttt ctaaagaact taatcaatat gttgatttaa cattgctgtg
     8281 taagcgtgat gttcctcctt tagatcctaa aattaagagc aaacatattt tgtatcataa
     8341 aagtacaaat aaagtgtttt cggcacttgt ttttctgaaa agcttgttct taatttggaa
     8401 ggaaataaga tttggtgatt atgatattgt tcatttgcaa ttgatgaaaa gtctcaaata
     8461 tgaaggcgga ttgtacgttt tatgtaagag acactataaa aaattaatta tgacagttca
     8521 taatgtgtta ccacatgagg ttcgtgaaaa ggatcgccaa gagcacttta atatatattc
     8581 aaattgtgat ggtttcattg ttcacaatcg agtaacagaa cagcatttat atgctgaatt
     8641 cccagaatta aatgcaaagt catatgtttg tccacatgct atctatggag atgttaaaca
     8701 tatatctaga gcgtatgata aagataagtt tgaatttatc ttgtttggac aaattcgtcc
     8761 atataaggga attgatgtac tgcttaatgc aatcacagaa cttccaatgg atgtaagaaa
     8821 gaaagttcat tttaccatag caggtcaaca acatcctaaa caggatagta ctgactataa
     8881 aaaaatgatt gaagatttaa aggtttcaga ttgtgttcta tataagccag agagaatttc
     8941 tgatgaagaa atggacagta tgttcagaaa tgcagatgcc gcaatatttc catataaaga
     9001 aatctatgga agtggagcgc ttcttatggc atacacttat tgcttaccaa ctattgttag
     9061 taatgtacct gcttttatgg aagaaaccga ggaaggaaag atagggttac ttttcgaaaa
     9121 tttgaattct aagagcctag cagacacaat tgtaaagtat gtgaacctta gcccagctga
     9181 gaaaagcaaa atagaggatg atatatatga gcttgtaaac cgcaagtata cctgggcggc
     9241 atctgcaaaa ctcacatacg aggcatacaa acaaattttg aatgagtagt aaaacaatta
     9301 gaaaggaata ataattaaca tgcagattag taaatggaaa atcatacagt ttctagtggt
     9361 ctttctaatg attatctcat caagcatatt gaagcctttg ggcggctggt tgggagaaac
     9421 gatgaacgtt agacttgtga cagtcattct agcaatagta tgtttgttaa tagcctcatt
     9481 accagcattt agaaaaaata gatatttaaa tttctgc
//
LOCUS       DAZKNO010000108         9277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000108 DAZKNO010000000
VERSION     DAZKNO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.110"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1447)
                     /locus_tag="ACG8MY_001923"
     CDS             complement(<1..1447)
                     /locus_tag="ACG8MY_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431104.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSV"
     gene            complement(1545..1820)
                     /locus_tag="ACG8MY_001924"
     CDS             complement(1545..1820)
                     /locus_tag="ACG8MY_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431105.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            2052..2786
                     /locus_tag="ACG8MY_001925"
     CDS             2052..2786
                     /locus_tag="ACG8MY_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0431106.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            2825..3967
                     /locus_tag="ACG8MY_001926"
     CDS             2825..3967
                     /locus_tag="ACG8MY_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0431107.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(4062..5453)
                     /gene="sufB"
                     /locus_tag="ACG8MY_001927"
     CDS             complement(4062..5453)
                     /gene="sufB"
                     /locus_tag="ACG8MY_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0431108.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5458..5928)
                     /gene="sufU"
                     /locus_tag="ACG8MY_001928"
     CDS             complement(5458..5928)
                     /gene="sufU"
                     /locus_tag="ACG8MY_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0431109.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(5915..7150)
                     /locus_tag="ACG8MY_001929"
     CDS             complement(5915..7150)
                     /locus_tag="ACG8MY_001929"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0431110.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(7147..8433)
                     /gene="sufD"
                     /locus_tag="ACG8MY_001930"
     CDS             complement(7147..8433)
                     /gene="sufD"
                     /locus_tag="ACG8MY_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0431111.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(8446..9219)
                     /gene="sufC"
                     /locus_tag="ACG8MY_001931"
     CDS             complement(8446..9219)
                     /gene="sufC"
                     /locus_tag="ACG8MY_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0431112.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
BASE COUNT         2717 a         1887 c         1647 g         3026 t
ORIGIN      
        1 tcacagaagt tgcattaaaa acgtctaatc gatagacatt gcttctctta tgattaaagt
       61 ttcctaacgc tttctcttcc tttaacctat ttaggtattc gagcatcttc aaaggaacat
      121 cttcatcatc taattttttt atcaatccta taaaagtcat aagtgatagt aacttattac
      181 atttatctat gtcataagtt ttaccatagt aagaactcat ttttttagct aattgttccc
      241 ccgacaaaat acaagtcaca ctttccttac tatcatcaaa aaaattaaca tcagaaagca
      301 agacatcata gacacgtcgc atttcctcta gataattcct tattgtcctg aaaagtttgt
      361 ctttcattga ttcaagagtg tcaaaaagta cttttgattc ctctttcaat tttcgaaaat
      421 gtgtaggata caagtctagg ttctctaata gccaattaaa tgtttctacc cgactgtctt
      481 tctttaaaat tagcatcaca actccaataa aattttcact gatatcacaa ttactggaat
      541 gacaataaaa aatggaatgc ccagttttac tggtaaaaat actagcagag ggattatgat
      601 cgttgtgaaa cagacatctt aagtttttac ttgacttaag taatttattc atatcaatgg
      661 ttaacaatct ttcataaatc tcattaaaag acatgtcgga ggaatcaaac aggcagtctt
      721 taaaccactg tttcatttct tctttgtcgt cattttttat caacttaaca aaatcggttt
      781 cattgactag ttcatactct ctccctgaat cactttgaca atttttagaa catgtcatgg
      841 taaaatcaat cggaagctca gtaatatcta acatgttgtc ttcattaatt attatcactg
      901 catctgactt atatcctcca aaaaacattc tattcgaagc atttacattg tcatcacacc
      961 cggggatgtt ctttttcaag tattcgtaaa cagcaaccat tttaccatag tcagtaattg
     1021 cttcttttaa tagaaagaca atcttgtatt tttctagatt ttcagagtac cgaatacttt
     1081 tttgtatcgc aaacgcagaa ttatgaacta aattgataga taaaaccttt tctatgctta
     1141 agtatctact atcatcttta ctcaaatcta ctaccttccc cttctcatct ttaacagtgt
     1201 tatcgacatc cagaataatt actctggttt ccacaaaact ctccttgtca ttgcctttat
     1261 tctttgtctt gcttggaata aatgattgtg atgaatttag tatctctaca aactcatctc
     1321 ttgtaacttc tctatagtta tctaaacacc tatctcttac atcagttttt tctttttttg
     1381 taactggctt ctctaataat ttttcttgat ctacataaat tggaatatta atcattgatg
     1441 tttccatttg tgtttcctcc ttataaaatt ctgttaaaga ctaaaacata cagataataa
     1501 aaacaaactc tttattacta tatgttttag tcttatttgt ttactcaacc tttataccgt
     1561 tccatgaaac tatcaatatc agatttttta aatctaatga ccgtatcaat ttttataaca
     1621 ggtaacccgt attgagaaac atattttcta atcgttccta ctgatacacc tagatattca
     1681 cttgcctgct gatagtccat atactctaga gtgactccag aaataatgtc ttctagcata
     1741 ggctttagca ctgtaattat ctctttcact tgttgttcaa cttgtataga aacaatgcta
     1801 tcaatgattt gtagagacat atttccccct cctttcgtga ttgcttgaac aaattttcta
     1861 agaattatct tcgtattttt tgctcaaaat aaaagtattt tgttattaac cgattaatca
     1921 aacaaaaaat gattaccagt tcatcgacat acacataata cctcgataaa aaaggggtgt
     1981 caacagcttt gtaaaattta tttacaacac cccttttttg aggtatacta attttaggag
     2041 gtgtggtcaa tatgataaaa cgtccaccaa taaattattt agaacgaaaa aagatactcg
     2101 gaacaaaaat aaaagctata agaaagagta aaaaattaac acaaccagca tttggtctta
     2161 tgattaacaa cggtcaatta attgacaaaa aaactatcta cgaatgggag aagggaacct
     2221 atttaccaat accagagcga ttatcccgaa ttgctgattt gggaaatatg agtatagagg
     2281 agttagtttg tggcaatgta gaagaatata ttttgggaat tattctttat agagatagca
     2341 ttgtactaga tggtataaca tttccagata aaaacctgtt ccagcatctt cgtcaacagt
     2401 tcccaccagt acactctaat ttagatacat ggctggatag atattccaaa ttagaaccag
     2461 agatgcaaga atttattgct aacaagactt gtaataaagt taaaaatgaa aaaattagtc
     2521 ttttcaatat cttaaaaata gaagaattat tcatcaatgc tattgtagaa gaatttgaca
     2581 ataacattct tttcctcact agcagtatag aagaactact agaaagaatg gtagatgaat
     2641 ggctacctat tcaattgaaa gacatgtctt atcccgaaga agctgtgaga gaaattactg
     2701 ataatataaa caagctagaa caaacgattt catcaattgg gaaaaaatac actaagaaaa
     2761 aaatgaaggg aggtgatacc atctagtggt aagtactcct tgtaaactaa gaggagatga
     2821 tttaatggca aaaatatcaa agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa
     2881 aggacacatt gccaccgatc cacgaactgg tgaaaaaata aatactacga gaagaggttt
     2941 tagtagcaaa caagaagctc aaaaagatta tgacgagtac agacatcaaa tattatacgg
     3001 tgttaaaaag aaagcacctg atatgacatt tgaagacctg tataatgaat ggataactca
     3061 tcagcgtacc agtgttaaag cttcaacaat cgcaatctca gtaagatacg caaataatca
     3121 aatacttcca gcatttggta agcttaaact atcgaatatc tctgtcccat attgtcaaaa
     3181 agtggtagat gaatggcaca gtaaatatga aagttacgat tatatgagaa aacagactgc
     3241 acaaatttta cgctacgggg ttgcaatgca atatattgac aataatccta tggaaaaaac
     3301 acttcttcca cgaaaaaaag agtatgagaa aaatcgtaaa ttttactcaa aagaggagct
     3361 taataattta ctggatgctt ttaaagattt tggtaacatg aaacaatacg ccttcttcag
     3421 attattggca tacacaggta tgagaaagag tgaagtactc gctttacagt ggaaagacat
     3481 agacacgttt aataaagaac tacatgtcaa taaaactctt gctgtagatg aatttggtaa
     3541 agttataatt caatctccga aaacaagagc ttctagacga gttatctctt tagatactga
     3601 gactctttct atcctcaaca actggaaatt acagcaaaaa gaagagtatt taaaacttgg
     3661 atacaatact agctctaaag agcaacatgt ttttacaaca gttaaaaaca ctctatacat
     3721 tccaaacact gttaatgatt ggttaagata tattttgaaa aaatataact tgcctcgtat
     3781 tacacctcat gggtttagac atacacatgc tagtttatta ttagaagcag gagaatctgt
     3841 caaggtagtt caacaacgat tagggcatga aaatagtaaa gtgacattag acatctatgc
     3901 acatattaca aataatgcac ctaagaaaac aggtcaagac tttgctgata tgatggctca
     3961 tcagtaaaag agctggtcaa aatctggtca tttctagtat acaggcaaaa caaaaaagct
     4021 agaaccctta tatatcaagg gttctagctc ctataacagg tttacccaac actgccttcc
     4081 atctcataac tgatcaatcg attgagttcg actgcatatt ccattggaag ttctttggtg
     4141 aatggttcaa caaagcccat gacgatcatt tcagttgctt cagattcggt caggcctcgg
     4201 ctcatcagat agtagagttg ttcttctgag atttttgaga ctttggcttc gtgttctaaa
     4261 gagacttggc tgttgtggat ctcattgaat gggatcgtat cggatttcga ccattcgtcc
     4321 atgatgatcg tgtcacattc gatatgagag atcgaacccg cactgttttt ggcaaaggtt
     4381 acttgtcctc gatagtttac ttctccgccg tcttttgaaa ttgatttaga gacgatagag
     4441 ctagatgtat ttggtgcatt gtggatcatt tttgcacccg tatcttggat ttggtttgct
     4501 cctgcaaagg cgatggagag catggtgcct cgtgcacctt ttccatctaa gtaaacactt
     4561 gggtatttca tcgttgtttt tgcacctagg tttccgtcga tccattcaac ggttgcgcct
     4621 tcgtatgctt ttgctcgttt ggttactaag ttgtacacat tgtctgacca gttttggatc
     4681 gttgtgtaac ggcaatacgc atcttttctt gtaaagattt ctacgatggc ggcatgcagg
     4741 ctgttacttg aataagtagg tgccgtacaa ccttcgacat agtggatgct tgctccttcg
     4801 tcaacaatga tcagcgtacg ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac
     4861 gtttgtaatg gaacatcgac tttgacgcct tttggcacgt aaataaaggt cccacctgac
     4921 catacggctg aattcagcgc tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag
     4981 tactctttga ataattcagg gtattctttc aaagcagagt cagtgtcagt aaagacgatt
     5041 cctaactttt cgaattcatc tttcatatta tggtacacga cttctgattc atattgtgcg
     5101 gaagcaccgg ctagataagc tcgttcagct tctggtatac caatcttttc aaatgtatct
     5161 ttgatttttt ctggtacgtc ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa
     5221 tatttgatgg catcgaaatc gatgtctgaa aggtcggggc cccatgtttg cattggcatt
     5281 ttgttaaatg tttctagaga tttcagacgg aattcaagca tccattctgg ttcgcctttg
     5341 attcttgaca tctcacggat cacttcttct gtcaagcctt ctcctgtact gaaaaccggt
     5401 tcgacatcat cgtgaaaccc gaatttatat tcttctaatt ctggtacgcc catgttctca
     5461 ctcctcttcc ttgtggtaat gatggaaagc tgcggtacca tcgttttcca ttgcttttcc
     5521 tagtgctttc caggcaagtg tcgcacattt gatccgagcg ggaaatttag ctacaccacc
     5581 aagcatagca gcatcgccta attggtcggg atctttgact tcttttcctt ggacaagctg
     5641 tagaaagtct acagttaaat gttctgcttc ttctattgat tttccgatga cggcgtctgt
     5701 catcatcgag gcacttgccg tactgatgga gcagccactg ccgtcaaagg cgatattttt
     5761 gatgattccg tcttttacat cgacttgcaa ttcgatcaca tctccacatg tggggttgtt
     5821 caattcgatc ttgcttgtcg agtcagtcaa gcttccacga tgatgcggat gggaagaatg
     5881 atccaagatc acttgacggt ataaattgtc taatttagat agtcccatgt tggaaaaact
     5941 cctttgttgc catgattgct tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat
     6001 aaaagctcgc gcgagcagtc gctgatactt caaggtactt caatagcggt tgcgcacaat
     6061 gatgacctgc tctgacggct accccttcca tgtctaaagc tgtcgcgaca tcatggggat
     6121 gtaacccatc caaattgaat gcaacgactc ctgtccgaaa attcgggtct tgtggaccat
     6181 aaacagtcaa tccatcaatt gcttgtaatt ttggcaacac ataagcggct agttcttctt
     6241 catatcgatg gatattgtcc ataccgagtt tttgcaaata atcgattgca tggcctaatg
     6301 cgatagcgcc ggcgatattt ggtgtccctg cttcgaattt ccaaggtagt tctttccatg
     6361 tactgtcgta taattcgaca aaatcgatca tttcgccacc aaattcaaca ggttccattt
     6421 cttctaacca ttttcgttta ccatataaaa caccaatacc tgttggtccg cacattttgt
     6481 gtccgctgaa agcaaagaaa tcacagtcta gtgcttggac atcaatggcc atgtgcgggg
     6541 cagattgagc accatctact acgatgaccc cgccgttttc atggacaagt tcagctaatt
     6601 cttgaacagg attgaagaca cctaagacat tggatacatg agcaatggag acgattttgg
     6661 ttttgtccgt aatctttttc gctgcatcct ccatatccag gaagccttct tctgttagtt
     6721 cgatatattt caagacagca cctgttcgtt ttgccaactg ctgccacggg ataacattag
     6781 aatgatgttc catgtaagag agcactatct cgtctcctgc tttgatatag cggtcgccga
     6841 aggttctggc tacccaattc aagcttgtcg tcgtaccacg agtaaacagc acttctgctg
     6901 tttcttttgc atggatgaac gcccgtactt tttctcttgc ttgttcataa tcatgggtcg
     6961 cgcgttctgc taacgtatgg acaccgcgat gaacattggc gttatcgtgt tcgtaatagt
     7021 acgctaatgc ctggagtact tgttttggct tttgggtcgt cgctgcatta tccagataaa
     7081 ctaaaggttc atcattgacg tcttgaaata agataggaaa atcattgcgt agctttttag
     7141 catcgatcat ccattcaact tcccttcgat tacttcgact aattctttcc gaacttgttc
     7201 tacagggatt gctgttaaga cagaaccgag gaatcctcga atcactaagc gctctgcttc
     7261 ttctttatgc aatcctctac tcatcagata gtacatttct tctggatcaa ctcgtcctac
     7321 actggcagca tgtccagcag ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc
     7381 accacgtgct ttatcagaaa gcatcaagac acgactttct tgttgggcat ctgcaccttt
     7441 tgcccctttc aagatgtggc cgataccatt aaaggtcaat gttccttttt ctcggatcac
     7501 tccgtgttgc aaaatatgcc cgatcgtatg aggggcttta ttggttactc gtgtatcgat
     7561 tccttggatt tgtctgcccg agctgattgc cacgattttg acttctgcgt gtgcaccttc
     7621 tcctgctaaa tctgagtcaa agtcagcgat cacatgaccg tcattcatga caccaattgc
     7681 ccagtcgaca gaagcatctc gtaaaatgtg cccacggcga ttcatataag aagtaatatt
     7741 ttctcctaat tgatctacgg ctgaatattt gatttttgaa ccagctttag cgatgacttc
     7801 cacgatgata ttacctgatg attttgctac ttgttctttg gttgtctgga aacgttccag
     7861 ataagaaaat tcactatgtt catctgccac gatcaacaca tgcttgaaga agtgctcatc
     7921 ggatgcgcca tcttgaatga ataaggactc gagtggttca tcgatcacga catttttagg
     7981 cacatacagg aaaataccgc tgttcataaa agctacatgg agtgcagtca attggtcttc
     8041 gttcatgttt actgcttttt tcatataata ttcttggact agttctggat attcgatcat
     8101 tgcagtgaac aagtcggtaa aaatgactcc tttttctgct aattccacag gcagttgttc
     8161 aaaaatcgtc catgaacctt gttggacaag cacaggattg tctttcattg catcaaatgc
     8221 cggaatcgtg cctgtttcac tgatggtttg tgtttctttc acatcaaata acgaccagcg
     8281 atggaatttc acccgatcga tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc
     8341 ttgacggaag gttctcatcc aatcaggttc gcctttactt aaagaaaaat catttaccgc
     8401 gtcaagataa tcgagccagt ttttttcttt cattcttgct cccccttacg cttcttcttc
     8461 tttatagtcg attcctaatt ctttactgat accagcgtag ccttctgctt ccagacgttt
     8521 agctaggtca gcattccctg tcatgactac tcggccttcc atcatgatgt gaacgacatc
     8581 aggtgtgata tagttcaata atcgttgata gtgggtgatg atcaatgcac cgaaattgtc
     8641 tccgcgcatt tcatttactc ctttagcaac gactttcaaa gcatcgatat ccagacctga
     8701 atcgatttcg tctaaaattg caaatgtagg ttctaacatc aacaattgca ggatctcatt
     8761 ccgtttcttt tctccgccag aaaaaccttc gttcagataa cgttcagcca tttcttctgg
     8821 catatttagt aaagccattt tttcatctaa ctttttcaaa aatgccataa cagaaatttt
     8881 gtcgttttca tcacgtttcg cattgatcgc tgcacgcatg aattccgcat tcgtgatacc
     8941 tggtatctca cttggatatt gcatagctaa aaataagcca agtcgcgcac gttcatctac
     9001 ttccaagtct aaaacatttt tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt
     9061 tggattgccc atgatagcgg cagataaggt agattttcct gtgccgttag gtcccatgat
     9121 ggcatggatt tctccggttt tcattgttag gtttacgcct ttaaggattt ctttttcctc
     9181 gatcgacaca tgtagatttt ttatttctaa gacagacatg cattataccc ccaaattctc
     9241 tttcgttctc actcatttta gactaattga gaatgaa
//
LOCUS       DAZKNO010000109         9256 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000109 DAZKNO010000000
VERSION     DAZKNO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.111"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..1103
                     /locus_tag="ACG8MY_001932"
     CDS             129..1103
                     /locus_tag="ACG8MY_001932"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0431113.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            1273..2673
                     /locus_tag="ACG8MY_001933"
     CDS             1273..2673
                     /locus_tag="ACG8MY_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0431114.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(2751..3473)
                     /locus_tag="ACG8MY_001934"
     CDS             complement(2751..3473)
                     /locus_tag="ACG8MY_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0431115.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3552..4058)
                     /locus_tag="ACG8MY_001935"
     CDS             complement(3552..4058)
                     /locus_tag="ACG8MY_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0431116.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            4181..5980
                     /locus_tag="ACG8MY_001936"
     CDS             4181..5980
                     /locus_tag="ACG8MY_001936"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0431117.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(6048..7622)
                     /locus_tag="ACG8MY_001937"
     CDS             complement(6048..7622)
                     /locus_tag="ACG8MY_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFQ0431118.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(7877..8386)
                     /gene="mreD"
                     /locus_tag="ACG8MY_001938"
     CDS             complement(7877..8386)
                     /gene="mreD"
                     /locus_tag="ACG8MY_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0431119.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(8390..9244)
                     /gene="mreC"
                     /locus_tag="ACG8MY_001939"
     CDS             complement(8390..9244)
                     /gene="mreC"
                     /locus_tag="ACG8MY_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0431120.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
BASE COUNT         2802 a         1892 c         1755 g         2807 t
ORIGIN      
        1 tgtaagcttt tacatataat acaaccttgt tatctgttat attttatagc caaatagcgt
       61 gacatggggc ttgagaaatg ttagcataag catgggaaaa aacaaatcaa tggaaggatt
      121 gatactaaat gactgcagct gcagaaaaaa aagatcatca aaaagttatt cttgttggag
      181 acggcgccgt aggttctagc tatgcctttg ctctagttac tcaaaatatc gctcaagaag
      241 taggtattgt ggatatcaat actgctaaaa ctgaaggaga cgcaattgac ttgtctcacg
      301 ctttggcatt tacttcacct aaaaaaatct attctgctac ttacgcagac gcacatgatg
      361 ctgacttagt tgttattaca gctggcgcac cacaaaaacc aggtgaaact cgtttagact
      421 tagtaaacaa aaacctaaaa atcaaccgcg atgttgtcac acaaatcgtt gaatcaggat
      481 ttaacggtat ctttttagtt gccgctaacc cagtagatat tttgacttac tcaacttgga
      541 aattctcagg tttccctaaa gaacgcgtaa tcggttcagg tacctctctt gactcagctc
      601 gtttccgtca agcacttgcg gaattagttg acgttgatgc acgtaacgtc catgcttaca
      661 tcttaggtga acacggagat tcagaattcc cagtttggtc acatgcaaat gttgccggct
      721 tgcaaatcta tgaatgggta aaaaacaacc cagaaatcga tgaagaagct atggttaacc
      781 tattctttgg cgtacgtgac gctgcttaca caattatcga gaaaaaaggc gctactttct
      841 atggtatcgc agtagcatta gcacgtatca ctcgtgccat cttagatgat gaaaatgctg
      901 ttcttccttt atcagtattc atgaatggcg aatatggatt gaacgacatc tacattggtg
      961 cacctgctgt tatcaaccgt caaggtatcc aaaaagtaat cgaaattcct ttaagtgaca
     1021 gcgagcaaga ccgtatggct gcttctgcta agcaattaaa agaaattcta gatgaagctt
     1081 ttgctaaact agacgctgaa taatctttga acatgacgga aaacatctat tcggtgtttt
     1141 ccgttttttt attttatcac ccttatggtt tctaaatttt ttcataaagt agagataacc
     1201 ttcgtctttt cctctaaatt atgctatcat gagaccgttc caccctaaac ttttacttaa
     1261 ggagttgtcg atatgacaag atcttcgcat cgaaaaaaat cgaaacatgt tgctttgctt
     1321 tcagttcttg gtgttctctt agtgctgatc agcttttata cctatcgcag tacatactac
     1381 acaaagcatt ttttgccgaa tacagaaata aatggaataa atgtcagcaa tctaacagtc
     1441 gaaaaagcaa atgaaaaatt aaaagaagct tattctgata aaaaactggc tattaaagaa
     1501 gatggaaaag tctggcagga agtaccaaaa tccgagctcg gctacaaaga tgatttcact
     1561 tcggagcttt ctccgatcct aaatgaacaa aacggatgga cttggggaat gacttatgtc
     1621 tcagcggcag aaaaacaaga aatagatcct gtcagtcaaa accaccaaaa actagacact
     1681 accgttcaga cattgacaac taagctgact gatttgaaca aagaccggac acctactact
     1741 gatgcaacga tcgaaaaagc aggtgactct ttcgttatca agcctgaagt caatggaaat
     1801 acgatcgacg tcgatgctgc ggtcaaacaa ttaaaaagtg cggtaaactc tggtaaagat
     1861 acaattgaat tgactgaatt caaagaaaaa ccaaaagtaa catctgaaga cagctcactg
     1921 aaagaacaac tcgcatccat gaatgctatc gcaaatgtaa aagctactta cagtataaac
     1981 ggtgagactt tccagatccc atcaagtgat attatgagtt ggctgaccta caatgatgga
     2041 aaagtcgatt tagatacaga acaagtacgt caatatgtaa cagatttagg cacaaagtat
     2101 aacacaagta cgaatgacac aaaatttaaa agtacaaaaa gaggagaagt tacggtccca
     2161 gttggcactt acagttggac gatccaaaca gacagtgaga cagaagcatt gaaaaaagcg
     2221 attctggcag gccaagattt tactcgttca cccattgtgc aaggtggcac aaccgctgat
     2281 catccattga tcgaagatac gtatatcgaa gttgatctgg agaaccagca tatgtggtat
     2341 tacaaagacg gaaaagtggc ccttgaaaca gatatcgttt ccggaaagcc aactacacct
     2401 acacctgctg gagtcttcta tgtttggaat aaagaagaag atgcaacttt aaaaggaaca
     2461 aatgacgatg gaacacctta cgagagtccg gtaaactact ggatgccgat tgactggaca
     2521 ggtgttggta tccacgattc agactggcaa cctgaatacg gtggagactt atggaaaacc
     2581 cgcggttcac acggctgcat caacacccca ccaagtgtaa tgaaggaatt attcgggatg
     2641 gtcgaaaaag gaacgcctgt attggtcttc taacaaaaat aatatataaa aagaaagcac
     2701 ccgtacgatt ggtacaggtg ctttcttttt attatctaac aatgtgctac ttacatttca
     2761 gcagcagtaa agacttctga aacatcatca tcgtcttcta gcttatctac tagacgttcc
     2821 aattgctctt tttgttcctc atttaaatca atcgtcgttt gtggaatcat cgtcaattct
     2881 gcttgcgcca aagtgaactc tttttccaac tcatctcgta cagcagcaaa atcttctggt
     2941 gcagtataaa tctcgaatac ttcaggagaa gtttgcagat cttctgcccc cgcctctaat
     3001 acatcctcaa acatcgtctc ttcatcaaca gtcaacccat cgcgttcaat agcgatatat
     3061 cctttgcggt caaacaagta actgactgaa cccgtttctc ccagtgatcc accgtttcga
     3121 gtaaaagcta ctcggacatt tgtcgctgtc cgattacggt tatctgtcaa tgcatggact
     3181 aagattccta ctccacccgg accatatcct tcatacgtga tttcatcgta attcgtttca
     3241 tctacggcac tcgtagcctt tttaatcgcc cgttccacat tgtcgttagg catatttgca
     3301 gcttttgctt tgtccacaac aagccgcaaa gctggattca tagatggatc aggaccgcct
     3361 gctttagctg ccatataaat ttcacgagaa agtttctgaa agactttccc tcgttttgca
     3421 tcttgggcat tttttcttcc ttggatatta tgccatttac tatgtcctga catgattttt
     3481 cctcccttgt tcttttgaga atacacttct attctagtac tatttcacaa aaaaacaaag
     3541 ccacagccaa tctataaacg cttcttgaat gccgcaaaaa gcacacataa tatcacagca
     3601 gtcagagcac cgatcgaatc caacatgaca tcttggaaca taggtgttcg tccaccggtc
     3661 atcatctgat ggaattcgtc caaacctgcg taccctgttg ctgaaagcca agcaaggatc
     3721 gctgtcagcc accaaatttt tagcttgggg aaaacaccta aaaacaagct gcctcccaaa
     3781 ataaaatagg taccgaaatg tgcgcccttc cttaaaaaga actctaaaaa tttggcgtag
     3841 cccagatggt cgatactcac catacttcca ccataattaa aacgtatacc cgctaatgtg
     3901 tctttaaatg gctgattagg cagccagctt tctaatcgcg aaacttgtga ttgctgtccg
     3961 taagtttgag aagaactgta aaataagatt ataacgacta tcagtgcgat tactaaataa
     4021 atatttccgt ttttcatttg tttttttgaa gaattcataa gtcacctctg tattttatct
     4081 tagacttttt ttgaagaaac aacaatcatt ttcatagaga cttcttccat aaaaaagtgc
     4141 tatacttaat tttgtattac aatatgaagg gagttttttc atgagttatt cattaaattg
     4201 gaatctagat tctatttttt ccggaggcag tcattctgat gctttgaacc aacggatgaa
     4261 gcaattggaa gaccaaatga acgagtatta tcaccgcgta acaaaatggt cacctagttc
     4321 tgacaatgca gagcagttaa acgctattct tcaattgcaa gaaacgatta cgaatggttt
     4381 tacccaatgc agcagctata ttactgcatt gctttctgcc aatgtcaatg attcagatgc
     4441 aaaggtctta tcaggcaagc tttacgcaat gctgcctcgt ctgcaatcag ccgaaactgt
     4501 attgtcaaag aaatttgcag aaatatctga caatgattgg aatcagatgc tttctcaatc
     4561 ttcgtttgaa acaattgctt ttcgtttaaa cgagattcgt cgtgatggct cacaattatt
     4621 atcagaagct gaagaaaaca ttataaatac gctttcttta gatggtctaa atgcttggag
     4681 cagtcattat gatacgattg tagccagcat ttcgattccc ttcgaacaag atggacaagt
     4741 agtcgaatta tctgctggac aagccttcaa taagatgatg ggcgatccag atccaaaagt
     4801 cagagaaaca cttttcgcag catgggaaga agcctggaaa gaaaaggcac ccctcttcac
     4861 ggatactttg aatcatttag atggttttag actttctgac tatgaacttc atggtgtcac
     4921 agattttcta caaaaaccat tagagtacaa tcggttgaag aaagaaacat tgtctgtcat
     4981 gtgggataca atccagaaaa acaaacaacc ccttgttaat tacttgacta gaaaagctaa
     5041 tcttttcggt aaagaaaaaa tggaatggca agatcaagat gcacctatta ttttaggcga
     5101 tttgaaagaa aaaaccttta gttttgacga agcagcagca tttattatcg aaaactttaa
     5161 caaattcagt ccaaaaatgg ctaaatttgc ccaatctgct tttgaaaaga gctggatcga
     5221 agcagaagat cgtcctggga aacgtcctgg tggttattgt actgagttac cagaaacaaa
     5281 agaatcaagg atctttatga cctacagtaa ttctgttaat gaggttgcga cacttgctca
     5341 tgaactagga catgctttcc atagcagcac gatgtgggat ttaccttctt taaatcgcga
     5401 atatgccatg aatgtcgcag aaacagcaag tacattcgca gaactgatcg tagccgatgc
     5461 tacattaaaa gtcgcaaaaa caaaagaaga aaaaatcaat ttgctagata caaaactgca
     5521 aaatgcttta gctatgttca tgaatatcca ttcccgattc atttttgaaa atcgattcta
     5581 tgaagcacgg caagaaggat tagtctcaga agataagatc acagagatga tggttgaggc
     5641 gcaaaaagaa ggctatcaag gagctttagc tacttaccat ccatatttct gggctgcaaa
     5701 attgcatttc tttattgatg acgtaccgtt ttataacttc ccatacacat ttggctacct
     5761 ctttagtttg ggcatctatg cttacgccaa ccaaaaggga gcaaattttg aagaagaata
     5821 tatccattta ctacgcgata ctgcctcaat gacgacggaa gatcttgcga agaaacattt
     5881 gaatgtcgat cttacccaac cagatttttg gcaagcaggt atcgacatgg tgactgaaga
     5941 tatccaaacg ttcatggaat tgacagaaga atttatctga tttctatttt atcacaataa
     6001 aaaaagagtt agtccagaat gggctaactc tttttgatta atcattctta catgctgaca
     6061 gcaaagtcag gggcaaacca ttgaactgaa ccaactttta cgctttcgcc tggttgagga
     6121 gcatggatat attgtcctcc gcctaaagca atcgctacgt ggtaagttcc gccttgtgac
     6181 ccccagaata acaagtctcc agcttttgct tgagaaactg aaatttttgt tccagctgat
     6241 tcttgaggaa ctgtccaacc accgatgtca cgaccagtta cttgcatgta aacatagcgt
     6301 gtgaaccctg agcagtcaaa gccacttgga tctttaccgc cccaaacata tggagtacca
     6361 atatatttgt aagcttctgc tacgattgct gcaccattca cacttccaga aggagctgga
     6421 gcaggtgtta caggttttgg ttgttcaggt gttggtgttg gcgttggagc cggagtacta
     6481 cttcctgtgc cgtttcctgt atcaacactt tgatctgttg atggtgttgt aggcgcaggt
     6541 gttgttgatt cttctgttgt tgaagattca ggcactgttg ttgattcttc tgttgctgaa
     6601 gattcaggca ctgttgttga ttcttctgtt gctgaagatt caggcgctgt tgttgattct
     6661 tctgttgctg aagattcagg cgctgttgat tcttctgttg aagattcagg tgttgttgat
     6721 tcttctgttg ctgaagattg tgtagctgat gatgattctt cagtcattga agattgtgta
     6781 gctgatgatt cttctgttgc tgagctttgt gcagtagatg aagcttgagt agcttgtgct
     6841 tcagcctctg cttgttcgcg tgcttctttt tcagcttttt cttgtgcagc ttgttgacga
     6901 gcttgttctg ctagacgttg ttgttcacga atacgagctt gctctgcttc agcttctgct
     6961 ttttgacggt tcaagtcagc tttcttgtct tcagcagttg cttgttcagc agctaatgaa
     7021 gttttcagta cgttcaaatc agcttgttta gataaaagat cgcctttttg agattccaaa
     7081 gcagcttggt tttcagctaa ttcttttagt ttagcatcgt tttcagcttt tttgtcttct
     7141 acagcttttt tatcttgttt ttgttgttcc atcaagtcgt tgtttgcttt taccatcgtt
     7201 gtcattgctt gaacgcgtcc aatagcatct gctaatgaat cagcattcaa tacagcatcg
     7261 atgtagtttg agcttgtgtt gcttacttgt gcttcgcgtg cttgtttttg gattgtatct
     7321 tcacgttttt caatacgttc ttgtaaatct gcaatgtctt ttactaattg tgcagattct
     7381 tgacgcaatg tatcttgttt tgctaatagg tcttgtgctt gtgtgttgat ttcagaaact
     7441 tggctttcta aggcatcgat ttgtgattgt gcatctgctt gttgattttt caagtctgca
     7501 atcttttgat cttgctgttg tatctgagaa tcaaaatcgt ccgccgcggc agcgatcggt
     7561 gatgctacgg cagtcaaggt cattgaacat accattactg ctgatattaa actctttttc
     7621 actcttcatt cctccgactg gcttaattta ataaatatct aaatatattt aagtctttct
     7681 taacgatata aaaattgtac catagcagtg tgtcaacgat ataatagtta tgttacaaaa
     7741 atgtttcaaa acaactttta aaaatgacaa aactgttcgt ttcagaaaat gatcatgaaa
     7801 aagccgcgtt aagtctagca tttcttaaga taacaacgat tctttttcgc tttcgaccta
     7861 aaaataaaaa aagaaattat tcatcactga ataatttctt aaagggaaaa atgaataata
     7921 caaatataat catgttcaac agcagtgtag gtcctaaaaa ccttgtaata aaatacgtgt
     7981 tatttactac tgctaattta aaaatcaact ggaccaccat cgtaaacaat tcataacccg
     8041 tgacaaagat gatcatactg aaaaattcag taaagatgtt gacatggata acgtctttca
     8101 ttacatacat caaccataca attaaaggga gagctactgc atagataccg ataacgccaa
     8161 tatagtaagc gtcatagata gccccgagaa caatcgtggt aatcagtaaa taacgttttt
     8221 caaacgccat actgcaacat aataatgcca atatcagaaa gtgggcattt gacatatagg
     8281 tgcctttcga ccactcccct agcattcttg ttaaatgacc atctatcaac atcaaaaaga
     8341 acaaaacgat tggcagatag tacttcattg tttctttctt caacatggac taatcctccg
     8401 ctagccgttg gacaatcgtc acaactgaca cgtcatacat ttctgcataa ggtttcacat
     8461 aaacttcccg atccagccca taactatctg gtttcgtttt aataaccgta ccgatcggca
     8521 agttagctgg agaattccct ccaagaccgg atgtctggac aacatcccct tctttgatat
     8581 ccgtatcacc agttaattgg gtcaccacta aggcatggag cttttcatca tagtttttaa
     8641 gcaaaccaaa cgcttcgcca ttagccgaag atacccgtac tggaaaatga ttggagcttt
     8701 cattagagga tgacagtaat tcgattttag acgaagccgt attgacctcg atcactcggc
     8761 caatcaatcc tttttgcgac atgactgcca tatttgcttc gataccgtct ttagaaccct
     8821 tgtctacgat cagcatgtcc tgccaactgt caggcgaacg agtaatgaca tttgccgtta
     8881 ctttttcgta actagtcagc gtctgattca actcaagttc ttttttcaac gcttcgattt
     8941 ctctctcgta attcttgttt tgctgtgaaa cgtcatcata gctatcgatt ttttctttta
     9001 gccgctcatt ttcggcataa gttgaaaaaa gattatggac agaatctact ccatttcgta
     9061 cccaccgtac tggggcagag attgttttat caaccattgc aacactgtca ttaacgatgg
     9121 attggaagaa attgggcttt ccttcattcg ctcgctgcgc agcggtcaca cttagtatcg
     9181 tcaccacgat gataacaata accaaggtaa taataatatt tttattagga ttgaacttct
     9241 tcacaaagac acctcg
//
LOCUS       DAZKNO010000110         9178 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000110 DAZKNO010000000
VERSION     DAZKNO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.112"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..2834
                     /locus_tag="ACG8MY_001940"
     CDS             57..2834
                     /locus_tag="ACG8MY_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0431121.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2915..3334
                     /locus_tag="ACG8MY_001941"
     CDS             2915..3334
                     /locus_tag="ACG8MY_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0431122.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3346..3816
                     /locus_tag="ACG8MY_001942"
     CDS             3346..3816
                     /locus_tag="ACG8MY_001942"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0431123.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3833..4612
                     /locus_tag="ACG8MY_001943"
     CDS             3833..4612
                     /locus_tag="ACG8MY_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0431124.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4599..5420
                     /locus_tag="ACG8MY_001944"
     CDS             4599..5420
                     /locus_tag="ACG8MY_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0431125.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5437..6486
                     /locus_tag="ACG8MY_001945"
     CDS             5437..6486
                     /locus_tag="ACG8MY_001945"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0431126.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6499..7503
                     /locus_tag="ACG8MY_001946"
     CDS             6499..7503
                     /locus_tag="ACG8MY_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0431127.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8136..9155
                     /locus_tag="ACG8MY_001947"
     CDS             8136..9155
                     /locus_tag="ACG8MY_001947"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0431128.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3056 a         1537 c         1942 g         2643 t
ORIGIN      
        1 ttctttatta aagattttat tgacaaaatg aatccgtttt ctatgatgga ggaggggtga
       61 ataagttgtt gaagaaggaa attttatgtt acttagagaa ccagacagcc tttttcgatt
      121 tagaacatat gaacgaggtc tttacagcac aaaggttagc tgatcatttt ggggtaaaaa
      181 gaaacacagt cagtcattat ttgaaccagt taaatgatga gcaactacta gtcaaaatca
      241 attctcgacc agtgatttat ttccacaaag aagcttttga aaaacaattt tttaagttac
      301 gcacgaattt ttatttttct atcgatgatc tcaaagagga acagcctttt ttcgcgcaac
      361 caaaagactt attctctttt atgatcggat acgacgccag tttgaaagaa agtatcgaac
      421 aaatcaaaac agctctttac tatccagatg gcggattacc acttttgatt acaggcgaaa
      481 gtggcacagg taaaagttat ttagttaatt tggtctatca gtattgtctg cttcatgatc
      541 tgctagaaga ttctgcacca ttcatcactt ttaattgtgc acaatatgca gataatcctg
      601 aacttttgac tagcaatttg tttggacatg ttaagggagc gtacactggt gcagaagaaa
      661 ataaaaaagg ggcttttgaa gcagcagatg gtgggatctt gtttttagac gaggtacatc
      721 gcttaacacc agaaggtcag gaaaaactgt tcacctatct tgatcaaggt gtgatttatc
      781 gtatgggaga acctaatttg tcacgccgga tcaaaacacg tctctttttt gctacgactg
      841 aagagattac tagtcatttc ttaacaactt tcattcgaag aatacctatc cgaatcgaac
      901 tacctgcact taatcagcgt agtcgtaatg aacggctaga actgatttac tctttttttc
      961 tcgaagaaca aagaaaaatg gggagccctt tgtcagtcag tggaccagct ttgtcacttt
     1021 taacaagtca gaaattatcc ggaaacatag gagagctgaa aaatatcgtc aaagtttcag
     1081 cagcaaaagc atatgctgaa cagcgtgaac agcctgaaat ccatgtcacg atccatcatt
     1141 tgcaaaaaga actacttgcg cagcctatta ctaaaaatac agcacagcaa ggaatctata
     1201 ttacgcaaga ccagacctta gagcaattaa tggagaagca acagcccgaa cagcagcgaa
     1261 tcgttaaaag ttttgagcaa atcctaattg atttcaagaa aaataactgt ctgctatatt
     1321 cttgcgaagg aaaattaaag caagaagtga ttcaattatt tgattttttg ctgtttgaga
     1381 ccagtagaca agaaaaacac gagctgctgg tttattggac acaatctatc cgagagacct
     1441 ttcgacaaat ggaaactgcc tatcaaatca aattcgatgg aaacagtgtc tatgccttga
     1501 gttactatct ctatcagcga agcactatca aatggttacc ggaagaacat gatattatcc
     1561 aactaatcaa agaattagaa caacaagttt ctcagtctta tccatctagt tatcactatg
     1621 tccaacgaat tctggaatta agtaagatca aattagatat tgaagacaca agtatggatc
     1681 ggattttact gacgatctat ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa
     1741 aagcgatgat tgctgctcac ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc
     1801 gtttattaca agaggatcta tttgaatcat ttgatatgcc actagatgtc acaccacagc
     1861 agattgcttc agaaatcctt gattattgcg aaaacagcga tgtttcaaat ggattgatca
     1921 tattagtaga catgggatct ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg
     1981 taccgctttt gattttgaat aatgtgacga cacctttagc aatcacagta ggggaatgcc
     2041 tgcaaaaaaa tgtatcatta gaagagatag cagaagagac tgttcgacag atccagccag
     2101 aatggcgact gctctatcca gaggaaaaca aacccaaagc actcattact acttgcttca
     2161 ctggaatcgg tacggcggtc cacttaagtg aactgctaga aaaaagctta cctacggctt
     2221 gtcagttgaa aatcatccca tatgaatatc agcaattaaa agataagaaa aacagtgacc
     2281 cattattttc catatacgaa gtcgtaggaa tgattggcac cacagatcct gatatcacca
     2341 atatcgacta tctttcatta gaagacctca tctcggggga aaagatgtct gtactagctg
     2401 aatggttgga cactactatg aatgcttcag agaaggaaat attcaatcag cgcatcattc
     2461 gtaatttttc attggaaaaa gtactggatt ccgtgactat tctagataca gagaaggtga
     2521 tgggagaaat cgatcatttt atgagagaat tagaggtgca gttgaatcga agcatcagca
     2581 atcccaaaaa attagcgttg tatgtccatg tgagctgtct cattgaacga ttgatccgac
     2641 aaatgccaat cgaaacctat caaggattag agaaatggaa gcagtgtcaa aaagaagggt
     2701 tatctgctat taaatctgcg tttagtgtca ttgaagagca ttatagtgtc atgatcccca
     2761 attctgagat tgcctatatc tatgatattt tgagtgaaga tacagagttt ctctcgatag
     2821 aagaagaatt ttgaacataa gcaagttgtt gccactaatt ttggcatgca acttgctttt
     2881 ttagtatgtg taagtgaaaa gaggagatga aaaagtggat agagaaatta tcttagcttc
     2941 ccatggaaaa tttgcttcag ggatattaga ttcattagag ctgatctatg ggaaaaatca
     3001 ttctattact gtattcgatt gctatactga tgagaatttt gatttagcgg aaacagtaag
     3061 gcagcttttt atgaggtaca aagataaaga aatcattgtt ctgacggatc tttttggcgg
     3121 aagtgtcaac aacgaattct tacaatatat caatcgggat catgtctatc tcgttgccgg
     3181 attgaatttg ccactattga ttgagctagt atctcggttg gacgctgaag tagagacaac
     3241 tgttttgatc cagcaggtgc taactgattc aaaaaaaatg atccagtttt gtaacgagcg
     3301 gctcaatcag aaaatagaag aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca
     3361 agagtcgacc atcggttatt acacggacaa gtagcttttt catggacaca aacaataggc
     3421 gcagactgta ttttgattgc taatgatgat gtacctacga atgagatacg aaagacaacg
     3481 atcaaattag ccaagccaca aggagtcaaa ctagtgatca aatcaattga tgactcgatt
     3541 gccgcgttga aaagcggggt gacagataaa tataagttat tcatcgtagt ggaatcgatc
     3601 gaggatgcat ataagctggc agaagcgtat ccagaaatca aaaccatcaa cttaggaggg
     3661 atcaaggcaa aacctggtac tcgcagcatc ggaaaagcgg taaatatttt accagaagag
     3721 gaggaacgct tgaaacgatt aattgacaaa caaatagaag tagaaatccg tcaagtacca
     3781 gcagataaaa aagtggatgt aagagaagtt ttgtaagaaa ggaagaacct caatgattgt
     3841 acaagcagtc ttattaggaa ttgttgcatt tgtcgcacaa tcagaatatg cactagggac
     3901 atcgttgctt tctcgtccga ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt
     3961 aaaaactggg atcattatgg gcgcaacttt agagttagca tttatcggat cgttctcagt
     4021 aggtgcttcg attccacctg atgttgtgac cggtgggatt ttagggacgg cttttgctat
     4081 tacagctgga gccggtacag aaaccgcgct tttactaggt ttgccgattg ctacgttgac
     4141 attgatattg aaaaatattt acttagggct tttgattcca attatgaacc ataaagcaga
     4201 caattatgca gaagaaggaa attataaagg agtcgagcat atgcacttgc ttgccggtat
     4261 cggtctttcc ttgatgcttg gtgttgtggt aatggtttct tatatggtgg gaagcaatac
     4321 gatcggaaat ctattaaata tgattcctga cttcgttcaa aaggggttgt cagtagctac
     4381 agggttgatt ccagctcttg gttttgcgat gcttgctcgt ttgctgatca acaaacaagt
     4441 tgcaccatat tttttcttag gttttgctat agcttcttac ttagagattc ctgtgaccgg
     4501 agtagctatt tttggtgcga ttttagcagt tgtggtagtc aatattatga atgtccgtca
     4561 actaccactt caaacaactg caggggaggt agaagaagat gaagaattct gagatcaaaa
     4621 acagcaaaat aacgaaaaaa gagctaaatc acgtattttg gcgttctttt caaatggagt
     4681 tttcttggaa ttatgaacgt cagatgaata tggcttatgt ttatgcaatg attccgattt
     4741 tgaaaaagtt atatcagtcg aaagaagaaa tggcagcagc tttgaaacgg catctagagt
     4801 tttttaacac gaccccacat atcgttacac tgattttagg aatcacggct gcaatggagg
     4861 aagaaaataa ggatgatcct gaatttgatg tgactgcgat cgatagtatc aaaacatctt
     4921 tgatgggacc tcttgcaggg ttaggagatt ctttcttttg gggaactttg cgattgattg
     4981 ccacaggtgt gggtacctct ttagccatgc aaggaaatat tttaggccct atcttgttca
     5041 ttttgatttt caatattccg cacattctgt ttcgctatct agcaacaggt tggggatatc
     5101 gcttaggaac gggttttttg aaaaagatcc aggcaaacgg tatgatggaa agtttgacat
     5161 taggcgcgtc tattattgga ctgatggtcg ttggagcaat gactgccacg atgatcacca
     5221 tcaatattcc tttaaaaatc ggttcaggag aaaatgctgt gactgttcaa agtatttttg
     5281 atgatatcgt tccaaatatc ttatgtcttg gagcatttgg catagtgttt tatctattga
     5341 agaaagaagt gaaaccatta acgatcttga tcggactagc tttctttgga atttttggtt
     5401 cgtggatagg gattttttaa aaaaggagca gagaaaatgt ttacgatgca agattatatc
     5461 tatgaagaaa aggaagtact aagtacgatc ttgaaaaaga atgatttttc gacaagagaa
     5521 catatgaaaa aaacagtgaa tttactgatc ttagcaacgg gttcatctta taacgcttgt
     5581 ttagcagcga agccagcatt ggaatcttat ggtgatctta ctgtcgatat tcaagaacct
     5641 ttttattttt tcaattatgg aaaactttct ccttcaatcg atactgtgat tgctgtgtca
     5701 caaagcggga aaagtgcttc tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta
     5761 cctgttgttg ctatcacaaa tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt
     5821 attgatttag gtgccggggt agaatcagta ggcttcgtta caaaaggcta ttctgcgact
     5881 gttttacagt tactactctt agggatagga attggaataa gtaaaaataa aatcagcaaa
     5941 aaaatagaac aagactacat gcaacagttg cgcaaaataa tcaaccattt accagcgatt
     6001 attcaaaaaa cggaagaatt ttttgatgga tatcaaagtt tatttcgttt agcacagcgt
     6061 ttcgtcacca ttggatatgg tccaaactgg ggaacagcta aagaagcaga gacgaaattg
     6121 actgaaacaa tccgcgttcc gtcacaagga tttgagttag aagcttatat gcacggccca
     6181 tacctggagg cagatgcttc gcatttgcta ttctttattg aaggggaaag tgtaaataag
     6241 gaacgttcgc aaaagttgca aagatatatg agtcggtacg ttggagagac tctcaccatt
     6301 accacaaaaa aagcaagaaa cgagaaaaca cttggattag caatagaatg tgatgaatat
     6361 ctgtctgtat tagcgcttgt cgttccgttt caactattcg cttataaaac cgcagtagct
     6421 aaaggtattg acttgaacaa aaagattttt gaagactttg atacagtctt gaaaagtaaa
     6481 ttataagagg tggaaatcat gctgaaattc aatgaagaag aacaaatcaa agcaataaaa
     6541 ggcgcactag ctttacgtcc gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa
     6601 tttgacgctg tctattatct aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc
     6661 acttatatga atgggaaaag caacttgcct gtctttgtac agcatgctgc agaatattac
     6721 actactggaa acaaaagatt aacaaaagat tctatcgtta ttttgtcctc tgttaccggt
     6781 acaactcaag aagtagtgaa agcagtagaa caaatcaaga aagtaggagc gacactgatt
     6841 ggatttatcg ataaagcgaa cagtaagttg tctcagctat gcgattttgt ggtcacttat
     6901 ccagctcctg gaacagaaca aatcaaattt tttatggcag cagatcgatt gatgtatttg
     6961 aatggagaat tcgaagctta ttcagaatac tatgaacagc tcgaacgtta tttaccaaca
     7021 gggttagtgg aagcagagaa aaaagcagat gcatttggct tatcatttgc agaaaaacat
     7081 cgacgtgata gtatgcatta ttttattgga gcagggaatc aatggggagc tgtttattca
     7141 tatgccatgt gttactggga agaacaaagc tggcttccat cgaaatctat ccatgctgcc
     7201 gagtttcttc acgggacgtt agagatcgta gaagaaacaa ccccagtcac attattttta
     7261 ggcgaagatg aacagagagt gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc
     7321 agcaattata cattgataga cacaaaagac tatccagtag aaggtatcag tgaaaaatat
     7381 cgcggacgtg ttttgtcttt cttattgatg catgtagtga cacaacgcat cgatgctcat
     7441 gtggagcagt tgaactgcca tccattagag atacgccgct actatcgtca gtttgattat
     7501 taggatagac tggataaaaa gaatataaaa aataaagaga gcagcgataa ctgctccctt
     7561 tattttttta aaatattggc atcaattacg ttataaaaca cattttttgc attatctaca
     7621 gttcaactta taatctgttt aatgggacat gtgggtgcca accatttttt gttatgcaat
     7681 agtactattt agttgttgca tggatccgta gtaaaagtcc aagtacaacg agttgcgcca
     7741 gtttacatag cgttttgtat ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt
     7801 caacagagac aatctgtccg ttgcaggacc tgacagattt agaaatgttc aatctgtctc
     7861 ttatgtattt taaatcaata ctcactagga gaagtttcct aacctcctaa tagtaatgct
     7921 ggtagtaaca ttgtcaaaac tgcttcctct taggaacaag caaagcataa cgagtgtaaa
     7981 aataaaatgt agactattgt aacatatcat tgattatttc aagtaaaaac gataaaagaa
     8041 gtaaacaaac acttaaaaac aatggaaatt agtcccaact ttaagaaatg tatactataa
     8101 tggaaattat caattattta tttggaggaa tatccatgaa acaaaaaatc gcagttcttg
     8161 gtcctggatc ttggggcact gctttagcac aaacattggc agaaaacggg catgatgtcc
     8221 gtatttgggg aaatgtacct gaacagattg atgaaatcaa taagtatcat acaaatcagc
     8281 attttttacc tgatttgacg atcccagaat cgattatcgg atataaagaa ttggcagaag
     8341 cagtggataa cgcagatgct attttatttg ttgttccaac aaaagcgatt cgttcagtcg
     8401 cacaggaact tattggtaaa atgaatacta agccaatcat tatacatgca agtaaaggac
     8461 tagaacaaga tacgcataaa cgaatctcag aagttttggc tgaagaaatt ccagaagaaa
     8521 aaagacaggc aattgttgta ttatctggtc ctagccatgc tgaggaagtg gcagtacatg
     8581 atatcacaac aattacagct gcaagtattg atcaaaaagc agcagcctat gtacaaaaac
     8641 tgttcatgaa tgattatttc aggatttata caaataatga tgtgattggc gtagaaactg
     8701 gtgcagcact gaaaaatatc attgcaattg gtgccggcgc tatccatgga ttaggattcg
     8761 gagatgatgc aaaagcagca atcatgactc gaggattggc agaaatcagc cgtcttggtg
     8821 tcgcgatggg tgccaatcct ctgacattta tcggtttgag tggtgtgggg gacttgatcg
     8881 ttacttgtac aagtgttcat tctcgcaatt ggcgtgccgg taacttgtta ggacagggac
     8941 ataagctgga agaagttcta gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa
     9001 aagcagcagt agagctagct caacagctag gtgttgagat gccaattact caaacaatct
     9061 ataatgtttt gtataatgga gaagatatca aaaaagcagc aaaagaaatc atgttacgtg
     9121 acggaaaaat ggagaatgaa ttttctcttc gttaaataga tgatcaatag ttaatcgt
//
LOCUS       DAZKNO010000111         9167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000111 DAZKNO010000000
VERSION     DAZKNO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.113"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..552)
                     /locus_tag="ACG8MY_001948"
     CDS             complement(<1..552)
                     /locus_tag="ACG8MY_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0431129.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILS"
     gene            686..973
                     /locus_tag="ACG8MY_001949"
     CDS             686..973
                     /locus_tag="ACG8MY_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431130.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1389..2927
                     /locus_tag="ACG8MY_001950"
     CDS             1389..2927
                     /locus_tag="ACG8MY_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0431131.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2924..3889
                     /locus_tag="ACG8MY_001951"
     CDS             2924..3889
                     /locus_tag="ACG8MY_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431132.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3876..5198
                     /locus_tag="ACG8MY_001952"
     CDS             3876..5198
                     /locus_tag="ACG8MY_001952"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0431133.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5261..5365
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5439..6182
                     /locus_tag="ACG8MY_001953"
     CDS             5439..6182
                     /locus_tag="ACG8MY_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0431134.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAV
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6197..7189
                     /locus_tag="ACG8MY_001954"
     CDS             6197..7189
                     /locus_tag="ACG8MY_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0431135.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7201..7938
                     /locus_tag="ACG8MY_001955"
     CDS             7201..7938
                     /locus_tag="ACG8MY_001955"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431136.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7928..8650
                     /locus_tag="ACG8MY_001956"
     CDS             7928..8650
                     /locus_tag="ACG8MY_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0431137.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8734..>9167)
                     /locus_tag="ACG8MY_001957"
     CDS             complement(8734..>9167)
                     /locus_tag="ACG8MY_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0431138.1"
                     /translation="INLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETV
                     QTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLD
                     SSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3003 a         1581 c         1942 g         2641 t
ORIGIN      
        1 agatagaata gaacagctaa gtaatgtata cagaaaatgt gtgagatcca aataaagcaa
       61 ggagacaact aaaataccaa aagtaatcaa gaaaagactt ttgccaatct taatatgaaa
      121 atatttttca atggcttgat aaatgatatc tgttccgcct gtgcttccgc cgaatcgaaa
      181 gacgagaccg caaccaatgc cagaaagaat acctgtcatc aaactattga tcagtagact
      241 atctacagca aaatgtatat gaaggctctc ccataaatgt aaaaagaaaa ctagcgcaaa
      301 tgttccgacg atcgtccgga gaaaagcgtg ttttccaaaa agaaacaatg agataagcaa
      361 taaaggtaaa ttaaaaagca gtgaagtgac agaaggagcg aatccaaaaa gattatgcaa
      421 aagcaaagca ataccagtaa ccccaccatc tgccagctta gatggaatat tgattcctgt
      481 cacagctaat ccgtagattc ctgcgcctaa aatgattccg cacaattctg taaataaatt
      541 taattttttc atttctagcc tcttttcttt tgaaaatttt cagcaacttc tttattatac
      601 tgacttatac tcttacttca agaggaaaat gaaaaaaaat tatttttggc tgtttctttt
      661 tatttatcaa ataaggagtg gaaaaatgcg agcagcagac ttattgacaa ttcttacaaa
      721 atcacccaaa aaaacagagt atcaaggagt ttttttggct caagaacaaa aactggtgcc
      781 tgtgacaatg gtccagataa ataaagcaaa tcaatttatc tttttatttg aacacggcaa
      841 agcaccactt gctatgaaag acatctttct ttttttgatg aagaaccgtg agaaagctgt
      901 ttattatcag tcaaaagaag aactcattcc tctttatgga gtgaaagaga tagaaaataa
      961 agtggtgata taagtaaaaa taagaaaaca agacgtttat gatgtcaaaa caaccatttt
     1021 ttacattttt ggcaaaaaag tgtcatgata cagtataata ttcgttgaac aggccaataa
     1081 aggtgttctc ccccacgaga tttctacttt attggctgtt tctttttatt aaaataaaag
     1141 ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag atttttatgg
     1201 tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct tggattcttt
     1261 ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt aaggttaagg
     1321 caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat aacagaaagg
     1381 aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag ttgctggaag
     1441 ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca ctgattgcta
     1501 ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta ttgcagatca
     1561 cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc ccatttgtag
     1621 ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt ccgattttcc
     1681 aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca gcggtagcat
     1741 ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca ttacctgtca
     1801 ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact ttggcagcat
     1861 tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga aatacacctg
     1921 ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc agcggactga
     1981 ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca gcagtggtgt
     2041 gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa accatcacga
     2101 ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa gaattgattg
     2161 aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg agttttttgt
     2221 tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt actgtcctag
     2281 aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa gaaaaaacat
     2341 atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta aaaccaatgg
     2401 cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa catcaactta
     2461 aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc acactggaat
     2521 ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg aatttttcaa
     2581 gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga gatgtcaata
     2641 ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg actctagaag
     2701 atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa gattttgcta
     2761 aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata acagaggatc
     2821 aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca gaagtagcac
     2881 gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta aattaatcga
     2941 gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc taaatctggc
     3001 aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa aaacaactac
     3061 tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact tcaaaggaaa
     3121 atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg tgctgcagca
     3181 aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc ctgaaatgtt
     3241 aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa aagttggctt
     3301 atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag aacagcaacg
     3361 gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg acgagccatt
     3421 tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt ccttacacga
     3481 agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa tcaagctagg
     3541 tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac cagagcagtt
     3601 attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga aacagacgaa
     3661 agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa taccagaaaa
     3721 tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc ttctttcggt
     3781 gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga caagtaaaga
     3841 aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat gcaatcatca
     3901 tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca ggtaaaacag
     3961 tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga tgtgatccta
     4021 aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg agtggaaaag
     4081 gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa cgaacattta
     4141 cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc gatcatcttt
     4201 caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa gtcttccatg
     4261 cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg gagggaaagg
     4321 aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa gaaatcattt
     4381 ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct gcagggagca
     4441 aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg tctttggtag
     4501 aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt gttgaaaccc
     4561 aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg gaattagtag
     4621 cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta gcacttgagc
     4681 aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg caaacgagca
     4741 atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa ttgacaccag
     4801 tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca agcgaaccta
     4861 tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct gaagtaggag
     4921 ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt accagttggt
     4981 tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt gaccagcaaa
     5041 attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc gatgatttga
     5101 cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc atggggtatc
     5161 cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa ctaaagcgct
     5221 tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc tggggatgtc
     5281 gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag ttaatactgg
     5341 tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc atttcaagcg
     5401 ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat ctttatccag
     5461 cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt tggaaaatac
     5521 ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggta gatgcacaag
     5581 tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg gtaatagcta
     5641 gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta gaaatgtctt
     5701 tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg ccattactta
     5761 ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg ataagctatt
     5821 ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag caaatcatct
     5881 ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa ataccagcgg
     5941 gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc agcggagcag
     6001 tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata cgcgtaaaaa
     6061 acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta ttgagtttat
     6121 tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt tatatttttt
     6181 aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt tgcttttatc
     6241 cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt tgaaaaaagt
     6301 gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc tagcaaaaga
     6361 aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag gagataattc
     6421 gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt atcaagaaga
     6481 tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg ctacagtgat
     6541 caaacataat acttctggat tcgccaccct atcagacagt aacatccatt caccaaaaga
     6601 ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg tcttaaaagc
     6661 tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa gcaatggaga
     6721 agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag gttgggatat
     6781 gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag aattagatga
     6841 acggttagat tactatactc cggtaattat tacgaatgat caactgatca aaagtgatcc
     6901 agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag ctattaagga
     6961 tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc ggacgttttt
     7021 agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga attggggatt
     7081 gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg gattgatcaa
     7141 gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg agtgatagat
     7201 atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat attagaagac
     7261 atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag cggagccggt
     7321 aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg aacgatcaaa
     7381 gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc gcaagaagat
     7441 atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa aatcaatcat
     7501 ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt aaacggctat
     7561 gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc cttgttacga
     7621 acaatcatga atccttcttc ttacttgctt cttgatgaac catttggagc gttagatgct
     7681 atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc aaagcggact
     7741 actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag gattcttgtg
     7801 ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa acgacggtca
     7861 cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt attggcaggt
     7921 gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt tagcactcct
     7981 tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag aatggaagga
     8041 aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc atccttggaa
     8101 agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa ggatcatcgg
     8161 tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa aaaaagtatt
     8221 tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc acacaaaagg
     8281 atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt tgatccattg
     8341 gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt tcagcattgg
     8401 tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc caatcgacca
     8461 cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg ggattttttc
     8521 agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg agatcaaaga
     8581 gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt tcattagatt
     8641 aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg ctttctttgt
     8701 ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg cttcccagaa
     8761 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
     8821 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacgtccgat
     8881 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
     8941 tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
     9001 ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa caggttgttc
     9061 agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt gtgcagagcc
     9121 ttctgttggg attgttaatt gttgtcctga gtaaatcagg ttaatat
//
LOCUS       DAZKNO010000112         8991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000112 DAZKNO010000000
VERSION     DAZKNO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.114"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            165..1211
                     /gene="gmd"
                     /locus_tag="ACG8MY_001958"
     CDS             165..1211
                     /gene="gmd"
                     /locus_tag="ACG8MY_001958"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFQ0431139.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1219..2304
                     /locus_tag="ACG8MY_001959"
     CDS             1219..2304
                     /locus_tag="ACG8MY_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0431140.1"
                     /translation="MNKHICFYLSSHGFGHIARNLPIIMATLCRCEAKITLVCGANHL
                     DFAKRNLEQELPKELIDRVRYRPINTDIGLINREGTLLVDTGKLTEICEYFLAKVPSL
                     AKTEAEWLKREAVSAVLCDMPIWAMDACDMAGVPFLYVGNFTWTELYREFLPEHIWKE
                     YAKRYGKIQHTMLYALNNEEMLEFLQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVF
                     VALGMSARFKQPIDVSHAPYDFITTLGVPLVGENVTVISNSTPNTQDYIAAADYVITK
                     AGWGTVAECILAQRPMALFARDTVLEDRTTICKLQKLGLAISVTPENLHRVERVIEDL
                     KQLDTSNFDTFYDAASEIADKLISLMQ"
     gene            2341..3285
                     /locus_tag="ACG8MY_001960"
     CDS             2341..3285
                     /locus_tag="ACG8MY_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFQ0431141.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3353..4666
                     /locus_tag="ACG8MY_001961"
     CDS             3353..4666
                     /locus_tag="ACG8MY_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFQ0431142.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4858..5652
                     /locus_tag="ACG8MY_001962"
     CDS             4858..5652
                     /locus_tag="ACG8MY_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HFQ0431143.1"
                     /translation="MTLTEVLQEHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPT
                     DEYAIEKENGQLGKTEMWYVLDASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKK
                     NDVFFIEAGTIHAIGAGALIAEIQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVAN
                     LKGLSEPKQPLRVLKYRAGVAAELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVL
                     LCVNGCGTVRFDNESLDIYKGDCLFVPADSTEISIHGSIQFLDVRG"
     gene            5657..7309
                     /locus_tag="ACG8MY_001963"
     CDS             5657..7309
                     /locus_tag="ACG8MY_001963"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0431144.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7333..8199
                     /locus_tag="ACG8MY_001964"
     CDS             7333..8199
                     /locus_tag="ACG8MY_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0431145.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            8388..8513
                     /locus_tag="ACG8MY_001965"
     CDS             8388..8513
                     /locus_tag="ACG8MY_001965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431146.1"
                     /translation="MAGITGVNVIVTYNHHLPIQCLDFSCNYYYIELVIPQKSAS"
     gene            8591..>8991
                     /locus_tag="ACG8MY_001966"
     CDS             8591..>8991
                     /locus_tag="ACG8MY_001966"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431147.1"
                     /translation="MEEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSN
                     LWFRQEISIAEIEDAVLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKN
                     ISYLKVYTATPPKKPTPPRFQSNGWSLFFSH"
BASE COUNT         2442 a         1874 c         2275 g         2400 t
ORIGIN      
        1 aaaaaaactg ttgccattcc aattcacgaa aaactaatat tgtgctaatg cgtgcgtaga
       61 ttgttctatg aaatatcgaa aattatcgtg tattcttgaa aagtattaga aattacgagc
      121 catttatatg gccttatcac ttacatagga aaggataatg tatcatgaaa aaagcgctga
      181 ttactggtat tactggtcag gacggttcct atctggcaga tttcctgcta gagaagggct
      241 acgaagttca cggtatcact cgccgtgctt ctatttccaa taccgctcgc attgaccatt
      301 tgatgggcaa gatcaccctt catgacggtg acctgtccga ctcatcttct ctgattcgta
      361 tcatcagcat tgttcagcct gatgaaatct ataacctcgc tgcacagagc catgttcagg
      421 tttcttttga tgttcctgag tactccggcg atgttgatgc tttgggcgtt atgcgtattc
      481 tggaagcttg ccgtattctg ggcctgacga agaagactaa gatttatcag gcatctactt
      541 ctgagctgta tggcaaggtt gaagaagttc ctcagcgtga aaccactccg tttcatcctt
      601 acagccctta tgctgttgca aagcagtatg gcttctggat cactaaggaa tatcgtgaag
      661 cttatggcat gttcgctgtc aacggtattc tgttcaacca tgaatccgaa cgtcgcggtg
      721 agaatttcgt cacccgtaag atcaccctcg ctgccggccg cattgcagaa ggcctgcagg
      781 accatctgga actgggcaac atggactccc tgcgtgactg gggctatgca aaggactatg
      841 ttgagtgcat gtggatgatt ctccagcatg agactcccga ggacttcgtg atcgctaccg
      901 gcgagcagca cactgttcgt gacttcaccg agaaggcatt cgcagcaaat ggcatgacca
      961 ttcgttggga aggcactggc attgatgaaa agggctacga tgttgaaacc ggcaagatgc
     1021 tcgtttgtgt aaatccacag tggttccgtc cgactgacgt tgacaacctg tggggcgacc
     1081 cgactaaggc aaagaccgtt ctcggttgga atccgcagaa gacctcttac gaggaactgg
     1141 ttaacatcat ggcaaagcat gaccgtgaat tggcaaagcg cgaagctgca atgaagaacg
     1201 cagtcaagta agctgacatt gaataaacat atctgtttct atctttccag tcacggcttc
     1261 ggacatatcg cgcggaatct gcccattatt atggcaacac tctgccgatg cgaagcgaag
     1321 attacactgg tttgcggcgc aaatcatctg gattttgcaa agcgcaattt ggagcaggaa
     1381 ctgcccaaag agctgataga ccgcgtgcgc tatcgtccaa taaacacaga tatcggtctg
     1441 attaaccgtg agggcaccct actggtggac acgggtaagc tgaccgagat ctgtgaatat
     1501 tttttggcaa aggttccttc tttggcaaag acagaagccg aatggctcaa gcgagaagct
     1561 gtcagcgcag tgctttgcga tatgccaatc tgggcaatgg atgcctgcga tatggcagga
     1621 gtacctttcc tgtatgtagg aaacttcact tggacagagc tgtatcgcga atttttaccc
     1681 gaacatattt ggaaagaata tgccaagcgt tacggtaaaa ttcagcacac catgctttat
     1741 gcgctaaata acgaagaaat gttggaattt ctccaaaaca gcgaaaaaac accgacttcc
     1801 gttgtggcgc gtccgattca taacgacgaa gtgcaacgca ttaaatcaca gcatgaaaaa
     1861 ccgcctgtat ttgtcgcact cggtatgtcc gcgcggttca aacagccgat tgatgtcagt
     1921 cacgcgccgt atgatttcat tacaacgcta ggtgttccgc ttgtgggaga aaacgtaacc
     1981 gttatttcta acagcacccc caatacacag gactatatag cggcggcaga ttacgttatt
     2041 acaaaggctg gatggggaac tgttgcggag tgtattctcg cacagcgtcc gatggcactg
     2101 ttcgcccgcg atacagtttt ggaagataga acaactattt gtaaattgca aaaactgggt
     2161 ctggcaattt ccgtcacacc ggaaaacctc caccgcgtag agcgggtgat tgaggacctc
     2221 aaacagctag acacctcaaa ttttgataca ttttatgatg ctgcgagcga aatcgcagac
     2281 aaattgattt ctctcatgca atgagagatg ctctaaattt agaaaggagc aagcattatt
     2341 atgatggaaa aagacgcaaa aatctacgta gccggtcatc gtggcatggc tggttccgca
     2401 atcgtgcgcg agctgaaccg tcaggactac aacaatatta tcacccgcac ccataaggag
     2461 cttgacctgt gccgtcagga cgctgttgaa gcatttttcg cacaggaaaa gccggattat
     2521 gtatttctcg ctgctgcaaa ggttggcggt attatcgcaa accagaacgc tctggcagac
     2581 ttcatgtatg aaaacatgat tctggaaatg aacgtcatca actccgcatg ggagaatggc
     2641 tgtaagaagt tacagttcct cggttcttcc tgtatctatc cgcgtatggc accgcagccg
     2701 atgccggaaa gctgtctgct gacaagtgag ctggaaaaga ccaacgaggc ttatgcactg
     2761 gcaaagattt ctggcctgaa gtactgtgag tttctaaaca agcagtacgg tactgactat
     2821 atctctgtta tgcctactaa cctgtatgga cctaacgaca actatcatcc tactcacagt
     2881 catgtgctcc ccgctttgat tcgtcgcttc catgaggcta aggaagcagg tctgcctact
     2941 gttacctgct ggggtgatgg ttctcctctg cgcgaattcc tatatgttga cgatctggca
     3001 aacctgtgtg tgttcctgat gaataactat agcggtgacg agactgttaa tgcaggtact
     3061 ggcaaggaac tgtctatcaa ggaactgacc gagatggtag caaaggttat tggttacgaa
     3121 ggcgagattc tgtgggatac cagcaagccg aacggtacac ctcgtaagct gctggatgtt
     3181 tccaaggcaa ccaagctggg atggacatat aagactgagt tggaagatgg catccgtctg
     3241 tcctacgagg acttcctgaa taacccgatg cgtgctgaac gctaatttac cgaggttgct
     3301 gtgatttttg aggattgcag cagcctcttt cttataaaaa ggagagatca ttatgaatat
     3361 tgttttactg tctggcggtt ctggtaagcg cctttggccg ctgtctaatg acatccgttc
     3421 caagcagttc attaagattt tcaaaactga agatggatat gaatccatgg ttcagcgtgt
     3481 ctaccgccag attaagaagg tagataacga cgcaactgtt acgattgcaa cctctaagac
     3541 acaggtttct gctatccata accaacttgg tgaggatgtg ggtgtatctg tagagccttg
     3601 tcgccgcgat accttcccgg caatcgcatt ggctaccgct tatctggtag atgtgatggg
     3661 cattgatcca gaagagtccg tagttgtttg cccggttgac ccttacgtag aagatgacta
     3721 ttttgaggcc ctgaaatctc tgtccgatca ggctgacaaa ggcgaagcta atctggtgct
     3781 catgggtatt gaaccgactt atccttccga gaagtacggc tacatcattc ctgagagcgc
     3841 agagcagact gctttcgtaa ataccttcaa ggaaaaacct tctgaggctg tggcaaagga
     3901 atatatcgca cagggtgcac tgtggaacgg tggtgtgttt gtctacaagc tgaagtatgt
     3961 catggagcgt gcacatcagc tgattgactt tgtagattat caggatctgt tcaacaaata
     4021 cgatactctg actaagattt ccttcgacta tgccgttgtt gagcatgagg acaagattca
     4081 ggtacagcgt tttgctggta tgtggaagga tctggggact tggaatactc tgaccgaagg
     4141 tatggaggaa tccattatcg gtaaaggtgt tatgaatgag aattgcgatg gcgtacacat
     4201 cctgaacgaa ctggatgttc ctgtgttggc tatgggtctg catgatgtgg tcatctgtgc
     4261 atctcgggaa ggtattctgg tttccgataa ggaacagtcc agctatatta agcctttcgt
     4321 tgacaagttc gagcagcaga tcatgttcgc agagaagtct tggggtagtt ataaggttat
     4381 ggatgtagaa gcagagagca tgaccattaa ggttactctg aatgctagtc actccatgaa
     4441 ttatcacagc cacaagaacc gtgacgaggt gtgggtggtt ctggccggta ctggtaagac
     4501 catcgtggat ggatatgagc aggttgttaa ggctggcgat gtgatcacca tgagtgctgg
     4561 ctgtcggcat accattatgg cagatactga gctgaagctg attgaagtac agcttggcaa
     4621 ggatatcaat gttaaagata aacagaaatt tgagttagag tattaaaaaa cggaggaggg
     4681 ctcgttatac agaaaccgtt tttattgaag ccttccgcaa aggattatct gtggggggga
     4741 accgcctgaa tgatgaattt gcaaaaggaa ttgagatgtc tcctcttgcg gaaacttgga
     4801 atgctctacg cacccggatg gaccgagctt tgttgtgtct ggtcagagta tcaggggatg
     4861 accttgacgg aggtgttgca agaacaccca gagtttctgg gaacgcatcc gcagaccaaa
     4921 ggagaattgc cgatcctgat caaattcatt gatgcgaaaa aggatttgtc tgttcaggta
     4981 catcccacag atgaatatgc aattgaaaaa gaaaacgggc agcttggaaa gacagagatg
     5041 tggtatgtac tggatgccag cttaatctat gggctgcaga aaataacaga agaagctgtg
     5101 atccgtgaag cgattgctca gggaactgtt gagcgttatc ttcagaaggt tccggtcaaa
     5161 aagaatgatg tgttctttat tgaggcggga acgattcatg cgattggtgc gggtgcacta
     5221 attgcagaaa ttcaggaaaa ttccaatctg acttatcgtc tctacgatta tgaccgtgtt
     5281 gacaaaaatg gtaaaaagag agaacttcat gtagacaaag cgctggaagt agcgaacttg
     5341 aaagggttgt cagaaccgaa acagccgctt cgtgttctaa aatatcgtgc tggtgtagct
     5401 gcggaattgc tttgcagatg caagcatttc gaggtctctc gtatgattgt caacactgag
     5461 agaagacaga cggtacacta tcgtgcggat tcaatcagct tccgtgtgtt gctgtgtgtc
     5521 aatggctgtg gtaccgttcg atttgataat gaatcgttgg acatctataa gggggactgc
     5581 ctttttgtgc cggccgattc tactgaaatc agcattcacg ggtcgattca gtttctggat
     5641 gtgagaggat aataatatga attataaagt agaatatgag cgttggctga atcatgccaa
     5701 tgcagatatt atagaagaac tgcgttccta ttctgagaag gacattgagg actctttcta
     5761 taaaaatttg tctttcggta ctggtggtct gcgtggtacc atcggtgcag gcactaatcg
     5821 catgaacgta cacaccgtag gtaaggcaag ccaaggtctg gcagactatc tgaataagaa
     5881 ctatgaagcc gcttccgtgg tggttggcta tgattcccgt atcaaatctg atgtgtttgc
     5941 aaagactgcc gcagacatct ttgctgcaaa tggtattcag gtgcacttgt ggccagaact
     6001 gcttcctgtt cctacagtat cttttgcgac tcgttatctg aaggcagatg ccggtgttat
     6061 gattacggca tctcataacc cttctaaata taacggctac aaggtatacg gtgcagacgg
     6121 ctgtcagatt actaccgagg ctgctactgc aattctggca gaaatcgaaa agctggacat
     6181 ctttgctgat gtaaagatgg atggcgatgc ttctaagatt gcatccattc agtcggaagt
     6241 tctgactgct ttcatcgaag aagtgaaggg gcagtccgtc ctgtttggcg aagaagtgga
     6301 caagaatgtt gctatcgtgt attctcctct gaatggtacc ggtctggtgc ctgttacccg
     6361 caccctggca gagatgggat acagcaatgt gaccgttgtg gaagaacagc gtctgccgga
     6421 cggtaatttc ccaacttgtc cttatccgaa ccctgaaatt aaggaagcta tgtctctggg
     6481 tatggagtat gccaagaagt acaatgcgga tctgctgttg gccactgacc ctgactgcga
     6541 ccgtgttggt atcgcagtta agagcgcaca tggtgactat gttctgttgt ctggtaatga
     6601 gactggtctg ctacttctgg actacatctg ttctcagaga attaagcatg gaaaaatgcc
     6661 tgctgaccct gtgatggtca agaccatcgt caccatggat ttgggtgaac gtatcgcatc
     6721 caactatggt gttcgtacca tcgatgtgct gaccggcttt aagtttatcg gtgagcagat
     6781 tggtatgttg gaaaaggctg gtaaggttga cagctatatc ttcggctttg aggaatctta
     6841 cggctatctg tccggtagct atgtccgtga caaggacggt gttaacgctg cttatctaat
     6901 ctgtgaaatg ttctcttact acaagactca gggtatcagc ctgctggata agctgaacga
     6961 gatttataca aagtatggct attgtttgaa caccctgcat tccttcgagt ttgacggtgc
     7021 ggcaggtttt accaagatgc aggaaattat ggttgaattc cacaagggtc tcgatataat
     7081 tggtggtaag aaggttattg aaactttaga ctacagtaag ggcctgaatg gtctgccgaa
     7141 gtctgatgtt ttgaagttta tgctggaaga taactgctct gttgtggttc gtccttctgg
     7201 tactgagccg aagctgaaga cctatatcag cgtcagcgca gagaacaagg aagccgccga
     7261 agctatggaa gctattatgg ctgctgaact ggcggaaatg ttcgagtaag aaaagaaagg
     7321 agaaatcttg taatggagga actgttttct aacgctttga aatcggtgaa gtcaaatcgt
     7381 gtgctgtaca cggcatcgtt ctttgcaaga tcctctcttt tgtatttgca ggaaattgga
     7441 cagctgactg ctctgaaggc acatacatct aggcgtacag gattgtcatc ttacctttgt
     7501 ttcctggtag aagttggttc aggcacattg gagtataagg ggcagaaata tacgcttcaa
     7561 gtaggagata tggtgtttat tgactgtttg aagccgtatg tccatgagtc atcagaagat
     7621 gatttgtgga ctttgcggtg gtgccatttt aatggcattg gtatgacgga agtctacaaa
     7681 aaataccttg aacgaggtgg tcaaccggtc tttaaaacat caaaaacaga taagtatgct
     7741 gacttgctga ggagccttca tatggtggca gagggacagg agtatgtccg ggatatgaaa
     7801 atcaatgttc tactcagtga actgctgatg catttgatgg aagattcttg gaatcctgac
     7861 aaagtgccga atttctctac aaaaaaaatg gaattgcaga acataaaaca atatctggat
     7921 gaccattaca cggagaagat tacgctggat cagttgagtg cagagttttt tatcaataaa
     7981 cagtacttag ctaagatttt tcgtgaaact tacggtacaa ctgttgtcgg ttatctgaat
     8041 cagaaacgaa tcacacaggc caagcacttg ctacgcttta caaatctaag tatggaagag
     8101 attgcaacaa aaattggcat aacagatgca aactattttt ccagaacttt tttcaaggtg
     8161 gaaggtgttc gtcccagcga attccgcaag atgtggtaat aaaaagccgg cggtgttgag
     8221 ccgccggtca gtacctgtcg ttaacccgca ttcaaagctt taatctcagc tttgatttca
     8281 gcacgaacac gatagtaatc ttcagtttcg atttcggtat ccagccgagt tgctgtcttt
     8341 tcgcagtcag gacaacgctc tggctgttcg tcggcaggga agctgtagtg gcaggaatca
     8401 caggtgtaaa tgtgatcgtc acgtataatc atcacctacc aatccagtgc ttagattttt
     8461 catgcaatta ctattatatc gaattggtga ttccacaaaa atccgcttct tgataaatgt
     8521 acgattcaga gtgatatata gtactgtact gccaaaggga ataccacaac ggcagaaatg
     8581 gatgattcac atggaagaaa ttatgcagat gattttaggg aacgcaaaat tccagctcgg
     8641 acaggtggtt tcgacccctg ctgtattgga aactttcaac cccctgcaga ccgcaaacgg
     8701 cattcccgaa gccttgagca acctttggtt tagacaggaa attagtattg cggagatcga
     8761 ggacgcagtt ctggatgcca agaacggcaa ggagctagca aacagcctca accgcatcaa
     8821 gcttttccgc aagttcaccc tcgaccgaga gctggacggc atggtcagac tgaagggcac
     8881 ggacgtctgg aagaacatca gctacctcaa ggtctacacc gccacgccgc ctaagaaacc
     8941 gaccccccca aggttccagt cgaacggctg gagccttttc ttctctcata a
//
LOCUS       DAZKNO010000113         8984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000113 DAZKNO010000000
VERSION     DAZKNO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.115"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(15..1154)
                     /locus_tag="ACG8MY_001967"
     CDS             complement(15..1154)
                     /locus_tag="ACG8MY_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0431148.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACG8MY_001968"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACG8MY_001968"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0431149.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACG8MY_001969"
     CDS             complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACG8MY_001969"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0431150.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACG8MY_001970"
     CDS             complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACG8MY_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0431151.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACG8MY_001971"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACG8MY_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0431152.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7259)
                     /locus_tag="ACG8MY_001972"
     CDS             complement(6168..7259)
                     /locus_tag="ACG8MY_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0431153.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACG8MY_001973"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACG8MY_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0431154.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="ACG8MY_001974"
     tRNA            8802..8873
                     /locus_tag="ACG8MY_001974"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
BASE COUNT         2330 a         1895 c         1659 g         3100 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgtccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacactt gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc ttttgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttga tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc caccacctgt
     3901 tgagatgtgt gtgaatttgt cagcgaatcc taattgttct gctgctgcag ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttct actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat taagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattgggcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaaa cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga ggaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag ttttgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc cgacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaaatctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt ggaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aaagagaatt ggtggacagt tttttgttgt atctgtatag atat
//
LOCUS       DAZKNO010000114         8762 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000114 DAZKNO010000000
VERSION     DAZKNO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.116"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            26..1087
                     /locus_tag="ACG8MY_001975"
     CDS             26..1087
                     /locus_tag="ACG8MY_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0431155.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1090..1986
                     /gene="murQ"
                     /locus_tag="ACG8MY_001976"
     CDS             1090..1986
                     /gene="murQ"
                     /locus_tag="ACG8MY_001976"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0431156.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2000..3982
                     /locus_tag="ACG8MY_001977"
     CDS             2000..3982
                     /locus_tag="ACG8MY_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0431157.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSAATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4047..4493)
                     /gene="nrdI"
                     /locus_tag="ACG8MY_001978"
     CDS             complement(4047..4493)
                     /gene="nrdI"
                     /locus_tag="ACG8MY_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0431158.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4704..6128
                     /locus_tag="ACG8MY_001979"
     CDS             4704..6128
                     /locus_tag="ACG8MY_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0431159.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6309..7082)
                     /locus_tag="ACG8MY_001980"
     CDS             complement(6309..7082)
                     /locus_tag="ACG8MY_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0431160.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7079..7984)
                     /locus_tag="ACG8MY_001981"
     CDS             complement(7079..7984)
                     /locus_tag="ACG8MY_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431161.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8138..8458)
                     /locus_tag="ACG8MY_001982"
     CDS             complement(8138..8458)
                     /locus_tag="ACG8MY_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0431162.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2747 a         1579 c         1869 g         2567 t
ORIGIN      
        1 acggatacag aaaaagaggg ataatatgtt tggcttttca atatttatga acgattcttt
       61 gtcagaagag aagaaacagt atatccgcca aatggcatcc aatggctttg tcggtatttt
      121 cacttccatg catataccag aagatgatgt ttcggcatat caaaagcgat tgatcgaact
      181 aggaagcgaa gcgaaaaaga ataaactaga attgatggtc gatatttcag gagaggcatt
      241 ggaacgagcg ggtttttcat tagaagatct aacgcctttg aaagcaattg gtgtaacagg
      301 tttaagaatg gattatcata tttccaatca gcagattgcc gactggtcac atcaattgaa
      361 aatcagtttg aatgctagca cgatcacgcc taaagatatc gatgaattga aagaagcaga
      421 agctgatttc tcacagattg aagcttggca taattattat ccacgtcccg aaacagggct
      481 ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa attcaaggtc tccttgtcca
      541 aggttttgta ccaggagata cagagttgag aggcccacta tatcaaggat tgccaacttt
      601 ggaagaacat cgagggatcc atcctcttgc aggcgctcta gatctattag caagtaacac
      661 ggatattgtt tacatcggag atgcaggtct gtcagaaaac gttcaggagc aatttgcttc
      721 tttccaaaaa gaacagacag tcttgctcca tacggaacct gtagatgaag aattttacga
      781 atatatatta gggaagcata cgaatcggca agacgatgca agagatgtca tacgaagcgc
      841 agatgcacgt tttcgagaga ttccgcctat cccagcaaga aatacagcta cgcgtatgaa
      901 agggtctatt actttagata acgaaaagta tttgcgctat atgggtgaga tccaattaac
      961 caaatatgat ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga
     1021 gctaccattg atcaaccaaa tcaaagctgg tatgaactat caatttataa gaaaagaagg
     1081 aagataaaat tgagcaaaat caatttagag aacctaacaa cggaacgcag aaaccaagaa
     1141 acttttggat tagatgagat gagtatttct caagccgttc aactaatgaa caaagaagat
     1201 cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac
     1261 acgattgagt cctttaaaaa aggtggacgt ctgatttata tgggagccgg aacaagtgga
     1321 agactaggtg tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga accggaaatg
     1381 gtcataggtc taattgctgg tggagaagaa gcaatgactg tagctgtcga aggggccgaa
     1441 gattcttatg agctagggca agaagatcta atcaagctga atttgacaaa aaatgatata
     1501 gttattggta tcgcagctag cggtcgtaca ccatatgtga tcggtggatt agattacgca
     1561 aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata aacaagccga aatcagtaaa
     1621 cacgcggatt tcccaatcga agtcgatgca ggtccagaat ttttgacagg ctctactcga
     1681 ttgaagtcag gaacagcaca aaaacttatc ttgaatatga tttctactat cagtatgatt
     1741 ggtataggta aagtattcaa taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta
     1801 atcgaacgct caaaacgaat catcatgcaa gcgacagaag ctgattacga aacagctgaa
     1861 aagtactttg aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat
     1921 agtgataaag aggaagctca agcaaaatta gctgaagcaa acggatttgt aaaagggacc
     1981 ttatgagggg gaaaagacca tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca
     2041 aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc attgtccact gcatgactcg
     2101 tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc tctgaattaa aagcgattcc
     2161 aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc atcatcggac ctgggaaagt
     2221 gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg aaattaggag aaaaactttc
     2281 ttctgtaagt gcagctacag caaattcatc agcatctgga aaagaccgtg taaacgaacg
     2341 ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt
     2401 attaaaagac atttctaata tctttgtgcc aatgattcct gcatttgtcg gtactgggat
     2461 cgtggcaggg attgccgcag ttttagcaaa cttagtcaca gccggaacac ttgatgcagc
     2521 aacatggcaa caatatatcg atgtgatgaa catcttgaaa aacgggatgt ttagctacct
     2581 agttatctat acaggtatca atgctgcaca agtttttggt gcaacaccaa ctcttggtgg
     2641 agttatcggg gccgttgtga tgctgacagg gatgaatccg gaagcaccac tgaaaaactt
     2701 atttactggt gaagctcttg cagctggaca aggtgggatc ctaggtgtca tcttcgctgt
     2761 ttggctattg tcaatcgttg agaaaaaact gcataaagta gtacctgatg cggtagatat
     2821 catcgttaca ccaactcttt cattgattgc catcggtctg attgaaattt tcttcatcat
     2881 gcctttagca ggtttcattt ctgatggcat ggtcggtggg atcaactggg tacttggcgt
     2941 tggtggagca tttgccggct tcgttctagg cacattattc ttgccgatgg ttatgttcgg
     3001 actgcatcaa attttgactc caatccatgt gcaaatgatt gatgaaactg gtcgtacgct
     3061 attgcttcca attcttgcga tggctggtgc aggacaagtg ggtgcagcat tggcattatg
     3121 gattcgctgc aaaaaagata aagaattaac agaaatgatc aaaggagctt taccagtcgg
     3181 gatcctagga atcggcgaac cattgattta cggagtaaca ttacctctag gacgcccatt
     3241 tattactgct tgtatcggtg gaggtatcgg cggggcagtg atcggaatga tcggaaatgt
     3301 cggggctatc gctattggac cttcaggagc cgctttgatc ccattgatct ctgacggtaa
     3361 atggttaggc tacatccttg gattattggc agcttatgct ggtggcttcg ttgctacatt
     3421 cttcttcggt attcctaaag accgtttaga aaaagaagaa ttagcgactg aaacagccac
     3481 agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg tgactatcct
     3541 ctcatcagtt gctgacggaa cagttgtttc tttagataaa gcaagtgatc cagtttttgc
     3601 acaaaagatg atgggtgaag gctactttgt agaaccagaa aacggacata tctattctcc
     3661 agttactggg gtagtcagct ctgtcttccc aacgaaacac gctattggga tcacaacgcc
     3721 aaatggttta gaaatcttgc ttcatatggg aatcaacaca gttgacttag gcggtacgcc
     3781 atttgatgtg aaagtaacag aagggcagca agtgacacct gatacgcttg tagcagaagc
     3841 tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa
     3901 tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc
     3961 tgtgatggat gtcaaagctt aatatgatag aaacaataga aacgaaagaa gaaactgaaa
     4021 tctatttttc agcttcttct tttttattat ttattgtttt ctgctaaaat ctgataaatt
     4081 cgtgtcacat cagatggtgt tccccgtaat tcataatctg ccaagaaagg aaagccaaat
     4141 gtttgtgcat attgtttggc tgttagacaa tactgattat tgaagttttt atttccgcta
     4201 ccgactacac caagacaaag tttatgattg tcttcatgtt ccagatattc acgcatcgtt
     4261 tcggtcaaga tttctgtatc accgttatct aaaccatttc cgccgtctaa ataagtagga
     4321 acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac tattttcatg gatttctttt
     4381 aaagtaactt caggattgac aggatcttct gcatgcattt tttcagcata ttctgctaga
     4441 tgtttggcaa aggcacgcgt atttcctgaa atcgatatat ataaaatgtt catggatcat
     4501 cctccaattc tttcctttta gtatagcatc ttttttaagt tttttggtgg tgtaatttgg
     4561 aaagggacta ggcgctttgc ttgtataatg agaaaaaagc cggtagaatt atatttctat
     4621 taaagttttt attaattaag taaaaataaa aaaggtttgt tatacttttt gataaaagcg
     4681 gttttatttt ggaggatgct tacatggata cagagcaaca gaaaaaagga attggtttaa
     4741 ttccacttgc tgccttagta attggttcag cgattggagg tggcgtattt ggtattatga
     4801 cggacatctc cggatcggct ggcggcccag ctattttcag ttgggtactc gtaggaacag
     4861 caatgttgat gctctccttg tcgatcaata atatcaataa aaaacgtcca gatttaaaag
     4921 gcgggatctt tagttacgca gaagaaggtt ttggccgttt cgctggtttt atcagcggtt
     4981 ggggttactg gctcacatgc tggttaggca acgtagcttt tgcaacattg ttgatgagcg
     5041 cattaggata tttcttccca gtatttgaag gtgggcaaaa tctggcatcg atcgtgatga
     5101 gtacagtctt tttatgggga tatgcatggc tagtgaatcg gggagtcgaa aatgctagtt
     5161 tcgtcaatgc tatcgtcaca ttctgtaaat tagtgccatt atttttattt attgtagtag
     5221 ctatcagcac gttttcgatc caacaattca ttgataactt ttctagcgac attatattga
     5281 atgcgaatgg acagccgatc ccgttttcca gtcaagtatt gagttgcatc atggtgatgc
     5341 tttgggtatt tgttggtatt gaaggagcct ctgtcgtagg ttcacgagca aaaaaacaat
     5401 cagatgttgg gaaagcaacg attctaggga tattcggctt gattattatt tacgtcttgg
     5461 tttccatgct tccatatggg acaatgacga tgaatcagct gcaaacgatc aaggcgcagc
     5521 ctgctatggg ctatgtgttt gaaatgatgg ttggtaaatg gggagcagtc gtgatcaacg
     5581 gcggtttgat catttcttta gttggtgttt ggctctcatg gacgatctta ccgattgaaa
     5641 caatgggaaa catggctgaa gacggacttt tgcctaaagc atggggaaaa ctcaacaaaa
     5701 atggggcacc aacttatgct atcgtattga cgacgttatg tacgaatgtc tttttacttt
     5761 cattattatt cactgacagt gcttataact ttgcttacac acttggtaca gcagctattt
     5821 tcttcacgtg gcttttctta ggattgtacc aaatgaaact ttcttatcag cggaaagaat
     5881 gggtccaatt tttagtaggg cttttagcaa gtgcctttca agtatgggct atgctagtcg
     5941 tagcctttaa tgaagtaatg cttgttcttg ttcttttctt gccaggcatt tatttttacc
     6001 tcaaagcacg taaggagcaa aacgagcctg tacaacatat ccacttagat aagattttac
     6061 ttataggtgt gacgatggtc ggagtgattg caatggtttt atttgccact gggatgcttc
     6121 acgtatagaa gtataaaaga agtataaaga gaggttgtga gcctgtcgtt tattcaaaaa
     6181 catactagga aaggagctgt gacacaaatg ttgtcacagc tcctttttca gaataaacgg
     6241 tgtttaagag acagccaatc ggcaataagg ctgaacgtct ctttcacagc ctcttttttg
     6301 atgagtcatc atacattttt tcgcttaaat acgagatatc ctaatgctaa gaacaaaacg
     6361 atgtaagcta aattaccgaa tagcatttga ttagtagaaa gatgagtcaa ttgcttcatg
     6421 atctcttcgt ttccaatttg gttttgcaga ttcaacatac tgatcggatt ccatttgatc
     6481 cactcccatt tttggatgag tgcaataagg ataccagata tgattgacga agcaaaataa
     6541 aagacgattc ctgctgagat ggatgcaccg gaactattga taaagcaagc aatcatcaac
     6601 actaaactta aaatcagcca taaaccgata taactaccca aagtataagt gaacaagctt
     6661 tggatcagtg tttgaccagt agaaaccttc tccgtgaagg aaatcgaagg gaaaagaatc
     6721 agtttcatca acacagtgac aataatagct aaaaggtata aataaacaga ataaataact
     6781 aatgtgatcc atttgcttac taatatttct cctcgagaat actttcgata aaggagattt
     6841 ttcaatgtac catattgtgc ttccatcgaa atgatcgtgc ttgcagctgc aatcatgata
     6901 aagacaatcc aagatgtggc actaaaaagc tgagcagtca tttcaactgg atcaatgatc
     6961 gtcgtgtatt ttttggctag aagtgctgtt cctatgagca aaacaactaa tagaacagat
     7021 aaaacagcag ttgacttttt cttgatcaat ttaaataatt cttgttttac aagcaatgtc
     7081 atttttcttc ttcccccttt actgaatcca gaatttccaa tagagaagat tccaaatcat
     7141 tcgtcagatg ctggacgtct gtcacatgga tattttgtgc agacaatagc tggatcacgg
     7201 ttcctaataa ttcttcgttt tcttttaaaa gcttgatttt atcaggatct tccaaagtat
     7261 atccagcttc tgtcaagatc gttcgagcag ctagatcatt tgttgtagag ataacgatga
     7321 aatcttgcgc tgtttggttc aattcagcca ttgtacattg tttgatgatc tttccttgat
     7381 tgatcaggat cagctcatcg accatttttt caagctcgct gagtaagtga ctggagatca
     7441 agaaagaggt gccctgagta gccaagtcgg taatgatttt tcgcacatct cttgttgctt
     7501 gtggatccaa accgttcatc ggctcatcta aaataacgat agacggatgg ttcagtaagg
     7561 caagagcaat tcccaatttt tgtttcattc ccaacgagta ggatttcgct tttttgtgaa
     7621 tgtacttttc catcttgagc tgattcacga tagtgtcgat agcagataca tcgttggttt
     7681 cagagaataa cttcagatga tcatagcctg ttaagaatgg atagataccc gggtattcga
     7741 tcaatgctcc tacttgttcc aaccctttat gactagaggg agaaatctca ttttcaccaa
     7801 tacagattgt tccatctgag taatggatca gtcctaaaat agctttcatg atcgttgttt
     7861 ttccagcgcc attaggacct actaatccaa cgattttgcc aggggataag ctaaaactga
     7921 catcgctgag ggcttgtttg tgaccaaagt tcttggataa ttcttggacc gaaagacttt
     7981 tcaaaaatat tcactccttt tctatagata gtacaagcat acacctgatg agtggagaag
     8041 tcgctaggtc attggactta tttcttattt tttcataaaa aaattgaata ttaaataaaa
     8101 aggcttattt tttctgatca gtacttattc caagtgtcta gtggccgcta gtaatcttga
     8161 tgccaatgat actgatgagt agcatcccca caaagaacca agtggaaaga ttcagcttat
     8221 catgaaacaa gaccacacca attatgaccg aaccaactgc gccaatccct gtccagatag
     8281 gataagagag actcatcggc aaacttttga ttgcctttga caaaaagtaa aaactagcaa
     8341 tcatgccaat cactgtgtaa aaagaataat tgattttaga aaaaccatca ctccatttca
     8401 tcatcgttga ccagaatact tccaaaattc ctgctatgat taattccatc catgccatac
     8461 ttacattcct ccttaactat aaaaaaatac acaaaaagag gaaaatcatc ccttaaaaga
     8521 cctaatggaa tgatcatcct ctttatccta cccggtggca gatcgtatat ctttttctgt
     8581 agtatagcac acctatccag atatcggcaa tctcatactt tttagtaaag tgtaccaagc
     8641 actgacggat atatcgttag cggaaaagaa gatgaatgct ttgcaatttc atagaaagaa
     8701 gttgttattt attaatcatt taaaaaaaac agtaactaga ccttatttct atatattatc
     8761 gg
//
LOCUS       DAZKNO010000115         8731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000115 DAZKNO010000000
VERSION     DAZKNO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.117"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG8MY_001983"
     tRNA            2..74
                     /locus_tag="ACG8MY_001983"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG8MY_001984"
     tRNA            78..150
                     /locus_tag="ACG8MY_001984"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG8MY_001985"
     tRNA            155..225
                     /locus_tag="ACG8MY_001985"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG8MY_001986"
     tRNA            238..311
                     /locus_tag="ACG8MY_001986"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG8MY_001987"
     tRNA            323..394
                     /locus_tag="ACG8MY_001987"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG8MY_001988"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG8MY_001988"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0431163.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG8MY_001989"
     CDS             1983..3455
                     /locus_tag="ACG8MY_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0431164.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG8MY_001990"
     CDS             3538..4326
                     /locus_tag="ACG8MY_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0431165.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG8MY_001991"
     CDS             complement(4393..5061)
                     /locus_tag="ACG8MY_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0431166.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG8MY_001992"
     CDS             5176..6888
                     /locus_tag="ACG8MY_001992"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431167.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACG8MY_001993"
     CDS             6891..8663
                     /locus_tag="ACG8MY_001993"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431168.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2622 a         1487 c         1944 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc a
//
LOCUS       DAZKNO010000116         8574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000116 DAZKNO010000000
VERSION     DAZKNO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.118"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..1104)
                     /gene="menA"
                     /locus_tag="ACG8MY_001994"
     CDS             complement(151..1104)
                     /gene="menA"
                     /locus_tag="ACG8MY_001994"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0431169.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1117..2220)
                     /locus_tag="ACG8MY_001995"
     CDS             complement(1117..2220)
                     /locus_tag="ACG8MY_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0431170.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2310..3245)
                     /gene="pplA"
                     /locus_tag="ACG8MY_001996"
     CDS             complement(2310..3245)
                     /gene="pplA"
                     /locus_tag="ACG8MY_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0431171.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3547..5481
                     /locus_tag="ACG8MY_001997"
     CDS             3547..5481
                     /locus_tag="ACG8MY_001997"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0431172.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5938..6354
                     /locus_tag="ACG8MY_001998"
     CDS             5938..6354
                     /locus_tag="ACG8MY_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0431173.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6368..6934
                     /locus_tag="ACG8MY_001999"
     CDS             6368..6934
                     /locus_tag="ACG8MY_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0431174.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6957..7943
                     /locus_tag="ACG8MY_002000"
     CDS             6957..7943
                     /locus_tag="ACG8MY_002000"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0431175.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8098..>8574)
                     /gene="scm"
                     /locus_tag="ACG8MY_002001"
     CDS             complement(8098..>8574)
                     /gene="scm"
                     /locus_tag="ACG8MY_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0431176.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2520 a         1659 c         1738 g         2657 t
ORIGIN      
        1 cgactttttc acaacctcct gataaacggt gttcaaaagc ttgcatctgc ggtaataagc
       61 ccaaccaaac gaaaaatata aggagctatt tttatttggc tgttcttatt acttgatgca
      121 gggcagtttt ttcacaacct cttcatgctg ttaaatggct tgcataatga ttgttagtaa
      181 tagccccaat acataactgg cattgaataa gaccatattt ttgatggaat agctgaaact
      241 gataggctgg ggtaacgttt gcttgaattg atccaaatgc ttcttcacaa ttggcaaggt
      301 caagaaggtg accaagatcg gccattcata gataccgctc aacaacccca ttaaaatcac
      361 gaaatagcaa gcatacatca atagctggaa taagactgtt cccgcaggac gtccgatata
      421 aaaaactagt gtatagcgat gattttcaat atcacggtca agatcccgca aattattagc
      481 caacatgata ttggcaatcg taaacaccaa gggtaaggaa gctaatacga ttgctgctat
      541 agcccaaaga tttcctacca aatcaaattg tccagatgaa aagttgactg tcagatcata
      601 gacatctgaa ccgaatgtat tgacataaac gaccatggca aaaattccaa ggcccatagt
      661 caccccactg aaaatttccc ctaaaggcat tcgagataaa ggaatcggtc cgaatgtata
      721 gaatactcca ataaaacaac agataaatcc catcaaaagt aaaagccaac ccgtattgat
      781 cgttagaata acgccgataa caaaaacaaa tgcaatcata gctaaaatca tgtttcgaat
      841 caatacagga gatagatttc tgcggccaat gacattttct tcatacttat atacttcaga
      901 atgagccttt tcataatcca tgtaattatt gatagcggta gtcgctaaat caaaaactag
      961 cattccaata aaaaataaaa aagtgtttag ccaatgtatc tcatgaaaaa aagcgataga
     1021 gaataatacc cctaccgcaa aaggaaatac actagccacc tttgttcgta tttcaactac
     1081 ttctaaaaag atttttagtg tcatggttcg tactccttat tttagttcag aacggtcacc
     1141 catcgtgtaa tgggagtcct ccgataattt gaaggtgtct ttgattggat cggtcacata
     1201 tactttatcg tcttttgtca cgaagatcgc ttccgtcccg ttattcaatt cgctttcgac
     1261 gtattccagt ccttttttca cacccattga aaagactgct gtagacaacc cgtcaccatc
     1321 aattgattta tctgttatga tcgtgacact tgcgatatca ttgtcgaacg gataacccgt
     1381 ttttggatca aacaaatgat ggtatttttt gccatctact tctaaataac gctcgtaaat
     1441 tccagaggta actaatgttt tgttccgttc tttaatagaa cctaaaacag atccgcgagc
     1501 catgttcgga tcttggatgc cgactgtcca gtcttggttt tcgcctcttg ggctgtggcc
     1561 taagacatag acattaccgc ccaagtcaac gatggcagtg gtcactcctt gttttttcaa
     1621 gactttcact acttcgtccg taatgtaacc tttggcgatt gcacctaaat cgatgatcat
     1681 tcctttttct tttagataaa cagttttttc tttgtcattt agttcgatct tatgataatc
     1741 cactaatttc aatgcctgat cgatttcttc ttgggaaggt tttctggcgt catcaaaacc
     1801 gattcgccat aattgtgtga ttgcaccaat cgccatatcg aatccgcctt gagaatcttg
     1861 gctatattcg taggcccgct tgactaacgt gtagacgtca tcagaaactt ttactggttt
     1921 gatcccagct tgctcattca cttcatcgat ctcagaacct ttttgattga tcgtgatttt
     1981 atcaccaagt tcttttactc gatcgaaggc aggtttcaat gctgattctt tgccttcatc
     2041 atacacacgg atacgaacgt aagtccctaa caagaactgt tcatcagaat atggatcttt
     2101 caatatcttg gtagctgctg aactttcagt ggttgtttta tttgctgagt tgctgcagcc
     2161 agttagcacc ataaaagaga tgcccagtgt gattaaggaa aatagtagtt ttttccgcat
     2221 ctttttcctc tcttcatttt gaaaggtgag acgactgcct aatctggcta tcgtcccacc
     2281 ctccaacttt tattagtatg attatgtgat tatttttctg ttacgatatt atctacttcg
     2341 attgtttgag tgtcgccttt ttcagctgca ttcactaatt gttgtgcgta catgatgaat
     2401 gaatgtgagc tgtgtgtagc acctgtaaca acttctacat tagcaggtga gccatcttct
     2461 tcgcccattg ctttgacgaa ttcgtcgttc agagtaggga tgtaagtttt tggttctgta
     2521 ccgctttttg ctttcatatt ttcattatat tctgtatcat cttgttttga tttaccgtct
     2581 ttgtcgatat aatcaaagtt tgattcagtt actttgccgc cagcaactgt catttcgaat
     2641 tgcacacggt agccgttaga gtagttcttt tcttcaagct tgtaagtgcc gtctttcaaa
     2701 tcagcaccat tgtcgatttc gattgtatct gtgttaccag cttgagcagc ttggatcaat
     2761 tgttgtgcgt agttttggaa gctttcagat gagtgagtag ctccagttac gacttccaca
     2821 ccgcttgcgc tttgtgcttt taagaattgg tcattcaact caggaatgta tttttctggg
     2881 tttgtgccag attttgcttc catgtttttg ttgtattctg catcttttgt ttttgattcg
     2941 ccgttttcat tgacgttatc atattttgat tcagtgattt tgccgtcttt tacaaccatt
     3001 tcaaagactg cacggtaacc atttgaataa tttttttctt ctaatttata tgtgccgtct
     3061 tgcaaatctc cgccagctac aacttttgca gatgattctg ttgtgctttt tgcagtcgaa
     3121 gattgtgctg tagatgattc agaagaagag tttgagcttc cgttgtcatc tgcgccgcaa
     3181 gcgcctaaca ctagagctga aaatgctaat acggcaaatc cagttacaaa acgttttttc
     3241 ttcattttaa taccccacca ttccatttct agtccacaat ttttctttgt gattcattat
     3301 acattctaat ctttttagcc tataagtcaa ctatttttga taaataaccc atgtaatatt
     3361 tcaaacgttt tctttcagca aatttcgtga aaaaacgctt tattttaaaa tttgaaaaaa
     3421 atccaaaagg agttagacaa aagaacagaa catctatata atatatggag attgtgtaat
     3481 aagtatcaaa ctaaaagaat tatcatatat tcattatata gatagtctat aaggagcgga
     3541 tgatcaatgg caaagcaaaa tattgtcgtt gtaggagcag gatatgccgg tgtttcagct
     3601 acaaaatact tggcaaagaa actgaaaaaa gaagatgtag caattactct gatcgatcgt
     3661 cattcgtacc atactatgat gaccgagttg catgaagtag ctggcggacg tgtagaacca
     3721 gaagcaatcc aatacgatct gcagcatttg tttgctcgtc aaaagaatgt acaacttgtc
     3781 acagacactg taatcggtat cgataaagaa cgaaaaatcg tcaagaccaa attaggttcc
     3841 tatccttttg atcagctgat ccttggaatg ggcggagagc cgaatgactt tggtacacct
     3901 ggtgtcaaag cacacggatt caccctttgg tcatttgaag attctgtaag gattcgggaa
     3961 catattgaga agacagtagc aaaagctgca ttggaaccag atgatgaact gcgtaaagcc
     4021 atgctgactt tcgttgtctg tggttctgga tttaccggta ttgaaatgat tggggaactg
     4081 atcgactgga aaaatcgcct agccaaagat tataaattag atccaaacga aatcacactg
     4141 atggtcgtag aggcaatgcc gacgatcttg aatatgttgg atcgaaatga tgctgcaaaa
     4201 gctgaacgct atttgcgtaa gcacaacgtg gaattattat tgaatgcacc aatcgtagaa
     4261 gtggctgctg atcatatcaa attgaaagac ggacgcaaca ttccgactca cacactgatt
     4321 tggacagctg gtgtcaaagg aacttctgac gctgctgact ttggattaga atcagcacgc
     4381 ggacagcggt tgatcgccaa tgaatatatg caggcaaaag gctacgaaga taaaaacatc
     4441 tatatcattg gtgatttagt cttttatgaa gaattcccag gaacaccgac accgcagatc
     4501 gttcaagctg ctgaacaaac aggacacaca gccgcagcga atatcgtggc ttcgatcaaa
     4561 ggcggagaaa agcataaatt taaaggaaac tatcaaggat ttatggtttc tattggtgcc
     4621 aaatgggggg tagctaacct atttgataag atccatttaa gcggattttt agctatcgta
     4681 atgaaacata tcgtgaattt gaaatacttc tttgatattc gttctggtta ttatatgttc
     4741 caatatttaa tgcatgagtt tttccatatc aaagatgatc gcaacgtgac tcgaggacat
     4801 tcttctcgtt acggcaatgt cttatggagt gtgccacttc gtattttcta tggtttagtt
     4861 tggttcgttg aagcttccaa aaaaatcatt gggaatggtg aagtgttcaa accaagtact
     4921 tggtttggtg aaggatcatg gttcactgga aacagtgtgt tcccttggac atgggaatac
     4981 aatgtaacag atgttgccag tggtgcttct gctgctggtg atgcaaccac agctgccagc
     5041 ggagcagggg caagtgccgg agaagcagcg actcaagctg cacattttgg tttgagttat
     5101 gcatacggtg aagaaccaat ggctgtgatt gagaagtcac cagactggtt cacaaagatc
     5161 atgcaagtga tcatgccaaa caatgatgta gcaatgttct tccaaaaatt catggtattc
     5221 tttgaaattg ccttggcatt agcattgatt gctggattat tcacatggct atgcagtgcg
     5281 acaacgattg tattggtgat cacgttctgc ttatcaggca tgttttactg ggtaaacatt
     5341 tggttcattt ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat
     5401 cgctgggtaa ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt
     5461 tcccagtacg gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag
     5521 aaaggggctg agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg
     5581 cccgagataa gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt
     5641 tataaacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt
     5701 gaaaagtaat tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc
     5761 tcttgttata aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa
     5821 acatggaaag ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac
     5881 aacctcttgt ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg
     5941 agccttaaag aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg
     6001 cttttgactg ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca
     6061 acagcagaaa aagaagcggt actgcgtgtg gatggcacag aaatcaaaac attccctttg
     6121 atggctggag aagagagcaa cacatatact tacaaagatg aacacggaga ttataacttg
     6181 atcgagatag atggcgatcg agtacggata aaagaagcag attgcgatga ccagatctgc
     6241 gtacgtcgag gctgggcaac gaaaaatggc gaaaccatcg tctgtttgcc acataaatta
     6301 gtgatcgaag tccgttcaac agatggaggt gacgaagaca gtctgattta ttagaccttc
     6361 acacaccatg agtaaattac aaaaaatgat ttatatttcg ctacttgttg cacaaggagt
     6421 gatcattggt ttattggaaa atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa
     6481 actaggctta gccaatctaa tcacgatcgt tgccattttt accatgaaaa aacaagacag
     6541 cttcttacta ctttggctac gactattcct gactactttg ctaggcggaa cgatttccac
     6601 attcctttat agtatgagtg gtgctttgct tagttatttt ggaatgttat tggtcaagca
     6661 attaggacca aaacgtgtca gtatcatcgg aattagtgcg gccggcggtt tcatgcataa
     6721 tgtcggacag cttttgatgg cttctttcat tgcacaatca tggacagtca tgttgtactt
     6781 gccagtcctt tcagttttag ggattttatc agggattgca atcgggatcg cagcgaatta
     6841 tcttttacga catgtgcaga cacttcaacg attccaaaaa gattatgagc agggaaccaa
     6901 acaaaaatgg agcccaatgt ttaccaaaaa ataatcgtca cacaaggagt ttttttatga
     6961 agctacatcc aatgtgggaa aactatcctg ctttaagtaa agaattgaca gaaacactaa
     7021 aacggatgga agaaactgtc cgcctgaaaa acaaacctgt cgaacaagcc atcaaagaaa
     7081 cgatccatgc cggcggaaag ctgttgcgtc ctgcctatca gctattgttc tcacaatttg
     7141 gaccagagca agatcgagac aaagccatcg ctttggcagc ttctatcgaa atgctccata
     7201 cggccacact gatccatgat gatatcgttg atgaagcgga tctgcgacgt ggtcagccta
     7261 atatccgtag tcagtttggt aatgtcgttt ctgtctatgc aggagactat ttatttgtct
     7321 gctgttttaa actattgtct gattactcca cttccttgaa aagtctccaa ttgaattctc
     7381 gcagtatgga aaaagtattg aatggcgaat tagggcagat ggatgatcgc tataattatg
     7441 aactttctgt aaaagaatat ttggaaaata tttcaggtaa gacagctgaa ctgttccaat
     7501 taagttgttc tgttggcgct tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag
     7561 gagatatcgg actttctatc gggatggcct ttcagattat cgatgatatt ttagattata
     7621 caaaagagag ccaggaaatc ggcaaacctg tactagaaga catgcgtcaa ggggtctatt
     7681 ctttaccgtt gatctactct ttgcaaaaaa acaaaccaaa gctactacct tatttagaga
     7741 agaaagcggc aatgacagaa gaagatgtcg acgccgtacg caaaatcgtt gaacatacag
     7801 gaggcgtgga agaagctagg aagttagcgg cctcttacac agaaaaggct ttgaaggaaa
     7861 taaagaaatt acctgccacc tctcttcgaa caaaagagaa tctcttttcc cttacccaac
     7921 tgattttgga tcgcaaagac tgaataggga aaggttcgat acgtaaataa atttctcaga
     7981 aggaaatcta cgtgtttttt gacaaattga attgtagttc ttgtatttat gataaaaagc
     8041 aaaaataaag agcaacgtcc gttagcgacg ttgctctttt ttatcttgaa tattttttta
     8101 ccttttcgat ttcttataac tcataaaagt gattcttaaa ccgattacaa gtaatataac
     8161 gcctgcgagt gttatccaat tattcgattg acttccagtt ttaggaagtg ctctttttga
     8221 tttgtttcca ttgtcattgc ttgttttatt agttaagtta aagttcgtcc tgttatccga
     8281 acttgttttt gagaaggaga cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc
     8341 tgtactatcg cttgaagttg atgaatctga gctatcactt gtggtagagg atgtatctgt
     8401 actgtcactt gaagctgacg tgtccgtgct atcacttgta gtggtagatg aatctgagct
     8461 atcacttgtg gtagaggtag tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc
     8521 acttgttgtg gtagatgtat ccgtactgtc gcttgaagct gacgtgtccg tgct
//
LOCUS       DAZKNO010000117         8502 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000117 DAZKNO010000000
VERSION     DAZKNO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.119"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..267)
                     /gene="acpP"
                     /locus_tag="ACG8MY_002002"
     CDS             complement(25..267)
                     /gene="acpP"
                     /locus_tag="ACG8MY_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0431177.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(389..1390)
                     /gene="plsX"
                     /locus_tag="ACG8MY_002003"
     CDS             complement(389..1390)
                     /gene="plsX"
                     /locus_tag="ACG8MY_002003"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0431178.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1439..3475)
                     /gene="recG"
                     /locus_tag="ACG8MY_002004"
     CDS             complement(1439..3475)
                     /gene="recG"
                     /locus_tag="ACG8MY_002004"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0431179.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3592..5274)
                     /locus_tag="ACG8MY_002005"
     CDS             complement(3592..5274)
                     /locus_tag="ACG8MY_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0431180.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5326..5688)
                     /locus_tag="ACG8MY_002006"
     CDS             complement(5326..5688)
                     /locus_tag="ACG8MY_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0431181.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5958..6146
                     /gene="rpmB"
                     /locus_tag="ACG8MY_002007"
     CDS             5958..6146
                     /gene="rpmB"
                     /locus_tag="ACG8MY_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0431182.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6285..7244
                     /locus_tag="ACG8MY_002008"
     CDS             6285..7244
                     /locus_tag="ACG8MY_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0431183.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            7284..8414
                     /locus_tag="ACG8MY_002009"
     CDS             7284..8414
                     /locus_tag="ACG8MY_002009"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0431184.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKE
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
BASE COUNT         2458 a         1807 c         1517 g         2720 t
ORIGIN      
        1 atccttcttt gcttttattt gaaattattt aagatggctg ctgatataat ctactgcgcc
       61 accgactgtt tcaatttgtt cagcgtcttc atctgaaatt tccgtaccga attcatcttc
      121 aagctctaaa acgaattcca taatactaat tgaatcagct tttaaatcat ctttgatgtt
      181 caattcatcc gtcactttat cggtatctat ctcaaaatga tttgaaataa ttttcgctac
      241 tttgttaaat acttcttcgc gagtcaactt tttcacctcc aagaatcata cacttgtcaa
      301 ttacctacac catggctaat tttactggca tttttcagga atgtccacct ttttttcaat
      361 ggaacggatc gcttcagata tccaaatact attcttcgct tttttcaaat tgttcgacta
      421 attgtccaac tacatctgtc tcaagcattg tgtggatttg gcggatcgta tagcgaacag
      481 catcggggcc tgttgctcca tgagttttaa tgactggtgc tttcaagcca aacaaaacgg
      541 caccgccatg tttggaataa tccatttctg ctttcaaaga acgtaagccg tcttttaaaa
      601 gcatagcgcc catttttcct ttgatccctt catttagaat agcagatttt aatagattca
      661 tcatattcat tgctgttcct tcaatagatt tcaaaacagc gttgcctgta aatccatctg
      721 tgaccactac atcagccaca ccattgagta gctcacgagc ttctacgttt ccaataaaat
      781 tcaagcttgt ttcattctga aggagttcat aggctttttt cgtcacttca ctaccttttg
      841 ttgcttctgt tccgttgttt aagagtgcga cacgaggacg tccgatacct cggactttac
      901 gtgcataaaa agaacctaaa ataccgtatt gcaacaaatg ttctggtttg ttttctgcgt
      961 tagcgccaag atccagcata tcgaaaccgg caccttcttt ccctactact ggcaaggtag
     1021 acatcaagcc agggcgttcg atatttttga tacgtcctac gatgaacaac cctgctgcta
     1081 gtagcgcgcc tgtattccct gctgaaaata cagcatctgc ttcaccgttt ttcactgctt
     1141 gtgcagcaag gaccattgat gcagtctttt ttcgtcgaat tgcttttact ggctcatcat
     1201 cactattgat tttttcgtct gtatggataa tcgtgatgtt tttttcattt gttaagtatt
     1261 tcttgatttc tgcttctttt ccgtaaagta agaattctac ttctggaaaa tcttgctgcg
     1321 ctaaagccac gccttctaca attgcttgag gtgcgtgatc tccgcccatt gcatctacgg
     1381 caattttcat tgtttccact cctttttccg tcatcagtat aaagtatagc acagtctgct
     1441 aatcgaaata acgattatcc tgattttcta tttggaggct ttgcgctaaa ggctgatact
     1501 caggaagcat ctgccaatcc tttatttgcc aaagggctgc agcctcttct ctggctacct
     1561 ccaaaacatt tccatccgtc accaagtcag caatcgcaaa ttgaggcaga ccagattgac
     1621 ggaagccaaa tacttcacca ggaccacgta attccagatc tttttcgctt agtacaaaac
     1681 cattggtggt ctctgtcatg attttcatcc gttccacacc tagttcattt ttaggattag
     1741 caactaagat acagtaggaa gcatcacttc cgcgtccaac acgtccacga agttggtgca
     1801 gctgtgccaa tccaaatcga tccgcatcca tgatcagcat gacggtcgcg ttcggcacgt
     1861 ttacccctac ttcaattaca gtagtcgaaa ctaatatctg catctggttg tctttgaagg
     1921 cttccatgat tacttctttt tcctgattct tcattttacc atgtaacaag ccaacttcat
     1981 attttggtgc aaatagttca cgaagttttt catagatttc cactgcattt tttacatcta
     2041 atgcttctga ttcttcaatc agcggacaga tgacatacat ttgatgtcct cttgccagtt
     2101 cttttttcgt ccaatccagt acactgtcca attgaggtgt tcttacccag cgtgtttcga
     2161 tcgggatacg tccagctgga agttcatcaa tgatagatac atccatctca ccatatgctg
     2221 taatagctaa tgttctagga atcggagtcg ctgtcataaa taagacatct ggtcgccagc
     2281 ctttttcacg aagtactttc cgctggttca cgccaaaacg gtgctgctca tccgtaataa
     2341 ctagacctaa attctgaaat tcgactccat cttggatcaa tgcatgtgtg ccgatcacga
     2401 cgttaatttc cccttgctct agtttttcaa ggagctcacg tcgttctttt gctttcgttg
     2461 accccgtaat caatgcgatg gttacttcat tgggatcaaa cagctgttgc aagctttcca
     2521 tatgttgttg tgccaagatt tctgtaggca ccatcaacgc accttggaaa cctgctgtaa
     2581 cggtagcgta taacgcaatc gcggcaacca ctgtttttcc gctacctaca tctccctgca
     2641 aaagtcgctg catatgctgc ggactgcgaa gatcccgaca gatttcattc gttacacgtt
     2701 tttgtgcatt tgtcagttca aaaggtagct tttgagtaaa ttgtttcaat ttttggacat
     2761 cataaaggat ctccaaacca ttcgtttctg ctttttctgc ttttttcaat ccttgaagac
     2821 gcatttgaaa taaaaagaac tcttcaaaaa cgacacgtcg tttggcttga tggttttctt
     2881 cgtggtcttt tggaaaatgc atacttctta cagcagaagc tcggtcaagc agtcgatatt
     2941 tctccacaag gttgtttggc agattttctt caatcaatgt gccgtattct tgaaaccctt
     3001 ttttgattag atcaactaat gtcgtttgac gaattgattt gttgacgtga tatatcggcg
     3061 aaaagtcatc cattgtttga gaacctaaaa tcttcatccc gttcaaggct ttacgctttg
     3121 catcccattt accgtatacg gcgatttcct ccgataaaat gactttttct tttagataag
     3181 gttgattaaa aaaagaaacg ttgaacacat catgttcttg catcatacga aattgcaatc
     3241 ggcttttttt ataaccaaat cgattcatca caggaggcga gactacgatt cctttcaaag
     3301 taaccttttc ttgatcctgg atctcgttta gatttctttc ctgaatatct tcgtagcgaa
     3361 aaggataata agtcagtaag tcttcaatcg tttggatgcc caagctggca agactagctg
     3421 ctcttttttc tccgactcct gccagcatag tgattggatc atctaaagaa cgcataaatt
     3481 tttcctccct cctttaacaa aaaaaggact tggtcagcga tcatacctga tcactaaccg
     3541 aaggcctttt atgaatgaat actgtgttag aaagatattc tgacacagaa attattcagc
     3601 agaaaataga taaggataga caggctgatc tccttggtgg atctcgattt ccaaatcagg
     3661 atgtttttct gttagtttct cagcaaattc atttgcttgt tcaactgttc cttcatcacc
     3721 tgccaagatc gttacgatct ctgtgtcctc attgatcatt tgttctagtg tacttaacgc
     3781 tgttgctgtg atatctgggt tagagatcag gatttttcca tcgatcatgc ccaaaaagtc
     3841 tccttctttg atctctacac cgtcaattga ggtatctctg atcgcgtgag tgacttgacc
     3901 gctcacaacg ctagacaaca tttctgtcat cgtttctttg ttttcttcta aagaagcttg
     3961 atcattgaat gaaagcatag ctgtcaatcc ttgggaaatc gttcttgaag gaacaactgc
     4021 tactgggata tctgctactt ctgctgcttg atcagctgcc atgaagatgt tcttattgtt
     4081 tggcaagaca atcactttat ctgcgtttac ttcttgaatc gctttaacaa tgtcttccgt
     4141 acttggattc attgtttgtc caccactgat aacataatta gcgcctaaac tttcaaacag
     4201 ctcttggacg cctttaccag cagcgatcgc tacgatacca tacgggatac gtggtatttc
     4261 ttttttcgca aactcgttca cttcttcatc gtgttccaaa atcgtctcat gctgcaagcg
     4321 catgttgtcg actttgattt tgactaatga accgaatttt tgtccataat tcaagacttc
     4381 tcctgggttt tctgtatgca cgtggacttt gatgatctcg tcatcattga ccacaagtaa
     4441 agaatctcct ataccatcta aatagttacg gaattcttcg taatcgaatg tactatcgac
     4501 tgtaggtcct tctccgattt ttaccatgat ttctgtacag taaccaaaat ggatatcttc
     4561 tgtcgccatt tgaccttgga cactgcgatg atgttctgca ttgaccatat catccatttc
     4621 tgctggagaa ggttcatatg tttcatccgc ttggaattct ccattcaatg cacttaaaaa
     4681 gccctcataa acaaacagca gtccttgccc tccactgtct acgacaccga cttcttttag
     4741 gactggaagc aagtctggag ttttagctaa agcacgcttt gctcctttta caacagctgt
     4801 cattacttca acgacatcat cagtactttt tgctttcttt tctccagctt tcgctgcttc
     4861 tctagctact gtcaaaatcg ttccttctac tggtttcatt actgctttat acgcagtttc
     4921 aactccatga gtgaaggctg cagcaagatc agaagcattt aatgagacaa catctggaat
     4981 ttgcttagaa aatccacgaa acaattgaga caaaataacg ccggaatttc cgcgcgcacc
     5041 catcagcaat ccttttgaaa gcattgctgc taattctcct actttttctg aggttgaatc
     5101 tactactgcc ttagctccac tagtcataga tagattcata tttgttcccg tatcgccatc
     5161 tggcacaggg aatacattta atgaattgac gtattctgca tttacttgca gacgattcgc
     5221 acctgcctgg accatttcct ggaactggct tgcacttatt tctgttacac tcacctagat
     5281 aatcctcctt cagatcgact gctgagttac cagccagtct cctgcctagt ctggcagtac
     5341 acgaacacct tgtacaaaga cattaactga attggcagtt acgcctaata atgcctctag
     5401 attgtatttc actttttctt ggacgttgcg ggaaacttca gagattttcg taccatagct
     5461 gataatcgta taaacatcta cagcaatacc attatcttct tggcggacaa ctactcctct
     5521 tgagtaattt tctttacgca aaatctcagt aatattatct ttaatttgat ttttgctggc
     5581 cattcctaca acaccgaaca catctgtcgc ggcgccacca acaactgttg caattacatc
     5641 attggtaatt tcaatggttc cagctgacgt ttgaattttt acagccatga atgagcctcc
     5701 ttaacgggta aaaaactccc atttttcttc tatcatattt tatcatagcg caacttgaaa
     5761 taaaaggcag ttacccaaag tactcaaaaa ccttgcaaaa tttcaggtat aaccttgttt
     5821 aatttacaat tattcttctc aattgtcaat tgtatctatg aaaatgtact tgcaaattcc
     5881 cattctatat gatatgttag tctggtatga gctgaattat tctattgctc caaatcaagg
     5941 caaaggagga aattaccatg gcaaaagttt gttactttac aggtcgcaag acaaaaagtg
     6001 gaaacaaccg ttcacacgct atgaactcaa caaaacgtac tgttaaacct aacttacaaa
     6061 aagttcgcgt tatggtcgac ggaaaaccta aaaaagtttg ggtgtcaact cgtgctttga
     6121 aatcaggaaa agttgaacgc gtttaattga atgatacttg gaccgttcag atagtgaacg
     6181 gttttttatt tcgccgattg taaaattaag ctagacaact aaaaaagtca tttgtgctac
     6241 actcaaaata gttccgacca aagaattata aggagtgagt acaaatgacc tatacccatc
     6301 ttacaacgga tgaacttgta ataatagagt cttatttcaa aatgaatcaa tctgttgcta
     6361 aaactgccca ttgcttgaat cgttcaagac aaacgatcca taaagtatat ctgttcttca
     6421 agcaaggaaa atcagcctta gaatattatc aacagtataa gaaaaacaaa tcaaactgtg
     6481 gtagacgtcc gcttgtttta cccgaggaac aatcagaata tattcaaaga aaggttgttc
     6541 aaggatggac acccgatgtg attgttggtc gtgcagcgtt tcctattagt tgttctgctc
     6601 gtaccattta tcgtatgttc aaaaaggggc tatttgattc ttctgactta ccgatgaaag
     6661 gcaagcgtaa accgaatgga catcaagaaa gacgtggaaa acaaactttc cgccgctcta
     6721 ttcatgaacg tgaaaaggat tatagccaat tctcaaatga gtttggtcac cttgaaggtg
     6781 acactatcgt aggtctgaaa cataaaagtg ctgtaattac cttagttgaa cgattatcaa
     6841 aagttatcat cacattgaaa ccgtgtggta gacaagcgat tgatattgaa aaaaaattaa
     6901 atcaatggtt tgaatctgta ccgaaaaacc tattcaaatc catcactttt gattgtggaa
     6961 aggaattttc aaattggaaa cagatcagta atgtcaatga tattgccatt tatttcgctg
     7021 atccaggaac gccgtctcaa agaggcctaa acgagaattc taacggattg ttacgtagag
     7081 atggtttatt gaaatctatg gatttcaatt cagtagatga attttttatt caatctgtcg
     7141 catctaaacg aaataatatt cctagaaaat cactggatta tcgaacacct ttggaagtat
     7201 ttttgagtta cgtaagtatt gatgatctgt ctaacttaat ttgacaatta agattacaaa
     7261 aaacaaagaa aagagtgagt gaaatgagca aaaatagacc atttacagta gatcatgtcg
     7321 gaagtttctt acgcccgcaa tcgatcaaag aagcaagaaa aaaagttcaa gaaggcttac
     7381 tttcaaaaga agaacttcgt gcaatcgaag accaggaaat aaaaaaatta gtggaagacc
     7441 aaaaagcagt cggcatcaaa ggaattacag atggtgaatt tcggcgtgga ttttggcatc
     7501 ttgatttttt agaagaacta aatggtatcg aaggctatgt tccagaaact ggatataatc
     7561 aaacattcca cggaaaagca gcaccagctt ataatattcg ggtagtagat aaattatctt
     7621 tcaatccaaa tcacccattt ttaaaagatt ttgcttttct gaaagaagta gtggaaacag
     7681 atgatacttt tgttgcaaaa gcaacgatcc ctagtcccac gatgatttta cgacaagaga
     7741 tcctatctaa tgatggttct tcaaaaatca aggagattta tcctgatcta tctgattttt
     7801 atgaggactt gtcctctact tacaaagcag cgattcatgc tttttatgaa aaaggttgcc
     7861 gttacctgca attcgatgat acaaattggg cgtttctagc tgatcaaact aaaagagaag
     7921 cacttcttca aaaagaaatt gatccgaaag aaatggccca tatttgtaca gagattatca
     7981 ataacgcact ggaagacaaa ccagaagatt tgacgatcac aacacatatc tgccgcggga
     8041 atcacgcttc ttcttggctg ttttccggtg gatatgaacc cattgcagaa gagttgtttt
     8101 caacaaatta tgatggtttc tttttggaat acgattctga ccgagcagga gattttactc
     8161 ctttaagaca ttggagtaac aaagaaagta aagttgtttt aggattagtt acttctaagt
     8221 ttccagattt ggaagatacg gagaaaataa aagaacgaat aaaagaagca caacaatttg
     8281 taccattgga aaatctaagt atcagtcctc aatgtgggtt tgcttcaaca gaagaaggaa
     8341 accatttaac agaagaagaa caatggaaga aaatccaatt attgcagcaa gtcgcaaaag
     8401 aagtttggct ataaaaatgt gaggcttggc aaaaaaaagc gtcccaaaaa gtcggctttt
     8461 catggagtgg agaaatccac tcttttttat ttacctttta ta
//
LOCUS       DAZKNO010000118         8485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000118 DAZKNO010000000
VERSION     DAZKNO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.120"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            111..2720
                     /gene="clpB"
                     /locus_tag="ACG8MY_002010"
     CDS             111..2720
                     /gene="clpB"
                     /locus_tag="ACG8MY_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0431185.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2871..3557)
                     /locus_tag="ACG8MY_002011"
     CDS             complement(2871..3557)
                     /locus_tag="ACG8MY_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431186.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3535..4029)
                     /locus_tag="ACG8MY_002012"
     CDS             complement(3535..4029)
                     /locus_tag="ACG8MY_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0431187.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4049..5269)
                     /locus_tag="ACG8MY_002013"
     CDS             complement(4049..5269)
                     /locus_tag="ACG8MY_002013"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0431188.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5416..7251
                     /gene="lepA"
                     /locus_tag="ACG8MY_002014"
     CDS             5416..7251
                     /gene="lepA"
                     /locus_tag="ACG8MY_002014"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0431189.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7345..8472)
                     /locus_tag="ACG8MY_002015"
     CDS             complement(7345..8472)
                     /locus_tag="ACG8MY_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0431190.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2729 a         1624 c         1703 g         2429 t
ORIGIN      
        1 tcaaaaaact atgtgtatat tttgaccaaa attgaccaaa gcgatataat agtttttgta
       61 aagatcaaag aaataagtga tcttaccttt aattaggagg gtatacctgt atgaatattg
      121 agaaaatgac tacgacgctt caagaagcga ttgctgaagc tcaacagatt gcggtcactc
      181 gaaaacatca agatatcgac attgcccatg tatggaaaat ctttttacaa tctaatcatt
      241 ttggtcgaaa tttctatacg gatgccggat tggatgtcga atcttttgaa catgaaattg
      301 atcgtctact ggatgagtat ccagttgttt ctggtggaaa cgtccaatat ggacaaaacc
      361 tcagccagaa tttctttcga ttattgaatg aagcagacca aatacgtgaa agcttcggcg
      421 acgagtttct ttcaacggaa gtcgttattt tagcgttgat gaaattaaaa aattatccgt
      481 taactgttta tttgaataaa aacggattat ctgaaaaaga attgcgtaaa aacatcgaag
      541 agatgagagg aggagaaaga gtgacctcac aaaaccaaga agaacaatac aaagcattag
      601 aaaaatacgg cgttgattta gtacagcaag tcaaaagcgg aaaaatggat ccaatcatcg
      661 gtcgtgatga agaaatccgt gatgtcatcc gtattctttc acgaaaaaca aaaaataatc
      721 ctgttctgat aggagaaccc ggtgtaggga aaacggcaat cgttgaaggg cttgctcaac
      781 gtatcgtacg caaagatgtg cctgaaaatt taaaagataa aacgattttt tcattagata
      841 tgggtgcatt gatcgctgga gcaaaattcc gtggtgaatt tgaagaaaga ctaaaagcag
      901 tcttgaaaga agtcaaaaaa agtgatggac gtatcctctt gttcatcgat gaaatccaca
      961 atatcgtagg ggcaggaaaa acagaaggaa gtatggatgc cggaaatctg ttgaaaccaa
     1021 tgcttgctcg cggtgaatta catttgatcg gtgccacaac tttagatgag taccgtcaat
     1081 atatggaaaa agacaaagcg ttggaacgtc gctttcaaaa ggtattagtc aaagaaccaa
     1141 cagtggaaga tacgatcagt attctgcgtg gattaaaaga acgttttgag atccaccatg
     1201 gtgttaacat ccatgacaac gcattagtag cagcagctac tttatctgac cgttatatta
     1261 ctgatcggtt cttaccggac aaagcaatcg atttgatcga tgaagccagt gcaacgattc
     1321 gggtagaaat gaactctatg ccaacggaat tagatcaagt tactagacgt ttgatgcagt
     1381 tggaaatcga agaagctgca ctgaaaaaag aatcagatga tgcaagtaaa aaacgcttga
     1441 aaaatttgca agaagaatta gctgaacttc gtgaagaagc aaatgcgatg aagatgcagt
     1501 gggaaacaga aaaagaagaa gtcaattctg tttcagcaaa acgtgctgaa atcgataaag
     1561 caaaacacga attagaagat gcagaaaata actatgattt agaacgcgct gctgtattgc
     1621 gtcatggtac gatacctcaa ttagaaaaag aattgaagga attagaagct aaagcaaaag
     1681 acagcgagat taagatggtt caagagtccg ttaccgaaaa tgagattgct caagtcgttg
     1741 gcagattaac gggtatcccc gtaacgaaac ttgtagaagg tgaacgagaa aaactaatta
     1801 aattgaatga aacacttcat aaacgagtga ttggtcaaga tgaagcagtt gatgcggtca
     1861 gtgacgcggt aattcgttca cgggctggat tgcaagaccc aaatcgtcct ctaggttctt
     1921 tccttttcct aggaccaact ggtgtcggaa aaaccgaatt agcgaaagcg ttagctgaga
     1981 acttgtttga ctcagaagat catatggtac ggatcgatat gagtgaatat atggaaaaac
     2041 atgctgtgtc tcgtctagtc ggagctcctc caggctatgt aggttacgaa gaaggtggtc
     2101 aattaacgga agctgtcaga cgaaatcctt atacaatcgt cttgttggat gaaatcgaaa
     2161 aagcgcatcc cgatgtcttc aatatcttgt tacaagtcct agatgacgga cgcttgactg
     2221 attcaaaagg gcgagtagta gatttcaaaa atacggttct gatcatgacc agcaatatcg
     2281 gttcacagtt actgctggaa ggcgtgactg ctgatggaac gattccagaa gcagtggctg
     2341 aacaagtcaa cacgctatta cgtggaaact tcaaaccaga attcttgaac cgaatcgacg
     2401 acacgatttt atttacacca ttgagcctag acaacgtgaa aggaatcgtt gacaaaatgg
     2461 tggcgcaatt ggcacaacgt ctggaacatc aagaaatcct gctgacaatc agtgatgaag
     2521 ccaaaacgtg gattgctgaa aatgcttatg aaccagcata tggtgcaaga ccattgaaac
     2581 gatttatcac taaggaagta gaaacaccac tagctaaaga aatcgttgca ggacatgtta
     2641 tgcctaaatc aaaagtaacg attactttac ttgatggaca acttcatttc aaaacagaag
     2701 agctagaaga aatcgtctaa tcttccattg agactataaa agaaaaaagg ggtctgacac
     2761 aactagtgtc agacctcctt ttttcaaata aacagtgttc cagaagtagt ttcttcgaaa
     2821 aataagccga aatttcttga agctgcccgc ttctgtcaca acctctttta ttaggtattt
     2881 ttcacttttt ctaatttata actttcaaag taacgctcat cttttttact ttgagaccat
     2941 gcaaacaatc ccattaaggc agcacctatc gtatttgtca gcaagtcgcc catcgtatcc
     3001 atcaatgctg ctcgtccgac aaacagtgtg ccatcagaag cagcaaaacg ttgaagattc
     3061 atgcctaaga actggtcgca taagaattcc cagaactccc aaaacacgcc gcaaagtcca
     3121 gcaaacgcaa agccgaataa gaggaacaac caaggactgg tttttgatat ctcagcatct
     3181 ttcatcaagt aaccaatcaa tccatagcca agagctgtca ggaccattgg actcaccgca
     3241 tgtaagattt tgtcccaaaa agagatgata ctgatcaaat gcatccctgt gcctaaaaag
     3301 acagagatga acaagaaaaa ccagtaaaac aaaacgattg ctgctggtat tttaagtttg
     3361 aaaactttca aaatgacttg tggcatgaaa atcaatgcaa tccctaataa agattccacg
     3421 ataaatgtga ttccttgaga agtcgaatat ttttgtgtga taaattcata aaaattatag
     3481 cagacaacga atacaccaaa agcaagtaaa gctatttgca cctttttgat ttctttaatc
     3541 actgaaagtc cctccatcta aaaatctttt tacttctgaa agcaactcaa tttctgaaga
     3601 aaccactttt ccgttcaatt tgatcaaacc tattgtatat aagttcaggt agtgaaattg
     3661 attttcagct gtttcttgta gtgcagcgat ttttttcggg tgggttccgc cttgctgtcg
     3721 agtatcagta tataagccga tcactgggat gcctttcgca taagccacac cgatttccga
     3781 tgctaccccg gcatcgatcg tcagcccatc tagtaaagca atcattaaat cactagccaa
     3841 cactttttct gtgtcagcaa gagcaatcat tcgactgtct gcataagcag ttttgtcatt
     3901 gattgcttca ttttcttgag gaagatagat cgaaagctca gaagagagct ctctgatttc
     3961 tttaactaac tgttggttat ataacaaatc acttttagca aacattggtg cggcaaaata
     4021 aattttcatc taaacatcat cctttttatc aaaaataatc gctaaagtaa gcttccatgt
     4081 ttggaatgat acgctctagt gacttcagtg tttcttcatc tgtttctaac cgttcgattc
     4141 ttgccaaata agaagcacgg cgataattca tcatcaagca agtcaatgtt tggatatcca
     4201 gtcgaacgga ttttcctata ggttcattta agacacggac ttgtccctgt tcatcccatg
     4261 ttaaagcgaa gacaccatta ttccaaggag cgacaggatc ttcaatgatg aaatgaaaag
     4321 catcagctgt tccaacaaac ggaaaccgtt gaaggaattc ttttacatca acgatccgag
     4381 ccataaaata tggttcgatc tgctctttga tctgactatc ttcaatcaaa aatgctaaag
     4441 gctcattttt ataaatatct ccatgtaccc agtagatcat ggaaaaatgt gcggaaataa
     4501 agttccataa gccatttcga gcttcttgat caagatagaa catttctttt acgtgaaata
     4561 tttcttcagc tacccagtag aacagaacac ctcttggttc atggttgcta tcgtagtaga
     4621 cagcggctgt gcgctcatct tcattttcga aacgccaata ctcttcccaa tgaaaatcac
     4681 tgcggaataa agcaccatgg ttttgttggg caaatttgtc ataaacggaa aatacgtctg
     4741 gatgatcaac tggtaatcgc tcaaccatac cacttactgg gatttgttta ggtagctgtg
     4801 tatctctgat tttaaaggaa agtttatcag acatgatttc ccatccttta cgccgatagt
     4861 aggggatact gtaaggatac aaataagaga tccattgctt attcttgcgc atattatcca
     4921 atgcaacgat aattagatct tgcatcaatc catggttagc atattctgga taagttccaa
     4981 ctccagtcac accacccatt ttataacgtg caccgtgaat attgacttca caaggataga
     5041 tagcgatttg agagatcagt ttattttcgt ggaaccatcc gaacactttc gatacttcca
     5101 aaatcggttt tttggaacgg ataaaagctc gtttattctc aaacccgctt tcctcgatgt
     5161 ctgcttcagt cacttggaac acataggaaa gcaattcatt gaattgatcg atatgttctt
     5221 gagtgaccgg ttttaattca agattttctc taaaatcttt ttgatccatt ttttcatctc
     5281 catttattag cttgataaca gtatagcaaa attctacaaa aaaaacttct atagctgact
     5341 aattaaaaac tctttaaatt actgcttttc gttggtataa tggaaagtgc tgattatatg
     5401 aaaagaggag acgctatgga tataaagaaa atgaaagaac gccaagagca gattcgtaat
     5461 ttttcgatca tcgctcatat cgaccatggg aaatctacat tggccgatcg tattttagaa
     5521 atgacgcaca ctgtgacgtc tcgcgaaatg caagatcagc tgttagactc gatggatttg
     5581 gaacgtgaac gtggaatcac gattaaatta aatgctgtag aactcaatta cacagcgaaa
     5641 aatggagaaa catatatctt tcacttgatt gacaccccag ggcacgtcga cttcacctat
     5701 gaagtctctc gaagccttgc tgcctgtgag ggtgccgttt tagtggtcga tgcagcacaa
     5761 ggaatcgaag cacaaacatt ggccaatgtt tatttagcgt tagacaatga tttggagatc
     5821 ctgccagtca tcaataaaat cgacctaccc gcagcagatc ccgaacgtgt ccgtcaagaa
     5881 atcgaagacg taatcggaat cgatgctagt gaagctgtat tagccagtgc caaatcaggg
     5941 attgggattg aagatattct cgagcagatc gtggaatatg ttcctgcacc aagtggagac
     6001 ttagatgcac cgctaaaagc tttgatcttt gactctattt atgatagtta ccgcggtgtt
     6061 gttctaaatg tccggatcac ggacggtatg gtcaaacccg gagacaaaat caaactgatg
     6121 agtaatggca aaacatttga tgttacagag gtcggggtct tctcgccaaa agcagttgct
     6181 agagactttt tgatggtcgg tgatgtcggt tatatcacag caagtatcaa aactgtccaa
     6241 gatacacgag taggggatac agtaacgtta gcagacaatc ctgccgctga agctttacca
     6301 ggttaccgaa aaatgaatcc gatggtttat tgtggtcttt atccaattga tacttctcgt
     6361 tacaacgatt tgcgggaagc tttagaaaaa ctgcaattga atgatgctgc tttacaattc
     6421 gaaccagaaa cctctcaagc attaggtttt gggttccgct gtggattcct cggacttctt
     6481 catatggatg tcgtccaaga acgcttggaa cgggaattca atctagaatt gatcacgact
     6541 gcaccatccg ttatttacca tgttaataaa acggatggta cgactgctac agtagataac
     6601 ccggcagatt ttcctgaacc tgtaacgatt caggatgtag aagaaccatt cgtcaaagca
     6661 cagatcatgg tcccaaatga ttttgtcggt gcagtaatgg aactttctca acgaaaacgg
     6721 ggagaattca ttacgatgga ttatttagat gattaccgag taaatgtcgt ctataatatt
     6781 ccgctttctg aaattgtctt tgactttttc gacaaattga aatcaagcac gaaaggctat
     6841 gcctcattgg attatgaaat gtctggttat caaaaaagca agttagtgaa gatggacatc
     6901 ttgctgaatg gagaaaaagt cgatgcatta agctttatcg ttcaccgaga ctttgcctat
     6961 gaacgtggaa aagcaatcgt tgaaaaactg aaaaaactaa tcccacgcca acaatttgag
     7021 gtgccaatcc aagcagctat tggacaaaaa atcgttgccc gttcagacat caaagccttg
     7081 cgtaaaaacg tcttggctaa atgttatggt ggagatgttt ctcgtaaacg taaactctta
     7141 gagaaacaaa aagaagggaa gaagcggatg aaacaaatcg gctccgtcga agttccgcaa
     7201 gaagccttca tggccgttct gaaaatggac gaggacgaac cgaagaaata gcaagggttt
     7261 gtttgaaaac agtgtaaaaa ataaaccatg tttacatgga tttgccaccg gtttgccacc
     7321 ggtggttttt ttcggtggca aaaatcaaga attctttcta agattttcca tgatatcgac
     7381 cgtttcattt ctcatcttat ctgttacatg cgagtaagta tccatcgtaa ttgaaattct
     7441 gctgtggccc aatcgttcag agatttcttt catttttgcg ccattttcga gaagaagtgt
     7501 agcatgagta tgtctgagag aatggaagtt aaaagagagg gagagtgcat tcgatatcct
     7561 cctagtattc catttcacta cacttggcgt aactagctca ccgtcttcct tcgtacatac
     7621 tgcatttgaa tcaatgtaga gctttccata cttcattcga ttttctaatt gttgtttctt
     7681 atgttttttc agaattgcta gcaaggtttg tccaataaaa atcgttcgat tggaactgct
     7741 tgtctttggc gtaccatata cccatgcgcc gtcattcttt accatttgtt tctctacagt
     7801 aattgttcca tttgaaaaat cgacatcatc ccacgtcaga ccacaaactt cgccaacgcg
     7861 cattcccgta taaaatccaa tattcaaagg aatatagaaa ggatgtcctt caggagtaat
     7921 ttctagcata tgatcaaagt cctcaagaga aatgattttt agatcttttt tagtcgttgg
     7981 tcgtgcttca tattttggta tttttacata cagcatagga ttttgcttga ttaatcccca
     8041 aggataaacc gccatattca gcgcattctt aaggacagag tgagtaatag tcattgtttt
     8101 cttcgagtaa ccctttttaa attcagcatt gatgaaattt tgtaaaagag caggggagag
     8161 atccgtaagt tttttctttc ctaaataacc gtttatatga tttttgatgg taaatcggta
     8221 gttttcatag gtattgtatt ttagatttag tttaacgtat tcctccatcc aaaaatcaag
     8281 gtattgtttt actcgagtat ccgtacctaa aaagtattgt cctgtttcgt caatatctga
     8341 taaaactttt cgtaaagcag cttcggcctc tggtcgggtg tctccgccaa ctttctccac
     8401 tttttttctt gagccatcat cattgatatc ttcgccgttt gtataattaa gcgagacaaa
     8461 taaaaaagcc atttatgtta cactc
//
LOCUS       DAZKNO010000119         8334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000119 DAZKNO010000000
VERSION     DAZKNO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.121"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(777..2408)
                     /locus_tag="ACG8MY_002016"
     CDS             complement(777..2408)
                     /locus_tag="ACG8MY_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0431191.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="ACG8MY_002017"
     CDS             complement(2422..4095)
                     /locus_tag="ACG8MY_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0431192.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="ACG8MY_002018"
     CDS             complement(4108..4425)
                     /locus_tag="ACG8MY_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0431193.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="ACG8MY_002019"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="ACG8MY_002019"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0431194.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="ACG8MY_002020"
     CDS             6087..6872
                     /locus_tag="ACG8MY_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0431195.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="ACG8MY_002021"
     CDS             6875..7567
                     /locus_tag="ACG8MY_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0431196.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="ACG8MY_002022"
     CDS             complement(7841..8212)
                     /locus_tag="ACG8MY_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0431197.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2559 a         1823 c         1430 g         2522 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caac
//
LOCUS       DAZKNO010000120         8325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000120 DAZKNO010000000
VERSION     DAZKNO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.122"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG8MY_002023"
     CDS             476..1339
                     /locus_tag="ACG8MY_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0431198.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG8MY_002024"
     CDS             complement(1468..3090)
                     /locus_tag="ACG8MY_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0431199.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG8MY_002025"
     CDS             3339..3860
                     /locus_tag="ACG8MY_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0431200.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8MY_002026"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG8MY_002026"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0431201.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG8MY_002027"
     CDS             5941..6759
                     /locus_tag="ACG8MY_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0431202.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG8MY_002028"
     CDS             complement(6862..7059)
                     /locus_tag="ACG8MY_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431203.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG8MY_002029"
     CDS             7228..7806
                     /locus_tag="ACG8MY_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0431204.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG8MY_002030"
     CDS             7901..8038
                     /locus_tag="ACG8MY_002030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431205.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2509 a         1489 c         1642 g         2685 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaa
//
LOCUS       DAZKNO010000121         8203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000121 DAZKNO010000000
VERSION     DAZKNO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.123"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..1371
                     /locus_tag="ACG8MY_002031"
     CDS             172..1371
                     /locus_tag="ACG8MY_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0431206.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(1866..2624)
                     /locus_tag="ACG8MY_002032"
     CDS             complement(1866..2624)
                     /locus_tag="ACG8MY_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0431207.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            2792..3727
                     /gene="pfkB"
                     /locus_tag="ACG8MY_002033"
     CDS             2792..3727
                     /gene="pfkB"
                     /locus_tag="ACG8MY_002033"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0431208.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            3724..5169
                     /locus_tag="ACG8MY_002034"
     CDS             3724..5169
                     /locus_tag="ACG8MY_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0431209.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            5197..5652
                     /locus_tag="ACG8MY_002035"
     CDS             5197..5652
                     /locus_tag="ACG8MY_002035"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0431210.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            5671..6645
                     /gene="lacD"
                     /locus_tag="ACG8MY_002036"
     CDS             5671..6645
                     /gene="lacD"
                     /locus_tag="ACG8MY_002036"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0431211.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(6756..7317)
                     /locus_tag="ACG8MY_002037"
                     /pseudo
     CDS             complement(6756..7317)
                     /locus_tag="ACG8MY_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            7461..8075
                     /locus_tag="ACG8MY_002038"
     CDS             7461..8075
                     /locus_tag="ACG8MY_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0431212.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
BASE COUNT         2539 a         1342 c         1721 g         2601 t
ORIGIN      
        1 cgatgcttct atgatctttt ttccgtcttt attaacagat gttttaattg atcaacgcat
       61 attgtaatct accctaattt ccatagttct tcaccacttt taaaaatagt aaaaccccta
      121 caagtatgct atagtaaatt gctaaagaaa aaccaataag gtgggagatg tgtgcgcgat
      181 caaacaagcg gaaaaatgat tcgtttttat tatacaagtt tgcattttct ttattggatg
      241 atgttttgtt ctgtctatgg gtttgcgaca ttgtttttga tttatttcaa gattgatcca
      301 gctaaaatcg gagggatctt agcattggtc aatattgttt caacggtgct tcagcctttt
      361 gtcagtcaag tgatcatcaa gaaaagaaag atcccactga tcaccgtgct gaagacgatg
      421 atagcaggat taggagtgat tctcttagct ggggtattga ttccgaattt gccgatcatt
      481 acttatatct tgggtgggat tttagtagtt tctatgcaat catttctcaa tgcgctggca
      541 tttgagtatt caaataccgg ctacaggatc aactttggct tttcaagagc tggtggttca
      601 ttttcttatg ctgtaacttc ttttttactg ggacagctgt tagcgattta ttctgccgcg
      661 attttgccaa tcttggttat agttgaagca gtgatatttt tcgttatttt gcatctgctg
      721 ccggatgtaa aaaatcaaga agtttttcct atgacaaaag aacaggatcg ctcagtttct
      781 ttgagaaaaa aatatccttt cctgattttg ttatttttag gtttcagttt tctttttact
      841 ttccacacca tgatcaacag ctttttggca cagatctttg aaaatatcca cggcggcagt
      901 aaagaagttg gcatcgcatt gatgatcgcc gctttatgcg aattaccggg aatgatcttt
      961 tttgaacagc ttgtcaaaaa gaaatccagc aaattttggc tgtctgttgc cagtatttca
     1021 tttgctgtca gaggattcat gatgctgctg gccagttcga tcctgatgat ggaaatgacg
     1081 catctcctac aagccttcag tttcgctttg tttattccag cttcggctta tttgttcaat
     1141 caatttctgg cggatgatga tcgtgtattt ggacaaacga tgatcatcat cgccacaact
     1201 cttggtgggg tgataggaaa tgtcttgggc ggcagtctgc tgcagcattt cggtgtatgg
     1261 gagatgctga ttttcggtac tggttttgcg gtgctaggtg ctttattaac tattttgggc
     1321 attcgaaaaa taccgaagac aggaaacgat agtgattctc ttgttgagta gcataaaaat
     1381 acttagatac tagttttata taaataacat atagtgatta ttggcgtaat tacaaaaaac
     1441 gagaataact taagtaagca tttctaaata attgagaaac ctcatctaaa aagataagaa
     1501 aatatcccta ataaaacttc tgtaacattt tagataactg attgtaatct gaataataaa
     1561 ctataaggag acggttagac ggtctccttt ttttgcgtat tataattcta gaattgttgg
     1621 aaattgataa taattaacaa gaaatattag acaggaactt cgaactgctt atcacattct
     1681 tattattata tatttgcgta agtagtcctg gtagtctcaa tgcactattc tatcttgaaa
     1741 ctaagaagtt atagaaggag caatgataga aagcacgaga atacaaatag cgattaaata
     1801 tttttctaga caaccttagt taattcacac agatatattt taggtacagt cttacagata
     1861 tagagttagt gctgtgtgaa tcctgatgtt cgcagtgcat aggagtcaag taagattcta
     1921 gccagtatag aaagtggaag tctggagcgt acctcataat ctgggaagaa ggagccttct
     1981 gatttaaagc caacaaaagg aatctcgata tgttccatca aacttgaatg acgattagca
     2041 gtaagcaaaa ttgtcccgat ctcagcttta taacagtttt cagctgcggt agtcaattcc
     2101 tttgtttcac cgtttagtga aacaaagatc acgatgtcat ctttggataa ttttctacta
     2161 atgtttttta tgatattggg atcagtatgc atctcacaat atttaccggt aagttgaaat
     2221 ttgaccatca tttcctgagc aatcaattct gaaaaacctc gggcaaaaat catgattctt
     2281 ctagcagcct tgattcgttg aatagcatct tcaatattcc cgctttctac catattgatg
     2341 gtgcggatta cctcttgctc atttttaaga atagcagtct ttattccttc atcaacttta
     2401 tcaagagcgg caaaattgat atttgtattt tctttttctt ttaacgaatg tttaaaagac
     2461 gtaaacccct catatccttt tttcttcata gtacgaacga cggttgctgt tgaaacgttg
     2521 gcatattcac ttagttttac aatggaatag gaggggattt cgagtatgtg actttgaata
     2581 aaatcccaga gatatttctc cgattcactt aattttgtat ccatattttg cctccgatta
     2641 gttaggataa ttctattgta aagcgggagt ttcctttact tcaagtttat ttaatatatt
     2701 ttgtaaaagt tttcaatgaa tttagtcatt tatgaaaaca tttacattcg atcttgatac
     2761 ataatgaata tgtaagaaag gaggagcgta aatgtcagtt tatacatgta ctttaaactt
     2821 ggcaatcgac ctttttatcg aaacagatga aatgaaacca tcccaagtca atcgaacaaa
     2881 cgatgatgat atacaagcta atggaaaagg tgtgaatgtt tcattgatca tgaagcaatt
     2941 aggtatcgat agtacagctt taggatttaa agcaggtttt accgggaatt atattgagga
     3001 ttatttgcat caaaaagata ttagcactga ttttattagc gtaccgggaa tgactagaat
     3061 caacgttttt acacaagtta ataaaaccaa tgaagaattc aaattggtta ataaaggacc
     3121 tgagattcct caggaacaaa tcaaaacttt tctaaatcaa atacaaaagc tgcaagctga
     3181 cgattatttg tgtgtttctg gcagtttgcc gcgaggggtc tcacccgaaa ttcttattga
     3241 tatcagtcgt atttgttttg aaaataaggt ccatctgatt attgattcaa gctatccgga
     3301 aataattgat tgtctgccgt atcagccgtt cttattaaaa ccaaacgagg aggaattgca
     3361 atcttggtat aatgtcgagc ttagtgatcg tgaaggatat attcattatg ctgaacgctt
     3421 gattgccgat ggagcgaaaa atgttctcct ttctctagga ggagagggag gaatattttt
     3481 aaataaggat atcgtttatt ctgggaattc tcctaaaggt aaagtagtca atacagcgtg
     3541 tgctggtgac actttgttgg gaacatttct tgcaggatat ttaaaaggtg aacaggtaga
     3601 tattaatttg aagaaaagtt tagcagctgg aagttcaacc gcttttcgta aaggattaac
     3661 agatttttcg gatgttgcag agttggaaaa acaaatcgaa atcaaagagg gagcgagtaa
     3721 aaaatgacaa cttacaaaat tgttgccgca acaggatgtc caacaggaat tgcccacact
     3781 tatatggctc aagaagcatt ggagcaggca gctaagcgca aaggggtatc tatcaaagtt
     3841 gaaacccatg gtcaagtagg gatagagaat gaattaacac cagaagaaat aaaaaacgct
     3901 gacgctgtaa tcattgcagc tgacaaagat gttcaagcag aacgtttcgc aggtaagcgt
     3961 gtaatcgatg tgcctgtcag caaggggatt aaagatgctg accaattgat cgaagatgcg
     4021 ttggctggta aaggttcaat cactgcagaa aacaaaacag ttgacgctgc tggtgagaaa
     4081 gcggagatgg gggttgcagc aggcattggt cacagtattt ataaaaatct catgaacggt
     4141 gtttctcata tgctgccgtt tgtagtggca ggtggggtaa ttattgcaat ttcgtttgcc
     4201 atctggggtg tgtactcttt tgatcccaat gatcctactt acaatccgac tgccgctatg
     4261 ttaaagggtg ttggtgatgc ggcaatggga atgatggttc ctattttagc agcttatatc
     4321 gctgaaggaa tcgctaaacg tcctggtttg gttgttgggt ttgttggcgg actgttagcc
     4381 aattctggcg gagccggttt cttaggcgga atttttgcag gtttcttagc aggctacttt
     4441 atcttactat tgcaaaaggt catgaaaggc ttgcctaaac aattggatgg tctgaaggcg
     4501 atcttcttat atccggtgat cggtgtcttg tttgttggtg tagtcatgtc tttattagca
     4561 aatccaatgg aagcagtcaa ccaaggaatg atgaatttct tggcaggatt ccaaaattct
     4621 aatccattaa ttttagggat tatcgttggc tgtatgtcag catttgatat gggaggtcca
     4681 gtcaataaag cggcttatgt tacagggact gcgttgttgg cacaaggtaa ccatacattt
     4741 atggcagggg tttcggcagc ttgtatcgcg cccccattga ttactggatt tgccgcactg
     4801 ttttttggaa aatatttcga cacgaatgat cgcaacgctg gtttagtcaa ctttatcttg
     4861 ggctcaacgc acattaccga aggagcaatt ccatttgcgg ctaaagatcc gctgcgtgtt
     4921 ctgccaacta tgatgctagg ttcatcaatt gctgctgtac tgacatatat gtttggtgtc
     4981 caagttcccg cacctcatgg aggttttcta gtattacctg ttgttactgg taaggttgca
     5041 tgggtagggt cgattttact tggttcaatc gttggcggag taattttagg gttgatccaa
     5101 aaacgtagtg tagaaaaacg tgaagcagaa gcagctgctg ttgaaatgaa agaagcaacc
     5161 aacaactaaa ggttattcta gaaagaagga tttcaaatga ctttgatcaa aacaaataaa
     5221 gtttttatga ataaaaattt tacgacgcaa gatgaggtaa tgaacttcct tgcagatcag
     5281 tcaattgtct tgagcattag tgaagaccga gatgcagttt acgaaaaatt gatggaaaga
     5341 gaagaagaaa gcacgactgg tatgatggat ggttttgcca tccctcatgc caaagatgct
     5401 agtatcaagg atgccgatat tgtgattgtt cggttaaacg gtaaggtcga ttggaaaagt
     5461 ttagatggag aaggaacgga ttttgtaatc gctctgttta ttccagattc agaggctgga
     5521 acaacccatc taaaattttt atctcaggta gccagacttt tgatgcacaa agatgtgaca
     5581 gaaggtctga aacaagccca gtctgctgaa gaaatagctg aacttttaaa ccaaaaatta
     5641 gaagaaaact aaaaaggaag tagtgaaatc atgcctttaa cagaaaaaaa gaaaacttat
     5701 attcgtcaac taagtaatga aagtggcatc atcagtgcgt tagcaatcga tcaacgtggt
     5761 gccttgaaaa aaatgatcgg taaatttcaa aataaatcag caaccgatga acaaatcatc
     5821 aatttcaaaa aaatcatttc ttctgaattg actccatatg cttcagcaat cttattagat
     5881 ccagaatatg ggttgccggc agccgaagtt cgcgataaaa atgctggtct gttgttggcc
     5941 tatgaaaaaa ctggttacga tgcttcaact ccaggacgtt taccggatat tttatctatt
     6001 tggtctgtaa aacgtctaaa ggagcaaggg gccgatgctt gtaagttctt gttatactac
     6061 gatgtagatg aatctgatga catcaatgac caaaagaagg cgtttatgga gcggattggt
     6121 tctgaatgtg ttgctgaaga tttgccattt ttcttagaat tggtttctta tgatgccaac
     6181 gatcctgata cttcttcgaa ggaatatgcc ttgaaaaaac cgcataaagt aaatgaaatg
     6241 atgaaggaat tttctaagcc tcgctatgga gttgacgttt tgaaagtgga agttcctgtt
     6301 aatatgaact atgtggatgg ttacactaaa aatgaagttg cttataccaa agaagaagct
     6361 gctgcttatt tcaaagaaca atccgagact accaacctgc catttatttt cttgagcgca
     6421 ggagtaactg ctgaattgtt ccaagaaacc ttgaaatttg caaaagaggc tggctctaca
     6481 ttcaatgggg tactctgtgg gcgcgcaact tgggcgaatg gtgtagaacc attcgttact
     6541 aaaggtgaat atgctgccaa agaatggatg cagacacaag gacgcaagaa catcgaagag
     6601 ctgaataatg ttttgaaaga aacagcaaca gctattaaat tgtaagaaag ctttaactat
     6661 aaccttaagt ttgtaaccta atgattttta ataatcaatt tgagctttta aataaagaat
     6721 tggaagcgtc ccgacgcttc ctttttcgtt tgtttttaag gcatgatagg ttctatgttg
     6781 tcgttcaata agcgcaagaa ggtcagcatt tttttcttag agtatgcaaa attgcttttc
     6841 tacccaatga tttaaaacgg agaggcaccc ttggatatgg tagatggtga agtaacgcat
     6901 ttcttctgtg atcacggttt ttttgtcttc ttgtagccag atttttaaat aattttcttc
     6961 gatcagatta tttaatctct tttgaaattg gatatctccg tggttgatga cgaataactt
     7021 gtatacgaac gtattcgtat agacatagtc gatcaattga gcgtatagat cctcgtagct
     7081 atgtgtcgta tcgtaggaaa tgatttcatc caagtcttgg aaaaagctat tttccagttt
     7141 ttcataaaga tcatagatat ccgtataatg tgtataaaat gtcgaacggt gaagatcagc
     7201 agtttcggtc agttcacgga tggtgatgtt ttgaattttt ttatctacta ataattttga
     7261 taatgcttct gctaacgctt tttctgtttt tttgacgcgg cgatcctctt ttttcatttt
     7321 gcagcctcct aatcaacata atcaaggaat tgtgtcggtt atccgacaat ttcaccgcta
     7381 tctgaacata gataaccgac accttgtcta ttataatata agtatagcat atcaatattt
     7441 aaagaatagg agaataagtg atggaattag cctttaagca actggaaaag aaagactaca
     7501 aaaaagccat tcaattcgcg attaaaggta tgcactttga ttggtatacc aagaacaaac
     7561 tccttttgaa tctttatgga agatactttt ggtatctaga attgaatcgc gcgacacaaa
     7621 tcattgcttt atatgcggat gatgagttgg caggtgtttt attagcagac atcaaaggaa
     7681 aatcaaaaaa gcatcattca tttagtcaaa agttatatgt taaactattt gatttcttac
     7741 aaaatgcatt tgtgaaagac agcaaaggcg ggtatgacga caccaatgac gaattgcttt
     7801 cagactattt aaaaaaacat tccccagatg gagaaattgt ctttttagct gccaaccctg
     7861 atttaaagat caaggggatc ggtagtaaat tattaaaaga atttgaacgc cgagagcaag
     7921 gaaaagaagt atttctgttc acggatagcg gctgtacgta tcaattttat gaacgacgcg
     7981 gtttcgaacg gaaaaaagaa aaagataacg tcatgaagat ccttaataaa gaagtagatc
     8041 tccgctgtat gttatatagt aagatcattc agtaatgttt tttactatca gaaagcagaa
     8101 gtaccacgaa ggtcaatgga aaaagcgagt aattgatcca tttagatgaa gataggtgta
     8161 gtctcaaaga tattagctgg caaagatatc cccagttaaa aat
//
LOCUS       DAZKNO010000122         8198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000122 DAZKNO010000000
VERSION     DAZKNO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.124"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..709
                     /locus_tag="ACG8MY_002039"
     CDS             116..709
                     /locus_tag="ACG8MY_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0431213.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKEAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDEYAIQPHYTNKENFDLGITETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            725..1624
                     /locus_tag="ACG8MY_002040"
     CDS             725..1624
                     /locus_tag="ACG8MY_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0431214.1"
                     /translation="MTPTQFKAKTRNFATEKHLNPQIVQRYYMMEKLLERIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTADPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERINEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKVERKQNFKRSQQMNQEMER"
     gene            1627..2112
                     /gene="ssb"
                     /locus_tag="ACG8MY_002041"
     CDS             1627..2112
                     /gene="ssb"
                     /locus_tag="ACG8MY_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0431215.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTATFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            2157..2390
                     /locus_tag="ACG8MY_002042"
     CDS             2157..2390
                     /locus_tag="ACG8MY_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431216.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGRDNSFWRLTKVTIAATAVILTAILPLYM"
     gene            complement(2549..2671)
                     /locus_tag="ACG8MY_002043"
     CDS             complement(2549..2671)
                     /locus_tag="ACG8MY_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFQ0431217.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            2826..3086
                     /locus_tag="ACG8MY_002044"
     CDS             2826..3086
                     /locus_tag="ACG8MY_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431218.1"
                     /translation="MDKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3529..3735
                     /locus_tag="ACG8MY_002045"
     CDS             3529..3735
                     /locus_tag="ACG8MY_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0431219.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     QTCGKKYTLSYGDYVLIDEKGKKH"
     gene            3735..3986
                     /locus_tag="ACG8MY_002046"
     CDS             3735..3986
                     /locus_tag="ACG8MY_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431220.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            4002..4439
                     /locus_tag="ACG8MY_002047"
     CDS             4002..4439
                     /locus_tag="ACG8MY_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431221.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            complement(4550..4786)
                     /locus_tag="ACG8MY_002048"
     CDS             complement(4550..4786)
                     /locus_tag="ACG8MY_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431222.1"
                     /translation="MKQNTSITKNTTKFKCTAALCRIIISPTNSTNNKGIILFKWITF
                     FLLSYRALPVYFASPKRRGRLERLVRNKNHSLSY"
     gene            4884..5063
                     /locus_tag="ACG8MY_002049"
     CDS             4884..5063
                     /locus_tag="ACG8MY_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431223.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQKLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            5404..5667
                     /locus_tag="ACG8MY_002050"
     CDS             5404..5667
                     /locus_tag="ACG8MY_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0431224.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            5661..6011
                     /locus_tag="ACG8MY_002051"
     CDS             5661..6011
                     /locus_tag="ACG8MY_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0431225.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(6035..6649)
                     /locus_tag="ACG8MY_002052"
     CDS             complement(6035..6649)
                     /locus_tag="ACG8MY_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0431226.1"
                     /translation="MRVAYIRVSSIDQNEQRQVEEMKKFGAERIFIEKQSGATITHRP
                     VFQEVLDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSTVNHE"
     gene            complement(6990..7193)
                     /locus_tag="ACG8MY_002053"
     CDS             complement(6990..7193)
                     /locus_tag="ACG8MY_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431227.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(7203..7625)
                     /locus_tag="ACG8MY_002054"
     CDS             complement(7203..7625)
                     /locus_tag="ACG8MY_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431228.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            complement(7794..>8198)
                     /locus_tag="ACG8MY_002055"
     CDS             complement(7794..>8198)
                     /locus_tag="ACG8MY_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0431229.1"
                     /translation="ICAKVRVSDRKEVANEFKEVYRSKSKEEALTSLKKFIDRWKHKY
                     PSMVKSLSNNDYLFTFYDYPESVRHTIYSTNLIEGNNKQLKRSFKKKEQFPTEKSEEK
                     YLVIQFNRYNEKNMNHVHRGFGQTTREDWFKS"
BASE COUNT         2827 a         1331 c         1508 g         2532 t
ORIGIN      
        1 aaccagcagc ggattcaaaa agaaattgaa ttagatcgat aaatttcttt cttgttaata
       61 taaaactaac atgttatagt tatgttagtt ttatattaac tttaggggaa aagcaatgaa
      121 agacaaaatc cagtgtattt ttgataaaca taatgggaat ctatccttga aggatgctag
      181 aagtgaagga ttatcgccta tgacgttaga tcgattagcc gaaaaggaca aaatagaaaa
      241 ggtagcacca ggtttctata ccttaaaaga agcgttcgtg gatgaattgt atctcgctca
      301 aagtatctat tccagaggga ttttttctca tgagactgcg ctcgatcttt ttcaagtagg
      361 aacgtatatc cctaaaaaga tcaatatgat gtttcctaaa ggatacaatg tttcaaagga
      421 gaaattagac gagtatgcta ttcaaccgca ttacacgaat aaagaaaatt ttgacctggg
      481 aatcacagaa acagaaagct tttacggcaa taaaatttta gtctatgatt tagagcgaac
      541 gttatgtgac atgtggaatc caagatacaa agcaagcgta gaggtaaaac aagaagcatt
      601 aaaagaatat atgacaagta ccagtagaaa tacaaataag ctaagaagat acatggatat
      661 tttaaaatca ccaaaagaaa tgactttgta tatgttgcca ctttattgag aggagtaaag
      721 gtgaatgaca ccgacgcaat ttaaagcaaa aacgagaaat tttgcaacag agaaacattt
      781 aaacccacaa attgtccaaa gatattatat gatggaaaaa ttattagaac gtattgttga
      841 atcgccttat catgatgatt ttgtattaaa agggggattt ctgatcggat caaaatacgg
      901 gttagaaaac cgttcaactg ttgatattga tacgacttat cgaaattcga aactaacaaa
      961 agaaaagtta gaaactgttt tttctgaatt aacagctgat cccacaaaag atggaattcg
     1021 atttgaatta aagggattaa cagagacgag ggaagctgat tattacccag gttttcaagc
     1081 aaagtttatt gcttatcttg agaatgcacg tgtaccattt aaattagata ttacttcagg
     1141 tgattctatt attccagcgg cattgatgta tgaacacaaa ttaatgtttg aagataaaac
     1201 gattcatatt ccagcttatc ctacggaaca aattctagct gaaaaattac atgccacgtt
     1261 tagttttgga aaagataatt cgcgcatgaa agatttttat gacctgtata tgattccgaa
     1321 attagaacgt atcaatgaga aaaatttgta taaaagtgtt caaggaacat tcaaggaaag
     1381 aaataatcaa tcggtgttta agcatttgta tgaccaggaa atgcctcaat tggtgaagga
     1441 tgaggggttg agggaaaagt ggaaaagata ccaacaagaa aactattttg ccaaagacat
     1501 ttctttcgaa tctacactaa cttccgttga ttccctgatg caatcaattg tagagcaaga
     1561 gaaggtagaa cgaaaacaaa attttaagag atcacaacaa atgaaccaag aaatggagag
     1621 ataaagatga ttaataatgt aactttagta ggacggttaa ccaaagatcc agatttacgt
     1681 tataccgcaa gtggcacagc agtagcgact tttaccctag ccgtcaaccg aaatttcgcg
     1741 aatcaaaacg gcaatcgaga agccgatttt attaactgcg tgatttggcg caaaccagca
     1801 gaaacaatgg cgacattagc taaaaaaggc ggcttgatcg gtgttgtcgg acggattcaa
     1861 acacgaactt atgacaacca gcaaggacaa cgtgtgtatg tgactgaagt tgtagcagat
     1921 aactttcaac tattagaaag taaagcagcg acagagagcc gtgcgcacgc tgatcaatcg
     1981 tccaccagtc caagcaccgc cacctttgaa caacgggata cagcgacgcc taacaacaat
     2041 ggattgaacg cttcacagaa tccatttggt ggacaatcaa tcgatattag cgatgatgat
     2101 ctaccgtttt agttcgagaa agaaggagta agcatggcaa aaaaaatagt ctgacgatgg
     2161 atctattgat acccgtaatt gcatttatag acgggatttc tttgggcgtc actgaaaatc
     2221 ctttggtaaa tagaattgcg aaaggatcac tatttcttgt attcgtgtat cttgtatgtt
     2281 cttatctttt aaaaagagca ggaagggata attccttttg gcgattgact aaagtcacaa
     2341 tagcagcgac agctgttata cttacggcta ttctaccgct ttatatgtag ggttaacggg
     2401 aaatcgttga acttcttgcg aaatattgct ataatgaaca tgaaaaagag gagttgcgct
     2461 aacaacccct cattaacagc accgattaaa gaacggtggc agaatattag ttatttggaa
     2521 ataaccgctg acctgctaaa gtggacggtt atttcttttt gtcattttta agcatattta
     2581 caatcaaact aatcaaagca atggtaaaca taccaaaacc aagaatggtt tgaattgtct
     2641 catatgcgga caaaaggctt actcctttca tagattttag cacttacatt cataagcacc
     2701 accacctttc cagaagtcgc caccgtcttc aaactttact gtcttgacta ttatagcata
     2761 tctaaaatcg aataaaaatt ctcaattttg gttgtttttg ctataatttt agcgaggtga
     2821 ttgtgatgga caaatatgtt ggtttgcttg ataaaatccg agtaataaaa acacaaccat
     2881 tattggttcg ctttacttta caaacaattc atgaatcgat taattgtgta gttgcagata
     2941 ttgaaattat cgataaactg ctaattatgg atgatggaaa atacaatatt gccgtcacag
     3001 gtcacttcaa taaacgaaat cagctagtta ttgctagcat gtatgttaga aatcccgatc
     3061 attttacgcg atcgatgggc atataaaaga agagttgtag tcctaaatga ggattgcagc
     3121 tctttttgtt ttacatagaa acgggaacag gaaaaggcct gtttaggaag gcacaagcgc
     3181 cttctcaatt gaaaaacctt tctctcactc caaccgccac cgccgttcga gaagaaagga
     3241 agtgaacgtt gcacgttcac taaaataagg ggtcttgcaa agcaagacaa aataggaaaa
     3301 acttcctctc accaaaaggt tcgttcctgt tcgctatctc tttattgatt tgccagaccg
     3361 gcaacaagtt gccttttatc accaaaaagc gacaaggaca agggtaaatg gcacgctgcg
     3421 cgcccttgtc cttgtcgctt ttcgttgatt cattctggcc aacaaatcaa taaagaaaga
     3481 actaacaaaa ggaagtaaac cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa
     3541 tttgattatt ggggtgttcc cttcagcgaa ttattcccaa actatcatgc accacatact
     3601 gtcgaatgcg attgtggaga aagagcgaaa tgtatcaaaa gctatcgttt gtatcaatgt
     3661 caaacatgcg ggaaaaaata taccttgtct tatggcgatt atgttttgat tgatgagaaa
     3721 ggaaagaaac actgatgaaa acacaacaca aattattgac tgtaaaagaa gtcgttcaaa
     3781 ttttggtgga caaagaagtc atttctgaaa aagtagcaga agctttactt attccgcggt
     3841 actggacgat agaaacccct ttagtagagg atgcaccgac tatgctattt gaagacctag
     3901 tgaagattgc aggatatgaa ctgcattatc aagaaaacat ggaaacttat gaaaacgaat
     3961 attggttaac caaattagag aactaaaaag gagaaaaaaa tatggctcaa aattactatg
     4021 acgaatttgt caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc
     4081 tatacaacga aacacgtgtt ccaaagaaac attataaaga aaaattgtcg gtagctgtgg
     4141 aagaaatgat tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacactgg
     4201 aagaactgaa aaagcaaaat gcaaaatggt ttttccaagc cttactatgc ttggaagtag
     4261 gcgtcaaacc aagcacaatc aaaccttctg aataccaagc tttagaacta acgtatgcga
     4321 aatttatcga aacgaaaaaa gccaagactg tttcaagtga atggttagat tattttgaaa
     4381 atatcaataa gtatggcgca tattacacca tgaaaaagga agacaacgaa aatgaataag
     4441 gtgatttatc aactaacaac ggattccata tcaacgattt gaaagaaaaa aaggtcaata
     4501 atcatttata ccgaaaagtg agaatgtgtg aaataaggac aaaaaaagcc tagtaactaa
     4561 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtgatgcga
     4621 aataaacggg caaagcccga taactcaaga gaaagaaggt gatccatttg aacaagatta
     4681 ttcccttatt gttagtacta tttgttggcg aaataattat gcgacatagt gcagctgtgc
     4741 atttaaactt cgtagtattt ttggtaatac tggtgttttg tttgatcttt tggcgctttg
     4801 tgttagccct tgtgagtcta atattcaagt tgattgcagc catatttttc atagggttat
     4861 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtta
     4921 cgttggatga aaagagacaa aaagaattgc agaaactaaa gcagaagtac ggaaaaagtg
     4981 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
     5041 atgtaccagc actcaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
     5101 ttaattgttc ctactattta gaatattttc tatatccttt ttattccatt ttgtatcaat
     5161 aactatgaaa tctttaaagt atccagaaga gattattttt agatcaattt ttttcttacc
     5221 tgattctaaa gattttgttt tcttaaacat attatgattt cctttcgact attttcatat
     5281 aaatgttaac atagtttatg gattcatgga atgttaatct aatttaatag aaaaggataa
     5341 ttaagtatat acgttttttc ttatgtatgt tatactatgt atatacagaa agaggtgtct
     5401 actatggaaa tgttacctat aaaaaaatgg ggaaatagta atggccttag attgccaaag
     5461 tatattatgg aatatcttgg aattcataca gaagacaaag tgaagataat acaagaagaa
     5521 gtaaacggtc agaagcggct aattatcgaa gcggctaata tagaaaatga attaacaatt
     5581 gaacagttat ttgaaaatta cactgatgaa agaaatcatg tgacgattca aaatttaggt
     5641 gaagcagtag gtaatgaaaa atggtgagaa aacctagaca aggcgatatt ctattattga
     5701 atacagcacc tcgatctgga cacgaacaga caggtaaaag gccttatatt gttttaagtc
     5761 atgatattat tgcggattat agtaacgttg ttacagttgc tccaatttct agtacaacta
     5821 gaaactatcc gctatatgta aatataaact cagaatatga aatgaagaca acaggaaaag
     5881 tattattaga tcaactaacg acaattgatt atgaagccag agagtgtatt tttttggaaa
     5941 aagcgcatga tagtttagtt gaggagttat tgatgaaagt tagaacagtt tttcaaaaag
     6001 taagcaaata aaatagaaac agcagcatga tcaactattc gtgattgacc gtgctgtctt
     6061 tttttattct atatacggtt tgtctcgtaa cattgtagtc ttttgcaatc ttagcgatcg
     6121 caacaccttt ttttagatcc tcaacgatgt ttttataaac aagacgacgt tggggatctt
     6181 ttgcattggc actgtatagc ttaggacgtc ctttataaac accgttcgct ttcgcaatct
     6241 taatgccttg tgcctgacgt cgtttagatt cggtacgttc ttgttcagcg atcatggcca
     6301 aaatttgaat gatcaaatct ttaatgaatt tatccaggag aggattgcca attgcttcgg
     6361 ccatgatcgg tagacttgta atgatcagct gcacattttt cttcttcaag taattgactg
     6421 aagcgatgat ctcgtcgtaa tttcttccaa gtcgatcaat agcttccaca ataaaaatat
     6481 cttgatctcg gacgtaatcc aatacttctt ggaaaacagg tcgatgagta attgtagcgc
     6541 cagattgttt ctcgatgaaa attctctctg caccgaattt tttcatttct tcaacttggc
     6601 gttgttcatt ttgatcgatc gatgagacac gtatgtatgc aactctcaaa tttttaccta
     6661 ctttctgcaa ctatttaaaa cacaccctaa tcatacatga gaaaaccaga tttttcaatg
     6721 tttgtcattt ttctagataa atggcataaa ttttcagcgc taagtgacaa aagcaactaa
     6781 tttttcatga tagattttta aatatctcaa tctatctcca aaattagttg cttttgtagc
     6841 tatagttgac tatgaatatg acaaataatt agaaaaaact gtgccaatta aatataaaaa
     6901 tgacagaaag tgtccagaaa tgggtgattt tatgaacact ttcaattaga aaaaaagaat
     6961 gtcgactaga cactcttttt ttaaaatcat tatttttctt tttgatatgt tttttggtct
     7021 cttttttttc ttttttcttc agaatcttct agattagaaa ttgatttttg aattttttgt
     7081 gtctcctgta aagttcgctg aacttgtaaa gctgattctt ctttcggtaa attaatttta
     7141 cttagaatat cgctatcttg ataaaaattt ggtgtgttca caaattctgg cataaaaaat
     7201 ccctactttc tagttaaggt tcctgaataa gtcgcaatcc aattgtttcc agctgattta
     7261 taactttgta aatacaattt tccatagtac caataaccat ttcttttttc tttccaattc
     7321 cgtgattgag gaggtctaga aggccaacca actttatcaa aaatcatcgt tcctgtcact
     7381 cgtatcgttt tagaagctgc tcgagcaagt aatggagaaa ctattaaata attttcagta
     7441 acagttaagt cgttagttgg gttaccaatg gctattccgt tcctatctaa aggagttaat
     7501 tcttggttat tatctactac agtagttggt tcatttgtac tatcagcaaa tgtaggaatt
     7561 gtagttccag ttacactaat tccaatagtt aaaataccta aagataaaat aaacttattt
     7621 ttcattgtct atcacctcga aatcaaaaat aagaaaacgt tttcttaata aatcttaaca
     7681 gataaatcaa gttatatact agaaaattca taaaatatat ttttaatgtc ataatcgttt
     7741 aaatttggga gcgtcaataa ttttgtgtaa aagtatatcc ttggtaaagc agattagctc
     7801 ttgaaccaat cttctctggt tgtttgtcca aaacctctat gtacgtgatt catgtttttc
     7861 tcgttgtaac gattgaactg aatcacaagg tatttctctt cagatttttc cgttggaaat
     7921 tgttctttct ttttgaaact tcgttttaat tgtttattgt ttccttcaat cagatttgtt
     7981 gaataaatcg tatgcctaac actttctgga taatcataga atgtaaacag atagtcattg
     8041 ttgctgagac ttttcaccat tgatggatat ttgtgtttcc acctatcgat gaatttcttt
     8101 agagaagtta aagcttcttc ttttgatttg gaccgatata cctctttaaa ttcattggcc
     8161 acttcttttc tgtcactgac tcttactttt gcacatat
//
LOCUS       DAZKNO010000123         8195 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000123 DAZKNO010000000
VERSION     DAZKNO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.125"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..2218
                     /locus_tag="ACG8MY_002056"
     CDS             119..2218
                     /locus_tag="ACG8MY_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0431230.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2414..3277
                     /locus_tag="ACG8MY_002057"
     CDS             2414..3277
                     /locus_tag="ACG8MY_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0431231.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3382..3855
                     /locus_tag="ACG8MY_002058"
     CDS             3382..3855
                     /locus_tag="ACG8MY_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0431232.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            3951..4838
                     /gene="xerD"
                     /locus_tag="ACG8MY_002059"
     CDS             3951..4838
                     /gene="xerD"
                     /locus_tag="ACG8MY_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0431233.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            4857..5228
                     /locus_tag="ACG8MY_002060"
     CDS             4857..5228
                     /locus_tag="ACG8MY_002060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0431234.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            5225..5995
                     /locus_tag="ACG8MY_002061"
     CDS             5225..5995
                     /locus_tag="ACG8MY_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0431235.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            5979..6548
                     /gene="scpB"
                     /locus_tag="ACG8MY_002062"
     CDS             5979..6548
                     /gene="scpB"
                     /locus_tag="ACG8MY_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0431236.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            6554..7270
                     /locus_tag="ACG8MY_002063"
     CDS             6554..7270
                     /locus_tag="ACG8MY_002063"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0431237.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      7352..7477
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7600..>8195
                     /locus_tag="ACG8MY_002064"
     CDS             7600..>8195
                     /locus_tag="ACG8MY_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0431238.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQR"
BASE COUNT         2986 a         1404 c         1737 g         2068 t
ORIGIN      
        1 gaaggaaatt cattcattat ttttaagtaa atcctaaaaa taagctattg gcgtgacgta
       61 tagaatatga tagattaaat ggtagcgtaa acctagtaaa ggagccgatc gctaagtgat
      121 gaaaaaatgt ccaaattgcg gatcgaaaaa aatggagcag aatactatct gtccagactg
      181 cggtcatcaa ataactgcag aagatcagca aataaaagaa tcaattgaag aaacaaatac
      241 agatgcagct atgcaggata aatcaactga agatgcagtc tttcctgata cagaattaaa
      301 cgatccaatc gaatggtcgg aattgaaaga tttacctcta gaatctgtaa tggaattatt
      361 cgaaaaagca gaaccagaag acgaagaacc acctgctcac gatcaagtaa ctaatataga
      421 gaaaaatgaa cagaagaaag caactgttca tgagacttcg gaagaatcag gaaagaatca
      481 agaagaaacg cttgaatcaa aacaaaaaag aagagtagct gaactgaaag aaatcgtcga
      541 taatgaggaa gaaaattcga tactatccgc ttatatcaaa gcacatcgtg aagatacaaa
      601 agaagagcac gcgaaagagc tgctcaaaat gatcagtgaa aaaattgcac aggaaaacaa
      661 agtagagcca gaaaaggaac aagaaactga aaaagactct gatagagaaa acgccacagg
      721 taaagaaaca gtaaaaataa aagacgtaaa aacggatatc acagaaaaaa ctgaagtatt
      781 tactgaaaaa caattagaaa aggaaaaaac gaataaactg ccggaagcag agaaaaaaga
      841 aatactcgaa aacacagaca aaaagtcaac agagcccgat gaaataaata gtgaatccga
      901 tgctgaaaat caatctgtaa gcaaagaggg tactgatctt gaagcaaaaa tagaaaaaga
      961 agagaaccag cctaagctgt ttgaagcaga agcgaaatca ataacaatcg tgccagatac
     1021 tgaaaaaaca catcaagaat caaacttaga aaaaacagat ccagataaag cgcaaaagaa
     1081 aaaatcaaaa aaagtacctt atctcgtttt agcagcagta cttctactcg gtggaggaag
     1141 ctgggcttat tatgaccatc atcaaaaagt agaagccgaa gtagcagcag aagcaaaaag
     1201 acagcaacaa atggaagaac ttcatgagaa acttgctgcc ttttatacag atgatcacca
     1261 tcaatttatt cgtgcagata tgattcatca agatttatca aaactaaaaa atgatttatc
     1321 cagtcttaag aatgaaaaag gatattcaga gttagaaaca acttaccaag atatccaatc
     1381 taagatccga gatatccaaa aagtgaatga gtggttcata gcacctgcca tcgtcgatga
     1441 tcatctagca gatgacatca agttgaaagc agataaagag atccaagcaa tcgaaacgga
     1501 taacacagat tttggaaaat tgattcaaca agcacaaaca gaagctaaaa atcaatatgc
     1561 tgaattgcaa acagctaaag aaaaaacagc tatagtcttt aaagacggaa aagtagtgga
     1621 ttcagcaaca cgcgaccaat acaagacggc aaaagaagca gtagaaaaag tgaaaaatac
     1681 agatctatct gctcagttga atgaacaatt gaaacaagta gataagatgc tcactgataa
     1741 agagaaaaaa gaagaagagg aaaagaaaaa agcagaagaa gcgaaaaaag cagctgaaga
     1801 acaagcaaaa caagccgctc aagcagcagc tgcagctcaa gccgctcaac aagctgcggt
     1861 cgcacaagca gcacagcaag aagcggcatc ctcgacccct tcctcttcaa actcagcgaa
     1921 tcagccaatc aaatctgcca gagcaagtga tattgccgat gcatctaatc cagcatggac
     1981 gtgggcacca ggtgtccaaa gcaaagtgat tgctacatgt atccaacgtg gatatatcgt
     2041 tgaaggaggt tacagattag aaaaagccag aatcgaaaat ggggaaggct attataatct
     2101 atatgccact tcaacaaaat ctgcattgat gaaaggcatt ggagaaagtg ctttgccatt
     2161 ttacatcgta accatcaact gtaaaactgg atggttcgga ggaaatggga gcaattaatt
     2221 ttaggcaaat gaaagaggct gtgagcctgc cgtttaacac cgtttattcg aaaaagggga
     2281 gtatgacaca actgttgtca tactcctttt ttcacctttc caaaccaagg ctcagctatt
     2341 caagtagata acattatgtt atgatagcaa agaaatgtat taagaaaacc tttcactata
     2401 gagggagaaa aaaatgaacg atttattagc aactatattt acaggtatta ttattgatga
     2461 aaatgaaaaa aactactttg tccaaaaaaa cggacaaacc tttggcttaa gcaaatcaga
     2521 aggtgaacac aaaatcggtg aagcagttga aggattcggt taccaaaatc aaaaacatga
     2581 aaatcggatc actacacaaa ttcctaaaag ccgtatcggt cattacggtt ttggaatcgt
     2641 tactgctaca cgaaaagatt taggtgcgtt tgtcgatatc ggtcttccag ataaagactt
     2701 tgtcgtatct cttgatgaat tgccgacaat gacagagcta tggcctaaaa aaggggatcg
     2761 cttgatgatt tcccttagag ttgatgcaaa agaccgtatt tggggcagtt tagcagatga
     2821 aaaaatattc aaatctttaa gcaaatatgg aacagaagaa atgaaaaaca aagatgtgat
     2881 aggaacagca taccgcttga aattaacagg tacgtacgtt ttaacggaag atttctatct
     2941 tgggtttatc catccttctg aacgatataa tgaacctcgt ctaggcgagc agataaaagg
     3001 tcgagtgatt ggtgtccgac cagacggtgt actgaatctt tcattgaagc caagagcata
     3061 tgaagctata tcagatgatg cacaaatgat tttaaccatt ttagaacgtt ctgcagatca
     3121 gcaaatcaac tttacagata aatcggatcc agaagaaatc atgaaagctt ttggtatcag
     3181 caaaggagca tttaagcgcg cattaggtaa tcttttgaaa caaggattga ttacacaaga
     3241 aaacggtgtg acaaaactag tcaaaaaatc taattgagaa tcccttgcat gtttttctta
     3301 actagcttat aataatttta attaacatag tgttatatct ttttttattt gtaattatta
     3361 taaattgttg gggttgagta aatggtcatg aatgctattt cggcgatgaa aaaaacaaaa
     3421 cagcagctac atgaagcggg gtttaagctg acaccgcaaa gagaagctac tctgcttgtg
     3481 cttttggaaa atgagaaaga gcatttatct gcagaagaaa tcttcttttt agtgaaaaaa
     3541 aagagtccgg aaattggact tgctactgtt tatcgaaccc ttgaaatctt gacagaattg
     3601 aaaattgtcg ataaagtaag ttttaatgat ggattagccc gttatgattt gagaaaagaa
     3661 ggcgccaaac atttccatca tcatttacta tgcttgaact gcggcacgat cgaggaagta
     3721 gaagaggatc ttttacttga agtggagcaa gtagtagaag aacgttatca ttttttagta
     3781 aaagatcatc ggctaacgtt ccatggaatc tgtcaggaat gctatgaaaa agaaaaaaga
     3841 aaccagtccg tctgagtgtc tagacggctt tttctttgct cagattatgg aaaaagacta
     3901 tcagaatgaa gcggtatctg atatgataga gaggcatgag ggggattatt atgaaagagc
     3961 aaatcaacga ttatctccac tacttgaata ttgaacgggg attgtccgcc aatacacgac
     4021 aaagctatga acgtgatttg gaacagtact tgcattttct acaggagcag aaaattgctt
     4081 catggcagga tgtcgatcgc tttttagtca tccgttttct ggagaagttg catgaagaaa
     4141 aaaaagcgac agcaactgtt actcggatga tctcaagtct cagaagattc catcagtttt
     4201 tacggcaaga gcgtatcact gatcatgatc cgatgcaaca tatcgatacg ccgaagaaag
     4261 cacagaaatt accaagtaca ttgactctga cagaagtaga aaagctgatt gaagcacctg
     4321 atacgacaaa aacgttaggg ataagagatc gtgcaatctt ggaagtcatg tatgctactg
     4381 ggatgcgcgt cagtgaattg attggattga aattgggaga tcttcatctt tctcttggac
     4441 tggtacagac agttggaaaa ggagataaag agcggatcat tcctttaggt gattatgcga
     4501 tccaatggct ggaacgctac ttagacgaag cacgtccatt acttgtaaag gacgcatctg
     4561 aaatgcatgt atttgtcaat aatcatggga aaggtctttc tcggcaagga atctggaaaa
     4621 atctgaagca gttagtacga gaggctggga tttacaaaga agtaacacca catacgctgc
     4681 ggcatagctt tgccacacat ctgctagaaa atggagctga tttgcggaca gtacaggaat
     4741 tgttaggtca cgcggatatc tctacgactc agatttatac acatatcacg aagaaaagaa
     4801 tgacggatgt ttataagcag cattttccaa gggcttagtc ataggaaggt gagaagatgt
     4861 taacacatta ccggaaagaa aaccgtaaaa ttgctatggg actattgagt ttccataaga
     4921 aactgacagg taaaacttct ttgttgaagg agattgatac gtacgaatct gaagacgatt
     4981 atgaactttt tttctttacg ccagaaggtt caaccaacat tcaagggatt attggtattt
     5041 actgggcaac gaacgatcag ctgatcatcc atgacatttc tttgaatccg tcttatcgcg
     5101 gtgagaatat gggctttcaa atgttagatg aattacagga aaagtacccg gatgtctcca
     5161 tccgtgcaac tgaaatcaca gagccttaca ttggaaaatg gctagcaaag aaaaagaaga
     5221 aagagtgaat gagttgacgg agatcaatat taaattagat gtattcgagg gtcctctcga
     5281 tctgctgctt catctgattc agcaaatgga aatcgatata tatgatatac cgatcgctac
     5341 gatcacggag caatatatga attatatcca tacaatgaaa acactcgagc tagctgttgc
     5401 aggagaatac ttagtgatgg ctgctacatt gatggcaatc aaaagcaaga cattgcttcc
     5461 tgtccaagaa ttcgtcacag acgatgaaga attatgggaa gaagatcctc gagaagaatt
     5521 ggtcactcag cttttagaat accgtaaata taaatatgca gctgaaagac tgacagaaaa
     5581 agcagaggaa cgaagtcttt attacacaaa agaacctatg aacatcgatg ggttagcgga
     5641 tgctgaaaag ccgttgaagc gaggacaagt caaaaaaatc gatttgatcc gtgccatgca
     5701 gaaaatctta gaacgtaaga aattttctca aaaagtggaa aaaacgatca cgcccgatga
     5761 cacaacgatc gaagaacgaa tggtatttat cgaagagcgt ataaaaagaa atgcgcatgg
     5821 ctgtactttc gattcttttt ttgagtcacc ttctaaaagt gaagttgtca ctacttttat
     5881 ggctttgcta gaattgatga aaaaagggca gatcattgcg caacaaaaag aaaactatga
     5941 atcaattatg ctttatccag cagtaggaga taccgaacat gacgaaatta agtgaacttg
     6001 aagcccttct ttttattgtt ggcgaggaag ggatcagtaa tgaagaatta agcggactgt
     6061 taaatctcag cgaagcagaa gtcacaagtt tagtgaaaga attaaaagaa tcttacactc
     6121 aaaactcgca ctcagctttg catatttttg aaacggaaag ccattatgta ttaacgacta
     6181 aaaaagagct ggctccctta cttaagcgat atgcgcaagg gacatcaaac acattatctc
     6241 aagccgccgt cgaaacctta gcaatcatcg cttataaaca gccaatcaca agagctgaaa
     6301 tcgagcagat tcgaggagtt caggtttcag gagcggtaca gcgtctagct gcccataaac
     6361 tgattgaaga aaaaggtaga gtagaaggac caggtcggcc gatattatat ggaacaactg
     6421 gctacttctt agattatttt ggtttgaaat cattagaaga actgcctgat acacaagaaa
     6481 tggaaatttc acttgaagaa gacgtgtcac cagatctatt ctttgatgga atgaataaag
     6541 gagaataatt gtaatggaga gattgcagaa agtaattgcc cacgctggca tcacttctcg
     6601 tcgaaaagca gaagaattca tccttgcagg acgtgtaaaa gtaaacggac aagttgtaag
     6661 ggaattaggg ataaaagttg ggaaacatga cactgtggaa gtggatgaag tcccaattta
     6721 tcaagaagaa tacggttact atttattgta caaacctaaa ggagttattt ctgctgttgc
     6781 agatgacaaa ggaagaaaag ttgttacgga tcttttgccg atggtaaaag aacgtatcta
     6841 tcctgtaggt cgactggatt acgatacttc ggggatcttg ctactgacaa atgatggaga
     6901 ttttgctcag cgcctgacac atccaaaaca tgaagtggat aaagtttacg ttgcaaaggt
     6961 aaaagggatc gctacaaaat cgatgcttgc acctttgaca aaaggaataa agatcgaagg
     7021 acgaaaaaca tctccagcta aattccaaat tttatcagtg gatcctaaga caaaccacag
     7081 tatcgtggaa ttgacgatcc acgaaggacg taatcatcaa gtgaaaaata tgcttcaagc
     7141 agttggtctt cctgtgcaaa aactaaaacg tgaaaaatac ggagatctga ctttacaagg
     7201 cctacgtcct ggggattacc gtgaactgaa caaaaaagaa gtcagcagtt tgctgaacca
     7261 ctcaaaatga acacttactt tttataagtg ttagatgttg cgttttttga atagatatgt
     7321 ataatgaata tgtaaacaaa aattatataa ggttcgtctt caggggcagg gtgaaattcc
     7381 cgaccggtgg ttatagtcca cgacctattt tgcaaaaaaa tagctgaatc ggtgaaattc
     7441 cgataccgac agtatagtct ggataaagaa gatagagctt atttgacgac gcttgatcaa
     7501 ataactctaa ctctattttc cctggaaaat agagtttttt catttggaga gaaacgtcaa
     7561 aaagttcgct gaagaggagt cctaacagga ggatttcaaa tgaagaacac ccgtgtacag
     7621 aaaatggttg cagtagcgat gtttgctgcg atcgcattag ttttacagta ttttgctttt
     7681 ccgatcatgc cggcttttag ttttttgaag atcgatttta gtgatatctc ggtattgatc
     7741 agcatgttct tattcggtcc agcggccgga gttgcaacag cgttcttgcg ttcagtatta
     7801 catctgctga ctacaggttt ttctccggat aatttggtag gagacactgc tagtttctta
     7861 tcaacaacga tctttacatt cccgatttat tatttcttta gtgtaaagaa acagcataaa
     7921 ggaagaaaca aatttttagg tgtcattaca ggaaccatcg ctctaactat ttttatgagt
     7981 atcgcaaatt atttcgttat tacgcctctt tacttaatgt ttttagggct gaacgcaaca
     8041 cagatgctgg gcatgtcttt agcaaactat gtactgattg ggatcgttcc tttcaacttg
     8101 atcaaaggat tcatcgtgag tgctgtcttt ttagtgctgc atgcgcaact tcttccatgg
     8161 ttaagtcgaa aacaacatca gttcgaacaa cgtca
//
LOCUS       DAZKNO010000124         8155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000124 DAZKNO010000000
VERSION     DAZKNO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.126"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..123
                     /locus_tag="ACG8MY_002065"
     CDS             <1..123
                     /locus_tag="ACG8MY_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFQ0431239.1"
                     /translation="SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            299..1042
                     /locus_tag="ACG8MY_002066"
     CDS             299..1042
                     /locus_tag="ACG8MY_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431240.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1035..2615
                     /locus_tag="ACG8MY_002067"
     CDS             1035..2615
                     /locus_tag="ACG8MY_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0431241.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2866..3345)
                     /locus_tag="ACG8MY_002068"
     CDS             complement(2866..3345)
                     /locus_tag="ACG8MY_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0431242.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3361..4113)
                     /locus_tag="ACG8MY_002069"
     CDS             complement(3361..4113)
                     /locus_tag="ACG8MY_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0431243.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4230..4300
                     /locus_tag="ACG8MY_002070"
     tRNA            4230..4300
                     /locus_tag="ACG8MY_002070"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4262..4264,aa:Gly,seq:ccc)
     gene            4661..5386
                     /locus_tag="ACG8MY_002071"
     CDS             4661..5386
                     /locus_tag="ACG8MY_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0431244.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5641..5907
                     /locus_tag="ACG8MY_002072"
     CDS             5641..5907
                     /locus_tag="ACG8MY_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0431245.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            5904..6335
                     /gene="ruvX"
                     /locus_tag="ACG8MY_002073"
     CDS             5904..6335
                     /gene="ruvX"
                     /locus_tag="ACG8MY_002073"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0431246.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6360..6665
                     /locus_tag="ACG8MY_002074"
     CDS             6360..6665
                     /locus_tag="ACG8MY_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0431247.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6750..7895)
                     /locus_tag="ACG8MY_002075"
     CDS             complement(6750..7895)
                     /locus_tag="ACG8MY_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0431248.1"
                     /translation="MATIKQYEKKDGTKAWLFQTYLGINPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKQDTNETFQEIYELWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGKFRIKEITVKHVQKILNQWAKMTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRNQLEILFQYLESKRSTYRADYDKTLLRFLFFSGCRISEAL
                     ALNWSDIDFDSNTVTINKTLSQTKYGYKISSPKTKNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(7954..>8155)
                     /locus_tag="ACG8MY_002076"
     CDS             complement(7954..>8155)
                     /locus_tag="ACG8MY_002076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431249.1"
                     /translation="SDLKRATEFIKKFYSSKALQNVSREILEIIFSEQKDLLLGIQTV
                     RIIWEGVQYLPSETLTIDIETN"
BASE COUNT         2599 a         1373 c         1575 g         2608 t
ORIGIN      
        1 tctattagca gagtaaacga aatagtaaca tttgcaaatg gagaacaact agagatggga
       61 aagagaagta ttgataaact aaagaatttt atgcaagaaa agatagaaaa tgaatcattt
      121 taaagaaaac aaagactcat atcttacctg ttagaagagt gttttcctgt agaagagaac
      181 aacccaagta gcagcctagt cgtttctatc cttttttgtt atgcactctt tttgacattt
      241 actccaccaa taacgaaggg tacaataaaa agtaataaca aacaaaagga gtacagaaat
      301 gattgaattc atcaatgtaa gtaaaagtta cgggaaaaaa caagcattaa aagacttgtc
      361 tctctcgata agacaagggg aaatttttgg atttttagga cataatggtg ccggaaaatc
      421 aactacgatt aaaagtttag ttagtattat tgaaccatca tcgggaacga ttttagtaga
      481 tggaatgaaa ttgacggaaa atcgattgag tatcaaacaa aaaatcggtt atgtgcctga
      541 ttccccagat atttttttac aattgacggc tggagaatat tgggacttga tttcagctgc
      601 ctatgaatta gacacacaaa aaaaagaaaa gcgtttagca gaattgacag cgctttttga
      661 tatgtatagt catcaaaatg aaacaatcgc aagtttttca catggaatgc gtcaaaaaac
      721 gattttgatc ggcacgttgc tgccagatcc agatatttgg gtattggatg aacctttgca
      781 aggacttgat cctcaagcag catttgattt gaaagaaatg atgaaggctc atgccgcaaa
      841 aggaaaaaca gtcatttttt ctacacatgt attggatacc gcacaacagt tatgcgataa
      901 actggctatt ttgaagaaag gtgaattgat ttatcagggg gcagttagtg aattattgaa
      961 tggctcacct gatgaaacgc tagaaaagat ttatttgaaa atggctggaa ggcaagcgac
     1021 aggagatgga ccgtatgtat aagcgacaat tattagaatt aattcgagtt aatcttttat
     1081 atgttaatcc acagctcacg aataaagcaa gagagaaagg aaaaaaggga aagcgattaa
     1141 ctatctatct tttgtctcaa aatctttttt cagggctgat ttttttactg atttacggat
     1201 ttgctatgtt tgctatgaac tttgttaaat tgccaggttt ctttacttat tacgtggcac
     1261 tttttggtat gttaggttta actcaaagta tatcagtcat ttataatatc ttttttgaag
     1321 gaaatgacct gcaagtattt ttgcctctgc cttttggaca agaacaggtt ttttcagcaa
     1381 agattttggt agttgctttg accatcattc cttttgtttt tccgcttcta gtcatgttta
     1441 ttttaaccgg atggcgagct gatctaggga ttttgcttac tatcattatc tcaatccttt
     1501 tgttttctct atttttgatt attctttttt gtctggctag tttgattgtt tttggtttgg
     1561 ctcgaacagc agtgtttaaa aaacatagaa aaacagtttc tagtatcttg cttggcagct
     1621 cgatgctgat cgcatttgct ggaatcatgt ggatgaatca tcaaacgggg actattgaaa
     1681 atgagttaac agatcggcat ccgatttcga tattattgcc attctatgat gctgccagtc
     1741 agccactttc tcaacaaggt ttattgggtt ggggaatact agctatcacg gctctgggat
     1801 ttttccttgc tgtaaaacag ctgatagtac caaagcttac agagcaactg actgattcaa
     1861 acagtgagtt caaaataaaa agaacccata aaaagaacca aaatgtgcat cagcttttat
     1921 ttagttacaa tgctcaatta gtaaaagaac ctaatttgat tatgcaagtt ctttcttctt
     1981 ccttgttaac tccaatcatt tttattcttg cttttgccct aggaggcgaa ataaaattaa
     2041 ctgatttaga taataagtta atcggtgttg ttttcctggt agggatcgca ttggctttct
     2101 taacagttaa tcagacttct tttatcagca atcttatttc tttggatcaa gaaaattttc
     2161 tgtttatccg ttctttgcca atctctatga accaatattt aaaagaaaag ttcagattcg
     2221 gatggatatt gcagagtttt ttaactggtg ggatcgcatt attaagcgga ctgtcttttc
     2281 agctgccttt gttttttatc cctagcttgc tatttggttc tttattcggc tgctacttat
     2341 tgtcactacg ttatttcgct agagattatc ggttgctgct gctgaactgg accaatatta
     2401 accaattatt taacagaggt tcaggaagtt tgggtttagt cgcaactatg atgggttcat
     2461 tgatcatcag tattattctt atcgtcttat acggaatgtt ggctttgttt tatccatttt
     2521 ggttagtgaa tattcctgtg atcggtttag ttctaatact ttccgctcta tggatcgttt
     2581 attatcaacg aacgttttgg aaaaaatttg aataaagaag aggggccgtg acaaaaatca
     2641 tgtcgcagcc ccttttttac agatagaggg tgttcaaaag tcagtttctc agtattaagt
     2701 caattttatg taaaaatgtg agaagctatt tttacaaaac tttttttaat actcataatt
     2761 acttcttatt gctcctgcgg ttacttgtcg caatgtttgt gagatatttt gtcatcgctc
     2821 aaggctgaac gtctctttca caaccttttc tttgttacac ttcgtttatt taaaaagccg
     2881 tttttttcgt ttcatcatga ttgcgtcacg ctttactttg aaattacgga tgttatggtt
     2941 aaagacttct gataatacaa gacctaaagc gattgctcca gcaatcataa ttgcttggac
     3001 cgcataatga gccgcttctc gataggattc agtaacgaga tttcgaaccg cttgataagc
     3061 taaaccacca gggactagag gaaccatccc gggaatattg aaaatcgtta cgggcatttt
     3121 caaacgttta gagaaaagat cgctgatgaa tgctactcct aaagaaccaa gcaatgaacc
     3181 aaaagcagca gatccgccta tttgaacaca gatccagtag atcatccagc caaaagcacc
     3241 agaaagacca caacaaatca atgaacgcct aggtacattt gtaataacgg caaatgccgc
     3301 agtcgctaaa aaactaaaag aaaactgaat aaatatcgtc cccatacttg tgttcctttc
     3361 ctaataaaaa agctggaaaa taaaagcaat cgcaaaaccg atcatcgtag cagtcatcat
     3421 cgcttctgtt ccgcgagaaa ctcctgagac ataatgtcca gctagcaaat cacggatagc
     3481 gttcgtaatt tgaactccag gaactaaagg catcacacat ccgataatga ttagatcctg
     3541 attgcttcct aagccaaaac gtacgctgta gatagcggct atgccaatca ataaagaaga
     3601 taaaaattcc gataaaaatt tgattctaaa aaatttcaaa ctatatagat aagctgcgta
     3661 tcccaatcct ccaatgacta aagtcacagg taaatcggtc atctttccgc caaataacac
     3721 cataattgtt ccactgatca ctgccgcgct aacgatttgc aaccagacag gaaagaaaga
     3781 acggtctcgg tcgattgctt gcagttcgca gtaaagttct tctaatgtta gctctgaagc
     3841 aacatattta cgtgaaagat catttacttt tactactttt tctaaattaa tcgaacggtt
     3901 ggtaatttgt tccattcgga tggtcgaggt acgatcaaga ccaataaaga gaccggtttg
     3961 agtgacatag ctgaccaaac ggaaatttcc agaagctaaa gcaattcgac tcatcgtatc
     4021 ttctacccga tacatctccg cattgctttc catcatgatt ttcccagcga gtaaacaagt
     4081 gtccagcaac agatctaatt cttttcccgt catagcatct tctccaataa ttgattgatt
     4141 agttttattt tactacaaaa cataccagaa gcgtaaagaa taaaaaaact tgaggaagga
     4201 ataaaagagg cgtatactgt ttttgtattg cgggtgtagt ttagtggtaa aatcccagct
     4261 tcccaagctg atgtcgcggg ttcgattccc gtcacccgct taaaataggt tgtgaaaaag
     4321 ctgctctgca acaagtaata agaacagcca agcaaaaaca acttcttatg tttttaactt
     4381 ggatggactt attaccgcag atgcaagctt ttgcgcaccg tttaccaagc ggttgtgaaa
     4441 tcagtgtatt gtcttgagta tgaagaaaat ttttgaataa taataaatac cttacagata
     4501 gagtggtgac tgattagaga cgaggaatcc gtctcttttt tgtattttta agccatctta
     4561 aaacaggttt tattttttgc tgtttctatt tctttctagt atttatttat aggggaacat
     4621 atgttaagat ttagggagct gttagtaaag gagtaaagaa atgagcaaaa aaacaaccaa
     4681 ggcgatcgtg gggatcgtcg ctgctgtgat tgcaggggca ttcggtgttt ccaccacaca
     4741 aaaagattca gatatctttc agcagattac aactattttt gattccacac cacaagcttc
     4801 tcaagtagcg gatgtgccag aatatgatgg aaaacatcaa gaaatagaaa tcaatgggaa
     4861 tcagccggaa tttacaaaag aggatcttag cctttccaaa ggaacatggg aaagctattc
     4921 aaatattgat aaattgaaca gagtaggaca agccaatgct atgcttggaa aagatttgtt
     4981 ccctactgaa aagagagagc ctttgtatat tgacccgaca ggttggaaac aaaagaaatt
     5041 aagtgacggg caatggttat ataatcgcag ccatttaatt ggtttccagc tgactggtca
     5101 aaataacaac attaaaaact tgatgacagg gacacgttcg ttgaatgctc catacatgct
     5161 tgctcacgaa aatgatattg cggcatatat caaagaaaca aatcatcatg tacgctaccg
     5221 tgtcactcct cattttgagg ggaatgaact ggtagccagg ggagtgcaat tggaagcaga
     5281 aagcattgaa gacaaaaaaa ttgaattcaa tgtctttatc tacaatgttc aagatggcta
     5341 cacgatcaat tatgagacag gacaagcaac aaaaacaaaa aattagtagc agctattcga
     5401 attttttatg aatattatga aaaaattgtg taaaaacata cattcaaaga aatatagcgt
     5461 atactttatg aaaagagtac ttttgataaa gctacagaaa aaaggaaaaa aacatacaaa
     5521 tagtgtttct taatttattc ggaagtttgg aatatttgtt tgtagttttc cctttcttcg
     5581 agtaaaataa ggttgtagaa ggtaagctgg aattaccttg cgggaaagag gtgtagaatc
     5641 atgggtttta cagatgaaac cgtacgtttt ggttttgacg atagtcgcaa aaaagaagtg
     5701 agcgaaacat tagcgattgt ttatcgggct ttggaagaaa aaggctacaa tccaatcaat
     5761 cagattgtcg gctacttgct ttctggagac ccggcctaca ttcctcgtta tcgtgatgct
     5821 cgaaatttga ttcgccgtca cgaacgcgat gagatcatgg aagtaattgt caaggactac
     5881 ctctcaaatc atggagtgaa tttatgagag taatgggctt ggatgtcggt tctaagacag
     5941 taggtgttgc tatcagtgac ccctttggct ggacagctca aggagtagag atcattcgga
     6001 tcaatgaagc agaagaagag tttggctttg atcggttgaa ggaattagta gcacaatatg
     6061 aagtagaccg gtttgtagtt ggattaccta aaaatatgaa caattccatc ggaccaaggg
     6121 cagaagcttc catgtcatac ggagaaaaga tcaaagaact attctccttg ccagttgatt
     6181 atcaagatga acgattaacc actgttcagg cagaacgtat gctagtagag caggcaaata
     6241 cttcaagagc caaacgaaaa aaagtcatcg ataaattagc agctgtgatg atcttacaaa
     6301 attatttaga cggctcaggt aagcaattat tttaataata aaagaaaaga ggtaatctta
     6361 tgacagaaca taaccattcc cacgaccatg aaggacacga acacatcaca ttggtggatg
     6421 accaaggaaa tgaaacatta tatgaaatcc tactaacagt tgacggacaa gaagaatttg
     6481 gacgtaacta tgttctttta tatcctgctg gtgtttcaga agatgaagat gtagaattac
     6541 aagcttatgc atacatcgaa aacgaagatg gaacagaagg cgagttagaa caaatcgaaa
     6601 cagacgcaga atgggacatg attgaagaag tattcaatac attcatggct gaagaagaag
     6661 aatagacata aaatgatggc tgaacccttg gtagataagg gttcagcttt tttgtgtttt
     6721 gttttgaata cgagattgaa tacgttccgc tagaaattta ggtgttctgc aagcttttca
     6781 acggtttttt ctttggtttc tttagttaga tgagcataag tatccattgt taatttgatt
     6841 gatgagtgtc taaggcgttc ttgaacttcc ttcatagaaa ctcctgactc taacaacata
     6901 ctagcatgag tgtgtctggt tacatggttt cctagaaatg gaataccaga attcttgcag
     6961 attgtgatca tacgagaata aatagcgtgt ttagtaatga tttccttgta gattccacaa
     7021 aaaatcattg ttggatcagt aataccaagg gttaacatat atcttttttg attgatttgc
     7081 catttcttta gccaattaat ggttttattg tctaaagata gagaaccata gctatttttc
     7141 gtttttggac ttgaaatttt gtatccatat tttgtttgac ttaaggtttt gtttatagta
     7201 acggtgtttg aatcaaagtc aatatctgac cagttcaaag ctaaagcctc actaattcga
     7261 caaccactaa aaaacagaaa gcgaagtagc gttttatcgt aatctgctct ataggtactt
     7321 ctttttgatt ccaagtattg gaagagaatt tctagttgat tccgtgaata aattttgaca
     7381 gtatctttct ttttttcaac atttctattg ggcatgatta caagttcaca ggggttttta
     7441 gtaattagcc ccaaagtcat tgcgtatttg aaaatacggc ttacagtaga atgtaggact
     7501 ttatattgat ctgtcatttt cgcccattga ttcaatattt tctgaacgtg ttttacagtg
     7561 atttctttga tcctgaattt accatatttt ggaagaatcc atttattgaa cttgatctca
     7621 atttttaatt gagtgacttc ctttacagtg gttttatagc tttcatacca aagttcatag
     7681 atttcttgaa aagtctcgtt cgtatcctgt ttaagaccgt tctcttcaaa gtctacaagt
     7741 agtctattaa gctcgatttg agcttctttt tttgttttaa aatttctacg tgtagtacgg
     7801 acttctttac cagttaatgg attgattcca aggtaagttt gaaacaacca tgctttggtt
     7861 ccgtctttct tttcatattg tttaattgtt gccattgttt ttcctcctat cagttgcgtg
     7921 gcgtgctttt gataggaaat aaatctgatt taactaattt gtttctatat cgatagttaa
     7981 tgtttcacta ggtaagtatt gaactccttc ccagattatt cttactgtct gaatcccaag
     8041 tagtaagtct ttttgttctg aaaagataat ttctaatatt tctcttgaaa cattttgaag
     8101 tgcttttgaa gaataaaatt tttttatgaa ttctgttgct ctttttaaat ctgaa
//
LOCUS       DAZKNO010000125         7985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000125 DAZKNO010000000
VERSION     DAZKNO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.127"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..215
                     /locus_tag="ACG8MY_002077"
                     /pseudo
     CDS             85..215
                     /locus_tag="ACG8MY_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031600262.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(280..1056)
                     /locus_tag="ACG8MY_002078"
     CDS             complement(280..1056)
                     /locus_tag="ACG8MY_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0431250.1"
                     /translation="MIGFIYFIVIVLANTVGAVSGMGGGVLIKPIFDFIGAHSVAAIS
                     FYSTVAVFTMSLVSTARQLASGRKIHWQIVLWVSGGAVLGGILGNVAFDHLLLLFQNE
                     DEVQMIQIFLTVVTLLFAFFYTKYDWPGFQLKNNFWYCFCGLVLGFLASLLGIGGGPI
                     NVSLLMLMFALPIKEATVYSICTIFFSQLAKIVTIAGTTGFGLYDLKILWFVIPAAIV
                     GGLLGAKVSTVLSPQKVTLVFQTVIFLVLIINLYNGYQLL"
     gene            complement(1053..2165)
                     /locus_tag="ACG8MY_002079"
     CDS             complement(1053..2165)
                     /locus_tag="ACG8MY_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HFQ0431251.1"
                     /translation="MKHISVLIKPASSLCNLRCSYCFYANVSSLREVRSFGKMKEEVT
                     EKMIENIYADLEDGDQLTLAFQGGEPTMAGLNYFRTVIRLVAQQQKQVQVHYAIQTNG
                     TMINEKWCAFLKEHDFLVGLSIDGHPLYHDLHRVDPKGRGTFQRVLQTKQLFDYYEID
                     YNVLCVLTNPLAKEAKKVFRFLKEQQIDFVQFIPCLDDLDAKKRNSYALTPKRFAGFY
                     HQLLQLWLQELAEGHYISIKLFDDLLNLLIKRQVTACGILGNCQVQYVIEADGSVYPC
                     DFYVLDEYRMGYIQEQTLRQLFEQDIAKTFLCGRPEMPKKCLSCPFQKMCRGGCKRMK
                     DAIYVDNTGYCGYQQLLQEFVPQINRILGLLQGVSV"
     gene            complement(2246..3016)
                     /locus_tag="ACG8MY_002080"
     CDS             complement(2246..3016)
                     /locus_tag="ACG8MY_002080"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0431252.1"
                     /translation="MKSQQFFFKYVNIDASNLKGELIIKPKYLEIKQNLKQQIVTGQF
                     HNGDRFYSESEIIKKYNVSSITAIRALKELVAEGYIVRYQGIGTFVSRARKEKIVKFS
                     DIEIFSFDNDKTHVLSIKRENKKQILDTLKLSTDSYYYQIERIREANEIPYIYHKSYL
                     PEQYINSNYPEINYYNSIYKRLKSDYNIDLNEENFRETNEILFPAPNSVCKQLKINES
                     EPVVFQTKLSCLSENNKPIEYVESYKKWNFYKIQLLSN"
     gene            3070..4848
                     /locus_tag="ACG8MY_002081"
     CDS             3070..4848
                     /locus_tag="ACG8MY_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase family protein"
                     /protein_id="HFQ0431253.1"
                     /translation="MTTFEIKEEFLLKGKTFKILSGAIHYFRIPPCDWEHSLYNLKAL
                     GFNTVETYVPWNLHEPQKGEFHFEGILDLERFLTIAQDLGLYAIVRPSPYICAEWEFG
                     GFPSWLLREPIHIRRNEIAYLEHVADYYDVLMKRIVPHQLNNGGNILMIQIENEYGSF
                     GEEKEYLRAIRDLMIKRGVTVPFFTSDGPWRATLRAGSMIEDDILVTGNFGSKAKDNF
                     NSMKQFFKEYDKNWPLMCMEFWDGWFNRWKEPIIQRDPQELAEAVKEVLEQGSINLYM
                     FHGGTNFGFMNGCSARGVIDLPQITSYDYGAPLDEQGNPTEKYYALRKMIHDNYPEIK
                     QLDPVIKPTIEKKKISLTNKVSLFATLDTISQPVQSKYPKTMEELGQNTGYLLYRTMI
                     ERDAEEERLRVIDGRDRSQLFLNQELQATQYQTEIGEDIQVSMTEEINQVDILIENMG
                     RVNYGHKLFAETQKKGIRTGVMADLHFITNWQQYSLPLENCKTVDYSKGWQPQHPSFY
                     RYEFLLEDIGDCFIDLRKFGKGIVFVNQTNIGRFWEVGPTLSLYISSGFLKVGKNEIV
                     IFETEGTYEPEINLLKAPLYKEMKEG"
     gene            4852..5331
                     /locus_tag="ACG8MY_002082"
     CDS             4852..5331
                     /locus_tag="ACG8MY_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0431254.1"
                     /translation="MSIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVAQNDIEK
                     SGLKLATPAGVKLSILPIEKAAQNILAGKYDSQRLLIIARKPDRLLKLVELGVPIKEI
                     NVGNMSQTSETRSITKSINVVDQDVETFNQLNEKGVHLIAQMVPSDKADDFMSLLNK"
     gene            5354..5761
                     /locus_tag="ACG8MY_002083"
     CDS             5354..5761
                     /locus_tag="ACG8MY_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0431255.1"
                     /translation="MAGHLVLISHGRFCEELKRSVDMIMGVQKDIYTVGLLPEESAKD
                     FRVNFEKTINGLKKVTVLADLMGGTPCNVVSQLILEGKEIELYAGMNMPMVIGFVNNQ
                     FLGEDSSLVNKGRENIIHVNEYLEVNPIDLEDE"
     gene            6012..6329
                     /locus_tag="ACG8MY_002084"
     CDS             6012..6329
                     /locus_tag="ACG8MY_002084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431256.1"
                     /translation="MVFEDQYLQIKNSENIEACIEISNSQESNIFVSPENGPVKPNFN
                     YLTYDRFSQNTVFDGYKLEQSSPAIHSGKKVIDKNGYNLGTDFFGIKLDGILDIGAVK
                     SSK"
     gene            6351..7163
                     /locus_tag="ACG8MY_002085"
     CDS             6351..7163
                     /locus_tag="ACG8MY_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0431257.1"
                     /translation="MNDISFFQIFSLICLAILGGIDPYLSGLQLSKPAISGFLAGIIM
                     GDINTGLLVGATLQLMVLGVGTFGGSSIPDFGTGAIIGTALGVVSGKGIEFAIGISVP
                     VGLLLVQLDILARFCGVYFLHRVDKHIEEENFHKISLEAWLSLVPIALSRAIPVGLCL
                     IFGNEVVNTVLNYAPDWLMGGLKLAGAILPVVGIAILLHYLPIRNFFGFLIIGYILAA
                     YLNIPMMGISFAGLAAAIIHYKKLQDKRLLESKLDTINHIEVVNGEVDDDEI"
     gene            7150..7965
                     /locus_tag="ACG8MY_002086"
     CDS             7150..7965
                     /locus_tag="ACG8MY_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0431258.1"
                     /translation="MMKSNVSKLSSKDLRKVSLRYMFGAQLGWNYERMMNVAYVRAVY
                     PALKKLYKDEKELKSVLQSEMQFYNTSPFLSAFIIGIDLALQVEDGSKSKDAVAAIKT
                     SMMGPFAAVGDSLFGAVIPTIFGSLAAYMGLEGNPLGVLLWLLVSLIILFLRYFELPI
                     AFREGKKLVSSVGGLLKDLTESATLLGVVVIGGLVPSVVKVGVPFKLVIGDKKLSFQT
                     EMLDPIMPAIVPVVLVLLAYWLLSKKSLNSTKVIWIFLVLSILMYSLNILAVL"
BASE COUNT         2655 a         1197 c         1582 g         2551 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgaccca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcctatttt gttagtagat gtctggaaac ggtactttgc acatggtgaa
      121 caggcagtgc tattagaaaa atcatgcata ttactaatgc aattgagagt atttattcta
      181 gtttcagaaa agtcacaaaa aagagcgctt cttaatgaga aagtacttct aaagttacta
      241 attaatcgtt ttctataagt ggttgataat actcgttttt tatagtaatt gatagccgtt
      301 ataaagattg atgattagca ccaagaagat gactgtctgg aagaccagcg tcactttttg
      361 tggtgagagg acagtgctga cttttgcccc taacaaaccg ccaacaatcg cggctggaat
      421 cacaaaccac aaaatcttta agtcatagag gccaaatcca gtggtcccag caatcgtcac
      481 gattttcgcc aattgagaga aaaaaatcgt acaaatcgaa tagaccgtgg cttctttgat
      541 gggtaaagca aacatcagca tcagcaagga cacattgatc ggtccaccgc caatgcccag
      601 cagactcgct agaaaaccaa ggactaggcc acagaagcaa taccagaagt tatttttcag
      661 ttggaatccg ggccagtcgt atttggtata aaagaaagca aacagcagtg tcacaacggt
      721 caaaaagatc tgaatcattt gtacctcatc ttcattctga aataaaagca gcaggtgatc
      781 aaaagcgaca ttgcccaaga tgcctcctaa gacggcgccg cctgaaaccc aaaggacgat
      841 ttgccaatgg attttacgtc cactagccaa ctgtcttgcg gtcgaaacca gcgacatggt
      901 aaacacggct acggtagaat aaaacgagat ggctgcaaca gaatgggcac cgataaaatc
      961 aaaaatgggt ttgatcagaa caccgccccc catgcctgaa acggcaccaa ccgtattcgc
     1021 caacacgatc actataaaat aaatgaaccc aatcacacac ttaccccctg taacaaacct
     1081 aaaatccgat taatctgcgg tacaaattcc tgcaaaagtt gctgataacc acaatagcct
     1141 gtgttatcta catagatggc atctttcatc cgtttgcagc cgccacgaca cattttttga
     1201 aacgggcaac tcagacattt ctttggcatt tcggggcgtc cacacaaaaa cgtttttgca
     1261 atgtcctgtt caaataactg acgtaaagtc tgttcttgaa tataccccat gcgatactca
     1321 tccagcacat aaaaatcaca aggatacaca ctgccatctg cttcaatgac atattgtacc
     1381 tgacagtttc ctaaaatccc gcaggcagtg acttgtcgct tgatcaatag attcaaaaga
     1441 tcatcaaaaa gcttgatact tatataatgc ccctccgcta attcttgcag ccataactgc
     1501 aaaagctggt ggtaaaatcc tgcaaaacgt tttggtgtga gagcataact atttcttttt
     1561 tttgcatcta gatcatctaa acaaggaata aactggacaa aatcaatttg ttgctctttt
     1621 agaaagcgaa agactttctt agcctctttt gccaacggat tggtcaagac acaaagtaca
     1681 ttgtagtcga tctcatagta gtcaaacagc tgttttgttt gcagcactcg ctgaaaagtt
     1741 cctcttcctt tcggatcgac ccgatgcaga tcatggtaga gcggatggcc atcgatcgag
     1801 agaccgacca aaaaatcatg ttctttcaaa aaagcacacc atttttcatt gatcatggtc
     1861 ccattcgttt gaatcgcata atggacctgt acctgctttt gttgttgcgc tactaaccgg
     1921 atcactgtac gaaaataatt gaggcccgcc atcgttggct ctccaccttg aaacgctaat
     1981 gttaattgat cgccgtcttc taagtctgca taaatatttt cgatcatttt ctctgtcact
     2041 tcttctttca ttttgccaaa cgagcggact tctcgcaagg aactgacatt ggcataaaag
     2101 cagtagctgc agcgaagatt gcagagggaa gatgcgggtt tgatcaatac tgaaatatgt
     2161 ttcatagtaa gaacctcatt ttttaacata gtttaattat gaaaaattgc tataacgaac
     2221 aaagtaaaat caaattcagt agtagttaat tagataggag ttgaatctta tagaaattcc
     2281 attttttata actttcaaca tattctatag gtttattatt ttcagataag caggataatt
     2341 tggtttgaaa aacgacaggc tcagattcat ttatttttag ttgtttacat acactattag
     2401 gagcaggaaa taaaatttcg tttgtttccc taaagttttc ttcgtttaaa tcaatgttgt
     2461 agtctgattt taaacgtttg taaatagaat tgtaataatt aatttctgga taattagaat
     2521 ttatatattg ttctggcaaa taagatttat gatagatata aggtatttca ttagcttctc
     2581 taattcgttc gatttgataa taatatgaat cagttgataa ttttaatgta tctaatattt
     2641 gttttttatt ttcgcgttta atcgaaagaa cgtgtgtttt atcattgtca aatgaaaata
     2701 tttctatatc agaaaatttt actatttttt cttttcttgc acgtgaaacg aaagttccta
     2761 ttccttgata acgtacgata tacccctcgg caactaattc ttttaacgca cgaattgctg
     2821 ttatagaact aacattatat tttttgataa tttctgattc tgaatagaat cggtcgccat
     2881 tatgaaattg accggttaca atttgttgtt ttagattctg tttgatttct agatatttcg
     2941 gttttattat aagctccccc tttaaattac tcgcatctat attaacatac ttaaaaaaaa
     3001 attgttgact tttaatacaa aagcattaat atactaagta tattaacaca ttgaggtgaa
     3061 aagtaacaga tgactacatt tgaaataaag gaagaatttt tattaaaggg aaaaacattt
     3121 aaaatactat ctggtgcaat tcattatttt cggattcccc catgtgattg ggaacattct
     3181 ttgtacaatt taaaagcact aggatttaat acagtagaga catatgttcc ttggaattta
     3241 catgagcctc aaaaaggaga gtttcatttt gaagggatat tggatttaga gagatttttg
     3301 acaatcgctc aagacttagg attatatgct attgtgcggc catcgcctta tatttgtgct
     3361 gaatgggaat ttggagggtt tccgtcatgg ctactccgtg aaccgattca tatccgaagg
     3421 aatgaaatcg cttatttgga gcatgtagcg gattattatg atgttttgat gaaaaggatt
     3481 gttccccatc aacttaataa tgggggaaat atattgatga ttcaaattga aaatgagtat
     3541 ggatcttttg gggaagaaaa ggaatatttg cgtgcgataa gagatttgat gattaagcga
     3601 ggagtgacag ttcctttttt cacttcagat ggaccgtgga gggctacttt acgagcggga
     3661 agcatgattg aagatgatat tttggttact ggtaattttg gttctaaagc aaaagacaac
     3721 ttcaactcta tgaagcagtt ttttaaagaa tatgataaaa attggccact gatgtgtatg
     3781 gaattttggg atggttggtt taatcgttgg aaagaaccaa ttattcaacg agatccacag
     3841 gaactcgctg aagcggtaaa agaggtatta gaacaaggaa gcattaattt atatatgttt
     3901 catggaggaa cgaattttgg gtttatgaat ggttgttcag caagaggagt gattgattta
     3961 ccgcaaatta cttcctatga ttatggtgca ccattagatg agcaggggaa tccaacagaa
     4021 aaatattatg cattgcggaa aatgattcat gataattatc cagagattaa gcagctagat
     4081 cctgtcatta aacccacgat tgaaaagaaa aagatttctt tgacgaataa ggtaagtctt
     4141 ttcgcgactt tagataccat tagtcaaccg gttcaatcaa agtatccaaa gacaatggaa
     4201 gagttgggac aaaatacagg ttatctactt taccgaacaa tgatagagag ggatgcagag
     4261 gaagaacgat tacgggtaat tgatggcaga gatcgcagcc aattattttt gaatcaagaa
     4321 ttacaagcta cccaatatca aacagaaata ggtgaagata tacaagttag tatgacagaa
     4381 gaaatcaatc aagtggatat tttgattgaa aatatgggac gtgtcaatta tggacataaa
     4441 ctctttgcgg aaacgcaaaa aaaaggaatt agaacgggag taatggcaga cttacatttt
     4501 attacaaatt ggcaacagta ttcattgcct ttggaaaatt gtaaaactgt ggattattca
     4561 aaaggatggc agccccaaca cccaagtttt tatcgatatg agtttctttt agaggatatc
     4621 ggtgattgtt ttattgattt aaggaaattt ggtaaaggaa ttgtctttgt taatcagacc
     4681 aatataggtc gtttttggga agtaggtcca acgctttcac tgtacatctc tagcggcttt
     4741 ctaaaagttg gtaagaatga aattgttatt tttgagacag aaggtaccta tgaaccagaa
     4801 atcaatttat taaaagcacc attatataaa gaaatgaaag agggatgaac aatgagtatt
     4861 attggagtaa gaattgatgg acgtttaatt cacggacagg tagctaattt atggacaacc
     4921 aaattgaata tttctcgaat tatggttgtg gatgatgaag tggctcaaaa tgatattgaa
     4981 aaaagcggat tgaaattggc taccccagca ggggtgaaac taagtatttt gccgattgaa
     5041 aaagcggcgc aaaacatttt agcaggaaaa tatgattcac aacgtttact gattattgca
     5101 cgaaaacctg atcgattatt aaaattggta gaattgggcg ttccaataaa agagattaat
     5161 gttggaaata tgtcacaaac ttctgagact cgatcaatta cgaaatcaat taatgttgtc
     5221 gatcaagatg tagaaacgtt caatcaatta aatgaaaaag gtgtccattt aattgcgcaa
     5281 atggttccaa gtgacaaagc agatgacttt atgtctctgt taaataaata ataatatacg
     5341 aggaggtgaa aagatggcgg ggcatttagt tttaattagt catggacgat tttgtgaaga
     5401 gttaaagaga agtgttgata tgatcatggg agtacaaaaa gatatttata ctgtagggtt
     5461 attacctgaa gagtcagcaa aagattttcg agtaaatttc gaaaaaacaa ttaatggttt
     5521 gaaaaaagtt acagttctgg ctgatctaat gggaggcact ccttgcaatg ttgtttctca
     5581 attaatttta gaaggaaaag aaatagaact atatgcagga atgaatatgc caatggttat
     5641 aggctttgtt aataatcaat ttttaggaga agatagttct ttggtaaata aaggaagaga
     5701 aaatattata catgtaaatg aatatttgga agtaaatcca atagatttag aagatgaatg
     5761 aatgtggagg aatttatgaa tataaaaaaa tccaaatata ttttattgca acttgctgtt
     5821 tgatcagttt atctatgtta acaaaagtcg ctcatgctga aagtaacccc attggtaatt
     5881 gttactatgt tagttcgcaa agagggagtg acactaattc aggattatct atatctgagt
     5941 cttttaaaag tttaaataaa ataaatgaac ttaaataaaa tccaggggat aaagttttat
     6001 tagaaaatgg attggttttt gaagatcagt atttacaaat aaaaaattca gaaaatatag
     6061 aggcgtgtat tgaaatcagt aatagccaag agtcgaatat atttgtttct cctgaaaatg
     6121 gtcccgtaaa acctaatttt aactatttga catatgatcg attcagtcag aatacagtgt
     6181 ttgatggata taaattggaa caatcatctc ctgctattca tagtgggaag aaggttatcg
     6241 ataaaaatgg ttataattta ggaactgatt tttttggtat taagttagat ggcatactag
     6301 atattggggc agtgaaaagt tcaaaataat ataaaggagt tagtttgatc atgaatgata
     6361 tttctttttt tcagatattt tctttaatat gtttagctat actaggaggt atagatcctt
     6421 atctgtcagg attacaatta agtaaaccgg caatttctgg ttttctagca ggaattataa
     6481 tgggagatat taatacaggc ttattagtag gagcaacatt acaactgatg gtcttaggtg
     6541 taggaacatt tggtggctca tcaattcctg attttggcac aggggcaatc attggtacag
     6601 cattaggtgt tgttagtgga aaaggaattg aatttgcaat aggtatttct gttcctgtag
     6661 gcttattatt agttcaatta gatattttag ctcgtttttg tggggtttat tttttacatc
     6721 gtgtggataa acatatagaa gaagaaaatt ttcataagat tagtttagaa gcatggttaa
     6781 gtttagttcc tattgcatta tctcgtgcaa taccagtagg tttatgttta atttttggta
     6841 atgaagtagt aaatacagta ttaaattatg caccggattg gttaatggga ggattaaaat
     6901 tggcaggagc tatattacca gttgttggga ttgccatttt actacattat ttgccaatta
     6961 ggaatttttt cggtttttta attattgggt atattttagc cgcttattta aatattccaa
     7021 tgatgggtat ttcttttgca ggattggcag ctgctattat tcactataaa aaattacaag
     7081 ataaacgttt attagaatca aaactagata ctatcaatca tatagaagta gttaacggag
     7141 aggttgatga tgatgaaatc taatgttagc aaattaagta gcaaagatct gagaaaagtt
     7201 agtttaagat atatgtttgg agctcaacta ggttggaact acgaacgaat gatgaatgtg
     7261 gcatatgttc gagctgtata tccagctttg aaaaagttat acaaagatga aaaagaatta
     7321 aaaagtgtgt tgcaatcaga aatgcaattc tataatacca gtcctttttt aagtgcattt
     7381 attattggaa ttgatttagc attgcaagta gaagatggaa gcaaatcgaa agatgctgtt
     7441 gcagcaatta aaacgagtat gatgggacct tttgcagctg taggcgattc tttatttggg
     7501 gcagtgattc caacaatttt tggatctcta gcagcttata tgggattaga aggaaatcca
     7561 ttaggtgtac tactttggtt attagtatct ctcattatac tatttttgag atattttgag
     7621 ttaccaattg ctttccgaga aggtaaaaaa ctggtatcca gtgtaggtgg attgttgaag
     7681 gatttaactg aatcagcaac tttacttggc gtagtagtaa taggaggctt ggttcctagt
     7741 gttgtaaagg ttggtgttcc attcaagtta gttattggtg ataagaaact atctttccaa
     7801 acagaaatgt tagaccctat tatgccagct atagtacctg ttgttttagt tttattggca
     7861 tattggttat taagcaaaaa atctctcaat tcaaccaaag ttatttggat atttttagtt
     7921 ttgtcaatac taatgtattc attaaatatt ttagcagttt tgtaatagat gtgggggcgt
     7981 gatca
//
LOCUS       DAZKNO010000126         7633 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000126 DAZKNO010000000
VERSION     DAZKNO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.128"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      26..192
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            340..897
                     /locus_tag="ACG8MY_002087"
                     /pseudo
     CDS             340..897
                     /locus_tag="ACG8MY_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            955..2994
                     /locus_tag="ACG8MY_002088"
     CDS             955..2994
                     /locus_tag="ACG8MY_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0431259.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3103..4254)
                     /locus_tag="ACG8MY_002089"
     CDS             complement(3103..4254)
                     /locus_tag="ACG8MY_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0431260.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4408..4938)
                     /locus_tag="ACG8MY_002090"
     CDS             complement(4408..4938)
                     /locus_tag="ACG8MY_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0431261.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5037..5387)
                     /locus_tag="ACG8MY_002091"
     CDS             complement(5037..5387)
                     /locus_tag="ACG8MY_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0431262.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5544..7073)
                     /gene="ahpF"
                     /locus_tag="ACG8MY_002092"
     CDS             complement(5544..7073)
                     /gene="ahpF"
                     /locus_tag="ACG8MY_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0431263.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7083..>7633)
                     /gene="ahpC"
                     /locus_tag="ACG8MY_002093"
     CDS             complement(7083..>7633)
                     /gene="ahpC"
                     /locus_tag="ACG8MY_002093"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0431264.1"
                     /translation="KKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFTFVCPT
                     ELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSHQISRI
                     FDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRTHPGEV
                     CPAKWKESGETLKPSFDLVGKI"
BASE COUNT         2603 a         1474 c         1406 g         2150 t
ORIGIN      
        1 tcatgaactc gtaaatagaa tatagtaata accttgttag gtgaggctcc tatacaaaca
       61 taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac tgttggatta
      121 aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa aactcggacg
      181 atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt tctttatatt
      241 tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt ggattgctgt
      301 gtgaggagga gatgcggtga aaaaagatcg attggtggag gtcgctcgga ttcacttctg
      361 gttgcacaat acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca
      421 tatgtcgata ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga
      481 gttgatttat taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca
      541 agtatcacat ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag
      601 atcgatgcag aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg
      661 atggatggta atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc
      721 gggaggatca gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta
      781 acttcaatat tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac
      841 cttccagaaa gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat
      901 aaacaggtat aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa
      961 agaagtgaca agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta
     1021 atagcagctg cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta
     1081 gaagctggag aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac
     1141 aaagctgcag aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag
     1201 atcttagata aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg
     1261 aatctaaaag tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg
     1321 aatacctcat taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat
     1381 ggtcaaacca cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac
     1441 aaagtaagcc tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa
     1501 ccattagcaa caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat
     1561 ggaggcgaaa aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat
     1621 gctatcaatc aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa
     1681 atcattgatg gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc
     1741 agatattatc ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca
     1801 gcagaagata ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt
     1861 ttggagatgg cttttgataa ggatcttcgt gggacaacag gtgggaaatt aagcattaca
     1921 gatgcagacg gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat
     1981 atcaaattga caatcgatgc gaaggcacaa aaaatagctt ttgacagtct aggaggaaaa
     2041 gctgcatcaa ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct
     2101 ccaagctatg atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat
     2161 gaagaaaatc ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct
     2221 acgtttaaaa tgattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa
     2281 gtgttgacga tcaacgggct taaatggcaa aaagacagtt cttggggatc ttttaaaaca
     2341 acgcgtgtca gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat
     2401 aatatatatg cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg tacaggctta
     2461 gataaattca tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt
     2521 tctaatgaag atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc
     2581 gaacttctaa ttactcctat ccagcaagca gcaatgtatt ctgtttttac caacaatggc
     2641 acacttgtct atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc
     2701 agcgaaacag cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat
     2761 ggtacagcac attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg
     2821 gaaatcaaag aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat
     2881 cttgataacc aaggttatat gatggtcagc atgttggaaa ataaagaaga tgatgattca
     2941 gcaaccaaac gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt
     3001 gctttttaag tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa
     3061 cggttttcac gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt
     3121 ttttgtaaaa tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac
     3181 taaaggtgaa tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat
     3241 caaagccatt tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga
     3301 aaaaccggca atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag
     3361 cgtcaatata gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc
     3421 tacactgaca aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta
     3481 tacttcttct ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac
     3541 agagctaagc ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac
     3601 gatgataata gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat
     3661 ccatttgact aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg
     3721 taaagaagca ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa
     3781 aacgactaaa atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt
     3841 cactacgttt agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat
     3901 aatcccaaag aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc
     3961 aaaaacaacc gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt
     4021 tttcaaaaga gataaatcac tttccagacc agctaaaaac attaataaga tcacaccaat
     4081 ctccgaaaag tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag
     4141 caaaacacca acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg
     4201 tgagccaatc gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat
     4261 cattcctttg tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg
     4321 aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat
     4381 acggtctatt ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa
     4441 attgataatc tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag
     4501 catcaaactt ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact
     4561 actataaaca ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct
     4621 gttgatgata acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa
     4681 agcatagacg atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca
     4741 agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac
     4801 aagataggcg gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat
     4861 cgcaatccca gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg
     4921 tttaatcgtc tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca
     4981 ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa
     5041 cttctgagga tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa
     5101 taacgaattt ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca
     5161 cctgtaatca aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa
     5221 gtcacttgtt tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa
     5281 atgatttcat ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata
     5341 ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt
     5401 tctttttttc aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt
     5461 attcaaaaaa acaaaaagga ttgtgacaaa attcttgtca ccatcctttt ttccgctctc
     5521 tgacttttta caatcttcat agctcaagtg cgcatcagat aatcgaatgc tcctaaagct
     5581 gcagtagctc ctgaacccat tgaaacaatg atttgtttgt atggactatc tgtacaatca
     5641 ccagctgcga aaacgccggg gatattcgtt gcgccatttt tctcggtcac tatttcattt
     5701 cttggattca tttcgatcgt tctatctagc caatccgtat tagggagtaa gccgactaga
     5761 atgaatactc ctgaaacagc tagtgtatgc atctccatcg tacggcgatc gatataggag
     5821 agagattcta cctgattcgt accgctgatt tctttcgtcg ctgcatgttt gataacagtg
     5881 acattcgcaa gagagactag tttatcctgt aagatttgat ctgcttttaa ttctggtaag
     5941 aattctagaa catatacatg ttttgctaac ccagccaaat cgattgctgc ttcaacacca
     6001 gaattaccac caccgataac agcaatctct tttcctttaa ataaagggcc gtcacagtgt
     6061 gggcaataag caattccttt atttttaaac tctttttcgc ctggtacatt gatagaacgc
     6121 cagtgcgcac ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc gttagctaag
     6181 ccaatattaa taaaatgttg ctctttattt aaggaaacgg cctgttgatt catcataatg
     6241 tcgatagggt aactacgcac atgactttct acttgctgca tcaattttgg accttctgtg
     6301 taagaggtac caatcatatt ttcaatccct agtgtttcaa cgacttgtcc gccgaaagaa
     6361 tcagcgacaa gtcctgtacg gattccttta cgtgctgcat agatagctgc actactagct
     6421 gctggtccac cgccaatcac taatacatca taaggttctt tttcaaacaa ctcttcttca
     6481 atcaaagggc cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc gctactaaat
     6541 tcttctccat ttaaaaacac agttggcact gccatgattt ttttatcatc gatttctttt
     6601 tggaacatcc caccttcaat catcgtatga gtaatatttg gatttaatac agctaatata
     6661 ttcaatgcct gtacaacgtc ggggcaatta tgacaagtca agctaacata actgtcaaaa
     6721 tgcaatgttt gtgttatttt tttgatttta agactcaaag attcactgat tttcggtgcc
     6781 cgtccactaa tttgtaacat agccagtaaa aaagaggaaa attcatgacc gagggggata
     6841 ccagcaaaaa cgattccact ttctttgcca actggattga tagaaaaact aggtgtgcgc
     6901 atcgaatctc ttttttccca agagatttta ggagacattt ctgctatttc cttcgtaaat
     6961 tcagctagtt tttgactatt ttcactatta tccaaactaa cagaaaaaac aataggagat
     7021 tcaatcagtg ctaaatattg ttcgagattt tgtttagttt ctttgtctaa catatccacc
     7081 acctaaatct ttccaacaag atcaaaactt ggtttcaatg tttcgccact ttctttccat
     7141 tttgccggac aaacttcacc tgggtgggta cgtacatatt gagcagcgcg aatcttatca
     7201 atcagtgcac tagcgtcacg accgattcca tctgcattga tttccatagc ttgaacgatt
     7261 ccgtctggat caataataaa tgttcctcgc tgagctaatc cttgctcttc atccaaaaca
     7321 tcaaaaatac gagaaatctg atgagacgga tcaccgatca tcacgtattc gattttaccg
     7381 atagcatctg atgtatcatg ccatgctttg tgagtaaagt gggtatcggt agagcaagaa
     7441 aagacttcaa cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc ttctaattca
     7501 gtaggacaaa caaaagtaaa atcagctgga tagaagcaaa gaatactcca gttccccata
     7561 agatcttttt cactgacttc taaaaattct ccttgatgat aagcatgtgc attaaattct
     7621 tcgatttttt tcc
//
LOCUS       DAZKNO010000127         7590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000127 DAZKNO010000000
VERSION     DAZKNO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.129"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..418)
                     /locus_tag="ACG8MY_002094"
     CDS             complement(128..418)
                     /locus_tag="ACG8MY_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431265.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(536..796)
                     /locus_tag="ACG8MY_002095"
     CDS             complement(536..796)
                     /locus_tag="ACG8MY_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431266.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(933..1346)
                     /locus_tag="ACG8MY_002096"
     CDS             complement(933..1346)
                     /locus_tag="ACG8MY_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0431267.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1802..2287)
                     /locus_tag="ACG8MY_002097"
     CDS             complement(1802..2287)
                     /locus_tag="ACG8MY_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431268.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2426..2641)
                     /locus_tag="ACG8MY_002098"
     CDS             complement(2426..2641)
                     /locus_tag="ACG8MY_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0431269.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2865..3665
                     /locus_tag="ACG8MY_002099"
     CDS             2865..3665
                     /locus_tag="ACG8MY_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0431270.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3684..5552
                     /locus_tag="ACG8MY_002100"
     CDS             3684..5552
                     /locus_tag="ACG8MY_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0431271.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5545..6036
                     /locus_tag="ACG8MY_002101"
     CDS             5545..6036
                     /locus_tag="ACG8MY_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0431272.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6024..6578
                     /locus_tag="ACG8MY_002102"
     CDS             6024..6578
                     /locus_tag="ACG8MY_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0431273.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6596..>7590
                     /locus_tag="ACG8MY_002103"
     CDS             6596..>7590
                     /locus_tag="ACG8MY_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0431274.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2705 a         1212 c         1442 g         2231 t
ORIGIN      
        1 tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata cttcaaaaaa
       61 gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt taatacattt
      121 taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta agagagatca
      181 taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca tcccatattt
      241 cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg atccagcacc
      301 atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag ggaagtaaaa
      361 acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc tcccccacat
      421 tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt gtatgaataa
      481 cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg gaagtttatt
      541 tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat aagccgattt
      601 cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc ttatggataa
      661 aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg aataagtaaa
      721 agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt aagacaatca
      781 aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc gtaaaaacaa
      841 aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa atatataaaa
      901 atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt ttttgtttct
      961 tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt aaaggttcca
     1021 gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga gaccgaatgt
     1081 ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc atgattggcg
     1141 gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc acagccaggc
     1201 atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt tggataactt
     1261 gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc agggaacatg
     1321 tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta ttttttattt
     1381 atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg ttagctttgt
     1441 gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag ggatcattaa
     1501 aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc caaatataga
     1561 aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg accgaaaatg
     1621 aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat tcataagaga
     1681 aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg tataactttc
     1741 tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata atatcatcta
     1801 atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt gacactcgat
     1861 aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa gctgtgaaca
     1921 aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt tgtaattcgg
     1981 aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga agagaaggta
     2041 cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata cttgattggc
     2101 tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta gaagtacttt
     2161 tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca gaattctttg
     2221 ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg taaatccctt
     2281 tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac ttaaacaaag
     2341 aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc atgaagataa
     2401 taactttttt aataattata gctttctaat atctttcatc aacttttcta gtaatcattg
     2461 ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg atcttcgcaa
     2521 aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac aagcctacca
     2581 acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct aaagttttca
     2641 ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga aacattgctt
     2701 atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt tttgctcttc
     2761 cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt ttttagtgag
     2821 cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg gattggttgg
     2881 atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga agaagtatcg
     2941 tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact gaatgccgta
     3001 tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg gacatagaag
     3061 aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc aataatgcag
     3121 gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag tatgaattat
     3181 cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat ttgatgagcc
     3241 aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat ctttcttctg
     3301 aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa gcagcgatga
     3361 acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt gtggtaggtg
     3421 ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa gaagctcttt
     3481 cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga acaagtacga
     3541 tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc tattatcttt
     3601 cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt aaaacaagag
     3661 gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa ttattgaaat
     3721 tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc aaaaccagta
     3781 atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt attgcaaaaa
     3841 tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt tttgatgaga
     3901 tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa agaatcgctt
     3961 atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat ttgtctgaag
     4021 aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa gagatgatca
     4081 atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata ataggagatg
     4141 agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca gagacgaaaa
     4201 cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag acgaatgcta
     4261 gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat agaatctcta
     4321 gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa tatatccaag
     4381 aattcaatga actgatcaat cagttggaag aaatctatga actaactttg agccaatatg
     4441 aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg atgataagga
     4501 aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt cgacgattag
     4561 atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac ctctctttat
     4621 tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt gagatagaaa
     4681 aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta gaagctatct
     4741 tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg gagttagcat
     4801 ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat acgacaggaa
     4861 aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg gatatacaaa
     4921 taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat ttcgctgttt
     4981 ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat ttgactaatc
     5041 tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag cgattagccg
     5101 ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg tatcaagaat
     5161 atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa gaatttgaaa
     5221 aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa tttgcttttc
     5281 ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta ataactgatc
     5341 aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata cctgaagaaa
     5401 tgacggctga agttgagaat gaattatttg agctatacga tatcctattt gaactcataa
     5461 aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt ttaaaactaa
     5521 tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt tttcgcagct
     5581 tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt caatcaaaca
     5641 tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg gaaaatccgt
     5701 tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga agtcagaaaa
     5761 atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc gggtgaagat
     5821 atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt gacacaagca
     5881 gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga aaaaagtgaa
     5941 caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa aagtacgatc
     6001 aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag ggatttatga
     6061 ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc gtaccagttg
     6121 tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa gaccgagtaa
     6181 cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg ttaccgttct
     6241 tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc cttccagaat
     6301 actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc aatggtgtat
     6361 tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa gggatcgaaa
     6421 ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga ctgctgatga
     6481 actttattgg tggttggata actgatttta ctactggatt tgtatgcaga cagcaaggaa
     6541 tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt tttaaatgta
     6601 tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga ttattaggcc
     6661 gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc caatgatcgt
     6721 agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa catcaatacc
     6781 tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat gtggaattta
     6841 tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg gcccattagc
     6901 acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa tacagccact
     6961 aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata aaaagtacaa
     7021 atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact ggatagcaaa
     7081 aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa gagaagcagt
     7141 agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt tgattggtgt
     7201 gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt tagcaggaaa
     7261 tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat ctcctttgct
     7321 tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag agattgggaa
     7381 aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata ctcaaaatgc
     7441 atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga cagtagaagt
     7501 aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag ttatcattgc
     7561 ttgggtagca agttttggat tatatagctg
//
LOCUS       DAZKNO010000128         7520 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000128 DAZKNO010000000
VERSION     DAZKNO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.130"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            155..1048
                     /locus_tag="ACG8MY_002104"
     CDS             155..1048
                     /locus_tag="ACG8MY_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0431275.1"
                     /translation="MGKISLGIDIGGTFIKYALIDKQHRIKEKWKVETVKYNTADEFY
                     DYICSNIRNVDEIDKIGVSAPGLIDEDSNVKSYAAPNVAIMYGTNINNEINKRTNKKV
                     SAINDAKAAGLCEFKIGNAKGYKSSAFLIIGTGTGGCICDENGVVYGKDSFAGEFHNM
                     PFVNAEIGGLDKMGDYASITGLVNLYNKKANREEQVQYGNEVCKKYLNGNESAVSSVD
                     EWIGNIVAQLIVITVFYNPEVICIGGGISEENWFINKVRETYKKTCREYLEADFITTE
                     INRCKHNNDANILGAVIKASI"
     gene            1122..2561
                     /locus_tag="ACG8MY_002105"
     CDS             1122..2561
                     /locus_tag="ACG8MY_002105"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0431276.1"
                     /translation="MIYEELDKFQDDFLWGSASAAYQVEGAWDKDGKGESVWDVYTRQ
                     PGTTFKNTNGNIAVDHYNRYKEDVALMAEMGLKAYRFSIAWTRIYPNGRNEVNEEGLK
                     FYENLIDELIANNIQPIITLYHWDLPQHLQDLYGGWESREIIHDFNEYCVTLFKRFGN
                     KVKHWVSLNEQNIFVTLGYVTAMHPPAVKDQKRMLEANHIANLANATVIESFKKYVPD
                     GMIGPSFAFGPVYPFSCDPKDVLSAENAEDLNCNWWLDVYCKGKYPVFAFKYYERLGI
                     APTIEDGDLELLERVKPDFIGINYYQTSTVAFNPLDGVGQSEGINNTGKKGTTKESGL
                     PGVYKNAKNAHLETTNWDWAIDPTGLRIGLRRLTSKYNLPILITENGLGEFDKLEEDD
                     TVNDAHRIDYLSAHIKACKEAITDGVDLLGYCTWSFTDLLSWLNGYQKRYGFVYVDRD
                     ENDQKDLRRIKKNSFYWYKNVIRTNGEEL"
     gene            2609..2911
                     /locus_tag="ACG8MY_002106"
     CDS             2609..2911
                     /locus_tag="ACG8MY_002106"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0431277.1"
                     /translation="MKKILLVCSAGMSTSLLVTKMRNSAKEKGIEVEIDALPVAEAST
                     AVDNVDIVLLGPQVRFQKGTVEKLVKGRIPVEVMDMRLYGMMNGKAILEDALQKIN"
     gene            2930..4294
                     /locus_tag="ACG8MY_002107"
     CDS             2930..4294
                     /locus_tag="ACG8MY_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0431278.1"
                     /translation="MSFMDKFQSAIERLLVPLSTKLNAQRHICAVRDAFILSFPLTMA
                     GSLMVLLNNVFLSADGFMFKILQFDKLFPGIVKFQAVFAPVVKGSADIFSILIVFLIA
                     RNLAKLMEGDDLLTGLTAISVYFIIYPDYFDNEGVNYLTNQYMGAQGLFVAILVGLLV
                     GELMSRLAKSDKLEIKMPEQVPPAVARSFKVLIPIIITTVFFSVLNYFVKMAAPGGLH
                     ELIYNIIQTPLTRMSQSLFSVIILALLSQLLWAMGIHGPNTIAAIRDTMFSEAGNANL
                     LHYAESGTTWGAPFPITYSGLATAFAEYGGSGATLGLIIAILIFSKNKESKSIGKLSF
                     VPGLFNINEMVIFGLPIILNPIYIIPFIIAPLVNIMIGYTAVMILKIMPPVTIGIPWT
                     TPGPLMPFLGTGGNIVALFVGFICLAVSVLIYSPFVIAANKAAIIEDEMENEIQSQEV
                     VESI"
     gene            4350..4664
                     /locus_tag="ACG8MY_002108"
     CDS             4350..4664
                     /locus_tag="ACG8MY_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0431279.1"
                     /translation="MEGIELTAFSIISNVGMAKSLAIEALRDARKGKYNEAEKKMAEA
                     SEYFVKGHHAHTSLIQKEAAGEKVELSLIIMHAEDQLMSTETTKLLIEEMIEMFKQLN
                     NK"
     gene            4998..6044
                     /locus_tag="ACG8MY_002109"
     CDS             4998..6044
                     /locus_tag="ACG8MY_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0431280.1"
                     /translation="MMNSTEIAKIAGVSRSTVSRVINNYSNVSEETREKVLRVIKEHE
                     YVPHASARMLAGSQNKVIGLFIIDLIHEEVGLKNRITKSPYYLEFTSSVIETASEMDY
                     LVLVHIIHSVAGYEKIKESFYNKTISGGIFIGQNDDDESIKTIIDRGYKVVLLDQSIR
                     PEDATYNQCMIVNADNYNGAYCATKYLIDKKHKQIAHITGGTVKFSSKDRIRGYKKAL
                     EDAGIEVNKNLIVNSEFVENAGYEAAKKLFNGNSEFSAIFASNDKIAFGAIKAIQERG
                     LKVPDDISVIGFDDIESSKYFNPPLTSMKMKLGEMADIATKSIVASIEGETKPSINVV
                     PVSLVERESCGILQ"
     gene            6599..7153
                     /locus_tag="ACG8MY_002110"
     CDS             6599..7153
                     /locus_tag="ACG8MY_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431281.1"
                     /translation="MKQLLSKLKKIWAVLPEHSLKQRIGNNLALIYSGALLIQDILGY
                     PLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTI
                     WGKVSIKKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRI
                     HMNRQVLPTYEILLPSSLKSYFRL"
     gene            7202..7357
                     /locus_tag="ACG8MY_002111"
                     /pseudo
     CDS             7202..7357
                     /locus_tag="ACG8MY_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         2615 a         1172 c         1596 g         2137 t
ORIGIN      
        1 acgcgcgttc atgggatgtt catcgtataa aaaaaggtgg aaatgagcgt atcgtttttc
       61 tttttaacaa atcatgaacg cgcgtttatg agtttgaatc aagctgcaat ataagaaggc
      121 aaactgcaag accttcattt taaggagaag tgttatgggg aaaataagtt taggaataga
      181 cataggaggg acgttcatta agtatgcatt aatagacaag caacatcgca tcaaagagaa
      241 gtggaaagtc gaaacggtta agtacaatac agccgacgaa ttttacgatt atatatgttc
      301 aaatataaga aatgtagatg aaattgacaa aatcggtgta agcgcacccg gccttattga
      361 tgaagattcg aatgttaaat cctatgctgc tcctaacgta gctataatgt acggcacaaa
      421 tatcaataat gagatcaaca aaaggacaaa taaaaaagtg tccgcaatca atgatgcaaa
      481 agcggcaggt ttatgtgagt ttaaaattgg aaatgcaaag ggatacaaat cttcagcgtt
      541 tttgattata ggtacaggaa caggcggatg catatgcgac gaaaacggag ttgtgtatgg
      601 aaaagattcg tttgcggggg aattccataa tatgcctttt gtaaacgctg agataggcgg
      661 ccttgataag atgggtgatt atgcttcgat aaccggattg gttaatttat acaataaaaa
      721 agctaatcgg gaggagcagg ttcagtatgg aaatgaggtt tgcaagaagt acctaaacgg
      781 aaatgaatca gctgtgtctt cagtagatga atggatagga aacatagtgg ctcaattaat
      841 agtaatcacc gttttttata atcctgaagt gatatgcatc ggtggaggaa tatccgaaga
      901 aaactggttc atcaataaag taagagaaac gtataagaaa acctgtaggg agtatttaga
      961 agcggatttt attactaccg aaatcaatag atgcaagcat aataacgatg ccaacatact
     1021 gggtgctgtt ataaaagctt ctatctaaaa tacacaacag ttttgtttca agaaagaatg
     1081 tttgaaaata aatttatatt gaaaacgaag gagaagattc agtgatatac gaagaattag
     1141 acaaattcca agatgatttc ctatggggat cggcatctgc agcgtatcag gttgaaggag
     1201 catgggacaa agatggaaag ggggaaagtg tatgggatgt gtacacccgc caaccgggga
     1261 caacatttaa gaatacaaac gggaatatcg cggtcgacca ttacaataga tacaaagaag
     1321 acgtagcttt aatggctgaa atgggattga aagcttatag attctcaatt gcatggacac
     1381 gtatttatcc caatggaaga aatgaagtaa atgaagaagg cttaaaattt tatgaaaact
     1441 tgatagatga acttatcgcg aacaatatcc aaccaatcat tacgttgtac cattgggatt
     1501 tacctcaaca tcttcaagat ttgtacggcg gatgggaatc aagagaaatc attcatgatt
     1561 ttaatgaata ctgtgtaacg ttattcaaga gatttgggaa taaagttaag cactgggtat
     1621 cattgaatga acaaaatata tttgtaacac taggttacgt aaccgctatg cacccgccag
     1681 ctgttaaaga tcaaaagaga atgcttgagg cgaatcatat agcgaactta gctaatgcta
     1741 ccgtaattga atccttcaaa aaatatgttc cagacggtat gattggacca agctttgcat
     1801 ttggaccagt ttatccattc agttgcgatc caaaagatgt tttatccgca gaaaatgcag
     1861 aggatttaaa ttgcaattgg tggctcgatg tttattgcaa aggtaaatat ccagtgttcg
     1921 cttttaaata ttacgaaaga cttgggatcg cacccactat tgaagatgga gatcttgaat
     1981 tgttggaaag agtaaaacca gattttatcg gaataaatta ttaccaaaca agcacagttg
     2041 cattcaatcc gcttgatgga gtagggcaat cggaaggtat caataacaca ggtaaaaaag
     2101 gcaccactaa agaaagcgga ttacccggag tttataaaaa tgcaaaaaat gcacatctgg
     2161 aaacaacaaa ttgggactgg gcaattgatc ctactggttt aagaattgga ctaagaagat
     2221 tgaccagcaa atataatctt ccaatactta ttacagaaaa cgggcttgga gaattcgaca
     2281 agcttgaaga agacgacact gtcaatgatg ctcacagaat cgattacttg agcgcacata
     2341 tcaaggcttg taaagaagca ataacagatg gtgttgatct tctaggctac tgcacgtggt
     2401 catttacaga tttgttaagc tggttgaatg gttatcaaaa acgctatgga tttgtgtatg
     2461 tggacagaga tgaaaatgat caaaaagatt tgagaagaat caaaaagaac agcttctatt
     2521 ggtacaaaaa tgttattcgc accaatggag aagagttata agaatcgttc acaatcaatg
     2581 aatatgaaaa aacaaggaga gatagaccat gaaaaaaatt ttactagtat gttcagcagg
     2641 aatgtccaca agtttgcttg tgaccaaaat gagaaactcc gcaaaggaaa aaggaattga
     2701 agtagaaata gatgctcttc cagttgcaga ggcaagtacg gccgttgata atgtcgatat
     2761 cgttctttta ggaccacaag tgcgtttcca gaaagggacc gttgaaaaac ttgtaaaagg
     2821 cagaattccc gtagaagtga tggacatgcg gctgtatgga atgatgaatg gaaaagcgat
     2881 tttagaagat gctttacaaa aaataaacta ggaaaggagg ctaaaaaaaa tgagttttat
     2941 ggacaaattt caaagtgcta tagaaagatt gttagtgcct ttatctacaa agttgaacgc
     3001 tcaaagacac atatgcgcag taagagatgc ttttatttta tcattcccat taacgatggc
     3061 aggatcattg atggtgttat tgaataatgt tttcttgtca gcagatggat tcatgtttaa
     3121 aatcttgcaa ttcgataaac tttttccggg tattgttaaa ttccaagcag tgttcgctcc
     3181 agttgttaaa ggttcagctg atattttctc gattctgatt gtgtttttga tagccagaaa
     3241 ccttgcgaaa ctaatggaag gcgatgattt gttgacaggt ttgaccgcaa tctctgttta
     3301 tttcatcatc tatcctgatt atttcgataa tgaaggagta aattatctaa caaatcaata
     3361 tatgggcgca caagggttgt ttgtagcaat tttggttggg ctcttggtcg gcgaactaat
     3421 gtctagatta gcaaaatcag ataagttaga aattaaaatg ccagaacaag tacctccagc
     3481 agttgcaaga tccttcaaag tgttgatccc gattatcatc acgacggttt tcttctccgt
     3541 cctcaactac tttgtaaaaa tggctgcacc gggcggatta catgaattaa tttataacat
     3601 cattcaaaca ccattgacca gaatgagtca aagtttattc tcagtgatta ttttagcatt
     3661 attatctcaa ttgctatggg ctatgggaat acacggacca aatacgattg cagctattcg
     3721 agataccatg ttctcggaag ccggaaacgc aaacctctta cattatgctg aaagcggtac
     3781 tacatgggga gcgccttttc cgataacgta cagtggttta gctactgctt tcgctgaata
     3841 tggcggttca ggagcgacat tgggtctaat cattgctata ttgattttct cgaaaaataa
     3901 ggaatcgaaa agtattggca aactttcttt tgtacccggt ttgttcaata taaacgaaat
     3961 ggttatcttt ggtttaccga ttattctgaa tccgatttac attattccat ttattattgc
     4021 gccactggtt aacattatga ttggttatac cgcagttatg attcttaaga ttatgccgcc
     4081 ggtaacgatt ggtattcctt ggacaacacc aggaccattg atgcctttct tgggtacagg
     4141 cggaaatatc gtcgcactgt ttgtcggatt catctgtctt gcggtgagtg tattgattta
     4201 ctcgccgttt gtaatcgcag cgaataaagc agcgattatt gaagatgaaa tggagaatga
     4261 aatacagtca caagaagtag tagaatcgat ttaaaaaagc tgcagtacgc tgagaacaaa
     4321 ccgaaagatt caagatagga gatttacaca tggaaggaat tgaattaact gcttttagta
     4381 taatcagtaa tgttggaatg gccaaatcat tggctattga agcattaaga gatgccagaa
     4441 aaggaaagta caatgaagca gaaaagaaaa tggctgaagc atcggaatat tttgtgaaag
     4501 gccatcatgc acatacttct ttgatacaaa aagaagctgc tggcgaaaaa gttgaacttt
     4561 cgttaataat catgcacgca gaagatcaat tgatgtcgac ggagacaact aaattattaa
     4621 tcgaagaaat gatagaaatg ttcaaacagt taaacaataa ataagtgaac atgaaagaga
     4681 aaatattaga cgaactagaa ttgcttgctt actttacagg cagaatctga tgccagcagc
     4741 aaaacataga gcacccagga tgaaatataa tcctgggtgc cttattttta gaacaaaaca
     4801 ccggggtggt agtttgttct gcggttgaag gcatttgctg atggtttctg catgaaaggt
     4861 attgccaaaa gtctcctttt gctggaatcc tgaagcaggc agagtttttc gaaaaacaga
     4921 aaaagattga aaaaaatgga caaataatat agtgttgcag tatgaacacg agtttacgaa
     4981 cgaaaggaga tgcctttatg atgaatagta ctgaaattgc aaaaattgcc ggagtatcac
     5041 gcagtactgt atccagagtg ataaataatt attcaaatgt ctcggaagaa acaagagaaa
     5101 aggttttaag agtaattaag gaacatgagt atgtgccaca tgcttcagca aggatgcttg
     5161 caggaagcca aaataaggtg atcggcttat ttattataga cctgatacat gaagaagttg
     5221 gactaaaaaa tagaattacc aaaagtccgt attatctaga atttactagt tcagtgatag
     5281 aaacagcaag tgagatggat tacctggttc ttgtccatat tatacatagc gtcgcaggct
     5341 atgaaaaaat taaagagagt ttttataata agacaatttc tggtgggatt ttcataggcc
     5401 agaatgacga cgatgaatcg ataaaaacaa tcattgatag agggtataaa gttgtgctgc
     5461 tcgatcaatc aatcagacct gaagatgcca cctataacca gtgcatgata gtgaatgcgg
     5521 acaattacaa cggcgcttat tgtgcgacaa aatatctcat agataaaaaa cataaacaaa
     5581 tcgcacatat tacaggcggt acggtcaaat tctcttctaa agacaggata agaggataca
     5641 aaaaagcatt agaagatgca gggatagaag taaataagaa cttgattgta aatagcgaat
     5701 ttgtggaaaa tgctggatat gaggctgcga aaaaattatt taatggaaat agtgagtttt
     5761 cggctatttt tgccagtaat gataagatag catttggagc aatcaaagct attcaagaaa
     5821 gaggtctcaa agttccagat gacatctctg ttataggatt tgatgatatt gaatcgtcta
     5881 aatattttaa tccgccattg acttctatga aaatgaagtt aggagaaatg gctgatatcg
     5941 ccacaaagtc gattgttgca tcgattgaag gtgaaacgaa gccatctata aatgtagtac
     6001 ctgtctcatt agtggaaagg gagagttgtg ggattttgca gtagcaaaac ttgctgaatt
     6061 agttggagct gattatttga ttattttgat tgaggtagac aatgtctgtg tctatgtcga
     6121 cttgaaaagt aaagtacagc gcccaaaaat gctctgtact ttaaatctca gcaaaaacag
     6181 gaatatttcc caaacatctc cttctttgag taattataca aaaattatgc agactttctt
     6241 ttttcatgat aagataattt aaaaagtttt taaacgattt ctcctcctct ttttcttata
     6301 tcgggggagc atattttttt gtagactagg ggattataag ttcagccatc aatgtctagc
     6361 tcccaagtcc tgacctagtg aaaaaaagat aaatttgccc cattgcgcgc ttcgctgctc
     6421 attcagggtc aaatttccta ttttttcata ggtcaaggcg gacttgtccg cttttcttat
     6481 attaagttta caaaggatgc gaatcacgca gagaaagtta aaagtctttc ttctaatact
     6541 tttggttggg gtataaagtt atttcttttg aaactctttg aacaagggat tgcaaaaatt
     6601 gaagcaactt ttgtcgaagc tcaaaaaaat atgggctgtt ttacctgaac attctttgaa
     6661 gcaacgcatt ggtaataatc tagctttgat ttatagtgga gctctcctaa ttcaagatat
     6721 actgggctat cctttacgat tagagcatat acagacggtg cttattaatt cgtacgaaca
     6781 gttaatcgta gatactagtt taggaagtga gcaaacggta tttgaccgag tgaaaacatt
     6841 gttggtgatg aatagccata aatttttaac aaacagtgac aacaaacata cgcccagtac
     6901 gatttgggga aaagtatcaa ttaaaaaaaa tggggatatt aaggtaaata tttttcctat
     6961 cccgtttgag gacttgttaa ggcgagaatt tcaagtaaag gatctaacct atctttttag
     7021 acaattgctt gattcgggta atatgtgtgc agaaaaaggg agaagaacga aacgtattca
     7081 catgaatcgt caagttcttc caacatacga aattctatta ccttcttcac tcaaatcgta
     7141 ttttcgattg taatttagaa taaaccaaag tatgaaagtg aggcaccatg ttattaagca
     7201 actgaaagat attcctattc caatgcccgc tatttcagaa caacaaaaaa tcggcacttt
     7261 cttcaaacaa ctcgacgaca ccattaccct tcaccaacgt aagttagatt tactaaaaga
     7321 aacgaaaaaa ggcttcttac aaaagatgtt tgtttaggag ggaagaaaaa tgaaaattag
     7381 tcaccgtgag gaagcggaag tcgaagaatg atggtgacac tcacaatgta ataaaaggtt
     7441 ttatgcccct tgatctatca actgggagaa aaattcgatt ttcagaaatt cagtatgaag
     7501 gaattggttt tactcttata
//
LOCUS       DAZKNO010000129         7472 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000129 DAZKNO010000000
VERSION     DAZKNO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.131"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..656
                     /locus_tag="ACG8MY_002112"
     CDS             <1..656
                     /locus_tag="ACG8MY_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0431282.1"
                     /translation="LLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYA
                     FLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGID
                     PTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGA
                     LYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            668..1588
                     /locus_tag="ACG8MY_002113"
     CDS             668..1588
                     /locus_tag="ACG8MY_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0431283.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1629..3086
                     /locus_tag="ACG8MY_002114"
     CDS             1629..3086
                     /locus_tag="ACG8MY_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0431284.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3431..4045
                     /locus_tag="ACG8MY_002115"
     CDS             3431..4045
                     /locus_tag="ACG8MY_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0431285.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4038..5762
                     /locus_tag="ACG8MY_002116"
     CDS             4038..5762
                     /locus_tag="ACG8MY_002116"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0431286.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5759..7210
                     /locus_tag="ACG8MY_002117"
     CDS             5759..7210
                     /locus_tag="ACG8MY_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0431287.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2549 a         1253 c         1486 g         2184 t
ORIGIN      
        1 atctgctgat ctcagtcgta tttgcaatca ttatgagcga gttgagaaat aaacgactcg
       61 ttaaagttta tcaaacgatg tccttactac cttattttct atcttgggtc attatcagtt
      121 actttgttta cgctttttta agtcctgaca aaggaatatt caatcagtgg atcactggac
      181 atggaggaga agctgtcaat tggtataatg aaccgaaata ttggccgttt atcttggtgt
      241 tcattggtac atggaaaggg attggttata acagcattat ttactttgca tctgttatgg
      301 gaatcgatcc gacttattat gaagctgcta tggttgatgg agctagcaaa tggcagcaga
      361 tcaaacatgt gacgatacca caacttattc cactaatgac gattttaacg attttagccg
      421 taggaaacat tttccgagct gattttggcc tgttttataa cattcctagg aattccggtg
      481 cattatatga tgtgactcaa gttttggata cgtatatcta caatggcctg acatccacag
      541 gagattttgg tatgacggct gctgcaggac tttaccaatc agttgtcggc tttgtcttgt
      601 tgatgatcac aaatacgatc gcccgacgtt ttgatagcga ttctgcatta ttctaaggag
      661 gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc cgctctttca ataagacggc
      721 taattttttc tctaatataa tgattgcact ctttgctatt tcctgtattt tgccatttat
      781 atttgttatc gccatctctt ttaccgatga gtccatattg tcgactcacg ggtacagttt
      841 ttggccagaa aagttcagta tatttggtta tacgtacttg ttcgaccaga tgcaggataa
      901 aattttccaa gcgcttttcg tcactgtact tgttactgtt ttaggtacac tgatcaatag
      961 cacggctact tctttatatg cttatgcgat ttcacggtca aactttccat tccgtcgttt
     1021 ttttaccgtt ttttgtttga ttacgatgtt gttctcacca gggatggtgg ccaattatct
     1081 tgttatgaca aatcttcttc agttaaaaga tacgatttgg gcattgatct tgccaatggc
     1141 tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt aaacgttcgg tctctgattc
     1201 gattattgaa tcggcacgga tcgatggagc tagcgaactg cgaatatttg ttcagatcgt
     1261 tttaccgtta gctgttccag gtattgcgac aatcagttta tttgccgcat tgggttactg
     1321 gaatgactgg ttcaatgctt tactatacat ccaagatgat aagctagttc cactacagta
     1381 tcttttgatg aaaatccaaa gtaatattca atatctgaca caaaattcag gagcaggaag
     1441 ccaattgaca ggcggattag cctcagttcc aggagaatct gccagaatgg cgatcgtagt
     1501 catctcgacg ttgcctattg cgatcagcta tccatttttc cagaagcatt ttgtcaaagg
     1561 tctaacaatc ggcggagtaa aagaataaag aaacaaaaat ataaatcata aaattaggag
     1621 gaagaaaaat gaagttatgg aagaaactag cctttacagg tgtttcagcg ttgttgttgg
     1681 gaacattagc agcatgcgga ggttctggtt ctaaagacca ggcagaagct agcaattcgg
     1741 acactttgca gatgtatcaa atcggagata aaccagataa tttcgatcaa ttgatggaag
     1801 tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt caatatcaat tacattggct
     1861 ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc cggggaaaat tatgatatcg
     1921 cctttgcgaa caactatgtt tccaatgccc aaaaaggagc ctttgctgat ttgactgaat
     1981 tagcgcctaa gtacgctgaa aaagcttata atgatttaga tgaagcatat attaaaggaa
     2041 atctggtcaa tggaaaatta tatgctttcc cagtcaatgg aaatgtcttt gctcaacaag
     2101 tacttacttt taacaaagct ttactagata aatacgatct ttcaattgat ggaatccaat
     2161 cttacgctga tgcagaaaaa gtcttgaagg aattccatga aaaagaacca aacacggcag
     2221 cttttgccat tggacaaggc ttcaaagtcc aagggaactt tgattatcct ttagggaata
     2281 cgttaccatt tgctattgat ctggatggag atgattcgaa aatcatcaac caatacgata
     2341 atgaaacgta tatcggactg ttacgtacaa tgcatagctg gtacaaacaa ggactcattc
     2401 caagtgacgc tgcaacgagc aataaggatt atccactaga aggaaatact tggctaatgc
     2461 gtggagaaac acaaggaccg tatgattatg gcgatacaat tttgacaaat gcagcaaaac
     2521 aagaacttgt ctcaaaagca attactgttc cactaaaatc tacagctcaa gcacaaatgg
     2581 caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa atcagtagaa cttctagggc
     2641 tgcttaacag tgatccagaa ttattgaatg ggcttgtttg ggggatcgag ggagaagcat
     2701 gggaaaaagt cgatggttca gatgataaaa tcaaattgct agatggttat cagccaaata
     2761 cacatatgtc tgcttggaat acaggaaaca acaagattct atatacacaa gagtctatta
     2821 cggatgacat gattgccaaa cgtgatcaat cgattgctga tgcagaaact tcaccgattt
     2881 taggattcag cttcaacact gacagtgtca aaacagaatt aagtaatatt tctaatgtca
     2941 tgaatcaata tttagatgga ttgaatacag ggactgttga tccagatgaa acattaccga
     3001 aattgaaaga cgctttggac aaggctggct atgataaggt attgaaagaa atgcaaaaac
     3061 aatatgatga gttccgacaa gaataataat gaataaagac aaaggggttg tgacaaaaga
     3121 cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa gccagtttct cggtattaag
     3181 ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa tttttcttac tactcgaaac
     3241 taagcggcta tttcacaaac tcttggtaaa cggtgttcaa tcagctaacc ctcggtattc
     3301 gctcacaaaa cgacaaaata tgaggaacta tttttccgtt tgtttctcta ataatcaggt
     3361 caaggtgctg attgcccaac ctttttttgt ataatgagaa agtataaact taatgtttgg
     3421 aggagaagat atgttaggga aggttttgga agagttattt atccggatct gggtcattat
     3481 aaaactaacc ttgtatttct ggatatatac atttgccggc gggatcatct ttggattagg
     3541 tgcggcatgg aaaacagtca atgaactatt ttatttgtac ggattcgagt ataaagaaat
     3601 cacaataaaa agaggctgga atatatataa aagaaatttt ttaagaggca atctgctatt
     3661 tagtttattt ttatctggta ctgctttatt gagttacaat ttatatttat ctgttcaaat
     3721 caaagggctc atttttttag ctattgattt tattttactt tttgccttgc tctgcctatt
     3781 tgctacttat caatacagta tgattttaga tagtgaatat acgatctctg ttcctaatct
     3841 gctaaagcta agttttattt cggttttttc cagcttttcg tcttttctaa aaataattat
     3901 tggtagcggt ataattttag gcgtgacttg gcaattcaaa ggattgatcc tgtttggtgt
     3961 cattggtctt ttgacggttt ggaatggtac gatgacaaca cattggcgtg aagaattaga
     4021 taaacagttg gaaagttatg aataagtttc ggattctatt ttcaaaagga accttactaa
     4081 atcgtctgtt gcggaaatac gcggttatca tgatcggtgt aacgatgact gctaccgtta
     4141 tttttagtat ccatacttgg gaacaaaatc aaaaacaggc agaaaacatg acaagtgatg
     4201 ccgttcaaag cacaagcaga atgctgaatg ataaaacaac cttgagtagg atcatcaaaa
     4261 accagcttgt agggaattcc gaaaagatcg aaaatgtgac gacctactta acaaaaccca
     4321 tagatcagta tctgatgtat gtatacgaac agcaaaacag taccgatgaa ttagtttctt
     4381 tcccaaatca aatcaaagat ctctacgcaa attatgaaga actaagtgcg atatatatcg
     4441 tgttgaatca actaccggag tattatgaat cttctagaga aaacaaaggt ggggaaataa
     4501 aaaaggggac accaaaatta ctagacgcct tttatatcaa gttgcctatc acacaaggag
     4561 gagctgagtc aggaagtatt tttattggtt ttcaaaagaa agaactagat atgattttag
     4621 aaaatctaac gtcattcaat ggactctctt tatacatgat ttctggaacg acaaatcgac
     4681 tatacacatt ccatgataat aaaatagcta aagaaacgtt tgaaagacaa gaaatgatca
     4741 tcacagagag tctgaagaaa aattcacaat tgccgattga cgatctggaa aaaaacaatt
     4801 ttgttcaaca ccaagaactg tctgatgatt atcgtatatt agctgtacta gataaaaaaa
     4861 gtgtaaaaat cgaaacggga aaaaatcttg ccccgctgat cattggtatc attattttag
     4921 acaatatact gcttatcttt ctttataaaa cattcaaacg atattcacag caagtatcta
     4981 tcgtcatgca ggccatggat caaacagcag cagggaattt agagacgcgg atcgacataa
     5041 caaacacgga atatgaactg aaggaacttt caatcggtat caacgaaatg ttggatagca
     5101 tcaatcagtt tgtagaagac atctacaaat tagaaatcaa acagcaagac gcacatatgc
     5161 gggcgcttca agcacagatc aatccacact ttttatataa tacattagaa tatatccgaa
     5221 tgtatgcaat cagcgaagga agtgaagaat tagcagatgt tgtgtatgcg ttttccgctt
     5281 tgcttcgaaa caatacgaat caagagaaaa cgatcacttt aaaagaagaa ctggactttt
     5341 gcgaaaaata tgtctatttg tatcaaatgc gttatcctaa tcgagtagcc tatcatttta
     5401 tgatcgatcc tgatttggaa aagatcgaag tacctaaatt tgtgatccag ccattagttg
     5461 aaaattattt taaacacggt attgatttta ctcgattcga taatgcgcta agtgtcaaag
     5521 tcttgcagga aggcaaacgg gtaaggatca tcatcaaaga taatggaaaa gggatgactg
     5581 aaaaacggct aaaacagatt gaagaaaaac tgtctcatcc taaagtggaa cttcacggtt
     5641 ctatcggtct gcaaaacgtg aatgaacgtt tacgtgccag cttcggcagc agttattata
     5701 tgtcgcttga gaataatgag acaggtggac tgaccgtttc tattacattc aaggagggat
     5761 gagcgtgtat caagtgttat tggtagatga tgaatacatg attttgaatg ggttaaaaaa
     5821 gataattgat tggcaaagtt taggctttca aatagtagca acagcagaga atgccaaaga
     5881 aggactagct gtacttgaac agcgacagat tgatttagta gtaacggatg tgacaatgcc
     5941 agaaataaat ggactggaat ttattgaagc ggctcaaaaa gaacgtcata attttgaatt
     6001 tatgatttta tcaggatacc aagaattcga ttatttgaaa ggaggtatgc aattaggcgc
     6061 cgtgaattat ttgatgaaac ccgtgaataa gttcgaattg gttgaaagcc tcaagaaaat
     6121 caaaacacgg ttagatcagc aaaatgaaca aaaaaatcaa caagaaatct atcaagaact
     6181 gttattcagt caatggttga atgaagaatt ggatgaaata agtgaagaag aaataatagg
     6241 acaaataagt gagaagcagc ataggatagt attgattcaa ctggcaagaa ctcatgatac
     6301 tcttatcaat agatggttgc aagaacggca acaaatcttt tactatcaac gaaattacgg
     6361 agaccgtgta ttgatcacgt tgttgttgga ggcagatgaa gttgatggct tctgttgttt
     6421 tgttcaagaa aactttcctg atcaggaatg gctgatcagt atcggggaag agacagattc
     6481 tttggacaaa atccctgaaa cattccaaca agcgaaagac aatctacagt tgcatcaatt
     6541 ttatggagat aaagacgagc atgtttttta cgcagtacaa gcagaaacaa aagatcaaac
     6601 catcgatttt tcaagtttca gacgcgtgct gcaaaataaa caattggaag tcgctcaaaa
     6661 aatgatcact gatttttttg aacagtttca gttagcagcc atgatgccgg aagatatacg
     6721 atattccagt tttttactgt ttatggagat tcaacgggaa ttgattgatc tggaagatga
     6781 ggaatattta caaggaatcg aaaaaatcca tcaggcaaaa acggtgcaag aacttcagca
     6841 gctacttctg tcattcatca aaaagcatca gagacagaaa aagtacagtg acaatgtgga
     6901 aaaagtaatc gtaatcctgc accagcatta tcaagaacca cttacactca aagaagtgtc
     6961 agaaaacctg catttaaatg tcatgtatct aggacagctg ttcaaaaaag aaacgaaaaa
     7021 aagtttttca gcttatttga accatttacg tatggaaaaa gcgaaacaac tgcttttgca
     7081 cagtaatcaa aacatcaatg agatagcaag tgagattggc tacaacaaca ccacgtattt
     7141 ctctaaatta ttcaagaaaa tcgtcggcag atcccctaaa gagtaccgag aaaacagtgg
     7201 agaatcataa aagaaaaagc caatgtaatg aaataactta taaaaaattg tctagaaatc
     7261 taattttggg ctgttcttat tacttgaggc aaggcagctt tttcatgacc tctcgataaa
     7321 cggagttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtggaaagcc
     7381 attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacga cctctccact
     7441 aaacggtgtt caatcagctg accctcggta tt
//
LOCUS       DAZKNO010000130         7418 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000130 DAZKNO010000000
VERSION     DAZKNO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.132"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(274..1239)
                     /locus_tag="ACG8MY_002118"
     CDS             complement(274..1239)
                     /locus_tag="ACG8MY_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0431288.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1473..2318
                     /locus_tag="ACG8MY_002119"
     CDS             1473..2318
                     /locus_tag="ACG8MY_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0431289.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YPLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2509..3696
                     /locus_tag="ACG8MY_002120"
     CDS             2509..3696
                     /locus_tag="ACG8MY_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0431290.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYFYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVVGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3702..4058
                     /locus_tag="ACG8MY_002121"
     CDS             3702..4058
                     /locus_tag="ACG8MY_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0431291.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            4060..4398
                     /locus_tag="ACG8MY_002122"
     CDS             4060..4398
                     /locus_tag="ACG8MY_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0431292.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4474..4730
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4793..5827
                     /locus_tag="ACG8MY_002123"
     CDS             4793..5827
                     /locus_tag="ACG8MY_002123"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431293.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5820..6506
                     /locus_tag="ACG8MY_002124"
     CDS             5820..6506
                     /locus_tag="ACG8MY_002124"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0431294.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6530..7378
                     /locus_tag="ACG8MY_002125"
     CDS             6530..7378
                     /locus_tag="ACG8MY_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0431295.1"
                     /translation="MKKKIFGLAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         2445 a         1324 c         1621 g         2028 t
ORIGIN      
        1 ctttttcacg acctcttggt aaacggtgtt ccagaagtag ctttttcgga aataagccga
       61 aatttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctggc
      121 cacttctttt acgacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc
      181 ccaaccaatt caaaaacatg aacagctgtt tttgtttggc cgttcttatt acttgatgca
      241 gaacagcttt ttcacaacct attgaatcga ttattaagcg atttttttag aaacaaattt
      301 tccatgtttg ctatgttctt taattaagaa acgtacgtaa tgagcaagaa cgtagaagac
      361 aactacactt gcaaacagac attccgcaac ttttaagaaa tccaatacgc tactatagat
      421 acctaaactg ttgaagtaat gaacagtagt aaatatagct gttgcagaga taacaagcgg
      481 gaatgtaaag gctgcatagc ttggataaaa acgaagcttg aacaaacgtg gaagcataga
      541 cagcacaacg aaatacaata cttgagataa agcgaacaat gctacgacaa gataaaaatt
      601 aggttcagca aagtttttaa gataacctgt taggcaaaga gaaccaggtg ctgcaacgat
      661 cgtaatcagc ggacgtgtcg gatgagccat atttcttacg aaaaagacac gtttgatgat
      721 gatcggcaat aagaacgcat agcagatcaa tgaaatccaa aagaaaattt ttccaataag
      781 cgggaagaag ttcccggcag ttacagtgat gaccccaaat ccaacataaa tgataaacca
      841 gcttggataa attgcttcaa ttgaaagatc agctttgata acatggtgat aattgaaata
      901 aaagacaagg atcacttgga agataacagc gcacagccaa atgaatttga caattggtgc
      961 aatactttca tatgaaacaa aataagtaca aataaccatg agtgacatcg taaaagttgg
     1021 tgaaacggag gcgatgattg gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa
     1081 caatttaaca aaaatgaata tcataagaat catagagata ccgccagtta gattgcctaa
     1141 agcaacatgg tgatagtctt taaacagatt tccaagggaa gctaacccaa ggatcaaccc
     1201 gcagatcggg atcggaataa ttttcaaaaa gttcttcata tataatattc tcctttttat
     1261 ttaatagatt ttagtaccaa gatgaacgaa tacattgtaa aatgaatagg aatgtatgat
     1321 tacgagcaag cagacagctg attgtcttca tctaagttac tagttggatc agcaactgtt
     1381 ttgttgtaca tttctattat agatggtttt ttgtatattt aaatcaaata gtttttatag
     1441 taattatcat aaaaagttat ggaagtggtg ccatgtttaa gttattaacg acttttcggg
     1501 cagtatatga aacaagaaat ttttccagag cagcagaagt actgttttta tctcaaccag
     1561 cagtctcgaa tcaaatcaaa cagttggagc gggaattgaa tatcgatctt tttcaacgta
     1621 acggaagaaa agagatgctt ccaactaagc aagccgactt attatatgaa cggacactgg
     1681 ttttgttaga agaatgggaa gaaactcagc aaaaattgct caatgaacaa aatgaagaag
     1741 aagtatgtcg aatcgttgct tcccacacct ttgctgtcta tcctttgcct cagttgatga
     1801 aacatctgat caaaaggttt cctaaagtgc attttgagat cgaaatcgcg aattctcatg
     1861 aatcattaga acaagtagcg aaacatgaat ccgattttgg ttttatcgaa aagccattag
     1921 agacttccgg cgtacaaaga tactctttga tggaagacca gctagtcatc gctggggacc
     1981 caaaaagcaa attgtggctt gtaagagaag caaactctgg tgtttttcat tacacgcagc
     2041 gttattttga agagatgaat atccaagata agaagatgat cgtgaaaagt aacgatgtaa
     2101 ttgttgcgtt gctgaaggaa ggaatcggtc gctcgatttt gtctaagcgc tctgttccag
     2161 aaaatgtgcc aatgactccg ttgaatttcc atcgttattt ttatttcatc caacgaacca
     2221 atctggtttc tgatcagctg ctagagattg ccgaagagat ccttcgatac aatgaaagca
     2281 cagaaacttc tcacagcgag gagaatctca gttggtagat aattgcatgt atcaaaaaaa
     2341 gttctatgat acactaaaga taagcagtag gcttttgtac tgcatttgct caagcaactg
     2401 cacaaacgga aagttaattt ttcccaaatg tctatggaaa gactttccat tatctaattg
     2461 gaccgctata ataatcagca gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa
     2521 ttaaaagctt cgaacgataa tcgtattgat tatggcgtga ttttaccagt ctttctgtta
     2581 tgtttgattg gtctgctctc attatatgta gcacttagcc atgatccgaa caatccagca
     2641 gtcttgaaag gcgtagggat gcaagttgtc tggtatttag ttggagcggc agcaatcgtt
     2701 gtcatcatgc atttgaattc caaatggatc tggaaactga ctccttactt ttatggtttg
     2761 gggttagtgg taatgggact cttgctcaaa ttctatgatc agaatttagc tgcttcaaca
     2821 gggtctaaaa actggtttcg attcggtgcg tttactttcc agccagcaga gttgatgaag
     2881 attgcgtaca tcttgatgat ggcactgatc gtaactaaac ataatacgca agtaaaagaa
     2941 cggacgatga aatcggactt ttggctgatt gggaagctct tgatcgtgac aataccggtt
     3001 ttagcattga tcatggccca agatgacttt ggaacgatgc ttgtctttct tgcgatcttt
     3061 ggcgggattt tcttgatgtc tggaatctct tggcgcatca tcgcacctgt agtaatcttg
     3121 gcagtagtcg ttggagcagg aacgatcttc ttggttacga ctgaaggcgg aagagacttg
     3181 ctttacaaag ttggcttcaa atcttatcag tttgcacgta ttgattcatg gctggaccct
     3241 ttccatgata cgtcaggaat gagttatcag ccggcccaag gacttttggc aattggtacc
     3301 ggtggcttgt ttggtaaagg attcaacgta agcaatattt atgtgcctgt tcgtgagtcc
     3361 gatatgattt ttaccgttgt cggtgaaaac ttcggcttta tcggtggtgc atttgtcatt
     3421 ttcttgtatt ttattttaat ctatcgtatg attcgggtgt gtttcgatac gaataatgaa
     3481 ttttacgcct atatcgcaag cggtttgatc atgatgctgc tgttccatgt ctttgagaat
     3541 attggagcaa atatcggatt acttccttta acagggatcc cattgccttt cattagtcag
     3601 ggaggatctt cgattttagg gaacatgatc ggtatcgggt tgatattatc gatgagatac
     3661 caaaatgaag cacctgcaag atcttcaagg aggtaagaaa attgtacacc ttttactggt
     3721 acccgaaatg ttcaacatgc aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt
     3781 atcaaactgt tgatatgatc aaagaagtac cgaaggcctc aacgattgag acttggctag
     3841 aaacgagtca agtaccaatc cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac
     3901 ttggactgaa agatcaagta ggcgagttta cggcttcaaa agcgagccaa gtcttatcga
     3961 cagatggtat gctgatcaag cggccactga tcttacgaga ggaccagctt gttgcaattg
     4021 gattcaatga aaaaatttat gaaggagaac tgatataaaa tggcaggcaa gcatttgaag
     4081 aaaaaggaca acctttggat cttatttaat ggaaaagaat actgtgttgg tttgacaaat
     4141 gaagcgcaag aagaacttgg agacatcacg tttgccaatc tgccaaaagc aggacaatcg
     4201 ctaaaaaaag atgaacctct gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc
     4261 agcccgctaa cgggtacagt ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc
     4321 ttgaatgatt cagatgaaat gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa
     4381 gaatttgacg aattataaaa tcagaaaatc acttgaaaaa agttgacact gattcttcca
     4441 gttgctataa tcataagcaa tcctatatga aacgagaaag ctttgaaaag aagagtacat
     4501 gtcacggacg tttgaagaga gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa
     4561 gatggtctta gagcagagta agcgagcgta agcaaactta ctacgggagt gcccgttaca
     4621 gcgcgacagt atacggaaag aagctaagtc caagtactgg aagaggctgt tattgcgaag
     4681 taacggtaaa tcaaggtggt aacacggaag ttcaagcttt cgtccttttg cttaaggcaa
     4741 gagggcgaag gcttttttgt ttgaaaaatc ataagagaaa gatgtgaatg gcatgtcatt
     4801 aatccaacta acgaatgtca aaaagacatt caatgggaaa aacggaaaca tccaagccgt
     4861 caatgacgtt tcactaaacg ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg
     4921 cgcaggaaaa agtacattag tccgtctcct gaatggactt gagcttccca cgagcggcga
     4981 agtgatcgta aacgatcaag atattacaaa attaaaaaac aaagaattac gtacattccg
     5041 aaagaaaatc ggtatgatct tccagcattt caacctgtta tggtcaagaa ctgtacttga
     5101 aaatatccag ctgccgctgg aattagctgg cgtaccaaag agcaaacgta aagaacgggc
     5161 agaagaactg ttgcgtttag tcgggttaga aggacgcgga caagcatatc cttcacaact
     5221 ttccggcggg cagaaacaac gcgtcggtat cgcacgagca ttggcaaatg atcctgagat
     5281 cctgctttgt gatgaggcta ctagcgcatt ggacccgcaa acaacagaag aagtattaga
     5341 cttattgtta gcgatcaata agaaattgaa tttgaccatc gtcttgatca cacatgaaat
     5401 gaatgtgatc cgcaaaatct gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga
     5461 agaaggagac gtattgactg tcttcagaca accgaaaaaa gaagtaacca aacgctttgt
     5521 acaacaagac atcgaaccag aagctgattc gacagaacta ttagctgaac tcatcaaaga
     5581 aaatccaagc ggccgtttgg caactttgac ctttagcgaa agaaatgcca acgagccagt
     5641 catttcccaa gcgattcgac gctataacat cgatgtcaac gttgtgtatg gaaagatcaa
     5701 acaagtgaaa gaaggttcct ttggttcatt gactgtcttg atgactggtg aaaccaaaga
     5761 attagatcaa gcagaagtgt tcatcaaaga acaaggagta ggaattgagg tgatccatcg
     5821 tggataaaag cttttcagaa acatatctag attttaaaca aatcaacccg gaaacgatga
     5881 atcaggctat ccttgataca ctatatatga cattaatctc aatggtctta gtgacgatca
     5941 tcggattagt tttgggattg atcctttact ctattggacg caaaaacaaa ccgtatgctc
     6001 gaatcattta tggaatcgta tcgatcatca gtaatatttt ccggtcaacg ccatttatga
     6061 tcctgatggt tttgatcatt ccatttacta aagcaatcgt aggcactatg ctaggtgcgc
     6121 aagcagccat tccagctctt gttttatccg cagcaccatt ttatgcccgt ctagtagaga
     6181 ttgcttttcg tgaagtcagc cctggtgtac tggaagcagc agatgcaatg ggagcaagtt
     6241 actgggaaat catctggaag gtcttgatac ctgaaagtat tcccgcattg atttctggat
     6301 taacagttac aacgatctcg atgatcggat ttactgcgat ggccggcgcg atcggcgcag
     6361 gtggacttgg aggtcttgct tggcaggaag gctaccagcg tggtaatctg acagtgacat
     6421 tcgttgcgac tttaattatt ttactaatcg tatttatcgt tcaaggaatc ggtgatttct
     6481 taactaagaa aacagacaaa agataatagg atataggggg atgtaggata tgaaaaagaa
     6541 aatttttgga ttagcagcag cgcttttgct gactgtagga ttagcagcat gcggtaacaa
     6601 cagcgacagc aaagattcag caaataaagc agacgacacg acattgaaag tcggcgcttc
     6661 accgacaccg cacgcagaga ttttggaaca tgtgaaacca ttattgaaag atgaaggaat
     6721 cgatcttgaa atcgtcaaat tcgatgacta tgtattaccg aaccaatctt tggaagaagg
     6781 cgacattgat gccaattact tccagcatat tccttatttg aacaaagaaa tcaaagaaaa
     6841 aggatatgac tttgtcaatg ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa
     6901 aaaagtcaaa gatgtttccg aattaaaaga tggtgcaaca atcattactt caaattctga
     6961 atcagactgg ggacgcatca tcacaattct acaagatgct ggcttagtca aagtcaaaga
     7021 tggagtagat ctggaaacag caacattcga tgatattgaa gaaaacccta agaacttgaa
     7081 gttcaaccat acgatcgacc catcactttt agcttctacg taccaaaatg atgaaggtga
     7141 cctagttgca atcaacgcaa acttcgctta cggcgcaggg ttgaatccat tagaagatgc
     7201 cgtattgctt gaaaaggaca actctcctta cgtgaatatt attgcgactc gtaaaggtga
     7261 tgaagacagt gacaaaatca aaaaactgat tgaagtgctt caccgcaaag aagtccaaga
     7321 atggatcaca gacaaatggg gcggctctgt taaaccagta aaggctgatg caaaataagg
     7381 tatggaacaa aaaagatgag aaataagtct gtaatggc
//
LOCUS       DAZKNO010000131         7414 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000131 DAZKNO010000000
VERSION     DAZKNO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.133"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..1018
                     /locus_tag="ACG8MY_002126"
     CDS             161..1018
                     /locus_tag="ACG8MY_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0431296.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1011..1913
                     /locus_tag="ACG8MY_002127"
     CDS             1011..1913
                     /locus_tag="ACG8MY_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0431297.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1946..2296
                     /locus_tag="ACG8MY_002128"
     CDS             1946..2296
                     /locus_tag="ACG8MY_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0431298.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2345..3268
                     /locus_tag="ACG8MY_002129"
     CDS             2345..3268
                     /locus_tag="ACG8MY_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0431299.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTMEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3346..4239
                     /locus_tag="ACG8MY_002130"
     CDS             3346..4239
                     /locus_tag="ACG8MY_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0431300.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4277..4840
                     /gene="amaP"
                     /locus_tag="ACG8MY_002131"
     CDS             4277..4840
                     /gene="amaP"
                     /locus_tag="ACG8MY_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0431301.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4854..5042
                     /locus_tag="ACG8MY_002132"
     CDS             4854..5042
                     /locus_tag="ACG8MY_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0431302.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5055..5957
                     /locus_tag="ACG8MY_002133"
     CDS             5055..5957
                     /locus_tag="ACG8MY_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0431303.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            5989..6231
                     /locus_tag="ACG8MY_002134"
     CDS             5989..6231
                     /locus_tag="ACG8MY_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0431304.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6487..6858)
                     /locus_tag="ACG8MY_002135"
     CDS             complement(6487..6858)
                     /locus_tag="ACG8MY_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431305.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2723 a         1246 c         1463 g         1982 t
ORIGIN      
        1 ccatgaaaaa cgaatagtta gcgcttaccg atgcgttttg tgataaaaaa attttaaaat
       61 attcaatgaa aacaaagcat atattttgct ttaaaaccaa atgaggaata ttctagatgt
      121 agaacaggtt agaaaaagat tagaaaaagg agtgattttg atggagaaga gatacggcgt
      181 agttcctgtg gtaactgctt cagacgaaaa ttatgctcct tacttgagcg tgatgatcgc
      241 aactgcatta gaaaattgca ataaaacaag acgcataaaa ttttatgtga ttgacgatgg
      301 tctatcagaa tatagtaaag aaggattgga agaaacggta aataaatatt caagtaatgc
      361 aagtatccaa ttcctgacag tggaaaaaga tatctacgaa gacttcctag tcagtgacca
      421 tatcacaact accgcttact taagaatttc tttaccaaac ttattagcta aagaggatta
      481 taagaaagtc ctttacttag attcagatgt gttagtacta gacgatatcg taaaactata
      541 tgatgagccg ttaaatggaa aaacaatcgg cgctattatc gatccaggcc aagtgaaagc
      601 tttggaaaga ttaggaatcg attccgatga cttgtacttc aattcaggag tgatggtgat
      661 tgacattgat caatggaata aaaaagaaat tacagaaaaa actatccatt acctcagtga
      721 aaatggcgat cgaatcatct atcacgacca agatgctttg aatgctgtac tttatgaaga
      781 ttgggaacaa cttcatccaa aatggaacat gcagacttcg ctgatttttg aacgtcatcc
      841 agcaccaaat gaaaaatatg aacgtcagta taaagaagga aatgaaaaac cttcgatcgt
      901 tcactttaca ggacatgata aaccttggaa tacgttgaaa gatcatccgt ataccaattt
      961 atacttaaaa aaattagcac acagtactct aacgaaagta ggcgagatca atgaataaaa
     1021 aagaaatagc agtagtcgca agttgtaaca caaaattcgt accacattta gctgcattat
     1081 ttgtctcagt attagataat tgcaacccgt ctaaatttgt tcggttctat gtgattgacg
     1141 atgatatcga ttttgaaagt aaacagcttt tacgcttctc agtgaaaaac gcacgaatga
     1201 actcagatgt ggaattcttg aaaatcaata aagaattctt tacaaatgtg gtaatcagcg
     1261 atcgtattcc agaaacagct tattatcgta ttgcgattcc ggaattgttc cgcggcacag
     1321 aagttgaacg tatcttatat atggattgcg acatgatcgc tttacaagat atctctaaat
     1381 tatggcgtct tgacttcggt gattcaatcg tagcagctgt tgaagacgct gggttccatc
     1441 aacgactgga aaaaatgaaa atttcagcta aatcaatgcg ttatttcaac tcaggtttga
     1501 tgctgatcaa tgtgaaaaaa tggttagatg aaaatattac gcaaaaagta ttagatttca
     1561 tcgaacataa tccagaaaaa ttacgattcc atgaccaaga tgcattaaat gctatcttac
     1621 atgaccgttg gttaccactt catccgcgtt ggaatgcaca aggctatatc atggcaaaag
     1681 caaaaaaaca tccaacagca gcaggtgaac gtgaatacga agaaactcgc aacaatcctt
     1741 acattatcca tttttcagga catgtgaaac cgtggagcaa ggattttgaa gggccaacga
     1801 agaaatatta cgaaaaatat gcagggatga cggctttccg ttgtgtagct aaattcccta
     1861 aatatccaaa atatgcaaaa gttcaaccaa aaacagatgt agttgttaaa taatagaaga
     1921 caacaactga agagggggag taggaatgga aattacaatg tctaaaaatg cagtagagac
     1981 gttaattgaa aaggttgggg aaaatacaaa aatcgcactc gcgcttatca atgattccga
     2041 tccatttcta agggacaaag gtgcttttgc aaaaggcagc ttttttcaaa tcattccttt
     2101 tgtttcggaa tttggagaat atgcgactaa aatcgagcat ccattattgg acatctatac
     2161 atctaaatta gagcaaaatt atttcggtaa acgattgaac atggacttca acaaacagct
     2221 tgacagcttt tcattagaaa atgaaatcgc agtactcgat tataatatca aattaaagaa
     2281 ctgcttcttc agctaatagt ttcgagaaag tccaaaaagg aggtatacat tgagatacta
     2341 ctttgtggat gaagaaaaga atgtaataga aacagatgaa aaaaaatata attggctcgt
     2401 acttgatagt gcaaagcccg aagagattga gcaagcaatc gaagaattcg agcttcctga
     2461 tgatattttc gtaggaaccg aatatccgga agaagtttct cggatggagc gcctttatga
     2521 gacgaaacta gagcatccat tttctttggt agtgattaat ctagatcagt cagataatcg
     2581 tatcgaaaaa aaattaacgc caatatcttt tgtactttcc gataatctct tgattacttg
     2641 tatcgataga aaaaccgaat ttgtggatcg gctgctgaca aagcatggaa atcatttaaa
     2701 ctcgtttgaa aagatcatca cgtatacatt attagatatt tacacccatt acgtgaaaga
     2761 actaagagag atgaaaaagc gaattgacgc tttggataaa gcagctcgta aaacaatgga
     2821 aaatgaagaa ctgtataaac aggcagatct tgaaagagac atcgtatata tcgatcatac
     2881 cttaagagat caacgggaaa caatggatca attatggaat tctgaaactt tcatcaaacg
     2941 tttagacgat gaacagctac tctatgatgt ccatttgaga cagcgacaaa cagaaaaaat
     3001 gatcgagatc tatcgagatt tattagagag tattggtgat ctattcaacg ggatgatgga
     3061 caataatcta aatcatatca tgaaatatct agattcagct gcattaatca tttccgttcc
     3121 ggcgttgttc gcagggattt ggggaatgaa tacaggagga ttaccgggta aaagttctgc
     3181 tctaggcttt atactcgtag tctttggctc tttgatagca gcagtagttt taggttacca
     3241 tttataccgg aaagattata caaattaaag aatctattta aaacaggtat caaacaaaga
     3301 aatttgtttt ggcaatacaa tttaaaaact aggaggaata cagcaatgac agaaccaaaa
     3361 ttaatcgacc cgcgaaactt gtaccatacg gagaaatttc ctaaacaaga tcaggataca
     3421 ccagctttac agcagaatat gattcctgtt cctgattgtg gagaagaatc ctatgaaggc
     3481 aataatcaac tagaaaaccg ccgtgtacta atcactggtg gagattcagg gatcggtcgt
     3541 gcagcagcga ttgcatttgc tagagaaggg gcagatatcg ctcttcaatt cttcccagga
     3601 gaagaaaaag atgcagagga agtagcaaaa tacgtgaaag atgcaggacg taagattgtc
     3661 ttactacctt ttgatcttcg tgacgaacaa gcacctaaag aaatcataga taaagccgtg
     3721 agtgaacttg gcgggctaga tacgatggta ctaaacgcag gacagcaaat ttcttgtcag
     3781 tctattgctg agttaccact tgagcaagtc aaagacacat ttatgataaa catcatcgct
     3841 atgtttgctt tagtgaaaga agctgttccg catatcccag caggaggagc aatcgtaaca
     3901 acaacttctg ttcaagcatt caatccaagc gaacatttat tagattacgc agcaacaaaa
     3961 gcttcaatcg ctaactttac gatcggttta gcaaaacagc ttgcacctaa aggaatccgt
     4021 gtaaatggtg tagcacctgg tccaatctgg acaccattac aactagacga aggacaacct
     4081 tctgattcga tccctgaatt tggtcagcaa agtttactag aacgtgcagg tcagccagga
     4141 gaattagcac cagtttatgt tttcttagca tcgaataaag caagttatgt tacagcccaa
     4201 atttacggag taactggtgg agaagcgatc aacttataag aagttgatcg tttcacccct
     4261 atgaggaggt gcgaaaatga ataaaggtgt aaaaacgatc ggagtgatcg gaacattact
     4321 tttgctatct gtgcttttgt ttgcgatact attaagtgca acctatccat tgccttttca
     4381 gctagagaag tttcgattct tttccatcac taattcatac gtgcaacaat atgtattttg
     4441 gcttgccgtt gcatttgcta ttttgacaat tatcacgtta cttgttttcc tgttttatcc
     4501 aaaaacaatc ggaaactttc tactgaaaca tggtgatggg gagttgacgt tggataaaaa
     4561 agcaattgaa ggaatggttc gttctcactt gcatgaagat gaattcatcc attcaccaaa
     4621 agtaaatgtc aaagcaacga aaaacaagat cgccgtcaat gtaaaagggg agttgaaacg
     4681 aacttcagca ctcattggta aaacaggaac attgatgaaa gaaatcgaaa atgaagtaca
     4741 acacatattg ggaacaaaag agccagtgaa agtatcggtg aaatacacag gctttgaaga
     4801 accagctaaa cgaagcaatg accgcaaacg agtagaatag ggggggacca aaaatgaaag
     4861 aactattttc gacatacaag ctccctatca tctttggggt acttggattg atattagccg
     4921 ttttatggat caccgtgggc tttttcaaga ccttattgat cttgatcatg gcagcgatcg
     4981 gcgtcgctat cggtttttat ctgaaacaaa cgaaaatctt agatcactat tttaaatctt
     5041 aggaggaaac atccatgaat aacaataaca aaaataataa caataaaatg aacaaccaaa
     5101 aaccagtgtc tgcacatcca gacccaacga caccaaacca atcaaatggt gtgaacgatg
     5161 aaaagaacaa aacagcacca gtatctgcac atccagatcc agtagtccct aacgttcaac
     5221 aaggagtaga agagaagaaa catgatgcag aaaagcatgc acctcatcat ccaggaactc
     5281 cagaatctaa agttccagaa caacatgatg caatcgatcc ttctaaacct gtggaagagc
     5341 aaaaacatcc ttctgaccat gcgaaagcac aagaaaaact tagttctgac cacacaaacg
     5401 gacaaggaaa acttggttct gatcatacaa ataaccaagg aaagcatgat tctgaacagg
     5461 aagattcaat gcgcggtgag ctgaattatg aagataaagt tgtgcaaaaa atcatcggaa
     5521 tcgcaattga acaagtagac ggtttattat ctgcaaacgg tggtttcttc tctaatgtgg
     5581 caggaaaact tgtgaataca gataatgtaa cggctgggat cgaaacagaa gtcggtaaaa
     5641 aacaagtagc agttgatatg gatgtcattg tggaatatgg caaagatatc gaaaaaatct
     5701 ttgaagaaat gcaagaagta atcggcaaag aagtaaaaaa tatgacacat ttagaagtga
     5761 tcgaagtcaa cgctaacgta gtggacatca aaacaaaaga agaatttgaa aaagatcaag
     5821 aaacggtgca agacaaagta actgacgcag cgaaaaaaac cggtgaattt gcttcgaatc
     5881 aaacagataa agtaaaatca gcagctggaa gcggcgcaca aaaagtaaaa gaaaatacag
     5941 aaccacgggt tcagtaataa ggaagtaaaa cattaggagg aacgaaacat gggatttatt
     6001 tggtcactta tcgtcggagg aattatcgga gcaattgcag gagcaatcac gaataaaggt
     6061 tcttcgatgg ggattattgc aaatgtcgta gcaggtttag ttggatcagc acttggtcaa
     6121 gcattattag gttcttgggg accaagtatt gcaggaatgg caatcgtgcc atcattgatt
     6181 ggtgccatca ttttagtact aatcgtatca ttctttgctc gaaaactgta aaaagacttt
     6241 taaaaagagg tcgtgaaaaa gctgttcttc atcaagtaat aagaacagcc aaacaaaaat
     6301 agcttctcat atttttcatt tggttgggca tattatcgaa gatgcaagct tttgaacact
     6361 gtttatacgg aataagaggc tgtgacaaga cttttgtcac agcctcttat ttatgcgggc
     6421 aaattcccaa ttagattttt aaaagttttt ccttaaatgt gtctttattg atacagagaa
     6481 gcttctttaa taaaacaatg cctccctaaa ttttcttgag ataggaaagg tgaacataca
     6541 aaataatacg accactttga aaacccagat gatccacaaa tggatcgtat gatactgatg
     6601 aagtaaaaag caatggatca gaaactcaaa gagattgaaa aagacaagga agaaataata
     6661 tctctttttt actcttgtct gttttagcca ataatagctc gtaaagtaat ctagacaaac
     6721 aaatagaagt aaacaaagtt tcgtaagagc agcaatcgta tcatgttgct ctgctagacg
     6781 attgacgagc aggaaaaatg ttggtaatgg aatatagatc acgagtaatg aaagaaaaaa
     6841 gtaaacaata aatggcatac tattcctcct aattgtgtga gttcgccctc attttaaaat
     6901 aacgatattt gtttgtgaag ctttttcgtg tcgtaacaaa tgttatctaa tgtattttta
     6961 aataaataat cattattatc aaaataatgt taaaaataaa aaagaattgt ccatcactgt
     7021 tattcaaatc attttatcaa aactacatag taaatgttaa aaagcaacat gacataatat
     7081 atagaaggct ttacagtata tatgggagct tactaaaaaa gaatggagta agagttaaca
     7141 caatgggcca ttagctgaag ttttaagcac ttcaaggtat gaaacagaat ggcctgtaat
     7201 actacatgat tatttagact gctcaaactg ctatcagtct ggtggaatga ataaagtatt
     7261 atactaaaag cagatccaac tacaacgtaa caggaaacca gttgtatcag gaaaagtgat
     7321 agtacatgga gaagaaaagg ccccctttgt atgagtcagt acatatctta tttatagata
     7381 aaacggtatt caatcgaaat aaaaaatctg aata
//
LOCUS       DAZKNO010000132         7378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000132 DAZKNO010000000
VERSION     DAZKNO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.134"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..936
                     /locus_tag="ACG8MY_002136"
     CDS             <1..936
                     /locus_tag="ACG8MY_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431306.1"
                     /translation="AYPLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSG
                     VLLYENIYRECAPENWYRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIA
                     SALAYSAVIHGSRSVLVYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFS
                     TFVETFTNSTGEYAGSAESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGH
                     LSDIGFFYTITQYGVGILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTG
                     INIDWFYPIFTPAIPVCLAVIEYIYQECRSVANIE"
     gene            961..2070
                     /locus_tag="ACG8MY_002137"
     CDS             961..2070
                     /locus_tag="ACG8MY_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WavE lipopolysaccharide synthesis family
                     protein"
                     /protein_id="HFQ0431307.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            2112..3446
                     /gene="gmhB"
                     /locus_tag="ACG8MY_002138"
     CDS             2112..3446
                     /gene="gmhB"
                     /locus_tag="ACG8MY_002138"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFQ0431308.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            3481..5118
                     /locus_tag="ACG8MY_002139"
     CDS             3481..5118
                     /locus_tag="ACG8MY_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFQ0431309.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            5220..5615
                     /locus_tag="ACG8MY_002140"
     CDS             5220..5615
                     /locus_tag="ACG8MY_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFQ0431310.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            5633..6364
                     /locus_tag="ACG8MY_002141"
     CDS             5633..6364
                     /locus_tag="ACG8MY_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0431311.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            6388..>7378
                     /locus_tag="ACG8MY_002142"
     CDS             6388..>7378
                     /locus_tag="ACG8MY_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFQ0431312.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKY"
BASE COUNT         2414 a          962 c         1523 g         2479 t
ORIGIN      
        1 gcgtatccat tgttctgtac tttaattaac caagcgtgga atttggattc aatgctaaat
       61 acgatatgtg gtgtaacatt tctttcatac attcttagaa catctatttc acttattcag
      121 aaattttcag gagtacttct ctatgaaaac atatatcgtg agtgtgcccc ggaaaattgg
      181 tatcgtggag gctttttaag aattaatcca ccatgtttta caattataat aataccgatt
      241 gctttgtata tgattgagcg acaagttgta gctagaagaa aagtaaaata ttatttactg
      301 attgctagcg cactggcata ttctgctgtt attcatggtt ctagatctgt ccttgtctat
      361 caaattattg tacttggttg ctatatcctt tttaagaaag gttcttcgaa aagaaaaatt
      421 gcaatgtggg ttttagctgg gttgcttgta gtcatattta ttaattcgac catgttctca
      481 acatttgttg aaactttcac aaacagtaca ggtgaatatg caggatctgc tgaatctcga
      541 tttgcttcag tttggttttt tattcctaaa tttttgcaat cgcctttgtt cggtactggt
      601 attcttactg gtgaggaatt tacgtttata ttaccaggag gagttagagg acacctatct
      661 gatattggat ttttctatac aattactcaa tatggtgtag gtatacttat atttgatatt
      721 ctgtttatta taaaagggtt caaaacagca ttacttgcat caaaagtggg tatgactggc
      781 tatcaatatt tagcatttgg aattacatta aatgtcttgc taacagggat taatatagac
      841 tggttttatc cgatatttac tccagctatt cctgtatgct tagcggttat agaatacatc
      901 tatcaagaat gtagaagtgt ggctaacata gaataggaaa tattaatagg agtgcatctc
      961 atgaataaga aagagatttt atataacata ttgaagaagt acgaaaattt accatatgtg
     1021 tttaggaaca tgtttggtca taatataagt aaactcgtat cttggaaatt gactgctgat
     1081 tgtgcaacag tagaagaaaa ttctgaagtt tatgcagcgt ttcaattgcg ctcaaaggac
     1141 atgtctgatg ttccagtgat gggtttagtt aattgttgca aagatgtagc aattgtaatg
     1201 caggggccac tatgtttaaa ggatgatttc tcttataata ctctggccta ctataagaaa
     1261 tgttatccaa atgctttaat tattctttcg acatggaagg acgaagatat taaggcattg
     1321 aaaaggatag aggatcttgg agttatagtt atttgtagtg agaaacctga aaagcctgga
     1381 cacttaaata ttaattatca agttggaaac actttgcctg gaatagtcag agcaaagcag
     1441 cttggtgcaa agtatgtttg taaaactaga acagatcaaa gaatatacca tccaaatgca
     1501 atggcgtttt tctgcagttt acttgaacag tatcctgtta ataacgaaga tgattggaaa
     1561 ttaatacaaa atcagcgatt actgttacta tcaatgccgt atggagacat gtttttcccg
     1621 tattgtttgt cagacttcct gtattttggt gatgttgatg atatgataaa cctcttctcg
     1681 attcctagtg atgtgagaga aaagggagct gtgtctagag gagtctcaag aagggagata
     1741 tctgaaaata atttagctcc agaagttcag cttcttcgaa gctatatttc acgaatgggg
     1801 ggcaatgagg agtgctctat tagggcatat tgggagtttg taaaaaacca tgttattact
     1861 attaacaaaa atcaaattga tttgtactgg cataaatatg taggtcgtta ttcaaataat
     1921 actatttatg gtacgtatta tattgatgat agccaagatg cacttcactg ctataatttt
     1981 gattttataa attggttgaa tttgtacact ggaaaatacg aatacaaagc agaatatgaa
     2041 aaatatatgg actttgtttg ggaagagtaa ttaactttta tagtaaaaag tactttaggg
     2101 aagggcatat tatgaaaaca gtaattatgg cgggcggacg aggaagccga atttcggaat
     2161 tgtttccgga tattcctaag ccaatgattc ctatagatgg cattccagtt ttagaacgag
     2221 aattgtgttc gcttagagat caaggcttta atgatattat tttgactgtt tcctatttgc
     2281 atgaaaaaat agaggagcat ttcggagatg gttcaaaatt aggaattaaa atcaaatatt
     2341 ttattgaaaa aactccactt ggcaatgcag gtgcattgta ttttttgttg gatgaactgg
     2401 gtgatgaacc gtttttactt ttgaatgctg atgcagtatt tgatgttgat ttcaggcaga
     2461 tgattagatt tcatgttgag aataacgcag atgttacttt gtttacgcat cctaactcac
     2521 atccgtatga tagtggatta attatatccg atgacaataa gaaagtgctt caatggttag
     2581 cgaaggaaga tcaaagacct gaatattata aaaaccgagt aaatgcaggt cttcatgtta
     2641 taaatccgaa ggtgttgaga gaaaatccat taatggaaat tgtaaataaa tcaatagttg
     2701 aaggtggggg gctaccatac aaaattgatt tagatagaat gattctgaaa ccattggcta
     2761 acacagggcg tatgtattgc tacgattctc cagagtatgt taaagatatg ggaacaccgg
     2821 accgatatag agctgtaatt aaggactttc aaaagggaat tattgttggt aaaaatctat
     2881 ccaatccaca aaaagcaatc tttttagata gagatggaac gattaataaa tacgttggtt
     2941 ttttgagaaa tgcagaagaa tttgagttaa ttgatggagt atcggaggca attaaaatta
     3001 ttaattctct aggttttctg tgtattgtta taacaaatca gcctgtaatt gcaagaggtg
     3061 aagttactgt tgatgagctt aatagtattc acaataagct tgaaacaatg ctagggcatg
     3121 ctggtggatt tttaaacggt atttattact gtccgcacca tccccataaa ggatatgcag
     3181 gagaggttat agaattaaaa attgattgca attgtcgcaa gccaaaacct ggactgttat
     3241 tagaagcatc aaaggatttt aacataaacc taagtcaatc atttatggtt ggagatagtg
     3301 aaagtgatgt aattgctgga aaaaatgctg gttgtaagac agtactgatt acagaagaca
     3361 tcaccttgcc tgctaattat ggccaagact atagtgtctg ttcattgtta gaatttgtta
     3421 aaaatcattt atgcactatg caataattgg tttagtgaaa cttgtaaaag gagtaattag
     3481 atgaaggtat taattccagc agcaaaagtt gttgaaaaag aattgcagag tataggaatg
     3541 ttaccagcaa ttatctatcc aattaataaa aaaattgtct ttgattattt gtataatcag
     3601 tataatgcaa tctgcgattc attggatatt atctgttatg aaaaagcgga taaggttcat
     3661 agaagactga aaaactatca tgattccaga attcatatat gtgaccttcc tgaattaggg
     3721 gatctagggc aaaccgtgta ttttgggtta tcagatgaag atgagcctgt tctaattaac
     3781 ttcgcagata caattgttat ggagagtatg tctgatatag aaatggatac attctattat
     3841 tctgaagatt ttgtgtcgag tacttggaca tactttaaaa ttaatgaagg aataattact
     3901 gatatccacg ataaagagga agcttacgta tccgataaac gtgaaaaatt atttattggc
     3961 gtatttatgt tttcttcgtc aaaacttctg aagagttgct taagagaggc agttgagagt
     4021 cggaaacagg ggggaataaa ctcattttat cgggctttac agctatacag cgaacgttgt
     4081 ccgctaaagg ccgtaagaac agaaaagtgg tttgatattg gacacatcga taaatattac
     4141 aatacaaagt tggaagttaa agcacgagag tttaatcata tctctataga taaagatcga
     4201 gggatattga agaaaactag cgatgacaag gataaattta ttggagaaat aaagtggtat
     4261 ttaaaacttc ctgctgatat tgaatatgta agacctagaa tttttgatta ttctacatct
     4321 tacgtagctc cctatgttga tatggaatat tatgcatatc atacggttca tgagttatac
     4381 ttatatggtg atttaaaata taaccagtgg catgatctgt ttagccgtat aaagtttatc
     4441 tgtagcgatt ttaagcgata caccttaaaa gatgaaaaga ttagagagtc attggaagat
     4501 atgtatttgc gaaaaaccat gcagagattt caaaaattgt ctgaagataa aagattttca
     4561 gctttttttg agaaacaaat tcaaattaat ggtcgtaagt ttatttcttt aaccggtgta
     4621 attgaacttc taaagaatct tatcccaagt atgctctatg atgtaaatga attttgtatt
     4681 atacatggag atttgtgttt tgctaatatg atggtagatt ccaattgttc ctttgttaag
     4741 gttattgatc ctaggggaaa atttggaaaa tatgatattt atggggacag cagatatgaa
     4801 ttagcaaaac tatttcatag tttagatggt aaatatgatt atatcatcaa ggatctattt
     4861 actgtagaat atgataccga gtgtgcagtg atagattata caattaatac gcgtgtaaga
     4921 gattacgatt tatttgaatt gtttaagtcg gtcttttcgg acgagattaa ggacgatttt
     4981 gataagattg tattgataga agcgttgcta tttcttagta tgattccatt acacggtgaa
     5041 agttttgatc accaaatagt gatgcttgct acaggtatgg aacttcttgc aagagtaagt
     5101 aataaagtta tactctaata taaattgtgc cttaatgaat aacggttaca atactgtttt
     5161 agcaatgtag gcattaaggc acgattatat taattaatac aatagaaggg aagagaaaaa
     5221 tgtatagtga atattctttt gtgtttgata tcgatggcac tttgtgtccg ataaagaaga
     5281 aggatgagcg atatgaggat ttggtgccgt atgagaatat ggttgaaaaa ctgagattct
     5341 ataaggagaa tggtgctaaa attacattgt tcacctcacg caatgttaat tcctataaag
     5401 gaaatttggg aatgataaat gctaatactg caaaagtgct cttggcgtgg ttggataaat
     5461 gggacattcc gtatgatgaa atcctgtatg gtaagccttg gccggggcat aaaggcttct
     5521 atgtagatga tcgaacggtt agacctgatg agtttttgag atgctctgta gatgaattaa
     5581 atgaaatctg caacagttca aaaaaaagat tctaaataag gaggttagtt ctatggaaaa
     5641 aaagatgaca gttatcatta cgatggcagg tttgggaagt cgttttcgaa aaattggtta
     5701 tgcggttcct aaatatatga ttaatgccaa aggaagaact ttatttgatt gggccatgga
     5761 tagtttggtt ggatacaata aaaatgttga taagtatatt tttgttgttc gtcaggaaga
     5821 caacgcttca gcttttataa aggagcattg tgcttacttt ggtattgaaa acgtagaagt
     5881 gattgaaata gactatatga cagatggtca agcaactaca tgtatgttgg ctattcctta
     5941 ttgcaatcaa gacaatgaga ttatggttta caatattgat acatacgtgg aatcaaatga
     6001 gatgaaatac gaagatattt gtggagatgg acatattcca tgctttcatg ctgatggcga
     6061 tcattggagt tttgcaaagc ttaacgatcg aaatgaagta atagaagtcc gcgaaaaagt
     6121 tcgcatttct gataattgta ctcttggtgc gtattatttc tcatcggcaa agctgtataa
     6181 agctttatat gaagagtatt atgcagactc ctctaatatg gagaagaatg aaaaatatat
     6241 agcaccatta tataacttta tgataaaaaa agggttaaag gtgactatta gcatagtaga
     6301 tgcgaaaaag gttcatgtgc ttggtactcc agaagagctc aatgtgtttt tgaatcaaga
     6361 ataaatagtg tttaggagaa aatagcagtg accagtaata atctaattgt aaaaacatta
     6421 agcagatata agaatactcc acttcctgtt aaggctgcat tatggtttat ggtatgtggt
     6481 tttttacaga gaggggtttc aatgattaca accccaattt ttactagagt tttgtctaca
     6541 gaacaatacg gtttatacag cacttatcta tcgtgggaat ccttattgct catgattaca
     6601 tcgcttagtt tgttcaaagc catgatgaat ctttatgtaa aatatgataa tcaagaacat
     6661 attctttcga cagtatccgg attgacttta gtactgacta ttttttggtt tgttattgct
     6721 atttttatta aggatatatt ggcagaaatt ttaggattga caccagttct agttgtctgt
     6781 atgttctcat attttatatt tcaggcagaa atacaatgtt ggtctttaca caaacgatat
     6841 atatatgatt ataagacttt attagtagct acccttgcag ttactgttgg ctcatctgta
     6901 gttggctgtt tagcggttct gtttatatct cctaccgctg aggcgagagc cttaatgaat
     6961 gtcctagtga ctggtgttgt tggcgtggct gtgtatatat cggtatttag aagatgtaag
     7021 gattttaagg ataatgaagt gtggaatttc gcagtagtat tttgtgtatc gttgatgcca
     7081 cactatttat ctgaatttat tctgaatggt tctgataaaa ttatgataaa ttacttatgt
     7141 ggttcgtcag atgttgctat ttacagcgtg gcatattcta ttggtgctct aattaacctt
     7201 gttacaaatt ctgtcaattc cacttttgca ccttatcagt atcaaaaaat caagtcagga
     7261 gaatatgatt tactatcaaa aagagctaat gaagttcttg gtttggtagc ggctatgctt
     7321 tttgcattaa tgctatttag taaagaaatt gtgttgatat ttggtgggca gaaatata
//
LOCUS       DAZKNO010000133         7255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000133 DAZKNO010000000
VERSION     DAZKNO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.135"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(273..1619)
                     /gene="gor"
                     /locus_tag="ACG8MY_002143"
     CDS             complement(273..1619)
                     /gene="gor"
                     /locus_tag="ACG8MY_002143"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0431313.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1753..3006)
                     /locus_tag="ACG8MY_002144"
     CDS             complement(1753..3006)
                     /locus_tag="ACG8MY_002144"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0431314.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3012..3935)
                     /locus_tag="ACG8MY_002145"
     CDS             complement(3012..3935)
                     /locus_tag="ACG8MY_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0431315.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4156..6648)
                     /locus_tag="ACG8MY_002146"
     CDS             complement(4156..6648)
                     /locus_tag="ACG8MY_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0431316.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6655..7122)
                     /locus_tag="ACG8MY_002147"
     CDS             complement(6655..7122)
                     /locus_tag="ACG8MY_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0431317.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         1996 a         1589 c         1164 g         2506 t
ORIGIN      
        1 ttaaacatct gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga
       61 acttaagagt gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa
      121 atctccaact tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa
      181 ctaaaaaaca aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc
      241 aataaaaagc tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct
      301 cctgttggat ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata
      361 gccacggcaa aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg
      421 attctttctt cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt
      481 aatgcaaagt acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat
      541 ttttccaatg cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg
      601 actgtcggaa caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct
      661 gataaacgtc taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca
      721 ccaacagcaa aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct
      781 tttttgtcca attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt
      841 ccaccggcaa aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta
      901 tattcgtcat tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca
      961 gcttcttggt acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt
     1021 cgttcttttc gatatgccca catgacttca ctacctaaac cacgaaatac acctgaaagt
     1081 tcagccgcaa tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca
     1141 aaaaatccat ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg
     1201 cctccagtag cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt
     1261 tgactatcga taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct
     1321 ctttgataag caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg
     1381 aaatcaacat tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt
     1441 tccaaaatcg tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat
     1501 gttccgccca attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg
     1561 tttgctgaag caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt
     1621 ttgttcctcc ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag
     1681 aaaacaagtg taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata
     1741 tcagcttttt ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac
     1801 tggcatagtt aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt
     1861 atgcataact tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc
     1921 atctcctaca gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata
     1981 agataatact tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg
     2041 ttcgttttca gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt
     2101 ccataactcg tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact
     2161 gtctacagcc gctttataga catttgacac cgtagataca taatggatcg atttcattct
     2221 tccttcgatc ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat
     2281 tgacatatcc actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact
     2341 gtttcgttct tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga
     2401 acatccgcca cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga
     2461 ataagagata cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg
     2521 aatctctgcc acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc
     2581 ttctttccag aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat
     2641 cggcagacct ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac
     2701 tgcagaaata ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc
     2761 ttcatgggtg accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc
     2821 aactccttca gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc
     2881 attcccgcca atatatactg catctgctcc ataatggatc gcggttttta acttttccag
     2941 tgttcctgct ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt
     3001 catcatactc cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca
     3061 cggttttcag gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc
     3121 cagctacctt ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat
     3181 ttttcgcctg gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt
     3241 tcattcaatt cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta
     3301 tcttcataaa tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt
     3361 tcacgatctt tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt
     3421 ttggattgat ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt
     3481 tcttccatcg ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg
     3541 ccttttttcg cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa
     3601 atatacggta tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca
     3661 actgtgatca catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc
     3721 attttttcag gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt
     3781 gctaattcta ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca
     3841 aattgctctt caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt
     3901 tcaatagttt ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata
     3961 atagtagcat agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa
     4021 aattatttca caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga
     4081 ttgtgacaaa aatcttgtca caacctctta atcaagtctt actattataa aaattcagct
     4141 acacaggcca agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa
     4201 tgtgatctta ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc
     4261 aagcaaagcc tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg
     4321 tgcaccatat tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat
     4381 tttcacttga atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac
     4441 gattgtatgg atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt
     4501 caagaattca ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt
     4561 gtgatcttta gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt
     4621 cgctccgata tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt
     4681 agaatctgtt aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg
     4741 tgctttctcc acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt
     4801 caactgtcca ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt
     4861 actgtatttt tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa
     4921 catgacttct gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa
     4981 catgaatgaa ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc
     5041 acgtgaaact gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc
     5101 cagttctaac aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca
     5161 ctccgataca actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg
     5221 ttttgcttca attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg
     5281 taattgagca gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag
     5341 ttcatcgatt tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg
     5401 gactttcgct gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt
     5461 gatataacga acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc
     5521 atggtgctct tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt
     5581 tggctcatct acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata
     5641 tttttgatat tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc
     5701 cggtttcaag atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt
     5761 atgcaattca tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt
     5821 tttcattcga tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc
     5881 aagcatcatc aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg
     5941 agctaaccct tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt
     6001 attctttgtc cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac
     6061 aactgggtcc agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag
     6121 cgtaggtgtt ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt
     6181 cattcctttt ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc
     6241 ttttgaaagg ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt
     6301 taataagctc atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc
     6361 gctgctagct aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt
     6421 agggtaagaa cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt
     6481 ttcattcaaa tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa
     6541 ggctagtaaa acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg
     6601 tgcaatttct aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt
     6661 cccatcttca taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc
     6721 ttcatattca ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc
     6781 tttttttgta attatttggt cttcataaag tttttgaata atagcaaaag catttttctc
     6841 actgattgta tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat
     6901 ccttactttt gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc
     6961 acgttggatg gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa
     7021 gaggtctgcc atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt
     7081 tatatatgat tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga
     7141 ctaatattga ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt
     7201 taaataaatc ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatc
//
LOCUS       DAZKNO010000134         7252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000134 DAZKNO010000000
VERSION     DAZKNO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.136"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG8MY_002148"
     CDS             99..746
                     /locus_tag="ACG8MY_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0431318.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG8MY_002149"
     CDS             1139..3784
                     /locus_tag="ACG8MY_002149"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0431319.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG8MY_002150"
     CDS             4094..5407
                     /locus_tag="ACG8MY_002150"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0431320.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG8MY_002151"
     CDS             5397..6050
                     /locus_tag="ACG8MY_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0431321.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG8MY_002152"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG8MY_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0431322.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG8MY_002153"
     CDS             6947..7147
                     /locus_tag="ACG8MY_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0431323.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2396 a         1229 c         1593 g         2034 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt at
//
LOCUS       DAZKNO010000135         7225 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000135 DAZKNO010000000
VERSION     DAZKNO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.137"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..225
                     /gene="mobC"
                     /locus_tag="ACG8MY_002154"
     CDS             <1..225
                     /gene="mobC"
                     /locus_tag="ACG8MY_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFQ0431324.1"
                     /translation="KAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQ
                     HDVAPGDAQRLSENLKIMQKELQKIWQQLK"
     gene            207..1121
                     /locus_tag="ACG8MY_002155"
     CDS             207..1121
                     /locus_tag="ACG8MY_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0431325.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            1081..1359
                     /locus_tag="ACG8MY_002156"
     CDS             1081..1359
                     /locus_tag="ACG8MY_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431326.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
     gene            1361..2050
                     /locus_tag="ACG8MY_002157"
     CDS             1361..2050
                     /locus_tag="ACG8MY_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431327.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(2170..2256)
                     /locus_tag="ACG8MY_002158"
     CDS             complement(2170..2256)
                     /locus_tag="ACG8MY_002158"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0431328.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            2902..3834
                     /locus_tag="ACG8MY_002159"
     CDS             2902..3834
                     /locus_tag="ACG8MY_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0431329.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            3851..4321
                     /locus_tag="ACG8MY_002160"
     CDS             3851..4321
                     /locus_tag="ACG8MY_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0431330.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            4405..4857
                     /locus_tag="ACG8MY_002161"
     CDS             4405..4857
                     /locus_tag="ACG8MY_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0431331.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFS
                     HSIATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
     gene            4984..6165
                     /locus_tag="ACG8MY_002162"
     CDS             4984..6165
                     /locus_tag="ACG8MY_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0431332.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            6255..6524
                     /locus_tag="ACG8MY_002163"
     CDS             6255..6524
                     /locus_tag="ACG8MY_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="HFQ0431333.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYK
                     KYPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWNASSRTAKYYYYI"
BASE COUNT         2675 a         1146 c         1200 g         2204 t
ORIGIN      
        1 aaagccaagg cacaaaacgc gcgtctggtg acaccgaaag tggcgccaga cattgcccag
       61 gcaatcgcgc gcgatttagc caaagctgga gggaatatca atcaaattgc taagtggtgt
      121 aatacacacc aacacgacgt tgctcctgga gacgcccaac gcttatccga aaacctaaaa
      181 atcatgcaaa aggagctaca aaagatatgg caacagttaa agtaagccga accacttctt
      241 gtagtcgtgc aattaattac gctgaaccac gagcaaccat taaaacagga ataaactgtg
      301 acgtcgatta tgccaaaagt gaaatgaaac aaattcgcat gctgtatgga aaagacgacc
      361 gtgtacaggc acacttattg attcaatctt ttcgaccagg agaaattacc gctgaaaaag
      421 ctaatcaact aggggcagag tatgcaaaaa aaatagcccc cgatcaccaa atagctattt
      481 acacacatac agacaaagac catattcata atcacattgt cattaactcc gttaatctgg
      541 agactggaaa aaaatttcaa gctcatggtc aagcatttct aaacaaatgt tatgacctaa
      601 atgacgagat ttgtcttgca cataacttac caatcactga gcgaggaaag aaaccagaaa
      661 aacgaaccat gtctgaaata aaattaaaag aaaaaaatga acctgtttgg aaagacgaaa
      721 ttcggattgc gatcgatcaa acaatgaaaa acaaaaaaac cagaacctat gaccaatttt
      781 gtaattccct caaaacattg gggattcatt gcttcaatcg cgggaaaaat ttcacttacg
      841 aattgatcca caaaaagaaa aaagtacgat ccaacagact aggaaaggac tacgaaaagg
      901 agacgatttt acatgagctg gacagacgag aacgaaccta taaccaacag cgaaccaacg
      961 agctacgaag agctcgagct agcattcgcg aaactgcaga agaagtacga agagaacaac
     1021 gaactcttac gcctgaacaa ccggaaatta gaccggctcg aagaaaccat gaacaacacg
     1081 ttgaaagagg aacagaccac caacaagaat tatcgctcta aaacgttaaa agacatagaa
     1141 aacgaaataa agaaacaacg tgcttttctc gttccttata cgcaagaaag gatacaaact
     1201 acgattgatt tacaagttaa aaaactccat caagagctat tagaccagcg aaataaggaa
     1261 cagaaaaagg tgcttattca acgatcattg gattttcttg cccccttatg ttgcttagtc
     1321 ctgacactgt tcgcattttg gttagtatgg ggaggctaaa atggcatttt atttacccta
     1381 tctactcatt tttgttagta tttcaggaag tatttggctc atctataaaa tttttcaaac
     1441 aagatattca ttaaaaggct ctaaaattcg atttaagcga tttttctttc ttggctgtat
     1501 attttccttg ataatcgttt caagcggctt attcggcgtt ttagagggaa ataagcgcgt
     1561 ttctagaagt attttattag gaaatgtcac tcaaaagtat gaatcagcgc gtgataaaaa
     1621 gaaaaaagag caagtattag cacaaaaaat agaaaaattt accgcttgtt atgaggacat
     1681 gaatgatatt tttgtgaagc aagaaaagcg tttgacggat aaaaatatgg aaacactcac
     1741 tcgactttat cggaatttgc cagaagaaca gcaaaaagag taccaagaaa aatatgaaca
     1801 agtaaaaaaa gacgtgcaat atgtgaaaga tacgaaagtg gaagaagctt gtagtgattt
     1861 atttggagat acaattcctt tttcaactag cgaacaagaa cgaaaagaac gacaacaatc
     1921 agtcacatat gaacgctatg aaatcttgct tcaacaagca attaacatac aaaatctgac
     1981 taaaaaagaa acagctttaa attatttaaa aagcgtcaag gaatggttag atcagcaaca
     2041 gcaaaattga atttgattga aattcatgtt aaactaaaca tgcaaatgtt caaacattag
     2101 atacacgaaa gccccagaga ggtcggattc tctggggctt tttttaggtt gatgtcgcct
     2161 ttactgattt tacttcttgt ttcggttatt caaccaataa ctgaatacag agatacaaac
     2221 tcctacaaga agggggcaaa taaacagttc aaacatcaga tacacctcct tccagtcgca
     2281 agactgtcgg ataggcgaca agttattata ccctacaaaa ttaaatctgt gtggcaattt
     2341 taagtatctc ttatttccgg gttgttaggg cttgccctga ccgcctgtag ggcgctttaa
     2401 atgccagata aaggttttta tactaagact gtcttaaaac ggcttagaca agctcttaga
     2461 gcctcaaccc tcctttttga acaatggttt tagcaaagga gctggaggcg actgcgcagg
     2521 tataatgtga gagcgtagcg atcacacctt tttggcgacc gtagggagcg aggttttgaa
     2581 ttagcagttt agaaaagttc gcgaacctgt ttaaagaaaa aataattgtt tttttaggtg
     2641 ggaaattttg tctccctctt ttataatctt tttttaaacc tctttttaaa cctctttacc
     2701 gtcggctgtg tccttactcc cccaagggat acaaaatttt ataggcatga aattatctgt
     2761 ttataggcat gaaattatct gtttataggc atgaaattat ctgtttatag gcatgaaatt
     2821 atctgtttta ggtattgttt ttagacataa tatgtcctaa aatgatgtca ataaaatcat
     2881 ttagactgga gtaacaacta aatggcaaat gaaatcgtaa agtatgaacc agaattaaat
     2941 accattcctt taagaaaatt taccccaata gaaatgaact tgtttttttc tattgtctct
     3001 agaatgcgcg ataaaggtaa tcaaaaaata cgcttttatt tcagccaact taaagattta
     3061 agtgcgtata agcagactgc aggagaagtc tttataaaag atttggcaag aacatacgat
     3121 catttgatgg atttacgttt cggaagaaaa agcaatggtg gattaacaat agaacgattt
     3181 acaatgttta ctgattttga aattcatgga gaagtcgata acccttatgt agatataaaa
     3241 gtccacgaaa aagctttgcc tttacttaat aatctagaaa gctgggtacg tttctcttta
     3301 acagaatttc gagatttaaa tagcagttat gctaaaacaa tgtttagatt gctaaaacaa
     3361 taccgaactc aaggatgggc tgaattttct aaggaagatt ttttagagct attagatatt
     3421 cctaaaagct atagacaaac aaatataaat cagtttgtac tcaaaccaat aaaagaagaa
     3481 ctaactccac tatttaaagg attaactatt cgtaaaaaat atggtaaagg tcgaggtaaa
     3541 cctgttattg gttatcgttt tacttggaaa gctgaaatca accatgctga cgatttttca
     3601 aaagggaagc aagaagattt acgtataaaa ttatttaata ttgaacacaa tggagaatta
     3661 acacaaaaag aaaaatggcg tgctaaagat cgaatactaa atcttccgtt aggtactcat
     3721 gaagctgatt ttaaaaaaca acaacaaacc gaaaaagaag aagcggaaaa acaatcaatc
     3781 tcaaatgagc ttaaacaaga tttattagaa aacttacaaa acttattcga ttgaagcata
     3841 aggagtattt atgatagata aaacgatcaa agaactcgcg gaagaatttc atgtttcaaa
     3901 acaagcaatt aggaaaaggc tatcagctga ttttagagaa aactatgtgc aaactgttac
     3961 taggaacgga gtgaaaacct tagttgtcag ctacaaagga tatttgcttt taaaacaaca
     4021 ttttttaggt agtaacacca ctggtaacca agtagaaacc gttgctagta acgatgaaaa
     4081 ctcgattatt gtttttttac atgaacagct ccaagaaaaa gatactcaaa taaaacaaat
     4141 gcaaaagtta ctcgaccagc aacaacaact caccttgcag gcaaatcaac agatcgaaaa
     4201 actgcaagaa cagttacaat tagcttatac ggaagaacct gaagaagagg aattaacttc
     4261 tgtcaataaa gaaaacaaca atgcaataac aacagaaaaa aagtggtgga aattttggta
     4321 acaattacca actttccata aagaagaaat caatttctat agactctatc tttctaagaa
     4381 taaaaaatat gtttaggtga tcaaatgact ataagtaaag taatcaaaca aaaaagaata
     4441 gaaaaacaac taactcaaga agatttagct gaaatgcttt tagtatctaa aaaaaccatt
     4501 tctaattggg aaaatggtcg aacaatacca gatacagaaa atttaattca gtttgctaag
     4561 ttatttgatt tatcactaga caatattttg ttaggagaag aaaaaattat ggtaaaaaat
     4621 catacaaaaa cactaatact attatttcat tttgtaattt ttttaagttt agttttcacc
     4681 ttaattcagt ccggattctt ttattttagc cattcaattg ccacttggtt attaatattt
     4741 tttctaagtt tactattaat aattaatact ttaattatta tctttaataa acatagaaac
     4801 ttatttcttc acagcatatt tccaattgtt ttattaattt ttctatactt tatataaaaa
     4861 gatagaacta tttagtaaga taaaaaagta gctaattgta attttcgtct aaaactcaaa
     4921 actagtaaaa ataactttat tgtgttattt ttatttataa attatgaaaa aggacctttc
     4981 gaaatgaaaa ataatagaat ctacaaaaat cagttgaatt atgggctttt attgccagtt
     5041 tttctgctat gtatcattgg attactttct ttatatgttg ctttatatca cgactcttac
     5101 actaattcaa aaatcacaaa agaattaatt aaacaagtat tgtggtatag tattgggttc
     5161 acttcagcaa taactattta tttttttaat cctaaaattt tgtggaaatt aactcctata
     5221 ttttacggat tatgcatcat aatgttatta cttctcttga aattctataa tccacaatta
     5281 gcagtcttaa ctggagcaaa aaattggttt tatattgctg gtttttcgtt tcaacctact
     5341 gagttagtca aaataggata cacccttatg ctagctaaaa taaccgtcaa ttatcagcaa
     5401 aaatctatta ctagtaatac gaaagatgat tggatttatt tacttaaaac ttttattgct
     5461 actcttccaa taggaatatt attatttttt cagaacgatc taggaacaat gttagtcttc
     5521 attgttattt atatttttat tttgtttacg tctaacataa atataaaaat aattgcccca
     5581 ataatcgcca ttggtgttgt tttatcctta atatttttat atttggttat atcagataca
     5641 ggaagagatt tcttattaaa aataggattt cacgactatc agtttaaaag aatagactca
     5701 tggttgaatc catttttaga tccaaatgga tctagctatc agttagcaaa tagtttaatt
     5761 gctattggct ctggcggact tttaggtact ggttttaatg tatcaaacat tcacgttcct
     5821 gttagagaat ccgatatgat attttctgta attggagaaa attttggctt tataggaagt
     5881 tgtcttgtaa tattcctata cttttattta atttatcaaa tgtataaaac atgttttaaa
     5941 agcaataact tatttttcac ctatatcggt ataggcattg ttgcaatgat tttttttcat
     6001 gtttttgaaa atataggtgc aagtatcgga ttactacctc taactggtat tccactcccc
     6061 tatattagtc aaggtggttc tgccattctt agtaattttt tggggttggg atttatgttg
     6121 tgttgtagta gatatactaa cgaaaaaaaa ggaacacaaa aatgaattca aaataacact
     6181 ttatgaatag aatgtctatt tcaataaaaa ttatgatacc ttaaagttaa tcattaaaaa
     6241 aggagtgtca taatttgaaa aaaacaaaat tattggtagc tagtttgtgt ttgtttagca
     6301 gtttattagc tttcacccct tctgtttcat tttctcaaaa tggtggagtg gttgaagctg
     6361 ctgctcaaag aggatatatc tacaaaaaat atccaaaagg tgctaaagtg ccaaataaag
     6421 ttaaaatgtt agtaaatatt agaggtaaac aaactatgcg tacttgttac cttatgagtt
     6481 ggaatgctag tagtagaaca gccaaatatt attactacat ttagtaaaaa caaaacattc
     6541 tttctataca ataaaatagc gtatagagag gatgttttta atttgaaaag gagtggttat
     6601 ctttatgaaa aatagatcat ttgctatatt agtattatcc gtaattttat tagatatatt
     6661 gttaataaca ttagctttaa aaactttaaa tttagtaaca agttttgtca ttataccagt
     6721 aattatagga atgtctattt atttcatcaa aaaaaataaa caattttttt gaatgaaatt
     6781 acgggcgaaa ataatcactc aaaattatta ataaaaacga atcaactact ttttctgaag
     6841 aaacatacaa ccgtaaacaa caattgttgt ttacggttgt aaattatcaa actatatggt
     6901 gacttgttgc ttaaaatagg ttgaaatttc gtttattttt tttgagaaat tttacccacg
     6961 tactacccat gtacctatcc acgtattacc catgtacccc cccatgacaa atgggcggag
     7021 gggcttgcgg cgcttggtat taaaggcatt atccgcctcc caggtttctg accattttct
     7081 ctcataagtg gggtttttac cgcgttgaga gaaaatggtc agaaacctaa acgatcggca
     7141 aagaaaacat accaagcgcc acaagcaaaa aaagttagag taacttccag aaaagaaaaa
     7201 tttttagaac aacttttgaa aattt
//
LOCUS       DAZKNO010000136         7157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000136 DAZKNO010000000
VERSION     DAZKNO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.138"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..1315
                     /locus_tag="ACG8MY_002164"
                     /pseudo
     CDS             23..1315
                     /locus_tag="ACG8MY_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1834..2568
                     /locus_tag="ACG8MY_002165"
     CDS             1834..2568
                     /locus_tag="ACG8MY_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0431334.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            2584..3753
                     /locus_tag="ACG8MY_002166"
     CDS             2584..3753
                     /locus_tag="ACG8MY_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0431335.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            3769..4764
                     /gene="lacD"
                     /locus_tag="ACG8MY_002167"
     CDS             3769..4764
                     /gene="lacD"
                     /locus_tag="ACG8MY_002167"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFQ0431336.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            4843..5769
                     /locus_tag="ACG8MY_002168"
     CDS             4843..5769
                     /locus_tag="ACG8MY_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFQ0431337.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            5788..7038
                     /locus_tag="ACG8MY_002169"
     CDS             5788..7038
                     /locus_tag="ACG8MY_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0431338.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDANRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQKNKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2349 a         1195 c         1469 g         2144 t
ORIGIN      
        1 aagatcaaga ggtgattaca cgatggctag aaagaaaaga aatcctgatg ccgaaaagtt
       61 agctgaatcc attctgaatg cctatcaacc tgaatctgtc gatgacatgc aagatgcttt
      121 gaaagatgtg tttgggcccc tttttgaaaa aatgcttcaa ggagaattga ataatcattt
      181 aggttatgat gcccattcta aagagcctaa ggaacacgat aaccgtcgaa atggctatgg
      241 aactaaaacg cttaaaacca gttttggtga agtagctatt gatgttccta gagaccggga
      301 agcttccttt gaaccagagt taattcctaa gagaaaacga gatgtctccg acatcgaagg
      361 gaaggttctt tccatgtatg cacgaggaat gagtcaatga gatattgccg caaccgtcga
      421 agctatttat ggctttgata tttcccatga aatgatttca gatatcactg acgctgtcct
      481 tcctgaattg gaagaatggc aagcccgccc cctagcaaag tgttatgcct ttctattcgt
      541 tgattgtatg tatgttactt tacgagaaaa ttatgaagcc aaagaatatg ctgtacacac
      601 cattcttggc tatgatctca aaggaaataa ggagatttta ggattatggt taaatcaaac
      661 agaatctaaa aatcgctgga tgcaagtatt tgatgaactg aaagcacgtg gtgtcgaaga
      721 tgtctttttc atttcgatgg acggtgtttc tggcctcgaa gaaggcgcaa aagcgatctt
      781 tccatcggta attgttcagc gctgtattgt tcatcttgtt cgaaatgcat tgcgctatat
      841 tccaagtaag gactataagg aagtctgccg agatatgaaa aagttctacg gtgcttcttc
      901 tctaaacgct gcacatgctg cttttgacag ttttcaaaat cggtggtctc attattctgg
      961 tgctgtagat gtctggaaac ggaactttgc acatgttgaa caactatttg attatggtag
     1021 tgccattcga aaaattatgt acactaccaa tgcggttgaa agtgtccact ccagcttcag
     1081 aaaagtcaca aaaaaaggag cgttctctaa tgagaatgca ctcctaaaac tactttattt
     1141 acgcacgaaa gaattacacg cgaagtggtc aggtggtcga attcaaaact gggctatggt
     1201 gcttaatcag ttaatgatca acgagacctt ctcctctaga atgaaaagta tgcgatttac
     1261 cttccgtaac aacggtaggt ttattcaact atgtgtccga tcaaaattta tctgatttca
     1321 aaatagccga gagaattatg gtggttaaaa actcttaact atttaacttc cacagtttag
     1381 ttgacaaacc cacttaattc tgataacgaa aaacatattc gaatgattaa acagaagtaa
     1441 atacttattt aaataacttt caattttcat gttcataaat gatttgaaac caaagcaaat
     1501 gcgttgcact tttcctccgt cttccgaaaa tcgttcaatt cactggtaag tagtcatttg
     1561 atagtttgag gcaaattgtt gattgatgaa gggtaggagc taaaaaaaga taccagttga
     1621 agagagaaaa agaaaaaaat agagggtgtt cttgaatgaa aaaagttgga aaattatttt
     1681 tcatttcctt ttattcctat taaaatcagt gatgtcaatc atattaacaa gactaaaaat
     1741 gaaaataaaa aaaagattgt tcttagtatt gactcataaa aaataccact actatactta
     1801 cattgtggtt atagccagtt aggagagaca tcgatgcaaa atacctttca atcaaaatca
     1861 ctttattatc agctggtaga aatattgaaa caacgaatcg aaaacagtat ggtcgctcat
     1921 gacaagctgc catcagaaag agagttgacc agtttttatg gcgttagtcg cacgacggtg
     1981 cgactagcat tgcaagaact ggaaaaaatg ggctacattt atcgacagca tggaaaagga
     2041 acctttgttt cggaattaag gacaaatgcg actgatctag ctggcgcata tagttttacc
     2101 gaacaaatgc gggcaatggg caaacatcct gaaaccaaga tattggcctt caagaagatt
     2161 gaagcaacta aatatttagc gagtcatctg aatgttcaac taggtgagcc tttatttcaa
     2221 ttagaccgat tgcgcttagc tgatgatttg ccaatgatga tcgagaaatc ctatttgcca
     2281 gtgaagctat ttatgtccct ttctcaagaa attctatttc tgaaaccttt atataatttg
     2341 ttttcagaga atttcaatca aatcgtacgg ttggctgacg aggaatttta tgccagcatt
     2401 gtccagaaag aagatagtga gttgctaaat attccagaag gatcaccagt gctccattta
     2461 gttcgctcaa cctataatct cgagaacgaa gtgattgaat ttacattaag tgtagcgcgg
     2521 gctgataaat ttcattataa gatcaggcat ctgcgttcca ctaactgaat tggaggaaag
     2581 aaaatgttta tgttctcaaa agaagaacta cgaaagctag gagcagaaaa tactacccga
     2641 gaaattcgac aacaaccgga actgtggcat gagacgtttg accgttatca agaaacagtt
     2701 gcagatttag atcgtttgtt tacggatgcg aaacaacatg ctggagagcg accaatccga
     2761 gtcattttta caggggccgg aacctcgcaa tatgtgggag atacactaat tccgtttctt
     2821 actagtcaag gaaatacgaa ccagtttatt tttcaaagta ttggttcgac cgatatcgtt
     2881 gctacgccaa aagaatacct gattcctgat gagacaacga tcttggtctc atttgcccgt
     2941 agtggaaata gtccagaaag tctagccgca gttaagatgg ccaatcagct ggtcaacaag
     3001 atctatcata tcgtgatcac ctgtgcacca gacggattat tggctacaca aatgcctaca
     3061 aaaaaagaaa atctagtttt actaatgcca aacaaagcca atgacgctgg ttttgctatg
     3121 acaagcagtt tcagctgtat gacgctgact gcgctactta tttttgatca agcaagcaat
     3181 cagcaaaaag aaagatatgt tcatcaatta agccgactag gagaagaagt catccaacga
     3241 gaatcagaga tccaagggta tctggcatca gactttcatc gcatcattta cttaggttcg
     3301 ggaagtttag gcggattgac tagagaagcg caattaaaag ttctcgagtt aactgctgga
     3361 aaaatcgcaa ccgtatttga tacatcttta ggattcagac atggtcccaa atcttttgtc
     3421 aatgagcaaa cattggtttt cctgtttgtc agcaatgact catatacgca gaaatacgat
     3481 gtagacatgc tggaagaaat caaaaatgac ggtattgccc aacgcgttgt tggaattggt
     3541 caaaagggtt cattgtttag tggtgattcc tttgaattag ttgcagattc gttactgccg
     3601 gatggctatc ttgctttagc agatatcatg gtagcccaaa cgatcgcatt actttgttcg
     3661 gtgaaggttg gtaatacacc agatacacca tcgccaagtg gaacagtcaa tcgtgtggtc
     3721 aaaggcgtaa cgatttatcc atatgaaaaa taggaaagag tgtgaagcat gttagaatgg
     3781 tcggaaaata aaagacaagc aatggaacgt ctatcgacgc cagaaggcat tattaccgct
     3841 ttggcgatcg atcagcgagg cgcgctgaag aaaatgatgt cggctttagg gatggaagcg
     3901 acaaaggaaa aaattgagca gtttaaaaca ctaatttccc aagaattgac tccttatgcc
     3961 tcagcaattt tattggatcc tgaatacgga ctgcctgctg cacagaagcg tgcagcagcg
     4021 agcggtctgc tgttggcata tgaaaaaact ggttatgatc aaaccgtacc cggccgcttg
     4081 ccggatctgc tgtcggactg ttcagttctg cgattgaaag agttaggtgc ggatgcggtg
     4141 aaatttttac tgtattacga ttatgaagaa gcagaggaaa tcaatcggca aaagcaagtt
     4201 tttgttgaac gaattggaag cgaatgcatg gctgaaggac tgccatttta tttagagtta
     4261 ttgtcatatg atgctaaaaa tgtagatacg aaatcgcttt cctatgccaa agcaaaaccg
     4321 cgcaaagtga tcgaaatgat gaaagaattc tcgaaaaccc agtataaagt agatgtctta
     4381 aaggttgaag taccagtaaa catgtcattt gttgaaggaa atgcaacagg ggagatcgcc
     4441 tatacacgtc aagaagctgc cgcttatttt aaagaacaaa gcgatgcaac agatttgccg
     4501 tatatcttct taagtgctgg ggtatcagcg gcattattca tggagacatt gcgttttgcc
     4561 aaacatgctg gggcacgatt caacggtgta ctctgtggca gagcaacttg gaaagatgca
     4621 gtcgctcctt ttgttgaaaa aggagaagcg gcaactttag aatggctgac agaacaaggg
     4681 acacaaaaca ttcgtcaatt gaatgaagtg ataagagaaa ctgccatccc atggtatgag
     4741 aaagtaagtg aaagcacttg ttaatgtttc taatatatta gtagtgataa gagatatgtg
     4801 tgattataat ttatcattat tgaaactggg aggatatttt atatgaataa aagattgtta
     4861 gattattcag cttcagattt tttgaaagct aagccgatgg aattaaagca ggcaattttg
     4921 gcttctgaag gtagaacaat tatggcagaa aatgtgccaa gtagctgtcc ttacatggga
     4981 aaatcagtga caaatgcaga attggagaga gctgctggag ctgatttaat tttgtttaat
     5041 gcactagatc tttttaatcc tcaaatttca ggtgtacctt taactctaaa agagaatcca
     5101 ataatctgga ttaaaaaagc tttaggaaga gctattggtg taaatttaga acctgtagac
     5161 aaagaagttt tgatgagtga aaatcgaact gaaatttcaa gtggtagaat cgtttctcct
     5221 gagactttaa aaaaggcaaa tgaattaggc tttaatttta tttgtttaac tggaaatcca
     5281 ggaacaggag tatctaatag aactattgag aaagcaataa cattatccaa atcaattttt
     5341 aatggattaa ttatcgctgg aaaaatgcat ggtgctggtg ttgatgaacc aattatgaac
     5401 ttagatatag caaaaagatt tattaaaaca ggtgtagata ttttattagt tcctgctcca
     5461 tacactgttc cgtatttcat ggaaagtgat ttgcgaggaa tcgttgatta tataaaagat
     5521 tttaatagaa ataaatctat tgaggaaaaa gttctagtac taacggctaa tggaacaagc
     5581 caagactctt ctgaccaaga aactattaaa aaaattgcat tagcatcaaa agcatgtgga
     5641 gctgatattc aacacatagg ggattcttta aatggtattt gtcttccaga aaatattttt
     5701 gctttgggtc aagcaattcg gggatatcgt catcaaatta ttatgttagg aaaatctaat
     5761 tatcgttaat taaggaggaa atgaaagatg atcgacaaag ttcaaaaaat agctgcaaaa
     5821 attagtaatc aaagacatct actagcttta agagacagca tggctgcagt tatgccatta
     5881 atcattattg gttccttttt tatgttatta gcaaatttcc cgatagaagg atttacaaaa
     5941 tggttatctg aaattggagt acaatccttt ttgaacaaag catcagattc aacatttggt
     6001 attattggat tagctgttac ctactctttg ggacacaatc tagcaaagac ttatggtgta
     6061 gactcattat cagcaggatt attatctttg agtggatatg ttttacttac acctttattg
     6121 gatgcagatg gttctatagg atttccaaca aaatactttg gtacctcggg tctttttgtt
     6181 ggtattattg tagcacttgt atctacagaa attttccggt ggtttgtact taaaaatatt
     6241 acaattaaaa tgccggcaag cgttccacca aatgtaagcc gagctttcgt tgctattatt
     6301 cctggctttt ttgtcgttct actatggttt attgttgttg taatttgtta taaacttgga
     6361 attgaaaatg ttcatgctct tattgcagat acactcgcaa aaccactatc tttactgacg
     6421 aaaactttac caggaattat attggttata cttattcaat gtttcttttg gatgtttggt
     6481 attcatggtg cacaggtaac ggggccaata attgaaccgt tattattgca aaactctgat
     6541 gcaaatagaa ttgcttatca agctggaaaa gaacttccta acattataac ttatgaattc
     6601 ttatataact ttgttttttc tggtggtgca ggttgtttat ttgcacttgc cattttaaca
     6661 tttttctttt caaagagtca gaaaaataaa actttaggta agttatcaat cgctccagta
     6721 agttttcaag tagcagagcc ccttctattt gggtttccaa ctatattaaa tataaaaatg
     6781 ttaattccat ttgtaactgc accagtagta acaacattaa ttacttattt ttcaatgagt
     6841 atgggattag ttgcaaaacc tgtaggagca acaatcccat ggacaactcc accgataatt
     6901 gccggatttt tagcttctgg tggacgaata agtggtgcag tgattcaagt aataacaatt
     6961 gcaataaatg ttttgatata ctatccattc tttaaattag atgataatgc caagctaaaa
     7021 agcgaaaaaa atgattagaa ctacctatat gtttctaact gtagttaagt atgaaaagac
     7081 tatttttaaa tttagcattt ttgcgctatc ggcgtagatg aagtatcacg ataacgaaat
     7141 tagtttcttt catttag
//
LOCUS       DAZKNO010000137         6976 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000137 DAZKNO010000000
VERSION     DAZKNO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.139"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            24..1178
                     /locus_tag="ACG8MY_002170"
     CDS             24..1178
                     /locus_tag="ACG8MY_002170"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0431339.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1200..1952)
                     /locus_tag="ACG8MY_002171"
     CDS             complement(1200..1952)
                     /locus_tag="ACG8MY_002171"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0431340.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2316..4982)
                     /locus_tag="ACG8MY_002172"
     CDS             complement(2316..4982)
                     /locus_tag="ACG8MY_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0431341.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5323..6774
                     /gene="cls"
                     /locus_tag="ACG8MY_002173"
     CDS             5323..6774
                     /gene="cls"
                     /locus_tag="ACG8MY_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0431342.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2053 a         1447 c         1273 g         2203 t
ORIGIN      
        1 atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc ttttttattc
       61 ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat cctgcaaaat
      121 accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa gacatcatta
      181 cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag ctaatcgaca
      241 tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg gtaattatcc
      301 accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag gcttgctacg
      361 gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca gaacgaaaag
      421 tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt gaagtgacgc
      481 aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta tcgattgaag
      541 acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat tatagcgaat
      601 ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc caaaaagttt
      661 ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg attgctttcc
      721 atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac caaacagacg
      781 aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac agccgacgaa
      841 tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg gaaaattcga
      901 aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt gcagtaagtg
      961 aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca caatctcatc
     1021 aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg attttcagtg
     1081 agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct ttttcgatta
     1141 caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa aagaaccctt
     1201 cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct ataggtcagc
     1261 ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta cctggtcaca
     1321 acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt aattgattcc
     1381 tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat taagtatgtt
     1441 tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat tcctagttgc
     1501 ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat cacgcaccat
     1561 ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc tggatttgct
     1621 gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa cgactgtttg
     1681 ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt ttctagcatt
     1741 cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga aagaaatctg
     1801 ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc cgttttcaga
     1861 taatttttta catcttcagc aatagttact tgcgattctt gctggatttc ataatattct
     1921 tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt ttgttatatg
     1981 ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct tttgatgaat
     2041 aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga ccaaagtcgt
     2101 gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag tattagctca
     2161 aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc ttcggtcaca
     2221 agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt gtgactcaga
     2281 attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg tactcgtaca
     2341 atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc catacatgta
     2401 aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag tcctgggatc
     2461 attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact tctgttagag
     2521 aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt ttgcaatgtc
     2581 cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga atgctgcatg
     2641 ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata cgttacagaa
     2701 atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg gttcatcgta
     2761 gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt gactaaattg
     2821 atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa ggcaaagatg
     2881 atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt cttgatatta
     2941 tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt atcatccgtc
     3001 aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc aattccgatg
     3061 tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc tgagatatga
     3121 cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac acgtgcataa
     3181 acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc tagttccgtc
     3241 ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat agcaacagct
     3301 gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct tgcttctgtt
     3361 accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc aacaaggatc
     3421 aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta agcaaaagct
     3481 agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt tttcatcaat
     3541 tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg ttggaagacg
     3601 atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg tccagtagac
     3661 attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt gtttcggatt
     3721 tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc cgtagggtcg
     3781 cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact gatttcgatc
     3841 aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc agtatcgcca
     3901 ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc agtcttatct
     3961 gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg aataatggca
     4021 tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat agactgtaat
     4081 gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct gacgatatct
     4141 gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat acctaaaatc
     4201 aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc cagacctcgt
     4261 tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg tccgatctcc
     4321 gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac gatcgttcca
     4381 ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc cgttacttct
     4441 ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa tgatcccgca
     4501 tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac gatatctcct
     4561 accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac tcgagcatcg
     4621 ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt ttggacaaca
     4681 ctgattatag aattcaacat caatactgca aaaataacta agaattcgat gtagtcaccc
     4741 atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc tttgaaagtt
     4801 tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt caagccatct
     4861 ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct ctgcgtttgc
     4921 tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc cttgttttcc
     4981 aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa aagagaccta
     5041 agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa aaccatttgg
     5101 ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta aggagatatt
     5161 caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta gcccgattta
     5221 cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc tttcttctat
     5281 catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc tagtattata
     5341 accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat caaaccccga
     5401 gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg ggtgggcttt
     5461 gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta tctccgacaa
     5521 agtgacttga aagaattaga aaactttcag tcttttagag gagataccat tgaacattac
     5581 gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc ttcattaaat
     5641 cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca agagaaattg
     5701 gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga atattacgca
     5761 tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa agccgcagaa
     5821 ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa agcaaaagat
     5881 ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc acaaagggca
     5941 ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat cgatggaaag
     6001 attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac aaaaaaattc
     6061 ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt gctccagctt
     6121 cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg ctatcatttg
     6181 aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac atccatccag
     6241 cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt tatcaaattg
     6301 attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc tgatgaaagt
     6361 gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat catgattccc
     6421 aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg gcagctgatc
     6481 aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa aacattgata
     6541 atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag ctaccgattg
     6601 aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact tgctactcag
     6661 ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa ggaaatgtct
     6721 aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct ataatttttt
     6781 tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat cttgtcacag
     6841 tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
     6901 caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc tggacggcag
     6961 gctcacgacc tctttc
//
LOCUS       DAZKNO010000138         6948 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000138 DAZKNO010000000
VERSION     DAZKNO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.140"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            210..2252
                     /locus_tag="ACG8MY_002174"
     CDS             210..2252
                     /locus_tag="ACG8MY_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0431343.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2263..2883
                     /locus_tag="ACG8MY_002175"
     CDS             2263..2883
                     /locus_tag="ACG8MY_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0431344.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2895..3353
                     /locus_tag="ACG8MY_002176"
     CDS             2895..3353
                     /locus_tag="ACG8MY_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0431345.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3372..3650
                     /locus_tag="ACG8MY_002177"
     CDS             3372..3650
                     /locus_tag="ACG8MY_002177"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0431346.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3675..5042
                     /locus_tag="ACG8MY_002178"
     CDS             3675..5042
                     /locus_tag="ACG8MY_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0431347.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5057..6202
                     /gene="dgoD"
                     /locus_tag="ACG8MY_002179"
     CDS             5057..6202
                     /gene="dgoD"
                     /locus_tag="ACG8MY_002179"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFQ0431348.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6229..6720
                     /locus_tag="ACG8MY_002180"
     CDS             6229..6720
                     /locus_tag="ACG8MY_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431349.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
BASE COUNT         2192 a         1134 c         1507 g         2115 t
ORIGIN      
        1 agcatccaat attctaccga atatttaata ttcacattag ccctaaaata attgtcatac
       61 catgttacat ttgatataac agcgttcatt ttaaaaatag ctgttagtgt gacaaaacat
      121 taaaccaagt gtgacaaaaa acgtgatgaa agctttccgg aaacgtatgt gaacaagtat
      181 ataatgattt tgtcaggagg atagtcaaaa tgaataaacg aattcgtgaa atactcaaac
      241 tgattattaa aaaccctgaa atgaaacttt ccactttaac aagtgaactt gatttgacac
      301 gtcgtcaaat caattacgcg atcaatcaat ttaatgaaga attgaaaaca aagaacattc
      361 cgttaattca gagaagtcat tctggaaata ttacggtacc aatcgaggtt atccaaatga
      421 tatcacagcc tgattacgac ataggtgatg atcaggaact tgtcgttctc actgatagtg
      481 aaagacaagc attgatagtg atgactctga tcgctaatat cgattacatc agtctggatc
      541 atttggtaga tgcggttgat gttagtaaga cgactgttat ggaagatatc aaacgaacgg
      601 aaccgttcat cagaaattat agtctgacaa ttcgatataa tcggatgaat ggttaccaat
      661 tggttggttc ggaacaccgt atcttacagc tgttatcgga tctagtaaaa cggtttccga
      721 tttttcgccg agaaatgatt cgcagaaaat tggcacaaga tgtttctgaa gaagaaatcg
      781 tccatttgat ccataacatg gaacaaatgt tgcatcttag ttattctgat gagtcggtag
      841 actatttgca gacatcagcg agatttttag taagccgtgg gatcaagatg tcagataagc
      901 acgcgttttt tcccgatgat gtgacggata ctcctgaatt ccgaatgctg atgatcttgg
      961 tacatgaaac aggttggagt ctatccaaaa gttatatgga gtggttcacg ttattatttt
     1021 taacttccaa tatttttgag agcaaaacta ctcaaaatta tgattcggat caagaattgc
     1081 gaaaattgat cagccaaatg gttgaaaatt tccaaaatca aacgttgatt ttgatcgatg
     1141 accgagaaga ctttgaacgt aggatactga gtcatctgcg tccagcctgt tttcgaatac
     1201 gctataactt gagtttagga gtttattcaa tagatagtct gatcaaggat agtaatcatg
     1261 cgattttgat tgatttgatg aaagaattga ttattcctat tgagaattgg ctaaataagg
     1321 cctttccaaa tgatgaatta gatcttttat cctattactt tggctatcaa ttaagtaatc
     1381 ataataaatt gaataagcaa cgacaaaggg ctgtagttgt ttgtactaat ggtgtcatgg
     1441 tctcaaagtt agtacgagcc aatatggaaa aactttttcc agagatccat tttttagctt
     1501 ccctatctgt gcgtgatttc tacaaatttg aagctgatta tgatctggtc tttactacga
     1561 cgccactaaa aagttctatg ctgcagttta tgatcgagcc aatcatgaca tatcagcaac
     1621 aaatcagttt acgatatcga gttttgagtg atttaggaat tactaaggtt gatcgagcaa
     1681 tggatgaatt gttaacaatc attaggaaat atgcaaatat caccaatcca gtttcgttgc
     1741 gagaagaact gcagtacttt ttgatcaagg aagatggaag cacgccgctg gacaacttca
     1801 atgtattgcc ttcattgact tattacttgc ggccgagtta tgtacaaatc gttgaagaag
     1861 agatgtcttg ggaacgagca gtggaggtgg cttgccgacc gttattggaa catcaaatca
     1921 ttgactcgcg ttttatcgct gattgcttgg atcaaatcag acaaccggga tatgcaggtt
     1981 acttgggaat gaagacttgc attccccata caacggtcga taaaggtgta atcagtgacg
     2041 gtgtcagcat tttagtttgt aaaaagccga tacgatttcc taatggtgaa gatgtcagct
     2101 tgattttgcc gctttcattt tttgatttaa cgaagcatct gcgagccatc aatcaaatcg
     2161 ctgatatcgc caaagatgat tcattgattg agcagttatt gattgctgat gacgaaaaga
     2221 cagtttatca actattaaga caaagatctt aaggaggaaa acatgagact agcagattat
     2281 cctaaattga cagtgatcat gcgtggttat acttttgatc aggcagatgc tattttacaa
     2341 gcaatgcagg aatttgacca tcaatttgcc gtagagatga caatgaacac aaaaggagct
     2401 cttgaaaata tacatgaact aaatcaacgt tatggtgaca cgacttatat cggcgctggg
     2461 acggtaagga cattagaaca agcccaagcc tgctatgagg ctggtgcaaa atttttatta
     2521 ggaccacata tgttcacaaa agaaatgttg tcttacgcca ataagaaagg attattggcg
     2581 gtacctgccg caatgactcc ttcagaagtc gaccgtatgt ttaaagaagg agctgatatt
     2641 gtaaaagtat ttcctgcagc ggttgttact ccacgttttt tcaaggatat tcaagcacct
     2701 ttaggtaagt taccactaat gggggttggt ggaatcagta aggagaatgc gaaagagttt
     2761 tttgaacaag gtgctagcta tttaggtctt ggttccggta tgttcaacaa gcaagacatc
     2821 gaagagctaa atgttaaaaa cttagcacgt tcgatgaaag aattaattga ggctattaaa
     2881 taggagtgag gactttggaa ctttatttga atgaggaact cgtttttcga aatgttcaag
     2941 caaaaacaga tagtgaagtc ttagcgtttt tagcaagcga gatgtacaaa aaaaactacg
     3001 tgaaggagga atatattcaa gcaatccaag aacgggaaaa agaataccct actggattac
     3061 catcaacacc accaggaatc gcaattcccc atgcaaacta tgaaatggtc aataaaacaa
     3121 ctttagcaat tgcgactctt aaagatccgg tcctatttca taatatggaa gataacaatg
     3181 agcagttacc aatccaaatt gtgatcatga tggcgattgg ggaaccacat gggcaagttg
     3241 aaatgttaca aaaaattgtt ggaatcatac aagatgagcc actgcggcaa gaaatgatca
     3301 gagcaggcaa tgatactgaa ttattagaac tattaaagaa agcggttttt taatataagg
     3361 aggaaatcat aatgaaacga attttagtag catgcggaaa tggtatcgcc acttcaacag
     3421 tagtagcaac aaaagttaga gagtatttgc aacaacatgg cattgatgtt tcaacgactc
     3481 aaacaaaatt gatggaagtt ccaggaaaag ttcaagacta tgatctattg gtgacgactg
     3541 gtcaatttga tggtcaaaca ggtggtgtac cagtaatcaa agggatgccg attttaacag
     3601 gtatcggtgc cgatcagaca atggaagaga ttttgaactt actgaaataa tagctgatca
     3661 aaggagctga aaaaatgaaa atcattatga acgtctttaa cttctttatt gatgctggac
     3721 caacagttat gttgccagtc atcatcacga ttattggttt gatttttggg ttaaagattt
     3781 ctcgtgcttt taaatcaggt ttgacattag gtatcggttt tgccgggatc aagttgatat
     3841 tggactttat gacgaccaat gttggaccag ctgccaaggc gatggttgat cgaactggtg
     3901 ttaaactaga tgctttggat gttggctggg gttcgattgc ggctgttact tgggcatcgc
     3961 caatcattcc aatcttgatt tttgccattc tattggtcaa tatcgttttg ctgattttga
     4021 aacgtacaca tacgctggat gttgatatct ggaactatca ccatatggca atcgttggtg
     4081 ttatagtgta ttttgtgact aagaatgtct tcttaggtgt aggtgcatca gtagtgatgg
     4141 caattgcgac ttttaagatt tcagactggt cgcaaccgat ggtagaaagt ttcttcggaa
     4201 tccctggtgt ttctttacca actgtttctg cgctttcatc actggttatt gcttggccgt
     4261 taaattggct gctggatcgt atcccgttat tcagaaaatc aaaattcacc atcaaagatg
     4321 cacaaaaata tttgggattt tttggtgatt ctatgatcat ggggttagtt attgggatgg
     4381 tgattggtgt tttagccggc tatgatatca aagcggtttt acaattaggt gtcagcatgt
     4441 cagctgtttt ggttttgatt cctaagatga cttcattatt tatggaagga ttgatgccaa
     4501 tttcagatgc tgcacaaaaa tggtcacaaa aaaaattcaa aggtacacgt ttatttatcg
     4561 gattggatgc tgcagttgtt gtaggtaatc cagatgttat cacaacagca ttgatcatta
     4621 ttccactaac gattgcatta gctttagtat tgccaggaaa ccgtgtgtta ccattcgcag
     4681 atttagcggt tgttcctttc cgcgtggcaa tggtcgttgc cttgactcgt ggtaatttat
     4741 taaaaaatat cattattggc ttagttgtga cagcttcatt gctatggtgt gggtcagcta
     4801 ctgcgccaat gctgacagca attgccaaat cggttggtat taacttagga acttcttcaa
     4861 tgttaatctc atcatttgct gcgacttcca tgatccaaag ttatttggta tttatcgcat
     4921 ttgcttacaa accaatcatc ggtattccag tgttgttatt agcctttgcg ggcgcatggt
     4981 attacttcga tggtatcaaa aaagttaatc atgaaatgga tgaaccattg aaggcacaat
     5041 agtaggaggg tttaaattga aaatttcaga tgtcacagtg tataaagtaa aaccaagatg
     5101 gatttttgtt aagatctcga ctgacgaagg aatcgaaggc tggggagaaa tgatctcagg
     5161 aactaaaaca gaaacggttg ttgctggagc ttatgagata ggaaaccggc tgattggacg
     5221 taatccgttt gaaatcgagc gcctcttcca agaaatgcat cgatctttct tccgaggcgg
     5281 tcctattaat ggaaccatcg tttcgggttt agaaatggct ctgtgggata tcaaaggaaa
     5341 agcgtttaat gttccggttt atgaattatt gggcggtgca gcacgtgata aaatcaaggt
     5401 ttattcttgg attggtggag atcgaccaag tgaagttgca gaacaagcac aagatcggtt
     5461 tgatcggggc tttacggccg tcaagatgaa cgcgacagaa gagctgcatt atatcgattc
     5521 ctaccaaaaa gttgatgaag tggtggaacg agttgcgtct attcgcgaac gttttggtga
     5581 tgcaatggat attggtgttg attttcatgg acgggtccat aaaccgatgg caaaagtgct
     5641 agcaaaagct ttggagccat tccatccaat gtttttggaa gaagtcgttt tacctgaaaa
     5701 tgaagaacac tttaaagaag tagctgattc tgtggctgta ccattagcta ctggtgaacg
     5761 cttgtacact cgttggcaat tcaagaacct ctttaaacaa ggtactgttg atattatcca
     5821 accagatgtg gctctttgtg ggggaatctt ggaaacccgt aaaatcgcag cgatggcgga
     5881 agcttacgac atggctgttg cgcctcatgc gccatatggt cctgttgcac tagcagcaac
     5941 tctgcaagta gactcatgta cgccaaacgt ctttatccaa gaacaaagct tagggatcca
     6001 ctataacaaa ggttttgatc tgttagattt tgtgaagaac aaagaagttt tccaatataa
     6061 agacggctac gtagatctac cttcaaaacc aggtctgggc ttggaaatgg atgaagacaa
     6121 aatcaaagaa attgcacaag aaggtttgat ttggaccaat ccgcaatgga aaaattatga
     6181 cggtacgatc gctgagtggt gataaatgaa aataaaggat gatgaaagat ggcagaagca
     6241 cttatgatca cagttttttt aggactgctg attattttgc aaataatcga tcaaaagaaa
     6301 attatcgtgt ttgctcagag tagtcctgta aggaagagtt tgagtatttt actgacagtc
     6361 gccatcctgt tgattttctg gccggaacat ctgactgatc agattaaact ggttgctttt
     6421 gcttctgtca ttttgatcaa tggttttttg aaggagggtc tagcaaaaga ttctgttgtt
     6481 agatttggag tattgaacgg taactttaat cgatatcctc aagtccaagt agaaaaacta
     6541 ggtgacgata aatcatttat ttcattttac aaaagcaaga ataattcttt ttcaatgatt
     6601 tttgataaag atcaaactga aatgatggat gtctttcaag aacttgatat ccattccaaa
     6661 attactttag atcaaagttc ggaagaaaaa agagcagttg taaaaaaaga taatgaatga
     6721 ttctactacc gaaattatga tttctttatc acgaataatg aaacttgttt taatgaatca
     6781 tattgattga agtatacaat gtatattaag ttttggaaag aaatatattg gtacaagata
     6841 tagcaggaat cgattactcg atccctgctt tttttaactt cctttgcagc aaaaaaagct
     6901 cgtaatctaa gagatgatgg atagttttta gaatatatat attttctt
//
LOCUS       DAZKNO010000139         6920 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000139 DAZKNO010000000
VERSION     DAZKNO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.141"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..568)
                     /gene="vanZ-A"
                     /locus_tag="ACG8MY_002181"
     CDS             complement(83..568)
                     /gene="vanZ-A"
                     /locus_tag="ACG8MY_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0431350.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(721..1632)
                     /locus_tag="ACG8MY_002182"
     CDS             complement(721..1632)
                     /locus_tag="ACG8MY_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0431351.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(2060..2668)
                     /locus_tag="ACG8MY_002183"
     CDS             complement(2060..2668)
                     /locus_tag="ACG8MY_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0431352.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(2674..3705)
                     /gene="vanA"
                     /locus_tag="ACG8MY_002184"
     CDS             complement(2674..3705)
                     /gene="vanA"
                     /locus_tag="ACG8MY_002184"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0431353.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAFITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(3698..4666)
                     /locus_tag="ACG8MY_002185"
     CDS             complement(3698..4666)
                     /locus_tag="ACG8MY_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0431354.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            complement(4881..6035)
                     /locus_tag="ACG8MY_002186"
     CDS             complement(4881..6035)
                     /locus_tag="ACG8MY_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0431355.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(6013..6708)
                     /gene="vanR-A"
                     /locus_tag="ACG8MY_002187"
     CDS             complement(6013..6708)
                     /gene="vanR-A"
                     /locus_tag="ACG8MY_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0431356.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
BASE COUNT         1938 a         1536 c         1222 g         2224 t
ORIGIN      
        1 ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg tatgaaagat
       61 cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta cggtaaacga
      121 gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat tttttttgat
      181 tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca acagtatttg
      241 taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt atttcaaaag
      301 taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct ttaaaattga
      361 cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc tctctgaaat
      421 tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta aatactgata
      481 aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa tataaagcta
      541 gcaatcctct agataatatt tttcccaata taatttcctc taattctttt gtttaagttg
      601 tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag ctaacgttac
      661 cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat tccgccatcc
      721 ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta caccgtctat
      781 attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg tatttttagt
      841 aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa tggttttttc
      901 ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca taatcgcact
      961 atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg ttaactctgt
     1021 tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt ctatccactt
     1081 tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg ataaacctga
     1141 attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt ggtaaagcac
     1201 actttgctca tcaaagtctc gatagccact attaataata aaatgactaa cgccaccctt
     1261 tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca tataaatatt
     1321 actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat tcacgatatc
     1381 tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca gatttccttg
     1441 ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc cactattttc
     1501 taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct gagaaaacag
     1561 tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca ataaaaaaaa
     1621 caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga aatatttcta
     1681 gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc cccgtaaatt
     1741 acagtcaatt atttcgccct actggattta ctattatcac caatgtagat gaattttcat
     1801 aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg ggcggtgtga
     1861 aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca gcgcacgcag
     1921 ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat atataaattc
     1981 cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta tatagtttgt
     2041 ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg gggtatggtt
     2101 cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca ctgttttcca
     2161 tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca tttgccgcat
     2221 gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc tcacccgtgt
     2281 ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat tttgaagcca
     2341 cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt tcctttgtca
     2401 ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacgc ttaggacggt
     2461 aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt gccttcaaaa
     2521 gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg tcaaccggtt
     2581 ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg tgtactattt
     2641 catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta atacgatcaa
     2701 gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg gataacgact
     2761 gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc cgttatcttg
     2821 taaaaacata tccacacggg ctagacctct acagccgagc gctttatata ttttttttgc
     2881 cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg ttataaatgc
     2941 gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc cgtactgcag
     3001 cctgatttgg tccacctcgc caacaactaa cgcggcactg tttcccaata ccgcacaacc
     3061 gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt gtcttgccga
     3121 ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga aggatgagcc
     3181 tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg gcctatcatc
     3241 tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa cgatgtatgt
     3301 caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa aagggatacc
     3361 ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat gcaaagctga
     3421 aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa gtaatccgtg
     3481 cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc attccgcgca
     3541 aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata acggctcgta
     3601 tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata cgtcatgctc
     3661 ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc tgtctccttt
     3721 caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct tgctcggtat
     3781 aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta agtaaaaatt
     3841 gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct ccttccaata
     3901 catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac tcataggtat
     3961 ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt ctctgtattt
     4021 gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc gtaacgatat
     4081 cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt cggctgcgac
     4141 tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct ttgcctatct
     4201 ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg tcgctgtcca
     4261 acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt actgccataa
     4321 gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca ttgtcgacag
     4381 tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg cttcgggtag
     4441 aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa atctctgatt
     4501 tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat tccgacacgt
     4561 tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg aatgcatctg
     4621 cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt aagaccaacc
     4681 ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt caaatctttc
     4741 tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa gaacgagccg
     4801 tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt gagattttcc
     4861 taaaaaatta tatacatctc ttaggacctc cttttatcaa ccaagtctgg catcgctgga
     4921 agctctaccc taaacgtcgt atagttatca ttgctttccg cgtaaatctg ccctccatgc
     4981 tgaacaataa tttcttttgc aatcgccaat ccaagtcccg cgccacccgt atcggaagaa
     5041 cgagcattgt ccagcctata gaacttttca aatatggcag ctagcttatc ttttgggatg
     5101 cttccagtgt tcttgaattc gattgacacc acatccccgg agaggcccgc ggtaatgtca
     5161 atgatgctgt tatcctcact gtatgcagcg gcgtttttca aaatgttgtt aaagactctc
     5221 gcgagtttat cagggtcgcc ggacacggtc agatcctcgg gggcgtgaat aaccgcctgt
     5281 tttccatgtg cggaaagctg aggataaaat tcatcggtca tctgcaccag catatagtat
     5341 aggtctatgt gcgtttttgt tagcgttatc gtttgtaggt tataccgtgt aatctcaaaa
     5401 aactcgtcga ttagctgttc gagtcgatac gctttgtcca acgtgatatg cacatacttt
     5461 gccttttgat ctaccggcat gtctggagcc tcgtcaagca ggctcaaata accgataatg
     5521 gatgtaaggg gcgttttaat atcgtgcgcc aagtacataa caacgtcatt ttttctttgt
     5581 tcggccagct ttgcatcctg ctctcgcttt tccagagtcc gttttaatgt gttgagcttt
     5641 tgttccataa catccatttc cgcagaaagc tcaatttgtt tatcttcgtt ctgaataagt
     5701 acatcaatgc cggtatttat ctcgtcaaag tattttgcga attttgaaag catgacgcga
     5761 catagaataa gaatactaat gacaatcgcc acataaataa agatatctat attgttccgt
     5821 atggaatatt gatataattt catcgcgtcc aggtgattta agtcatattt gttttccaaa
     5881 atacttaaga tccaatcccc aagtttccct cggatcattg aacgaatata caacacgaat
     5941 acaattgcta ccacaacaat tgcaacgata tacatgtaaa gttttcgttc tagtttggaa
     6001 tagtcgtttt ttttattttt caattttata accaaccccc cataccgttt ttatatattt
     6061 cggattatca atggtgtcgt tcattttttc gcgcaaatgc cggatatgca cggtgatggt
     6121 gttgttgctc ttgctgaaat attcgtcgcc ccatatctca tgaaatagca gctcggagct
     6181 aaccacattc cccttgtttt cacagaggat tcgcagtatt gaaaactcgg tgggagtaag
     6241 ggataactgc ttctcgttca gataacactc atgggtgtta acattaatga caaggccgga
     6301 gtggacgata acattttcgt tctgctcctt tactccactg aattttttgt atcggcgcaa
     6361 ctgggccttt acccgagcaa ttaactccag tgggcgaaag ggcttcgtta tataatcatc
     6421 cgcgccgatt gttaaccctg taattttatc tacctctgta tctttcccgg tcagcatgat
     6481 aatcggatag gtgtgcttgt cccttatttt ttgacagata gtaaggccgc ttgtgccggg
     6541 aagcatgatg tccaatatgg caaggtcaat ctcagacttg tctatacatt ccaatgcttc
     6601 tttggcggta tagtatttga aaaccgtata attctcgttt tttaagtata attcaaccaa
     6661 atcggcaatt tcatgttcat catccacaat aagtatttta tcgctcatag ttatcaccct
     6721 ttcacataag tttgccctta ataatttatc agattatagg gccgaattta taagcattta
     6781 cataataaat gactaagaat ttcttaagat gatgattctt tttttatcaa agggcttttc
     6841 ggcatcgcta ggaggctgtt aatattttat agtttccttt tcttctttaa aaatattgcc
     6901 cattagcgga atacagaatg
//
LOCUS       DAZKNO010000140         6685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000140 DAZKNO010000000
VERSION     DAZKNO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.142"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..637)
                     /locus_tag="ACG8MY_002188"
     CDS             complement(113..637)
                     /locus_tag="ACG8MY_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0431357.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(630..1103)
                     /gene="tsaE"
                     /locus_tag="ACG8MY_002189"
     CDS             complement(630..1103)
                     /gene="tsaE"
                     /locus_tag="ACG8MY_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0431358.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1226..2206)
                     /gene="pta"
                     /locus_tag="ACG8MY_002190"
     CDS             complement(1226..2206)
                     /gene="pta"
                     /locus_tag="ACG8MY_002190"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0431359.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2359..3039)
                     /locus_tag="ACG8MY_002191"
     CDS             complement(2359..3039)
                     /locus_tag="ACG8MY_002191"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0431360.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3165..4034
                     /locus_tag="ACG8MY_002192"
     CDS             3165..4034
                     /locus_tag="ACG8MY_002192"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0431361.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4060..4575
                     /locus_tag="ACG8MY_002193"
     CDS             4060..4575
                     /locus_tag="ACG8MY_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0431362.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4622..5185)
                     /locus_tag="ACG8MY_002194"
     CDS             complement(4622..5185)
                     /locus_tag="ACG8MY_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0431363.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5240..5419)
                     /locus_tag="ACG8MY_002195"
     CDS             complement(5240..5419)
                     /locus_tag="ACG8MY_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431364.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5496..6005)
                     /locus_tag="ACG8MY_002196"
     CDS             complement(5496..6005)
                     /locus_tag="ACG8MY_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431365.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6101..6481)
                     /locus_tag="ACG8MY_002197"
     CDS             complement(6101..6481)
                     /locus_tag="ACG8MY_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0431366.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         1891 a         1409 c         1178 g         2207 t
ORIGIN      
        1 acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc ccttcgtgtg
       61 tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc ttctattcaa
      121 tcaaataact cattttatat acatctaaaa attcacctag atctgttctt gcccctctag
      181 gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct ctttcattac
      241 gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc caactgatga
      301 tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga atactgatac
      361 cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat gctgttccaa
      421 taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg tatatataat
      481 ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc aaaactaaaa
      541 attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca tctcctggca
      601 ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca tttttctaga
      661 attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac ataccgcttt
      721 tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg cagtgcttct
      781 tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata ttcatctaac
      841 cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa cggaatccga
      901 ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg cgttatccct
      961 aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc accagtcaat
     1021 atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg ttctgtcatt
     1081 gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct ttttgccagt
     1141 atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca gcagctgctg
     1201 tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct gtgataatcg
     1261 ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc ggtttgttca
     1321 agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca attttatagc
     1381 cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca gctacttctg
     1441 aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat tgcaattcgc
     1501 cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca actactttgt
     1561 ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct acttttggtt
     1621 cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc aattcttgcg
     1681 cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga tctcttccac
     1741 gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg atgatctgaa
     1801 gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt ccgtctgcaa
     1861 tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc ttttctgctt
     1921 gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt tcatcccact
     1981 tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct ctggcatgcg
     2041 ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt tcttctgctt
     2101 tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa acgattttga
     2161 tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga tgacctccag
     2221 acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt attttatctg
     2281 tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac tgcactataa
     2341 caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg gttcttctcc
     2401 taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa agccacgact
     2461 tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct tatcgatcat
     2521 tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt tttcccgttc
     2581 atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc gatgagaata
     2641 cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct gtttagccca
     2701 tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa gctctttata
     2761 gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag ataagccatg
     2821 agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa cttcttcata
     2881 aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct tttgggacgc
     2941 atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa attctgagga
     3001 caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct cctaataaaa
     3061 agaataactc acattttctt caatcacaag ggacaatctt caccctcagc acttgattaa
     3121 tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc aaattaattg
     3181 catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct gaaaatgccg
     3241 aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact ggtcgaaact
     3301 atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg atcactttga
     3361 acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg attccaaata
     3421 atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat gaagtagcaa
     3481 ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt gcttccatgg
     3541 tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc tcagctaagc
     3601 tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa gaaaagaatc
     3661 tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta ggtccagttg
     3721 gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa aacaatatcg
     3781 aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct cacgagcgtg
     3841 ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc agcatgctgc
     3901 aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa gattatgcca
     3961 aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg cgcgcgattg
     4021 cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt tttcattcag
     4081 gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg acattccctg
     4141 tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta tgccatgaat
     4201 ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg ttttgagctg
     4261 tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc cggtgacttt
     4321 tattatattc gtagacttca ctggacagtc caaggtgata tctataatca tcaatatcaa
     4381 atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt ttcaggagat
     4441 tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg tattgctgcg
     4501 gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt ttcttaccga
     4561 cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca ggtacatctt
     4621 tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc tgaccgacaa
     4681 ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca tccacactta
     4741 gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct aataatcgcg
     4801 aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa taccattcgt
     4861 tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga tatttctcta
     4921 agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa ccgtaagcaa
     4981 ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg taacgataat
     5041 ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct gtctgcttca
     5101 aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt tctggagcat
     5161 cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct aacatgcaga
     5221 aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta atgaaataac
     5281 catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact ggcatttctt
     5341 tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt ccgcaattag
     5401 ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc ttagcttact
     5461 cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca tacctgacgg
     5521 tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt gactagaagt
     5581 gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt catcgctgct
     5641 tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc ggccttttcc
     5701 gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac ttgtccttca
     5761 caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt ttcaactgca
     5821 aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc tgctgctttt
     5881 ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc ggcattgagc
     5941 agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa catcattttt
     6001 ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga acgaatgaaa
     6061 aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc agttctaatg
     6121 gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta tcttctctaa
     6181 tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca atatcgtcta
     6241 ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt tgtggcgcat
     6301 tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat ttatagtcat
     6361 ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta acataaaaat
     6421 cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt gtaaggttca
     6481 tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag ttccattgta
     6541 aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa ggcaaaaaaa
     6601 acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat gaaagtaaga
     6661 aattatattt tcaagaaaac aaaca
//
LOCUS       DAZKNO010000141         6568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000141 DAZKNO010000000
VERSION     DAZKNO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.143"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..446)
                     /locus_tag="ACG8MY_002198"
     CDS             complement(<1..446)
                     /locus_tag="ACG8MY_002198"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0431367.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQ"
     gene            complement(443..1258)
                     /locus_tag="ACG8MY_002199"
     CDS             complement(443..1258)
                     /locus_tag="ACG8MY_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0431368.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(1255..2079)
                     /locus_tag="ACG8MY_002200"
     CDS             complement(1255..2079)
                     /locus_tag="ACG8MY_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0431369.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(2069..3169)
                     /locus_tag="ACG8MY_002201"
     CDS             complement(2069..3169)
                     /locus_tag="ACG8MY_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0431370.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            3347..4132
                     /locus_tag="ACG8MY_002202"
     CDS             3347..4132
                     /locus_tag="ACG8MY_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0431371.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            4165..4548
                     /locus_tag="ACG8MY_002203"
     CDS             4165..4548
                     /locus_tag="ACG8MY_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0431372.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            4624..4755
                     /locus_tag="ACG8MY_002204"
     CDS             4624..4755
                     /locus_tag="ACG8MY_002204"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0431373.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            4724..4960
                     /locus_tag="ACG8MY_002205"
     CDS             4724..4960
                     /locus_tag="ACG8MY_002205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0431374.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            4972..5058
                     /locus_tag="ACG8MY_002206"
                     /pseudo
     CDS             4972..5058
                     /locus_tag="ACG8MY_002206"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(5038..6009)
                     /locus_tag="ACG8MY_002207"
     CDS             complement(5038..6009)
                     /locus_tag="ACG8MY_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0431375.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         1907 a         1505 c         1308 g         1848 t
ORIGIN      
        1 atctggttgt taagactaag atagacaatc ggcaacgccg taataaacaa tgcacctacc
       61 aacactacaa acattgggat accagtttca gcatacgttc gaagcaaagc cgttaccaaa
      121 atcactcctg gtaaagcata cggcagcagg agcagctttt ccagccaacg attcaaacga
      181 gggaaataca aatgaatcca aataatggtg ggaatcatca acaacagaat cgtcaccaag
      241 accacacctg ctaataaaag tgagcgaccc accgcagcaa taaattccct gtccgtcagc
      301 agctgttgga accattgcaa ggtaaaatca ctaggtaaaa gcgattttga ccatttagtg
      361 gagctggcat acaaaatcgt tccaataatc ggcagcaagt aatacaggac aaagcagaac
      421 aaaatcggtt ttattaactt tttcacgtct gcaccacctt gcctttcttt tgaataatca
      481 acattgccgt taccagcagg acacctaaag cgactgccaa cgcactgcct aaattaggct
      541 tggcgaacac atctcccgac actaaagccg caattcgaat cgttaaaaga tttacattac
      601 tgctggtcag ggcatacgct gtttcatagg tccccatgcc attagcaaat aagatgacaa
      661 aggtgctggc aatcgatggt cgcaaaaaag gcaaagccac cttgaaccaa gcatacgctg
      721 atgaagcgcc cagcaattgt gcggactcca gccattcctt tttcacttcc ttcatgctgg
      781 gatacaaaaa caagacccct aacggaattt ggaaataggt atacgtgatg gtcaatcccc
      841 accaagaata caaattgaag tccgctagaa atggcaaaat cattttcagc acacctacat
      901 tccccaacaa aataataaaa gagaatgcca gcggaatccc agcaaaatta gctgccaaat
      961 tggaaacaaa ggtgattttc tcctgcaatt tagtcgatag attcattaag cataaacata
     1021 aaataaacgt cccgattaac ccaatcacac tggagctgac agcaatcagt acgctgttaa
     1081 caaaggcctg tgtgtaatat ttgttgctga aaatatcact gtaattggcc aatgtccact
     1141 ggttagttaa atcactgtgc aagctgcaca gaaccataaa aagcagcggc agcagtaaaa
     1201 atgcacagaa gataaaggag gcaggaacca gaaccaagcg ttttttcatc tctttcatcc
     1261 cctcatcaat tcttgaatgt cctgcatttt ctctgacggt gcaattccta ataaccagca
     1321 aaacaatgga gccgccagca gctgtggtac agtttgttcc ttcagctttt ggcgtttatt
     1381 tgaaatcaaa taaaaaggaa cttcacgatg tgcagccaag ctgccgccat gcagtccgtg
     1441 cgcatccatc ccgtgatccg cagtcaccac gacttgataa cctttttgca gccattcagg
     1501 aaggtaaata gccaataatc tgtccgcctg attaaccgct gctgcatatt catgagaatc
     1561 ggcggtaaat ttgtgaccga catcatcaat attcatcgaa tgtacgacca aaaagtctgg
     1621 ctgatactga ttcactaaat aatgggcatc cgcaaataaa tgcgaatcag ggtaagtgtc
     1681 ctcactataa aagatgccat gctgaatcag tcgctcctcc tgatgctgga tgcgatcaac
     1741 caaacgatta tagggggctt gattgtacaa ctcgctgtac cagtggtaag cggcggctcc
     1801 tgtttttccg ccagcttcct taactaattc aaacagcgac cactctttgg atcgctgagc
     1861 atggtaatta ctatagatgc cattttcata ggctggaacg ccggtcatca gtacttcgta
     1921 taacggacgt gaattgctgg gcagctcact ttttaccttc aataagcttg cttgctggtg
     1981 ctcaactaaa tgattcatga acccaagctg ggcgacggct gtgtcgtagc gacagccgtc
     2041 tagtataact acaatcagtt tgtcaagtct actttgcata ggtcagtacc tccgcctgcc
     2101 aaagctctgg aagctctaca gaggtcttgt tccaagcttc attgtcttca actggcaccg
     2161 ccgcttcata agcatcctcc ggcagtaatt tttcagctac ttctttaggc agctctacct
     2221 tgcgaatagg tcgcgcatag ccctttgcca aattgatttg cccttcatca gataaaatat
     2281 attcccgtgc tagctttgcc gcattgggat gaggggcatt tttattgatt aaggttgtat
     2341 acccgctaat gatggagccg tctgttggca cagttacctc aaaacgctcg gagtcaattt
     2401 ggtcacgata atttagtcca ttgaagtccc aaacaactgc cacatcgatt tcaccttttt
     2461 caagatttgc tacgctggca tcaacggtgg acaaacggcc tttttctgcc atttttttgt
     2521 agtaattcag acctggctga atatcctttt cgtcacctcc gttggcaatc gccgcagcca
     2581 atacagcaaa ttgtgcttgg ttggcaatcg tcacatcccc cacgctaact tgatagtcac
     2641 cctcttgcag ctccttccag ctagtcggtg cttgcttcac attctcttta tcggtgataa
     2701 aggaaatggt tcccgtatag cttaacaacc aatccccatc atcatccttt gcccaatccg
     2761 gaatttcctc ccaatagttg gtcttataag gtaaggttaa ctctttgctt tcagccaacg
     2821 gaccgaagct gataccgaca tcaccaatat cggcggttcc attttttcct tctgattcaa
     2881 acttagccaa ttcttctgca ctggacatat ccgtatccac gtgacttaag ccgtattctt
     2941 tttcaaggtc gttccaagtg cctacccaat ttgcccaagt atccggcatt cccaagctgg
     3001 caatctctcc ttcttccttg gctttttcta taatcgtatc taaagagtcg tctgccgcag
     3061 ccgattgctt cgttgcttca cctgagccgc agcctgtcag caggatactt actgttgctg
     3121 cacaaaataa accgatagtc atgttacgaa ttttttcatt cattttcatt cattcttctc
     3181 ccctttcgat aattccattc taacaaagct ttgtaatgag agaatcagtt tcttgaaaag
     3241 gtttcatgaa caatgtaaag gttgtatatg gtttgtaaat ttcgacgaat tcccgttaac
     3301 cttctaaagc ttttgcggta tgatagtaga aaaaaggagg ctccctatgc agtattatga
     3361 tcagcatctc cacacgtatt tttcctttga ttcagaggaa aaatttgaaa actatttagc
     3421 ctatcagccg gagtttttcg tttctacgga tcactttgat ttaaacaatc catctaccgg
     3481 aaaagatgac attccggatt atccagccta tataaacaaa ctggactctt taaaagaaac
     3541 ctattccacc aaatttctgc gaggtatcga gattggcgtt gttccaggac aagaagagcg
     3601 gatcgtggat tacctcgctc agcatcctta tgatttaaag ctggtaagta ttcatcaaaa
     3661 tggccgcttt gattatatgg acgatgtggt tttaaccaaa gatcccattc aaactaccaa
     3721 ggaatatttt gatcagatgt tgcaggtctt gacggatttt ccagaaggag atattttgac
     3781 tcactttgat tatggattac gccgcttcac cttcacagcg gaagaattcg cggagcattt
     3841 tgaggctcag ctgctaacga ttttcaaaaa ggttgttgaa cgagaattgg cgatggagtt
     3901 aaatgccaag agcttcttga aatacggcaa cgaagacctt taccgttatg ccgttccttt
     3961 gtatcaaagt gtcggcggaa agctctttac cttaggctcg gatgctcatg ttgccgaaga
     4021 ttatcagctg ggctttaaaa aaatgagtca gctgttggca gactgtcagg tagaaaagtt
     4081 gctagtcatt caaggaaaag aacgccgcct gacgccgctg ccgattttgt gattcctata
     4141 taatagaaga aagtggtgaa aaaaatgagc aacctgtggg actacaatca agaggcaccc
     4201 atccattatt taattgcgcg acattgggat gcgttgaaaa ttgaggcggt ttgccgatcc
     4261 ttgttagcag ccgttccaaa acaacaactg gaaaattttt tggtggctga cagcttgcag
     4321 cgggaaaaag ttcaggctta ttttgcagcc tttaaagatc agcctttgga atatttgcat
     4381 gcgcagtttc atctttttta tcaagtagcc gccccagatg actacaacga tttacgagga
     4441 cagctgcaat tgacctttca agcggatgaa accgcctata ccgttttatt gggaatggct
     4501 cgccttggtg atcaggcgaa ggtcgagtgg cggattttcg atatttgatg gcaggatcaa
     4561 ggcgtttcag ttgcaaacct tcctctcttt caattagaaa aaccgcagca ggaagaagtt
     4621 tgtgtgctgg tcaccgatgc tggtgaaatt cgtctccgct tatttcctga ccaagctccc
     4681 ctggctgtag ccaactggcg ctccctagct gagcaaggcg tttatgacaa cacacctttc
     4741 ggctcgtgtc attaaggaat ttgttattca aggtggcgct ttggatggtt ctggtgatga
     4801 agctgtttcc agctatgagg gctttttcgc tgatgaagtg catcgcggac tttatcattt
     4861 taatggcgcc ttggccttag gggaccacgg cccccacacc aacggcaacc agttttttat
     4921 tgtgcaaaac acaaaggcgc aggctgacct gctaatgtaa agcaagcata cttggagctt
     4981 ggaggactac cggagctgga tggtcgttat actgtttttg gacaggtata ccaagggtta
     5041 gaaatcgtag cagcgatcgc agctcagccg acaaacagtg aagatgtacc gattgagcca
     5101 gtgttcattc gacaaattaa cttcgaaaaa acatcgtaaa aagacactca ctgacttcag
     5161 caaaagctct attcttgagc tggctgcagc tagtgagtgt ctttcattta tccataagat
     5221 aaagcttgga ttcaataaat aaataaatgt tttggttaaa gctgtgacca gcaaatgagg
     5281 aaccgtgcga gccagttgat ctgccaatgc aaacatttct ttaggtgaag gcgctggcgt
     5341 gtctgccaac ggatactcca gtcccaatgc ttggtaaccg tgcataccat agcagtggta
     5401 cggtagtaat tctaacggta cgaaccgatc aagatctgcc acaaaacaag caatcgtcag
     5461 cagctcggtc aggttcatat tgatacctgg aaccacagga actcgaattc gcacttcgca
     5521 gttaggatac tcaattaaaa agcgacgaat attcttcaaa atttgttggt tatctaagcc
     5581 ggtccattgt ttgtggagct tgctggtaaa catttttaca tccacaaaca atgtatccac
     5641 atagggagct accttttgga tttcattata agcaccacaa aagctggttt ccagtacggt
     5701 attgattcca atgtatttcg atttttgcaa aatttcttta gcaaaatccg cctgaaccag
     5761 tgcctcgcct ccgctaatgg tcactccgcc atccgagtga aagtaaaaaa cagcatcctt
     5821 ctcaatctca tccattacct cttcagcggt catggtctgg ccaaatcctt tttcaatctg
     5881 cttgctttgt gattcgggaa tcgaacacca ggcacagcgt aatgggcagc cctttaaata
     5941 aacaacggtc cgtaaaccag caccatcatg tagagaaccc tgttctattc ttaatactgt
     6001 tccttccata tagatttttg aattacacac tagtttaaac taaaacacac aaggaaaaac
     6061 ttaccattta atcataaaat tagctcatat aaatatacta ggatgtaaaa tagactggtc
     6121 aaaatctggt caaaaaagag gactaataca ggtagtcctc ttttttgtta ttttaatatt
     6181 ctaaatcatt ttttattggt ttattccctt attcgatgta gactcaaatc actatatcac
     6241 tagatatcaa acggcgtcat acggaatcaa aaacatcaaa tttgacctgt taaatggtca
     6301 ttgaataaca ttcaaaatca ataaaacgga gtcagacacg gagtcaaaaa cgctgagagt
     6361 aaaaaatata tacagccttg taggtaaagt accacagggc tgtatatttt ttatttcaga
     6421 ttaccttaaa tgattccatt tactttttat ctcatttttt cctactagtt agtatcaaat
     6481 actctcctat tttctcaata aactattttt ggatacatgg tatctgaaac agctaaaacc
     6541 ttgtcattac agcaaaaaat cacgttat
//
LOCUS       DAZKNO010000142         6535 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000142 DAZKNO010000000
VERSION     DAZKNO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.144"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..253)
                     /locus_tag="ACG8MY_002208"
     CDS             complement(<1..253)
                     /locus_tag="ACG8MY_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 family protein"
                     /protein_id="HFQ0431376.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQ"
     gene            complement(256..1152)
                     /locus_tag="ACG8MY_002209"
     CDS             complement(256..1152)
                     /locus_tag="ACG8MY_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0431377.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(1310..2152)
                     /locus_tag="ACG8MY_002210"
     CDS             complement(1310..2152)
                     /locus_tag="ACG8MY_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0431378.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            2284..2937
                     /locus_tag="ACG8MY_002211"
     CDS             2284..2937
                     /locus_tag="ACG8MY_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0431379.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(3000..3527)
                     /locus_tag="ACG8MY_002212"
     CDS             complement(3000..3527)
                     /locus_tag="ACG8MY_002212"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0431380.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3547..4494)
                     /locus_tag="ACG8MY_002213"
     CDS             complement(3547..4494)
                     /locus_tag="ACG8MY_002213"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0431381.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(4506..6389)
                     /locus_tag="ACG8MY_002214"
     CDS             complement(4506..6389)
                     /locus_tag="ACG8MY_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0431382.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
BASE COUNT         1685 a         1337 c         1145 g         2368 t
ORIGIN      
        1 tttgatactc actaaggaaa aagctgatga tttgattgac tttttcttta tttgaattga
       61 tcgttaagac aggatctcct tgtctttccg taagagctgg tatttctggg agttctggtt
      121 cccgattagc aaaaatacgg ctgcctaaaa atacggtcga accaagtact accccaagaa
      181 ggataaggaa ggcgaatttc cagtaatttg ttttctttgt tttgtttgaa gatacgtcag
      241 atcttgtttt catgtttatt gatcatcctc cttctctaac cattggtttt gtgtctcgat
      301 cagctgatct ctcactgcat ttgccatcaa ttgataacct aaattatttg gatggaaatg
      361 atcttcttca tagagcacat tatttttgat gccatttaga ttttctgtcg tgctagttgt
      421 ttgatctgtt ccttcttctg tgacacctac ttcctgattc aatccttgat aaagcaaatc
      481 attgattggg ataaagtagc agttcggcat tttttttgtc accgtttctg ttcctttgtt
      541 ccaattgtta acaattgtct gcatgtcagt gatctcagga aagttcaaat aaaatggatt
      601 gtagatgcct aaaatgtaaa taggtgcggt tttattgagg gatcgaaatt cttccaatag
      661 ttctgtgaca ttttcctgat agtttttcag tggcttttta aaggaattga ccgttaaccc
      721 gaataaatta ttttggataa ctttcatcaa atcattaccg ccaacagtca gtgtgataaa
      781 atcagctgtt ttgatattgt ttcgaatttc ttcttttgtt tttactcgct ttaagatctg
      841 gtcacttcgt tcaccattta caccatagtt ttctgtttca accgcggtca aattataacg
      901 gtcttttaga tcatttgcga caaggggaac gaatcctcct tgcttggttt gatctcctac
      961 tccttcagtc agcgaatctc cgatagctat gtagtgaagt ttttcttttt tatttggatc
     1021 ttctctcaca gtagttactg gtttcattag tgggccagct ttcggtatag aagcagctaa
     1081 acctgcatag atgatgatcg caattaataa tgggaacaaa acgaggatca aatgtcgttt
     1141 cgttaatttc attttttcac ctcttgtatt cgattggttc taatgttatc aaaaatttta
     1201 tcaaaaaggt agtctttatg aaaaaagaaa acaattattt tcctaatttt ataaagaaaa
     1261 agagaataag ctgcaagcct ttcaggctga gggcttatcc tcttttcgac taatctacat
     1321 aatacatgat tgcaaacgcg tctttaccag tatgcgtagc gataaccgga ttcgtatgca
     1381 gtacagggat atgcatgtcc gggaataatt cttgcaaacc ctctttgaat tcttgcgcca
     1441 attcttttgc atccgcatgt gaaataccga tttgtcgcac atttggcaat tctttcagtt
     1501 catttttcaa attattaaac catttatcga acgttttctt tcctcgcccc ttagttacag
     1561 ggattaattc agaatgggcg aaattcatca ccacgcgtat gttcaaaaca ctagacagca
     1621 aaccagttgt tcggctgatt cttccacctt ttaccaagtt atctagtgtg gatattccga
     1681 tgtataattt tgtattgtct tgtacttttt tgacttcttc aaggattttc tctactgttt
     1741 taccttcttt tgctaattca gctgctcgaa ttacctgaaa agataatcct tggtcagtgg
     1801 tatcactgtc gataaccgtt actttcgttt ttgttagatt actggcttgt cttgctgctt
     1861 ctactgttcc actcaagcct tttgtcatat ggatcgaaat cacttcactg ccgtcttctc
     1921 ctagctcatc atacatcgag atgaattctc caattggtgg ttggcttgtt ttgggcagat
     1981 tttttgcttt cgccatcatt tccataaatc tttcgccgtc tagttggtca tcatcgggat
     2041 atactgttcc gtcgatcatg atggatagcg gcatgacacg aatgcctaaa gcatcgcgta
     2101 catttttttc catcgtacat gaagaatctg ttacgatttt tacatttgtc attattttct
     2161 ctctctcttt ctttaacaag atgccttatt catgttaaac tacatgtaag acaatttcta
     2221 tttttagtat aacagacaaa taatgatttt tcattaatta cacctagtaa aggagccgtt
     2281 ttcatggaaa aaacgaaatt taatcgaacg tatgaaatat taaatgaagt gtttaatgca
     2341 gtcacccatg gaattgggac tggattgagt atcgcgggac tagtggttct tttagtaaaa
     2401 ggtgcccact tgcattcctc cctccatatt gtctcttatg ctatctatgg atcgatgatg
     2461 attcttttat ttttaacgtc tactttgttt cattcattga tttttacaaa agcaaaaaag
     2521 gtgttccaag tattcgatca tagttcgatt tttcttttga ttgcaggaag ttatactcct
     2581 ttttgtttgc tgagtatccg aggatggcta ggttggactt tatttgtatt aatctggttg
     2641 tttgcgattc ttggcattat atataaagcg ttgacgttgc ataaacggga gagagtatca
     2701 aaagtatcta cgatcatcta tatttttatg gggtggctct gtgtcgtggc tgctgttcct
     2761 ctttatcact ctcttggaat gactggtgtt attttgatgg tagcaggcgg gctttcttac
     2821 acgataggcg cattctttta tagcttgaat tctatccgct atatgcatgt tgtttggcat
     2881 ttgtttgtta tgttaggtgc aggttttatg tacttctctg ttttattctt tacttaataa
     2941 aaaaaataaa gctatggatg ggacagctct aaataacagc tagtctatcc atagcttttt
     3001 tatatcgtca attcgcctgt cccgcttttt acaggattct cattacgctg atacgtttca
     3061 aattgatgcg gataacgatt tttttcatcc atttctcctt gactagtatt gatcagtgac
     3121 caatcagacc agtcaacagg tggaaaataa gcatctccat cgaatacatg atggatgact
     3181 gtacggtata atatcgtaca aaatggcaga aattcttgat aaacagctga ccctccagcg
     3241 atgaaaaaga taccgtctgt ttctttttcc agagcaagca cgtcttccac actatgcatg
     3301 acatgcacgc cttctgcttt ataggaagaa tctctcgtca aaacaatcgt ctgacgattc
     3361 ggcaacggac ggctacccat cccttcgaat gtttttcgtc ccatcactag tgtatttgct
     3421 tctgtcattt gtttgaagaa tttcagatca tttggcaaac gccaaggaag acggtcttct
     3481 tttccaatca atccgttttc atcctgtgcc caaattgcag ctaacatttt ctcatccttt
     3541 ctgattctaa acagcgatag gagctttgat cgccggatgt gggtcatatc cttctaattg
     3601 gatatcttcc atctcaaaat caaatactga ctgcttttcc tgattcaaaa ctaaagtcgg
     3661 aaatgcgcgt atttctcggc tcaattgttc tttcatttgt tcgatatgat tgatataaag
     3721 atgagcatct cctaatgtgt gaacaaattc gccaaccgct aaacctgttt catgtgcaat
     3781 taggtgagtc agcaacgcat aacttgcaat gttgaacgga acgcctagaa acacatcccc
     3841 actgcgttga tacaattggc agcttagccg tccctcttga acataaaatt gaaacatcgt
     3901 atgacaagga ggcaaagcca tgctaggaac atcttccgga ttccatgcag aaacgatcaa
     3961 tcttctagaa tcaggagtct ttttgattgc ttcgatcact tgtttgattt gatcgataaa
     4021 gccaccgtct tttgtttccc agtgacgcca ttgatagccg tagatgtgtc caagatcgcc
     4081 gtatgtttca gcaaaggaat catcggttag gatcttctcg caaaaaagct tgctctgctt
     4141 tttatattct tcattgaatt caggatctgt caaacatcta tggccaaaat cagtcatatc
     4201 tggtccatta tagtctggac ttttgatgta gcgttcaaat gcccattcat cccaaatatg
     4261 gtttttattt tgtaatagat agcggatatt cgtatctcct tttaaaaacc aaagcaattc
     4321 gcttttgatc aatccaaaag gtactcgttt cgttgttaac aaaggaaacc ctttttgcag
     4381 atcaaaccgc atttggtatc cgaataagct taatgtgcct gtgcctgttc tgtcttcttt
     4441 acgatgccct tcttccaaca attttcttcc taagtcaaga tacgcttgtt ccatcctttt
     4501 tcctcctaat cttctaccca ttcactcaag tattcccacc gtgccatttt ttcttcaagt
     4561 tgtgcttctg tttcagatac gtcgttttgc aattcttgta atcgagtgaa attatctcct
     4621 tgatgattca tctcttcttg gagcaatgag attttttctt caagttctgc aatctcatct
     4681 tcaatcgttt cccattcttt ttgttccatg taagataatt tcttcttttc agcaggttcc
     4741 ttgctctgta tttttggttt cgctttttca gcaggctgct gggtctcttt ttgcttcagt
     4801 aaataactgg acatgtttcc gtaataaagt tcttgttttc cgtttccttg gaatactaat
     4861 aacttatctg ctaccttgtc caagaaataa cggtcatggg aaacagtgat cacagcacct
     4921 ggaaaacttc taaaataatc ttccaaaatc gtcaatgttg ctatgtccaa atcattggtc
     4981 ggttcatcca ataataaaac attaggttgc tgaatcagca gtttcaacaa aaataaccgg
     5041 cgtttttctc cgcctgaaag tttgcctatc agtgtcccat gcataaatct tggaaataaa
     5101 aatcgttcaa gtaattctgc tactccgatt tgactgccat ctgctgtttt tgcttcttca
     5161 gcagcttctt gtaagtaagc aatcatccgt ttatctggtg ccatttcttc attttcttgt
     5221 gtataatacg caagatgaac cgtttcaccg atagacattg taccgctatc caggggaatt
     5281 ctgccagcta gaatattaag caatgtcgat tttcccgcac catttttacc agtaatacct
     5341 agtctttccc gagattgaat caataaatct aaatgttcta gcagtgtttg gtcatttata
     5401 cggtaactgc catctttgat ttccagtact tttttcccta aacgttgtgt cgctaggttc
     5461 agctctaaat catcttcttg gtttacttgg tacaagttct cctttagatc ttcgaaacgg
     5521 ttgatccgtg cttgctgctt ggtgccacgt gcctgtacgc cagccctcat ccaagctaat
     5581 tcctgtttat aaagccgctt gcgcttttct tcttgttcta cttctacacg atcacgttct
     5641 gctttttcta agacgtaagc ttcataattt cctttatact cgtataattt tccaaatgaa
     5701 agttcgaaga ttcggtttgc tactctatct agaaaataac gatcgtgggt aaccatcaat
     5761 agtgcgccac ggtattgttt caaatagttt tccaaccact cgatcgcttc ataatctaaa
     5821 tggtttgttg gttcgtctag taatagtaaa tcgggttcat caatcaacac ttgcgccaga
     5881 ctaactcgtt ttttctgacc acctgataaa gtactgattt ttttatctaa ttcactgatt
     5941 cctaattttt gcaaaataat ctttgcgttg gtatctgtag tccaagcatc ttctttattc
     6001 atttgttctt ctgctttagc ataacgtttt tgcacatcag ggtcttctcc gttttcccct
     6061 agttcgatca atgcttcctc atatgctcga acagtttgga tcaaggggct gtttccttga
     6121 aagactgctt ggagaacagt taattcatcg gaaaattcag ctgcttgaga aagataacca
     6181 atacggtaat cagttggata aaacactgtt tgccgatcac cgtcgctgct atcgataccg
     6241 gcaataatat ttaagagact tgttttaccc gtcccgttcg tcccaatcaa cccgatacgg
     6301 tctttttcgt ggaccaaaaa agaaatctgg tcaaacaaat ctttttcacc ataagttttt
     6361 ttgaggtcag ttacttttaa ttctttcaac atgttccctc cttatcttgg atattgtatc
     6421 ataaaaatag tgaacttcag gaaaattttt ttggtatttg ttctttttgt gttctcaaaa
     6481 aaagaacagg accgattcga tcctgttctg atatgttttg ttttttataa aactt
//
LOCUS       DAZKNO010000143         6294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000143 DAZKNO010000000
VERSION     DAZKNO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.145"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(5..628)
                     /locus_tag="ACG8MY_002215"
     CDS             complement(5..628)
                     /locus_tag="ACG8MY_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431383.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(787..2184)
                     /locus_tag="ACG8MY_002216"
     CDS             complement(787..2184)
                     /locus_tag="ACG8MY_002216"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0431384.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHPEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIKF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2216..3187)
                     /locus_tag="ACG8MY_002217"
     CDS             complement(2216..3187)
                     /locus_tag="ACG8MY_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0431385.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKELLEKGVTAIFVSNYNMSIGAIECFNQEGIRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGEQAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3561..3986
                     /locus_tag="ACG8MY_002218"
     CDS             3561..3986
                     /locus_tag="ACG8MY_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431386.1"
                     /translation="MKKLIVLFILSAGLLTLTGCNLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQETDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATITFTKIGNYRLKDEDGNIYSL"
     gene            complement(4474..5175)
                     /locus_tag="ACG8MY_002219"
     CDS             complement(4474..5175)
                     /locus_tag="ACG8MY_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0431387.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5191..5631)
                     /locus_tag="ACG8MY_002220"
     CDS             complement(5191..5631)
                     /locus_tag="ACG8MY_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0431388.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5631..6206)
                     /locus_tag="ACG8MY_002221"
     CDS             complement(5631..6206)
                     /locus_tag="ACG8MY_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0431389.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         1784 a         1299 c          995 g         2216 t
ORIGIN      
        1 tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg
       61 cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc
      121 ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt
      181 tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga
      241 tcacttttcc atttttcaaa cgggctactt tgtcgccatc atgagtcaaa agcaaaacgg
      301 tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga
      361 tattaataaa ttgattttct tcttttcctt ttggcaaata gatgtccaat tcgccttttt
      421 cgtaattggc agttatttta tactccgccc accaataagt tgagaattct tttttgaaat
      481 tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg
      541 tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa
      601 agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat
      661 tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat
      721 tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat
      781 tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccagt gacccgattt
      841 tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt
      901 atatgcattg gtccatgacc agttatccat acatgtccac atatgatagc ctaaacaatt
      961 ggcaccttct tgaattgctt gatggacata tttcagatga gattggacaa atttgatccg
     1021 ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat
     1081 accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat
     1141 atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg
     1201 catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt
     1261 ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt
     1321 ttctttgatg accgtcaaat cctctggatg gatcacaggc atgagatcaa gttcttttac
     1381 tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt
     1441 gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg
     1501 atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta
     1561 agatttgatg gctaacgctg aggccaacgc ttcatgatag ccaacttgga tcgctttttt
     1621 catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat
     1681 tggttcatta tgagtaaacc atttttttac tttcgaaccg aacaattcaa aacaactttt
     1741 ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag
     1801 atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa
     1861 ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc
     1921 aggtatcaag cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat
     1981 caactgtaca tcctcttgat acttcgtata aacttgtgaa gttttttcag gtcctacttg
     2041 gtcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc
     2101 atcacctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt
     2161 tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt
     2221 tgattccatg tatgatattt tccatcacaa ttactttttc attttcttcc tcttcttttt
     2281 tgattcgttt caacagctgt tctgcggctt gttccccgat tttatctgtt ggtggtgtaa
     2341 tcgtgatttt cggaaggatg ctcaaatcgt ttttatccaa ataatcataa tgactaaacg
     2401 caatatcctt cccgattctg ataccttctt gattgaaaca ttcgatagca ccgatcgaca
     2461 tattataatt gctgacaaaa atcgctgtta ctcctttttc taataattct tttgccccat
     2521 aatagccact ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat
     2581 tttgtaccgc ttcttttgct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg
     2641 cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta
     2701 cactttctcg atcattcact aatattgtat caatacctgc tggtccttct gggcaattca
     2761 atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg
     2821 ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa
     2881 ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta
     2941 ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa
     3001 gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt
     3061 ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat
     3121 tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccacg tcacggatcg
     3181 atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta
     3241 tgatgaccgc tttctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt
     3301 cagtagagca acttcctctc actattaatt atacttaaac aagtatttga accggttcat
     3361 ttttaatata gcattttttt aaaagattgc aacacttttt ttaaacgctt tcctaaaatg
     3421 gcgaattttt tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg
     3481 tgcgtttttc tcttccatcc gtttgaagtt ttactttcca atggtataat ttctttatcc
     3541 tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac
     3601 ttttgacatt gactggctgt aatttgtttg gcggctctga ccaatcctcg gatgaaacaa
     3661 ccgattcttc tgtcttaaaa gcggaaaaag cagatatcac taacagtgta tatacgctaa
     3721 aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg
     3781 aatggacacg cacgtatcag acacaagaaa ctgattcttc tgaatcatct gaagaaaaaa
     3841 cagtgctgac agacatcaag attactacca aagatgacga atatattatt tcaggaaaag
     3901 aaaataaaaa agaagcaacc atcacattta ccaaaatcgg taattaccgt ttgaaggatg
     3961 aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct
     4021 tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat
     4081 agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa
     4141 ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt
     4201 gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa
     4261 actcactatg gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca
     4321 tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat
     4381 ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt
     4441 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
     4501 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
     4561 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
     4621 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
     4681 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
     4741 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
     4801 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
     4861 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
     4921 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
     4981 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
     5041 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
     5101 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
     5161 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
     5221 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
     5281 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
     5341 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
     5401 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     5461 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     5521 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     5581 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     5641 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     5701 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     5761 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     5821 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     5881 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     5941 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     6001 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     6061 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     6121 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     6181 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     6241 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtgg
//
LOCUS       DAZKNO010000144         6209 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000144 DAZKNO010000000
VERSION     DAZKNO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.147"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            290..646
                     /locus_tag="ACG8MY_002222"
     CDS             290..646
                     /locus_tag="ACG8MY_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0431390.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            656..1555
                     /locus_tag="ACG8MY_002223"
     CDS             656..1555
                     /locus_tag="ACG8MY_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0431391.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2075..2803
                     /locus_tag="ACG8MY_002224"
     CDS             2075..2803
                     /locus_tag="ACG8MY_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0431392.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            2948..4975
                     /locus_tag="ACG8MY_002225"
     CDS             2948..4975
                     /locus_tag="ACG8MY_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0431393.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5083..6180
                     /locus_tag="ACG8MY_002226"
     CDS             5083..6180
                     /locus_tag="ACG8MY_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0431394.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         2155 a         1013 c         1275 g         1766 t
ORIGIN      
        1 gtgaacaaaa agtaattctt tttccaatag ttgttaaagg ttttttattt tttatagtat
       61 tttttgttat ttaagaaagg aataaggtta aacaaataac aggttgcttt ttgggtcttt
      121 ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc tcttttgtga taagagaaat
      181 cataataaac gaatgactta ctttccaata aattgagaac agtcagaacc agacaaatta
      241 gttgcatctg atgaataaag tgattattct atgacatgag gtgatacaga tgaaattaga
      301 agtgacaaaa gaagcacaag aagtattgaa gaataaatta gaagcagacg atcaactttt
      361 attagatatc gaaaacggtg acggtccatt tgcagaaagc caagtcagtt gccaattgga
      421 tacggcattt cgattgatca ttgtcaaaaa agatacacaa acagatctaa gtctttattc
      481 tgtagtggct gatacaccag ttggaccaat caaaatcaaa gacagtgcaa tactttacat
      541 ggatgatcca tccacacttg ctttagaacc aacttataat agtttacaat taaaaggacc
      601 aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa gactagaggg ataaaatggg
      661 aaaattggcg atcatcagcg acctacatgt ggacatcaat aaattcggcg aatcagagct
      721 gatgcagctt tggcaagttt tgcaaaagca caagatcact cggcttcatc tagccggaga
      781 tacggctaat aaagtagacc gttgcctagc tgttgtcgaa tttttcgtca ataaaggatt
      841 atctacgaca tttcatttcg gcaatcacga gttggcagat gtgcccaatg aacagatgat
      901 ggaccatttt ccagacccac attttttaaa tgaacggtat attgctttaa atgagcagac
      961 cgttctttta gggatgaatg gctggtacga ctatcagttt tccgaattga aagattacga
     1021 ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg ttcatccagc gtgggaaaac
     1081 agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa aatttgctag accagctgga
     1141 agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt cctaaacggg catttatcat
     1201 gtatcaatct gctccttatg agcgttggaa caaattaaat gcatttttag gatcagaatc
     1261 attcggagaa ttacttgacc gttattcaaa tatcaaacaa gtagtttttg gccatacgca
     1321 tcgtcgcttt gaagataaat ccatcggtca cacgatttac agctgcagac cttttggtta
     1381 ttattatgaa tggcagctga cccgagattt tgtcttgaaa gaacagttgc tagacagcta
     1441 tgatcctatg aaattacgtg tcctcttgcg tgcccatcac ccagcttttc aggcctatca
     1501 aacacaacat ttgcaagagg aatttgaaca agcgatgaca atcgttccgt attgagacag
     1561 cattagtaat gtgtaaaaga aaataccttg taaaacataa ggataagtaa gagcagtaga
     1621 tccatttatc gtggatatgc tgcttttttt gcggaaaata atcagcgaat gtttatttga
     1681 tcggatgatt tgtaactcgc agagtactag atgcaagcgt gtattctttt tttgaacgat
     1741 aatcacaaaa aaaaatataa tatacatttt ttatttaatt ataattaata ttgattgaag
     1801 tctaggcagt caagtcacat tttgatagat aggaggggga cttacgaatt attttaaagg
     1861 ctatgacagc tgtatttcta gattgtgttt gcatcaagtt gcaataaaaa ctagaaatca
     1921 aatcgggctg ataatcattt ataactaatc gatacatagg accaaatata ggtctatgtt
     1981 cttttatttc ataagtatcg taaaacagaa ttttgctatg gattaatcca atcgactgat
     2041 atgtttagta aataaagaaa gaggaatgtc aatgatgaaa aaagaaatcg tattaacagc
     2101 agcaatgatg ttattcagca tggtagcaag tacgactttt gtttctgcaa ctgaagtata
     2161 cccaaaggaa tataacacag aaggaacgat tacatttgaa gcaggagatg agggtgtaac
     2221 tcctcctgtt gatccagaga atcctgatcc caataaacct gtcgatccat cagatcctcc
     2281 aagccctgga acaggagggg cattatcgat tgattatgga tcaaaattca aatttgggac
     2341 tcaaaaaatt tctactgcgg ataaaacgta ttatgcagct gctgatgtga tgaacgatgg
     2401 gtctagaaaa ccgacatatg tccaagtgac agataggaga agtaccttat ctgggtggaa
     2461 attaagtgtc tctcaaccag aacagtttaa aactgccagt ggggatgaac tagttggagc
     2521 acagctgaaa tttacgaaag ggcaggctgt ttctttggtt gatccaacat atacgccgca
     2581 aacggtgaat tctgaattaa cgttgacacc gggaggaaac aatacgttag caattaatgc
     2641 taaatcagga acaggtgtgg ggacttgggt ctatcgattt ggtgcaaatg aaaatgaaaa
     2701 tcaagatgcg gtacagcttt cggtacccgg taaatctgtc aagttagcgc aacaatattc
     2761 cactaaatta gtttggacat tagaagatac acctaacaac tagtttactg aaaagaactg
     2821 ttaaaaataa atacgtagtc atattgtctt gcgaggtaat cgagcaaaaa ttgtcaaagg
     2881 ctaggctaaa tttgtttgga tacgaatagt tcatctagga atttaacgat caggaggaaa
     2941 agatatattg aaaaagagga ggatcgtgct cattttaagc agtctcttag tgactaatat
     3001 ctttccgatc atagggtact ctttggaatt gaatcagttg gagcatggaa atagaagaga
     3061 cgaagaaagc aaactcactt cgacattcga cagtttaata gaatccaacc agacacaaga
     3121 aagcagtgag gatttaagta tttcgagtag tggagagacc actgacaacg tagaagctga
     3181 gggttcggac aaacaaggac tagaagatgc accaatttct gcggttgagc tgcctgacga
     3241 tcccaatatt atttctatcg ataaaatttt tcaaacacct atcggtacta gcacgtccat
     3301 attggaaaat gggaagttac tgcagttgaa cccagcagaa aggtctcaaa gaggagctat
     3361 ttggtcaaat aagaagatta gtttactctc ggattttaca tttaaatctt atttatactt
     3421 aggaaatcaa cgagatagag cgggagatgg catgaccttt acattgactg gcgatcctag
     3481 aatggctagt aatcccagtc aagttattgg atcgccaggt atgggaattg gctcatattc
     3541 tactcgttct ggccaacctt acattgcgaa tgcgctctcg attgagtttg atacgtataa
     3601 aaatagtggt tcttctgatc gaatggatcg agagataact gctgataata gagggcatgg
     3661 acatgtcgct tttgtcacgc ctaaaccaaa taataataat tattctggag agcatactgg
     3721 cgtgacggta gctcctacat ttctttctaa tggcacatgg cgcttgttaa taattaaatg
     3781 ggacgcggag caaagaagtc ttacttatga tttggaaggt gtcggtcact cttctcggat
     3841 tatacctgat ctcattaggc agtttggttc tacagaagta aattgggggt ttacttcttc
     3901 aacaggtact ttttttcaag aaaatgctgt cgctgtcacg cgacttccat cgaatgtcac
     3961 atcaacagct gatgtaaaag tcaacgatag tgattatggg gccatcgctg aagctggagg
     4021 aaacgataag ataactatca gaaatacatt gaaggtcgag ggggattttc ttgaatataa
     4081 gaaaatgcag actagtatcg acctcccaca agaattaact attcccgagc atatcttgat
     4141 caatggaaat caaattgatg ccaaagatat cttgataacc ggaaatcgag tcacgctaaa
     4201 tctgacagat tattttattg ctggagatac gttaataatc gaattggaga caactttgca
     4261 ggaagtaatt cctgaaaaag tactcactat gcattttgaa taccttgaaa acgggaattt
     4321 gattgagcaa tcgaatgaaa taaacctaaa gatcgcaaag ctgaaggaga tagatatcca
     4381 tgtttattat aaagataaag acagtcagat tgatattgct gaaatgaaaa caatcactgg
     4441 gaaaatagga gagaagtata aagaaactcc tattgatatt gaagggtatg tttttgactc
     4501 ggattcagcg aatacagaag gcatcttctc cgaaaataca gaggatatct atttttatta
     4561 cagaaaaggc aaactttatc tctcaactgc tcctcaatat gtcaattttg gaaaacataa
     4621 aatatccagt agaccattaa gtgaattcgg acagttttca ggtgggttga aaattataga
     4681 tgaacgggct tttggaagtt ggaaattaca attgaaacaa gagaaagttc ttacaaatgg
     4741 agattttgag cttccagaag ctctttcctt tgttacctct gaaaatacaa cggttatagg
     4801 gacatccgct gttacgattt tcgaaagtga tcaaaagggt gaaagcgatt tgtcctctct
     4861 tttagattct acagctcatc gtggaatcag aatagatatt cctgtagaaa accaaagaat
     4921 cggaaccttt gaggggaaac tatcttggat acttgcagat gtcccaggaa attaacagga
     4981 agagaagaat ataacaaaat tattgagttt caagaagggc acaggtacca taggctcttg
     5041 tgtcccacag agacaagatt ttgttatagt cttctcttac atatgattaa aggagcgatt
     5101 ttcttgagac caaaacaaca attatttata acactcctag ttattttagg ggcacttttt
     5161 aacaatatat cagtgtcagc tagtgcattt aatttttcag tgacgccgat aacttcagag
     5221 aatcaaatcg acaagagaaa aacttacttc gatcttcaac ttgatccgga tcaggaagta
     5281 gaagtaaaag cggaattgag aaatgataca gaaaaagaag tgaaaatcga tatttctgtg
     5341 aatagcgcaa ccaccaattc aaatgtgatg gttgaatacg gaaaaaacga gattgaaaaa
     5401 gacatgagcc tgatctttga tctggtagat tatgtgagct atccacagat agtgacgcta
     5461 aaacctaaaa gtgtccaaac cgttgctttt catgtcagga tgccgaatga gcgatttgat
     5521 ggtgtattgg ctggtgggat cacattccaa gaaatccaga cggaaaagga ccaaacagaa
     5581 actaaggatc aaagcttatc gattgagaat gaatacacct atattattgc tttgttgatg
     5641 caacagacac tgaacgaagt tgctccgaac cttactcttc atgaagttaa accagatcaa
     5701 atcaatgcaa gaaatgtcat cttagcaaac gttcaaaatg atcaaaaaac ctatatcaat
     5761 caagtagtta ttgaaacaaa aattacaaaa aaaggacatt ctgaagtttt atatcaagag
     5821 gaaaaagaag gattgcagat tgcgccgaat acaaattttt cttttccaac tgctttaaat
     5881 ggtcaaccgc taaccccagg tgaatatcac cttacaatga ctgttttagg aaatgaaaat
     5941 gaaaatggaa aattttcgag aaaaaaagga aatactacga tcaactatac caatcaatgg
     6001 gtatttgaaa aagattttac aatagacgga aaagtagcca aggaattgaa cacgacggat
     6061 gttacgatta aaaaagacaa taattggatt tatatcttag tagcttcact tttcttattg
     6121 tttattttat tatgcatact tttgtttgtt ttgagaaaaa aagaagagaa agaaaagtaa
     6181 ggttaagata acaaatagta aatttttat
//
LOCUS       DAZKNO010000145         6150 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000145 DAZKNO010000000
VERSION     DAZKNO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.148"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(151..1830)
                     /locus_tag="ACG8MY_002227"
     CDS             complement(151..1830)
                     /locus_tag="ACG8MY_002227"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0431395.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(1832..2044)
                     /locus_tag="ACG8MY_002228"
     CDS             complement(1832..2044)
                     /locus_tag="ACG8MY_002228"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0431396.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            2437..4167
                     /gene="efrA"
                     /locus_tag="ACG8MY_002229"
     CDS             2437..4167
                     /gene="efrA"
                     /locus_tag="ACG8MY_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0431397.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            4164..5924
                     /gene="efrB"
                     /locus_tag="ACG8MY_002230"
     CDS             4164..5924
                     /gene="efrB"
                     /locus_tag="ACG8MY_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0431398.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         1840 a         1104 c         1294 g         1912 t
ORIGIN      
        1 atttttttgt atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca
       61 aggaataaga acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca
      121 ggacaaggtg ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg
      181 caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat
      241 cgcttctttc aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg
      301 gaataagatt cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc
      361 acgagagaga atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac
      421 agctaataca agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat
      481 atcaccgatc ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg
      541 cgcagaatcg ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc
      601 aggaacacct acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg
      661 gacaggaata aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc
      721 accgtgaccg gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc
      781 agacagcaaa ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga
      841 aaagatcact gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg
      901 gctcaacgcg gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc
      961 aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc
     1021 taaacgttga ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg
     1081 accagttgcc acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct
     1141 ggcaaaaatg atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt
     1201 tttttctgat ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa
     1261 gactccttct tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt
     1321 gaaatcgaat ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat
     1381 ccctaatgca tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa
     1441 acggatgatc gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc
     1501 atctaatttg tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc
     1561 ttgtttcagc aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa
     1621 ggcttttact ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat
     1681 tccaagtaaa tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg
     1741 gaattgtacc ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc
     1801 tgtctcgtta tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat
     1861 tttctttttc gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg
     1921 gggtattttc ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag
     1981 attttgtttc ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga
     2041 tcattgtttt gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga
     2101 caacacaaaa cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga
     2161 ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta
     2221 ccttctgtat aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag
     2281 tatagaaatc aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa
     2341 tgactttttt atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa
     2401 tgaaaatgta acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc
     2461 atgcgttata agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt
     2521 gaattaggac ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat
     2581 tttgattatg tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt
     2641 ttgaatatct tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt
     2701 cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc
     2761 gtatcttcat tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa
     2821 aatattttac gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc
     2881 atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg
     2941 gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta
     3001 gataaaatca acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc
     3061 gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt
     3121 tcaaaaagct tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat
     3181 atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt
     3241 gtaggagatt tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta
     3301 tttgctagtg tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa
     3361 gccctagatg ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact
     3421 aaagggtata tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca
     3481 gtgatacgaa atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt
     3541 accggtagcg gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa
     3601 ggaaagatct tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat
     3661 aagattgggt ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg
     3721 cgatacggga aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa
     3781 gcttcagaat tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt
     3841 tccaattttt caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt
     3901 ccggaaatct atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag
     3961 ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt
     4021 gtgggaacga tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt
     4081 atggggacgc atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct
     4141 caattatcaa aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc
     4201 gatatatcag accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag
     4261 tggctttcaa cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta
     4321 atattgccaa tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca
     4381 cagttgttgg aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg
     4441 tcgttcaaag tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc
     4501 cggtttcata tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg
     4561 tagatgcagt cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg
     4621 gaattacgtt ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta
     4681 tgatcatgat tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat
     4741 attttcaaag catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga
     4801 caggttttag tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa
     4861 aagttaatca cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga
     4921 tgcctctagt tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct
     4981 tttatgttat tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt
     5041 ggcaaatcag tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat
     5101 cagcggcaac gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag
     5161 tagatgcttc actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg
     5221 catatgatcc gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa
     5281 ccgtagctgt ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc
     5341 gtttttatga cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga
     5401 gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag
     5461 gaacgattgc agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg
     5521 atgctgcgaa aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata
     5581 tggccatcaa ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg
     5641 cccgagcagt ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag
     5701 acacccgttt agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa
     5761 gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg
     5821 atcaaggtca gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt
     5881 atgaaaaact gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa
     5941 acagtttaaa acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag
     6001 aaaagtgagg acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga
     6061 gaagttgatc gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt
     6121 gaaaatgagt ttcaaatcga acgcttggca
//
LOCUS       DAZKNO010000146         6094 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000146 DAZKNO010000000
VERSION     DAZKNO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.149"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..2662
                     /gene="adhE"
                     /locus_tag="ACG8MY_002231"
                     /gene_synonym="adhC"
     CDS             65..2662
                     /gene="adhE"
                     /locus_tag="ACG8MY_002231"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0431399.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2891..3997
                     /locus_tag="ACG8MY_002232"
     CDS             2891..3997
                     /locus_tag="ACG8MY_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0431400.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4014..5038
                     /locus_tag="ACG8MY_002233"
                     /pseudo
     CDS             4014..5038
                     /locus_tag="ACG8MY_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5044..5523
                     /locus_tag="ACG8MY_002234"
     CDS             5044..5523
                     /locus_tag="ACG8MY_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0431401.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5539..>6094
                     /locus_tag="ACG8MY_002235"
     CDS             5539..>6094
                     /locus_tag="ACG8MY_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFQ0431402.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         1961 a         1076 c         1372 g         1685 t
ORIGIN      
        1 aaatattgta tgatatacat gtgaattatt taacaaataa ccatgaccca ttaggaggat
       61 aatgatggct aaagtaatag aaaaagaaaa aacgaagaca attgacgtac aagcaatgat
      121 tgatgatttg gcagaaaaag caaatgcagc attgaaagaa atggaaagct ttgatcaaga
      181 aaaagtagac catatcgtgc atgaaatggc aatggcagct ttggatcaac atatgccatt
      241 agcgaagatg gctgtagagg aaacaggccg cggtgtttac gaagataaag caatcaaaaa
      301 tatgtatgcg tccgaatata tttggcataa tatcaaacat gataaaacag ttggtgtgat
      361 caatgaagat gtccaaaaag ggttgattga aattgctgaa ccagtcggtg tggtttgcgg
      421 agtaactcca acaacgaatc cgacttccac aacgattttc aaatcaatga tcgcgttgaa
      481 aacaagaaat ccaatcgttt ttgctttcca tccaagtgcc caaaaatctt ctgcagaagc
      541 agcacgagtg gtacgtgatg ctgctatcgc tgccggagct cctgaaaatt gtatccaatg
      601 gatcgaacat ccttcgatcg aagcgacttc tatgctaatg aaccatccag gtatcgcgat
      661 cgtcttagcg acaggtggag ctggaatggt caagtctgct tactctacag gaaaaccagc
      721 tttaggtgta ggtccaggta acgtaccagc ttatattgaa aaaacagcaa aaattaaacg
      781 tgcagtgaac gatttgatcg tttcgaaaac atttgataat ggaatgattt gtgcttctga
      841 acaagctgtg atcgtggata aagaaatcta tgcagcagta aaagcagaat tccaagcaca
      901 ccaagtttat atcgtaaaac cagatgaatt gcaaaaatta gaagatgcag tgatgaacga
      961 aggaaaatat gcggtcaatc catcaatcgt tggacattct gcgatggaaa tcgcaaaatt
     1021 ggcaggaatc agtgtaccaa aaggaacgaa aatgctgatt gctgaattgg aaggcgtagg
     1081 cccagattat ccgctatcaa gagaaaaact ttctccagtt cttgccatga tcaaagctaa
     1141 caacacggac catgcgtttg atttgtgtga aggtatgttg aatcttggag gattaggaca
     1201 tacagcagtt atccattcag aaaacgaaga gttgcatgtc aaattcggtc ttcgaatgaa
     1261 agcttgtcgt attttagtga atacaccatc tgcagaaggc ggcatcggtg atatctataa
     1321 cgaaatgatt ccgtcattga ctttgggatg cggttcttat ggtaagaact ctgtttctcg
     1381 taacgtctct gctgtaaact taatcaatgt caaaactgta gcgaaacgga gaaataacat
     1441 gcaatggttc aaattaccgc caaaaatctt ctttgaaaag aactctctat tgtacttaga
     1501 gaaaatggaa aacgttgaac gtgtcatgat cgtttgtgac cctggtatgg tccaattcgg
     1561 ttactgcgat acagttcgtg aagtattggc acgacgtaaa aatgatgtga agatcgaagt
     1621 cttctcagat gttgaaccga acccatcgac aaataccgta tacgcaggaa cgaaactgat
     1681 ggcagacttc aagccagata cagtaatcgc tcttggaggc ggttctgcaa tggacgcagc
     1741 taagggaatg tggatgttct atgaacaccc agacacatct ttctttggtg caaaacaaaa
     1801 attcttggat atccgtaaac ggacatataa gatcgacaaa ccagaaaaaa cacaattcgt
     1861 atgtatcccg acaacatcag ggactggttc tgaagtaacg ccatttgcgg tcatcactga
     1921 cagtgaaaca catgtgaaat atccgttagc agattatgca ttgacgccag atgtcgcaat
     1981 cgtcgatcct caattcgtca tgtcagttcc agcatctgtc acagcagata ccgggatgga
     2041 tgtgttaaca catgcaatcg aatcctatgt ttcagtcatg gcttcagatt atacacgtgg
     2101 attgagccta caagcaatca agttagtctt tgactattta gaaaaatcag taaaaacacc
     2161 tgatatggaa tcacgtgaaa aaatgcacaa cgcttctaca atggctggta tggcgtttgc
     2221 taacgcattc ctaggtattt gtcactcagt cgcacataaa atcggcgggg aatacgggat
     2281 ccctcatgga cggacaaatg cgattctttt accgcatatt atccgctata atgcaaaaga
     2341 tccacaaaaa catgcgatgt tccctaaata tgactatttc cgtgcagata cagattacgc
     2401 agatatcgca aaattcttag gtctgaaagg cgaaacaaca gaggaattag tcgaagcttt
     2461 agcaacggct gtttatgaat taggaaaatc tgtcggcatc aacatgagtc taaaagctca
     2521 aggagtcaca caagaaacac tagatacaac cgtagaccgt atggctgaat tagcttacga
     2581 agaccaatgt acgacagcta atccgaaaga accattgatc tctgaattga aacagatcat
     2641 cattgatgct tacaacggat aagcaaaaga aagcgtagaa aaaagataag aaaatagatg
     2701 aaaaagaact tggaaaacgt tgtttctcca agttcttttt ttaatttcca agtaactgaa
     2761 agttactttt gttatttaac atttaggtca attttagaca gtttttgttc aaaaagcgat
     2821 atttatagtg gacggtatta ctgctatatt aaggatataa aatgaaagcg tttaaaaagg
     2881 tggcgtaaag atgattatta aagaagagat ttttgtacct aaagaattag tgaaagaaaa
     2941 aattgacctg ctcgttgaaa acctgacaac gatcaaagac gataatggtg aatttttgtt
     3001 ggactttgat ggactgaaag tagatgacaa aagctggaca gtgtggaatt ggccacaagg
     3061 tgtcggtcta tatggtattt ataaaaatta ccagatgaca aaaaatcctc gcgcttatca
     3121 agtggtcaat gaatggtttg aggatcgtat ggaagaaggc gctccgccca aaaacgtcaa
     3181 tacaatggca ccgctattga ctatggctta cttgtatgaa gacacgaaag attcacgcta
     3241 tttgccttat ttagaacaat gggcagaatg ggtcatgaat gacatgcctc ggacaaatga
     3301 agacgggtta cagcatgcca catatggacc ggaaaataag aatcagttgt gggacgatac
     3361 attgatgatg acagtgcttc ctttggcaaa aatcgggaaa ctgttgaatc gcccagaata
     3421 tttagaagaa gctcgttacc agtttatgat ccatatcaaa tacttgatgg ataaacgaac
     3481 aggattgtgg tatcacggct ggacgtttga aggaaatcat aactatgccg aagcattttg
     3541 ggctcgtgga aactgctggt tgacgattgc tattccagag atcattgaaa tcttagaatt
     3601 atcgaaagaa gatgcattgc gcaaattatt gatcgaaaca cttgaagcac aagtgcgtgc
     3661 attaaaaact tatcagtctg aaagcggttt atggcatacg ttgttggatg atccttcttc
     3721 ttacgtagaa tcttctgcaa ctgcaggatt tgcttacggt attttgaaag cggtccataa
     3781 acgttattta ccacaagaat ataaagaagt cgcttatcgt gctatccaag gattgcttga
     3841 acaaatcgat gaaaaaggag aagttcaaaa cgtttcaatc gggacaggaa tgggggacag
     3901 tttagatttt tatcggaata tcggaatcac agcaatgcct tatggacagt cgttaacggt
     3961 gctttgtttg acagaattgc tcgtctctta ttgttaaaag aaaggaaacc catatgaaaa
     4021 aaacatcact gctgtttgca gcaagttcgc ttagcctagg tctgctaggt ctaagcggct
     4081 gcggaaataa ttctagtgtg gcaactgtcg cagatgcaga cgtaaatgag atagttacct
     4141 tgcgctttgc ttatgcaagc aacagccagt aattgatgca atgaatgaat ttgggcgttt
     4201 agtaaaagaa aaaacagatg gttccgtaca gatccagtat ttcccagatg gacaactggg
     4261 tggagaacgt gaactgatcg aattgacaca gtcaggagca gtagatttca caaaagtaag
     4321 tgcctcagcg ttggaaagtt tctctaaaga ttattcgatt ttttccgttc cttatctatt
     4381 caatgatgaa gaacatttct ttaaggtgat ggataatgaa gaaataatgg agccaatcta
     4441 ccaatcgacc aaaaatttag gatttaccgg attgacctat tatgattctg gtcagcgcag
     4501 cttttatatg gtagacggtc cgatccatac gccggaagat ttgaagggaa agaagatccg
     4561 tgtgatgcaa agcgaaaccg ctatcaaaat ggtagagctt ttaggcggtt cgcccgtacc
     4621 tatgggaagt gatgaagtgt acacgtctct tcaatccaat ttgatcaacg gatcagaaaa
     4681 caatgaattt gtcttgtata cagctggaca tggcggggta gctaaatact attcctatga
     4741 tgagcataca cgtgtgccag atatcatcat catgaatgat gccatcaaag aacagctaac
     4801 tgatgaacag gaaaaagcca ttgaagaagc agcgaaagaa tcaacggttt ttgaaattga
     4861 agcttttgca aaagcaattg aagaagaaaa agctttagca caagaagaac atggagtcca
     4921 attcaatgat gtagataata caccgtttcg agaggcagtt gccccacttc atgatatatt
     4981 caaaaacgat cctacctaca aagaccttta tcaaaagatc caagagcaag gagcgtagct
     5041 gtaatgaaaa tgattttaaa caaggtatta gagatcctgg gagctgtttt gcttgttggg
     5101 atggtcgctg ttgttttatg gcaagtattt tccagaacgg ttttaggaaa tccaaatacg
     5161 gtcacggaag agcttgttcg attcggtctt gtctggtttg ccatgttgtc ttccgcgtat
     5221 gttgtgggac aaaaagcacg tttagccgta acggttttaa gtgaaaaatt aactggcaaa
     5281 aaaagtaaaa ttttagaagt tgttgttcaa agtttatttc tggtatttgc tgcgatgatc
     5341 atggtgtatg gcggatggaa tgctgtcaca ttgacaatgg ggcagatctc gccttcttta
     5401 agtattccta tgggatatgt ttatctgtca gtacctgtat cgggggtact catcattatc
     5461 tatagcctga tcaatctaat cgatacatta agcaacaagc cagcaattca agaaaaaata
     5521 taaaaatgga ggaataaaat ggcaatagaa gccggattag tgttattttt tgtatttgtt
     5581 gtgttgcttg tattagggat gccgattgct ataagtattg cattctcatc gatgatgact
     5641 ttattgttag ttataccgtt tgatgtttct gcttttagtt cagctcaaaa aatggtggga
     5701 agtatcaata gtttttcgct gatcgctatt ccgtttttcg ttctctcggg cattatcatg
     5761 aataatggag ggatagctaa gaaactagtg gattttgcga tgttgttcgt cgggcgcgtg
     5821 ccgggggctt tggctcatac gaatgtgtta gggaatgctt tatttggttc catctctagc
     5881 tcggccattg ccgcttctac cgcgattggt ggagtgttga tccctcagca agtggaagaa
     5941 ggatatgacc ggaagtttgc gacagcggtc aatatcgcgt cagctccgac aggtatggtc
     6001 attcctccaa gtactgcttt tatcatgtat tcacttgttg ctggtggtgc ttcgatctct
     6061 gctttgttta tgggaggtta tctcgtaggc gctt
//
LOCUS       DAZKNO010000147         6009 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000147 DAZKNO010000000
VERSION     DAZKNO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.150"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..487)
                     /gene="yajC"
                     /locus_tag="ACG8MY_002236"
     CDS             complement(107..487)
                     /gene="yajC"
                     /locus_tag="ACG8MY_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0431403.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(584..1729)
                     /gene="tgt"
                     /locus_tag="ACG8MY_002237"
     CDS             complement(584..1729)
                     /gene="tgt"
                     /locus_tag="ACG8MY_002237"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0431404.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1947..2645
                     /locus_tag="ACG8MY_002238"
     CDS             1947..2645
                     /locus_tag="ACG8MY_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0431405.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2813..5452)
                     /locus_tag="ACG8MY_002239"
     CDS             complement(2813..5452)
                     /locus_tag="ACG8MY_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0431406.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
BASE COUNT         1689 a         1318 c         1104 g         1898 t
ORIGIN      
        1 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
       61 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
      121 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
      181 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
      241 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
      301 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
      361 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
      421 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
      481 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
      541 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
      601 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
      661 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
      721 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
      781 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
      841 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
      901 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
      961 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     1021 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
     1081 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
     1141 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
     1201 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
     1261 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
     1321 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
     1381 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
     1441 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
     1501 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
     1561 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
     1621 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
     1681 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
     1741 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
     1801 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
     1861 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
     1921 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
     1981 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
     2041 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
     2101 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
     2161 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
     2221 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
     2281 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
     2341 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
     2401 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
     2461 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
     2521 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
     2581 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
     2641 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
     2701 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
     2761 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
     2821 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
     2881 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
     2941 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
     3001 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
     3061 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
     3121 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
     3181 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
     3241 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct
     3301 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
     3361 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
     3421 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
     3481 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
     3541 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
     3601 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
     3661 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
     3721 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
     3781 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
     3841 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
     3901 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
     3961 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
     4021 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
     4081 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
     4141 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
     4201 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
     4261 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
     4321 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
     4381 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
     4441 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
     4501 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
     4561 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
     4621 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
     4681 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
     4741 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
     4801 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
     4861 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
     4921 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
     4981 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
     5041 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
     5101 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
     5161 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
     5221 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
     5281 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
     5341 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
     5401 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
     5461 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
     5521 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
     5581 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
     5641 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
     5701 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
     5761 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
     5821 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
     5881 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
     5941 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
     6001 cggcttttt
//
LOCUS       DAZKNO010000148         5979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000148 DAZKNO010000000
VERSION     DAZKNO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.151"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG8MY_002240"
     CDS             352..1041
                     /locus_tag="ACG8MY_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0431407.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG8MY_002241"
     CDS             1028..2236
                     /locus_tag="ACG8MY_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0431408.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG8MY_002242"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG8MY_002242"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0431409.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8MY_002243"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG8MY_002243"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0431410.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT         1831 a         1129 c         1160 g         1859 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgt
//
LOCUS       DAZKNO010000149         5954 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000149 DAZKNO010000000
VERSION     DAZKNO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.152"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(797..1015)
                     /locus_tag="ACG8MY_002244"
     CDS             complement(797..1015)
                     /locus_tag="ACG8MY_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431411.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(1030..3603)
                     /locus_tag="ACG8MY_002245"
     CDS             complement(1030..3603)
                     /locus_tag="ACG8MY_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0431412.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTRNVVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVMNTIVVGLMSLSLMFVGYKMITGKGTIDLKSVGTNIVMSVALI
                     LLMPTMISSGIQFSKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVE
                     DRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDNLLK
                     K"
     gene            complement(3590..3808)
                     /locus_tag="ACG8MY_002246"
     CDS             complement(3590..3808)
                     /locus_tag="ACG8MY_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431413.1"
                     /translation="MIEKRLIYQIYCLDHNADTLELIESICFNTIVLDLKAKNDCYVT
                     HIIIDRKTAHKLSESLRKWAEGENYESN"
     gene            complement(3823..4698)
                     /locus_tag="ACG8MY_002247"
     CDS             complement(3823..4698)
                     /locus_tag="ACG8MY_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HFQ0431414.1"
                     /translation="MAEKRMLAKSFLNNASFMKMSDSAKLLYVYLNTNADDDGIVEAY
                     PYMKMLGTPDDDVKILFTKGFVFFLNEDLLTFLPHWLQNQNIRIDRYKPSIYRFKLLN
                     LKPEFFSKLKPVKLDVILKSGQVEPLRGDLMEKWYPAGIPSDVKSDTQLSQGQDSQDQ
                     TNQDKDSIEEINQDQNSFFENIINQKGIEIITTLGDRYTEVQREKYLKLIASMKDSIE
                     REYKIEIRGQDYFDITNKMLTRLFNSINEKLNLGEKIDEMKFINTTARNHWEDIAKQE
                     KASRQDMLEALKKIG"
     gene            complement(4767..4928)
                     /locus_tag="ACG8MY_002248"
     CDS             complement(4767..4928)
                     /locus_tag="ACG8MY_002248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431415.1"
                     /translation="MNDDEVAKLILKEIEMYLQFDILQREYAEKGIKNALKKIKRSDP
                     DSLQTKGST"
     gene            complement(4973..5242)
                     /locus_tag="ACG8MY_002249"
     CDS             complement(4973..5242)
                     /locus_tag="ACG8MY_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431416.1"
                     /translation="MEVILPPDLDRELRNYFYHVAREAVDQVRIETSFNKEVFRLGEA
                     CEYLSVSRSTLKKFIREKGLKVSVINDCQFIRKEELARFIKEHEV"
     gene            complement(5259..5459)
                     /locus_tag="ACG8MY_002250"
     CDS             complement(5259..5459)
                     /locus_tag="ACG8MY_002250"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0431417.1"
                     /translation="MLAEIIKIKRIERGLTQEELARIVGISRSYYSDIENNRTIPSGK
                     VLLNLNKTLKFFKINDVNNGIK"
     gene            5594..>5954
                     /locus_tag="ACG8MY_002251"
     CDS             5594..>5954
                     /locus_tag="ACG8MY_002251"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431418.1"
                     /translation="MIDIGNNIKNIRKSKKMTQTDFAELMGLSRSYIGDLENNRSNPS
                     IKTLEAIARALNVDLQKIISSDRTLNAKDNTLKIIEYTQLYNNKYLFKLAGTVTHKWN
                     FIYANVDLSAINLKNKYY"
BASE COUNT         1730 a         1157 c          773 g         2294 t
ORIGIN      
        1 cttttctgtt cacaaaatgg gtttaaattt gctttcttta acacttcatt tatatcatag
       61 caattataat gtttacacaa catcattcct aattttaacg gttgttcctc tttatatttt
      121 ttaagttcta catttttttg cgtttaatta cttttctctt ttctttatat tccgctccaa
      181 ctttctcttt aatagtgaca atagaattac atatttttgt taaacttcct taaaaaaatt
      241 ggatctcgat tactcaaatt tgcttgcagg cttttctaat tgctttgttt tttcttccta
      301 attatttgat tttatttcca ttttcttcta gtgaaaattt attattttta ataatcgtac
      361 gcgtagaact gatttcattt tttgtatctg tttgattatc taacgtttga aattcaaagt
      421 tttgattggc tttatcaata tctttttata ctactttatt ttatttgcaa gatatatttt
      481 cttgcttaat ttggtcatag tttcattcgt cacttcggtg atattttttt aatttgcaaa
      541 taaaaagagt tgaaccgata tacattaggt tcaactcttt tgcaggtttc atgggtgtac
      601 tatcactttt aatctgatag atgtaaacct ctctttatct ttttgttact tcaattttca
      661 acaatagctc ctttataggt tgtcaatatt gctctacgta ttctggcttt ttaacccatt
      721 atttctctaa agtggaagag aataatcgct taatccccac taattccttg tcgtaaattc
      781 atgtcaaata caatttttaa aaaccattta attcatggat tatctcttca cttttttcta
      841 gataacaata tttcaagttg ttttttatct catgtttcat ctttaattct tcttgaatca
      901 tcagttcaat ataattagtg accgtgattt tttcatttgt tgcgttcttt actgattcac
      961 ataattcgtc aagttctaag aaatcatgcg tactaatctt tagaattaat ttcatgacaa
     1021 ttcccccttt tattttttta atagattatc gaaaagcgga ttaggatcat taagtgtatc
     1081 tctgaataac tcgttgtctt cataagtgaa tcttcgtctt ggttttgatc ccttatcatt
     1141 ttcacggatt tcactatcaa taatcactgt ttcatgattt gaatcaaatt ttttcgctga
     1201 ctttctgtct tcaacgatat tgattttttg tgttttttct ttcatttctc cactatcttt
     1261 ttgaacgttc tcccgaacat ctactgtttt gaatccttga ctacttgtag aactttgtac
     1321 attctctctt acatccattg atttaaaccc ttgactactt gtagaatttt gtacattttc
     1381 tcgaacatca attgtcttag taccttgacc atttgaagag ctttgtaaat tctctcttac
     1441 atcaacattc cgtccgacat gaccattcaa tgaaccttga atattttctc ggacatctac
     1501 tgatcggcca ccttgactac ttgtagaatt ttgtagattt tctttgacca atttttctaa
     1561 tccggtattt gagtttttag cttcttggat tctttctttg gtcaacgctt ctttttcgtc
     1621 cgtattagct cctgtacgcc cttctacacg tttccttaac atttcctcac gattcattgc
     1681 ttcaggcgtg tatttagctt cttgtcgctt ctgattaatt aattcttcag cagacttagc
     1741 ttgaccgtgc atagcatttt tacgttcttc aatatcttgt tgaacttttt gctgaattgc
     1801 acttacagga ttattattcg attcagcatt atttttcatt gcttcacgca tacgttcaga
     1861 actagacata attctatcgg cattttcaac cttagcacca tcttttctat ccgaaagacg
     1921 tgttggcatg gaatcagcat attttccagt ttcatcttct ttatttttag ctaacatagg
     1981 tttgctattt ttattaatag cggacactgt accatcatct aaaccttctt tgaacttatt
     2041 cttagtatca ttagccatat cacgcatact cttaaatggt tttattgctc tttcactagc
     2101 cattgtagct ccatctgaag ctaaacctga taatccacgt tcatgggcat atcccaaagc
     2161 tcttccagtt ttgccagctt tttttgatag agtttcgccg aaattctttt ctggttttcg
     2221 atcttctcct tctccactgt ctgatccatt tccatttccg cttcctttag cacgattaaa
     2281 tccagttgat ccttttctaa acagcattgc gcccatacca aaagcagaag caagttgccc
     2341 atagccttct ttgagtccaa tatcaacacc aaagtatctc atgactgttc cactgccttt
     2401 tatcagcaca aacacagcac agatataagc aattgttttt ataaaaatat ttgtggagat
     2461 accttgtcca gcatttaaaa aattgataaa catagcaaac atagatagtc caaacccttg
     2521 aaatccaact gttaagtaac attgtaaaat atcggataaa acaacttttg aacgttgtcc
     2581 tgtttcaata tctgtcgcaa aaacaagaac acctaacaca cgtttaaatg caagttctaa
     2641 tattgctgta atgatcacaa acgcgctgaa cacataggcg attgctaacg ctgtcaatcc
     2701 aatcgaaatc ccccaaagat ttgcttgata gcgataatag cctgatttca aactatcaga
     2761 aaatgttgat aagaagttat cagagaactt tgtagcaaca aattcattat tagaattttc
     2821 aactaattca tagcgaagat tatccgcagc tggattttct ttttccaatt tatcaatcac
     2881 tttatccgtt aacacttgtg tgagatccgt ctgttgaaaa ttttttcgtg ttaacttatt
     2941 tcgatcctcc gttttatcaa tgcttgagta ctgatctgtt ttattaatat atgccaaatc
     3001 tgttactcct tgttttatca atgaccaagc gactccgtca tctgaatttg taatcgtagt
     3061 agcatcattg taaaatattt tggaaaattg aataccgcta gaaatcatcg ttggcattaa
     3121 aagaattaat gctacagaca tcactatatt cgttccaaca gattttaaat ctattgttcc
     3181 ttttccagta atcatcttgt agccaacaaa catcaatgaa aggctcatta atccaacgac
     3241 aattgtattc atgactgatt gataaacgtt atttagccct gtactttcaa tgaaattgaa
     3301 caaatccagc acatctggaa tcatattttg aatcgaatca gtcacagtaa acatgccttt
     3361 taccacagcc catccaagcc aaatcccgaa ctgactaagg aaatctccat tgtatttttt
     3421 caggtaaggt ctataaagag aattattcaa aaactctgtt tgactttttg tgtcatcaaa
     3481 ccaattgaaa atgctagctt caacaacatt ccttgtaaaa acaacgctaa tcattaaaac
     3541 aagcacactg agtgctaacc agtttatctt tctactaacg attttttctt taattgcttt
     3601 catagttttc tccttcagcc cattttctta ggctctcaga gagcttatga gccgtttttc
     3661 tgtcaattat gatgtgagtg acgtaacaat cgtttttagc ctttaaatcg aggactatag
     3721 tattgaaaca gatactctcg attaactcaa gagtatctgc gttatgatca agacaataaa
     3781 tttgatatat taaccgtttt tcaatcatat tattaatcct ttctaaccta tttttttcag
     3841 tgcctctagc atatcctgtc tactagcttt ttcttgctta gctatgtcct cccaatggtt
     3901 tctagctgtg gtgttgatga acttcatttc atcaattttt tcaccgagat tcagtttttc
     3961 gtttatagaa ttaaaaagtc ttgttagcat tttgtttgtt atatcaaaat aatcttgacc
     4021 tcgaatttct attttatatt ctctttcaat tgaatctttc atactagcaa tcaacttaag
     4081 atacttttct ctttgaactt ctgtatatct atcacctagt gtagtgatta tttcaattcc
     4141 tttctgattt ataatatttt caaaaaagga attctgatct tgattaatct cttctatact
     4201 atccttatct tgattagttt gatcttgact atcctgacct tgacttaact gtgtatccga
     4261 tttgacatcg cttggtatac cagctggata ccatttttcc attaaatcgc ctcttagcgg
     4321 ttctacttgg ccagatttca aaatcacatc aagttttact ggctttaatt ttgagaaaaa
     4381 ttctggtttt aagttcaata acttgaagcg gtaaatagat ggtttatacc tatctattcg
     4441 tatattttgg ttttgtaacc aatgggggag aaatgttaac aaatcttcat ttaaaaaaaa
     4501 tacaaaacct tttgtgaaaa gtatctttac atcatcatcg ggtgttccga gcattttcat
     4561 gtatggatac gcttcgacaa ttccgtcatc gtctgcgttt gtgttcagat atacgtataa
     4621 taattttgct gaatctgaca tcttcataaa acttgcatta tttaaaaatg atttagccag
     4681 cattcgtttt tctgccataa aattcacact tcttcctatt tgaatgttaa aatgtagtca
     4741 aataaagttt tctcagaact ttattgttaa gtgctcccct ttgtctgcaa actatcagga
     4801 tcgctccttt ttattttctt caaagcgttc ttaatacctt tttctgcata ttcccgttgc
     4861 agaatatcaa actgcaagta catctcgatt tcttttagaa tcaactttgc tacttcatca
     4921 tcgttcaaac aaacacttcc tttttcatat caaaagcacg ccacgcaact ttttatactt
     4981 catgttcttt gataaacctt gctaattctt cttttctaat aaattgacag tcattgataa
     5041 cactaacttt taatcctttt tcacgtataa atttctttaa tgtacttctg ctcacactta
     5101 aatactcaca cgcttcgccc aaccggaaga cctctttatt aaaagaagtt tcaattctaa
     5161 cttgatctac cgcctctcta gcgacgtgat aaaagtaatt tctcaattct ctatccaaat
     5221 caggtggtaa aataacttcc aatattactc ctccttactt atttaattcc gttatttacg
     5281 tcatttatct taaaaaattt tagggtctta tttaagttca ataaaacctt ccctgacgga
     5341 atagtacgat tattttctat atcagaataa taacttctcg atataccgac aattctagct
     5401 agctcttctt gcgtaagccc tcgttcaata cgctttatct ttataatttc agctaacatt
     5461 acaatccccc ttccaatgac gtacatttac gtctaaaaat atcatactgc actgcagttc
     5521 ctaagtcaac aaaaatgttg tgtatttacg tcatttaaca tatactactt tatatagtgt
     5581 ggaggtgaaa tgtttgattg atattggaaa caacatcaaa aatattagaa aaagtaaaaa
     5641 aatgacacaa actgattttg ctgagttaat gggtctatca cgatcatata taggggattt
     5701 agaaaacaac aggagtaatc ccagtataaa aacactggaa gccattgcaa gagcattgaa
     5761 tgtcgaccta caaaaaatta ttagttctga tcgaactcta aatgccaaag ataataccct
     5821 aaaaataatc gagtatacac aattatataa taataaatat ttatttaaat tggctggtac
     5881 ggtgactcat aaatggaatt ttatatatgc aaatgttgat ctatcagcaa ttaatctcaa
     5941 aaataagtac tata
//
LOCUS       DAZKNO010000150         5949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000150 DAZKNO010000000
VERSION     DAZKNO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.153"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..974
                     /locus_tag="ACG8MY_002252"
     CDS             168..974
                     /locus_tag="ACG8MY_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0431419.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            986..2206
                     /locus_tag="ACG8MY_002253"
     CDS             986..2206
                     /locus_tag="ACG8MY_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431420.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2196..3782
                     /locus_tag="ACG8MY_002254"
     CDS             2196..3782
                     /locus_tag="ACG8MY_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0431421.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3821..>5949
                     /locus_tag="ACG8MY_002255"
     CDS             3821..>5949
                     /locus_tag="ACG8MY_002255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0431422.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSVIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSL"
BASE COUNT         2017 a          964 c         1247 g         1721 t
ORIGIN      
        1 cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat
       61 cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta
      121 aaatggcact tgactgtaat tcactgaaat cgcttggagg aacacaaatg tttaaagatt
      181 tgtatttatt tatgaaagac atttatcaga atagaaaatt actgctacag ttttcgatca
      241 atgacttcaa atcaagatat gcaggatcgt ttttaggaat cttatgggcg tttatcaatc
      301 cgctatttac tgtattgatt tattggctgg tatttggtgt tggactgaaa gcacaaatga
      361 tcgatgcaaa atacccattt atcgtatatt tagttaccgg gatgattccc tggttcttct
      421 tctcagatgc atttgcatca actacggttg tttttcggga atatacttat ttagtaaaga
      481 aagtcgtatt caatattcgt atcttgccca cgacgaaaat cctatccaat ctgtacaccc
      541 atttattctt cattctgatt ggcatagctg tcgcgcttat aaatggtata tatccttcag
      601 caatgtcttt acagctgatt tattatttat tttgcatgtg tgctttcttg acaggattga
      661 cttggctgac ggcatcgatc caaccatttt tacctgatat catgcaatta attacgatcg
      721 tgctgcagtt gatcatgtgg acattaccga tattatggag ccctaatcag tttgattcag
      781 gtcttgtaag catattgaaa ttaaatccgc tgtactatgt tgttcaaggt tatcgtgagt
      841 catttttaag tgaagcttgg ttctgggagc atggaacata tactttgtat ttctgggttt
      901 tcacgttagt aatgttcgta atcggctctg cagtgttccg tagattgaaa ccacattttt
      961 cagatgtgct gtaaggagaa tagctatgac agaatatgca attagactaa aagatatcac
     1021 aaaatcttat aatatgtacg caaaaccttc ggatcgattt cgtgaagccc tcaatccttt
     1081 taagaaatcc tatcatgatg tattttatgc gttgaaaaac gtgaatgtag aaataaaaaa
     1141 aggtgagatg atcggtttcg tgggagaaaa cggatcggga aaatctacaa tgttgaaaat
     1201 cattacggga gtactgacac caacatccgg cacgatggaa atcgaaggaa aaatctcagc
     1261 tttgctagaa ctaggatcag gattcaatcc tgagtattct ggctatgaaa atatctattt
     1321 aaatggaatg gttctaggat tttcaagaga agaagtcgat gaaatggtcg atgatatcat
     1381 tgaatttgct gatattggcg accatattta tcaacctgta aaaacctatt caagtgggat
     1441 gttcgttcgt ctagcttttg ctgtagcgat caatgtgaat cctgatattt tgattgtgga
     1501 tgaagcattg gcagtaggag atcttgaatt ccaactgaaa tgtatggaaa aatttacaga
     1561 aattaagaac tcgggaaaaa cgattttatt cgtttctcat gatattagtt cgattcgccg
     1621 tttttgtgat cgttcctatt ggttgaaaaa tggagaagtt gtcgaatatg gtgatacgat
     1681 ggaagtaacg acgaattacg aaaacttttt gaagaaaaaa tcagttaaaa cagtagatcg
     1741 aaataaaaac caagtagaac gcttcgcagc tccagatatc gtagacgtga cgaaagccga
     1801 attgttggat aaaaataaaa atccagtgga tatgctggaa caaggagaaa aactatacgt
     1861 taaagtaacc tatgaagtaa aagacgatac aatcaaaaat ccagttttag gaattgctct
     1921 gcgtacagtc gacaattttt atgtgtgcgg actcaatact ctactagatg gtatcaagat
     1981 cccttggaaa aaaggtgaga atatctttta tctggagtat gaaaagttag gtttgttagc
     2041 tggtgaatat tattttgacg ttgcggtctt tgaagaaaat gcaacggttc ctttggtata
     2101 caagacaaaa tacatgaact tgtttgtcag cggttcttat atcggagaag gcatcgtggt
     2161 cttagaccat aaatgggaag aagggactca tagcaatgaa atatgatttt gaaatggacc
     2221 tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat caaaccagga agtaaagttt
     2281 tagagtttgg tcccggaaac ggtcgattaa cgaaacattt gatcggagca aaacaatgtg
     2341 aagtgagcat tgttgagcta gataaggaat tattcgattt tgtcagcgaa tttgcccaag
     2401 atggatttta tggagatatc gaaagttttg aatgggcaaa ctattatgct ggacagacat
     2461 ttgattacgt cttgtttgcg gatgttttag aacatttagt agatcctgga aaaacattga
     2521 agaaagtcag agaatttttg aatgaagaag gagagatcct gattacattc cctaacttag
     2581 cacataattc tgtcatgatc gatttgttca ataatcaatt gccttgggct tcttatggtc
     2641 tcctagatga aacccataat tctttttata ctcatgatgg tttccaaaaa gtttttgaaa
     2701 aagccggatt attcattaat attgaagatt acttgtactt ggctgtagga gatacagaat
     2761 taaaatcaac ttatgaagag ttgccggaag ctgtacgcta tgatttcaaa atgcgaccat
     2821 ttggtgaagt ctaccaatac tttttctcat tgatgaaaca tccagtagta caaacttcca
     2881 ttgcacagcc acaaaactct aattatgtaa aagtactaga agtaacccag caaacgaaac
     2941 aagatgaaac aatccaacaa ataccattca ataatttcac aggggaaaat gagacactaa
     3001 gtttccccgt gtcagaagca acagaaagaa tggtgtttcg cttcgcacag caacctagct
     3061 ttatcgaatt cagtgcagaa gctgctggag aaaaactcat atttatcgat tcaaatgctg
     3121 tagtcaaaac agtaaatgat tgttatttgt ttgatgggaa agaaatacca gagttcgctc
     3181 taacggatat ttctgggaaa gaagtgacta tccattgtca ctatcgcttt atcggggaat
     3241 tgacaccaac aatgaaagaa ttgctggaaa caattcgtcc tatggctgaa gtcacaaagc
     3301 agctttctga gaaaaatgat gaattagaaa gagaaaatca gcatcttctt gaggaaaata
     3361 caagactaga ccatacgtta aaaacaacca caaatcgcta ttgcacattg ttggaaagtg
     3421 atgaatggac gatcaaacat agactaggac gacgtaagaa aaaaacatcg aaaaaaatac
     3481 aagaaaaaga gctttctata tgtattgatg agaaaatatg ggatgcagaa acaaaaatat
     3541 taaaaatcat cggatgggga atcgctaatt cggatcgcca gccattgtcc tataagttat
     3601 cagctgacca atcgccattt ttcgaagcaa ttcctgtttc acgagatgaa gtcaatcaag
     3661 cagaacagct cgcaaaagga acagaagcgg gatttgaatt acgaatcctt tgcgagcaag
     3721 aacgttcatt cttagtggaa gcagtagctc aaaatggtca atcatggatc attgaaatgt
     3781 aaataatgat taacttataa agataaggag taaacgaatc gtgcaaaaaa aagaaattgc
     3841 cgtatttatc gatcaaatca ctcgtgaacg ggccactggc gatttgctta ttgtgggctg
     3901 ggcaattgat gaagtgacca aagaaatccc tacgataaag gtagaaaaag aaaatgtgat
     3961 tgctgaagca acacatgtcg taagattgga tatcaatcat ttatataatc ttgatgttaa
     4021 gacccaaagc ggttttaaga tacgtttatc cgggaaaatg agaggaaaag cgatcttaga
     4081 ttttcaaacg gcaaaacatc aaaatggcat agctgtgaaa ctaaatggta aataccctta
     4141 tgatgacggc attgaatctt catgggaaag aaaaaaaaga ttactgaaaa aagggataaa
     4201 ttatgcaagg actcatggcg taaaaaaaac aatccgccgg gtcaaactgg aattgaaacc
     4261 aggatcgatc gactatgcag agtggatcag tcgtcacgag aaacctgatc tgaaagagca
     4321 aaggaaagaa gcacaagggt ttgcataccg tccattgatt tccatactta tgcctgtcta
     4381 taacgttgaa atcaaatggc tcgaaaaatg cattgattcg gtattggatc aaacgtatga
     4441 tcactgggaa ctatgtatca gtgatgatgc ttctactgat ccagctatcc gaaagtgtct
     4501 ggaatcctat caagcaaagg atgatagaat caaagttgtt ttccgacaag aaaatggaca
     4561 tatcagtctg gcaactaatt ctgcgttgga aatggcagaa ggagaattta tagcgctctt
     4621 agataatgat gatgaattac cgccatttgc attatatgaa gtggcaaaag tgctaaacat
     4681 acatccagaa ctggatttga tctacagtga cgaagataaa atcgatgcag atggcaatcg
     4741 ctttgatccg cacttcaaag cagactggtc tcccgataca ttgatgggaa ataattatat
     4801 ctctcactta ggcgtatatc gaacaagtat tgtgaaaaag cttggcggtt tccgtaaagg
     4861 ctacgaaggc tctcaagatt atgatttggt ccttcgtgtg acagaacaaa tccctgcaga
     4921 tcatatttac catattgacc gagtcttgta tcattggcgg acgatcccag gatcaaccgc
     4981 aagtaatggt gaagccaaaa gttatatcta tgattctgga gtgaaagcat tgacagatgc
     5041 cctgtctaga aggaatatca aaggaagcgt acgtcctggc agaatttctg gattttatga
     5101 gattgcttat gacgtcttgc aagaggattt ggtcagcgtg atcattccaa cgaagaatgg
     5161 ctatgaagat ctaaaaactt gtgtggattc ggttatcgaa aaaacgtctt atccaaatta
     5221 tgaaatcatt attgcagata atggaagtac agatcctaaa atgcaggaat tatttgcaga
     5281 atacaagcat cagctgaaag accgatttat cgttgaatta attgacattc cattcaatta
     5341 ttcacgtatc aacaatctgg cagcagaaaa agcaaacggc aaatactttt tattcttgaa
     5401 taacgataca gaagtgattg aacctgactg gatgacagcg atggtttctt atgcacagtt
     5461 tgatcgaatc ggctgtgtag gagcaaaact gttttatcca gatgatacaa cgcagcatgc
     5521 aggagtatta ttaggaatcg gtggtgttgc aggacatgca ttgaataact atgatcgaac
     5581 acattgtggt tatttcggcc gattggtcat tgacgtcaat tacttagcag tcactgcagc
     5641 ttgtatgatg gtcaaggcag ctgacttcaa tgctgtcaat ggatttgatg aaacattaga
     5701 agtagcattc aatgatgttg acttgtgttt gaaagtttat gaactgggtc gttacaatgt
     5761 ctatgcacat caagcagaat tgtatcattt cgaatctaaa tcaagaggtt atgaagatac
     5821 accagaaaaa caaagacgtt ttgccgggga aataaaaaaa atgcaagata agtggccttc
     5881 ttatattgct catgacccat tttataacga taatctaact aaagaaggaa tcggcgattt
     5941 ttcattgag
//
LOCUS       DAZKNO010000151         5882 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000151 DAZKNO010000000
VERSION     DAZKNO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.154"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            150..1046
                     /locus_tag="ACG8MY_002256"
     CDS             150..1046
                     /locus_tag="ACG8MY_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0431423.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFNMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1196..2137)
                     /locus_tag="ACG8MY_002257"
     CDS             complement(1196..2137)
                     /locus_tag="ACG8MY_002257"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0431424.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2262..3383
                     /locus_tag="ACG8MY_002258"
     CDS             2262..3383
                     /locus_tag="ACG8MY_002258"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0431425.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3641..5143
                     /gene="eat(A)"
                     /locus_tag="ACG8MY_002259"
     CDS             3641..5143
                     /gene="eat(A)"
                     /locus_tag="ACG8MY_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0431426.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5210..5737
                     /locus_tag="ACG8MY_002260"
     CDS             5210..5737
                     /locus_tag="ACG8MY_002260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0431427.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1926 a         1037 c         1231 g         1688 t
ORIGIN      
        1 ggcgaatttt aatagaagaa tgatttattt catcatattt tcatacaaat gttaaatttt
       61 catacaaata taggaagcgt tttactggct ttttactatt taaacaggct atcataggaa
      121 aaagtgacag agaaagtgag gcggaaaaga tgaacaaaaa tgacgtactg ctagttgcaa
      181 aaaataaatc actttatttt ttgccttcca tccgctcttt agatgtttgg tccggaaaag
      241 atttactaca gtcggaattc attaaatcag ctagacaaca gttggcaaat tatcagctga
      301 tcgttttttt agattatgga tttaatatgg agatggctcg acgcgtgcgc ccatttacca
      361 atgcaaaaat tgtgttattt ttttggaatc acttcaaagc agaacacgag gcgatgctga
      421 agcaggcaca agatgaaccg gcaatcgatg agatttatca ttttgatttt ttggaagcaa
      481 aagaacttgg gctgaaacat aatagttcat tttattcgaa acatatggat ttttcttcta
      541 gcgaaccaaa gacggatcta ttttttggtg caacgaataa tgggagaaaa gaacgagcag
      601 aaagctataa gcaggaattt ataaaaagag atgttacgac caactatttc attcttccac
      661 atcgagggaa tgaacaacct ggatatcttg cttatgaaga gtacctgaaa aaaacagggg
      721 aatcgcgcgg gattctggaa ctgttgcggg agggtcagca aggagtgact ttgcggacat
      781 ttgaatctat gtattttcaa aagaaactag tgacggataa tcaagcgatt gctcattatc
      841 atttttatcg tcctgaaaat atttttttgc ttcgagaacg tgatttagat gaattaccgg
      901 aatttctgca tacacctttt aagccaatcc catcagagct attggaattt tatgatgcag
      961 aaaattgggc aaaacgattt atcactaacg attccactat ttatgagcaa tacgaatatg
     1021 acctctcctc tgttgaaaat aaataaaaaa cctacatttt gaagagatta tgtaccgaaa
     1081 caaacaagca caattcttat ggcatcatga gagttgtgtt ttctttttga tctttcattg
     1141 ggcttaaaaa aagagaacca gctgctgatt cagctgattc tcatcagaga gccttttatt
     1201 ctgcgccaag gattccttct tttgcatgga ctaattgggt caaaaaggca caatatggtg
     1261 tagcaatatc atatttttgt ccttttcgcc aaacggcacc attgatgtag tcgatttcag
     1321 tcaaacgatg gtttttgatc aggtcttggt acatagatgg gtaatgcaac ccgatgttgt
     1381 caggatcata agtagaagca atatgttggt acacttcttc ttgatctaat atgattcctt
     1441 cttttgctgc gatagaagca aattcagata cgattgttcg aaccatgctc tctgccgttt
     1501 tttgcgcgcc tagttcagca atattacaat ccaaaatcgt gcataaacca ttcaatgttc
     1561 catttacaca agcttttcgc caaatggaat aacgaacatt atggctatag atcggattta
     1621 attttgcttc ttgaaagact tccacaactt tttttgtaaa tgcttcgcct tctggttcca
     1681 agttctctag ttcgatttct ccatcaccta acaatgtgac ttttccggga ccagctagac
     1741 cagctgtcca catcgtgatc ccaaataaga tatttttctt tggcacatat ttttccagta
     1801 catcttcatg acctaatcca tttagtaaac acaaaacata agtattgtca gtaataatgg
     1861 attggatcga acgaaacata tcatctaatt gattcgcttt tgttaatgct acgatcaagt
     1921 ccacgtgttc gttgctttcg atgatttctt ctggtgaata gattggcagt tttgcgacca
     1981 cttctttacc gttaaagtca gcaatcaacc catttgtacg aatggcttca atgtgtgcag
     2041 gccaacgatc gatcaagagg acctcatttc cgctttgatg tagcatcaat ccgattcggc
     2101 tgcccattgc tccggctcca gctattgcga ttttcatgtt ttttctctcc tttatcaatg
     2161 agttcccttt tagtatatag aaaaatggaa gaaaatagca ttgttttgtt tgaaaaaaat
     2221 gaaaaaaagt gtcttactta agaagaaagt gagggataca gatggatatt ttggcggcaa
     2281 ttgattcatt taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct
     2341 gggaagatcg taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg
     2401 cagaagcaat ctattacgcg ctcgggggaa catggcgcac cattgatggg atggatcttt
     2461 tattccgtca gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa aataccgcag
     2521 tcatcgaatc tgcacaagca ttggggttag atctacttga taaaccaacg gatcaaacta
     2581 tccgacttgc ttccagttat gccttaggag aacttatcaa agatgcactg cttcaagaag
     2641 tagaacaaat tattgtcact ttaggaggaa gcgggtgtag cgatggtggt ctaggattac
     2701 tccaatcctt gggtgcttct ttagaagggt acacagaagg aaatccttta ttatctacta
     2761 aaaaacttca ttttgatcga agaaacgttc ttgtttctaa agtaaaattg attatcgctg
     2821 cagatgttac caatccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa
     2881 aaggaggaat agatacgacg ctagctttct tagataaaca agcaggaaag attgctcagc
     2941 aaataaaaga tttctgggag gtggatttag atgatattcc aggaagtgga gcagcaggcg
     3001 gtattggtgg agcccttttt ttgttgggag cgaagatggt ttctggtttc gatttagtca
     3061 gccaaatcac tggattggaa aaaaagatcg aacaagcaga tgtcatcatt acaggagaag
     3121 ggcggatcga tcagcaaacg atccacggaa aagttccaca cggtgtagca gtgcttgcaa
     3181 aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccaagag ctagaaggac
     3241 tctctgattt tctcccaatt gtcttaagta ttcatttggg tccaataggt ttagaagaag
     3301 caatggaaca caggcaaaca ttagaaaaaa caaaaatctt aggacagaca cttagcagat
     3361 ttttcaccat cctcccgaag tagttgacaa aatcattttc tttatgctat cgtaatttca
     3421 aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc
     3481 attcagactg taccttgtgg agacaaggaa gcagtgtgca gattttcggg attggcttgg
     3541 ttttttcgat gtatgggtct ttttgtgtgt atccaaccct tgcatcctct ttagccagtt
     3601 ctgaaaatag gactggtctt tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa
     3661 aaatctgaca ttcggctacg acagccaagg cacattatta tttgaacaag caaatctaaa
     3721 ttttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt
     3781 actgaatatt ctacaaaaca aactacctta tcaagggcaa gtaatccatc agcaagaatt
     3841 tgcctatttc ccgcaacaga caaaagataa agaacgttta acctattacg tgttaaatga
     3901 tattacggat tttgagatat gggaaatcga aagagagctc caattgatgc aaacagatcc
     3961 tgaaatctta tggagagaat tcagcacact atcgggggga gagaagacaa aagtcctact
     4021 ggcactttta tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt
     4081 ggatatctct ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat
     4141 cgtggtcagc catgaccggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga
     4201 aaaaagtcaa ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact
     4261 tcgtgatgaa tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa
     4321 gaaaacagca gctgaaaaag ccgaatggtc tcgttcccga gaaggagata aaacaaagaa
     4381 acaagtcgga ttcatcgata ctgaatctag acgagtgaat aaaggagcag tgggtgctga
     4441 tgctgcacgg acgatgaaac gatccaaagc aatcgtgaat cggatggaga cccagatcag
     4501 cgagaaagaa aaactattaa aagatatcga atatatcgat tctttgacga tgaatagcca
     4561 agcgtctcac cataagcgac ttttaagcgt agaagatctt caattagggt atgaaaatct
     4621 gttattcgag ccaattcatt ttacaatcga gcctcatcag cgggtggcga tttcaggtcc
     4681 taacggtgca ggaaagtcat ccattatcca ttatcttctg ggggcattca acggcaaggt
     4741 tataggagaa aaatcccagc caaaacatct gagcattagt tatgtacgcc aaaattatga
     4801 agacaatcga ggaacgttgg cggaatttgc agagaaaaac caagtagact accaagcatt
     4861 tttgaacaac ctccgaaagc ttgggatgga aagagatgtt tttcataaca agatcgagca
     4921 gatgagtatg ggccaacgga aaaaagtgga attggctaaa tctttatcac agccagctga
     4981 actatataca tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga
     5041 acaactgatc ttgaacgtga aacctgccat gttactagtg gaacatgatc aaaccttcct
     5101 ggataaagta tctactgaga ttatttctct tgagagaatc taacccttgt aatctcaaga
     5161 acaacaaggt atggttaagg ggaactttgc caacgaagga gagaatcaca tgctttatgt
     5221 aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcggac gggcagatgt
     5281 gccgctgact gaaaaaggac agatgcaggc acagaaacta gcagaaaagg tagtagattt
     5341 gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag atacagcaca
     5401 agcagttgct gatcgtctca gcttgccgct gaccgttgat gaacgattgg tagaaatgga
     5461 ttttggggat tatgatggac tgccaagcaa agatgagaac tttcaaaaag cacgtttagc
     5521 gttcgctgta cgattcccaa atggggagtc agtcttagat gtgtatgcac ggatcgttcc
     5581 tttattgaaa gaatgcatcg aagacgaaga aaatgtctat ctgttagttt gccataatgc
     5641 cttgataaga gtgatcaatg cttattttca tccgatgcct aatgaggggt tctttacatt
     5701 catggtagat aatacagaat tggtttctta tgagtaatta tgtttttgtg ttatttaaat
     5761 aaaatatttc aaggcactgc ttttgcttag cagtgccttt tttaggttat gacaattctt
     5821 ttgataatat aaactcaacc tttagaaatg aaatttaatt attttaataa aagtattgaa
     5881 at
//
LOCUS       DAZKNO010000152         5687 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000152 DAZKNO010000000
VERSION     DAZKNO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.155"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..546
                     /locus_tag="ACG8MY_002261"
     CDS             55..546
                     /locus_tag="ACG8MY_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0431428.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(570..2945)
                     /locus_tag="ACG8MY_002262"
     CDS             complement(570..2945)
                     /locus_tag="ACG8MY_002262"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0431429.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            3271..3897
                     /locus_tag="ACG8MY_002263"
     CDS             3271..3897
                     /locus_tag="ACG8MY_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0431430.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            3920..4624
                     /locus_tag="ACG8MY_002264"
     CDS             3920..4624
                     /locus_tag="ACG8MY_002264"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0431431.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            4816..5649
                     /locus_tag="ACG8MY_002265"
     CDS             4816..5649
                     /locus_tag="ACG8MY_002265"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0431432.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         1697 a         1092 c         1061 g         1837 t
ORIGIN      
        1 ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt tcctatggca
       61 aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa aaatgcgatt
      121 cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac agtagaaatc
      181 aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat cgaacagatc
      241 aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa tatccatttt
      301 tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt ggtcaacgga
      361 atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa actgaaagta
      421 aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga tgatctacaa
      481 gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt cactaactat
      541 cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt tactgcttct
      601 tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg tacttctctt
      661 gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa tcgttgatct
      721 gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc agcttgcaag
      781 actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc cttctcatca
      841 taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac acctacgatc
      901 atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat cccgccaagt
      961 acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa aaatcgttcg
     1021 cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg atattgctgt
     1081 ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac gaaataattc
     1141 ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag actgtgttcc
     1201 ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt tccttctggg
     1261 aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc ctgataataa
     1321 gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc caacgactgc
     1381 tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg atacgttttt
     1441 tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa atgaagcaaa
     1501 tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc tgccagccat
     1561 tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc cggtgctttt
     1621 tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc aaattctttt
     1681 aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc ttccttactt
     1741 cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg attcaccaaa
     1801 ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc aaaaaaacgt
     1861 cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac gtcacaccag
     1921 atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc ataagtttcg
     1981 atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc ttcattaatc
     2041 cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt ctcatctaaa
     2101 aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc tgtatctttt
     2161 aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt tagataaaag
     2221 atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt agagattgtt
     2281 tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat cgttttataa
     2341 gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa tttcaaagcc
     2401 atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca ttctttataa
     2461 tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg ttcaaccttt
     2521 ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat ttcttgtaaa
     2581 tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa aagttccact
     2641 tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc tatataataa
     2701 ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta atattcatct
     2761 aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc atctttttgg
     2821 agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt acgattgtcg
     2881 atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt tgactgtttg
     2941 atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt tagcaaaaag
     3001 agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat aggatataga
     3061 atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta tgaaaactta
     3121 ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt cagataagta
     3181 aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact aatggatgaa
     3241 agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa aacaggaatg
     3301 attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat tgaccctgga
     3361 gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc ggtagcgatt
     3421 ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg cgcgtattat
     3481 caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc gattttgaat
     3541 ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc tgcagaatat
     3601 gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt ccctacccca
     3661 gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc tggcgacgcc
     3721 ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga acaattactt
     3781 catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta tccaggacat
     3841 ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa tcgttaaaac
     3901 gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac acggaaaaac
     3961 gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca caaagcaagg
     4021 acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt catttgctta
     4081 ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag aacattctaa
     4141 gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt tcgggtctct
     4201 tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga attttaaaac
     4261 atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg ctgtatacaa
     4321 cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg tatggacagg
     4381 atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt tagtcgtatc
     4441 tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc ctgttcgtgc
     4501 tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat tttctatcca
     4561 cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg actcgtttct
     4621 ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct gaacgagggc
     4681 tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt tttcttaata
     4741 ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt atcttaattg
     4801 ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct cggtattatt
     4861 gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct agcagatgaa
     4921 ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt tatccaatta
     4981 ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt ttctccaagt
     5041 aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat cgttgcttgc
     5101 ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa tttccacaag
     5161 ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat tgtcgaaaaa
     5221 cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta ccaagcagct
     5281 cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg ttctggcgca
     5341 acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga gttttcattt
     5401 ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa atatttagct
     5461 gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac attcgtatct
     5521 tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa aaacgacttt
     5581 accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta ttggtttatt
     5641 gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttct
//
LOCUS       DAZKNO010000153         5645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000153 DAZKNO010000000
VERSION     DAZKNO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.156"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACG8MY_002266"
     CDS             196..921
                     /locus_tag="ACG8MY_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431433.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACG8MY_002267"
     CDS             911..1420
                     /locus_tag="ACG8MY_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431434.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACG8MY_002268"
     CDS             complement(1490..2938)
                     /locus_tag="ACG8MY_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0431435.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACG8MY_002269"
     CDS             complement(2938..3654)
                     /locus_tag="ACG8MY_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0431436.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACG8MY_002270"
     CDS             complement(3635..3985)
                     /locus_tag="ACG8MY_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0431437.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACG8MY_002271"
     CDS             4129..4902
                     /locus_tag="ACG8MY_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0431438.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8MY_002272"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACG8MY_002272"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1637 a         1115 c          979 g         1914 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacac
//
LOCUS       DAZKNO010000154         5644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000154 DAZKNO010000000
VERSION     DAZKNO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.157"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..1830)
                     /locus_tag="ACG8MY_002273"
     CDS             complement(55..1830)
                     /locus_tag="ACG8MY_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0431439.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
     gene            complement(2041..2943)
                     /locus_tag="ACG8MY_002274"
     CDS             complement(2041..2943)
                     /locus_tag="ACG8MY_002274"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0431440.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3222..5567
                     /locus_tag="ACG8MY_002275"
     CDS             3222..5567
                     /locus_tag="ACG8MY_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0431441.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT         1788 a         1120 c         1081 g         1655 t
ORIGIN      
        1 caccgtttat tcggaaaaag gaactgcgac aagacttttg tcacagttcc ctttttattt
       61 ggaagataaa gcttttttca gattcaattt actaaaaact agcatgcttg ttactgcgat
      121 gaattgcgtt aatggcacgc ctgcgccagt gtagacactt aaaaaataat cctttttttc
      181 tggcgcataa gaacaaaacc acgagacacc gaaaagaaaa accaattgtg tcagtattaa
      241 tccgatgact acaatcaaag aggaactata ataggcacga tcagcttttg caaatacggt
      301 tcctagtatc ataagtaata tccctgctat tgaagcaatc agcacgggaa tggccagaac
      361 ttggtaaatt tttatcatga cattcgcgac ttttgctgtt tttgtcaact ggaaatcagg
      421 tgttctcgga tcactgctat cgatccaatt atccattagt ggaagattca ataattcttg
      481 tgcctgttcg acttgttctt gcgtcccaaa actataattc cccccttgat ggaaatcttt
      541 gtatcgccat gcataagcat atcctgattt cagaaaatca aatacgattg gcagatcatt
      601 catcgtcttt ccatcccctg atcccatcag atagaattct tttttctttt tgatagttcc
      661 cttttgatag gcttcttcta attcttgatt gacttttttc caaaaatcat tggtttcttt
      721 tgcattgttt tggtaataac cagattcgct taccacatct cgcaatgccc aaaaaatgat
      781 atctcctggt aattctttta cttttccgcc ttgggtccac gcatgagtcg tatacaattc
      841 gtcaatacga gtagaaacag tatgaaatgt aggagatgcg tctaaagctt tatttaatgc
      901 agcatgcgag acccatatct cactgttttc tttatttaag tttgtttcgt catctattcg
      961 gatcagctgt ttcatcaaac gtccaaattc gccactcgaa cggtcattga tggctgcaat
     1021 accatatgtt gtctcgtttt tatattcgat ccactgatag acgatggaga aaccgactag
     1081 agggagtaaa aacaatgaaa gtttcagcca ttcttgtttc gaaaggagaa atggtcgttc
     1141 ttttaacttc tttgcttctt tgaaagaaaa gtcttgtcct agcaagatct gaacgaaact
     1201 gatgcctaac gcgactacag taaaaggaag aagccagatg gaatcttcgc gaatgaacca
     1261 aaagaaagga aaagaaacag ctagccctaa aaaccacggc agcaattttt tgctggaatt
     1321 atatttgcgg taataaatag ctagtaaaca agcgataatg atctcaacag tcgggatcac
     1381 gagagcatta cgatatgttc gagtggaata ttcatgtgta aaactaatcg gagaaaacaa
     1441 aaaataaaaa tagatactta aaagaagata cttgttcttc acgattggtt taaacgctag
     1501 tgcaaccaat atactggcaa gaatactgaa catgccgaat aaaactgcat aagaaagatg
     1561 cagtttatgt gcaagtacta aaaacaagct gtaggaaatc ccttttgaaa gtgtcgtagt
     1621 cgtgtactcc cctagccatt taccatccaa aatattttga gcatagtgaa tcagcagctg
     1681 atcatcatag gtcgccgaca tatctgttcc atagattgct ttactatata aatacaaccg
     1741 aataattgta aataatacgg ctatgccaag aataaagtat gtatgtttct tttttagttg
     1801 gtcgatcaag gggtttaaaa tgttcagcat cttttcgtcc tttcaatcac gttttgctac
     1861 ttgtttgtga aacttttcgt tttttacttt tctatcagta taccctgttt gtctgaattt
     1921 aaaaagaggg ggcaaaagga gcatggcgaa aacgaatgca aaaagcactc ctttgtttct
     1981 taaacaaaaa aggacaaggc agactgccca atcctttttt gttgctgtca ggtagaaaac
     2041 ttaaagtgtg acttcttctg ctaataactc agggaacact tcgggatcga atgcttgcgc
     2101 tttgaacatg gaaatttctt ttccatatgg tgcgtaagct ttctttttat ctttaccgac
     2161 atatggggtt tctaatattt tcgaaatgtt gtcaaacatt ggatcatgga cgattttgtt
     2221 caatgtgtca aatccaattg ttccaaaacc gatattggca tgccgatctt tatgagaacc
     2281 ttgaggattt ttagaatcat tgatatgcag tactttaagc cgatctaatc cgatgacctt
     2341 atcaaactct tccattacac ctgaaaaatc ttcttttata ttatatccgg catcattcgt
     2401 atgacatgta tcaaaagtaa cggataattt ttcattcaat tttactccat cgatgatttg
     2461 cgctaattcc tcaaatgttc gaccgatttc cgtacctttc cctgccatcg tctccaaagc
     2521 gatctgtgcc gtctgttctt ttgtcaaaac ttcattcaac ccctcaataa ttctagcaat
     2581 cccagcttct gcccctgctc ctacatgagc gcctgggtga agggtgattt gagtagcacc
     2641 taaagcttcc gcacggacaa tctcttctct cagaaattgt acagcaaatg gaaacatttc
     2701 tggtttgatc gtatttccca aattaatgat ataaggcgca tgaacgacga tatttgttag
     2761 tccgttttct gccataaatg cttttccggc atcaatattc atctcttcga tagcttttct
     2821 tctagtattt tgaggagcgc ctgtatagat cataaaggtc gaagatccgt aactggctgc
     2881 ttcttctgcc gcgcctaaca gcattttctt tccgctcatg ctgacgtgtg agcctaataa
     2941 catggatgga ttcctccttg aaaggcaatt gtttcgaaca attcctgtat attgataaga
     3001 tgatgtacaa agaaaatagt ttgtttttct ttgccctgtt ctccatagta actgaaactt
     3061 tccctaaaga aaagcttaag aactctttca taaaaaagat catttcctca cgttttatct
     3121 cattcgaaaa agaaattcct gtcaaaaaca gccttgtctg tttttttgtc ctattatttg
     3181 ctataattag cgaaataaaa ccattagttg aggtgaccct tttgccacgc aaaaatactc
     3241 gaaaaaaaca gaatagaaga aaaaaagaaa aatggtttgt tcctaagatt atttttagag
     3301 tgtttcaatc attgactgtt tttattactg ttcttctttt gttgttcgca gctctaggta
     3361 tcggaatagg tgctggctac tttgcctatc ttgtcgaaga cacacaattg ccgactaaaa
     3421 aagaactgca gacagaacta ggaaacatta cggaaacgtc aaagattgtt tatgcagata
     3481 atacagagat atcaaaaatc cagacagatc tgatgcggac gactatttca tctgataaga
     3541 tttcgccttt attgaaaacc gcgattatca gtacagaaga tgaatacttc gataaacacc
     3601 aaggttatgt ccctaaagct gttttacgag cactagtttc agaagcgact ggcatcggct
     3661 cgtctggagg atccactttg acacagcaat tagtcaaaca acagatccta accgatgaaa
     3721 caacattcaa acgtaaagca aacgagatcg ttctttctgc acaagtagaa aaacactttt
     3781 caaaagatga gatcattact acgtatttga atgtctcacc atttggtcgg aataataagg
     3841 ggcaaaatat tgctggagtc caggaagctg ctagaggaat ctttggtgta gatgcaaagg
     3901 atgtgactct gccgcaagct gcttatatcg ctggtttgcc tcaaagccct atcacgtata
     3961 gtccatacac gaatacaggc gcgctaaaag aagatctttc tgccggtctt gccagaaaag
     4021 attttgtttt attcagtatg taccgtgaag gacaaattac gaaagaacaa tacgaagaag
     4081 cgaaagccta tgatcttaca aaagactttc tcccacaaca aatcgcggaa cagactgaca
     4141 gagaattctt gtattatacc gtgatgaacg aagcaacaag gattattgca caacaattgg
     4201 cagaaaaaga taatgctgat atgtcagatt catcggtcag tgaagcttat tatcaaaaag
     4261 cacagcaaac gatccaaaac aaaggatata cgatccattc gacgattgat aaagatatct
     4321 atgctgcatt gcaaaccggc gtagaaaatt atggctacat gttagatgat ggtgctggta
     4381 gccaagtgga gactggaaat gtattgatgg ataacagaac cggacgtatc tatggcttcg
     4441 tgggtggtcg gaattacagc cagaatcaaa ataaccacgc ctttgataca gaacgtcaag
     4501 ctggttcatc catcaaaccg gttcttgttt acggtcctgc aatcgatatg ggacttgtcg
     4561 gatcagaatc ccgtgtatca gattatgcaa cgacttggca agaaggaaaa aatgctggtg
     4621 agaaaatcgt caatgcgacc aatgaagggt cgaacacctt ccaaaccgtc cgtgagtcac
     4681 tggaatggtc caacaatatc ccagcttatc acttatatca agatgtgttg aattccggtg
     4741 gatcgaaaca atacgcttac gagcactact tggcgaagat gaactaccca gcaaatgata
     4801 attggggcgt tgaatcggct cctttaggga cagtagatgt aacgacgctt caacagacaa
     4861 atggattcca ggccttagca aatggtggag tctacgaaga aggctatatc attgattcca
     4921 tcacagataa tgcaggtaat gtgatttata aacatgaaag taattctgtc cgtatttatt
     4981 ccgaagcaac ggcatcgatc atgaatgaca tgatgcgtag tgtgatcaat gaaaaaatca
     5041 ctacaccatt caaagatgtc atctcaagct taaacggaaa tctaggaaat gcagattggg
     5101 tcggaaaaac tggttctacc aatgaatacc gagattcatg gctgatcgtc tcaactccat
     5161 ctatcactat cagtagttgg gcaggacacg atgacaatac tggaatggac agccaagcaa
     5221 gaatacgttc ggctaattat cttgccaatc taatcaatca agcttaccaa gcaaaccctg
     5281 agattttcgg cactgatgag aaatttgact tatcatccga tgtgatgaag aaaaaagtat
     5341 ctgggttcac aggacaaaca gctggaaaag ttactgtcaa taaaaaatct atccaaacgc
     5401 caggaaaaac agtggaatca ctttgggcaa aaaatggtcc gaagaaaagc gcctttaaat
     5461 tcggcgttgg cggtactgat gaaaactata cagattactg gaacaccgct tcagcttatg
     5521 ctagagcgaa tccagagacc aaagaatcag acgataaaga tgattgatct aatagattaa
     5581 tcagaattaa agggagctgc gacaaaaatc ttgtcacagc tccctttttc cgaataaatg
     5641 gtgt
//
LOCUS       DAZKNO010000155         5453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000155 DAZKNO010000000
VERSION     DAZKNO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.158"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      <1..71
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            187..768
                     /locus_tag="ACG8MY_002276"
     CDS             187..768
                     /locus_tag="ACG8MY_002276"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0431442.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            771..2078
                     /locus_tag="ACG8MY_002277"
     CDS             771..2078
                     /locus_tag="ACG8MY_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0431443.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2494..2982
                     /gene="purE"
                     /locus_tag="ACG8MY_002278"
     CDS             2494..2982
                     /gene="purE"
                     /locus_tag="ACG8MY_002278"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0431444.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2975..4099
                     /gene="purK"
                     /locus_tag="ACG8MY_002279"
     CDS             2975..4099
                     /gene="purK"
                     /locus_tag="ACG8MY_002279"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0431445.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4112..5407
                     /gene="purB"
                     /locus_tag="ACG8MY_002280"
     CDS             4112..5407
                     /gene="purB"
                     /locus_tag="ACG8MY_002280"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0431446.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
BASE COUNT         1690 a          935 c         1282 g         1546 t
ORIGIN      
        1 tcgtcataat atggatgaca gtttctagcc agtaccgtaa atgctggact ataagtaaaa
       61 gattggctat ttcacccttc ttttcttatg ataaagtgga gggtgttttt ctttaacaga
      121 aacatagtag gtttttgcac aaaagcggtt atttcggtat taataaatga taaaggagca
      181 aataaagtga aagaattagt tgaacgtatt caaaaagacg gacgtatatt aggcgagggc
      241 gtattaaaag tcgacagctt cgtcactcac caagtagacc ctgtattgat ggaacaaatg
      301 ggaaaacggt ttgcagaagt atttaaagaa caaggaatca cgaaagttgt gaccattgaa
      361 gcttctggga tcgcaccagc tttatttgct gcaagagaat tagatgttcc aatgatcttc
      421 gctagaaaag caaaaagttt gacgatggat gaagaattat tgacagcttc tgtttattct
      481 tttacgaaac aagtgacaag tacgatttct atttctcgta aatttttatc ttcagaagac
      541 aaagtattga ttatcgacga tttcttggca aacggacaag cggcaaaagg attgatcgaa
      601 ttatgccagc aagctggagc gcaagtagaa ggaatcggga tcgtcatcga aaagtctttc
      661 caagatggac gtcagttact tgaagaaatg ggattgagag tcgtttctct tgcgcgtatt
      721 gcttccttga cgaatggaca agttgagttt cttgaggagg acgcataatc gtggaaaaag
      781 aaacacaaaa tggaaaagct gcggttttag ggctgcagca tctgctagcg atgtatgcag
      841 gagcagtagc ggtgcctctt ttgatcggta caggactagg ctttaatcaa gaacagatga
      901 cttatttgat ttcaattgat atctttatgt gtggattagc gacattattg caattgacag
      961 ttaaccgatt tttcggaatt ggcttgcctg tcgttttagg atgtgcgatc caagcagtag
     1021 ctccactgat cttgatcgga accaatgaag gagtaggagc tatctacgga tctattatcg
     1081 cttctgggat ctttgttgtg ttagtttctg gtgtcttttc taaaattaaa aaactattcc
     1141 ctcctatagt aacaggaacg gttatcaccg ttattggttt gactttgatt cctgtagcaa
     1201 tcgagaaaat gggtggaggt aatgcaacag cagctgattt tggtgacaaa acaaatctgc
     1261 tacttgcatt tgtgacgatt ctcctaattg taggcgtgca aatgctggca aaagggttca
     1321 ttcgttcaat cgcggtattg attggtttag tcggaggaag catcttagct gctttcatgg
     1381 gaatgattga tctgagcgcg attggagatg cgccgttgtt ccatgttcca caaccctttt
     1441 attttggtaa accaactttc gacgtatggt cgattttact aatgatcatc atttccatgg
     1501 tcagcatggt agaatcaaca ggtgtatatt ttgcacttgg tgacattact gggaaaaatg
     1561 ttggagaaga agaattgaaa aagggatatc gtgcagaagg acttgccgtt atcttaggcg
     1621 gtattttcaa tacattccct tatactggct tttctcaaaa tgtcggattg gttcaacttt
     1681 cgggaatcaa aacaagaaaa cccatctatt tctctgcatt cttcttgatt gttttaggat
     1741 tattccctaa aatcggtgcg ttagcacaaa tcatccctga gccggtactt ggtggaggaa
     1801 tgttagtcat gttcgggatg gtggcagtcc aaggaatccg tatgctgttg gaagttgatt
     1861 tcagcaatga taaaaatcta ttgattgttg ctgtttctgt tggatttggg ctgggtttca
     1921 atgttacgcc gacattattc agtcaaatgc cgcaaacatt gcaaatgttc actggaaatg
     1981 gcatcgtaat gagtagcatt acggctatca tattgaattt gatctttaat ggattgaaaa
     2041 aagatgaaga aatattggaa gaagccttca agaagtaata gagcaaaaga aaggttgtga
     2101 aatcagcgtt ttgacctgat tgttagagaa acaaacggaa aaatagttcc tcgtattttg
     2161 ccgtttgttt gagctaatac cgagggtcag ctgattgaac accgttgaag gaagaggtcg
     2221 tgagcctgcc gttcatctcc gagtcacaag gaacaatttc aaaaaaaagc ttctcatgtt
     2281 tgttgaatat tgttccttgt gaccgaggag ttggcaggag aacaccgttt atccataaaa
     2341 aggaatgtga cctaactttt gtcacattcc ttttttttat ctttaataaa cacgaacaat
     2401 aaaatgtttt tctttgttaa cattcgaaat ttctttgaca tcaaaaaaag tgattgttaa
     2461 aatatgaatg tagaaaaact gggaggaaaa acaatgacag caaaaatctc tgtgattatg
     2521 gggagtactt cagattggga aacaatgaaa gaagcatgtc aaatattgga tgaattcggt
     2581 gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc cagatttgat gtttgaatat
     2641 gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg caggagcagg gggagccgcg
     2701 catctgccgg gtatggtcgc atcaaaaaca actttgccgg ttatcggtgt accggtccaa
     2761 tcacgaacat taaatggttt ggattcgctt ctttcgattg tccaaatgcc tggaggagta
     2821 cctgtagcta ctacagcaat cggaaaagcg ggagcaatta atgctggact acttgcgata
     2881 cagatgcttt ccatgtatga tttagaattg gaggctaaat tggctgaaag acgttcaatg
     2941 ttggctaaaa ctgttataga aagtagtgat caacttggtt aaacctttgt tgcctggttc
     3001 aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg atggcgatta gtgcaaaaga
     3061 aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat tgtccagccg cacaagttgc
     3121 agattggcac attgaaagtg tctatgatga cacctttgcg ctgaaagagc ttgccagaag
     3181 aacagatgtc attacctatg aattcgaaaa cgtcagtgta gaggcgttaa atgcgatttt
     3241 acccatgtca tttattcctc aagggacaga cttgcttgcg attacgcaag accgcctgtt
     3301 agaaaaatct tttttggaaa cgaataacat cgtgattgct ccttatgcaa cgatcgtcag
     3361 tccgacggat atccaagatg cgattgacgg gatcggctat ccttgtgttt tgaaaaccac
     3421 tcgaggcggc tatgatggca aggggcagta tgttttgaaa agtcgggcgg atctagctcc
     3481 tgccatggac cttttaagag aaggaacctg tgttttagaa gcatggatcc cgtttgaaaa
     3541 agagatttcg atcatggtgg caggaaatgg tcaaggagat ttcacgactt ttccggtcgt
     3601 ggaaaacatc caccacaaca atattcttca tgaaacgatt gctccagccg cgattgatca
     3661 ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt gcagaagcag tgagcctttc
     3721 aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc gggctatatg tgaatgaatt
     3781 agctcctcgt cctcacaatt ctggacatta ttctattgaa gcttgttcta tgagtcagtt
     3841 cgatgcccat attcgaggta tttgtggttg gccattagga gaaaaagtac gtttgataag
     3901 caatgcagta atggtcaatg ttttgggaga acaactgctg gaaagctatc gcttgattga
     3961 gaataagcca gactggcaat tccatttcta tggaaaaaaa caagcaaaaa aaggtcgcaa
     4021 gatgggacat atcactatct tgacagaaga catccagcaa acgctggaag aaatcaaaca
     4081 aacgaatatt tgggattaga ggagaaaaaa catgttagaa cgttatacac gccctgaaat
     4141 ggggaatatc tggacggaag agaacaaata ccgtgcgtgg ctcgaagtcg agattttagc
     4201 agatgaagct tgggcagaac ttggtgaaat cccgcaagaa gatgtgaaaa aaattcgtga
     4261 gcatgcatca tttgatatcg atcggatctt ggaaatcgaa aaattaacac gccatgatgt
     4321 cgttgcgttt acacgagcgg tttctgaaac attgggagaa gaaagaaaat gggtccatta
     4381 tggtctaaca agtacggatg tcgtagatac tgcttatggc tatctcatca agcaagcaaa
     4441 tgatatttta cgggaagact tgaaacgttt tacggaaatc attggcgaaa aagcaaaaga
     4501 acacaaatat acagtgatga tgggtcggac acacggtgtc catgctgaac caactacttt
     4561 tggattgaaa cttgccacat ggtattcaga aatgaaacgg aacatcgaac gatttgaaca
     4621 tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca gtgggaacgt tcgcaaatat
     4681 cccgccattt attgaagagt atgtatgtaa acaattgggg atccgtccac aagaaatctc
     4741 tactcaagtt ctccctcggg atctacatgc tgaatatttc tcagcgatgg cgcttattgc
     4801 tacaagtatt gaacgttttg caacagagat acgcggattg caaaaatccg aaacacgtga
     4861 agtagaagaa ttttttgcga aaggtcaaaa aggttcttct gctatgccgc acaaaaggaa
     4921 tccgatcggt tcagaaaata tggctggtct agccagagtg gtgcgcggac acatggtcac
     4981 tgcctatgaa aatgtcacat tatggcatga aagagatatt tctcattctt cagcagaacg
     5041 aatcatcttg cctgatacca cgattttatt gaattacatg ttaaatcgtt ttggcaatat
     5101 cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg aatatgtctg caacatttgg
     5161 tttgatttat agccagcgcg ttatgctgaa attgatcgat aagggaatga caagagaaga
     5221 agcttatgat cttgttcagc caaaaactgc gtacgcttgg gacaaccaag tgcagttccg
     5281 cccgctgtta gaagaagatc caaccatcac atcggtatta tctaaagaag aaatagacga
     5341 tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg atattcaaac gagtaggttt
     5401 ggaataaaga agagtgagtt gcaaaattca attaaaatag aatagcaaaa ata
//
LOCUS       DAZKNO010000156         5437 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000156 DAZKNO010000000
VERSION     DAZKNO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.159"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1138
                     /locus_tag="ACG8MY_002281"
     CDS             <1..1138
                     /locus_tag="ACG8MY_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431447.1"
                     /translation="DLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILL
                     ALVRYVNWEGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKA
                     FPLGLKEEDKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENME
                     LLRKDFEVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQV
                     FLPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAG
                     RQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVL
                     CDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1359..3122
                     /locus_tag="ACG8MY_002282"
     CDS             1359..3122
                     /locus_tag="ACG8MY_002282"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0431448.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3206..3664
                     /locus_tag="ACG8MY_002283"
     CDS             3206..3664
                     /locus_tag="ACG8MY_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0431449.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3773..4456
                     /locus_tag="ACG8MY_002284"
     CDS             3773..4456
                     /locus_tag="ACG8MY_002284"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0431450.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4603..5217
                     /locus_tag="ACG8MY_002285"
     CDS             4603..5217
                     /locus_tag="ACG8MY_002285"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0431451.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5233..>5437
                     /locus_tag="ACG8MY_002286"
     CDS             5233..>5437
                     /locus_tag="ACG8MY_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431452.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         1935 a          851 c         1114 g         1537 t
ORIGIN      
        1 tgatcttgaa tttatttctt tgagcaaagt atatacaatc aaaaaaatcg tattggattt
       61 ttttaaagaa aatgattatt tgaaaaataa agaaatcatt atcccagaat ttgactcgcg
      121 gaacatcttg ctagctttag tacgttatgt caattgggaa ggctatgaaa ataaaaatcc
      181 gtatgtagaa gaagcttgta acgagttgat tgattatgta gagactcatt tttttaaaag
      241 aagatattcg gaagatgaaa agtcctttat catacgagga atagagatcg caatcggaag
      301 aaaggcattt ccccttggac tgaaagaaga agataagaaa agcgcagaaa cgaagccgtt
      361 atttcaatta gtctgtcaag gattggaaga gcaaaaagga attctagact tacaagaaga
      421 agatgtctat tatatttttt ctttgttcaa cagtagaaat tataccaatg agaacatgga
      481 gctgttaaga aaagatttcg aagttgttta taaaaatttt gttctcaatg acctatcact
      541 gaatgaattg attttacaga ttattagtat cgctgaaatc gacgatgcag aaaattttat
      601 tttcaagcaa tcctttttac aatttgtccg tacgttgtgg gcagatggac aagtattttt
      661 accggaaagg atctatcttc tatcagaaaa agaacaactt ctgtacggaa aaattgtaga
      721 aatattggaa gagtggaaaa agaaaaatca aatccaacta agatggaatg aaaatttcat
      781 tcgaaaattc gtcaaaagtc tttctttgat caacagtgaa caagcgggga gacaggaaat
      841 agaaattttt gttgtttcag aaagttcagt caaacaattt ttttatcgaa cccaatttca
      901 tttgtatgcg gaagaaggca tcgctgaaat aaatcctatt ttatttcgtt cattagaaga
      961 attgcctgat gaatgtctat gtgcgaaaaa aagagtggtt ttgtgcgatg ctgctagcta
     1021 tcaaaatgga ttagaaactg aaaagacaaa gatctatcca atctcattaa aaggtgcaaa
     1081 catccatttg tatcatattg ctcaggaact tcacctatta aaaaaacata tttcttgatg
     1141 agaccttaat taggagacct tctacaaagc tttttaattg tctgtagaag gtttttattt
     1201 gagtcctgat tcgtgcgaag atctacttat ataacaagct ttctctgaaa tttttttaaa
     1261 atgctattga cttagagtca actctaagta gtagaataga taaagtgtaa caaatctatt
     1321 ttcattaaaa gaagatagta cggaggagag aatgtattat gagtacagta ttacatgtat
     1381 tggatgcaat caatagcccc gcagatgtca aaaaattatc tgttgaggaa atgaaacagt
     1441 tggcagaaga gatgcgccaa aaaatcttgc aaaaggacag cgatgtaggt gggcacgttg
     1501 gtccgaatct aggtgtagta gaattaacga ttgcgtttca ttatgtattt gattcaccaa
     1561 aggataaaat cgtttgggat gtttcccatc agtcttatgg tcataagctt ttaactggtc
     1621 gaaaaaatgg ttttgaaaaa ggacattacc gtgaagtgag cggatattcc aaccaacatg
     1681 aaagtaaaca tgactatttt acagtggggc acacatctac atctatcagc ttagcaacag
     1741 gtttagctaa agcgagagat ctgaagaaag aatcaggaaa tgtggtagca tttatcggtg
     1801 acggctccct aagtggtggc ttagcttttg aaggattgaa caatggtggt gttttacaat
     1861 ccaatctaat cgtattagta aacgataatg agatgtctat tgatgagaat catggcagtt
     1921 tatataaaaa cttagctgag cttcgtcaaa ccaacggtaa aagcagcaat aacctattta
     1981 aagcaatggg atttgattat cgttacgtgg ataatggaaa cgatctggaa acggtgatcc
     2041 gtttatttga agaagtaaaa gatattgatc atccgattgt acttcacgtc catacggaaa
     2101 aaggtcgcgg ttatcagccg gcaatcgata ataaaatgaa atatcattgg catgtaccat
     2161 ttgatctgtc caccggggag gcaaagcaag taacttctgg agaaacattt aatatgatca
     2221 tcatggatta tttggataag aaaatgcaaa caagtacagc tcctattgcc gcagtcaatg
     2281 cagcgatacc aggcgccttt ggactgaaaa cattccaaga aaaacatcca gatcattatt
     2341 ttgacgtggg gatcgctgaa cagcactcta tcactttcgc ttctgctttg gcttctcagg
     2401 gagtacgtcc agtcgtcttc caatcaagta ctttcttgca acgggcatat gatcaattat
     2461 cacatgattt agcgatcaac gagaatccag tgattatttt agtcggtggc ggaacgatct
     2521 cagctagtga ccgaacacac caaggaacat ttgacattcc ttacttatca agtatcccga
     2581 acatcaaata tcttgctcca gcaacaaaag aggatatgat ctctatgcta gattgggcgt
     2641 tcgaacaaac agcgcagcct gtagcaatcc gaattcctac acatggtgta gaacatggta
     2701 cagctatcgc aaatgattat agtattccag cctatcaaac agtaaataat ggaggaaaag
     2761 tagcaatttt agctttaggc ggttttcttg aattaggaaa agaaacagta aaagctttta
     2821 gaaaaaaagg aatcgaagca acgttgatca atccaggaac gatcacagaa aatgacgtag
     2881 aaacattaga taaattaaaa gaaacacacg aattagtcat cacattagaa gatggtagtt
     2941 tgtcaggagg atttggcgaa aatatcagtc gttattatgg tgattcttca atgaacgtat
     3001 taaattttgg tgcagagaaa gaattcacgg atagcgttcc tgtagggcag ctttatgaac
     3061 gttatcattt aacaccaaaa caaatcgttg aagatagtct gcgtgtcttg aatattcaat
     3121 aattcattat gctcaccatc cagataattg tttatactag tatctggatg gttttttcat
     3181 tataaaaagc aaaggggtgg atgttatgta tcagcaagta gaagaatatg tcaaaaagtt
     3241 ccaagaaaaa tatcctagtt atcaatcaga aaaattaggt ttcttttcat ttggggatac
     3301 agaaaaaatg gcagacgagt tgagcgaact agttgtaaaa aagataaaga ctggtacatc
     3361 aagtggttac gatctgtata aacaagagaa tgagccttta ccaaaagttg gtcaattaga
     3421 cgtggtcctg aatggaaaaa atgaaccagt ttgtatcatc aaaaatataa atgtgaccat
     3481 cttgcctttt agtgaagtca cgaaagagca tgcttggaaa gaaggagaag gggatcggac
     3541 attagcttat tggcggaaag tccatgctga gttttttaaa catgcttata aagaagcgtt
     3601 gaacatagaa tttacagaag agaagctggt cgtatacgaa gaatttgaag taattttctt
     3661 ttagtaagaa aggtctgctg aaagaaatag ctcattattg tctcctgaat agaaaagggt
     3721 tacattttta tatgaagctg tatttttata taacattact ggaggagata ttgtggatac
     3781 actggaacaa atcaaaggcg gagtcgtcgt ttcttgtcaa gctttggaaa acgagccgct
     3841 acacagttct tttattatgg ggagaatggc tttggctgca aaagaaggcg gagcagttgg
     3901 tattcgagca aatacgacag aagatatcaa tgaaatcaag cgaatcgttg atttaccggt
     3961 aatagggatt atcaaaagag attacgatgg gtcagaagtg tttattactg cgacagaaaa
     4021 agaagtggaa gaacttttgg agacgcaagc agaaatcatt gcactggatg caacttctcg
     4081 taaacgtccg acagaaaaaa caactgctga acttgtccaa atgattcacg aacacggtcg
     4141 gttagctatg gcagatattt cgacttttga ggaagcgctt caagcgcaag aagatggttt
     4201 tgatcttttg tctacgactt tagcaggcta tacagaatat tcacgaaaga cgcagacacc
     4261 tgattttgat ttattgaatc aaatcataga acaagtcgat atcccagtaa ttatggaagg
     4321 acatactgac tctcctgaac aagtagagaa agcatatgaa ttaggtgctt tttcagtcgt
     4381 agttggatct attatcaccc gtccgcaatt gatcactaaa agatatgtcg aagccgcaag
     4441 taaagcaaat aactaaaaaa cacgcacggc acagattctt gacaaaaaag aaaaattgat
     4501 agatagttta aagcagattc taagaagatg aaaaaagcat tacttttcat tttgtcttca
     4561 agatctgctt tttaacgaat tttatagaaa gaaggcgaaa ccatgtttga acggagtcct
     4621 tcagaattga cagaacgtga aaattacaag ttgctgattg gttcagtcat ccctagacca
     4681 gtagcagtag taacgactaa atcagcaaaa gatatagtga atatcgcacc cttcagttac
     4741 ttcaatatcg tcagttcgaa tcgacccatc ttatccttgg cgatccagag aaaacaagat
     4801 gcagtaaaag acacagcaaa gaatattctt gagacaagag aagctgttat ccatatcctt
     4861 gatgaggaca acgtagaaga agtaaataaa acagcagcca atctgcctaa tgaggaaagc
     4921 gaactgtacc tgactgactt gactttaact gattcagtaa tcatttcagt accagcattg
     4981 acagaagcaa aagttcgttt tgaaacatcc ttatatgaac atgtcgagat acggaataaa
     5041 aataaagtaa cagctgattt atttttgcta gaagtacaga aatatcattt aaatgaaaca
     5101 gtctatgatg ataagacagg aaggatcgat ccaagaaaat tgaatgctgt aagccgtttg
     5161 gcaggaaatg attatgcagc aataggagaa attttcacga ttgcacgtcc cgaataaaaa
     5221 ggagcactag ttatgttaaa aataagctat aaaccatcga cagattcaaa agaaatgaaa
     5281 aaagaatatg aaacagtgaa tgattttctg caagggcagt acttagaagt tccgccattg
     5341 caagaccatt ttgtcgttac tactgttact ttagatggta aggaaataga aatgccagat
     5401 caaacgatta gcggattgtt taattacttt aacaaat
//
LOCUS       DAZKNO010000157         5381 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000157 DAZKNO010000000
VERSION     DAZKNO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.161"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..1371
                     /gene="tig"
                     /locus_tag="ACG8MY_002287"
     CDS             82..1371
                     /gene="tig"
                     /locus_tag="ACG8MY_002287"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0431453.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            1618..2868
                     /gene="clpX"
                     /locus_tag="ACG8MY_002288"
     CDS             1618..2868
                     /gene="clpX"
                     /locus_tag="ACG8MY_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0431454.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            2951..3538
                     /gene="yihA"
                     /locus_tag="ACG8MY_002289"
     CDS             2951..3538
                     /gene="yihA"
                     /locus_tag="ACG8MY_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0431455.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(3612..3767)
                     /locus_tag="ACG8MY_002290"
     CDS             complement(3612..3767)
                     /locus_tag="ACG8MY_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0431456.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            3903..5231
                     /locus_tag="ACG8MY_002291"
     CDS             3903..5231
                     /locus_tag="ACG8MY_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431457.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         1868 a          895 c         1105 g         1513 t
ORIGIN      
        1 aaagggttag tgtactattt aaaggtatgc atgaaacgtt tttgatgtat aaaatgaatt
       61 tctaaactcg gagggaatta aatgactgca acatttgaaa aaaaaggcac gaatgatggt
      121 gttttaaaat ttacgattgc gcaagacgaa atccaaaaag gattaacaac tgcgtttaat
      181 aaagtgaaaa aaagcttgaa cgtacctgga ttccgtaaag ggaaagtttc tcgtcaagta
      241 ttcaaccgta tgtacggtga agaagcttta tatgaagatg cattgaatgc tgtattacca
      301 gaagcttatg aagctgctgt caaagaagct ggcatcgatc ctgtttctca accaaaaatc
      361 gatgttgaaa gcatggaaaa aggtcaagac tgggtaatca ctgctgaagt aacagtgaaa
      421 ccagaagtaa aattaggaca atataaagat ctagttgttg aaaaacaaga ccgtgaagta
      481 acagatgctg acgtggatgc acgtatcaaa cgtgaacaag aagcacaagc tgaattagtt
      541 atcaaagaag atgctgcagc tgaaaatggc gatacagtag taatcgactt cgaaggcttt
      601 gtagatggcg aagcgtttga tggcggaaaa ggcgaaaatt actcacttga attaggttca
      661 ggctcattca ttccaggttt tgaagatcaa ttagttggtc ataaatcagg tgaaagtcta
      721 gatgtaaatg taactttccc agaagattat caagcagaag atcttgctgg aaaagaagca
      781 gtcttcaaag tgacaatcca tgaagtaaaa gcaaaagaag tgcctgaatt agatgacgaa
      841 ttcgcaaaag atgttgatga cgaagtatca acattagacg aattgaaaga aaaatatcgc
      901 aaagaattag tagaaacaaa agaaaaagca gcggacgatg caaaagatga agcagcgatc
      961 cgtatggctg ttgaaaacgc agaaatcgtt gaattgccac acgtgatggt tcatgatgaa
     1021 gtacatcgtt caatggacga attcttgaac aacatgcaac gtcaaggtat ctctcctgaa
     1081 atgtactatc aattaactgg ttctacagaa gaagacttgc ataaacaatt cgaaggtgaa
     1141 gcagaaatgc gcacaagaac aaatcttgtg atcgaagcaa ttgcagctgc tgaaaatctt
     1201 caagcaacag aagaagaaat caatgcagaa atccaagaat tatcagaaac atacaacatg
     1261 ccaatcgaac aaatcaaacg cgtgttgaca gaagatatgc tggaacatga tgtgacaatg
     1321 aaaaaagcaa tcgaattgat cacaggtaca gcgacagaag cgaaagaata gaaatcaagt
     1381 aataaatcat ttctgaagaa gctacttcta gaaaattgtt tattcgaaat aagggactgt
     1441 gacacgattt tgtcacagtt ccttattttt tgttaagagt aaaaaatgca aaggtatagg
     1501 tcagttgatc ctttgttaaa cactttttct ttttactaaa ctatgttatg atagtaaaag
     1561 actgtaaagt ctttgatagt ttcttgcata ctttttgcaa gttaaggggt gaagaccatg
     1621 tatgacaatc caagtagcaa tgagaccgta cgctgttctt tttgcgggaa aacccaagaa
     1681 gaagtgaaaa agatcgtagc tggtccaggg gtctacatct gtaatgaatg tatcgaccta
     1741 tgtaaagaaa tcattgatga agaattttat gaagaagctg tacgcgaatt cactgaagtc
     1801 cctaaaccac tagaaattct tgaagtattg aataactacg tgattggaca agatcgcgca
     1861 aaacgctcgt tagctgttgc cgtatacaat cactataaac gtgtcaatca gcaagcacaa
     1921 gaaaacacag atgatgttga actgcaaaaa agcaatatct gcttgatcgg acctacaggg
     1981 tctggtaaaa catttttagc acaaacatta gcaaaaacat tgaatgtacc ttttgcaatc
     2041 gcagatgcga caagtttgac agaagctggt tatgtggggg aagatgtaga aaacattttg
     2101 cttaaattgc ttcaatctgc tgattacaat gtggaacgtg cagaaaaagg gatcatctat
     2161 attgatgaga tcgataaaat tgcgcgtaaa agcgaaaatg tatctattac tcgtgacgtt
     2221 tccggcgaag gtgtccaaca agccttgctg aaaattctag aaggaactgt tgcaagcgtg
     2281 ccacctcagg gaggacgtaa acatccacat caagaattta ttcagattga tacgacgaac
     2341 atattgttca tcgtaggtgg tgcatttgat ggcatcgaaa cgattgtgaa gaatcgtatg
     2401 ggcgaaaaga cgattggatt tggtacacaa aaccaaaaac tttctgaaga cgaaagtgtc
     2461 atgcagcata tcattcctga agatttacta aaattcggat tgatccctga gtttatcgga
     2521 cgattacctg ttatggcagc tttagagaaa ttgacaacag atgatctggt acggatcttg
     2581 actgagccga aaaatgcgct agtcaaacaa tatcaaaaac ttctttctct agataatacc
     2641 gaacttgaat tcgaacctga agcgttgaga gccattgcga aaaaagcaat cgaacgcaat
     2701 accggtgctc gaggacttcg ttccatcatt gaagaaatca tgatggatgt gatgtttgat
     2761 atcccttcaa atgaaaacat cgaaaaagtg attatcacaa aagaagcagc tgaattgtct
     2821 ggtgagccaa cagtaatcta taaagattca gagaaaaaag cgggttgatg aaaagaggct
     2881 gccgtaaaag tcattttggc ttttgcgaca gccgcttgct ttattcatca ccttgttaag
     2941 gagagagacc atgaaagtcc atcaagctga aattatcata agtgcagtgg caccaaaaca
     3001 atatccagat acacaattgc cagaaatagc tctggcagga cgatcaaatg tagggaaatc
     3061 ttcttttatc aacacattga ttgatcgaaa gaatttagcc cgtacttccg gtaagccagg
     3121 aaagacccag acattgaatt tttatttgat cgagaacgca ttacattttg tcgatgttcc
     3181 gggatatggg tatgcaaaag tatccaaaac tgagcgagca aaatggggaa agatgatcga
     3241 gacatattta acgactagag accagctacg tgcggtcgtt tctttagtag atttgcgtca
     3301 taagccttct caagaagata tccagatgta tcaatttttg aagtattatg atatccctgt
     3361 aattgttgtt gccactaagg cagataagat ccctcgtggt aaatggaaca agcatgagag
     3421 cgaaatcaaa aaagcgttgg attttgatcg caatgacacg tttattctct tttcttccgt
     3481 tacaaaacag ggaaaagaag aagcgtgggc agcaatcgaa caagcaattg aatcataaaa
     3541 agaataaaaa agacttacca gtcaaactct ttcataaaag tttggctggt aagtcttttt
     3601 ttgagcttaa cttacttttg tttcttgtct tttttctttt cattttgtgg ctgtggtttt
     3661 agaaatcgat cgatctcttc ttttggtgca actgtttttt ccggatcgac agcaaatgat
     3721 tcgaaaagtt tatctaaatt atctgtacgt tcaaatttca accccattgt aaacacctct
     3781 ttgtttcttt ttacttagtc tatcatgtaa tcgtccaaat tggtttctaa gacataaagt
     3841 tttagttttt ctttatcgtt ggttataatt aaattataaa aagattttaa agaggtgaga
     3901 aaatgattgg cgaagataaa ttattgatga ctatccagct cacagactta aatgaagaag
     3961 aaaaaaaaca ggaaataagc gattgggcta ttttgacacg tctgttgatc cagccaacat
     4021 ttatccgctc tttcacacaa caagcggatc cgtatgatct tcgaaagctt caagaaaaaa
     4081 taatgctagc atcggtaaga gacgaaagtt ggttagttgt aggaaatgac gagaacgaat
     4141 gtagttttcg cttggaagat aatcaattat tgataaaaaa tgtattgtct atctctgttt
     4201 tcaaagaaaa gcaatttttg attcgagact tcattcagaa aaaaatgatt gctcatgggg
     4261 tgttcgccta tatgcgagct tatagcgagt ttatctatca caatacaaaa cagatctctc
     4321 aacggctatt gtttgagaaa aaggatgaaa tcgaacattt acctaaaatg aaacaacaaa
     4381 acggggaagt agtagtcgat tgcaaccaat ttccaggata cgatcttttt taccaaggtt
     4441 tatgtttcac ttcgtgttgg gaaatgtatt atagtcgcta ttatcatcaa atcatcccta
     4501 aaccgatttt tctcgacatc caacaagtag aaaaagtgaa ggaattagaa aacgaagtca
     4561 tatgtatcca gctttatcga gaccccttta actggcaaaa gccaaacaat caaatgtttc
     4621 aatcctattt tagggaccag cttgggtttg atcatcttgc ttgggacaat ggtgtcggtt
     4681 tattgaaacc gccattcgtt gaatacattt acactgacca tacgatccaa tctgtccagt
     4741 atcagaatgc acaaatgcaa cctgttccta aaaagaatgc ttcatttttt gtaacaaaaa
     4801 gttatgatat tcaacaagga gattataaag aaaggcgtgt tcgcggaaca ctaaatgctc
     4861 aggcgtattt tccttgggtt gacgagaatc gctcgcgcat gatgtgctat aaagttatcg
     4921 atccgactgt tgctttggat aatggaatcg aagcttattg ttattatatt agggaatatc
     4981 tagaagtaga ggtaacagat gaaaaatatc aagagtatct tttatcctta agaatctatg
     5041 ttccttcaga atccctgtca gaattaccgt taaaggaaat caaacataga ctctctgatg
     5101 ttaccttcaa acgaataaag aaaaagcggc gaagtatcca attcgatgtc aagaaaggtg
     5161 agcagcatct gcgtgtgcaa tttttagatt accgagaatt agaacaattg attactttac
     5221 aaaaaatatg aggtgaagaa aatggataaa caactctatt atcgaataat ggttccggca
     5281 tcattattat ccgtctgtct cttttttgtt cctaattttc ctattcgatc agtcatcggt
     5341 tttttggtca tgtttggcgg gatgggactt tattattatc t
//
LOCUS       DAZKNO010000158         5342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000158 DAZKNO010000000
VERSION     DAZKNO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.162"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..291)
                     /locus_tag="ACG8MY_002292"
     CDS             complement(<1..291)
                     /locus_tag="ACG8MY_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431458.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYI"
     gene            complement(589..1563)
                     /gene="sgrA"
                     /locus_tag="ACG8MY_002293"
     CDS             complement(589..1563)
                     /gene="sgrA"
                     /locus_tag="ACG8MY_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0431459.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1759..2598)
                     /locus_tag="ACG8MY_002294"
     CDS             complement(1759..2598)
                     /locus_tag="ACG8MY_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0431460.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2783..3670
                     /locus_tag="ACG8MY_002295"
     CDS             2783..3670
                     /locus_tag="ACG8MY_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0431461.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4264..4959)
                     /locus_tag="ACG8MY_002296"
     CDS             complement(4264..4959)
                     /locus_tag="ACG8MY_002296"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0431462.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4943..5341)
                     /locus_tag="ACG8MY_002297"
     CDS             complement(4943..5341)
                     /locus_tag="ACG8MY_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0431463.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1706 a         1078 c          782 g         1776 t
ORIGIN      
        1 aatataacca atcgttccct ctaaaagtat agaataaact ttactcagta aaaactgaga
       61 agaaaactcg acttttatca tcttgttttc tgaaagaaaa gataatttat attctggata
      121 cctttcgcaa aacgattctg cttgttgaat caccgaattc atcttagcca gggtgtagtt
      181 tagatctaaa gcgatatcct tcaccacata taatttctcg tctctttcat aacaatagtt
      241 caaaaattga tatacttgcc tttcattgat atcaataaat tcttcaaaca aagtacatac
      301 tcctttttat ctattattta gacttattcc gacagctaca tgaataacgt acaaatagat
      361 tcgttactga tttttcagag atactaccat gcgcagtgtg cattgtcaat tattcttgct
      421 cttattttcc atttgtgttc ctgttatttc ctgtttctca aatatgttag taaacaaaaa
      481 tagatctggc aaagctacaa cttcgccagg caatacagca ctctcgaatg aaatattcac
      541 tcaaaaatct atcgttttat tcaattttag atctgttgct tttattcttt atttcactcg
      601 atggaagaga gaacgaaaat agcttgctcc tacaatcaaa atgagtaaca cacccatcca
      661 tgtaattagc ttgttcaagg ttctactacc agtttctggt aaatcgcttt ttctattatc
      721 tgtttctttc gctttttctt gatgatcaat tccacgagaa acaaaatcag gctgttttag
      781 cacacttttt aggttgctcc ctgcatttct cttttgttcc ttagttggta tgataggtac
      841 caaatcaagt tcattattta cttttcctgt gctgctttcg ctgctcgtgc ttcctgtgct
      901 actctcacta ctcatgcttc ctgtgctact ttcactactc gtactctccg tactgctttc
      961 gctgctcgtg cttcctgtgc tactttcact actcgtactc tccgtactgc tttcgctgct
     1021 cgtgttttct gtgctacttt cactgctcgt actctctgta ctgctttcac cgctcgtgtt
     1081 ttctgtactg ctttcactac ttgtgctatc cgtactgctt ggcggtccaa caactgtcca
     1141 actgcttggt ttcaaattct ccgtgatcgt catctgtcca tcataaaaaa caatcaaatc
     1201 aactttatct ttactcttga tctcatcaat cgatagttct gcagaaatca ttattgtata
     1261 ctcaatacca gcggtccatt gtacgtcttt taaataaact tccccaataa ctttttctga
     1321 acttccatta ccatcccaac catatgttag aacagcatgt acatcactag cattcacgct
     1381 tttgtttggt ttgactggaa tagtaatgac ttgttgtgtc atgtcataaa caatccgatc
     1441 agttttcaca gtaaatgaaa gattctcatt tgcccgttca tttgcagaag tactgattcc
     1501 aatcattgct gcaattatac tcagcccaag aataacaaat aatacggtcg ctgttttctt
     1561 catttctatc actctccata taattacctt ctatttgata aaggaattat ataaaatgaa
     1621 aatgatgaga acacgacagt cagttgtgaa aatctaacaa aagatgtcgt gtattcgctt
     1681 gaatttgttt actttttcgt ttaatcaatg acagacttaa aaaagcaaga ccgatatcag
     1741 ccttgctttc ttcatctttt attgataaac agcagtgtat ttcttcatcc gctttgtttt
     1801 atatgcatag agtaacaaac caacaatcaa gactaacaag aataatgtag ttgatataga
     1861 tgcttcagcc ccaaagtttc ccccagtaat tattgtattt tgcgattgga tcagagtgga
     1921 gaaaaaacct tgttcttgcg ttccgctgac tggtataccc aaaatagctc cttgtgtcca
     1981 attccaagta atatgagcac caatagccgt aaataaactt cctgtactgt agataagtgc
     2041 gctgaaaaag caacctgcta aaaagatatt gaaaactgcc agaaatgtca tgttaggatt
     2101 caatccgtgc aaaagagcaa ataatgcagc agatccaatc attgctatta acggaccaaa
     2161 tctaagcatc tgatgctgaa aaaagccacg gaataaaagc tcttctgatg cccccactgc
     2221 aataaataaa ataaaactcg ttatcagcgg cacaatgttt gcgtttcctg aatctaaccg
     2281 atacgccccc gtcaaatata atggactagt aataagcaga aacactccaa ttccaacaac
     2341 gagacctccc gctaaatacc cccagtcttt tcctcgaaca gaaacaccat aatcttctat
     2401 tctttttcct tcgaccttac tagcaagaaa taaagctagt aaaaaaacag ctaaatgtcc
     2461 gaccgtaccg gcaattgctc caatccattc actatttcca ataaacatat ccgcaagtgc
     2521 attattgaca agaattccta aaacaaaacc aactaagact attaaaacag atccaatcaa
     2581 ccgaataatc ctgatcatct ttcatccctc ctttatatta agtttaacaa cccttttctt
     2641 tcttgtctcg tgaaaactat gtaaatgttc tccatcatag agagaataga tttttacgaa
     2701 ctaagaatat ctacagcaac cttacgaaaa tgaaagactg tctccctaat tcttctgtta
     2761 gactgttttt aataaggaaa ggatgggaaa ttttttgaaa aaacaactac taattatgtg
     2821 cctttcagca tgtgcaattt tactattctc aggttgtagc aagtccaatg aaacgattgc
     2881 aacgtttaaa ggtggaacac ttacagttca ggatttttat gataacccaa aagttaaatc
     2941 aaataatcaa acactattaa aaaaaatgat tgtcttcaaa gcatttgatg atgtatacgg
     3001 aaaagagatt tcttcaaagg aagtaacaaa agaatataat gaacaaatca aaaaactcgg
     3061 tccaaactac aaggaacaat taaaagcagt tggccaaaca gaagagacat ataagaaact
     3121 ctttaaacag atgctggctt tccaatacgg tttgaaagcc aatgtaaaat taacagataa
     3181 agatctagac actgcttgga aagaattcta tccagaagtt agtacacaga tcattctatt
     3241 ttcgactaaa gaagaagcag ataaagcaaa aaaagaagca aatgaaggag aaaatttctc
     3301 caagcttgtt caagcttatg gaaaaaataa aacattaaag gaaactgatg gaaaaatgaa
     3361 ttttgattca accaatccag agattcctac tgaagtaaaa aaagcagcat ttaaactaaa
     3421 aaacggggaa gtttctgata tcattcctgt aacagatcca acgacttatc agcaaagcta
     3481 ttatctagta aaaatggtaa aaaaacagga taaaggttct aataaagaca aatacaaatc
     3541 tgagctagaa aaaattgcta ccgaagcaaa aactcttgat acggaattca tggataaaac
     3601 aatccgtaaa gtgatgaaaa aagacaatgt aactattaaa gatccttatg taaaaaatat
     3661 attcaaataa acaaatttga cccaattggg gatgtggcaa aatttgagaa gtgatttttg
     3721 gaaattcctt cttatttttg aggcaaacca cttttgccac aacttcatcg gggccttttt
     3781 ttatttgtac caaaacaaat cactggattt gttacaatca tgaggatata atttaaaaaa
     3841 gttttttatt tctatcacat gaatcaatgc gccattaagt agattctttc atgcttccct
     3901 attttttcac ttgccgtatc gcttcaaaaa caactatcat aaactcaaac tcgactgaaa
     3961 agttatttta aaaaagacta aaaatatccc ttctggactt gttcttctta ttctgttata
     4021 ctagagaaac acgaaagata tttttatgcg acacttttca tttgttttta tgaaatttat
     4081 tcaaaactgg cgtaacttca gagttacaag gatgtattag aggttaggga atacatcgtt
     4141 taaatgagaa ataagtaata gttagtaata gtcacccaaa ctttaaatta atgaatattg
     4201 aatcgtcaga tagcctcctc gagttttcga gaaggctatc gtttatttta tggagaaatt
     4261 gattcagaag taatcagcga ccttttttag ttcaccaatc gtttctgctt ggaaagtttg
     4321 cacccaatct tcttggaagc ccctcactgc tttatcaatc gagggcatct ctaatacttg
     4381 atctagtagc tctttaccta acgttatcgc atgagctcca gatcgaatgg ctgtagtgac
     4441 ttgttcacaa tttttgaaac tagctgctaa tatttttgtt ggtctttgcg tacgttcgat
     4501 ctcagtccgc aacgaatcaa tcactttcat agcatcaatg ccttgatttt gcatcctgtt
     4561 ataataaggt gcaatataat ctgctccatt attaatagct aaataccctt gtaattctgt
     4621 gtaaatagct gtaccggtaa tcaaataacc ttcttttttc aactttttga tcgcttgaag
     4681 gcctgcttga ttcaccggca ccttgataaa cgtatctttt cccagttctt ctatgataac
     4741 tcgagcatcc tctatcattt cttcttccgt tcttcctatt gtctgaatat ggataggaat
     4801 ctgtccgatt atttttttga tttctttcat atgcgagaag aagtttgacg ctccttcttt
     4861 tttcagaatt gtaggatttg ttgttacgcc tgttaaattc aaaaactgtt gacaagaata
     4921 aatacttttt gtatttgctg tatcaagcat gatttccata tagctttccc ccattctgat
     4981 cctcttgatc gattttaatg attgtcccct ttcttaaatg cggcatttcc ttatcttcta
     5041 catacattgt tcctggaagt tccggctcat gagatccatc aaattttatc gtgatatgtc
     5101 ctaattcatt caaattagtt tggacaacat ctcctactgc tgtgattaca tacatttgtt
     5161 tatcaaaaca gagcttcata tcacaagtta tttcttttct tacctgattc aattcaatct
     5221 tataacaata atctgttaat gtttccggtg cttcatctcc aaataaaatc attatttttt
     5281 ctttcctgaa tacttctgct tctataccaa catctgttac aaccgtttca aagatcgtca
     5341 tc
//
LOCUS       DAZKNO010000159         5327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000159 DAZKNO010000000
VERSION     DAZKNO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.163"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(265..378)
                     /locus_tag="ACG8MY_002298"
                     /pseudo
     CDS             complement(265..378)
                     /locus_tag="ACG8MY_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(761..1993)
                     /locus_tag="ACG8MY_002299"
     CDS             complement(761..1993)
                     /locus_tag="ACG8MY_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0431464.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2248..2817
                     /locus_tag="ACG8MY_002300"
     CDS             2248..2817
                     /locus_tag="ACG8MY_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0431465.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2995..3435)
                     /locus_tag="ACG8MY_002301"
     CDS             complement(2995..3435)
                     /locus_tag="ACG8MY_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0431466.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3593..4357
                     /gene="map"
                     /locus_tag="ACG8MY_002302"
     CDS             3593..4357
                     /gene="map"
                     /locus_tag="ACG8MY_002302"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0431467.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4389..5312
                     /locus_tag="ACG8MY_002303"
     CDS             4389..5312
                     /locus_tag="ACG8MY_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0431468.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1653 a          970 c         1036 g         1668 t
ORIGIN      
        1 tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt gtgaaggcgg
       61 aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg ccaaaaaaat
      121 tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt cctgcaataa
      181 caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc cctgtatcct
      241 ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac tgctatgtcc
      301 taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgcgt
      361 atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga ttactaaaga
      421 aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc gaattaaacg
      481 atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt aactctagcg
      541 gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac tttttattat
      601 ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta ccatataaaa
      661 aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga tcctttattc
      721 tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc
      781 ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc ttgtcagatc
      841 ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct aattcttcat
      901 cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt gctaagtgat
      961 tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga gaaataggtg
     1021 aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct gtatcaagca
     1081 gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aacaactttt ccatctttaa
     1141 aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt gtacttgaaa
     1201 tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg
     1261 ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga tggtttttag
     1321 gcaatccgat aatgatatct gtaccgggag cagtatagtg aagggcagaa aattgttctc
     1381 gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat gctaagacag
     1441 ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc tcaacttgtt
     1501 tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc gcagccacga
     1561 cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt gctttgccat
     1621 tcgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca tcgggatcgg
     1681 cagaaacaac gctgatcctg cttgctcctt tttcaagcca ttcatccgct tgatcgattt
     1741 tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat tctctttgga
     1801 tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa gcttcttgtg
     1861 tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact gtgtgtcctt
     1921 tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga agttcattaa
     1981 aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta ttagcatgat
     2041 accatttaat tatgattcaa acaatgaggc tttaaataaa aaaaggaggt tattttttta
     2101 aggttaagtt tcatgatata tgttactact tgctatttca gcttgtgaag aaaatcaaaa
     2161 actaattttg catatcaaaa aaaacatgtt acaatgaaat taatcaatgg tggaagcgtt
     2221 ataaaaaaat taaggagatt aaaaaatatg gcaagaaaaa agacgattac tagagaacag
     2281 attttggcag ctgcttatga agtagctgcc accgatggat ttacacgatt tactgcgaga
     2341 aacatcgcga acaaaatgaa ttgttccacg caaccgattt atcttgagtt taaaaatatg
     2401 gatgacttga gacaagctct ttttgagcag atctatgatc atttacaata tgaagtgttc
     2461 actgttgagc atacagggaa tacgattatc gatcttgctt ttaattatat ccattttgca
     2521 agaagagaga agaagttgta tcgttcactg tacttagaag aatatggtgg aggaaaagag
     2581 atgcagaatt tctcttatac ttactttgtg aatacagtga aacgcgatcc agcttatgtg
     2641 aatctttcag aagagcagat cgaatctttg tataatggga catggatcat agctactggg
     2701 atcgcagctt tgatgtcttc aagcatcatc catccaactg atgaacagat tgaaaagatg
     2761 atccaagaca gtatagatgc tatcttgaaa ttcaaagaac caatcgaaat cgattgaata
     2821 tactcaaaag ttccacaaat cagtaggtaa caagagatgt gtcaaaataa ggaaaagttc
     2881 gtttttctta cagcgtatgc atttttaact cccgaagaac tcttccatga atgcaatgaa
     2941 taaaagagcc atacagattg aataaatctg gatggctctt ttattgcaag tatattaaat
     3001 ttttgcgact agttctttag cgaaagcttc taaattttcg atatcttctt cttcagcatt
     3061 cagatccact ttaacatttt cggctccttt tgatgcgcct gtttttgcaa aagcagcatc
     3121 gaaatcatcc accgatttgc agaattcatc gtaaaaagta tcaccagatc cgcagacacc
     3181 gtaaattttt cccaatagat ccaattcttg caagtcttca taaaagtcta cgatctcatc
     3241 aggcagatcg ccatcatcgt atgtataagt agctactacg caaatatctg cctcctcgaa
     3301 ttcatctgca tctacttggg tacattcgtt gatttcgact tcgatatcca gattttcaaa
     3361 tgcttctgca acaatatcgg cgatttcttc agtgtttcct gtcatgctag catatacgat
     3421 ttttgctaaa gccatgatgt gcctcctcgt ttataattat ttaacaatta tgaattatac
     3481 caaaacatca ttgaaaagaa aataaaacct agagacacct ggggaatgtc atttggctaa
     3541 atgtatgccg ctatgttaaa atgagaggga tttgtaaaaa ggagacgaaa atatgattac
     3601 tttaaaatca gcacgtgaaa ttgaaatgat ggcagaatct ggagctttat tagcagatgt
     3661 acataaaaat ctgcgtgatt ttataaaacc aggaatcaca agttgggata ttgaggtatt
     3721 tgtcagaaac tatatcgaga gtcatggcgg aattgctgcg caaattggct ttgaagggta
     3781 tgaatatgcg acttgtatca gtataaatga tgaaatttgt catggattcc ctcgtaaaaa
     3841 gcctttgaaa aacggtgatt tggtaaaagt cgatatgtgt atcgatttga aaggcggcat
     3901 ttctgattct tgttgggcct atgtagttgg agaatctaca cctgagatcg accgattgat
     3961 ggaagtaacg aagaaagctt tgtacatcgg tattgagcaa gcacaagtag ggaatcggat
     4021 tggtgacatc ggtcatgcta tccaaactta tgtagaaggc gaaaatctag cgatcgttcg
     4081 tgattttatt ggtcatggcg ttggtcctac gattcatgaa gaaccagcag ttcctcatta
     4141 cggagaagct ggaaaagggt tgcggttgaa agaaggcatg gttatcacga tcgaacctat
     4201 ggttaatacc ggaacttgga aaatgaaaat ggatcctaac ggctggactg cgtatacaag
     4261 agacggcgga ctttcttgcc aatatgaaca tacattggct attactaaag atggtccaag
     4321 aattttgaca tcacaaggag aagaaggcac ttattgatct atccagtcaa taggtacggg
     4381 agaggtacat gaaagtttgg aacaaaataa aacaaaatga aaatctgatg cgctttattg
     4441 agacgactca aaagcgcatc actgattcag aaatgagtac gacctctgtc gtcgttgctt
     4501 attatttact cttgtcttta tttccattga ttattgcttt tggtaatatt ttaccgtttc
     4561 tccacatcga tcaagagact gtactgacct atttacgaga agtgattcct gagaccgttt
     4621 accagtttat cggtcctgca atcaaagacc ttttgacaca aagttcagga ggcttgttat
     4681 ccgtttctgc attagcggct ttatggtctg caagtcaaag tatcaatgct ttgcaaattg
     4741 cgatgaataa agcgtatggc gtagaaaata gaaaaaattt tttgatcgtc cgtttcttct
     4801 cactaattgt cattgtcttg ttcatgattg ctattagtgg ggtaactatc gttcttgggt
     4861 tgggacagct gattttggat gctattcagc caatcgtaca aatacccgtg aactttatcg
     4921 atcagttcca aactttgaag tggccaatca cattggtagc tttatttgtc atcatgttct
     4981 tgatttattt aattgtccct aatgctcagt tgaagttgaa gacgatcatt ccgggcacga
     5041 tcttcgctac tgtcggctgg atgctgttat cacaagtgtt tagtatctat gctaggtatt
     5101 ttgcaacgag gatcagcggt tatcagatca taggaagttt tattgtactc atgctttggc
     5161 ttaattttgc tgctacgatc atcattttgg gcggaatcat caatgcagtt gttcaagaat
     5221 acgtgacggg tcatcaaatc aaaaaacgtc gagatccaag ccgaaaatgg ttcaataaat
     5281 tgaaaactaa attttctcat aaaaaaaagt agaaacaaga gttctta
//
LOCUS       DAZKNO010000160         5327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000160 DAZKNO010000000
VERSION     DAZKNO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.164"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACG8MY_002304"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACG8MY_002304"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0431469.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACG8MY_002305"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACG8MY_002305"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0431470.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACG8MY_002306"
     CDS             2591..3376
                     /locus_tag="ACG8MY_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0431471.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACG8MY_002307"
     CDS             3389..4240
                     /locus_tag="ACG8MY_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0431472.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACG8MY_002308"
     CDS             complement(4364..4816)
                     /locus_tag="ACG8MY_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431473.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1812 a          864 c         1090 g         1561 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttcc atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatg
//
LOCUS       DAZKNO010000161         5252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000161 DAZKNO010000000
VERSION     DAZKNO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.165"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..952
                     /locus_tag="ACG8MY_002309"
     CDS             77..952
                     /locus_tag="ACG8MY_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0431474.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            1056..1820
                     /locus_tag="ACG8MY_002310"
     CDS             1056..1820
                     /locus_tag="ACG8MY_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0431475.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            1807..3249
                     /locus_tag="ACG8MY_002311"
     CDS             1807..3249
                     /locus_tag="ACG8MY_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0431476.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            3260..4591
                     /locus_tag="ACG8MY_002312"
     CDS             3260..4591
                     /locus_tag="ACG8MY_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0431477.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            4604..5062
                     /locus_tag="ACG8MY_002313"
     CDS             4604..5062
                     /locus_tag="ACG8MY_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0431478.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         1782 a          760 c         1104 g         1606 t
ORIGIN      
        1 ttaggcacga cctaagaaag ggttctttct taggtcgttt tttgtcttta aaaattgttt
       61 ggagacaaaa atatgtatga aggagtctgc aagttgtttt gttttatcat atagaggagg
      121 tgaattaacg gtgtatgtga tgaaaaagct gaatcataat gctgtattga tcgatgacga
      181 tggaatagaa aaaattgcga tggggaaagg gattggcttt tctgctaaag tgagtaagcc
      241 atttgatgtt gaggatgctg acaaattatt tgtattagat tctaaagaaa agatcaaacg
      301 attcagtgag atagtctcgc agattccaat cgaatttatg gattttgcgg aagaagtgat
      361 tgaactgata acagctagga tctcctctcc attagattca aatatctata ttacattgac
      421 agatcatatt tattttgcta ttcaacgaaa aagagaagat agagatgtca cagcaattat
      481 gttgccagaa atgagattcc tttatccaaa tgaatatgat atagcagtag aagtggtcga
      541 gcgaatcaat catcgatatc aaaccgagtt agggtcaaat gaagtaggat tcatcactat
      601 gcatatcatt aatgctgagc taggagagcc taatagttta aagagcttaa aaatagtaga
      661 aatgacgacc acgattttgt cccatctaga aaaatcctat tcaaacgttt atgataaaga
      721 atcactgact tatcatcgct taatggttca tatcaaattc ctggttaaac gcttgattta
      781 tcaggaagaa acgccagtag aaaatattgg tttttttact gaaacattta aacatagcaa
      841 agcgtattgt acagcactaa agattaaaga tatcatcgaa cagcaatttc aacttgccat
      901 tcaagaaaat gaaatcatct atttggcaat tcatctagca agaatctatt aaaataaggg
      961 cgtaactatc tataggcgca accaaatgac acatagtaaa tggactatga ttatttggtt
     1021 gcgcctattt tagtagaaaa aagaaggaga atgaaatggg aaaagtgatt tttaattcgg
     1081 atgattttgg ctatagccat ggtgtgaatt atgggattat ggatgcttat cagcgaggaa
     1141 ttttgacatc aactacattg atggcaaata tgccgggttt cgagcatgct gtgaaactaa
     1201 gaaaggaaat gcctaggcta ggtgtaggag tccatttaac attgacgtgt ggcaaaccac
     1261 tcctcaaaac tgtcgatact cttatcgaaa gcggagagtt cagatcctta tcttattatc
     1321 agcatccatt taacattgac aaagatcaac tgtatcagga atggaatgct cagattcaaa
     1381 aaatttatca tgcggggatc ataccaactc atttagacag tcatcatcat actcatactt
     1441 tggatgaaaa tcaggaggta gttgttacat tagcaaaaaa atatgatttg ccggttcgga
     1501 gcaactttga acgaaaagat gaagttcgtc atgtcgatta ttttgagcct tgtttcgatg
     1561 tcgtaggaga aactgatgaa aaaaaacgac aaatcaatac gagtgtggaa gaatatttgg
     1621 caagtttact catgatattg agagaagata aaacgatcga agtcatgtgt cacgcagcgt
     1681 atttggatca ggaattgtta aatggttcaa gttttgtgta tcctcgtacc caccaagtcg
     1741 aatttttaat tcattcagca tttgctcaaa aagtgaaaaa tgatcctttg attgagttag
     1801 taacatatga ggatatttaa tagaggagga ataaaaatga atgttaaatc aaatgtgcag
     1861 aagcttggaa gagcgatgct acttccagtt gcagcgatgc cattagcggg attgatcatg
     1921 cgtttatcag ctgacgatat gctgaacatc ccagtaattg gtgcagctgg aaatgccgtt
     1981 tttggaaact tggatatctt atttgctata ggagttgcaa tcggttttgt caaaaataag
     2041 gacaaaggaa tacctgcact tactggtttt ttagcgattg ctaccttgaa aaaaggctta
     2101 gaaattatgg acccagaagt gaatatgggg atttttggtg gaattatttc cggtttagta
     2161 gcggcatgga cgtataatcg ctttaaagaa caaaaattac cagtagtttt ttcttatttt
     2221 gcgggcgaaa aattcccatt gactatggtg atgattctac aaacaatcgc agctgttttt
     2281 ttgggcttta tctggccgct catccaaatt gggattgata attttgcaaa actgctcgtt
     2341 gatatgggag cattaggtgt ggggatattt atgtttttga atcgtttgct gatccccttt
     2401 ggactgcacc atgtgttgaa tacgtatatt tattacgatt taggaagcta tacatctcct
     2461 agcaaggaag ttttcagagg agagatgaca cgctttatca acggagatcc tcaggctgga
     2521 ctgtttttat caggtttttt tgtagtaatg atgtttggta taccagctat atgtctagct
     2581 atctataaag cagcttataa agaaaataaa gagatggtca aaggaatcat gggaagcggt
     2641 gcaggaactt catttattgc aaatatcact gagcctgttg aatttagttt catgtttctt
     2701 tctcctatgt tgtacgtgat ccatgcactt tttgccggtt tagcagggat tgtctgttac
     2761 ttattcaata tacgaattgg ttttacattc ggagcatgta ttgttgatta tctcatcaat
     2821 tttcgaatcg ctaccaatgc tattttgatt cttccaatcg gaattttctt ttttgccttg
     2881 tattacgtta ctttttatta tttgatcaat aagagaaaca ttcagacatt aggacgtgaa
     2941 gctaaggcag agtttggcaa tgaagtaacc ttagaagaaa cagagttagg gttagcaagt
     3001 aaaaattatg aatatatggc aacaaaaatg ctacaagcgt ttggaggaaa agccaatatc
     3061 ctggatgtct atagttgtaa tacacgccta cgggtagagg tagtggatcc caccatggtc
     3121 gaggaacagc gaatcaagca attagggata agcgggatca ttaaaccaac tgaaaaaaat
     3181 tatcaaatta tcatcggctt agaagtgact tatgttatgg cagaattcaa taaattatta
     3241 gaagaataga aaggaaaaga tgatgatgaa aaaacagatt atcactatag ctggaggcgg
     3301 gagtacgtat acaccaggaa tcgttcaagc cattttgaac aatgaaaaac ggttgccatt
     3361 atctgaaata cgtttatatg atattgatga agaaagaaat atggatatgt acttgattgt
     3421 taaatttatg ctgaaaagaa aaggtttttc aaatattcgc attcgtgcaa cggatgatcc
     3481 aaagcttgct tttacaggat gcgattttgt tttctcacaa attcgtgttg gaggattgga
     3541 gatgcgagaa aaggacgaaa agattccact taaacatgga ttagtaggac aagaaacgtg
     3601 tggactcgga ggatttgctt atgggatgcg ctcaatcaaa ggtttattgg aaattgtcga
     3661 tcatatacaa gactatgccc ccaaagcatg gattttgaat tatacaaatc cagagtcgat
     3721 cgtttctgaa gctgttcgtc gtaaatatcc gaatgtaaaa atgattaatg cctgtgatat
     3781 gactatttca attgaagaaa cgattgcagt gaattacggt tatgatcgta agaattggat
     3841 cgttacgtat tatggtttaa accactttgg ctggtataca tcgatttatg ataaagaatt
     3901 gcaacgagaa attatgccag agattattga aaaactaata acaaaagaaa tgcaagtcgc
     3961 cgattttaat ataggagata aaacttggca aaaaaccttt caaatgatgt cagtcatcac
     4021 gaaaaatttt ccaagtaata tcccgaataa ttacttagag tattacttat atcctgatat
     4081 ggtcgttgag catactgaca aagagtatac acgtgctaat atggtcatgg atggacgaga
     4141 gaaaaatacc aaagaaatgg ctgataaaat acgccgcggt attgtggaag aagtattgaa
     4201 ctttaatttt ggggagcatg gacaatatat cgtcgatata gctatttcct tgttgaatga
     4261 tgatcgtcgt cgctttatgc taatcgttcc taatcaaggg gcgattccga atctacggtc
     4321 agatgcagtt gttgaaatac cggcatatgt tggtgcaact ggtgtggagc caattacact
     4381 aagaaaacca atcagtgatt ttcataaagg attgatggag gcacaagttg cagtagagaa
     4441 gttactagta gatgcttact ttgaaggttc ttatcaaaaa gctttacaag catttacatt
     4501 gaatcaaacc gtaccaaatg ccagagtcgc aaaaaaaata ctagatgaaa tgatcaaagt
     4561 aaacaaaaaa tattggccag cattaaagta gaggtgataa aaagtgcatt tctttaaaac
     4621 aagaaacatt ctaaaagctg tagctagtgg tcaaattatt ccattaacag cagtaaaaga
     4681 cgaggtcttt tcttcaaaaa tgataggagt tggatacgct ataacgaatc atgatggata
     4741 tatccatgcg ccagttaaag gaataatcga taatatattt cctacccgac atgccataac
     4801 gatcgaaagt gaatctggca gaaaaatact gatccatatg ggtttagata cagttgaact
     4861 cgaaggaagt ccgtttcaga tacgggttga aagtaaccag gaggtaaaag aagggacact
     4921 gttagctatt atagatttga agaagttgga gcaagcacaa aaagatacgg tgatcattgt
     4981 agtttctcca ggcatttttg aaggaaattt attaaaacaa aaccaagaag taaagattaa
     5041 agatagttgt tttgaatttt agtgtacgat gaaaaaagtt aaatagggtg atatttatgg
     5101 gtgaatttac aaaatttttt tgaaatgata tgtttcaatc actagattta atcattttga
     5161 tttttgttaa ttatcgaatt tttttgttgc tcctatgatt tagaaaactt attttcagga
     5221 ttcttaaaaa taaatttgat tatttaagag aa
//
LOCUS       DAZKNO010000162         5069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000162 DAZKNO010000000
VERSION     DAZKNO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.167"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     misc_binding    complement(32..259)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(621..1823)
                     /locus_tag="ACG8MY_002314"
     CDS             complement(621..1823)
                     /locus_tag="ACG8MY_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0431479.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(1883..2086)
                     /locus_tag="ACG8MY_002315"
     CDS             complement(1883..2086)
                     /locus_tag="ACG8MY_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0431480.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(2228..3295)
                     /locus_tag="ACG8MY_002316"
     CDS             complement(2228..3295)
                     /locus_tag="ACG8MY_002316"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0431481.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(3353..3460)
                     /locus_tag="ACG8MY_002317"
     CDS             complement(3353..3460)
                     /locus_tag="ACG8MY_002317"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431482.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            3623..4435
                     /locus_tag="ACG8MY_002318"
     CDS             3623..4435
                     /locus_tag="ACG8MY_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431483.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(4809..5000)
                     /locus_tag="ACG8MY_002319"
     CDS             complement(4809..5000)
                     /locus_tag="ACG8MY_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431484.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1536 a         1053 c          790 g         1690 t
ORIGIN      
        1 aaaaaaaaat ccttttacta gcaaatacta gtaaaaggac gattatactc gtggtaccac
       61 cttttttcag aaaaaacatt ctttctctca gcctgacaat aaaatcagtg gtcttggtaa
      121 cgaaagactg ttcgtttttg cctacctttc gacaaaaaat cctctaaggc cattttcgac
      181 agtcaacttc tagcttttta caccaaccaa agcctctctt gaaccatttt ctgtctactt
      241 tccttctcat cggatactat tagaataaaa ttagaatagc atgtttgaaa gaaaagtcaa
      301 tagggaataa gaacaaacaa aaactaattt gttattctta gtaatttgtt tgtggaatac
      361 aataaagctt ttctaatatg ataattgttc agtcgtcctt tcatcttaga tcacttcctt
      421 aaaatgatca tacccgtttc aaccacataa aaaaggactt aattttgaaa aatttcccat
      481 caaaactaag tcctttttat tatagttatg caaatttgct tattttctcg ctatttccga
      541 tacatcttga acaggtgcaa ttcgactgaa aacactggtg caaaagcatt tatttgtctt
      601 atggtagaac cgtggaaaac tcagactggt aataaattgt cattttttga aagaatagcg
      661 ccctcttgaa cattatcaat cgcacttacc acttgttgat ctgactttgc atataagtga
      721 ccgtaagttc ctaaagtgat ttgaatattc gcatggccca atcgttcttg aatagatagc
      781 ggttgaatat tctgctcaat tagaaatgca acatgagaat gtcgtagcgc gtgaagctta
      841 atcggtttga cacccgcttt tttagcagct tcttttaacc agttcgcaaa agaataaggt
      901 ggcgtaaatg ttccgtccaa ctgagcaaca tattcaaaat ttccaatacg tttttgcaag
      961 tcataccact gtctgagttt taaggaaagc ttcttcccca acccaatcgt tcgataagca
     1021 catttagttt tgggagtcga aaagtaccaa tcatcttttg tattataaat caatgtcttc
     1081 tcaatcgtta tcgtgttttt ctcaaaatct acattcgacc aagacaaagc ttcaagttct
     1141 ccaatccgaa tgccagtata aaagagcatt tcatacacaa tcttccgatg gtgggtttga
     1201 atcttatcat ccttcaattc attcatcact ttttgaaact cactgtgttc ccaaaactca
     1261 actttcttat tctcagaagg aagcgaagaa acatcttcaa cgggattttt agcaatcacc
     1321 ccctctttaa cagcccgatc caatacaatt tttaggtgcc ctcgaatccg attgatataa
     1381 gattttgata acgtttcgcc attaactttt ttccgcgaat tacgtgactc tgtctggctt
     1441 agttcaaata accaatcctc aatatcgtga cttgtaatat ctcgaacacg aaaatgatag
     1501 aaaaactgca tacgcttttc gaaaatgaat tgcgcattct catatgtttg acttttgacc
     1561 tgtctctcgt accattttac aaacctatcc ttgtaaaact cttcgaaagt catttgattg
     1621 gtagaacaca gttgattagg aattgtcttg ttaaaatcat gtgtttccat gtacttgtca
     1681 taagcaattt tggcatcttt ttgtgaagca taacctcgtt tagtcttctg aacctttctt
     1741 ccattttcat ctatacctag atacgctcgg aagaaccatt tttttgtctt tttatcttgg
     1801 taaatatttt taatttttgg catcgttcca ttcctccctg tacttgagaa aagctacttt
     1861 aatagtaccg cgcttgtact gtttattccg caacttgaat gtgaaaaaga tgttcaatta
     1921 ctcgggatgg caccacacca acttggcgat tattataaaa atcaatacct tcaatccttg
     1981 caagatatgc cttgctctct ttaatcattc gtgcagcttg actaggttta aagcccatct
     2041 ctatcaaaat tgcacgattt accatgaaat cacattttgt ttccattctc tcacctcctg
     2101 attctttact ccttttaaaa taatcctctt tataatctat tgtttctcat tcgatggttg
     2161 tgttatactc gtcttgtcga ggcggagtac gttgcacaac ttgttgtgta agcagtactt
     2221 tgctttttta gtagcttgtt ttctcatttg atatactttg taattttcgt tcataatact
     2281 caacctcctc ctgataatac tccgggtctg ccttcatatt ttggagaagt ttgatctgtc
     2341 ttggattcat ttctccttgt tcaataatca aaggcaacaa ggaagaatga aacgtttgat
     2401 cgacctgtgc aataaaaact aatgatggtg aaacaccatg aattaaccaa ctcatagttt
     2461 tctcaaaact ttgaggaatt gtatctaaag aaatggtgat tgcgccataa ttgctaataa
     2521 aggctgccca tttggcatca acttttgcac ctagatcatt tggatcacgg tcataaaaac
     2581 aaattgctcc attaagtaac tcaaagaaca gtttggttaa atcatgggtt tttgaaataa
     2641 tctttgccaa gctttgcgct ttctcttttc gataccgtag ttcaaagcga ttctttactt
     2701 ttgcctcttc taaaggaatt cctcgtcgct tccgttgttc atggtccttc tgataaaagc
     2761 aaaaacgaca ctgactcttc tttgagccta aatacaaggt ctcaccattt gattcattgt
     2821 ttatgatatc cgtaccgcca tgagattcac tcacttgaaa agtggtccac acatggccta
     2881 gtattacctt atgcttaagt tctggaatgc tgagactccc cacaaaatca tctaaagtaa
     2941 aatcaatccg tgtaaagttc ccctgataat ctaatacctt acgaaaaaac tgttgccaat
     3001 ttgtttttcg tgtttttagc catcttgcta agtgtttaca gccttgacct gaaaactcca
     3061 tcacagttcc tttaaccgca tcgtcaatgg aataccgaat cgtgattact tgattccaca
     3121 cgtattggcc aatatagccc tttggcgcat aatccagttc ctcaaaacgt tcaatgggca
     3181 tttcaataat ctcttcaatt agctttaaat gttgattcga gaaaaagtga atggttagat
     3241 aatcaatcaa aacacggtta actggaacga cctcttcttg caaactatcc aacacaagat
     3301 tcaatttttc ttcacttaat tgtctcgttc ctttttctac tttcgataat aactatctcg
     3361 aaatccccac tttttctgct aaatatcgtt gtgtccaccc cctttcttct cgagaaactc
     3421 gaatggcttc accgagatca tgttgattaa cttggttcat agacaaacct ccttctgcta
     3481 tttacttgtc aaagaaccta ttgaacttat tttaagtaca tttttattgt acgcctgtat
     3541 ttattttaag tcaatatgtt ttgacttttt ttaagtacac acgtatacta aatttaagaa
     3601 cttatcctga ggaggagttt tcatggagat agacaagcaa gcagtcggca atcgaattcg
     3661 gcagattcgc caagaattaa agttgagtat ggagaaattt ggcaaactaa ttggtgattt
     3721 accgcgaagt acagtcaata actgggaacg tgggatcaac cttccaaaaa ccgaaaccct
     3781 tcaccaaatc gcagagatag gtcatgtcac caatgaatat cttttgtatg gtgatcaaga
     3841 gaatcaatac attttagaaa tgctccaaaa aaaggcaggt aagcttgatc ctaagattga
     3901 aggtctcata gtagacgaaa tcaaacacgc tgaccttgtt agtgaaaaag aaatggatcg
     3961 aatgattgaa ttttttatta caaatctcat tccaccaact gaacaagacc agtttacttt
     4021 ccaatgtatc gatgaaaaaa agcagcttta tcttggttca actaattttg gtaaggaagc
     4081 ccagctctat ttgcaacatg ataatcaaaa tcatattctg cacatgatgc cctttacctt
     4141 ctccaacttt agtgtggatc gcatactcgt ctatttagca aatcaaaaat cctattccta
     4201 ttttgggaaa catttaccca aggaactagg tgagaaagcc atccttctat actcaattaa
     4261 tcaattgacc agcgatgtgc gaattgcccc gttggtctat tcagaagaaa ccgcttctta
     4321 tcagtacaca gaggacaatc aatatctact agaacagcaa tatctttatc taccattcgt
     4381 gatggaagtc gagaaagaac gcctgttaaa tgcagcgtat ccatcatcaa aatgaggtga
     4441 caaaattgat aagcacgtga caaagaaaat actgctagga cactccccaa tgggtagttg
     4501 tcacagtttc ctgttctttg ttagccccct gttagaaccg ggggctttat tcctgcagtc
     4561 cggctacgcc ttcctgcaat agacaaaact ccagatcggg taattcgctt gtggcgaaac
     4621 ccgacctgga gtttgtctct gtattttgaa ccctaaaaat taatcaaaca gactgatatt
     4681 ctcataatca gcgcgaaata agccattaag tacagttttc ctacctattt ttgataaccc
     4741 gctggttagt taatattcct tttccgagag agataaatga ttggttaaat ctatatacat
     4801 ctcaaccttt aaaaatgttt tcgaaatact tcatcagtac atgtcataca aattaccagt
     4861 gcattctcaa attctagttc tttaattgat ttcagttgag gagcagttac ttttggcgga
     4921 aatttccatt tttgtccaaa cctcttcccc cgatgaaatt tctttgttgc aattattgca
     4981 atgaaaaatt tttgtttcaa aaatatacat aattacttcc tccaataaaa actatctacc
     5041 tctcaaatta tttttctcta ataagaaaa
//
LOCUS       DAZKNO010000163         5018 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000163 DAZKNO010000000
VERSION     DAZKNO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.168"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..1236)
                     /gene="hflX"
                     /locus_tag="ACG8MY_002320"
     CDS             complement(7..1236)
                     /gene="hflX"
                     /locus_tag="ACG8MY_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0431485.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="ACG8MY_002321"
     CDS             complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="ACG8MY_002321"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0431486.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2159..2896)
                     /locus_tag="ACG8MY_002322"
     CDS             complement(2159..2896)
                     /locus_tag="ACG8MY_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0431487.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2987..3514)
                     /locus_tag="ACG8MY_002323"
     CDS             complement(2987..3514)
                     /locus_tag="ACG8MY_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431488.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3694..4287
                     /gene="clpP"
                     /locus_tag="ACG8MY_002324"
     CDS             3694..4287
                     /gene="clpP"
                     /locus_tag="ACG8MY_002324"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0431489.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4391..4876)
                     /locus_tag="ACG8MY_002325"
     CDS             complement(4391..4876)
                     /locus_tag="ACG8MY_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0431490.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1329 a         1123 c          862 g         1704 t
ORIGIN      
        1 aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc ctttaacttc
       61 atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc ttctcaactc
      121 gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca ttagttccat
      181 cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct gagtagaaat
      241 caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt ctgccttatt
      301 gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt ttaagacagt
      361 ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca aaagatccat
      421 attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag gcaggtcttg
      481 gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac gccatctctt
      541 agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg cttgtgtcaa
      601 tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa tctgaaaaat
      661 ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt ctttcaattc
      721 tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta actttgtttc
      781 acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc cttgccctac
      841 taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc cttctttcga
      901 acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta ccggaagacc
      961 aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa agatgatcaa
     1021 atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt taccgataat
     1081 cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt ctcctgatgc
     1141 ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct ggtttgcttc
     1201 ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc agccattttt
     1261 tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca aaccactgtg
     1321 catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc gaatgtagtt
     1381 tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg aaaaattctt
     1441 tatatccaat accttgactg gcctggactt ctccaagctc atacacctta cgcgcttctg
     1501 ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca taaagaatac
     1561 tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc tcttctttag
     1621 gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc actttgcgtt
     1681 gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac tgattccata
     1741 atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc tcaggattcg
     1801 cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag agacccgtac
     1861 caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat tgtcgacctg
     1921 aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca atcaaatgat
     1981 gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc agatttttat
     2041 agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg agttccacac
     2101 ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt tccatatttt
     2161 acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca gttattattc
     2221 catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc gtccatgcac
     2281 gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa gtattttttg
     2341 gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta tcagaagtac
     2401 tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc aaagaatcca
     2461 aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat gtttcggcta
     2521 atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc attagcatcc
     2581 ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga actttagctg
     2641 ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct tccaatgttt
     2701 tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg tgcataacga
     2761 tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca cctactcgta
     2821 cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt ccgcgatggg
     2881 catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt aagaaaaaaa
     2941 gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag ctccaaacat
     3001 catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga gctttttttt
     3061 cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt ccgcctttat
     3121 aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt cgatgggaga
     3181 tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt tgcgctgaaa
     3241 atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat gcatcaaagc
     3301 tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc tttttattta
     3361 attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta cgctgttctt
     3421 tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca gcacctgtca
     3481 gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt gttttgtttc
     3541 tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt ttttatttga
     3601 ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat tatggtacac
     3661 tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac agtcattgaa
     3721 caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa agaccgtatc
     3781 atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc ccaattgctt
     3841 ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc tcctggtggt
     3901 tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc agatgtccaa
     3961 acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc tggtacaaaa
     4021 ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct tggcggcgct
     4081 caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac acgcgaacgc
     4141 ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga aaaagataca
     4201 gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat cgatgaagtc
     4261 atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt aagctctctt
     4321 atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt tattttatgg
     4381 atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga cctatctcga
     4441 cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa ggacggatca
     4501 ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct tctgttacta
     4561 atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat atcgtggtcc
     4621 ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac tgatgtccta
     4681 ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg attgtaccat
     4741 cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt aaaacagctt
     4801 gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg gcatttgatg
     4861 ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact aatcctattg
     4921 aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa aacctgctca
     4981 ggccagatgt ctatttacag tatctggtaa ctgacgtt
//
LOCUS       DAZKNO010000164         4873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000164 DAZKNO010000000
VERSION     DAZKNO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.169"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..1318)
                     /gene="nagA"
                     /locus_tag="ACG8MY_002326"
     CDS             complement(170..1318)
                     /gene="nagA"
                     /locus_tag="ACG8MY_002326"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0431491.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1394..2230)
                     /locus_tag="ACG8MY_002327"
     CDS             complement(1394..2230)
                     /locus_tag="ACG8MY_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431492.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2414..3631)
                     /locus_tag="ACG8MY_002328"
     CDS             complement(2414..3631)
                     /locus_tag="ACG8MY_002328"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0431493.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3780..3941)
                     /locus_tag="ACG8MY_002329"
     CDS             complement(3780..3941)
                     /locus_tag="ACG8MY_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431494.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            4014..4097
                     /locus_tag="ACG8MY_002330"
     tRNA            4014..4097
                     /locus_tag="ACG8MY_002330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4048..4050,aa:Leu,seq:gag)
     gene            complement(4337..>4873)
                     /locus_tag="ACG8MY_002331"
     CDS             complement(4337..>4873)
                     /locus_tag="ACG8MY_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431495.1"
                     /translation="LTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRA
                     MIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLV
                     ILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQ
                     FGLTVLGICTFIITVINR"
BASE COUNT         1477 a         1010 c          781 g         1605 t
ORIGIN      
        1 ttgctaatac gtattaacat ttttaatttt atctttacat tgttacttat tgaacaatag
       61 gaaattaaag aaacttttga aacttatgta aagtgatttt tactagaaca caaaaaaaac
      121 cttaaaacca aacttaagaa gtttagtttt aaggttaact taaattatat tatgcgtgga
      181 aacgttccac gccatctaga taagttgctt ccaatgtcat atcagggttc aatacgataa
      241 agtctgcatc gcgtccttga gcgatttttc cacatgtatc atcgatttta cagctgattg
      301 ctggaatcag actagccatc atgactgctt gttctggtgt tgctaatccc cagtctacaa
      361 catttttaat tgcttctttc aatttcaata tacttccagc taagttgcca gattctagac
      421 gtgctgttcc ttcttttact actactggaa attcgcctaa attgtaattt ccatcaggca
      481 tcccaccggc catcatacag tctgtaatta atgcgacatg atcatgtcct gctttttcca
      541 ttaggattcc cgcagcattt ggatgtacat gatggccatc acaaatcaat tcagaaaaga
      601 catgttctaa agataataac gcacccacca tacctggttc gcgatgattc aatccacgca
      661 taccattgaa agcatggaca aatacacttg cgcctgcttc aacagctttt tgcgcttctt
      721 ctagtgtagc attactgtgt cctagagaaa caactactcc ttcttcagtc acttgtttga
      781 caaactcttc tacaccttga cgttccggtg ctaaagcaat ttttttgatt aaaccacctg
      841 aagcttcttg ccattcatgg aaagtatcca aatcaggatc cccgaaataa cttgggtttt
      901 gtgccccttt gtgttcttct gtgaagaaag gaccttcaaa atagattcct ttgattttag
      961 ctcctggtgc ttcctgatac attttgccga ttgtctcagc tacatctttt aaacgttctt
     1021 tgcttgacgt caatgttgtt ggtaaaaatg aagtcactcc acaagataac agaccttcag
     1081 acatcacttt gatgccttct gcatcattat ccattacatc atgattcata tagccgtgaa
     1141 tatgtgtatc taccaaacca ggagcaatcc atttgccgct ttggtcaatg atgttgactt
     1201 ctccctcagg tagttctttg gtgtattcac caaaaagtcc atcggtaatt tcaagatagc
     1261 ctgcatgttt gacacctgac gctaaaaaga atttgtcagc atagataaaa gttctcattc
     1321 tattctctcc ctttgttttt ctacctctaa agtactctta tttagagaag aagtcaagaa
     1381 tggtctacac catttacaaa ttttttgaaa tttgtttcac agcttgtgct aagccttcat
     1441 agaatgctgc tctttctggt tcttctttac gcttgtatat taagctttta agcacaaata
     1501 aatcattgag gtaatagtag ctattcttct cttgagccca atagattcct tgattcacct
     1561 gttctaacgc ttcgtcgatt tcattttgat ggaataagaa atttccataa ttataaaaaa
     1621 tttgcgaaaa ttcacttttt gatcgttcat cagctgattg atgcagcagt tggatgcttc
     1681 gactcaaata gtagcgtgct tcagccgttt ttcctgccac accatatatt tttccgatac
     1741 aactaatcag ttgtatttct gtgtcagaaa catggattct ttctttttgg ctagagtagc
     1801 ttaatgcttc tttcaaataa taaagggact gagagatatt ttgacgcaac gtgaattcgc
     1861 aaacaccgag ataataataa tagcgttgaa agtcagttgc ttgatacaga ttttcagaaa
     1921 tatcagaaga ttttagtact tgttctaact tccgataatc acgtttttgg aaacaactat
     1981 ccaacagttc aaataattta tcgtttgtcc gagtagtttt tcgagagaga ttcatcacat
     2041 gatcaatcgt tacccctagc cgttgacaaa tctgcatcat caccaataca ttcggcagtt
     2101 cctcattatt ttcgatccga cttaatacac tttgtgaaca aatatcatgc gaaagcattt
     2161 tttgcgtcat tttttgttct tttcgaatct ctttaatgat cgttccaaag gactcgatta
     2221 acgtttccat aagtatcctc cccaaaatat gcgtattcgc attctattcg tattttactt
     2281 taggaagtcg caaacttcaa gaaaagactt gcaactaaat aatatacttt agttatttaa
     2341 gaaaactcca ctagaaagag agcctcagtg tgtttgtctg tggccctcct actgaaaaat
     2401 ttggttttta aaatcatttt tggcgatagg ttgctaggaa ttctctcatt ttcttagctg
     2461 tttgtttcaa atcatccatt ttaggaagat aaacgattcg aaaatgatct ggctggttcc
     2521 aattaaatcc accaccatgt acaagtagaa tatgatgttc atgtaaaaag tccaaaacga
     2581 atttttcatc atccaaaata ttgaatcgct ttgtatcaat ttttgggaat atataaaaag
     2641 cagcttttgg ttttacggct gaaagaccag gaatatcatt gattgcatta tagatatatt
     2701 ctctttgttc atagatcctt ccgcctggca ggagcagctc atcgacactt tgataaccgc
     2761 ctagcgcggt ttggataatt tgctgagaca atacattgga acacaaacgc atggaggaca
     2821 acatattcag cccttctata tagcctttga cgtgtgactt atctccactt aggaccatcc
     2881 agcctactcg aaatcctgct acacgatgag atttggatag accgttcagc gtaacaacga
     2941 acaaatcagg cgctaatgta gcaatcggaa tatgttctag accatccatc actaagcgat
     3001 catagatttc gtcggaataa ataatcaggt tattttgtcg agctacttca acgatttctt
     3061 ctaataattc ttttggataa agtgccccag taggattatt aggattaatg ataacaatag
     3121 cttttgtccg actcgttatt tttgatttga tatcatcaat atcaggatac cattctgcct
     3181 cttcatcgca aatataatga acaggtgtgc ctccagctaa tgcaatagaa gccgtccaca
     3241 acggatagtc cggcattgga accagtactt catctccatt gtccagcaac ccctgcatac
     3301 acatcgtgat caattcgctc actccgttgc cggtgtagat atcattgatc gtcacattcg
     3361 gaaaatgttt cagttggcaa tattgctcaa ttgcttttct agcagaaaag atccccttgg
     3421 aatctgagta gccttctgag tcacgggcat tcatgatcaa atcacgaatg acttcgtttg
     3481 gtgcttcaaa tccaaaaggt gcgggatttc ccgtgttcaa ttttaaaata ctaatgccct
     3541 cttcttgcat ccgctccgct tcttctagaa cgggaccgcg aacatcgtaa ctgacacctt
     3601 ctaatttgtt ggatttttca aattctctca tcacttagcc aatccctcat ttcaaaaagt
     3661 tagaactttc caaatttttt gttatttact atttcaatat catactccat cctagaaaat
     3721 tttgccactt atttaggaaa aacgtaagta ttaaaaaaac acaaacaagc gttgagaact
     3781 taattgtcat gatagtcatt ccatagattc gctgcccaat ccattgcaat tccacaaatg
     3841 attcctagca taaaaccaag caaaaaacgt ccgaatgcaa gtccaatcaa tacacctaag
     3901 atcattccca gtcctgaaaa ttctccgtca tatttctcca tcttaataag tcctcccatt
     3961 tttgagcttg acaactatgt aaaacctcgc tatcattagt gtgttattat tacgcagatg
     4021 tgttggaatt ggcagacaag catgattgag ggtcatgtga gcgttagctc gtgtgggttc
     4081 gagtcccatc atctgcacta caactcgtga agttaatttt gcttcacgag tttttttgtt
     4141 tgaacgtttc tttatgccat tatatacata tcactgtatc ctttacaaaa agaacgtaca
     4201 atcatgtaaa cagcctgagg tgagtatatg aaggtcggaa acaagctact cgtagaaact
     4261 tgcaagtgtt aaaacaatta atcgaaaaac gagtaaaatc cctcacctaa tactaaagcg
     4321 agggattttt tgtatattat ctgtttatca ccgttattat aaaggtacaa ataccaagca
     4381 cggttaatcc aaattgcata aattcatttt tttgtacact catgcttttc attagctctt
     4441 taactgccat tagtactaag atggcgataa caacataaag tatatagtac gtccgcattc
     4501 cggaagccca tactgtggga gaaaatccca taattaacct tgaacaaaat cccattatca
     4561 tcaaaataac taaaaaaata gaagttttct tattgtcaaa acttaaataa attccaacaa
     4621 ttaaacatag taataatgcc aagactacta aagtagctat ccaagtacct ggataaaaaa
     4681 gtgacagttt cgttcctaac tctgtaaaga gattattcag gttactacta ctccaaatca
     4741 ttgccctttt atttccattg acgtaagtaa agctttgacc cattgtatta ccaaaataaa
     4801 taatcaggtt taagaaaaac ggtaatgcag tcaatatacg cacataatag ttttgacatt
     4861 tcctatatgt tag
//
LOCUS       DAZKNO010000165         4843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000165 DAZKNO010000000
VERSION     DAZKNO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.170"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(96..758)
                     /locus_tag="ACG8MY_002332"
     CDS             complement(96..758)
                     /locus_tag="ACG8MY_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0431496.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            942..2577
                     /locus_tag="ACG8MY_002333"
                     /pseudo
     CDS             942..2577
                     /locus_tag="ACG8MY_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            2568..3650
                     /gene="uxuA"
                     /locus_tag="ACG8MY_002334"
     CDS             2568..3650
                     /gene="uxuA"
                     /locus_tag="ACG8MY_002334"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0431497.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3758..>4843)
                     /locus_tag="ACG8MY_002335"
     CDS             complement(3758..>4843)
                     /locus_tag="ACG8MY_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0431498.1"
                     /translation="RKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNT
                     FFKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDLSVDIL
                     RPNLPICREQRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCT
                     VFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQ
                     FYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDL
                     IVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVI
                     MRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         1551 a          835 c          934 g         1523 t
ORIGIN      
        1 ttggagttgc aaagtcaatg gatattttgt tatttaataa agattactca aaaacgaagg
       61 ttaattataa aaatagaaga ggtcgcaaga aaatgctact gctggaaata ctcgggataa
      121 cgttgagtaa cagtttcctg ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat
      181 cgagcttctt ctggtttttt atcaactaaa gccttgagta aatcataatg ttgttccatg
      241 atcgtttccc atggaagccc ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg
      301 gtattatggt catctaacca gttccaaact tcttttcttc ctgcaatttc gaaacaagct
      361 tcatgaaata aattatctgc acggaaaaaa gcgcgttcgt catttatttt gactgctttt
      421 tcttgttctt ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa tttggaacaa
      481 cattcaatca tgatttgctc ttccaactgt tctcggacaa agcgagcatt ttctgcagaa
      541 acaagattaa ttttggaaac atacgtacca ctttgaggta tcgtatacac gagttcttgc
      601 tgacgtagtt gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc
      661 agtgcctttt cagatatttt ccttccaggt tctaagtcag catagataat ttgtttacga
      721 atcgcttgat aagcttgtga ttgcaagctg cttttcatct ttattcctcc aaaatcattt
      781 ctatgaacgt agtataacag aagaggtctg aaaaacaatc tggtatgtca cattaactag
      841 taaatttgta taaatagtaa aattttggtt gacatagcga tttttattgc ataaaattca
      901 cttgaataca aaaactaata tgttagttta ggaggggaaa aatgattgat ttaaacaact
      961 ggaggaactt tatccctgaa ttatctcaaa aagaagtaga aatacctcat tttgaccgtg
     1021 catcattaag agaaaatgga caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc
     1081 tttatcgagg atttcatgca gaaatcgctc aacgtttagc ggatctaggc gaattgtcca
     1141 ctggtgtcat tgtttgtgaa acattcgatg aagaaataat tgaaagggct taccaagatt
     1201 ttgatgattt gattcttgaa gtggtcatgc caagcagtgg agagttcgtc aaacgtatat
     1261 tagctgcgac agctgacagt ctgtattgtc atccagattg ttctgaacac tttgataaga
     1321 tgattcgaca ctttgaaaat cctagtttac aattagttac ttttgctatt actgaaaaag
     1381 gttatcgatt gaaagataca aataataaat atataggacc gattgcagtc gatataaaaa
     1441 atggtccaaa gaagccatca catacaatga gtattcttgc tgtgctactt tataaacgtt
     1501 ttttatctgg agcttggccg attgcacttg tctctacaga taatttttca caaaacggta
     1561 gtcaattacg tacttctttg ctaacgatcg caaaaggaag gttatgtaca aaatacattt
     1621 atccaatact tatcgaatga agaaattgtt gcgtttccat ggacgatgat tgatcgtatc
     1681 accccgaatc cttccttgtc agttagcaaa atgttggcaa aagagggctt ttcaaatatg
     1741 tcgattttcc aaaccgcaaa aggaaataat attgctccat ttgtcaatac agaggacatc
     1801 cattatttaa tcatggaaga tcattttccc aatggacgtc cgaaattcga aaaagctggg
     1861 gtattcttga ctgatcgtgc aacggtagat aaagcagata cgatgaaggt gacgacttgc
     1921 cttaatcctt tgcatacgtc tttagctatt atgggttgtt tactggggta taggagcata
     1981 gctgaggaga tgaaagatcc agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa
     2041 ggcctcccag ttgtggagtc tccaggtatc attgatccaa agatctttct ggaagaagta
     2101 attactgtgc gcttttctaa tccaaatatt cctaatatgc ctcagcgaat cgccacggat
     2161 acctctcaaa aggtggcgat tcgttatggt gagacgatta aaaaatatat ggaaatagaa
     2221 gttaaagacc cgcagacact tgtattcata ccattgactc tcgctgcttg gcttcgatac
     2281 cttattggca ttgatgatca aggaaaacca ttcatcccga gtccagaccc actattgaca
     2341 gaccttcagc ataagctttc cgatattcaa ctgggtacgc aagatacatc acatattcat
     2401 gagaaaatgg ctcctatttt gtcaaataaa gaaatttttg gtgtagatct ttaccaagca
     2461 gagcttggca gaaaaataga agaaatgttc atcaagatgg tagcaggacc aaatgccgta
     2521 cgaaaaacat tacaggatta cttacaagaa catggaggaa atttatcatg gaaatgactt
     2581 ttagatggta cggatcacag gaagaaaaga ttaccttaga tgaaatcaga cagattcctg
     2641 cggtcaaagg gattgtaggc acattgatgg atataccagt aggagaggta tggcctaggg
     2701 agaggatcaa agaactaaaa gaagaaattg agtctcgtgg gttaacacta aaagtaattg
     2761 aaagcgtcaa tgtgcatgat gccattaaaa ttggattacc ttcccgtgag cagtatatcg
     2821 aaaactataa gcagacaatc cgaaacttgg cagaatatgg aatagaagtc atctgctata
     2881 attttatgcc tgtatttgac tgggtgaaat ctgaccttga ctaccgctta gaagacggct
     2941 catccacgtt agcttttata tctgatgata ttccagatga tccaagagaa atcgtcgcgc
     3001 gcatcgaaca ttccagcaat gaatttggat tgccgggatg ggaacctgag cgcttagctc
     3061 ctatcaaatc cttgcttgaa gcctatgcat cagtagacga agaaaaactt cgagaaaatt
     3121 ttgcctattt tctccaatca atcatcccaa catgtgaaga ggtaggggtc aaaatggctg
     3181 tacatccaga tgatccgcca tactcgattt ttggattagc tcgaatcgta aagaatcgag
     3241 aagatttaga ttggttgtgt cgcatagtag acagccctta taacggaatc actctttgta
     3301 cagggagtat tgcagaagat ccagaaaatg atgtatatgg gattttagca gaattttgcc
     3361 aaagagatcg aatcccattt gcacatgttc gaaatattaa atttttagct aatcgagatt
     3421 tctatgaggc accacataaa tccgaatacg gttcattaga catgtaccgc atactaaaaa
     3481 caatgtatga caacggattt aacggataca tccgtcctga tcatggaaga atgatctgga
     3541 atgagacagg aagaccagga tacggcttat atgatcgagc attaggtgct gtttatctaa
     3601 atggattatg ggaagcaatc gaaaaaggaa acaaaagtaa agataattag tgaaaaaaac
     3661 tgtaatttta ttatgtaaat aagaaggatg ttcaaattct tgatacataa atccggaggt
     3721 tgttaataag cattcggatt tatgtatctt atttggtcta ggaagtaact tgattttctt
     3781 ttttcgattg atccatgagt gcttcttctt ttaattcaaa gatatattct gagatcctca
     3841 taatcacttt tttccagtct tctggtgtga tgattccttt taaaatatag atttttttga
     3901 tatgagaagg caattgtttc aaagggcagt cgctgataac gatgttgcag ttctccataa
     3961 aatcattttg tattttgatc gattcatttg aaaaaatttg ttctatctta acgatcagat
     4021 ctttactgat ataaaaatca cgcgtatagt tcacaccaat cacaattcgc ggctgttttt
     4081 tgtagcgatc atacaaatgt actaggtctt caaccatcca ttgcacattt tccttttcta
     4141 aaataactgg taacaagtgt tgattttgtt ctctgaaatc catataaaat tgttcaatgg
     4201 cggtttggac ttctttcaat acgctatgtt cttgataaag catgaaagta ggaaggggcg
     4261 tatttggttg attgaagtta agaaatccat tataaagttt atttcgtgcg aatgccaatg
     4321 agtaaagaat cttttctttt tcaggtataa acaaatcaaa tacagtacaa agggactcca
     4381 ccatttctct tgaataaaga acatgaggaa cgtttgcttt ttttaaaagt ttgtattgct
     4441 ccaagcttgt ttcttccgat tcgattttcg tgatagaaag tcgagctaaa atattcacta
     4501 agatgcgttg ttcgcgacaa attggcagat taggtcgcaa aatatctaca gagagatcaa
     4561 taaaaaagtc aatatattca tactctgtga attctttttg tacgtctttc acagaagttg
     4621 caggaaaatt ctctttacat atttttaata gaaaatataa tttatctatc ataccgctat
     4681 ttagtcggtc aagtacttct tttttaaaaa aagtattcat cataaaagcc actacttgcg
     4741 gatcttcatc ataaaattct gtagcagtac ttgaaaagat gttccaataa atatctaatg
     4801 ctgcatattg aatttgtacc tcttttccag aaaaagactt tct
//
LOCUS       DAZKNO010000166         4828 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000166 DAZKNO010000000
VERSION     DAZKNO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.171"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..1665)
                     /locus_tag="ACG8MY_002336"
     CDS             complement(55..1665)
                     /locus_tag="ACG8MY_002336"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0431499.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1886..2131)
                     /locus_tag="ACG8MY_002337"
     CDS             complement(1886..2131)
                     /locus_tag="ACG8MY_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431500.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2857..4197)
                     /gene="glnA"
                     /locus_tag="ACG8MY_002338"
     CDS             complement(2857..4197)
                     /gene="glnA"
                     /locus_tag="ACG8MY_002338"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0431501.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4225..4617)
                     /locus_tag="ACG8MY_002339"
     CDS             complement(4225..4617)
                     /locus_tag="ACG8MY_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0431502.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1413 a         1043 c          791 g         1581 t
ORIGIN      
        1 aaaattgctt atccccttct cttgctactt ttctctttct atcaagttca aaaattattt
       61 tgtttcgtga tctgctaatg atgctccgac aaatgctttg attaattttt gcgggcgatt
      121 tggacgagaa atcaattctg ggtggaattg gcatcccaca aagaattttt tctctggcag
      181 ctctacgatt tctactaaac ggttatctgg tgagacacct gaaaagacta gcccttgttc
      241 ttcaaacaat tgacgatatt tgttattgaa ctcatagcgg tgacggtgac gttcttgcac
      301 aacatcggct ccatcatatg cttcagccgt cttcgttcct tttttgattt tacatgggta
      361 taatccaagg cgtaaagttc cacccaaatt ctcgatgttt tcctgatcag ccattaaatc
      421 aataatattg tttgctgtat ctggattcgt ttcagctgat gacgcatctt ctaatccaac
      481 gacgttacgt gcaaattcga cacaagccat ctgcatacca agacaaatac ctaaaaacgg
      541 aacgtcattt tcgcgagcat aacgaatcgc ttcgattttc ccttctactc cgcgatcacc
      601 aaatccacca ggaaccaaga ttccatctgc gtctttcaaa cgttcttcta cattttctct
      661 cgttaattct tgtgctttga tccaatcgat ctcgatgtct gaattatagg cgaaaccaga
      721 atgtttcaat gcttcaacta ctgagatata tgcatctggt aactcgacat atttaccgac
      781 aagcgcaatc cgtacttttt tcttcaagtt caagacacgt tcttctaatt ctttccattc
      841 tgtcatgtct gcttctggtg tatctagttt taaatggtcg caaacaattt gatccattcc
      901 ttgtgcttgt aaaactaatg ggatggagta aagtgtatct acatctggcg actcgataac
      961 tgcttctggt tcgacatcac aaaattgtgc caatttgttt ttcacgcttt gagaaacagg
     1021 ttgctctgta cgtaccacca aaatattcgg ttggatacct aaaccacgta gctcttttac
     1081 actgtgctgc gttggtttag ttttcatttc tccagcagct ttcaagtaag gaatcaatgt
     1141 tgtatggata tacataacat tatctgtacc gacatctgct ttcatttgac gaagtgcttc
     1201 aaggaatggc aaagattcta tatcccctac tgttccccct acttctgtaa tgatcacatc
     1261 tgaatcagtc attttagctg ctcgcatgat tttttctttg atctcatttg taatatgagg
     1321 aataacttga acagttgctc ccaagtattc gcccttacgt tctttacgca aaacttcaga
     1381 atagattttt cctgtcgtca cgttggagta tttgttcaaa ttgatgtcaa taaaacgttc
     1441 gtagtggccc agatccaaat ctgtttcagc accatcatct gttacgaata cttctccgtg
     1501 ctgatatggg ctcatcgtcc ccggatccac gttgatgtat ggatcaaatt tttggatagt
     1561 tacttttaag ccgcggtttt tcaataagcg tcctaaagaa gctgcaacaa tcccttttcc
     1621 gattgatgaa acgacaccgc cggtcacaaa aatatatttt gtcataagtt caaaaactcc
     1681 tttttaattt atgtgcagga aaagtctcag acaataaaca aagctcccta ttcttcaata
     1741 agaataggga gccgaaattc tgtaaacttc tgaccctcta aaagggtgcc caaggtgtat
     1801 aatacaagct gactaaggga aagtcaagta ctcaaatgaa tactatccgc ttacaatttt
     1861 ttctttttcg atatcctttg agaaattatt tcttaagtcc tttatgagtg caaaaaaaat
     1921 ttaacccaca taataagctt gatataaaca tagctaacgt taaacctaag caaatcagaa
     1981 aatcgttact gaaaaatttt aagtgaaata atagataaaa aaataaaaca aacattatga
     2041 tagataatat cccacctaac aaagaactac tttttcctac ttgcagcaac agtttatata
     2101 gcttatcgta gaaacaatct ttttctttca cccttattta cctcctttac gtaaaaaacg
     2161 tagtccacag ttctcggatt tttggcatat cttttctatt aaataatatc aaaaacaaaa
     2221 caattccact aattttttca aagatgacct ttttttcaac aaaagtgcct tgtagggatt
     2281 cattgaaggt aacgaatcac ataagtaaaa aataaggaga tgcgacaaaa gtcgtgcctc
     2341 cttatttcta aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatatcca
     2401 tcaaacatga gaagctgttt ttgaaattat ttcttgtgat tcggagttaa atggtaggct
     2461 cacaacctct ggataaacgg tattcaaaag ccagtttctc ggtattaagc caaatttatg
     2521 caaaaatgta gaaagccatt ttcacaaaat ttttcttaat actcgaaact aaacgtcttt
     2581 ttcaaaacct cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata agttcagcca
     2641 attcaaaaac aagaaaagct gtttttgctt ggctattctt attacttgtt gcagggcagc
     2701 tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaaa
     2761 aaacggcaaa atatgaggaa ctatttttcc gttagtttct ctaatactca ggtcaaaacg
     2821 ctgatttcac agcctctaat tcacggtatt cctgttttaa taaagttcta gatattgttc
     2881 tctttcccac tcggaaacag tttgtctgaa tgccgcccat tcgagacgtt ttgcttcaac
     2941 aaaatttgag aagatatgtt gtcctaatgc gtcaatcatt acattatcct tacgcaattc
     3001 tttgatagca ttatggatag ttgaaggtaa gtcctggata tgtgcagctt ctctttcttc
     3061 ctcatccatt acataaatgt tacggtcaac agctgctggc ggtgtgatct gattacggat
     3121 accgtctagt cctgcttgca acaatactgc cattgccaag tatgggtttg cagatggatc
     3181 aactgaccgc aattccaaac gtgtggataa accgcgagat tctggtacac gtactaatgg
     3241 cgaacgatta cggccactcc atgcgacata aacaggcgct tcataacctg gaacgagacg
     3301 tttataagaa ttgactgttg gattgcaaac ggctgtgtag gcacgagcat gtttcagcaa
     3361 accacctaag aaatgataag ctgtttcact tagttccaat tcaccgtctt tatcatagaa
     3421 aacatttcct tcatcattga acaaagacat attgcaatgc ataccagaac cattgatccc
     3481 aaataacggt ttcggcatga aagtcgcgtg aagtccatgc ttgcgtgcaa tcgttttgac
     3541 aacgagcttg aaggtttgga tattatcaca agcatctacc acatctgcat atttaaagtc
     3601 aatttcatgc tgtcctggcg ctacttcatg atgtgaagct tccacttcaa agcctagact
     3661 ttctaattcc aacacgatgt cacggcggca attttcacct aaatctgttg gtgcaaaatc
     3721 aaaataacca cctttatcat tcaaggtcgt tgtgatccca ccgtcttcgt ctaatttaaa
     3781 taagaagaat tctggttcag gtcctaaatt gaaagaggta aaaccaaggt cgcgcatatc
     3841 tttcaatgct cgtttcaaat tcccgcgagg atcgccggca aatggtgtcc catccggatt
     3901 atagatatca caaattaaac gagcaacttt tccatgagca ctttcccacg ggaagatcat
     3961 ccaagtggag agatctggat acaaatacat atcgctttct tcaattcgaa caaatccttc
     4021 aatcgaagaa ccgtcaaaca tcattttatt ttctaatact ttgtcgatct ggctcactgg
     4081 aacttctaca tttttgatcg tacctaagat gtctgtgaac atcaatcgta aaaatcgtac
     4141 gttttcttcc tcaatgattc gtttgatttc ttcacctgtt atctgtttct ttaccatggt
     4201 atccctcttc cttttttaat ttattcagtc aaagaattgt ctttgcaatc cttgcgactg
     4261 ataatgacct gttttgctta acccaccttg cgccaaaatc tcatcacgaa aaatttgtcg
     4321 aacgtcatga tctgtcaacg gcttcttgtt ttttgcttct tgttttttcg cctgtgccat
     4381 ttcgtataca cgtttgatcc cagccatatt caatccctct gaaagatagt ctttaatctc
     4441 aagcaacata tcaatatcat tcagcgaata catacgtcgg tttccttcgc ttcgttccgg
     4501 atgaactaaa tcttgttctt cataatagcg aatttgccga gcagacaaat cagtcagctt
     4561 cataactgta ccaattggaa atacggacat agatcggcgc aattcctttt ctcccatcag
     4621 acgtcccctt tcgttttata agttggctaa agtgtaccaa ttaaaaaaaa tattgtcaac
     4681 tgcatatgtt aggttttcta acatgagatc tctatttttt ataaaataac aaaaaaataa
     4741 gaacacaaaa aaggtatcta gttgattttc gtaccagata ccttttattt tttattatac
     4801 tgtactatcc tttattagtg tttttatt
//
LOCUS       DAZKNO010000167         4821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000167 DAZKNO010000000
VERSION     DAZKNO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.172"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1019..1987
                     /locus_tag="ACG8MY_002340"
     CDS             1019..1987
                     /locus_tag="ACG8MY_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0431503.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            2004..2528
                     /locus_tag="ACG8MY_002341"
     CDS             2004..2528
                     /locus_tag="ACG8MY_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0431504.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2904..3185
                     /locus_tag="ACG8MY_002342"
     CDS             2904..3185
                     /locus_tag="ACG8MY_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431505.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3187..3603
                     /locus_tag="ACG8MY_002343"
     CDS             3187..3603
                     /locus_tag="ACG8MY_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431506.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4225..>4821
                     /locus_tag="ACG8MY_002344"
     CDS             4225..>4821
                     /locus_tag="ACG8MY_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphopain proregion domain-containing protein"
                     /protein_id="HFQ0431507.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFS"
BASE COUNT         1844 a          832 c          767 g         1378 t
ORIGIN      
        1 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaaac
       61 actttagtga gttttgtaga aagtaccttt aaaggtactt tcacattacg aacgatacgt
      121 ccgtaggacg tactgaaagg gtgggattta tgagcgaact aatgcaaaat ttggctagca
      181 aaaaaagtta aaaaatatat gctaataatc gagcgaaatg aacacttttt caagtgagcg
      241 aatccgccat gacaaagctg aagcagttta tttcagcttt gtcatggcgt gtgcctagac
      301 ttttaaaaaa gtcggcacac gagcaaatat ctttatcaag ctctaatttg cgttttaaga
      361 cgtttgaaac gtctaagtat ataaatatat aagagtaggt ttaagagtgc ttaaatcgaa
      421 ttttagaggg cattcagaca actttagggt tgttagggct tgccctgacc gcctgtaagg
      481 cgctttaaga acaagataag ggatttatac taagactgtc ttaaaacgtc tcaaacgaag
      541 gctcatagct tctcaaagag ttccttatga acatttttag caaaggagcc ggaagcgact
      601 gcgcagtata atgtgagagc agagcgatca caccttttta agcgactgta gggagtaaga
      661 ttcccagttg agaaaaatgc gcgaatctgt ttggagaaaa aagaagtggt tttttaggcg
      721 ggaaattttg tccccctctt ttataatctc tttttaaacc tctttttaaa cctctttacc
      781 gtcggctata tccttactct cccaagggaa tgacgctttt atctacatcg ttttgtctgt
      841 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttatct acatcgtttt
      901 gtctgtttgt acattgaatt agtacatcga cgatgttaat ataagatgta gataaacagg
      961 cattttgtat ttcataacgt gtatctcttg tgcgagtaag aaaggagcta ttttaaatat
     1021 gtctaatgaa atcgtaaagt atcatcatga attaaataca attccgttgc gaaaatttac
     1081 accagtcgaa atgaatttgt tcttttctat cgtatcaaga atgcgtgatg taggtgataa
     1141 aaaagtacaa tttacattcg agcaattaaa agatttaagc aattataaag caactgcaaa
     1201 tgttcgattc atagatgatt tagaaacaac gtatgacaaa ttaatggatt tacgttttgg
     1261 tagaagaagt gctgacggtt tgcaacgtga acgttttgtt ttatttaatc aatttaaaat
     1321 cgacggaaaa tctgatattc cttttgctga aatacaagtt catgaaaaag ccttaccctt
     1381 acttaataat cttgaagaat gggtgcgcta ttctctccaa caattcaatg aattagaaag
     1441 cagttattca aaaacaatgt ttcgattact aaaacaatac agaaccaaag gtttcgctta
     1501 tttttcaaaa gaagactttc atgaattact tgatattcct aaaagttaca accaaggtac
     1561 gattgatcaa cgtgtgttca atccaatacg acaagaacta accgcaatct ttaaaggttt
     1621 aacaatcaaa aagaaatacg gaaaaggacg gggaaagcct gtcattggct atcaatttac
     1681 atttaaacct gagataaaaa aatcagacga ctttaacaaa catgccccaa caaaagaaga
     1741 acaaaacaaa gcattagaac tagctaacca acaagttcaa tctgaaaaag cacctacttc
     1801 attacaagaa aaaatcaaag aacaaaagcg aaaattggaa cttttacaac agttagaaaa
     1861 gttagaaaga gaacaaaata cccaaaataa agaaaataat gcacaaaaat cagagataaa
     1921 ttccgatatt ccttcagaac taaaaaaaga cttattagac aatcttcaaa acttattcaa
     1981 agattgaggg gtttttagat tttatgacag aaaaaacaat caaagaactt gctgaagaat
     2041 taggagttag taaaacagca attaacaaaa aagtttctga cgaaaatcga aaactttggt
     2101 tttcgaaaat cggaaacaag tttgtgatta atgaaaccgg acaaaaggct ataaaatcaa
     2161 tgttttttaa cgaaaacgaa aactcaaatc aacaactagt tggcgaaaaa acgaaaaccg
     2221 aaaaccacaa aaacgccaac caagttcccg aattagaagt cctttctctc ctagaacaac
     2281 agttaaatca aaaagatatg caactacaag aaaaagacga gcaaataaaa caaatgcaaa
     2341 aattactcga ccaccaacaa caactcaccc tgcaagccaa ccaacaaatc gagaaactgc
     2401 aggaacaact acagttgact tatacggaag aatcttcgga aaataaatca acgactcttt
     2461 ctgaagaaac agaatcgata gaaaaacaat caaaggcgga gaaaaaatgg tggcaattct
     2521 ggcaataaaa ccagcaattt aaattaataa aaattgctgg ttttattctt ctttttgctt
     2581 ttgcgagcag agcaacatgg acccatgttg cgatattttg gtggcgcccc aatccccgtt
     2641 ttttcgttga atcttactcg gactgcactc gttttacagc tgatttttct gaattttgaa
     2701 agagcataag gaaacgtggt atcactttct tacgaaaggt gctagatagg ctgtgcctat
     2761 cacgtttctc agaaaaaaat ttgctatcac ttttttgcca ttgtggctag gctatgtata
     2821 gtgttttggc gaaaaatagc tatacaaata ctatatttgt atggtataca taaattatac
     2881 gctgatacga aaggaaaatt tccatggaca ttttagttcg aaatatcaat cccaaaatta
     2941 ttgcaagaat agataaacgt tgtcaggaac tcacaaccaa aacgggcaaa aaatggagtc
     3001 gaaataaata tctaaaagtt ctaatcgaaa gtgattttga ccgtcaactt atgaattaca
     3061 aaaaagatca attcgattta ttactcgaaa aatttattgc cattcaaaca gaaaatacga
     3121 acatactgaa tgactatgtt cacacaaaca accaactcat tgaaacactt atcggaggaa
     3181 tataacatga gaaaaacttc tgttcaaaaa cacgtaaccc tttatcccag tcacgttgac
     3241 gttttagatt ccatcatcga aaaaaatcca gacgtcaaca attattcaga agccattcgt
     3301 tttttatgta tgagttatga acctgatacc aatacaacca caaccagtaa ccaagcaaaa
     3361 ctcaatgcca tgggaaaaga aatttctatc atgacagaaa tcatggcaga atttgctgat
     3421 ttatatctaa aagatactgg tgttcttatc ggtacaaatt ctaaagtgta tcaagaagtg
     3481 aaagaaaaaa ttgaaaatca actcaatcct tcccgacgaa atcaacccaa aaatcgtcaa
     3541 aaagaaacca atcaaacctt tacttctgga aattttgacc caacaaactt tctaaaaaga
     3601 taaaaaaaga aaccgatccc taaaaaaatt cctcgatttc tttttaggga tcagtcttcc
     3661 agcaagattt gcaagttggt accaacttgc gaaaaaaaag aaaaatccgc ttatgcaact
     3721 tcttcttttt gtattttgag gcgctacccc aatccccaag tatcggttat ttaatagcgc
     3781 ggaaatccaa agaataggac gtaccctgaa acaaaaaaag actgctctca cgagcagccc
     3841 aaaatgagat tatcctattc atcaacataa aaatattaca aagtgaagaa ctagagaaag
     3901 acaaaaaaag catatcaaat cctctcaaaa ataacaagtc ttaaaaaaat gacagataaa
     3961 aatgcacaaa gaattagata tagataaccg cgaaaaatca agctttagaa agttgctttt
     4021 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     4081 ggcatagtgt acattatgtt aactaacgct ataaataacg attatgttcc caaaacaata
     4141 ttttatgaac tataacaaaa atttgttatc gcatattgta tattttaatt ataggtgtat
     4201 accacttaaa tttaacacaa aggcgtgatt attttgaaac ccaaaaagaa aatttatcta
     4261 ggtctcctct tttccttgat gattggaatt tttagtatag gaattacaaa tacctatgct
     4321 tcagaaaatt ctaatttcaa tatcaactcc caaccaattg aagaacaagc aattaatttt
     4381 gcgaaaactg attttgaaca ctccataact tcaattcttt taatggaaaa taacaccgaa
     4441 actgatatta actatactct tggttcgcca tttaaaatgc tcgatggaga aaatacggca
     4501 aatagttcta ttttctttcc agtattttca gacgctgata atacgattaa atatatttat
     4561 accgtcaaaa aaaatacaaa tggagaatat tcaggaagta tttcacaatt tttagcaaaa
     4621 gaacttcaaa atctagtaga tagtaatata acagatacaa ccttattttt tcaagataat
     4681 agcatttatt attccgataa tggacaaatt aaactacttt ggaaatcacc tttaagtaca
     4741 gaaaaaaatg gtgtttcttc ctttacagaa aaaaatgatc ttatatccgt aaatataaaa
     4801 aataaaatta aaaatttctc t
//
LOCUS       DAZKNO010000168         4769 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000168 DAZKNO010000000
VERSION     DAZKNO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.173"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8MY_002345"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="ACG8MY_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0431508.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="ACG8MY_002346"
     CDS             complement(2064..3062)
                     /locus_tag="ACG8MY_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0431509.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="ACG8MY_002347"
     CDS             complement(3288..3941)
                     /locus_tag="ACG8MY_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0431510.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8MY_002348"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="ACG8MY_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0431511.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1400 a         1003 c          743 g         1623 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaat
//
LOCUS       DAZKNO010000169         4733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000169 DAZKNO010000000
VERSION     DAZKNO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.174"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..694
                     /locus_tag="ACG8MY_002349"
     CDS             23..694
                     /locus_tag="ACG8MY_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0431512.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="ACG8MY_002350"
     CDS             753..1193
                     /locus_tag="ACG8MY_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431513.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="ACG8MY_002351"
     CDS             1205..1873
                     /locus_tag="ACG8MY_002351"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0431514.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="ACG8MY_002352"
     CDS             1937..2560
                     /locus_tag="ACG8MY_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0431515.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="ACG8MY_002353"
     CDS             2616..3002
                     /locus_tag="ACG8MY_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0431516.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3215..4606
                     /locus_tag="ACG8MY_002354"
     CDS             3215..4606
                     /locus_tag="ACG8MY_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0431517.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1575 a          782 c         1007 g         1369 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt ttttattact
     3061 aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa aagtcactga
     3121 aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta cttccttcga
     3181 tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca cagttttagt
     3241 ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc gccatacaaa
     3301 attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg gtctgcttca
     3361 attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga tcgggattgt
     3421 aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt ttatttcgat
     3481 cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt
     3541 attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg gttcaacaat
     3601 gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag cacgaatctc
     3661 tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc agattttatc
     3721 ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg
     3781 agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc
     3841 aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca ttatgcggat
     3901 cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga caaacgtttt
     3961 ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt
     4021 agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa aagtcaatcc
     4081 aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt cacgttcaag
     4141 tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag tagatgatgc
     4201 aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct gtgctggcgt
     4261 ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg tctttagttt
     4321 ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg tagctggagt
     4381 cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact tgccagtagc
     4441 aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta cagcagtaaa
     4501 cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag atttagatcg
     4561 cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg
     4621 tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag tttctcaaat
     4681 ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg aat
//
LOCUS       DAZKNO010000170         4678 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000170 DAZKNO010000000
VERSION     DAZKNO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.175"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..2378
                     /locus_tag="ACG8MY_002355"
     CDS             6..2378
                     /locus_tag="ACG8MY_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0431518.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIREEIERFEKGESSFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQSELQAGIQAVANNIGIQIRDITESEIYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKLSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVQQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2439..3899
                     /locus_tag="ACG8MY_002356"
     CDS             2439..3899
                     /locus_tag="ACG8MY_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0431519.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEAKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLITELKPKHERQNF
                     LVRDNRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVHCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFDQQLSVNDSIEAINLKD
                     ICREVAKELKGCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAILFQQGRQQERE"
     gene            3910..>4678
                     /locus_tag="ACG8MY_002357"
     CDS             3910..>4678
                     /locus_tag="ACG8MY_002357"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFQ0431520.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKTTRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYRYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTYKRLWINSLEKEAIYQGFQQLRDAGETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGISGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKAQVTAQNGTFDAKLDPNETQVTQEAFEA"
BASE COUNT         1785 a          778 c         1011 g         1104 t
ORIGIN      
        1 cggacatggc agaagaaaaa gaaccactag acaatagtcg tttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaatg aacaaatcga aaagatgttt gaacaccaaa
      121 aaatgacgaa cggtcaacca atgaacgaca agaggaatgg ccgttcatgg tttaaccagc
      181 aagaacgaat cgaaaataaa gtccaaagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aggtagaaaa acttgaaaga caagaagagc taaaggaaat gggttataac aagtacggcg
      301 gactagatat gacgatcgaa aatattccaa gaatcagaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gtctttttca gccgccacga tccgaaaata tcaacggaag ttggaaacat
      421 tagaacagct gaaagaaaaa tcagaaaaag gaaaagaaaa tatcctccct gaagtacaag
      481 cgatcattga cagcggacga gtaacgcaat ggaaaaaaaa tccgaccatt tattttctga
      541 aaggctatcg aaaagtcgct ttagaactga atgaaaacgg ggaatttgag gaatctaaaa
      601 aataccaagc cacatcggaa gaggaaaaag cagctgttcg tgaattactt gaagaggctg
      661 cgccaactga ttctctgaag gagaaagaaa acgatcattt ctatcgagta gaattcaacg
      721 aaaatgatcc agaaaaagga ttgaaaagtt atggtggaga gatcgtaaca ttagaattga
      781 tcgatcaact caaaacgttg gacaaccaat acgctgcaga agcaggttat tataaattct
      841 attttgaaga agtgaagaac ggagaagtag tcgatcagat ccgaatggat ctaggggatg
      901 gcgtcaacag taatcaaccc atctatttag agttagcaaa attgtgcgaa caagtacctg
      961 agaaggaaca aaaacaaacg gtcaaaaatg aacctgtttc gaaagaacaa atgcagtcga
     1021 aagaaacaaa aacaaaacaa aagggatatc aaaaacgaac ggcagaagag attaaacaag
     1081 aagttaaatc actttcgcaa caggcactgg atgcggtgaa aaagcatacc cattctcctg
     1141 aagacgtcaa agaacttttg aactttatgt ctcgttttcc agaacgatcc tttagaaatc
     1201 aattattgat tgaggaacaa tttccaggtg caacggcttg tttaggtcgt gccgcgttga
     1261 aaaaagaagg aatctatatc aaaaaagggg aacaagggaa caagatcttt gtcagaaaaa
     1321 cggtcaaagg attttatgag aaaggacgtg gctttgttcg agaaacagaa gctacaccag
     1381 aagataaaaa acgaatcgaa agtggtcaaa tcgaagtgat caaaaaacct tactacacga
     1441 ttgaaaaagt gtttgatatc acacaaactc agttaaaacc tgaagactat ccgaaaatct
     1501 tcccgaaccg tgtctttgat tttcaattgg atcaaacagg gcagtcagaa cttcaagctg
     1561 ggattcaagc ggtagccaac aatatcggga ttcaaattcg agatattacg gagagtgaaa
     1621 tctatcaaag agaattagga caagcacgtg gggcgtatgt tcgtaacgaa tttactggcg
     1681 aagaagaaat cgtcatgaat actcgaaata ctcctacgca gcatttagct acaagtattc
     1741 atgaattagc ccatgcaaga atgcacaaac tttcagaatt agataccgca actaaagaat
     1801 tgcaggcaga aatgacttct tatattgtgt gcaaacattt tggaatggac acgtcggaga
     1861 aagctattcc ttatattgca gcatggacga agaatggtca gacgttggat gataaggaag
     1921 ctgcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa ttcattcaaa
     1981 ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa ccagcagaag
     2041 tgcagcagga gcataaaaat actgtagaaa cggcagaaca aaagaatatt gtgcctgttg
     2101 gttcactttg gatcgacaag aaagacggga aagtaatgga agaagatacg ggtcgaacgt
     2161 tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagta attttaaaag
     2221 gaaaaagtta tggaaaagag atccaaacgg cgttaccttc agaagaattt gcgctaggaa
     2281 atccattccg attttcactt gagacagtgt cagaagtggc caaacgaaaa aatgaaatct
     2341 attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaagaa agactatctg
     2401 aaaaatggat agtctttttt cttttgggag gtttgaaaat gacagagaaa aaaatgagtc
     2461 tcattgatcg atgcaagcaa atggatattg tggattttgc acgaaacaat ggaatggctg
     2521 tagtcaataa aggacgtgac tatcgcttag aagaccatga ttcgtttgtt ttcgatcgaa
     2581 gaaaacaacg tttttattgg aatagtcaaa atattagtgg cgacattatt gaattagcta
     2641 aacttttttt tatagacaaa gagatccaag accctaagca acaatttaaa gcagccactg
     2701 attttattct aaagaatgaa gccaaaacag aacgagtaga aaatcttcat tttgagacag
     2761 aaaagtacaa agatcacccc gttgattatc aaccacttac agaaaaagga aggaattatc
     2821 tgaaggaaga acgaaaattg ccagagtggc tgattgatta tgcagaaaaa gaaggattga
     2881 ttactgaact aaagccaaaa catgaacggc aaaatttttt agttcgagac aatcgtttag
     2941 atcatgcagt tgccttttta tggaaagatc cacagacaaa agagactgtg ggggcgagtt
     3001 atcaaggaac gtttattgat tatgaacgtt ttggtgagag aggaacatat aagcatatag
     3061 ataaaaattc gacagcgaat catggcttta acctaaagat cggtgaccct aagcagttaa
     3121 aattttttga gagtagtatt gatttactta gctacgctgc actgaatcgt gatcagttaa
     3181 acgatacgtg gttagtctct atggaagggt taaaacacca tgtgatcagt cattattttg
     3241 gtgaggcagt atctgaattg agaaagaaac aagcctttcc tcaatcgatt gagatttgtg
     3301 tggataatga tcgtgcagga catattttct acgaaaagga acaattaatg ggcgcagtcg
     3361 atccatttac gaatcagaaa gtacattgtg aacgaggaat cgctaatgat tggcaagtgc
     3421 caaaagaata caaaatcatt tatgaagaag tcgcaaaaga agaaaaggtc acaccagaag
     3481 ctattatggc catacacaag acagaaaaca atctgcaatt aacgaaccaa ctggtttccg
     3541 ctcacaaagt caatgcgtct tttgaccagc agctttctgt taacgattca atagaagcta
     3601 ttaatttgaa agatatttgt agagaagtcg caaaagaatt aaaaggttgt gaacgcgtgg
     3661 atggtacgta cgattttgat cgtttttatc aagaaaaagg ggatattaat gctcagattc
     3721 ttttctctta taaagcagaa caatactaca aaggctataa aaatcatgaa catgaatttg
     3781 tacctgaagt gaaaaaggat tggaacgatc aattgaagca tgagatccag caacaagaaa
     3841 tcagaaagca aaaacgtgcc atattgtttc aacaaggcag acaacaagaa agggagtgaa
     3901 atcgtcatga tgaaaaccgt tattttagca gaaaaaccgt cacaagcgaa ggcttatgcc
     3961 gatagctttt ctaaaaccac ccgaaaagac gggtattttg agatccaaga tcgattgttt
     4021 tcgggagaaa ctgtgatcac gtatggtttt ggtcatttag ttgagttaga ttcgccagat
     4081 atgtacgatg aaaattggaa acaatggtca ttagagcatt tgcctatttt ccctacacat
     4141 tatcgctatc atgttcctaa agataagaaa aaacaattta acgtagtgaa acagcaactt
     4201 caatcggccg acaccattat tattgcgaca gatagcgatc gtgaagggga actgatagct
     4261 tggacgatca ttcaacaagc aggtgcagac cagggaaaaa cctataaaag actttggatc
     4321 aactccttag aaaaagaagc gatttatcag ggattccagc agttacgaga cgctggagag
     4381 acctatccaa aatttgagga agctcaagca cgtcagatcg ctgattggtt gatcggcatg
     4441 aacggtagtc cactatatag tttactcttg cagcaaaaag gcatatcagg aagcttctcc
     4501 ttaggcagag ttcagactcc aacgctttat atgatttatc agttgcaaga aaagattagg
     4561 aacttcaaga aagaacccta ctttgaagga aaagcccaag taaccgctca aaatggcaca
     4621 tttgatgcca agcttgatcc caatgaaacc caagtgacgc aagaagcatt tgaagcgt
//
LOCUS       DAZKNO010000171         4672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000171 DAZKNO010000000
VERSION     DAZKNO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.176"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..355)
                     /locus_tag="ACG8MY_002358"
     CDS             complement(65..355)
                     /locus_tag="ACG8MY_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0431521.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVDQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(491..670)
                     /locus_tag="ACG8MY_002359"
     CDS             complement(491..670)
                     /locus_tag="ACG8MY_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431522.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(667..1017)
                     /locus_tag="ACG8MY_002360"
     CDS             complement(667..1017)
                     /locus_tag="ACG8MY_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431523.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1010..2335)
                     /locus_tag="ACG8MY_002361"
     CDS             complement(1010..2335)
                     /locus_tag="ACG8MY_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0431524.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            2785..3399
                     /locus_tag="ACG8MY_002362"
     CDS             2785..3399
                     /locus_tag="ACG8MY_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0431525.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(3564..4568)
                     /locus_tag="ACG8MY_002363"
     CDS             complement(3564..4568)
                     /locus_tag="ACG8MY_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431526.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLE
                     LKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITA
                     CEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEK
                     RNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNK
                     SQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD
                     "
BASE COUNT         1463 a          864 c          698 g         1647 t
ORIGIN      
        1 aaaaaaacaa tattacgata tcatgatatt aagatatcgt aatattatac aattgtgatt
       61 gatattatat atgttttttt atttttttat catgtttaat cttgaaggaa tcatattctt
      121 tttctaaaga caggagcttt tcataaacat cagacatgtt taaactagag gccaggctta
      181 aatcataaac gaatgaagca ggtaatgatt ttattctacg ttgtcgggtt atccagcttg
      241 caaccgttcc ttgtgggtag gcatataact cacaaaattg gtctacagtg attcctaaat
      301 tcttgataat gtaaacattg atcgggtgcg agtaaacaaa tgtattgata gacatggtgc
      361 gtaagcctac tttcttttat tattgcgttt gcaataatag tttaacacac gcattattct
      421 agtatctact cctatttgta aaaacaaaaa aggattcata gagataactc tatagaatcc
      481 gtaaaatacc ttatatatat aacgggaaga tataaaacct aaaattatga taacctgctg
      541 cagcttctac atagaaagag aagcgctcaa caccaacctc atttatcaaa cgttggccat
      601 aaccccaaat ctcttctgat agctgatcaa aggttatata ctttagctca tattgctgga
      661 taatgttcat tctaatcgac tccatttatt atcctggtaa aattcgatat gacgtatttc
      721 gtcgtagtga attttgtctt ttccaacgta gatccccagc tcatcactac ctaagatctt
      781 gccaatgata tcttctggat agtatccttc agcgtttcgt tcttcttttt gaatagatac
      841 tgatctattt tttatgactg cagcgttcaa tacttccgca atctcttcag tcgtcatttg
      901 ttcttttgct aaattttgac tggttcgttg ttcattgtcg gcatcaattt cttcagtatg
      961 atcagataaa taaaaccctg cccactttaa acgtccacga tcattgtaat caatcatttt
     1021 taattccccc atttccgccc gcatgaccac caacaagtgt gcttctagta atgcttctag
     1081 cgccttctaa cattgaagaa gcatgtacaa tcgacgtaaa accatatttt tgccgaatct
     1141 tgtcaataat tttatctaaa ttttcttcgt caatttgctt ttgtggatca ctaaataaat
     1201 ccagctgaag cgaatcggtg tacacaagct tgctataggt tattccaaca tgccttactt
     1261 cttgtccttc ataatactta tcaaacaaga aaagacaata attctgcaat tcctttgtat
     1321 tatctgtgat agggatcttc atctggtgtg aaaaccctgg tctcgtttct aagatcgacg
     1381 tgccaatgtg taaatggaca caacctgttt tacagttatg ctgcctaatg cgtatcgcta
     1441 cttgttccgc catttctttt accaccagct cgatttcatt tcttctagta taatctcgtg
     1501 gtaacacttg tgaatttcca tatgattttt cctttgtcgg ttctggtggg attgctatat
     1561 ctgttcgatc gatcccatta gcatggaagt acagctgtaa gccaatcaca cctagtctat
     1621 tcttaatcgt ataaggattc cagttagcaa gatctctaat gctcataatc cccatctgat
     1681 tgagtctttt tttcattcga gagccaattc cccaaaagtc cgtcatttca ggaatattcc
     1741 atactttttc aggaacgttt tcgtacgtcc attccgcaac aaatccttca ttatgctttg
     1801 cttcgttgtc taaggctagt ttagccaaaa ggggattatc tccgacacct actgtagcaa
     1861 tcaatcctag ctcttcttta atattctcct ggatcatttg agccagcttc tttctccgtt
     1921 gacttcgtgt tccttcagtc gtaaaaagat tcagtgatcg ggtcactttt aagattgatt
     1981 catcgatcga gtaaatcagt agatcttcat cagccacata tcttctgaaa atattattta
     2041 cctgcatatt tcgcttgata tataggttca tacgtggtgg aacaacgtgt agtgttttag
     2101 gaaatggttg tggtaagtca cgtggtctac tcacatttgt aataccatac cgcttttttg
     2161 cttcaggaga agaagctaat atcaatcctg aaccagtatt atcagctcga ctcataacaa
     2221 caagttctgt tgttaatgga tctaaatttc tttctataca ttcgacactt gcgtaaaagc
     2281 ttttcatatc gattaaaaag taatcattta ctggctcttt tgaataatcc atcataaata
     2341 acactccatt ctttcacatt atacaaacat atgttcgata ttgtaaagaa gaaatagcta
     2401 aatacataaa aaaagaagca taggtatgct atcaccctgc ttcttctttt gatttgtatg
     2461 atcaatttat tctatcactt tactaattaa tttgaaaatc taataaaaac tctcactcta
     2521 aaagagtgag agactgcaaa ttagagtacg gacttttttg gagttccgtt ctttaatatt
     2581 aacaaatgaa taaaatcctt acaacatttg agaataagtt atcagtagac acttacaaaa
     2641 aattctcata ctatttcctc catcattagt ttttgtctat ataaggttta ccctaaatag
     2701 acactggtat aacattgaaa aatatggctt cctcgtgtat gattagggtg tattttaaat
     2761 agttgcagaa agtaggtaaa agttttgaga gttgcataca tacgtgtctc atcgatcgat
     2821 caaaatgaac aacgccagat tgaagaaatg aaaaaattcg gtgcagagag aattttcatc
     2881 gagaaacaat ctggcgcaac gattactcat cgacctgttt tccaagaagc attagattac
     2941 gtccgagatc aagatatttt tattgtggaa gctattgatc gacttggaag aaattatgac
     3001 gagatcatcg cttcagtcaa ttacttgaag aagaaaaatg tgaagctgat cattactagt
     3061 ttaccgatca tggctgaagc aattggcaat cctctcctgg ataaattcat taaggatctg
     3121 atcatccaaa ttttggccat gatcgctgaa caagaacgga ctgaatcgaa acgtcgccag
     3181 gcacaaggaa ttaagatcgc aaaagcaaac ggcgtctaca aagggcggcc caaattatac
     3241 agtgcagacg caaaagatcc ccaacgtcgc cttgtttaca aaagcatcgt tgaagatctt
     3301 aaaaatggtg ttgcgattgc taagattgct aaagactaca atgttacgag acaaaccgtg
     3361 tatagaataa aaaaagacag catggtcaat gacaaatagt tgatcatgct gcttttatta
     3421 tcttctcatc tttaatttcc tacatataat tactatctat aaaattaagt cttactcaga
     3481 acagataaag cataaaattc caacaaacca ttttttactt aactaaataa agagtctgtt
     3541 aatataatct tcacttattt acgttaatcc cctactgtat ttgttggttc cttatctata
     3601 ttttctacaa aatccttatt atcttgtgat ttagcttcgt ataattcttt gtatgtcgac
     3661 acattggtta tactaattcc tatagctagt tcttgagact ctacttttct ctctagctcc
     3721 tttgacaaag tgatttgaga tttatttggt tccgttggag tcaattctat agattcttca
     3781 taaaaatctc tatacataaa gctatcatta attttataaa aaaaattaaa tgtttttagc
     3841 actgccatat aacattcaaa acattcttct atagttggtt tacttccttg aggagaatga
     3901 accaatttat tacgtttttc tatgaaattt ttaatttcct ttcttatttc tttcggaaac
     3961 tcaatattaa agtacccttt aaacatgctc cctaataatt tgtctacagg gggagattgt
     4021 aaattctcaa atattagagt taaatcaggt ttctcttgtt gataaaattc ctttacccct
     4081 atttcacagg ctgtaattat cgaaataaac ctatctctag tatttgatat atttttactt
     4141 cttttaagga gtataagaga aaaggggata ctttcgtgtc catgcatgta attaactaat
     4201 tgttgagctt cagaagttat atctctaatt atatagttaa cggaattagg aagtgtcttt
     4261 aactctaatg aaatttctat ccaattacta tcttctaatc tgtatatgct cgaagtataa
     4321 tttgattcgt cagcaagcat cgctacatcc caaacactga tcataaaatc ttttaaacgt
     4381 tcaatcattt cagcaatatt gctaaaatca gctaattttt ctttagaata aaaaaaaaca
     4441 tttatatcag aatcaataaa ttctattttt tttattacta tatcgtcatc attatcttga
     4501 ccatttatgt ttattgttcc ctgagtgggg agtacttctt tcaaattcaa gttaaattta
     4561 tattccatta cttctcctcc aatatataaa aattacttaa ctgtatccaa atttaatatg
     4621 taaataaatc attaattttt agctaaattc tatgagaagt ttatttttgt tg
//
LOCUS       DAZKNO010000172         4637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000172 DAZKNO010000000
VERSION     DAZKNO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.177"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..1029)
                     /locus_tag="ACG8MY_002364"
     CDS             complement(67..1029)
                     /locus_tag="ACG8MY_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0431527.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGESI"
     gene            complement(1031..2470)
                     /locus_tag="ACG8MY_002365"
     CDS             complement(1031..2470)
                     /locus_tag="ACG8MY_002365"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0431528.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2655..4601
                     /locus_tag="ACG8MY_002366"
     CDS             2655..4601
                     /locus_tag="ACG8MY_002366"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0431529.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFVFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVYSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1464 a          791 c          773 g         1609 t
ORIGIN      
        1 tcaattggat atctacaaat tgtattttct aacggatttt atagacatta aaaaattaat
       61 ttgacgttaa atactttctc cagaaagtat tgagattggt aaaatataat ccttatttgt
      121 cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat
      181 tggttgtctt attgttgtca attgtgggaa aaatttctca atgaaagagg taccatcgta
      241 tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt
      301 gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt
      361 tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt
      421 tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt
      481 atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc
      541 aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct
      601 atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct
      661 tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt
      721 agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactcgat
      781 ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc
      841 aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag
      901 agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt
      961 aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt
     1021 tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt
     1081 gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat
     1141 taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat
     1201 caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc
     1261 gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac
     1321 tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat
     1381 aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa
     1441 tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac
     1501 tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc
     1561 aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa
     1621 atccataatc aagattttga atttctgaag cgcccacaag ttttactttc tctatgtcaa
     1681 gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat
     1741 ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac
     1801 actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg
     1861 tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt
     1921 gtgctccaac aatcgcatat aattgtcctt cataattaaa catttgtgga tcacgaaaat
     1981 gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt
     2041 ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt
     2101 ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc
     2161 cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta
     2221 aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat
     2281 tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca
     2341 atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct
     2401 taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag
     2461 gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc
     2521 cgttgaaaaa tcaatcattt aatttaaaaa aataaaatat atgacaaacg tttgacatat
     2581 ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa
     2641 aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa
     2701 aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg
     2761 acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaggc acatttaaaa
     2821 ttgatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac
     2881 ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta
     2941 aagataaaaa attcaacccc attatggcat taattaaatt attatcagat atttttgttc
     3001 caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag
     3061 ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt
     3121 cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta
     3181 tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca
     3241 tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt
     3301 cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta
     3361 tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat
     3421 tattatcagt aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat
     3481 tgttgtctaa tggaattaca gacattattg tttggctata taacactact ggatttattg
     3541 gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta catcaatcat
     3601 ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact
     3661 ttgtctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt
     3721 ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac
     3781 ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct
     3841 tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg
     3901 ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca acatctatcc
     3961 cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct
     4021 acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag
     4081 ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa
     4141 caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt
     4201 cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa
     4261 atggaaacac ggtctattct ccagtagatg gtactattca actcacgttt gaaactggac
     4321 atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata
     4381 ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa
     4441 aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata
     4501 caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa
     4561 atacagtcac tgagggtact gatttacttg aaatcaaata aatgtatttc cctgagtaaa
     4621 gatggaattt tctttcc
//
LOCUS       DAZKNO010000173         4590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000173 DAZKNO010000000
VERSION     DAZKNO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.178"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1137
                     /locus_tag="ACG8MY_002367"
     CDS             148..1137
                     /locus_tag="ACG8MY_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFQ0431530.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="ACG8MY_002368"
     CDS             1134..1553
                     /locus_tag="ACG8MY_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431531.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1562..1870
                     /locus_tag="ACG8MY_002369"
     CDS             1562..1870
                     /locus_tag="ACG8MY_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431532.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM
                     "
     gene            1874..2236
                     /locus_tag="ACG8MY_002370"
     CDS             1874..2236
                     /locus_tag="ACG8MY_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431533.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="ACG8MY_002371"
     CDS             2458..3864
                     /locus_tag="ACG8MY_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFQ0431534.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
     gene            4313..4432
                     /locus_tag="ACG8MY_002372"
                     /pseudo
     CDS             4313..4432
                     /locus_tag="ACG8MY_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT         1598 a          652 c         1042 g         1298 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagcttaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggccagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttca
     4021 aagggtgcgg ttttatgttt ttggatattt gcatagctaa agagaagtgc tattattgag
     4081 ttatcaaata aataaactga aaaaagagca aaaaaataaa gtcgagcaac gctggacacg
     4141 ttctcgacct ttacggaaac taatcacact agctccgtaa ctcaaattca ttatacgaat
     4201 aatttcaggt ttcttcaaga ctagagattg ctggttatga attttttaga gttacgtgaa
     4261 ctctggaaaa ttctttccac caatctcttt tttctttgag gaggaactaa atttgagtaa
     4321 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
     4381 tgtaaaaact ttttacaccc ccttaaaaat gcagtggtcg attgatcgaa ttttatgtct
     4441 ttgggttttg gggttttaaa acgaaaaaaa gggggcgatt ttcgccctct tttttgatgt
     4501 tttttcttat cttgatacta taggcaacta tttctgcgaa gaaatcgggg ctatagtatg
     4561 ggtctatccc ttgtggctct aaggaagaac
//
LOCUS       DAZKNO010000174         4527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000174 DAZKNO010000000
VERSION     DAZKNO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.179"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..461
                     /locus_tag="ACG8MY_002373"
     CDS             105..461
                     /locus_tag="ACG8MY_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0431535.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(702..1706)
                     /locus_tag="ACG8MY_002374"
     CDS             complement(702..1706)
                     /locus_tag="ACG8MY_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0431536.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            1872..2723
                     /locus_tag="ACG8MY_002375"
     CDS             1872..2723
                     /locus_tag="ACG8MY_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0431537.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            2740..2985
                     /locus_tag="ACG8MY_002376"
     CDS             2740..2985
                     /locus_tag="ACG8MY_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431538.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(3029..4474)
                     /locus_tag="ACG8MY_002377"
     CDS             complement(3029..4474)
                     /locus_tag="ACG8MY_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFQ0431539.1"
                     /translation="MLGVILMMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFD
                     FIYPIVESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYS
                     FEDPIPHFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANA
                     NKHKFTKEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESG
                     YYVKGEREKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRV
                     VADAGYKTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPL
                     LFSTITRDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTA
                     LNREIYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLW
                     KAKKPMAFYLKFLQRLTKRSWQLSHSFLSTV"
BASE COUNT         1443 a          850 c          791 g         1443 t
ORIGIN      
        1 caataagaac aaaatgagga aaagtttaaa attcagctgt tgccaacaac tttcaataga
       61 cgaaatcttc tatttcgtta ataataagga aagaggtgaa gtacatgaaa aaattaattg
      121 gcgtacttgt cgttttaggc atatttctcg gcggaagttt tgcagcttat cattattttt
      181 acggcggcga agcttattat acaaaaatca cgacgaatgg cgaaatgtca gctacccaag
      241 cagataacgg agagaaattc acgacttata cctacaagca agcggcttat aataaagatg
      301 gtgaaaaaaa acaggtgacg cttcgcgaag aaagagaaca tccattaaag atgaatgcgt
      361 atcttaaact caaagtcaat cctcgtaaag gtgtattaag ctgggaagaa ataaaggctt
      421 ctgaagtgcc taaaaatgcg gcagaaaaaa tcgataatta gtaaatggtc ttaaaatttt
      481 attagataaa aagaggctgg gacagaagcg tttaactcca agaaataaga aggagttgct
      541 tctgcttccg ccgtttatac gtgtttagag cttgagacaa aagtgttctt tacttttgtc
      601 tcacgctctt tttttgttat attcgtcttt tccgatcctt ctcttgacac aattcattta
      661 actgatcact ggtacttgaa cacctaatat tctagtcatt tttataaacg attcaatact
      721 tcatccacat ttttgatcat cacggatatc gtaccatccg gattattcac aaattcaatc
      781 aaatctgggt tgcgatatac atctactggt acgatcagtt cgatcccgtt tgatagcttg
      841 aatttttgtt ttccaaattt tttctcagaa atttcttgta cttcttttac aggaggtgct
      901 tctgggacaa atccttgctc ttgtacttct tctttaaaag ccatttttgc cgtgatgttt
      961 tccttgaaga ctttttccgc aatcatctca tgatccaacc gacctttttc ttctatcgta
     1021 tcatagacag cttctttcaa atcagccatg atatcaaatt cttctgtctc aaattttttt
     1081 ccaagatgct tggctacttt ttggatcact tttacatttt cttctaaaga aggtgcagga
     1141 acactttcga tcactcttcc agaaaaataa agctcttttt tgccagaaaa ctcatatttt
     1201 ttttcaatta attcgtaggt ccaatcagtc aaattcacag cgaatgcttc atctgctttt
     1261 tgtgttttcg cccctagaat tgctcgattc aaaatcaagc ggttgtcgat ccctgcttcg
     1321 tctgcttcaa caaagtgagt gtaagcctca cgataattga ctttcaataa agataagtac
     1381 atcactgtat ctaattcata caagacaaca aatacatctg cactaggtgc ttcttcactt
     1441 tcttggtaga tatcgaacca ccgctgaacg attgcttcac ttgcttccac aaaattttct
     1501 tgagcgattt taagtgattc aacgatcatg ctttcttctg ctagttgtcc agtttttgtt
     1561 tgagcagaag agattttttg gatcttcttt tgaagaaaat cacgaatata ttcttttgtc
     1621 agatccagct ctttttgtga aaagactgga gaaccagttt ctctgtcaat aatatgcaga
     1681 attgcttttt ttaaatagat atccattttc tcattccctt ctgtcttagt ttacggaaaa
     1741 aaaatcaaaa ataccagaat caaaatcatt ttcttttaaa caaaaatttc aagagtagaa
     1801 acaaaatgga ttcttttttt cgaaaaatgc gctacactta tagttgaacg aataaaaaag
     1861 gagctgaaag tatgaattca ttaacaagca cttatcgtct atcaaatggt tacgaaatcc
     1921 cggtagtagg attcggcaca tggcaaacac ctgatgggga tgtcgctgtt tcctctgtta
     1981 aagaagcgct ggctgcaggt tatcgccata tcgatactgc ccaaggatac aagaatgaag
     2041 aaagcgtcgg ccaagcgatt aaggaaagtg gaattcctag agaagagata ttcttgacaa
     2101 caaaattatg gaatgcaaac cacagctatg aacttgtgat gtcttctttt gaagaatcat
     2161 tgaagaagct ccaaacagat tatttagatc tgtttttgat ccactggcca aaccctgttg
     2221 cttttcgtga taattgggaa caagcaaatg cagatacatg gcgagcattt gaagaactgt
     2281 atgaagcagg taaaatcaaa gcaatcggtg tgagcaattt cttaccccac catctagata
     2341 cactagcaaa aacagcaaaa attatgccga tggtcaatca agttttcctt gcaccaggcg
     2401 aattacagac aactgtagtt gagtatgcaa aaaaacatga tatgatcctc gaagcatata
     2461 gtccattagg cactggtaaa atcttcgatg tgcctgaaat gaaacaaatc gctgaagcac
     2521 atgacaagac gattgcacaa gttgctttgc gttggtcgct gcaacacgaa ttccttcctc
     2581 ttcctaagtc agtgacacca agcagaatca aagaaaatac tgaattattt gattttgaat
     2641 taacagaaga agaaatgaaa caaatcgatc agttagatgg tgtagtcggc aaagcaaaag
     2701 acccagatac tacccaattt taagtcgaaa ggtgatttta tggatccaat cgaacgcttg
     2761 aattctctct cggaagaagt tacccagact tttcattccg attttgtctt tttgatcgat
     2821 gcagagaaaa tccaacattt tcctgcaaga aactggacac atgatcaaat catcgaagaa
     2881 ctgaaaaaac ggttcgatca ttcgctaatg gtcaaaccct ggcacgaaca tgaagtcatt
     2941 tattcacctg aactacctgt ttttgccttg ataccgaaaa aatagaaaga atggaacgga
     3001 ttatgtatat atacataatc cgttccattc agactgtaga caaaaaggag tggcttaatt
     3061 gccaactcct ttttgttagt ctttgtagaa attttaaata aaaagccata ggcttttttg
     3121 ctttccatag ccatgtggct agttttttta aattcatggc agcaaacgta agcatcgtgt
     3181 gcgtggcgac tttttcgaga cctcgatatt tcgtccaacg cataccatgc ttttcttttg
     3241 catctgcaaa tattcgttca atggtttgtt ttctcttttt ataaatttct ctattcaacg
     3301 ccgtatggcg ttggtgttca agttcatcca taagatccgc ccaaacatgt cgagttacta
     3361 ttttttgatg ttttgtactc aaagtacagt ttttaagtaa tggacaactc ctacagaatt
     3421 ttggatttga tttataggtt cgataaccat cacgagtgat agttgaaaat aacaacggtt
     3481 gattattggg acataaatag cagtcaaaat aggcatcata aacaaagtct tttttacgaa
     3541 acattccttt tactccatga ggtacagtat atggaagaat cgggtagagt ttcaagtgtg
     3601 acagaaaatg aacaaattta ggcgtcttat atcctgcatc agcgacaaca cgctttatat
     3661 ttggaaaagc tcttttcgac tgtttgacca gatcgattgc gacttggctg tcatgtacat
     3721 ttcctggtgt gattaatgta gataagataa aaccattatc ttcacaggaa gtatgcgcgg
     3781 aataagcaaa ttgtttttct cgttcacctt taacatagta accactttct ggatctgaat
     3841 gacttatttt ttgaagactc aactcacttt ctgtgtccca tgtaaaagga cgttttccta
     3901 cagcaatacg ttctttattt atttcatctt ctaattcatc ttgataagct ttagcttgac
     3961 cgtaggtcat ttcttttgtg aacttatgtt tattggcatt tgccttaata tgagtagaat
     4021 caatataaag gttgtcttcc gtaacgaagc cagcttttac agcttgttct agaatatgag
     4081 agaaaatgtc ttcaaataaa gttgtttctc tgaatcttcg aacatagttt ttaccaaaag
     4141 tagagaaatg cgggatagga tcctcgaatg aataacctaa aaaccaacga taagcaacat
     4201 ttgtttcaac gtctttaata gtctgtctca tcgaacggat accaaataaa tattggataa
     4261 atactaattt aattaagacc actggatcaa tactgggacg accatgggtc gaatacgtag
     4321 attctacaat gggataaatg aaatcaaact gaatcgcagc atcaatcttt cgaactaagt
     4381 gatctttagg tacaagatca tcaattgtta acatagcaaa ttgatttcta ccttcaatcg
     4441 attgttttct catcattaga atcactccca gaatagttta tacaatgagt ataaaacaaa
     4501 aaaactgcaa acaacactcc caattta
//
LOCUS       DAZKNO010000175         4425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000175 DAZKNO010000000
VERSION     DAZKNO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.180"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..1366)
                     /locus_tag="ACG8MY_002378"
     CDS             complement(47..1366)
                     /locus_tag="ACG8MY_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0431540.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1363..2016)
                     /locus_tag="ACG8MY_002379"
     CDS             complement(1363..2016)
                     /locus_tag="ACG8MY_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0431541.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2304..2824
                     /locus_tag="ACG8MY_002380"
                     /pseudo
     CDS             2304..2824
                     /locus_tag="ACG8MY_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2822..3541)
                     /locus_tag="ACG8MY_002381"
                     /pseudo
     CDS             complement(2822..3541)
                     /locus_tag="ACG8MY_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(3492..3701)
                     /locus_tag="ACG8MY_002382"
     CDS             complement(3492..3701)
                     /locus_tag="ACG8MY_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431542.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(3683..3751)
                     /locus_tag="ACG8MY_002383"
                     /pseudo
     CDS             complement(3683..3751)
                     /locus_tag="ACG8MY_002383"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            3978..>4425
                     /locus_tag="ACG8MY_002384"
     CDS             3978..>4425
                     /locus_tag="ACG8MY_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431543.1"
                     /translation="MPDLHLIKLLLGIKDGNIQITHVEKKKIHSINSIVVSATLIKTI
                     QPCPQCKQVNQHGLIVKNGKKKSLVLLNKCANQRTYLALAKQRYLCRGCHTYFTANTY
                     IVEKNCFIAKQVHYKILEELTEKQAMTTISKHCGVSWSTVSRTLASL"
BASE COUNT         1432 a          849 c          582 g         1562 t
ORIGIN      
        1 taataagtct ctatttttca ataactattg caaaattata tcttaattaa gaaaatattt
       61 ttattttaaa gataaaaaat tcttcatcct gcaatacttt atattctata ttgtaattat
      121 tcagaatatt acttacaata tataaaccta atccattact attttcctta tttaaatcaa
      181 atttaacatc aaatatttta tccatattcg aaattttatt gttaccgtat gaattctcta
      241 tatataacca atcattaact atcccaatat taattacccc atttacatca gtatatttta
      301 ccgcattact aatcaaatta gatagaataa tctttaaagc tgtttttcct atataaacat
      361 tctcgtctaa tatataatta ttgacagtta ttttcttttg atttgctaat atttcatact
      421 tttttaatat gtcttctaat gtatcattta ttttcaaata ttcttcatca ttattcaaat
      481 tttctataga atgaactgat aatatttgag aaatattttt tgttaaacta tcaacgattt
      541 caatacattc attaatataa atatctcttt ctttatattt tccaatattg tatttcatgt
      601 tttccagtat tattttaaga ctagcaagag gggttttaag ttcatgggat gcacctttaa
      661 aaaagtcata ttttaacttt tctaatttta aaatttcttt atttttaaat tccaaatcgt
      721 caattgttct taataaagta gaatacaaat cattaatttg ttgttttaat tctccaactt
      781 catcattaga gctaactttt aaactcattt ttttatcaag tttcatcatt ttatcagtaa
      841 caatttttat ttcttgtata ttatttttta ttgattttgc ataaattaaa gaaataatag
      901 cagaaaataa aattgatatt aataaagaat atggtaaaaa tttaaggctt aaatctttcg
      961 catctttttg catatcagct gtagaaacaa attgtagatt tgttttttta ccatcattaa
     1021 gctttatttc tctttcttca attatcaaag aattactatc actttctaaa ttaacattta
     1081 tattatcttt gatttgtatt tcattcttat tatttttttc ttttatgaat acttttattt
     1141 cactactctt ggaataaaac tccaaagttt gttctatata ttttatttct tttccattca
     1201 tattactaga aatttcattt gccatattat gaatcttttc ttttctcgtc tccaaatatg
     1261 ttttaggaaa gataaaaaaa attaatgaat gaactaatat aattattatt ccaagaacag
     1321 aaaatatttg tatgaacatt tttggaaata tctttaattt tttcattttc tctccaattt
     1381 atatcctaca tttcttatag tagttataca gtctaattgt aatttttttc taagttcttt
     1441 tatatataca tctattactc gatcataagg agtttcttcg ctatctttcc atacataatc
     1501 aataatttgc attcttgtta acacttgtcc attattatcc aataaacatt ttaatacttc
     1561 tagttctttt gcatttacat ctatttcttc accatttatt ttagctgtat agctattaaa
     1621 attaaccgat agatccttat attcaaactt ctctaaatgt ccataattct tcttaattaa
     1681 agaatctatt ctagctttta agaccggcaa tgaaaatggc ttttctacat atccgtctac
     1741 taaattagta aatgcatcaa ttttatattc ttcatcacta aatgcagtca agattagaat
     1801 tggtaaattg cttttctttc taatttcttt caacacttct aaaccattta taaaaggtat
     1861 ctgaatgtct aagataacca aatttatatc gctattaaat tttgacaagg cttctcttcc
     1921 atctttagct tgaataacag tatatccaaa ttctgaaaga tattcactta ttccctctct
     1981 tatcatatta tcatcttcaa cagttaatat tttcatatat acctccactt aagcctatac
     2041 actagatatt atacagcata ttatggctct ataatatcta gtgttggtga cgccttctgt
     2101 tgccaatact ttttcttttt tttaataaat gcaaacagac accttgaaat cctttagaaa
     2161 aaaggcttct ataatatttt gtgttgttgg aaaatcgaag gcgattttct actaacactt
     2221 tttcttcgtt tcaactcaaa aagagactca cttttggtaa aatttagtcg accaaaacat
     2281 caccctacca aaggagtcgc tctatggata atcatactag aaaattactc ggattaacag
     2341 ataaacattt attttttgat gaagaatggc tgattgaaaa agaatataaa ggcgttcaag
     2401 cgcagtttat taaagggaga ttggatgact caccttccca ttgtgaacac tgtggatcga
     2461 ttcgtattat ccgcaacggt tcctatacga cacgtactca gatgttgaaa gtcaaagaaa
     2521 agttgactat tttagaatta aaacgtaccc gttttctatg ctatgattgt gggcagacct
     2581 tttctgcgaa aacggactta gttgatgaac atcatcaact aacaaaggaa ctgaagcagg
     2641 cgattctcat agaactgtac gaaaatcaat cacgtaatct gaaaatcaga agaagtaccg
     2701 tcggctcaag cgatattgga aactcttact caaagattcc acgacattgg agccgctcaa
     2761 acggcattat catcgactgt ttaaacgacc aattagtcaa acagaaatcg tcgatgaacc
     2821 gttgtgcaca atggtgtcct gaacgtacag cgacaccttc catatccaga gcagtcgcca
     2881 catcacgggg atggatgcca tccaaattaa aagcaaccac gccagtatga tgttcaggat
     2941 cttgcggtcc gtaaatggtg ataccgccga tcgcttgtaa tttaggtaaa acgtaggcaa
     3001 ccagttcttg ctcataacga tgaatattct ccataccaat ctcattcaag taatcgattg
     3061 ctcctgcaat gttcggagta ccagcttcaa atttccatgg cagctctttc catgaactat
     3121 cgtaaagatt gacaaaatct atcatttcac cgccgaattc gactggctcc atttgttcta
     3181 acaattgacg tttgccatat aatacaccga ttcctgtcgg cccgcacatt ttatgcccgc
     3241 taaaggcgaa aaaatctgca tccaacgctt gtacatcaac tgtcatatgc ggtgctgctt
     3301 gggcaccgac tggactatcc tcaactacaa cagcctgttc aaccctcagc cgtttcaagc
     3361 acaacaaata accttctgga tctggtttgc cgacctgaag atcatcgatc gtcacaaagg
     3421 aatcaatttt ttccttaatc atcggatgtg cttcaaaaaa atagtctagc tcccgcagtt
     3481 cagaattgag actactccga ttttccagtg ctgctgatcc gctgctcaaa atgttcagca
     3541 tccactggtc tcccaaccgc ttgacttatc ccttcataaa aggacagttc tgctgccgtc
     3601 ccaacacctt gttgatagtc tgcaaaagga attttatagc cgaactgctc cagtaaatag
     3661 gcttcagtaa atagtgatga atactctcgt aaaacatcca ttcactgtca atcatcgtcc
     3721 catcaaaatc aaaaataagt gctttggtca tcttctttcc cccttaccca aaacaagcaa
     3781 aagctgtgtc ctgctctctc tacagtatca gcataaaaac tgccgtctga aaagtcaaag
     3841 gtgaaagaaa tgtggcgcta taatttctag gactgatagt aaatcattct atcagtcctt
     3901 ttgagcataa aaaaacaaaa catacagaag ctccgttaga attgaatcac cacaacacaa
     3961 tcaaaggagc gttctgtatg cctgatcttc atcttatcaa actattacta ggaatcaaag
     4021 atggaaacat tcaaatcact catgtcgaga agaaaaaaat acattcaatc aacagtatcg
     4081 ttgtttctgc cacgctcatt aaaacgattc agccctgtcc tcagtgtaaa caggtgaatc
     4141 aacatgggct gatcgttaag aacggaaaga aaaaatcgct ggtccttctc aataagtgtg
     4201 cgaatcaacg gacttatctt gctttggcaa agcaacggta cctctgtcgt ggctgtcaca
     4261 cctattttac ggcgaatacc tacattgttg aaaaaaactg ttttatcgcc aaacaagtac
     4321 attataaaat attggaggaa ttgactgaaa aacaagcaat gacgacgatt tctaaacatt
     4381 gcggcgtttc ttggtcaacc gtttcgcgaa cactcgcttc tctgc
//
LOCUS       DAZKNO010000176         4383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000176 DAZKNO010000000
VERSION     DAZKNO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.181"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..930
                     /locus_tag="ACG8MY_002385"
     CDS             <1..930
                     /locus_tag="ACG8MY_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFQ0431544.1"
                     /translation="GALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYAL
                     VALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPS
                     LKEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNA
                     IQVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHAL
                     EVPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTF
                     KIISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            984..2411
                     /locus_tag="ACG8MY_002386"
     CDS             984..2411
                     /locus_tag="ACG8MY_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0431545.1"
                     /translation="MTKKAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2424..3296
                     /locus_tag="ACG8MY_002387"
     CDS             2424..3296
                     /locus_tag="ACG8MY_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0431546.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3391..>4383
                     /locus_tag="ACG8MY_002388"
     CDS             3391..>4383
                     /locus_tag="ACG8MY_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0431547.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
BASE COUNT         1513 a          612 c          878 g         1380 t
ORIGIN      
        1 ggagcgctat ttttgtcagc tggatattta gcttctgtag gagtttttga agctgtgtca
       61 gaaaggttta gtcgtttatt tggctttgaa tatggacaag caagggcgtg gggatcattt
      121 ggttatgcct tggtagcgct tttggcagga tttttatttg taaaaaatcc tcatttaaac
      181 ttttgggcgg gatctttctt tggttcttta ctattgttaa atttattatt ttggaaccct
      241 aaagttgaac gggaagcaaa tcaaaatttt aatcaagaac aagctgaatc aaatagtatt
      301 ccttctttaa aagaaatgtt tgatctaatg aaactgcctc aattatggac gataatcatc
      361 tttattgttt ttacatggac attttatacg gtattcgatc aacaaatgtt tccgggattt
      421 tatactggtt tgttttcaac atcagctaat ggtgaaaaaa tatatgggac attgaatgct
      481 attcaagtat tttgtgaagc gttaatgatg ggaattgttc caatcattat gagaaaatta
      541 ggggttcgaa atactttgtt attaggtgta accattatgt gtgtacgaat tggattgtgc
      601 gggtttgcct cgacaccatt atctgtttca tgcataaaaa tgttgcatgc tttagaagta
      661 ccattattta cattaccaat gtttcgctat tttacacttc attttgatac aaagctatca
      721 gcaaccctct atatgatagg atttcagata gctgctcaaa ttgggcaagt gattttatca
      781 acaccattgg gaatattaag agacaacgtt ggctatcaac caacatttaa aattatttct
      841 cttattgtat tactagcagg catatatgca ttctttattc ttaaacaaga tgatagagat
      901 gttcaagggg atccatttat tcgaggataa tggaaaccaa cagaataaaa aacaatttta
      961 tctagaaagt gagcgaatat attatgacaa aaaaagctat agaattaaca aatacacgtt
     1021 atcgtttggg atatcatgtg tctgcaccat caggatggat taatgatcca aatggatttt
     1081 gctattttga tgggtattat catgtgtttt atcaacatca tccatatagt gctgaatggg
     1141 gaccaatgca ctgggcacat gctagaagta aggatttagt tcattgggaa tctttaccac
     1201 ttgctttgac tcctggagat caagaagatg aaggtggttg tttttcagga agcgctattg
     1261 aaaaaaatgg tgtgttgtat ctgttctata caggacatca ctattatgga gacaatgatc
     1321 cagatcattt ttggcaaaat caaaacatgg cgtatagtac ggatggcatt cactttacta
     1381 aatatgaaaa aaatcctgta atagcaaaag caccagaaga taacacacat catttcagag
     1441 atccaaaagt atggaaacac gatgaaaaat attacatggt tttgggtagt caagaaaaag
     1501 atggattagg tcgtgctatt gtttatcagt cgttagattt attaaattgg gattacattg
     1561 gaccaatttc taaagcgaag gaagtactta cagaaggatt tatgtgggaa tgcccagact
     1621 tttttgaatt aaatggaaaa tctattcttt tattatcccc tcagggaatt gaagcaaatg
     1681 gaaaagatta tttaaatcta tatgaaacag gctactttat cggtgattat gattatgaga
     1741 cagcaaaatt cattagagga aatttttatg aattagataa gggacacgat ttctatgcaa
     1801 cacaaacaac tttatctcca gatggtagac gtattgttat ggcctggatg gatatgtggg
     1861 aaagccctat gcctgaaaaa gtagacggat gggcaggagc attaacgctt cctcgagaat
     1921 tagagttaat tggagatcgt ttgtatatga aacctgtgag agaattagaa caacttcgag
     1981 ttggcagcgg cgttgaaacg acagttaatt tggcaggaga aataataatc gctcaagatg
     2041 cttcattcaa agaagctcta attgatgtac tattgactaa atcaaaaaat caagaaatca
     2101 aattttcatt aaaaacaaaa gatgaagagt taatcaatat tttttattct aaggataaaa
     2161 atgaatgtac cttaactcgt aacggcaaaa atgatccaag gtatggaacc attcagatgt
     2221 gtgatagatt atcacttcga gtgtttatcg ataaaagttc ggcagagatt tttattaatg
     2281 atggcgagct tgtttttaca gagcgttatt atacagaaaa tcaaccaagt atgcatttga
     2341 gtgttagtga agaagctttt gtctcgagta caatttatca tctaaaaaaa gatgcaattt
     2401 cttacgagta aggaggtttt atgatgttaa ttggtagcat agaagcaggt gggactaaat
     2461 ttgtatgtgc cgtgggagac gaggattacc gagtacaaga ccaaataatt tgtccaacga
     2521 ccacccccag ggaaacattg aaaaaaactg ttgactattt taaacagttt gatataaatg
     2581 cattaggtat tgcatcattt ggtccaatag aaattcgaaa aaattcacca aaatcaggat
     2641 atattacgtc tacaccaaag gtgggatggc gaaatgttaa tgtgttaggt tatttacaag
     2701 atgagctgaa tgttccaatg tcttggacaa cggatgtgaa cggatcagct tatggagagt
     2761 acatcatgtc cactttatcg aatgaaaaaa taaattcatt agtttattac accgttggta
     2821 ctggcgttgg tggtggagct gttgttgacg gaaaactatt aggatcgttt ggtcattcag
     2881 aaatgggcca tacgtttgta aaacgccatc ctgatgatat tgattttaaa ggaatttgtc
     2941 cttatcatgg tgattgttta gaaggcttag ttgcgggacc aacgtttgaa gcacgttttg
     3001 gaaaaaaggg tgaagacgtt tctttaacgg atcctatttg ggatgtaatg gcctattatc
     3061 tagcacaagc tgtgattcaa acaacattga ttatacgacc ggataaaata gtatttggtg
     3121 gaagcgtagt aaatgaaaaa ttgttagaaa aaattcgtgt taatgttaat aaattattgg
     3181 atgattacgt ggaaatacca ccgttaagta aatatattac gcgtccttta attaaaaata
     3241 atggatcggc aacactagga aactttgctt tgggcttgaa tcaattacaa gaataaaaca
     3301 tagaaaaaat atcgattatc aggtatagta gttctataga gttctttttt tcgatagtta
     3361 attattgcat tatttaggag agacaagaga atgcgcccaa aattagaaga tgttgccaaa
     3421 agagcaaatg tatcaaaaac aactgtttca cgtgtattaa acaatcgtgg ctatttaagt
     3481 caaaaaacga ttgataatgt ttataaggct atagaagaac tcaattatca accgaacgtt
     3541 gtagcgcgtc aattatttca aaagaaaact aacattgtcg ggttactatt tcctacagta
     3601 gccaatcctt tttttagtga actcgttgag gcattggaaa aaaagttata tgaaattggt
     3661 tataaggtat taataggcaa ttcgatgaat aataaagaaa aggaaacaaa ttatttaaat
     3721 cagttattgt ctgatcaagt agatggttta attgttggta cacataacca aggaattcag
     3781 gaatataaat atcagaattt accgattgtt gcaattgatc gagtgatgaa tgaagatatt
     3841 ccagttgttg aatccgataa ctacaatggt ggaaaacttg caaccaagtt attaattgca
     3901 caaggtgcaa aaaatattat tcatacaaac ggtccaattg acttacagac tccggctaat
     3961 cgtcggagac tagcctatga agatactatg aaagcatatc agttaattcc acgaactgtg
     4021 acattagatt tcaatatttc ttatgtaaag aaaaaacaga tttttttcca aatgtttgag
     4081 gattatccta aaatagatgg tatttttgct tcaaatgaca tcgatgcggc cctaatatta
     4141 caagtagcaa aagaaaaggg gttaaatgta ccagctgatt tattagtagt cggatatgat
     4201 ggtactttaa tgactcggag tatattacca gatctgacaa cagtaataca accaattaat
     4261 gatatagcag acacagctgt tgctattttg atgaaacgaa taaataaaga agaaacaaaa
     4321 aaggaatata ttttacctgt cacattatgg atggggaaga catcagtaaa gaaaaagaac
     4381 aat
//
LOCUS       DAZKNO010000177         4281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000177 DAZKNO010000000
VERSION     DAZKNO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.182"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(343..1008)
                     /locus_tag="ACG8MY_002389"
     CDS             complement(343..1008)
                     /locus_tag="ACG8MY_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431548.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1134..3170
                     /locus_tag="ACG8MY_002390"
     CDS             1134..3170
                     /locus_tag="ACG8MY_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFQ0431549.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3174..3812
                     /locus_tag="ACG8MY_002391"
     CDS             3174..3812
                     /locus_tag="ACG8MY_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431550.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            3840..4268
                     /locus_tag="ACG8MY_002392"
     CDS             3840..4268
                     /locus_tag="ACG8MY_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431551.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
BASE COUNT         1394 a          665 c          797 g         1425 t
ORIGIN      
        1 taaatgagca gatcatagaa atataaaaaa gaactaaaat tgatctaaat tatgtgcagc
       61 ttgactacgt accaatctat attacttact agattggtcg ttaatctatt cggatcgaat
      121 gagcaaaata tttttgtgaa tgtccatcct tgattatgat tccaaaaaga atcattggga
      181 ggctgtggcg taaatattat actataagcc agaaatacct atcattctga acttttttat
      241 ttaggaactt tcatctcaag gctttttcaa ttaatttgta cccctgtttt ttggataaga
      301 tcatgtgcat tgattttttc tatctctacg ttcatctcct acttaatgcc tatctggctc
      361 ctgagggctt ctttttaagg cgtcgtcata gtcttggctg aggatttctt ggtaatctga
      421 gggaatggct tcccaagtga gcaggcgaac gttcatgacg tgttttcctt tgtgtttgat
      481 tacagcatgg tttttctctt tttctacctc tccagcgtga aaagacagcc atgtaccatg
      541 gccaaagata tcattacata cccaacgttc gatggaattg tccacgtatc ctacagggag
      601 tgtggtatag ccgatttgtg gtttcaaaaa aggattgaat cgatcgatcg tttcattttt
      661 gtattgataa ttgataagaa aaagatagac caaacaaaag acgagcagaa agaaagatag
      721 tgtctttttg agtgagaagg gttgttttga tgaataagat ttcttgttaa caagaggttc
      781 atctggcaat gttggttcta ttgattcggt agctgattca atagaaggaa aatcaatcgg
      841 cagacctaaa gtatgatgta aaagtgacag catttcaaag tctggcattg tacgagaatt
      901 ttcccaacta gaaatcgttt tatcggaaac accaattttt tcgcctaatt ctttttgcgt
      961 aagcttacaa tttctgcggt aagttcgtat tttttctccg gtgttcatct tcttctcctt
     1021 tcgattctac agtatataga tttattctac atgacctata cacttgatag atacgttttt
     1081 ttgatgaaaa acggtatgat tttatcaaag aatcatttaa gtaagggaga gtggtgataa
     1141 tcaaaaaaaa gttcacagtt ttactcacaa tcatagtgac cggtatcatg agtggttttg
     1201 ccttggttag tataaataaa caggtactct atcggacaaa tgaatttttc catcaaacag
     1261 atacttatac tccgattcga ttgccacaac attttattgg gcaagaagaa tatgagcagc
     1321 taatcatggt atttgaaaat gtctcatcag aaactgagct ttcttatatg aaaaaaacag
     1381 taaatgaatc caatgaagta gatacatctt ggaacttcaa gatacagcca aagcttgaaa
     1441 tgacattgta ttatcaaggg gatgtcaaat cttctcacga gaaggtaccc tattctaata
     1501 cctcctttcg cgtaacgtcc attgaagaat cgatcaaaat aaaagatttt gaaggtgaat
     1561 ttttcttgat gacgagagaa aaagaagcct atgaacgggt catttcacta ttcactgatc
     1621 gattcaatca agcatttcac actgattatg ccactaatat ttttgaagac tttgaaaata
     1681 gctattcgta ttcggttatt tcaaatacag acgtatataa ttatcgagtg tatttaacta
     1741 ttggattgat tcttttattc cttttgttat catccttttt cttattgatg aataaagaaa
     1801 tcagtttatt gttgaataat gacttttcat tacatcaagt attgtggtgt ttattgggga
     1861 aaaagaaaat cattattaca ttcataattg caggaatcat tggtggtttt atttttatgt
     1921 attataagga gcaatttctt tatttgttcc agatgtacgt tttcttgttc agcggaatat
     1981 atggtatctc tttgatttct cttttattag ttgaaaaaat cgcttcgtta agagaagcgt
     2041 tcaactatat ccaatacatt ctttttacat ttggattgtt gttttctctt cttttgaccg
     2101 ttgccaatgt cgaattagca actatcgttt tttcttcttc acagttagct gtttcccatg
     2161 aaccaccaaa agaaatcgct ggtgaggact atcatgtatt ttatccagta accatcggga
     2221 aaaaccacgt ggattttatt tattccaatc gttttgcatc agcagcagat caggaattat
     2281 atgaaacttt gaataaaaac ggaagtatat tagtcaatgt atcagattac ttgaatgaag
     2341 aaaatgaaca gtttggcaga gggataaaaa taaatctgaa ttatcttaag aaatttcctt
     2401 tgattgatgt ctctggaagg ttgatccaaa taaccgaaaa agaaacgcac cctgttatat
     2461 tgatccctga acgatataga catacagatt taaaaaatga cctggcgcaa ataacagaat
     2521 attatcaaga aatcagtgaa aaagaaccga aatttctatt tataaaaaac aatcaaccca
     2581 tttatacatt tattccttct atcccctgga tcgaacatta tccggttgtt gaaatactga
     2641 cattgaaaaa cagtatgtat ttggaaagaa atattctcag cggagaaatc tatccaccgt
     2701 taaagatcaa gataggctct ttgtcgaaag aaaggttatt tgagctgatt gaagaaagtc
     2761 agttacgtga taacttgcag ttatcatttc cgtatactca gactgaagag atagctgtca
     2821 aagtgttaag cagatcattt cattacttga tacagatatt tttactcact ttcttttctt
     2881 tcttcttgtt gagctatgtc atgttatgca tatattttgt ctacaattgt cggaaaatcg
     2941 ctatctttcg ttcaagtggt tattcgctct ttgaaacgta taaagatttc ttcatgatga
     3001 acctcatcaa atggggaacg acaagtgtaa tcttcctttt tcttatagaa agagagccta
     3061 aatatctttt taatattttt ttctttagct tcattgattt tattctttca gttgttttta
     3121 ttttgtctac agaaaagaaa agtcagttat tgctgatgaa tggaggttaa caattggagc
     3181 cgttgatagt gataaaaggt gcgaaaaaaa tcattcgaaa caaaacgatt cttcatttgg
     3241 atcgtttcac tgcggatgct ggggatcttg tgactgttgt tggagaaagt ggagcaggta
     3301 aaagtacgtt attgaatata ttaggattga atgatcagtt cagttcagga caattctatt
     3361 tgttcgatca agagacgaaa caattaactg ggagagagaa gcgcttgata aaaagaaata
     3421 ccatatcttt tctttttcaa gattttgggt tgattgaata tgaaacaatc gattttaatt
     3481 tagatattgg attgaagttt tcgaatttaa atgtttcaga taagcgaaca gcaaagtatg
     3541 aggcattgaa aaaagtaaat ataaacaaga agctaagtac gcctatctac acattatctg
     3601 gtggtgagaa gcaaagaatc gcctgtgcga gagtcttatt gaagaatagc aaaatcattt
     3661 tagccgatga accgactggg tcattggata aaagaaataa ggataaagta gttgaaatct
     3721 tatcgcaaca agcttcccaa ggtacgttag ttatcgtagt cacgcatgat ccatatctga
     3781 ccaaaatagg aaacaagaag atcatgcttt aaaaatcaaa aaggaaagga tgtcgtttta
     3841 tgaaaaaagc agttattatt tgtgcactag ggataggttt tgttggagga aacgctatgt
     3901 catctgaagt ggaagcaaat tcagttcacg gtgaaatcta ttacaaatct ttaaagccag
     3961 gagagattca ttttgatgct aaaggtaata ttattaagtc aaatttacca gtagaccggg
     4021 aaatttctag aacagcaatt aatcatagct ctggaagatg ggtttattat agtgatacaa
     4081 cttgtacgag aaaaacaggt cattctaacc actatagtcg tgtctataat cgtcacggtg
     4141 caaaagcgaa agttggaaac tctattcgat cagcaaatgc aacaaaagga gtatgggcct
     4201 atgcaagtgc gacaggaaat agaactgaca cattctcatg ttggtacaat ccaaccggtt
     4261 ggtattaagt atccaaaagt t
//
LOCUS       DAZKNO010000178         4277 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000178 DAZKNO010000000
VERSION     DAZKNO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.183"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..889
                     /locus_tag="ACG8MY_002393"
     CDS             110..889
                     /locus_tag="ACG8MY_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431552.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            930..1874
                     /locus_tag="ACG8MY_002394"
     CDS             930..1874
                     /locus_tag="ACG8MY_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0431553.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1890..2669
                     /locus_tag="ACG8MY_002395"
     CDS             1890..2669
                     /locus_tag="ACG8MY_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0431554.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            2682..3380
                     /locus_tag="ACG8MY_002396"
     CDS             2682..3380
                     /locus_tag="ACG8MY_002396"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0431555.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3408..4172
                     /locus_tag="ACG8MY_002397"
     CDS             3408..4172
                     /locus_tag="ACG8MY_002397"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0431556.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
BASE COUNT         1505 a          817 c          908 g         1047 t
ORIGIN      
        1 tttctatgaa tatattagtt taattatgct tttaaaataa aaagcgctca ttctgatggg
       61 aatgaatcac atctttattt atctatcatc aatatttagg agtgaatcta tggaaaagcc
      121 gttcgtcaag ttattggcta ctgtggcggt aggcacaggc gctattgtga tttgcttgtt
      181 tggataccat ttcaataatc agcggcaaca tcatcaacgg atcaattacg cagaatctgc
      241 catcaccaat caaaaagaca ccgtgacctc tttatcaaaa gaagtagata agctgtacag
      301 tacaaaagaa aaaattttcc taaatcctga aataacagaa gaaacagttt caaatttgtc
      361 acataaacta tcttctatca aactttcggc agatgattat gacatcaagg aatcagaatt
      421 acctaaagaa gctgctgcga tccaagaaga aaagaaagca gtcttaacgc agttagagga
      481 tgcagaatca aaactgaaga tccagacagc tgtcaacgat ttatttacta aaaatgtatc
      541 aaactggcag caagctgtag acgatgtcgt catcaaagaa aaggtgacaa gtgcagatgt
      601 cgctcgtgta cgtgaaaata tgagcttttt taaagatagt ccttggaaaa cggttgttat
      661 gcaatatcta ggattcgctg atactcagat cgcgcaagtc acgcaactag atcagctatt
      721 cgatacgatg ctgaaagacg gtcaagtaac agctactgct acttacgatc aatatttgac
      781 cgcactcagt ctcatcgaac agatccggaa tgaaaaaata agagccgcat acgcgtcaaa
      841 agcagaaaca gtcgctcagc aaatgggcta tagcaatacg agctattaat aagcgagaat
      901 tcaagcaagg ctagcaaacg gaggaaacta tggcaaaacg aagaagaaga tcaagaggga
      961 aaaagatatt tctaattatt ttagcaattc ttgtcgtatt agtattagca atcgtaggag
     1021 taggagcaaa attatatcta gatctctcac attctattga aaaaacttac gaatcagtgg
     1081 aacgaaccca aggagaacga acaaaaccag ttgatctgga caaacaagaa tctttctctg
     1141 ttctattgat ggggattgat acgggagatt taggtcgggt cgatcaagga agatcagata
     1201 cgatgatggt tgccacagtc agtcctgaag acaaacagac aacgattgtc agcattcctc
     1261 gtgatacgta tgtcgacatc gttggacatg acacaaaaga caagatcaac cacgcttacg
     1321 cattcggcgg agctgccatg gcgatggata ccgtacaaaa atatgtagac atcccaatcg
     1381 atcactatgt ttcaatcaat atgaaaggtc taaaagaact cgttgatgca gttggtggta
     1441 tcgaagtcaa taatgacctc accttctcac aagatggcta tgattttacg atcggtaaaa
     1501 tcacattgaa cggggatcag gcattgtctt attctcgtat gcgttatgaa gatccaaatg
     1561 gtgactacgg cagacaagaa cgtcaaagaa agatcgtaga aggtgtagcg aaaaaagtgt
     1621 tgagtctgaa cggtgtaagc aactatcaat ccgttttgaa tgctctagag tcgaatatga
     1681 aaacagacat gagctttgat gaaatgaaaa agattgcttt agattatcgg gatgcatttg
     1741 gtacgatcaa gcaagatcag ctgcaaggag aaggcttcat gcaagacaat atctcatacc
     1801 aaaaagtcag cgatcaagaa cttgatcgtg tgaaaaaaga attaaaagat caaatgaatc
     1861 tagagaataa ataggaaagt ggagaaaaaa tggaagagac aatcagcttg aaagaaattt
     1921 ttgaaacatt acgcaaacac gtgataacaa tcctcatctc aatgtttgct ggcttggcca
     1981 tctcaggcat cgtgacgttt ttcatcttga cgccaaaata cagctcacaa gcccaattga
     2041 tcgtgacact gccgcaatca gatactgcaa atgtcaatga tgtcaatacg aacctgcaaa
     2101 tgatcaatac gtataaggat atgatcgtca gtgatctagt actaaatgaa gtcaaagatc
     2161 gtttagaaac agaagacaat gtaaaaatga ctgctggaca aatcaaagat gccatctccg
     2221 tcaatcagtc acaaaattca caaatgttct cgatccaagc aacaagcaca aatgcagtga
     2281 cggctcaaca aatcgctaat acaacgtcac aagtattcca agagaacgcc aaagacgtga
     2341 tgaatgtcga taaaatctcg atcatctcag gtgcagttgc caatacgaca cctgtttcgc
     2401 caaataataa attgaatcta gtgattggct tagtgttagg aatgatggta ggcgtaggtc
     2461 tagctttctt atttgaatta ctcgaccgga cagtaaaaga tgaaaaatat gtgacggata
     2521 cattgggctt cccgatctta gggactgtac cagaaatgtc agcgaaagaa ttgaatgctg
     2581 gtatccgtaa aaaaggacca gctccgacac aaccaaaagc tacaaacgat aaaacagcgc
     2641 catcacgcag aagccgctca agagtctagg aggaaagaaa aatggcacga acacagaaac
     2701 aaaaagcagc ccaaactcat gcagtcagtt tggtcacact cacaaacccc tcttcaccaa
     2761 tcgctgaaca atatcgtacg attcggacga atatccagtt tgcctccgca gctggtcagc
     2821 aaattaaaac gattgttgtg acttcatcag gacctggaga agggaaatcg accactgcag
     2881 caaacattgc cgttgttttt gccaaatctg gtcaacgggt gctgctagtc gatgcagact
     2941 tacgtaagcc cgtcatctat aaaacgttca agttgaacaa cgcaagcgga ttaagtaccg
     3001 ttcttagttc atcaggaagt gtagcagatg agatccaacg aacaccagtc gagaatttgt
     3061 cgattctgcc aagtggacct aaaccaccaa atccatcaga actactaagt tcgccaagaa
     3121 tggatcagat tttagcagaa gcaagacaac tatttgatgt agtgatcttc gacatgccgc
     3181 cagtagttgc cgtaacagat gcacagatca tgtcttccaa aacggatggc accttattgg
     3241 tcgtacgtga aaatatgtca cggaaagaat cattgactaa agcaaaagaa ttgctggata
     3301 tggtacaagc gcgagtcctt ggtgttgtat acaatggggc agaacacagc aaagatgctg
     3361 gctattacta cgggaattaa gaaataaaaa gagaggagac aagagaaatg atcgatctac
     3421 attgccatat cctgccagga atcgatgacg gagcagaaaa catagatgcc agtatcgcca
     3481 tggctgaaaa agcgattcaa caagggatca cacatatttt atgcacaccc catcataata
     3541 atggaaaata cagcaatgaa aaatcacaag tcatttctct agtcgcttcg ttacaagcag
     3601 aattagagaa aagacaattg cctttgacgt tattggaagg acaagaagta cgtattaccg
     3661 gaacactgat cgaagacatc gaccgagatg aaattttatt tacagattta gatgatacat
     3721 atcttctaat cgaatttccg acactggaag tgccgttata tgcagaacag ctcttttttg
     3781 agttgttaca actaggaaag acaccagtga tcgtccatcc agaacgcaac gcgcattttc
     3841 gtaaagaccc gaatcatatg ctgccattcc tagatatggg atgcttggcc caactcaccg
     3901 caccaagtta tgtcggtgta tttggaaaag acatccagaa aacagccaaa atgatggtca
     3961 aacataatct tgtgcagatg gctgcttctg atgcccatgg ggtctctaag cgtacctttt
     4021 acttaaaaga gtgctatgag cagattgcta aggactttgg taaggaaaaa gtagtacaga
     4081 tgaaacaagt cgcaaaggat ctgatcaacg gggatgaaat cagttatcca gtctatgaag
     4141 aggtaaaaaa gaagaagttt ggtttatttt agttctttat tcgtagataa aaaggaaatg
     4201 aggagagagg gaaagtatgg aaagtgaaga tttattatct cgtattagtg aagtacatcc
     4261 taatcaagtt tggttgg
//
LOCUS       DAZKNO010000179         4250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000179 DAZKNO010000000
VERSION     DAZKNO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.184"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG8MY_002398"
     CDS             complement(<1..281)
                     /locus_tag="ACG8MY_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431557.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKF"
     gene            complement(489..1415)
                     /gene="glsA"
                     /locus_tag="ACG8MY_002399"
     CDS             complement(489..1415)
                     /gene="glsA"
                     /locus_tag="ACG8MY_002399"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0431558.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1438..2865)
                     /locus_tag="ACG8MY_002400"
     CDS             complement(1438..2865)
                     /locus_tag="ACG8MY_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0431559.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2896..4110)
                     /locus_tag="ACG8MY_002401"
     CDS             complement(2896..4110)
                     /locus_tag="ACG8MY_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0431560.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1326 a          907 c          763 g         1254 t
ORIGIN      
        1 aaaaacttta aaaaataaga ttgaccgtac agaaactgtg tgagctcaga aagagcaata
       61 ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt tatacaatat
      121 tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt cacccttaga
      181 gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc acacagatac
      241 acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac acgcctccag
      301 tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc ctttttattt
      361 agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca aacaagctca
      421 caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc agcctctttc
      481 aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt caacaattgg
      541 ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc tatgccgtaa
      601 cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg tacaccaaca
      661 tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat tgatttgacg
      721 attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat tcctttattt
      781 gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga gcattgtctg
      841 aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc tttcatatag
      901 taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat ttcttcattc
      961 aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg ctcaaatttt
     1021 tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc attaacaaaa
     1081 ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt gaaggcaaaa
     1141 ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc tttgattgct
     1201 aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc ggcgtctcct
     1261 ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg tttgggatcc
     1321 acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata gtggaaattt
     1381 tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt tcatttttta
     1441 tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa taactaagac
     1501 gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag ctgcgatact
     1561 agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac ctaaacaagc
     1621 aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa gactgtcatc
     1681 tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca ggtagattac
     1741 aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc ttaatgtaaa
     1801 gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag caggaacacc
     1861 gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg ctacgtgaag
     1921 tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta aacaaacaag
     1981 aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta ataggaattc
     2041 taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg tagtagcgac
     2101 ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa acattgctaa
     2161 tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg tttctacgcc
     2221 catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg aaaaatcagg
     2281 aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga cataatggat
     2341 ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac cgatccgagc
     2401 aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc agaaaataat
     2461 caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac ccgttgcata
     2521 ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta tctcaaacca
     2581 ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga cccaagtata
     2641 gacaccgcct ttttcccatc ctttcaccgt tgccatctct gctgcgcaca atgccacagg
     2701 gataaaccat agcaaaccgc ctaataacaa gaaaaatact aagctgaagc ctgaggtcgc
     2761 gaatgtggga tattcatata atgaaacgac cattgagatg gtcatagcca aaaagccaaa
     2821 gaacgttaat ttttttgcag gaattttcgt tttgctttgt tgcatgaatc ttcctctttt
     2881 ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca atccttcttc
     2941 ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc gccgatttta aaatcactgt
     3001 tgtcagcaca taggcataca cggcagtaat gatgacacca atcacttgaa tccctaagaa
     3061 gtaccagctg cttggattcg ttagtaaatg gctgttagat gcaaagatcc ctatcagaat
     3121 cgtacctgtg aatccgccca tgccatgaac accccacaca tcaagtgcat catcccattg
     3181 tttcttttta cgaaaatcaa cagcagcatg acagacgata cccgcaatga tcccaatcag
     3241 catggcactt tttgcttcta cataaccggc acaaggcgta atggctgcca atccagcaac
     3301 ggatcctgtt agtacgtcga caaaatcaaa gccatttcct tttacttttg caatgatcag
     3361 ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat tgactgcttg
     3421 ttcatctgct cgaagtgcac caccggaatt aaaaccgaac cagccaaacc aaagcagtcc
     3481 tgtaccgata gcagctgcca taagactgct atgctgactt ctttctccta aaggaatctt
     3541 tctttgacca agtgtgagga tacatgctaa agagccgaat ccagctgtcg tgtggatgac
     3601 tgctccacca gcgaaatctc taaatcccat ctgatccaaa aatccgccac cccatatcca
     3661 gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata acaagtataa tatagccttt
     3721 taaattcaat ctccctgcaa aagctcctgt catcaaagga acggtaatca cgataaacat
     3781 cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca tgttggggaa
     3841 gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt ccaataacac cgccgatatc
     3901 ccgtccaaag gccaatccga atccaccaaa tacccacata atcgttgtta tcccaacgga
     3961 gagaaaagat tgagacatga tcgttagtat attattcttc cgtgctaatc cgccgtaaaa
     4021 aaaagctaga ccaggagtca tcaggcaaac cattgctgta cataaaatca tgaatgctgt
     4081 atctcctgta ttgataggta atgtattcat ttttttcgcc tcctttctac gattacgagt
     4141 ataaaagaag gtgtagttgt ttttttcttc tgtttttttg aattaatact tgcatttata
     4201 aaagacagac aattaataaa agcttgatgt gttgtgctct ttttcgtttt
//
LOCUS       DAZKNO010000180         4221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000180 DAZKNO010000000
VERSION     DAZKNO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.185"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..584
                     /locus_tag="ACG8MY_002402"
     CDS             <1..584
                     /locus_tag="ACG8MY_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0431561.1"
                     /translation="NDKADDMLNEEVAQNEILDFIDMKYGANQLVTQRTIQRWLEELG
                     IDCIQPRPKRNRDIRYRKSDVLKLEEIKRTNLLRKKNKELARKDIEKIATDMQKKEIE
                     YNQWLASMTDVDRRQQDIDDMYDYKVPALNKIREDKLEMCFRKLFPDVKFDIEYLEKI
                     LFTINEPSMHSDLEYGQAIVDLKEKAYIKNYKD"
     gene            998..1441
                     /locus_tag="ACG8MY_002403"
     CDS             998..1441
                     /locus_tag="ACG8MY_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003726066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFQ0431562.1"
                     /translation="METILEIVRIEQVICEAEEGVNYTIKGPKDGAEIASRFIGRDDR
                     EVFFVMCLNTKNHVVAVHRCHVGSLNASIVHPREVFKSAILNNAASIIVAHQHPSGDI
                     TPSMEDINVTKRLVEAGKLLGIEVFDHLVVNGDSKFTSLKEKGHF"
     gene            1569..2315
                     /locus_tag="ACG8MY_002404"
     CDS             1569..2315
                     /locus_tag="ACG8MY_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010721123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFQ0431563.1"
                     /translation="MTEEIIKCKTRVQKHGEVFTPKRIVNQMLDIPEIKEACENLTTT
                     FLEPSAGEGAFLVEILERKLKMVSEKYNKDLVQFENYSLLALTTLYGIELLEDNAQTC
                     VMNMFQKYYDYYKEQIQYHKGKMNQKVLDSAKRIISLNIGNGNFLTRKAVDGTPLIFS
                     EWEAINLRKNTKNIKVQRTEYTFDEIYENTQKENGKALRQPKTEPEQIDIFSFLDEPI
                     DKKEEVVKEMQYIPVKIIDVYKEEMEEANG"
     gene            2308..>4221
                     /locus_tag="ACG8MY_002405"
     CDS             2308..>4221
                     /locus_tag="ACG8MY_002405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0431564.1"
                     /translation="MDKTVIKPFQEIKYKVYGYTLPEVPNHDGYVKIGDTTRDAVTRI
                     FEQVGTAGLNPKILFEKLARKSDGKWFRDKDLHKFLILNGIEKKNFNSRADEWFYFNG
                     TLEKAEELTNKYINRDYDEIQIDDKRSDYVLRNEQQQAVEKTYEYYQSKQEPKEFLWN
                     AKPRFGKTLTTFDFIRNLNATNVLIVTNRPAIANSWFDDFHKFIAWQEPQMKFVSETD
                     ALNGKAMTRDQYLEFIYTTSHVNPTQIAFISLQDLKGAEFAGGMYKKLEWVAGLNWDL
                     LVIDEAHEGIDTRKTDIAFSQLKREFTLHLSGTPFKALAKGDFSEEQIFNWSYVDEQE
                     AKENWDPTIGSNPYESLPTLNLFTYQMSKMIEDEVSAGLTIEEEKNVDYAFDLNEFFR
                     VKENGRFEYEESVVRFLDNLSSGKFPFSENQYRNELNHTFWLLPRVNAAKALEKLLNA
                     HPTFKDYKVVLAAGDGVSLHDGAEAEALDLKENTKSFDKVRNAIEQNDKTITLSVGQL
                     TTGVTIPEWTAVFMLNNIESPSLYFQAAFRSQNPYEYEKDGKLFRKENAYIFDFSPDR
                     TLRLYDEFANNLSSGSSKTSEERKRKIKKLLNFFPVIAEDEEGTMHEIDASEVLTIPT
                     RITSREVVKRGFMS"
BASE COUNT         1600 a          563 c          858 g         1200 t
ORIGIN      
        1 taaatgataa agcagatgat atgttaaatg aagaggttgc acaaaatgaa atattagatt
       61 ttatagatat gaaatatggg gcaaaccaac tcgtgactca aaggacaata caaagatggt
      121 tagaagagtt aggcattgac tgcatacaac ctcgtccaaa gagaaatagg gatataaggt
      181 ataggaaatc tgatgtctta aagctagaag aaataaagag aactaattta ttgaggaaaa
      241 agaacaagga attagcacgt aaagatatag aaaaaatagc aaccgatatg cagaaaaaag
      301 aaatagagta taaccaatgg ttagctagca tgacagatgt tgatagaaga cagcaagata
      361 ttgatgacat gtatgattat aaagtacctg cattaaataa aattagagaa gacaaattgg
      421 aaatgtgttt tagaaagttg tttcccgatg ttaagtttga tatagagtac ttagaaaaga
      481 ttttgtttac cataaatgaa ccatccatgc attcagattt agaatacgga caagcaatag
      541 ttgacttaaa ggaaaaggct tatataaaaa attataaaga ttgaaagctc atagaagaaa
      601 gggagcaaac ccgttaatcg ttatcacaat aaagtaggat aaaatttatt tgaatttgta
      661 atagggcaag tgcagatagg gagtaggtac atccgtcaga tttttccttt tcagctcccc
      721 taattgtaat ctcctaatgc gtccgtaaag aatggacgca ttatcgctta tgtaaagggt
      781 cttcgttctt cggatataat ggcaaagcca agtggataag agtgcgtaat aaagcaatta
      841 acgctttaaa cgcactctaa tttattgggc tttactcatc atatcaacga atctcttgat
      901 agctgtcgct acttacgcca cttgacatta atcgatgcgg agattgattg aatggtgacc
      961 aaaaaggtca aaaataacac gagagaggtt gatctacatg gaaaccattt tagagattgt
     1021 acgtatcgaa caagttattt gtgaggctga ggagggtgtt aattacacga taaaaggtcc
     1081 aaaagatgga gcggaaattg catctcgttt tattggacgt gatgatcgtg aagtattctt
     1141 tgttatgtgt ttaaacacta agaatcacgt tgtcgcagtt catcgttgtc atgttggttc
     1201 attgaatgct agtatagtac atccgcgtga ggtgtttaaa tcagcgatac ttaacaatgc
     1261 agcaagtatc attgttgcgc atcagcatcc aagtggtgac ataacaccat caatggaaga
     1321 tattaatgtt acaaagagat tagttgaggc aggaaagcta ttaggcattg aagtgtttga
     1381 tcatttagtg gtaaacggtg atagtaaatt tacaagttta aaagagaaag ggcattttta
     1441 aaagggagga atctcccttt ttcttcaagt aattacggaa ctaccgaaaa gttacgttct
     1501 taagctgatt atggtaaaat ggaagcagat aatatgcgaa agcttacata taaagggggg
     1561 gggagaattt gacagaagaa attattaaat gtaaaactcg tgtacaaaaa catggggaag
     1621 tatttactcc taaaagaata gtaaatcaaa tgctagatat accagaaata aaagaagcct
     1681 gtgaaaatct tacgacaaca tttttagagc catctgctgg tgaaggtgcg tttttagttg
     1741 agatacttga gcggaaatta aaaatggtat cagagaagta taataaagat ttagttcaat
     1801 ttgaaaatta ctctttacta gccttaacta cattgtatgg aattgaattg ttagaagaca
     1861 atgcccaaac atgcgtgatg aatatgtttc aaaagtatta tgactattat aaagagcaaa
     1921 ttcaatatca taagggaaaa atgaatcaaa aagtattaga tagcgctaaa agaattatct
     1981 ccctaaatat tggcaatggt aactttttaa ctcgtaaggc agtagatggt acgccattga
     2041 tcttcagtga gtgggaagcc attaatttaa gaaaaaatac taaaaatata aaagttcaaa
     2101 gaacagaata tacatttgat gaaatatatg aaaatacaca aaaggaaaat gggaaagccc
     2161 taagacaacc aaaaactgaa ccagaacaaa ttgatatttt tagcttttta gatgaaccaa
     2221 ttgataaaaa agaagaagtt gtaaaagaaa tgcaatacat tcctgtgaaa ataatcgatg
     2281 tttataaaga ggagatggag gaagccaatg gataagacag taattaagcc ttttcaggaa
     2341 attaaatata aagtgtatgg ttatacctta cctgaggttc caaaccacga tggttatgtg
     2401 aagataggtg atacgacacg tgacgctgtt acgagaattt ttgaacaagt aggtacagct
     2461 ggtttaaatc ctaaaatatt gtttgaaaaa ctagctcgta aatctgatgg aaaatggttt
     2521 cgtgataaag atttacataa atttcttatt ctaaatggaa tagagaaaaa gaattttaat
     2581 agtcgtgctg atgagtggtt ctattttaat ggaacgctgg aaaaggctga agagttaacg
     2641 aataagtata taaaccgtga ttatgatgaa atacaaattg atgataaacg ttcagattat
     2701 gtattaagaa atgaacagca acaagcggta gaaaaaacgt atgaatatta tcaaagtaag
     2761 caggagccaa aagagttttt gtggaatgca aaaccgcgtt ttgggaaaac attaacaaca
     2821 tttgatttta ttagaaacct taatgcaacg aatgttttaa tcgtaacaaa tagacctgcg
     2881 attgctaact catggtttga tgatttccat aaatttattg catggcaaga gccacaaatg
     2941 aagttcgtat cggaaacgga tgcactaaat ggaaaagcaa tgactagaga tcagtatctt
     3001 gaatttattt atacaacttc ccatgttaat cccactcaaa ttgcatttat ttcattgcag
     3061 gacctaaagg gtgcagaatt tgcaggaggt atgtataaga aactagaatg ggtagctgga
     3121 ctaaactggg acttgctcgt aattgatgaa gctcatgaag gtatcgatac tagaaaaact
     3181 gatatagcat ttagccaatt aaaaagggaa ttcaccctgc atttatcagg tactccgttt
     3241 aaagccttag ctaaaggaga tttttctgaa gaacaaatat ttaattggtc gtatgtagat
     3301 gaacaagaag ctaaagaaaa ctgggatcca acaattggta gcaatcctta cgaaagctta
     3361 cctactttga atttatttac gtatcaaatg agtaaaatga tagaagatga agtatcagca
     3421 ggattaacaa tagaggaaga gaaaaacgtt gattatgcat tcgatttaaa tgaatttttt
     3481 agagtgaaag aaaacggaag atttgaatat gaagagagtg ttgttcgctt tttagataat
     3541 ctatcttcag ggaagtttcc attttctgaa aatcaatatc gtaatgaact aaatcataca
     3601 ttttggctgt tacctcgtgt gaatgcagca aaagcacttg aaaaattgtt aaatgctcat
     3661 cctactttta aagattataa agtcgtactt gcagcaggag atggtgttag tctgcatgat
     3721 ggagcagaag cagaagcgct tgatttaaaa gaaaatacaa aaagctttga taaagttaga
     3781 aatgcaattg agcaaaatga taagacgata acactttctg tcgggcaact gacaactggt
     3841 gtgacgattc cagaatggac agctgtattt atgctaaata atattgaatc tcctagttta
     3901 tactttcaag ctgcattcag gtcacaaaac ccttatgaat atgaaaaaga tggaaaactt
     3961 tttaggaaag aaaatgcgta tatctttgat ttttcaccag atcgaacatt aaggctttat
     4021 gatgaatttg ctaacaactt gtctagtggt agttctaaaa caagtgaaga aagaaagcgc
     4081 aaaataaaga agttattaaa tttctttcca gtaattgctg aagacgaaga aggtacaatg
     4141 cacgaaatag atgcatctga agtactaacg atacctacac gaattacttc acgagaagtt
     4201 gttaaaagag gctttatgag t
//
LOCUS       DAZKNO010000181         4189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000181 DAZKNO010000000
VERSION     DAZKNO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.186"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..601)
                     /locus_tag="ACG8MY_002406"
     CDS             complement(143..601)
                     /locus_tag="ACG8MY_002406"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0431565.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(772..1917)
                     /locus_tag="ACG8MY_002407"
     CDS             complement(772..1917)
                     /locus_tag="ACG8MY_002407"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFQ0431566.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1930..2847)
                     /locus_tag="ACG8MY_002408"
     CDS             complement(1930..2847)
                     /locus_tag="ACG8MY_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0431567.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3275..3838
                     /gene="efp"
                     /locus_tag="ACG8MY_002409"
     CDS             3275..3838
                     /gene="efp"
                     /locus_tag="ACG8MY_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0431568.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            3939..4109
                     /locus_tag="ACG8MY_002410"
     CDS             3939..4109
                     /locus_tag="ACG8MY_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431569.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1313 a          773 c          788 g         1315 t
ORIGIN      
        1 tattttatat aataaaaaga gcgctaaact gaaaactagc tatttgtttt cccgtttaga
       61 actcttttca gacaataaat atttaataaa atacgaagat aagatagcta tattgcttct
      121 catctttacg ttcttttaga aactatgctt ctacttcaga ccattcatcg cgttttgcta
      181 aaaattcacg agccaaagct tgtgcacctt ctagtgtatg gctggcagcc cagccacatt
      241 gtgtttcatt gcttgcaggt acttcagtag cttcaagaac atctttcatt gttgcttcaa
      301 gaacatctaa aatgtcttcg tagctttcat gattcaatac agtcaagtaa aagcctgttt
      361 ggcatcccat tggagaccag tctacgatgt aatctgcgtg attgcggatt agttcggcag
      421 tcaagtgctc aagtgaatgt agacttggca tgtccatgtg ttctttattt ggttgtttaa
      481 agcggacgtc gtatttaatg atgacatcgc catgcgcacc cgtttttcga tcagctacac
      541 gtacataagg tgctttgact tttgtatggt caagattaaa actttcgaca ttcattctca
      601 tttgtaagac tcctatctgt ttgttcattc gttttgatgc ttttcactaa agaattattt
      661 agtgaaattt acaaaaacgt ctttctactt gattctaaca cttatcgttt ccaaggtgta
      721 gcaatctgaa atatttttta gatttttctg ctaagtcgtt tattgggact tctagttctt
      781 gattctgtca aatgcctgag ctaaatcgtt tatgagatcc tgaccatact caattcctac
      841 agaaagacgg atcaagccgt cctttatgcc agcctcttct cgtttttctt ttggaataga
      901 cgcatgagtc atgacagaag gaatttccac taaactttct acgccaccta aactttctgc
      961 taaagtaaat agttggagtg attcaacaaa tggaatagca gattcttcat tttttaaagt
     1021 aaaagaaatc attccgctaa agccgctcat ctgctgctta gcaactccat ggagttcatg
     1081 atcgggtaat cctggatagt agaccttttc tactgctgga tgagaaaata gaaaatcagc
     1141 taccacaaaa gcattttttt gatgttcctc catacgaaca cttagtgtct tgattccgcg
     1201 ctgaagtaac cagctatctt ggggaccaag tacggctccg atagcatttt ggtaaaatcc
     1261 gatctgatcg gctagatctt tatggttaga agtcactaaa ccagctacta catcgctgtg
     1321 tccgcctaga tatttggtac cgctatggac aacgatatca gaaccgagat ctaaaggtct
     1381 ttgaaaataa ggagtagcaa atgtattgtc agcaataact atcaatccat gttgatgagc
     1441 cagagtggca cttttttcta aatcagtaat ctttagaagc ggattgctag gtgtttcaag
     1501 ataaagtgct tttgtattgg gtttgatgga ttgttctatt ttatcaagat tactcgtatc
     1561 tatgatcgtg tactcgagac catttttagt aagaactttg tcaaatagac ggaatgttcc
     1621 tccatagaca tcatctccta ataagatatg atcgccagct tgaaaaagag aaaaaacggc
     1681 atgaatccca gaaagaccag aagaaaaagc gaatcctcga acaccgcctt ccaaatcagc
     1741 aatcagttct tccaatgcaa aacgagtagg attaccagat cttgaatatt catattgttt
     1801 aggctggccg acgccatctt gccgataagt ggatgtttga taaataggta cactgacagc
     1861 tcctgttgtg ggatcttcac tgatgcctcc gtggatcaat tttgtttgaa tgtgcatgtt
     1921 tctttcctcc tataagtaaa tgttttttga taaatagcgg tctgctgcgt ctggaaaaat
     1981 cgtaacgaca cgatgaccag gtggtaaacg acgaatttct cttaatgctg ctgcaaaagc
     2041 cgcaccactc gaacttccaa ctaatagtcc ttgttctcgt gctaactgcc tagtatagtt
     2101 aaacccttct acatcagaga ttgtctcgat ctgattgatt gaaaggggag aaagaaacgg
     2161 cggaatgaat tctacaccga taccctcaat ttcgtgaggg gctggatcac caccatttaa
     2221 cacagagccc tctggttcta caccgattat tcgaatgtcg ggatattttt ctttcaaaaa
     2281 ggtagaagtt ccagcaaagg ttccaccact cccaattcct gctacaaaac tatggatatt
     2341 ttctttgatt tcatgaaaga tttctggccc taacgtatga tagtaagcag caggattatc
     2401 tttattttca aattgaagag gcagatagct gtttgatatc tcctctgcaa gttttttact
     2461 tttgctgatg gcaccgatga ttccttgctc actaggagaa tggatgatct ttgctcccaa
     2521 tgctttcatc aattgttgtt tttctaagct aaacttctct ggaacaacaa aaaccgttct
     2581 taatttgtat gtgagtgcgg ccagagcgat cccaattcct gtatttccgg cagttggttc
     2641 gatgatcgtc gttttttcat tgatttttcc ttgggcaaat ccttctttga tcaaataact
     2701 gcccaatcta tctttgatac ttcctccagg atttaaatat tctagttttg cataaatagc
     2761 agaaccttta ggcatagaat agtgctgatt gtcaaagcga taaagcggtg tggaaccaat
     2821 agcttctgta attgtcgaga taatcatgtg ggcctcctaa aaaaagataa taaaaaaagg
     2881 agctgactat taaatcagct ccttgaaaga catagagaaa aattgagtag ataaaaagat
     2941 aagttacctg gtactcattc tatgaagggc aattaataga catgacaaat agtatgctgc
     3001 ttttatgcac catacaacag caacgattca attgtttttg gttgctgact gtcatgtgta
     3061 tccctccaag taaaattttt attaacgata gcatgaaaaa aaataaaagt caaaggcttg
     3121 aaagaaaaaa atatttcgat agcatcgttt acccgtgttt atttttctga caaataggca
     3181 tgagacctta aaagatagag aatactctag ttttctggcc ggttgtctga tacaataaag
     3241 ttgattatga taaagaaaag aaggagcaaa aataatgatc tcagcaagtg atttaagagc
     3301 cggtatgact ttcgtacaag acggcaaact gattaaagtt ctggatgcaa gccaccataa
     3361 acctggtaaa ggaaatacag ttatgcgtat gaaactgaaa gatgtccgtt caggtgcaac
     3421 atacgacaca acgttcagac cagaagaaaa atttgaaaaa gcacatatcg acacaaaacc
     3481 tgtacaatat ttgtacacga tggatgatac agctttcttc atggatttgg aaacatatga
     3541 gcaatatgaa attcctgtag aaacagtaga agaagaaatg aaatatatcc tagaaaacat
     3601 ggaagtaaaa atccaatttt tcggtagcga agtcatcggc gttcaattac caacaactgt
     3661 cgtattacgt gttgtggaaa cacaaccttc catcaaaggt gcaacagtca ctggttcagg
     3721 taagccagcg acaatggaaa ctggcttagt agtcaatgtt cctgacttca tcgaaacaga
     3781 tgaattgttg gaaatcaata cagcagaagg ttcttatgta aaacgtgcgt caaaataatt
     3841 cgctaacgga ttgataagaa tttgaaaaca gagcttgacc tcttgaaagg cagatcatcg
     3901 gatctgcctt tcaagaggtt ttttgtggaa tcagcaaatt gaaaaataaa aaagtatttg
     3961 attttattta tattttgaaa gatgaatggc aaaagctaga gaaagaaaaa actgcactta
     4021 tttatgaaga aattgaaatg attcataaaa ttgaaaatat ccgaactatt ccgaactata
     4081 ttgaagcgaa aatatttttt catcactaaa tagtgttgaa tttatgtttt tatgtataaa
     4141 tagcttctaa tagttcgggt attttgtttt tataaccatt tttattctt
//
LOCUS       DAZKNO010000182         4139 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000182 DAZKNO010000000
VERSION     DAZKNO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.187"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..168
                     /locus_tag="ACG8MY_002411"
                     /pseudo
     CDS             1..168
                     /locus_tag="ACG8MY_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            406..642
                     /locus_tag="ACG8MY_002412"
     CDS             406..642
                     /locus_tag="ACG8MY_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431570.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="ACG8MY_002413"
     CDS             649..2601
                     /locus_tag="ACG8MY_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0431571.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="ACG8MY_002414"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="ACG8MY_002414"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0431572.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3445..4139
                     /locus_tag="ACG8MY_002415"
                     /pseudo
     CDS             3445..4139
                     /locus_tag="ACG8MY_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1454 a          672 c          738 g         1275 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg
     3841 gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct
     3901 acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg
     3961 ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt
     4021 ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca
     4081 gttatggaag ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacgga
//
LOCUS       DAZKNO010000183         4045 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000183 DAZKNO010000000
VERSION     DAZKNO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.188"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..891
                     /locus_tag="ACG8MY_002416"
     CDS             82..891
                     /locus_tag="ACG8MY_002416"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0431573.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            931..1428
                     /locus_tag="ACG8MY_002417"
     CDS             931..1428
                     /locus_tag="ACG8MY_002417"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0431574.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(1813..1886)
                     /locus_tag="ACG8MY_002418"
     tRNA            complement(1813..1886)
                     /locus_tag="ACG8MY_002418"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1850..1852),aa:Ile,seq:cat)
     gene            2073..2396
                     /locus_tag="ACG8MY_002419"
     CDS             2073..2396
                     /locus_tag="ACG8MY_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431575.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2560..2814
                     /locus_tag="ACG8MY_002420"
     CDS             2560..2814
                     /locus_tag="ACG8MY_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431576.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            2884..3129
                     /locus_tag="ACG8MY_002421"
     CDS             2884..3129
                     /locus_tag="ACG8MY_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0431577.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            3205..3570
                     /locus_tag="ACG8MY_002422"
     CDS             3205..3570
                     /locus_tag="ACG8MY_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0431578.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(3631..>4045)
                     /locus_tag="ACG8MY_002423"
     CDS             complement(3631..>4045)
                     /locus_tag="ACG8MY_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431579.1"
                     /translation="LSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGL
                     NRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRR
                     CSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         1260 a          711 c          719 g         1355 t
ORIGIN      
        1 gttagtttca tttttttaaa ttctcattta atcttaaggt aacaggaaaa tagatatgta
       61 aaaagaaagt aggaagataa aatgaaacta gcagcgattg atttagacgg taccttgctt
      121 gatcgaaatg gaaagatacc agaagaaaat atccaagcac ttcggaaatt tgatgaaaat
      181 ggcggaatcg taaccgtcgc taccggaagg aacagcattt ctgctaaaga tattttttcc
      241 gaattaggaa tcggtggcta tttgatttct tccaatggtt cactgattgc tgaaatgaag
      301 gatggaaaaa tggcgaaagt tctcagacgt tcaaaaatag atagcgatac actgagaaaa
      361 gccttcttct atgctcaaga agcgaaaata tccatcattg ctagccgaga aacccaagat
      421 gatcagctga cattcgatga atcaaatcta gtcaaagatg acccttatta tgaacatttc
      481 aatttacaga accattcttt tgatcaaatt ttaaatatgt tggaagatcc ttctcttagc
      541 tatcttaaat tagcattaac agataagagt gaagaaaaac tacagaagct acaaagagat
      601 ctgcaagcag atggtatcca atcagtattc tctgatccgc atttcttaga aatcacgcct
      661 acaggtatca ctaaagctca ttcacttgtt tttctggcgg attaccttgg aatcgaatcg
      721 agtgacatca tggcttttgg cgatcaagaa aacgacattg ctatgttgaa ttacagcggg
      781 ttgagtattg cgatggggaa tgcacagcga caagtaaaag aaattgcaaa ccaagtaaca
      841 ctgacgaatg aagaagctgg tgttgcttat attttaaaaa acttctatta aaaagaataa
      901 aatgacgata tgaatgtagg aggaaagaaa atgttagtta ttattgatat gcaaaatcat
      961 attcttgatc caaacagcga attttatatt ccagagtcag atcaattggc agaacgtatt
     1021 gctcaaagat tagctaaagc acgccaaaat aatgaatata ttctcttcac acgggatata
     1081 ccagttgaac tgaaggacaa agaagaagaa cgagaagatt tgcaattgat ccctcttctt
     1141 tctccacaag aaaatgaacg agtgattaaa aagtattact ttacgattcc acctgaaact
     1201 ttagctgaaa tccaggaaac tttatttgaa agcaaagaag aacaaaaaga gatcgaagta
     1261 gtcgggattg aaacaaactt gtgtgtactc tctaatctga taggcctgca gagtgctttt
     1321 cctgaagcag acttttttgt tgatccaatg ttagtctcca gtagaaaaca cggagaatcc
     1381 gctttagagc tgttgaaaga tttcaacgtc tctatcgtcg aatcataaaa atatctatct
     1441 tcatttacga tatttaacac acaataaaaa aagcacgcag aactgtttta gtcctacgtg
     1501 ctttttctat caaagtagat tggctttttt atgacacaga gtgaaacggc aggctcacga
     1561 cctctttctt aaatggtgtt caatcagctg accctcggta ttagcttaac aaacggcaaa
     1621 atatgaggaa ctatttttcc gtttgttaag ctaatactca ggtcaaaacg ctgatttcac
     1681 aacctcttcc ttaaacggta ttcaaaagct tgcatctgca gtaataagaa cagccaagta
     1741 aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg gcagcttttt
     1801 cacaacctct tatgtacctt gtaggactcg aacctacgac cggacggtta tgagccgtct
     1861 gctctaacca actgagctaa aggtacatca taaataaaat ttatttttgt tacttttcaa
     1921 ctatagcacg aatgacaaac tctcacaagt ttcaaaatat aagtaaaata atattgttat
     1981 tcttatttct tttacttgtg tcataactat tttgttactt taatttttat ttgcttattt
     2041 tagaaaaaat gttattcttt tatttattct caatgttagg agtgattatt ttgccattta
     2101 ttgcgatttt attcgattta gttgcggcag cagcttattt tctccaatta aattatcaaa
     2161 cctcaggaat tctgtttatt ggaatgattt ttcaaggtgt gattaccctt ttgcttcttg
     2221 tcatgatgct cagctataaa ggaaaaagat atgcccgtat tcaaacaaaa gtatttgtta
     2281 aatatgtttc cattcgctat gggatcatca ttctttcctt tttagtgaat gcgatcgtcc
     2341 tctttttata tgtattgaat tacttagata tcaatccatt gattttttct cgatagataa
     2401 gacacattca gcggaacagt tcctgcttat cagcagaaaa ctgttctgct ttttcatttt
     2461 tcattgatct attcttcttt tattttccta gcatttcatt tgtgatgtat gcaataacat
     2521 gttatctttt attatatgaa tcgttgagga gtccgttaca tgattacgtt ggtgagagtt
     2581 ttgttttggg tgcctgcggt cgcactggtc gcaagtattg tttatcttat gaattggaac
     2641 aaagaacggt tttatttagc tattttgact cttccggcta tctactttat gtggaaagtt
     2701 tttaattaca actactttga acccgatagt gtttttatag aagaattatc aggactggtt
     2761 ttgtctttat tgatcgtcat tctttatttg atacgtttaa ataaaaaaca ttgagtacta
     2821 ttgattgcat gtgtagcttt ctatagtcat tataactatt ctactataat gaaaggtgat
     2881 taaatgagta tcgaagaagc tattttactg gatactttac gttgtcgtct gaatttctta
     2941 agaactggcc gatctttatc tcaagtagct gtttctagcg cgttaagtat tcctttattc
     3001 aaatatcgta agtacgaaga aggagaagaa attccaagca tcattgaatt aatctatatc
     3061 gctgactttt acgatatttc cgttgattac ctaattggtc gctcagaaac agcaagcaaa
     3121 ctgaagtgat tttttccagc cttggtgctg gttttttcat ttaacgtcta aggttaacaa
     3181 tctatatata aaggagacat ttctatgcga ttatggcatg aaaaattgat ttcaaagctt
     3241 cctcgtcaac agcttttagg acaacatcgg gaatgctgtg cattaagagg aaatggctgg
     3301 ggaaagaaac atgctactgt ggattatgtt tttcactatt ctccttatag attattccaa
     3361 tatcatcagc ttgtcatgga tgaaatggaa aaacgtggtt acagcccagc accagagtgg
     3421 aaaaatccta tgtatcgtgg caaatcatgc gaagcatttt ttgatttacc tactgtagag
     3481 cgcaacgtgc cgatttatcc ggaacacgat gaagtttatc tagcagaatg tttagcaaat
     3541 ttggagcaaa aaggaattta tcttacctaa tataactaga aaagccttct atctcctctt
     3601 acttcattaa aaagctttaa ccatcttaga tcaagctaaa atgtcgtaag ttttagggtt
     3661 atcttcatcc tcacgtgcaa aactaaaagc gtactctagt tttggttcat cgccaacttc
     3721 cctagacata aatgaacatc tcctgacgtg gtttaaaagt accgcatctt cgacgtctgt
     3781 atacgatgca cagcacagat tgcttctttt gatctttctt ttgatttttc tgttgcttct
     3841 tctacacctt cttcgattac tacatcgatg cttacaattg cttctgccta atattcccat
     3901 gcatcgtcga tttaagcctc tgtagtcact gtgatcgttt ccacatttta actcctctgt
     3961 cgtggcttca gttgattttg tgatcagttc agttgctaag ccagagtcag aacaacctgc
     4021 cattgctagc gttaatattg atagt
//
LOCUS       DAZKNO010000184         3967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000184 DAZKNO010000000
VERSION     DAZKNO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.189"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..460
                     /locus_tag="ACG8MY_002424"
     CDS             2..460
                     /locus_tag="ACG8MY_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431580.1"
                     /translation="MHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVL
                     LSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLII
                     FITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(586..1263)
                     /gene="nth"
                     /locus_tag="ACG8MY_002425"
     CDS             complement(586..1263)
                     /gene="nth"
                     /locus_tag="ACG8MY_002425"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0431581.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1270..1968)
                     /locus_tag="ACG8MY_002426"
     CDS             complement(1270..1968)
                     /locus_tag="ACG8MY_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0431582.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2236..3420
                     /locus_tag="ACG8MY_002427"
     CDS             2236..3420
                     /locus_tag="ACG8MY_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0431583.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3426..3869)
                     /locus_tag="ACG8MY_002428"
     CDS             complement(3426..3869)
                     /locus_tag="ACG8MY_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0431584.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1040 a          780 c          645 g         1502 t
ORIGIN      
        1 tctgcaccat ctttttttat cgaaaacaat acctcgttat tccaagaata aaaaagctga
       61 aaaattttgt ttattattaa ataaatttac tttaattgct cctatccttg catttattat
      121 tttttctgta cttttaagca ctactcttaa aggaaaattt atggaaagaa gctctcatgc
      181 aatgattctc acttttttat ggttgttgtt caccagaatc tatatttttc tgatgagcct
      241 gaaaccgccc aaatccatta gtttatgttt agttataaat ggtatttttt tattatcact
      301 tatcattttt attacacctt tagatagata cgtcacttat ttatataatc ctttggaata
      361 ctggacttat tttataggta tattggaagg cattattttc tacatcgggt attttcccaa
      421 taaaaataac aacttttatt tttatcgcaa taaattgtaa taagttacca tcctagctac
      481 tgagtatgta catttacttt ttcatgcaaa aaagataaac gaaacagctt ttctccataa
      541 taacaagata attcatacat ttatttgcaa ttaaagaaac cacgtctaca ggcgtggttt
      601 cttttttagc gccttttctt ttagacgcat ccgatttttc ccatcttgac atattgatag
      661 caaaggacat acctcgcact tgggatttct tgccgtgcaa tgatatctac caaaaaaaat
      721 cagtgtatga tgtgtcttta cccataattc ttgtggtact ttccgcatca gtgtttcttc
      781 tacttccatg accgtagcat ctagtttgca gatcctcagg cgttttgaga cacgttccac
      841 gtgggtatct actgcgattg ctggtatccc gaatgcatcc cccaatacga catttgctgt
      901 ctttctgcct acaccaggaa gagacatcaa ttcttctctt gacgttggca cttgcccatc
      961 gaaacgttca atcagctgct gtgcacacgc tttgatgttt tttgctttat ttcgatacaa
     1021 accaatcgtt tttattttaa gaatgatttc atcgattgaa gcttctgcta acgcatcggg
     1081 tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc actgatacat ctgtcgcctg
     1141 ggcacttaag atcactgcaa tcaataactc aaatggatta ttgtgcttga gttctccatg
     1201 tgcttcggga aacattccat acatcgtttc tagagcttcc attgtttttt gtttgcttaa
     1261 catattccgc tactcctcca accaattttt cagtgagact ttcggcaatt cttcttgttg
     1321 agctgactgt tctacttctc tttgaagaag ctgctgtttt cttcgttttt ggtcttcttc
     1381 cacttgttgt tttgtacgta gattttttct ttcccacgat agcaaaatgc ggtcaacgta
     1441 attaaaacta taagcctgat ttaatacggc ttcccgcagt gctagcttca aaatttccgg
     1501 ctgataatgg tcttcttcta gccattgtcc gatccgctgg aactcgatcg aagaaagcgg
     1561 gcgtccaaat tcttgttcaa ataattgata cactgactgg atttcttttt cttgtgtttt
     1621 ttcttcgtat tttttttctt gtgtttttaa atactcacct aatagatcat agatggggta
     1681 aagattatat cgatctgctt ttttaccgtt gacaatcgtc gtttctatct tgataaatcc
     1741 atttgttacg agacgatcca gtatttgaaa aatctgatct tgtttgattc ccatatccat
     1801 agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat gcaactgtag
     1861 aacaaataaa aattcttcag attgtagacc gatcctatga taatttttaa gtaaaagatt
     1921 agatatgact gtttgtccag cgcttaagta attttctaaa ttcaacatga tttctcctcc
     1981 gctcaatcac agtatacgta aaaaaaacat gggaagcaag aacaggtttc ttttctattt
     2041 acttcactga tcggtcagat ttacaatgtc gtctacttga tttttgtttg ttcatgtttt
     2101 tgatgcgtat cttcactttt cggaataaat aaattaagta gatttcgctg ttccttagaa
     2161 tgttgacaag gaaacaaaat tactatacac tatttttgtt tccaagttaa cattattgca
     2221 taaaggagtt tttacatgta cagtttcaca aataaacatt ctaaagtttt tggatacgtc
     2281 tctgtcttat tcactggatt aggatttact atcgtcagtc ctgtacttcc gtttttaacg
     2341 ctgccttatt ctcactccat acatcagcaa gctttctata tcactttgct catgtctgtt
     2401 tatgctctag ctgccttctt atctgccccc attctcggta gcttaagtga tcattttggg
     2461 cgtcatccga tccttattat cagtttatta ggatcaagta tcggatattt gctttttggt
     2521 ttaggaaatt cgatatggat gttgttcctt ggtcgtatca ttgaaggatt gacggcagga
     2581 gaaatctcca ccttatatgc ttactttgcc gacatcacgg aacctaatga aagaaccaaa
     2641 gtttttggat ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc tattataggg
     2701 ggtctattag ctgagttggg gaattctgtt cctatattta ttggagcact ctttactttc
     2761 ttaaatgccg tttatggcta cacatttatg ctagaaagcc ttccaataaa aaaacgttca
     2821 gtagatttaa gtttctccca tgtaagacct tttcaccaat tacaacaatt attcaagatt
     2881 tcatccgtta ttcctttatt gacagcaggt tttgctgttt ggttggcagc tggctctctt
     2941 caatctatct tttctcaatt ttcgattgac gcctttcagt ggaaggctgg gttggttggt
     3001 ttaagcttct ctttgattgg aatcttggat actgtctcgc aattgttcat tatgcctcga
     3061 ttgctcaaaa aattttcaga acagcagatc acaagaatag ggatgttcag tgaaatcctc
     3121 gcctatctat tcatcacatt atctggaatt cttctactgc cgattttgtt tttatttggg
     3181 attatctgtt atggtttcgg tgattctatt tttactcctg tattcaatgg ccaactatcc
     3241 aattctgtct cagataacca gcaaggtctt gtaatgggcg gtacccaaag tatacaatct
     3301 ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt atgctgcagc accatttctt
     3361 ccgacggtat tcggattttt gcttatgatt tttgcctatt atcactacaa taaaaaatag
     3421 tagcttcatt tgctacttcg tataaattct ctgtatcgac cgataaaacg aaatactgtg
     3481 tcaaattctt cttttgacat attttcaaat acagtagcat ctcgctgtac ccatttttga
     3541 tggagttgct cgtgtctatt ccttattttt tgtccgattg gcgtcaattg aaaatagatt
     3601 tcttttttat tctcttcgct ttgataagtg tttatgatcc ctctagctat cagttttttt
     3661 gttagcttac tgatggctcc tctagtcatg taactagcat tcgctaattt tgtcacgtta
     3721 ggatcaggga ttttcccgat atgttcaatc acttctagtt catttagagt aaatcctgca
     3781 aattcttttt ccatttctgg tttattacga tgaataatct cattagctaa atccagatat
     3841 tcttctatta gcagttgtct tttatccatt tctacacctt ctttttcttt cattatattg
     3901 tttttttgtt tcctattcaa caaagataaa aatattttgt aggaaaaccc aatctgaaac
     3961 caagaaa
//
LOCUS       DAZKNO010000185         3931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000185 DAZKNO010000000
VERSION     DAZKNO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.190"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(95..685)
                     /locus_tag="ACG8MY_002429"
     CDS             complement(95..685)
                     /locus_tag="ACG8MY_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0431585.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            864..2510
                     /locus_tag="ACG8MY_002430"
     CDS             864..2510
                     /locus_tag="ACG8MY_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0431586.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2559..3278
                     /locus_tag="ACG8MY_002431"
     CDS             2559..3278
                     /locus_tag="ACG8MY_002431"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0431587.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            3757..>3931
                     /locus_tag="ACG8MY_002432"
     CDS             3757..>3931
                     /locus_tag="ACG8MY_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0431588.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLA"
BASE COUNT         1217 a          691 c          818 g         1205 t
ORIGIN      
        1 cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac tctttttaat
       61 atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt gacacagggg
      121 acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg agaacgaata
      181 gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta ttggtaagta
      241 aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc ggtatcgtta
      301 taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg aacaaaagac
      361 ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga acaatctcta
      421 aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat tttcctaata
      481 ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact gagcgttgga
      541 tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa gctatatcgt
      601 gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa aatataacgg
      661 gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca taaaaggctg
      721 tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat tgttcttata
      781 aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat agtaatgttt
      841 tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta gatcaattaa
      901 caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat atcatttcaa
      961 gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa aatgctaagg
     1021 ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta ctgatctcta
     1081 cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca ttgaatgaat
     1141 atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg ttaggcctgt
     1201 tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct ggtggcggag
     1261 aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt ccttgtatga
     1321 gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg ctgtcgcaga
     1381 ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc atcatgaaag
     1441 tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca tttatcggta
     1501 tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg tatacatcgg
     1561 cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg ttgttagata
     1621 ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc ttcaaattag
     1681 tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc aatgcacagt
     1741 tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg gtagttattg
     1801 cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct gttacgttga
     1861 aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt tcatttttta
     1921 tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca ttattttata
     1981 caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga ctcttcctag
     2041 gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag gcggcaatta
     2101 attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc attcgtttat
     2161 ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct tgtaccttga
     2221 tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg cgcaggacat
     2281 tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca aagatgattt
     2341 ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt cttgtagtag
     2401 caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg gcagaaagac
     2461 tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa gtgaatcaga
     2521 agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat aagtttttag
     2581 cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa ggaacgatcg
     2641 aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc gaagatgcgg
     2701 ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt ttgcataaac
     2761 ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc gatttattgg
     2821 aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa gacacagagg
     2881 gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca cctaaaaaac
     2941 atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa gatatccgtt
     3001 tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca aaactccaaa
     3061 ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg catgaaggaa
     3121 agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta tatttaaaaa
     3181 gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa tatcgaccca
     3241 tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag ctaaaagaat
     3301 aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact ttcggatggc
     3361 ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc gtttgttcgg
     3421 aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg actcagagtt
     3481 agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc acaacctctg
     3541 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg
     3601 aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc
     3661 ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta
     3721 taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat
     3781 cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg
     3841 ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat
     3901 tttcatattt cagcagatgg gttcttggct a
//
LOCUS       DAZKNO010000186         3900 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000186 DAZKNO010000000
VERSION     DAZKNO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.191"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..845
                     /locus_tag="ACG8MY_002433"
     CDS             <1..845
                     /locus_tag="ACG8MY_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0431589.1"
                     /translation="NKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYP
                     LWNLGADVWALNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAE
                     FGSGVSDPFISGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGF
                     VLEVPYGAKDPEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGK
                     MIYQMADENFKNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLV
                     KQNQ"
     gene            886..1797
                     /locus_tag="ACG8MY_002434"
     CDS             886..1797
                     /locus_tag="ACG8MY_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0431590.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            1794..2648
                     /locus_tag="ACG8MY_002435"
     CDS             1794..2648
                     /locus_tag="ACG8MY_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0431591.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            2645..3262
                     /locus_tag="ACG8MY_002436"
     CDS             2645..3262
                     /locus_tag="ACG8MY_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0431592.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            3264..3782
                     /locus_tag="ACG8MY_002437"
     CDS             3264..3782
                     /locus_tag="ACG8MY_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0431593.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         1139 a          667 c          791 g         1303 t
ORIGIN      
        1 ataataaaaa gttatttaaa gaagctggaa tcagtgaaca agatctaccg acaacttggg
       61 atgaattaga agaaacagca cgtaaactag ataaaaaaga aggaaaagaa tttacacgca
      121 tcggttttta tccattatgg aatttagggg ctgatgtatg ggcgctaaac gcagataacg
      181 ggactagttg gtttgacaaa gatgaaaaag tcaagattga tactaaaaat aaagtggaag
      241 ccttggagtg gatccaagac tggcaagatt attatgggaa aaatacgatc aataaattag
      301 aagccgaatt tgggtctggt gtatctgatc catttatttc cggattagta gccatgcgtg
      361 cccaaaatat caattattac tctagtttga tggaaaatgc accggatgat tttgattttg
      421 gcgtgatgca gctcccagaa aaagaagaag gttcaggcca ttggtcgtgg ggtggcggat
      481 tcgtcttgga agtaccatac ggagcaaaag atcctgaggc ctcttatgaa ttcatcaaat
      541 atctttcttc tccagaagta caagaaaaat tcggcgaaaa aagttttgat atcatggcga
      601 gcaagacagc caatgagaat ttagtgaata atgacaacct aacggataaa ggaaaaatga
      661 tctatcaaat ggcggacgaa aactttaaaa atacggtgat cacaccagtt cctttagatg
      721 ctccggatta cacgacatta gtgaatgaac agattgacca gattctttta ggaacaaaat
      781 caccaaaaga aggattggca gatgcacaaa aagcagtaga aaacttagtc aagcaaaatc
      841 aataatccat ttttgactgt ctaggtacag aggaggcaag aaaatttgaa gcgtttatct
      901 atccgaaaga aaaaagaagc aagatgggcc tatttattta tctcgccttt tattatcggg
      961 ttcttggtat ttatgctagg gcctatgctt ttttcagtta ttggtagttt caccgattat
     1021 aatttgacat ccaagatgaa tttcattggt ctagcaaatt tcaaaagaat gttcgttcac
     1081 gatgatttat tttggaaatc gttatacaac accgtttatt tcgtcatctt caatgtaccg
     1141 ctgacgacga ttttcagtgt gtttttagcc acagtactca atcaaaaaat caaaggaatc
     1201 agtgtattcc gtacagcttt ctatttaccg gcagtcttat caggagtagc agtctacatc
     1261 ttatggatgc agctgctttc tccatctgca ggattgatca acacatttct gggctggttc
     1321 catatccctg gaccagcttg gctgtttgat ccacaatgga cgaaacctgc gttgattttg
     1381 atgaaaatat ggagttctgg aggagcaatg ctactttact tagctacatt acaaaatgtt
     1441 cctagatctt tatatgaatc agcagaaatc gatggagcag gcgtttgggg aaagttcaaa
     1501 agtattactt tgccattaat cactcctatt attttttacg atgtgatcac ctctacgatc
     1561 ggttctttcc aaatttttca agaagcttat gtcatgacga aaaatggaac aggcggacct
     1621 gcaaattctt tgctttttta taatctgcat atgtggaaca aggcgttcgt agcttttgat
     1681 atgggttatg cgatggctat gtcaatgatc ctgtttctga tcgtgttggt tctgacgttt
     1741 gtcaatttga agcttgcacc taagtgggtc tattattctg gaggtgacgg caaatgatgg
     1801 attatttcaa aaagaccagt gtgaatcggc aaacaaaaaa aatactaagt tatgtcctca
     1861 tgacagtaat aggaatcatt ttgatcattc tattgctttg gatggtgttt acttcattga
     1921 aaccaatgga agaaatcgtt cgttatcctc cgaccttttt cccagaaaaa atcgtatggg
     1981 aaaactatct ggatacgatt gccgcctttc ctttttggcg ctatgcaaga aatacattat
     2041 tcatcactgt gctagtagtg atcggtaatg tcctttcaaa ttcttttatt gcttatggat
     2101 ttgcgaagct tgattttcct gggaaaaagc tgatgtttgc tttggttctt tctacgatga
     2161 tgatcccggg gtttgtaacg atgatccctc aatatgtatt attctccaag atcggctggg
     2221 taggtactta tttgcccctg atcgttccat cgttcttcgg caatgcgttc aatatatttc
     2281 tgatgcgtca attttactta tcgatcaata atgaattgat cgaagctgct gaaatcgacg
     2341 gagcgaatca tttgtatatt tggagtcatt tgatgcttcc attgacgaaa cctgccttga
     2401 ttaccattgc gatcaattcc ttcaacgcag cttggaatga tttcttaggt cctctgttat
     2461 atatccaaga tcaagaaaaa tacacgttac agattggctt gcaagttttt cagaaccaag
     2521 caacgacaca atggaattat ctgatggcag gagcaacact tgtcttgatc ccgacgattt
     2581 tgctgttctt ttttgcacaa cgttatttca ttgaagggat ggatttgaca ggaggttcaa
     2641 aaggatgatg aatcgatcga ttggtaaaaa agccaatgat tatttcttag aaatcattga
     2701 atggattcca cgtcttatcc atttacagct actatggttc ctgtgcgtct tgcctgtttt
     2761 cacattagga actgcaagtc gaacagttct gtatatgatt tatcattaca aaaaaaatga
     2821 agagaaaaag ctcggttcac tattctggaa aaagtttcgt gagaacttct ctctttatgg
     2881 aaagcaagat agtttagcaa gtgtttattt tcttcttcta ttgatcgact atcagatttt
     2941 ccgctattgg ggtgggggaa ttggctatac attgatgtat actacgttgt ttttgcttct
     3001 attaagcgcg cttctcttta gttacaaaat cttgcttcaa ctggaacaaa aagaagcttc
     3061 ttggattact gcatttttct tgttttttaa tgatttcaaa agcgcgcttt tctatttggc
     3121 aggcacgttg atccttcttg tcgcattctg gtttgcagga ccaatctatt ttgctttatt
     3181 aggtttcagt ttcttgtttt attttcaagt cctgcttttt atccatcgta cgcaagaaaa
     3241 ggttcttaaa aaggaggaat aacatgttgc gtttgattgc taaaatcgat gatagtatta
     3301 tcaacaagtt cgccaagcaa cggaaaacgg atagtcgaaa aatcaatttt ttgaaattta
     3361 tcagtaatat ctctaatggt ggttttattt ggttgctcct tgctgctgtt ttagtcttga
     3421 tacctgatac tagagaatat ggtctagcca ttcttatcgg cagtttgatc tatttttttg
     3481 ttggtttgct ttttatcaaa aaaacgacaa gacggaaaag accttatgaa aaaaatcgtc
     3541 ggcttgtcct ttacacaagt aaaccgaaga cttcttcttt tccttcagga catacagcgc
     3601 tagccttttt atcagtagct gcgttgttcg aattaagtcc agttgcagga atattagcct
     3661 ttttatttgc ttgtttgatc gcttattcga ggattttttt acttttacat tatcctactg
     3721 acattttagg aggagctatt ttaggaagtg ttattggcat cctatgcgct gccgttatct
     3781 gaattaattg tacgaataaa gcatgagtgc gtaaatgcgc atttgtgctt tatttttttg
     3841 tttcgaggaa agcgtagttg tttattcatt tgtcttaata acttaagttt tgttaaatga
//
LOCUS       DAZKNO010000187         3885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000187 DAZKNO010000000
VERSION     DAZKNO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.192"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8MY_002438"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG8MY_002438"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0431594.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACG8MY_002439"
     CDS             complement(2260..2511)
                     /locus_tag="ACG8MY_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0431595.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8MY_002440"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACG8MY_002440"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0431596.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACG8MY_002441"
     CDS             3461..3679
                     /locus_tag="ACG8MY_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431597.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1079 a          824 c          719 g         1263 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaag
//
LOCUS       DAZKNO010000188         3873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000188 DAZKNO010000000
VERSION     DAZKNO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.193"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..333
                     /locus_tag="ACG8MY_002442"
     CDS             <1..333
                     /locus_tag="ACG8MY_002442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431598.1"
                     /translation="LRLNNRKLDRLEETMNNTLNEEQTTNKNYRSKTLKDIENEIKRQ
                     RAFLAPYTQERIQTTMDSQVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLL
                     AFWLVWGE"
     gene            340..1029
                     /locus_tag="ACG8MY_002443"
     CDS             340..1029
                     /locus_tag="ACG8MY_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431599.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(1150..1236)
                     /locus_tag="ACG8MY_002444"
     CDS             complement(1150..1236)
                     /locus_tag="ACG8MY_002444"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0431600.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            1912..2862
                     /locus_tag="ACG8MY_002445"
     CDS             1912..2862
                     /locus_tag="ACG8MY_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0431601.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            2883..3416
                     /locus_tag="ACG8MY_002446"
     CDS             2883..3416
                     /locus_tag="ACG8MY_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0431602.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEQLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            3432..3602
                     /locus_tag="ACG8MY_002447"
     CDS             3432..3602
                     /locus_tag="ACG8MY_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431603.1"
                     /translation="MTRKQLLFGSILLGILGFSPMLLSVINKGNKRGELKLNPNYYNS
                     PNEELFVKETDK"
BASE COUNT         1454 a          685 c          683 g         1051 t
ORIGIN      
        1 ttacgcctga acaatcggaa attagaccga ctcgaagaaa ccatgaacaa cacgttgaac
       61 gaggaacaga ccaccaacaa gaattatcgc tctaaaacgt taaaagacat cgaaaacgaa
      121 ataaagagac aacgtgcctt tcttgcgcct tatacccaag aaaggataca aactacgatg
      181 gattcacaag tcaaaaaact ccaccaagcg ctattagacc agcgaaacca agaacaaaaa
      241 aaggctttaa ttcaacgatc gatggatatt cttgccccct tatgttgctt agtactaaca
      301 ttattagcct tttggttggt atggggagaa taaacagaca tggcattcta tttaccctat
      361 ctactcattt ctgccagtat tttaggaatt atttggctta gctataaggc ttatcaaaca
      421 agatattcat taaaaggctc taaaattcga tttaagcgat ttttattggt cggggttata
      481 tttgccttat caatcgtttt aagtggttta ttcggcgttt tagagggtaa taaacgtgtt
      541 tcaagaagca ttttattagg ggatatcact caaaagtatg aagcagcatg tgataaaaag
      601 aaaaaagagc aagcattagc acaaaaaatg gaggaattta ccgcttgtta tgaggacatg
      661 aacaatattt ttgtggatca agagaaacgt ttaacagata aaaacatgaa aaaactcact
      721 cgtttttatc aaaacttacc agaaaaacag caaaaagagg tacaagaaaa atatgagcaa
      781 gtaaaaaaag acgtgcaata ttttaaagat acgcagacag aagaagcttg ttgtaactta
      841 tttagcgata caatcccttt ttcaaccagt gaacaagaac gaaaagaacg gcaacaatcg
      901 gtcacatacg aacgttatga aacactgctt cagcaagcaa caaacataca aaatccaacg
      961 aaaaaagaaa cggcattaaa ttatctagaa agtgtcaagg aatggttgga tcagcaacaa
     1021 caaaattgaa tttgattagc aatcatgtta aactgaacct gcaaatgatc aaacattaga
     1081 tacacgaaag ccccagagag gtcggattct ctggggcttt ttttcaggtt gatgtcgcct
     1141 ttattgattt tacttcttgt ttcggttatt caaccaataa ctgaataaag agacacaaac
     1201 ttctacaaga agggggcaaa tgaaccgttc aaacattaga tacacctcct tccagtcgca
     1261 agactgtcgg ataggcgaca agttattata ccttacaaaa taaaatctgt gtggtgtttt
     1321 taagtatccc ttattttcgg gttgttaggg cttgccctga ccgcctgtaa ggcgctttaa
     1381 gaacaagata aggtttttta cccaagactg tcttaaaacg acttagacga gctatcagag
     1441 gcttccccca cctccgttac tgacttgttt ttagcaaagg agcctttagg cgactgcgca
     1501 gtacaatgtg agagcatagc gatcacactt ttttagcgac cgtagggagc aaggttttga
     1561 attggcagtt ttgaaaaatg cgcgaacctg ttggtagaaa aaagaagtgg tttttaggcg
     1621 ggaaattttg tcaccctctt ttataatctc tttttaaacc tttttttaaa cctctttacc
     1681 gtcggctgtg tccttactcc ttcaatggat acagcgtttt ataaactaca ttttgtctgc
     1741 ttttaaacta cattttgtct gcttttaaac tacattttgt ctgcttttaa actacatttt
     1801 gtctgctttt aaactctttt tttgcactca cgtatgttat aatatgtatt atcacagaca
     1861 aaacgttgtt tataacgtct gtatccattg aaggagtaag gaggaatatc tttggcaaat
     1921 gaaatcgtga aataccatca tgaattaaat accattccgt tgcggaaatt tacaccagtt
     1981 gaaatgaatc tatttttctc tattgtgtct agaatgcgcg aaaaaggcga taaaacagtc
     2041 cgtcttactt ttgaccaact aaaagattta agtaattata aagcaactgc taataatcgt
     2101 ttcattaatg atttagaaat gacttatgat aaattaatgg atttacgttt tggtaaaaga
     2161 agtgctaatg ggctcgatat agatagattc gttatgttta gtgagtttaa aatacgtgga
     2221 actgctaaag atccatatgt agacatacaa gtttacgaaa aagctttacc tttactaaac
     2281 aatttagatg agtgggttcg ctattcactc caacaattta acgaattaca aagtagctat
     2341 gcaaagacta tgtttcgatt actaaaacaa tatcgaacta aaggttttgc ttatttttct
     2401 aaagaggatt ttcatgaact attagatatt ccaaaaagtt actggaacaa ccaagcaaac
     2461 gttgataatc gtgttataaa gcctatacga caggaattaa ccgcaatctt caaaggatta
     2521 tcaatcaaaa agaaatacgg aaaagggcga ggaaaacctg tcgttggcta tcaatttacg
     2581 tttaaaccag aaatcaaaaa agcagatgat tttaataaac atgcaccaac caaagaagaa
     2641 caaaataaag cattggcttt agctgataaa caaaaagaat acgaagaacc tgctacaaca
     2701 attaacgaaa aaatcaaaca gaagaaacga gaacttgaac tcctccaaca aatcaaacaa
     2761 ctagaaaaag agaaagaagc tcttgaaaaa caaacagagt ccgcactttc gaaaaaagag
     2821 aaaaaaagtt tattatctga tttagataat ttattccagt agcaacctag gaggcaacca
     2881 gtatgcaacc acaagaaacc tttacaataa aggaattgtc agaacttttt aagatgagta
     2941 ggcaagcaat atcaaaacat atccaaaaat tagattcttc catgatagct aaaaatgagc
     3001 gtggttataa ggttgtattg cgttctggag tattgcaact tgctcgcaac ttaggcaacc
     3061 aaatgttgct agataggttg cacgagtacg aacaagacaa cgataaaaat gttaatatac
     3121 caagcaaaaa cgtggttaca aacaagttgc tagatcagtt gcgtgaagaa aatgaatttt
     3181 tacgagaaca acttgccctc agagagcaaa ataattctca tatacaaaaa ctactcgacc
     3241 agcaacaaca actcacccta caagccaaca aacaaatcga aaaactgcaa gaacaactac
     3301 agttgactta tacagaagaa gctccagaga atgactcaac gactctttct gaagcaacag
     3361 aaacaataga caaacaaatt gatactgaaa aaaaatggtg gcaactatgg aaataactgg
     3421 agggaacatt catgacaaga aaacaacttc tattcggttc gattttacta ggaattctcg
     3481 gtttcagtcc gatgttattg tcagtgatca ataaagggaa taaacgtggt gaattaaaat
     3541 tgaatccaaa ttattacaac agtcctaacg aagaattgtt tgtaaaagaa acggataaat
     3601 aataaaaaag actgctcttc cgagcagcca gttaaaaaaa taatgagagt ttattcttga
     3661 aaaaagcaac gcgcttataa agcgctacca aaaatttatc atattttatg aaaaataaca
     3721 ccccagtaaa aaatgactgg taaaaattca caaagaaacg gaaagaagca actgcagaaa
     3781 atcaagcctt aaaaagagct ttttacctag accaatccaa aatcacaaag tgagcccctg
     3841 tatcggttga tacaagggct acttggcata ctg
//
LOCUS       DAZKNO010000189         3816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000189 DAZKNO010000000
VERSION     DAZKNO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.194"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..667
                     /locus_tag="ACG8MY_002448"
     CDS             <1..667
                     /locus_tag="ACG8MY_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0431604.1"
                     /translation="LGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLELLGT
                     SFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIFAA
                     FNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQVLF
                     IGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYYPG
                     KGQ"
     gene            669..1613
                     /locus_tag="ACG8MY_002449"
     CDS             669..1613
                     /locus_tag="ACG8MY_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0431605.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1812..2456
                     /gene="pcp"
                     /locus_tag="ACG8MY_002450"
     CDS             1812..2456
                     /gene="pcp"
                     /locus_tag="ACG8MY_002450"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0431606.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2535..3812
                     /locus_tag="ACG8MY_002451"
     CDS             2535..3812
                     /locus_tag="ACG8MY_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431607.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         1215 a          670 c          850 g         1081 t
ORIGIN      
        1 tttaggcgtc ttgattgtta ttctcgggtt tgcatttaaa ttagactcga tattgatcgt
       61 aatggttgct ttagttgtta cagctttgac tggcggtctt ggcattcaag gaatgttaga
      121 acttttaggt acaagttttg taaacaatcg cgggatggcg atttttatca tcattatgtt
      181 ggcaacagga acgcttgaaa gaaatggtct gaaagaatca gcggcttcct tgatcaagcg
      241 attcaagaaa gtatctgctg gtatgattgt agatatttat gggatctttc gaatgatctt
      301 tgcggctttt aacgtgagtt ttggtggagt tgctggattt gttcgtccaa tcattttacc
      361 gatggcattg gggaccatcg aatcaaaagg gttagaagtc catcctaagc atgaagaaga
      421 actaaaaggt atggcttcag ctatggaaaa tgtctgctgg ttctttggcc aagtactatt
      481 catcggagga gcaggggcgt tgctagtcca atcaacactg aaagatctag gatatgacgt
      541 aacattggga cagcttgctt tagtggaagt tccagtggct atcacggctt tagttgtggc
      601 aagtgtctac ttctatctta aagaaaagaa attagcgaag aaatattatc caggaaaggg
      661 gcaataagat ggactttttt acaaatcctg atattttatt aggcgataaa ttactggaga
      721 tcctttatat cgtgatggga ctcgtattga tttacactgg agtaagaaat ttaacagata
      781 aaaccaatcc tcatcgttat gggtcagcct ttttctggac ggtcttaggg gtagttattg
      841 caggaggaag atggcttccg gcaatcgtga acggtggctt gatctttgcg atgaccatac
      901 cagcaattgc taagaaagtc agcaaaggag aaagccggct tccatcaaaa gaatatatgg
      961 aaaaaatgtc agacaaacta ggaatgaaaa tttttattcc agctttgagt atcggtgtct
     1021 ttgcgatctt gtttgctcta tttaccaatt taggtgcttt agtcggcgtg ggcgtcggtg
     1081 tttttgcagc catgctaatc ttgatgttct tttctcgaga taatcgacca agtacatttt
     1141 tagatgatgc agcagatatg ctgggcacag tagggccgtt aagcatgttg ccaatgcttt
     1201 tagcttctct aggtgcggta tttacaagcg ctggagttgg gacagtgatt tccaatgcag
     1261 taggtacgat cgttccagaa ggaaatgtga acatcggcat catcatttac gctcttggca
     1321 tggtgatatt tacggtaatc atggggaatg catttgcggc catcaccgtg atgacggttg
     1381 ggatcggtgc accattcgta tttagtcatg gagcgaatcc agcgttaata ggaatggttg
     1441 cattgacttg cggtttttgt gggaccctgc tgacaccgat ggctgctaac ttcaatatcg
     1501 tgcctgtagc gatgctggag atgaaagaca aatacggagt gatcaaaaac cagttgttta
     1561 ttgcgttatt tatgctagtt ttccaaattg cttatatgat tctatttaaa taatagaaga
     1621 gaggggttgt gacagaataa ttgtcgcaac ctcttctaac attgtagtgg aacactattt
     1681 ttttataaga atatattaag tttgatctgt tatgttattt aaactattgt ttcctttatt
     1741 tacgctgttc aaggaaaatg gtatgataaa agataaccat atgttaaatc aatcggacgg
     1801 aggaacaaca gatgaaagta ctagtaacag gttttgatcc atttggtggg gacaaagtga
     1861 atccagccta tgaagcagta aaaaaaatgc cagatgaaat cagcggtgct gaaatcatca
     1921 aagtggaagt accgaccgta ttcgaaaaaa gcagtcaagt agtcaaagaa gccatccaac
     1981 agcaccaacc agatgtcgtg atctgtgtcg gacaagcagg tgggcggtca gcagtctcgt
     2041 tcgaacgtgt cgcaatcaat ctagcagaag caagaatccc tgataatgaa ggaaatcagc
     2101 cattcgatac ggctttagaa gaaaacggtc cagccgctta ctttacctca ttgccaatca
     2161 aagcaatgac aaaaaatgta caagatcatg gtctaccagc gtacatctct tatacagcag
     2221 gaacatttgt ctgcaatgat atcatgtatc gtttgcttca tttgatcgaa acacaattcc
     2281 cggcaattcg aggtgggttc atccacgtgc cattctcacc cgatcaagtg atcgatcgtc
     2341 cagtgggtac gccttctatg tctttagaag acattgcttc ttctttgact tatgcagttg
     2401 aagcagcagt agaaaataaa gaagatattt ccggaaatgc cggaacaact cactaattta
     2461 gcaagaaaca ctggctggtt gctaacgtta tgttagtagc cagctttttg taattagaaa
     2521 ggaaagaaac agccgtggaa gaatggcaat ccgtctttga agaatggttt ccaaaagaaa
     2581 tcagcaaaag ctatcccatc aaaatttcta aacaatacac aagcagtcaa cgttgggaga
     2641 tctatgcaaa attaacgaaa aaacagcggg aactagtcga taagcaccgc cgatatctaa
     2701 tcagctctcg ttttatggaa gaacattatt tagctgcaac agattgggtg ttttctgact
     2761 tcaagatcaa tccgtttttt cgaacaaaac gaagccagca aaaattgtat tgtgagtgtg
     2821 gtagagaatt aaaagtgcaa tacatcgtta aatcgccaaa aaccggaaag atcctaaaac
     2881 taggcatcaa ccactttgcc gatcacctgc atgtctctcc gacagtggca gcttcgatcc
     2941 atcagggaat gacaaaagtt gatctggcct tagacgaatt attatggctt aagcagaaga
     3001 atatcgattt tcctgaagga ctatggcaaa aatactgttt tgtcttgtac caaaatcgtc
     3061 ggatgaaaca gccatattta ccggatataa aattagctca gcggctcgca gaatttcgcc
     3121 aagtagaaat gccgatttac atagcggatt atcaagcatt ggaaaatgaa atcaagaaaa
     3181 taagtgaaca catcaatggg cagtcaaaaa agcgacagat aaaaaaagaa ctatttgacg
     3241 actttgcaga agaattagta aaggatgtag aagaattctt gatcaactat cgcgcatttt
     3301 tacgtaaaga ttggcaatcc atcgtatatg aagaagtgcc agtacatcct aatgcttatt
     3361 ttgaaacatt tatttccgtg ttgagaaaga caaaacgtca acgaacacca gaagtcactg
     3421 ctcagatgga atattttgcg aaaaaccaac gattcatcca accaaaaatc tatctattta
     3481 tttggaagca atattgtcgc tatggtttta cagaagggtt cttcgacagc attcctcgga
     3541 tagtcagaaa tggtttttta aaagtattgc gtaaagaacg agaagctata caatctgctg
     3601 ataagaaaga ccgtacagtc tcaaaagaaa aatggcaact agtagtgaaa gatatccaat
     3661 ctggaaatgt gcaagagaca atcgacaaat ggaaaggaaa acattatcgt tttactgaag
     3721 ctcagaaaca agcgttggaa tattatcaga agttagaaga atcactgcgt ttcaatgatg
     3781 aggcaagaaa gtatctgaag gaattactat aaaaaa
//
LOCUS       DAZKNO010000190         3800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000190 DAZKNO010000000
VERSION     DAZKNO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.195"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..274)
                     /locus_tag="ACG8MY_002452"
     CDS             complement(<1..274)
                     /locus_tag="ACG8MY_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431608.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTML"
     gene            541..2241
                     /locus_tag="ACG8MY_002453"
     CDS             541..2241
                     /locus_tag="ACG8MY_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0431609.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            2211..2429
                     /locus_tag="ACG8MY_002454"
     CDS             2211..2429
                     /locus_tag="ACG8MY_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0431610.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2449..3339
                     /locus_tag="ACG8MY_002455"
     CDS             2449..3339
                     /locus_tag="ACG8MY_002455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0431611.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3357..3641
                     /locus_tag="ACG8MY_002456"
     CDS             3357..3641
                     /locus_tag="ACG8MY_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431612.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         1324 a          634 c          781 g         1061 t
ORIGIN      
        1 tcagcatcgt taaatcagta aagtcgatcc acttcaattc atcgaatcca cgcctgttac
       61 gagaagagga aaagaaacgc tgaatatttt ctgtaccacg gattactttc agccctgtat
      121 gggattcata ttctccagca gaagcagatg gatcaagcaa gagcaagttt tgaggaaaat
      181 gcacgatact gtttgcaaag tcagtagcag tcagcccttt tgttaaaaca gcattacttg
      241 tggtatcaat atgtacataa actgactcag tcatcatgtt cctcctctta tttcctctta
      301 atctctttta ttttacaaca tttgcattaa aaaagcttgt ttcttttatt ttaaaattcc
      361 attacaaaat cacgttttca ttgaaaacac tctcattttc aattaatttt tgtgattttt
      421 gtattttttt atttaaagga ggttttatga attgttttct tatggaatct acaaagtcga
      481 atacgagact catgaccttt ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg
      541 atggagataa aaaaagttta cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa
      601 gcaaaacaac cccttccatt ttttcaaagt catcaagaaa gtagagacgc tttttggcgt
      661 tctgcaaaaa agcaatgtgc ttttaaagca gtatcgaaaa aatataccgt ctattttact
      721 attgctaaaa gtagaggaga tgttattttt aataatctgt tgattgaagg tgaaactttt
      781 gaatggaaaa aattttttcg gtatatggaa gcttgcgctc gtttttttat taaagaaaac
      841 tgttgttttt tatttcaatc tcctctatca gaagaatggt tgcgtatttt tcataaaaac
      901 ggctatcaag gaaccacaca attaaataaa aagctggtct atcatacagc acttgtttta
      961 ggtggaggag gagcgcacgg tgcttatcag attggtgtgt ggcaggcgct gaaggaacat
     1021 gatatcaact ttgagattat cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt
     1081 ttacaaggag atatgaaaaa agctgtgaat ttatggaaaa agctgtcgac aagacaagtg
     1141 cttgcgttac cagaaatggc tgcagttgaa gaccttcgtg aacgcttcta tcgagaaaga
     1201 cgccaaatga cgaaaacagc actaattgaa ggcggggttt cttctgctcc attggaaaag
     1261 atggtcaaag acaatttgga tttgtcctta ctcaaacata atcccaaaat acgtttatat
     1321 acggtgtcta ctaaactgcc agaactagcg gaagtagtga ctgatatcca acagacaaaa
     1381 acagaagaaa taccagattg gatactcgcc tctgcttcat tttatcccgc tatggcttat
     1441 cgaaagatcg gaaaaaataa atatgcagac ggtgggtatc gcaacaaaat acctattgat
     1501 attgcgatta acaaaggagc aacagaggca tttgtcgtag atgtgcaagg gccaggccca
     1561 gcgaaaaaaa ttcgtatgcc agacattttt atccattgga aatgtcaaac attgtggaca
     1621 ctaggcagct ttctgctttt tgatagccaa aggaaccaat taaatttaca attaggctat
     1681 ctggaaatga aaaaacgatt aggctattac tatgggaact ggtatacatt tcattctgtt
     1741 aaacctgcaa gaacatactg gcgaggattt ttgtcttact taacaaaaga aattcagtta
     1801 gaattatctt ttttcaaaag cataaaattt tggcaaaaac tgcgtaattt atataagaac
     1861 agggtcgtac cagagacatg cggattggcg atgctagaac ttttagcaaa aaaacatttt
     1921 ttactgccca atgaaatcta tcaagtagac atgatgattc aaatgatctg ccaacaagat
     1981 ataaaaagta tgccggatga tttattggca caaattggtc aattaagtac tgaagaatgg
     2041 cgaaggtatc gaaaatacca gcaaaaaata caaaatgagc gcacgaaaac tgtttatttt
     2101 gattatctgc tacaaggaaa acgcaaggat cgtttgcagc atgaattgct taagcaacca
     2161 gttgacacat tactgatcct atatttatat tatctcaagg aggaacaacc atggcacaag
     2221 aattttcata tgaaatcgta gaagaaatcg ctatattgtc agaaaacaac aaggggtggc
     2281 gtaaagaact gaacctagtc agctggaatg gtcgcccgcc aaaattcgat cttcgagatt
     2341 gggcgccaga ccatgaaaaa atggggaaag gcctgacatt gacgaacgaa gagtttgaac
     2401 aactgcaaaa agcaatagaa aatatgtaat gaggaaggac cctgtcgaat gaaaagtatg
     2461 actggttttg gcaaagccat ccgcgaaaca aaagcgtatc agttagatat tgaaataaaa
     2521 agtgtcaacc agcgttttct agatgttcaa atacgaagtc ctaaaatcct gaactttgtt
     2581 gaaaacgatt ttcgtcagct tattaaacag cagttaagcc gcggtcgaat cgaagtattt
     2641 gtgaatctag cttatattgg caaaaacgaa aaacagatcg tcatcaactg ggaattgatt
     2701 gattcactta tcactcaatt gacagacgga attcaacaac gatacggtag tcaaaatcaa
     2761 gtgtcaatta gtcggatggc ggaacgcttc gcgctggacg aaaacttcgt aacggtagaa
     2821 gaaaaaccgg aagagagcat ggaagaattg gcacagttag ctctagatac attgaaaagc
     2881 gcacttgatt ctattgaggc gagtcgagaa aaagaaggtc aagctttagc tttagtgttg
     2941 aaaaagaata ctgaagaatt caaagaagta ctaaaaaaac tcgatagttt tgtagaaatc
     3001 tatgaaaagg aatttcaaat aaggtatcaa aaaaagctgg aggaatttct tggagcgaaa
     3061 gtcgatgagc aacggttgtt gacggagcta gccatcttgt tggaacgcgg agatattcac
     3121 gaggaactgg atcgtctggc gatccatgta caaaaactag atgaacttat cgaagcacaa
     3181 tgtcctgtag gtcgggaatt agatttcctg atccaagaaa tgaatcggga aatcaacacg
     3241 atcggttcca aatcaaatgc aatcgaaatt aaaaatcaag tcgtccagtc taagacgatc
     3301 ttagaaaaaa tcagagaaca agtacaaaat attgaatgac gaaagaggta gagataatga
     3361 aatattatga cgtaacgttc cacgaattaa gtggaaaaac tgtggttaaa cgggatatcc
     3421 cttctgaaaa aaacggcttt gacgtatgga aagatgcctg tgcagactat aatgagaacg
     3481 aactgtttat tttgatcaac gacggtgctt acgtgacgat gaatcggaaa tttattgttc
     3541 gaattgacac agaagaggta gaagatccga cagaaaaagc acgttctcga aaagatgaga
     3601 tcatgggtgt agtgaatact ttgtccaata tgggctttta gcaaggatgc tgaacaaaga
     3661 aatacaataa ataatcagaa ttggatttct tcgcttagct tctttgctct aggaatacat
     3721 ttaaaggttg tgacaaaatt cgtgtcacaa tcttttttta ttttgaataa gtgatgttca
     3781 aaaaccattt tctcggtatt
//
LOCUS       DAZKNO010000191         3765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000191 DAZKNO010000000
VERSION     DAZKNO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.196"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1420
                     /gene="tet(L)"
                     /locus_tag="ACG8MY_002457"
     CDS             47..1420
                     /gene="tet(L)"
                     /locus_tag="ACG8MY_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFQ0431613.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            1984..3246
                     /gene="mobV"
                     /locus_tag="ACG8MY_002458"
     CDS             1984..3246
                     /gene="mobV"
                     /locus_tag="ACG8MY_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFQ0431614.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            3491..>3765
                     /locus_tag="ACG8MY_002459"
     CDS             3491..>3765
                     /locus_tag="ACG8MY_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFQ0431615.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRA"
BASE COUNT         1279 a          518 c          784 g         1184 t
ORIGIN      
        1 attttttaat aaagatttga attaaagtgt aaaggaggaa ttaattatga agtgtaatga
       61 atgtaacagg gttaaataca accaaatttt aatttggctt tgcgttttat ctttttttag
      121 cgtattaaat gaaatggttt tgaacgtctc attacctgat attgcaaatg attttaataa
      181 accacctgcg agtacaaact gggtgaacac agcctttatg ttaacctttt ccattggaac
      241 agctgtatat ggaaagctat ctgatcaatt aggcatcaaa aggttactcc tatttggaat
      301 tataataaat tgtttcgggt cggtaattgg gtttgttggc cattctttct tttccttact
      361 tattatggct cgttttattc aaggggctgg tgcagctgca tttccagcac tcgtaatggt
      421 tgtagttgcg cgctatattc caaaggaaaa taggggtaaa gcatttggtc ttattggatc
      481 gatagtagcc atgggagaag gagtcggtcc agcgattggt ggaatgatag cccattatat
      541 tcattggtcc tatcttctac tcattcctat gataacaatt atcactgttc cgtttcttat
      601 gaaattatta aagaaagaag taaggataaa aggtcatttt gatatcaaag gaattatact
      661 aatgtctgta ggcattgtat tttttatgtt gtttacaaca tcatatagca tttcttttct
      721 tatcgttagc gtgctgtcat tcctgatatt tgtaaaacat atcaggaaag taacagatcc
      781 ttttgttgat cccggattag ggaaaaatat accttttatg attggagttc tttgtggggg
      841 aattatattt ggaacagtag cagggtttgt ctctatggtt ccttatatga tgaaagatgt
      901 tcaccagcta agtactgccg aaatcggaag tgtaattatt ttccctggaa caatgagtgt
      961 cattattttc ggctacattg gtgggatact tgttgataga agaggtcctt tatacgtgtt
     1021 aaacatcgga gttacatttc tttctgttag ctttttaact gcttcctttc ttttagaaac
     1081 aacatcatgg ttcatgacaa ttataatcgt atttgtttta ggtgggcttt cgttcaccaa
     1141 aacagttata tcaacaattg tttcaagtag cttgaaacag caggaagctg gtgctggaat
     1201 gagtttgctt aactttacca gctttttatc agagggaaca ggtattgcaa ttgtaggtgg
     1261 tttattatcc atacccttac ttgatcaaag gttgttacct atggaagttg atcagtcaac
     1321 ttatctgtat agtaatttgt tattactttt ttcaggaatc attgtcatta gttggctggt
     1381 taccttgaat gtatataaac attctcaaag ggatttctaa atcgttaagg gatcaacttt
     1441 gggagagagt tcaaaattga tccttttttt ataacaggaa ttcaaatctt tttgttccat
     1501 taaagggcgc gattgctgaa taaaagatac gagagacctc tcttgtatct tttttatttt
     1561 gagtggtttt gtccgttaca ctagaaaacc gaaagacaat aaaaatttta ttcttgctga
     1621 gtctggcttt cggtaagcta gacaaaacgg acaaaataaa aattggcaag ggtttaaagg
     1681 tggagatttt ttgagtgatc ttctcaaaaa atactacctg tcccttgctg atttttaaac
     1741 gagcacgaga gcaaaacccc cctttgctga ggtggcagag ggcaggtttt tttgtttctt
     1801 ttttctcgta aaaaaaagaa aggtcttaaa ggttttatgg ttttggtcgg cactgccgac
     1861 agcctcgcag agcacacact ttatgaatat aaagtatagt gtgttatact ttacttggaa
     1921 gtggttgccg gaaagagcga aaatgcctca catttgtgcc acctaaaaag gagcgattta
     1981 catatgagtt atgcagtttg tagaatgcaa aaagtgaaat cagctggact aaaaggcatg
     2041 caatttcata atcaaagaga gcgaaaaagt agaacgaatg atgatattga ccatgagcga
     2101 acacgtgaaa attatgattt gaaaaatgat aaaaatattg attacaacga acgtgtcaaa
     2161 gaaattattg aatcacaaaa aacaggtaca agaaaaacga ggaaagatgc tgttcttgta
     2221 aatgagttgc tagtaacatc tgaccgagat ttttttgagc aactggatcc aggagaacaa
     2281 aaacgatttt ttgaggaaag ttataaatta ttttccgaac gatatggcaa gcaaaatatt
     2341 gcttatgcaa cagttcataa tgatgagcaa acccctcaca tgcatttagg tgttgtgcct
     2401 atgcgtgatg gaaaactgca aggaaaaaat gtgtttaatc gtcaagaact gttatggcta
     2461 caagataaat tccccgagca tatgaaaaaa cagggttttg agttgaagcg tggtgaacgt
     2521 ggctctgacc gtaaacatat tgagacagct aaatttaaaa aacaaacttt ggaaaaagag
     2581 attgattttc tagaaaaaaa tttagcagtt aaaaaagatg aatggactgc ttatagcgat
     2641 aaagttaaat cagatttaga agtaccagcg aaacgacaca tgaaaagtgt tgaagtgcca
     2701 acgggtgaaa agtccatgtt tggtttggga aaagaaataa tgaaaacaga aaagaaacca
     2761 accaaaaatg ttgttatatc ggagcgtgat tataaaaact tagtgactgc tgcgagagat
     2821 aacgataggt taaaacagca tgttagaaat ctcatgagta ctgatatggc gagagaatat
     2881 aaaaaattaa gtaaagaaca tgggcaagtt aaagaaaaat atagtggtct tgtagagcga
     2941 tttaatgaaa atgtaaatga ttataatgag ttgcttgaag aaaacaagtc tttaaagtct
     3001 aaaataagcg atttaaagcg tgatgtgagt ttaatctatg aaagcactaa ggaattcctt
     3061 aaggaacgta cagacggctt aaaagccttt aaaaacgttt ttaaggggtt tgtagacaag
     3121 gtaaaggata aaacagcaca attccaagaa aaacacgatt tagaacctaa aaagaacgaa
     3181 tttgaactaa ctcataaccg agaggtaaaa aaagaacgaa gtcgagatca gggaatgagt
     3241 ttataaaata aaaaaagcac ctgaaaaggt gtcttttttt gatggttttg aacttgttct
     3301 ttcttatctt gatatatata gaaataacgt catttttatt ttagttgctg aaaggtgcgt
     3361 tgaagtgttg gtatgtatgt gttttaaagt attgaaaacc cttaaaattg gttgcacaga
     3421 aaaaccccat ctgttaaagt tataagtgac caaacaaata actaaataga tgggggtttc
     3481 ttttaatatt atgtgtccta atagtagcat ttattcagat gaaaaatcaa gggttttagt
     3541 ggacaagaca aaaagtggaa aagtgagacc atggagagaa aagaaaatcg ctaatgttga
     3601 ttactttgaa cttctgcata ttcttgaatt taaaaaggct gaaagagtaa aagattgtgc
     3661 tgaaatatta gagtataaac aaaatcgtga aacaggcgaa agaaagttgt atcgagtgtg
     3721 gttttgtaaa tccaggcttt gtccaatgtg caactggagg agagc
//
LOCUS       DAZKNO010000192         3761 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000192 DAZKNO010000000
VERSION     DAZKNO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.197"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..855
                     /locus_tag="ACG8MY_002460"
     CDS             <1..855
                     /locus_tag="ACG8MY_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0431616.1"
                     /translation="QLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFLALVFEDQ
                     SYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIPALIEEKR
                     SGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKEWMLPNEIRK
                     KNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLLGSWQRAT
                     KRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYHKVIPASW
                     SNGTVSLP"
     gene            935..1909
                     /locus_tag="ACG8MY_002461"
     CDS             935..1909
                     /locus_tag="ACG8MY_002461"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0431617.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            2089..3234
                     /locus_tag="ACG8MY_002462"
     CDS             2089..3234
                     /locus_tag="ACG8MY_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0431618.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSCQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLEQLVRAVKK"
     gene            3371..3715
                     /locus_tag="ACG8MY_002463"
     CDS             3371..3715
                     /locus_tag="ACG8MY_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0431619.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         1280 a          665 c          824 g          992 t
ORIGIN      
        1 caactgaatc aagtagtgga taaaccttta tatttcaaag tagataaaaa aattaaaaaa
       61 ttagccagca ctcagcagct gcagtcaaga aaaagtaaat tgcttttttt agctctggtt
      121 tttgaagatc aaagttatgt gattatcgat caatcaggac atccggtaga atattctcca
      181 gcaaaatata cttatcaaga gggtctttct tgtaaaaaat ggaagctgat caatgaagag
      241 ccaatcgagt tatctagatg gatcaacaga aaagaagata ttcccgcttt gatcgaagaa
      301 aaaaggtcag gaaaggaact cgcaaattgc tgggtgggat tgccagaaga acgttttttg
      361 aggtataaga aatgggctac cccctcaggc tatctttgtg gtacttacgc ggcagcagtt
      421 ctccttgcct attatcaaga ctatcgtaag gagtggatgc ttccaaatga gattcgcaag
      481 aaaaatacgg ctgattcgtt ggttttgacg aaggcattgc gtagccagat ccaaccacta
      541 ggacttccta cgattccttt tcaggttagt acagggattt ctagtttttt aaagaagaat
      601 ggcaatcatg agcgtgcacg agcaactctt ctaggttcat ggcagcgagc tacaaaacgt
      661 atacgtgaag gcaaacctgt gatgattggt attttgaaag tacttggcag tacatatggc
      721 aatcattggg tgacagcgta cgcttatttt gaaactgaaa caggagaacg ttattacaaa
      781 gtacacgaca actggggcga ttatcataaa gtcattcctg ccagttggag taacggaaca
      841 gtttcacttc cttaagaatt tgctgaaatt aacagaaaat cttggaaaaa tatgttaaac
      901 tatcgaagaa tagagaagtt ttggggagga ctacatgagt aataagtatc aggatgacac
      961 gttgaagatt tttagtttga atggcaaccg tccattagct gaaaaaatcg cgaaagtgtt
     1021 cggaacagaa ttaggtaaaa gcgtcgtgaa gcaatttagt gatggcgaga tttccatcaa
     1081 tatcgaagaa agtattcgag gggatcatgt gtatatcgtt caatcaacga atgcacctgt
     1141 caatgactac tatatggaac tactgatcat gatcgatgca atgaagcgag caagtgcgaa
     1201 aacgatcaat gtcgtattgc cttactatgg gtatgctagg caagaccgca cggcgaaacc
     1261 tcatgaaccg atcactgcga aattgatcgc taacttgatc gaagaagcag gggcaactcg
     1321 agtactgact ttagatctgc acacggttca agtacaagga ttctttgata ttcctgtaga
     1381 taatttattt acgatgccgt tgtttgctca ttattaccgt caattaggtc tgacgggaga
     1441 tgacatcgta gttgtttcgc caaaaaacag cggtgttcag cgtgcacgta gtctttcaga
     1501 atatttgaac agcacattag ccatcgtaga tcatgcagat gatgcttcaa ctgagggtgg
     1561 ttatgtgatc ggtgatgttc aaggaaagac atgtatcatg gtagatgata tcttgaacac
     1621 aggagcaacg cttgcacgag cagcaaatgt attaaaagaa aacggcgcaa aagaagtata
     1681 tgcctgtgcg tcacatggat tattgtccga tcatgcgaaa agtattttag acaatgcacc
     1741 aatcaaagat atttgtatca ctgattctgt tttcacagaa gaaaatcgtc atccagaaaa
     1801 cttaacaatc gttacatgtt cagaattaat gggagaagcg gtcaaacgca tccatgaaaa
     1861 tacaccgatg agtccgttat tccgtctgga agaaaaagaa ttcgaataaa caaaaaaaag
     1921 agggaaagaa gttgtgacac aagtgagcag cttcaaggag ctgcgacaag acttttgtca
     1981 tagctccttt tttctatgcc catgaaacgt cctctagaca aaaatgagct cttctttggt
     2041 ttgttcgcta ttttatgcta gaatgtgcct agcaataatt tggaagggat gaatgaagtg
     2101 aaaactattt atctggatca tgcggcaact acccccatgc atccacaagt aattgaagcg
     2161 atgacggcaa tcatgcaaga cacgtttgga aatccttcaa gcatacatgg atttggtcgc
     2221 cacgcgcatg aaaaattgga aacagcaaga caaatcattg cagacagtct gcaagcaaaa
     2281 cctcacgaga tcatctttaa cagtggggga acagaagggg ataatacagc tatcttagaa
     2341 acagcgttct cgtgtcaaaa agaaggcaga catctaatca ctacagctat cgaacatcct
     2401 gctgttctta ataccatgaa atatttagag acaaaggggt ttgacgttac atatctgcct
     2461 gtagatgaaa aagggaatct gtcgatcgaa gaggtcaaaa aagcgttacg agaagatacg
     2521 atcttggtct ctgtgatgta tgggaacaat gagatcggaa atttgatgcc aatcaaagaa
     2581 atcggtgaac ttttagcgga tcatccagct tatttccata cagatgcggt acaagcgtac
     2641 ggaaaccaaa cgatccatcc agaagaatta ggaatcgatt tattaagtat ctctgctcat
     2701 aaaatcaatg ggccaaaagg cgtcggtttc ttatataaac gagacggagt aaatatccct
     2761 gcattgcttt taggcgggga gcaggaagaa aagcggcgtg caggtacaga aaatttagca
     2821 gggatctggg gtatggcaaa agcagttgaa ctgctgtcac ctgaagaacg tgaagcacgt
     2881 aacaacaaat ataaaggatt ccagcaattg attcttgata cattagaagc aaacgaaatc
     2941 ccttatcaag taaatggtga tttaacgaat aaactccctc atgtgttgaa tcttcgctta
     3001 attggcgaag caaatgattt gctactgatg aagcttgatc ttaatggggt cgctatttct
     3061 actggttcag cttgtacagc aggtaatatc gagccttcac acgtattaga agcaatgtat
     3121 ggagaagata caccgatact aaaagaatcc atccgtatca gctttggttt gggaaatacc
     3181 gaagacgaca tccagtattt ccttgaacaa ttagttcgtg ctgtcaaaaa ataactagtg
     3241 aagtcattct tatatgaaca gatgtctgag agaacgcctt cattttttag ggaacttaac
     3301 tgaaaaacct agtaaaaatc aggataaact tttataataa agagaaacta attttatcga
     3361 ggtgaaaatc atggcatttg aaacaacagc aacggtactg ggcgcaccaa ttagctatcg
     3421 tttgcatcct gatgcaaaaa gatatacatt gcgcgataat ggatttacag aaacaaacgg
     3481 aggcaattat caattgatcc gaccattaga tgccacgccg caaagcaaag aaggattcaa
     3541 attaaaaatc actgtatcaa aagatattca aacattgaaa atgtcgatca ctacagcaaa
     3601 tggattgaaa gcagtcaata ttttcaaaga tccaaaacag aaaatgagcc aagataaatt
     3661 ttattttctg atggatggga tgattagccg tgggttattc gaaaaagtcg aatgatctgt
     3721 ggatataaaa agacaactag ttttgaaacg gcctattgtt a
//
LOCUS       DAZKNO010000193         3732 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000193 DAZKNO010000000
VERSION     DAZKNO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.198"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..2557
                     /locus_tag="ACG8MY_002464"
     CDS             116..2557
                     /locus_tag="ACG8MY_002464"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0431620.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2887..3591)
                     /locus_tag="ACG8MY_002465"
     CDS             complement(2887..3591)
                     /locus_tag="ACG8MY_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431621.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1203 a          654 c          815 g         1060 t
ORIGIN      
        1 taaaaaagag aatatttaag gattgttaaa atttaatctt agaaaaaaat aactggaaca
       61 agagctattt gtttttacag caaagaatgg tatatttttg taggaggtgg cttacttgaa
      121 agaagtcgtt atgatagatg ctgcaagaac gccgatagga aagtatcgcg gaagtttaag
      181 tccatttact gcagttgaat taggcacact tgtgactaaa ggattattag ataaaacaaa
      241 actgaaaaaa gataaaatcg atcaagtaat atttggaaat gtcctgcaag ctggtaatgg
      301 tcaaaatgtt gccagacaga ttgctttgaa cagcggtctc cctgtagatg tcccagcaat
      361 gacgatcaat gaagtttgcg gatctggtat gaaagcagtg atcttagcac gccagctgat
      421 ccaattaggt gaagcagaac ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc
      481 tatgctgaaa cagtatcagt cagaaacaaa tgaatatggt gaaccaattt ccagtatggt
      541 caacgacgga ttgactgacg cattttcaaa tgcacatatg ggattaaccg cagagaatgt
      601 tgcaacacaa ttttctgtga gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt
      661 gaaagcagca catgctgtcg aagccggtgt attttctgag gagatcatcc cagtcaagat
      721 ttctgatgaa gacgtgttat ctgaggatga agcagttcgt ggaaatagta cattggaaaa
      781 actgggcacg ttacgtacag tattctcaga agaaggaact gtaacagcag gaaatgcttc
      841 cccgttgaat gacggtgcct ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa
      901 taatctgcct tatttagcaa ccatcaaaga agtagcggag gtcggtattg acccaagtat
      961 catgggaatc gctccaatca aagccattca aaaattgaca gatagatcag gaatgaattt
     1021 atcaacaatc gatctttttg agatcaatga agcatttgca gcttcttcta tcgtagttag
     1081 ccaagaactg cagttagatg aagaaaaggt caatatatat ggaggagcaa tcgcattagg
     1141 ccatccaatt ggtgcaagcg gtgcacgtat cttgactaca ttagcttacg gattgcttcg
     1201 tgaacagaag agatacggta ttgcttcatt atgtattggt ggaggtttag gcttggcagt
     1261 cttgttagaa gcgaatatgg agcaaacaca taaggatgtg caaaaaaaaa agttttatca
     1321 gcttacgcca tctgaacggc gcagtcaatt aatagaaaaa aatgtattga cacaagaaac
     1381 tgcgttgatt tttcaggaac aaacactttc tgaagagctc tccgatcaca tgatcgaaaa
     1441 ccaagtgagt gaagtggaaa tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa
     1501 gaaaaaatgg attccgatgg caacagagga accatctgtc attgcagctg caagtaatgg
     1561 tgcaaaaatc tgtggaaata tctgcgcaga aacacctcag cggctgatgc gagggcaaat
     1621 cgttctatca ggaaaatcgg agtatcaagc agtgatcaat gcagttaatc atcgaaaaga
     1681 agagctgatt ctttgtgcaa atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt
     1741 tcaagatatc tctactcggg aattcatggg gagtttccat gcatacttat ctattgattt
     1801 tttagtggat gtaaaagatg caatgggcgc taacatgatc aactcgattc ttgagagtgt
     1861 ggcaaacaag ttacgggagt ggttccctga agaagagatt ctattcagta ttttaagtaa
     1921 ttttgctaca gaatcattgg cttctgcctg ttgtgaaatc ccattcgaac gcttagggag
     1981 aaacaaagaa atcggtgaac aaattgccaa aaaaatccaa caggcagggg aatatgcgaa
     2041 gcttgaccct taccgtgcag caacgcacaa caaagggatc atgaatggta tagaagctgt
     2101 agtggcagct acaggaaatg atacacgtgc ggttagcgct tcgatccacg cctacgcggc
     2161 aagaaatgga ttataccaag gattaacaga ctggcagatc aaaggggata aactagttgg
     2221 aaaactaacg gtgcctttgg ctgttgccac agtaggcgga gcttccaata tcctgccaaa
     2281 agcaaaagca tctttagcta tgttagatat tgattcagcc aaggaactag cacaagtaat
     2341 tgctgcagta gggttagctc aaaacctggc tgctttgcgt gcattagtta cagaaggaat
     2401 ccaaaaaggc cacatgggac ttcaagctcg ttcattagcg atctcaattg gggctattgg
     2461 tgaggaaatc gagcaagttg cgaaaaaatt acgtgaagca gaaaagatga accagcagac
     2521 agctatacaa atattggaaa aaatccggga aaaataaaaa agcaatatct ttagaagaat
     2581 aaagcaagat ttacagttat tttatttact gcagatcttg cttttttctc tatcagaaaa
     2641 tatgtttatt attagaattt tttatagtta aggttaggca tcttaagaaa tcaggcaact
     2701 ccaagatatc ttcaggtaga agagaagtcg cctaacattg taggatcata ggtgatttgt
     2761 tggacaggtc aagctgatat ttgagcctga tgttagaagg aatttctttt tacttgctgt
     2821 agaaattatt atatgactaa aaaggctggg attttaaatc ccagcctttt atgacaaata
     2881 gaatttttat cgatcagtaa tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt
     2941 caatttgact ttgtatttcc gttcgagtgc tttttcgata acgaactcat tttcagtagt
     3001 catagaaata gttgctaatt tacgaggttc atcagcattt ttttcattaa taactacttc
     3061 atgttcattt tgttctgtgt aaaatttagg atactcagca acggcttttg aaaaatcagc
     3121 aaaacttaaa gcggcattcg tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg
     3181 tttttccgtt ttttcttctg gcacactgac tgtttcttct gtaactggtg tcgcaacagc
     3241 ttgcgcagtt ggttcatcgt ttttcaatcg ttgatcattc cagtaagaca cgatttcaat
     3301 aagaagattg tcacggaatc gttcgaaaat cccgttttct cctgaacgaa ttctctttaa
     3361 tacagtattg aagttgtcat cgattggcac gttgatccgt gtatcttcat ttgttttccc
     3421 agctaatccg atcgttaaga tttgacggtt tttgggatca agcatgactt gtaacggcaa
     3481 accataacgt cgcaagctct cttcttctag agaacggaat aattgtcctc ttacaccatc
     3541 caatgaagga cggaattgat ttaataattt ttgtttctgt tttttcttca tatgttctct
     3601 ccttcgctca taccctttcg attataagaa aaaaacagcc aaatgaaaag gagtaatgca
     3661 aaaaaacttg cgtatacgca aattacaaca gaaaaatttg cgtattttcc cgttttttga
     3721 aagaaaaata aa
//
LOCUS       DAZKNO010000194         3435 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000194 DAZKNO010000000
VERSION     DAZKNO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.199"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..1139
                     /locus_tag="ACG8MY_002466"
     CDS             93..1139
                     /locus_tag="ACG8MY_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0431622.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            1141..3294
                     /locus_tag="ACG8MY_002467"
     CDS             1141..3294
                     /locus_tag="ACG8MY_002467"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0431623.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         1068 a          672 c          664 g         1031 t
ORIGIN      
        1 ttatgtctac gctttcaaaa aatatctttt tatttacttt ttcatccatt atggtattat
       61 ttgaatatga tatatcatta agaggtgttt atatgaatca accattgtac cagacaatat
      121 ttaatgattt gcaaacggct attttaactg gtgaattacc tgtagacagc caactaccaa
      181 ctgaaaaaga actttcacgt acctataaag taagcagaat cacttccaaa cgtgcattga
      241 ctgaattaga gcaattagga cttatttatc gcgttcgcgg gaaaggcagt ttcgttaaat
      301 ccgcttctcc ggaacagatt cttgcttctc ctacaaaaac acgacggatt ctgtttcttc
      361 ttccatatgt cgctgatttg tctgttggtg attttagcaa gggattgaac cctgtgatgc
      421 aagataaaca gattgatgtc atgatgacga cgcttgactt tctacaaaat aaaacagcaa
      481 aagagctaat caatgaattt gacggattga tttattatgc tcttgataca gaacaacatc
      541 ttgacttact ttttgagctt tcattgaaag aattccctgt cgtcatatta gataaaaaaa
      601 tctatgagtt accttttcct accgttcttt ccgataattt ccaaggcggt gcgcttgctg
      661 cctcttattt gatcgaacag ggacattcaa aaatcgctta tttgtttggt acgcagactc
      721 atccacaatc cgttcgacag cgttaccttg gttaccttga agcattggac aaagctggtt
      781 tgactttcca cacttcactg gatgacaaaa gcgcgacatt agatgaattg ctttcttata
      841 tcactaagta tgaagtaact gcttttgttt gtgaaaacga tgttgtcgct atccagacca
      901 tgaatctctt gagacagcat ggttatcata tccctgaaga tttttcaatt atcggatttg
      961 atgatatcca agcagcagct cttgttgacc cgccactgac aaccatcgca caagattttg
     1021 aacagttagg taagcttgcg ggagatgctt tggtcacttg gttagagaca aaagatcgac
     1081 catccgatag taaatttcca gtttcattca tcaaaagaca atcaacaaag gagaaataaa
     1141 atgaatatcg gtcaaataga tactagacat gcaacagcga atcagccaaa tttctctaat
     1201 gggaactgcc agccttatac tggcgttcct tttgggatga attattttgc accgcaaact
     1261 acggatcaaa acggcagctg gtggtttcat cctgatgacc gcgtttttca aggctaccgt
     1321 cttacccatc aaccaagtcc ttggatgggt gatttcagtc atatgctgtt tacgccgatc
     1381 aatggaaaac tacaggagaa cacccttttc catgctcaaa gttcttaccg cccagaagaa
     1441 agtatatttt gcccgactca tctgtcgatc tgtcaattac gttatggcat ctgttctaca
     1501 ttgataccta gcatgtatgg cggaatctta tctattgatt acagcaaaaa tgacagcggt
     1561 ttacttttat cctttccggg acgccatcag ctctttgtta tagatcctta cacagtggaa
     1621 ggaaaaatca cacattttgc tggttcagaa gatcctgatt tttcttttta ttttatattg
     1681 agatttgaat atcctttaac aattgatgag ttagctgttt ccaacaaaaa tagcgactcg
     1741 atcccgttgt attttgaaca gacaaaaaaa caaactatcc gatttgggac atcttttatc
     1801 agctcggaac aagcagctta taacctggaa ctagaacaaa accaaagcaa agagacttac
     1861 cttgctcaca gtcaagaaca atggcagtcc tattttgatc gtatacagct cacacaccat
     1921 gatcaacaac aattgcggac gttttatcat aatttatatc gggtgttttt atttccacaa
     1981 acattttatg aaattgatcc tagtgggcaa aaaatccact atgatacgac tagtcgatct
     2041 ataaaacctg gtgttctcta tacaaataat gggttttggg atacgtataa aacggtttat
     2101 cctctttatt ctttgattgc tgtagaaaaa tacgaagaaa tgctggaagg ttttttgaac
     2161 agctatagag aaagtggcta tttgccaaaa tggctgtctc ctgatgaacg tggacttatg
     2221 cctgggacat tgatcgatgc ggtcattgca gatgctgctg taaaagatat ccgacgagac
     2281 ttgatgcctg aatttctaac ggcaatggaa aaaggtgcta cggttcaaag caaacatcca
     2341 aattatggac gccaaggaac agaagattac ttgacatatg gctatgttcc taatcactac
     2401 cacgaatcag tcaatcacac attagactat gcttatagcg atttttgtat tagccaagtt
     2461 gccaaggtac tagaagaaga ggaaatgacg aagaaatatc agcagcaatc attgaactat
     2521 caaaatatct ttgactatga gtcaggattt atgcgttcaa aagataaaga tggcaagttc
     2581 agagaaccat tttcagctaa tcgttgggga caagattatg cagaaggaag tgcttggcaa
     2641 agcagctttg ctgtttatca cgatttttca ggactgatcg gaaaatatgg tggcagtgag
     2701 agatataaag aaaaactcat tgagctgtgc aatcagcccc caacgtttga cccaggagga
     2761 tatggttttg agatccacga aatgagtgag atggccgcga tagaatttgg acaattagct
     2821 atttcaaacc agccaagttt tcactatcct tacttgttca gttatatagg acaacctgaa
     2881 atggcacagc cactgcttaa acagttgatg actcagacct tcgacgattc tccaatgggt
     2941 taccctggag atgaggacaa tggaagtatg gccgcttggt atatcttcaa tagtctggga
     3001 ttttatcctg tcacgcctgg aaccggtgaa tatgttatcg gtatgccatt aattgacaag
     3061 gctgttttgc ttttgtcaaa tggaaaggaa ttgacgattg aagcaacgcc taatcggccg
     3121 cagcaacaat ttatccataa aatcaaacgg atggacaaag aatatacgaa attatatttt
     3181 acccatcaag atttgctgaa aggcggcacg atctcttacc aattagggat catccctcac
     3241 cctaagaaat atcaagcaaa agatttgcct ttttccttat caaactataa ttaacaagag
     3301 gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caagcaaaaa tagatcctca
     3361 tatttttcgt ttggttggtc ttattaccgc agatgcaagc ttttgaacac cgtttattga
     3421 gaggttgtga aatca
//
LOCUS       DAZKNO010000195         3339 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000195 DAZKNO010000000
VERSION     DAZKNO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.200"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(480..842)
                     /locus_tag="ACG8MY_002468"
     CDS             complement(480..842)
                     /locus_tag="ACG8MY_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0431624.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            1065..1817
                     /locus_tag="ACG8MY_002469"
     CDS             1065..1817
                     /locus_tag="ACG8MY_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0431625.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1957..2880)
                     /locus_tag="ACG8MY_002470"
     CDS             complement(1957..2880)
                     /locus_tag="ACG8MY_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0431626.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2895..3167)
                     /locus_tag="ACG8MY_002471"
     CDS             complement(2895..3167)
                     /locus_tag="ACG8MY_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431627.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1097 a          625 c          542 g         1075 t
ORIGIN      
        1 tggtatttaa cctaaacgag aaagaatact tattttattc aatgtatccg agacacgaag
       61 aaattagcgt tttctatttt tcttacttga taaaataagt agattttcct actacaatcg
      121 tagccgttaa gataccgatg attgaacaaa tataaccaaa tgtctctaca acataatcaa
      181 tggcaaaagt ttttgaagca ataatacgtc tttccttttt tgcttcttat ccccaaagaa
      241 acggatataa cttattatgt attgaatttt tttggaaagg cgaattatga caagatatac
      301 cttgcgttga aaaaactcgc ggaaaaaata tcacacgttt ttaaaaagat acaaagtaag
      361 aaagcagctg tcaatagaat ggaaacaaaa atggcgagac atcaatcagt ctcgccttgt
      421 tttttattgt atacattaga tatggagcag gttaccataa agatgattat tagtcgttat
      481 taaatagaat ccttcgtatt tttttgttct atcactacaa tatctttatt tggacgtttg
      541 aattgtattc cggcacgttc tagctcctgt ttcactccta catggacttt gacatcttct
      601 cgtgtcattt ttaaaacgtg atgaatcgca tagtcgattt cttgccttgt gatttttttc
      661 ttcgtattga tcatcacgac ttgcttttta tcatatgtgt ctaccaatag cagttgtgtt
      721 cgatctaacg aaaataattt gtaataatta atagccgtat tcccaaaaaa gaattccact
      781 actttaggat aaaatgtctt aatatttaaa ttactattca caggtgtttc gattagcttc
      841 atctcattcc tcttttctat atagtcgatt tagatccaat cttaagttat gttaccatct
      901 ttcctagcat taaggaatcg gttacaattg aacctttaaa gacaaaagga tttacctttt
      961 atcacttaat agtataacat aatttcgtga gaaaaaaata caattttttc ttgaccatct
     1021 cgttacgtaa aggattatgc ttacatttgt caggaggaaa aataatggag tacacaataa
     1081 agaaaatgtc agagatatca ggagtcagtc cgagaacctt gcgcttttat gatgagattg
     1141 gactgctaaa accagctagg atcaattctt caggttatcg gatttatggc aaaaaagaag
     1201 tcgatcgtct gcaacacatc ttattttatc gaacaatggc attcaagttg gatgatatcc
     1261 aagaggtctt agacaatcca tcttttgacc accagaaagc tttaatcaag catcagcaaa
     1321 tgttgctgga aaaacgggca caaatcgata ccctcttgac aactgtccaa cagacacttg
     1381 atatgtacga aggaggaaga aaaatgagtg acaaagaaaa atttgaaggg tttaaacaac
     1441 aaaagttgaa agaaaatgaa gaatcctatg gacaagagat tcgacaaaaa tacggaaaaa
     1501 aaaaggttac agcatccaat gaaaaatgga tgaatatgga taaagaaacg tatgaagaaa
     1561 tgcaaactgt tgaaaagcag atgttatttg atctcactct ttacttaaaa gaaccagaag
     1621 atgaaagctt agcagaccgt atcttccaag ctcataagaa gtggttctct tacagctggc
     1681 ctgaatatca gccaaaagcg cataaaggac taggaatgat gtacgaagcc gatgaacggt
     1741 tcactgcata ttatgacgag cgaagtggtc atggtgcagc aaaagcttta aatgaaatta
     1801 tccaaaaaaa ggcgtaatct tttacatgat ccaacaaaaa agtacacgta aaatgaccta
     1861 ataacaaaac agcagacacc caagttagat ttttatgcta acttttggtg tctgctccat
     1921 tctttcacaa gttttttcat cctactttaa atatattcat cgttgaataa acgtataagg
     1981 tattttcact ttatcctttt tttctgtaag aaacaattga aatgccattt ctccgcaagc
     2041 ttccagatgg tggtcgatgg ttgggaaacc aagaagctcg ctgatcagca aattatcgcg
     2101 tccaatcacc actggtgtct tatcttgcag atagctttgc aagatgcctg ctgcaaccat
     2161 atccgaattt gtaaagacac catcaactcc aagctcttta aaaaagaagc ctgcttgttt
     2221 tcctttctct gcatctatac aatcccaaaa aacaagtgat tcatcaaaag atggaaaaag
     2281 ttgatgacat agctgcaaag tgatttttga gttataactt agcctgccac ttcgccccaa
     2341 cgtgatgccc aattgcttta taccttgctg cttcaaataa gtaagacctt ctgtaattga
     2401 ttgttctcga tcaataaaga cacagcttgc ctgttttccc ttgatttctt cacaaaaaac
     2461 tagaggtcca tatttttgat atgccaataa atcatctagt gtattggcac gagacgtaat
     2521 gatcaatcca tcgaaagctt tagctgcaaa ttcttcaaga taccttcgct ctttttcttt
     2581 atgatagccc gtcggtaaaa gtgtcacttt gtaatcataa tcaaacgctg cttcaatgat
     2641 cccactgatc aactgatcaa aatacggatg attggtataa ggcagaatca ctccaatgct
     2701 tttcgttttt ccataactta actgtctggc atttgcatta gggatataat cgacttcttt
     2761 catgaccgca agtatttctt tccttttttc ctcatctaca tatggatgtc cattgattac
     2821 acgtgaaata gtagaaactg aataacctgt gagctttgcg atatctcgaa tgtttgccac
     2881 tgctgcttcg ccacctatac aaaaagattt tcttgtaaat agtctaccac agctggaccg
     2941 cattcaaaaa actgatacat ttttccttca tgcgaaaaac tgatccgctg cccgccccaa
     3001 tatatatctt ttgtcaaatt ttcgatttca ttcattggta tttcttttac tatattccta
     3061 ttcaaccctt gaaaactgta caacgttaat acggatccct tgaactcgac aaacgcataa
     3121 aaataattgt tcgttgtgcc atctgttatc tctacataag tattcattgc aagccctcct
     3181 tttgatactt tgagtatagt gtatgaaacg cgtctcatag caaggagttt gttttgccaa
     3241 agaaaagttt tattcctctt tcattattac caaagtctct tattttaaat tgaatacatt
     3301 ataatgatac tgtaaatata tctttattat tagtgaatg
//
LOCUS       DAZKNO010000196         3321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000196 DAZKNO010000000
VERSION     DAZKNO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.201"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(561..1601)
                     /locus_tag="ACG8MY_002472"
     CDS             complement(561..1601)
                     /locus_tag="ACG8MY_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0431628.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1950..2276)
                     /locus_tag="ACG8MY_002473"
     CDS             complement(1950..2276)
                     /locus_tag="ACG8MY_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431629.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2263..3051)
                     /locus_tag="ACG8MY_002474"
     CDS             complement(2263..3051)
                     /locus_tag="ACG8MY_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0431630.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1005 a          557 c          461 g         1298 t
ORIGIN      
        1 tccgttgcgg gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt
       61 tcctgtagga cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt
      121 tttgtatttt caattatcac aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc
      181 acaagcatat cagacaatta aatttaaaac aactaaaaat atacaaaaaa gtgtaattga
      241 atattagcag cactgcctga aaagaagtaa tatcaggctt ttcagcttgg ggaaataagt
      301 gaattacctc tcttcatgaa ttattataac tcaaaaaagc acatacgcaa acactagttc
      361 cctatttttt tgaatgaaaa ataactaaaa tatctaacga ttatgacttt aaaacctacg
      421 tttgtgattg ttttcataaa cttttaatct aaaaactccg gaataatagc tatttatttg
      481 taacatttgt aataaaatta aaatcataaa aaagttctaa tttaaaattg atcattttta
      541 tagtgatcgc tttttatcaa ctactctagc caattttccg tactaactgt tggtaattcg
      601 ttaaatgttt ctctttgttc gtttgcaagc tttgccgcaa tagctttttc gacaaaccaa
      661 ttttttacat aaacgaaaat tagattctgc aaattgttta ctttttcagt tttttgtttt
      721 tgataagctt tcagcaaagt catatataat cctttgtcag catcaattcc ataagattcc
      781 agttcttcat aaacaacaac tgtttgctct aattcttcag cttttttctt tgcattatga
      841 atagtttgga cggttttttg agcgtcttcg taagtactag aaaatgtttt tatcaagttt
      901 aatacatttt ctggtacaaa tgtatttata ctgtcctctt tcatgatgtc gacgaagtta
      961 tctagtaaaa cttgattttg taactgatca gtttcagtgt ctattatgtg tctattagtg
     1021 tctatagaat tagaatagaa tagattgtct gcgattttcg caattacggt tgagtccttg
     1081 atatgactgg attttgccgt atttgcgatt ttcgcaatta cggtggtatc attgatttct
     1141 cgcggttttg ccgtatttgc gattttcgca attacggtgg tatcagtatt gtcttcctct
     1201 tccatgattt taaagacgtc ttgattcgtt acttctggct tgcctaagta caataaatta
     1261 ggcatggatt caatcttacc gtcacgttta tttactcttc ctctcttctg tttcagaaga
     1321 cctgcttttt ctaattcctt tttgatttta gatactttac cttcgcggca ttgtaacaat
     1381 ttcattaatt cttcaactgt gaatatgaaa aatatattat catcagaatc tacccagtta
     1441 tttttgactg aataatcaaa tcgatcttta agtagcatat aagcaatttt tgcatcattt
     1501 gatagatcca tgtatttttc gttagtaaaa aataccttag gcatttgata atatttttca
     1561 cgataaattt cttttgattt gtaataatta aaatcattca tagccaatcc tctttctgta
     1621 ggatagcgtt ttgttcaaaa ctatgttaca atgaattaaa caaatatatc tatccactat
     1681 atatttttct actgctgata agtcgccaaa ctttgttaaa gcagtagtct ttttttatct
     1741 ttttttagtt taccttaaaa aataaattaa agcaaaccgc tctatttcaa ggtttaagct
     1801 catagtaact atatcacgat attacaataa tattatatca cgatatcgta atattacaat
     1861 atgaagataa gcgacttatc agctttgata ctactgattt ttataaaaaa aatatcacga
     1921 tattatgata tcgtgatatt gaatgtaaat taaattttat tttgtttctt tgctagttta
     1981 tatccttcaa tcaagtattc ttcaggtatg gactttaata gcgcatcttc tataacgtct
     2041 ggtttacttt tgtctgattc agcaatagtc gtccaatatt tgagtgctga cattagaatt
     2101 ggatttatac gaatgctatc tgctgttctt acactacttc cttgatcttt tatgacttta
     2161 cttatatttt tcttggttaa ttcgatttct tctacttctt ttgatttaaa attcttttta
     2221 tttttatttc ctagtaatct acttactgca gcttgactgc gattatcttc tgccatcaaa
     2281 atcaccaact tcttttaaat attcttctgt aaatgcttta tagtcatctt cagctgttga
     2341 gggtttatta aaattgatta gatctgatcc agcagcttgc gcttcgtcaa cggttattgt
     2401 cctacgaata taagttttga aaatttttgc accaatgact ctagaaattt cggttgctgc
     2461 agacgtcatt tctttagaaa tgttataacg cttattatac ttagtaaata gaactcctcg
     2521 tacttcaagc ttgtcgttcg tatattcttt taccgtttca atagtttcac ttaactttat
     2581 tagtcctttt aatgagaaat acgcagcttc aactggaatg ataatttcat cagaaacagt
     2641 aaatgcgttg actgtaagca ttcctaaact tggtggacaa tcaattataa tataatcata
     2701 gttgtccttg attggttgaa gtactctttt aagcctgtgt tcacttccta cttgagtaaa
     2761 ttccctctct gcagaagaaa gggctatgtc ggctggcaaa acatcaattc ctttatagtc
     2821 cagaatagca tctgtaatgt cagcctcccc ttttaatact tcataaattg taggttggtt
     2881 tgttccatct atatcgaaac ctacattatc tgtagcattt ccctgaggat caatgtccac
     2941 taaaagtaca tttttaccgt aaacaaattt caaacatgct gctaaattaa cgctagtcgt
     3001 agttttagaa actccacctt tttgattggc tactgacaat attcttgcca ttaaaatatc
     3061 ccccattcat cttatattat aatataatta tatcataata ttgtgatatt gcaatatcgt
     3121 atatgcatta tattgtaata ttgtgtgatt attttgtttt aaagatatat atcggttcgg
     3181 atataaagat tattcatatg ttattcgaaa ttaaaataat cactttttat tactttaaaa
     3241 ttttttatta atgataagaa atatgttttg cgagcagcaa ctagaataat ttaaacatta
     3301 ctaaactgtc tatattgtta c
//
LOCUS       DAZKNO010000197         3309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000197 DAZKNO010000000
VERSION     DAZKNO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.202"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..894
                     /locus_tag="ACG8MY_002475"
     CDS             4..894
                     /locus_tag="ACG8MY_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0431631.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(967..1791)
                     /gene="nadE"
                     /locus_tag="ACG8MY_002476"
     CDS             complement(967..1791)
                     /gene="nadE"
                     /locus_tag="ACG8MY_002476"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0431632.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1803..3275)
                     /locus_tag="ACG8MY_002477"
     CDS             complement(1803..3275)
                     /locus_tag="ACG8MY_002477"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0431633.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT          921 a          703 c          604 g         1081 t
ORIGIN      
        1 tatatgcaac tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa
       61 acaaggatca agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca
      121 aaagaacttg gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca
      181 aacatcatca aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa
      241 atcgatatca gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata
      301 cctattggtc actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat
      361 gattttatcg ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc
      421 cgcattctct ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca
      481 gaggatacat tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca
      541 aatgacaatt ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg
      601 acgagccctg gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat
      661 aatcttaatc aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg
      721 aatggagaac ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg
      781 catttgcgaa tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt
      841 aaaggattcg gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt
      901 tcttttctat tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct
      961 ttattgttat ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt
     1021 cttgttccac catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag
     1081 ataatcatca atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt
     1141 cccatcttca agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc
     1201 ttgaagcaat tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc
     1261 accatcacca aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa
     1321 tacggctcct gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt
     1381 gatatttcct ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg
     1441 tgcatcaaca gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag
     1501 agcagctttt gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa
     1561 ttgatagttt tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg
     1621 gcctgctaaa gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg
     1681 atattttttc aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc
     1741 aattgttggt ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc
     1801 tcctatgctt ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt
     1861 ttatggttcc agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt
     1921 ttatactctt cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt
     1981 gcaggtaatt cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct
     2041 gtaaagtctc gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct
     2101 tcttcttctc gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca
     2161 aaatttcgag tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg
     2221 cttgataact tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg
     2281 aatactgcac caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat
     2341 acatcaattt tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc
     2401 gaagcataga tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta
     2461 gatatatagg ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct
     2521 aattctttcg ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg
     2581 actaagaaca cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt
     2641 ccataagatt ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc
     2701 ccagcacgta cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag
     2761 actgctgcat ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg
     2821 atgacagatt tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc
     2881 aacagtgctg tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt
     2941 tcatttgcaa aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa
     3001 ttttgtaaat actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata
     3061 tcggaatcat tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa
     3121 accgcgtatc cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa
     3181 tcagctcgac ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc
     3241 agcgtcaaac tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt
     3301 ttcatttcc
//
LOCUS       DAZKNO010000198         3301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000198 DAZKNO010000000
VERSION     DAZKNO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.203"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..1983
                     /locus_tag="ACG8MY_002478"
     CDS             55..1983
                     /locus_tag="ACG8MY_002478"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0431634.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2121..2576
                     /locus_tag="ACG8MY_002479"
     CDS             2121..2576
                     /locus_tag="ACG8MY_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0431635.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            2656..2784
                     /locus_tag="ACG8MY_002480"
     CDS             2656..2784
                     /locus_tag="ACG8MY_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431636.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            3046..>3301
                     /locus_tag="ACG8MY_002481"
     CDS             3046..>3301
                     /locus_tag="ACG8MY_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431637.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKII"
BASE COUNT         1005 a          588 c          655 g         1053 t
ORIGIN      
        1 atcaattaca tttttattat gctatccttg ttttgacaag gaggtctaat aacgatgact
       61 aatcagaaaa aagcttttgc agcaacaatc ggctgccttt tatttatgat cttcggtttt
      121 atcttcgacc gattctctct taccatcacc ccactttttt atatcagtgc cattatcatc
      181 ggtggatata aacaaacatc ggaaggcttg aaagaattgt ggaatgatcg gacactgaat
      241 gtggatttat tgatggcttt agctgctatt ggtgcctgtt tgatcggtaa ttactttgaa
      301 ggagcaatgt tgacattcat cttttgttta agcggtgcat tagaggaata cacaactaat
      361 aaaagtcaaa aagaaattac tgctttgatg aatcttcaac cacaaaaagc ccaattatta
      421 aaagaaaatg gacaaatgca agaagtagat gtttctcaac taaaggtgga taacttggta
      481 tttgtcgcta aaggagcagc tgttccgata gatggctttt tagaaagcac acaggcaacg
      541 atcgatgagt ctgcattaag cggtgaatct gtgcctgtag aaaaatattt aggcgatgag
      601 atgtatgcag gaacgctgaa tcaaggaaat cccatcacga ttcgagtaac aaaaacgagt
      661 tccgaaactg tctttgctaa gattatccaa ttagtagaag aagcgcagaa tacgccaact
      721 caaacagctt catttatcga acgaatcgaa aataattatg taaaattgat tttgcttgct
      781 gttcctctaa tgatccttct cccccacttt ttcctaggtt ggagttggga tgaaagcttt
      841 tatcgaggaa tggtattatt agttgtggct tctccttgtg cgcttgtcgc atctgctaca
      901 ccagcttctc tagctgctct ttcaaattct gcaaaaaatg ggattttaat caaaggcggt
      961 atccatttag aaaagctttc tgaattaaaa gcgattgcct ttgataaaac aggaacgctc
     1021 actaaaggaa aaccagttgt aactgacagc tttttctttg atgaaaagga tttggcacaa
     1081 caacttcttg ttgctatgga acaaaaaacg acgcatccac ttgctcatgc gattttgcaa
     1141 tatttcgatt gcacaatacc tgaagaaatc caatacttac caatagaaga aattactggt
     1201 tttggattac aaactaccta tttaggtgcg caatggaaag ttggcaagca cgcatatgat
     1261 cctgagacga tgataatcag caaagagatt gcggaaatga tcgaacgttt ggaaaatcaa
     1321 ggcaaaaccg tgatttatct ttctaaagat cagcagttaa ttgctgtttt aggtctgcta
     1381 gatattccaa aagcaaatac ccaacaagta atttcctact ttaaatccca aaacatccat
     1441 acaagtatga taaccggcga ccattctggc acagcaaagg ccattgctga acaagtaggg
     1501 attgaacatt actatgcaag ctgtactccg gaagaaaaaa cacaactagt caaaaaagag
     1561 aatgaacttt accaagtaaa tgcgatggtt ggtgatggag tcaatgatgc gcctgcttta
     1621 gcagccgctt ctttaggtgt cgctatgggg caagggacag atgccgctat ggatatcgca
     1681 gacatcgtct taatgaaaag cgacttaaat aaatttgtct acagtcatcg gctatcttta
     1741 aaaatgcggc ggatcatcaa acaaaatatt attttttcga ttcttgttat tctcttgttg
     1801 attgcttcta acttcctgca atttttaaat ctacctcttg gagttgtagg acacgaagga
     1861 agcacaatat tggttattct gaatggcttg cgtttactaa gacctttacc tgatctttct
     1921 gattcttcaa aaaaatgtaa gtcttgtcca ctatacaaag cgcggctgtc ttctgtcaaa
     1981 taagcaaagt gtttttttat gattttgttt attttatgaa aaaaacagct tatgaatagg
     2041 tcttgaagga tgaattccta cttttgtaac tttctaaaaa catgttatga ttgtttcgaa
     2101 gaaagtgagg aatatatttc atggaaagtt ggcttttttt aggaattatt ttacttgtag
     2161 caattttagc taaaaatcaa agtctgatga tagcggcagc ttttgttttg attatgaagc
     2221 tcataccagc ctcccgttcc tttttccctt ggcttcaagg caaaggaatc aatattggag
     2281 tgacgatcat cacagcggct attctaatcc ctatcgcaac aggagaaatc ggtctacgtg
     2341 atctattaga agcctttaaa tcccctattg gctgggtagc tgttttttgc ggtgggctag
     2401 tggctgtctt atcttccaag ggtgtaggat tgattgcttc tgatccacaa atcactgttg
     2461 cacttgtttt cggcacgatt ttaggtgttg tcttcttaag aggaatcgcc gccggaccgg
     2521 tcattgcttc tggtatcact tactgtatct tacaagtatt tgctttagga aaatagaaca
     2581 aattttgtct attttaggag attcttaaca cccccaattt actgatccgt gatatactga
     2641 caacgaggtg atacaatgtt cgaaaaatta aagtatttca taggaacaat cggctggttt
     2701 cttctagcgg tacttttgat tctgggctta gcttatctat tatttcctga tttgatacaa
     2761 aatgtgtttg atactgcttt ttgattgacc tattaaaacg atatagggga tgctcctgcg
     2821 atgcaggggc tttttttaat caatgttttg taataattta ggccgtgcca atgagacttc
     2881 ctatagaatt cgatgctctt ttttcttttt ggctcttctg ctcttttttg ttatactaga
     2941 ttacagtgga caaaagaaag aatgatcgca tccacttcaa cgaatatcaa aatggaagaa
     3001 ttagtgcctg ttttcaggtt actttgaaaa gaaaggaaga cgcttgtgga aacttttgaa
     3061 ggtcttttaa ttgatacgta cgttttacaa gaacagccaa tgatgctgac tgggatcaac
     3121 ttgcagcttg aggaccgtgt gatcaatggc ctgcttaaag atcgctattt ggcaaaatat
     3181 atcttacgtt tgcccctaaa taaatatgtg ctggaagtaa gtggcaaatg gaaccgtcgg
     3241 aaccagttgg tcataaaaaa aatgttgata aaaaatatcg atgaatacat taaaataatc
     3301 g
//
LOCUS       DAZKNO010000199         3286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000199 DAZKNO010000000
VERSION     DAZKNO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.204"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            418..1155
                     /locus_tag="ACG8MY_002482"
                     /pseudo
     CDS             418..1155
                     /locus_tag="ACG8MY_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1335..1940
                     /gene="fic"
                     /locus_tag="ACG8MY_002483"
     CDS             1335..1940
                     /gene="fic"
                     /locus_tag="ACG8MY_002483"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0431638.1"
                     /translation="MILENKLGLTNQVELAKAEEKLSKQKAKELYDSGKINEIEVGTF
                     KGLSEIHDFLFSEIYDFAGKIRTVNIAKGNFRFAPVMYLEQSLNHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1956..2531
                     /locus_tag="ACG8MY_002484"
     CDS             1956..2531
                     /locus_tag="ACG8MY_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0431639.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMDRDMIVERTQEGKLFAKKNNPNFKEGRPKATITPKKRHAYELI
                     TSGKSYKEVEAITGFSRSTLFRIKKQIEASE"
     gene            2691..2894
                     /locus_tag="ACG8MY_002485"
     CDS             2691..2894
                     /locus_tag="ACG8MY_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0431640.1"
                     /translation="MKEQQLGYTLWGRPLYGLERRTAVSIDCACGELIQLNIQTKQYN
                     CKNCGNCYQFIDQDFQLLNEKNK"
     gene            3003..>3286
                     /locus_tag="ACG8MY_002486"
     CDS             3003..>3286
                     /locus_tag="ACG8MY_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFQ0431641.1"
                     /translation="MLDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKR"
BASE COUNT         1149 a          536 c          652 g          949 t
ORIGIN      
        1 aaataaagta gtaaattaat cataatcttt tcaacaacag gaatagtcaa gttagactgt
       61 tcctgttgtt tctttatttt ccttatcatc ttcaactttt tcaaatactg caaatacaaa
      121 atatcaatat aacttattcg ttctatcaat cagttcctaa tatctactca tcaaaatgaa
      181 atttcaaact aatctcatca tagtattctt ttcgtcgatt gtaaaattaa gctagacaac
      241 taaaaagatc atttgtgcta cactcaaaat agttccgacc aaagaattat taaggagtga
      301 gtacaaatga cctacaccca tcttacaaca gacgaactgg taatcataga gtcttatttc
      361 aaaaataatc aatccgttgc caaaactgcg caatgcttag gacgtgcgtg acaaacgatt
      421 cataacgtgt atcaattttt gaaaactggt gctacgatac tgaactacta tcaacaatac
      481 aaagaaaaca aaaaacgctg cggtagacgc tcgattgctt taccagagga acaaaaagaa
      541 tatgttcaaa agagggttgt tcaagggtgg acaccggatg tgattgtcgg ccgctcggaa
      601 atgaaacttg attgttcagt ccgtacactg tatcgaatgt ttcaaactgg cgcatttaat
      661 gtgcgtgatt tgcctatgaa aggcaaacga aaaccaaatg gacaccaaga aaaacgtggg
      721 aaacaagctt ttcggcgcag tatacatgag cgcatcaagg agcatccaga atttgaaaac
      781 gagtttggcc atcttgaagg agacactatt gtcggtcgaa aacacaaaag tgctgttatt
      841 acattggtgg aacgtttgtc aaaagtgatt attacattga agccagccgg tagacaggca
      901 atggacattg aaaatcgaat taatcattgg tttcaaacgg ttcctaaaca tctgtttaaa
      961 tcaattacgt ttgactgtgg gaaagaattt tcaaattgga aagctatggg aaatcaaaat
     1021 gatattgata tttattttgc ggatccaggc accccgtcac aacgcgctct aaatgaacat
     1081 tcaaatggat tattacgtcg tgatggatta gttaaagcca tggacttcaa tgaagtatca
     1141 gaatcattta tagagtacag aaaacatttt ctgtactcta ttttgtcttc ttttaaattt
     1201 tcaatagatt atatctattg tgaattttgt agagggtcag aaacttaata gtttttgaca
     1261 catggttaaa ttcgcgatac aataggattt gtagtgtttc ataaacttat gtcaaaaagt
     1321 taaggggatt ttccatgatt ttagaaaata aattgggttt aacgaatcag gttgagttgg
     1381 ccaaggctga agaaaaactc agtaagcaaa aagcaaaaga attatatgat tctggaaaaa
     1441 taaatgaaat cgaagtcggc acgtttaaag gcttgtctga aattcatgat tttctctttt
     1501 cagaaattta tgattttgca ggaaaaattc ggacagttaa tattgcaaaa ggaaattttc
     1561 gttttgcgcc ggtcatgtac ctagaacaat ctttaaatca tattgatcag atgccgcaaa
     1621 cttctttcga agagattatt aagaaatacg tggaaatgaa tatcgctcac ccttttcgcg
     1681 aaggaaacgg gcgcagtact agaatttggc tggatctcat tttaaaggaa gaattgcaaa
     1741 aagtggtcga ttggaacttg atcgataaag cagactatct ttctgcaatg gagcgcagtc
     1801 caataaatga cttggaaata cgctatctca tttccaatgc cctgactgat aaaattgacg
     1861 atcgtgagtt atatatgaaa ggaatcgatg tcagctactt ctatgaagga tactctgagt
     1921 atacgataga tgatttgtaa aagcaggtga gttaaatgac aaaatatggc tatgcacgtg
     1981 tctcaaccgc ttctcaaagt acaaaggaac agatacaaca gctacttgag aacggtgttg
     2041 agaagaagaa tattttttct gaaaaattta caggtacgaa acgagatcgg ccaaagttta
     2101 atcagctgtt ggctggtctg aagcctggag acgaaatcgt tgttacgaaa cttgatcgat
     2161 tcgcccgtaa tacaagagaa gcactagaga tcattgaacc tttactcgat gataacgtca
     2221 cgattaaggt attgaatcta gggacgatcg aaaatacatc gatgggtcga atggttacac
     2281 gtacgttgtt gagtgtagct gaaatggacc gtgatatgat tgtagaacgt actcaggaag
     2341 ggaaattatt tgcgaaaaag aataatccta attttaaaga gggacgtccg aaagcaacaa
     2401 tcacaccaaa gaagaggcat gcatatgaat tgattacttc cggcaaatcg tataaagaag
     2461 tggaagccat aacgggtttc agtcgtagta cactttttag aattaagaaa caaatagaag
     2521 cgagtgagta gaagattgat aaaattaata taaaaatctc ttttaaatca aataaatcgt
     2581 tgtttatacg agcattctaa ggtcaggttc actcattaag atgtatcatt ggtcgaacga
     2641 agcgtcatac cagctacgaa tagacatatt tttagggagg aacgaagtaa atgaaagaac
     2701 aacaattagg ctacactctt tggggacgtc cattatacgg attagaacga agaacggctg
     2761 tatcgattga ctgtgcttgt ggtgagttga ttcaactcaa tattcaaacg aaacaataca
     2821 attgtaaaaa ttgtgggaat tgttaccaat tcattgatca agacttccaa ttgctaaacg
     2881 aaaaaaacaa ataggtttat tatagtcaga caattgtctg gctatttttc tatttagaga
     2941 agatcctcgt tgcaaacacg aacgtatgtt tgtataatat gagagaagag ggggagaact
     3001 ttatgcttga ttactcaaaa gagccagtca atgactactt tctaatcgat atgaaaagtt
     3061 tttacgcaag tgtcgaatgt atagaaagaa atttagatcc attaacgact gaattagttg
     3121 tcatgagtcg agcggataat acaggatcag ggttgatttt agcttcttca ccagctgcaa
     3181 agaagaaata tggtatttct aatgtcagca gaccacggga tctaccacag cctttcccta
     3241 aaacattaca tgttgttcca ccacgtatga acctttatat taagcg
//
LOCUS       DAZKNO010000200         3268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000200 DAZKNO010000000
VERSION     DAZKNO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.205"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG8MY_002487"
     CDS             complement(<1..2232)
                     /locus_tag="ACG8MY_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0431642.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3268)
                     /locus_tag="ACG8MY_002488"
                     /pseudo
     CDS             complement(2904..3268)
                     /locus_tag="ACG8MY_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          896 a          728 c          535 g         1109 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattt
//
LOCUS       DAZKNO010000201         3232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000201 DAZKNO010000000
VERSION     DAZKNO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.206"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..999
                     /locus_tag="ACG8MY_002489"
     CDS             139..999
                     /locus_tag="ACG8MY_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFQ0431643.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            1011..1838
                     /gene="panB"
                     /locus_tag="ACG8MY_002490"
     CDS             1011..1838
                     /gene="panB"
                     /locus_tag="ACG8MY_002490"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFQ0431644.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1853..2701
                     /gene="panC"
                     /locus_tag="ACG8MY_002491"
     CDS             1853..2701
                     /gene="panC"
                     /locus_tag="ACG8MY_002491"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFQ0431645.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2711..3106
                     /gene="panD"
                     /locus_tag="ACG8MY_002492"
     CDS             2711..3106
                     /gene="panD"
                     /locus_tag="ACG8MY_002492"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFQ0431646.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT         1253 a          329 c          644 g         1006 t
ORIGIN      
        1 ctatctttta ttttaaaata tgggatatta tgataaatta actatggatg atatttgaaa
       61 aaaccatact gtatatttga tttaattttt atttttaagc tcatatcgta agaatgggac
      121 ttgtagtctc acttctttat ggaggtgata attatgaacg ttggttttat tggagccggc
      181 aaggtaggtt gttcattcgg gaagtatttc caggaacata aaatacaagt aacaggattt
      241 tatagtaaaa gcgaagactc gtcattagca gcttcgaatt ttacatctag taaacaatat
      301 ttaaacttaa gagaattagt agatgaaaat gatactattt tcattacgac tcctgatggt
      361 caaattcagg aggtatggca agaaattaaa aattatcaaa tcaaaaacaa attaatctgc
      421 cattgcagtg gtgcaatatc ttcagatatc ttttcaaata tacaggacta tggtgcttat
      481 ggctattctg tccatcctat gttttcaata tcagataaat ataattcata caaaaaatta
      541 aaaaaatctt ttataacaat tgaaggcgat gaaaaatatg caaagttttt atgtgatttc
      601 ttcaaaaagt taggtaatag tactgtaatt gtatctaagg agaataaatc tctttatcat
      661 gccgcatcag tagtatctag taaccttgtg ctaggactta ttaatacaag cgttacatat
      721 ctaattcaat gtggatttac tgaaaaaatg gcaattgaag ctttatatcc attaattgaa
      781 tttaatatta gaaatataaa agaaaaagga attatagaaa gtttgacagg gccagttgaa
      841 cgttgtgata tttcaacaat taaaggtcac tgtgaagttt tatcagatga agacagagct
      901 ttatatatgc tattatcaaa aaatgtatta gagatagcta tgttaaaaaa tattaataga
      961 gattatagtg aattagaaaa gtatttagga gagagttagg agagaaatag atgaaaaata
     1021 cagcagttac atttaaagaa tctaaattaa gaaatgaaaa attaacaatg cttacagctt
     1081 atgactattc aacagcaaaa ataattgatg aagctgggat aaatggaata ttagtaggag
     1141 attctcttgg tatggtatgt ctaggacatg aagatacact ttcagtaaca atggaagaca
     1201 tgattcatca tactagagca gttacaagag gagctaagaa cactttagtt gttgcagata
     1261 tgccatttat gtcttatcaa acttcagtat atgattcagt agttaatgca ggaagattaa
     1321 taaaagaagg acgagctcag gttgttaaat tagaaggagg gatagaagta tgcgataaaa
     1381 tagaggctat agtaaaggct tctatacctg ttatggcgca tataggttta acacctcaat
     1441 ctgttaatgc atttggagga tttaaggtac aaggaaagga caaggaagct gctaaagaat
     1501 taataagagc tgctaaagct gtagaaaaag caggagcatt cgctgtagtg ttagaatgtg
     1561 ttccaactaa gcttgcagag ttaatttcta aagagatatc aatacctaca attggtattg
     1621 gagcaggtgc agggtgtgat ggacaaattc ttgtatatca agatatgttg gggatgtata
     1681 gtgattttac ccctaaattt gtgaagaaat atgctaattt atcagaagag atgaataaag
     1741 catttacaaa atatatagag gaagtaaaag atggagtttt tccaggtcca gaacatgggt
     1801 ttaaaataag tgatgatgtt ttagaaaaat tatattaaaa ggggcaagaa atatggttat
     1861 atttaaaaca gtaaatgatg ttagaagtca agtaaaagag tggaaaaaac aaggcttaaa
     1921 ggtaggatta gttcctacta tgggatattt acatgaaggt catgaaagtt taattagaaa
     1981 agcatcaaaa gaaaatgata aagttgttgt aagtatcttt gtaaatccaa ctcagtttgg
     2041 gaaaaatgaa gatttaggta gttatccaag agacttagaa agagatatag aagtatgcac
     2101 aagaggtaga gcaacagcaa tatttaatcc tgaagttgaa gagatgtatt gtgacaatgc
     2161 aagtactttt gttaatataa ctggattaac agaaggttta tgtggagcta gtaggccaat
     2221 acattttaga ggggtttgca ctgttgtaag taaattattt aacataatac cagcagatag
     2281 agcttatttt ggagaaaagg atgcacaaca gttagctgta attaaaagaa tggttaggga
     2341 ccttaatatt gatatagacg ttgtagggtg tccaattata agggaagagg atggactagc
     2401 taaaagttct agaaacacat acttaagttt agaggaaaga tcttcagcta ctattttaaa
     2461 taaatcttta actttagcta aggaagcttt aaataatgga gagagagaca gtttaaaaat
     2521 aattgagata ataagtaaaa atataaatac ttataatttg gcaaagattg attatgtaga
     2581 agttgttgat tctttatcat tacaaagagt aaattatatt gaaaaatcag ttttagttgc
     2641 aatagcagta tttataggaa aaacaagatt aatagataat tttacatttg aattacaata
     2701 gggggctata atgaaagtta caatgttaaa aggaaaaata catagagcta cagttgaaca
     2761 agctgagtta gattatgtag gaagtattac tgttgatatg aatttattag aagcagcagg
     2821 aatatatgaa tatgaaaaag tacaaatagt tgatgttaat aatggaaata gatttgaaac
     2881 ttatacaata gctggagaga gaggaacagg gatgatttgc ttgaatggag ctgcagctag
     2941 atgtgtatca actggagata aaataatttt aatggcatat tgtgaattaa atgaaaatga
     3001 ggttaaggat catagaccta aggttgtttt tgtcgatgat aataataaag ttgaaagagt
     3061 aacatcatat gaaaaacatg gaaggttatc agatataatt ctttaggttt tgtaatgtta
     3121 tttatatata gctagactat tattgaaata tttattgatt actaattgga tagatgtcaa
     3181 taaagtagac acaaaaagag aagatacaga ctgtagacaa agtccatttt at
//
LOCUS       DAZKNO010000202         3171 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000202 DAZKNO010000000
VERSION     DAZKNO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.207"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..445
                     /locus_tag="ACG8MY_002493"
     CDS             <1..445
                     /locus_tag="ACG8MY_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0431647.1"
                     /translation="HKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNF
                     FKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDF
                     REVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(531..1625)
                     /locus_tag="ACG8MY_002494"
     CDS             complement(531..1625)
                     /locus_tag="ACG8MY_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0431648.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1814..2575
                     /locus_tag="ACG8MY_002495"
     CDS             1814..2575
                     /locus_tag="ACG8MY_002495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0431649.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2690..3130)
                     /gene="fabZ"
                     /locus_tag="ACG8MY_002496"
     CDS             complement(2690..3130)
                     /gene="fabZ"
                     /locus_tag="ACG8MY_002496"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0431650.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT         1015 a          631 c          534 g          991 t
ORIGIN      
        1 tcataaaagt gccgtcatta ctttagttga aagattatct aaagtcatta tcacgattaa
       61 acccaacggc cgtaaggcat tagatattga aactgccctt aatcaatggt tttctcgctt
      121 ccctaaaaac ttctttaaat ctattacgtt tgactgtgga aaagaatttt ctaactggaa
      181 agccattagt aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca
      241 acgcccatta aacgagaatt ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat
      301 ggattttaga gaagtgaatc agacatttat ttccagtgtc agcaatcaac gtaatcatat
      361 tccaagaaaa tcattgaatt acagaacacc aatcgaaata tttttgagct atgtacaaga
      421 agcattttat tctagcttaa tttgacaaat cataacctat aaaaaaacaa aataaaaaag
      481 cagaaataac tggctatttc tagcggatta tttctgcaat aactaattat ctatttttta
      541 ttttcaatta atttaaagct tgaccatggt ttcaaaaagt caaggaggaa taattcgtca
      601 tcggagattc ttcctacaac attgacacgt ccgtcgtttt tcatatcctt caaggcgatt
      661 tgtgtttctc ctttgtattg accgtaccct tcattgtcaa tcagtacatc cccatgatgc
      721 atatctcgcg tattatgagg cgggaaatct ttatctttgt agtatacacg agtcatcgtt
      781 gaacgaagga tatattctga gcgatcccct cgataactat gaaggttatc aaacaagcag
      841 attcgttcgt tttctgtaat accttcttcc gtatctactt tcaatgttgg ataatccgcg
      901 ttaaatgctt ctgccatttc tttcaattct tcttcacttg cgtacgcatt tccaatagaa
      961 atatcatcaa tcaatcctgt aaggattaaa tgcttcactt gtgtcgcaat ttcaagatct
     1021 cggtggtctt ccagcgtgca caagccatct tgcgtaggcc aagggccaaa ctcagcagat
     1081 tgcgaattga cgaatgccat cgtattcaaa ttatattttc tgaatttttc tgaacaaaaa
     1141 acaaagtgtt cgtatcctaa accagagtat cgatgaggat aaaaattatg agaacctaat
     1201 aaattatccg tgtttggcga ataactcatg atgttgtcaa cgtaattcgt gcccgaactc
     1261 atattgatct cgattttgat accaaaagga ttgcgcgtca tttttgcttc ttctgctcct
     1321 gtgaatccga tatctaagcg gaccccgtag gcacccatct tatggaaaaa agataagtca
     1381 tcgtaagaaa tctccaactg ttcaaataac gctggattga tatcaaccat tacttccatg
     1441 cctaggctat tcgcgtaatc aactacttct ttaaattctg aaagaacttt ttctttatca
     1501 tcattgatct gcagcaaact ggtaaaaacc cgtttatacc catacttatg agctaaatcc
     1561 aagtacgctt tgtccttttc aaatgttgac cgttctggat aaatcgagat tcctaatttt
     1621 cccatattac catttcctcc tgcatctttt cgtttttgta tccacttaca ttatagcaaa
     1681 cacaaagtaa atggtctaca ccttttcttt ttcttttaaa ataaatgata aattccaata
     1741 atttaagtag gtattttccc tctattttgt tatcattaaa gataacaaaa tagtaaattg
     1801 gaaaggaaca gttatgaaga aaaataaagt tattttaagt attagtttag cagcagcatt
     1861 gctgtttgct gctggttgtt ctgcaaacaa cacgacttct tctagttcgt ctacagaaag
     1921 ttcaaaaagt acatctactg cggaaagcac gactgccagt agcgtcgatt tgaacgcatt
     1981 ggaattacca caattgacga aagatgtcgc cgaaaatgaa gatctggtcg aaatggtgac
     2041 gaataaaggc acaatcaaaa tcaaattatt tcccgaacac gcaccaaaag cggttgaaaa
     2101 ctttatgaca catgcaaaag acggttacta caacggactg actttccatc gcgtcatcaa
     2161 agatttcatg atccaaggtg gcgatcctaa aggcgatggc actggagggg aaagcatttg
     2221 gggagaagga tttgaaacag agatcaacaa ccacctttat aatataagag gtgcgttatc
     2281 tatggctcgc tcacaagatc ctaacagtaa cggcagccag ttctttatcg ttcaaaatac
     2341 agatgatatg catgatggtc ttttaaaaga cgactatcct caagcaatca tcgacgctta
     2401 taaaaacggc ggttatcctt cactagatgg ccattatact gtcttcggcc aagtgattga
     2461 aggaatggat gtcgtggatg ctattgcaaa tatcgaaaca gatagttcag acaaaccaaa
     2521 agaagaagta aaaatcgata aaatcaatat actgcaagaa gcaaaacaaa aataaaaaaa
     2581 taacgtctga atcctgcata gatgacaacg tatgctagga ttcaggcgtc ttctttttac
     2641 ataaaaagca agtaagcttc cttttgtttt gctatatcag gttttatagt caagcatttt
     2701 ggttgacgat gaacgtgaac tcgcactcac agactttttt atcatctaca tatgctgcag
     2761 ataacgctgt accgactgat cccttcattt tggtgatttc aaaatgacat ttcagcacat
     2821 ctcctggaac aactttttga cggaatttgg ccttattgat cccaccgata taagctcttt
     2881 ttcctagaaa ctcttctgat tttaggataa gtatcgaacc tgcttgtgcc agtgtttcta
     2941 gaatcaacgc acccggcatc actggattgt caggaaagtg accttggaaa aaatcttcat
     3001 tgattgttac attttttgtc gcaacgatct tttttcctgg ttctaaagta tctacataat
     3061 caatataaca aataggataa cgattaggaa tcaattccat tacttcagta gcagataata
     3121 cgtaagacat aattaaactc cttctttcag gcaattgcca ttttcattac t
//
LOCUS       DAZKNO010000203         3157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000203 DAZKNO010000000
VERSION     DAZKNO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.208"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..410)
                     /locus_tag="ACG8MY_002497"
     CDS             complement(<1..410)
                     /locus_tag="ACG8MY_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431651.1"
                     /translation="MKHMRHIAKEVDDWTRVEAEFSGDYAHQLTDAIKECSTDEQLKN
                     LIISSLLDRYMFFYVNSNRPHKITRLMLELLDEKNFHFESPSPRNNLLEQSIDHLIKG
                     SGLLPTLWKVQQIWGGDTAKELIDYLYKQYYEEF"
     gene            complement(407..601)
                     /locus_tag="ACG8MY_002498"
     CDS             complement(407..601)
                     /locus_tag="ACG8MY_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431652.1"
                     /translation="MASTNKSYLQKVIDDNKEKPYKRNNSKVTDVSKDFQAQTLYIGA
                     RSRNTKVHLRIYDKKEEQLS"
     gene            complement(588..1079)
                     /locus_tag="ACG8MY_002499"
     CDS             complement(588..1079)
                     /locus_tag="ACG8MY_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431653.1"
                     /translation="MIKPDIDQFTLVLQTTGVFDFDEWRDWVAKSLISTFLIKSKMHK
                     LFDNFGEADVKLPEGYTIGYSFINAPFYFCIAYHEAFSKMGVIVKYSAYAWHEYRKRY
                     EAEFNEPIHLHTFLKMIDSDEYSFRLSRIDICYDFINEGIDIAKLKRSIEEKRTELRY
                     GKY"
     gene            complement(1308..2609)
                     /locus_tag="ACG8MY_002500"
     CDS             complement(1308..2609)
                     /locus_tag="ACG8MY_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0431654.1"
                     /translation="MKKTYRMQRGFISFLFIGFFLILWFFFSKLYPLIVEQITIIQLP
                     HIESNIFLFPVGLVVVGLFVSWSIRNKLWFGLFFSVHYYLMIKRIRRHVKDARFEDER
                     EFDNRLVKLPIIKIEFDDNRARKSGKVFIQNSIKFDKKLEDMRIDAALEGYVSERQYL
                     SKDRNWYIYEFYDVDSQKQLEIKSASDLIEWSNKKTDDYSLRLDERATVPFHHMGLVG
                     QTGSGKSFFIQMLVEQVLSKKVSHELFVIDPKRTDVYQMAKRTVGDERTADKTNAIEL
                     INQFHERMKKRQDELQDYFISNRNKTYRDAGLPALILLIDEFGALRESWKTLAKKERD
                     EVDSILSDVAFMGRQLGCILWVATQQMNAQTMPTAIREQLVLKIALGDSDEQTYRTLF
                     SSGVDIPPVQFTAGQGIYSYPGLASVDKPRMLAVPYCSYLE"
     gene            complement(2721..3068)
                     /locus_tag="ACG8MY_002501"
     CDS             complement(2721..3068)
                     /locus_tag="ACG8MY_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431655.1"
                     /translation="MKKLRQFNKFDAEVFFKDKDVRVMAHEPWNEYEDGQVTKELGTK
                     YKCVIATDNTKYEGNDVQPDLNAGEQIDVKVTQPPKSYKKFSRITFVNPTASVYGTFM
                     NELSVKADDVDIV"
BASE COUNT          936 a          654 c          504 g         1063 t
ORIGIN      
        1 tcgaattctt catagtactg tttatataaa taatcaatta attcttttgc tgtgtcgcct
       61 ccccatattt gttgaacttt ccacaatgtg gggagtagac cagacccctt aattaaatgg
      121 tctatcgact gctctaataa gttatttcgt ggagatggtg attcaaagtg aaagttcttt
      181 tcatctaaca attcaagcat taatctagtt atcttatgag gtcgattaga attaacataa
      241 aagaacatgt agcgatcaag tagagaacta ataatcaaat ttttaagctg ttcatctgta
      301 ctacattctt taattgcatc tgttaattga tgggcgtaat cacctgaaaa ctctgcttca
      361 acacgtgtcc agtcatccac ttctttagcg atatggcgca tgtgtttcat gacagttgtt
      421 cctctttttt atcataaatg cgtaaatgca cttttgtatt tctgcttctt gccccgatat
      481 acaatgtttg tgcttgaaag tctttggata cgtcggtcac tttcgagttg tttcttttgt
      541 atggtttttc tttgttgtca tcaataactt tttgtagata acttttatta gtacttgcca
      601 tatcgcaatt cagtcctttt ttcttcaatt gagcgtttta atttagcaat atcaatacct
      661 tcattaataa agtcataaca gatatcaatt cgtgaaagtc taaaactata ttcatccgaa
      721 tcaatcattt ttaaaaaggt gtgtaaatga ataggttcat taaattctgc ttcatatcgc
      781 tttcgatact catgccatgc atatgcacta tatttgacaa tcacacccat tttcgaaaat
      841 gcttcatgat aagcgataca gaaataaaaa ggtgcattga tgaatgagta acctattgtg
      901 tagccttctg gtagcttaac gtcagcttca ccgaagttgt caaatagctt atgcatctta
      961 gatttaatca agaaagtact tattaggctt tttgctaccc agtcacgcca ttcatcaaaa
     1021 tcaaacacgc ctgttgtttg taatacgagt gtaaattggt caatatctgg cttaatcatg
     1081 ttctattcgc tccttttcct aagaaaactt taggtaaagt ttacttggtg taaaagccta
     1141 taagaatagg ctttagtctg ggttttactc ctgactggag ctggggtgat taccaatccc
     1201 ccagctctcc taaaggaggg aagcacggag cggaaccttt ccctgcctaa cggcgggtcc
     1261 ctttccgctc attgtgcaac cgttccttaa gaaaaagcta agtgctatta ttcaagatag
     1321 ctacagtatg ggacagcaag cattctcggt ttatcaacac ttgctagccc tggataactg
     1381 taaatccctt gaccagcagt aaattgaact ggaggtatat ccacaccaga agaaaacaga
     1441 gttctatagg tttgctcatc actatcacct aaagcaatct tcaaaacgag ctgttctcta
     1501 atcgctgttg gcatcgtctg tgcgttcatt tgttgcgtag cgacccacag aatacaccct
     1561 agttgacgac ccatgaaagc aacatcagat agtatgctat cgacttcatc acgttctttt
     1621 ttcgctaaag tcttccatga ttctcttaat gccccgaatt catctataag caaaattaaa
     1681 gccggcaaac cagcatccct atacgttttg ttacgatttg atatgaaata gtcttgtaat
     1741 tcatcttgtc gttttttcat acgctcatga aattgattaa tcaattcaat cgcatttgtt
     1801 ttatcggctg ttcgctcatc accaacggta cgtttagcca tttggtaaac gtcagtacgc
     1861 tttggatcaa ttacgaataa ctcatgactg accttttttg ataatacttg ttcaactagc
     1921 atttgaataa aaaacgactt accactacct gtttgcccaa ctaatcccat atgatgaaac
     1981 ggtacagttg cacgctcatc taaacgtagg gagtaatcgt cagttttctt gtttgaccac
     2041 tcaatcaagt cacttgctga tttaatctca agttgttttt gtgaatctac atcatagaac
     2101 tcatagatgt accaattacg gtctttagac aaatattgac gttcacttac gtatccttct
     2161 aacgctgcat caattctcat gtcttctaac ttcttgtcga atttaattga gttttgaata
     2221 aacacttttc ctgatttcct tgcgcgatta tcatcaaatt caattttgat tatcggtaat
     2281 ttaactaaac gattatcaaa ctcacgttca tcctcgaatc tagcatcttt aacatgtctt
     2341 ctaattcttt ttatcatcaa ataataatgc acagaaaaaa atagaccgaa ccataattta
     2401 ttgcgaattg accaactaac gaacaaacca acaaccacca agcctacagg aaacaagaag
     2461 atatttgact caatatgtgg gagctgtatg atcgttattt gttcaacaat caatgggtac
     2521 agtttagaaa agaaaaacca taaaataaga aaaaatccaa tgaataaaaa tgaaataaaa
     2581 ccacgttgca tccgatacgt tttcttcaca tattccacgt cctaatattg tggttccgcc
     2641 cctgataagg ggcggtcaca ggtataaaag tgtatttgac gatagaaagg gaactgacta
     2701 ccgttcgttc tttacttcgg ttaaacaatg tctacatcat ctgctttgac agaaagttca
     2761 ttcatgaacg ttccatacac agaagctgtc ggattcacaa acgtgattct actaaatttc
     2821 ttataactct ttggaggttg tgtcactttc acatcaattt gctcaccagc atttaaatct
     2881 ggctgaacat cattgccttc atattttgtg ttatccgtcg caataacgca cttgtactta
     2941 gtacctaact cttttgtgac ttggccgtcc tcatactcgt tccacggttc atgagccatc
     3001 actcgaacgt ccttatcctt gaaaaaaacc tcggcatcga acttattaaa ctgtcttaat
     3061 ttcttcatag tgttttgcac tcctttattt ttgatctacc tatattttag ctaatacttg
     3121 ccattttatc aagacatgtc atcatgacaa ataacaa
//
LOCUS       DAZKNO010000204         3127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000204 DAZKNO010000000
VERSION     DAZKNO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.209"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(310..1620)
                     /locus_tag="ACG8MY_002502"
     CDS             complement(310..1620)
                     /locus_tag="ACG8MY_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0431656.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1632..3050)
                     /locus_tag="ACG8MY_002503"
     CDS             complement(1632..3050)
                     /locus_tag="ACG8MY_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0431657.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          967 a          640 c          482 g         1038 t
ORIGIN      
        1 cgattgtcaa ctctttttta aaagtttttt tcgatttcgt ttttcgaaat ctttcagcaa
       61 ctcatttatc ataacttatc gtttgttatt tgtcaagaac ttttttgaag tttttttcga
      121 tagaatcaag cttcgcaagt ggttcttttt aacgacttag ttatcttatc acgtctaaaa
      181 acatgtgtca actctttttt ggaattttca taaaatgttt caatgaagcg cgacaacgtt
      241 gtttaatata tcattaaaaa aaataaaggt caagctaaaa tattatttaa gcttgacctt
      301 attataattt caacgattgt caaaagtatt gtttgcagct gtgttgccag tttctataga
      361 taaatcacag cttttgaaac ataagttttg tactgcttcg attcgcccga attcttttgc
      421 agttatttgg caatgagaaa agcagacatc ttctataggc ttttcaggaa atgctactaa
      481 atcaaaagct cgggaaggaa gtgagtaacc tggagaaagg catgcctgta cgtttttgat
      541 gtgtatgtct ttgactttcg tcatttgctt tgattcaggt atttcttcag ccactgcttg
      601 ccaactttct ggcatttgag caaggtcatt gctatttttt ctattgtatt cattatgcca
      661 gtccattatc caagaaaaag gaaattgtac atcaatcata ttcagatttt ctaccctgat
      721 attttcgatc actccccctc tttcttttga agatttcatt cgaataccac agtctgactg
      781 aaaaaaatcg atatcgtgaa tatatacgtc actaactcca gcactaactt cgctaccaat
      841 agttacaccg tatcctgaac ggattttaca tctagcaacc tcgattcttg atgattttcg
      901 attgactctt gcaccattgc catctctgcc agacttaata gcaatacaat catcaccaca
      961 ggccaaatca cattcataga cacgcacatt tgttgaagaa tcaatatcaa tcccatctgt
     1021 acttggtcca tcattgttgc ggatgattac tttttctact aatacatcgt tagaataagt
     1081 gatttgcaag ttccaaaaac ctgatttctg aaaaatcaaa tcacgaacaa caacatgttc
     1141 tgcatgatac agtaaacaag cgcgtggacg tttaatagca taatccgcaa tccaacgaag
     1201 tcccttttga tcgtattcag ctcttgtccc gcttttttga tcttttcccc aatacagctc
     1261 ccaccaatgg tctccttgac cgtcgattat tccttttcct tctataagaa tatctttcgc
     1321 cgagagaaca ttcaaaatcg ctgctggcca tttcatttct acgccggcta ctcgtgtatc
     1381 gatttctgga aatgcttcga tatctgttct ccccttgagg actgcacctt cttcaaagat
     1441 aagatgactc ttatctttta aaaaaagggc tccgactaaa taaatgcctt ttggaataaa
     1501 tacaggtaag tgttctttcg caccttgatc gattgctttt tgcagtatta ttgtatcatc
     1561 ttgttgagtg tcgtttgttt taaagttcaa agcattgata atcccttttt ttgtccgcaa
     1621 tacgtttctc cctatctttc attgattttt ttcaattctt caccaataaa tacagttttt
     1681 gaacgattca gtgggtaacc tactagcaaa atgattgcgg atacaaataa gcagaatgct
     1741 ggtaacaagt ttgccatcgt ataaatcctt tcttgtacta aaactgattg gatggctcca
     1801 cctgttgtag acgattggta accaatgagt gccagcataa atccgcctac cgctcctgca
     1861 catgcttgtc ctacttttct cgcaaaagaa tttactccat atactgttcc atcttctctt
     1921 aagcctgtct tatattgatg ataatcaatc acgtcagtaa tgaatgccca gaccatcaaa
     1981 ttgaataaac cagatcctaa agtagcgata aataaaagaa tcagatatac ccatgcattt
     2041 gttatatgaa ggaaatacat cagcaagtac atacatgaag aagcaagtaa cgcaaatacg
     2101 cttgcttcct ttttaccaaa taattttgtc agctttgtag caaatggtgc taaaactact
     2161 actgttccgt aagtaaataa aagagcaaca gacattgctt gtttattatg gaagtaatca
     2221 ttgaataaat aagtcatatt cgtcccagct aaaatctgat tgatcacaat gaaaatgtca
     2281 acaatgacta aaataatcag tgctcgatta gctaacaaac cttttaccaa ctgagaagca
     2341 gatacttttt cgtttttgac aactcttact ctttcagtag tcaatttaca agttaacata
     2401 tatgcgacaa atgctattgc agcacatccg attgaaatcc aaaagaaatg attccctgaa
     2461 acgacttgtt gtccactctt gttatgggta tacattaaaa tcggaatgaa gaatcctgta
     2521 gttgatccgc caatcgcaga accaatgctt cggaacgtgg acaacgaggt gcggtcatta
     2581 ggatttgagc taattgctgc tgccattgct ccatacggga tattgattgt tgagagaaaa
     2641 atcccataaa caatgtatga aacaaaaata taagcaatct tcaccgtcat tggaagatgt
     2701 tggacgaatg gcaaaaacaa aatgacagtc actaagcaaa atggatactt catttttctg
     2761 atccaaggaa taaatcggcc agtttctgtt aactctgaga catcacataa tcttccaacc
     2821 atcacatcag caaacgcatc caaaattctc gaaatcaaga ataacgtacc tacgattgcc
     2881 cctgaaacgc caagaacatt cgtataataa atcatcaaga aactattgac taaacctaaa
     2941 ataaaacagt tacctagatc accgaacatg tagcctatct tatcggaaat cccaaattcc
     3001 tttttttctt tttttattgt atttgctgta acagcatttt gtgaaagcat ttgaattcct
     3061 cctacctctt ctagtaaacg ttttcaaaat tataactctt tttttcttgt ttttatattc
     3121 acaatgt
//
LOCUS       DAZKNO010000205         3030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000205 DAZKNO010000000
VERSION     DAZKNO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.210"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..861)
                     /locus_tag="ACG8MY_002504"
     CDS             complement(247..861)
                     /locus_tag="ACG8MY_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0431658.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1467..1844)
                     /locus_tag="ACG8MY_002505"
     CDS             complement(1467..1844)
                     /locus_tag="ACG8MY_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431659.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2144..3025)
                     /gene="galU"
                     /locus_tag="ACG8MY_002506"
     CDS             complement(2144..3025)
                     /gene="galU"
                     /locus_tag="ACG8MY_002506"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0431660.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          918 a          635 c          468 g         1009 t
ORIGIN      
        1 actacttttt atttttctga cactacatat tcttataact catttctcat aactgtgaaa
       61 caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct
      121 taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt
      181 ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt
      241 ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg
      301 ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg
      361 caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc
      421 aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt
      481 aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg
      541 gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc
      601 aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat
      661 caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag
      721 tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt
      781 gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata
      841 gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa
      901 cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt
      961 ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc
     1021 aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag
     1081 attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg
     1141 gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct
     1201 ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag
     1261 caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg
     1321 aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa
     1381 aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat
     1441 ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg
     1501 agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt
     1561 cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg
     1621 caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct
     1681 gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat
     1741 agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg
     1801 agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt
     1861 tttttataat taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca
     1921 tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata
     1981 atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact
     2041 gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta
     2101 ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta
     2161 gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa
     2221 gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc
     2281 ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta
     2341 gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag
     2401 tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt
     2461 ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc
     2521 cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt
     2581 cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc
     2641 ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc
     2701 ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa
     2761 ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg
     2821 catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga
     2881 ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa
     2941 gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag
     3001 gaatgactgc ttttttgacc ttcacttgct
//
LOCUS       DAZKNO010000206         3016 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000206 DAZKNO010000000
VERSION     DAZKNO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.211"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..934
                     /locus_tag="ACG8MY_002507"
     CDS             104..934
                     /locus_tag="ACG8MY_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0431661.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(1178..1427)
                     /locus_tag="ACG8MY_002508"
                     /pseudo
     CDS             complement(1178..1427)
                     /locus_tag="ACG8MY_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1618..1812)
                     /locus_tag="ACG8MY_002509"
     CDS             complement(1618..1812)
                     /locus_tag="ACG8MY_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431662.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(2317..2502)
                     /locus_tag="ACG8MY_002510"
     CDS             complement(2317..2502)
                     /locus_tag="ACG8MY_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431663.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT          992 a          570 c          517 g          937 t
ORIGIN      
        1 aatgatacca atactttata taattcgcga gcgctttctt tgaatttttt aatctttcgt
       61 agcagaatga gaatgtatta aaaatagatt aggaggaatt ttgatgttta gacaccagaa
      121 agaattgcag tttgaagcaa aaccagatcg tccgaatcca ttaatcgcga aatatctgca
      181 ggagctgatc ggcggccaat atggcgagat gagtgtagct atgcagtacc tgttccaagg
      241 ctggagctgc cgaggagaag aaaaatataa agatatgctg atggacattg caacagaaga
      301 aatcggccac gtggaaatgt tggcgacaat ggttgctcgt ttattagagg gtgcgcctgt
      361 tgaagatcaa gaagcagcga tgcaaaaaga tccaacaatc ggtgccatta tctctggaat
      421 gaatccacag cacgcgatcg tagctggctt aggacctcgt cctgctgaca gcgtgggcaa
      481 tccatggagc ggtgcctata tcattgcgag tggcaacttg ttagcagact ttagagctaa
      541 tttaaatgcc gaatcacaag gaagacttca agtagcacgt atttacgaaa tgatcgatga
      601 tcgtggcgta aaagatatgc tgtctgttct ccttgcaaga gatagctatc atcaaaatct
      661 atgggaatgt gcagtgaaag aattagaaga aaaagaaggt tcaatcttag tcccaagtac
      721 tttcccgcgt gacaaagaac gaaaagacat tgcttatgac ttctataact tctcagaggg
      781 agaccaaagt agtgaaggtt cttgggctag tggcaaagca ctggatggcg aaggcgaata
      841 tcaatggttg aaagaaccta agatcgaagg cgctgcacca aaattagaac cagtaacacc
      901 aaaaatgtat gggacacctc cattcaagaa ataaatatct acccgtagaa acacccgttt
      961 tttgttccac acgctttgtg agcaaaactg actaaagtca ttaatcataa ccacccttca
     1021 aaagggtggt tttctctaga gctataagct caaatcaccg gccagcgcct aaagacgctg
     1081 gctttcatat tttatgttca agccatattg cctttgccag ttttgcccac ctcttaaaag
     1141 gtttatgtac tactccttag cccttaaaag gatcgtctta ctctcgtaca ctcagcttat
     1201 ctaacgcact atcatgtttt tcttgttctt gaatgtactt tttgatggtc gcttcatttt
     1261 gtcctacttt tgtcacatag tatccttcca cccaaaagtg tcgataccca aatttatatt
     1321 ttagatttgc acaatctctt gatgatctcc tgaatattgg ctcgatattg attataaatt
     1381 atttttcttc tatactttgg tgtgaataca atgtggtatt tacacatcta ctttgtacgg
     1441 gctaaactat ttccctttat tgctccaccc gcatagcaag tggttttgta tttcaatcgc
     1501 tcggcgcgct caattaggtc taaagacaat aaatctaaaa acccgctgct tcactgacag
     1561 aaacaacggg ttttcagaaa aaaatgaaat gaaaaagatc tcttacggca atctatttta
     1621 tttttttgat tttttataaa tggaaaatgc ttcaagaagt ccaagaaata aagccgtata
     1681 aaaacaagaa cctatgaaat ctttatttat tagatattca acagaaatcc cgataacact
     1741 agccaacaat atatttatca gaagtgtaaa ataaggatgc tttttataga atagaactag
     1801 tgttgttttc atagactctc ctttcttcat atttccaatt aatcacaaat tcacggcatt
     1861 actagaaaaa ttaaaggggc gcagaagtgg aaaaatactc ttcgactttc cctacaaacc
     1921 aggccatctt gtgtttaata actataccaa atctaaggta cctctaaatc taaattccat
     1981 ttgtggcggt gacctccaat ttttaactct tctatctccc atccagtata cataaccttg
     2041 atacttcgac cacttatata tcgaacacac aaatacctat ccattgcagg agtactacct
     2101 aacttagttg ctacttgcat ctaaataaca gctgaaaaga taggacttac tcttagtaaa
     2161 gtatctgaac tattaatttt taaactatca tttttatcct caactacatg cacatctgtt
     2221 gcaacttcac tttctgtctc atccagataa atgattaagg aatcgggaat tgcaatatct
     2281 tcagctgacg ctggaatagt tgagctaaat tagtcatcat atctttatta taaaataacc
     2341 aacagcaaaa aatacaatga aaaacaaact tggaactaat gtatcattaa taaatgttga
     2401 aaaattcagc aactcattac tgtatgattt tagcagccca accaacatat accctataaa
     2461 agctaataag atggttagga atcctttaga atttttactc attcatagac acttccttaa
     2521 ataactttta ttatagaagg tattgtaaat aataatattg acaaaaagtg actcaaaata
     2581 aaaaaaaact tacattatac tggaatgaac tgtattgata gtgtacctaa aagctctact
     2641 gacataagat cgtgtaatcg gtaaagtaat tttattaatt atacgggatg taatcaatta
     2701 gagaataaac taatcataaa aggtactttt acattcataa ttgtcgcttt gagattctac
     2761 tttggttcac tatctgaccc aattgcacat tttcgatcat tacaagtatt ggtgtcgtta
     2821 gaacaactaa ataaaaacta gcggtagatt tttcagaatc tatgctaatt tctgtatgga
     2881 tgttccagtg atggcaaatt tctcttttca ttgtttttct tgtcgcatga tatgcctttt
     2941 tgtgttattc agttaaacta tagaaattct ttcctagaca gatcttgatc ccaaatttta
     3001 cgtttttgag catcga
//
LOCUS       DAZKNO010000207         2991 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000207 DAZKNO010000000
VERSION     DAZKNO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.212"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..1590
                     /locus_tag="ACG8MY_002511"
     CDS             133..1590
                     /locus_tag="ACG8MY_002511"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HFQ0431664.1"
                     /translation="MKKNQPNVVLIVVDQMRADALSLNSQDKIISTPTLDMMASQGYN
                     FENCYSPVPSCVPARAALLTGLDQETSGRVGYEDEVPWNFKNTLPEVFKEQGYQTECI
                     GKMHVYPSRKRLGFDHVLLHDGYLHVDRKYDKSYGEQFEYSSDYLMFLKESLGSDADL
                     IDDGLNCNSWEARPWMYPEKFHPTNWVVSEGINFLRRKDPTVPFFLKLSFEKPHAPLN
                     PPKYYFDMYMDRLPDTLDLHIGNWEKLEHVVPDVCALRGKLKEDDQRRMLAGYYGLIS
                     HIDHQINRFLMALKEFRHDKDTIIWFISDHGDQLGEHYLFRKGYPYQGSIRIPSFIYD
                     PGDLISAKKHGIKELVKIQDIFPSLVDLVLGQYVNTDGKSVKQLLFGNCEGWRREIHG
                     EHSLGLDSSQYILTEKWKFIWFPVKNTYQLFDMINDPNEMKNLYYDKKYESIIYEMKH
                     KLVGYLKGREEGFVKNGQLIQIGISNIVSTLKSKK"
     gene            1781..1947
                     /locus_tag="ACG8MY_002512"
                     /pseudo
     CDS             1781..1947
                     /locus_tag="ACG8MY_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1056 a          391 c          532 g         1012 t
ORIGIN      
        1 catacgctat aaagtttggt aatccattta tatttcttgt tatcacaaaa aatatattct
       61 ttcaaaagga aatatcaatt tcttaaatca agagagatta tttggatatt ttgaatgatt
      121 ggagttttta atatgaaaaa gaatcaaccc aatgttgttt taattgtagt agatcagatg
      181 agagcagatg cgttatcttt gaatagccaa gataaaatca ttagtacgcc tacattagat
      241 atgatggcta gtcaaggata taattttgag aattgttatt cgcctgttcc aagttgtgtt
      301 ccagcaagag cagcgttatt aacaggatta gatcaagaaa cttcgggaag agtgggttat
      361 gaagatgagg ttccatggaa ttttaaaaat actcttccag aagtttttaa agaacaggga
      421 tatcaaactg agtgtattgg gaaaatgcat gtgtatcctt caagaaaacg gttgggattt
      481 gatcatgtac tattgcatga cgggtatctt catgttgatc gaaaatatga caaatcttat
      541 ggagagcagt ttgaatattc tagtgattac ttaatgtttc taaaagaatc attgggctca
      601 gatgcagatt tgattgatga tgggttaaat tgtaattcat gggaagcacg accatggatg
      661 tatcctgaaa aatttcatcc aacaaattgg gtggtttcag aaggaataaa ctttttaagg
      721 cggaaagatc ctacagtacc atttttctta aaattatcat ttgagaaacc tcatgctcct
      781 ttgaatccac caaaatatta ttttgatatg tatatggata gattaccaga cactctagat
      841 ttgcatattg gtaattggga gaaattagaa catgttgttc cagatgtttg tgccttaaga
      901 ggaaaattaa aagaggatga tcaaagaaga atgcttgctg gatactatgg ccttatttct
      961 catatagatc atcagatcaa tcgcttttta atggctttaa aagaatttag acatgataaa
     1021 gatacaatca tttggttcat ttcagatcat ggagatcaat taggagaaca ttacttattc
     1081 agaaaagggt atccttacca agggagtatt cgtatcccgt cgtttattta cgatcctggt
     1141 gatctgatct cagcaaaaaa acatggaatt aaagagcttg taaaaataca ggatatattt
     1201 ccttcattag ttgatttagt attaggacaa tatgtaaata cagatggaaa aagtgttaaa
     1261 cagttgctct ttggaaactg tgaaggatgg cggcgagaaa ttcatggaga acatagttta
     1321 gggctggatt ctagtcaata tatattaacg gagaaatgga aatttatatg gtttcccgta
     1381 aaaaatacgt atcaattatt tgatatgata aatgatccaa atgagatgaa aaacctatat
     1441 tatgataaaa aatacgaatc tattatttat gaaatgaaac ataaattagt aggatattta
     1501 aaaggtagag aagaggggtt tgtcaaaaac ggacagttaa ttcaaatagg aatttcaaat
     1561 atcgtttcaa ctttaaaaag taaaaaataa actaaaagtg aagatgcaat aaaaaatcaa
     1621 catagggtat actgagtcaa aatatactca gatacaggca aaactttgcg tctttattta
     1681 tttcaaaaaa atcaaatatt cggatggtaa caaacatact actttgtttg ggataaaatc
     1741 agatctgagt cagagtaagt tcattctgat tcagtttttt atgtctaacg acgataaaag
     1801 tttagcacat acaagatgga attgtaagta tcccatagtc tttaccccgg agtatcgatg
     1861 aaaagtgatt tatggaaaat taagagtaag taactgttgg tgatagtatc accccaaatt
     1921 agtatttcca gttttatgag ctatttatta aataaattct atacgggatt tgaagatcct
     1981 gaaggtgaaa tcgtcattta tcagaataaa taaatcaaat agtaaaagtt caaagaatga
     2041 ggggaacatt agtttttatt gcttagttta atcccagtaa cgatttctgc aagatgtaaa
     2101 aggtctttca tttcataagt tttcagaagc aagttcatac gcactaactc actttcgatt
     2161 gagtagcaat cgtcaacaaa ctttactatc agctgttatt tttattcttt cgcgttatct
     2221 aaataccatc aatttataag ttgtaggtgt atttaataaa agaacaaata actttagtat
     2281 ttttattaat ctaacttaca aactagtagt atagggtata gtaaaaatat gataaaataa
     2341 atagtcgtta tcttaatcca actatataga aaatttacgt tttctatata gttggatgtt
     2401 tacaagttat atctggttta ataagttttt ttctgttagt catggcatgt tttattgtgc
     2461 tggttcatca atatattgaa atgtactaaa atgaaatttt cagttatggt ataatattta
     2521 tgctgtctgc taccccggcc tattatggca aggggaggag gttgtattat gaattacctt
     2581 ttcgatatgt tttttgcaat cttagcagaa gtatccgcgc attacatctg taaatggctt
     2641 gattgcaaaa ttattgccaa aaaagacagt aagcctaccc gttaatctga atagaacgta
     2701 aaagaaaacc actagcccgc cagctagtgg tttttggtgt atcatgaatt accttttcgt
     2761 taaaacaatt ataccatatt tcctaaaaaa tacaattaat agacgaatta tcattaccac
     2821 tttgatgaaa aaagctgaca taaatttttc ttttattttt ataatgaata aacatttatc
     2881 aataagcttt tgaaaggcag gatcgtactt tgttgatgat tgagatattg ctacatcaat
     2941 ctcagcagtt aagatttttt tgaattgttg cttgaatttt ttctttatct a
//
LOCUS       DAZKNO010000208         2990 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000208 DAZKNO010000000
VERSION     DAZKNO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.213"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..1119
                     /locus_tag="ACG8MY_002513"
     CDS             106..1119
                     /locus_tag="ACG8MY_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0431665.1"
                     /translation="MTKKNRVTIRDVAQAAGVSISTVSKALNSLDGVSPETRVEIQNI
                     ASRLNYVPNLMGKQLKSGKTKMIGVYTNRISGPYFNSLIDEIAFESKLHGYGLNVIVS
                     DERDVIMSSLLGNFVDGAIVVEDVLNQKDIETINSNGVATVFINRIVADKKVGSVAFD
                     SFEKGYLAGNYLLNLGHKKIGYVNGVDDIYDNDQRFQGFKAALSKVGLTYNEDFTIAG
                     NFVEEDAYANTLAFIDNQPAELPSAFLAANDMSAVGLIKALTEKNYSVPEDFSVVGFD
                     DVEVLKYFKPSLTTIHNSTKEQGKKAIEHLLEMMGGKSVGRVIEVKGTLMIRETAIEY
                     TGE"
     gene            1126..1476
                     /locus_tag="ACG8MY_002514"
     CDS             1126..1476
                     /locus_tag="ACG8MY_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0431666.1"
                     /translation="MYVGIDVGGTSIKYGLVDSKGNIVAKDTLVASFEKEELLQNLAD
                     IVEKYVSSSPETIEGVGISMPGIVQEDGFLLTAGAIRSCYGINLKEEMEKRTGILTKE
                     VIDSNGANLDWENK"
     gene            1630..2799
                     /locus_tag="ACG8MY_002515"
     CDS             1630..2799
                     /locus_tag="ACG8MY_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HFQ0431667.1"
                     /translation="MATERSFATEENKTNHVSAETALVATSKTTVSKDNPTVFDFPFN
                     GEDNRMHRADGWSNGNMFNTTWRASQVNFEDGKMQLSINKDEENTKIPYISGEYRSNQ
                     NFHYGLYEVNMKPIKNDGVVSSFFTYTGPSENNPWDEIDIEFLGKDTTGVQFNYFTNG
                     LGNKEYYHNLGFDASEAFHTYAFEWLPDSITWFVDGEKIYTATENIPQTPGKIMMNVW
                     PGTGVDSWLNPFDGTVPLKAEYDWIKFTQYKIVEEDVTPKPEEPTTEPTTEPTTEPTT
                     EPTTEPTTEPTTEPTESSTTGSSTTESSTTNSSGNTTPPATTETTNTTSSSTSQSSKA
                     AGKTTTPKNNSTTSKKDLPATGENTHASYWTAFGGMLILAGLGGALFFKNKNRKA"
BASE COUNT         1038 a          494 c          675 g          783 t
ORIGIN      
        1 cttttatttt ttataaaaaa agcttgtatc caaaaaaata cagtgttata cttcaatcga
       61 agtaaagaaa aacgttttct ttaaaaagta catgaagtga ataaaatgac aaaaaaaaat
      121 cgggtaacga ttagggatgt ggcacaagca gctggcgttt caatctcgac ggtttccaaa
      181 gcgttaaaca gtttggatgg cgtttcacct gaaacccggg tcgaaattca aaatattgcc
      241 agccgtttaa actatgtgcc caacttaatg gggaaacagt taaagtctgg taaaacgaag
      301 atgatcgggg tttatacgaa tcggatcagc ggtccttatt ttaactcact aattgatgag
      361 attgcttttg agtctaagtt acatggatat ggtttaaatg taattgtctc tgatgagcgg
      421 gatgtaatta tgagtagtct gttaggtaac tttgttgatg gggcgattgt ggtggaggat
      481 gtcttaaatc agaaagacat tgaaaccatt aattccaacg gcgtggcgac ggtttttatt
      541 aaccgcatcg tagcggataa aaaagtcggc agtgtggcct ttgattcgtt tgaaaaaggt
      601 tatctggcgg ggaattattt attaaactta ggtcacaaaa aaattggtta tgtcaacggg
      661 gtcgatgata tttacgataa cgaccaacgt tttcaaggat ttaaagcagc cttatcaaaa
      721 gtcggtttga cttataatga ggactttacg attgccggta actttgtaga agaagatgcc
      781 tatgccaata cgttagcctt tattgacaat caaccagcag agttaccgtc ggccttttta
      841 gcggcgaatg atatgagtgc ggtggggttg attaaagcct taactgaaaa aaattattct
      901 gtaccggaag attttagtgt tgttggtttt gatgatgtgg aagttttgaa atatttcaaa
      961 ccaagcttaa caacgattca taattcaacg aaggaacaag gtaaaaaagc gattgaacat
     1021 ttattagaga tgatgggtgg gaaatcagtc ggtcgtgtga ttgaagttaa gggcacgctg
     1081 atgattcgag aaacagcaat agaatacaca ggagagtgat tgagtatgta cgttgggatt
     1141 gacgttgggg gcacaagtat taaatacggt ttagttgatt caaaaggaaa catcgtggcc
     1201 aaagatacgt tagtcgcgag tttcgaaaaa gaggagctct tacaaaactt agcggacatc
     1261 gtggagaaat atgtaagcag ttcgcctgaa acaatcgaag gtgtgggaat cagcatgcca
     1321 gggattgtcc aagaagatgg gtttttatta accgctgggg cgattcgttc ttgttacggt
     1381 atcaatctaa aagaagagat ggaaaaacgt acggggattt taacaaaaga agtgattgat
     1441 tcaaacgggg ctaacttaga ctgggaaaat aaataagtca caaagaaacg tcaaacatta
     1501 acagaaaaat aaatgtttgg catttccttt acgaaaaaat gaaaacgatt gcttaaaaac
     1561 aataactgga gggaatttat gaataaaaag agtgcaattt tactattaag ctgtggggta
     1621 tttttaagtt tggctactga gcggagcttc gcaacggagg agaacaaaac aaatcatgtg
     1681 agtgctgaaa cggcactagt cgccaccagt aaaacgacgg tttctaaaga caatccaacg
     1741 gtctttgact ttcctttcaa tggagaagat aaccggatgc atcgggccga tggttggagc
     1801 aatgggaata tgtttaatac aacgtggcgg gcctctcaag taaacttcga agatggtaaa
     1861 atgcaacttt cgattaataa agacgaggaa aatacaaaaa ttccgtatat tagtggggaa
     1921 taccgttcca atcaaaactt ccattacggt ttatacgaag ttaatatgaa accaattaaa
     1981 aatgatgggg tagtgtcatc tttctttacg tataccggcc caagtgaaaa taatccctgg
     2041 gatgaaattg atattgagtt tttaggtaaa gatacgactg gcgtacaatt taattatttt
     2101 acgaatggct tgggcaataa agaatactat cataatttag gtttcgacgc ctcagaagca
     2161 ttccatacgt atgcctttga atggttacca gattcgatca catggtttgt cgatggtgaa
     2221 aaaatctata ccgcgactga aaatattccg caaacaccag gcaaaattat gatgaatgtt
     2281 tggccgggta ctggagttga ctcatggtta aatccctttg atggaacggt accattaaaa
     2341 gccgaatacg actggattaa atttacgcaa tataaaatcg ttgaggaaga tgtcacgcct
     2401 aaaccagaag aaccaacgac agaaccaacg acagaaccaa caacagaacc aacgacagaa
     2461 ccaacaacag aaccaacaac agaaccaaca acagaaccaa cggaatcttc aacaacggga
     2521 agttcgacaa cggaatcttc aacaactaac agtagcggaa atacgacacc accagcaaca
     2581 actgaaacaa ctaacacaac ctcaagctca actagtcagt cgtccaaagc cgcaggcaaa
     2641 acaacgacgc caaaaaataa tagtacaact agtaaaaaag atttacctgc tacaggagag
     2701 aatacacatg ctagctattg gactgctttt ggcggaatgc ttattttagc tggtctaggc
     2761 ggcgccttat tctttaaaaa taaaaaccgt aaagcgtaat cggaggggat ttatgaaagc
     2821 gaaaggtgtt atgctagtat taggctgtag cttattgctt ggtttagtaa ttaatcgcag
     2881 tttggcccat gagaaaaaaa gtaaaaatag tggcgaggag acggagagta cgatggtaga
     2941 aaataaaaat aacgctaaag ctcagacaac tgtctttgac tttccactag
//
LOCUS       DAZKNO010000209         2947 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000209 DAZKNO010000000
VERSION     DAZKNO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.214"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(449..865)
                     /locus_tag="ACG8MY_002516"
     CDS             complement(449..865)
                     /locus_tag="ACG8MY_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431668.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="ACG8MY_002517"
     CDS             complement(867..1148)
                     /locus_tag="ACG8MY_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431669.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="ACG8MY_002518"
     CDS             1948..2922
                     /locus_tag="ACG8MY_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0431670.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DAZKNO010000210         2941 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000210 DAZKNO010000000
VERSION     DAZKNO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.215"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..567)
                     /locus_tag="ACG8MY_002519"
     CDS             complement(205..567)
                     /locus_tag="ACG8MY_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431671.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(641..1021)
                     /locus_tag="ACG8MY_002520"
     CDS             complement(641..1021)
                     /locus_tag="ACG8MY_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0431672.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1103..2278
                     /locus_tag="ACG8MY_002521"
     CDS             1103..2278
                     /locus_tag="ACG8MY_002521"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0431673.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2355..2939)
                     /locus_tag="ACG8MY_002522"
     CDS             complement(2355..2939)
                     /locus_tag="ACG8MY_002522"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0431674.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          918 a          566 c          489 g          968 t
ORIGIN      
        1 caactataaa ttaagcatta ctttgtgaaa tgatttttcg atcaattatt attattcgtt
       61 atacaaccgt tttcaacata ttttgaaaat aaaatgcact aaaaaactat ctttagcaga
      121 acatttcatt gtattttttg gaccaaacaa taaaaaaaga agtttgaaaa aaattcaaac
      181 ttctttgttc gcttaatctg tattttagaa gaaaggcact ccctctactt cgtaagttcc
      241 aagaacagag cgcatttgac ctggttcttc ccaaatatat ccttcgccta attcttctct
      301 ttgattcttt tgttcagttt caaagacaaa caacggaaca ttttcctctt taatatgtgt
      361 atttgtcaat tccatcaaac taatcttgct aacatcgata tgaactattt cttttaagaa
      421 attaagtata ttcgctaaac cagtctttcc ttcagaaagt tccgctaacg caaattccaa
      481 cttgtcatca attgaatgga ccaaaaattt tttagtacca tcctccaaat gcaacataat
      541 tgtaccagca actcttttgc ttgccaaaat ttccacctcg atcaacttgt tctatggctt
      601 acataaaaat tctagcacat tttcagaaaa aatacacttt ttatttctta ttttctttcc
      661 gttctagttc agataatgct tcttctatcc aaataggtaa ctgctccgca atagtggtaa
      721 atccgatctt acgattttta ttcgtgaaat acctttttct gcgatatgga acagctggat
      781 ttgttttttc taacgttctt aaagataaac tgattttttt ggaatattca tcaatgtcga
      841 tgatctgaac tgttactggc tgtccgactt ttaacaatga atggatattt ttcgtataac
      901 cggattgtac ttctgaaaca tgaataagtc cttgcgtctc gttatcaaga gaaacgaatg
      961 cgccataagg ctgtatccct gtcacttttc cttcaattat ttgtccaatt ttataagtca
     1021 tctacttccc tctcttcgca tttatcatta tactgtaaat agctcaaaaa aagaagcgac
     1081 gaaaatgact ggaggtctta agatgatcga atttgataaa cagatttcaa gaaaagatac
     1141 agaaagtgta aaatgggacg caattgctga gacatatcaa atggatgact tacttccttt
     1201 gtgggtagca gatatggact tcttgtctcc cggtggggta gctaaagcgt ttagtgaata
     1261 catcaaacat ggaatctttg gttatgccac tctgtctgat aaactatatc aggctatcat
     1321 tcattgggaa cgccaacacc atgctgtgga gttgacgaaa gaaaatatcg tttttacaag
     1381 tggtgtgtta accagtttag ctgcagcagt acaaactttt acaaaaccag gcgattctat
     1441 tttgattcat gacccagttt atcctccatt ttcctctatt atagaaacaa atcaacgtca
     1501 catcgttcga agctctttgc tagaaaaaaa taatcatttc atcatggact tgtctgacat
     1561 ggagaaaaaa atcgaagaaa acaatgtaaa agcaatgatt ttatgcaatc ctcacaatcc
     1621 tggagggcgt gtttggagca aagaagaatt gaaacagtta agcgaattgt gtttaaaaca
     1681 tcaagtgatt ctgtttagtg acgagatcca tcaagattta actttattcg atcatacatt
     1741 tacttccatg ctaacgattg atcctaattt agatgatcta ctagttgctt ttacttccgc
     1801 aaccaagact ttcaatctag ctgcaatcaa aaattcaatg gtatttatca aaaatccaga
     1861 gatgaaagct gattttgaaa aacatttagt gatgaaccaa cagcacgaaa tcaacacttt
     1921 tggactgatt ggaacccaag cggcttatga gacgggtgga gaatggttaa gccaactgat
     1981 tccttatcta gaaaaaaatg ttcaaacagt taatcagttt tttgaacagt atcttccgaa
     2041 agtgagaata atgaaccctg aaggaactta cttaatgtgg ttagatttta ccgcctatat
     2101 ttcagacgac caagaattgg agaacactct tgttcggaaa ggaaaagtcg tattaaatcc
     2161 tggcatcacg tttggtcctt ccggtcatgg gcatatgcgc ttgaatattg cttgtccaga
     2221 agaaacttta ctggaaggac ttaatcgaat caagtatgct ttagagacaa tcgattaaat
     2281 catttattca aaataaaagg acgagacctt actaggtttc gtcctttttc ataattactc
     2341 cgtaccatac caaactattc tgaaatttca attgtttcaa tcactacatc atgtacaggt
     2401 cggtcttgtg gaccacgttg tacatttgcg atttcatcta ctgtgtccat cccttctagt
     2461 acatgtccaa agactgtatg tcggaaatct agccaaggag ttccgccacc tttatacgct
     2521 tcaatgattt ctgatgggaa tccagcacct tctagttgtc caagcatatt agctggcaca
     2581 tttgtgttat ttacaataaa gaattggctt ccgtttgtgt ttggacctga atttgccata
     2641 gataaagctc cgcgaaggtt gaacagttca cgactgaatt catcttcaaa actttcgcca
     2701 taaatacttt cgccacccat tcctgttcca gttggatcgc cgccttggat catgaaatca
     2761 ggaataacgc ggtgaaaaat cacgccatca taataacctt ttttggaaag ttcaatgaaa
     2821 ttttgaaccg tcttcggtgc tagttcaggg aataattgaa cagtaatgtc accacggttt
     2881 gttttgataa cagcttttgg tcctttttca ctttttaaat ctagttgtgg gaatgccata
     2941 t
//
LOCUS       DAZKNO010000211         2866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000211 DAZKNO010000000
VERSION     DAZKNO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.216"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..287)
                     /locus_tag="ACG8MY_002523"
     CDS             complement(<1..287)
                     /locus_tag="ACG8MY_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HFQ0431675.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWK"
     gene            437..919
                     /locus_tag="ACG8MY_002524"
     CDS             437..919
                     /locus_tag="ACG8MY_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0431676.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            956..1966
                     /locus_tag="ACG8MY_002525"
     CDS             956..1966
                     /locus_tag="ACG8MY_002525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0431677.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2081..2794)
                     /locus_tag="ACG8MY_002526"
     CDS             complement(2081..2794)
                     /locus_tag="ACG8MY_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431678.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT          959 a          467 c          574 g          866 t
ORIGIN      
        1 cttttccaag gaactttgat gacgttatcc gtatatacgt agcctgcttc ttggatattt
       61 ttttcaaggg cacgaatcgc tgctttttcc ggtgcgatag aaaaatgatt tttagcaaaa
      121 ctaaatgcaa taataaaggt accatggtca gtaaacatag gttggttcca ttttacagtt
      181 gtgtctagct ggggatacgt ttctgttatc ttagcaaaaa ggtctttgat cttttgttga
      241 tgttcttttt ctttgatctg attgatgatc ggctgaaatt tttccattct aaaacgcctc
      301 cattaagtgg tttgatttat tttaccagaa aaacaatagt tatgtataaa aaacgaacaa
      361 aacacaagta atgttgtaac cgctatattt atatgataag atgtctcaaa taataaaata
      421 gaaaagagac ataaaaatga ttgacttggc aaagaaattc ttacacgaaa atatatattt
      481 tgagttgtat catcatcgag ccatttatac aaatgaagat gctttggtaa taaagaaaga
      541 acaaggattt acaggtacag agaccaaaag cttattctta aaagataaaa aagataataa
      601 ctacgtatat ctgactttta ctacgaaaca aacggatttc aaagcattag ggaagatcgt
      661 cggtaaaagg ctttctattg tttcatcaga acgcatggaa gaacaaacgg gacaaaagca
      721 gggcgctgtt tctccgtttg gttatgaaaa acagattccg attattattg atgaagagct
      781 cttatcaaat gaaaaacttg tttttgcacc aggtagacca gatcaaacga tggtcatcca
      841 aacggttgac ttaaacaaaa tcatccagct acttgagatg ccgatatttt tgatgccggc
      901 aaacgaaaaa aacaattaac caagtaaaat agtgcatact tggaggtgaa gcaacatgat
      961 aagttatcca ttattagaaa gaaatgcaac gattggggtt actgcaccat cctcaggcgt
     1021 atcagaggaa caccattcgt tactcgaatc agctttaaag agaatggaag aaaaatcttt
     1081 ccaagtagtt actggaaaga cgctttggac gcaagataag gaaaaatcag gtccagcaag
     1141 aaaacgagca gatgaattca atcaaatgat gcaagatgaa aatatccaaa tgatctttcc
     1201 gccttgggga ggagaattat tgattgagat tctcgatttt gtcgattttg atgctctaca
     1261 accaaaatgg atagtaggtt attcagatat tagtacgttg ctacttgctg tcacattaaa
     1321 aacagggata gccactgcac atggaaccaa ccttattgat ttaagaggag agtacttgga
     1381 tgaaacgaca gcaatgtggg aaagtgtact gaaaacaaga gaaggagaat cgattctaca
     1441 gcaaccttct gaaaagtatc aaaaagagtg gccccatgag cattcatcat cttctgtttt
     1501 ccatctcact gaaccaacaa attggcaatc agtcagcgga gatgcagtaa ggatgaaggg
     1561 acgattatta ggtggctgta ttgatacgat cagtcattta atcggtaccc cttatggaga
     1621 tgtccaatct tttaaaagaa agtataatga tggaaagccg atcttgtggt ttctagaaaa
     1681 ttgtgagcta tcgtcagttg atttaagaag atcacttgtt cagatgaaac tggcaggatg
     1741 gtttgatggc agctcgggga tcatgttcgg tagaagtgca gcagaggatt tgttagatta
     1801 taaaatgaag gatgtatatg aagatctgtc aaaggaatta aaactaccga tcttgtatga
     1861 tattgattgt ggtcatgtac cgccacaact gacattgatc aatggtgcct atgcagaagt
     1921 agcggtagaa gatagaaaag cgactgtttt gcaaacgttt atttaaaaaa ttttttcctg
     1981 ccttggagtt tatcaaagac caacaaatct aaatagaaaa gaaaatgaaa gaagctgaca
     2041 gtattttgcc agcttctttt tgtatgatct attagaaatt ttaattatcc ataactatca
     2101 cattgatcag ctgtttcaca tcatttggat tacctgtagg aatagctact acagatgcat
     2161 tatcaataat ttcttgagtt gtttctctaa tgaacgctgg ataagatgtt tgatctgcag
     2221 gcatttgcca atgtgaagga actggataac tagtgattgt cccgtcatgg ttacttgaat
     2281 acactaaaac tccctggtaa ctgtatttcc cagtgagcat aaccacatct ttcggataga
     2341 ctgcactccc tgcatcataa ggggcgatag gtgttcctgc aggataatga ataacatgta
     2401 gttcgaaatc acccgatgta tctctaaaag tattacctct aattgctagc caaacacgtg
     2461 cgtactcaat ttgtagatca gaatatcctg ctaacggatc tattgactga gaagactgtg
     2521 aggaagttgt gtctattgtt gaagaagaaa tatttgtctc aactgtacta ctagatgtag
     2581 aagaagtatt gggctctgag actttactgc ttgttgaaga tgtattcgtt tcagtagaag
     2641 ctgatttctg agtacttgat ttttcagctg aactggtttg tttgattgaa cttttgtctg
     2701 tggtcgcaga tggatcacta ttttctttac taggagacgt acatcctaca aggaataaag
     2761 agaccaacaa taaaataaaa cctaactttt tcatataaaa tacctcatta tttttttaat
     2821 accagtatag cataatcctg aatttttgaa tccgtaaaac cttgtc
//
LOCUS       DAZKNO010000212         2852 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000212 DAZKNO010000000
VERSION     DAZKNO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.217"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..126)
                     /locus_tag="ACG8MY_002527"
     tRNA            complement(43..126)
                     /locus_tag="ACG8MY_002527"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(90..92),aa:Leu,seq:cag)
     gene            654..1154
                     /locus_tag="ACG8MY_002528"
     CDS             654..1154
                     /locus_tag="ACG8MY_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0431679.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1344..1688)
                     /locus_tag="ACG8MY_002529"
     CDS             complement(1344..1688)
                     /locus_tag="ACG8MY_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0431680.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1842..2762)
                     /locus_tag="ACG8MY_002530"
     CDS             complement(1842..2762)
                     /locus_tag="ACG8MY_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0431681.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT          897 a          541 c          462 g          952 t
ORIGIN      
        1 catcgctcca ggtgtacctt ctctttataa aaaaacaaat tatgcggcta agaagactcg
       61 aacttccacg aggtcaccct cactagcgcc tgaagctagc gcgtctgcca attccgccat
      121 agccgcatca catcttttag tttaggatta catgtgtttt ttgtcaagaa catatttttg
      181 ttctttttca tttctgtacg ccctccctta tgccatctac acaaataatt catccgagca
      241 actccatcat ctattgaggg ttggtgaaaa aatctgtttt atccatatga tcgatttcaa
      301 tgttaaacga acgactctgg gattagtaac tattttattt ttttcttcat ttcttttttc
      361 ctctttttct cgattcagct atcttctttc atttggatat tgattatttc ttctgttgat
      421 tactatcctt ctggaattct agacgctgaa gtaattcacg ttttctttct tctagagatc
      481 ataagtaacg aatgaacgaa cttcaaataa tttttttatt gtaattcata tttgtattgt
      541 tcgatgattc ttaatacgat atgacacaat aaataaaaat aggcatggct atttacaaca
      601 atagtgctat agtagaaaac aaatgaataa ggttacttaa agaggtgaat acgatgaaac
      661 tgatcaaact atgggaagca gatttaaaga aagcttatca gctacaaact agctttggac
      721 caaatgagag tggatttatc aacaatgctt atggctatac atttgctgaa tttttagatt
      781 atgtaaaaaa atgcgaaaaa acgtctttag gaatcgatct tcctgagagt tacgttcccg
      841 caaccgtctt tattttagaa agtgatgaac atgagtatgt aggaattttt aatttgcgcc
      901 ataaactgaa cgaaacatta agaaacggcg ctggccatat tggttacggc atctccccta
      961 attatcgtaa aaaaggatat gccacacaag gattagcatt agtactacaa gaggcaaaaa
     1021 aaatcggcat tgatgaggca cttttgtctg taaataaaaa aaatcttggc agtcttaaag
     1081 cacaacaaaa aaatggggct gcgatttatc gtgaagatga gaaagaatac tatacgaaaa
     1141 tcaaactgat gtaaaaaaga aggattccaa aaggaaaggg atttgaatgt cctttatcaa
     1201 tagaacacat ttatttaatt aacttataat atagcagaca aaattttata aacaaagaaa
     1261 ccttttatct cttttcttct agaaaggata aaaggctttt ttgtttttcc tatttgcttc
     1321 tcatagctag tcagccaaga tttctatcgt atccccaacg caatccgtgc ataacggctc
     1381 atcttatctg tcgtccatgc tggataccag actagtttga cttctgcttt cgttacttct
     1441 ggcacttctt ttaatgcgtc atggatagat tctgttaaaa tatctgctaa tggacagccc
     1501 atagttgtta gagtcatctt aatgactgtt tctccatttt caggattgaa ttctatatca
     1561 taaatcaagc ctaaattgac gatgtcgatt cctaattcag gatcaatcac catttccaag
     1621 gcagcaagaa tacgttcttt gattagttct atttcttgtc cagaccgttc ttggtttgct
     1681 tcgctcatcg tacgacactc cttaacacga tttttattta tcatactctt tttttagctg
     1741 cttgtaaatg agaatttaga agtaagattc tcgtttaaat ttataaaaat gttcttatta
     1801 gatgtaatag attaaattta ttctaaaata aacctgattc ctcatttttt ttgtgaacga
     1861 ttttttattt ctggcaatac gccagttgtt cgatacattt tttgttcgtt ttcatttgca
     1921 aaccggccaa atactgaatg agttggtaaa atattgataa atgcatcgtc gtcgatttca
     1981 tgaacgatct gatcaagatc atacatttcg tagcgggtga tgaccatcat gatcatgttc
     2041 ccttgttttt ttgaatagcc accgacagac ggcagtaatg tcattccacg taccatccgt
     2101 tttgctagtt cttctgttac ctcttctggc ttgacggtaa cgatcattgc tgttactttt
     2161 tgatgactcg tgtggatggc atcaattact tgagtcattg cataaatgga aataatcgta
     2221 tacaaggcac tttcccagtt aaagaagaag cctgcgatga ctacaatgat accatttaga
     2281 atcagcatgt agtttccgac tgtttttcca gttgtttgtg aaagtacaag agagatgata
     2341 tccatcccac cagttgtaaa gcccattttt aaagataatc ctacgccaag acctaataaa
     2401 acaccgccta ctagggcatt catcaaaata ttatcagtga tctgtccgac tggaagcagc
     2461 atcgtcatca ccgatacaca tacaacattc acaaaactaa ggatcgttgc tcgttttcct
     2521 actttgagaa accctaatac aaacacaggg atattcaaaa gaaaaataaa tgttcctgtg
     2581 tctatcttga tatgaaacca ttcatataaa agcgaggaaa tgatttgagc gatcccattc
     2641 atccctgcag aaaagacatt ggctggaatc aaaaaataat ttaaagcgac cgcagcggtc
     2701 aatccggtta tcaaaataac cactattttc tttaatcttt catttttatg atacgcagtc
     2761 ataattttac tcacaagcac ctctccccta ctttggtatt ccaaatgtaa gaataacagt
     2821 ttatctatta aaataaaata cttcgaactc aa
//
LOCUS       DAZKNO010000213         2842 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000213 DAZKNO010000000
VERSION     DAZKNO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.218"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..460)
                     /locus_tag="ACG8MY_002531"
     CDS             complement(<1..460)
                     /locus_tag="ACG8MY_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0431682.1"
                     /translation="MRCQQHLTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQM
                     TTVISVFGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLY
                     GIGDCIFTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
     gene            complement(500..1552)
                     /locus_tag="ACG8MY_002532"
     CDS             complement(500..1552)
                     /locus_tag="ACG8MY_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0431683.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1565..2503)
                     /locus_tag="ACG8MY_002533"
     CDS             complement(1565..2503)
                     /locus_tag="ACG8MY_002533"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0431684.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT          962 a          608 c          446 g          826 t
ORIGIN      
        1 tttgacgata aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc
       61 cagaacctgt attatggatc caagtcgata gattccaaga gctttcattc gtattgatac
      121 tgaataaccc agtaaaaata caatcaccta tcccatataa accaactgtt gcaacagcaa
      181 gtattgcaag tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa
      241 caaaaaaaag tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta
      301 cgtcaccaaa cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa
      361 ggatatacgg tgtcgataaa tcactaatca cacctaataa taaaaaataa aaaccatatt
      421 ttggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
      481 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
      541 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
      601 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
      661 gtccgaccac accgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
      721 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
      781 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
      841 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
      901 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
      961 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     1021 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     1081 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     1141 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     1201 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     1261 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     1321 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctaatt
     1381 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     1441 aaatagcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     1501 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     1561 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     1621 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     1681 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     1741 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     1801 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     1861 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     1921 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     1981 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     2041 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     2101 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     2161 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     2221 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     2281 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     2341 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     2401 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc attaacccaa
     2461 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     2521 tctttctatt atacttattt gagaatcatt tcacaacctt gttttgttat tcttctctta
     2581 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     2641 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     2701 tagaaaaaac ctcactcctc tgattataaa agaggggaca ttaacctcgt gtatactaaa
     2761 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     2821 tgttgcgcaa cacagacgga tg
//
LOCUS       DAZKNO010000214         2819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000214 DAZKNO010000000
VERSION     DAZKNO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.219"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..1489
                     /locus_tag="ACG8MY_002534"
     CDS             98..1489
                     /locus_tag="ACG8MY_002534"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0431685.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            1548..2357
                     /locus_tag="ACG8MY_002535"
     CDS             1548..2357
                     /locus_tag="ACG8MY_002535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0431686.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            2329..>2819
                     /locus_tag="ACG8MY_002536"
     CDS             2329..>2819
                     /locus_tag="ACG8MY_002536"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0431687.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYV"
BASE COUNT          979 a          398 c          551 g          891 t
ORIGIN      
        1 tcttttaatt tattttacaa tttggttatt cactaggttt ttaaactttg ctatggtaag
       61 atgatagagg cattatttta tggttaggag tgtatatatg aataaaaatg gggagtggaa
      121 tgcggcacgt aggatattca ttatcatcat ggatgtactt gtattgaatg tgtcgatcta
      181 tcttagcttt ttgctgaagt tccaaggaga agtcccagca agaaacttag agacctttca
      241 gcattcagca gtttttattt ccattatctt tattgcgttg aatattttgt taggagctta
      301 tgtattctat agccgagtga tcagtgatat cgtcttcatt actgttattg gacaggtgct
      361 gatagcattg gggatcatgg tagttacttt tgctggacgt tggtttgcat ttccgcgtgg
      421 cgtcatcgtg ctctcgttta tacttggcac gatattactt agcgtttatc ggatagttgt
      481 ttataaacta tacttaagag tcagtcgtga taagaaagtc cttatcgtag gtttggagaa
      541 agatgtcgca ccagctatcc aaaactttca atctaagaaa aacaacaaac ataaagttga
      601 agcagtagtg atcgacaagt attacgaaaa cacaaaaaaa ttgattgatg atatcgatat
      661 tgtctacttg gcttctaaaa tcccagaaga cgaaaaaatc aatatttatc aattagtaac
      721 taaaaaagag aagaagctat ttttaaatac aacttttgaa aatttaacga tgatcaatcc
      781 gaacatcatg aatttcgaag atgaaagtat cattgaagct tccggtttca aaattcagcc
      841 ggaatatgaa ttattcaaac gtctttttga tatcatcgtt tcgcttattt tactgattct
      901 tgcttcacct tttatgctaa tcacagcgat tttggtaaaa acaacttcac ctggaccaat
      961 tatttataaa caagtacgta ttacgaaaaa tcaaaaggaa ttttcaatct ataaatttcg
     1021 ttccatgagt gtaacagctg aagcaaaatc aggaccggtc ttggcaaaga gcaatgatgc
     1081 acgtgtgaca ccagtaggca aatttatccg tgccgtacgt tttgacgaat tacctcaaat
     1141 ctttaatgta ttgaaaggcg atatgtcgat cgtcggacca cgtccagaaa gaccattttt
     1201 cgttgatcag ttcaatgaag aaaatcctta ttattattta agacacaatg tacgtgcagg
     1261 gattaccgga tatgcacaag tatacggaaa atatgtatca gattataata gtaaactacg
     1321 ttttgatttg ttgtatatca aaaagtattc cttactatta gatttgaaga ttttacttca
     1381 aacaatcaaa atcttatttg ataagatgtc ctcacaagga ctagatgaag atgaaaatat
     1441 ccgttcgtta gcagaaatcg acttttcaca agagaaaatt tatcgctaga tgggaaaaat
     1501 tttgagcaaa gaagtaggag agaacattgg aaaataaaaa tataagtatg ctgctttctt
     1561 tatatgataa agaaaaacca gagtatttaa aggaagcatt aactagtatt tttgagcaaa
     1621 cagtaatgcc aaaagaaatt gtattagttt atgacggccc tataaatgaa aaacttcaag
     1681 tagttgtcac ttatttccaa caaaaagatc ctcacttgtt tacagttgta aaattagaaa
     1741 aaaatcaagg attggggatt gctttagcaa ttggattaga acatacgaaa aatcgcttag
     1801 tagccagaat ggatacagat gatatcatgg aaccccaacg attagaaaaa caattagaga
     1861 tcttccaaaa agatccagaa atagcgattg ttgggagtaa tattgaggaa tttactggca
     1921 cgcttagcaa tgttatcgga agaaggattg tacctgaata taatgatgaa atccgtgtgt
     1981 tttcgaaaag aagaaatccc tttaatcata tgacagtcat gtttgataaa aatgctattt
     2041 tagaagtggg aaactatcgc ccattaattg gatttgaaga ttattatttg tgggtaagac
     2101 tgttgaaggc gggatataaa gggtataata tccaagagat tctagtccat gctagagcag
     2161 gtgctgacat gtatgctaga cgtggaggaa gtaaatatct tttaccagga attaaaggcc
     2221 gatatcgcgt atggaaagat ggtctaggat cactaaaaga ttttctgata gttatttgcg
     2281 gacatcttgt aattagttta ttaccgaata aaattagggg aaaagtatat gaatcaaaac
     2341 ttagaaaaac aaactaaact ttctataatc ataccttttt acaatgcaaa aaatacttta
     2401 aaaagagctg tagacagcgt cttaacgcaa ggttatccca atattgaaat cttgttaatt
     2461 gatgatggat ctacagatgg tagtagtgac attggtgacg aaattgctaa tctagagagt
     2521 aaagtagcat gttatcactt ggaaaacgct ggtccatcag ttgctaggaa ttgtgggata
     2581 aaagaagcaa ctggaaattt ggtaggattt ttagatagtg acgattattt tttacctgat
     2641 tgttttaata atgttttgaa agactaccat aggaattacc ccgatatcat tgcttttgga
     2701 ttaagtaaag gaaaaaatat agaaaatgca aatttatttg ttcccgaaaa gaagattggt
     2761 tgttcaagtg aagaagcaat tgagcaaatg tttagaagta aggcagtgga tttttacgt
//
LOCUS       DAZKNO010000215         2805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000215 DAZKNO010000000
VERSION     DAZKNO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.220"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..188)
                     /locus_tag="ACG8MY_002537"
     CDS             complement(<1..188)
                     /locus_tag="ACG8MY_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431688.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
     gene            complement(335..670)
                     /locus_tag="ACG8MY_002538"
                     /pseudo
     CDS             complement(335..670)
                     /locus_tag="ACG8MY_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(682..2274)
                     /gene="mobP2"
                     /locus_tag="ACG8MY_002539"
     CDS             complement(682..2274)
                     /gene="mobP2"
                     /locus_tag="ACG8MY_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0431689.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDQEFSETSNEAQSLMGNGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYVVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYRNLPEKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKNLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            complement(2294..2716)
                     /locus_tag="ACG8MY_002540"
     CDS             complement(2294..2716)
                     /locus_tag="ACG8MY_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431690.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKSINESVHARVDPFDPTKFLKR"
BASE COUNT          703 a          560 c          419 g         1123 t
ORIGIN      
        1 aatggataat cttctgccgc atcgacgaac gtcttcgtag tcagatttaa cgttaaaata
       61 tcgccacttt gataagcttt tgcagctttg tctgtgacta caaaagctct gcctgtttga
      121 atttctgtca caaaaacgag attgtctctg cttttttcaa caacaactcc aatagttgaa
      181 taagccattt ctgcttcctc ccttctcttt cttttgatat aatgtagtcg gaaatcgcac
      241 cactcaatga attggcggaa aacaattcag tgaaaaaaaa gagtcctgga caatcacgtt
      301 caggactctt ttacttcttt tattcactta ataactaaca ctctgtgagt gaaatagact
      361 cttttatgct tttacatgga aagttattga ttccctcttc attcatttcc caagtagtat
      421 aataaggtgt taacttggcc tcatattctt gaatgataaa atatttttca ccgttcacat
      481 gattacaaca atacacacca gtagctgggt actcaacgac taaaaattaa tttggatcaa
      541 tctgttcgct tctcaacatt tctcttatct gaaacaaatg ctctttttcc aaacaatcgg
      601 gaagttgttc gattcgttct ttttttagtg atccttctat gactttccat tgattcaaat
      661 atcgtttcat ttctcttcct cctactgtct ttcttgttcc atttgaattc ttcgttgttg
      721 ctcttcgtat gctttacttg cgtggtattt ttcttcatgg gcattaatcg ttcgatttaa
      781 ttgacgcaaa ctccgctgcg tgacaactct ttgttggaat tgtttcctgc gcttcatgaa
      841 ttcttcgtaa ctatatttgt attcattcgt tggcaattgt ttaagcgttc gaaaggatcg
      901 ttcattgtaa atagattgca tctgatcaac actttctatt aatggtttta tttgactatc
      961 ctgtctcttc agctctgtta ggatggcatt tcctgtgcgc ttttttaaat caaagagagc
     1021 atttttctct gcatcctttg attctctctt gctttcccca taaagatttt ttgcttcttc
     1081 tttcaatgct tttgaaatct gtatatattc ttggtattca ggttcttttg ttaacacata
     1141 ttgggttaac tcgtcgagtt tctctcttgt gtatggtgaa agtttggttc gattttcatc
     1201 cccataattc cattccgaac gtttttcagg taagtttcta tatacttcat ttaataagtt
     1261 cattgcgtgt cgatcataac gtactggctc ttttaccact tttgttaagc tgttacgcaa
     1321 atcacttttt cgtgtcaata aatttcgttt ctccatacca atcattcgat ccttttcata
     1381 agagaaacgt tctaactcat gcacataatt ggttttcatt gcgtctaatg ttgcttgcct
     1441 tctctttcct ttcggtacaa cataactcac accattttca acctcagtgt aaggtttacg
     1501 cgtattttct ttttcaacaa ctgcaaaatg aatatggata tgtttagtgt tacggtgaat
     1561 cgttgccatc cagtagcctg tttcgttcag ttgctctttc tcgaataatg tctccatcat
     1621 ttttcttcca gctgctttta acccttcttc atctaaagta tctgttttgg gatcgtacaa
     1681 tcctattttt tcaagatacg aattatcatg agagacaaca tcttgataaa tgattgagcc
     1741 tttttgttgt cctctttgga agatttgctt aatctccttc agatcacttc ctttcacttc
     1801 gtctgaagcc atagaaaaaa ctccaggaag gtttcctgaa gcttttttgt gtggctctgt
     1861 ttgatatttt tttattgctt ggttcaagcg ttggtattct ttttgttccc tttgcgtcag
     1921 ttctttcttt tgttctaacg catagtgacg ggtcatataa gaaagatatt tggtaaaatc
     1981 ttgttcctct aacttgtcaa agttgattcc atttcccatc aaagattgag cttcattcga
     2041 cgtttcgctg aattcttgat ccgtcttact ctccgaaaaa gtttctcctt caggaatata
     2101 aaaatcctct aaagcattct tgattcgttg cagttcctgt tcttcttcag gcgtacgtcg
     2161 ttccatttct tccagcgctt tttctcttgt catataatca atgtatgttg aaaaagattt
     2221 tttattgggc aaggtaaatt gacaagtgag aatgactgca ggcgatttat tcatcatgtt
     2281 ccccttctac tttttatctt tttaaaaact ttgttggatc aaacgggtct actctggcat
     2341 gaacagattc atttattgat ttcttgattt taggagaata cttcttcgtt tgattcgctt
     2401 ttattttccc ttccacacgc tcttttgctt cttggtaaac agttgaattt gtaccaatta
     2461 aaatccctgt atctttcaaa tgaagatccg caaactctga aaccatctcg gttaaaatag
     2521 aaatttcttt tcccattgca ttcaatttaa tttgaattgg atttattttt tcttcgattt
     2581 ttggatcata catcatacac aaatgtctga ctgcttcact ataattagat atatcttcat
     2641 gagtagaaat tatttcatca agtacttcga tatgcatatc gtaaagatat acatgttttc
     2701 ttgttgcttt tgccaaaatt gatttcctcc ttttataaat tcacaatact atttttatta
     2761 ttcaattaac aaaccaatta attgattatt tgttgcaata tattc
//
LOCUS       DAZKNO010000216         2679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000216 DAZKNO010000000
VERSION     DAZKNO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.222"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..518)
                     /locus_tag="ACG8MY_002541"
     CDS             complement(<1..518)
                     /locus_tag="ACG8MY_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0431691.1"
                     /translation="MNVSEWVKSIDPNSLVSEQDVRTKIAVPLIKLLGYDEKYYADEF
                     PIYSYSGRKKNPTKFIDILCFSSDEWNLNRDLSSWPWVQDHSLISIELKKPSESLEDA
                     TGQAMFYSMNARSPFYIVLNGIDIKILKMIDFFSDTVIFEGPIENIAENWVDIYNTIS
                     FKKLSKETKEMG"
     gene            complement(660..905)
                     /locus_tag="ACG8MY_002542"
     CDS             complement(660..905)
                     /locus_tag="ACG8MY_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0431692.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRLRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIRDILQVPYEDLLQ"
     gene            1011..1784
                     /locus_tag="ACG8MY_002543"
     CDS             1011..1784
                     /locus_tag="ACG8MY_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431693.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTSSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETEFTEKILSIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKANIEKGALAQLYTEKVEQFFEAGSNKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLQKIKAYDRPLNLTLNNGSLSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(1837..2151)
                     /locus_tag="ACG8MY_002544"
     CDS             complement(1837..2151)
                     /locus_tag="ACG8MY_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431694.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT          904 a          472 c          352 g          951 t
ORIGIN      
        1 tcacccatct ctttggtttc tttcgatagt tttttaaaag aaattgtatt ataaatatca
       61 acccaatttt cagcaatatt ttcgataggg ccttcaaaaa taactgtatc tgaaaaaaaa
      121 tcaatcatct tcaaaatttt tatatcaata ccgttcaaaa caatataaaa aggacttctt
      181 gcattcattg agtaaaacat tgcttgcccg gtagcatctt cgagactctc agaaggtttt
      241 tttagctcaa ttgaaatcaa agaatgatcc tgaacccacg gccaggatga taaatcgcga
      301 tttaaattcc attcatctga actaaaacac aaaatgtcaa taaattttgt tggattcttt
      361 tttcttccag aatagctata gattggaaat tcatcagcat aatacttttc gtcataccct
      421 aataatttaa ttaaaggaac tgcaatctta gttcttacat cttgttctga caccaaagag
      481 ttaggatcta tactttttac ccattcactt acattcacta aaaaacctcc ttctattcaa
      541 aacacacgtt atacaaaagt ataccgtatc actacatagc tcatttatga ttatatcagt
      601 atttctttgt tatccataaa ggtgtaacaa tagattatta tactaaagac taaaagtagt
      661 cattgaagca aatcttcata aggcacctgt agaatatccc taatggcttt caattgatct
      721 aacttaatgt gttgcctgcc gccttcaatc ttaaccaatg tttctctagt tatagcgatt
      781 ttcctgagtt gtaggtctct aatcagctcg gtttgaccaa ttcctttttc taatcggagt
      841 cgtctaatat tgctaccgat tacattttct ttatcaataa ttttttcctc tttcaagaat
      901 agcacacctt ttggactaaa attagtcctt tttcttatta atcttaaaga tttggtatgc
      961 tacaatggga ctagtattag tccacattca atttttaagg aggaattttt ttgaagaaat
     1021 ttgcaatcat tctaacctgc gctttactca tcggaactat aagtgggtgt accacttctg
     1081 acacaagttc tacaagtacg gaaactagta caacaagctc agaaaagaat tacccaatac
     1141 atttttttcc ggttccagat ggatttgaag cttattatca tgataaaaag ctagatgtcc
     1201 ctttatcaga aactgaattt actgaaaaga ttttgtcaat tgaaattgaa agaaatcaag
     1261 acgatataaa actaaaagct gatcttccct tactgaacag cacacaaatt gatagacatg
     1321 aaaatatcaa agctaatatt gaaaaaggag cgttagcaca actttatact gaaaaagttg
     1381 aacagttttt tgaggctggt tccaataaag acttttcaaa aataagcgat tattcggata
     1441 actttttcaa tgaaacccaa gatgaaatca cgaactcaac ttttatggct gaagatatgt
     1501 ataatatgac gttacaaaaa ataaaagctt acgaccgccc attaaatctg acactaaata
     1561 atggatcgct ctcttttgta ttagcaggtt acgttgacta tgaatataca gcagattatt
     1621 tggagacgcc ccttcaacac gataataacg atattaattt tagatttatc tacaattcaa
     1681 aaagtaaaac atggttagtt gatcaattga gcactataaa tacgggattt catgaattac
     1741 ctaagcctga caatgaaact gctttggtca ttaaaacatt ctaaaaaaga aacgttgaag
     1801 gaataattat tctttcaacg tttcttttat tgtttttcag tttacggtct tacctgagcg
     1861 aataatctga tttctaaatg attggaaaac ttcttgtggt tgataataat ctgtatctat
     1921 ttcaaatgtt tctgagaata attcattgaa tttttctact gaaatgatga ttttaccatt
     1981 ctcctctcga ctgttttcta ttatttctgc ctctaattca aataaatcta gtgacgaact
     2041 aaaatcataa atcctgtcat attgaacaaa aaaatcaaga aaaagttgct caaactcttc
     2101 cttatttatc actagcgttt caagcggaac aactttattt tcatcagcca ttcttccaat
     2161 ttcctcactt tcattttcaa ttgtccattg ttgcggacgt atcagatttt ctctatcatt
     2221 tttcagtgta tagatcactt gtaatacaca acaatgcttt gtaaacttat ttttttagtt
     2281 agtcgaatct ttttaaatgt gcgtttcata ccatgcgctt gatatatttt ctcctatttt
     2341 ttaaatcgtt tatcacaact tttatcgttt tttcttctga aaaaaatctt gtttacatcg
     2401 ttacatagtt tccaaaaaga tacattacta gtagaaacaa caagattaat tttacttccc
     2461 actgaaatgg gaagtatatt ttaaatattt tttctccttc aacttacact ataaagtatc
     2521 ttaatcacac gatatgctag ccactaaatt cattatattt gattcctttt ttaagataat
     2581 gtagcatctt tttatccaat aaagcaattg tttttaagga taaatttctt aagatggtac
     2641 atttcaaaat ggcatttgac atatgaaaca aaaaagaaa
//
LOCUS       DAZKNO010000217         2676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000217 DAZKNO010000000
VERSION     DAZKNO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.223"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..465
                     /locus_tag="ACG8MY_002545"
     CDS             19..465
                     /locus_tag="ACG8MY_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0431695.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            738..2090
                     /locus_tag="ACG8MY_002546"
     CDS             738..2090
                     /locus_tag="ACG8MY_002546"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0431696.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            2261..2548
                     /locus_tag="ACG8MY_002547"
     CDS             2261..2548
                     /locus_tag="ACG8MY_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0431697.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          937 a          446 c          534 g          759 t
ORIGIN      
        1 acaacgtggg gtgtttacat ggaaaataag ttagtcgaag aggttatcga aaagttaaaa
       61 aaagagaaca ttcgaatcac acctcaacgg tacgcggttt tagaatattt gattgaacat
      121 cattcccatc caacggctga tgagatttat aaagcacttg aacatcggtt tccaaatatg
      181 agtgttgcga ctgtttacaa taaccttcgc ttatttactt cgattggatt tgttcaagaa
      241 atgaagtacg gcgatgcttc tagccgattt gatttcagtt caaagcgtca ttatcatgta
      301 atttgtgaaa actgcggtaa gattgttgat tttcattacc cagggcttga agatatagaa
      361 atggctgcaa gcagactgac tgattttgag attaatgaac atcggttgga attgtatgga
      421 ctctgtccgg attgtaaaga gtcagcaaaa aaggcgcaaa tgtaaaaaca gtttctacaa
      481 gtgttatacc taaaaaaagc tagctgtgta aaaaaaacgc tagctttttt atttttttgt
      541 cttttattca caatcttaga tataaaagtg aatattatta aaataagaat cttgtagata
      601 ttgggttctt atgcttgaaa aggaatgaaa ctacaaacaa acctttgtga attcacaagc
      661 attaaactgt tgtatccgtt ttatttctgt tatagtagtt ttgtcgaagg acaaaatgtt
      721 aatcggaggg accataaatg aaagtagtag taattggctg tactcatgca ggtactgcag
      781 ctgtaaaaag tattttaaaa aatcatccag aagcagaagt gacagtatat gaaagaaatg
      841 acaatatttc tttcttatct tgcggtatcg cactttatgt cggcggagta gtaaaggacc
      901 cagcaggctt gttctactca aatcctgaag aattatcttc attaggagca acagttaaaa
      961 tggaacacga tgtaacagac gtcgatacag aaaataaaac agtaacggca aaagatctga
     1021 aaacaggaga aacagaaacg gtttcttatg ataaattagt gatgacaact ggttcatggc
     1081 ctattatccc accgattagt ggaatcgatg cgaaaaatgt actcctatgc aaaaactaca
     1141 atcaagcaaa tgaaattatt gcacaagcga aagaagcaaa acgtgtagta attgttggtg
     1201 gtggatatat cggtattgaa ttagtagaag cttttgtcga atctggaaaa caagtgacat
     1261 tgattgatgg attagatcgc atcttgaata aatatctaga taaaccattc acagatatct
     1321 tagaaaaaga attgacagac cgcggtgtta ctcttgcgct aggcgaaaat gttactgaat
     1381 ttatcacaga tgaagaagga aaagtgaaac aagtagctac accaacagat acatttgata
     1441 cagatatggt cattttatgt gtgggcttcc gtccaaatac aaaactgatc gaaggaaaag
     1501 tagatacact gccaaacggg gcaatcaaag tgaatgaata catgcagaca agtaatccag
     1561 acatttttgc agctggagac tctgcagtag tcaactacaa tccaagtggc acgcaaaact
     1621 atattccttt ggcaacaaat gccgttcgtc aaggtttgct tgtgggaaat aacttgacag
     1681 atcataaaat ggcgtatcgt ggtactcaag gaacttcagg actttaccta tttggctgga
     1741 cgatcggttc gacaggtgtg accaaagaaa gcgcaacttt gaacgatcta gaagtccaag
     1801 cgactgtatt tgaagataat tatcgtccag agttcatgcc aactactgaa aaagtaatga
     1861 tggagctagt ctatgaaaaa ggaacgaatc gtatcgtagg tgctcagttc atgtccaaat
     1921 acgacattac acaatcagca aataccatgt ctttagctgt tcaaaataaa atgactgtag
     1981 aagatctcgc actttcagat ttcttcttcc aaccacattt tgaccgccca tggaattact
     2041 tgaacctact tgcacaagca gcgctaggcg aaatcgaaac tgcacaataa agaaaaaggg
     2101 ttgtgacaca attttgtcac gaccctctgt ctttttttgt gaaaagtaca attagttgtc
     2161 tttcttcgaa ttttttgtca caataagcag caaagataaa gctgttacat aaaagaacaa
     2221 gcagctttat ttgattttga aaagaaaggt gagaaagaag atgaataaag aatattttgt
     2281 ccaacaagat gcatttttag aagattataa aagtgcttcg aagaaattaa cgatcaatat
     2341 tgatgagttg gcagatcaag tactctctca gttgaatgaa acgcaagagg aatattacac
     2401 gatgccagca aatgacgcca aagataatgt ttctcatcgt tttcctttca gtagacgcat
     2461 ctactcagat gaaaatgacg gcacattgaa tacagagtac acatatgaag gtcagccata
     2521 tgtgctagaa actgatgatg atctttaaag acagtttttg aaaaggccgc tcatccttaa
     2581 gtgatgacaa aaaatcatca caattaccga tgactgtctc tttactatct tttaatcaga
     2641 aaaaggggtt atgacaagct ttgtcaaccc cttttt
//
LOCUS       DAZKNO010000218         2670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000218 DAZKNO010000000
VERSION     DAZKNO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.224"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..486
                     /locus_tag="ACG8MY_002548"
     CDS             142..486
                     /locus_tag="ACG8MY_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0431698.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            487..906
                     /locus_tag="ACG8MY_002549"
     CDS             487..906
                     /locus_tag="ACG8MY_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFQ0431699.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(998..1348)
                     /locus_tag="ACG8MY_002550"
     CDS             complement(998..1348)
                     /locus_tag="ACG8MY_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431700.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1314..2645)
                     /locus_tag="ACG8MY_002551"
     CDS             complement(1314..2645)
                     /locus_tag="ACG8MY_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0431701.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT          987 a          487 c          514 g          682 t
ORIGIN      
        1 aagcatatcg actgaataaa ctaagcattg gacaggctga aagactacat ttaaaatgaa
       61 atgcgaagaa acaatagaaa ataaagttgc cttagaacca tagttgaatc acctaacagt
      121 cgtgaaggga gaataagaga aatggcagaa aaacaaaccg caggacgaga tcaattagga
      181 gattttgcac caaaatttgc agaattaaat gatgatgttt tatttggaca agtatggtcc
      241 agagaagaat tatctgctca tcagcgtagt atcatcacca tatctgcatt aatttcagga
      301 ggaaattttg aacaattgcc agctcatctg aagattggca agcaaaatgg cattactagt
      361 gaagaaatag cagaaatcat tacccattta gctttttatg tagaatggcc aaaagcttgg
      421 tcagctttta gtttagcaaa agaaatctat ttagaagaaa atagcgaaga taagaaagag
      481 gcgtaaatga tgacaaaaca cgaagaaata cacaatggcg tgatttttcc agcaggtgaa
      541 aaaaacgaag cttacgctca agtatttacc gggcaaagct atctgaatat gttagtgaat
      601 gatcccgaag tttctgttgg tgtaggtaat gtaacttttg aaccaggttg tcgcaatcac
      661 tggcatgtcc atacagacgg ctatcaaatc ttacttgtga ccggaggaga aggctggtat
      721 caggaagaag gaaagccagc aagacgatta atggctggag atgtcgtcgt aacgcacaaa
      781 gggattaaac attggcatgg tgcgacaaaa gacagttggt ttgaacattt ggcgatcact
      841 gctggtaatt cagagttttt agaacctgta acagatgaag tgtatgaagg attggaaaat
      901 caataatgtc aagcttgaac agctcctgaa agtagatttt aatcgaactt tcaggagctg
      961 cttttttgat tcagtctttt ataaatagaa aatagggtta ataataggaa tgagaagcac
     1021 gtcgaaagat cttaatattg ataatcggct gggcatacat gatttccacg tcatcaatca
     1081 aactacctaa cagatgataa tcttcccgat catgtcgggg attacctaat agttcttgat
     1141 agtagatttc catactaggg tcccgaggat tgttcagcaa atcagcaagt acatctaaat
     1201 cttctggctg agtttctgcc gtaccctgta tctctatgaa aagcccttcc tcagcaaatt
     1261 ctactccaat acgtatttca gttatttcat gggaatataa ataagtaaga agctcatcga
     1321 tgattctttt tgataattct ttttccaagt gtcttttttt cctttctgac agcggtaaag
     1381 ttatctagta tcggtacaag gatacctgca acgaaaccag ttgaaaaacc actgttatat
     1441 agatttagtc ctccgtgcaa agtgctgacg ttatggacaa gagcaatatg tagcacacca
     1501 gctattacac cgtaaaaact gccgtaaaaa ccgcttatgg gcgcaagacc tgtcccaaac
     1561 acagctgcaa caagcatgcc agtactggtg atctcatgaa aagttccaaa caaagaagca
     1621 ataaatattc cgactagtat cggaaaactg ttttttaaat gacaaccaaa agcactgaaa
     1681 cccaccgcag ataaaatagc accgatgaca ggtccattta actgaccgcc aaccagcaaa
     1741 acataagaag acagcatcag accaactaag cccatgttga tcaaagtagc accaatacca
     1801 gaattagcaa tgaaatcagt tgtcaatttt ccagatgaat cgaaaatttc tcgaatcccc
     1861 gaaagccgaa aggaattata atagaaacca attccgactg tcagcgaaaa aaagccaaaa
     1921 ataatccaaa tgagaggaaa gtgatattca ttgaaaacca atgtattttc gacgattgaa
     1981 tagctgaata acctcaaaac agcagtaaaa gtcatcgcaa tcaacccaga ggtgaatcca
     2041 atattataaa gtgtgaatcc acgatggaaa actaaagttt gagcagctaa gggcggaaga
     2101 agtataccaa ttgctatgcc agctaaattc cccaataaaa tacctatcag taaaggaaaa
     2161 cgcataccaa aagtgatata actaacgacc ggtgataata cgctaccaaa cagtccgatc
     2221 atgatgtact gagaaaaagg tttattgaca aatacagcgt atagatacac acctaaaatg
     2281 attgagatag aattataaag gtttttgcca aaaaaagaga aaccggtaac tgttagaatc
     2341 gaagcaaata agggaccgtt taaaatcacc ttgtgccgat aagctaaaag cacagaaagt
     2401 aaggtcaaaa tccctacatt tatgaatgcg cttccgaaaa cacctaaagc aaaataatct
     2461 gtcaaaagat tgcttggcga ggtaagaata gttagcaatc cactccaatt atcagtagga
     2521 gtattagaaa gcaagccaat aaaaagaaaa aaaacacctg taaaaccaag ataaaaatac
     2581 tttcttttct gtgaaacgat tcgtttttga ggatgaatat aaataacgtc agtttcgttt
     2641 tccattacac accttctttt tcattttctt
//
LOCUS       DAZKNO010000219         2643 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000219 DAZKNO010000000
VERSION     DAZKNO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.225"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..614)
                     /locus_tag="ACG8MY_002552"
     CDS             complement(<1..614)
                     /locus_tag="ACG8MY_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431702.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQV"
     gene            655..1347
                     /locus_tag="ACG8MY_002553"
     CDS             655..1347
                     /locus_tag="ACG8MY_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0431703.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
     gene            1907..2590
                     /locus_tag="ACG8MY_002554"
     CDS             1907..2590
                     /locus_tag="ACG8MY_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFQ0431704.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKRFDEPKVVVTDKAPSITSAFKKLKEYGGFYQGTEHRTI
                     KYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTE
                     IKVLLGIPA"
BASE COUNT          847 a          480 c          489 g          827 t
ORIGIN      
        1 acttgccaag gattaaatga aaacgtactt ggcacgacgt gatctggatc ataggtctcg
       61 atgatttttt cgtattgttc cttgtagtca ttatcaactt ttgcttgggg gatcacttta
      121 cttgcttcat gcaaaaagtg ttctgaacca cgccccattg cctgtccccg aacagtatgt
      181 ttatccaacg caatatcagg aatttctgga atatatccca tagcgaaact cttgataaca
      241 atgttcttaa gtaaatccga tgaacgatct ttttcagatt ggcataggta acgaatcgcg
      301 tggaaaaaat acatcgcacg atcggattca ttgtattgga attctttgcg catggcgttt
      361 aagttattga tcatgatggc ggccatagga ttgcccatgc ccacatcctc aacactgatc
      421 gccaatagcc ttctccagag tttctcttca aattgtggtg aggtgatata catttcataa
      481 gcaaattcac atgcttcttt ttcttttcct cgacgaatcg atttctgtaa tgctgagata
      541 gcttcatctc cacgtaagcc gttacgggta gtcatttttg accacggatc ttgaataaat
      601 ttcgtttctg tcataatata atactcctat tagataaaat ttgaggtgaa tcttatggat
      661 gttgaaatgc ttatagataa ataccaactg aatgactcag aagctgaagt cttacgattt
      721 atgaaaaaaa atcgtcagtc tttaaaaaat attgggattc gtgacgttgc caaagcatcg
      781 tatgtttcta ctgcaacgat tatcaatatg gcgaagaaaa ttggatattc cggttatagt
      841 gagttggtat ttgctctagg aaacagccaa tctgacatta cgatgccaag cacattctcc
      901 aatgaggaga aggaagcatt catacattta atgtctgcgt atcgcgataa acggattatg
      961 attcttggct caggtttttc gcaaaatcta gccaattatt tttcggaatc tcttaatcta
     1021 tatggatttc gtgcgacggc caatagtcat ttagaatttc ttagacagca agtgcaacaa
     1081 gatattctgc tttttattat ttccaattct ggtgatacag ttcgattagc cgaattagct
     1141 aaaattgcga acgatcactt tatcgatgtg atcgcatttg ttgggcaaaa aaattcaaaa
     1201 atcggtacgc tggcaacatt aaccattagc acggaaacct atattccaca aaaaatatct
     1261 gattaccaac caaatctttt ctttggcacg acactgaatc aatttgagct tttgttgagc
     1321 gcggcgctga aggctattta taaatagaag aacgctgtgg ccactgtttc ttcaaaatct
     1381 agtataaaga atcttcttta gaatgtaatt gtttcctgtg tgttctgaac atgtttagga
     1441 atttccaaac ttgtttagaa aactttctca ttccctccgc tgtagaattg aactcacata
     1501 aaaaacatct aaaccaaaat atctacaagc ttggaaagga taataggttg ttattaaaaa
     1561 taatttttat tcctgaacct gaataattca tagctttaat tctttggcac attttattga
     1621 aatatgtatc actaatatcc ctatcaactg attttgacta aaaagttact tcaaacattc
     1681 actgttcaaa aatattgagg aataaatttt gggtgtaagg cattttgagc atacttctcc
     1741 ctggataact tcccagtaaa aaaatcgtta gattgttgga ttagatccat tgactgcctc
     1801 gcctcaggcg agcaaagacc ctgtagaatt caggttctgt tgcaaagttt taaatctact
     1861 atcaaataag gtagaataat agaaaaagat agcaggagga atgacgatga atcattttaa
     1921 aggaaagcaa tttcagcagg atgtgattat tgtagccgtg ggctactatc ttcgttataa
     1981 ccttagctat cgtgaagttc aagaaatctt atatgatcgt ggcattaacg tttctcatac
     2041 aacaatttat cgttgggtgc aagaatatgg caaactactt tatcaaattt ggaaaaagaa
     2101 aaataaaaaa tccttttatt catggaaaat ggatgaaacg tacatcaaaa ttaaaggaaa
     2161 atggcattat ttgtatcgag ccatcgatgc agatggttta accttggata tttggttacg
     2221 taaaaaacgg gacacacaag cagcgtatgc ttttcttaag cggttagtga agcggtttga
     2281 tgaaccgaag gttgtagtca cagataaagc cccctctatt acaagtgcct ttaagaaact
     2341 aaaagaatac ggcggctttt atcaagggac agaacatcgt accattaaat acctgaataa
     2401 tttgattgaa caagaccatc gcccagtaaa gagacgcaat aaattctatc gaagtttacg
     2461 cactgcctct accacgatta aaggcatgga agccatccga ggattatata agaaaacccg
     2521 aaaagaaggc actctcttcg ggttttcggt ctgtactgaa atcaaggtat tattgggaat
     2581 cccagcttaa atcatagata ccgtaaggga ttttattctt tatttaaaac tttgcaacag
     2641 aac
//
LOCUS       DAZKNO010000220         2638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000220 DAZKNO010000000
VERSION     DAZKNO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.226"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(365..931)
                     /locus_tag="ACG8MY_002555"
     CDS             complement(365..931)
                     /locus_tag="ACG8MY_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0431705.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(931..1887)
                     /locus_tag="ACG8MY_002556"
     CDS             complement(931..1887)
                     /locus_tag="ACG8MY_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0431706.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1887..2543)
                     /locus_tag="ACG8MY_002557"
     CDS             complement(1887..2543)
                     /locus_tag="ACG8MY_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0431707.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          802 a          536 c          483 g          817 t
ORIGIN      
        1 ggcataattt gactgatatt aagttagcta ttgatatatg aatgacatac aaaaatataa
       61 aatcttttaa aaatgcttat atatcaatgt ttttgccatt cttttatata tctgttatat
      121 gtcaaaaaaa tgtgttcaat ttcaatttgt atttgaataa agaggttgtg aaaaagctgt
      181 cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgatttggtt
      241 ggacttatta ccgcagatat aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa
      301 cgctctaacc tgagtattag agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt
      361 ttatttatct ttttttctca atacaatata aaataggcgg ctggttcttt tggttgataa
      421 accgataatt cagcacatta tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca
      481 tatccagttc tttttctcca ccttcatgac catagtagac gacaaggatc aatcgtccgc
      541 gaggcacaag tcgtttcaat atttcttcca tagctgtgcg tgtagtctct ggcatagtga
      601 tgattgcttt atcacttttt gggagatacc ctagattgaa aatccctgct ttgagatttt
      661 gttgttcagc tatcacttct cctaacgttt cgtggccttg tagaaacaat gagacacgtt
      721 cttctaactg acgctgttct agcctttcac gagtagcatc gattgcttgt tgctggatat
      781 caaaactata gacatgacca gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc
      841 cattgcccat cgttgcatct acgacgtgat cccctggttg caagatttct tgcagtaatg
      901 tatggctgaa atgcaaagca gtttgcaaca tcagacgctc acctttttcc gtacatcata
      961 ttttccttgg aagctatctc tgcgaagaag ttcttcatca attgcattca agacttccca
     1021 ttttttcaag ctccacattg gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg
     1081 atggatgatg atctcttttg gtatcatttc cagctggtcg caaatgacat tgacatagtc
     1141 aggacggctc attaattgaa ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg
     1201 catcagatgc atcaaatgga gcttgattcc ttggatatct gaatctaaaa tggtgcgtct
     1261 gacattttct ttcatcattt ctaaactttc gcctggtaat ccgttgatca aatgcgtaca
     1321 aacacggata ttatgtttgc gtaatttagc cacgccatct aaataagttt gataatcatg
     1381 tgctcggttg atcagttttg aagtttcttc aaaagttgtt tgcaatccaa gttctaccca
     1441 taaatacatc cgctcgttca gttcagcaag gtaatcaact acttcatctg gcagacaatc
     1501 cggtcgtgta ccgactgata atcctacgac acctggtaaa ttgactactt gctcaaatcg
     1561 ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta
     1621 ttgttggaca tgaggccatt tttgatgcat catgtcgatt tctttctgga attggattgg
     1681 aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca gatacactgc aaaaagtaca
     1741 accgccatgg gcaacagtcc catctcgatt cggacagtca aatcctccat cgatcggcac
     1801 cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa ttccaagaat gatatctttt
     1861 atttccttct gaataaggaa aagtcattaa aagtccttct ttctgtaaga gttatctatt
     1921 tgttttcttt tttctctata cactggataa gtcaggcaca gccaagaaag actgagtaag
     1981 tagccgccga tgatatcagt aggaaaatga acgcccagat aaatgcggct tatcccgaca
     2041 cttactataa taaacatcat cagcaattga agcaatgtgc gtaaggttct tttttgaaca
     2101 aaagctggaa gcagaaatag taaagaaccg taaagaatca tactgcctgc tgcatgtcca
     2161 ctagggaaac tgtaacttgt ttcaactact aagtgcgcca atgaaggacg ctctcgcatg
     2221 aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta caataaatcc agaaacgaac
     2281 caaaatgttt ctgccctttt tttcttatac cacatccata aagccgcaca agcaaataaa
     2341 atgatgacgg taagaggatt accgaattta gtcataaaga gttggtagct gttcagatca
     2401 ggataagaag ctctgacaat gcctgtaaac cactgatcga aaccagaaag ccacgacaga
     2461 tggaacttta cgatccagcc caaaatgaaa aacaggacaa gaaagaagct accagccacc
     2521 tgaaatgatg ttttatattt catacaattc ctcctttgaa gcaatgactt attatagcat
     2581 ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa tgtatgattc ctctttga
//
LOCUS       DAZKNO010000221         2602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000221 DAZKNO010000000
VERSION     DAZKNO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.227"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     misc_binding    complement(83..289)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(359..2377)
                     /locus_tag="ACG8MY_002558"
     CDS             complement(359..2377)
                     /locus_tag="ACG8MY_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0431708.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
BASE COUNT          672 a          536 c          482 g          912 t
ORIGIN      
        1 ctgtttattt ttatttcctt ttgctttgtt ttctcaaact tcacaaaaga aataaaaaaa
       61 tcctataaaa agattgctct ttttatagga cgtaatctac gcggtaccac ctaattttac
      121 cttagtgaaa aggcctcgaa cgttgtaacg gccgtcaccg ccgcttgctt actcgggtat
      181 tcagcaaggg caactcaaag gcgagttcaa aagcatccgt gcacattctc accagccatg
      241 cgctctctga aacttcagtc tttttactac ttccttttca agtgttgtca tatttaagta
      301 gccttatagt aacaaatatt tttgtggaat tcaatcgtta tttcttcgaa cagctatttt
      361 atttagctac aatcaacgtt tgacctggat aaataaagtt gttcttaata ttgttccatt
      421 cgattagttg agccattgta attccatgac tgtcagcaac actccaaaca gattctccag
      481 atttcacagt ataggttttc gaaccagtaa ctgattgttg gtttgttgat tgagatgaac
      541 ctttcttcac aatcacttgc tgtcctggat agatgaaatt atttttgata ttattccatt
      601 cgattaactg atccatcgtg atatgatgtt tgttcgctac gctccaaact gactcgcccg
      661 ctttcacggt atacatcgta ttaccagtgg atgatgtact tgtcgtagca gacgtattcg
      721 gtgtatttgg tttcgctgca gaagtagttg aacctgtatt tgtatttgcc gaagttccac
      781 ctttaacgat caattgctgt cctggataaa tgaagttgtt tttgatatta ttccattcga
      841 ttaactgatc catcgtgatg ccgtttttat ttgccacgct ccaaacagat tcacctgctt
      901 tcacggtata agtgccttga gacgaactgc tgccagtatt aactgaactg ctgttatttg
      961 tattggttga cgaacctgta ttacctgacg tgctgtttgt tgatccactg tttgaaacag
     1021 tcagttgttg tcctgggtag ataaagttat tttggatatt gttccattgg attagctgag
     1081 ccatcgtaat tccgtattta tttgagacgc tccaaacaga gtctccggct ttaactgtat
     1141 atttcgttcc tgaagctgta ttagaagaac ttgagctgga cgaactagag ccacttgtat
     1201 tcccagtatt tgagccagta tttgctccag cattagaaga accgccattt gatacgatta
     1261 gctgttgtcc tggataaacg aaattgttct tgatattgtt ccattcaatc aattgagcca
     1321 tcgtaattcc gtgatcattt gagatcttcc aaacagaatc accagattga acagtgtatt
     1381 tcgcaccagt tgtttgtcct ccgtttgatg taccagcatt tcctgaagag ttggtattgt
     1441 taccggaatt attcgtcgaa gagccatttt cctggctacc tttgattgtc agtttttgtc
     1501 ctggataaat gaaattgttt tggatgttgt tccattggat caattgatcc attgtgatac
     1561 cgaatttatt tgagattccc caaacagaat caccagattg gacagtataa gtggtcgaag
     1621 ttgctgaacc tgagtttgta ttaccagaat tgcttgaacc gccagtacta cctgcacttc
     1681 cagtattatt gccacctgta gtcgctccac cgcctgcgtt tccgctagct ggtgtatcat
     1741 attgggtcaa tccgtaagtt gcgatgatat tatttaattt gcctgcgtaa ccaggatcag
     1801 tcgcataacg ccctgtcaaa caagctgtcg catctgtata ggatttcgtg ttgctcttcc
     1861 aagtaccggc atagtaataa ccgttgccaa atgaagtatt tctaagtaca taggcattgt
     1921 cattgaaaga ttcagcaaaa gaaggatatt gtcggaaagg ctccttttta tttacccatt
     1981 gattatttaa aaattcaagc gtatccatgt agacggtttg tccttgatag ctgcctttta
     2041 tcccaaaaag attataataa ggtgcttgag ataacgcact actgccccaa ccgctttcta
     2101 caactgcttg tgcgatcatc actgatgcat atagatcgtt ggcttgagcg attggttgag
     2161 cataagtagc aatttctgtg ataaatgccg ctgtattgat tcttgcgccg aactgaacag
     2221 gtgtttcatc cgcttgaaca ggaaccggct gtactaatgg ggcagcgaca gaacagccta
     2281 ccatcgcagt tcccacgatt gctgcccctc gttttgcttt tcgaaaacgt ttggtctgtt
     2341 ctaaacgtct ccgttctttt cttgcaatgt tttccatctt ctatcctcca acttcaattt
     2401 atctattatt aataattata acttctctta atatttatta aaagttattt aagagctttg
     2461 ttgttttttg taatcatatt gtaatttacc gtaatgaaca acttctgttc attttcaagt
     2521 tcttcctacg tctacgttct tgtttaaaag gatttcgaaa aacactgatt ttatttccct
     2581 aacatttcat ttgcattacc aa
//
LOCUS       DAZKNO010000222         2566 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000222 DAZKNO010000000
VERSION     DAZKNO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.228"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..653
                     /locus_tag="ACG8MY_002559"
     CDS             87..653
                     /locus_tag="ACG8MY_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0431709.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            798..885
                     /locus_tag="ACG8MY_002560"
     tRNA            798..885
                     /locus_tag="ACG8MY_002560"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:830..832,aa:Ser,seq:cga)
     gene            1221..2195
                     /gene="bsh"
                     /locus_tag="ACG8MY_002561"
     CDS             1221..2195
                     /gene="bsh"
                     /locus_tag="ACG8MY_002561"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0431710.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          886 a          380 c          495 g          805 t
ORIGIN      
        1 gttttgagta aaatgtttgc aaaaaatgct aaaaataggg tacaataaaa caacatatac
       61 tgcagtaaag aagtggggga gttttaatga gaagaaaagt ctataccaag gatcaaattc
      121 taaaagctgc ttatgatgtt gttgcaaagg aagggtttaa aggatttact gcgagaaata
      181 ttgctaaaaa gatggggatt tcgacacagc cgatttatct ggaattcaaa aacatggaag
      241 atttaaaaaa tacgttgctg gattctatct ttgaacaact aaaagaagat gttttcccta
      301 ttgaacgtac tggtgataaa atcattgatc tcggtttgaa ctatatctac tttgcaaaaa
      361 agcatcatag tctttacatg gcattgtaca ttgatgaaca tggggacgga aagaaaatgc
      421 atgatttttc atacaattat ttccgtgaaa tagttaaaga agatgaaaga tacaatcaat
      481 tatcggatga atatgtcgat gctctgcata ctggtacatg ggtagcagtg actggcttag
      541 cgtcattgat gtcatcaaac atcatgcatc caacagaaga acaaatcatt gcactgatca
      601 aacggacaat tgactcgatt ttagcattag ataatcctga aataatcaga tagtgttcta
      661 tcagtatgaa tttgatcggt aagtccgttg gctgcaaact gtcaatggac ttttttgcta
      721 aaaaaagcga tttctgttat ttatctatta gaaataggct tgacatttcg tgaaaaaagt
      781 ttaaaaatag tacttacgga aggttggcag agtggtaatg caccggactc gaaatccggc
      841 gaaccggcta ataccggcgc gcaggttcaa atcctgtacc ttccttatat caacgattag
      901 aggttgtgaa gaagctgtcc tgcatcaagt aataagaacg gccaaacaaa aatagctttt
      961 cctatttttt cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttgt
     1021 tcggaataag gggctgtgac aagacttttg tcacagcccc ttattgttgt acttttaacc
     1081 tacaatattt tttgattccc cctgagtact attttttata aatgctagta aatcaatctt
     1141 ctttggcctt ttttttgata aaaaaaaata ctagatgtaa gataaaagtg taaaaacgat
     1201 ttgttttagg agggaaaacg atgtgtaccg caatcactta tgcaacgaaa gaccattatt
     1261 ttggaaggaa ttttgattat gagatgtctt ataatgaagt agtcactatt actccaagaa
     1321 attatcgttt cgattttaga aaggtaaaaa atttagataa acattatgca atgataggaa
     1381 tagcagctgg ggtatccaac tatccactct attatgaggc tacaaatgaa aaagggttga
     1441 gtatggcagg actgaatttt cctggcaatg ctgattataa agaattacaa gaaggaaaag
     1501 ataacgttgc accatttgag tttattccat ggatactagg gcaatgctca accatagacg
     1561 aagctaaaga attattggct actatcaatc tagtcaatat cgattttagt gaaaaactac
     1621 ccttgtcacc tttacactgg ttattagctg ataaagaaaa atcgatagta attgaaagta
     1681 tgaaagatgg acttcattta tatgataatc ctgtcggtgt gttgacaaat aatccgccat
     1741 ttgattatca attatttaat ctgaacaatt atcgctcgct atcaaatgga acaccagaaa
     1801 atcatttttc aaaccagatc agcttgaatg tttatagtcg tgggatggga ggattgggct
     1861 tacctggaga tctctcttct gtttctcgat ttgtaaaagc cacatttact aaaatgaatg
     1921 ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt ttttcatata ctaggctcag
     1981 tcgagcaaca aaaaggagta tgcgatactg gagaaggaaa atacgaatat acgatttatt
     2041 cttcttgctg taacgttgat aaagggattt attattatcg tacgtatgaa gacagtcaaa
     2101 ttactgctgt aaatataaat aaagaagact tggatcgtca tgaattggtc agttatccag
     2161 tcataaaaga gcaacagata aactatgtca attaattaat gaatagatcc gtttggtatc
     2221 atattttctg atacagtcat aattagacac gatctttagg tcgtgtcttt ttttgctgtt
     2281 ttttttggaa atagcatcca ctaatatttt tttaacacga agagaataag gtagcctcaa
     2341 aaaagaaaac atggtaaatt agatattggg actttatttt ctgtaaaaac agaaactttc
     2401 atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat attcgtccgc tatttattct
     2461 atgataagat gcatatgaat gaaataaaca gtaattttat attaggatgt gaaagttaaa
     2521 tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa aaaaac
//
LOCUS       DAZKNO010000223         2560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000223 DAZKNO010000000
VERSION     DAZKNO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.229"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..239)
                     /locus_tag="ACG8MY_002562"
     CDS             complement(54..239)
                     /locus_tag="ACG8MY_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431711.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            454..825
                     /locus_tag="ACG8MY_002563"
     CDS             454..825
                     /locus_tag="ACG8MY_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0431712.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(975..1047)
                     /locus_tag="ACG8MY_002564"
     tRNA            complement(975..1047)
                     /locus_tag="ACG8MY_002564"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1012..1014),aa:Gln,seq:ctg)
     gene            complement(1093..1521)
                     /locus_tag="ACG8MY_002565"
     CDS             complement(1093..1521)
                     /locus_tag="ACG8MY_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431713.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(1581..2339)
                     /locus_tag="ACG8MY_002566"
     CDS             complement(1581..2339)
                     /locus_tag="ACG8MY_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0431714.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          807 a          423 c          466 g          864 t
ORIGIN      
        1 ggacgatgca gcagcattgt cctcctttga ttttacaaaa gtgaaagttt tacttatttg
       61 aatagatttt taaatttgtt tttgacactg tcgatattta catcttttaa aagattttgg
      121 gctttctctt tataaggtcc taattgttct ttatgttctt cgataaatcg ttttgcgcca
      181 tcgaaattgc cgttcttaat catttccttg actttttgaa ataattcttc ttgattcatt
      241 ttgaacacat cctttgctat caacatgatt ttttacatgg acaaaacgag aaaaactgat
      301 ttatccacac gtcaataata tcaaataacg atcattttta ctaaaaaaac actcgattct
      361 cgcttctaaa gtaggagaaa aatctccaac ttgggacgga tggaatttct ttaactgacc
      421 catatactga atatataaca aggaggtaga aagatgaaag tgattaaata tttgattgga
      481 ctggcggcga ttagtggtat tttgctattt ggactgaaat attatactga aggaagttat
      541 ggagaaatcc ctggtatact tgatcagcta aatccattag ttgaaaaagg agaggtctat
      601 gtaaagacac aaaaaccaga ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa
      661 gctgctgatg ccaatgggaa agaacgaacg gttgaattta atggtctttc tgtgttaaaa
      721 gaaaatcatt atttgaaaat tagcaataaa ggagcacaca tagaaacgta tgaggaagtt
      781 cctagagaaa aagttccgga gaaagcatta gcaattattg ggtaaatcag tttttgtatt
      841 aagaatacgc ccgattttat tattcataat ttaattatgt aaactaaata caagtagtaa
      901 aaataataca ataatccccc tgcaaaaaga ttgttctttt acagggggtt cattttttca
      961 gcaagcacat caaattggga tggcagggtt cgaacctaca attgcttgat tcagagtcaa
     1021 gtgccttacc agtttggcta catcccattt attcaacaaa ataatcatag cactttccta
     1081 aaaaaacgca agttattttt cttgatcgga tagttgttgc tttactcgta aaaggcggcg
     1141 tactagtttc ttttctaatc cagcaggaag ggcaatcagt tctgctactg acaagacggt
     1201 gtccaataac tgatattctt tgacaggttc attcataata tgatagatgt ttgctaaacg
     1261 ttcaatatat tcaaagcgag tatactctgt tcgagcaatt agaaatttca ataaatcggt
     1321 ggctttctcg tactcttgat ctgcgtaata aacaagagat tgacgataca atttgatttc
     1381 cagttcattg agggtagcag gtctgcgacg ctcgttccac tcattaatag agactttacc
     1441 ttgaacattt cccttgcagt cacggacaaa aataatcgga atttcatttg gatccatttt
     1501 tttatgtgtg aatttttcca ttatctacgc tcccttcaaa ggtttggtat cttgagtata
     1561 agggattttt gtttgaatta ctagcttttc tccaaagtta gcatctgtcc acccattgca
     1621 gtgaaaatcg ccgaaaaatc gttgatcggt actgctcgat tttttacacc atagggatcg
     1681 ttgacataaa tattttcttt gtcatatcca gtgactacac aagcatgaac aagaggagtg
     1741 actcttactt ctccattatt tgtggtccaa gtcataaaat cagtttcatc tgggacttca
     1801 aaattgatag tcacagaagt ccagacagga tttcctttag atagtgcttt tttgatatca
     1861 tcgagttgag catcggtttt tggaaaagtg tgataatccg tttggacgat ttgctgagca
     1921 agggaggcaa ctgggggaac atagacgacc atagcccagt ctccgccact gatatcacca
     1981 acaaatcctt cattaggatc gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc
     2041 aattgctcag ctaactggtt tttagttgtt tcataaccat aaaaagataa aagcatagaa
     2101 agcgaagtga cttcacagcc attttctaaa gaaggagaat caaactggct ctctaatgga
     2161 acatctagta aaacttctgc tttctctttt gtctttttcg ttttttgtgt tgaacttttt
     2221 tgtgcagtgt tcattgtatg ttctggagaa tggatatgta ggtaataact tgtaccgctt
     2281 aataaaagag cagctgtgcc ccctagaaac aatagttttt tgaacgtttt tttcttcatt
     2341 tgaaatctcc cttttcagct gtttatcttt cctattataa atgtattttt attggaaatg
     2401 gaagtattat attttaatat gtaggaaata attagttttt aaggaatatg ttttaatttt
     2461 ttgaaaaaaa ttcttctaaa tacaacagct gttttaattg tgaaaacgaa aacagtacgc
     2521 tataatgcct gtaaggttga tttttaactg taaataaaaa
//
LOCUS       DAZKNO010000224         2558 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000224 DAZKNO010000000
VERSION     DAZKNO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.230"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..720
                     /locus_tag="ACG8MY_002567"
     CDS             64..720
                     /locus_tag="ACG8MY_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431715.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            720..2096
                     /locus_tag="ACG8MY_002568"
     CDS             720..2096
                     /locus_tag="ACG8MY_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0431716.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            2226..>2558
                     /locus_tag="ACG8MY_002569"
     CDS             2226..>2558
                     /locus_tag="ACG8MY_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0431717.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1016 a          307 c          456 g          779 t
ORIGIN      
        1 tcttcattaa tgttaatcaa gaagcctaaa gaaatcttat caaaaataag ttaggagaaa
       61 ataatggata tattagaaat aaagaatgta gcatatagtt atgcaaattc taaagaaaaa
      121 gttttgtcag gagtaaatca aaaatttgaa cttgggaagt tttatgcgat agtaggaaag
      181 tcaggaacag gaaaatctac acttctttcc ttacttgctg gacttgataa acctcaaaca
      241 ggaaaaatat tgtttaagaa tgaagatata caaaataaag gatatagtaa tcatagaaaa
      301 aataatatat ctttagtatt tcaaaactat aatttaatag attatttatc accaattgaa
      361 aatattagat tagtaaataa atcagcagat gaaagtatct tgtttgaact aggtttagat
      421 aaaaaacaaa taaaaagaaa tgttatgaaa ttatctggtg gacagcaaca aagagtagct
      481 attgctagag cattggtatc agatgctcca ataatactag ctgatgagcc tactggtaat
      541 ctagacagtg ttactgctgg agaaataatt aatatattaa agacattagc taaagataga
      601 aataaatgtg taatagttgt aacacatagt aaggaagtag cagattctgc ggatatcatt
      661 ttagaactaa gtggtaaaaa gttgaaaaaa gtaaataaaa tgaatttgga ggttgaataa
      721 tgataaaaaa tgcatttgct tatgtaacta gaaaaagttt aaaatcatta attatcatat
      781 tagttatttt atctatgtca actttaagtc tcattagttt atctattaaa gatgctacgg
      841 acagagcttc aaaagaaaca tttgctaata taacaaatag tttttctatg gagataaata
      901 gacaagtaaa tccaggaaca cctagaggtg ggggaaatgt aaaaggtgaa gatattaaaa
      961 agatatctca aacagatagt atagactcct atgtaaaaag aataaacagt gttgcagatt
     1021 tagttgatta tgatattatt gaaactcaag agactcttgc gaatcaatca cctgaaagag
     1081 caaagaattt taaaagaaca gttatgttaa ctggggttaa cgactcaaca aaagaaacaa
     1141 aatttgtatc agaagcatat aaattagtag aaggaaagca tttagaaaat gaagataaaa
     1201 ataaaatctt aatgcataaa gatttagctg aaaaaaataa ccttaaagtt ggagataaga
     1261 taaaaataaa atctaattta tttgatgctg ataatgagaa aggtgcagat gaaacagtag
     1321 aagtagaaat taaaggtcta ttcgatgggc ataatagcgg tgtagtaagt gcagcacaag
     1381 aactatacga aaatacatta atcactgata ttcatacagc tgctaaagtt tatggtaata
     1441 ccgaagatac agctgtatac caagatgcaa cattctttgt aaaaggtgat aagaatcttg
     1501 atagtgtaat aaaagatctt gggaaattag atataaattg gagagaatat aatttgatta
     1561 aaagttcatc aaattaccct gcattacaac aatctatatc aggtatttac tcaatttcaa
     1621 ataaattatt ttttggctca ttaatatttg caggagttgt agtttcatta ttattattct
     1681 tatggatgaa tgctagaaag aaagaaatag cagtattact atcattaggt atatcaaaat
     1741 tagaaatttt tggtcaattc ctaattgaga tggtatttat atcaatcccc gcattcgttg
     1801 gttcctattt cttagctcaa tatacagctg acaagcttgg gaataatata ttgaataagg
     1861 ttactggtga tatagctaaa caaatagcta aacaatctgc atctagccaa ttaggtggtg
     1921 gggcagaagc tgaaggattt aacaagacat tatcaagttt agatatcaat gtattaccta
     1981 aattcatgat ttatgtagtt atatttatga gtttagtact tcttgtatca ttgattattt
     2041 cttcattcaa tatattaaga aggaatccaa aagaattatt aattgacaat aattaaaatt
     2101 ttcggtgctt tttagcacca aaaatgagag tgtaatataa accgtgtaag taaccatcaa
     2161 tcctaggcgg gtggttgctt cacggttttt atttttctaa aataagatca agaggtgatt
     2221 acacgatggc tagaaagaaa agaaatcctg atgctgagaa gttagctgaa tccattttga
     2281 atacttatca acccgaatct gtagaggata tgcaagatgt tttaaaagat gtgtttggtc
     2341 ccttatttga aaaaatgctt caaggagaat tgaataacca tttagggtat gacgcccatt
     2401 ctaaagagcc aaagaaacat gacaatcgac gaaacggtta tggcaataag acgcttaaaa
     2461 cgagctttgg taaagtaaac attgatgttc ccagagaccg ggaggcttct tttgaacctg
     2521 agttgattcc taatagaaaa cgagatgtct ccgacatc
//
LOCUS       DAZKNO010000225         2518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000225 DAZKNO010000000
VERSION     DAZKNO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.231"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..1160)
                     /gene="recA"
                     /locus_tag="ACG8MY_002570"
     CDS             complement(111..1160)
                     /gene="recA"
                     /locus_tag="ACG8MY_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0431718.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1269..2510)
                     /locus_tag="ACG8MY_002571"
     CDS             complement(1269..2510)
                     /locus_tag="ACG8MY_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0431719.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT          654 a          581 c          452 g          831 t
ORIGIN      
        1 gtacccactc gtataattaa ataactcacg atttctttat tcaataaaaa aacggcaggt
       61 caaggagagt acctactctt cagaccagcc gtttttgttt atcataattt ttattcttct
      121 actaaaggca attcttcttg tcctttttca tcttctggga cagcgacttc ttcaccaata
      181 ccataagctg ctctaactag agcagaaact tctgccatca tttcaggatg attagccata
      241 tagattttag cgttttcccg tccttgacca atccgatctt ctttataaga ataccaagca
      301 ccgcttttat caacaatgtc tttttctact gccatgtcta gaagttctcc ttcttgtgag
      361 atccctaaac catacatgac atctacttca gcgattttga aaggaggtgc tactttgttt
      421 tttacgactt tgatctttgt acggttacca acaatatctg taccttgttt caattgttct
      481 gcacgacgta cttccaaacg gatcgttgcg tagaatttca atgcgcgtcc tccaggagtg
      541 atttctggat tcccaaacat cacgcctact ttttcacgga tttggttgat gaaaatagcg
      601 attgtttttg ttttgttgat cgaaccagag agtttacgca atgcttgaga catcaaacgt
      661 gcttgtaacc cgacatgtga gtcacccatt tcgccgtcga tttctgctcg tggaactaaa
      721 gcagcaactg aatcgactac tacgatatct acagccccac ttgatactaa agcatcagcg
      781 atctctagac cttgttctcc tgtgtcaggc tgtgaaagaa gtagttcatc gatattcaca
      841 cctaattttt gtgcatattg cggatctaac gcatgctcag catcaatgaa agcggccgtt
      901 ccgccatttt tttgtacttc tgcaatagcg tgtagtgcaa ctgttgtttt acctgaactt
      961 tcaggtccat atacttcgat gatacgtcca cgggggtaac cgccgacacc taaagcgaca
     1021 tctaacgcaa gagagccact tggaattgta gagatttgtt ggtcgatttt ttcgcccaat
     1081 ttcataatag agcctttacc atagctcttt tcaatttttt tcagtgcagc atctaaggct
     1141 gctttacgat catctgccaa tacgataatc ctccttatat ttacaaatca gagcaacatt
     1201 tcattatcct tatcatatct tttttagtga aaaaaagcaa gaaaaaaccg aacaaatatt
     1261 cgcatttatc attttcttaa aagctgtttt ctcaaaagat caaatccttg cataaccgca
     1321 ttatatctga tagcagcacg atctctggca aagtgatatt tttccgaaac cacgccgtct
     1381 ttagatgaaa tagcgatcca tactgttcct gcagattgac cttctaacgt gtccggtcca
     1441 gccactccgg tgaaagctac actgtaatct gttcttgcta tctcacgtgc ttgaatggcc
     1501 atctgctcca cacattcttt gctgactgtt ccatattctg ctaatagctc cggatcaatc
     1561 tccagaaaac cagcttttgt ttggagcgag taggtaacga atccgcctgg aaatacttca
     1621 gatacaccag caatgtcgcc taatgcggct tggaaggcgc cggctgtcaa actttccgct
     1681 gctgttacag tctgcttgtt ttctttcagt agttccacta cgacttttgc tagcgaatta
     1741 tcatcgccgt atccataaaa atattcccct actcgttctt ggattttttc ttctaatgct
     1801 aaaagagctt gattccctgc ctgaacatca tttgttttca ctgtcagtcg taatgtcact
     1861 tcatttggtt tggcatatgg tgcgatcgtt ggattgatct gtgtctcaat cagatctttt
     1921 agctcagtga ccagtcgcga ttcaccaatc ccataaaagc gcaaaacttt agaaattagt
     1981 ttttcttcag aaggaaaacg ttcctctagt aaaggacgta cttgttccac aaacattggt
     2041 ttcaactcat taggcggacc gggaagaagg atatacgcat gtgtatctgt ttgataaaaa
     2101 gtccctaaag caagccctgt acgatttggt agaggaaccc cgccttcgat aatctgggac
     2161 tgttgcagat tgtttttggt catttttcga tgggttgttt caaaatatgc caataatttt
     2221 ttgtatcctt ccgtattttg tatgagagac ttaccgaggt gagccgcagt tgcttctttt
     2281 gtcaggtcat cttctgtcgg tcctaatccg ccacataaga gtatgagttc acttcttgtt
     2341 tcagctagag caatcaactc ttctaaacgc tgctgattat ctcctacaac tgtttgataa
     2401 taaacttcga tacctagatc agctagctgt tctgacaaga aagtggcatt cgtattcacg
     2461 acttgtccta gtaaaagttc tgttccgaca gctattattt ctgctttcat tacttccc
//
LOCUS       DAZKNO010000226         2478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000226 DAZKNO010000000
VERSION     DAZKNO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.232"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..159)
                     /locus_tag="ACG8MY_002572"
     CDS             complement(<1..159)
                     /locus_tag="ACG8MY_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0431720.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEEL"
     gene            821..907
                     /locus_tag="ACG8MY_002573"
     CDS             821..907
                     /locus_tag="ACG8MY_002573"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0431721.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1209..1433)
                     /locus_tag="ACG8MY_002574"
     CDS             complement(1209..1433)
                     /locus_tag="ACG8MY_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431722.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1799..2083
                     /locus_tag="ACG8MY_002575"
     CDS             1799..2083
                     /locus_tag="ACG8MY_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431723.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            2086..>2478
                     /locus_tag="ACG8MY_002576"
     CDS             2086..>2478
                     /locus_tag="ACG8MY_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431724.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFD"
BASE COUNT          833 a          475 c          385 g          785 t
ORIGIN      
        1 taattcctcg aaagaaaatc taactttttt caaaccacga tctttcatac gagcgcataa
       61 tgcaaaaaat aaatccatct gcttagcatt gaaatttttc agcggtacta aattcaattc
      121 attttgatat ttgactgtta attccgtttt ttcatccatt cctttcttcc ccccttacaa
      181 ataaataata acctttaaca accaactcgt aaataggttg gttgttatta tacaaaagga
      241 tattagtact cagataaaaa gttgttagta ctcagataaa aagttgttag tactcagata
      301 aaaagttgtt agtactcaga taaaaagttg ttagtaccct tctgtatccg ttgggagagt
      361 aaggctcaac tgccccctaa aaagggttct aaaaaatctt taaaaaataa ataaaataaa
      421 cgcgcgcgcg cgagcaaaat ttcccgccaa aaaatcaccc actttttaaa ataaacaggt
      481 tcgcgcatga tattaaactg ccaatctagc tccctatggt cgctgtaaga aggagtgatc
      541 gctacgctct cacattttac ttgcagcagt cgcctttagg ctccttcgcc aaaaataatt
      601 cattagggac tcctttgtgg cgtcctgaga gctcgtctaa gccgttctaa gacaagattc
      661 gataaaaact atgtccttcg ttctaaagcg ccttacaggc ggtcagggca agccctaaca
      721 accccaaggc aataaaaaac ttaggcttat ttttgctttt ttattgtata attgattgtc
      781 gcctatccga cagtcttgcg actggaagga ggtgtgtttc gtgttcacta actttatttg
      841 ctcccttctt gttgggcttt taagcgcttt gttcagttac tggttgaata accgaaacaa
      901 gaagtaaaac ttaattaagg cgactcagcc cacaaaaaaa gccccagaga atccgacctc
      961 tctggggctt tcgtgtgttt cgtgttcact tatttgcaat caaattataa cagaaataaa
     1021 tatttatttc aaaaatatcc tacaaaagtc taaaatccgt aattatttct tatatgcaac
     1081 atttacaaat aaagtcgctc tgagagctcg tatttttcga tttaagacgt tttatttagt
     1141 ttttggtgta tttcattggt ccaagctaaa aaactcttag aagcgatttt agagccattt
     1201 aaacccactt aattatattc tccatgaaat tcgctccatg cgtcaaaagc tgcgtctgaa
     1261 attccgccgt cttcgttcac tagttgcatt aatttttcca acgatcgttt tctttcgggt
     1321 gtcatatttt tatattccaa caatttttcg tctgttaaat cgaacttgat tgcttgaatt
     1381 gcttgctcta atttttctaa gttttgaaaa tattcgtaag gaaatttttc cattttttct
     1441 ttctccagtc tcacaatttt tctagaaata aaatcacttc tttgccgtct atactctcaa
     1501 aaaaaatgtc gtttgaaaaa ctaaataaat ttttgatcgt gcatttttaa atttggttat
     1561 cgattacacg tgcagcactc ccccccgaat tttaaagagc ataaggaaac gtgtacccac
     1621 tttctaaaaa aagtgggtag ataggctgtg cctatcacgt ttctcagaaa aaaatttgat
     1681 atcacttttt cgccatcgtg ggtagcttat gtatagcatt ttggcaaaaa acagctatac
     1741 aaatatagta tttgtatggt atacataaat tatacataat tgcaaaagga gattttttat
     1801 ggacatttta gttcggaata ttgatccaaa gtttgttgca acaatagaca aacgttgcca
     1861 agaactcacg aataaaacag gagaaaaatg gagtcgaaat aaatatctaa aagttttaat
     1921 cgaaagtgat tttgaccgtc aacttacgac ttacaaaaaa gaccaattcg atttattgct
     1981 cgataaattt attgctatcc aaacagaaaa tactcaaata ttaagcgagt atgtctacac
     2041 aaataatcaa atcattgaaa tgcttatcgg aggaatgata taaccatgag aaaaacttct
     2101 gtccaaaaac atgttacttt gtaccctagt catgttgacg ttttagattc catcatcgaa
     2161 aaaaatccag atatcaataa ctactcggaa gccattcgct ttttatgtat gagttatgaa
     2221 cccgatacca acacgaccac taccagcaat caggcaaaac taaatgctat gggaaaagaa
     2281 atttccatca tgactgaaat catcgcagaa tttgctgatt tatatctaaa agatacaggc
     2341 gttcttattg gcacaaattc taaagtatat caagaagtga aagaaaaaat cgaagctaaa
     2401 ctaaatccat caaatcgaaa tcgaccaaag aatcatcaaa aagaaaccag tcaaacagtc
     2461 acttctagaa atttcgac
//
LOCUS       DAZKNO010000227         2468 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000227 DAZKNO010000000
VERSION     DAZKNO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.233"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..764)
                     /locus_tag="ACG8MY_002577"
     CDS             complement(<1..764)
                     /locus_tag="ACG8MY_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0431725.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAVVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQR"
     gene            complement(1163..2464)
                     /locus_tag="ACG8MY_002578"
     CDS             complement(1163..2464)
                     /locus_tag="ACG8MY_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0431726.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
BASE COUNT          788 a          441 c          456 g          783 t
ORIGIN      
        1 catctttgaa ttttagagcc cgggaaaatg tcctgtatta cattttccat cccagacaat
       61 ccgtctgtac aaaataaaga aatacgttca acccctcttg atttaaggtc tttcagaagt
      121 tcttcccata tctcagcgga ctcgtttggt gcaatcatat agccaagaat ttcttttgta
      181 ccttctactg tgattcctag tgctatatgg accatttccc tggatacagt atcacggcgt
      241 aaaggcatgg atgttccatc aagatataca acggcatact ctttgttcaa tattctgttt
      301 ttgaaggaat ctacacattc cagagctttg tccgtaatat tagatatggt ctgtttagaa
      361 tagcgagctc cacataattt ttggatcatg gaacaaattt ctgaatggct cattccttct
      421 tcaaataaat ccaaaatcgt tgtttcggta aatagatctc gtcttctgta tttaggcaga
      481 agagaagtga aaaattctcc cattctatct cttggaatat tgagtgtaat agttccatat
      541 tttgtatcaa actttctttg ataggaacca tttctagaat tttcgttatt ggttctagcg
      601 tatggttcat aatcaagaaa atccgtcaac tcatatgcga gtatttcatt gagagatttc
      661 tccaactctg aacgaaacag ttcggttaca gcctgttcaa aagctggaag attgttgtca
      721 ttagaaaata ggtttttcat taaagatgta gtaaaatagt tcatgggtat attcctttcg
      781 taaattgttt ttggtcgaac taattttact aaggatatac ctttttttct acatttctag
      841 aaaaacgaat ttacacaaga tattttatgc tatccattta tttttgaaac tccatttgtt
      901 ttactacgtg tattaccttt atttttcttt ctatttttag gtgtctatta tctcttacgt
      961 tcgcttataa atgatgactc aaaatttaaa gcaaaataaa aataaaggaa gtagcctttt
     1021 gaacaagggc gcttccttta tttgttgtgg gacaccagag tatacttttt tataaaaaat
     1081 aggttgatgg gaaaccctaa gatttcccat caacactaaa tagtatagaa ccatattttc
     1141 gttacaaggt taagaatgac gactatagtt tttcatctac taattttgtt ttattgctct
     1201 ccatgacttt aaaaaatgga tagtagatga agatgctgat gacgatcaac agtaggcaaa
     1261 agagaatatt gcgccagtcc atacttgaaa gataagcttg tgcaaaaaaa ggtgtgaagg
     1321 aaggatcgac gataaatcca atcccaatcc atcctagttg ctgagcaaag taggtcagca
     1381 ataatgaaat gatgggcgta atcaaaaatg gaattgccaa aatagggtta aaaacgatag
     1441 gtaatccgaa aatcactggt tcgttgatgg aacagattcc aggaaccaat gacaattttc
     1501 caattgtgcg atattcgggt actttgcttc gcatcatcaa taaaaccaaa cccaaagtac
     1561 caccggctcc accaagtaca gaaatacgga acatttgtaa gttcattaag tgtgtcatgg
     1621 tttcaccatt tgccagttgt tcagcattca aaccagtttg agccattccc aatgtaaaga
     1681 cgatagggaa aataattgac gagccattga taccaaatag ccataggatg ttacccaaag
     1741 tcacgatcaa taagaaacca cctagactgc cagcgatgtt cgttgctgga gtcaggactt
     1801 ccatcaccgc ttcagggaag attttgtttg ttgctgccaa gaaaatcaga ttgatgccat
     1861 aaaagatgaa tatatttgtc agcagtggaa cgagcatatt gataaaggtt gctaccatgg
     1921 gtggaactgt atctgggagt ttcaacttaa tattttttac ttcaaatagt cggttgattt
     1981 ccactactag caagccgatg ataattgcga cgaacaatcc gtttgtccca aggaaattaa
     2041 gattgatggc accttcttcc actggtgtac aaaccattag ataaatgaca gatgcaacca
     2101 tgccattcat ggaaggatta attttgtatt cattggcaag ggaaaatgca atgccaaaaa
     2161 cactaatgaa tccgatgatt cccattgtca aattataagg ggctgtgatg agatcataat
     2221 ttgctacggc aaaatctttc cagctggcca taaatttcat gaaaatatta gctgtatctg
     2281 gattgtattt atcaatattg atgggtggat ttgcaaaaat cagaaaaaaa gaaccgataa
     2341 caataaaggg taggccaaac atcataccgc tggatatagc ttttaggtgg cgctgattac
     2401 ctatcttggc tgcgatgggg ctaagtatgt cattcagttt catgataaac gcagaatttt
     2461 ccatataa
//
LOCUS       DAZKNO010000228         2436 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000228 DAZKNO010000000
VERSION     DAZKNO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.234"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            921..1844
                     /locus_tag="ACG8MY_002579"
     CDS             921..1844
                     /locus_tag="ACG8MY_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0431727.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1860..2399
                     /locus_tag="ACG8MY_002580"
     CDS             1860..2399
                     /locus_tag="ACG8MY_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0431728.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT          914 a          383 c          418 g          721 t
ORIGIN      
        1 cccttttagt gagttttgta gaaagtacct ttaaaggtac tttcacatta cgaacgatac
       61 gtccgtagga cgtactgaaa gggtgggact tatgagcaaa ttaatgcaaa atttggctag
      121 caaaaaagct aaaaaatata tgctaataat cgagcgaaat aaacactttt tcaagtgagc
      181 gaatccgcca tgacaaagct gaagcagttt atttcagctt tgtcatggcg tgtgccgact
      241 tttttaaaag tctaggcaca cgagcaaata tctttatcaa gctctaattt gcgttttaag
      301 acgtttgaaa cgcctaagta tataaatata taagaatagg tttaagagtg cttaaatcga
      361 attttagagg gcatttagac aattttaggg ttgttagggc ttgccctgac cgcctgtaag
      421 gcgttttaag aacaagataa gggatttata ctaggactgt cttaaaatgt cttaaacgaa
      481 cgctcagagc ttctcaaagg gttccttatg aacggctttt agcaaaggag ccggaggcga
      541 ctgcgcagta taatgtgaga gcagagcgat cacacctttt tagcgaaccg tagggagcaa
      601 ggtttggaat tggcagtttt gataaattcg cgaacctgtt tagagaaaaa aggagtgatt
      661 ttttaggcgg gaaaattagc ctcccctctt tttatatctc tttttatatc tctttttata
      721 tctcttttat gtcggctgtg tccttactct cccaagcaat tcagagtttt ttcaagtagg
      781 ttttctacgc ttttcaagta ggttttctac gcttttcaag taggttttct acgcttttca
      841 agtaggtttt ccgacattat tgagtatttt tataaagtat gttactttta tttcatactt
      901 tataaaagta ggagaagctt atggcaaatg aaattgtaaa atatgaaaat agattaaatc
      961 atattcctct gagaaaattt aatagtcgtg aaatgaactt atttttttca attgcttcac
     1021 gtgtacgtga aaaaggtgta gatgagatta ttttttcatt tcacgatttg aaaaaactaa
     1081 gcggttatac agaacgtggg gaacaattta taaaagatct tagtagcacg tatgacaaat
     1141 tattaagtat taatgcttgg aatgatgatg gaaatacttt aactaaattt gttgctttca
     1201 caaaatatag cattatacga gataaacaac ttgttataat ttcagttaat ccagaattta
     1261 aaggactttt taatcaatta aagaactgga caagatttaa tctagagcaa tttactaatt
     1321 tggatagcac atattcaaaa actatgtttc gattattaaa acaatatcga actcaaggct
     1381 ggactgaatt ttccaaagaa aaattttttg atttactaga tattcctaaa agttattgga
     1441 atagtccgtc aaatattgac aaatttatct taaggccaat taaagaagaa ctcactccct
     1501 tatttaaagg attaactatt cgtaaaaaat atggtaaagg tcgaggtaaa cctgttatcg
     1561 gttatcgttt tacttggaaa gctgaaatta accatgctga cgatttttcc aaagggaaac
     1621 aagaagattt acgtataaaa ttatttaata ttgatcacaa tggagaatta acacgagaag
     1681 aaaaatggcg cgctaaagat cgaatactaa atcttccctt aggcactcat gaagctgatt
     1741 ttaaacaaca acaacaaact gaagaaaaac aggcagaaaa acgagaactt tcaaacaaac
     1801 tcaaacaaga tttactagga aatttacaaa acttattcga ttaagcttaa ggagtattta
     1861 tggcagataa aacaatcaaa gaacttgcgg aagaattagg agttagtaaa acagcaatta
     1921 acaaaaaagt ttctgacaaa aatcgaaaac tttggtttgc aaaaaacgga aacaagtttg
     1981 tgattaacga aaccggtcaa aaagctataa aatcaatgtt tttagtcgat aacgcaaact
     2041 taaatcgaaa actagttggc gaaaaatcaa aaaccaaaaa ccataaaaac accaaccaag
     2101 tttccgaatt aaaactcctt tctttcctag aaaaacagtt aaatcgaaaa gatatacagc
     2161 tacaagaaaa agataatcaa ataaaacaaa tgcaaaaatt actcgaccag caacaacaac
     2221 tcactctaca agcgaaccaa caaattgaga acctacaaga acagctccat ttaacttata
     2281 cagaagaaac tccaacaacc gaatcaactg ccttttctga aaaaaaagca gacaatacac
     2341 aaccacttat tgaaaaaaag tggtggcaaa tttggaaatc taatagaacc aatctctaaa
     2401 attttctaga agttagttcg gatagtagag gaaaaa
//
LOCUS       DAZKNO010000229         2410 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000229 DAZKNO010000000
VERSION     DAZKNO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.235"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..676
                     /locus_tag="ACG8MY_002581"
                     /pseudo
     CDS             61..676
                     /locus_tag="ACG8MY_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(816..1856)
                     /locus_tag="ACG8MY_002582"
     CDS             complement(816..1856)
                     /locus_tag="ACG8MY_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0431729.1"
                     /translation="MASIVKRGKSYRAQVSLYKNGQHKKLSKTFATKKEATLWALEME
                     LAKGEGKELAERTTTFADFFENWIYLVKVNDIKETTFQNYVRTLGVIRNLFEDIQLKD
                     LNDIVVQKKIDEYAETHSRKTTHEVLLKIKTALRDAYARGYIANDFARLVKTRGVNPP
                     KRNKALSITNFKKLRNYVLQHPEDEFNVLVLLALETGMRRGELLAIKPENLYEYGIEV
                     RHSISPTSDDTSLKTQNAKRDVSINKEVYELIRQIPVKENGYIFSFGGFKQSEQLAEL
                     LESLGIEKTTFHGLRDTHASFLFAQDIDIAYVSKRLGHINIQTTQNYYLELMPEKKHQ
                     QDADALNLLSSL"
     gene            complement(1951..2148)
                     /locus_tag="ACG8MY_002583"
     CDS             complement(1951..2148)
                     /locus_tag="ACG8MY_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431730.1"
                     /translation="MSINKNTKDYPYLLTREQASRFLGIDPKSFDKYIRPHNDLERFM
                     VGKHERYTMKSLIDFIESQSV"
     gene            complement(2192..2395)
                     /locus_tag="ACG8MY_002584"
     CDS             complement(2192..2395)
                     /locus_tag="ACG8MY_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431731.1"
                     /translation="MSNKKAMENINHIIEMIDFFQSGNGVINKKDNDSDYLRHIYTPE
                     DILGNLQHSAKEALKQLKNGELK"
BASE COUNT          770 a          399 c          430 g          811 t
ORIGIN      
        1 agagttgggc aacagaattt aaaaatgatc cggatttcag aattttaaag gagttttatt
       61 atggcgaaaa ttgggtatgc tcgagtaagc agcttagatc aaaatttaga ccgacaatta
      121 gaattattat cagagtgtga taaaattttt agtgataaag caagtgggaa agataccaat
      181 cgagaaggat ttcaagaaat gatgaaattc atacgtgaag gtgatattgt cgttgtgaca
      241 gaattaaaac gacttggacg aaataataaa gagctaacag aaacgatgaa tttaattcag
      301 ctgaagaaag ctacactgga agttttgaat ttgccagcgt taacaggtat cactgatgat
      361 aatttacgtc gattacttaa taacttaatt atcgaactat acaagtacca ggccgaagaa
      421 gaacgtcggt atattcgcga agcaacaaca aggaattgca ctcgctaaag caaaagggaa
      481 atacaaggga ggtacgctaa aatatcgtga gaatgatccg cgacttcaat tagcatttaa
      541 tttgtttttg actggatcta cagataaaga agttgaagag ctaacaggga ttaatcgtag
      601 aacgtttaga agatatcgaa tgaaacatgg tattactaat gaagttaggg agcaaaaaaa
      661 taaatctttc agttgaggct aatataattt tgcaagaaac caggtcttaa gaaagctcgt
      721 aaagcttcac aattctcaaa acgttaatag gtttgcctat ataccaacgt ttacaaaggt
      781 tttttggttc aagttggttc aaatcagctt gaacctcata aagaacttaa cagatttaaa
      841 gcatcggcat cttgctggtg ctttttttct ggcattaatt ctaggtagta attttgggtt
      901 gtttggatgt taatatgccc taaccttttc gatacataag caatatcaat atcttgtgca
      961 aacaagaatg atgcatgtgt gtcacgtaaa ccatggaagg ttgttttttc aatacctaaa
     1021 ctctccaata gctccgctaa ctgttcggac tgtttaaatc ctccaaaact aaaaatatac
     1081 ccattttctt ttaccggaat ttgacgaatt aactcatata cttctttgtt aattgacaca
     1141 tcacgctttg cgttttgtgt ttttaaagac gtatcatcag aagtaggact gattgaatgc
     1201 ctaacttcga taccgtactc gtaaaggttc tctggtttaa ttgcaagtag ttctccacgt
     1261 cgcatacctg tttcaagtgc aagtaataca agtacattaa attcatcttc tggatgctgt
     1321 aagacgtaat tacgaagctt cttgaagttt gtaatggata atgccttgtt gcgttttggc
     1381 ggattgacac cacgagtttt tacaagtcgt gcaaagtcat ttgcaatata tccccgagca
     1441 tatgcatcac gtaaagcggt tttaattttt aacaatactt catgtgttgt tttacgagaa
     1501 tgagtttccg catattcgtc aattttcttt tgaacaacaa tatcgtttaa atcttttaac
     1561 tgaatatctt cgaacaagtt gcgaatgact cccaatgttc gaacgtaatt ctgaaaagtc
     1621 gtttctttta tatcattcac ttttacaaga tagatccagt tttcaaagaa gtcagcaaat
     1681 gtcgttgttc gttctgcaag ttctttcccc tcaccttttg ctaattccat ttctaaagcc
     1741 cataaagtag cttctttttt ggttgcaaat gttttagaaa gctttttatg ctgtccgttc
     1801 ttatagagtg acacttgggc tctatatgac ttcccgcgtt ttacaatact agccatacta
     1861 tcaattcctt tcaaaaaata agttgataga tgctagttga atacacctgt attgtattct
     1921 gtaactagca taatatcaac tgttaaaaca ttagacagat tgtgattcga taaagtcaat
     1981 taaagatttc attgtatatc gttcgtgttt tcctaccata aaacgttcaa gatcattatg
     2041 tggacggata tatttgtcga atgactttgg gtctatgcca agaaaacgag atgcttgttc
     2101 acgagttaaa aggtaaggat agtctttagt gtttttattg atgctcatgt agcaccatct
     2161 cctttgtaat aaaataatat ccaagtaata atcacttcag ttctccattt tttaactgtt
     2221 ttaatgcctc tttagcagag tgttggagat tacctagaat gtcttcaggt gtatagatgt
     2281 gacgcaagta gtcagaatca ttatcttttt tattaatcac accattacca ctttgaaaaa
     2341 aatctatcat ttcaataatg tgattaatat tttccattgc ttttttattg ctcatatcgt
     2401 aatttcactc
//
LOCUS       DAZKNO010000230         2408 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000230 DAZKNO010000000
VERSION     DAZKNO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.236"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..2272
                     /locus_tag="ACG8MY_002585"
     CDS             119..2272
                     /locus_tag="ACG8MY_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0431732.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTNNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
BASE COUNT          821 a          424 c          531 g          632 t
ORIGIN      
        1 aagttgattt tatcttgaaa ttaggtaaag aatttcttgc tccgcccatt ttttcttttt
       61 tattaagaag tttttgggta caatgaagca tgaatgtaaa taggcggaag gataggaaat
      121 gatgaaagat tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa taagaaaagg
      181 tgcacaagaa cctaagaaca aaacgtttcg ccggtcccat gttccttttc gcttaaattt
      241 tctttttttc attatattta cgttatttgt tgcgttgatt gctagattgg gttatttgca
      301 gatcgtgaac ggcgaagaga tggaggcgcg agtgaagtct acttctacga ttacggtcca
      361 agaaagtact cctcgcggaa tgatctacga ctccacagga aaagctttag ttacgaataa
      421 agcgaatcag gccatcacat atacgagagg aacacaagtt acggctaaag atttgcttga
      481 aatcgcaacg aagctgaact cgctgatcga tgttccggta gatgaaaatc tgacagatag
      541 agataaaaaa gatttttggc tggctgatcc caaacatttg aaagaagcca caaaaagact
      601 ttcggcaaaa gaaaagcaat tagggaacag cgaacaatat agtgcgatgg tcaataaagt
      661 cacagctgat gagattaatt taaacgagga gcaattaaaa attgctacca tctttaaacg
      721 gatgaatagt gcatatgaat taaacactgt ctatatcaaa aactctggtg ttactgatca
      781 agagttagct gttgtggcag aacgcgcttc tgaacttcca ggagtttcga ctggcacaga
      841 ctggacacga gaatataatg cagcaagttc actgaaaagt attcttggca gtgtgacgac
      901 tgaaaagcaa ggacttccag cagatgaagc agagaaatac ctagcaaaag gttattctcg
      961 gaatgaccgt gtaggacaaa gttatttgga aaaacaatat gaagatgtcc tgcaaggaac
     1021 caaaacccaa tatgaagttt cccttgataa tgaaggaaat gtttcgaatc aaaaagaaat
     1081 cttctctggg gaaaaaggtt ctaatttaat gttatctatg aatgcagaat tccaatcaaa
     1141 agtagaagaa attttgaaaa ggaattatca aacattgatc aacaacggca aagcacagta
     1201 ttcgcctggt gcttatgcag tggctatgaa tcctcagaca ggagaagtgc tagcaatgac
     1261 tggtttcagt catgaacaag gttccaaaga aatcaccgag aatgctttag gcacgattac
     1321 tagcgccttt gctccagggt ctgtagtcaa ggctggtact ttgacagcag gctgggcaag
     1381 taacgcgatc agcggaaatc aagttttgat tgatgagccg attcgtctac aaggtgcttc
     1441 agaaaaaagc tctgtcttta atcgaagcgg acaagttgct cttgatgcag tcaaagcatt
     1501 ggaattgtct tctaatacgt atatgatcaa agtggcgtta aagatgttag gactagacta
     1561 tacacctgga atgggactac ctagtttaga tgaagaagct aaagcttatc agcaattgag
     1621 agactctttc aaagagtttg gtcttggtac aacgactggg atcgatttgc cgaatgaatc
     1681 tcctgggatc tcacgttctg ttgattatat gaagaagttc aatgcggata atggcaaaga
     1741 atggtatact ccaggtaact ttaccgattt ggcatttgga cagtttgaca catatacacc
     1801 aatccaatta gctcaatatg cttcaactgt cgctaatggc gggaaacgag tacagccgca
     1861 cctagtcaaa gcgatctatg gcaatgacga aaatggaaac ttgggtgaag tgaagaaaga
     1921 aattggcaca acagtagaaa atacggtcaa tatttctgca gaaaatatgg cgatcctccg
     1981 tgaaggattc caccaagttg tccatggtac cgatccttac acaacagcca aaccattagc
     2041 cagtgcgaaa atggaccttt ccgcaaaaac agggactgca gaaacggttg cggaaggtca
     2101 tccagatatt acgacagtca atagtaatat cgtggcatac ggaccaacaa acaatccgga
     2161 aattgccatc agtgttgtct tgccgaactt attagacgaa caagaccaca tgaacttgac
     2221 gattgctaaa gaaataatgg acgcgtatta tgatatgttc atggcaaaat aaaaaagagg
     2281 tcgtgaaaaa gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat agcttctcac
     2341 atttttgctt aaatttgact tcataccgag aaactggctt ttgaacaccg tttactcggc
     2401 aaaaaagg
//
LOCUS       DAZKNO010000231         2390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000231 DAZKNO010000000
VERSION     DAZKNO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.237"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..406)
                     /locus_tag="ACG8MY_002586"
                     /pseudo
     CDS             complement(104..406)
                     /locus_tag="ACG8MY_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            499..848
                     /locus_tag="ACG8MY_002587"
                     /pseudo
     CDS             499..848
                     /locus_tag="ACG8MY_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1120..1410)
                     /locus_tag="ACG8MY_002588"
     CDS             complement(1120..1410)
                     /locus_tag="ACG8MY_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431733.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1618..2310)
                     /locus_tag="ACG8MY_002589"
     CDS             complement(1618..2310)
                     /locus_tag="ACG8MY_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0431734.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          678 a          451 c          348 g          913 t
ORIGIN      
        1 aagtcctctt ttctgtgaat tggttgtcgt taactttatt ctacagaagg gacttccttt
       61 tttgtatgga ttttttcatt tacacaaaat attttatact ctcaacatac ttgcaaagta
      121 atactacaat tcctaacata atcgtagaat ttaataattt ccatagcata aagtgtttag
      181 aaaagaacat taagaatagt tctataataa ttcttgaact ccatgtctta taatgttcta
      241 tcgaagcacc caaatagcca agttcatcta atctacttgc ataaactacg tcatccgctc
      301 tcatagtcgg catatacata ttaaagagta aaaagatacc ccaaatccct attagtaaca
      361 agatcagttc tttgttattc gttatttttc tattatgttt gttcattttt atactccttc
      421 ttatatttcc taatagagta taacatagct ttaagcatta cttaaagata acttaaaagt
      481 tatcttttaa atacttaatt gttaaatacc ttatttgatg caaaagaaat ctacgatagt
      541 caacaaaaaa attttcatta tatccagaaa aatttttctt agttaaggac ctatggattg
      601 cagtaatgca gaattcgtcg aataaactgc caaagacata caatcatatt gcttttaaaa
      661 ttgacgaaca agatatagat tcgtttgtct caaaaatcca aatgttaggt ttaactgtcg
      721 aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat ttacttttac gactatgata
      781 atcacctctt cgagttacat accggtactc tccaggaacg tttaaaatcc tacaattcca
      841 ctacctgaga ctagtaatga ctagtctctt tttatggcct ctgatccccc cattgagcga
      901 atactaatag ggcttcgaat tgatctgttc aatctttaag caatatccct caaatgagtt
      961 tggaaagttt agtgattcat cacaaatact taaatacatg tagttatata tagattgagg
     1021 ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg tctgcatcca tctttccaaa
     1081 acaagcggct aggacatttt tgtctcagcc gcttgttttc tagtttattc tttcatcttg
     1141 tcggaccttt tttcttgggt atagactttt ctcatattct accacaattt tgggtttccc
     1201 taacacatga taggatctca caacaaattg ctttctatta ttataataac ctgtaacgac
     1261 taacttcatc ccttcgtcta cttcagctag gaagttcaaa ctatgggaag caatcaaaca
     1321 attgacccca tttaaactga aacggatcaa cggtctggca ctaacttgca aagtacggat
     1381 cttatctact gttccatatt ttttctccat aaccttcacc tcaataccat tatacgaaca
     1441 tacattccct ttgtaaagaa gaaactctgt tttatctttt tgactttttg tctattacct
     1501 ttcttaaaat catattggca cttcctgcga agatgaagga catgtactaa aaaaaagagg
     1561 cagcagtata gattactgtt gcttctttac taatttaggt ttacttgtta agaaagatta
     1621 tttacgcaaa tgttcttcaa caatttgctg agatttatca gctgtatctt ccacttcatc
     1681 ttttgctttt tctaactttt gaatatattg tttcaagctt gagttttcag cagttgcttc
     1741 gtctaatttc ttttgtgtat ctgttaactg agtttctaca tttgtttttt cttgtgtcag
     1801 tgttgcgatt tgttgattca aaccatttac agtattttgt aattctgaaa gttttgcttg
     1861 tgcttcttgt agtcttgctg tccaatctgc attcagatga ttgatttcat catttttttg
     1921 gttgatctca tctagtttga cattgatttc attttgtttg ctttgtagtt gtgcatcctt
     1981 gctgactaat tgactttcta agttttgttt ttctgctgcc gattgatttt tttggttttc
     2041 taattcattt acttttgttt ggtattcttg gacatctgcc tttgaatctt gcaatgtctg
     2101 ttctttttgt gctagtcgag cggtaagatc tgtaatcgct tgctctttat ctgtcaatgt
     2161 atgatcaagc tgtgtcagat tctcattgat tgtatcaata tctgtctctc caccccacaa
     2221 aataatttca tttgcgtacc gcgtgcctgt gtagccaatt agacatagaa atgcaacgac
     2281 aatcccacca gtaataaatt tctttttcat ttgcatagct ccttaatcca atacttttac
     2341 atgctaaaag taagtgatct caaacacaat gtcaagcgtt tttaaattac
//
LOCUS       DAZKNO010000232         2382 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000232 DAZKNO010000000
VERSION     DAZKNO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.238"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            216..1829
                     /locus_tag="ACG8MY_002590"
     CDS             216..1829
                     /locus_tag="ACG8MY_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0431735.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          750 a          423 c          453 g          756 t
ORIGIN      
        1 atccatcatt tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat
       61 ctatccttaa aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt
      121 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
      181 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
      241 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
      301 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
      361 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
      421 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
      481 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
      541 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
      601 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
      661 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
      721 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
      781 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
      841 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
      901 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
      961 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
     1021 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
     1081 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
     1141 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
     1201 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
     1261 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
     1321 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
     1381 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
     1441 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
     1501 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
     1561 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
     1621 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
     1681 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
     1741 acattacttt cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg
     1801 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
     1861 ggagttattt ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg
     1921 tatatagaat agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt
     1981 tatagtttaa gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata
     2041 aaaaaacatg ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact
     2101 acgcctaata ttacgaggca tcatattaat ctatcagcac tagtacctaa actagcatcc
     2161 ctttcgaata ttttttctta tataataaag tttgctttca ctcctctaat ctcaccaaat
     2221 cgactggctt gcgttgtgtg atactaggaa ctgattggtt tagaacgact tttctattgt
     2281 aatcatattt ttcgccatcc tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg
     2341 tgtattaaat aatttaattt ttttgttcct atttatttaa ca
//
LOCUS       DAZKNO010000233         2370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000233 DAZKNO010000000
VERSION     DAZKNO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.239"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            237..1346
                     /gene="pdhA"
                     /locus_tag="ACG8MY_002591"
     CDS             237..1346
                     /gene="pdhA"
                     /locus_tag="ACG8MY_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0431736.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1349..2326
                     /locus_tag="ACG8MY_002592"
     CDS             1349..2326
                     /locus_tag="ACG8MY_002592"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0431737.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT          774 a          452 c          506 g          638 t
ORIGIN      
        1 aaatgcagat gaaatataaa acgctttcct tattgtgatt ttattcacta atgctgttaa
       61 tatgcggatt ggcataattt cctttcatcg tgaaaatttg tgcttttagc ttgtatttct
      121 cataaatcca tgataattta ttaatgaaga cggttaaata gtgatacagt tttattcact
      181 gtttcaatcg cagaatgaag agattttctt atagatgaga gaggaatgtg tgacaaatgg
      241 caaacaaaaa agcaccgatc gacttccaag cactgctaga tgaagtcaat gcagatttcc
      301 cagtatatca agcacttgat caagatggaa acgtagttaa caaagattta gtacctgatt
      361 tatccgatga tgaactcgtt gaattaatga ctcatatggt atggtctcgt gtattagacc
      421 aacgttcaac agcgctgaac cgccaaggac gcctaggctt ttttgcacca actgctggac
      481 aagaggcaag tcagctggct agtgcttacg catttgataa agaagatgtt cttcttccag
      541 gttatcggga tgtacctcaa ctagtaaaac atggtttacc tttatctcaa gcttttttat
      601 ggtctcgagg acatgctgca ggtaattctt atccggaaga attgaaagct ctaccaccac
      661 aaattatcat cggtgcacaa tatgtacaag cagccggtgt tgcattaggt ttgaagaaac
      721 gaaacaagaa aaatgtcgtc ttcacttata ctggtgatgg cggttcatca caaggtgatt
      781 tttacgaagc aatcaatttc gctggcgctt accatgcaaa tgccgtattt tatatccaaa
      841 acaacgggta tgcgatctct acacctcgtg aaaaacaaac tgcagcaaaa acacttgcac
      901 aaaaagctgt cgcagctggg attcctggta ttcaagtcga tggaatggat ccgcttgcag
      961 tatatactgt atctaaaatg gcacgggaat gggcgatcag tggaaatgga ccagtcctaa
     1021 tcgaaacact gacttatcgt tatggaccac atacactatc tggtgacgat ccaacccgct
     1081 accgtacaaa agacacagat gacgattggc agaaaaaaga tccattgatc cgcttccgta
     1141 aatatttgac ggaaaaagga ctttggtctg aagaaaaaga agaacaagtc atcgaagcaa
     1201 ctaaagaaga aatcaaagca gctattgctg aagccgacaa agtaccaaaa caaaaggttt
     1261 cagatttctt gaaaaacatg ttcgaagtat ctccacaatc aatcaaagaa caaattgcaa
     1321 tttatgaagc gaaggagtcg aaataatcat ggcacaaaaa acaatgatcc aagcaatcac
     1381 agatgcctta gcacttgaac tagaaaatga cgagaatgtc gttgtttttg gtgaagatgt
     1441 tgggaaaaac ggaggagttt tccgggcaac tgaaggattg caggaaaagt tcggagaaga
     1501 ccgagtattc gatactcctt tagctgaatc tggaatcgca ggattgagtt tcggtcttgc
     1561 gttagaagga tttcgtccag ttcctgaaat ccaattcttt ggttttattt tcgaagcaat
     1621 ggacgaagta gtagctcaaa tggctcgtac aagatatcga atgagcggca ctagaaatct
     1681 acccatcaca attcgttcgc catttggtgg cggtgttcat acaccagaat tgcattcgga
     1741 taatttagaa ggtttgatcg ctcagtctcc tggtattcga gtagtgattc cttccaaccc
     1801 ttatgatgca aaaggactat tgatttcttc tattagaagt aacgatccgg ttgttttcct
     1861 agaacacatg aaactatatc gctcattccg agaagaagtg ccggatgaag cttacgaagt
     1921 tcctttggat aaagcagcag ttactcgtga gggaacagat gtatctatta tcacatacgg
     1981 agcaatggtg cgtgaagcaa tcaaagcagc agacaatctt gcaaaagaaa atatatctgt
     2041 tgaaatcatt gatcttcgta cagttgcgcc attagatgta gaaacgatca ttcaatccgt
     2101 ggaaaaaact ggtcgcgtag ttgttgtcca agaagcacaa cgacaagcgg gcgttgccgc
     2161 gcaagtagtt tctgagatct cagaacgcgc tatcctttca ttagaagccc caatcggacg
     2221 agtttctgca cctgatacag tcttcccatt tggacaagca gaaaatgtct ggttgccaaa
     2281 tgcaaaagat atcgaagaca aagtaaaaga aatcgcggaa ttttaagaaa agaagggacg
     2341 aaacaaagtc atggcttatc aatttaaatt
//
LOCUS       DAZKNO010000234         2343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000234 DAZKNO010000000
VERSION     DAZKNO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.240"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..2010
                     /locus_tag="ACG8MY_002593"
     CDS             148..2010
                     /locus_tag="ACG8MY_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0431738.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2010..2342
                     /locus_tag="ACG8MY_002594"
     CDS             2010..2342
                     /locus_tag="ACG8MY_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0431739.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          842 a          347 c          458 g          696 t
ORIGIN      
        1 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
       61 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      121 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      181 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      241 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      301 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      361 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      421 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      481 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      541 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      601 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      661 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      721 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      781 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      841 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      901 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
      961 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1021 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1081 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1141 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1201 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1261 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1321 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1381 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1441 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1501 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1561 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1621 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1681 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1741 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1801 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1861 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1921 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     1981 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2041 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2101 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2161 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2221 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2281 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataatt
     2341 aat
//
LOCUS       DAZKNO010000235         2311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000235 DAZKNO010000000
VERSION     DAZKNO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.241"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..804
                     /locus_tag="ACG8MY_002595"
                     /pseudo
     CDS             1..804
                     /locus_tag="ACG8MY_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(911..1942)
                     /locus_tag="ACG8MY_002596"
     CDS             complement(911..1942)
                     /locus_tag="ACG8MY_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431740.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(1949..>2311)
                     /locus_tag="ACG8MY_002597"
     CDS             complement(1949..>2311)
                     /locus_tag="ACG8MY_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0431741.1"
                     /translation="LVDAAETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYM
                     LTNLLIGGRFQTVRIYMLRRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLS
                     LKQTREEKQSKKKPLKEL"
BASE COUNT          702 a          547 c          410 g          652 t
ORIGIN      
        1 aaattggttg cctcgctgtt cattggtaga tgaatttcgt tcattgaaaa atcaagtcgg
       61 tccctattct ttttcttgta tggatggcga tactgggaaa ttactggata ttcttccttc
      121 acgtaagaag aaagatctca tttcttattt tatgcaattt gaacgacgtg cacgtttaaa
      181 cgtaaaaata ctcgtaacag atatgaacgc ctcttatgct ttgctgataa aagagtgctt
      241 tcctcatgcc aaactcgttg tggatcgctt tcacatcgtc aagcacctca ttcgcagttt
      301 taaggatatt tgactatgag tcatgaaaag cttcgaccga aacgatccaa tacaagccaa
      361 acactatcga caagtgaagg ctttatctcg acttctgatc aaacgacagg acacgcttgt
      421 ttatgataaa tggacaaaat ggcgtaattt tggttgggcc tatttaaccg aaagtgaagt
      481 cgtagaacgt ctactttcta cttctgatga actacgaata gcttatgctt actatcaaga
      541 aattcttcaa gctttttatg acaaagaagc ggacatcttc tttcagcttg taaaaacaat
      601 gcccaagtcg gttccaagag aactgcatca tataaaaaaa gcgtttataa actatgaatc
      661 cggcattcgt ttagccttag agttgcctta ctcaaatggc aaaatagaaa atctgcatac
      721 ccacatcaaa gcattgaaac gaatagctta cgggttagga tgagaaccag aattttctta
      781 atcaatcatc tgatcactta ttaaacataa aaaaacatct aaagcagaaa atcaattcta
      841 ccttagatgt tcttatatca ctctatcagt cctatttgac aaagagccag aaatgtttgc
      901 cgactataca ctagctttgt tgtgcaaaat gctgagtatc aatttccaca gggattgcta
      961 gaatatcttt cagctgaatg tataaggcat cgctaatttt ctcttcccga tcgttcaacc
     1021 gatccactaa aatttctttg ccctccagcg tttgaaaaac ataccgttga atgttgccta
     1081 aaataaatga ttccttccat agaaccggaa tcttaacccc ctgaggaacc ggttcaaatt
     1141 gaatcagctc cggtcttaaa taataaatgc agcgattgcc cttagcggaa aactcgtggg
     1201 tatactgatt cagctcatac cattcgaaac gattatagtt gccgataaat tccgcaacaa
     1261 atcggctctg aggattctga tacaattcct tcgaggtgga aacctgagcc acccgcccag
     1321 cttccatcac aaaaatccga tcactgattc gcatcgcctc ttcttggtca tgagtcacaa
     1381 aaatcatcgt catccctagc gattgctgga tagcgcgcaa atcccgctgc agctgttttc
     1441 taattcgggc atccaaagca cttaatggct catccagcag caggactttc ggttcagtga
     1501 ccaatgagcg agccagtgcc acccgttgtc gttgaccgcc agaaagaaac tggacatttt
     1561 tatctgcctg ctccgtcata tccaccaatg atagcatctc aaaaaccttt tcctgaatca
     1621 cctcaggact cagcttcttt accttcaaac caaaggctac attttcaaaa acagtcatag
     1681 ttggaaacaa tgcatactgt tgaaaaacaa agccgatatt gcgatccttc gtaggcttat
     1741 cggcaatatt ctcattgtcc agcacaatct caccgctctt tggttgttcc aatcccgtga
     1801 tacagcgcag caacgtagac tttccgcacc cgctgggacc tagtaaagta accagctcac
     1861 ccttttcaat ctgaatttca tcaatagcta aaatctgttt ttcatcaaaa aacagattga
     1921 gatcatgtat ttctaaaaac attttccgtc acaactcctt taagggcttc tttttcgact
     1981 gcttctcttc cctcgtctgc ttaagggaca gctgttgctt ttgtcgataa gtcagcataa
     2041 aggtagttaa agcaattccc accataaaca gcaaatacaa aaccatcaca gcgctagcca
     2101 aatggccgtt ttcgttcatc cgccgcagca tatagatgcg taccgtctga aatcgaccgc
     2161 caatcaacag gttcgtcagc atgtactccc caaaaaccga agaaaaaatc atcagagaaa
     2221 ctagggtcac accgatacgg atgtttggaa acagcacctg gataataatc gtagacatcg
     2281 gggcgcccaa ggtttctgct gcatccacta a
//
LOCUS       DAZKNO010000236         2307 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000236 DAZKNO010000000
VERSION     DAZKNO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.242"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            314..1738
                     /gene="istA"
                     /locus_tag="ACG8MY_002598"
     CDS             314..1738
                     /gene="istA"
                     /locus_tag="ACG8MY_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0431742.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPKKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVTQTKTKTEQAVLTD
                     GFLRFSMHYGFETQVCNPRSGNEKGSMENKVGYVRYNFFSSTPIMKDFASFNEALAVE
                     LSHDRERIHYEKHVIINDLWNTEKTDLLALPDTDYPVFKEIEVKVNKYNEIKLDNERI
                     HVPRVRNYSIIYGLLRFDSYQLISTDGEIISEGLRPYMMKKRAIDWQAILPDWRRKSR
                     AMHYSRYWKYLPERIKLFLSHPDWKEQEKRLNQISGLLVTHDMLGIDTHFYDMFSAST
                     TTDAYDIDWHQYDAFLSLTGEVID"
     gene            1738..>2307
                     /locus_tag="ACG8MY_002599"
     CDS             1738..>2307
                     /locus_tag="ACG8MY_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0431743.1"
                     /translation="MDQAKSLETACKLLRLSHVLPTLVRKDLDYSFETKEDWLMYLLE
                     KELACREELKTARLLKQAKFPNKRTLEDYEWHDHICLPNATTKDELLSLSFIHQKENA
                     VFVGTPGTGKTHLACGLGQLAAKSGIDTRFWRVSDLVNELEHHWKNNTLEVFKHRFDK
                     VKLIILDEMGYVPFTKEGAELLFQLISDWY"
BASE COUNT          729 a          452 c          500 g          626 t
ORIGIN      
        1 aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc ataccgaacg
       61 gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt aaatcgagtc
      121 atctatttcg tttattcact ttttcaagtg gacattcatt aagctgttta tggcagaata
      181 aaagtacata caagtgcaac gaaaaactac acaactccgc aaatcgatta agttaagata
      241 aaaagccatt gcgggcaacc tgaaattcct ctaaagttat agtctcgaca ctgtaactgg
      301 aggtaagaaa ggtatgctca caatgaccga tattaatact atcagaaatt tacgaaataa
      361 tcatgataaa tccattaatc atattgcaaa ggagctagat gtaaattggc gcacggctaa
      421 aaaatatgca gatgatgatt tgctgccgca acctaaagca cgcaaaaaga ctggtatgat
      481 gtatacagaa aagtggggag gcatcgtttc cctatggctt tcagaagatg ctaagctgcc
      541 aaagaaaaag cgtaggactg ctgaagcaat gactactgct ttaaaagccg atggctttcc
      601 aggatcctac cgaacagtct gtgtattcat tcaagattgg aaagcgacac actacttgga
      661 aacactggaa gaccaaggct ttgagcgtct tgaacacccg ccagcagaag cgcaacttga
      721 ttttggtacg atggaagttt gtcacgatgg agcgtttaaa gatgtgaagg tattagtgat
      781 gactttccct tttagtaatg caggattcgc cgttgtactc cccgccgaga atcaagaatg
      841 tttgttggaa ggcatgaaag aactcttcag ccaatcaggt ggcgttcctc ggaaaatacg
      901 gattgataat atgtcaacgg ccgttactca gacaaaaaca aagactgaac aagcggtgct
      961 gactgacggt ttcctccgat tttctatgca ctatggtttt gagacacagg tgtgcaatcc
     1021 acgtagcgga aacgagaaag gtagtatgga aaacaaagtc ggctatgtac ggtacaactt
     1081 cttttcatct acaccgatta tgaaggattt cgccagtttc aacgaggcat tagcggtgga
     1141 gctgtcacac gatcgagagc gaatccatta cgaaaaacat gtcattatca atgatctatg
     1201 gaacacagaa aaaacagatt tgctagcttt gccggacacc gattacccag tatttaaaga
     1261 aatcgaagtc aaagtcaata agtacaacga aattaaatta gataacgaac ggatccacgt
     1321 tccacgtgta cgcaattatt caattattta tgggctgctt cgttttgatt cctatcagct
     1381 aatcagcact gatggtgaga ttatttcaga aggacttcgt ccttatatga tgaaaaaacg
     1441 agcaatcgat tggcaagcaa ttcttcctga ttggcgcaga aaatcgcgtg ccatgcatta
     1501 ctctcgctat tggaagtatt tgcccgaacg aatcaagtta tttctcagtc atccagattg
     1561 gaaagaacag gagaagcgtt taaatcaaat ctcaggttta ttggtgacac atgatatgtt
     1621 agggattgat acacattttt acgacatgtt tagtgcctcg actactaccg atgcgtatga
     1681 tattgattgg caccaatacg atgctttctt gtctctaaca ggggaggtca ttgactagtg
     1741 gatcaagcaa aaagtttaga aactgcctgt aagttgctga gactttccca tgttcttccc
     1801 actttggttc gcaaagactt agattacagc tttgaaacca aagaagattg gttgatgtat
     1861 cttctggaaa aggagttggc ttgccgtgaa gaattgaaaa ctgcccgact cctaaaacaa
     1921 gcaaagttcc caaataaacg taccttagag gattacgaat ggcatgatca tatatgtttg
     1981 ccaaatgcga cgactaaaga tgaattattg agtctatcgt ttattcatca gaaagaaaat
     2041 gctgtatttg tgggtacacc cggtaccggt aaaacacatc tagcttgtgg attgggtcag
     2101 ctggcagcca aatcaggtat cgatacacgc ttttggcggg tatcagattt agtcaacgaa
     2161 ttggaacacc attggaaaaa caatacctta gaggtcttca agcatcgctt cgataaagta
     2221 aaattaatca ttcttgatga gatgggttat gtgcctttca ctaaagaagg cgcggaactg
     2281 cttttccaat tgatcagcga ttggtat
//
LOCUS       DAZKNO010000237         2287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000237 DAZKNO010000000
VERSION     DAZKNO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.243"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2287
                     /locus_tag="ACG8MY_002600"
     CDS             <1..>2287
                     /locus_tag="ACG8MY_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFQ0431744.1"
                     /translation="IFSGDSPFKEILDKIPPEKNKRLEKPREVSITDPHLNDEGEVEI
                     PKEVVLGKSKELFGEKIYSSIEVVDEVVSSFEEAEEKQKESIKKLAKDITASFSDGFN
                     NLKESFKLNKGQTEKLKESVNNSIEDIITEKSLTLSNQVKEIEEEYTKKKQEAEKKND
                     ETELKAAIQQYEEEKQKIEESFADDINKEVENTINTAVEEQIEKVEEKKKKTTEDDVR
                     DHLRGFARTIPAFLMAYGDNQTRLENFEENIDEATFEDITSITIEEFKKLRDGFEYED
                     DNGNRKVIHGLFNEVVFNASIREFLDTKKRLANYFDESLEEDIFDYIPPQKTNQIFTP
                     KRVVKKMVDKLEEENPGIFSDRTKRFADLYAKSGLYITEIVKKLNEGLKGQIPNQQER
                     IKWILENQVYACAPSNIICNIVKNFVYSHTINVSTKNLIEVDTMKLASEEKLSTEIYE
                     LFGDDRLKFDVIIGNPPYQDETIGENKTYAPPIYHKFLEESYKLADKVEMIHPARFLF
                     DAGSTPKAWNRKMLADEKLKVIDYVDDSSKVFPNTDIKGGIAITYRDAQKRIGPIGTF
                     TKYPELNTILRKVKMDNFNSIDKIIYSADSYKFTQALHDDYPDAEARLSKGHAFDVRS
                     NVFDSIGHIFKNEKPSDNSEYIRIIGRQNHKRIEKWVKRDYIGEHPNLDKYSVLLPGS
                     NGNGDFGETFASPIIKEAAVAHTQTFISIGHFDNIIEAQNLLKYVKTKFARAMLGILK
                     ATQANSRPKWKWVPMQNFTNES"
BASE COUNT          929 a          298 c          439 g          621 t
ORIGIN      
        1 atcttttcag gagattcacc atttaaagag attttagata aaataccacc tgagaaaaat
       61 aaaagattag aaaaacctcg tgaggtttct attactgatc cgcatttaaa tgatgaaggg
      121 gaagtagaga taccaaaaga agtagtgtta ggtaaatcaa aagaactttt cggagaaaaa
      181 atttattcat caattgaagt ggttgatgaa gttgtatcat ccttcgaaga agctgaagaa
      241 aaacaaaaag aatctattaa aaaacttgcg aaagatataa cagcatcatt ttcagatgga
      301 tttaataact taaaggaatc cttcaaactg aacaaaggac aaaccgaaaa gctaaaagag
      361 tcagtaaaca attcaattga ggatattata actgagaaaa gtttaacact aagcaatcaa
      421 gttaaagaga tagaggaaga atatacaaag aaaaagcaag aagcagaaaa gaaaaatgat
      481 gaaacagaat taaaagcagc gattcagcaa tatgaagaag aaaaacaaaa gatagaagag
      541 tcttttgccg atgatattaa taaagaagta gaaaacacaa tcaacactgc tgtcgaagaa
      601 caaatagaaa aagtagaaga aaagaaaaag aaaacaacag aagatgatgt tcgagatcac
      661 ttacgaggat ttgcacgtac aatacctgca ttcctaatgg catacggaga taatcagact
      721 agattagaaa actttgaaga gaatattgat gaagctacct ttgaagatat tacaagtatc
      781 acaattgaag agttcaagaa gcttcgcgat ggttttgaat atgaggacga taatggtaat
      841 agaaaagtca ttcatggttt attcaatgag gtagttttta atgccagtat acgagagttt
      901 ttagatacta aaaagcgttt agcgaattac tttgatgaaa gtttagaaga ggatattttt
      961 gactatattc ctccacagaa aacgaatcag atttttacgc ccaaaagagt agtaaaaaaa
     1021 atggtcgata aattagaaga agaaaatcct gggatttttt ctgataggac gaaaaggttc
     1081 gccgacttgt atgcgaaaag tggtttatat attacggaga tagtaaagaa attaaatgaa
     1141 ggtttgaaag gacaaattcc aaatcaacag gaacgtatta aatggatact tgaaaatcaa
     1201 gtatatgcgt gtgcacctag taatattatt tgtaacattg ttaaaaactt tgtctatagt
     1261 catacgataa atgtgtcaac caaaaatctt atagaagttg atacaatgaa gttagcaagt
     1321 gaagagaagt taagtactga aatctatgag ttatttggag atgatagatt gaaatttgat
     1381 gtaattattg gtaatcctcc ttaccaagat gaaacgatag gagaaaataa aacgtatgct
     1441 ccaccaattt accacaagtt tttggaggag tcttataaac tagctgataa agttgaaatg
     1501 attcatcctg ctcgattttt attcgatgct ggtagtacac caaaagcatg gaatagaaaa
     1561 atgttagccg atgaaaaatt gaaggttatt gactatgtgg atgatagctc aaaagtattt
     1621 cctaacaccg atattaaagg gggtatagcg ataacctacc gtgatgctca aaagagaata
     1681 ggtccgatcg ggacctttac aaagtatcca gaattaaata ctatccttcg taaagtgaag
     1741 atggataatt ttaattcaat tgataaaatt atttattcag cagatagcta taagtttaca
     1801 caagcattac acgacgatta tcctgatgca gaggcacgat taagcaaagg tcatgcattt
     1861 gatgttcgct ctaatgtatt tgatagcatt ggacatatat ttaaaaatga aaagccctca
     1921 gataatagtg aatatattag gattataggt agacaaaatc ataaaagaat agaaaaatgg
     1981 gtgaaacgtg attatattgg tgaacaccca aatttagata aatatagtgt tcttctacca
     2041 ggatcaaatg gaaatggtga ctttggagaa acatttgctt ctcctataat aaaagaagct
     2101 gctgttgcac acacacaaac gtttattagt attggtcatt ttgacaatat aatagaagct
     2161 caaaacctgt taaaatatgt aaaaacaaag tttgcgcgag caatgttagg aatattaaaa
     2221 gcaactcaag ctaatagtcg accaaaatgg aagtgggttc caatgcaaaa ctttactaat
     2281 gaatcag
//
LOCUS       DAZKNO010000238         2271 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000238 DAZKNO010000000
VERSION     DAZKNO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.244"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1014
                     /locus_tag="ACG8MY_002601"
     CDS             <1..1014
                     /locus_tag="ACG8MY_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431745.1"
                     /translation="YLDGYFTANGFNSLEYEYALENPEQFEEYSNSMLELYNNHHALL
                     CDYAGSSEAQGEFSKSRPYYIQQTIIANRNYISEFSNIQVNGKSLNEDIYDTPTILVS
                     RKYKSDETFIKEHITQEYFRLMNYNQFYGIPEKERHINEFNIVYVDDNSTIKVNTENG
                     FSDMSEPIIIIDTGNFAGLYYLDSLNTRNLFFQMDSREEFSSLLAEYGLKQLVTAGTL
                     LTPYLMQLENVTFVLKTLTMFAIVFIISLLFILYISNYVDIFVNRKKYATKEIMGFSH
                     FKTLKNRYIFWGIELIASCILTAINYNFACLFAIILIDYIFCELLYRIYILNALYEIE
                     KGA"
     gene            1017..1667
                     /locus_tag="ACG8MY_002602"
     CDS             1017..1667
                     /locus_tag="ACG8MY_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0431746.1"
                     /translation="MSLITLKNIKKVYGKNQILFENLSLEIEKGEFVGLVGKSGAGKT
                     TLLNIIGLITDISEGIITIDGQQKLSVNSRNAMLLRRYTIGYLFQNYGLIEDESVLWN
                     LKLALEYKKMKKEDKLKVINEYLKQFNLSDMLNKKIYQLSGGEQQRIAIIKLILQGSQ
                     IILADEPTSGLDKENEQVVMKLLKELNEKGITIIMVTHNMDLRNYFSRVINVETFR"
     gene            complement(2127..2271)
                     /locus_tag="ACG8MY_002603"
                     /pseudo
     CDS             complement(2127..2271)
                     /locus_tag="ACG8MY_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          860 a          295 c          367 g          749 t
ORIGIN      
        1 tatctggacg gatattttac ggctaatggt tttaattctt tagaatatga atatgcatta
       61 gaaaatccag aacaatttga agaatattct aattcgatgt tagaactgta taataatcat
      121 catgccttgt tatgtgatta tgctggtagc agtgaagcac aaggggaatt tagtaaatct
      181 cgtccatatt atatacagca aacaataatt gcaaatagaa actatattag tgagttttca
      241 aatatccaag ttaatggaaa atcattaaat gaggatatat atgatacgcc aacaatatta
      301 gtttcccgta aatataaaag tgatgaaact tttattaaag agcatataac tcaagagtat
      361 tttcgactga tgaactacaa tcaattttat gggatacctg agaaggaaag gcatatcaac
      421 gaatttaata ttgtgtatgt cgatgataat tctactataa aagttaatac agaaaatggc
      481 ttttcagata tgtcagaacc tattatcatt attgatacag gcaattttgc aggtctttac
      541 tatttggatt ctttaaatac aagaaattta tttttccaaa tggattcaag agaagaattt
      601 tcttctttat tagcagagta tggtttaaaa caattagtta cggcaggaac attacttaca
      661 ccatacttaa tgcaactgga aaatgtgacc tttgtcctta aaacactcac aatgtttgcg
      721 attgtcttta taatatcatt actgtttatc ttgtatattt caaattatgt agatattttt
      781 gtgaatcgaa aaaaatatgc aactaaagag attatgggat tctctcattt taaaacatta
      841 aaaaatcgtt atatattttg gggaatagag ttgattgcat catgtatatt gactgctata
      901 aattacaatt ttgcttgtct ttttgcaatt atacttattg actatatatt ctgtgaatta
      961 ctttatagaa tttatatctt aaacgctttg tatgaaatag aaaagggggc ataagtatgt
     1021 cattgattac attaaagaac ataaaaaaag tatatggtaa aaatcaaatt ttattcgaga
     1081 atttgtcgtt agaaatagaa aagggtgaat ttgttggtct tgtaggtaaa agtggtgcag
     1141 gtaagacaac tcttttaaat ataattggat tgattacaga catatcagag ggaataatta
     1201 ctattgacgg tcaacaaaaa ttatcagtaa acagtagaaa tgcaatgctt ttaagacgat
     1261 atacgatagg ctatctattt caaaactatg gacttataga agatgaaagc gtattatgga
     1321 atttaaaatt agctcttgaa tataaaaaaa tgaaaaagga agataaactg aaagtaatca
     1381 acgaatattt aaaacaattt aatttgagcg atatgctcaa taaaaaaata tatcagttaa
     1441 gcggtggaga gcaacagcgg attgcaatta tcaaattaat tttacaagga agtcaaatta
     1501 tactagccga tgaaccaact tctggacttg ataaagaaaa tgaacaggta gtgatgaaac
     1561 ttctgaaaga attgaatgaa aagggcatta ctataattat ggtaactcat aacatggatt
     1621 tgcgtaacta tttttccaga gttataaatg tagaaacttt tagatagttt attaaaggga
     1681 gatatacttt tttgtgcaat ctcccttgtt ctttttctgt catatcaaga ctcgttcaat
     1741 aatggtaaat tcgtggtaaa atagctactt ttttagttga tgaatagccc ttaaatactg
     1801 tatttatgcg gataatcgga ttttttatac caagtttgga tatatcaaca aatcatttga
     1861 taattatgat cttggtttta taattaattt aaaaaaaata aaaactatcg atgacttact
     1921 cacaaataat ttagttctca aaaaagcgta ctgtgataca ttctaatgac gagagcgtct
     1981 ttaccggaaa aatcaatctt ttagattcaa aaaaactagt actttctata atagattacg
     2041 aaagcatcac aaagacgaac aaaaaaaaca ataaatatag atgcagactg tagacatcat
     2101 ccataaaatg gactttgtct acagtctgaa acgagatctt tcttcttacg tgaaggaaga
     2161 atatccagta atttcccagt atcgccatcc atacaagaaa aagaataggg accgacttga
     2221 tttttcaatg aacgaaattc atccaccaat gaacagcgag gcaaccaatt c
//
LOCUS       DAZKNO010000239         2224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000239 DAZKNO010000000
VERSION     DAZKNO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.245"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..682
                     /locus_tag="ACG8MY_002604"
     CDS             <1..682
                     /locus_tag="ACG8MY_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431747.1"
                     /translation="TEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFIQRVEEQLYL
                     ESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEKYIMNVYLERSELVENFVSY
                     TICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDDYYLVFTRFLTCGTESIVLM
                     GMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKRMPYQ
                     RFERKKRQ"
     gene            complement(729..965)
                     /locus_tag="ACG8MY_002605"
     CDS             complement(729..965)
                     /locus_tag="ACG8MY_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431748.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
     gene            1063..1242
                     /locus_tag="ACG8MY_002606"
     CDS             1063..1242
                     /locus_tag="ACG8MY_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431749.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1373..1642
                     /locus_tag="ACG8MY_002607"
     CDS             1373..1642
                     /locus_tag="ACG8MY_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0431750.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1635..1892
                     /locus_tag="ACG8MY_002608"
     CDS             1635..1892
                     /locus_tag="ACG8MY_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0431751.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          818 a          310 c          448 g          648 t
ORIGIN      
        1 aacggaagat gtgtcacact ttttaacaaa caaagaacat ttgaatgaag taatcagacg
       61 gattcaaaaa gactttagtt gtatggaaga aatgaagtac tttattcaaa gagtcgaaga
      121 acaattgtat ttggaaagtg aaacgagtca aacgaaggtc acagtcagcc ataatcgttt
      181 agacgtgtat aaacgtcgtc ctgaaacggt aaaaaaagga acggagcttc tacgactaga
      241 aacagaaaaa tacatcatga atgtttacct agaaaggagt gagctggtcg aaaactttgt
      301 cagctacacg atttgcagga aacactatta ctcagaatca gaatattatt caatttttga
      361 gagcatggaa ttaatggaag aagaaatgga agagctaaac agcaaagatg cgacgataga
      421 cgattattac cttgtattta ctagattttt gacgtgtgga acggaatcta tcgtgttaat
      481 gggaatggat tatgaactcg ctgtacccga agcatttttg agggaatatg tagaagaagt
      541 agataaccca ggcgacgact ggtacacgac tttaactgat tttttagaca cctatactta
      601 cgacgataca gaagaaatcc aaaatgaact tgaccgtaaa cgaatgccat atcaacgatt
      661 tgaaaggaaa aaaaggcaat aatcatttat accgaaaagt gagaatgtgt gaaataagga
      721 caaaaaacct agtaactaag ggaatggttc ttatttcgca ccaatcgctc caaccgcccc
      781 cgccgcttag gtggtgcgaa ataagcgggc aacacccgat aacctaagag aaagaaggag
      841 atccatatga ataaaatcat tcctctattg ttagtcctat ttgttggcga aatacttatg
      901 cgccatgctg cagctgtgca tttaaacttc gtagtatttt tggcaatact ggtattttgt
      961 ttgatctttt ggcgctttgt attagccctt gtgagtctac tattcaagat gattgcagcc
     1021 atatttttca tagggttatt tcgatgaaag gagagagtac taatgccaat tgcaatagga
     1081 aataaacggt tacccgttac gttggatgaa aagagacaaa aagaattgca gcaactaaag
     1141 cagaagtacg gcaaaagtga atccaggatt atgtgtattg cgttagattt attgattgcc
     1201 caagaaaaag caggatttga ggtaccagca ctcaaaaagt gacgtcactt tttatcctaa
     1261 aaactaaaag tgatagcact tttaattata agaagttaga atattaatca tttgcttaat
     1321 tgtacaatat aatgtacaat tgttttatag aaataaataa ggggtgaaag gaatggaagc
     1381 agtagcttat tcaaatttcc gccaaaattt acgtagttat atgaaacaag ttaatgagga
     1441 tgctgaaaca cttattgtaa caagtaaaga tgtagaagat acagttgttg tattatcaaa
     1501 aagagattat gattctatgc aagaaacgtt gagaacactt tctaataatt acgtcatgga
     1561 aaaaattcgt cgaggagatg aacaattctc caaaggtgca tttaaaacac atgacttaat
     1621 cgaggttgaa tctgatgatt aaggcttggt ctgatgatgc ttgggatgat tatctttatt
     1681 ggcatgagca aggaaacaaa agcaatataa aaaagattaa caagttaata aaagatatcg
     1741 atcgttcccc ctttgctgga ttaggaaaac ctgagccatt aaagcatgat ttatctggaa
     1801 aatggtccag aagaattaca gatgaacata gactgatata tagagttgaa aatgaaacga
     1861 tatttattta ttctgcaaaa gatcactatt aaccaatcgg aagtaaggaa agggtcagaa
     1921 acttaaaagt ttttgatcct tattttattt accctagtca tttaaaaagc taatatagct
     1981 tagtgttgat tgttattaat gaatgtgttt gttacgcgta ttacggatat aaggttagta
     2041 aaatcatttc taaagttgag gaaaagtaaa tataaatggc ttaaatttca acaatttgaa
     2101 gttgaataga tatgttataa tactattgta gtgtgggatg ttagttacta aaggatgacg
     2161 cttatatata tgactgaata gaataagcaa taggtttaat aatctatttt aaattttttg
     2221 tact
//
LOCUS       DAZKNO010000240         2212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000240 DAZKNO010000000
VERSION     DAZKNO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.246"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..2196
                     /locus_tag="ACG8MY_002609"
     CDS             115..2196
                     /locus_tag="ACG8MY_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0431752.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT          726 a          348 c          509 g          629 t
ORIGIN      
        1 tccgatgcag atgtttttca ttttttcttt cggatacctt gacatatgaa taaccgttca
       61 tttataatta gttatgaaga gatattcata tgttgaggcg aggaggtcat taaaatgaaa
      121 agttacagat tagaaggttt agattgtgcg aactgtgcaa tgaaaatcga aaaaggagtt
      181 cagaagatca atggagtcaa ggaagcaacg gtaaacttca cttctggaaa actaacgatt
      241 gacgcagagg aagatcattt ggctacgatt gaacaagaaa taaaaaaagt agtgaaggaa
      301 ttagaaccag atgtcaaagt aacagaaata gataaggaaa aagtatctga acatggaaat
      361 gaaaaagaga gaaatacgct gttccggatt ttattttccc ttgcaggtat cgctttattg
      421 ctattatttg attttaatga accaattcgc ttgatagggt atttattgat ttatctgctg
      481 ataggatatg atgtagtgaa gaaggcagtg atgaatatcg tcaaaggaaa aatctttgat
      541 gagaattttc ttatgtctgt tgctacgatt ggcgctatgt tgatcggtga atatccagaa
      601 gccgtagcag tgatgctatt ttatcaaatc ggcgagtatt tccaaggatt ggcagtcagt
      661 cattcaagaa aatcgattcg cgaattgatg gcgatccgtc cagaaacagc tcacgtgcag
      721 acgacggaag gattgatgac cgtgaaccct gaagacgtgc agatcggtca gtttgtactt
      781 gtgaagcctg gtgaacgtgt accgttagat ggaacgatca tcgaaggaga atcattagtc
      841 gatacgtcag cacttacagg agaatcagtc cctaaatctg tatatgttgg tgaaacagta
      901 ttaagtggat ttatcaataa aaataaacca tttttagttc aagtagaaaa atcttatgaa
      961 aattctacta tcagcaaatt attagaatta gtagaaaatg ccagcagtaa aaaagcacca
     1021 gctgaaaatt tcattactaa attcgcgcgc tattacacac cagtcgttgt aggattggcc
     1081 gttttattag ctgttttacc tccacttgtt gtttctggag cggcgttttc tgaatggatc
     1141 tatcgagcat tgacattttt agtgatctca tgtccatgtg cattagtgat ctccgtaccc
     1201 cttagtttct ttggcggtat cggaggagca agtaaaatcg gtgtattagt aaaaggttca
     1261 aattacttgg agttgcttgc acaaactgag acagtggtat tcgataaaac cggcacatta
     1321 acgaaaggtg acttttcgat ccagacgatc gatacaaaaa tcgatccaaa gatattcatg
     1381 caatatgttg cgagtgcaga acagttttct acacatccga ttgctcaatc tgttttagaa
     1441 ggctacgcag gtccgctttt accaacagct aatatccaag aatttgcagg tgaagggatc
     1501 ttagctgaag ttgatggcaa acaagtatta gcaggtaatc acaaattgat ggaaagattt
     1561 gaaatctctt ttccttcaag tcaagaaatc ggtacattgc tttatctagc gatcgatcaa
     1621 agttacagtg gttatcttgt tattgcagat accttgaaag aagatgccgt agacgcattg
     1681 gttcaattaa aaaaggctgg tgtgaagaac acagtcatgt tgacgggcga ttcgaaaaaa
     1741 attgctgatc atatcgggaa gcaagtaggc gtggacaaga tatatagcga attgcttcca
     1801 gaagataaag tgcagcggtt agaggaaatc ttacaaagaa acaataaaaa gaccgcattt
     1861 gtcggagatg gaatcaacga tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg
     1921 ggtggattgg gaagcgatgc tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa
     1981 ccatcgaaga ttgcagaagc aatccattta gcaaagaaaa cattgaaaat cgtcaaacag
     2041 aacattgtct ttgctattgg gataaaaatt ttggtacttg ccttaggtgc ttttggtttt
     2101 gcatcaatgg gagatgcagt atttgcagat gttggggtga cagtattagc tgttttgaat
     2161 gccatgcgaa gcttgcatgt aaaaaataaa cgttgatcaa tgaagaggac ga
//
LOCUS       DAZKNO010000241         2197 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000241 DAZKNO010000000
VERSION     DAZKNO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.247"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..320)
                     /locus_tag="ACG8MY_002610"
     CDS             complement(<1..320)
                     /locus_tag="ACG8MY_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HFQ0431753.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFF"
     gene            446..856
                     /gene="tagD"
                     /locus_tag="ACG8MY_002611"
     CDS             446..856
                     /gene="tagD"
                     /locus_tag="ACG8MY_002611"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFQ0431754.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            1270..2070
                     /locus_tag="ACG8MY_002612"
     CDS             1270..2070
                     /locus_tag="ACG8MY_002612"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0431755.1"
                     /translation="MRLRKQHYHYQSCGKYFTAHTYLVVPSCFISKQVHYTILEELTE
                     RQALKTLTKRCDLSVTTFQRTLSALSAMLRQMLDYLPHCLLFDEFRSLSISHGKFSFS
                     CMNRETGKLFDILSSRRKKDLIAYFMRFTRKAHMGVRYIVTNMNAPYFSLVKESFPNA
                     QIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKDTSQAKYYRQLKPLYKLLLKSDDELDY
                     TTFKKWQNFQWRYLTESEVVDRLPSISYELKIAKQFIKSCLAIITRNS"
BASE COUNT          802 a          381 c          329 g          685 t
ORIGIN      
        1 aagaaaaaga ttaattgcca catgattaac ttgatatttt cgctatatcc atattttaaa
       61 ttcattaaac tgcttactag catcgcgcca ataaaagcaa ttaatagcca atcatatttt
      121 ttaagatttt tttctctaaa tgcaataatt gcatctataa tgatcaagac aactcccgct
      181 attccagcag gccaaaaaag taaagtatca tatctagtag gtaatagtaa atacgggcct
      241 attgctcgta acattagaat aaaaccaaaa taaataaaat accaaccttt cacatatttt
      301 aaaaattttt cattcaacat ttttcctcca tccacctatt tctcataaac atttcttatc
      361 ctttttagga taaactggtt ctttcaattc taacaaaaaa atgctaaact aacaatgtat
      421 attaatgaat ttcgagaggt gaaagatgaa aagagtaatt acttacggta cctttgattt
      481 gcttcactac gggcatatca atttactgaa acgagcaaaa caatatggag attatcttat
      541 tgttgccctt tctacagatg aattcaattg gaatgcgaaa caaaaaaaat gttattttag
      601 ttatgaaaaa agaaaacagt tacttgaagc tattcgctac gttgatttag ttattcctga
      661 agaaaattgg gaacaaaaag taaaagatat aaaagaatac catgcagatt atttcgtgat
      721 gggcgatgat tgggctggac aatttgatga acttgaacaa ctaacagatg cacaagtaat
      781 ttatttacca agaacacctg agatctctac aactcaaata aagaacgaac ttaattcttt
      841 aaatagtgat aaataatatt ttttaaacaa tgaaagaaca atatatctgg ataagacgta
      901 aaaaaatggc tctataattt ctaggactga tagaattttc tgtcgatcct agattttctt
      961 gtaaaaaaaa cagacataca aaaatttcga taaaatagag tcgtctaaaa catcaatctg
     1021 aaaggaagat tttgtatatc catttctcgt tctatcaaag agcacttcac tattaaagat
     1081 aaaaatattg aaattaattc ttttcaaaaa actttttata aggacaagaa agccatcgta
     1141 atcgaaggta cattagttcg cacttttaga cattgtctat tttgtaaaga aactgcgaag
     1201 cagctcgtga aaaacggcaa gaagatttca atgattatct taaatcgcag tgggaataaa
     1261 taaacctatt tgcgtcttag aaaacaacat taccactacc aatcatgcgg aaaatatttt
     1321 actgctcata cgtatcttgt cgttccttca tgttttattt cgaaacaagt tcattacacg
     1381 atattagaag agttgaccga gcgtcaagcg ttaaagacac ttacgaaacg ttgtgatctt
     1441 tcagtaacga catttcaacg gacattatct gctctatcgg caatgctgcg acagatgcta
     1501 gactatttac ctcattgttt actattcgac gagtttcgct ctctttcaat ttcccatgga
     1561 aagttctcct tttcttgtat gaatagagag acagggaagc tctttgacat tttgtccagc
     1621 cggagaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc tcatatgggg
     1681 gttcgataca ttgtcacaaa tatgaatgcc ccttattttt cattagtcaa agagtccttt
     1741 ccaaacgccc aaatcatcat tgattgcttt cacattggac aacacttgaa caagtttttc
     1801 gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aagacacttc acaagctaag
     1861 tactatcgac aattgaaacc tttgtataaa ttacttctaa aatcagatga cgagttggat
     1921 tacacaacat tcaagaaatg gcagaatttt caatggcgct atttaacaga aagtgaagta
     1981 gttgatcgtc taccctcaat ctcttatgaa cttaaaattg ctaagcagtt tatcaaaagc
     2041 tgcttagcaa ttatcacaag aaacagctag ctacttcgag aaacgaccaa agaagttccc
     2101 tcgaaacttc actatctcaa aaagcctttt tcatatctga aaaaggcttt taactagcat
     2161 tttcgaaaaa gtattctaat aacaaaatta gagaatc
//
LOCUS       DAZKNO010000242         2156 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000242 DAZKNO010000000
VERSION     DAZKNO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.248"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..>2156
                     /gene="acm"
                     /locus_tag="ACG8MY_002613"
     CDS             41..>2156
                     /gene="acm"
                     /locus_tag="ACG8MY_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0431756.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          847 a          343 c          420 g          546 t
ORIGIN      
        1 gtaggtttta tttttagtta aaatagggga gatagaaaaa atgaaaaagt gtagaaatat
       61 tattttatca atgttattta ttattactaa tataacatca ctgattcctg ttcatgtcta
      121 tgcggatgca ggcagagata tcagcagtaa tgtcacttcg ttgaccgtgg acccaacgaa
      181 tattacagat ggaggaaaca taaaagtaaa attctcgttt gacgaaaaaa aacaaaatat
      241 ccaaccaggt gattatcttt ggatcaattg gccaagtgaa ggaaatattc gcggtgaagg
      301 attccaaaaa gaaatcccat taatgattga aaataaaaat gttggaactt taacggtcag
      361 aaaagattct gcgcaggttg tgtttaatga aaatattaaa aacctagact cagtagaagg
      421 ttgggggcaa tttgagatac aggccagaaa cgtaaccgat accggtgaag aaaatacggg
      481 accattcacc gtgacgagcg gtgataaaac agctactgta aatgtgacaa agccagcttc
      541 tggttcttct tctagcgttt tttattataa aacaggagac atgctacctg aagatactaa
      601 gcacattcga tggtttttga atattaacaa taacggcact tatgttgaac aaccagttaa
      661 aatcagtgat gaaatccaaa gtggacaaag attagatcca agtacgtttg aaattaatca
      721 aatacactta ggtgaacaga aggtttatag aggagaagag ggaatccaac aatttttaca
      781 agatttcccc agcgctacat ttaatttttc agtaactgat aattacattg aaataactat
      841 accaaaaaac tttgtcaatc ttaggaaaat aatggtgagt tataagacga tcatagaaaa
      901 cccagaacaa ataaattttg agaatcattc ggaagcttgg tttaaagaat ttaataaacc
      961 tgctgttgat ggagaaagtt tcaatcatac agttaaaaat atttcagctt ctggtggagt
     1021 caacggtaca gttagaggcg aattgaagat tttcaaatat atcaatgaca cagagattgg
     1081 tatcccaaat gtgactttcg aattaagacg agcagatgaa cagcctattc aaggacagtc
     1141 tagtatcctg ttgacttcga atgaacaggg agaagctagt atcaaaggcc tgcaagtagg
     1201 agactatgtt gtaaaagaaa aagaagcacc caattggatt gattttgatc cactaagttc
     1261 aaatgaactc aaattctcca taaacgaaaa tgatacagag ggagtcagtt tacctattta
     1321 taacaagaaa aaggttacga acatcacagc aactaaaatt tggaacggtg gaaccactcc
     1381 aagaccgagt atttatttta aattattcag agcaagtaca aacaattggg aaccagttcc
     1441 tgatgcagaa acaaaacgat tagacaatgg aataacttca gtgacttggg aagatatcca
     1501 acagtatgat gatagtggaa acgaatatac gtttaaagtg caagaagtag atcaaaacgg
     1561 aaatgattat gtcccttcag gttatcaaaa aattgaaaac gggctagtcg ttacaaatga
     1621 gaataaagaa gtcatttcag tctcaggaca aaaaacatgg gaagacaatg agaaccaaga
     1681 cggcaaacga ccgacgacga tcacagtaaa tctattagcc gacggtcaac cgatccaaca
     1741 caaagaagta agtgaaaaag atgattggcg ctatcgtttc acaaatctgc caaaatacag
     1801 agacgggcaa gaaatcatct atactgtaac ggaagacaac gtgcccgaat acagtacgac
     1861 gattgacggg tataacatca aaaacagtta tacaccggaa aaaacaaata tatctatacc
     1921 aggtagccat ataacaccgt ggaaaaattt tccagagaag acagagagag agaaaattag
     1981 aaatctattc tctcaaaaat tgcaaatatt tgctcataca ggagaatcca gcgaacaaag
     2041 aagcaattat attagtaaga gagctgtacc taaacagata acaaataaat caaaaagcat
     2101 tcttcctaaa acaagtagta aaaaatctag ttttgctgta atcattggtt tattga
//
LOCUS       DAZKNO010000243         2143 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000243 DAZKNO010000000
VERSION     DAZKNO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.249"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..591)
                     /locus_tag="ACG8MY_002614"
     CDS             complement(97..591)
                     /locus_tag="ACG8MY_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431757.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(584..898)
                     /locus_tag="ACG8MY_002615"
     CDS             complement(584..898)
                     /locus_tag="ACG8MY_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431758.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1114..>2143)
                     /locus_tag="ACG8MY_002616"
     CDS             complement(1114..>2143)
                     /locus_tag="ACG8MY_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0431759.1"
                     /translation="INMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEYYENLE
                     KRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNIEVYQF
                     SKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGKRYSFE
                     SVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQKQFSDR
                     EGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTISIKKT
                     DQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIENQQKR
                     QQNFEMEF"
BASE COUNT          618 a          404 c          296 g          825 t
ORIGIN      
        1 aacggaacaa ctttattttc ctcagccatt ctcccagttc cattttcaaa aatcacaaaa
       61 ctgatattac cttaaaaact actaacaaaa taaaacttat aaagtaaatg gcaacccaac
      121 tatgtaacca ttctctctac gtactacgat ttggtagtcc tgcctttcaa tcggtaattc
      181 ataatagtaa tttactgttt cttctttgcc actttcaata tactctggat tatccaatat
      241 catatcatac tcataaagtt cttcaccatc cttattttca atcgaccatt gttgtggacg
      301 tatcggattt tctgtatcat ttataagtgt ataggtcact tgtaatacac gatcatgttg
      361 tgcaaattca tttctttttt cggtcacttc aatattttta aatgttagtt caaccccatc
      421 tgcctgataa gtttcaccta tttttaaatc ttttgttaca atttttattg gcttttcttc
      481 tggaaaatgt ctggctttaa ttgttagata acttccaaaa agaatcaatc ctagtagcac
      541 taaaagaatt cctttaacga gccactgaaa cgggaagtaa actttaaaca tcttttttct
      601 cctccgtttt gtatttgttt tttagagaat acgataactt ttgatcaatg tcatccagct
      661 tttcaattac ttcattactt atcattgatt ctagttctga aattttttgt tcaaatttaa
      721 tgctttcagg atcattaaat ttattatttg atggactagc taacactggt aaatttgaaa
      781 ccataacacg aaacatattt gcctttgaac caaaaattcc tttgttttgg ttgtaaaaat
      841 tgttgaatat ttctttagtt tcttctgaag ccgagacttt aaaatatgga tagttcattt
      901 ttttcttcca cctttctttt tttgggaggg agtctgtagg gagcttgctc cctcagagat
      961 ttacccgcca tctgacttgc cagaaagtag cctagcgtga gcggggcgga ttgactggca
     1021 agtagatggg tagctggcgt atgtcctaaa aactgcttct ttccaagcaa ctcttcatag
     1081 gcaaaaaact ggaaagtcca ctaacaagcc cattcagaat tccatttcaa aattttgttg
     1141 ccttttttgc tgattttcaa tcatttgttg tgtgaattca taatcctttt tagcacgata
     1201 catttttaca tcgtcattca ttatgcgact gatttgatag tgaatctgtt tagcagaagg
     1261 tgtatgagaa caattttcaa aaccctttgc cggtagaaat aactctttct gatcagtctt
     1321 tttaatagaa attgttttac ttttcgcact acgtaaagtt tgaggattca catccgtatg
     1381 aagtttaatc gtgtattctt gatcatcttc taaagacatt gtatttacaa aatgcttgtc
     1441 tacctctcgc aagaagtcat catatttaat gaatttttca tcgccttcac ggtcgctaaa
     1501 ttgcttttgt gcgatggaat ctaattccac aaacggtaca tttaactcac cagttttgta
     1561 atttactcca ctaattagcg acataacttg ctctctttcc atattaaaaa aattttttat
     1621 atgtgattct tctaaattct tttcaagttg ttcagaaaca gattcaaatg aatatctttt
     1681 ccctaacttt cttgcactaa aagtacgttc gacatactga cttttttctt ttctagattg
     1741 tttatctctt cctaaagtaa ttaactcacc tctacgctca tagaaattta caccttgttt
     1801 gtaaagaaca tagccaagtt tgttatttgt ttttgaaaat tgataaactt caatgttata
     1861 ttcttttgcc aaatgatccg aaaaatcttc aaaagaattc acttcgcatt tttccaacgt
     1921 tgcttcgatc gcattttgca caaactcttg ataattaatt tttccatatt tcatcttaga
     1981 aggatcgttt gaataaagat tggttctctt ttctaagttt tcatagtact cattattcac
     2041 tgattcaaga ccatgttcat tgaggacttg atcgttaatt tttcgcatta gttctaaatc
     2101 tttagggtca atacgcaacc ttttacatgt ttccatgtta ata
//
LOCUS       DAZKNO010000244         2142 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000244 DAZKNO010000000
VERSION     DAZKNO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.250"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1342
                     /locus_tag="ACG8MY_002617"
     rRNA            <1..1342
                     /locus_tag="ACG8MY_002617"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..1552
                     /gene="rrf"
                     /locus_tag="ACG8MY_002618"
     rRNA            1437..1552
                     /gene="rrf"
                     /locus_tag="ACG8MY_002618"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1566..1638
                     /locus_tag="ACG8MY_002619"
     tRNA            1566..1638
                     /locus_tag="ACG8MY_002619"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1599..1601,aa:Val,seq:tac)
     gene            1642..1714
                     /locus_tag="ACG8MY_002620"
     tRNA            1642..1714
                     /locus_tag="ACG8MY_002620"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1675..1677,aa:Lys,seq:ttt)
     gene            1722..1803
                     /locus_tag="ACG8MY_002621"
     tRNA            1722..1803
                     /locus_tag="ACG8MY_002621"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1756..1758,aa:Leu,seq:tag)
     gene            1821..1893
                     /locus_tag="ACG8MY_002622"
     tRNA            1821..1893
                     /locus_tag="ACG8MY_002622"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1854..1856,aa:Thr,seq:tgt)
     gene            1905..1976
                     /locus_tag="ACG8MY_002623"
     tRNA            1905..1976
                     /locus_tag="ACG8MY_002623"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1937..1939,aa:Gly,seq:gcc)
     gene            1993..2078
                     /locus_tag="ACG8MY_002624"
     tRNA            1993..2078
                     /locus_tag="ACG8MY_002624"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2027..2029,aa:Leu,seq:taa)
BASE COUNT          565 a          473 c          626 g          478 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg tgagagtagg acgtcgccac gcttaagtta
     1561 tccatggagg tttagctcag ctgggagagc atctgcctta caagcagagg gtcagcggtt
     1621 cgatcccgtt aacctccata tgagccgtta gctcagttgg tagagcatct gacttttaat
     1681 cagagggtca caggttcgag ccctgtacgg ctcatatttt tgcgggtgtg gcggaattgg
     1741 cagacgcact agatttagga tctagcgcct tacggcgtgg gggttcaagt cccttcaccc
     1801 gcataattta agttttacat gccggcttag ctcagttggt agagcatctg atttgtaatc
     1861 agagggtcga gggttcaagt cctttagccg gcatctttaa acacgcggaa atagctcagt
     1921 ggtagagcac caccttgcca aggtgggggt cgcgggttcg aaccccgttt tccgctttgc
     1981 caaagcaatc gcgccggggt ggcggaactg gcagacgcac aggacttaaa atcctgcggt
     2041 gagtgatcac cgtaccggtt cgattccggt cctcggcatt tcaaatatgc gcccatagct
     2101 caactggata gagtgtttga ctacggatca aaaggttagg gg
//
LOCUS       DAZKNO010000245         2127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000245 DAZKNO010000000
VERSION     DAZKNO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.251"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..733)
                     /locus_tag="ACG8MY_002625"
     CDS             complement(83..733)
                     /locus_tag="ACG8MY_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431760.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(833..1342)
                     /locus_tag="ACG8MY_002626"
     CDS             complement(833..1342)
                     /locus_tag="ACG8MY_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFQ0431761.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQVVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1705..2088)
                     /locus_tag="ACG8MY_002627"
     CDS             complement(1705..2088)
                     /locus_tag="ACG8MY_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0431762.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
BASE COUNT          616 a          427 c          282 g          802 t
ORIGIN      
        1 aatacactta tttaaaaact tctttcccag attttcttcc atttttacag ttataactag
       61 tattatcatg aaaaatagcc ttttattgtc tatgggaata ataatttaaa attggtactt
      121 ttttattaat tagttcatcg cccaataatc tctccagttt cttagcatct cctgactcaa
      181 ccggaattat ccaagcccag tccaaataat tttttaaaat ctcatcatat tttgcaaata
      241 tttcattgcc atattccgtc cttaaaatta tattttcagt gccagacaag tgatttgtat
      301 tgagttgata aaaaagatct ccatctaaaa aaatatcatt agaagaagat ttccccacag
      361 taatgaccat tccatcagct ctatgtgtta catacaaata attgtatgac ctcacttcac
      421 ctaatgaatc aattatttct tgccctttag ctctaatata ttgatctcta ttacctatct
      481 cactcttatt taacaaaagt tttttcatgt caaatttaat cggctgtttt atttttgtaa
      541 attcgcttct actactaatc caatccgaga ttaatttttc aacatgtccc agagtaaact
      601 ctgatttatt cgctaaatac tttttttgtt gtattaaaga catctttccc cgaagctcaa
      661 aattgagcct acatcgattc tctatctctt caaaaagcat cttatcttca tcattcttat
      721 attcatcatt cataaaacca atccagctcc tcgattaaga taatatttta atctcataca
      781 aaaactagct ctcatcaaaa caaccattgt tttgatttct catgaaattt ctttatactt
      841 ttcttcgatt ataaattcct aaccccttga gaaaattacg catatattta tctccgcaag
      901 gcttaaaatt tcgttggcct tcttttctca aaaatgcccc gatttcgcct tttgatggat
      961 agagttctgc ctcagcaaaa acttctaaaa tatcatcagt ggtcatagac aaggcgattt
     1021 ttaatttttt tagcaacaca ttgttgatat atttgatttc cttgcttttt tgcggcgtgt
     1081 ctactatcgg cttatctttt tgcggaccac gcgccaagag aatcaggcca tttaggaatg
     1141 cgtcaaaaac ttggttgttg atggtttttt catacacatt attttctgca ttatctttgt
     1201 tcttatcctg tactttggtc agtaccacct gagcttcttc tttagtcagt ggtacaccgc
     1261 ctagctcaaa agctttaatc aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca
     1321 ataacctatc attgttattc atttgttcct ccgaagtatt tagtctttac tttcttacgt
     1381 actttttgtc ttgctaagat ttttaggatc aatgataaaa accgcatcac ttatgatacg
     1441 gagctgggta tcatgagtga atgatattta taaatcactg gatattgaag tatattctgt
     1501 tttctcaact tatttagtca gattttttaa tgaagatttt ttgagtaaaa tttctataat
     1561 tcctacttat tgaaaagtag tttgtagtgc tgcttctacc tctctcatct gttctacaaa
     1621 ttccaaataa cttgacgcag cttgcggaat gaaaaaatga tatgtgaata tttctcctcc
     1681 tagcccgctt aatatgccta ttttttatac ccatgatttg caggaagggg atcggaagta
     1741 ggttgcagaa agccgccttt cataatctgt cggaaaccgg acatcaaaac ggattatttt
     1801 acttgtcaca tttgtatttt cttgtgagga agtgccaaat acattattta cttgagtgac
     1861 attctcattg tcctgtctga tattgcttcg tataaaagta ccagatccac gtctcgtata
     1921 taacaaacct tcatcaataa ggagttgtat agctttgcgg atagtaacac ggcttgtttt
     1981 aaactctcta gccagacttt cttgctcagg taatttagaa ccttcctgat attttccctc
     2041 aacgatcctc tcttttagag ccaaggctat ttctttatat tttggcatat tttcccctca
     2101 tttcattgat cctacaaaaa aattagt
//
LOCUS       DAZKNO010000246         2115 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000246 DAZKNO010000000
VERSION     DAZKNO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.252"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..1004)
                     /gene="cysK"
                     /locus_tag="ACG8MY_002628"
     CDS             complement(78..1004)
                     /gene="cysK"
                     /locus_tag="ACG8MY_002628"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0431763.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1144..2031)
                     /locus_tag="ACG8MY_002629"
     CDS             complement(1144..2031)
                     /locus_tag="ACG8MY_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0431764.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          584 a          473 c          356 g          702 t
ORIGIN      
        1 tcatcataaa tttttgtgtg ttcttttcat ctttaaaagt tttttacata aacctatctg
       61 gagaaaaacc aagtgcctta tatctcttgg taaagggctg tagataaata acgttcacca
      121 ttgtcgggaa ccacagctaa taccttcttg ccttttccta attcttttgc gacttttagc
      181 gcggcagcaa ttgcagcgcc agatgagatg cctactaaaa tcccttcttt tcgcccaact
      241 tctctagctg tttccatcgc ttcgtctcca ctaatagaaa ggactttatc atacacagaa
      301 gtatccagtg ttttaggaac aaaacctgta ccgattcctt ggattttgtg aggtcccgct
      361 tctttccctt ctaaaatagc agattctgct ggttctaccc cgattaattc gattttcgga
      421 tacacacgtt ttaattctcg gccagcgcca gtgatcgttc ctcctgtacc gatacctgca
      481 acaaacgcgt ccaagccatt tactccaaat gcttgatgta tctcaggacc agtcgtcttc
      541 tcatgcacaa gaggatttgc ttcattttca aattgtaatg gtaaaaagta gccgttttct
      601 tttgccaaac gttctgcttc tctgattgat ccagaaatac catccgcacc aggagtcaaa
      661 atcaactctg cgccgtaccc tttcatcaat aaacgtcttt cgatactcat cgtctcaggc
      721 atcacgatga ttactttata tccttttgca actcctacca tcgacaatcc gattcctgta
      781 tttccagaag taggttcaat gatagtatca cctggtttca gtagaccatc gtgttctgct
      841 ttttctatca tacttaaagc aatacgatct tttacacttc ctcctggatt aaaaaactca
      901 agctttacaa aaacatctgc tgaatcttct ggtacgattt tatttaattt gacgattggc
      961 gttttgccaa tcaattcggt gactgagtta tagatagctg tcatcttcaa ttcctccttg
     1021 tctttgctct tcttacttat cataatacaa tctgttcatt tatggcgagt gattcgtctt
     1081 tgactagcaa aaaaaagaca aagatatacc atatatggta atttctttgc ctttttatct
     1141 aaatcaaacg tttagttctt gaccaactaa taaagaataa ctggacatag acatgccatt
     1201 tttcgccatt agatcatcaa cagacatgcc aaatttttgt gcaatatccc atagcgtatc
     1261 acctgacacg actgtatatg tctgaccatt atttgtattt acatctgttg ttgaagatga
     1321 tgacgttgaa gtcgtcgtag tagtcgttgt tgttgtactt gtggttgttg tagccgaacc
     1381 tgttgatggc gtatcaaaac gggttaaatt ataagtatta atcaatgagt tcaatttggc
     1441 cccataattt ggatcggtag cataacgacc tgtcaagtac gctgttgcat cataaaaact
     1501 tgttgtattg cttttccaca cgccagcata atgataatcg cccgtggaaa aactggttga
     1561 acgaagaaca ttcgcatggt cttggaagga ttcccaataa gaagaatatt ttcggaatgg
     1621 ctcattcatc gttacccatt gtccgtttac atattctttg gttgccatat aaacgacctg
     1681 tccgttatag cttcctttca caccgaatag attataattt ggcgcactag ctaatccgga
     1741 agtgccatat gcactttcca acaacgcttg agcgatcatg actgaagcat acaagtcatt
     1801 acttgcagca acttctgacg cagaccagcc gatttgatcg ataaacgctt gaggatcagc
     1861 gactgtttga gatccttgtt catctgcatg aacatgttgc ggtaccgctg taaccatcat
     1921 cggcccaact aaaagactcg ttccaattaa aggagcaatc gcttttttat tgatcaccat
     1981 ttttctttta ggtctatggt gccgggcact gcgggtcttc agctcttgca ttcaaattcc
     2041 tcctcaaaat aaaaaaactt aataaaaagt tctcttctta aaacaaactt aattaattaa
     2101 attatacttt taatt
//
LOCUS       DAZKNO010000247         2113 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000247 DAZKNO010000000
VERSION     DAZKNO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.253"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(101..708)
                     /locus_tag="ACG8MY_002630"
                     /pseudo
     CDS             complement(101..708)
                     /locus_tag="ACG8MY_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1231..1905
                     /locus_tag="ACG8MY_002631"
                     /pseudo
     CDS             1231..1905
                     /locus_tag="ACG8MY_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            1898..2059
                     /locus_tag="ACG8MY_002632"
     CDS             1898..2059
                     /locus_tag="ACG8MY_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431765.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          758 a          330 c          362 g          663 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta aga
//
LOCUS       DAZKNO010000248         2105 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000248 DAZKNO010000000
VERSION     DAZKNO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.254"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(127..357)
                     /locus_tag="ACG8MY_002633"
     CDS             complement(127..357)
                     /locus_tag="ACG8MY_002633"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0431766.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(451..789)
                     /locus_tag="ACG8MY_002634"
     CDS             complement(451..789)
                     /locus_tag="ACG8MY_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431767.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1717..1833)
                     /locus_tag="ACG8MY_002635"
     CDS             complement(1717..1833)
                     /locus_tag="ACG8MY_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0431768.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT          687 a          375 c          281 g          762 t
ORIGIN      
        1 acaaaagtta aaataaaata ctctggatac tctttatact gcattaaagt tagtaatatt
       61 aacagcaaag atatcgaaaa aacaataaca taatatgtgt caaaattttt cataataaga
      121 actcccttac tgttgcatac taacataatc caaaaactct tctacttcag ctaaagacat
      181 tccactttct ttagtccaaa aaacagtttt ttctatcgat ccttctctgt aaactgtaaa
      241 tgtcggagtc gagttaattg agtagcgact cttgaattct ttccactcat tttcatcttc
      301 tctaactttt gctacattca cgtaataaat attttctttt cccttttcta ccagcatatc
      361 gaatagcttc ctgcttttga tgatcgattt cttgtgctac atctagtaaa ttttcatcaa
      421 tttctatatc ataaagtatt tcactagtgt ctacatccac aatatatact ttttatcagt
      481 agcaattttt gagtaatccg ttaaaatgat ctcagtctcg tcactttcat cagtataaat
      541 atacgttaac aatgaactca ccttttcaat cggtgttagc tctaagccta ctgtattttt
      601 tactattgag tcattattta ccaccataac ttcgtactgt atataccatc ctatttggtt
      661 tccatcaata tcagtaattg gctcactagc taatggagtc tccggaacaa tattttcctg
      721 atatttctta aactcttctg tagcacgaaa aaggttatca tgttcctcac ttgaaactgg
      781 ttgagtcata gaaaatgtta cttggctaaa cagagggaaa ataaacacta caatcaacaa
      841 agtataaaaa aaagcccttc tttttcattt tttatcttcc aatcacaaat tgaaaaactt
      901 attcctgcaa ctttgagtct agtatcacaa tcgactgact acaactattc atttacattc
      961 tactttaagt tcctccttaa ccattaattt tattaaagat acttgtaata gtgattctca
     1021 caaaatttca tgaaaaatac tttattactg tatctaaaat tccaatacac aacttatcga
     1081 ctattatctg aaaaccaaaa cttattattc cttgatgtta ttctagcaat agtaactgct
     1141 tttttatttt tttgcatatt ttttcggcgt tactttttaa agttaacttt ttcgacaaat
     1201 agaaagagat acgaatcact gatttttttg aagaaaagaa atggagtgag ctgaacgtta
     1261 gagatattgg gagctttcgt aactgcttat taaacgataa tggttatttg caaccgtatt
     1321 gctcgcttct acatgaaata tttcatgaaa tatttcgcaa aacctttgat tatgtagtta
     1381 cgtttccatt cttgactaaa aatgtttcaa aaacgaacaa aggtgttaaa aggtaaagtg
     1441 atcaatacta ctggtcaaaa taagaggttg aaagggaatt gaaaaataac tacagataat
     1501 acttttataa acgtacgtgc tttgtggttc tgtagttaac tatataactg gaattaggga
     1561 atggtatcaa tttgaaaaga aagattctac caatttatca tatgttaaaa atggaaaatc
     1621 aacagcagtt ctttcggaaa ccttacacaa aaaccgatta ttgaaaatag ccgaaaaacg
     1681 tttttcggct atttttttaa ttaagtttcc ttcttcttac cttttcccac tattccgttt
     1741 cacatctttg atgatcttca gcgcacgttt tcgtgttttg atatttgggc ttttcaagcg
     1801 gcggtatgca cttgagatat ctgttttttc catatggacg acccactttc ttctttgatt
     1861 gtagtcatgc taaaaaacac aaatcgaaac aattacgatt tgtgttttaa atggtatcaa
     1921 aagttaaaat gaaagtcaac ttgttaaaac agtcgatcag actagctgat tgactcctat
     1981 ctctggaaat aaattttcat tattacgttt tagctaccta aaaaaggccc tcttgtccgt
     2041 taactttaat atctattgtt atagctagtt tacctattga acgtactgtg tgttcgatag
     2101 aacac
//
LOCUS       DAZKNO010000249         2056 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000249 DAZKNO010000000
VERSION     DAZKNO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.255"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            493..714
                     /locus_tag="ACG8MY_002636"
     CDS             493..714
                     /locus_tag="ACG8MY_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431769.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="ACG8MY_002637"
     CDS             715..972
                     /locus_tag="ACG8MY_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431770.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="ACG8MY_002638"
     CDS             complement(1108..1710)
                     /locus_tag="ACG8MY_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0431771.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="ACG8MY_002639"
     CDS             complement(1777..1932)
                     /locus_tag="ACG8MY_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFQ0431772.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DAZKNO010000250         1999 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000250 DAZKNO010000000
VERSION     DAZKNO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.258"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..127)
                     /locus_tag="ACG8MY_002640"
     CDS             complement(<1..127)
                     /locus_tag="ACG8MY_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0431773.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKT"
     gene            764..850
                     /locus_tag="ACG8MY_002641"
     CDS             764..850
                     /locus_tag="ACG8MY_002641"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0431774.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(971..1660)
                     /locus_tag="ACG8MY_002642"
     CDS             complement(971..1660)
                     /locus_tag="ACG8MY_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431775.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1667..>1999)
                     /locus_tag="ACG8MY_002643"
     CDS             complement(1667..>1999)
                     /locus_tag="ACG8MY_002643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431776.1"
                     /translation="LRLNNRKLDRLEETMTNTLKEEQTTNKNYRSKTLQDIENEIKKQ
                     RAFLAPYTKERIQTTIDSQVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLL
                     AFWLVWGE"
BASE COUNT          582 a          379 c          332 g          706 t
ORIGIN      
        1 ctgtcttatc ccctttttca cgcattctag acacgatgga aaaaaacaaa ttcatttcaa
       61 ccggtgtaaa ttttcttaaa ggaattgtat ttaattcatg gtggtatttt acaatctcat
      121 tagccatata atcacctcaa tccaattatg aatgacattt tttaaatgtc aagacattca
      181 tatttgacgt tcaaacagac aaaatgtcct tcataaacag acaaaatgtc cttcataaac
      241 agacaaaatg tccttcataa acagacaaaa tgtccttcat aacattctgt atccattggt
      301 agagtaagga cacagcttac ggtaaagagg tttaaaaaga ggtttaaaaa gagatataaa
      361 aagagggggg ctaattttcc cgcctaaaaa ccacttcttt tttctttaaa caggttcgcg
      421 catttttcaa aactgccaat ccaaaacctt gcttcctacg gtcgccaaaa aggtgtgatc
      481 gctacgctct cacattgtac tgcgcagtcg cctaaaggct cctttgctaa aaacatgttg
      541 gtaaagaggc ctttgagcgg ttctgaatgc ttatctaagc cgttttaaga cagtcttgga
      601 taaaaatcct tatctagctt taaagcgcct tacaggcggt cagggcaagc cctaacaacc
      661 ctaaaataag cgatacttaa aaacaccaca caagtttaat tttttaagat ataataactt
      721 gtcgcctatc cgacagtctt gcgactggaa ggaggtgtat ctaatgtttg aattgttcat
      781 ttgccccctt cttatagaag tttgtgtctc tttattcagt tattggttga ataaccgaaa
      841 caagaagtaa aatcaataaa ggcgacatca acctgaaaaa aagccccaga gaatccaacc
      901 tctctggggc tttcgtgtat ctaatgtttg atcatttgca tgtttagttt aacatgattg
      961 ccgatcaaat tcaattttgc tgttgctgat ctaaccattc cttgacactt tttagataat
     1021 ttaatgccgt ttcttttttc gttggatttt gtatgttcgt tgcttgctga agcagtgctt
     1081 tataacgttc gtaggtaact gattgttgcc gttcttttcg ttcctgttca ctagttgaaa
     1141 aagggattgt atcactgaat aaattataac aagcttcttc tgtctgcgta tctttaaaat
     1201 attgcacgtc ttttttggtt cgctcatagt tgtcttgaac ctctttttgc tgtttttctg
     1261 gcaagttttg ataaagacga gtgagttttt ccatgttttt atccgtcaaa cgtttttctt
     1321 gattcacaaa aatatcgttc atttcctcat aacaagtggt aaactcttcc attttttgtg
     1381 ttaacacttg ctcctttttc tttttatcac atgctgcttc atacttttga gtgatatccc
     1441 ctaataaaat acttcttgaa atacgtttat ttccctctaa aacgccgaat aaaccactta
     1501 aaacgattga taatgtaaat ataacccctg gcaataaaaa cctcttaaat cgaattttag
     1561 agccttttaa tgaatatctt gtttgataag ccttatagct aagccaaata attcctaaaa
     1621 tactggcaaa aatgagtaga tagggtaaat agaatgccat ttctatttat tctccccata
     1681 ccagccaaaa ggctaacagt gtcatgatta agcaacataa gggggcaaga atatccattg
     1741 atcgctgcat aattactttt ttctgttctt tatttcgctg gtttaatagc gcatggtgga
     1801 attttttgac ttgtgaatca attgtagttt gtattctttc tttggtataa ggtgcaagaa
     1861 aagcacgttg tttctttatt tcattttcta tgtcttgtaa cgttttagaa cgataattct
     1921 tgttggtagt ctgctcctct ttcaacgtgt tggtcatggt ttcttcgagt cggtctaatt
     1981 tccggttgtt cagtcgtaa
//
LOCUS       DAZKNO010000251         1985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000251 DAZKNO010000000
VERSION     DAZKNO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.259"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..298)
                     /locus_tag="ACG8MY_002644"
     CDS             complement(134..298)
                     /locus_tag="ACG8MY_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431777.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            593..1921
                     /locus_tag="ACG8MY_002645"
     CDS             593..1921
                     /locus_tag="ACG8MY_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0431778.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
BASE COUNT          666 a          326 c          391 g          602 t
ORIGIN      
        1 taaagcttat gcaaagaaaa agaaatccaa tcacttgggt ttctttttct tttattatta
       61 tgatgaatca agctttctgt ttttgtcacg tatgttgtaa tctttacgaa agagtagagt
      121 tggctactca taatcaacca tacttcccat catcgctaaa tacgaaataa atcagcattc
      181 ctacaccaat caaagcacaa gctgcaccaa gaaaagggat gacattccca aagttatctg
      241 tattcataag tatgccacca cccacaaaca aaaagccgcc agcaatcagg tttttcattt
      301 tattcatctc ctttcttacg ttatagtatt tccttctttc aatgagtatt ttgaaagcta
      361 tcttattgta tgatgttctt ctaggcctct tctttattat acaggccatt ttcgttatct
      421 catagaaata ttccttttta tctgaaaact attgtatacc gtatgacatt tttcttcatg
      481 aagttgtaag tatattctca ttcaaatgaa aatttcatca tagaagagat tgtatttcta
      541 aattcttaag agtaaaatac gaagtataac ttggatttgg aaggatgatt ggatgaaagc
      601 tattacgcct gatatgacaa caaaatttaa caaacagttt caagcaaata tcaaacagca
      661 ggcattgcag cgggcagttg tgaagaacgg aattactgct tctgctgaaa atgtttcagc
      721 acatgtggag aatacaccgg tattttctat agatttggct acggggaaag tggctaatca
      781 aatgcaatcg gggcgttgct ggatgtttgc tgcactgaac acattccgtc ataagatgct
      841 gaatcaattc catttaaaag agtttgaatt atcacaaaat tacacttttt tctgggataa
      901 atatgaaaag gcaaattatt tctatgaaaa catcattgca acaggaaaag aggcaatcga
      961 tagccggaaa gtagctttct tactcgctac tcctcaacaa gatggcggcc aatgggatat
     1021 gatcgtttca ttattccaaa aatatggcgt tgtgcctaaa tcggtgatgc cggaaagtgc
     1081 gaatagttca aattctcgag atttgaataa ctacttgaac aaattactgc gtaaagatgc
     1141 agtagtgtta cggaaaatgg ttgctcaagg agaaacatta gacgctattg aagaaaagaa
     1201 agaagaaatg ctagaaagca tttataactt cttagctatc tcattaggaa cacctccaaa
     1261 agaattcgat tttgagtacc gcgatgaaga aaagaactat catcttgacc gaggactgac
     1321 accacaaatt ttttatgata aatacatcgg tgtaaaatta gatgattatg ttagcgtgat
     1381 caatgcacca acaaaagaca aaccattcaa tcgttcttat acagtagaga tgctaggaaa
     1441 tgtcgttggc gggaaagaag tcaaatatct gaatgttgat atggaaactt tcaaaaaatt
     1501 agcgattgct cagcttgaag aaggagaatc tgtttggttt ggttgtgatg taggtcaatc
     1561 ttctactaga actagtggca tcatggcctt agatgcttat tcaatggatg atctatttga
     1621 tacggatttt acaatgacta aagctgaacg tttagatttt ggagaaagtc tgatgaccca
     1681 tgcgatggta ttgaccggtg ttgacttagt agacgggcaa tcaacaaaat ggaaagtcga
     1741 aaatagctgg ggcgaaaaag ttggaaagaa tggcttcttt gttatgagtg atgcctggat
     1801 ggacgaatat acgtaccaaa tcgttgtaag aaaagattta ttgacgaaag agcaattaaa
     1861 tgcgttcaat gaagaaccaa ttgttctatc tccgtgggat ccaatgggtg ctttagcata
     1921 gacgagtatt tcaatgaaga gtatgtagta aagataaaaa gagctggctt aagccagctc
     1981 ttttt
//
LOCUS       DAZKNO010000252         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000252 DAZKNO010000000
VERSION     DAZKNO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.260"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG8MY_002646"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG8MY_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG8MY_002647"
     CDS             complement(728..1744)
                     /locus_tag="ACG8MY_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0431779.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          559 a          459 c          308 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cgtaaatcca cgtcactaaa cattgacgta aatttactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKNO010000253         1967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000253 DAZKNO010000000
VERSION     DAZKNO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.261"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG8MY_002648"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG8MY_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0431780.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG8MY_002649"
     CDS             852..1040
                     /locus_tag="ACG8MY_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0431781.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG8MY_002650"
     CDS             1053..1610
                     /locus_tag="ACG8MY_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0431782.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG8MY_002651"
     CDS             1642..1884
                     /locus_tag="ACG8MY_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0431783.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          718 a          305 c          404 g          540 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatat
//
LOCUS       DAZKNO010000254         1902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000254 DAZKNO010000000
VERSION     DAZKNO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.264"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1902
                     /locus_tag="ACG8MY_002652"
     CDS             <1..>1902
                     /locus_tag="ACG8MY_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0431784.1"
                     /translation="NPIADNLRYELVENSNNEFVATKFSDNFLSTFSDSLKSGYYRYQ
                     ANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLVFATDIETGQRSKVVLSD
                     ILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIAYICAVFVLIKGSGTVMR
                     YFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGEGEDRKPEKNFGETLSKK
                     AGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDIANDTKNKFKEGLDDGTV
                     SAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVENADRIMSSSERMREAMKN
                     NAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQKRHEAKYTPEAMNREEIM
                     LRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKENPQNSTSSHGGRSVDVRE
                     NIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSSSTSQPKTVDVRENVQNS
                     TSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGFKTVDVRENIQKDSGEMK
                     EKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSRRRFTYEDNELFRDTLND
                     PNPLFDNLL"
BASE COUNT          712 a          278 c          406 g          506 t
ORIGIN      
        1 aaatccaatt gcggacaatc ttcgttatga attagttgaa aattctaata atgaatttgt
       61 tgctacaaag ttttctgaca acttcttatc aacattttct gatagcttga aatcaggcta
      121 ttaccgatac caagcaaatc tatgggggat ttcaattggg ttgacagcgt tagcaatcgc
      181 ctatgtgttc agcgcgtttg tgatcattac agcaatacta gaacttgcat ttaaacgtgt
      241 gttaggtgtt cttgtttttg caacagatat tgagacagga cagcgttcaa aagttgttct
      301 atcagatatt ctacaatgtt acttaactgt tgggtttcaa ggatttgggt tatcaatgtt
      361 tgctatgttt atcaattttc tgaactcagg acaaggtatc tcaacaaata tttttataaa
      421 aacaattgct tatatctgtg ccgtttttgt tttaatcaaa ggcagtggaa cagtcatgag
      481 atactttggt gttgatattg gactcaaaga aggctatggg caacttgctt ctgcttttgg
      541 aatgggcgca atgctgttta gaaaaggatc aaatggattt aatcgtgcta aaggaagcgg
      601 agaaggagaa gatcgaaaac cagaaaagaa tttcggcgaa actctatcaa aaaaagctgg
      661 caaaactgga agagctttgg gatatgccca tgaacgtgga ttatccggtt tagcttcaga
      721 tggagctaca atggctagtg aaagagcaac aaaaccattt aagagtatgc gtgatatagc
      781 taatgatacc aagaataagt ttaaagaagg tttagatgat ggtacagtgt ccgctattaa
      841 taaaaatagc aaacctatgt tagctaaaaa taaagaagat gaaactggaa aatatgctga
      901 ttccgtgcca acacgtcttt cagatagaaa agatggtgct aaagttgaaa atgccgatag
      961 aattatgtct agttctgaac gtatgcgtga agcaatgaaa aataatgctg aatcgaataa
     1021 taatcctgca agcgcaattc agcaaaaagt ccaacaagat attgaagaac gtaaaaatgc
     1081 tatgcacggt caagctaagt ctgctgaaga attaattaat cagaagcgtc atgaagcgaa
     1141 atacacgcct gaagcaatga atcgcgagga aataatgtta aggaaacgtg tagaagggcg
     1201 gacaggcgct aatattgacg gaaaagaagc gttgaccaaa gaaagaattc aagaagctaa
     1261 aaattcaaat acaggattag aaaaattagt caaagaaaat ccacaaaatt ctacaagtag
     1321 tcatggtggc cgatcagtag atgtccgaga aaatattcaa ggttcactga atggtcatgt
     1381 tggacggaat gttgatgtaa gagagaattt acaaagttct tcaaatgctc aaggtactaa
     1441 gacaattgat gttcgagaaa atctgcaaag tagcagtaca agtcaaccaa aaacagtaga
     1501 tgttcgagaa aatgtacaaa attctacaag tagtcaaggg tttaaatcag tggatgtccg
     1561 agagaatgtg caaggtagca gtgcaagtca accaaaaaca gtagatgtcc gagaaaatgt
     1621 acaaagttct acaagtagtc aaggattcaa aacagtagat gttcgggaga acattcaaaa
     1681 agatagtgga gaaatgaaag aaaaaacaca aaaaatcaat atcgttgaag acagaaagtc
     1741 agcgaaaaaa tttgattcaa atcatgaaac agtgattatt gatagtgaaa tccgtgaaaa
     1801 tgataaggga tcaaaatcaa gacgaagatt cacttatgaa gacaatgagt tattcagaga
     1861 tacacttaat gatcctaatc cacttttcga taatttatta ag
//
LOCUS       DAZKNO010000255         1896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000255 DAZKNO010000000
VERSION     DAZKNO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.265"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..294
                     /locus_tag="ACG8MY_002653"
     CDS             <1..294
                     /locus_tag="ACG8MY_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HFQ0431785.1"
                     /translation="SGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSP
                     IFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            422..1105
                     /locus_tag="ACG8MY_002654"
     CDS             422..1105
                     /locus_tag="ACG8MY_002654"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0431786.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1414..1487
                     /locus_tag="ACG8MY_002655"
     tRNA            1414..1487
                     /locus_tag="ACG8MY_002655"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1448..1450,aa:Arg,seq:tct)
     gene            1646..>1896
                     /locus_tag="ACG8MY_002656"
     CDS             1646..>1896
                     /locus_tag="ACG8MY_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0431787.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSML"
BASE COUNT          636 a          303 c          396 g          561 t
ORIGIN      
        1 tcaggaaaaa aaatctcgac ccgtcgcgta atagccgttg aaaacaacca cgaagatttt
       61 tatacaaaag ctgatacaaa gacttatatt gagcctggag tagtaaaaaa gagaaatatt
      121 atcggtagtc ccatctttca gattccatat attggactag tcctttcaaa aacggcattt
      181 gcatggattc aggggttatt ctttacgatt gctttttgcc taacaggaat cacagcatgg
      241 aatacctttg tagagttgaa aaagaagcgg agacgaacac agaaattttt gtaagaatgt
      301 taaaaacata aatgtattcc agagatccaa cgactcttct gaacatgaaa cacgagaaaa
      361 cactcatgca gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga ggaggagtag
      421 tatgcgcaat gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg attttatgcg
      481 tacccagcaa aaaaaattac cgggtgagct aggcaaactg gaagaagaag ctcatgtagc
      541 agaagtacca gttattcccc atgaaacagt tgtatttttg aaatttttac ttggacagct
      601 tcagcctgaa cgaatcttgg agatcggagc agctatcggt ttttcatcaa gtgtcatggc
      661 cacgacaatc ggagaaaacg ctcatgttac gacaattgat cgtttcgatg tcatgatcga
      721 gaaagctaaa aaaaactatg aacgtctagg actgacagat aaagtgactt tgcttgaagg
      781 tcaagcagca gatatcttgc cggagttgtc tggtccgtat gactttattt ttatggatag
      841 tgcaaaatca aaatatattg aatttttacc tgaatgctta cgtttgttaa gaaaaggtgg
      901 cgttttgatg gtagatgaca tcttccaagg aggaacgatc ttgctcccag atgaagagat
      961 tccccgtggc aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg ttgtgatgga
     1021 tcatcccgat ttgacatcga caatcttgcc attaggtgat ggtgtgatct taatgacaaa
     1081 ggaatcagaa acaatcgagt tgtgatacat gattatggca gcctgtagat aatcgattga
     1141 atcgagggct caattcgaac ttgatgtaaa agtttctata tttgtaagtc aattaaagga
     1201 tacaggaaat ctaaataaac aggagtagca tgaagtcaat gtagctcctg ttttcaattg
     1261 ataaccttag aacagctgaa ggagaatgtc tcgttttaaa agattgattt agtagttggg
     1321 ccaaaataat tcattatttg ctaattaaat aactatttcg tattgacaga agcaatttaa
     1381 ttatctataa ttattgatgt tccgctgatt cttgtcatag tagctcagct ggatagagca
     1441 tccgccttct aagcggacgg tcgggggttc gaatccctcc tgtgacgcta aaaaaccgtt
     1501 agaaactgag gtttctaacg gtttttcttt gttttttgtg ccaatattgt ttgttttcaa
     1561 aggcaaataa aataaaaaaa catccttatc gagtacaatt ataaggagca aaaaaataaa
     1621 actcatcaga aaagaggaag aagatatgac gatactatct agaattttag tgactctagt
     1681 tgcaattgaa tttttttata tcatgtatat cgaaacggtg agaactgatt ctgacacgac
     1741 aagtagggtt tttaaaatga gtaaagaaga gttaagcaga aaatctgttc aaacattgtt
     1801 taagaaccaa ggagtgtaca atggactatt aggagtggga ctactttatg gagcttatct
     1861 ttcttccgca tctaaagaaa tcacaagtat gctatt
//
LOCUS       DAZKNO010000256         1886 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000256 DAZKNO010000000
VERSION     DAZKNO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.266"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..1097)
                     /gene="queA"
                     /locus_tag="ACG8MY_002657"
     CDS             complement(66..1097)
                     /gene="queA"
                     /locus_tag="ACG8MY_002657"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0431788.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1290..>1886
                     /locus_tag="ACG8MY_002658"
     CDS             1290..>1886
                     /locus_tag="ACG8MY_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0431789.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT          541 a          394 c          351 g          600 t
ORIGIN      
        1 ttttctttgt cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca
       61 taaccttatt tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc
      121 gcatgctggt aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact
      181 aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag
      241 ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc
      301 gttccaatcg tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca
      361 tttttacgta cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta
      421 tgcatatggt gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca
      481 tgtagtgtca gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc
      541 gtaaaatgca atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt
      601 tgatagcgat ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc
      661 aatgattcaa gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg
      721 tgttccaatt cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc
      781 ccaactttgg cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc
      841 gttagaagaa ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca
      901 ggcagaactc gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt
      961 tcatggaaat gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa
     1021 tgatcgcggt cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa
     1081 tcttctgttg ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg
     1141 tttttcgttc tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata
     1201 aaaaaactac cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct
     1261 tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt
     1321 ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca
     1381 tttgttttat cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa
     1441 tgactgatcc aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc
     1501 cagtggaatt gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca
     1561 tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac
     1621 ggaataaaac agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc
     1681 gctacccaca tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg
     1741 cagcgaatca agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc
     1801 gtgattcctt gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat
     1861 ttgtaaccca tgacatggat gaagcg
//
LOCUS       DAZKNO010000257         1816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000257 DAZKNO010000000
VERSION     DAZKNO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.267"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..1729)
                     /locus_tag="ACG8MY_002659"
     CDS             complement(143..1729)
                     /locus_tag="ACG8MY_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0431790.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT          609 a          371 c          336 g          500 t
ORIGIN      
        1 atacacggtg ttccagaagt aacttcttcg gaaataagcc gaaatttcac aaaaatttga
       61 aaagcaattt tcttaaattc cttcttattt cttgaagctg cccacttctg tcacaacctc
      121 ctttgtgtaa aagaatcagt ttctattcga tttcttctat cgtttcttgc aaacgctgtt
      181 catatttttt gtttccttta tataaatcac caatcgtcca tgccaacagg agaaggacaa
      241 gaacaatgat tacataggcg atttgatcag ccaatgctag ttgagctgag gtggcttgct
      301 cgccgaagaa actttcgatt tgacctggta atcctcgtaa gttcaagtaa gtcagactaa
      361 tcacagagaa ccagccaaga agttttacaa caaatgaatt tttgaatcgg ctccccatct
      421 ccgcttggct attggtcatc atcagtaaag gaatcatcga gaatggcaat gcaaaagcca
      481 gaaaaacttg ggattcattc atcaagttat tcaaagcggt atgttcttcg atcgttcctt
      541 ttgtacttgt aaacaaaaca caaatcaata caggaatcac agaaagcaat cgtgtgacta
      601 gtcttcttag ccaaatcggg atacgcatat ggatgaatcc ttccatgatg acttgaccgg
      661 tcaacgtacc agtaatagta gagttctggc cagaagcaag aagtgctaca gcaaataaag
      721 tagacaagat accagatttt gctacagcaa tcaagatacc attgctcata gctgatggat
      781 cagataaagc ttcatacaga ccaaaaaagg aaggatcttg gacagaaccg gttttgaata
      841 cagcgacacc catgatcaat agcagtgcat tgactaaaaa agccattgac aactggatat
      901 tagagtccca agtagaaaag cgtaccgctt gtgctacatc ttcttcgtct tggtgatcga
      961 tcttccgagt ttgagaaact gctgaatgaa gatataagtt atgcggcatg actgttgcac
     1021 caataatccc taacgctcca gtcagcggag tttcaccacc tatgacaggt gagtctgaga
     1081 atgtatgact gttagggata aagccagcaa ataaactgtg ccaatcaggg ttagacaagc
     1141 caacttgata gacgaaaacg aataaaatga cgaaaatcaa gcagacaacc aaagcttcga
     1201 ttttacgaaa cccgactttt gtcagcaata aaaggacgaa aacatctaac acagtgataa
     1261 atacggcaat taccaatgga atatcaaaaa gtaagtagag ggcgattgct gcaccaatga
     1321 cttctgcgat gtctgttgcc ataatggcta attcagtcaa gatccataaa atgacaccca
     1381 atgtcttact tgtccgtgct cggattgctt gggccaaatc catttgggtc acgattccta
     1441 attttgctgc catatactgt aaaagcatag caattagact agacatcaaa ataactgaca
     1501 tcaataaata ttggaaattt tgtccaccag tgatcgaagt ggaccagtta cctggatcca
     1561 tataaccaac tgctactaat gcacctggtc cagagtaagc aagaagtgtt ttaaaaaaac
     1621 tttttccctt tggtacatcc actgttccat ttacttcagc taaagataaa ccatttgtat
     1681 gttcaataag tcgatgctta tgaaattgtt tgttggattg attattcaag aaaaaaccac
     1741 ctttcctatt tatctacgat tacattctac acctcagaaa gaaaaaatta aagaaaaaga
     1801 ttcggtatgc ctaatt
//
LOCUS       DAZKNO010000258         1809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000258 DAZKNO010000000
VERSION     DAZKNO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.268"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..796
                     /locus_tag="ACG8MY_002660"
     CDS             <1..796
                     /locus_tag="ACG8MY_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0431791.1"
                     /translation="KDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFV
                     MFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLK
                     QYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKG
                     RGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQ
                     KKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            817..1350
                     /locus_tag="ACG8MY_002661"
     CDS             817..1350
                     /locus_tag="ACG8MY_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0431792.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            1365..1535
                     /locus_tag="ACG8MY_002662"
     CDS             1365..1535
                     /locus_tag="ACG8MY_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431793.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
BASE COUNT          763 a          313 c          304 g          429 t
ORIGIN      
        1 aaaagattta agtaactaca aagcaactgc aaatgtacga tttgtagaag atttaaaagc
       61 aacttatgaa aaaatcttat ctttacgctt tggtcgtaga agtgagtctg ggctttctta
      121 cgatatgttt gtaatgttta cagaatttaa aatcaacggg gacgtagaaa atccttacgt
      181 agatatacga atttacgaaa aagcactgcc tttgttaaat aatttagacg agtgggtacg
      241 ctattcttta caacaattca acgagctaca aagtggctat gcaaagacta tgtttcgtct
      301 gttaaagcaa tacagaacca aaggtttcgc atatttctcc aaagaagact ttcttgaatt
      361 attagatatt cctaaaagtt ataagcaacc tgatatagat aaacgagtta taaaacctat
      421 acgtcaagaa ctaactgcaa tcttcaaagg actaacaatc aaaaagaaat acggaaaagg
      481 acggggaaaa cctgttattg gctatcaatt tacgttcaaa cctgagatca aaaaagcaga
      541 cgactttaac aaacatgcac caaccaaaga agaccaaaat aaagcattgg ctttagctga
      601 taaacaaaaa gaatacgaag aacccgctac aacaattaac gaaaaaatca aacagaagaa
      661 acgagaactt gaactcctcc aacaaatcaa acaactagaa aaagagaaaa aagctcttga
      721 aaaacaaaca gagtccgcac tttcggaaaa agagaaaaaa agtttattat ctgatttaga
      781 taatttattc cagtagcaac ctaggaggca accagtatgc aaccacaaga aacctttaca
      841 ataaaggaat tgtcagatct ttttaagatg agtaggcaag caatatcgaa acatatccaa
      901 aaattagatt cttccatgat agctaaaaat gagcgtggtt ataaggttgt attgcgttct
      961 ggagtattgc aacttgctcg caacttagac aatcaaaggt tgctagataa gttgcataaa
     1021 catgaacaaa acaacgataa aaatgcttat ataccaaaca aaaacgtggt tgcaaacaag
     1081 ttgctagatc agttgcgtga agaaaatgaa tttttacgag aacaacttgc cctcaaagag
     1141 caaaataatt ctcatataca aaaactactc gaccagcaac aacaactcac cctgcaagcc
     1201 aaccaacaaa tcgaaaaact tcaggagcaa ctgcagttga cttatacgga agagtcttca
     1261 gagaatagtt caacgactct ttctgaagca acagaaacaa tggacgaaca aacaaagaat
     1321 tggaaaaaat ggtggcagct atggaaatga agaaggaaat acttatgaca aaaaaacact
     1381 tgctattcgg ttctatccta ctaggaatat taagctttag cccaatgtta ttatcagtgg
     1441 ccaacaaagg aaataaaaaa ggggaactaa aactcaatcc taattactac aacagtcaaa
     1501 atgaggaagt atttgtagaa aaacttgata aataagaaaa gactgctcgt acgagcagtc
     1561 tttctgaaag agattatcct attattaacc ttagaaaaga ataataaatg aatatgagat
     1621 atgtaaaaag cataacaaac acaaacaaaa ataacaagta tttaaaaaat gaccgatcaa
     1681 tattcacaaa gaattagaaa gaaacaaccg cagaaaacca agccttaaaa aaagcttttg
     1741 acctagacca atgaaaaatc acaaagtgag cagctatagc ccttgataga ggggctactt
     1801 ggcatagtc
//
LOCUS       DAZKNO010000259         1755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000259 DAZKNO010000000
VERSION     DAZKNO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.269"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..231)
                     /locus_tag="ACG8MY_002663"
                     /pseudo
     CDS             complement(148..231)
                     /locus_tag="ACG8MY_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
     gene            580..1665
                     /gene="xerS"
                     /locus_tag="ACG8MY_002664"
     CDS             580..1665
                     /gene="xerS"
                     /locus_tag="ACG8MY_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0431794.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          649 a          299 c          246 g          561 t
ORIGIN      
        1 atatgcttct atcattacaa gctcgtttgg tgtaagatgg gtataagtca tttatgttca
       61 ctctccttgt atgctttagc gggtattaca atttgagtgt aacataaatg gcttttttat
      121 ttgtctcgct taattataca aacggcgatc taaaaaaatt gtatctccag gatgcaaccc
      181 attttcttta agaaattcct tttaaattgt taatactact gagtgcccca cttctctgaa
      241 ttttctttaa cttctccctc ccctttttta tttgtatata cattcatata cgcttgttaa
      301 tatcaaatca accgtagtct ctcttatgtc taattatata gaaagattgt tctttctata
      361 taatttagac aatttaaaaa attaagattg cctccttctg tttcctttcc tattcatgta
      421 taccatttat tttaaattat taaatctata cctatttatc tgcttttttg aaataaatta
      481 ataaatttca aagtatacca attgacaaaa cactctataa ttggtatagt ccaattatag
      541 agatactcct ctcccctact tatcgaaagg atgaataaca tgaaaaatac agaaatatat
      601 caaaaaatag atacaatcat acttcatatg cccgattata taaaagattt tgttcaagat
      661 cgtgaggata aagatcaatc acctcgaact actttagaat atctaaaaaa ttataagctc
      721 ttttatgaat ggcttctgag tgagtctatc gttcccgata cttcaatcac ctcgataaga
      781 cagttaactg cctatgatct aaacttgtac aaatctcatt tgaaacgtcg cacaaaggaa
      841 aatgtaaaaa aagagacaac aaaaactaag ctcaaagata ataataactt aggtctatct
      901 acttctacta tcaatagaaa tatcacagca ttaaaggttc tatttaaata tctttcgaaa
      961 agttccaata atcctttagg aaaaccatac ttagaaaata atcctatgga tcaagtagca
     1021 actatcacag ataaaactac gttagctgcc cgtgcaaacg caatcgagaa aaaattattt
     1081 ctagacgaag atacacaaaa ttatctagat tatattgcca atgaatataa aaaaactttg
     1141 agcaaacgag ctttgatcta ttatcaacga gatgtagaaa gagacctggc gatcaatgca
     1201 ttgattttag gatctggatt aagactctcc gaggttgtaa atattaattt agaggaccta
     1261 tctttagaaa agaataatgt ggtagtaact agaaaaggaa ataagagaga cgcagtaaat
     1321 atagcagcat ttgcaatgga atatttagct aattatctag ctattcggaa agaacgatac
     1381 aaagtaacag aaaacgaaaa agctttattt ctagcaattt atcaaggaga agccaagcgt
     1441 atatccggta ttgcaatcga acggatggta gcaaaatatt caaaaggatt cagagtccaa
     1501 gtcagtccac ataaattacg tcatactttg gctactagac tctatcaaca gacaaattca
     1561 ttggtattaa ctgcccaaca attgggacat acaagtacaa atacaacaac tttatatact
     1621 catatagaca atgcagctac aattgatgct ttgaacgctt tgtaaatgta gtaaaaaata
     1681 gtataataat gaagcctgtc tctaaaatta gataaaagcc caactttaaa ggcaagcttc
     1741 attatcattt ttata
//
LOCUS       DAZKNO010000260         1750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000260 DAZKNO010000000
VERSION     DAZKNO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.270"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(116..1264)
                     /gene="tnpB"
                     /locus_tag="ACG8MY_002665"
     CDS             complement(116..1264)
                     /gene="tnpB"
                     /locus_tag="ACG8MY_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFQ0431795.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1281..1685)
                     /gene="tnpA"
                     /locus_tag="ACG8MY_002666"
     CDS             complement(1281..1685)
                     /gene="tnpA"
                     /locus_tag="ACG8MY_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0431796.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          525 a          300 c          277 g          648 t
ORIGIN      
        1 gaagtgggag cttcttggga atagagcata cttgatagcg tatttcttta ctaacagcgg
       61 tgtctcttat ttaccaagct atccccgtag ttcctacggt tcttgatgat tggatctaag
      121 ccaatcccat agaatgtagt cttagacctt cggtcagaat attgatacta gcattgatat
      181 cacgatcatg atgagtatga caaacaggac aagtccattc tcgaatttca agagatttct
      241 tgccatcttt atgtccgcac tctgaacaaa tctgactaga cggaaaccat ttatctactt
      301 tgatgatttt tcgtccatac cagtcagcct tgtattgtaa tttagtcaca aactttgacc
      361 aagaaacatc agaaatactt ttagctaatt tatgatttcg caacatacct tttgtgttta
      421 agtcttcaat acagataata tcgtgatttt tgatcatttc tgtactcaac ttattcagaa
      481 aatcagtacg ttgattcatt actttttcat gcaatctagc tactttgcgt ttttgttttt
      541 gataattttt agcttcaaat agattggtac ctttattttt agccaataaa gcacgcctag
      601 acaatttgcg ttgttcacgc tttagtttct tttccatttt ggacgtgaat ttattattat
      661 caattttttg tccatcagaa agaatcgcaa agtccataat acctaagtca ataccaattg
      721 tggaattagt tttaggtaat tcgataattt cttctttaca caacaaagaa atatagtatt
      781 taccgctaga atgacgtgat attgtagcag atttgataat cccttttggt tgtctatgaa
      841 gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta tcaatcaaag
      901 caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc ttacttttga
      961 aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta tctaaattaa
     1021 gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat tctttcttgt
     1081 atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact tcttcatacg
     1141 ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca aaagatttgg
     1201 caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct tttagttgtt
     1261 ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat atacttctta
     1321 atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga ccagaacatt
     1381 tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct tgagctggca
     1441 cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt gaacataata
     1501 tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga ttctgaaatt
     1561 ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg tctacgatat
     1621 ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt tgtatctaat
     1681 tccatgttat atcaactcat ttcttgtata gactgattgt aacatggaac aaaataaaaa
     1741 ggcaatacgc
//
LOCUS       DAZKNO010000261         1750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000261 DAZKNO010000000
VERSION     DAZKNO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.271"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            383..1633
                     /locus_tag="ACG8MY_002667"
     CDS             383..1633
                     /locus_tag="ACG8MY_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0431797.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          578 a          335 c          308 g          529 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaataacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
     1621 gatagagaaa taacaatccg ttattgacgc aaatccttga atcaacccga aaaaaatttg
     1681 attttgatcg ggtctagttt cgtgcgcttc aaaacatatt actcaataaa gaaaatctta
     1741 aatttcatct
//
LOCUS       DAZKNO010000262         1737 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000262 DAZKNO010000000
VERSION     DAZKNO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.273"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1025
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8MY_002668"
                     /pseudo
     CDS             1..1025
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8MY_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1009..1401
                     /locus_tag="ACG8MY_002669"
     CDS             1009..1401
                     /locus_tag="ACG8MY_002669"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0431798.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1402..>1737
                     /locus_tag="ACG8MY_002670"
     CDS             1402..>1737
                     /locus_tag="ACG8MY_002670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0431799.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGI"
BASE COUNT          716 a          133 c          296 g          592 t
ORIGIN      
        1 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
       61 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      121 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
      181 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
      241 agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt ggttatgata
      301 gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga
      361 aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat ttagaaacta
      421 atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct atactaggtt
      481 ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg tcagaagaag
      541 aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac ggtttagatt
      601 atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa gagtatatat
      661 tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat atagaaagtt
      721 ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta tgccataatg
      781 attttagttg taatcatcta ttgttagatg gcaataatag attaactgga ataattgatt
      841 ttggagattc tggaattata gatgaatatt gtgattttat atacttactt gaagatagtg
      901 aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga aatatagata
      961 ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt gaaactattg
     1021 tttattgatg ataatatggt ttttgatatt gataatttaa aaggatttct taatgatacc
     1081 agttcatttg ggtttatagc taaagaaaat aataaaatta taggatttgc atattgctat
     1141 acacttttaa gacctgatgg aaaaacaatg ttttatttac actcaatagg aatgttacct
     1201 aactatcaag acaaaggtta tggttcaaaa ttattatctt ttattaagga atattctaaa
     1261 gagattggtt gttctgaaat gtttttaata actgataaag gtaatcctag agcttgccat
     1321 gtatatgaaa aattaggtgg taaaaatgat tataaagatg aaatagtata tgtatatgat
     1381 tatgaaaaag gtgataaata aatgaatata gttgaaaatg aaatatgtat aagaacttta
     1441 atagatgatg attttccttt gatgttaaaa tggttaactg atgaaagagt attagaattt
     1501 tatgatggta gagataaaaa atatacatta gaatcattaa aaaaacatta tacagagcct
     1561 tgggaagatg aagtttttag agtaattatt gaatataaca atgttcctat tggatatgga
     1621 caaatatata aaatgtatga tgagttatat actgattatc attatccaaa aactgatgag
     1681 atagtctatg gtatggatca atttatagga gagccaaatt attggagtaa aggaatt
//
LOCUS       DAZKNO010000263         1733 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000263 DAZKNO010000000
VERSION     DAZKNO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.274"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..552
                     /locus_tag="ACG8MY_002671"
     CDS             <1..552
                     /locus_tag="ACG8MY_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0431800.1"
                     /translation="KPIILKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIM
                     DLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLK
                     TNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRN
                     RIHSSIHYLTPQEFEELAKEKMA"
     gene            597..1553
                     /locus_tag="ACG8MY_002672"
                     /pseudo
     CDS             597..1553
                     /locus_tag="ACG8MY_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
BASE COUNT          619 a          283 c          340 g          491 t
ORIGIN      
        1 aaaccgatca ttttaaaaga gaatctctta aatcaggact tttctactga aacgatctgt
       61 gagaaatggg cagctgacat tacgtacatt cctactaaga aaaatggttg gtgttactta
      121 tcttcaatca tggatttaca cacgaaaaaa attattagtt atacattttc aaaacgaatg
      181 actgtggatt gtgtcattca aacattgaat aaagcaaaaa tacattatca cattccagaa
      241 ggaatgattc tacacactga cttgggcagc caatacacag caagagaagt ggaacaatgg
      301 cttaaaacca acaaaataag gcattcttat agtagaaaag gaacacctta tgataatgct
      361 ggaatcgaat ctttccatgc ctcattgaaa aaagaagaag tctacacgac tagctactca
      421 gattttgaag aagcaaatcg agcgctattt agctacattg aaggatttta taaccgaaat
      481 cgaatccata gctcgattca ctatctaacc ccacaggaat ttgaagaatt agcaaaagaa
      541 aaaatggctt aaccgtctct actaaaatgt gtccaagata ttgactcaaa tccattgatc
      601 gttacggtga tagggtaaaa aactggttta cattcaacga accaatcgtt ccccaaacgc
      661 gttgctattt ggatgctgtt cgctggccgc atgaacaaaa cacaaaaaag tggatgatgt
      721 ggaattatca taaagtttta gcaacggcta aagcagtaga gatttaccgc agcggaaatt
      781 atggtggaag gattggctgt attttaaatc ctgaaatggt ctatgctcga tctaattctc
      841 cagaagacca aaaagctgct tatatgtatg atttgtttta taatcgagta ttttttgatc
      901 cgatggtaaa agggtactac acagaagagt tcctggagtt gtgtcaaaaa caatcgattt
      961 atttcgatcc tgcaacagag gatttagaaa cgattcagca tcatacagta gatttccttg
     1021 gaatcaatca atattatcct aaacgcgttc gcgctcctcg ttatcaatgg aatgaagaga
     1081 cacctttcca tccagaacga tattatgaag tctttgattt acctgggaag aaaatgaata
     1141 attccagggg atgggaaatc tatccgaaaa tcatgtatga tatagccatg tatctaaaag
     1201 aaaattatca taatatccct tggctgataa cagaaaacgg tatgggaaga gaacatgaag
     1261 aacggtatat ggatgagaaa ggaatcgtgc aagatgatta ccgtatcgaa tttatcggac
     1321 agcatttgcg ttatttgatc aaagcaacag aagaaggcgc aaattgcgaa ggctatatgt
     1381 tatgggcatt cactgactgt gtgtcaccaa tgaatgcatt taagaatcga tatggattag
     1441 ttcgaatcga attgaacgat gaacgaagca gaacattgaa aaaatctgct tcgtggtatc
     1501 agactttgat tgataagaga gaattggatt tgcctgacga aatctataaa taaatagaag
     1561 ttttttgtag atgttcattg tatttactgg ttatttgaaa taaaagaggt tgtgaaaaag
     1621 ctgtcctgca tcaagtaata agaatagcaa agtaaaaaca acatcttatg tttttaactt
     1681 ggttggactt attaccgcag atgcaagctt ttgaataccg tttattcgaa aaa
//
LOCUS       DAZKNO010000264         1731 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000264 DAZKNO010000000
VERSION     DAZKNO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.275"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(367..1407)
                     /locus_tag="ACG8MY_002673"
     CDS             complement(367..1407)
                     /locus_tag="ACG8MY_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0431801.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          568 a          263 c          224 g          676 t
ORIGIN      
        1 gcatagcaga caattaaatt tatacaaaaa aatgtaagcg aataatattc gtattgcctg
       61 aaaagaagta atatcaagat ttttagcttg tttaaataat gaaattatct ctcttcgcgt
      121 attattataa ctcaaaaaaa aggagtatgc aaacatacgt tcccttttct atatttgaaa
      181 ttaattattt cttttaacac ttatgacgta aaaggctacg tttgtgattt ttatcataat
      241 attttaactt ataaattcta ttaaaatagc tattattttg taatattcgt aatataatta
      301 gaataataaa aaatttttaa ttttaatgtt actccatgaa gcaacaatca ctttgtcagt
      361 aaagtttcac tctaaccagt ctttcgtaat agtgcttggg gagctttcat aattcaaacg
      421 tttttcactt gatactttag cagctattgg tttttcgata aaccaatttt ttacatagac
      481 aaaaatcagg ttctggatat tttcgacttt ttcggttttt aatttttgat aggcttttaa
      541 cagtgtcgtg tataattctt gttctgcatt gacataataa ctatcgagtt cttcaaaaac
      601 tatcgaaata ccactttcta tttcagcttt tttctttgca ttatgaatag tctggacggt
      661 tttttgagct tcactgtaac ttgaagaaaa tgtttttatc aaatttaata ctcgttcagg
      721 gacaaacgta gcaatgctgt cgtttgtcat aattgtctca aaattgtcta acaacaattg
      781 atcttgtagg cggtcttttt cagtgtctat attgtgtcta ttagtgtcta atatattatt
      841 attaagttct ggtatgattt tcataccctc tgatttatca acgttttggc acggtatgat
      901 tttcataccc tcttgagccc caagggattc agcgtctgat ttttcgtcga gggtgggtat
      961 gattttcata ccctctgatt tatcaacgtt ttccctattc tctctatcaa atttttcgag
     1021 taagtagata tcattttctg agacttttaa ttctgctaga tatagtctat taggattatt
     1081 tttccctaac tgtttgttaa atcccatctg cttttgtaat aataaaccaa aggattccaa
     1141 ctcttttttg attgctatcg ctttttgttt ttggcagttt aatacgctac atagttcatt
     1201 tgtcgtataa gtaaaataaa cattaccttc ttcatcgatt tgccgtttgc tgattgcgat
     1261 ctctaatctt gcttttaaaa gcatataggc aatcttagtt gaatctttca tttttttgta
     1321 ttcgtcagaa acaataaaga cttttggaaa ttgataaaat aattcgttat aaactctatc
     1381 cgccttaaaa aaattaaaat cagtcattgt agatcctcta ctttcacgtt gttttattta
     1441 tgtaaaatta cctactcttt tcgaaaatag caaagataaa taaaaaatgc aaacgcatta
     1501 aaactgtttt ttaattaaat actacctaaa atcagaaata aaatgaatta tgtactacct
     1561 ttaaagtcat taagaactac tttaaataaa tattatttat aattaaatac taccagaaga
     1621 acgtatgaat aaaatttagt tttaaaatta aagataatta agtactacta gtgtcaaaac
     1681 aaaaaataaa ttaagaacta ccttatgcat ataaaacata ctaccattac t
//
LOCUS       DAZKNO010000265         1730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000265 DAZKNO010000000
VERSION     DAZKNO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.276"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..538
                     /locus_tag="ACG8MY_002674"
     CDS             <1..538
                     /locus_tag="ACG8MY_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431802.1"
                     /translation="VLANLIRNGMPKEGEMFNLDKIITWRENWSKNILGELEVGRVYT
                     NKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYKDKWLNGILQYTGMGLKGDQV
                     LDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYLAGQIYTVNEKDSSGNSRKVY
                     KFPLALINQEQLIEDKDI"
     gene            519..965
                     /locus_tag="ACG8MY_002675"
     CDS             519..965
                     /locus_tag="ACG8MY_002675"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0431803.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            1308..1631
                     /gene="relB"
                     /locus_tag="ACG8MY_002676"
     CDS             1308..1631
                     /gene="relB"
                     /locus_tag="ACG8MY_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0431804.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
BASE COUNT          657 a          227 c          316 g          530 t
ORIGIN      
        1 tgtattagct aatttaatta gaaatggcat gccaaaagag ggagaaatgt ttaatcttga
       61 taagattatt acatggcgag aaaattggtc taaaaacata ttaggtgagt tagaagttgg
      121 tagagtatat actaacaaag aaatttcaga gaagtttaaa tgttccaaac agggtggaat
      181 gagaagatct catcaaacta atactcttgt actgtttagt gatcaaacag gaagtaatgt
      241 ttataaagat aagtggttaa atggtatttt acaatatacc ggtatggggc taaaagggga
      301 tcaagtattg gataagaatc aaaataaagt tttagccaat tcaaaaagta attttgtaaa
      361 gattcattta tttgaaacat ttaaaccaaa agaacacaca tatctgggag aagtatattt
      421 agcaggacaa atatatactg ttaatgaaaa agatagttcg ggcaacagca gaaaagtata
      481 taaatttcca ttagctttaa taaatcaaga gcaattgatt gaagataaag atatataacc
      541 aggaagaaaa tcaaactaga catattcgta atttatctaa tgctaaatta gaggaagaag
      601 caagaaaagt tagtaattat aataaaaaaa tggctgcaaa atatgttaac gaagaggagt
      661 tttgcttctc aaaaacaaaa atatataaac gtaatccctt cgtatctgaa tatgttaaac
      721 gacttgcaaa aggtatttgt caactttgcc aagaaaaagg gccatttata aaagacggtg
      781 taccttattt acattgtcat catatagaat atttgtcgca aggtggaaaa gatgtgattg
      841 aaaactgtat cgcgttatgt cccaattgtc atgctagaat tcatgaactt gagttacctg
      901 acgataaaga aaaattgctg aagatcgttg aaaaaagaga aaatagcttt tctaaaatac
      961 cgtaatttat ttttatagga caaataagct aatgtgagct atgccttcta tataagacta
     1021 agttctatta taagtaacga agtaattctc gttccttcct gtagtaatat tttttaaata
     1081 acctataggc ttcttcaaaa gtacatcctc cagtaaattc taagtactgg aggattcttt
     1141 ttctagtgtt gctttactca tgaaaaagta gttggctact tcatcaaaat ttctgaactt
     1201 ttttaatgta taataaagag ctcgatcatt tactacaagg atgctagctc ctacattttc
     1261 acagtttttt agcaagtaaa tttttctaaa atcacatcag gaaatgcatg agattcttta
     1321 agagaggcaa ttatagggct atgattacgg ttcgtgtaag tgacgccaaa aaagaatggc
     1381 tttcttatat ggctgaattt tatgggatta ctttatctga acttctaaaa aactattcga
     1441 tggaacaact agaagatgaa tacaatcgtc aaacggctga aattgcccat aaacattgga
     1501 taagaagtgg gaaaacaaca gtattcatgg aagaatcctt aatgaatttg gtgggctgga
     1561 atgacctgcc gtttaaaatt cacgcccgaa attcagaagc agctctgatt ggcaaatacg
     1621 tgtattggta gagatagaag ataatcaatt cgttgttatc gctattcaag tagggcaccg
     1681 aagaaatgtc tatgattaag agactgttta tttttacaaa aaaataataa
//
LOCUS       DAZKNO010000266         1703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000266 DAZKNO010000000
VERSION     DAZKNO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.277"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..241
                     /locus_tag="ACG8MY_002677"
     CDS             <1..241
                     /locus_tag="ACG8MY_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0431805.1"
                     /translation="DFVGTTLSGYTEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTP
                     EQAKEIKKIGVAGIVVGGAITRPQEIASRFVKALS"
     gene            255..>1703
                     /locus_tag="ACG8MY_002678"
     CDS             255..>1703
                     /locus_tag="ACG8MY_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0431806.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGG"
BASE COUNT          512 a          236 c          363 g          592 t
ORIGIN      
        1 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
       61 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
      121 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
      181 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
      241 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
      301 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
      361 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
      421 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
      481 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatt
      541 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
      601 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
      661 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
      721 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
      781 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
      841 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
      901 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
      961 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     1021 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttggg ttaccagcag
     1081 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     1141 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     1201 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     1261 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     1321 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     1381 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     1441 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     1501 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     1561 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     1621 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     1681 gcatccaagc tgtttttgga ggg
//
LOCUS       DAZKNO010000267         1680 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000267 DAZKNO010000000
VERSION     DAZKNO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.278"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(67..1245)
                     /locus_tag="ACG8MY_002679"
                     /pseudo
     CDS             complement(67..1245)
                     /locus_tag="ACG8MY_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            1275..>1680
                     /locus_tag="ACG8MY_002680"
     CDS             1275..>1680
                     /locus_tag="ACG8MY_002680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431807.1"
                     /translation="MVTLFYRRDFLFSIYTKYFTLSSNIKTSTKLICKLFKLDKISNI
                     DDVKSKILTRKVSEPVKLTAQDKKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSG
                     EKVTFKINFITAISKLEYGKQKKRDNNDQYLPK"
BASE COUNT          511 a          300 c          323 g          546 t
ORIGIN      
        1 tgaagtaatt tggagggagc gtcaataatt ttgtgtaaat aactcgtcct tctgcaaaat
       61 aatgggttac tcagtaaaca ttgaagctaa tgtatccgtc acttgttgga agcctttatg
      121 gcttctattt aagaattttt gattgtatgt atcaaagatg gatactagga aacgctctag
      181 tgattcttca ttttgaaact gctcttttct gcggctgtat ttcttgattt gtttcttgaa
      241 agattcgatt aaattagttg agtaaatgct tcggcggata ccaggcggaa actcataaaa
      301 agtcaataaa tcttgatttt ttatcagtga ctgcgtcact ttaggataag ttttttgcta
      361 cttctcaatc atactcccta aaaaggtatt tgcctcttcc tttgagctag cttgataaac
      421 agccttaaaa tcatcacaga tttcttttcg atctttgaca cgtactttat gagcaatatt
      481 acgggaaacg tgaacacagc aatgttggta tttagcttta ggataaatct ggtgaatcgt
      541 atctttcatg ccttttaagc catcggtaat aaacagtaag acatcatgaa cccctctgga
      601 gtaaatgtcc tgtaacaact cattccaaac gtaggtcgat tcagtcggag caatcgcata
      661 acttagtact tctttagtgc cgtcttctcg tataccaatg gcaatataga ttgcttcttt
      721 ggagacggtt tgccgtttta aaggaatata agtagcatcc atgaaaatag cgacatactt
      781 atcatttaga gttctaactt taaatgcatt tacttcttca gtcagcactt tagtcatgtt
      841 ggacatggtt tggggcgtgt agtgatgccc atacattttc tcaatcaagt ctgcgatttc
      901 cgacatggta ataccttttc ggaagaggtg aatgacggtc tcctctaagg tatcattcgt
      961 ccgtctataa gcaggaacag tctgttgctt gaactcgccg ttgcggtcgc gcggaatctg
     1021 gagatgaagt tccccgtact cggtcttgac cgtacggtca taggagccgt tacgagaatt
     1081 ccccgtgtta aaaccaatgc gatcgtactt ttcgtaatcg aggaaagccg ttaactccgt
     1141 tttgagaagc gtattgacag ctttttccaa atggacacgg aataattcat tcaaatcgcc
     1201 tttgttcgct agagtcttta gaatttctgt agtaaaatcg ttcatatgga agtcctcttt
     1261 tctgtgaatg tgttgtggta actctattct acagaaggga cttccttttt tctatttaca
     1321 caaaatattt tacactctcg tcaaatataa aaactagtac gaaattaatt tgtaaattat
     1381 ttaagcttga taaaataagc aatatcgatg atgtaaagtc caagatatta acaagaaaag
     1441 taagcgaacc agtaaagcta acggcacaag ataaaaaaaa tggaattgtt ctttatggtg
     1501 atgatgcaaa gtataactat aattgggata cctcctcaga agctgaggta ttaagtattc
     1561 ctactaccga tacttcaggg gaaaaagtca cctttaaaat aaattttata acggcaattt
     1621 ctaagttgga gtatgggaag caaaaaaaga gagataacaa tgatcaatat ctacctaaaa
//
LOCUS       DAZKNO010000268         1656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000268 DAZKNO010000000
VERSION     DAZKNO010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.279"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..168)
                     /locus_tag="ACG8MY_002681"
     CDS             complement(<1..168)
                     /locus_tag="ACG8MY_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0431808.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAI"
     gene            503..937
                     /locus_tag="ACG8MY_002682"
     CDS             503..937
                     /locus_tag="ACG8MY_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0431809.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            960..1280
                     /locus_tag="ACG8MY_002683"
     CDS             960..1280
                     /locus_tag="ACG8MY_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431810.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1421..1612
                     /locus_tag="ACG8MY_002684"
     CDS             1421..1612
                     /locus_tag="ACG8MY_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431811.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
BASE COUNT          631 a          250 c          352 g          423 t
ORIGIN      
        1 aatggcattg atcaaggagt tatcaccaac gtatttttga gaaatcgttg atagagtatc
       61 acctgattga acggtataag cttcgtccgc atgagcagtc gttcccatga agaatgcagc
      121 gccggcagcc aaagttgttc caaaaagtaa agttttaagt gatgtcatga aaaatctcct
      181 ttattcgtta aaaagtttta tttgttgtgt tcaacgagga atactttaac acgtgaaaat
      241 atttcatagg taaaagaagc atgacaattt atgacaaatc cacgtttttt gtaacagaat
      301 gatgttcgct cgatgtgaaa aatgatgagg aatttcacat aatgcagaaa taatgggatt
      361 agaacaggtg ttctgatgag aaaagaaatg tataaataac ttgaaagaaa ttgcttttgt
      421 tcatcatttt atttacatat tgcgtatatt ttttgtattt ttgcttgaga tgttacaata
      481 aaagaaaaag gaggcggttc caatgagtaa aagataccag aacatcatgg tagcagttga
      541 cggatcaaga caatcgatca aagcgttcgc tgaagccctt gacttagcaa aggacaatga
      601 agcccactta tttatcgtgt caatcatcaa caaagttgag ctgacccata gtgcctatgc
      661 cttttcaaaa atctatgcag atgaaaaaca aagaacagaa gtagccatgc tgaaaaaaat
      721 caatgatgcc aaagagtacg gcattgctga gatccacgca atagttgaaa ctggagatcc
      781 aagaaatttg attgccaatg tcatccctgg acaagaaaat attgatttga tcgttatggg
      841 agcgacagga aaaggtgcta tacaacaagc attagttgga tcgaccgctt cttatgtcgt
      901 atcacatgcc ccttgcagtg tgttggtcgt gaagtaatga gataagggag gaatagaaaa
      961 tggttcgtcc tcaagaagtc cgtgcgccaa aagaaaaaat agaaatttta gcaattattg
     1021 aagacggcac acaaacaaag aagggatact cgatcgcttt agtaaagtgg aaaggaaaaa
     1081 aaggtgttgc tctgagatgg gatggagata accaacaaga taaaggattc cctgtcacag
     1141 ctaacggtta tcatccagcg tggtttgttc tgcctgataa atttatggaa ttatacagct
     1201 atgattatgc aagagccgca gcatcaatca aagcattaga taaattaaca gaagagcaga
     1261 atgaaatgga ggaaaaataa aaaagacgaa ccaatcagcc tttagaaaac tgattagttc
     1321 gaggagtaaa aatgaaaaaa ggttatttgg gatgaaacta gaataatgcg tataacttaa
     1381 ataaaactta aagcaataaa atggcaagag ggtgaggcaa atggaagcag gaaaagtatt
     1441 acaggaagta aaagcactat gtgcagataa aaaatatgaa gaagcgaaaa gcctgatcga
     1501 aaagaacaaa gaaaatttgg aagaaaagta cccgatcgca caagaattta tagaattaaa
     1561 acaaggaacg atccttcacc gattcaaaca tttttttggt ataacagaat agaaaaaacg
     1621 attaaagatc ggcagaaatt tgctgcatct ttaatc
//
LOCUS       DAZKNO010000269         1618 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000269 DAZKNO010000000
VERSION     DAZKNO010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.281"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            173..400
                     /locus_tag="ACG8MY_002685"
     CDS             173..400
                     /locus_tag="ACG8MY_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0431812.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            416..1582
                     /gene="hemW"
                     /locus_tag="ACG8MY_002686"
     CDS             416..1582
                     /gene="hemW"
                     /locus_tag="ACG8MY_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFQ0431813.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
BASE COUNT          549 a          255 c          353 g          461 t
ORIGIN      
        1 ttatgaataa aataaggaca aaattgttta ggaagaatca tgagtgtaaa aaaatcatcc
       61 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
      121 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
      181 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
      241 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
      301 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
      361 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
      421 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
      481 gcatatctgc tattactgtg actttaataa agttttttta gaagggcagc cagtagatga
      541 gtatgtggaa atgctgctga atgaaatgag attaatgctg gaaagtcatc ctacagcgaa
      601 tttggaaaca ctttatgtag gaggcggaac accaacttct ttatctgcta aacaacttga
      661 tcgactatta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
      721 agaagcgaat cctggtgatt tgacccgtga aaaacttcaa gtgatgaaaa actatggcgt
      781 caatcgttta tccatgggag tgcagacatt tgacaatcgc ttattgaaaa aaattggacg
      841 taaacatact gcagaagatg tgtatcaaac aatgcaattt ttagaagcag aaaattttac
      901 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatcggga
      961 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
     1021 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
     1081 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
     1141 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
     1201 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
     1261 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
     1321 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
     1381 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
     1441 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
     1501 tgaagaagac cgtgtttatt tgacaccaag agggactttt ttggggaaca atgtgtttga
     1561 gcgcttttta tttgaaaaat agtttgaaga atatttgttt tttatcgaaa cttgaaaa
//
LOCUS       DAZKNO010000270         1563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000270 DAZKNO010000000
VERSION     DAZKNO010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.282"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..686
                     /locus_tag="ACG8MY_002687"
     CDS             90..686
                     /locus_tag="ACG8MY_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0431814.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(744..1226)
                     /locus_tag="ACG8MY_002688"
     CDS             complement(744..1226)
                     /locus_tag="ACG8MY_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431815.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1332..1529)
                     /locus_tag="ACG8MY_002689"
     CDS             complement(1332..1529)
                     /locus_tag="ACG8MY_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431816.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          526 a          325 c          247 g          465 t
ORIGIN      
        1 tctgtttact tactttttgt aaggtgctat acttcatttc gtaatcaatt aaaaaaatgc
       61 aaaacgcaaa tcaaaggaga aattttatta tgacaacttt tactgacact ttgaaaaacc
      121 gccgttcaat ctatcatcta ggacgtaatg tttcactttc taatgaagaa ttaacaactt
      181 tgatcaaaga agcaattaaa gaaagcccaa ctgctttcaa tgctcaatca acacgcgcag
      241 ttatcctttt cggagatgca catgaaaaac tttgggaaat cactgaagaa gcacttcgcc
      301 cattgactcc agcagaagct ttcccaaata cacaaaacaa attagctggt ttcaaaaatg
      361 gttatggtac agtattattc ttcaaagata ctgacgtggt aaaaggtcta caagaacaat
      421 ttgaattata tgcagacaac ttcccagatt ggtcagaaca atcaaacgga atcgcaacag
      481 ctaatacttg ggtagcatta gttgataaag gattgggcgc aaacttgcaa cattacaacc
      541 cagttattga cgaagcagtt gcaaaagaat ggaatatccc ttcaaactgg aaactacgtt
      601 cgcaattagt attcggttca ccagaaacac cggctgggga aaaagaatac atgaacgatg
      661 ccgatcgctt ccgcgtattt ggataatttt atttgaaata aaaaacagag gttgtgacaa
      721 acatcttgtc gcaacctctg tttttattct ggatcaatca taatatcaat ctctgcctca
      781 tcgtttaagc gttgggacaa aaatggaact aattcttcta atgatgcaga aaatttcagc
      841 atcggtcctc gccatccgtt tcggaaaaaa ttcattttta cgattttaga accttttggc
      901 tgatataata caattttatt gatctcagaa aaaggaacac cttttttgtc aaagctattc
      961 aaaacaagtc cttcttctgt gattcctcta ctgtccaaca aaaaactaat aaaaatcatt
     1021 gctgacaaga tcccttttgc caattcatct gctgtaggat gactgatgag tgtataccaa
     1081 atgactagtg acactaagaa gggtatcacc aagtagaccc atcgatttcg cacagtccga
     1141 taaataattt cttttcgaac cagccaaaga aataaaaaca tcacaacaac gaataaaaac
     1201 aatagaaccg taaagaatgt taccatgaca actcgcctcg ctttttcttc tccagaaaat
     1261 aggaaaagaa tactgattta ccttctcaaa gaaattggac ttgcagcacg ccgcacgtcc
     1321 aattgttttc tttattcaaa tgtatcatat tttgcaacaa ccatgcttgc tttttcatct
     1381 aataattctt ggtcctctgc agcacattgg gcaactttga ccacggcgca gtttgtcatt
     1441 tttgatacaa cttcaccaat gaccgttttt gtaaaaccaa ttggtttgca tgcatattgt
     1501 tctcctaaat taatcatttc ttccgtcatc aaaatcctcc taaactgctg gaacttttct
     1561 cca
//
LOCUS       DAZKNO010000271         1561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000271 DAZKNO010000000
VERSION     DAZKNO010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.283"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..506
                     /locus_tag="ACG8MY_002690"
     CDS             132..506
                     /locus_tag="ACG8MY_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0431817.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="ACG8MY_002691"
     CDS             complement(620..1249)
                     /locus_tag="ACG8MY_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0431818.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="ACG8MY_002692"
     tRNA            complement(1383..1455)
                     /locus_tag="ACG8MY_002692"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
BASE COUNT          535 a          232 c          271 g          523 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 a
//
LOCUS       DAZKNO010000272         1552 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000272 DAZKNO010000000
VERSION     DAZKNO010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.284"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..185)
                     /locus_tag="ACG8MY_002693"
     CDS             complement(<1..185)
                     /locus_tag="ACG8MY_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431819.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLL"
     gene            complement(948..>1552)
                     /locus_tag="ACG8MY_002694"
     CDS             complement(948..>1552)
                     /locus_tag="ACG8MY_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431820.1"
                     /translation="VMQFTRQKLTAGKLFKSLFDLIIVNAILATLIKQIEQFGRTTKG
                     VKEFLMTFVHFVNGHMYLFEVGIILFLVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYY
                     VDFGRYDELINTDIVHTSIHEATVKGIRTTLKDGQETKAIDRRKEVYNFYEVEANLRY
                     GIGKLSLNGRNIPQNKPYIGWKSDALEVTKKLPMEEEEIQ"
BASE COUNT          471 a          281 c          220 g          580 t
ORIGIN      
        1 tctaacaacc gatcaaattg gtctttttta taattcagaa gagcgtgatc aaaatcattt
       61 tcgatcaaaa cttttaggta ttgatttcga ctccattttt tgccagtctt gaccgtaagt
      121 tcttcacaac gtttgtcaat tttattaatg tacttttctt caatatttct aattaataag
      181 tccatatttt cctccaaatt tattatgtgt agggatatgt gtagctacac atatccctac
      241 acattttttg attttgctac acacaaacgc ttattttact gcccacattc gctttcatcc
      301 tacccacaaa tctacacaca aatccaaaat tttgatatca tttatctttc ttagaaacgt
      361 ggtaggcaca gcctacctag cacctttcgt aagaaagtga tatcacgttt ccttatgctc
      421 tttaaaaatt aaggttattt gtgtatttag acaataaaaa atccttctaa ctttgtcgat
      481 tagaaggaaa tattttgtca tatgaaaaat gaatttaaga aaaagtgtat gctataattg
      541 atacgctgtc ttcctgtttg gttgaaatct tcagccagaa aggaggagtg ttaaatgttt
      601 tatgtgcttg atgtttgcgt tagaatcgtg gttaatgttt tctcccatct cattaacaaa
      661 tggttagatt ctaaacatga taaagacagc tagcctgttc attatttggt taatagtgaa
      721 taaagaaaag agccttacgc agctgcaact gctagggctc tttttgagta ttaaatgttt
      781 tatgtgtttg cattatcagt atagcataca cttttttcac agacaacttt ctacattttt
      841 tcttcttttt tcaatcaaaa taaactaatg aataatataa actcttattg agaactgttg
      901 gaaatgatta ctgatatctt caaacaaatg attcaaattg actagactca ttgaatctcc
      961 tcttcttcca taggaagctt tttagttact tctaaagcat cacttttcca gccaatgtat
     1021 ggtttgtttt gtggaatatt tcttccattt aacgacaatt ttccaatgcc atatcttaga
     1081 tttgcttcga cttcataaaa attgtagacc tcttttcttc ggtcgattgc tttcgtctcc
     1141 tggccatctt ttaacgtagt tctgatgccc ttaacggtag cttcatggat actcgtgtga
     1201 acaatatctg tattgatcaa ttcgtcatat cttccaaaat caacatagta ctcataacca
     1261 agaagtgtcg gaatcaagtc aatctcaatt tctttttcaa ttgaacgttt aataagtttc
     1321 gtggatacat acccgactaa aaagagaata atacccactt caaacaaata catgtgccca
     1381 tttacaaagt gaacaaacgt cattaaaaat tccttcactc cttttgttgt tcttccaaat
     1441 tgttcgattt gttttattaa agttgctaga attgcattta caataattaa atcgaataga
     1501 cttttgaaaa gttttcctgc tgttaatttt tgcctggtaa attgcatgac ta
//
LOCUS       DAZKNO010000273         1550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000273 DAZKNO010000000
VERSION     DAZKNO010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.285"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..350
                     /locus_tag="ACG8MY_002695"
                     /pseudo
     CDS             126..350
                     /locus_tag="ACG8MY_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            610..864
                     /locus_tag="ACG8MY_002696"
     CDS             610..864
                     /locus_tag="ACG8MY_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0431821.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            865..1182
                     /locus_tag="ACG8MY_002697"
     CDS             865..1182
                     /locus_tag="ACG8MY_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0431822.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          542 a          235 c          300 g          473 t
ORIGIN      
        1 agatacaata tcgatcagtt ttgaggaaat gaaattctag atattttttg ctaaatagag
       61 ttactttcaa tattttttaa cgtaattagg cagaagtcac tcatcctcta taggtttgtg
      121 atgaaattgc cattgatcca tttaagtaag agcaaaaaac tggaggttta tcaggtaatt
      181 ggtctaatgg atttaagtat gcggtcaccg tgtatagagt tgcttatgag attcaagaaa
      241 ataaagttat tccagtattg ctatatggaa cacataacaa tttttatgaa ttagccgaga
      301 acactcgcaa ctttattcgt gtagatgaat cggcttcggt tgagttataa ctttagctat
      361 atcaatcaac cgaccgacat atgttaaatc tatatataat aaaaatacct acttaaatgg
      421 atgttaacgg aagtgtatgg tgagtagttc actcgtattt tgatatcgtt tgtatatgtt
      481 ttcagcgttc taagtggctt attttgtcca ataataatat ggagtggaat cccttaagaa
      541 acaccgttgg caattgttgt tataacatgt tataatttat tcatcaaaaa ctaaaaggag
      601 tgtgataaca tgggaaaaga actaaaggtt agaaagatcg gcaatagcgt gggagtgatc
      661 cttccaagct ctcttggatt aaaaagcgga gacactatcc aagccaaaca agaaggcaac
      721 ttattcatat tagatactac tcaaatagca aaagaacacg atcgcaaatt gattgaagaa
      781 agtttccaag actttgagaa aggtttaaca gtttcagaaa ttgaaatggt aaaagctttc
      841 ggaaaatacg gctggagtga atagatgaaa gagtacacgc tagtttattc taaaagtttc
      901 cagaccagcc ttgaaacgat aatagaagaa tgggaaaatg agcttgctct gtctccagcc
      961 agcataaaaa gatttgtagg tgcgatccag aaggctttag aactaaccaa aaattttcca
     1021 actatgcatg aggaagtttc cgaaatctat ggaatggata tcccaacata tcggatcgtg
     1081 atcggcaaaa gctatgcctt attttatcgg atcgaccatg aaaaaaaaga gatacttgta
     1141 ggacgtattt tccaatctaa acaaatgaaa ctaggctttt aacaataatt aggcaaaagt
     1201 cgctcatcct ctacaggtgg gtgattaatg ccgctcgata tggttgttaa ccgtcggtta
     1261 ctcgaagatt gatatataag acttagtacc tttttatttt gctccatgtt atggccgaag
     1321 gtctaaagat acaatcatta gtctagagaa aaattaagaa ccgtaggaac tacggggatc
     1381 gcttggtgaa taagagacac tgctattagt gaaaaaataa gatatcaagt acgctctatt
     1441 cccaaaaatt tcgtgacttt actcatgaga ggttcaagta gtatattttt aatcaaaatt
     1501 gacgtaactt ttaataaata ctacctaaat aaggtagtat ttatttttgt
//
LOCUS       DAZKNO010000274         1529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000274 DAZKNO010000000
VERSION     DAZKNO010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.286"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1518)
                     /locus_tag="ACG8MY_002698"
     CDS             complement(52..1518)
                     /locus_tag="ACG8MY_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0431823.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          499 a          324 c          289 g          417 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctataccc
//
LOCUS       DAZKNO010000275         1518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000275 DAZKNO010000000
VERSION     DAZKNO010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.287"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..1259
                     /locus_tag="ACG8MY_002699"
     CDS             69..1259
                     /locus_tag="ACG8MY_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFQ0431824.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1393..>1518
                     /locus_tag="ACG8MY_002700"
     CDS             1393..>1518
                     /locus_tag="ACG8MY_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0431825.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTA"
BASE COUNT          486 a          299 c          316 g          417 t
ORIGIN      
        1 agaaaaaagg aagtcccttc tgtagaatag agttaccaca acacattcac agaagagagg
       61 acttccctat gaacgatttt actacagaaa ttctaaagac tctagcgaac aaaggcgatt
      121 tgaatgaatt attccgtgtc catttggaga aagcagtcaa tacgcttctc aaaacggagt
      181 taacggcttt cctagattac gaaaagtatg atcgcattgg ttttaacacg ggtaattctc
      241 gtaacggctc ctatgaccgt acggtcaaga ccgagtatgg ggaacttcat ctccagattc
      301 cgcgcgaccg caatggtgag ttcaagcaac agactgttcc tgcttataga cggacgaatg
      361 acacgttaga ggagaccgtc attcacctct tccgaaaagg tattaccatg tcggaaatcg
      421 cagacttgat tgagaaaatg tatgggcatt actacacgcc ccaaaccatg tccaatataa
      481 caaaatcatt tacagaagag gtaacggcgt ttaatgggcg ggagcttcat gaccgttatg
      541 ctgctattta tatggacgca acgtatattc cgttaaagcg gaaaaccgtc gccaaggaag
      601 ctattcatat cgcagttggc attcgcccgg acggatcaaa ggaagtattg agctatgcga
      661 ttgcaccgac tgaatccatc acgatttggg aggaaatttt attggacctt caagagcgcg
      721 gtttgaaaaa tgtcctcctg ttcatcacgg atggcttaaa ggggatggta ggagcgatta
      781 gtcggttcta tcccaaagct cgttttcaac attgttgtgt acacgtttcc cgtaatatca
      841 gtcataaagt gcgtgtcgat gatcgtaagg aagtctgtga tgattttaaa atggtgtatc
      901 aagcctcatc taaagaggtg gcgttggaag cacgtggtac ttttgcggaa aaatggaaaa
      961 ccagctatcc aaaagtggtt gaatcgattc tttcgaacga tcacttgctc actttctatg
     1021 atttcccctt ggccatacgc aagagtattt attctacgaa cttgattgaa tcctttaata
     1081 aacaaatcaa gaaatacagc caccgcaagg aacagttcca aaatgaagag tcgatggaac
     1141 gtttcttagt ctcgtctttt gatacttaca accaaaaatt cctaggtcgc agtcataaag
     1201 gtttccaaca ggccgaaggc gagctcgaac aaatgctaag ccaactgatt gagaattaga
     1261 aacaatctgc agtagggaag aatcatttac acaaaattat tgacgctccc ttataatgag
     1321 aacttttact aacataattt atgataaact tactatataa tcatattaat taaatagtta
     1381 ggagatctgc ttatgtttat atctactctt caaattcgaa attttagaaa ttatgaatcc
     1441 gaaatctttc gattcgataa tgagacaaat actattatag gtgagaatga ttcagggaaa
     1501 accaatgctc ttactgca
//
LOCUS       DAZKNO010000276         1490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000276 DAZKNO010000000
VERSION     DAZKNO010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.289"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..905
                     /gene="scrK"
                     /locus_tag="ACG8MY_002701"
     CDS             33..905
                     /gene="scrK"
                     /locus_tag="ACG8MY_002701"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0431826.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(1139..1490)
                     /locus_tag="ACG8MY_002702"
                     /pseudo
     CDS             complement(1139..1490)
                     /locus_tag="ACG8MY_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          441 a          282 c          330 g          437 t
ORIGIN      
        1 gatttcattc taaaataaag ggagaaatgt ctatgctata cggtgcaatt gaagcaggcg
       61 gcacaaaatt tgtgtgtgcc attggcgatg aagaaatgac gatcaaggaa agagtgagct
      121 ttccgacaac gacacctgag gaaacaatgc ctttagtcat tgacttcttc aaacaatacc
      181 aagcagattt agcaggaatt gggattggct catttgggcc tatcgatatc catcgcgatt
      241 ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat tttgatttca
      301 tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat gtcaatgcgg
      361 ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc gtttactata
      421 cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc gaagggttta
      481 gtcatccaga aatggggcat atgctcgtcg tccctcatcc aaaagatgac tttgctggca
      541 gctgtccatt tcatggcaac tgtttagaag ggatggccgc tggacccgcc atcgaaaaac
      601 gcttagggaa aaaaggacaa gaggttgcag aagatgatcc ttattgggaa atcgaagcct
      661 cctatattgc tcaatgtgcc tacaatacaa ccttgatgtt ttctccagat gtgatcattt
      721 ttggcggtgg tgtgatgaaa caacgccata tgctgcaaaa cgtccacgac gcatttgcaa
      781 aattggtcaa tgggtatgtc gaaacacctg agttaagcaa atatatcgtg accccagcat
      841 tagaagacaa cgctggaacg ctcggttgtt tggcattggc tcgagaagcg gtcttacatt
      901 cataaacgaa aaaaggctct tcgacattta gtgttggtag agaaagatac aactgaaaat
      961 ttagtcataa ccagttaggc agagtgctta actggttttt gttttacttg gaagatcaag
     1021 ccttgaatga gataaattct tgctcgaaag ttatggaaat ttcgatagcc gtaggctact
     1081 tgtattgtgc aatagttttt tacaaaaaaa aggggtgcgg atattttttt ggaccttttt
     1141 aacaaatata tctttgcttc ttccgatatt gaagagggct tagccctcct aaagataact
     1201 taattcgttg attattatac caatgaatat aattatccaa tttgtttata aactgattga
     1261 ttgttgtatt cttccagtca cgttgataaa acatctcgtt ttttaatcgt ccaaagaaac
     1321 cttcgcacgc tgaattatct ggtgaataac cctttttaga catagagtga agcaagttgt
     1381 ctttgttcca atagtccaac taacaatagc accatcgtaa caatcgacaa gtggtgataa
     1441 gtagattttc cctgcgggaa ttctaaattc agtaatatct gttaaccatt
//
LOCUS       DAZKNO010000277         1447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000277 DAZKNO010000000
VERSION     DAZKNO010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.290"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8MY_002703"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG8MY_002703"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0431827.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..1406
                     /locus_tag="ACG8MY_002704"
     CDS             1131..1406
                     /locus_tag="ACG8MY_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431828.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT          451 a          285 c          215 g          496 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1321 aaaaagaatt tcataaacag atgaaaaaac tgattgaaaa agaatttcca agaagtcata
     1381 aactctatgt gaatcagcaa ttttaacttc taactgttct ttattaatta aaaatgttct
     1441 gtaataa
//
LOCUS       DAZKNO010000278         1362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000278 DAZKNO010000000
VERSION     DAZKNO010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.291"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1359
                     /locus_tag="ACG8MY_002705"
     CDS             <1..1359
                     /locus_tag="ACG8MY_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0431829.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          455 a          244 c          290 g          373 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aa
//
LOCUS       DAZKNO010000279         1361 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000279 DAZKNO010000000
VERSION     DAZKNO010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.292"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..462)
                     /locus_tag="ACG8MY_002706"
     CDS             complement(<1..462)
                     /locus_tag="ACG8MY_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431830.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRE"
     gene            complement(702..>1361)
                     /locus_tag="ACG8MY_002707"
     CDS             complement(702..>1361)
                     /locus_tag="ACG8MY_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0431831.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          407 a          246 c          195 g          513 t
ORIGIN      
        1 ttccctgtaa ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc
       61 acccattcca ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc
      121 agtttgttta tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc
      181 aaaaattttt tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac
      241 tgataattga tactctatat tacatgactt aattagtttt ttagatcccc cctccagata
      301 ggcatatgtt tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc
      361 ataaattaaa aatgactgtc tatttagccc ataatctaat acaacttctt catactcata
      421 tttctttaaa tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt
      481 gttatttatt gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt
      541 agtgaactac tcaccactta gctaaaccta acggtttctt acgcttgaag tgggagcttc
      601 ttgggaagag agcatacttg atagcgtatt actttactaa cagaggtgcc tcttatttac
      661 caagctatcc ccgtagttcc tacggttctt gtttatttga tctaagccaa tcccatagag
      721 tgtagcctta gaccttcggt caggatattg atactagcgt tgatatctcg gtcatgatga
      781 gtatgacaaa tagggcaagt ccattctcga atttcgagag atttcttgcc gtctttatgt
      841 ccacaattcg aacagatttg gctagatgga aaccacttat ctactttgat gattttccga
      901 ccgtaccagt cagccttgta ttgtaattta gacacaaaac tagaccaaga aacatccgaa
      961 atactttttg ctagtttatg attacgcaac atgccttttg tgtttaagtc ttcaatacag
     1021 atgatatcgt gatttttgat aatttctgta ctcaacttat tcagaaaatc agtacgttga
     1081 ttcattactt tttcatgcaa tcgtgctact ttacgttttt gtgtttgata atttctagct
     1141 tcaaacagat tgataccttt ttgtttagct aataaggcac gtcttgacaa cttacgttgt
     1201 tcacgtttga gtttcttttc cattttggac gtaaatttat tattatcaaa tttttgaccg
     1261 gtagaaagaa ttgcaaagtc tataatacct aggtcaatac caacagcaga attagattta
     1321 ggtagttcgc gaacttcttc tttgcataac aaagaaatat a
//
LOCUS       DAZKNO010000280         1334 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000280 DAZKNO010000000
VERSION     DAZKNO010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.293"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(57..263)
                     /locus_tag="ACG8MY_002708"
                     /pseudo
     CDS             complement(57..263)
                     /locus_tag="ACG8MY_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            412..>1334
                     /locus_tag="ACG8MY_002709"
     CDS             412..>1334
                     /locus_tag="ACG8MY_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431832.1"
                     /translation="MKRFLKIFIFLEIILFVYIFNTSIYNIYEKNNIATDNLKGYVLE
                     ETSPEILDKFYTIFTEEYSQNKLELINNKLTSTDKSVYDLYCYPLNEFVQKQPISNSI
                     LLQYHKLQKEDFLDSVGVFYTDLQANEIREIESQLAVTISVFENEAIPYSMILELYLL
                     NFVILFVVLQIIYCIYTSDSLKKKGIKKSMGFSAMHILKEQINNIVKYFIVICSVLFI
                     LLNLYYALTNRFNISYLTISIFFFAIILVINILCVLITSFLIKFVSLEAMIKNKTLNS
                     GTNLIVQTIKIVFSVIITITTISLLNQGNEF"
BASE COUNT          452 a          172 c          213 g          497 t
ORIGIN      
        1 attctattct ataggtcggg ttatttgacg tacaggtact gttctatggg gcgcttactt
       61 tgaatggccc tttttacaag ctgcgcatct tttttatttc cgcaagaatg gccaataatt
      121 tcacgattga ataaatcaat catcaaacaa atataagccc aatttgtccc gacccgaaca
      181 taagttaaat ccgtgacaac cgctgacaat gcttttgttt gagtaaactt tcgatctaag
      241 tgattcgcaa ttttcttttg attgctttgg cacgatcata aagacgggag cagatattgt
      301 ctattgatat aatgatagta gacaaacaga gttggctgat tagctaaagc atatcgagta
      361 taatttgaat attagacgca tgatttatca gtgtaagaaa gggtgaggat tatgaaaagg
      421 tttttgaaaa tatttatctt tctcgaaata atactttttg tatatatttt taatacttct
      481 atttacaata tttatgaaaa gaataatatt gcgactgata atttaaaagg atatgtttta
      541 gaggaaacat cgccggaaat tcttgataaa ttttatacga tatttacaga agaatattcc
      601 caaaataaat tagagcttat caataataaa cttacttcta cggataaatc tgtctatgat
      661 ttatattgtt atccgctaaa tgaatttgtg caaaaacagc ctatatcaaa ttctattctg
      721 ttgcagtatc ataaattgca aaaggaagat tttttggata gtgtcggggt attctatacg
      781 gatttgcaag ccaatgaaat acgtgaaata gaatctcaac tggctgtaac aattagtgtt
      841 tttgaaaatg aagctatccc gtatagtatg atattagagt tgtatcttct taattttgta
      901 attttgtttg ttgtacttca aataatatat tgtatttata catctgatag tttgaaaaaa
      961 aaaggtatta aaaaaagtat gggcttttct gctatgcata ttttaaagga gcaaatcaac
     1021 aatatagtaa aatattttat tgtgatatgt tcggtgttat ttatcctgct gaatttgtat
     1081 tacgcattaa caaatcgttt taatatttct tatttaacaa taagtatatt tttctttgcg
     1141 attattttgg ttataaacat actttgcgtt ttaattacca gctttttaat taagtttgtt
     1201 tctttggagg caatgattaa aaataagact ttaaatagcg gtacaaatct tatagttcag
     1261 actataaaaa tagttttttc agtaataatc acaattacca ctatttcatt attgaatcaa
     1321 ggaaatgaat ttaa
//
LOCUS       DAZKNO010000281         1324 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000281 DAZKNO010000000
VERSION     DAZKNO010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.295"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..820
                     /locus_tag="ACG8MY_002710"
     CDS             278..820
                     /locus_tag="ACG8MY_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431833.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..>1324
                     /locus_tag="ACG8MY_002711"
     CDS             976..>1324
                     /locus_tag="ACG8MY_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFQ0431834.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          533 a          157 c          248 g          386 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccag
//
LOCUS       DAZKNO010000282         1295 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000282 DAZKNO010000000
VERSION     DAZKNO010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.297"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1024
                     /locus_tag="ACG8MY_002712"
     CDS             <1..1024
                     /locus_tag="ACG8MY_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HFQ0431835.1"
                     /translation="IFRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKNLA
                     QMIQERIKEELGLIATVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPE
                     MTDFWGIGSRMKKRLNQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIA
                     IPPEPTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGT
                     SILETRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQ
                     LDLFSNPQKQIDDEKLDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGA
                     GELKND"
     gene            1017..>1295
                     /locus_tag="ACG8MY_002713"
     CDS             1017..>1295
                     /locus_tag="ACG8MY_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431836.1"
                     /translation="MIDYNDRGRLKWVGFYLSDHTEEIDADNEQRANNNLAKEQMTTE
                     EISEVLNAAVIKNRSVCIQKEERNAEGYYPDDIVGKILGSDELGIYVGK"
BASE COUNT          456 a          207 c          302 g          330 t
ORIGIN      
        1 tattttcaga agatatgtgg ctgatgaaga tctactgatt tactcgatcg atgaatcaat
       61 cttaaaagtg acccgatcac tgaatctttt tacgactgaa ggaacacgaa gtcagcgaag
      121 gaagaatctc gctcaaatga tccaagaacg tattaaagaa gagctaggat tgattgctac
      181 ggtaggcgtc ggcgataatc cccttttggc taaattagcc ttagacaatg aagcaaagca
      241 taatgaagga tttattgcgg aatggacgta cgaaaacgtt cctgagaaag tatggaatat
      301 tcccgaaatg acggactttt ggggaattgg ttcgcgaatg aaaaaaaggc tcaaccaaat
      361 gggaattttg agcattagag accttgctaa ctggaatcct tatacgatta agaatagatt
      421 aggtgtgatt ggattacagc tgtacttcca tgctaacgga atcgatcgaa cagatatagc
      481 aatcccgcca gaaccgacaa aggaaaaatc ttatggtaat tcacaagtgt taccacgaga
      541 ttatactaga agaaatgaaa tcgagctggt ggtaaaagaa atggcggaac aagtagccat
      601 tcgtatcagg cagcataatt gcaaaacagg atgtgtccat ttaaacattg gtacgtcgat
      661 cttagaaacg agaccaggat tttcacacca gatgaaaatc cctatcacag ataatacaaa
      721 ggaattgcag aattattgtc ttttcttgtt tgataagtat tatgaaggac aagaagtaag
      781 gcacgttgga ataacctata gcaagctagt ttatactgat tcgcttcagc tggacttgtt
      841 tagtaatcca caaaaacaga ttgatgacga aaaactagac aaaataatag ataaaattag
      901 gcagaagtat ggttttacgt caattgtaca tgcttcttcg atgttagaag gcgctcgtag
      961 tattactaga agcacgttgg ttggtggcca tgcaggcggc gcaggggagc ttaaaaatga
     1021 ttgactataa cgatcgagga cgattgaaat gggtaggatt ctatttgtcc gatcacacgg
     1081 aagagatcga tgcggacaat gagcaacgtg caaataataa tttggcaaaa gaacaaatga
     1141 cgactgaaga gatttcggaa gtattgaacg ctgcagtcat aaaaaataga tcagtatgta
     1201 ttcaaaaaga agaacgaaac gctgaaggat attatccaga cgatattgtt ggaaagatct
     1261 taggtagtga tgagctgggg atctacgttg gcaaa
//
LOCUS       DAZKNO010000283         1249 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000283 DAZKNO010000000
VERSION     DAZKNO010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.299"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1051
                     /locus_tag="ACG8MY_002714"
     CDS             <1..1051
                     /locus_tag="ACG8MY_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0431837.1"
                     /translation="DISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFVSTKDDLWIYV
                     FITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLFMQVYTTLDKT
                     IVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKSENILVYTKKS
                     FDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVSPIGGVFANQF
                     AIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLVFFFRVFFVRK
                     EIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMIFIIYLPFVYF
                     LFPDIRNEIRKITRK"
BASE COUNT          395 a          167 c          210 g          477 t
ORIGIN      
        1 agatatttct tggttttttc aaggaattga agaattttcg aaagtggtta ttagaaatac
       61 agttatcaaa ttatcatcgg tattgttaat aatcattttt gtttcaacta aagatgattt
      121 gtggatttac gtgttcatta cttcaattac taacttttta ggaagtttag ttttctggtt
      181 aagtatccac ggaaaagtag gtaaaccaat ctttaactct aatgttttta aacagttgtg
      241 gaaaccagct tttattattt tgactcctca gttgtttatg caagtgtaca caactttaga
      301 caaaacgata gttgggtatt ttgtggatcc aacggaatta tcttattatg atcagtctca
      361 aaaaattgct cgtattatat tggcagtctt aacatcaatt actattgtta tgttgcctaa
      421 aatatctaaa gcacaggcag aggggaaatc agaaaatatt ttagtttaca caaaaaaatc
      481 ttttgattac acgattatca ttgcattaat tctttttagt gtggtgttta tcaatacaaa
      541 agaatttgtt ccatggtttt ttggggagaa tttcaaaccg atgacaataa acatgcttat
      601 ttcttgtcta attatagttg ttagtccaat tggtggagtt tttgctaacc aatttgcaat
      661 tgctatagaa aaagataagg agtatgctta tccaatgatc attggtgcga ttgtaagtat
      721 tattggaaat gtagcttttg tgcctactta tggcgctgta gcggctacga tcgtactcgt
      781 ggtagtagaa ttattagtct ttttctttag agtatttttt gtacgaaaag aaataaaaat
      841 atcctatttc tttgggaaga acgttatctt attttttata gtcaatctta ctttgactac
      901 ttgggcctat tactttcttc cacaattatt ctcttcttca tttttagata tggcaattaa
      961 atcaataatg attttcatta tttatcttcc gtttgtttat tttctttttc cagatatacg
     1021 aaatgaaata agaaagatta ctcgaaaata aaataatgtt gtaaaaaagt agttctttgt
     1081 caaatcgggc tgataaggtc atttatgaac aacaaagtta gtaagagtta ctagctttta
     1141 atgcatagct gattaacctg ttgattagaa aaatccacaa gcgcatattg gtaaaactgc
     1201 gaaacccata agcaaatcgt ttcaatgttt tgatatgtgt gcgaagatt
//
LOCUS       DAZKNO010000284         1230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000284 DAZKNO010000000
VERSION     DAZKNO010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.300"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACG8MY_002715"
     CDS             <1..704
                     /locus_tag="ACG8MY_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0431838.1"
                     /translation="IKKNTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPT
                     SVKYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLEN
                     MFLRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKII
                     AITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVE
                     LREKVLRSIIDKSIE"
     gene            934..1164
                     /locus_tag="ACG8MY_002716"
     CDS             934..1164
                     /locus_tag="ACG8MY_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0431839.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          436 a          180 c          204 g          410 t
ORIGIN      
        1 atattaagaa aaacacaaaa gttggcgatg ctactattat tcgcttttgt caaaaaattg
       61 gtttttctgg tttttctgat ttaaaaattg aaattgcaaa ggaagacttt agtcaaaaaa
      121 aagaacatcc gactagtgta aagtactatg atgaaattgc taatagttta acagaagctc
      181 tctattccac tattaggctt ttagacgaag aaaaattgaa tgaagccatt aaaatgatca
      241 ctcaatcaaa aaacatatat atatttggag tgggatctag tggaaatact agtcttgatt
      301 tagaaaatat gtttcttcgc gtaggagttc aagcaaaagc tgtactagac cctcattttc
      361 aagcccaagt cgcttcttta ttaacagtta atgatttagt aatcattttt tctttatctg
      421 ggaaaacgaa agatacttat gattcattga aaatagctaa aaaaaatggc gcaaaaatta
      481 ttgctataac taactatatt cattcaccta taggaaagtc tgcagactta gttttacaaa
      541 cagccattga agaatttctt aatggtggat ctttagctgg aaaaatatca caactttata
      601 tttgtgatct attagttcat ggttatgaac aaaacaataa tattgattca gttgaattaa
      661 gagaaaaagt tttgcgctct attattgata aaagtattga gtgaactacc cccacttagc
      721 taaacctaac ggtttttaac gattgaagta ggcaattctt gggatgagag catacttgat
      781 agcttatttt ttactaatag cggtggctct tattgaccaa atatccctgt agttcctacg
      841 gttcttgatt ttatctaaag atatttttat cgtggaagct attgatcaac ttgaaagaaa
      901 aacgtgtagc tcattatcac tagtttatcg agtatggatg aagcaatgga tcatcctctc
      961 ttaaataaat ttattaagga tctgatcctt caaatttttt ccatgatcga tgagcaggaa
     1021 cctactgaat ctaaacgaca tcgggcacaa ggaattatta agattgctaa gcgaatgttt
     1081 tctataaagg gtgttctaag ctatacagtg ttaattcaaa agattcccaa tcatcttgtt
     1141 tataagaaca acgttgaaga ttaaaaaagt gtttcaatgt taaaattgca aaatacttca
     1201 atgttatgag acaaactgtc tataagaatc
//
LOCUS       DAZKNO010000285         1211 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000285 DAZKNO010000000
VERSION     DAZKNO010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.301"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..369
                     /locus_tag="ACG8MY_002717"
     CDS             <1..369
                     /locus_tag="ACG8MY_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0431840.1"
                     /translation="IETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAI
                     RSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFAS
                     LKNKALELIELQISEGIDKQ"
     gene            445..663
                     /locus_tag="ACG8MY_002718"
     CDS             445..663
                     /locus_tag="ACG8MY_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431841.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            650..>1211
                     /locus_tag="ACG8MY_002719"
     CDS             650..>1211
                     /locus_tag="ACG8MY_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431842.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNS"
BASE COUNT          445 a          157 c          225 g          384 t
ORIGIN      
        1 atcgaaacat ttattgaaga aaatcttctt aaacaaatat ggggagaaag tattgtgaat
       61 tgcttaaaac tggctagcaa tagtgattat cgtcaattcg acaactggta caaaaaattc
      121 aaatatgcga ttagatctgc tgaaaaagaa caaaaaattc aactgaaaat tatctatgaa
      181 atttgtaata acaagtattt tgtagatcat gttagagaac agctatcaat gactttacgt
      241 gacttgatac gaagagcaaa aacagatcat cgtatcaaac aaaaagacga ttatatcttt
      301 gcttctctaa aaaataaagc gttagaacta attgaattgc aaatcagcga aggaatagat
      361 aagcaataag aatagaattg agtcaaatga aatggagggg tatctccact tcatttgaga
      421 actttgaata taggaggggt tattttgatt aaaaatcgtt taatagatca agtttggtgt
      481 ctagatcaaa atacagatag tctcgaatta atcgagacta tttgcttcaa cactatagtt
      541 ctcgatttaa gagctgaaaa tgataattgt gtaactcatg tcataattaa tcaaaaaatg
      601 gctcagaagc tctctgagag cctcagaaaa tgggctaagg gaggtaacga tgaaaataat
      661 tgaaaaaaag attgccaata aaaaaataaa ttggttggtt gttaattttc ttgttctgat
      721 agctatgatt atttttacaa gtaatattgc tcatgcgagt attttcaatt ggtttgatga
      781 cacaaaaagt caaacagagt ttttgaataa ttctctttat agaccttacc taaaaaagta
      841 caatggagat ttccttagtc agttcgggat ttggctcgga tgggctgtgg taaaaggcat
      901 gtttactgtg acagattcga ttcaaaatat gattccagat gtgctggatt tgttcaattt
      961 cattgaaagt acagggctaa ataatgttta tcaatcagcc gtaaatacaa ttgtcattgg
     1021 attaatgatc ctttcattga tgtttgttgg ttacaaaatg attactggaa aaggaacagt
     1081 ggatttaaaa tcggttggaa tgaacgtagt gatgtctata gcattaatac ttttaatgcc
     1141 aaccatgatt tctagtggta ttcaatttgc taaaacattt tataaggact ctactacgat
     1201 tacaaattca g
//
LOCUS       DAZKNO010000286         1210 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000286 DAZKNO010000000
VERSION     DAZKNO010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.302"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            311..>1210
                     /locus_tag="ACG8MY_002720"
     CDS             311..>1210
                     /locus_tag="ACG8MY_002720"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0431843.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTA"
BASE COUNT          471 a          187 c          244 g          308 t
ORIGIN      
        1 aaaagtgtct gatttgaata tcccagcagc ggaagaagaa aaacaaaatc gataacattt
       61 gttctaagaa ttagttacgt taaatacgga acgcaagata gacttgaaaa acggtaacaa
      121 aagaagtagt atattaaggt gtgatgtgct acttgacatt tattctgctg agcgaacttt
      181 ctggattgtg tagccttata tctattcagg acgtatcgag cagattagta aaggtgttct
      241 tgatgatttt catcttgaac acctttgttt ttagcaattc gcaaaaaacg aaaagtagga
      301 gaattgataa atgaaaaaaa gatttttagc acttgccatc gtactaggca ctggcttatt
      361 gtcaggctgt acgaatgccg gtgaaaaaac agccgttagt tataaaggcg gtacaatcag
      421 cgaacaagaa gtaatggata gcttgaaaaa aatgcaagga gcagattccg ctgtgcaaca
      481 gttgatcgtt tatcaagtat ttgaagataa atatggagat gacgtatcaa ccaaagaaat
      541 cgattctcaa tatgatcaaa caaagaaaca attgggtgat tcatttgatt cacaattgaa
      601 atctgcaggt tacacagaac aaacattcaa agacagtatc aaacaaagtc tagcctttca
      661 agaaggtcta aagaaacata tcaaattgac cgatgaagat ttaaaaacgg cttgggaatc
      721 tttccatcca gaagtggaag ctcaaattat ccaagtagca agtgaagatg atgcaaaaga
      781 tgtgaagaaa gctgctgata agggagacga tttctctaaa ttagccaaag acaagtcaac
      841 tgatactacg acaaaagaag atggcggaaa agtgaaattt gattccacta ctacaacggt
      901 tccggcagaa gtcaaagaag cagcctttaa gttgaaagat ggtcaagttt ctgatgtgat
      961 cacttctacc aatgcttcaa cttatactac ggaatattat gtggttaaaa tggtgaaaaa
     1021 ccaaaacaaa ggcaacgaca tggataaata caaaaaagaa ttgaaagaaa ttgcaacaga
     1081 cacaaaatta agtgacagta ccttccagaa taaagtaatt ggcgaagtat tgaaagacgc
     1141 taatgtaaaa atcaaagaca aagactttga aaatgttttg agtacattca cttcagattc
     1201 tagtacagca
//
LOCUS       DAZKNO010000287         1188 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000287 DAZKNO010000000
VERSION     DAZKNO010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.303"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            56..>1188
                     /locus_tag="ACG8MY_002721"
     CDS             56..>1188
                     /locus_tag="ACG8MY_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0431844.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQY"
BASE COUNT          424 a          183 c          246 g          335 t
ORIGIN      
        1 tatttatcac tgggattttt atgccctttt gggtttttga atggaggaaa atcacatgaa
       61 aattattaat attggagttt tagctcatgt tgatgcagga aaaactacct taacagaaag
      121 cttattatat aacagtggag cgattacaga attaggaagc gtggacaaag gtacaacgag
      181 gacggataat acgcttttag aacgtcagag aggaattaca attcagacag gaataacctc
      241 ttttcagtgg gaaaatacga aggtgaacat catagacacg ccaggacata tggatttctt
      301 agcagaagta tatcgttcat tatcagtttt agatggggca attctactga tttctgcaaa
      361 agatggcgta caagcacaaa ctcgtatatt atttcatgca cttaggaaaa tggggattcc
      421 cacaatcttt tttatcaata agattgacca aaatggaatt gatttatcaa cggtttatca
      481 ggatattaaa gagaaacttt ctgccgaaat tgtaatcaaa cagaaggtag aactgtatcc
      541 taatatgtgt gtgacgaact ttaccgaatc tgaacaatgg gatacggtaa tagagggaaa
      601 cgatgacctt ttagagaaat atatgtccgg taaatcatta gaagcattgg aactcgaaca
      661 agaggaaagc ataagatttc agaattgctc cttgtaccct gtttatcatg gaagcgcaaa
      721 aagcaacata gggattgagc agcttataga agtgataacg aataaatttt attcatcaac
      781 atacagaaag aagtctgaac tttgcggaaa tgtcttcaaa attgaatatt cggaagaaag
      841 acaacgtctt gcatatgtac gcctttatgg cggaatcctg catttgcggg attcggttag
      901 aatatcggaa aaggaaaaaa taaaaattac agaaatgtat acttcaataa atggtgaatt
      961 atgtaaaatt gataaggctt attccgggga aattgttatt ttgcaaaatg agtttttgaa
     1021 gctaaatagt gttcttggag atacaaagct attgccacag agagagagaa ttgaaaatcc
     1081 gctccctctg ctgcaaacaa ctgttgaacc gagcaaacct caacaaaggg aaatgttact
     1141 tgatgcactt ttagaaatct ccgacagtga cccgcttcta caatatta
//
LOCUS       DAZKNO010000288         1180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000288 DAZKNO010000000
VERSION     DAZKNO010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.304"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..>1180
                     /locus_tag="ACG8MY_002722"
     CDS             36..>1180
                     /locus_tag="ACG8MY_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0431845.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGIT"
BASE COUNT          389 a          234 c          232 g          325 t
ORIGIN      
        1 ccacaaaata aacaactgag aggacgatac gctatatgaa tgattctatc aaaaaaatgc
       61 tgagaataac agagaaagat ttgatgatta cagaggtctc ttacgagacc cttcagaaga
      121 aaaagacgtt ggtcgtcgat gctgttctct cgcctactcc tcgtgcttgt agaagttgtg
      181 gttctactgt ggtagatgga aacgggaaag caattatagt gaaaaatgga aaaaaggaaa
      241 cgattgtccg ttttgaacaa tacaatcata tgcctttggt tatgcgccta aaaaagcaac
      301 gctatacctg taaaaactgt cgcacccatt ggacggctca aagttatttt gtccaaccca
      361 gacattcaat cgcaaatcat gttagatata aaattgcttc tttactgact gaaaaagtat
      421 ctttatcttt tattgcgaaa agctgtcagg tatctttgac caccgttatt cgtacattga
      481 aagagtttaa aagctattta ccaaagcaat ctaagaagat cctccccaga gtattgatgg
      541 ttgacgaatt tcgttcgcat gcttccatag aagataagat gagctttatt tgcgcggatg
      601 gcgaaacagg aaaattaata gatgttttgc ctacgcgtaa attacctcga ttaacaagct
      661 atttcttagg ttgtaccaat ccagaagaag tagaattctt ggtgacagac atgaacgccg
      721 cctacttcca gctcaccaaa cgtgttctgc caaatgcgaa agtcgtgatt gatcggtttc
      781 atattgtcaa acacatgaat caagcgttca atgagttgcg tatccgtgaa atgaatgaac
      841 ttcgtaaagc cggacagaaa agccaggcag aaaaactgaa aaagaactgg cgctttttgc
      901 taaaaaatcg tgcaaacatc aaccattatg aatacaaaac atggaaaagt ttccgagcac
      961 caaaataccc atttcttact gaagcaatga tgattgatcg attgcttgag ttttctccgc
     1021 ccctaaagga ggcgtatccc ttttttcatg aattagttga agcctttcga gacaaagacc
     1081 ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac agctttcggg
     1141 aaaagcttca aaaccttctg acctatgaag aaggcatcac
//
LOCUS       DAZKNO010000289         1144 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000289 DAZKNO010000000
VERSION     DAZKNO010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.305"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..111)
                     /locus_tag="ACG8MY_002723"
     tRNA            complement(27..111)
                     /locus_tag="ACG8MY_002723"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75..77),aa:Leu,seq:caa)
     gene            complement(132..202)
                     /locus_tag="ACG8MY_002724"
     tRNA            complement(132..202)
                     /locus_tag="ACG8MY_002724"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Cys,seq:gca)
     gene            complement(248..319)
                     /locus_tag="ACG8MY_002725"
     tRNA            complement(248..319)
                     /locus_tag="ACG8MY_002725"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:Gln,seq:ttg)
     gene            complement(331..403)
                     /locus_tag="ACG8MY_002726"
     tRNA            complement(331..403)
                     /locus_tag="ACG8MY_002726"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(368..370),aa:His,seq:gtg)
     gene            complement(406..479)
                     /locus_tag="ACG8MY_002727"
     tRNA            complement(406..479)
                     /locus_tag="ACG8MY_002727"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Trp,seq:cca)
     gene            complement(486..566)
                     /locus_tag="ACG8MY_002728"
     tRNA            complement(486..566)
                     /locus_tag="ACG8MY_002728"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(530..532),aa:Tyr,seq:gta)
     gene            complement(568..643)
                     /locus_tag="ACG8MY_002729"
     tRNA            complement(568..643)
                     /locus_tag="ACG8MY_002729"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(608..610),aa:Phe,seq:gaa)
     gene            complement(679..751)
                     /locus_tag="ACG8MY_002730"
     tRNA            complement(679..751)
                     /locus_tag="ACG8MY_002730"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(716..718),aa:Asp,seq:gtc)
     gene            complement(761..833)
                     /locus_tag="ACG8MY_002731"
     tRNA            complement(761..833)
                     /locus_tag="ACG8MY_002731"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(798..800),aa:Val,seq:tac)
     gene            complement(849..920)
                     /locus_tag="ACG8MY_002732"
     tRNA            complement(849..920)
                     /locus_tag="ACG8MY_002732"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(885..887),aa:Glu,seq:ttc)
     gene            complement(935..1024)
                     /locus_tag="ACG8MY_002733"
     tRNA            complement(935..1024)
                     /locus_tag="ACG8MY_002733"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(988..990),aa:Ser,seq:gga)
     gene            complement(1064..1137)
                     /locus_tag="ACG8MY_002734"
     tRNA            complement(1064..1137)
                     /locus_tag="ACG8MY_002734"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1101..1103),aa:Asn,seq:gtt)
BASE COUNT          321 a          304 c          263 g          256 t
ORIGIN      
        1 aactaagatg aagacatctt agtcaatacc ggcggccggg gtcgaaccgg cacgccctca
       61 acgggcactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caagttgatt
      121 tattagtact aaggcggtaa ccggatttga accggtgatg aaggttttgc agacctctgc
      181 cttaccactt ggctataccg ccattatact attatcaatc tataaaataa agaataaatt
      241 atagaaactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt cccttgcctt
      301 accgcttggc tataccccaa gaataaaaag ggcgaatgat gggaatcgaa cccacgaatg
      361 ccggagccac aatccggtgc gttaaccact tcgccacatt cgccatggca ggggcagcag
      421 gaattgaacc cacactaacg gttttggaga ccgctgttct acctttaaac tatgccccta
      481 taaaatggag gagagtggat tcgaaccact gaaccctaag gaacggattt acagtccgtc
      541 gcgtttagcc acttcgctac tcctccatgg tggcgcggga cagaatcgaa ctgccgacac
      601 acggagcttc aatccgttgc tctaccaact gagctaccgc gccatatttt aaaattatta
      661 attgaataac aagaacgacg gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag
      721 gcaggcatgt taacccctac accacggaac cagttcatta tggaggttaa cgggatcgaa
      781 ccgctgaccc tctgcttgta aggcagatgc tctcccagct gagctaaacc tccaacattc
      841 aattaaaatg acccgtacgg gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt
      901 aaccgcttga ccaacgggcc aggtaaattt gctacggaga gtaagggatt cgaacccttg
      961 agacagtttg gcaccatcta catgatttcc aatcatgctc cttcggcctc tcggacaact
     1021 ctccagagtg aaagaagctc aatactcttt ctcaaaatga gaactccggc agtaggactc
     1081 gaacctacga catcatgatt aacagtcatg cgctactacc aactgagcta tgccggaata
     1141 acat
//
LOCUS       DAZKNO010000290         1116 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000290 DAZKNO010000000
VERSION     DAZKNO010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.306"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1116
                     /locus_tag="ACG8MY_002735"
     CDS             <1..>1116
                     /locus_tag="ACG8MY_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0431846.1"
                     /translation="SDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNSES
                     QTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDYEF
                     YYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVD
                     ALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIGEN
                     INRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLA
                     LKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLA
                     EVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKK"
BASE COUNT          409 a          168 c          180 g          359 t
ORIGIN      
        1 aatcagattt ttttcatgga attcaaaatg ggtggcaagg gcattggatt attatctctg
       61 cgacatttga aggaatcagc gaagaagaat ttgacaatga gatttgtgct tccatttctt
      121 taaattctga aagtcaaaca atcttagaag aattgatttc aaatgcagat aaaggtgtag
      181 gtagtatttc tctatttatt agacctaaca aagctattag aaagcaatta tttgatattt
      241 ccactgaatt agactctcat caatttaacg aatttagaaa ttctattcgt ctatccgact
      301 atgaatttta ctacacatct aaatcaaatc taaatttctg tatcaatgaa aattatgata
      361 aattggtagg aagactcaat gacaatatag ctagtaatcc tgaagaagac gatgaagctc
      421 tgttgggctc aagaattgac atgtcagata ttttcaagca tatttcggtt gtttatgttg
      481 acgctcttcg agatgttctt agagaaatga aaaacaatcg aaacccagtc aaaagaatca
      541 tggaaacaat tgaatcaaaa atatcatcag ataatgttga gtcattaaaa acaattatac
      601 agcaattaaa tgagactatt accagtgttc ctgaaattag gaaaattggc gaaaatatta
      661 atcggcaact aaatagcata attggttcag tgtattctcc taatcttctt ctttcctcaa
      721 ctatgtcaga tgatatgggt tctttagcaa aatttctttc aatgaagcca gaacaaaata
      781 tcgacttaga cttactaggg ttaggacatc taaatatgat ttatttagct ttgaaaatag
      841 tcgaatttga agcgtgtcgt tcaagagaac tacttaatat tatgttgatt gaagaaccag
      901 aagctcatat tcatcatcat attcaaaaaa ctctgttcga aggattaaat cttcaaaaaa
      961 actatactca gattctcatg accacacatt ctgtacattt agctgaagtg tctgaaatat
     1021 ctcggatgaa tgtattaaaa tcgtttaatg gcaaatctat cgttatgaat ccagcaaata
     1081 atctgaactc atttgctaaa gaaaagttaa aaaaga
//
LOCUS       DAZKNO010000291         1102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000291 DAZKNO010000000
VERSION     DAZKNO010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.308"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..834
                     /locus_tag="ACG8MY_002736"
     CDS             301..834
                     /locus_tag="ACG8MY_002736"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0431847.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          355 a          205 c          202 g          340 t
ORIGIN      
        1 attttttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gaagttgtga
       61 aaaagtcgcc tactttcgag tcgtaagaaa tttttttgaa aatggttcct cacaactttg
      121 cataaatttg acttaatacc gagaagtcat gatccccatc taactccaaa tcacaaggaa
      181 tgagttcaaa aactagcttt tctcaacaaa tattacataa aaaattctat gttttttgtt
      241 aaatttattt gcttttttca aaagtatgtt acagtaattt cccaaggagg ttttacagaa
      301 atgaattttg ttgccatgga ttttgaaact gccaatcatc agccttatag tgcttgttct
      361 cttgcgctag tcatggtaaa aaatagccag atcgttgatg aattctatac attgatacag
      421 cctgagactc cttttttctg gcgaaatgtc caaattcacg gaatccatca agaagacgta
      481 cgcaatgcgc cgaaattccc agaagtctgg caaactatcc aaccatattt tcaaaaaaat
      541 cgtttagtcg ttgcacataa tgctgctttt gatacaaagg tcttagcggg atgcttagat
      601 tattatcatc ttgaacagcc taattatctt tctttatgta cagtgaaaag tagccgtcgc
      661 ctgtttcctg aatttccaaa tcatcgattg aacactgtgt gcgaaaattt gaacatccaa
      721 ttagatcatc accatgatgc attggaagat agtcgtgcat gtgcggaaat cctgctttat
      781 caagaaaaaa aattcgggac agatccattg aaaaaacttg tgactataaa gtaaaagaat
      841 acttatgaaa cacacaaaga ggtcgtgagc ctaccgtttc actccgagtc acaaggagca
      901 atttcaaaaa atagcttctc atgtttatta aatattgtga ctggtgaccg aggagctggc
      961 aggagaacac cgtttattgg agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa
     1021 cagccaaaca aaaatagctt ctcatatttt tcatttggtt ggacttatta ccgcagatgc
     1081 aagcttttgg acaccgttta cc
//
LOCUS       DAZKNO010000292         1098 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000292 DAZKNO010000000
VERSION     DAZKNO010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.309"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..504
                     /locus_tag="ACG8MY_002737"
     CDS             94..504
                     /locus_tag="ACG8MY_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0431848.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            518..874
                     /locus_tag="ACG8MY_002738"
     CDS             518..874
                     /locus_tag="ACG8MY_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0431849.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          374 a          159 c          209 g          356 t
ORIGIN      
        1 tattttgatt ttttttatat tttattagct aataattggt attttaaaag aaattactgt
       61 agaattggat tataattaaa aaggagtgag aaaatgataa aagtatatac ttcgcctagc
      121 tgtacttcct gtagaaaagc aagagcatgg ttgcaagcga atcaacttga atttgaagag
      181 aagaatattt ttgccgaacc gttaacagaa agtgagatca aaaagatctt ggcagcgact
      241 gaaggaggag ttcaagatat tatttccact cggtcgaaaa tttacgaaaa gctgggcata
      301 gattttaacg aattaacttt aaagcaaatg atcgtcttat tcaaagaata tccttcatta
      361 ttgagacgac ctattttgat tgatgaaaaa agaatactaa tcggatttaa tgaagatgag
      421 atccggtcat ttgtccctcg tgaaattcga tgtgttgcca gaaatctgat ctatttacaa
      481 aacaataaca tcttaaattc atagggaggg agccttcgtg gaagtgaaag tctatggatc
      541 agcgcactgt gtaacaagtc tgaaggcgaa acaatggtta gaacaccatc aattgcctta
      601 tcaatttgtt gacttagaag aacgcgatct aagtacagaa gaagcagaag aattatgcag
      661 ttttgaagaa gtgcaagcag aacagctatt tgcgacttgg tctgaggctt ttcacaacat
      721 tgcgattgac ctttcctgtg taggaaaaaa acaactgatt tttttatgca cacaacatac
      781 caatctcctc agacgtcctt tgattattat cgatgaagtt ctatttattg gatacaatga
      841 gcagttgatg gaacagctta tcttaaataa atagacttta actgttttaa tcaagtcact
      901 gagcatttca tacagttgta acagtcgaac acttagcatc aagcgtgtta tgtgttcttt
      961 ttttgacttt atttttctac tatagagaca tgctcaattt atgtggtcaa aattcaaatc
     1021 taggtgggga tatagagtta aaaatatatt gacatgtgta aatttacatg atatattcat
     1081 ttctgttgtt ttaagtag
//
LOCUS       DAZKNO010000293         1066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000293 DAZKNO010000000
VERSION     DAZKNO010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.310"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..>1066
                     /locus_tag="ACG8MY_002739"
     CDS             72..>1066
                     /locus_tag="ACG8MY_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0431850.1"
                     /translation="MSTEIQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSQTIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWF"
BASE COUNT          443 a          159 c          172 g          292 t
ORIGIN      
        1 taatacatgg tatagttcac ttgttgaaaa tcgtgaatat atacaagttt ctatgaagaa
       61 ggagaaattt aatgtctaca gaaattcaat ctcaaaaaat gattattaaa aattggggat
      121 ataaagcaac gaatgtttat attgaaaaat attataaatt tcctagtttt ttaattcaga
      181 attctaaata ctctaaaatg aaagacagct caaaaatagc ctatatgctt tttaaaaatg
      241 aatttcgtag agccttacat gaaaaatgga tcgacgatga tggtctatta tatttggaat
      301 ttacacaagc agacctaatg gacaaattaa attgctacca aggcaaagta aaaaccattt
      361 taaacgagtt agaaacatat aatttgattt cagtcgtaaa aggagaattt aacccaaaaa
      421 cgaaaaaaaa tgaaaaaaat aaatactatt tacttcaacc tgaaataagt gccgatgatc
      481 tttttattca gaattctttc gatgacgatt taagccaaac tattgataaa tcaacctctt
      541 ctcggaatgc ggaaatcgca cgtcgagagg aaactgctaa aacccttgat aatactggaa
      601 atgcggaaat cgcacgtcga gaaaaaacag ctgaaaccct tgataacact ggaaatgcgg
      661 aaatcgcaca agattattat attaataaat ctttagacac taaaagacac ttaaaagaca
      721 ctgaaacaga ccaattacaa aatcaagttt tgctagataa cttcgttgaa atcatgaaag
      781 aagacagcat aaacacattt gtaccagaaa aggtattaag cttgataaaa acattttcta
      841 acacttacga agaagcccaa caaaccgtca ccacaataca caacgcaaaa cataaagccg
      901 aggaacttga gaagacggta gttgtgtatg aagaacttga atcttacgga attgatgcgg
      961 ataaaggatt atatatgacg ttgctgaaag cttatcaaaa acaaaagact gagaaagtaa
     1021 ataatttgca gaatttgatt tttgtgtatg taaaaaactg gtttat
//
LOCUS       DAZKNO010000294         1049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000294 DAZKNO010000000
VERSION     DAZKNO010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.311"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            159..383
                     /locus_tag="ACG8MY_002740"
     CDS             159..383
                     /locus_tag="ACG8MY_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0431851.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            403..690
                     /locus_tag="ACG8MY_002741"
     CDS             403..690
                     /locus_tag="ACG8MY_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0431852.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT          393 a          115 c          202 g          339 t
ORIGIN      
        1 taaataactg atcttataat tttatttaaa cgattatgaa ttcaaaacta ctttttatga
       61 cgttaatcat tgttatataa taaaatgtta tataataaaa ttactaagat tatttaatat
      121 aacaagcaat tttaaaaaaa cgaaaaggaa aaacaatcat gaaaaagaaa gtattaaaac
      181 attgtgttat tctaggaata ttaggaactt gtctagctgg catcggtaca ggaataaaag
      241 ttgatgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa tgttgggtag
      301 attggaatca agctaaagga gaaattggaa aaattattgt taatggttgg gttaatcatg
      361 gtccatgggc acctagaagg tagtggtagg gaggttatta taatggattt tactaaagaa
      421 gaaaaacttt taaatgcaat tagtaaagta tacaatgaag caactataga tgactatcct
      481 gacttaaaag aaaagctctt tctttattct aaagaaatca gtgagggaaa aagtgttggt
      541 gaagttagta tgaaattaag tagttttctt ggaagatata ttttaaaaca taaatttgga
      601 ttacctaaat ctttaataga attacaagaa attgttagta aggaatctca agtatataga
      661 ggatgggctt ctattggtat ttggagttaa ttctttggaa taaaatgaaa atgggactga
      721 agaatcaatt ctgattcatc agtcccataa attatagagc cgaaaaatta tcatatttat
      781 agatttatta atagtaaatg gcataagaat ctttgaacaa cgaaaaaaat gtagctctac
      841 ctaattattg atttgcaaca atttcatctt cggtgaaata agcgaaactt tacaaaaagt
      901 ggctagcatt tgctagcttt ttttgtattg tggagcgtgg gttttgaggg ggattcaagg
      961 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacggaggt atttggctag
     1021 caaatataga gcttgccaag ccctttagt
//
LOCUS       DAZKNO010000295         1040 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000295 DAZKNO010000000
VERSION     DAZKNO010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.312"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..994)
                     /locus_tag="ACG8MY_002742"
     CDS             complement(38..994)
                     /locus_tag="ACG8MY_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431853.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          341 a          173 c          156 g          370 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga
//
LOCUS       DAZKNO010000296         1037 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000296 DAZKNO010000000
VERSION     DAZKNO010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.313"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG8MY_002743"
     tRNA            complement(20..91)
                     /locus_tag="ACG8MY_002743"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG8MY_002744"
     tRNA            complement(113..200)
                     /locus_tag="ACG8MY_002744"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG8MY_002745"
     tRNA            complement(225..298)
                     /locus_tag="ACG8MY_002745"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG8MY_002746"
     tRNA            complement(312..382)
                     /locus_tag="ACG8MY_002746"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG8MY_002747"
     tRNA            complement(384..459)
                     /locus_tag="ACG8MY_002747"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACG8MY_002748"
     tRNA            complement(478..551)
                     /locus_tag="ACG8MY_002748"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACG8MY_002749"
     tRNA            complement(564..653)
                     /locus_tag="ACG8MY_002749"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACG8MY_002750"
     tRNA            complement(677..750)
                     /locus_tag="ACG8MY_002750"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACG8MY_002751"
     tRNA            complement(764..837)
                     /locus_tag="ACG8MY_002751"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACG8MY_002752"
     tRNA            complement(865..937)
                     /locus_tag="ACG8MY_002752"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACG8MY_002753"
     tRNA            complement(957..1030)
                     /locus_tag="ACG8MY_002753"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
BASE COUNT          281 a          285 c          246 g          225 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaatt
//
LOCUS       DAZKNO010000297         1012 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000297 DAZKNO010000000
VERSION     DAZKNO010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.314"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            319..>1012
                     /locus_tag="ACG8MY_002754"
     CDS             319..>1012
                     /locus_tag="ACG8MY_002754"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0431854.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMK"
BASE COUNT          366 a          173 c          179 g          294 t
ORIGIN      
        1 atcaaataac gttattttat gtttgaatag ttatattgag actgtattaa aaaaataaat
       61 ctttcgaaaa ttccacagag attgcattaa aataaatcaa tcgacttcta ccataatgat
      121 taatttgttc atcaaactta ttgacgtgtt tcacgatttt tagtgaaaaa aactgaaata
      181 taaaaacctt attctgtcaa ggaaatagaa atatagtaag aaaaaccttt atttatcagc
      241 ttttttagac aattttttct gttacaatag tcaaggattt gtgaaaaaat caatgaaaac
      301 cgaaaggcgt gtaaaatttt gattaaatca gttgtgtttg atgttgatga tacgatgtat
      361 gatcagcagc agccatttcg aaacgcggtc aaaagagtcg ttccgcttgt ttcagatgca
      421 gatatgcatc cactatatat ccggttccgc catcatagtg atgaaaactt cccaaaagta
      481 atggcaggag attggacact agaatatatg cgagcacatc ggatcagcca atcactaaaa
      541 gatttagact accctcatat tacggaagaa gatggcctct tatttcaaaa aatttacgag
      601 gaagaacttg ataatatctg cttgcatgag gaggtcaaaa aaaccctcga tttcttgaag
      661 gaaaagaatg tcccattagg aattatcaca aatgggccga ctgaccatca gacaaaaaaa
      721 ctgaagcaac ttcaattgaa caattggatt ccttcccgca atatgctcat ttctcaagca
      781 acaggtttcc aaaaaccaga aaaagagatt tttcagctag ctgagaaaga atttcatatg
      841 cttccagaag agaccttata cgttggcgat aattacgaca atgatgttct aggtgcaaaa
      901 agtgctaact ggcaagcgct ttggttcaac catcgagaac gcaaaattga acagactcca
      961 atttgcgata tcgagatttc ttcttttgat cagctgttag agacaatgaa ga
//
LOCUS       DAZKNO010000298         1006 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000298 DAZKNO010000000
VERSION     DAZKNO010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.315"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..885)
                     /locus_tag="ACG8MY_002755"
     CDS             complement(<1..885)
                     /locus_tag="ACG8MY_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0431855.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSP"
BASE COUNT          316 a          175 c          127 g          388 t
ORIGIN      
        1 tggtgaaaag tcgggatcaa ttttcacttc tttttcaaag cgttgtaaaa tatttataga
       61 acgtaaataa tcttctttat tggtatagtt atttttaatc attgtcatca tcatccttat
      121 tgcttcatag acatagcgag ttctaacatt aattgataat tccggataaa acttatctat
      181 gaatattagt atatcttcat ggacttcaaa agaatcaaaa attctaggat tgaagcttcc
      241 tctcagtata cttttagggc tttggtaata gtgatattta gcagatgaat tgacagcaac
      301 tttttcggaa tgatcgatca gcaaataagt agtagcctta tcttccatta atttacccag
      361 tgggaatctt atattatcaa ataaatttct attgaataat ttattatttg cagaatagcc
      421 aattttatta tttataaata agtttttcat tgcttgttct ctatcaaata tttcccattt
      481 tctctcagca attgtatgtt cttttagttg ttctaaactt gaaatctttt ttatttttag
      541 caccgagata tctgctccat aagtttgcgt gccaaaatat agtaactcaa gatagtctgg
      601 ttctatccaa tcatcgctgt caataaagca cacaaaatct cctgttgcag aatcaagacc
      661 agaattccta gccgcactta aaccaccatt cacttgatgt atcactttta atcgcatatc
      721 tttcttttta tattcttctg caatttcccc acttcgatcc ggggaaccat cattgactac
      781 tattatttca atatttgaat gtgtctggtt ggaaatagat tctaagcagt tttctaaata
      841 gtcttctacg ttatatgcgg gtactataac actaatttta tgcactctct tcacttccta
      901 aaaatcatct tttttatttt tacaaatgta tcattaccag taacaatttt agtcttccga
      961 agttaataaa cataaaaaat aacatatcga aaaaaacttt attttt
//
LOCUS       DAZKNO010000299          994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000299 DAZKNO010000000
VERSION     DAZKNO010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.316"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..671
                     /locus_tag="ACG8MY_002756"
     CDS             60..671
                     /locus_tag="ACG8MY_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFQ0431856.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            656..>994
                     /locus_tag="ACG8MY_002757"
     CDS             656..>994
                     /locus_tag="ACG8MY_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0431857.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHF"
BASE COUNT          386 a          136 c          194 g          278 t
ORIGIN      
        1 atgaaataga gagcaatttc aaatgttgct ctctagaaaa aaatgtgagg ggataatcaa
       61 tggatgataa acaaaaaatg tacgaaaaaa atcgcaaaca tagtgaggcc ttaaattttc
      121 gtgtcgataa aaagacttat cttgaactga cattgattgc aaaacaaagg ggaatttcta
      181 gagcagacgt ttgccgtgag cggttaaagg gactcaaata tgaaccgccg ttgttagctg
      241 aagatgatag ggtagtttta accaaacagt taaatcaaat tggtaacaat ttgaatcaag
      301 cggtaaaggc attaaatatc attgctaaat attttagaga agaatctgaa gataaaaaag
      361 aaaagagcct acattttagt tttgatgcta gccgttacaa catttttaaa gatgaacttg
      421 cgaatagcat agcaaaaaag ttgatgatta gttatccaga tgacattttt ttggagtcca
      481 atcggagtgc cccctctttt tttaaattat cagaagtaga aaaagaatat ttcgaatatt
      541 ggatagaaaa gcaaactaga ccgggtacga aagaagagta ttcccaaaaa gttgatcgga
      601 atttaattca ctcaattcag attattgcaa gtttaaaaga tgaggtgaaa caactttggg
      661 agctactata acatcgaaag gacgcaagaa aaatttatcc tcgctattac aatatgcagc
      721 taatccttca aaaaaagaaa aatgtgttgc agtctcagga ataaattgta gtgatgatag
      781 tgaggaagca ctgcaagaaa tgaatttagt acgtaccaag atgaataaat ctaaagatat
      841 agcacaagcg tttcatgtta ttcattcgtt tgataagagt acttctaaag agttaagtat
      901 tgataaaatg cacgaaatag ctgtcgaatt tgcagagaaa gcttttccaa attcgcaaat
      961 cattgttgca agtcacaatg ataaagatca tttt
//
LOCUS       DAZKNO010000300          981 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000300 DAZKNO010000000
VERSION     DAZKNO010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.317"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(680..790)
                     /locus_tag="ACG8MY_002758"
     CDS             complement(680..790)
                     /locus_tag="ACG8MY_002758"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HFQ0431858.1"
                     /translation="MNPNGYEWLRAKGSYPVPKYCNYSETLMEWYQLIIT"
BASE COUNT          327 a          179 c          105 g          370 t
ORIGIN      
        1 taattatccc caatgcctct gaagtaacag acatcactaa ttcaataaca cattcactac
       61 catagtttgt aggaacaaaa atgacaacaa aatctgcttc cttatgtgat gtttttccat
      121 ctgtccaaat tttattacca atgaaatcta gtttaaaatc cctcttagaa actagttcac
      181 tctatatttt tattcagttt cattagttct gtttttttgt cttcagtcac catatacata
      241 tatatcttaa ttatctatcc atttcatcct acgcattctt gcgtaaatac aattcactct
      301 gttgagctga tataaatcat tggtggtaca aagataaaat gataactgtt tctatacctc
      361 caaaaattcc agtaaatata tctatagata gtttcaaaaa aaattgtctt agtttcataa
      421 tatttaagat agtggtacat actttataac aacgtacttt tccaaatatt attttccgtt
      481 atgaacatcc aatatattac aaagtttaaa agtttagatg aactataatc ttcatttctg
      541 cacagtggat aataaattct tatagataaa aaatagcctc ttctaaagtc acaaatacct
      601 tactcccaca ttttaagtaa acactctgca gcattctctt tgttataaaa tgatagatat
      661 tcctcaattt tatctcttat tatgttatta tcagttgata ccattccatt aatgtttcag
      721 aataattaca atattttggg accggataac tcccttttgc tcgtaaccac tcatatccgt
      781 taggattcac taaaagttta ccgtttattt ttcgattctt tttttgaacc gtcccatgaa
      841 cataccgatt caacaagcta aattataaaa attggtgtgc tgtcattatt ttttggaaaa
      901 tttattaatg gctatcaata tacttttgta tcatactaaa tagctcgtca gttcctatat
      961 cggatatacc aatattaaag c
//
LOCUS       DAZKNO010000301          972 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000301 DAZKNO010000000
VERSION     DAZKNO010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.318"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..225
                     /gene="mobC"
                     /locus_tag="ACG8MY_002759"
     CDS             <1..225
                     /gene="mobC"
                     /locus_tag="ACG8MY_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFQ0431859.1"
                     /translation="KAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQ
                     HDVDPGDAQRLSENLKIMQKELQKIWQQLK"
     gene            207..>972
                     /locus_tag="ACG8MY_002760"
     CDS             207..>972
                     /locus_tag="ACG8MY_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0431860.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRR"
BASE COUNT          377 a          212 c          190 g          193 t
ORIGIN      
        1 aaagccaagg cacaaaacgc gcgtctggtg acaccgaaag tggcgccaga cattgctcag
       61 gcaatcgcgc gcgatttagc caaagctggc gggaatatca atcaaattgc caagtggtgc
      121 aacacacacc aacacgacgt tgatcctggc gacgcccaac gcttatccga aaacctaaaa
      181 atcatgcaaa aggagctaca aaagatatgg caacagttaa agtaagccga accacttctt
      241 gtagccgagc gatcaattac gccgaaccac gagcaaccgt caaaacaggg ataaactgtg
      301 acattgatta tgccaaaagc gaaatgaaac aaattcgcat gctgtatgga aaagacgatc
      361 gtgtacaagc acacttattg atccagtctt ttcgaccagg agaaattacc gctgaaaaag
      421 ccaatcaact aggggcagag tatgcgaaaa aaatagcccc tgaccaccaa atcgcgattt
      481 acacacatac agacaaagac catattcata accacattgt cattaactcc gtcaatctgg
      541 aaactgggaa caaatttcaa gcccatggtc aagcattttt aaacaaatgt tatgacctaa
      601 atgacgagat ttgtcttgaa catgacttaa caatcactga acgagggaag aaacccgaaa
      661 aacgaaccat gtctgaaatc aaattaaaag aaaaaaatga acctgtttgg aaagacgaaa
      721 ttcggtttgc gattgatcaa acaatgaaaa acaaaaaaac tcgaacgtat aaccaatttt
      781 gtgattctct taaaacattc ggcattcatt gcttgaatcg tgggaaaaat tttacttacg
      841 aattgatcca caaaaagaaa aaagtacgat ccaacaaact aggaaaggat tacgaaaagg
      901 agacgatttt acatgagctg gacagacgag aacgagccta caatcaacaa cgaaccaacg
      961 agctacgaag ag
//
LOCUS       DAZKNO010000302          937 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000302 DAZKNO010000000
VERSION     DAZKNO010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.319"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>937)
                     /locus_tag="ACG8MY_002761"
     rRNA            complement(<1..>937)
                     /locus_tag="ACG8MY_002761"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          198 a          291 c          210 g          238 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctc
//
LOCUS       DAZKNO010000303          934 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000303 DAZKNO010000000
VERSION     DAZKNO010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.320"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..>934
                     /locus_tag="ACG8MY_002762"
     CDS             83..>934
                     /locus_tag="ACG8MY_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0431861.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEE"
BASE COUNT          334 a          144 c          180 g          276 t
ORIGIN      
        1 aaatagaaat ttagggtaga aaaaaaagtc cattctgtaa aattaaagtt accacacaga
       61 aattttatag gaggactttc tcatgactaa atttactata gaaattatgc aaacactact
      121 caataaaggg gatttagatg aattatttca ggatcattta gagcgagcga ttaattcact
      181 tctacaagca gagctaactg cttttttaga ttacgaaaaa tatgatcgaa atggattcaa
      241 ttcaggaaat tcccgtaacg ggaattattc acgtacattt aaaacagaat atggcgagtt
      301 gaatttaact attcctagag atcggaatgg cgaattttct caacaaacac taccagcata
      361 taagcgaacc aatgattcat tagaaacgac cattatccaa ttatttcaaa aaggcattac
      421 tatggctgaa atctccaaat taattgaaaa aatgtatggt caccactata cgccacaaac
      481 aatctccaac atgagtaaat tggtggctga agatgtttta gcttttaaag aaagaacgtt
      541 agaagccaat tattccgtta tatttatgga tgcgacgcat atccctgtga agcgacagac
      601 tgtttcgaag gaagcagtgt atattacgat tggtattcgt ttggatggaa ccaaagaagt
      661 tcttgggttt actattgccc caactgagtc tgcttatatt tggaaagaag ttcttcaaga
      721 tcttagaaaa cgtgggttag aagaagtttt attagtagtg acagacggat taagctgtat
      781 tgaagaaagt atccatagtg tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt
      841 atctagaaat atcgctcata aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt
      901 caaattggtt taccaagcga attcaaaaga agag
//
LOCUS       DAZKNO010000304          905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000304 DAZKNO010000000
VERSION     DAZKNO010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.321"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(565..765)
                     /locus_tag="ACG8MY_002763"
     CDS             complement(565..765)
                     /locus_tag="ACG8MY_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0431862.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          293 a          171 c          117 g          324 t
ORIGIN      
        1 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
       61 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
      121 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
      181 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
      241 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
      301 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
      361 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
      421 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
      481 ttcagcccat tcaacgacca atgaattttt tcgtacaaaa aaaccgtctt ttctttcgaa
      541 aagacggtta aaagtcaatt tttattaagc tttaacaatg tttgttgctt gagggccacg
      601 ttgaccatct tcaacatcgt aagacactgc ttggccttct tctaaagttt tgaagccgtc
      661 accttggata gctgaaaaat gtgcaaatac atcatttcca tcttcaccag tgataaagcc
      721 aaaacctttg tctgcgttga accattttac tgtaccgtta ttcataaata aattcctcct
      781 gatacgtatc aatgtacgtt ttttcgttgc aataaatatt tgataggcaa aaggagtttt
      841 atctatgtag atatatcgct caaattacgt ttcaaaatag attactcatt taatatatca
      901 tgctt
//
LOCUS       DAZKNO010000305          885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000305 DAZKNO010000000
VERSION     DAZKNO010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.322"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..839
                     /locus_tag="ACG8MY_002764"
     CDS             99..839
                     /locus_tag="ACG8MY_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431863.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT          328 a          161 c          181 g          215 t
ORIGIN      
        1 catcgctatt ttcttaaaat aatcagcgca ttgctactga aaacaatgaa tctgtgctat
       61 tattgagaaa agttccattt ttgagaagga tggtttgaat gaaagaaatc agacgaaaac
      121 atttccgttt tcctgcgtac gatgatgagt taggcgtaaa attaaccaac gacaagaagc
      181 gagtattatt tgacggacaa gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac
      241 agatgaaaga atttcatttc acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg
      301 caacaaatgg attgaagtca gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat
      361 taccggatta taccggccat taccaatcag aaatcacatc gacaggacga aaaaaacttt
      421 ttggtgattc ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc aagcatacta
      481 cacgaaacgc atctgttgac acgaaggtgc gggagcgttc ttattttgtt cctaaatatg
      541 tacccgcatc gattatccca gaccctagag aaagcaaaat ttctgaggat catttaatcg
      601 aatcgatgaa aaaaacggat gactcttatc tgttgttcga tacagaaccg acgccgtatc
      661 aagaaaaaag aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg
      721 aaacaaagca gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta gaacgctcat
      781 tacaaggatt gatcgatgat caaggaaact ctttgggaga aaatagctat tttaaataag
      841 tgatatacaa atttatattc ggctacagtt ggaaaggaaa cgctg
//
LOCUS       DAZKNO010000306          882 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000306 DAZKNO010000000
VERSION     DAZKNO010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.323"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..379
                     /locus_tag="ACG8MY_002765"
     CDS             <1..379
                     /locus_tag="ACG8MY_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0431864.1"
                     /translation="MHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARILNLS
                     EVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISA
                     NAGPVTIPVPGGGFTWDNTITNW"
     gene            352..795
                     /locus_tag="ACG8MY_002766"
     CDS             352..795
                     /locus_tag="ACG8MY_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431865.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT          342 a          139 c          137 g          264 t
ORIGIN      
        1 tatgcattat acctatccta atttaaacaa cagtcaaatt gaatcaaaaa gtcttaatca
       61 agatcaaata ataaaatatg caaattactg tggtgtttat cctaaaaaag tagcacgaat
      121 actaaacctt tcagaggtcc aaacacaact aagaaaagga aatgccattt atatgggaac
      181 tgctggttat ggaaattata gtaacagccg tcatgcactt gcattatttg ggtgggtcaa
      241 tcaaggtggt tatcaaactt actatgtgtg gaatccttgg tataactacc caactgtaat
      301 aagtgcaaat gcaggaccag ttacaattcc tgtacctgga ggtggtttta cttgggacaa
      361 taccattaca aattggtaaa taaacccatt gctttagtta tgggtctagt gttagttctt
      421 ttcttaacta gttgttcaaa aaaacaagaa atacaaaaga ctgatctgat tccttttcta
      481 ggaacttggc aaactaaaga tcattcagaa cgtctagtaa tcactaattt aaatacttca
      541 aaaaatatag aacaatatac catcttagta aaagagaaca atcataaaat tcaaatcatt
      601 gataaaaatt taaatataaa tgaacaagtt ttcatttaca aagcaacaaa taatttacta
      661 acactgcgct ctaataaaaa acaattagat tacactttta gtttggattc tccaaaaaag
      721 ctcgtattta ttttgacaac gaatgatggg acagaaggga cagctaaacc aattgaattt
      781 tttaaagctg attgatgaat agttgtttga tattacaaaa caataatttc accgaaggtg
      841 aaacaagcga aactttacaa aaagtggcta gcatttgcta gc
//
LOCUS       DAZKNO010000307          873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000307 DAZKNO010000000
VERSION     DAZKNO010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.324"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..626
                     /gene="bacA"
                     /locus_tag="ACG8MY_002767"
     CDS             192..626
                     /gene="bacA"
                     /locus_tag="ACG8MY_002767"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFQ0431866.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT          322 a          121 c          126 g          304 t
ORIGIN      
        1 aaaaatcaca aagtgagccc ctgtatcggt tgatacaagg gctacttggc atactgtaca
       61 ttatgtctac taacctttaa aataacacgt tttataaaat aaaccctctt gacaatattt
      121 aattttctga ttattctaaa tatatacaga aagcgctttc ctaggatttt atatctaagg
      181 aggaagtaac aatgaaaaat aaaattatta aaattattac ttgtctattt ttgttcggta
      241 tgattgttgg agcaagtact ccgttaaatc tgtttccttt tagtgctaga ggtataaaag
      301 tggaagctgc aagttcacga tataatcata atcatcgtgg atttacttgc tataaaatga
      361 actattatgt tactaataaa atgtgtagag atttgaaaaa aaattataat aagctaaaga
      421 ctcctgctca aattgcatct tttattccta ttggtggagt tggaacatgg gttattacaa
      481 atgcttttag tagtgctgtt gatgcaatga atgtctttgt aagagctgca aatcaaggaa
      541 aaggtgtaca attaacatat aatgctcact ttagcaatac aacatcatat caatacaatg
      601 attacgctcg ttatgtaata aaataatcag aaagtgaaac tatctcatga ataaaaagat
      661 tttagctgta attatttcaa taatagtttt aatcactatg ttcttcatat ttaggctaat
      721 tttcaatata agcttacaaa aaagtattct ttatctaatt cctatagcat ttatcttagc
      781 aatttttaga agtatttacg gaaataaaaa ctctaaatag ctaaaaataa atcatactaa
      841 attttacaaa aagtggctag cattttctag cca
//
LOCUS       DAZKNO010000308          849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000308 DAZKNO010000000
VERSION     DAZKNO010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.325"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            30..779
                     /gene="pnuC"
                     /locus_tag="ACG8MY_002768"
     CDS             30..779
                     /gene="pnuC"
                     /locus_tag="ACG8MY_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0431867.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT          236 a          163 c          166 g          284 t
ORIGIN      
        1 acatttagtg tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc
       61 agctaaaagg ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg
      121 tcatgactct tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca
      181 cgcttggtgt attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag
      241 gtattttgtc tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta
      301 tcggtgaaca gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat
      361 ggaatcataa tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa
      421 tcatcagtac tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg
      481 atcctcggcc gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca
      541 gtttcttgcg ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg
      601 tcctttggta tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct
      661 ctgtttactt gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag
      721 aacgtgaacg tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa
      781 aaaactgagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
      841 acggcggaa
//
LOCUS       DAZKNO010000309          840 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000309 DAZKNO010000000
VERSION     DAZKNO010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.327"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>840
                     /locus_tag="ACG8MY_002769"
     CDS             <1..>840
                     /locus_tag="ACG8MY_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0431868.1"
                     /translation="LKDAKVVSGEQRINREDLSDEFKNVVSLEATNNTKRNILFSSGV
                     FTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGI
                     FSGKKQETYTGLSSDFSENMVFVDYSTSQEILNNSENNEIANKILMYSGSAESTDLAL
                     NKLKELKIDESKYFVEKDSNAFEESLESVSGIKYIIKVMTYSIMLGGMVVLSLILILW
                     LRERIYEIGIFLSIGTSKIQIIMQFIFELIFISIPSIISSLFLGNVLLKVIVDGFINS
                     EDSM"
BASE COUNT          343 a           74 c          143 g          280 t
ORIGIN      
        1 ctaaaagatg ctaaagtagt tagcggagaa caaagaataa atagagaaga tttatctgac
       61 gaatttaaga atgttgtttc actcgaagct acaaataata ctaaaagaaa tattttattt
      121 agtagtggag tatttacaat taaagaaggt aaaaatatag aagaaaatga taagaattca
      181 attattgttc atgaagaatt tgctaaacaa aacaatctaa aattaggtga tgaagttgat
      241 cttgaattac tagatattga aaaaagtgga aaaataaaaa gtcataaatt taaaattata
      301 gggatctttt ctggtaaaaa acaggaaaca tatacaggat tatcttctga ttttagtgaa
      361 aatatggtgt ttgtagatta ttcaacaagc caagagatat taaataattc agaaaataat
      421 gaaattgcaa ataaaatttt aatgtattct ggtagtgcag aatctacaga cttagcctta
      481 aacaaattga aagagcttaa aattgatgag tcaaagtatt ttgttgaaaa agattctaat
      541 gcgtttgaag agtctttaga gtcagtgagt ggaataaaat atataataaa agtaatgact
      601 tattctatta tgttaggtgg gatggttgtt ctttcattaa tcttgattct atggttaagg
      661 gaaagaattt atgaaatagg tatattctta tctattggaa catctaagat acaaattata
      721 atgcaattta tattcgagtt aatattcata tcgataccaa gtataatatc ttccttattt
      781 ttaggaaatg tattactaaa agtaattgta gatggattca ttaactcaga ggattcaatg
//
LOCUS       DAZKNO010000310          825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000310 DAZKNO010000000
VERSION     DAZKNO010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.328"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..527
                     /locus_tag="ACG8MY_002770"
     CDS             54..527
                     /locus_tag="ACG8MY_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0431869.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            536..763
                     /locus_tag="ACG8MY_002771"
     CDS             536..763
                     /locus_tag="ACG8MY_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431870.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          281 a          125 c          148 g          271 t
ORIGIN      
        1 tacaataatt attctgctat attttagcta tcaaagataa aagagggata aaaatgaaat
       61 ttaaaacatt gctattaaaa tttgtcgttt taggtataag tgccgtattt attttgttcg
      121 gcgtactttt ttatactcag atcgctacaa gcgaaaaagg attcaatttt gattggcaga
      181 ccacattatt tgtttctgtc atttactttg cccttctcct agggatcgtt attgcttatt
      241 tcttattgaa actattgaaa aaaatcgaag ataaccaagc tttttccact caaagcttaa
      301 acatactaaa aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt tttggcttac
      361 ttcctaaatt tatgaaagta gcggatttag acgatgctcc aggtttgatg ttggtaggaa
      421 tcgctgtcgt attcattcct tttgcagtat acacattaac aagcgtgctg gaaaaactgc
      481 ttgaacatgc gattttactt aagacagatc atgatttgac agtgtgaggt gcgacatggc
      541 aattatcgtt aatttagatg tagtattagc tcaaaaaaaa atgagtgtga ctgaattgag
      601 tgagaaagtg ggcattacga tggcaaacat ctctattcta aaaaatggaa aagcaaaagc
      661 catccgtttt tccactctcg aaaaaatctg ccaagttttg gagtgtcaac caggtgattt
      721 gttgacctat aaaaatgact cgaatactat accagaagaa taaaataaag tagaacaaac
      781 agtaaataaa aaataatctg aatgatagat gtgtgagact tttag
//
LOCUS       DAZKNO010000311          805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000311 DAZKNO010000000
VERSION     DAZKNO010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.330"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          276 a          114 c          138 g          277 t
ORIGIN      
        1 cctctttttt gaccagattt tgaccagtct aatttacatt ctaatatatt catatgagct
       61 aattttttga ttaaatggta agtttttcct tgtttgtttt agtttaaact agtgtgtaat
      121 ttaaaaatct atatggaagg gtctgtgggg taatacttaa ataaaggcta ggaacctgat
      181 aacatcaagg ttcctagcct ttttataata aaatgaagcg aaaataattt cttttaaaga
      241 gccgagatta tcagctatat aagagtgaat ttgatgcaaa aaacagagcc gaaacagtct
      301 tggtgttcgt agctctgctt atatgtttcg aatttaagac aaagagttgg caaaagatgt
      361 tgataaggaa gataagataa ctgtgttata taaactaggt aactaactgt aacaagctaa
      421 ttttaagaga ttcttattag aagacgttta ggaatataac taagaaaatg attatacagg
      481 taattgtcat ttcaataatg ttttctaatt ttcgcgtctt tgttttaaga aaaaggccag
      541 aatcagttgg aaaaaagttc gtacgatagt gaagaggggc aaaatttcct actaatattc
      601 ccactaacaa gagataaaac ggccaggttg gtgtgattcc tctataaaaa aatgcccatg
      661 ctaccgaaat cccatagatt aacaactgaa caatttttac aatgaaaaaa ttgttctgcc
      721 attactcaaa gtctcttcct ccttgtatcg tttatttgtt tcattttatc attatgatag
      781 gcgcttgaac aatccatttt gtatt
//
LOCUS       DAZKNO010000312          805 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000312 DAZKNO010000000
VERSION     DAZKNO010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.331"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG8MY_002772"
     CDS             134..307
                     /locus_tag="ACG8MY_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431871.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          271 a          130 c          101 g          303 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaa
//
LOCUS       DAZKNO010000313          803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000313 DAZKNO010000000
VERSION     DAZKNO010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.332"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..691
                     /locus_tag="ACG8MY_002773"
     CDS             176..691
                     /locus_tag="ACG8MY_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFQ0431872.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
BASE COUNT          273 a           97 c          175 g          258 t
ORIGIN      
        1 tttgaatttt aatatggtta atatttttct gcagacagga ttgtgtaatc tcatcaagaa
       61 tactttacat gccatctatt tttgctaaaa cattgttgtt atatcttcaa gaggtaggga
      121 attttattgc tttaaatgag gtcgagcgaa ttaaaaaatt ggtaaaatat ataacatggt
      181 ggtagttatg gtaaaagtgt ttgatataat taagttttcg gatttcagag gatatactgc
      241 tgttgcattg gataatgaga ctcttttctc gattggtatt gagtttcaca ttaagcaaat
      301 aaatcaagga tttagtcata ataaatatac tatgcgaggg ctacattatc agagtggaga
      361 gcatgctcaa ggaaagatag tgatgtgcct acatggtgaa attatgaata tcgcacttga
      421 tattcgacaa ggatcttcca cttatggatg tgtgtatgtc aacacattga gtgaaagcaa
      481 tggtaaatgt gcgtatatcc cacgtggata tgctcatggc tatcttacta tggaggacaa
      541 tacgctagtt cagtggtgcg tagataatga tttcaattct aatgctgcca aatgtataag
      601 ttatgaaagc gtagatattg aatggccggt cagtcggcag tgtttcgtta taagtgagaa
      661 agacaaaaat gtggaaagat taagcttttg attaaaatga gctgtggaga aaagaaggat
      721 actggaaaat aagaagaaca ataaccgagc aaatgctcgg ttatttctat gaggaataca
      781 taaaactaaa tttagatata gat
//
LOCUS       DAZKNO010000314          795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000314 DAZKNO010000000
VERSION     DAZKNO010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.333"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            383..>795
                     /locus_tag="ACG8MY_002774"
     CDS             383..>795
                     /locus_tag="ACG8MY_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0431873.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLE"
BASE COUNT          275 a          149 c          123 g          248 t
ORIGIN      
        1 aaaactttat aacaactata acaaaaccga atgcaaaaca gaagttctat aagttataat
       61 catagttgtt aggtgattca tctagtaaaa acaccagatt tcaaatttca cacaatttaa
      121 actagatcaa tcatcaacct tcttcaaatt gttagaaccc tttcccgagg agctgcagca
      181 taagcaaaaa aacaaacagg aagccaaatg gctaagacat tttttctatt agtctagttg
      241 tttctcgtcg caagtttaag actttagaaa gttatttcta atgatgtttg ggttgttttg
      301 atcaatgctt atgcacagct ccttacctat tatttatctc aacagatcta acaaattaat
      361 ggactattta aggagttttt ttatgcttac gatctattct tctcgcacac atagttacca
      421 tctgatggca aactggctga aagaccatca aatgccttac agagaacgaa ttttcacacc
      481 taaacagcct ttaacaagga aagagatatt ttatttattg tcattaacag aaaacggatt
      541 tgacgatcta ttagctaaac gctcgattga ttataaaaat ttgaaagacc aaatcgacca
      601 atgtacgacc tcagagcttg tcgatctgat cgtctcgcat tttactttgt tacaacgtcc
      661 catcattact gacggaaaga aattattagt cggattcaat gaagatagca tgcgtctttt
      721 tatccctgta caatggcgca gaagtcaact gaaatctgtc tataacgatt ttacatgggt
      781 aagcaatcta gaaaa
//
LOCUS       DAZKNO010000315          795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000315 DAZKNO010000000
VERSION     DAZKNO010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.334"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..637)
                     /locus_tag="ACG8MY_002775"
     CDS             complement(41..637)
                     /locus_tag="ACG8MY_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0431874.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          301 a          149 c          121 g          224 t
ORIGIN      
        1 ttaacattgt caatcaatga ctaattttac aaaataaata ttatccaaga agaccactta
       61 gatattgacc tccataaatc aaggccatac tatataagaa aatcgacagt ggtttagcaa
      121 tcaaaatgat tagtgtatat cgtttcactg acatgtttgt caatccagcc atcaaacata
      181 acgcatcgtc aggagcaact ggaagaaaaa tagctagagc aaacagcttc tcaaaacggt
      241 tttgattatc cagccagcca atatatttgt tatatgtctt atcgctaatc atggaaagta
      301 tgaatggctt tccataacga cgccctagca gaaaaatgat aatcgaacca atgcagattc
      361 caatatagtt atagataaat ccagcataag ggccaaatat caggacacca gctgcagtac
      421 tgatccctcc aggaatgatt ggaataacta cttgcaggat ttggatgaag acaaaaataa
      481 tcggtcctaa aatgatggaa tcacctacta atcctcttaa cgcattcaaa tctttgaaaa
      541 cacctaaatt aataaaataa atcgtcagcg cgattgatag gcctaatccg ataatggata
      601 tgaaatttat cagttttcga gaggcagcaa tattcattat tcatcaactc cttagaggtt
      661 ctttgttaac tatactttac tagaaaagca actaactgcg ccattttttg atatgacaga
      721 aaacaaacaa ttttgtaagt tcatcataag tataaaagat tgatctcaaa aaaacatcca
      781 actaaagact tattt
//
LOCUS       DAZKNO010000316          782 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000316 DAZKNO010000000
VERSION     DAZKNO010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.335"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..385
                     /locus_tag="ACG8MY_002776"
     CDS             2..385
                     /locus_tag="ACG8MY_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431875.1"
                     /translation="MSEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSE
                     IEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYA
                     VVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            399..>782
                     /locus_tag="ACG8MY_002777"
     CDS             399..>782
                     /locus_tag="ACG8MY_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431876.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVV"
BASE COUNT          285 a          105 c          142 g          250 t
ORIGIN      
        1 aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata aagtaattaa
       61 tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt ctgtaacagc
      121 tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg aagttttaaa
      181 caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc ctcctcaagt
      241 taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag caggagagca
      301 ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg atatcaaggt
      361 caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata gtaccatcaa
      421 atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc gcatcggtaa
      481 ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa aatgataatc
      541 aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat ggcaaaattt
      601 ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa tatgatgaag
      661 atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc tcagattacg
      721 atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg aaatctgtag
      781 ta
//
LOCUS       DAZKNO010000317          760 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000317 DAZKNO010000000
VERSION     DAZKNO010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.336"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            96..332
                     /locus_tag="ACG8MY_002778"
     CDS             96..332
                     /locus_tag="ACG8MY_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0431877.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            329..727
                     /locus_tag="ACG8MY_002779"
     CDS             329..727
                     /locus_tag="ACG8MY_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0431878.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          283 a          116 c          133 g          228 t
ORIGIN      
        1 aaaagcaaaa taaaagtata tacattgatt aaaagtatat acttggatat aatgtactta
       61 ctatagaatg agaatggtat agagggtggc gaaatatgaa aaaaatgaaa aagtataaaa
      121 ttagaaaatc tggcaatagc gatgtaacaa ctatcccacc agaagtaaaa gaattcatgg
      181 gtgtgcagac aggtgacgca atcagttatg tttttcaatc tgatggttct gtacgaatga
      241 tcaaagcaca agaagaacct gatattgatt cattagttga ttcgatcatg aatcaatatg
      301 aagacgcgtt aaaagatttg gttgatttat gaggtatctt tcgactaaag aaattatcaa
      361 aatcaacgcg aaagttattt tacgtcactc gccaggcgaa atgatcggtg taaaagatgc
      421 aaatgcatta gatatggccg tcaaacagcc ttcacaagca gtattcaatc aagaactcta
      481 tcctgaaatc tatgaaaaag cggcgatttt agcaattaat ttagctaaaa aacatccttt
      541 tcataatggc aataaacgaa ctgctcttgt tgccatgttg ttgtttcttc aattgaataa
      601 ttgtccagtt gctttttctc gtcaagaagc tgttgatttt atcataatga ttaccactag
      661 ttcattggat ttcgattcac taaagtccaa gataaccagt tatttacgac aaaccgcaat
      721 aaaatgaacg aattaaaaac tttatttctc aattagatca
//
LOCUS       DAZKNO010000318          724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000318 DAZKNO010000000
VERSION     DAZKNO010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.337"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..72)
                     /locus_tag="ACG8MY_002780"
     CDS             complement(<1..72)
                     /locus_tag="ACG8MY_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0431879.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYI"
     gene            230..493
                     /locus_tag="ACG8MY_002781"
     CDS             230..493
                     /locus_tag="ACG8MY_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431880.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            517..705
                     /locus_tag="ACG8MY_002782"
     CDS             517..705
                     /locus_tag="ACG8MY_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431881.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
BASE COUNT          282 a           91 c          148 g          203 t
ORIGIN      
        1 tatgtaatag aaaagaatgc ttatcataat aaaaaaagga atataaaaaa caaattgacc
       61 gatcgcgatc atactattgc ctccgataaa taaagtccat gatacaaaat aacactttgt
      121 gcgaaatggc gcaagcagtt ttgtggattt atccggtaaa atctgtatcg attttgaaat
      181 tagaggagaa acttgctata atcaaagtga aagaaggagt gggattttca tggatcatgt
      241 agtgttagaa atcgatttgc atttgatcaa taatattcgg gtgatttact atgttgtaag
      301 taacagtgtc gaacaacgtg tactgacgaa taaaataaat ggtattttag caaagaaaga
      361 cgttcaccga ttcaataatg gtgaaggaag ttattatagt atcccagtag aaaaaattat
      421 ttatacgaca gtcaaagtca gagaagatct agataccaaa aaagcatatg aacctatttt
      481 tacaacttat tgattgacag cagatggagg aaaaaaatga atccatatga acaacttatt
      541 caagatttga tcgatggaaa aatcgaaaag attgatgtaa aaagagaaga attaatggct
      601 tttcgagaag cgtggctgaa gttggagaat cgtaaatatg tggtgggaaa agcctcactg
      661 aacggcaatg tgacttatca ttacgatccg acacgtttat tttagtatga agtattcaga
      721 aaag
//
LOCUS       DAZKNO010000319          720 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000319 DAZKNO010000000
VERSION     DAZKNO010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.338"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(122..>720)
                     /locus_tag="ACG8MY_002783"
     CDS             complement(122..>720)
                     /locus_tag="ACG8MY_002783"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0431882.1"
                     /translation="LFFKRMIDIVGSIIGLILASPLMFYVAYRIRKEEPGSPIFFCHT
                     RVGKNGKTFKMYKFRSMCLDAEEKLEELLDQNEIEGAMFKMKEDPRVTKIGKIIRAKS
                     IDELPQLWNVLKGDMSLIGPRPPLEREVAEYTRYDLQRLYVKPGCSGLWQVSDRNNVS
                     FHKMVELDLEYISHRSLMYDLRLILKTFYVIVKPNGAY"
BASE COUNT          233 a          154 c           83 g          250 t
ORIGIN      
        1 tttctttttt tagattttct acaagtgatt caaaccctcc ataattacag ttattacttt
       61 tgaaacgata atatgaacat gtgtaatctt ttttattaac aatccatcat tctatcccta
      121 actaatatgc tccattgggc ttgacaataa cataaaacgt ttttaaaatc aacctcaagt
      181 cgtacattaa acttcgatga ctaatatact ctaaatccaa ctcaaccatt ttatggaaac
      241 taacattatt tctatcacta acttgccaca aacctgaaca accaggtttt acatacaagc
      301 gttgaagatc atatctcgtg tactcagcta cttccctctc caaaggtggc cgtggaccaa
      361 ttagactcat atcgcctttt aaaacattcc atagctgtgg caattcatca atgcttttgg
      421 cacggataat tttacctatt ttagtgacac ggggatcttc tttcatcttg aacattgcac
      481 cttctatttc attttgatca agtaattctt ccaatttttc ttcagcatct aaacacatcg
      541 aacggaattt atacattttg aatgtttttc catttttacc aacacgtgta tggcaaaaaa
      601 atatcggact tcctggctct tcttttctga ttcgataagc tacatagaac atcaaaggag
      661 aagctaaaat caaaccaata atgcttccta caatatcaat cattctttta aaaaataaat
//
LOCUS       DAZKNO010000320          697 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000320 DAZKNO010000000
VERSION     DAZKNO010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.339"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>697
                     /locus_tag="ACG8MY_002784"
     CDS             <1..>697
                     /locus_tag="ACG8MY_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0431883.1"
                     /translation="RTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSI
                     IAMYYVTDITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGL
                     WKSAIEIFKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLL
                     TLLTFLFIQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFI
                     FWGYLGSIQKTIS"
BASE COUNT          226 a          108 c          117 g          246 t
ORIGIN      
        1 cacgcaccac attagtttgt ttattaatag caacttttgt ttttagtttc tttacaagtt
       61 atagtagtcg ttttcttaaa actaatactt taaaaagatg gctaactgca ttagtagtag
      121 gattgctaag tataattgcg atgtattatg taacagatat aactgctcaa gcatgggcac
      181 atgttccgca tattacagaa ataaaaaaag atatatctga ttcatcagga acaactagca
      241 gcaataaagt tgatttaaaa cgccctgaca ctgatggcgg ggatgtttct aatttaaggt
      301 ttggactttg gaaaagtgca attgaaatat tcaagagtaa gccgatatcc ggcggttctc
      361 caggatacta tattgaatac gcacatgata aactaccata tacattaatg ggaaaagata
      421 ctttaactgc tcataatttt attttcttag ttatggctgc tacaggagga ttaggtctat
      481 tgacattgtt aaccttctta tttatccaat gctttaaaac tttgcgttat tgtttcacac
      541 aaccaaatct agtaaaagat aagcactttt ttgctatttt aatgattctt acaatagcag
      601 tttcggcatt atttatcaca gaactcattt tggttagtac gataggttca tttatttttt
      661 ggggatatct gggttctatc caaaaaacta tatctaa
//
LOCUS       DAZKNO010000321          695 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000321 DAZKNO010000000
VERSION     DAZKNO010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.340"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..627)
                     /locus_tag="ACG8MY_002785"
     CDS             complement(52..627)
                     /locus_tag="ACG8MY_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFQ0431884.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          228 a          151 c          125 g          191 t
ORIGIN      
        1 tgaaaaaaca gagccagatt agcattattt tgctaatcca gctctgttta ttcagcctat
       61 ttttcttttt gaatatatca agcgttgggg caacatagtg atgactaatg aaacaacgac
      121 gatagacaac aagcgatcaa ggtagtctgt acccgcctgt accaagatca cacttgttgt
      181 ctgacttaaa cctaacccat gtaatagctg aacgatcata ctggatcctg aggaggtgat
      241 cccgccaaat aactgaacgg taatgatcga agaaacgatc gttccaggta ccgacaaaat
      301 caaagcgagt aataacagtt tcaagctgtt tttatctttg ccgagatatc ttggcaaaat
      361 caatccagca agcaagcctg tgagaatttg gattggcgaa taatacagag aaaaaatatc
      421 tgtagtcact ccacttaaaa taccgctgga caatcctgtc agcattccag gtacaggccc
      481 aagcaatgca ccagcaaaaa tcgttccgat agaatcgaga tagattggca aacgaagcca
      541 taaagcaatc gttcctccaa tataattgat cccaatacac aaagccatca ctgtaagtac
      601 gcgagttgcc attttgtttt tattcatttt gttagcccat ctttaacctt tccaaatctt
      661 tcataatcgt ttcttcttgt gcatttaaaa gaacg
//
LOCUS       DAZKNO010000322          694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000322 DAZKNO010000000
VERSION     DAZKNO010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.341"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>694
                     /locus_tag="ACG8MY_002786"
     CDS             <1..>694
                     /locus_tag="ACG8MY_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFQ0431885.1"
                     /translation="GLLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGS
                     TSFEYIASLFDDDRIQRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNE
                     NNWVNCFYANTTFEIEFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLER
                     NLAMLKLANHVGKGWFAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHT
                     IDLYEETTELSAL"
BASE COUNT          261 a           86 c          123 g          224 t
ORIGIN      
        1 tggattgctt ttttctaaag gtgttatctt agttgaagga gatgctgaag aaatacttat
       61 tcctcaaata gtaaaaaaat tatttggtat aaacttagac gaacttggaa taggtttgat
      121 taatgttggt agtacttcgt ttgaatatat cgctagcttg tttgacgatg atagaataca
      181 gagaaagtgt gctattgtaa ctgatttaga tgaacaggca atcccatcag aacatcgatt
      241 gtataaaagc actgcacaag agaaaggtca agcaagaaaa gaaaaattat cctccctttt
      301 taatgaaaac aattgggtta attgttttta tgctaataca acatttgaaa ttgagttttt
      361 agatgtgaat aaagaaaata ttctaactta tgtcagtcca attatagatg acgcattcaa
      421 agataaaaaa acaataactg cttataaaga agaaatacaa agtgaacaat atttagaaag
      481 aaatttagcg atgttaaaac tagccaatca tgttggtaag gggtggtttg caattgagtt
      541 aggtgaaaaa attgactttt taatcaaaat tcctaagtat attcttaatg ctattgcttt
      601 tgcatgtcag gaaatagtag atctctcaat ttataaaaaa attctactcc atacaatcga
      661 cttgtacgag gaaactacag aattgtcagc ttta
//
LOCUS       DAZKNO010000323          692 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000323 DAZKNO010000000
VERSION     DAZKNO010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.342"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..627
                     /locus_tag="ACG8MY_002787"
     CDS             <1..627
                     /locus_tag="ACG8MY_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0431886.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLS
                     YNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISN
                     AFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          218 a          154 c          125 g          195 t
ORIGIN      
        1 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
       61 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      121 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      181 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccactcaaa
      241 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt tgatgagctg
      301 ttaagctaca acgaagaatt gcgaactgcg tatcattttt gtcaattatt acgctattat
      361 ttcgttaaac gaggtacgga aggctttcaa gaaaccctta aaacaatttc atctacgtta
      421 cctcagtcgt ttaaaaagaa attcacgatc ttccatcgct accaatcggg tatttcaaat
      481 gcctttaagg tgtcccactc aaacggcgtc actgaggggc tgaataacaa gatcaagctg
      541 attaaacgaa ttgcgtttgg ttatcgcaac ttttataact ttcgggcacg gatctattta
      601 caacaaggtc tgatctttga aaactaaaaa aatatgcggc gcacagaacg cgccgcatat
      661 cggactaagt tttattcaaa tttgtcttca cc
//
LOCUS       DAZKNO010000324          659 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000324 DAZKNO010000000
VERSION     DAZKNO010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.343"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(338..562)
                     /locus_tag="ACG8MY_002788"
     CDS             complement(338..562)
                     /locus_tag="ACG8MY_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431887.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          240 a          100 c          118 g          201 t
ORIGIN      
        1 gtggagaaaa ctatggtttg gcgcagattc tatcaggttt gatggaacga agacaacctc
       61 ccgacatcaa gtcaaaagct acttgagatt ttgatctgaa aataaaaaaa gaaaacaagg
      121 taggaacatc aatgtttcta ccttgttttc gtaatcaatg ggaaaaaact aagagtggtg
      181 tacgtaatca ataaaaagga tggtgacaac agtacctctt tactctctga aagcatcata
      241 actcgttttt cctcattatg ccaataaatt gttgttatac cacatataag gatttgtaat
      301 agctaaaaac agacaggaaa gttgaaatct gtgaatctta ttttgtatgg aaggatttat
      361 agaacgcaaa aatcaagata aagatgaata aaaatcctgt ttcggctata tattgcgtat
      421 ctacgccaat aaaacgagtt tgttctgtca tagccatata aagcaaaggc aaaaatacaa
      481 ctacagcgag ataagatccg atgatttctc gaaaagtttt ccaggaagta tgtttcctgt
      541 ttttatattt atccgatttc attgaatagt catagcctcc ttattctttc atatgttgaa
      601 attccagtat aaaaactgga aggaactgta cgttttttag aataaaaatt ataagggat
//
LOCUS       DAZKNO010000325          637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000325 DAZKNO010000000
VERSION     DAZKNO010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.344"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            19..351
                     /gene="traE"
                     /locus_tag="ACG8MY_002789"
     CDS             19..351
                     /gene="traE"
                     /locus_tag="ACG8MY_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0431888.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQNKTVVQCKKYI
                     IQKDKVSEEMIYYVGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT          230 a           88 c          136 g          183 t
ORIGIN      
        1 aagtgggtgg attattttat gtcatataca ggaattttga gtctcgaaga tatttgtcac
       61 tacggtaaac ggtgtaccgc tacagagaag atcacgaaga aactatcaac tggccaaaat
      121 aaaaccgttg tgcagtgtaa gaaatatata atccaaaaag acaaagtctc agaggaaatg
      181 atttattacg ttgggaaaca aaagcaaata attttaaaag atcctatccc tttaaaagag
      241 ctatatccaa caatcaaaca tgtttatgac caaaacggtg tattgatagg tcgaagaaaa
      301 aatggagtct tacgttgtac cgcaaaaggg atggggcgtt tgatcagctg aattgatcat
      361 tcttataact tgtattttat cgtttttcgc ttgcttgaaa atattgtcga gacttctaaa
      421 agaacttaga atcactagaa aaataaggag tagacttgat ctatcaacaa ttcccttcgg
      481 ggagtgatgg ggtaggtttt tacgattggt aacgtaaaaa attggtcaat cgagtaaatg
      541 cccttgtttg gatagtggtg aaatgaattt gtgtaaataa tatgcacaga gtaaacaaga
      601 gggaatttag aagttagaca agttagaata gcgccta
//
LOCUS       DAZKNO010000326          625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000326 DAZKNO010000000
VERSION     DAZKNO010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.346"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..528
                     /locus_tag="ACG8MY_002790"
     CDS             <1..528
                     /locus_tag="ACG8MY_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0431889.1"
                     /translation="KAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKF
                     VEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEE
                     FRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSL
                     TSDLPKISLRNWLEE"
BASE COUNT          216 a          104 c          106 g          199 t
ORIGIN      
        1 aaagcctttg tcggtgattt agaaaagctc tataaagata tgcttaatct tacttaccgt
       61 actgaagatg aggattctat tcgctacttt gttttgttta ctggttttga aatcaataaa
      121 acaaaaaagt ttgttgaagt cagtgttaat ccggacctag aatatattat taatggttta
      181 acagctgaat ttagtagatt tgaattatct gctttcacgt cgttgcgttc tacgtatgca
      241 aaaactctat tccgactgct tatgcagtac cgttctactg gttattatgt agttcgtatt
      301 gaagagttta ggagcttaat ggatatccca gaatactatc aaatgggtaa cattgatcaa
      361 aaagtattaa atcctgcatt acgagaacta aaaaaatact tcaatgatct taaagtcaca
      421 aaaataaaag caaaaaaggg caataaaatc gccaaattag aatttacttt ttcaagccta
      481 acgtcagatt tgccaaagat ttctctgcgt aactggttag aagaataaat aaaaaagaac
      541 tcgcccccct tacattaagc aagtagacaa gtcccttaca accgactaag attgctagta
      601 cattctagca tattgtatgg ggctt
//
LOCUS       DAZKNO010000327          614 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000327 DAZKNO010000000
VERSION     DAZKNO010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.347"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            375..>614
                     /locus_tag="ACG8MY_002791"
     CDS             375..>614
                     /locus_tag="ACG8MY_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0431890.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRT"
BASE COUNT          192 a          112 c          118 g          192 t
ORIGIN      
        1 tgttataata gttttgctgt ctgctacccc ggcctaatct gtcaagggga ggaggagata
       61 taatgaaata cctttttgat tttgtttttg caatcaagcc atttgcagat gtagtgtgca
      121 gttacctctg caaatggctt gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt
      181 aattctataa tatagaaacg taaaaaaacc ccatcagcct gcacgctgat ggggtttggt
      241 gatataatga aaggctctat actttttgat gttggtgaac ttcaccaaca tcttttttgt
      301 ataaaaaaat atagaaaaga caccctgaaa tcctttagaa ttaatttggc taccaattca
      361 taggaggatt caagatgtct catactcagc ttatcaaaga taccctaaat attctagacc
      421 taaatatcta tttcgaggaa aattgtttaa ctaaagaaaa atacaaaggg caaatttgct
      481 tgatttacaa aggaactctt ctgtacaagc cagaagagtg ttcccattgt ctttgtgtcg
      541 tcccctctag gatcattcgt tgggggacga caaccgttcg gttgttactg aatgatgtgt
      601 ctgagtatcg cact
//
LOCUS       DAZKNO010000328          610 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000328 DAZKNO010000000
VERSION     DAZKNO010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.348"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..473)
                     /locus_tag="ACG8MY_002792"
     CDS             complement(<1..473)
                     /locus_tag="ACG8MY_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFQ0431891.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPT"
BASE COUNT          176 a          116 c          117 g          201 t
ORIGIN      
        1 gtcggaagga agttcatacg tgactggtaa tgcttcgttg cttcacgcaa gatccgtgtc
       61 accgtcccat ccgagacgaa gtacttctta gcgattagtt tacgtgattg attttcgtac
      121 agttccatga gaattgcctg cttcagttcc tttgttagtt gatgatgttc atcaactaag
      181 tccgttttcg cagaaaaggt ccttccacaa tcatggcaga gaaaacgggt acgttttaat
      241 tctaaaatag ttaacttttc tttgactttc aacatctgag tgcgtgtcgt ataggaaccg
      301 ttgcggataa tacgaaccga tcgacagtgt tcacaatagg aaggagagtc atccaatttt
      361 cctttaataa actgcgcttg aacgccttta tattcttttt caatcagcca ttcttcatca
      421 aaaaataaat gtttatctgt taatccgagt aattttctag tatgattatc cattgagaga
      481 ctcctttggt aggatgacgt tttggtcgac taaattttac caaaagtgag tctctttttg
      541 agttgaaacg aagaaaaagt gttagtagaa aatcgccttc gattttccac caacacaaaa
      601 tattatagaa
//
LOCUS       DAZKNO010000329          608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000329 DAZKNO010000000
VERSION     DAZKNO010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.349"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..338
                     /locus_tag="ACG8MY_002793"
                     /pseudo
     CDS             52..338
                     /locus_tag="ACG8MY_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          212 a          105 c           75 g          216 t
ORIGIN      
        1 aataaatact ttaaatctat tgactacaag tagtcattaa gtcttcataa gatgtacaat
       61 atgacattaa aaaaataaaa gcttacgacc atccgttaaa tctgacacta aataatggac
      121 tgctttcttt tgtattagca ggttacgttg actatgaata ttcaccagat tatttggaga
      181 aacctcttca acacgagaac aatgatatta attttagatt tgtctacgag tcaaaaagta
      241 aaacatggtt agttgatcaa ttaagcacga taaacacaag cttccatgaa ttacctaaac
      301 ctgaaaatga agttgagatg gtcattaaaa tcttctaaaa aagaagcgtt gaaggggaaa
      361 tattcctttc aacgctcttt tttatatgct tagaaacata aataaattgt atactttctt
      421 tttagcccct attacttttc acgaaaaata tctttttttc gacgctagcg tttctctact
      481 tctaatactg tacacagttt ttgtattatt gaaaaattac tcattattta gcattaatcc
      541 acatcgtttt atcaatcgac cttcatacta aactacttta aagtcatctt ccttctcgtt
      601 tttcatta
//
LOCUS       DAZKNO010000330          608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000330 DAZKNO010000000
VERSION     DAZKNO010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.350"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..511)
                     /locus_tag="ACG8MY_002794"
     CDS             complement(134..511)
                     /locus_tag="ACG8MY_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431892.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          186 a          106 c          108 g          208 t
ORIGIN      
        1 accaaaaaag aagcagatcc gttagcggtt ctgcttcttt tagtttatta tattttctta
       61 tcaataatag gaacctgatt ttaaaagcgg ctacctcttt accgtgcctc tttacataca
      121 aaaaatagag gaattaagcg taaatggctt ggctattata actaaaatgc tggttatgga
      181 gatgttgcag caagttttcg agaaaagcgt attccatctc ttgtccattg aataagatct
      241 ggatacgata atgatttttg aaacgcttct gttcatgaat ctgactgatt gaaagttcgc
      301 ctttgtgttc tgtctgataa agaaaagtcc ttttacggca ttcagcacag cgcttggttg
      361 ctcggcggat ttttcggaat gtataaaaac tagtatcttt attgcataaa taaacgtctt
      421 tgatcgacag caaatcatta tatagacagg aacgttcttc agctgttaat tctgataaaa
      481 aaagctgacc ttgttttatt gcatcttcca tcggcaagtg cctcctcgaa cccattatcc
      541 aaatttttag ttaattctaa tataccatca ttaagtataa atgtaaacgc ttgttgatgg
      601 tataagat
//
LOCUS       DAZKNO010000331          604 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000331 DAZKNO010000000
VERSION     DAZKNO010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.351"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..323
                     /locus_tag="ACG8MY_002795"
     CDS             <1..323
                     /locus_tag="ACG8MY_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0431893.1"
                     /translation="EALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIY
                     STNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTL
                     ESMI"
BASE COUNT          216 a          106 c           75 g          207 t
ORIGIN      
        1 aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag tatccaagag
       61 tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct cacgccatca
      121 gacgaacaat ttattcgacg aacctgattg aaggctttaa taagcagcta aaacgatata
      181 ctcgaagaaa agaacaattc cctaatgaag aatctctaga gagattcttc atttctcaat
      241 ttaatcaata taaccaaaaa tttttaggta gaattcacaa gggatttaaa gaaattcagg
      301 atacattaga gtcgatgatt taactgtaat taacagaatg gattttccat ttacatataa
      361 ttcttgacgc taccataatc taaataacca tcatattcat ctactactta tataattgag
      421 ttttattaaa ctttttaagt ttctattcta tcaaatattt aaacaatctc tgcatccgaa
      481 aagactacct cgtatagttt tactaacaat atttttatta gcgtgttcaa atcctgattt
      541 cttgtatcaa ttattttctg ttcactttca ataagaaaaa agtctttact tccatggata
      601 ataa
//
LOCUS       DAZKNO010000332          601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000332 DAZKNO010000000
VERSION     DAZKNO010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.352"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>601
                     /locus_tag="ACG8MY_002796"
     CDS             <1..>601
                     /locus_tag="ACG8MY_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFQ0431894.1"
                     /translation="RKTIKYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKK
                     DSESITYLLSGNRYSSKKYVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINV
                     LFAPTFRNNIEDNGITQLEWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSI
                     EYMKKHQITLINDGIPLEMLFEEIDVLITDYSSIFFDFAL"
BASE COUNT          231 a           82 c           95 g          193 t
ORIGIN      
        1 gtcggaaaac gattaagtat gcaaaaagga aaaaacaact ttattttcaa acatggcacg
       61 gaacgccttt aaaaaaaatt gaatttgatg caaaagataa gttacctaaa cgttacctaa
      121 gctatgcgaa aaaagatagt gagtcaatta catatttatt atctggtaat cgctatagtt
      181 ctaagaagta tgtttcagca ttcaatatcg ttccttctaa aatattagaa gtaggaactc
      241 ctagaaacga tgaattagcc tctagtaaag agcaagtgtt tgacttgaaa aagaaacaaa
      301 tcaatgtctt atttgctcca acttttcgaa ataatattga ggacaacgga attacgcaac
      361 tagaatggtt aggtgtggat aacttacgtg aattttttaa aaagcaacaa caagaattaa
      421 attttatgac taaatttcat cctaatgttc atagtaaatt agcaactgat tctaaaagta
      481 tagaatatat gaaaaaacat caaattactt taattaatga tgggattcct cttgaaatgc
      541 tttttgaaga gatagatgtt ttaatcacag attattcatc tatttttttc gattttgcgc
      601 t
//
LOCUS       DAZKNO010000333          595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000333 DAZKNO010000000
VERSION     DAZKNO010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.353"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..357)
                     /locus_tag="ACG8MY_002797"
     CDS             complement(<1..357)
                     /locus_tag="ACG8MY_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0431895.1"
                     /translation="MHLHRISALAVQIGRYHHSETRAVMEGLEEAIQTTNDCPYRRTF
                     HSDQGWAYQMKAYRNKLKEYKIFQSLSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNE"
BASE COUNT          173 a          113 c          126 g          183 t
ORIGIN      
        1 ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct cgaaacagtt
       61 ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct ccataagtga
      121 attatctaag cagttccctt tccgagacag gctttgaaag attttgtact ccttcaattt
      181 gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg tacgacgata
      241 gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg cacgtgtttc
      301 tgaatggtgg tagcgaccga tttgaacggc tagggcggaa atacggtgca aatgcacggt
      361 ggtttcatgc aatgcttaca gggcgtattt tgtaagggta tgtaacggag atgatcccga
      421 cggtaatgga tgtgagaggc caagccgacg ctgacggtag agcaaacaaa catagttgca
      481 ctctagcgat cgttatttat ttttaacggt caaactaggg tacaactatg cttaaaacac
      541 ttcgcctcaa cttctggtag caaaagtgat tttgaaagtc aaaatatttt ttatg
//
LOCUS       DAZKNO010000334          594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000334 DAZKNO010000000
VERSION     DAZKNO010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.354"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>594
                     /locus_tag="ACG8MY_002798"
     CDS             <1..>594
                     /locus_tag="ACG8MY_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0431896.1"
                     /translation="VSNIYHNCVPDKIKNRRNTDQLKQRDTVIIACVIWGIINGYTSQ
                     RATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFP
                     SPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHD
                     VKVLETLSEEANLPNILGDKGYISHKIHEKLALKGIT"
BASE COUNT          202 a          110 c          104 g          178 t
ORIGIN      
        1 tgtttctaac atataccaca attgtgtacc agataagatt aaaaaccgaa gaaatactga
       61 tcaactgaag caacgcgata cagtgattat tgcttgtgtt atatggggca taattaatgg
      121 ctatactagc caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga
      181 ctttcctagt aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat
      241 tattcgatac tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag
      301 ttttcctagt ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat
      361 agccaaagtt ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat
      421 gatcgttact aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga
      481 cgtgaaagtg ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa
      541 aggatatatt agccataaaa tccatgaaaa gcttgccctt aagggcatta ctat
//
LOCUS       DAZKNO010000335          592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000335 DAZKNO010000000
VERSION     DAZKNO010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.355"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..449)
                     /locus_tag="ACG8MY_002799"
     CDS             complement(42..449)
                     /locus_tag="ACG8MY_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431897.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     KNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(468..>592)
                     /locus_tag="ACG8MY_002800"
     CDS             complement(468..>592)
                     /locus_tag="ACG8MY_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0431898.1"
                     /translation="IGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          161 a           99 c           77 g          255 t
ORIGIN      
        1 agtataactt atactttaat tgaataccgc atgaaaaaaa attattttgg taatttttca
       61 aactcttctt gtatttcttc tcttatgtgt tcttcttttg ctactcctag tctatgctgg
      121 atatatgagt ctgctaatgt attaacaatt ttttcgaaag aaattctccc tttttcactt
      181 tgtaactccg tcatatagtc ttgtaaaatt gagagtttgc tatttaatgc cggagagatc
      241 tttttaggaa ctgatacctt gtttctttta tcaggaattg aaactggtcg gcctcttttt
      301 ttcaatgtaa tattttttgt gttactcaat gcactattgc taaagttata ttcaccgctt
      361 attttcacat cttggcgttt gttattgggt aataatggct caaattcgcc attctccatc
      421 attttaggaa tatcaaaatc tttaggcatt acttatgcaa ctccttatta tttatttaaa
      481 aactcttgtc tagcatctag ttctgccaaa atatttataa atacttggtg tgctttataa
      541 tcccattgat caaaatttcc atttttgtat tttcttaaat gtataccaat tt
//
LOCUS       DAZKNO010000336          574 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000336 DAZKNO010000000
VERSION     DAZKNO010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.357"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>574
                     /locus_tag="ACG8MY_002801"
     CDS             <1..>574
                     /locus_tag="ACG8MY_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0431899.1"
                     /translation="NKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTRE
                     LQYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGC
                     YRSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIE
                     SGIHEKDICVLAPQHYPLFSLGEYLKKELPY"
BASE COUNT          216 a           79 c          102 g          177 t
ORIGIN      
        1 aaataaagaa attgattttg atatgatttt actagtagcg tataaaattt taactgtaaa
       61 cagtaacgtt gcacgtaata ttgctagtaa tataagggcg atttatgtgg atgaatttca
      121 agatactaga gaattacaat acaatgtaat agctaaatta cttcaaaaca accctcaaat
      181 tcagtcaatg tttgttggag atattgacca agcaatttat ggatcattag atggtatagc
      241 taaatcagtt gaagaactag aaatgctaac tagtcacact tttcaatcaa agacgctaca
      301 tggctgttac aggtctaatc agagattagt agacttctac tctaattttc agagttgtcc
      361 ttacgagatt gaaagcagag ggaataataa aaacgaccgt ggttttataa gttatgattg
      421 caaagttact aaggaggatt tacccaacat aataaaaaat ataataaagg aaaagattga
      481 aagcggcatt catgaaaaag atatttgtgt acttgctcct caacactatc ctctgttttc
      541 actgggtgag tatttaaaga aagaattacc gtat
//
LOCUS       DAZKNO010000337          555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000337 DAZKNO010000000
VERSION     DAZKNO010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.358"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>555
                     /locus_tag="ACG8MY_002802"
     rRNA            <1..>555
                     /locus_tag="ACG8MY_002802"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          177 a          108 c          162 g          108 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgta
//
LOCUS       DAZKNO010000338          554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000338 DAZKNO010000000
VERSION     DAZKNO010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.359"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..>554
                     /locus_tag="ACG8MY_002803"
     CDS             2..>554
                     /locus_tag="ACG8MY_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0431900.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDL"
BASE COUNT          205 a           92 c           93 g          164 t
ORIGIN      
        1 catggatact tttcaaggga ctacatttac agttggcaat tttaaaggag gagtaggtaa
       61 aactaagata gtcagcatgc ttgcttatga caatgcaatg ataagaaata gaaaaacgct
      121 tattattgat ttagaccctc aagcgaactt aacacaagtc atagcaagaa ctttcggtat
      181 aaccgatatc aaaaccacta taacaaatgg tgtcgcaaat ggagaattag caagtagtat
      241 tattaaggta aatgaaaatc ttgaccttct ggcatgtgac actagttttc gatcattctc
      301 cgcttttgct aataaacatg aaaatgaatt agaccaagtt actgtcttga aaaaattact
      361 tcttccaata aagaacaatt atcaagaaat atttattgat gtaccaccaa ccattagcga
      421 attttccgat aatgctatgg cagcttcaga ctattcgata atttcatttc aaacagctga
      481 agaatcctta gagggagtca acaaatatgt aaattatcaa aaattcatgg tcgatagata
      541 cgatcttgat ttgc
//
LOCUS       DAZKNO010000339          553 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000339 DAZKNO010000000
VERSION     DAZKNO010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.360"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..449
                     /locus_tag="ACG8MY_002804"
     CDS             <1..449
                     /locus_tag="ACG8MY_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0431901.1"
                     /translation="INLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPV
                     VQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIG
                     RYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          181 a          127 c          107 g          138 t
ORIGIN      
        1 atattaacct gatctactca ggacaacaat taacaatccc aacagaaggc tctgcacaag
       61 cagctgctga acctcaagca gccgttcaag aagcaccagt ccaagctgaa cctgttcaag
      121 ctgaacaacc tgttgtacaa gaaacggttc aaacggaaac acaagcagcg cctgttgctg
      181 aaacacaacc tgctcctgct gtaacagaaa ctgctgctac tccagcaagt acaagctctg
      241 caaaagaatg gatcgcacaa aaagaatcaa gcggttctta cacagcaacg aacggacgtt
      301 acatcggccg ttaccaatta gattcttctt acttaaatgg tgactactct gctgcaaacc
      361 aagaaagagt agcagaacaa tatgttactt cacgttatgg ttcttgggaa gcggcgaaag
      421 cattctggga agcaaacggt tggtactaag attcattatt tgaacgttta aacacattca
      481 aaatatagta agaggtcatg cgatttattc tttcgcatga cctctttttg agtttaagtt
      541 tagtctttat tcc
//
LOCUS       DAZKNO010000340          552 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000340 DAZKNO010000000
VERSION     DAZKNO010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.361"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..483)
                     /locus_tag="ACG8MY_002805"
     CDS             complement(<1..483)
                     /locus_tag="ACG8MY_002805"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0431902.1"
                     /translation="MYWYLFIVDNSRDPKNLKDSVLIWLRYFKASSKFRRLFFLAFYF
                     TLILFKTLLNRNMWMNPLSNVIGQWSLYIIDNATGESRLTTECVENTMLFIPFCMLLL
                     WWRDIKCDIIRTIYVGIKYVFLFSLSIEFTQLFFRLGTFQLSDLFYNTLGGLIGALLY
                     W"
BASE COUNT          214 a          102 c           80 g          156 t
ORIGIN      
        1 ccaatatagc aaagcaccaa ttaagccccc tagggtgtta taaaacaaat ccgatagttg
       61 aaatgtacca agcctaaaaa acagttgtgt aaattctata cttaatgaga ataaaaacac
      121 atacttaatc ccaacataga tggtgcgtat aatatcacat ttaatgtcac gccaccataa
      181 caataacatg caaaaaggga taaacagcat cgtgttctcc acgcattctg ttgtcaatct
      241 gctctctcct gtagcattat ctataatata taatgaccac tgtccaataa cgtttgaaag
      301 cgggttcatc cacatatttc gattaagcaa ggttttaaat aatatcaaag taaaataaaa
      361 cgcaagaaaa aagagtctgc gaaatttact agatgcttta aaatatctca accagattaa
      421 tacagaatct tttaagtttt taggatctcg agagttatca actataaata gataccagta
      481 cataaacaga tacgctgtta atacggcaaa taagaacggc tgatataatg ctgttaatac
      541 atttaccgtt at
//
LOCUS       DAZKNO010000341          544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000341 DAZKNO010000000
VERSION     DAZKNO010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.362"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          170 a           98 c           73 g          203 t
ORIGIN      
        1 aaattttcaa aagttgttct aaaaaatttt tcttttttgg aagttactct aacttttttt
       61 gcttgtggtg cttggtatgt tttctccgcc tcccaggttc ttgaccattt tctctcaacg
      121 tggtaaaaat cccacttata agagaaaatg gtcaagaacc tgggaggcgg agaatgccct
      181 taataccaag caccgcaagc ctttcccata ctttattcga tgatcccacg atgatctcat
      241 aatgattcta caatgaccct acgatgatca aaattctcaa aaaaaataaa cgaaatttca
      301 aacgattttt ttgaaatttt gttaaaatca catttgtttt tttatgactt tttcaacaaa
      361 atcgattaga atttattttg tttttccacc aaaaattgag tgagatttca acctatttta
      421 agcaataagt caccatatag tttgatagtt tacaactata aacattgatt gtttacagtt
      481 gtaaactatt ttttctttct cataaaaatt tgagtgatct ccccatcagg tagtacaact
      541 tttt
//
LOCUS       DAZKNO010000342          544 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000342 DAZKNO010000000
VERSION     DAZKNO010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.363"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..305
                     /locus_tag="ACG8MY_002806"
                     /pseudo
     CDS             1..305
                     /locus_tag="ACG8MY_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            380..>544
                     /locus_tag="ACG8MY_002807"
     CDS             380..>544
                     /locus_tag="ACG8MY_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0431903.1"
                     /translation="MSKRQRRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
BASE COUNT          185 a           96 c          111 g          152 t
ORIGIN      
        1 ggaaaaagtg gcattatatc tgcttaatac ttgatttgtt taatcgagaa attattggtt
       61 attcctgtgg tgagaagaaa gatgcctcat tggtaaaaga agcctttgga cggataccgt
      121 attctttaac agacgtcaag ctttttcata cagaccgtgg aaaggaattt gataaccaaa
      181 ccattcatga gattctgaat ggttttggaa ttactcgttc attgagtagg aagggttgtc
      241 cgtatgataa tgccgttgcg gaatcaaccc ataaatctgt caaagtagaa ttcgtgcatc
      301 aatactgaat cggctacact agactagaca gaaaaaataa ggtgtgtaga ataataaaaa
      361 acacactgga ggatttttca tgtcaaaacg tcaacgaaga acctattcaa aagaattcaa
      421 acaacaaatc gtcgatctct atctcgctgg taagcctcgc gcagaaatta ttcgagagta
      481 tgagcttacg ccttcttctt tcgataaatg gatgaagcaa gcacaatcaa cgggctcatt
      541 caaa
//
LOCUS       DAZKNO010000343          535 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000343 DAZKNO010000000
VERSION     DAZKNO010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.364"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG8MY_002808"
     CDS             <1..227
                     /locus_tag="ACG8MY_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431904.1"
                     /translation="KDTKIEEACSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQ
                     ATNMQNPTKKETALNYLKSVKEWLDQQQQN"
     gene            complement(348..434)
                     /locus_tag="ACG8MY_002809"
     CDS             complement(348..434)
                     /locus_tag="ACG8MY_002809"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0431905.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
BASE COUNT          201 a          100 c           96 g          138 t
ORIGIN      
        1 taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca aacccttggt
       61 ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa cgctacgaaa
      121 acctgtttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc gccttaaact
      181 atctaaaaag tgtcaaggaa tggttagatc agcaacagca aaattgaatt tgattgaaac
      241 tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc ccagagaggt
      301 cggattctct ggggcttttt ttcaggttga tgtcgccttt actgatttta cttcttgttt
      361 cggttattca accaataact gaataaagag acacaaactc ctacaagaag ggggcaaatg
      421 aactgttcaa acattagata cacctccttc cagtcgcaag actgtcggat aggcgacaag
      481 ttattatacc ctacaaaatt aaatctgtgt ggcgatttta agtatacctt atttt
//
LOCUS       DAZKNO010000344          533 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000344 DAZKNO010000000
VERSION     DAZKNO010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.365"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..297
                     /locus_tag="ACG8MY_002810"
     CDS             103..297
                     /locus_tag="ACG8MY_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431906.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          224 a           66 c           87 g          156 t
ORIGIN      
        1 aaattgattc tttttattta tcataaagat atagatacat aatcattttc acttacatat
       61 atttgaatat taactaaaaa cagcaagaaa gaagaggatc aaatgtatta tgtagaagta
      121 aaaacaaaag gcgtcaaaaa caaacaatat gtgaaaggta tcagtaacga atatccttta
      181 cttggttcat ggaaagaagc tgctcctttt tccaaaccat gtgccataaa aataaagaac
      241 gagctagaaa aagaactgac ttgtggtaaa gctgttgtag atatcatcga aaagtaaatc
      301 caatgtatag aaaggaagat cacacatgaa aagcgacgta caaattgcta agattataga
      361 attagaagaa gaagacgcag tgttgacaat tgaaaaaaaa taaatgtttg tataccatat
      421 gattatttca atatgtatcc tggaattgga gatctcgtag agctatatca agatgaatca
      481 aatatacttg tcattcatca tgaaatatag agacttataa aattgtttat ttt
//
LOCUS       DAZKNO010000345          518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000345 DAZKNO010000000
VERSION     DAZKNO010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.366"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..>518
                     /locus_tag="ACG8MY_002811"
     CDS             62..>518
                     /locus_tag="ACG8MY_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0431907.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYA"
BASE COUNT          185 a           84 c          109 g          140 t
ORIGIN      
        1 aacaaaatga tatcgttata ttaaaaccgg catcggttac attaaaattt ggaggtaaaa
       61 tatgaaaaaa ttattgttta cagggttgct ggcaagttca gcattgtttt tattaggagg
      121 atgcggatca agtaatgcaa gcgatgccag ttcaagtgca gcaaattctt ctgaaaaaac
      181 aagtgatgga agaaaaaata ttgatttctg gtccttttgg ggatccggtg cacgaaaaga
      241 agctatcgaa gaaatcattg aggatttcaa tcaatcacaa aacgagatcc atgtaaatta
      301 taagtatcag ccatggggtg acatctggac taaatcttta tctgcaataa cagctggaaa
      361 tgcacccgat gttattgtac aggacataaa ttccgttgcc caaagagcag aagcacagca
      421 agcaactaat ctctccaaat atgtagaaaa agacacttct aaggattttt atccgcagct
      481 ttgggataca gtagaatatg aaggtgatcc ttatgcga
//
LOCUS       DAZKNO010000346          501 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000346 DAZKNO010000000
VERSION     DAZKNO010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.369"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..323)
                     /locus_tag="ACG8MY_002812"
     CDS             complement(<1..323)
                     /locus_tag="ACG8MY_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFQ0431908.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQE"
BASE COUNT          144 a           96 c           73 g          188 t
ORIGIN      
        1 ttttcctgaa agaaagccaa taccattttc atagataaag aatccgttat ataacgagat
       61 aacagagctt tgctagaaca ttttccatta atttccagac atatatccaa ttcactgacg
      121 gttactcgca aatttttctc tagatcgaac ttttgtaaat catgtttcag cttatcgatc
      181 gtttttttca agagaaatgt cgatagcgac aactcttctt gaacattcga tattgactct
      241 gataaatttg agcttgcttc taaatattta agtaatgaat aacttctgtg atcttctttg
      301 cttaaaaaaa actcataatt cattagtcat cagctctttc tacgtatatt actttccatg
      361 cattcaccaa ctagctgggg ataaaactga tttcctggat ccgagtcact gttgtcagcg
      421 attcttgttt gtataatata catcgtcctg tctttttttt aacattttgg agagcgtttt
      481 atttggcctt tctcgcgatc a
//
LOCUS       DAZKNO010000347          495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000347 DAZKNO010000000
VERSION     DAZKNO010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.370"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..>495
                     /locus_tag="ACG8MY_002813"
     CDS             72..>495
                     /locus_tag="ACG8MY_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431909.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINA"
BASE COUNT          180 a           79 c           67 g          169 t
ORIGIN      
        1 ttgtctaaat ttatgaaaaa aacagtaata ttttcaagtt ttaacaattt aagtaaaaag
       61 gagcctcttt catgcaaact caagaagcga ttaaaacgtt tatcttgaag aaaaaaagca
      121 atacaaaatt agatattttt cttttcctat ctgaacatcg tttctttatt actacacaat
      181 atttagcaga tcatttccat atgtctgaat caaatttttt actatacatc aaagaattag
      241 aacaagactt tgagcgtctc aatctaacag aactgcatat tgataagcaa aaaccttttt
      301 taaagttgaa ttttgaagga attgatccgg cttactgcta ttatcgtcta tttggaagat
      361 actgcaacga atcagtcagt tatcagatct tgacttcttt gttcagctgt cagacaaatt
      421 cgattatcag tttcagtcag caaacaaact atagtgcctc ttatttgtat acgaaaatga
      481 aaaagattaa tgcat
//
LOCUS       DAZKNO010000348          482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000348 DAZKNO010000000
VERSION     DAZKNO010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.371"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..143)
                     /locus_tag="ACG8MY_002814"
     CDS             complement(<1..143)
                     /locus_tag="ACG8MY_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0431910.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQ"
     gene            complement(154..360)
                     /locus_tag="ACG8MY_002815"
     CDS             complement(154..360)
                     /locus_tag="ACG8MY_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0431911.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          137 a          107 c           66 g          172 t
ORIGIN      
        1 tactgattat ttgctacttg tgagctacct tcatttgcgc ccaaattaaa ttcattgttg
       61 atagtatagg caaatccgtt aattggcaat aaaaaaagag ccgttaacag gctcatcgca
      121 gtaatagtaa ttttcttttt catatctatc ctcctattta tctgatgaaa ttttcttata
      181 cgttcgattt gatacaccta aaatactccc tagaaatgct ccaaaacctg taataatcat
      241 caccgttata tctgtgtacc gccagttaac accttttccg acaagaccga caaaagtcgc
      301 taaagcgggg ataactacta tagcgatcca tttcaaaata tcaaaggttc tattgttcat
      361 actatccacc tcccttcatt ggttgggcta ctttcttttt caatcgcttg ttttcttcta
      421 tcaatatttc tctttctgaa agcaaggcat caatttgccc gtccagttgt tcaattttat
      481 ct
//
LOCUS       DAZKNO010000349          479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000349 DAZKNO010000000
VERSION     DAZKNO010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.372"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>479
                     /locus_tag="ACG8MY_002816"
     CDS             <1..>479
                     /locus_tag="ACG8MY_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0431912.1"
                     /translation="EIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRT
                     GFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLF
                     EKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          174 a           87 c           80 g          138 t
ORIGIN      
        1 gaaattgtgc aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta
       61 gaaaaagcga taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa
      121 tatgatcgca ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc
      181 aaaaccgaat atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa
      241 caacaaactt taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat
      301 ttattcgaaa aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt
      361 catcactata ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat
      421 gcctttaaat ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DAZKNO010000350          469 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000350 DAZKNO010000000
VERSION     DAZKNO010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.374"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..256
                     /locus_tag="ACG8MY_002817"
     CDS             83..256
                     /locus_tag="ACG8MY_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431913.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
BASE COUNT          196 a           67 c           76 g          130 t
ORIGIN      
        1 atttaaacat ttgataaact gaaggctagt atttaaaatg tgtatacaag tggtagcgtt
       61 aaaaaaagtg gaggtataac ctatgttaaa aaaaactgtg ttggtactgg gttctatttc
      121 tctaggaata ttaggtttta gccccatgct attatccgta attggaaaaa acaattccaa
      181 cagagaactt aaattgaatt caaattatta caacagtcct aacgaagaat tgtttgtaaa
      241 agaaacggat aaataataaa aaagactgct cttccgagca gccagttaaa aaataatgag
      301 tgtttattct tgaaaaagca acgcgcttat aaagcgctac caaaaattta tcatatttta
      361 tgaaaaataa caatccagta aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag
      421 caactgcaga aaatcaagcc ttaaaaagag ctttttacct agaccaatt
//
LOCUS       DAZKNO010000351          465 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000351 DAZKNO010000000
VERSION     DAZKNO010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.375"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(268..341)
                     /locus_tag="ACG8MY_002818"
     tRNA            complement(268..341)
                     /locus_tag="ACG8MY_002818"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(305..307),aa:Ile,seq:cat)
BASE COUNT          160 a           87 c           84 g          134 t
ORIGIN      
        1 acacacttat ggggaagtgg tttggggtgc gctttaaact cttctttatt attatctcac
       61 aatttaaccc aaatgtcttt ctatttaaaa atcaaagtaa aacttttcaa atatacagaa
      121 gtataactat gtgaaacatc ctttcattaa tccataaaag gatacataaa aaagccactc
      181 atttgagtgg caatgaagaa aagctttagc ttgtataata ctcttcaaaa aaattctaac
      241 acagaacgat tcaaatggct acgttaatgt accctgtagg actcgaacct acgaccggac
      301 ggttatgagc cgtctgctct gaccaactga gctaagggta ctggttgttg ccacataaag
      361 ccataaacaa tcaaccagta gaatgtgtgg caacaaacct gttatcgcat agcttggagt
      421 gtgactatta tgggtgatag tgaagatatg cgataacatc actat
//
LOCUS       DAZKNO010000352          464 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000352 DAZKNO010000000
VERSION     DAZKNO010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.376"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            59..>464
                     /locus_tag="ACG8MY_002819"
     CDS             59..>464
                     /locus_tag="ACG8MY_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0431914.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMAD"
BASE COUNT          159 a           61 c           88 g          156 t
ORIGIN      
        1 ccatattgtt tttggagtta ttatccaaaa ataataaaaa attggagcgt ttaggataat
       61 ggataaaaag aggtttttag atcttatttc aaaaggtttg attgtttcat gtcaagcatt
      121 acctggtgaa ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc
      181 tcaaagggct ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag
      241 aaagtgtgta tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc
      301 attcattact gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat
      361 cgctcttgat tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac
      421 taaaataaaa gaaaaatatc ctcatcagtt atttatggca gata
//
LOCUS       DAZKNO010000353          462 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000353 DAZKNO010000000
VERSION     DAZKNO010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.377"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG8MY_002820"
     CDS             complement(<1..430)
                     /locus_tag="ACG8MY_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0431915.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAZKNO010000354          459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000354 DAZKNO010000000
VERSION     DAZKNO010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.378"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..316
                     /locus_tag="ACG8MY_002821"
     CDS             20..316
                     /locus_tag="ACG8MY_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0431916.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
BASE COUNT          173 a           67 c           84 g          135 t
ORIGIN      
        1 cttctttgga ggaatcacta tgaaggaata tataaaaaac atctatttta tcgaagaaac
       61 acaaaatatc gagggttctt atattgaagt aaaaacactc tttgtcaatg aagataaaac
      121 caaagcattg gatatttata aaaaattagc ttcaaaaaaa acgaatagtt ttggattgat
      181 attaagcgag tataaaatca aagcagaaga gagttatttc tatcaattat taaaacggtg
      241 gagtaagctt cccgccgatt tttatcgaaa aatgcagatc atcaactatc aaccgcttgc
      301 tgagacacat gcctaaatag aggttgtgaa atcagtgctt tgagctaata ccgaaggtca
      361 gctgattgaa cattgtttag aagagattgt gacaactgcg atttgctttg agtaataaga
      421 acgctggaca gaaaatagct tctcatattt tctagtcta
//
LOCUS       DAZKNO010000355          449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000355 DAZKNO010000000
VERSION     DAZKNO010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.379"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..182
                     /locus_tag="ACG8MY_002822"
     CDS             <1..182
                     /locus_tag="ACG8MY_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HFQ0431917.1"
                     /translation="QLSELPIISVQDSYGLVDVFKNQQEKLLKSSQELLDYIGNYETG
                     ASTEKVLELIERDIK"
     gene            179..>449
                     /locus_tag="ACG8MY_002823"
     CDS             179..>449
                     /locus_tag="ACG8MY_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0431918.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFL"
BASE COUNT          157 a           54 c           79 g          159 t
ORIGIN      
        1 tacagttaag tgagttacca attataagcg tacaagatag ttatggcctt gttgatgttt
       61 ttaaaaacca acaagaaaag cttttgaaaa gttctcaaga gttacttgat tatattggaa
      121 attatgaaac aggagcaagt acagaaaagg ttcttgagtt gatagagagg gatataaagt
      181 gaaaaaaaca gtaaaagata tattttataa tgcgatatat caagtctttt tgattgtttt
      241 acccttactt actataccta tacttagtcg tagaattggt tcaactggtt tagggattta
      301 tgggtatgtt ttctcaatat cacagttttt aatgactgtc attgctgtgg gaatgaatcc
      361 ttttcgtatt cgaaatatcg ctaaatcaag gaaagataaa aaagcactat ctttacaatt
      421 ctggaatatt tactttattc aatttttaa
//
LOCUS       DAZKNO010000356          449 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000356 DAZKNO010000000
VERSION     DAZKNO010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.380"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..202
                     /gene="tnpB"
                     /locus_tag="ACG8MY_002824"
     CDS             <1..202
                     /gene="tnpB"
                     /locus_tag="ACG8MY_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0431919.1"
                     /translation="QDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVR
                     WLLEGLSIDQPRAILPGKKGVF"
BASE COUNT          152 a           81 c           90 g          126 t
ORIGIN      
        1 tcaagaccgt ttcaaacttc tctattggga cggtgatggt tttcttctct gttataagcg
       61 gatagaaaac ggcaagttaa aatggcctcg gacgaaagac gaagttcgaa ccttaacgaa
      121 ccaacaggtt cgatggttgt tagaaggatt aagtatcgat caacctcgag ctattttgcc
      181 aggaaaaaaa ggagtttttt aagctggggg gttaacacaa aaaatgtgtt gaacccctat
      241 ttcttgtgcg tttcttgtgg tattctgtaa caaaatcagt tcgattcaag gtggtgaaaa
      301 attttggaaa ctacagatac gttgcttcag ttgcttcaag aagcccataa aaccaatcaa
      361 gcacaacaac aaaccattca gaatcttaca actgaaatcc aactattgaa tgaaaaggtc
      421 aattatttaa ccaacaaatt atttggacg
//
LOCUS       DAZKNO010000357          445 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000357 DAZKNO010000000
VERSION     DAZKNO010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.382"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(207..407)
                     /locus_tag="ACG8MY_002825"
     CDS             complement(207..407)
                     /locus_tag="ACG8MY_002825"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431920.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
BASE COUNT          110 a           88 c           50 g          197 t
ORIGIN      
        1 agccatctct tttcctttta gaaatggtct ttctaatccg gtatcaattc ttgcttgatc
       61 aatagcctct tttgtcattt caaaaacata atttttcaat tgattcgttt gttgtttatc
      121 taacattact gtaattcctg catccattat ttttctcctc tcattttcta tttcttcaat
      181 cagccaactc gccaagttgt atttatttag attttcaaat cattaagtaa ccaatttacc
      241 aacttttgat aagtagatgc tctaatactt tctttgctcc cattttcgat tttgacatat
      301 gttttccgat ttatacctat caattttgct gcatcgtttt tagtaagtat aagatcggct
      361 ctatgtcttc ttaagcttct aatgaattga tcattaagag ttatcattca gcgcctcctt
      421 tcaaattatt cactttgtgg ttttt
//
LOCUS       DAZKNO010000358          431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000358 DAZKNO010000000
VERSION     DAZKNO010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.384"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..179)
                     /locus_tag="ACG8MY_002826"
     CDS             complement(<1..179)
                     /locus_tag="ACG8MY_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0431921.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITK"
     gene            complement(198..359)
                     /locus_tag="ACG8MY_002827"
     CDS             complement(198..359)
                     /locus_tag="ACG8MY_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0431922.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          143 a           76 c           47 g          165 t
ORIGIN      
        1 ttttttgtta ttgatattga agaattagaa ctttcatata aagccttttc tatttcattg
       61 cttgatttca ttattgttaa acaagaatac aagcaagaaa gaactatagt taatatgata
      121 aaaattatta aagttctatt tttctttctt gtaatatatg ctatagcatt ttttatcact
      181 ttcctaacta ctcctctcta ttttcttact ccataactaa ttgcatccac tggacaagca
      241 tttttacatc gtccacatct gatacactcc agatgattgc aattctcaac tgaatcttta
      301 ttcatttggc aaactttttt acattttcca caattaatac acttatcctt atcctgaatc
      361 ggctacacta gactagacag aaaaaataag gtgtgtagaa taataaaaaa cacactggag
      421 gatttttcat g
//
LOCUS       DAZKNO010000359          427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000359 DAZKNO010000000
VERSION     DAZKNO010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.385"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..411
                     /locus_tag="ACG8MY_002828"
     CDS             <1..411
                     /locus_tag="ACG8MY_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431923.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     ELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
BASE COUNT          132 a           75 c           83 g          137 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt taaaagggta ccatgttact accggtgaac ctgtttgcca gccccgtcgt
      361 ccaaatagtc ggatagataa agtacgatat atgttcaata aaataactta gtgtatttta
      421 tgttgtt
//
LOCUS       DAZKNO010000360          427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000360 DAZKNO010000000
VERSION     DAZKNO010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.386"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..426
                     /locus_tag="ACG8MY_002829"
     CDS             <1..426
                     /locus_tag="ACG8MY_002829"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0431924.1"
                     /translation="YSLKKYAGKPIIIVNTATKCGLAPQFKQLEELYQAYQTQGLVIL
                     GFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTDPLFTYLKNEAPGTVT
                     KAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          152 a           85 c           66 g          124 t
ORIGIN      
        1 tattcattga aaaaatatgc aggcaaaccg ataatcattg ttaacactgc aacaaaatgc
       61 ggtctagcac ctcaatttaa acaattagaa gaactttacc aagcttatca gacacaaggt
      121 ctggttatct taggctttcc ttccaatcaa tttaaacaag agctagattc agcgactgaa
      181 gcagcagaag cttgtaaaac cacttatggc gttaccttcc ctatgcatca attgattgac
      241 gtaaatggag aaaatacaga tcctcttttt acttatctta aaaatgaagc tcctggtact
      301 gtcactaaag caataaaatg gaattttact aaatttttga ttgatagaaa tggcaaagta
      361 atcgaacgat ttgcaccaca aacttctcca ttgaaaatga ccgctgcaat tgaatccgtt
      421 ctttaac
//
LOCUS       DAZKNO010000361          423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000361 DAZKNO010000000
VERSION     DAZKNO010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.387"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..423
                     /locus_tag="ACG8MY_002830"
                     /pseudo
     CDS             55..423
                     /locus_tag="ACG8MY_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          169 a           69 c           81 g          104 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atc
//
LOCUS       DAZKNO010000362          405 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000362 DAZKNO010000000
VERSION     DAZKNO010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.388"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            108..>405
                     /locus_tag="ACG8MY_002831"
     CDS             108..>405
                     /locus_tag="ACG8MY_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0431925.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFI"
BASE COUNT          159 a           47 c           76 g          123 t
ORIGIN      
        1 atagcaataa tttctttatt agagtacttt ggtaaagatg aggatgttgt gtatctaatc
       61 aatcagatag aaaattacac aaaaatactt tatgctgagg tttcaaaatg acaaatatct
      121 tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa agtgaagata
      181 acctttatgt aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca aggaaaattg
      241 cttaccaagc aattaaacac gatggatcac taaaaagaat aattgctatc acatacacta
      301 atagagctgc tgaagaaatt aaggaacgcc taagtttact tagtatagat gatgatatta
      361 atatttgggt ggggacaata catcagtttt gtcttgaatt tatta
//
LOCUS       DAZKNO010000363          394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000363 DAZKNO010000000
VERSION     DAZKNO010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.389"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          129 a           53 c           57 g          155 t
ORIGIN      
        1 aaagatattt tcaaaacatt ttttcttgat gtgatatcct tgtctttttt gtaatgtttt
       61 taacttattg cttttgcaaa atttatttac ctgttttctg gatcaacgat agtgtatatt
      121 atgctatcct tgtttggatt ttaggacgtt taaagaggca cttttttaca taacttaatc
      181 catagacctt ttaagcatgc tgtgagataa taataaagaa gagtttaaag cgcaccccaa
      241 accacttccc cataagtgta ttacgcttta aactctttta tatttgaatt cactaagaag
      301 cataccatat ttttgaaaaa aagtgagaaa aaaggcttat aattaggatt tagagtaatt
      361 aattagatgt ttattttata ggactgatac tata
//
LOCUS       DAZKNO010000364          390 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000364 DAZKNO010000000
VERSION     DAZKNO010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.390"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>390)
                     /locus_tag="ACG8MY_002832"
     CDS             complement(<1..>390)
                     /locus_tag="ACG8MY_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0431926.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRY"
BASE COUNT          124 a           76 c           68 g          122 t
ORIGIN      
        1 atatcttact ttatctatcc gactatttag acgacgggtc tggcaaacag gttcgccagt
       61 ggtaacctga tatcctttta gctctgctaa acaaacacta agcccatttg taaaaaaagt
      121 taaatcattg cgataatctt gaatacatcg agcaggaatt tctccaataa taatgacctc
      181 attatttttc agttgagtat ttacgatatt tgcacaatat ttgggagcat cgttatatgc
      241 ccgtgaaaga tattcctgtg gtgcataaac tttaaaacta agatatggct ctaacaattc
      301 tgttccagct tttctaaagg cttgctccag tacaatagga gtaagcatcc gaaaatctgc
      361 tggagtacta acagggctat agtataaacc
//
LOCUS       DAZKNO010000365          384 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000365 DAZKNO010000000
VERSION     DAZKNO010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.392"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            118..>384
                     /locus_tag="ACG8MY_002833"
     CDS             118..>384
                     /locus_tag="ACG8MY_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0431927.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWM"
BASE COUNT          155 a           57 c           69 g          103 t
ORIGIN      
        1 acttattgtt taagagtgat acgcgaattg taaaattaag ttagaaaaat aaaaaggcat
       61 ttatggtaca ctcaaattgt atttccgacg aaagaaaaca aaggagtgta accataaatg
      121 acctacaaac atcttaccat agacgaactg acaatgatag aatcatatta tcttcaacat
      181 aataagccgg ttgaaatcgc taaccgaatg ggacgtgcta tacaaactat ttataatgta
      241 gtcaataagt tcaagcaagg caagactgct cttgattatt ggcaccagta taaagaaaat
      301 aagaaaaaat gtggtagaaa agtcattcaa ttacctgctc atgaagtaga ttacattaaa
      361 gagaaagtca ctcttggttg gatg
//
LOCUS       DAZKNO010000366          383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000366 DAZKNO010000000
VERSION     DAZKNO010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.393"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..209)
                     /locus_tag="ACG8MY_002834"
     CDS             complement(<1..209)
                     /locus_tag="ACG8MY_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0431928.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
BASE COUNT          121 a           74 c           46 g          142 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tct
//
LOCUS       DAZKNO010000367          378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000367 DAZKNO010000000
VERSION     DAZKNO010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.394"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          126 a           63 c           60 g          129 t
ORIGIN      
        1 tcaatagaac accaagagcc caacagggta tactctggtg tccttttaaa aaagtaactt
       61 tctgacctaa ttaattgagc tactaaaaaa gctatgaaat gaggaataaa aaagggatca
      121 aaaactttta agtttctgac ccttttgagt tctaattatt tcttccagaa cgaagaatgt
      181 tatctgcttt atctgcaatt gattctgtta gaaggactga aaaatttatg ccagcccttt
      241 tgcctagctt atccgcccat tgaggaattg ttaaggtttt tctgacaagt tctatgtctt
      301 tcaaatattt ggcaacatct acacagatta aaagcatgat tttgtatcat agtctattat
      361 aagagattta acaatttt
//
LOCUS       DAZKNO010000368          376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000368 DAZKNO010000000
VERSION     DAZKNO010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.395"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..>376
                     /locus_tag="ACG8MY_002835"
     CDS             91..>376
                     /locus_tag="ACG8MY_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0431929.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGY"
BASE COUNT          108 a           69 c           73 g          126 t
ORIGIN      
        1 agactgtaga caaagtccat tttatggatg atgtctacag tcttttcttt tataatagat
       61 ttatgaaaac gaatgcaagg agtgatagct atgatgtcta aaaacaatac gaatggtaga
      121 aatcaatttg cgatgcttac gattgatgat ctagttcctc aagatcatct cgttcgaaag
      181 attgatgccg cgcttgattt tgaatttatt tatccaatcg tcgaagccac ttactccgat
      241 ttaggtcgcc caagtatcga tccagtgatt ctgatcaaat tggtgtttat tcagtattta
      301 ttcggtatcc gttccatgcg acaaaccatt aaggaagtcg ataccaatgt cgcttaccgc
      361 tggtttttag gctact
//
LOCUS       DAZKNO010000369          376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000369 DAZKNO010000000
VERSION     DAZKNO010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.396"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..276
                     /locus_tag="ACG8MY_002836"
     CDS             <1..276
                     /locus_tag="ACG8MY_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0431930.1"
                     /translation="ANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGY
                     HVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          135 a           62 c           67 g          112 t
ORIGIN      
        1 gctaactata aatacgagta tatcttcatt atggattaca atactgggaa agaattagct
       61 cgagtaagag ctgatggaat ttatagatca gatgtaaatc aagcttataa tacttctgga
      121 aatgttggct atcatgtatc ttttaacatg cgtaacttcc ctaataagaa agtctatgtc
      181 atgatgcggg caacgaatga tccagaggga aacactaaag gcggtgcgca agatttccat
      241 gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat agctcctcgt tgaggagcat
      301 tacataacta tattgacaac tataaaaatt attcgataaa atagtgatgt tatcgcatat
      361 cttcactatc acccat
//
LOCUS       DAZKNO010000370          374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000370 DAZKNO010000000
VERSION     DAZKNO010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.397"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..309
                     /locus_tag="ACG8MY_002837"
     CDS             10..309
                     /locus_tag="ACG8MY_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0431931.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          144 a           51 c           74 g          105 t
ORIGIN      
        1 gaaatcgtga tgtctaaaag tgaagaaata gaaaacatgg tactaaaatt atatgatctc
       61 gttttaaatc cagagatcaa agaaaaggaa cgtatacttt tgatagacgc aaagacaggc
      121 ttagaaaaag gtcagtatta tcctaaagtc ataaataatt tggaacgtag tttacgtccg
      181 ttagcaatca ggggagaatt gtctaaacca gtatcaccgt tttatatgga gatatcaaca
      241 ataggaaaat ttgaaaaaga attaggtcga ggaatggctt cggcaccgat tacttttggg
      301 catttataaa aacgagttat tcatctcaat gaattaaaaa gtatagtcaa tctcataatg
      361 caggctaggt tcat
//
LOCUS       DAZKNO010000371          359 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000371 DAZKNO010000000
VERSION     DAZKNO010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.400"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>359
                     /locus_tag="ACG8MY_002838"
     CDS             4..>359
                     /locus_tag="ACG8MY_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0431932.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKI"
BASE COUNT          123 a           54 c           61 g          121 t
ORIGIN      
        1 aatatgtttg aaattttcaa aaagaaaaaa caagaaactt tttatgcacc ttgttccgga
       61 aaaatcattt ccatcacaga agtaaatgat cctgtctttt cacaaaaatt actaggtgat
      121 ggttttgccg tcatacctga tggttcaaaa atttatgctc cattggctgg tcgagtaaca
      181 aatatatttc ctactaaaca tgctattaat ataaaaagca actcaggcat tgattatttg
      241 attcatattg gaattgatac agttgaatta caaggagagc ctttttcgat ttgtgttcaa
      301 gaaaatgatg aggttactcc tgaaacctcg ttggttgatg tggattttga caaaataat
//
LOCUS       DAZKNO010000372          355 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000372 DAZKNO010000000
VERSION     DAZKNO010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.403"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..251
                     /locus_tag="ACG8MY_002839"
     CDS             57..251
                     /locus_tag="ACG8MY_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431933.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            241..>355
                     /gene="tnpB"
                     /locus_tag="ACG8MY_002840"
     CDS             241..>355
                     /gene="tnpB"
                     /locus_tag="ACG8MY_002840"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0431934.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLE"
BASE COUNT          124 a           59 c           69 g          103 t
ORIGIN      
        1 actaacaaat gcacctcaag atatacttga gctatcaaga agtctggagg ttttctatgt
       61 ataaattaaa tgagattgtt cctgtccaat taggaaacga aaagagtgca ccagaaaaag
      121 gtaaacccgc ggctgccaag ttagcgatgc gcatcaaaag agagaatggt tctgaagttt
      181 tgatctataa cggtattaac aattatattc ttcaagctat tttgaaagaa ctgttgtcca
      241 atgctttatg atttacttca aaccaaacat atttatattg tgtgcggaaa aacagatttg
      301 cgaaaaggaa ttgatggtct tgccagtctt attcaacaag agtatcaact cgagc
//
LOCUS       DAZKNO010000373          348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000373 DAZKNO010000000
VERSION     DAZKNO010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.404"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>348)
                     /locus_tag="ACG8MY_002841"
     CDS             complement(<1..>348)
                     /locus_tag="ACG8MY_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFQ0431935.1"
                     /translation="YSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFF
                     HILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDLDS
                     HKLISYPIIEKQQI"
BASE COUNT          115 a           69 c           44 g          120 t
ORIGIN      
        1 aatttgttgt ttttctataa ttggataact aattaactta tgactatcta agtcttcttt
       61 attcatatca attgcagtaa tttgactgtc ttcatatgtt cgataataat agattccttt
      121 gtcaacattg caacaagaag aataaattgt atattcatat tttccatcac caacatcaca
      181 caaacctttt tgttgttcta ctgaacctaa gatatggaaa aattgactaa tactttctga
      241 ctctgaatct ccagatacag aattcagctt cgtaaaagtc gctttaacaa aacgagatac
      301 tgaggataaa tctccaggca agcctatccc tcccataccg cggctata
//
LOCUS       DAZKNO010000374          345 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000374 DAZKNO010000000
VERSION     DAZKNO010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.405"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>345
                     /locus_tag="ACG8MY_002842"
     rRNA            <1..>345
                     /locus_tag="ACG8MY_002842"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          105 a           67 c          101 g           72 t
ORIGIN      
        1 ggtaaaacgc actggaggac cgaacccacg tacgttgaaa agtgcgggga tgaggtgtgg
       61 gtagcggaga aattccaaac gaacttggag atagctggtt ctctccgaaa tagctttagg
      121 gctagcctcg gaattgagaa tgatggaggt agagcactgt ttggactagg ggcccatctc
      181 gggttaccga attcagataa actccgaatg ccattcattc atatccggga gtcagactgt
      241 gagtgataag atccatagtc gaaagggaaa cagcccagac caccagctaa ggtcccaaaa
      301 tatatgttaa gtggaaaagg atgtggggtt gcacagacaa ctagg
//
LOCUS       DAZKNO010000375          327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000375 DAZKNO010000000
VERSION     DAZKNO010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.408"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>327
                     /locus_tag="ACG8MY_002843"
     CDS             <1..>327
                     /locus_tag="ACG8MY_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFQ0431936.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKG"
BASE COUNT          122 a           51 c           74 g           80 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaagg
//
LOCUS       DAZKNO010000376          323 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000376 DAZKNO010000000
VERSION     DAZKNO010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.412"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..318)
                     /locus_tag="ACG8MY_002844"
     CDS             complement(<1..318)
                     /locus_tag="ACG8MY_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HFQ0431937.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWIL"
BASE COUNT          114 a           61 c           40 g          108 t
ORIGIN      
        1 taaaatccaa ggaataaatt caaaaggaga tacattgtct ttcccttctt gtatttcttt
       61 ataatcagca tacccagaaa aatttagtcc agccatactc aatccttttt catttgtcgc
      121 atcgtaataa agagggtagt cagctatacc agcggcaata ccaatcattg cataatgagt
      181 atccaaatca tttacctttc gaaaattcaa cttataattt cttggagtaa tagtgactac
      241 ttcattgtaa gatatttcat aatcaaaatt ccttccaaaa taatgatcac ttgttacata
      301 agtaatagac gtacacatag ttt
//
LOCUS       DAZKNO010000377          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000377 DAZKNO010000000
VERSION     DAZKNO010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.413"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACG8MY_002845"
     CDS             complement(<1..>321)
                     /locus_tag="ACG8MY_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0431938.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAZKNO010000378          317 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000378 DAZKNO010000000
VERSION     DAZKNO010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.414"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>317
                     /locus_tag="ACG8MY_002846"
     CDS             <1..>317
                     /locus_tag="ACG8MY_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431939.1"
                     /translation="KNYYKYGQESYRKIVEELFPNLRNDLALYQVGPVQYSIKVKLPD
                     RASNDNFSQGSIGTSFCLVDSVKNCNPKMSVVENDTRLIDLDDYHNQLRKEAKFYNRT
                     LSI"
BASE COUNT          119 a           41 c           58 g           99 t
ORIGIN      
        1 taagaattat tataagtatg gccaagaatc ttataggaaa attgttgaag aattatttcc
       61 aaatcttagg aatgatttgg ctttatacca agtaggtcca gttcagtaca gtataaaagt
      121 taaacttcct gatcgggcct caaacgataa ctttagtcaa ggtagtatcg gtactagttt
      181 ttgtttagtt gacagtgtaa aaaactgtaa tccgaaaatg agtgttgtcg aaaatgatac
      241 aagacttata gacttggatg actaccataa tcagttaaga aaagaagcaa aattttataa
      301 cagaacatta tctatag
//
LOCUS       DAZKNO010000379          314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000379 DAZKNO010000000
VERSION     DAZKNO010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.415"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..231)
                     /locus_tag="ACG8MY_002847"
     tRNA            complement(147..231)
                     /locus_tag="ACG8MY_002847"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(195..197),aa:Leu,seq:caa)
BASE COUNT          100 a           64 c           62 g           88 t
ORIGIN      
        1 atagtttaac acataataaa agcgttatca atatcttttt tactcgtttt atgataatac
       61 ttgtatcgaa ttgatttgga ggtaattaca taaaaacaaa aaaaatatag caactctcta
      121 taagaaagtt gctatattga taattatacc ggcggccggg gtcgaaccgg cacgtcctca
      181 acggacactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caaatttaaa
      241 aataataact ggggtagctg gattcgaacc aacgcatgag ggagtcaaag tcccttgcct
      301 taccgcttgg ctat
//
LOCUS       DAZKNO010000380          309 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.416, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000380 DAZKNO010000000
VERSION     DAZKNO010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.416"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>309
                     /locus_tag="ACG8MY_002848"
     CDS             <1..>309
                     /locus_tag="ACG8MY_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFQ0431940.1"
                     /translation="RKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSC
                     GEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCP
                     "
BASE COUNT          102 a           51 c           60 g           96 t
ORIGIN      
        1 tcgtaaattc ttaagagaca acccattaga agcgatcgta acagacttga cttatgtacg
       61 agtcgggaaa aaatggaatt atgtctgttt cattttggat ctgttcaatc gagaaattct
      121 cggctattct tgtggagaac ataaagatgc cgttctagta aaaaaagcat ttagccgtat
      181 caaacaacct ctgacagagg ttgagatttt tcatactgat cgtggaaaag agtttgataa
      241 ccaagctatt gatgaattat taacaacttt tgacatcaat cgatcattga gtcataaagg
      301 ctgtccttt
//
LOCUS       DAZKNO010000381          301 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000381 DAZKNO010000000
VERSION     DAZKNO010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.418"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(156..>301)
                     /locus_tag="ACG8MY_002849"
     CDS             complement(156..>301)
                     /locus_tag="ACG8MY_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0431941.1"
                     /translation="NQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYIN
                     FDK"
BASE COUNT          111 a           38 c           47 g          105 t
ORIGIN      
        1 tttataaaag gacttataaa gattgattat ataatatatg aaaagctatt ataaaagaca
       61 ttagtattaa atagtttaaa aaaatgaaaa ataataaagg aagtgagtca attccagact
      121 cctgtgtaaa atgctataca atgtttttac catttctact tatcaaaatt gatgtatttt
      181 cttgaagaat aaatccattc atcatgtagg tccataagaa cggctccaat taagcgattg
      241 gctgatgttt gattggggaa gatgcgaata atcttttctc ttctgcgtac ttcttgattc
      301 a
//
LOCUS       DAZKNO010000382          294 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000382 DAZKNO010000000
VERSION     DAZKNO010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.420"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          112 a           48 c           49 g           85 t
ORIGIN      
        1 tttaaaaaag tgtgactttg tataaataaa ttagaagaag caaacgagga tgttaaaaga
       61 ataccatata gttaccaaac caagaaagtt ctcgtcaagt tcgctggtac gaatcaaatc
      121 aagtgaaaaa agggactgtg aaaaaaattg tgtcatagtt agtcactttt tccgaacaaa
      181 tagtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta
      241 tttttccatt tgtttctcta atactcagga caaaacactg attttacaac ctct
//
LOCUS       DAZKNO010000383          292 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000383 DAZKNO010000000
VERSION     DAZKNO010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.421"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>292
                     /locus_tag="ACG8MY_002850"
     CDS             <1..>292
                     /locus_tag="ACG8MY_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431942.1"
                     /translation="RTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNIS
                     TSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA"
BASE COUNT           84 a           61 c           58 g           89 t
ORIGIN      
        1 aaagaacatt cgtagcggat acctctgttg ctgaaaaaca ctgtttcatt agtcaaaaag
       61 ttcgttggtc tgtcattgct cgcttaaaag agaatacttc catgacggaa atcgctcgtc
      121 agaaaaatat ttccacttct tcggtctatc gtgtcatgaa gcgattctac cgcccattga
      181 atcctttcaa acagactttg ccaaaggtct tatgctttga tgaattcaaa tcggttcgaa
      241 gtgttgccag tgcaatgagt tttatcatga tggacggaca aagccatgca tt
//
LOCUS       DAZKNO010000384          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000384 DAZKNO010000000
VERSION     DAZKNO010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.422"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..>291)
                     /locus_tag="ACG8MY_002851"
     CDS             complement(119..>291)
                     /locus_tag="ACG8MY_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0431943.1"
                     /translation="NKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQ
                     QVTDTLVSMFTE"
BASE COUNT           87 a           39 c           50 g          115 t
ORIGIN      
        1 cgcactcttt atttagtttt tatgcaaaaa atcaagttat aagataaagt aggtgggatt
       61 ttgttaggga gcgtcaataa ttttgtgtaa ataaattgtc ctcctgcaaa ataattagtt
      121 actcagtaaa cattgaaact aatgtatcgg ttacctgttg aaaaccttta tggcttctgt
      181 ttagaaattt ttgattgtat gtatcaaaaa tgctgactag aaagcgttct agtgattctt
      241 cattttgaaa ctgctctttt ctacggctgt atcttttaat ttgcttattg a
//
LOCUS       DAZKNO010000385          264 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000385 DAZKNO010000000
VERSION     DAZKNO010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.425"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           90 a           42 c           53 g           79 t
ORIGIN      
        1 tggacgataa atttggacaa ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa
       61 ggctggtaat cttcgtcagc gttcatacac ggcattaaga tttggccctt aatgcgttaa
      121 cctttcagtg ttacggtatt acccgcaacg gatttagtat aacagatatt caaaagtgat
      181 accacttttt ttggctgtgt taaggttaaa aatacgtgat attacgatga atctatgcct
      241 aagcataatg ttataaaaat gtcc
//
LOCUS       DAZKNO010000386          262 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000386 DAZKNO010000000
VERSION     DAZKNO010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.426"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           90 a           49 c           57 g           66 t
ORIGIN      
        1 taaggggcag cgagtgtcga ccccttctag ggggattaaa aatacacccg tcctcacggg
       61 tgtatgggtt actgatagac ttgattttaa ctggtgtcag aaacttatgt ccagaaaggg
      121 tgatgaaatt ttggcaattt acggctattc aagaattagc acaagcaacc aagattacaa
      181 aacacaaatt caaacattag aaaatgctgg cgcagaaata atcttcctag aaaatcactg
      241 gattatcgaa cacctttgga ag
//
LOCUS       DAZKNO010000387          258 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000387 DAZKNO010000000
VERSION     DAZKNO010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.428"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>258
                     /locus_tag="ACG8MY_002852"
     CDS             <1..>258
                     /locus_tag="ACG8MY_002852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431944.1"
                     /translation="IMWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQA
                     NLLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESS"
BASE COUNT          119 a           38 c           40 g           61 t
ORIGIN      
        1 aaataatgtg gttagaaaaa ataaatgaca aaaaatttaa gtacaccgaa cgttatacgg
       61 atcccttaac tgaaaaaaag agaaaagtgt ccgttacttt atctagcaat tcacgccaag
      121 cttggaatca agctaactta cttttgaatg aaaaaatcgc agaaaaaata aaacgaaatg
      181 aagaattacc actaactttt ggagaattaa aaactaaatg ggatgaaaaa tacaagccaa
      241 ctgtaaaaga aagttcct
//
LOCUS       DAZKNO010000388          252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000388 DAZKNO010000000
VERSION     DAZKNO010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.429"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..94)
                     /locus_tag="ACG8MY_002853"
                     /pseudo
     CDS             complement(47..94)
                     /locus_tag="ACG8MY_002853"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           95 a           52 c           33 g           72 t
ORIGIN      
        1 gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa aagcccaaaa
       61 gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc ataaccatag
      121 gcatacaaag catacagata ttctccggat actttagaat cacatgataa aggtattctt
      181 aaactgggtg caaaaaacta agccctccta aaaaaggaca tccaattatt tgttcccact
      241 atcaaattga ca
//
LOCUS       DAZKNO010000389          251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000389 DAZKNO010000000
VERSION     DAZKNO010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.430"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..>251)
                     /locus_tag="ACG8MY_002854"
     CDS             complement(43..>251)
                     /locus_tag="ACG8MY_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0431945.1"
                     /translation="LYAYPQGTVASWITRQRRIKSLPASFVYDLSLASSMNMTDVYEK
                     LLILENEYDQFTSNQQRKVKKQID"
BASE COUNT           70 a           41 c           39 g          101 t
ORIGIN      
        1 tataacttat accttagata ttgcttttcg aaaatagcta ttctagtcta tctgtttctt
       61 tacttttcgc tgctgatttg aagtgaattg atcatattca ttttctagta ttaataactt
      121 ttcgtataca tcggtcatat tcatactaga agcaagactt agatcatata caaatgaagc
      181 aggtaatgat tttattctac gttgtcgggt tatccagctt gcaactgttc cttgtgggta
      241 tgcatataac t
//
LOCUS       DAZKNO010000390          247 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000390 DAZKNO010000000
VERSION     DAZKNO010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.431"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           92 a           50 c           33 g           72 t
ORIGIN      
        1 aactgttaaa gcaataattt cattaccgtt gttacatcac cgttaccatc aaataattta
       61 ccatcatttt ctaatgacgc cgtttgtata attaagcgag acaaataaaa aagccattta
      121 tgttacactc aaatcgtaat acccgctaaa gcatacaagg agagtgaaca taaatgactt
      181 atacccatct tacaccaaac gagcttgtaa tgatagaagt atattttcat caagaaactc
      241 cggttgc
//
LOCUS       DAZKNO010000391          240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000391 DAZKNO010000000
VERSION     DAZKNO010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.433"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(72..158)
                     /locus_tag="ACG8MY_002855"
                     /pseudo
     CDS             complement(72..158)
                     /locus_tag="ACG8MY_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT           89 a           45 c           26 g           80 t
ORIGIN      
        1 aatgaaatac ctctttgtta ctcataatta taccataatt agtcacgaat gcaaccaata
       61 gaaaaaaata cttagaacgg tagatcatcg ctaataacaa tgcattgtcc accaaatgga
      121 ttttgtgaag cattaaatac attgttatta attgtcgctg cttttaacta ggcctctccc
      181 cttctattat aaaagaagat atatattatt tcttaattac ctactacctt aaaccaaaat
//
LOCUS       DAZKNO010000392          236 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000392 DAZKNO010000000
VERSION     DAZKNO010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.434"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>236
                     /locus_tag="ACG8MY_002856"
     CDS             <1..>236
                     /locus_tag="ACG8MY_002856"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0431946.1"
                     /translation="NETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYG
                     AGSYANANSPVQIELQHTHDKATFEKNYKAYVELA"
BASE COUNT           74 a           45 c           58 g           59 t
ORIGIN      
        1 aatgaaacag caacaggacg caatgaagcg cagtatatgc aacgttcatg gactagcgct
       61 tatactgctt atattgtggg agacggcgga attgtttatc aagtcggtca acctggttat
      121 gtacagtacg gtgctggttc gtatgctaat gccaacagtc ctgtgcagat tgagttacaa
      181 cacacacatg ataaagcaac gtttgagaaa aactacaagg catacgttga attggc
//
LOCUS       DAZKNO010000393          234 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000393 DAZKNO010000000
VERSION     DAZKNO010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.436"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..>234)
                     /locus_tag="ACG8MY_002857"
     CDS             complement(46..>234)
                     /locus_tag="ACG8MY_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0431947.1"
                     /translation="HQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRL
                     AKRILDNERGSDTSGEAA"
BASE COUNT           59 a           59 c           48 g           68 t
ORIGIN      
        1 gcatcctcgg atctgatttt ctccggcaga agcacaatca ttcaattacg ctgcctctcc
       61 agaggtatca gatccgcgct cattatcaag gattcgcttc gccaatctct gttgtcgaat
      121 ttggatcggt gtttcatacg ccaacgtgcc atgtaagcgt agatagttcc accaatgcac
      181 ataatcaaac aattctagac gtagctgtgc cagtgtctca aattggtatt gatg
//
LOCUS       DAZKNO010000394          232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000394 DAZKNO010000000
VERSION     DAZKNO010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.denovo.438"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           97 a           33 c           42 g           60 t
ORIGIN      
        1 tttcacacgt taggtaaatg aacttgtggg cggaggaaag taagaagcca actccttaca
       61 aaaagggagt tggctttaag aatttatttg tttactttcg cgatcggaca aaatataaaa
      121 cgatcgtaga aacaagacta actaaacccg ttaaataaaa ttctggccga gtgatcgtaa
      181 taaaagaaaa actagttcca aaaaagtatt tataaataat taacagaaat aa
//
LOCUS       DAZKNO010000395        15522 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.055, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000395 DAZKNO010000000
VERSION     DAZKNO010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.055"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            470..1333
                     /locus_tag="ACG8MY_002858"
     CDS             470..1333
                     /locus_tag="ACG8MY_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0431948.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            1433..1915
                     /locus_tag="ACG8MY_002859"
     CDS             1433..1915
                     /locus_tag="ACG8MY_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0431949.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(2178..2645)
                     /locus_tag="ACG8MY_002860"
     CDS             complement(2178..2645)
                     /locus_tag="ACG8MY_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0431950.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            2768..3619
                     /locus_tag="ACG8MY_002861"
     CDS             2768..3619
                     /locus_tag="ACG8MY_002861"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0431951.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(3870..5828)
                     /gene="nagE"
                     /locus_tag="ACG8MY_002862"
     CDS             complement(3870..5828)
                     /gene="nagE"
                     /locus_tag="ACG8MY_002862"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0431952.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(5825..6697)
                     /locus_tag="ACG8MY_002863"
     CDS             complement(5825..6697)
                     /locus_tag="ACG8MY_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0431953.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(7039..7911)
                     /locus_tag="ACG8MY_002864"
     CDS             complement(7039..7911)
                     /locus_tag="ACG8MY_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0431954.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            8039..8608
                     /locus_tag="ACG8MY_002865"
     CDS             8039..8608
                     /locus_tag="ACG8MY_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0431955.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8675..9412
                     /locus_tag="ACG8MY_002866"
     CDS             8675..9412
                     /locus_tag="ACG8MY_002866"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0431956.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            9643..10911
                     /locus_tag="ACG8MY_002867"
     CDS             9643..10911
                     /locus_tag="ACG8MY_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0431957.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            11398..11862
                     /locus_tag="ACG8MY_002868"
     CDS             11398..11862
                     /locus_tag="ACG8MY_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0431958.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            11846..12475
                     /locus_tag="ACG8MY_002869"
     CDS             11846..12475
                     /locus_tag="ACG8MY_002869"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0431959.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            12829..14307
                     /gene="msr(C)"
                     /locus_tag="ACG8MY_002870"
     CDS             12829..14307
                     /gene="msr(C)"
                     /locus_tag="ACG8MY_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0431960.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            14354..14800
                     /locus_tag="ACG8MY_002871"
     CDS             14354..14800
                     /locus_tag="ACG8MY_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0431961.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(14999..15514)
                     /locus_tag="ACG8MY_002872"
     CDS             complement(14999..15514)
                     /locus_tag="ACG8MY_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0431962.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT         4990 a         2768 c         3182 g         4582 t
ORIGIN      
        1 ttcttagaga atgtgagaaa aagtaaaaga atagcgtgtt ttaaagatgg aacttttttt
       61 gttttttatt tatctgacaa aaatgaagtg attttgttag aaataaataa ttgttccaat
      121 tgataaaaga ggtatgatgt atcgcaaaga aaaatccaag cagcgttctg atcagcagtt
      181 tgaacaacaa tttgctttgt acagtaggta gtaagcggat taactaaggg aataaaagat
      241 atttttaccg ctttgtttac ttagaatctg tcttccccaa aatttatttt tctaaaggta
      301 aaacatagat aggcagataa taccaattat ccgttacgaa acgctgcttg agaattttct
      361 ttagatccat acaacatttt ctacatctga ctaaattggt tcctgattca tcaagtaatt
      421 gactttcttt gataatatga taaaaaggca atcaaggagg aagataaata tgcttaaaga
      481 tctgaaccaa ttgatcgatt acatcgaaga acatttgaca gaaggacttt cctataaaat
      541 aatagccgaa aatacaggaa tctctgaata tcaattaaaa cgcatcttta cgtttattgc
      601 agggatgtcg ctaatggact atatcaaaaa ccgtagattg gctttggcta atcgcgatct
      661 tctagatgga tcaagagtga cagaaacggc gtttagatat ggatatgaaa cagtcgaagg
      721 tttttcaaga gcttttcgtg attggtcagg atatctgcct tcagaaatca gtagatacgg
      781 catacaaaaa tcttttccac gcttgacatt ttttattgat gtcagaggag gaaaatcgat
      841 ggaatttcgt atcgaaaaga aagaacagtt ttatgtggca ggtgtggcga aaaaagttcc
      901 tgtcaaattt gaaggagtca atcaagaaat acaaaagtta gcgcagacga tcacggagac
      961 tcaaagggaa gagatgcata agttaggaga tttatatcca aatcaagtaa tcaacgtttc
     1021 ttatggattt gatggagacc gcttggaaga aaagggcagt ttgatgcaca tgattggctt
     1081 tgctacgaca aaagaaaatc cgtacgctga tttagaaatg cttgctatag aagaacacac
     1141 ttgggccatt tttcctaata aagggccatt tccccaaact ttacaagata cttgggggaa
     1201 aatctatgca gaatggcttc cttcttcaga ttatgggtta gtcgaagctc ccgagatctc
     1261 ttttacaaaa tggggagagg atttcagtaa tgtttacagt gagatttgga ttgcagtgaa
     1321 agcaaaaaaa tagttaaaaa ataaaaatat ccctagaaaa aagagcgtaa tcgtgtcatt
     1381 tccctttaat tttatgtaag atgaacttaa tataatagaa agggtgggag aaatggcaaa
     1441 gtataggatc gaagaaaaac cagattttac ggtgacgggt attggtgtgg aattgaagag
     1501 tagcaatacg gattttgtcg gtttggctgc agagaaacaa gcatttgagc aaaaagttat
     1561 cggtaatgga acaatagatg cattgaaaca agctgctgaa aatcattatt tctttacagt
     1621 caacgaaccc tataacggga aaatgatgta ctatgtcggt gttcaaacga gtcaggatgt
     1681 tccaaacact acgcgaatca tcccattccc aaaaggaacg tatcttgttg taagcgcttt
     1741 agcagaatcg cctgaagaat taagaaatga tttgacgaat acggcctttg ggcatgtttt
     1801 aagagaagta gaagattacg cctatgttgg gggtccaaat gctgtagtcg aaatgggagt
     1861 taccaacggg aaatatatgg gagaaatctg gataccggta gtcaataaac catgataata
     1921 tagaggtcgt gaaagtagcg ctttgacctg agcattagag aggaaaaatg ggaaatagct
     1981 ttccatattt tgcatttttc agaactaatg ccgagggtca gctacttgaa caccgtttat
     2041 caagacgtca tgacttcagc gctttgacct gagcattaga gaggaaaaag gggcatgaca
     2101 aggcttttgt cccccggaga ataaaagaat ttatgtagac gattccgatc agaaaaatgc
     2161 aggcacagta gagagaatta taaaaatctc tctacgaggc ctgtgttttc tggtcttttt
     2221 tctttttcca aaacggctat tttatgtatc agactatcaa gtgtgatctt agccatttct
     2281 tcttctgcct ttttctgcag ccgagcgtag ctttcttcta aagcttcttg gatattcgca
     2341 ccaacgacgc aatttggatt tgtttttgga tcgacttgga taagctgtgt attaccttcg
     2401 atgctgcgat agacatctag taaagaaatc tgatctggac ttttggcaag ggaaggctgc
     2461 ggttttccgg tttttgtaaa gatcaaaccg gattttttga gctgactcat gattttacgt
     2521 acatttgctg catttgtttc gatactactg gcaatcatct cactagaaag tgatcgggta
     2581 tcttgaaaca tttcaatata ggcaagtaca tggattgcat cactaaattg cagagaatac
     2641 ttcatcagtt gtcatccttt catttttttt gcttgactta tcttaccaca ctctttatga
     2701 taaaggtgta atttataaat gtacagctgt aaatcttaac aaataaaaag aatagggagt
     2761 ggcaaatatg cattatttag taacaggagc aactggaggt tttggtggtt atgcgctgga
     2821 attcttaaaa aagatggttc cacaaaatga aatctatgta ctagtacgta gtgaagaaaa
     2881 aggagaagca ctgaaagaag ctggatttaa tatacgtatt ggtgattatg atgatttaga
     2941 ctcaatgaaa gaagcgttga aaggaataga tcgattgctt tttgtctctg gcgttccagg
     3001 aaatcgtcaa gccgaacata agaatgtagt gaaggcagca caagcagctg gggtatctta
     3061 tatcgcttat acaagttttg cagatgccga tcactcgatc agtgtattag cgccagatca
     3121 ccaattcact gaaaaaatga ttaaggattc aggtatttcc catacatttt tacggaataa
     3181 ttggtatgta gagaatgaat tacctatcat tgggcaggct ttaaaaactg gtcaattcgt
     3241 ttatgctgca ggaaatggaa aaacaggttg ggcattgaaa cgtgaatatg cagaagctgc
     3301 agcaaaagta ctagtagaaa gtgactctcc agaaatctta gaattatccg gcaatctaac
     3361 tgactatgaa gaattagcca aggcattaga gcgtgcgaca ggtaaagagt tagaaattat
     3421 tgaagcaagt gatgcagcgt ttgtcgaaaa tctaaaagaa gccggattcc ctcaagaagc
     3481 agctgacatg tttttagcga tccaacatga tattaaaaag gatcagttag ctatttcctc
     3541 tgatgattta gaaaaagtat tgggaaaacc attaacttct cttgaagcag cattaaaaga
     3601 aatattggat caggaataat gtataaagag gttgtgaaaa agccgattag ttttgagtat
     3661 gaagaaaaat tttgtgaaaa agctgttctg catcaagtaa taagaagagt caaataaaaa
     3721 atagctcttc atatgtttcg tttagctgga cttattaccg cggagttggc aggagaacgc
     3781 cgtttttatt cggaaaaagg agctgagaca agatttttgt ctcagctcct gtttgtttat
     3841 cgataaaaaa gtctggggaa gacgatggct caggctttgt tggaaacaga gccgatttta
     3901 tcatttgctt ttacatcacg tgcgggttcg atttctaagc tttggacttt gtcgccattt
     3961 gtgaatacaa cgaccatatc cgtgtttttg cctgcagatt gaatagcggc aagatcaact
     4021 aaagcaatca attgacctct tgcgactttt tgaccttctt ctacatataa agtgaatggt
     4081 tcgcctttta agtcaacagt gttgatgccc atatgaagca agacttcgat accagcatct
     4141 gtttggattc cgacagcatg tttcgttggg aaaatgtttg tgatcgtacc agcgattgga
     4201 gagaagattt ctccattttc tggtaataca gcatagccgt cacccatcat tttttccgcg
     4261 aatacatcat ctgaaacttc agtgattgga atcagtttcc catttgcgat agcgtaaata
     4321 tcttgttcga tcgtttcacc ggcaaatgtt tctgttttaa ccacggtgtt tgtttgtgca
     4381 ggtcgtgctg cagcaccacc atgtagtttt gccatctcat ctgcaacgaa ttgtacttct
     4441 gtacctacga tgacttgcgc attttttccg tcgatgatct tcataccagg tacgccagtg
     4501 gctttgattt tattttgatc gatcaggcct gtatctttga cttgcaaacg aaggcgtgta
     4561 gtacagttat cgatcgaagt tacattgtcg gctccgccta atgcagcgta gattttgctt
     4621 gcttgtgccg catatttatt gtctgaatta gtcgttgcag tatcgtcatc tgaaagatct
     4681 gcttcctcgc ggccaggagt catcagatgg aattttttga tagcaaagtt aaagccaaag
     4741 tagtaaatca ctgctgttac taatccttgg acgatcaaca tcaatggctg attcgcaatc
     4801 ggatttttca aagataggaa gtaatcgaca aatccggcac taaaggcaaa tcctgctgtc
     4861 caatggaaga atgcactcac tgctaaagaa atcccagtga aaattgcatg caagacatat
     4921 aatggccaag caacgaacat aaatgaaaat tcaagtggtt ctgtcacacc agtaaagaat
     4981 gaagcaaaag ctgctgctag catcaaggaa gctgtttttg ctttgttttc tggacgtgca
     5041 tttctgtaaa tagcatatgc tcctgctgga agcccgaaca tcatgattgg gaaaaatcca
     5101 gcttgataca ttcctgttac gccttttgtt ccttcagaag ccaaaaattt accaatatca
     5161 ttgattcctg ctacgtcaaa ccagaacacg gagttcattg cttgatgcaa tcctgtaggg
     5221 atcagtaaac ggttgaagaa gccataaaga cctgcaccac cccagcctaa actcaaaatg
     5281 aatttagcaa aagaaactaa agcagagtaa accggaggcc atacgagcag tagcactgca
     5341 ctgatcagca acatgacaaa ggcagccaag attggcacta gacgcttgcc gctaaagaaa
     5401 gataaagcca tcggtaattt tgcttcgtgg aaacgattat aaagagctgc cgcaatcaat
     5461 ccggcaataa taccaataaa tacgttactg atctgagcaa aagatggatc aactgattct
     5521 ggtttgatat tcaataaagt agctactgaa tcagttttta aaacatttac tggaacttca
     5581 aatgcgacca aacctgccaa ggcagcagca cctgatttgt ccactgacat ccctagtgct
     5641 aacccaacgg caaacagcaa aggtaattga cctaaaactg cgttaccgcc agcttgtaaa
     5701 aaatgagcga caatgtcgtt actataagat gcccaccagt ttccgatccc tactagtaaa
     5761 gcagcagcag gtaagaccgc aactggaagt tgaagggaac gtcccattcg ttgtagatat
     5821 gctttcaatt tttttcctcc tcatgattgt taacatttgt ttgtacgatt gcgcttctta
     5881 aacgctcgat atggatggcc aagtaaccca gttcttcttt cgtgatcgcg agctggtatt
     5941 ctttggtcag caacacacgg atttttttag aaatcttata gctttctggg tattttcgtt
     6001 tgaccatttc tacgatttcc gtgtctaaaa cagcatattg ttgcgcatgg gttcgttgta
     6061 gaagcagtcg taaatgattc accagtcttg aataattcag tgaaaatggt tctgaatgga
     6121 tatctgtttc caattctgat tccactaaat ggatgatgtc agatacaatc gtcacttcac
     6181 ggatactgcg atggttattg ttccgaccgc ttcgtccgct gtgaatatgg attgcgatat
     6241 atcctgcttc gtcataagta tatggaatcc caagctcttt tgtcagatac tctactgccc
     6301 attgagcaat actgaattca tcactgtaaa gcacttcgat ttctcttaaa agtttgttgc
     6361 ggatgacgat accgttatta atgttctccg cagaaaaagc aatgtggtcg gtcaaagcga
     6421 tcaatacatg ttcattcaac ttttccatca aaacagtttc cgcatgatca atgatcgttt
     6481 ctgctgcaaa taaaaagcgt tcatcaatct gattaagtaa attttgcaac ttgatttgac
     6541 cttctggttc catgacaaat atccgttcga catcacgatc aaaaatcaaa tcatttcttt
     6601 ttctgtcaaa tccaatccct tttccaatag ctactttttc ctgtccaccg tcatctacca
     6661 atactgcatt ttgattaaag atctttttga ttttcatgcg cgcacccttt ttgaagattt
     6721 ggttttcacg tttgctagac cagttgattg cggccaaaca aaaatagaca cgaaaaacct
     6781 cctagggaaa ccatgttcct cagagatttt tcatgcctag tatgatctag tcacatgaaa
     6841 cgaattattg ttaacgtaat caattttata tagacagtta tgttttgtca attctttttt
     6901 tataattata gtaatttact ggaattatca gaaataaaag gaagaaataa attaagaaac
     6961 ttatgttatt taatcgttgg atcttttgct tatcttattc gttgttaaaa agctgaaagc
     7021 gaaggaaaaa aactctactt aactgtattt ctccgttagg acatctaaaa atttacgagc
     7081 agcaggagaa agaggctggt cttttttcca aatgagattc agtgaagctt gtaatggcgg
     7141 atcgagtggg atgaatattt tttccgtccc ataagtattg atgatcccat ctagagctaa
     7201 tgcaccaaaa gtcccgttat cgaccatcaa agaagcattg tacaataagt tatatgttcc
     7261 gacgatcgat aactgatcta tgctgaaacc tagccattca gctagttgac tgtccactga
     7321 cgtttgccgg gaaatgatca aaggaacagt tttcagttcc tgcggatgga tgacggtttg
     7381 ctttgctaaa ggatgatttc gatgtatcaa aagtccccaa cgatcttggg ctggcagtcg
     7441 aaatgactgg tatttttgtt tgtttgtcgg ttcgattacg agtccaaaat caagtaatcc
     7501 gctatccagt ttgtctatga tatcatctgc attcccgcta tataaatgaa actggatatc
     7561 aggatgcgcc ttgatcaacg aatgaaggct atcagaaatg aaagagattg ctttagtttc
     7621 gcctccgcca atgtagattt ctccagagat gattgcgtca tttgttaaat tgttcgccgt
     7681 tttttctacc aacgacaaga tttcttttcc ttttgaataa agatatcgac cagaatccgt
     7741 caactgtatc tctcgggagc cccgagtgaa aagtgtcaca ccaagttctt cttctaaatc
     7801 tttcagttgt tttgagagag taggttgcgt gatatgcaga gattttgctg ctcggctgat
     7861 atttttttct ctagctactg ctaaaaaata cgtcagtata cgaatatcca ttttttccct
     7921 cctgttttta aaaaagcata ccactaagta tagttaaaag gaatagttaa gtatgtttta
     7981 taagtattag acatgataag ccgtccgtta taaagtaagg ataaaaggag ggagcaaaat
     8041 gacaagcctc atcgaaaaac aaatcgtcaa taaagagatt cgagtaacgg atccgctctt
     8101 tgaagtgatc catcagatcc aagctgaaaa cgaagagcct ttagcagcgc tgaatactgg
     8161 gtaccatgaa ccagcagata tccgcaaacg gctagaaaat atcattagtg acaaagtaga
     8221 tgatacggtg actgttctgc tgccttttta tacagatttc ggcaaacata ttagtatagg
     8281 gaaaaatgtc tttatcaatc gtcaagtgat gttcgttgac ttaggtggca tctgtttaga
     8341 agattcagta ttgattggtc ctcgtgtcaa cctcatcacg gtgaaccatc taaccgatcc
     8401 aaaagaacga agaggcttgt cagtaaagcc aatccacatc aagaaaaatg cttggatcgg
     8461 agcaggagca actattctgc caggcgtaac gattggcgag aatgccattg ttgcagcgaa
     8521 tgcaactgtt acgaaagatg tcccagacaa taccatcgtt gcaggaatcc ctgccaaaat
     8581 cgtcaaacct gtcgaacgga ttatttgaac agtaagatga aataagagat agtgagtata
     8641 agcaagagat ttttcaaaac aggaggagaa tagaatgaat gatttatcag aaaaagtgat
     8701 tgtcatcatg ggcgcttcaa gcggaatcgg tgaagcaaca acgaagctat tagcagaaaa
     8761 aggggctaaa ctagttatag ctgcacgccg agaagatcgc ctaaaagcaa taaaagaatc
     8821 tttaccagaa gctgagctat atattcaaac agcagatgta agagattttg cacaagtcca
     8881 agcagttatc gatttagcga tggaaaagtt cggtcgaatc gatgtattgt acaataatgc
     8941 aggaatcatg ccaaccgctc cattagttga aggtcaccgt gatgaatggc aaaatatgct
     9001 tgatatcaac atcatgggtg tgttaaatgg aatttccgcc gtacttccaa tcatggaaaa
     9061 acaaaaatct gggcatatca tttcaactga ttctgtggcg ggacatgttg tttatcctga
     9121 ttccgccgtt tattgcggaa caaaatttgc tgtccgtgca atcatggaag gattacgtca
     9181 agagcaacgg caaaataaca tcaaatctac gatcatctct ccaggagctg ttcaaactga
     9241 attatatcaa accatcagcg acaaaaaggc tgctcttgaa cttcatgaag cgcaaaaaga
     9301 atggggattg acctccgaag atatagcttc tgctgtagca tttgcgatag aaacgccaga
     9361 ccggatgtct gtcagtaaca tgattatccg tccgacgaca caggaagtat aatccataac
     9421 ggataatatc caaagagaat tacttgttaa atagttattc aaacgaccat caatttaagg
     9481 cgtgaatgga gaagaatatt cttcatttac gtctttttat gtagaaaaag caaatttatc
     9541 aaaaagaaac aaatgttatt taaatatgga cattgacttg cttttacttt tttagttaca
     9601 ctgttattta ggaaaagttt cataattcaa aggagaaaaa agatgaaagc aaagaattta
     9661 ttatttagcg ttattctttt aggaaatcta gcagttgcca acacaacagt gctagctgaa
     9721 gaatcgacag aaactgtcgc aactcagaca tcgagttctt cagaaacagg aattgaaaac
     9781 gatacagaga gtactttgca gtcagctact gtctcagaaa atacaatttc aaccgaagaa
     9841 ccaacaactg aaacaacaga acaaacacaa accgaagaca gtacatcagc agaagctcag
     9901 aacaatccag agaatgcaga agaaatcacg aaccaattga cagatgggca aacattgaca
     9961 catacccatc aagcagattt gtctgaattc gaaaactata ctggctatgc tttttatgct
    10021 cgctcatctg ctaattctca acaagcgttc attgatagta ttgcctcaac tgcacaaagt
    10081 ttggcaagtg ccaatgactt atatgcatca gtcatgattg ctcaagccat cgttgaaagt
    10141 ggctggggga acagtacttt ggcttctgcc cctaattaca atctatttgg gattaaagga
    10201 agctataatg gccaatctgt tacgatgcct acttcggaat atgtgaatgg gcaatggatt
    10261 acggtgaatg ccgcttttag aaaatatcct tcgtacaaag aatcattgca agataacgta
    10321 acggtattga agacgacttc ttttcagcca ggcgtttact actattccgg tgcatggaaa
    10381 agcaatacga actcttacaa agatgctacg gcttggctaa caggtcgtta tgccactgcg
    10441 cctaattatg gttctacatt gaacaatgta atcgaaacct ataatctgac acagtatgat
    10501 acagcaccaa cttcaacttc tccgatgtat cgtctgtata accgtcatac aggagaacat
    10561 ttatatacat tgaatgccgg agaaaaaaat tatctgccaa ctgtaggctg ggaatatgaa
    10621 gggattgctt ggcaagcacc aaacagcgga caacctgtct accgtttata caatccttac
    10681 agtggagacc atcattacac aatggcacaa tctgagatca actctttaac aaaaatcggt
    10741 tggcgttatg aaggtctaag tttttattct ggtggttcca agccaattta tcgactattt
    10801 aatccaaatg aaaaaactgg tacccatcac tataccttga gtgctaagga aagagattgg
    10861 ctaacaccga tgggctggag atatgaagga ataggatttt atggttatta aaaagaggta
    10921 gtgaaaaagc tgccctgcat caagtagtaa gaacagccaa acaaaaacag attttcatgt
    10981 tttttgtttg gttgaactta ttaccgcaga tgcaagtttt tgaacaccgt ttactcaaaa
    11041 aaggttgtga caacatcttg tcataacttt tttattactg tttaagcata ctgatccaat
    11101 ttctaatatt cgacgggatt cttgcaaaag gcgatttcgg aaatttttgg cggtgtaaaa
    11161 ttttctactg cagaagagct atcttctata gtttgataag aatcaataat cttggtttta
    11221 gcgttgtgct ttctttatca ttttttccgt tcattctaca aaaaatttct tttctctata
    11281 cttttctatt gagagcgttt tttttattac acagctgatt ctttgacttt tttctttttt
    11341 tattctatta ttgacaagtc aatcgttgac gagtcaatta ttagaaagga ggaaattatg
    11401 tctttagaag agattagtca ccttttatat caaatcaaat tagccgatca aaaaatcgtc
    11461 cagatctttg aagaaaatat cggtataagt cttactagat acgagatcat gatgagactg
    11521 aaagaagaaa atccattgct acaaagcagt ttgcaagaaa agttgcagat tgatcaagga
    11581 gcagttactc gtcatctgaa ggtattggaa gcaaatggct atgtttcgcg tcagagaaat
    11641 cctcagaaca accgggaagt gtatgtagcg ctaacagaaa aagcccagaa gaaaatcgag
    11701 cattgtgaaa tggaccataa agatacaggt tccgtgctat ccccggcttt cagcgatgta
    11761 gaaatgaaac aatttctaac acttttagat aaattgaata cccaactttg caatataaca
    11821 acagaaacga aagaggttaa taataatgga acaaaaaaat gatttttcaa caattatgac
    11881 tggtagaaga tccgttcgcg tatatgatga aacatacaaa atcccacacg aagaaatgct
    11941 ggaaatgatc aaagaagcga cgactgcacc ttcttctgtc aatatgcaac catggcgctt
    12001 tgtggtcgct gaaagcgagg aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac
    12061 gagacaaaat gatacttctt ctgcaatggt catgattttt ggcgatatgc aatgttacga
    12121 atatggcgaa gaaatctata accaagctta cgaatcaggg aagatgccaa aagaagtcag
    12181 agatcaacaa ttagctgcga tcattcctta ttacaagagt ttttcaagag aagaaatgaa
    12241 cgatgtagtc aaagtcgatt ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga
    12301 tcatggctat gaaacaaatc caattggtgg attcgaagca gatcagttgg cagaggcatt
    12361 tggtttggat aaagaccgtt atgttccagt tattatttta tcaataggaa aagcggtaga
    12421 agaaggttac gagtctgttc gtttagcacc agaaaagatc actacgttcg aataaaaggt
    12481 tttcagttac tattcagtag ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa
    12541 aaggaggtcg gaaaaatgac tgcatcgatg aaattacgtt tcgaactttt gaataacaac
    12601 taaataaatt caaaagacat gtataaacaa caatttcatg gatggagtat gtccattttt
    12661 tcgaacgaca gtagaatagg tgaaagctat gacagatttg gtgatttagt catagttatt
    12721 tcgttcagag taggaaaaga tgtgatgcag tttttttgca aacagatttt tctgctcttt
    12781 tttagtatcg ataattgttg ttttacatga aataggagtg taaaacatat ggaaaattta
    12841 gcagtaaata taacaaatct acaagtcagc tttggcaacc agctagaact atctattgat
    12901 tctcttcgtg tctatcagca agatcgtata gggatcatag gagaaaatgg tgtcggtaaa
    12961 tcgactttgc tcaaactaat agccggtgaa ctttttcccg atcatgggaa aatccaaaca
    13021 gagatcacct tcaactacct gcctcaatta acctatctcg ctgaagcaaa ggacctaaat
    13081 ttggaattag ccagtcattt ccagttaaga ctggaagaaa cttcagagcg gaaatggagc
    13141 ggaggggaag aacgaaagat cgagttgata cgtcttcttt cttcttatga acaagggatg
    13201 cttctagacg agccaacaac ccatctagat agaaaaagta ttgatcgact gattgaagag
    13261 cttcgttatt attatggtac gctggttttt gttagtcatg atcgctattt tctagatgag
    13321 ctagcatcga aaatctggga agtaaaagac ggagaaatcc gagagttttc ggggaattat
    13381 agtgcctatc tcactcaaaa ggaattggag aaaaagactc agctacgaga agcagagtcg
    13441 atcatgaaag agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa
    13501 aagttggaaa aagtgtccag taaaaagaaa aagcaacaaa tcagaccgga tcggttgtct
    13561 tcctctaaac aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa aacattagag
    13621 agaagactcc aaaaaatagg agaaacaacc aagccgcagc agatgaaaca aatccgtttt
    13681 ccagtaccaa aatctcttga actccacagc cgctatccca tcatgggaca aaatgtccaa
    13741 ttggaaagaa gtgggagaac attactggta aatggtgatt ttcagttttc tttaggtaaa
    13801 aaaatcgcga ttgtcggtga aaatggttca ggtaagacaa ccttattaga acatatccgc
    13861 aaacaaggag aaggaatcct tctctctccg aaagtaagct ttcaagtata tcagcaaaag
    13921 ggttatcaaa tgacatctga agaatccatc attcgttttg tcatgagaca aacagagttt
    13981 tcagaatcgc ttgtccgtag tttgctgaat cacttagggt ttgctcagga aactttgacg
    14041 aaaccgttat gtacattaag tgggggagaa gcgacccgtc tgacgattgc tttgcttttt
    14101 actaagccaa gtaatgtgtt gctgttagat gaaccgacta attttattga tatggcaacg
    14161 atcgaagctt tagagaagct aatgcaaata tatccgggaa cgattttgtt tacttctcat
    14221 gattcttact ttgtcgagcg tacggctgat gaagtttatg aaataaaagg gcagaaaata
    14281 aaaaaagtac ttacgagaaa tttttaaatg tgctacgatg attttcgtga caaatttaaa
    14341 ggagtggtac agattgccta caaataaaaa agaaggaata atttttacaa caatgatgtg
    14401 ttttttgatg gtcttgggga tgagcgcgta taatttatgg ctgcatcaag atcttcattt
    14461 atcaagtttg ttgattggtc tagtgcctgg attcgtggtg gctttcattt tggatgtctt
    14521 catcatcggt gtgctggcga aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa
    14581 ggtccaaatg atcttaacta tttcatgctt gatggtttta ggaatggtga catgtatgtc
    14641 tttattcggt gtgatcatgg aacaaggaat acctgatcaa ttattctctg cttatatgaa
    14701 tgcttggaaa atgaatgtga ttgccgcttt accgttgcag ttgatcatcg ttggcccgct
    14761 ttcaagaagt gtgctaaaag caattcaaag taatgaatga tggaacactg ttatttgaaa
    14821 aaaggccttg gcatgctttt tgtcatagtc tcttgaatgc agttctagaa aaatagtgat
    14881 ccgaacgata ggttttggcc tgtcgttcgg atcgcttttt cttttccaac tgtattgctc
    14941 ttttattgaa gataatttgc tcttcaatag atatttctag aaaggttctg tttatccctt
    15001 actcaaaaaa ctgctgcatt tgttctctta cttgctgaca ccattggagt tcctgctgac
    15061 aaagcataat tctgcgctgg atcacaacat aacgtccaaa ttcttgtttc ttgtctagcg
    15121 agaggtcatt caattttttt tgattttttt gcatgatttt gttcagctgt tgttctctag
    15181 tgaatagcag tccagtagct gttgttttat cgagcgtcga aaaagcatag atttttgcta
    15241 gaaattcatc tttttgcgta ggagcgggag tttcctcttt gatccattcc tctacaagag
    15301 cacgtccttc atcagtcaag tggtagatct ttttttgaga gtgctcgtct ttgtccaaaa
    15361 cggtcacata accctgctgg tctaacttgg caagcgttgt atagatctga ctgtgatggg
    15421 cttcccaaaa aattgtgatc agctgcttca attcataccc agaacgtggt ttggataaaa
    15481 gcacgcataa taatatatag gctaaagtat tcataaaaat cc
//
LOCUS       DAZKNO010000396        10041 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.099, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000396 DAZKNO010000000
VERSION     DAZKNO010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.099"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            353..523
                     /locus_tag="ACG8MY_002873"
     CDS             353..523
                     /locus_tag="ACG8MY_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0431963.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            537..1139
                     /locus_tag="ACG8MY_002874"
     CDS             537..1139
                     /locus_tag="ACG8MY_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0431964.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1155..1832
                     /locus_tag="ACG8MY_002875"
                     /pseudo
     CDS             1155..1832
                     /locus_tag="ACG8MY_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2143..2640
                     /locus_tag="ACG8MY_002876"
     CDS             2143..2640
                     /locus_tag="ACG8MY_002876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFQ0431965.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            2641..3057
                     /locus_tag="ACG8MY_002877"
     CDS             2641..3057
                     /locus_tag="ACG8MY_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFQ0431966.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3077..4621
                     /locus_tag="ACG8MY_002878"
     CDS             3077..4621
                     /locus_tag="ACG8MY_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0431967.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4764..4988
                     /locus_tag="ACG8MY_002879"
     CDS             4764..4988
                     /locus_tag="ACG8MY_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0431968.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5003..5872
                     /locus_tag="ACG8MY_002880"
     CDS             5003..5872
                     /locus_tag="ACG8MY_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0431969.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5853..6587
                     /locus_tag="ACG8MY_002881"
     CDS             5853..6587
                     /locus_tag="ACG8MY_002881"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0431970.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6620..7528
                     /locus_tag="ACG8MY_002882"
     CDS             6620..7528
                     /locus_tag="ACG8MY_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0431971.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7525..8067
                     /gene="sat4"
                     /locus_tag="ACG8MY_002883"
     CDS             7525..8067
                     /gene="sat4"
                     /locus_tag="ACG8MY_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFQ0431972.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8160..8954
                     /locus_tag="ACG8MY_002884"
                     /note="aph(3')-IIIa"
     CDS             8160..8954
                     /locus_tag="ACG8MY_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFQ0431973.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9230..9361
                     /locus_tag="ACG8MY_002885"
                     /pseudo
     CDS             9230..9361
                     /locus_tag="ACG8MY_002885"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            9437..9930
                     /locus_tag="ACG8MY_002886"
                     /pseudo
     CDS             9437..9930
                     /locus_tag="ACG8MY_002886"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         3530 a         1562 c         2203 g         2746 t
ORIGIN      
        1 acaagaagca ttttattcta gcttaatttg acaaatcata ctatgatacg tgtttgtttt
       61 tctgtgctgt ttagtgaatg attagcagaa atatagagag taagatttta attaattatt
      121 agggggagaa ggagagagta gcccgaaaac ttttagttgg cttggactga acgaagtgag
      181 ggaaaggcta ctaaaacgtc gaggggcagt gagagcgaag cgaacacttg atcttttaag
      241 ttgctatctt tttaaggtca atagagtata cttatttgtc ctaatatggt tttagcagta
      301 taattgactt ggtgaatagg tcatttaagt tgggcataat aggaggagta aaatgaaaaa
      361 atttatttat cgagttttag aaaatgacga agtggtggct atttttaatg agcaacaata
      421 tgcgcaagat tttatcgctt acgaaaagac aatttctgat aagcaatttg aaattgaaaa
      481 agtagatata gctgattggt tattgcaacc gagagaattt tagaggttgg ttgaaaatgg
      541 ctaaaattgg ttatgcacgt gtcagtagca aagaacagaa cttagataga caactgaaag
      601 cgttagaggg cgtttctaag gtcttttcag acaaattaag cggtcaatcg gtcgaacgcc
      661 cacaattaca agctatgctt aactatattc gtgaagggga tatagttgtt gttactgaat
      721 tagatcgatt aggacgaaat aataaagaat taacagaatt gatgaatcaa attcaaatta
      781 agggggcaac cctggaagtc ttaaatttac cctcaatgaa tggtattgaa gatgaaaatt
      841 taagacggct gattaataat ttagtgattg aattgtataa gtaccaagcg gaatctgaac
      901 gcaaacgaat taaagaacgc caagcccaag gaattgaaat tgctaagaaa aaaggaaaat
      961 tcaaagggcg acaacctaaa ttcaaagaaa atgatccacg tttacaacac gctttcgatt
     1021 tatttttgaa tggttgttcc gataaagaag ttgaagaaca aactggaatt aatcgccgaa
     1081 cgtttagaag gtatcgatca agatacaacg tgacagtaga tcaaagaaaa aaacaatgaa
     1141 aagagggata gttaatgagt acggttattt tagctgaaaa accaagccag gcattagcct
     1201 acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta ttttgagatc aaagacccac
     1261 tatttacaga tgaaacgttt atcacctttg gttttgggca tttagtcgaa ttagcagaac
     1321 caggtcatta taacgaaaag tggcagaact ggaaacttga atctttgccg atttttcctg
     1381 atcgatacga ttttgaagtt gcaaaagata agggaaagca gtttaaaatt gttgctgaac
     1441 ttctcaaaaa ggcaaataca attattgtcg caacagatag cgacagagaa ggtgaaaata
     1501 tcgcctggtc gattatccat aaagcaaatg ccttttctaa agataaaacg tataaaagat
     1561 tatggatcaa tagcttagaa aaagatgtga tccgaagcgg ttttcaaaat ttgcaaccag
     1621 gaatgaatta ctatcccttt tatcaagaag cgcaaacacg ccaaattgcc gattggttga
     1681 tcggcatgaa cgcaagccct ttatatacct tgaatttaca acaaaaaggc gtacaaggta
     1741 cattttcatt aggacgtgta caaacaccga ctttatatct catttatcaa cgccaggaag
     1801 caatagaaaa ctttaaaaaa gaaccttttt tcctgaataa ttcatagttt tactaatagt
     1861 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
     1921 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
     1981 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
     2041 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
     2101 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
     2161 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
     2221 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
     2281 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
     2341 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
     2401 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
     2461 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
     2521 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
     2581 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
     2641 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
     2701 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
     2761 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
     2821 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     2881 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     2941 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     3001 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     3061 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     3121 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     3181 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     3241 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     3301 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     3361 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     3421 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     3481 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     3541 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     3601 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     3661 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     3721 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     3781 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     3841 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     3901 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     3961 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     4021 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     4081 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     4141 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     4201 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     4261 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     4321 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     4381 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     4441 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     4501 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     4561 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     4621 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     4681 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     4741 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     4801 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     4861 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     4921 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     4981 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     5041 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     5101 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     5161 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
     5221 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
     5281 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
     5341 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
     5401 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     5461 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     5521 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
     5581 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
     5641 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
     5701 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
     5761 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     5821 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     5881 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     5941 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     6001 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     6061 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     6121 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     6181 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     6241 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     6301 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     6361 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     6421 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     6481 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     6541 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     6601 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     6661 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     6721 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     6781 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     6841 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     6901 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     6961 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     7021 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     7081 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     7141 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     7201 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     7261 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     7321 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     7381 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     7441 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     7501 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     7561 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     7621 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     7681 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     7741 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     7801 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     7861 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     7921 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     7981 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     8041 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     8101 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     8161 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     8221 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     8281 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     8341 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     8401 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     8461 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     8521 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     8581 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     8641 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     8701 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     8761 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     8821 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     8881 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     8941 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     9001 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     9061 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     9121 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     9181 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     9241 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     9301 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     9361 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     9421 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     9481 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     9541 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     9601 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     9661 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     9721 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     9781 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     9841 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     9901 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     9961 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
    10021 cttttatata ttaatcacaa a
//
LOCUS       DAZKNO010000397         6241 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.146, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000397 DAZKNO010000000
VERSION     DAZKNO010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.146"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..659
                     /gene="vanR"
                     /locus_tag="ACG8MY_002887"
     CDS             3..659
                     /gene="vanR"
                     /locus_tag="ACG8MY_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HFQ0431974.1"
                     /translation="MADLVELYLKNENYTVFKYYTAKEALECIDKSEIDLAILDIMLP
                     GTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITKPFRPLELIARVKA
                     QLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPTEFSILRILCENKG
                     NVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKYIKTVWGVGYKIEK
                     "
     gene            637..1791
                     /locus_tag="ACG8MY_002888"
     CDS             637..1791
                     /locus_tag="ACG8MY_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0431975.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2006..2974
                     /locus_tag="ACG8MY_002889"
     CDS             2006..2974
                     /locus_tag="ACG8MY_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0431976.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            2967..3998
                     /gene="vanA"
                     /locus_tag="ACG8MY_002890"
     CDS             2967..3998
                     /gene="vanA"
                     /locus_tag="ACG8MY_002890"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0431977.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAFITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4004..4612
                     /locus_tag="ACG8MY_002891"
     CDS             4004..4612
                     /locus_tag="ACG8MY_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0431978.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            5040..5951
                     /locus_tag="ACG8MY_002892"
     CDS             5040..5951
                     /locus_tag="ACG8MY_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0431979.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1989 a         1134 c         1414 g         1704 t
ORIGIN      
        1 aaattgccga tttggttgaa ttatacttaa aaaacgagaa ttatacggtt ttcaaatact
       61 ataccgccaa agaagcattg gaatgtatag acaagtctga gattgacctt gccatattgg
      121 acatcatgct tcccggcaca agcggcctta ctatctgtca aaaaataagg gacaagcaca
      181 cctatccgat tatcatgctg accgggaaag atacagaggt agataaaatt acagggttaa
      241 caatcggcgc ggatgattat ataacgaagc cctttcgccc actggagtta attgctcggg
      301 taaaggccca gttgcgccga tacaaaaaat tcagtggagt aaaggagcag aacgaaaatg
      361 ttatcgtcca ctccggcctt gtcattaatg ttaacaccca tgagtgttat ctgaacgaga
      421 agcagttatc ccttactccc accgagtttt caatactgcg aatcctctgt gaaaacaagg
      481 ggaatgtggt tagctccgag ctgctatttc atgagatatg gggcgacgaa tatttcagca
      541 agagcaacaa caccatcacc gtgcatatcc ggcatttgcg cgaaaaaatg aacgacacca
      601 ttgataatcc gaaatatata aaaacggtat ggggggttgg ttataaaatt gaaaaataaa
      661 aaaaacgact attccaaact agaacgaaaa ctttacatgt atatcgttgc aattgttgtg
      721 gtagcaattg tattcgtgtt gtatattcgt tcaatgatcc gagggaaact tggggattgg
      781 atcttaagta ttttggaaaa caaatatgac ttaaatcacc tggacgcgat gaaattatat
      841 caatattcca tacggaacaa tatagatatc tttatttatg tggcgattgt cattagtatt
      901 cttattctat gtcgcgtcat gctttcaaaa ttcgcaaaat actttgacga gataaatacc
      961 ggcattgatg tacttattca gaacgaagat aaacaaattg agctttctgc ggaaatggat
     1021 gttatggaac aaaagctcaa cacattaaaa cggactctgg aaaagcgaga gcaggatgca
     1081 aagctggccg aacaaagaaa aaatgacgtt gttatgtact tggcgcacga tattaaaacg
     1141 ccccttacat ccattatcgg ttatttgagc ctgcttgacg aggctccaga catgccggta
     1201 gatcaaaagg caaagtatgt gcatatcacg ttggacaaag cgtatcgact cgaacagcta
     1261 atcgacgagt tttttgagat tacacggtat aacctacaaa cgataacgct aacaaaaacg
     1321 cacatagacc tatactatat gctggtgcag atgaccgatg aattttatcc tcagctttcc
     1381 gcacatggaa aacaggcggt tattcacgcc cccgaggatc tgaccgtgtc cggcgaccct
     1441 gataaactcg cgagagtctt taacaacatt ttgaaaaacg ccgctgcata cagtgaggat
     1501 aacagcatca ttgacattac cgcgggcctc tccggggatg tggtgtcaat cgaattcaag
     1561 aacactggaa gcatcccaaa agataagcta gctgccatat ttgaaaagtt ctataggctg
     1621 gacaatgctc gttcttccga tacgggtggc gcgggacttg gattggcgat tgcaaaagaa
     1681 attattgttc agcatggagg gcagatttac gcggaaagca atgataacta tacgacgttt
     1741 agggtagagc ttccagcgat gccagacttg gttgataaaa ggaggtccta agagatgtat
     1801 ataatttttt aggaaaatct caaggttatc tttacttttt cttaggaaat taacaattta
     1861 atattaagaa acggctcgtt cttacacggt agacttaata ccgtaagaac gagccgtttt
     1921 cgttcttcag agaaagattt gacaagatta ccattggcat ccccgtttta tttggtgcct
     1981 ttcacagaaa gggttggtct taattatgaa taacatcggc attactgttt atggatgtga
     2041 gcaggatgag gcagatgcat tccatgctct ttcgcctcgc tttggcgtta tggcaacgat
     2101 aattaacgcc aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc aatgtatcag
     2161 tgtgggacat aaatcagaga tttccgcctc tattcttctt gcgctgaaga gagccggtgt
     2221 gaaatatatt tctacccgaa gcatcggctg caatcatata gatacaactg ctgctaagag
     2281 aatgggcatc actgtcgaca atgtggcgta ctcgccggat agcgttgccg attatactat
     2341 gatgctaatt cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca
     2401 tgatttcagg ttggacagcg accgtggcaa ggtactcagc gacatgacag ttggtgtggt
     2461 gggaacgggc cagataggca aagcggttat tgagcggctg cgaggatttg gatgtaaagt
     2521 gttggcttat agtcgcagcc gaagtataga ggtaaactat gtaccgtttg atgagttgct
     2581 gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat acggatacgc actatattat
     2641 cagccacgaa caaatacaga gaatgaagca aggagcattt cttatcaata ctgggcgcgg
     2701 tccacttgta gatacctatg agttggttaa agcattagaa aacgggaaac tgggcggtgc
     2761 cgcattggat gtattggaag gagaggaaga gtttttctac tctgattgca cccaaaaacc
     2821 aattgataat caatttttac ttaaacttca aagaatgcct aacgtgataa tcacaccgca
     2881 tacggcctat tataccgagc aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg
     2941 tttggatttt gaaaggagac aggagcatga atagaataaa agttgcaata ctgtttgggg
     3001 gttgctcaga ggagcatgac gtatcggtaa aatctgcaat agagatagcc gctaacatta
     3061 ataaagaaaa atacgagccg ttatacattg gaattacgaa atctggtgta tggaaaatgt
     3121 gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta ctctcgccgg
     3181 ataaaaaaat gcacggatta cttgttaaaa agaaccatga atatgaaatc aaccatgttg
     3241 atgtagcatt ttcagctttg catggcaagt caggtgaaga tggatccata caaggtctgt
     3301 ttgaattgtc cggtatccct tttgtaggct gcgatattca aagctcagca atttgtatgg
     3361 acaaatcgtt gacatacatc gttgcgaaaa atgctgggat agctactccc gccttttggg
     3421 ttattaataa agatgatagg ccggtggcag ctacgtttac ctatcctgtt tttgttaagc
     3481 cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac gaattggact
     3541 acgcaattga atcggcaaga caatatgaca gcaaaatctt aattgagcag gctgtttcgg
     3601 gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg
     3661 accaaatcag gctgcagtac ggaatctttc gtattcatca ggaagtcgag ccggaaaaag
     3721 gctctgaaaa cgcatttata accgttcccg cagacctttc agcagaggag cgaggacgga
     3781 tacaggaaac ggcaaaaaaa atatataaag cgctcggctg tagaggtcta gcccgtgtgg
     3841 atatgttttt acaagataac ggccgcattg tactgaacga agtcaatact ctgcccggtt
     3901 tcacgtcata cagtcgttat ccccgtatga tggccgctgc aggtattgca cttcccgaac
     3961 tgattgaccg cttgatcgta ttagcgttaa aggggtgata agcatggaaa taggatttac
     4021 ttttttagat gaaatagtac acggtgttcg ttgggacgct aaatatgcca cttgggataa
     4081 tttcaccgga aaaccggttg acggttatga agtaaatcgc attgtaggga catacgagtt
     4141 ggctgaatcg cttttgaagg caaaagaact ggctgctacc caagggtacg gattgcttct
     4201 atgggacggt taccgtccta agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc
     4261 ggaaaataac ctgacaaagg aaagttatta tcccaatatt gaccgaactg agatgatttc
     4321 aaaaggatac gtggcttcaa aatcaagcca tagccgcggc agtgccattg atcttacgct
     4381 ttatcgatta gacacgggtg agcttgtacc aatggggagc cgatttgatt ttatggatga
     4441 acgctctcat catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt
     4501 gcgctccatc atggaaaaca gtgggtttga agcatatagc ctcgaatggt ggcactatgt
     4561 attaagagac gaaccatacc ccaatagcta ttttgatttc cccgttaaat aaacttttaa
     4621 ccgttgcacg gacaaactat ataagctaac tctttcggca ggaaacccga cgtatgtaac
     4681 tggttcttag ggaatttata tatagtagat agtattgaag atgtaaggca gagcgatatt
     4741 gcggtcatta tctgcgtgcg ctgcggcaag atagcctgat aataagactg atcgcataga
     4801 ggggtggtat ttcacaccgc ccattgtcaa caggcagttc agcctcgtta aattcagcat
     4861 gggtatcact tatgaaaatt catctacatt ggtgataata gtaaatccag tagggcgaaa
     4921 taattgactg taatttacgg ggcaaaacgg cacaatctca aacgagattg tgccgtttaa
     4981 ggggaagatt ctagaaatat ttcatacttc caactatata gttaaggagg agactgaaaa
     5041 tgaagaagtt gtttttttta ttgttattgt tattcttaat atacttaggt tatgactacg
     5101 ttaatgaagc actgttttct caggaaaaag tcgaatttca aaattatgat caaaatccca
     5161 aagaacattt agaaaatagt gggacttctg aaaataccca agagaaaaca attacagaag
     5221 aacaggttta tcaaggaaat ctgctattaa tcaatagtaa atatcctgtt cgccaagaaa
     5281 gtgtgaagtc agatatcgtg aatttatcta aacatgacga attaataaat ggatacgggt
     5341 tgcttgatag taatatttat atgtcaaaag aaatagcaca aaaattttca gagatggtca
     5401 atgatgctgt aaagggtggc gttagtcatt ttattattaa tagtggctat cgagactttg
     5461 atgagcaaag tgtgctttac caagaaatgg gggctgagta tgccttacca gcaggttata
     5521 gtgagcataa ttcaggttta tcactagatg taggatcaag cttgacgaaa atggaacgag
     5581 cccctgaagg aaagtggata gaagaaaatg cttggaaata cgggttcatt ttacgttatc
     5641 cagaggacaa aacagagtta acaggaattc aatatgaacc atggcatatt cgctatgttg
     5701 gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt tctcgaggaa tatatggatt
     5761 acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg ggaaaaatat gagatctttt
     5821 attatcctgt tactaaaaat accaccattc atgtgccgac taatcttcgt tatgagatat
     5881 caggaaacaa tatagacggt gtaattgtga cagtgtttcc cggatcaaca catactaatt
     5941 caaggaggta aggatggcgg aatgaaacca acgaaattaa tgaacagcat tattgtacta
     6001 gcacttttgg ggtaacgtta gctttttaat ttaaaaccca cgttaactag gacattgcta
     6061 tactaatgat acaacttaaa caaaagaatt agaggaaatt atattgggaa aaatattatc
     6121 tagaggattg ctagctttat atttagtgac actaatctgg ttagtgttat tcaaattaca
     6181 atacaatatt ttatcagtat ttaattatca tcaaagaagt cttaacttga ctccatttac
     6241 t
//
LOCUS       DAZKNO010000398         5415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.160, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000398 DAZKNO010000000
VERSION     DAZKNO010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.160"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..668
                     /locus_tag="ACG8MY_002893"
     CDS             <1..668
                     /locus_tag="ACG8MY_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0431980.1"
                     /translation="FWNHFKAEHEAMLKQAQDEPAIDEIYHFDFLEAKELGLKHNSSF
                     YSKHMDFSSSEPKTDLFFGATNNGRKERAESYKQEFIKRDVTTNYFILPHRGNEQPGY
                     LAYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRPEN
                     IFLLRERDLDELPEFLHTPFKPIPSELLEFYDAENWAKRFITNDSTIYEQYEYDLSSV
                     ENK"
     gene            complement(818..1759)
                     /locus_tag="ACG8MY_002894"
     CDS             complement(818..1759)
                     /locus_tag="ACG8MY_002894"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0431981.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFRSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKTAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            1884..3005
                     /locus_tag="ACG8MY_002895"
     CDS             1884..3005
                     /locus_tag="ACG8MY_002895"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0431982.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPHGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3263..4765
                     /gene="eat(A)"
                     /locus_tag="ACG8MY_002896"
     CDS             3263..4765
                     /gene="eat(A)"
                     /locus_tag="ACG8MY_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0431983.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKSQPKHLSISYVRQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4832..5359
                     /locus_tag="ACG8MY_002897"
     CDS             4832..5359
                     /locus_tag="ACG8MY_002897"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0431984.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1764 a          973 c         1152 g         1526 t
ORIGIN      
        1 ttttttggaa tcacttcaaa gcagaacacg aggcgatgct gaagcaggca caagatgaac
       61 cggcaatcga tgagatttat cattttgatt ttttggaagc aaaagaactt gggctgaaac
      121 ataatagttc attttattcg aaacatatgg atttttcttc tagcgaacca aagacggatc
      181 tattttttgg tgcaacgaat aatgggagaa aagaacgagc agaaagctat aagcaggaat
      241 ttataaaaag agatgttacg accaactatt tcattcttcc acatcgaggg aatgaacaac
      301 ctggatatct tgcttatgaa gagtacctga aaaaaacagg ggaatcgcgc gggattctgg
      361 aactgttgcg ggagggtcag caaggagtga ctttgcggac atttgaatct atgtattttc
      421 aaaagaaact agtgacggat aatcaagcga ttgctcatta tcatttttat cgtcctgaaa
      481 atattttttt gcttcgagaa cgtgatttag atgaattacc ggaatttctg catacacctt
      541 ttaagccaat cccatcagag ctattggaat tttatgatgc agaaaattgg gcaaaacgat
      601 ttatcactaa cgattccact atttatgagc aatacgaata tgacctctcc tctgttgaaa
      661 ataaataaaa aacctacatt ttgaagagat tatgtaccga aacaaacaag cacaattctt
      721 atggcatcat gagagttgtg ttttcttttt gatctttcat tgggcttaaa aaaagagaac
      781 cagctgctga ttcagctgat tctcatcaga gagcctttta ttctgcgcca aggattcctt
      841 cttttgcatg gactaattgg gtcaaaaagg cacaatatgg tgtagcaata tcatattttt
      901 gtccttttcg ccaaacggca ccattgatgt agtcgatttc agtcaaacga tggtttttga
      961 tcaggtcttg gtacatagat gggtaatgca acccgatgtt gtcaggatca taagtagaag
     1021 caatatgttg gtacacttct tcttgatcta atatgattcc ttcttttgct gcgatagaag
     1081 caaattcaga tacgattgtt cgaaccatgc tctctgccgt tttttgcgcg cctagttcag
     1141 caatattaca atccaaaatc gtgcataaac cattcaatgt tccatttaca caagcttttc
     1201 gccaaatgga ataacgaaca ttatggctat agatcggatt taattttgct tcttgaaaga
     1261 cttccacaac tttttttgta aatgcttcgc cttctggttc caagttctct agttcgattt
     1321 ctccatcacc taacaatgtg acttttccgg gaccagctag accagctgtc cacatcgtga
     1381 tcccaaataa gatatttttc tttggcacat atttttccag tacatcttca tgacctaatc
     1441 catttagtaa acacaaaaca taagtattgt cagtaataat ggattggatc gaacgaaaca
     1501 tatcatctaa ttgattcgct tttgttaatg ctacgatcaa gtccacgtgt tcgttgcttt
     1561 cgatgatttc ttctggtgaa tagattggca gttttgcgac cacttcttta ccgttaaagt
     1621 cagcaatcaa cccatttgta cgaatggctt caatgtgtgc aggccaacga tcgatcaaga
     1681 ggacctcatt tccgctttga tgtagcatca atccgattcg gctgcccatt gctccggctc
     1741 cagctattgc gattttcatg ttttttctct cctttatcaa tgagttccct tttagtatat
     1801 agaaaaatgg aagaaaatag cattgttttg tttgaaaaaa atgaaaaaaa gtgtcttact
     1861 taagaagaaa gtgagggata cagatggata ttttggcggc aattgattca tttaaaggaa
     1921 gtgcttcttc atttgaacta aatcaggcag ttctaaatgg ctgggaagat cgtaaaggaa
     1981 gaaaaatcaa tgtgccgatt gcagatggcg gagaaaatac tgcagaagca atctattacg
     2041 cgctcggggg aacatggcgc accattgatg ggatggatct tttattccgt cagcgaaaaa
     2101 tcagctattt attgacaact tggcaaggaa aaaataccgc agtcatcgaa tctgcacaag
     2161 cattggggtt agatctactt gataaaccaa cggatcaaac tatccgactt gcttccagtt
     2221 atgccttagg agaacttatc aaagatgcac tgcttcaaga agtagaacaa attattgtca
     2281 ctttaggagg aagcgggtgt agcgatggtg gtctaggatt actccaatcc ttgggtgctt
     2341 ctttagaagg gtacacagaa ggaaatcctt tattatctac taaaaaactt cattttgatc
     2401 gaagaaacgt tcttgtttct aaagtaaaat tgattatcgc tgcagatgtt accaatccat
     2461 atacaggtca acaaggagct gcttggcaat ttggaagaca aaaaggagga atagatacga
     2521 cgctagcttt cttagataaa caagcaggaa agattgctca gcaaataaaa gatttctggg
     2581 aggtggattt agatgatatt ccaggaagtg gagcagcagg cggtattggt ggagcccttt
     2641 ttttgttggg agcgaagatg gtttctggtt tcgatttagt cagccaaatc actggattgg
     2701 aaaaaaagat cgaacaagca gatgtcatca ttacaggaga agggcggatc gatcagcaaa
     2761 cgatccacgg aaaagttcca cacggtgtag cagtgcttgc aaaaaaatat cgaaaaccaa
     2821 taatcgctat ttgcggaagt agagaccaag agctagaagg actctctgat tttctcccaa
     2881 ttgtcttaag tattcatttg ggtccaatag gtttagaaga agcaatggaa cacaggcaaa
     2941 cattagaaaa aacaaaaatc ttaggacaga cacttagcag atttttcacc atcctcccga
     3001 agtagttgac aaaatcattt tctttatgct atcgtaattt caaatatagg acaaagtgag
     3061 ggatccgtgc attgaaaaat taagtcgatt gatgaaagct gcattcagac tgtaccttgt
     3121 ggagacaagg aagcagtgtg cagattttcg ggattggctt ggttttttcg atgtatgggt
     3181 ctttttgtgt gtatccaacc cttgcatcct ctttagccag ttctgaaaat aggactggtc
     3241 tttttttacg aggtgatgga aaatgtctaa aatcgaaata aaaaatctga cattcggcta
     3301 cgacagccaa ggcacattat tatttgaaca agcaaatcta aattttgaca cacaatggaa
     3361 actaggactt atcggacgaa acggtcgagg aaagacaact ttactgaata ttctacaaaa
     3421 caaactacct tatcaagggc aagtaatcca tcagcaagaa tttgcctatt tcccgcaaca
     3481 gacaaaagat aaagaacgtt taacctatta cgtgttaaat gatattacgg attttgagat
     3541 atgggaaatc gaaagagagc tccaattgat gcaaacagat cctgaaatct tatggagaga
     3601 attcagcaca ctatcggggg gagagaagac aaaagtccta ctggcacttt tatttgtgga
     3661 tgacactcat ttcccgttaa tcgatgaacc aacgaatcat ttggatatct ctggtagaaa
     3721 acaagtagcg gcttatttga aaaagaaaaa acaaggcttc atcgtggtca gccatgaccg
     3781 gggatttatc gatgaagtag tggaccatgt tttagcaatc gaaaaaagtc aactggaact
     3841 ttatcaaggg aatttctcta tctatgaaga acagaaaaaa cttcgtgatg aatttgaaat
     3901 ggctcaaaat gaaaaattga aaaaagaagt cagtaggcta aagaaaacag cagctgaaaa
     3961 agccgaatgg tctcgttccc gagaaggaga taaaacaaag aaacaagtcg gattcatcga
     4021 tactgaatct agacgagtga ataaaggagc agtgggtgct gatgctgcac ggacgatgaa
     4081 acgatccaaa gcaatcgtga atcggatgga gacccagatc agcgagaaag aaaaactatt
     4141 aaaagatatc gaatatatcg attctttgac gatgaatagc caagcgtctc accataagcg
     4201 acttttaagc gtagaagatc ttcaattagg gtatgaaaat ctgttattcg agccaattca
     4261 ttttacaatc gagcctcatc agcgggtggc gatttcaggt cctaacggtg caggaaagtc
     4321 atccattatc cattatcttc tgggggcatt caacggcaag gttataggag aaaaatccca
     4381 gccaaaacat ctgagcatta gttatgtacg ccaaaattat gaagacaatc gaggaacgtt
     4441 ggcggaattt gcagagaaaa accaagtaga ctaccaagca tttttgaaca acctccgaaa
     4501 gcttgggatg gaaagagatg tttttcataa caagatcgag cagatgagta tgggccaacg
     4561 gaaaaaagtg gaattggcta aatctttatc acagccagct gaactatata catgggatga
     4621 accattgaat tatttggatg tcttcaatca agaacaatta gaacaactga tcttgaacgt
     4681 gaaacctgcc atgttactag tggaacatga tcaaaccttc ctggataaag tatctactga
     4741 gattatttct cttgagagaa tctaaccctt gtaatctcaa gaacaacaag gtatggttaa
     4801 ggggaacttt gccaacgaag gagagaatca catgctttat gtaacgagac acggagaaac
     4861 aacttggaac gcacaaggat tagtttgcgg acgggcagat gtgccgctga ctgaaaaagg
     4921 acagatgcag gcacagaaac tagcagaaaa ggtagtagat ttgcctgttc caatcacgaa
     4981 aatcatccat tcccctcttc aacgagcaag agatacagca caagcagttg ctgatcgtct
     5041 cagcttgccg ctgaccgttg atgaacgatt ggtagaaatg gattttgggg attatgatgg
     5101 actgccaagc aaagatgaga actttcaaaa agcacgttta gcgttcgctg tacgattccc
     5161 aaatggggag tcagtcttag atgtgtatgc acggatcgtt cctttattga aagaatgcat
     5221 cgaagacgaa gaaaatgtct atctgttagt ttgccataat gccttgataa gagtgatcaa
     5281 tgcttatttt catccgatgc ctaatgaggg gttctttaca ttcatggtag ataatacaga
     5341 attggtttct tatgagtaat tatgtttttg tgttatttaa ataaaatatt tcaaggcact
     5401 gcttttgctt agcag
//
LOCUS       DAZKNO010000399         5153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.166, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000399 DAZKNO010000000
VERSION     DAZKNO010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.166"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..700
                     /gene="vanR-A"
                     /locus_tag="ACG8MY_002898"
     CDS             5..700
                     /gene="vanR-A"
                     /locus_tag="ACG8MY_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0431985.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            678..1832
                     /locus_tag="ACG8MY_002899"
     CDS             678..1832
                     /locus_tag="ACG8MY_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0431986.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2047..3015
                     /locus_tag="ACG8MY_002900"
     CDS             2047..3015
                     /locus_tag="ACG8MY_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0431987.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3008..4039
                     /gene="vanA"
                     /locus_tag="ACG8MY_002901"
     CDS             3008..4039
                     /gene="vanA"
                     /locus_tag="ACG8MY_002901"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0431988.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAFITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4045..4653
                     /locus_tag="ACG8MY_002902"
     CDS             4045..4653
                     /locus_tag="ACG8MY_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0431989.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1589 a          979 c         1204 g         1381 t
ORIGIN      
        1 aactatgagc gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga
       61 attatactta aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt
      121 ggaatgtata gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac
      181 aagcggcctt actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct
      241 gaccgggaaa gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta
      301 tataacgaag ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg
      361 atacaaaaaa ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct
      421 tgtcattaat gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc
      481 caccgagttt tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga
      541 gctgctattt catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac
      601 cgtgcatatc cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat
      661 aaaaacggta tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac
      721 tagaacgaaa actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt
      781 tgtatattcg ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa
      841 acaaatatga cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca
      901 atatagatat ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca
      961 tgctttcaaa attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc
     1021 agaacgaaga taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca
     1081 acacattaaa acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa
     1141 aaaatgacgt tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg
     1201 gttatttgag cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg
     1261 tgcatatcac gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga
     1321 ttacacggta taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata
     1381 tgctggtgca gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg
     1441 ttattcacgc ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct
     1501 ttaacaacat tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta
     1561 ccgcgggcct ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa
     1621 aagataagct agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg
     1681 atacgggtgg cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag
     1741 ggcagattta cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga
     1801 tgccagactt ggttgataaa aggaggtcct aagagatgta tataattttt taggaaaatc
     1861 tcaaggttat ctttactttt tcttaggaaa ttaacaattt aatattaaga aacggctcgt
     1921 tcttacacgg tagacttaat accgtaagaa cgagccgttt tcgttcttca gagaaagatt
     1981 tgacaagatt accattggca tccccgtttt atttggtgcc tttcacagaa agggttggtc
     2041 ttaattatga ataacatcgg cattactgtt tatggatgtg agcaggatga ggcagatgca
     2101 ttccatgctc tttcgcctcg ctttggcgtt atggcaacga taattaacgc caacgtgtcg
     2161 gaatccaacg ccaaatccgc gcctttcaat caatgtatca gtgtgggaca taaatcagag
     2221 atttccgcct ctattcttct tgcgctgaag agagccggtg tgaaatatat ttctacccga
     2281 agcatcggct gcaatcatat agatacaact gctgctaaga gaatgggcat cactgtcgac
     2341 aatgtggcgt actcgccgga tagcgttgcc gattatacta tgatgctaat tcttatggca
     2401 gtacgcaacg taaaatcgat tgtgcgctct gtggaaaaac atgatttcag gttggacagc
     2461 gaccgtggca aggtactcag cgacatgaca gttggtgtgg tgggaacggg ccagataggc
     2521 aaagcggtta ttgagcggct gcgaggattt ggatgtaaag tgttggctta tagtcgcagc
     2581 cgaagtatag aggtaaacta tgtaccgttt gatgagttgc tgcaaaatag cgatatcgtt
     2641 acgcttcatg tgccgctcaa tacggatacg cactatatta tcagccacga acaaatacag
     2701 agaatgaagc aaggagcatt tcttatcaat actgggcgcg gtccacttgt agatacctat
     2761 gagttggtta aagcattaga aaacgggaaa ctgggcggtg ccgcattgga tgtattggaa
     2821 ggagaggaag agtttttcta ctctgattgc acccaaaaac caattgataa tcaattttta
     2881 cttaaacttc aaagaatgcc taacgtgata atcacaccgc atacggccta ttataccgag
     2941 caagcgttgc gtgataccgt tgaaaaaacc attaaaaact gtttggattt tgaaaggaga
     3001 caggagcatg aatagaataa aagttgcaat actgtttggg ggttgctcag aggagcatga
     3061 cgtatcggta aaatctgcaa tagagatagc cgctaacatt aataaagaaa aatacgagcc
     3121 gttatacatt ggaattacga aatctggtgt atggaaaatg tgcgaaaaac cttgcgcgga
     3181 atgggaaaac gacaattgct attcagctgt actctcgccg gataaaaaaa tgcacggatt
     3241 acttgttaaa aagaaccatg aatatgaaat caaccatgtt gatgtagcat tttcagcttt
     3301 gcatggcaag tcaggtgaag atggatccat acaaggtctg tttgaattgt ccggtatccc
     3361 ttttgtaggc tgcgatattc aaagctcagc aatttgtatg gacaaatcgt tgacatacat
     3421 cgttgcgaaa aatgctggga tagctactcc cgccttttgg gttattaata aagatgatag
     3481 gccggtggca gctacgttta cctatcctgt ttttgttaag ccggcgcgtt caggctcatc
     3541 cttcggtgtg aaaaaagtca atagcgcgga cgaattggac tacgcaattg aatcggcaag
     3601 acaatatgac agcaaaatct taattgagca ggctgtttcg ggctgtgagg tcggttgtgc
     3661 ggtattggga aacagtgccg cgttagttgt tggcgaggtg gaccaaatca ggctgcagta
     3721 cggaatcttt cgtattcatc aggaagtcga gccggaaaaa ggctctgaaa acgcatttat
     3781 aaccgttccc gcagaccttt cagcagagga gcgaggacgg atacaggaaa cggcaaaaaa
     3841 aatatataaa gcgctcggct gtagaggtct agcccgtgtg gatatgtttt tacaagataa
     3901 cggccgcatt gtactgaacg aagtcaatac tctgcccggt ttcacgtcat acagtcgtta
     3961 tccccgtatg atggccgctg caggtattgc acttcccgaa ctgattgacc gcttgatcgt
     4021 attagcgtta aaggggtgat aagcatggaa ataggattta cttttttaga tgaaatagta
     4081 cacggtgttc gttgggacgc taaatatgcc acttgggata atttcaccgg aaaaccggtt
     4141 gacggttatg aagtaaatcg cattgtaggg acatacgagt tggctgaatc gcttttgaag
     4201 gcaaaagaac tggctgctac ccaagggtac ggattgcttc tatgggacgg ttaccgtcct
     4261 aagcgtgctg taaactgttt tatgcaatgg gctgcacagc cggaaaataa cctgacaaag
     4321 gaaagttatt atcccaatat tgaccgaact gagatgattt caaaaggata cgtggcttca
     4381 aaatcaagcc atagccgcgg cagtgccatt gatcttacgc tttatcgatt agacacgggt
     4441 gagcttgtac caatggggag ccgatttgat tttatggatg aacgctctca tcatgcggca
     4501 aatggaatat catgcaatga agcgcaaaat cgcagacgtt tgcgctccat catggaaaac
     4561 agtgggtttg aagcatatag cctcgaatgg tggcactatg tattaagaga cgaaccatac
     4621 cccaatagct attttgattt ccccgttaaa taaactttta accgttgcac ggacaaacta
     4681 tataagctaa ctctttcggc aggaaacccg acgtatgtaa ctggttctta gggaatttat
     4741 atatagtaga tagtattgaa gatgtaaggc agagcgatat tgcggtcatt atctgcgtgc
     4801 gctgcggcaa gatagcctga taataagact gatcgcatag aggggtggta tttcacaccg
     4861 cccattgtca acaggcagtt cagcctcgtt aaattcagca tgggtatcac ttatgaaaat
     4921 tcatctacat tggtgataat agtaaatcca gtagggcgaa ataattgact gtaatttacg
     4981 gggcaaaacg gcacaatctc aaacgagatt gtgccgttta aggggaagat tctagaaata
     5041 tttcatactt ccaactatat agttaaggag gagactgaaa atgaagaagt tgtttttttt
     5101 attgttattg ttattcttaa tatacttagg ttatgactac gttaatgaag cac
//
LOCUS       DAZKNO010000400         2770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.221, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000400 DAZKNO010000000
VERSION     DAZKNO010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.221"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..192
                     /locus_tag="ACG8MY_002903"
     CDS             109..192
                     /locus_tag="ACG8MY_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFQ0431990.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="ACG8MY_002904"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="ACG8MY_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0431991.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1317..2180)
                     /locus_tag="ACG8MY_002905"
     CDS             complement(1317..2180)
                     /locus_tag="ACG8MY_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0431992.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2182..2454)
                     /locus_tag="ACG8MY_002906"
     CDS             complement(2182..2454)
                     /locus_tag="ACG8MY_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0431993.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2471..2680)
                     /locus_tag="ACG8MY_002907"
     CDS             complement(2471..2680)
                     /locus_tag="ACG8MY_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0431994.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          850 a          487 c          444 g          989 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccaaaa ttttgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctacc gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttata gatgtaatca
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacac
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggaatattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tgtaaatttg
     1081 gaaagttaca cgttactaaa gggaatgtag ataaattatt aggtataggg cacctctaat
     1141 aactaccaat taattcacct caaaatgaaa ttagaaaaaa acagcgaaat ttttaaatct
     1201 atttcttatc gatacaaatt cctcgtaggc gctcgggacc ccaacatgag gaaatgtttt
     1261 tataaaaaaa gagtagttac cgttttttgg taactactct tttccttttt taaggtttaa
     1321 atacctggaa gtttaggtgt tttgggtatt ggtggtgtag gtagctgcaa gctttccatt
     1381 ttttgttgaa ttgctttaaa ttcaggggtt tcttggtgtt gattttgaac cattttttgc
     1441 tctattctct ccaaagtcgg ttgaatttct ttccctgata ctttacgatt taattctctt
     1501 tctaaggtct ctttcggact aatggaaggc gtttctaagc ttgaatagag tttcactcct
     1561 tctctattgt aaagccttat gtcgctaaat aagcccgttt tatgaagggt ttctaaattg
     1621 gtcggtaagt ttttgacaac aatatcatgc gcttgttttg gtgttgccct agctgtcatt
     1681 ggatcatctg catacatggt ttcatatcgt tcaatcgttc ctaagtatga ttcaattttc
     1741 ggtactgcca tgacgtatat ttttgtttca taacctttag attgaagcat tgtggctgtt
     1801 tgaatgggaa cgtctgttgt tcgtcctgtt ccttcaatca ctaaattata gccttgatcg
     1861 cttaaacggc taataagtgc ttctgtcatg cgattcgaat aaggggtaac gtgttttact
     1921 acgtcttttt catagagttt cactaattcg tcaaaatttg ggtgctgttg tttaaaggta
     1981 tcattatcaa tgacaataac attcccttgt gtttcttcaa aaattgctga tcgcaaactg
     2041 gttttccctg accctggttg cccaccaagt aaaaaagcgg ttggcgattc aaccgctagt
     2101 ctatttttag ttagtcgttc aacattcttt tctaaacgat cttcaaattg cttttcggtg
     2161 aagtcagtaa tattcgccat gttaagccac tttctcttta ttcaaaattt gttttgaata
     2221 ttgattcatt gaccgccaat actcatgaat agcttgtaat tcttcaaatg gttgttgaaa
     2281 taaatcaact tcttgtgcca cttctaattg atttaaaaga ttcacttcta gtaaacgagt
     2341 atttttagta tctagttcat gattcaaaac ataatttaat acccgattat aaacacttgc
     2401 cgataactca ttaatgattt caatttcaaa tgttttttca tacgtaactg ccatttcatt
     2461 ttcactcctt ttaaagtttg tcaggtaaat atttccgtat atattcttct aaggcttgat
     2521 ccataacttc ttttacgttt ccgccattct ttgctgtttc gatttttatg atatggtgca
     2581 agtcggcacg aacacgaacc gtcttatccc ccattatatc tttttttgcg ctgattggcg
     2641 tatcatttcg ttttgctttc tgtgcgccta aatttcccac gatcgctcac ttctttctat
     2701 ttcttcttac tatttatttt atcatcacct atcacaaatc acaagtgatt tgtgattaat
     2761 cacttgtgat
//
LOCUS       DAZKNO010000401         2004 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.256, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000401 DAZKNO010000000
VERSION     DAZKNO010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.256"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8MY_002908"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8MY_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0431995.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          689 a          312 c          413 g          590 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 aaaagatgaa atatttttta ataa
//
LOCUS       DAZKNO010000402         2004 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.257, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000402 DAZKNO010000000
VERSION     DAZKNO010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.257"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8MY_002909"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8MY_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0431996.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          688 a          312 c          414 g          590 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 aaaagatgaa atatttttta ataa
//
LOCUS       DAZKNO010000403         1967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.262, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000403 DAZKNO010000000
VERSION     DAZKNO010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.262"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8MY_002910"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8MY_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0431997.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          675 a          308 c          407 g          577 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatat
//
LOCUS       DAZKNO010000404         1967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.263, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000404 DAZKNO010000000
VERSION     DAZKNO010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.263"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8MY_002911"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ACG8MY_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0431998.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
BASE COUNT          674 a          308 c          408 g          577 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatat
//
LOCUS       DAZKNO010000405         1746 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.272, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000405 DAZKNO010000000
VERSION     DAZKNO010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.272"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..677
                     /gene="dfrG"
                     /locus_tag="ACG8MY_002912"
     CDS             180..677
                     /gene="dfrG"
                     /locus_tag="ACG8MY_002912"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0431999.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(749..>1746)
                     /locus_tag="ACG8MY_002913"
     CDS             complement(749..>1746)
                     /locus_tag="ACG8MY_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0432000.1"
                     /translation="YVFRSDVAYDWNISMDELNEDTRKFKEDHLNTFSRKNDRLDWSY
                     YPQEPSANDSENRNAYLHELGKITNRSRFVDGLNVDAPYTTTAHYWLIKQMVNADEWR
                     MISDDDFSIRNAFYRVFTKELRLSDAHHFICQVNKTKSRKQCLKEFGQAKAELLDWGD
                     IRGFKTKFLRTLASHYLTELLTSHQFHEEAISKDGERYRKYADNPIKHPLATKDKGFY
                     SVDCRTDLSALEPNEIAKMLLNVNDHSTNSFIQQIRRYISSLERPLTTARGDKKSYIY
                     ANFNPKYAQFAITILRTYYNFCRPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          579 a          255 c          320 g          592 t
ORIGIN      
        1 gatttataaa atgcaattga aaatgataat tgaatgaaaa tttgtgattt tgtggtatta
       61 tgataatata acttatagaa aaaattgctt attcaactaa cgggcaggat aatgagaaaa
      121 ggttttttga tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa
      181 tgaaagtttc tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca
      241 ttccttggag gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga
      301 taatattagg taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata
      361 ttattctgac gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag
      421 aagatgtttt tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga
      481 tttataattt gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat
      541 tcgaaggaga tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc
      601 aaaaagggat aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa
      661 aaaacttatt gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag
      721 agcttgggat tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa
      781 attgtttatc tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt
      841 taaaagggcg acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact
      901 tcggattgaa gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc
      961 tttcaagaga tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat
     1021 taacatttaa tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac
     1081 aatcaactga ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg
     1141 catatttgcg atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg
     1201 ttagtaattc agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc
     1261 tgatatctcc ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt
     1321 ttctgctttt cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt
     1381 ctttcgtgaa tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc
     1441 gccattcgtc ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag
     1501 gagcatcaac atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt
     1561 gaagataagc attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc
     1621 aatctaacct atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg
     1681 tatcttcatt taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca
     1741 catatc
//
LOCUS       DAZKNO010000406         1638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.280, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000406 DAZKNO010000000
VERSION     DAZKNO010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.280"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..877
                     /gene="vanY"
                     /locus_tag="ACG8MY_002914"
     CDS             <1..877
                     /gene="vanY"
                     /locus_tag="ACG8MY_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HFQ0432001.1"
                     /translation="LIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLENSGTSENTQEK
                     TITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDSNIYMSKEIAQ
                     KFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEHNSGLSLDVG
                     SSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVGLPHSAIMKE
                     KNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEISGNNIDGV
                     IVTVFPGSTHTNSRR"
     gene            1030..1515
                     /gene="vanZ-A"
                     /locus_tag="ACG8MY_002915"
     CDS             1030..1515
                     /gene="vanZ-A"
                     /locus_tag="ACG8MY_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0432002.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          584 a          224 c          310 g          520 t
ORIGIN      
        1 cttaatatac ttaggttatg actacgttaa tgaagcactg ttttctcagg aaaaagtcga
       61 atttcaaaat tatgatcaaa atcccaaaga acatttagaa aatagtggga cttctgaaaa
      121 tacccaagag aaaacaatta cagaagaaca ggtttatcaa ggaaatctgc tattaatcaa
      181 tagtaaatat cctgttcgcc aagaaagtgt gaagtcagat atcgtgaatt tatctaaaca
      241 tgacgaatta ataaatggat acgggttgct tgatagtaat atttatatgt caaaagaaat
      301 agcacaaaaa ttttcagaga tggtcaatga tgctgtaaag ggtggcgtta gtcattttat
      361 tattaatagt ggctatcgag actttgatga gcaaagtgtg ctttaccaag aaatgggggc
      421 tgagtatgcc ttaccagcag gttatagtga gcataattca ggtttatcac tagatgtagg
      481 atcaagcttg acgaaaatgg aacgagcccc tgaaggaaag tggatagaag aaaatgcttg
      541 gaaatacggg ttcattttac gttatccaga ggacaaaaca gagttaacag gaattcaata
      601 tgaaccatgg catattcgct atgttggttt accacatagt gcgattatga aagaaaagaa
      661 tttcgttctc gaggaatata tggattacct aaaagaagaa aaaaccattt ctgttagtgt
      721 aaatggggaa aaatatgaga tcttttatta tcctgttact aaaaatacca ccattcatgt
      781 gccgactaat cttcgttatg agatatcagg aaacaatata gacggtgtaa ttgtgacagt
      841 gtttcccgga tcaacacata ctaattcaag gaggtaagga tggcggaatg aaaccaacga
      901 aattaatgaa cagcattatt gtactagcac ttttggggta acgttagctt tttaatttaa
      961 aacccacgtt aactaggaca ttgctatact aatgatacaa cttaaacaaa agaattagag
     1021 gaaattatat tgggaaaaat attatctaga ggattgctag ctttatattt agtgacacta
     1081 atctggttag tgttattcaa attacaatac aatattttat cagtatttaa ttatcatcaa
     1141 agaagtctta acttgactcc atttactgct actgggaatt tcagagagat gatagataat
     1201 gttataatct ttattccatt tggcttgctt ttgaatgtca attttaaaga aatcggattt
     1261 ttacctaagt ttgcttttgt actggtttta agtcttactt ttgaaataat tcaatttatc
     1321 ttcgctattg gagcgacaga cataacagat gtaattacaa atactgttgg aggctttctt
     1381 ggactgaaat tatatggttt aagcaataag catatgaatc aaaaaaaatt agacagagtt
     1441 attatttttg taggtatact tttgctcgta ttattgctcg tttaccgtac ccatttaaga
     1501 ataaattacg tgtaagatgt ctaaatcaag caatctgatc tttcatacac ataaagatat
     1561 tgaatgaatt ggattagatg gaaaacggga tgtggggaaa cggttctgtt gcaaagtttt
     1621 aaatctacta tcaaataa
//
LOCUS       DAZKNO010000407         1496 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.288, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000407 DAZKNO010000000
VERSION     DAZKNO010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.288"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..1471
                     /gene="tet(L)"
                     /locus_tag="ACG8MY_002916"
     CDS             98..1471
                     /gene="tet(L)"
                     /locus_tag="ACG8MY_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFQ0432003.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT          427 a          213 c          300 g          556 t
ORIGIN      
        1 tgtattttat gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa
       61 taaagatttg aattaaagtg taaaggagga attaattatg aagtgtaatg aatgtaacag
      121 ggttaaatac aaccaaattt taatttggct ttgcgtttta tcttttttta gcgtattaaa
      181 tgaaatggtt ttgaacgtct cattacctga tattgcaaat gattttaata aaccacctgc
      241 gagtacaaac tgggtgaaca cagcctttat gttaaccttt tccattggaa cagctgtata
      301 tggaaagcta tctgatcaat taggcatcaa aaggttactc ctatttggaa ttataataaa
      361 ttgtttcggg tcggtaattg ggtttgttgg ccattctttc ttttccttac ttattatggc
      421 tcgttttatt caaggggctg gtgcagctgc atttccagca ctcgtaatgg ttgtagttgc
      481 gcgctatatt ccaaaggaaa ataggggtaa agcatttggt cttattggat cgatagtagc
      541 catgggagaa ggagtcggtc cagcgattgg tggaatgata gcccattata ttcattggtc
      601 ctatcttcta ctcattccta tgataacaat tatcactgtt ccgtttctta tgaaattatt
      661 aaagaaagaa gtaaggataa aaggtcattt tgatatcaaa ggaattatac taatgtctgt
      721 aggcattgta ttttttatgt tgtttacaac atcatatagc atttcttttc ttatcgttag
      781 cgtgctgtca ttcctgatat ttgtaaaaca tatcaggaaa gtaacagatc cttttgttga
      841 tcccggatta gggaaaaata taccttttat gattggagtt ctttgtgggg gaattatatt
      901 tggaacagta gcagggtttg tctctatggt tccttatatg atgaaagatg ttcaccagct
      961 aagtactgcc gaaatcggaa gtgtaattat tttccctgga acaatgagtg tcattatttt
     1021 cggctacatt ggtgggatac ttgttgatag aagaggtcct ttatacgtgt taaacatcgg
     1081 agttacattt ctttctgtta gctttttaac tgcttccttt cttttagaaa caacatcatg
     1141 gttcatgaca attataatcg tatttgtttt aggtgggctt tcgttcacca aaacagttat
     1201 atcaacaatt gtttcaagta gcttgaaaca gcaggaagct ggtgctggaa tgagtttgct
     1261 taactttacc agctttttat cagagggaac aggtattgca attgtaggtg gtttattatc
     1321 cataccctta cttgatcaaa ggttgttacc tatggaagtt gatcagtcaa cttatctgta
     1381 tagtaatttg ttattacttt tttcaggaat cattgtcatt agttggctgg ttaccttgaa
     1441 tgtatataaa cattctcaaa gggatttcta aatcgttaag ggatcaactt tgggag
//
LOCUS       DAZKNO010000408         1327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.294, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000408 DAZKNO010000000
VERSION     DAZKNO010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.294"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..>1327
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8MY_002917"
     CDS             2..>1327
                     /gene="aph(2'')-I"
                     /locus_tag="ACG8MY_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFQ0432004.1"
                     /translation="MDDDFPLMLKWLTDERVLEFYDGRDKKYTLESLKKHYTEPWEDE
                     VFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTR
                     YIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCY
                     LMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFST
                     NKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYST
                     MSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIE
                     KDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCD
                     FIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVY"
BASE COUNT          552 a          102 c          229 g          444 t
ORIGIN      
        1 aatagatgat gattttcctt tgatgttaaa atggttaact gatgaaagag tattagaatt
       61 ttatgatggt agagataaaa aatatacatt agaatcatta aaaaaacatt atacagagcc
      121 ttgggaagat gaagttttta gagtaattat tgaatataac aatgttccta ttggatatgg
      181 acaaatatat aaaatgtatg atgagttata tactgattat cattatccaa aaactgatga
      241 gatagtctat ggtatggatc aatttatagg agagccaaat tattggagta aaggaattgg
      301 tacaagatat attaaattga tttttgaatt tttgaaaaaa gaaagaaatg ctaatgcagt
      361 tattttagac cctcataaaa ataatccaag agcaataagg gcataccaaa aatctggttt
      421 tagaattatt gaagatttgc cagaacatga attacacgag ggcaaaaaag aagattgtta
      481 tttaatggaa tatagatatg atgataatgc cacaaatgtt aaggcaatga aatatttaat
      541 tgagcattac tttgataatt tcaaagtaga tagtattgaa ataatcggta gtggttatga
      601 tagtgtggca tatttagtta ataatgaata catttttaaa acaaaattta gtactaataa
      661 gaaaaaaggt tatgcaaaag aaaaagcaat atataatttt ttaaatacaa atttagaaac
      721 taatgtaaaa attcctaata ttgaatattc gtatattagt gatgaattat ctatactagg
      781 ttataaagaa attaaaggaa cttttttaac accagaaatt tattctacta tgtcagaaga
      841 agaacaaaat ttgttaaaac gagatattgc cagtttttta agacaaatgc acggtttaga
      901 ttatacagat attagtgaat gtactattga taataaacaa aatgtattag aagagtatat
      961 attgttgcgt gaaactattt ataatgattt aactgatata gaaaaagatt atatagaaag
     1021 ttttatggaa agactaaatg caacaacagt ttttgagggt aaaaagtgtt tatgccataa
     1081 tgattttagt tgtaatcatc tattgttaga tggcaataat agattaactg gaataattga
     1141 ttttggagat tctggaatta tagatgaata ttgtgatttt atatacttac ttgaagatag
     1201 tgaagaagaa ataggaacaa attttggaga agatatatta agaatgtatg gaaatataga
     1261 tattgagaaa gcaaaagaat atcaagatat agttgaagaa tattatccta ttgaaactat
     1321 tgtttat
//
LOCUS       DAZKNO010000409         1318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.296, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000409 DAZKNO010000000
VERSION     DAZKNO010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.296"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1255
                     /locus_tag="ACG8MY_002918"
                     /pseudo
     CDS             128..1255
                     /locus_tag="ACG8MY_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
BASE COUNT          440 a          199 c          253 g          426 t
ORIGIN      
        1 caattatcat tttcaattgc attttataaa tcattttgta tgtaatttgg tatgtaagtt
       61 aaaatgaaaa aagtagtcca ttttgaagta cattttgtca ttttgtactc caaaactgaa
      121 ctacttactt gcatatatac gcctttatag tggagtacta catttacgag attcggttag
      181 agtatcagaa aaagaaaaaa taaaagttac agaaatgtat acttcaataa atggtgaatt
      241 atgtaagatt gatagagctt attctggaga aattgttatt ttgcaaaatg agtttttgaa
      301 gttaaatagt gttcttggag atacaaaact attgccacag agaaaaaaga ttgaaaatcc
      361 gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct gaacagagag aaatgttgct
      421 tgatgccctt ttggaaatct cagatagtga tccgcttcta cgatattacg tggattctac
      481 gacacatgaa attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact
      541 gttgcaagaa aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga
      601 gagaccgtta aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg
      661 ggcttccatt ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag
      721 ctcggtttct cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg
      781 ctatggttgc gaacaaggat tatatggttg gaatgtgacg gattgtaaaa tctgttttaa
      841 gtatggctta tactatagcc ctgttagtac cccagcagat tttcggatgc ttgctcctat
      901 tgtattggaa caagtcttaa aaaaagctgg aacagaattg ttagagccat atcttagttt
      961 taaaatttat gcgccacagg aatatctttc acgagcatac aacgatgctc ctaaatattg
     1021 tgcgaacatc gtagacactc aattgaaaaa taatgaggtc attcttagtg gagaaatccc
     1081 tgctcggtgt attcaagaat atcgtagtga tttaactttc tttacaaatg gacgtagtgt
     1141 ttgtttaaca gagttaaaag ggtaccatgt tactaccggt gaacctgttt gccagccccg
     1201 tcgtccaaat agtcggatag ataaagtacg atatatgttc aataaaataa cttagtgtat
     1261 tttatgttgt tatataaata tggtttcttg ttaaaaaaga tgaaatattt tttaataa
//
LOCUS       DAZKNO010000410         1281 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.298, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000410 DAZKNO010000000
VERSION     DAZKNO010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.298"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1255
                     /locus_tag="ACG8MY_002919"
                     /pseudo
     CDS             128..1255
                     /locus_tag="ACG8MY_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
BASE COUNT          426 a          195 c          247 g          413 t
ORIGIN      
        1 caattatcat tttcaattgc attttataaa tcattttgta tgtaatttgg tatgtaagtt
       61 aaaatgaaaa aagtagtcca ttttgaagta cattttgtca ttttgtactc caaaactgaa
      121 ctacttactt gcatatatac gcctttatag tggagtacta catttacgag attcggttag
      181 agtatcagaa aaagaaaaaa taaaagttac agaaatgtat acttcaataa atggtgaatt
      241 atgtaagatt gatagagctt attctggaga aattgttatt ttgcaaaatg agtttttgaa
      301 gttaaatagt gttcttggag atacaaaact attgccacag agaaaaaaga ttgaaaatcc
      361 gcaccctcta ctacaaacaa ctgttgaacc gagtaaacct gaacagagag aaatgttgct
      421 tgatgccctt ttggaaatct cagatagtga tccgcttcta cgatattacg tggattctac
      481 gacacatgaa attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact
      541 gttgcaagaa aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga
      601 gagaccgtta aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg
      661 ggcttccatt ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag
      721 ctcggtttct cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg
      781 ctatggttgc gaacaaggat tatatggttg gaatgtgacg gattgtaaaa tctgttttaa
      841 gtacggttta tactatagcc ctgttagtac tccagcagat tttcggatgc ttactcctat
      901 tgtactggag caagccttta gaaaagctgg aacagaattg ttagagccat atcttagttt
      961 taaagtttat gcaccacagg aatatctttc acgggcatat aacgatgctc ccaaatattg
     1021 tgcaaatatc gtaaatactc aactgaaaaa taatgaggtc attattattg gagaaattcc
     1081 tgctcgatgt attcaagatt atcgcaatga tttaactttt tttacaaatg ggcttagtgt
     1141 ttgtttagca gagctaaaag gatatcaggt taccactggc gaacctgttt gccagacccg
     1201 tcgtctaaat agtcggatag ataaagtaag atatatgttc aataaaataa cttagtgcgt
     1261 tttatgttgt tatataaata t
//
LOCUS       DAZKNO010000411         1107 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.307, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000411 DAZKNO010000000
VERSION     DAZKNO010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.307"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1103
                     /locus_tag="ACG8MY_002920"
                     /note="aph(2'')-Ia"
     CDS             <1..1103
                     /locus_tag="ACG8MY_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFQ0432005.1"
                     /translation="TRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIE
                     DLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSV
                     AYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILG
                     YKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEE
                     YILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLT
                     GIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEY
                     YPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          472 a           83 c          185 g          367 t
ORIGIN      
        1 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
       61 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt
      121 ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt
      181 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
      241 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      301 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      361 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      421 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      481 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      541 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      601 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      661 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      721 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      781 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      841 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      901 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      961 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
     1021 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
     1081 ataaaaggac ttataaagat tgattat
//
LOCUS       DAZKNO010000412          840 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3506
            SAMEA115706764-rid22575813.guided.326, whole genome shotgun
            sequence.
ACCESSION   DAZKNO010000412 DAZKNO010000000
VERSION     DAZKNO010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706764
            Sequence Read Archive: ERR13196498
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287769.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287769.1
            Genome Coverage       :: 167x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 09:05:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,921
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,766
            CDSs (with protein)               :: 2,766
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 53 of 96
            Pseudo Genes (internal stop)      :: 23 of 96
            Pseudo Genes (multiple problems)  :: 19 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706764-rid22575813.guided.326"
                     /isolate="VRE3506"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            227..775
                     /gene="aac(6')"
                     /locus_tag="ACG8MY_002921"
     CDS             227..775
                     /gene="aac(6')"
                     /locus_tag="ACG8MY_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0432006.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          300 a          125 c          179 g          236 t
ORIGIN      
        1 agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga
       61 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
      121 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
      181 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
      241 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
      301 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
      361 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
      421 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
      481 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
      541 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
      601 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
      661 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
      721 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
      781 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
//
